{ "genome": { "genus": "Metamycoplasma", "species": "hominis", "strain": "SP10291", "taxon": "Metamycoplasma hominis SP10291", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 750518, "gc": 0.2693473041286152, "n_ratio": 0.0, "n50": 750518, "n90": 750518, "coding_ratio": 0.7308112530279087 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 472, "stop": 621, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTCAAGCAAAAATGGAAGAATTCAACCAAAAGAAAGCCAAAATTGAATCCAAATATGCTGCTTACAAAGGCGACAGACAAATGATGCAACGTAAGCAAGCTGAAATTAGTGAATTATATAAGAAAGAAAAAATCTCTCCTTGA", "aa": "MFSQAKMEEFNQKKAKIESKYAAYKGDRQMMQRKQAEISELYKKEKISP", "aa_hexdigest": "7588ca6ca96a15cae5b57833b385386d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5875.7765, "isoelectric_point": 9.646807289123537 }, "id": "LMJBGEANGM_43", "locus": "GCA013368015_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1300, "stop": 1434, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATGAAAAGGGAAATTACAAAACCAAAAATTACACTCCATTAACCAAATTCAGTGAAATTCTAATTCCTAATTCTTTATCGTTTGACACTAAGCCAAAAGATAAGGATGGCATTATACCAACAGATTGA", "aa": "MGDEKGNYKTKNYTPLTKFSEILIPNSLSFDTKPKDKDGIIPTD", "aa_hexdigest": "00a76826a52447d9c1fa25fe72451b42", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.553500000002, "isoelectric_point": 6.104928398132324 }, "id": "LMJBGEANGM_44", "locus": "GCA013368015_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1513, "stop": 1854, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTATCGGTTTAGTTGGTTGTGTACAATCATTTACTGTAAAATCAAGTAATAAAGTCGGTTCTGGACAAGAGCTATATTCTAAAAAGAGTGACATTTCACCAAATATTGTCACTTATAGATACGATAGCACTGGTAATTTCTTTACCTTACCAAACAAAATAAATGATAAAAATATTATCGCCAATACATATCTTGCTTTAAAAGAACCTAATGCTATTAATGAGTTAAGGAAGAGAGATCCTAATTATGGCGCATATGGTTCTCGTTCATTTGGATTGCAATTGCAAATGCCAAAAAACGACAAAGCAAAAGTTCTATCTTCTCATGATGAAGATTGA", "aa": "MAIGLVGCVQSFTVKSSNKVGSGQELYSKKSDISPNIVTYRYDSTGNFFTLPNKINDKNIIANTYLALKEPNAINELRKRDPNYGAYGSRSFGLQLQMPKNDKAKVLSSHDED", "aa_hexdigest": "200f4ab9d28dac35760f2bb7d475a211", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12536.983699999986, "isoelectric_point": 9.211774635314942 }, "id": "LMJBGEANGM_45", "locus": "GCA013368015_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1970, "stop": 2299, "strand": "-", "frame": 2, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "RefSeq:WP_020002379.1", "SO:0001217", "UniParc:UPI000368424E", "UniRef:UniRef100_A0A454C8Q8", "UniRef:UniRef50_A0A448ZZD2", "UniRef:UniRef90_D1J7D9" ], "nt": "ATGATAAATAAAAATAACGTTGTTAAAAAAAATTGGGAATTTCAAAAAATTCTTGATTCTCACAATCAAGTGATTTCAAGAAATTTAATAATTTATTTTAACAAATCCGATTTCTTTAAAGTGGGTATTTCAATCCCTAAGCAATTTGCAAATGCTGTTTTGAGAAATCATTACAAAAATCAAATCAGATCAATTCTAAGAAACATTGATTTTAGCACTTTAAATTACGAAACTGTGATTATTGCTCGCAAAGGCTTCTTGAGTCTTGACTGGAATCAAAAAGTTAAAGAAGTAAAAAAACTTTATGAAAGGATCGCTGATGGCAAATAA", "aa": "MINKNNVVKKNWEFQKILDSHNQVISRNLIIYFNKSDFFKVGISIPKQFANAVLRNHYKNQIRSILRNIDFSTLNYETVIIARKGFLSLDWNQKVKEVKKLYERIADGK", "aa_hexdigest": "5aaf002c4988c04a976148e8c285e553", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000368424E", "ncbi_nrp_id": "WP_020002379.1", "uniref100_id": "UniRef100_A0A454C8Q8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000368424E", "RefSeq:WP_020002379.1", "UniRef:UniRef100_A0A454C8Q8" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C8Q8", "uniref90_id": "UniRef90_D1J7D9" }, "psc": { "uniref90_id": "UniRef90_D1J7D9", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A448ZZD2", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZD2" ], "product": "Ribonuclease P protein component" }, "genes": [ "rnpA" ], "id": "LMJBGEANGM_46", "locus": "GCA013368015_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2327, "stop": 2473, "strand": "-", "frame": 2, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_012855284.1", "SO:0001217", "UniParc:UPI00006920BF", "UniRef:UniRef100_D1J7E0", "UniRef:UniRef50_P05647", "UniRef:UniRef90_B3PNJ7" ], "nt": "ATGAAAAGAACATACCAACCAAAAAAACGCAAACACATTAAAGTTCATGGTTTTATGGCTAGAATGCAAACTAAAAATGGTAGAAAAGTTTTAGCAGCTAGACGTGCAAAAGGTAGATATACATTAACTGTATCAGACAATAAATAA", "aa": "MKRTYQPKKRKHIKVHGFMARMQTKNGRKVLAARRAKGRYTLTVSDNK", "aa_hexdigest": "c12d043f3061d758a33db3c492b0b7ea", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00006920BF", "ncbi_nrp_id": "WP_012855284.1", "uniref100_id": "UniRef100_D1J7E0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00006920BF", "RefSeq:WP_012855284.1", "UniRef:UniRef100_D1J7E0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E0", "uniref90_id": "UniRef90_B3PNJ7" }, "psc": { "uniref90_id": "UniRef90_B3PNJ7", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "LMJBGEANGM_47", "locus": "GCA013368015_00005" }, { "type": "oriC", "sequence": "contig_1", "start": 2474, "stop": 2804, "strand": "?", "product": "origin of replication", "nt": "AACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGTATATAAAATACCTAAGAAAAAAAATTTAATATTTTTTATGTTTTTAATGTAAATTAAAAGAAACACAAAATTCAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAACCTCTTATTTTAAAAACAAAAGCCCTAAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGTTTATTAATTTATTAATTTTGAATTTTAAAATATAATAGAGGT", "id": "LMJBGEANGM_1293" }, { "type": "cds", "sequence": "contig_1", "start": 2805, "stop": 3734, "strand": "+", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A448ZWT6", "UniRef:UniRef90_D1J7E1" ], "nt": "ATGAAAACTACAAATCCCTCTGAATTAGATTTAAGAACACATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCTTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGACGTTTTAACATATATTAAGAATAGTCATAACGAAAGTATTAAAAGAGCAATTAAAAACGTTTATGGTGAAGAATATAATTTGGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTGCAAGATAGCAATAATGTTGAATCTGCAACTAATTCATTGACTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAACATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTTATTCATGCTTCAAGTGGGCTTGGTAAAACACATCTATTACATGCTATCGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTAATGTCTTATGATTGCCTAATGTTTGATGATGTTCAACAATATGGTAATCGTGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAGCCGGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATATCTTGAAATTTAAGTTGCAAGAAAACGGTATAAATCCGGAACTTTGA", "aa": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESATNSLTNNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINILKFKLQENGINPEL", "aa_hexdigest": "63a2a287cb920233edfea50c1364b362", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "3-prime", "pseudogene": { "start": 2805, "stop": 4037, "observations": { "mutation": [ 3732 ], "stop": [ 3732 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E1", "query_cov": 1.0, "subject_cov": 0.6673866090712743, "identity": 0.997, "score": 602.0, "evalue": 4.08e-215, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESATNSLANNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINILKFKLQENGINPELWEEESLKFIARNFSSSIRNIEGAINRIKLFQDGDDGFFTYDINTIKSIFNSMTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLNWIEKNANSNPTLKLALQKIESNLKKIS" }, "paralog": false, "identity": 0.9951219512195122, "score": 803.0, "evalue": 1.09e-296, "description": "Internal stop codon at 3732. Nonsense mutation around 3732.", "psc": { "uniref90_id": "UniRef90_D1J7E1", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A448ZWT6", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E1", "UniRef:UniRef50_A0A448ZWT6", "COG:COG0593", "COG:L", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWT6" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "LMJBGEANGM_48", "locus": "GCA013368015_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3885, "stop": 4115, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGACTCAAGTTGCTGAGAATATTACCCCCGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGATGTGGTTAATGCCCGTAGAATAGCAACATATTTAGTGAATAAAAATTTTAATTATACATTGCAAGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCGCTTAATTGA", "aa": "MTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLN", "aa_hexdigest": "28ae5fe87d266549f489ee290f7117cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 66, "aa_cov": 0.868421052631579, "hmm_cov": 1.0, "evalue": 2.5559678325857753e-21, "score": 71.04753875732422, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8637.7939, "isoelectric_point": 9.753437995910645 }, "id": "LMJBGEANGM_49", "locus": "GCA013368015_00007" }, { "type": "cds", "sequence": "contig_1", "start": 4212, "stop": 4610, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00712.24" ], "nt": "ATGAAATTAAAAATTGATAAATTGTTATTGGATAATGCAATTGAAAGAGTATCAAAATCCGTGGATGCTAATCCATTTTTACCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAGCATGAAATAGAAACATCGATTGATGCCCAAATTGAATTGCCTGGAATTGTATTAGTTGAACTTAGCTTATTTAAAAATATTATTAAAAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATAAGTACAAACAACGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATTGATTTTGCATGTTATGGGGAACAAGTAAAAATTAACTGA", "aa": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSIDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKIN", "aa_hexdigest": "9374d5d019b57ee755228d26d12ce06b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00712.24", "name": "DNA_pol3_beta", "length": 122, "aa_cov": 0.9090909090909091, "hmm_cov": 0.9754098360655737, "evalue": 1.6165211542919434e-18, "score": 62.551979064941406, "start": 1, "stop": 120 } ], "seq_stats": { "molecular_weight": 14786.951699999983, "isoelectric_point": 4.6782129287719725 }, "id": "LMJBGEANGM_50", "locus": "GCA013368015_00008" }, { "type": "cds", "sequence": "contig_1", "start": 4677, "stop": 5309, "strand": "+", "frame": 3, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0", "UniRef:UniRef90_D1J7E2" ], "nt": "TTGTGCTGTGTCAATATATCGGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCCCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAACTTTGATTACAATGGCGACGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAGTCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACTGAACTTAAAATAACCAAAAAAGAACTAAATAACTTATTGAATAAGGCATCAGTTATTATTAATGAAAACTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAACCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGCGAAGAATTGAAACTCGCATTAAATAGTAGATTCTTAAAGGATGCTATTTCTGTATATGAAGATAATTTGAATATTTTATTTACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAA", "aa": "MCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLTISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF", "aa_hexdigest": "5fd6dec55127547b14c9da8b30a299ed", "start_type": "TTG", "rbs_motif": null, "genes": [ "dnaN" ], "truncated": "5-prime", "pseudogene": { "start": 4377, "stop": 5309, "observations": { "mutation": [ 4608 ], "stop": [ 4608 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E2", "query_cov": 1.0, "subject_cov": 0.5753424657534246, "identity": 0.995, "score": 399.0, "evalue": 5.1e-138, "gene_start": 1, "gene_end": 210, "reference_start": 156, "reference_end": 365, "reference_sequence": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSIDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKINWNDFKSLVRDVIFAASTNEVSLILCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLTISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF" }, "paralog": false, "identity": 0.9967741935483871, "score": 593.0, "evalue": 7.94e-217, "description": "Internal stop codon at 4608. Nonsense mutation around 4608.", "psc": { "uniref90_id": "UniRef90_D1J7E2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A2Z4LLI0", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E2", "EC:2.7.7.7", "UniRef:UniRef50_A0A2Z4LLI0", "COG:COG0592", "COG:L", "KEGG:K02338", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0" ], "product": "DNA polymerase III subunit beta" } }, "id": "LMJBGEANGM_51", "locus": "GCA013368015_00009" }, { "type": "cds", "sequence": "contig_1", "start": 5309, "stop": 5482, "strand": "+", "frame": 2, "gene": "ybcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "db_xrefs": [ "COG:COG2501", "COG:J", "SO:0001217", "UniRef:UniRef50_N9UZE0", "UniRef:UniRef90_D1J7E3" ], "nt": "ATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGTCCAACGGGCGGAATGGCAAAATATTTTGTTCAGGTTCATAGAATATTAATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGA", "aa": "MEIEITGEFIKLSQFLKKIDVCPTGGMAKYFVQVHRILINGNLANGRNAKIYPGDTV", "aa_hexdigest": "c366bbf7c6e153c1c5ccd4e3f3610b33", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7E3", "query_cov": 1.0, "subject_cov": 0.8142857142857143, "identity": 1.0, "score": 119.0, "evalue": 4.16e-34, "valid": true, "gene": "YbcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "uniref50_id": "UniRef50_N9UZE0", "cog_id": "COG2501", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_N9UZE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZE0" ], "product": "RNA-binding S4 domain-containing protein" }, "genes": [ "ybcJ" ], "id": "LMJBGEANGM_52", "locus": "GCA013368015_00010" }, { "type": "cds", "sequence": "contig_1", "start": 5590, "stop": 5787, "strand": "+", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855288.1", "SO:0001217", "UniParc:UPI0001BC1FC5", "UniRef:UniRef100_D1J7E4", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_B3PLS0" ], "nt": "ATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAG", "aa": "MPGRDQLTGQKALSGNKRSHALNTAKRTFDLNLQKVTVLTETGSKKTVRVTAKNARTLKKYGLVA", "aa_hexdigest": "be79e6e221684802b48e19dfde4c6d71", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC1FC5", "ncbi_nrp_id": "WP_012855288.1", "uniref100_id": "UniRef100_D1J7E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FC5", "RefSeq:WP_012855288.1", "UniRef:UniRef100_D1J7E4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E4", "uniref90_id": "UniRef90_B3PLS0" }, "psc": { "uniref90_id": "UniRef90_B3PLS0", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "LMJBGEANGM_53", "locus": "GCA013368015_00011" }, { "type": "cds", "sequence": "contig_1", "start": 5858, "stop": 6025, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAATACAAGCGTAAAACTTTATTAAATAATTTTCCAAGCAATTTAAAACAAAAAATTTCTGATTTTTTAAAAAGCAATGAACTAGATTTAAATATAAGAGCTGAAAAAATTACAAAAGAAATTTCTATTAATTTATTCAAATTTATAAACAATAAAAATTAA", "aa": "MFQYKRKTLLNNFPSNLKQKISDFLKSNELDLNIRAEKITKEISINLFKFINNKN", "aa_hexdigest": "9b39046340e955f50bf66d3288005cc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6604.633099999999, "isoelectric_point": 9.996741676330569 }, "id": "LMJBGEANGM_54", "locus": "GCA013368015_00012" }, { "type": "cds", "sequence": "contig_1", "start": 6062, "stop": 6631, "strand": "-", "frame": 2, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0006364", "SO:0001217", "UniRef:UniRef50_D1J7E5", "UniRef:UniRef90_D1J7E5" ], "nt": "ATGATTAAAGCATTAAAATCATTGGGCCAAAATTTCTTAAGCAATAAAAACATTCAAAAAGAGATTGTAAAAGTTGCGAACGTTGCAGGCAAAAATATTATTGAAATTGGCCCAGGAATGGGCGCAATAACTGATCAAATGGCAAATGTTGTAAATAGACTTGTATGTATTGAATTTGATAAAAGATTATACGAATTTTTATTACAAAAAAATTATTCAAGCAATGTTGAAATATTGAACCAAGATTTCTTACAAACAGATTTATTAAAATATACTGATTTTGAAGTAATTGGAAACATTCCTTATAATATAACAAGCGATATAATTTTTAAATTATTAGATAACGCTAAAAACATTAATAAAATAACATTAATGGTTCAAAAAGAAGTAGCGGATAGAATATGCTGTACAGCCGGTAATTCTCAATATAGTAAATTAGCAGCATCAATACAACTTCTCTATGAACCTAAATATTTATTCACTGTTAAGGCAAAAGAATTTAATCCCGCTCCTAAAGTTGATTCGGCAGTTATTCAACTAAATCTAATAAAAAATAATGATTTTATATGA", "aa": "MIKALKSLGQNFLSNKNIQKEIVKVANVAGKNIIEIGPGMGAITDQMANVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAKNINKITLMVQKEVADRICCTAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLNLIKNNDFI", "aa_hexdigest": "a0baab756ee7cba7cc54b2d14a63d0bf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rsmA" ], "truncated": "5-prime", "pseudogene": { "start": 5858, "stop": 6655, "observations": { "mutation": [ 6064 ], "stop": [ 6064 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E5", "query_cov": 1.0, "subject_cov": 0.7132075471698113, "identity": 0.9740000000000001, "score": 360.0, "evalue": 2.27e-124, "gene_start": 1, "gene_end": 189, "reference_start": 9, "reference_end": 197, "reference_sequence": "MLERFLKSMIKALKSLGQNFLSNKNIQKEIVKAANVAGKNIIEIGPGMGAITDQMADVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAENINKITLMVQKEVADRICCGAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLILIKNNDFIWNNQVEILKFIKQMFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN" }, "paralog": false, "identity": 0.9735849056603774, "score": 500.0, "evalue": 3.05e-182, "description": "Internal stop codon at 6064. Nonsense mutation around 6064.", "psc": { "uniref90_id": "UniRef90_D1J7E5", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_D1J7E5", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E5", "EC:2.1.1.182", "UniRef:UniRef50_D1J7E5", "COG:COG0030", "COG:J", "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7E5" ], "product": "Ribosomal RNA small subunit methyltransferase A" } }, "id": "LMJBGEANGM_55", "locus": "GCA013368015_00013" }, { "type": "cds", "sequence": "contig_1", "start": 6624, "stop": 7391, "strand": "-", "frame": 1, "gene": "tatD", "product": "YchF/TatD family DNA exonuclease", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.21.-", "KEGG:K03424", "RefSeq:WP_175936613.1", "SO:0001217", "UniParc:UPI00158C8F42", "UniRef:UniRef100_UPI00158C8F42", "UniRef:UniRef50_N9U095", "UniRef:UniRef90_A0A6A8Q279" ], "nt": "ATGAAATTTATCGATTTACACACCCACCCATCAACCGAATACTATTCTGATCCTCTTGAAATAGTTAATCATTGGCATAACCTAAATATGGAAAAGCTTTTTGTATGTGGAACAAACGAAGATGATTCGATACAAGCAATAGAACTTGCTAAAAAAGTTGATTTTGTTTATCCTGTTATTGGAATACATCCAACATTATCAAATGGTTTGCAAGATAGAGAAATTTTAAACAAGATATATGAACCAAATGTTGTTGCAATTGGAGAAGTGGGCCTTGATTATCATTATGATGATTCGCCTAGCAAAGAAATTCAAAAACAAGGATTTATTGCCCAAATAGATTTTGCAAAAGAACATAATTTACCAGTAATTATGCACATTAGAGATGCTTTAGATGATTCTTTAGATATAATAACCTTGCCTCAATATAAAGACATTAATTTTATATTTCACTCATTTAGCGGGGATCTTAGTTTTTTAAATAAATGTTTGAAATATTCAAATATCTATTATTCAATAAGTGGGGTTGTTACTTTTAAAAATGCTAAGCCTTTACAAGAGGCAGTAAAAAATATTCCTTTAGATAGAATATTTTGTGAAACTGATACACCTTATTTAGCCCCAACTCCAATGAGAGGAAAACCCAACATTAGCCCTTTTGTTCAATACACATATGAATACATTGCTAATTTAAAACAAATTGATATTGATGTTTTAGTTGAACAAATTAAAAAGAATGTTAGAAAGGTTTTTAAAATCTATGATTAA", "aa": "MKFIDLHTHPSTEYYSDPLEIVNHWHNLNMEKLFVCGTNEDDSIQAIELAKKVDFVYPVIGIHPTLSNGLQDREILNKIYEPNVVAIGEVGLDYHYDDSPSKEIQKQGFIAQIDFAKEHNLPVIMHIRDALDDSLDIITLPQYKDINFIFHSFSGDLSFLNKCLKYSNIYYSISGVVTFKNAKPLQEAVKNIPLDRIFCETDTPYLAPTPMRGKPNISPFVQYTYEYIANLKQIDIDVLVEQIKKNVRKVFKIYD", "aa_hexdigest": "6fd271749c971da68efb1278071a7e85", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158C8F42", "ncbi_nrp_id": "WP_175936613.1", "uniref100_id": "UniRef100_UPI00158C8F42", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C8F42", "RefSeq:WP_175936613.1", "UniRef:UniRef100_UPI00158C8F42" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C8F42", "uniref90_id": "UniRef90_A0A6A8Q279" }, "psc": { "uniref90_id": "UniRef90_A0A6A8Q279", "gene": "TatD", "product": "YchF/TatD family DNA exonuclease", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_N9U095", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424" }, "pscc": { "uniref50_id": "UniRef50_N9U095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U095" ], "product": "TatD DNase family protein" }, "genes": [ "tatD" ], "id": "LMJBGEANGM_56", "locus": "GCA013368015_00014" }, { "type": "cds", "sequence": "contig_1", "start": 7391, "stop": 7564, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTTGTTCATTGGCACATACATCATTTATTTTTGGCAAACCATTTTATTCATTCAAGGTAATTTCAGATTGTTTGGAATCTAATAATAACCTAGAATATAGGCAATCAAAAGCAATTTGGGAATTAAAAATAATTGATATTTTTAAAGATATATTAGGAGAACTATAA", "aa": "MECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDILGEL", "aa_hexdigest": "aef54c524208904dd78ed868a5e09c2e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6649.642099999997, "isoelectric_point": 5.122978019714354 }, "id": "LMJBGEANGM_57", "locus": "GCA013368015_00015" }, { "type": "cds", "sequence": "contig_1", "start": 7589, "stop": 7924, "strand": "-", "frame": 2, "gene": "mtnN", "product": "Nucleoside phosphorylase domain-containing protein", "db_xrefs": [ "COG:COG0775", "COG:FH", "SO:0001217", "UniRef:UniRef50_A0A454C9F7", "UniRef:UniRef90_A0A454C9F7" ], "nt": "TTGTTAGCGCACTGTGGAGTTGGAAAAGCTAATTCTGCTTTAATGCTAAGTTATTTATTGACTAAGTTCAATATATTAAAAGTAATTAACATTGGACCTGCTATATCAATTTTCAATGGTAGCGTTGGAGATATTTATTGCATACAAAATTGCAAATATTATGACGTTAATTTAACCGCCTTACCTAATTATAAAATTGGGCAATTGCCTAGTTTGCAAGATGTATTTCATTGCAATCAAGATCTAAAAATTTGCAGCAATTTTAAAAAAGCCAATATTATAACCGGCGACAGTTTTATTAATAAAAGCATAATAAATTCAGATATATTAGATTGA", "aa": "MLAHCGVGKANSALMLSYLLTKFNILKVINIGPAISIFNGSVGDIYCIQNCKYYDVNLTALPNYKIGQLPSLQDVFHCNQDLKICSNFKKANIITGDSFINKSIINSDILD", "aa_hexdigest": "815430c58337e2dc6543c6bde4c5f014", "start_type": "TTG", "rbs_motif": null, "genes": [ "mtnN" ], "truncated": "both", "pseudogene": { "start": 7391, "stop": 8077, "observations": { "mutation": [ 7591, 7942 ], "stop": [ 7591, 7942 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F7", "query_cov": 1.0, "subject_cov": 0.4868421052631579, "identity": 0.982, "score": 222.0, "evalue": 8.21e-72, "gene_start": 1, "gene_end": 111, "reference_start": 52, "reference_end": 162, "reference_sequence": "MEKKFYLFIFAEPLETEQLRIKYSKHLKQIKIDFENNFQNLYELNWNNKQILLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGSVGDIYCIQNCKYYDVNLTALPNYKIGQLPSLQDVFHCNQDLKICSNFKKANIITGDSFINKSIINSDILDWNTNNLLGDMECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDILGEL" }, "paralog": false, "identity": 0.9736842105263158, "score": 452.0, "evalue": 4.22e-165, "description": "Internal stop codon at 7591, 7942. Nonsense mutation around 7591, 7942.", "psc": { "uniref90_id": "UniRef90_A0A454C9F7", "gene": "MtnN", "product": "Nucleoside phosphorylase domain-containing protein", "uniref50_id": "UniRef50_A0A454C9F7", "cog_id": "COG0775", "cog_category": "FH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F7", "UniRef:UniRef50_A0A454C9F7", "COG:COG0775", "COG:FH" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F7" ], "product": "Nucleoside phosphorylase domain-containing protein" } }, "id": "LMJBGEANGM_58", "locus": "GCA013368015_00016" }, { "type": "cds", "sequence": "contig_1", "start": 7940, "stop": 8077, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGAAATTTTATTTATTTATTTTTGCCGAACCACTAGAAACTGAACAATTAAGGCTAAAATATAGCAAGCATTTAGAACAAATAAAAATTGATTTTGAAAACGATTTTCAGAATCTTTATGAATTAAATTGA", "aa": "MEKKFYLFIFAEPLETEQLRLKYSKHLEQIKIDFENDFQNLYELN", "aa_hexdigest": "1de102577b29c30ef98625e71a6b783d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5657.4048999999995, "isoelectric_point": 4.910002326965332 }, "id": "LMJBGEANGM_59", "locus": "GCA013368015_00017" }, { "type": "cds", "sequence": "contig_1", "start": 8389, "stop": 8514, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAGGCAAATATAAGGTTTTAAACGACATTCTATTGCAAATTGATAATGATAAAACAGCTCAAATAGATCATATTGTTGTTTCAACTTACGGTATTTTTGTAATTGAAACAAAGAATTGA", "aa": "MNEGKYKVLNDILLQIDNDKTAQIDHIVVSTYGIFVIETKN", "aa_hexdigest": "fc16c759b92a451d72e0b936a87dbd2a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4694.318299999999, "isoelectric_point": 4.889369773864746 }, "id": "LMJBGEANGM_60", "locus": "GCA013368015_00018" }, { "type": "tRNA", "sequence": "contig_1", "start": 9162, "stop": 9248, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 74.5, "nt": "GCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 9195, 9197 ], "id": "LMJBGEANGM_1", "locus": "GCA013368015_00019" }, { "type": "tRNA", "sequence": "contig_1", "start": 9254, "stop": 9329, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 80.1, "nt": "GCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 9286, 9288 ], "id": "LMJBGEANGM_2", "locus": "GCA013368015_00020" }, { "type": "cds", "sequence": "contig_1", "start": 9567, "stop": 9752, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17676.7" ], "nt": "TTGAAACCTAATGAACTATGTGATAAGGTTTTATTTCTTGAAACAAGTGAAGAAAAGCCAAACCCGCAATTACTTAAAAAGATGTTGCTAAAATTCAAAGAAGTTGGTTTATTTAAAAATATTCAAGGAATATTAGTCGGATGCCCTCAAAATCGCGTTTATTATAATGAATATAAAGAAGTTTGA", "aa": "MKPNELCDKVLFLETSEEKPNPQLLKKMLLKFKEVGLFKNIQGILVGCPQNRVYYNEYKEV", "aa_hexdigest": "25b6ae2d5c9d0c717ddabe1a5727625c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17676.7", "name": "Peptidase_S66C", "length": 50, "aa_cov": 0.7704918032786885, "hmm_cov": 0.96, "evalue": 4.977775746365193e-10, "score": 35.6115837097168, "start": 4, "stop": 50 } ], "seq_stats": { "molecular_weight": 7189.4410000000025, "isoelectric_point": 8.597842216491703 }, "id": "LMJBGEANGM_61", "locus": "GCA013368015_00021" }, { "type": "cds", "sequence": "contig_1", "start": 10005, "stop": 10493, "strand": "-", "frame": 1, "gene": "mccF", "product": "Microcin C7 resistance MccF related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "nt": "ATGATCAAACCAATAAAATTAAGCAAGGAAAATGGCATTGCAATAGTAAGTTTATCTGCAGGAACTATTGGAGAAGAATTTGCTAGTCACCAATTAGATTTAGCAATAAAAAGACTAAATTTAGATTTTCAACTTAACGTTATGATTGCTAAAAACGCACTTAAGGGAATAAAAGTTTTAAAAGAAAATCCTATGTTAAAAGTTGAGGATTTAAATGAAGTTTTTAGCAATGTCAATGTAAAAGCAATAATTTGTGCAATTGGTGGATTTGACACATACAAAACTATTCCACTTTTATTAGAAGACGAAAACTTTAAAAATTTAGTCTTAAATAATCCTAAAATTTTTATTGGATATTCTGATTCAACCGTTAACCATTTAATGTTTTATAGATTAGGACTAAAAACATTTTATGGGCACACCATTTTATGTGATATTGCTGAACTTGACAAAGAAATGTTGCCTTATACTAAAAACTCATTTAAATGA", "aa": "MIKPIKLSKENGIAIVSLSAGTIGEEFASHQLDLAIKRLNLDFQLNVMIAKNALKGIKVLKENPMLKVEDLNEVFSNVNVKAIICAIGGFDTYKTIPLLLEDENFKNLVLNNPKIFIGYSDSTVNHLMFYRLGLKTFYGHTILCDIAELDKEMLPYTKNSFK", "aa_hexdigest": "6886e98c82bd79d2a64bcbd8a68d7fca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00308136E8", "query_cov": 0.9938271604938271, "subject_cov": 1.00625, "identity": 0.5529999999999999, "score": 184.0, "evalue": 3.97e-57, "valid": false, "product": "LD-carboxypeptidase", "uniref50_id": "UniRef50_X0PHG8" }, "pscc": { "uniref50_id": "UniRef50_X0PHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "product": "Microcin C7 resistance MccF related protein" }, "genes": [ "mccF" ], "id": "LMJBGEANGM_62", "locus": "GCA013368015_00022" }, { "type": "cds", "sequence": "contig_1", "start": 10584, "stop": 11234, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGAAACTAATGAAATATTTATTGACCCTGATCAAATAGAAAAAAATAATGTTAAACTAAACAAAACAGAAAATTATGCATATAAAGATTTAAACGATAGACTTCATCATTTTGATTCAAATCCCGAAACATTATTCATTAACGAAGCAAAGAAAATAATTAGTGAATATAAAAATGAAATAAACGTCAAAGTTTATACAAAAAACCCTGTTTTTCATGGTTTAAAATTAGAATATTTTGATGAAGAAAGAGAAATTAAGTCTTCATATCCTGATTTTGTTTTTAAAATAAATAATGGTGAAGTAACAAATTATTTATATATTGAAGTTAAAAGCGCAAAACACGATTATAATCAACAAAAAACTAAAAACATAGTTACTAGCTATAAAAAATATTTAGACAATAAAAAACCTGATAATGCTTTATTTGTTGATGACGTAGAAAAAAATAACAATTACACGTTTTTAATTTGCTATGTTGATGAAGAAAACCAAGACTTTTATTTTTTAGGGGCAAGTGATAATGCAGAACTTGATAAAGAGGTCAATATATCTATTCCTGATGGCATAAAAGAATTAATTATCAATAAAAATAAATTTATTTCAGATATTGAATCATTATTCAAATATTTTCAAAACAATAAATAA", "aa": "MPETNEIFIDPDQIEKNNVKLNKTENYAYKDLNDRLHHFDSNPETLFINEAKKIISEYKNEINVKVYTKNPVFHGLKLEYFDEEREIKSSYPDFVFKINNGEVTNYLYIEVKSAKHDYNQQKTKNIVTSYKKYLDNKKPDNALFVDDVEKNNNYTFLICYVDEENQDFYFLGASDNAELDKEVNISIPDGIKELIINKNKFISDIESLFKYFQNNK", "aa_hexdigest": "4ed42adfcd949a63e3cfe3d24bc3c133", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25638.308100000027, "isoelectric_point": 5.006060218811036 }, "id": "LMJBGEANGM_63", "locus": "GCA013368015_00023" }, { "type": "cds", "sequence": "contig_1", "start": 11493, "stop": 11894, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTGCAACTTAGAAAAGTGTCATCAAGTAGTTTGTCTATTCAAACAATAGGAAGAATTAAAAGATTCCCAAATCCAAGTTATAACAAAGATCAAATTTCTAGAGAAGGCAATAGATACTATATTTATTCAAATGCTCAAACCAAAGAAAATATAAGAAGAACATTGATTTTAAAACGAGAATTTGAAAATGAAAAATTTGTTTATGGCCAAATAAATAAAGTAAAAATCAAACAAGCAATAATAGGAGCAGATTTTATTAGAGAAGCTAAAAATTCTATTGTTAAAGAAAAGATTGATGAATATTTTAAAAAATACCATAAAGAATATATTAAAAACAACAAGAAATTAATAGGAGAACAAGGAAACATTGAAGATAAAATTAGAGTTTATTCATGA", "aa": "MLVQLRKVSSSSLSIQTIGRIKRFPNPSYNKDQISREGNRYYIYSNAQTKENIRRTLILKREFENEKFVYGQINKVKIKQAIIGADFIREAKNSIVKEKIDEYFKKYHKEYIKNNKKLIGEQGNIEDKIRVYS", "aa_hexdigest": "beb3cf193d9d6585f06e5659a021583d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15826.048599999987, "isoelectric_point": 9.932660102844238 }, "id": "LMJBGEANGM_64", "locus": "GCA013368015_00024" }, { "type": "cds", "sequence": "contig_1", "start": 11913, "stop": 13037, "strand": "-", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "nt": "ATGAATCTAACTAGTGTACAAGAAAAAGCAGTTAAACAATTGCTTGAAGAATATAAAATCGCAATTCAATCTAGCGAACCTAAAATAGTTGATTTTCAAGCTCCAACTGGTAGCGGTAAAACCTTTATGTTGGCCAACACTATAAATGAAATAATAAAAGAAAATAATGCCAATGGTGAACCAAATAAATTAGTCTTTGTTATTGCTACACTTTCATCGGCAGATTTACCTTATCAAATGGAATCAAACCTAAATGAATATAAAAGATATATTAATGGTTTGTATGAAGTAGAAAGAAAGGAATCTCCTTCCTTGAATTCTCAAAAAAATAAAGATTCAACATATAATTTAATTCCTCAAAAAAATAAAGTAATAATCTTCGGTGTTTCATCGTTTGGTAAAAATAGAATATTTACCGAACAAGGTTTGCTTGAAAGCTTTCTTGATCAAATTAAATATCAAAACTATACATTAGTTTATATTAGAGATGAAGCGCATCATGGTGGAAATATAAAAGGTAGAAGTAATTTTAAATTTGAAGATATTGATACAATCCATGATGAATCTAAATTAAAAAAAGAAGAAGTTAGATTTGAATATAAAATTCAAAAAGCAGCCAATTTAATAATAAAAATGACAGCAACTCCTAAAGGAACACATAAACTTGTCTATATTGATGAAAAGGATTTAGCCGAAGATGATATTAAATTGTTAAAAGATGAATTAATTTATAACAAGGGTATAGCTGACTCAAGTCAAGATTTAATTGATGATAATGAATTACTAAATAAAGCATGTCAAACATTTAAAGAAGTTCAAATCCAATATGCAAACAATAATAGTGATTTAAAAAATATAAGACCAGCAATGCTTATTCAAGTCGAAAATGAACCAAGTGAAAAATCAACAGCCAAAGAAAAAGATGATTTTAAAGAAAGCATTGAGAACATAATTAAAAAATTAAATGAATGTAATTTGAGTTATGTTAAATACTTTTCAAATGATAAAGTGGAATCTTCTTCTTTGATAAAGGAAGAAAATCATAAAATAAACTTAAAAGAAATTTCAAAAAATGACTCTAATGTTGATGCAATAATTTTTAAAATTGGTCCTTCAACTGGTTGA", "aa": "MNLTSVQEKAVKQLLEEYKIAIQSSEPKIVDFQAPTGSGKTFMLANTINEIIKENNANGEPNKLVFVIATLSSADLPYQMESNLNEYKRYINGLYEVERKESPSLNSQKNKDSTYNLIPQKNKVIIFGVSSFGKNRIFTEQGLLESFLDQIKYQNYTLVYIRDEAHHGGNIKGRSNFKFEDIDTIHDESKLKKEEVRFEYKIQKAANLIIKMTATPKGTHKLVYIDEKDLAEDDIKLLKDELIYNKGIADSSQDLIDDNELLNKACQTFKEVQIQYANNNSDLKNIRPAMLIQVENEPSEKSTAKEKDDFKESIENIIKKLNECNLSYVKYFSNDKVESSSLIKEENHKINLKEISKNDSNVDAIIFKIGPSTG", "aa_hexdigest": "bed27942e44ef3477c85c08b5c884b0b", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0001981E5C", "query_cov": 0.8903743315508021, "subject_cov": 1.021472392638037, "identity": 0.598, "score": 364.0, "evalue": 4.8e-122, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI001E2BAF28" }, "pscc": { "uniref50_id": "UniRef50_UPI001E2BAF28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "LMJBGEANGM_65", "locus": "GCA013368015_00025" }, { "type": "cds", "sequence": "contig_1", "start": 13024, "stop": 13182, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCATTCCTTATATTTTTTACACGGCCAAAGATTTTAAAAACATGTTGAATGATTTTAACTTATCGAATGTAAATAAAATTAACGAACAAGAAATATTACTAAATTTAACCGCTTTAAAACCAATTGAAGGAAAAGATAGCAATGAATCTAACTAG", "aa": "MFIPYIFYTAKDFKNMLNDFNLSNVNKINEQEILLNLTALKPIEGKDSNESN", "aa_hexdigest": "207dd330ef3984d3a4d63f54cadf7b79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6053.822999999999, "isoelectric_point": 4.715101432800293 }, "id": "LMJBGEANGM_66", "locus": "GCA013368015_00026" }, { "type": "cds", "sequence": "contig_1", "start": 13954, "stop": 14121, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCTTGCGCTTATAAATTTATTAATTCTTTATTAAAAATATTAGGTCAAGAAAAATACGAAACTTTGTTTAAAGTTGAGCATAAATTTGACAAAGTTTCACAAAAATTTATAACAGAAAGAACATTATTGATAAGACTTAATAATAATTATGCATTTTAG", "aa": "MKKSCAYKFINSLLKILGQEKYETLFKVEHKFDKVSQKFITERTLLIRLNNNYAF", "aa_hexdigest": "f6c7cb13511b5f83f46efb6f3c18e6d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6631.762699999999, "isoelectric_point": 9.723266792297363 }, "id": "LMJBGEANGM_67", "locus": "GCA013368015_00027" }, { "type": "cds", "sequence": "contig_1", "start": 15297, "stop": 15452, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6", "UniRef:UniRef90_UPI0034A3CAD8" ], "nt": "ATGCAAAACGATTTTTATAATATTGAATTTGTGGGCATTGACGCCTATGGCAGAGCCTTCATCATGAGCAGAGAAGATGCCGAAAGATTTTTTCAAAATCAAGAAATAGAGGAAAACAAATCCTCTAAATCCCAATCAAAATCAAAAACTTTATAA", "aa": "MQNDFYNIEFVGIDAYGRAFIMSREDAERFFQNQEIEENKSSKSQSKSKTL", "aa_hexdigest": "837ca6fd14dec4117e6d022b46bd8a44", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 15126, "stop": 15452, "observations": { "mutation": [ 15171, 15216, 15267 ], "stop": [ 15171, 15216, 15267 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A3CAD8", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 0.98, "score": 95.9, "evalue": 1.33e-24, "gene_start": 1, "gene_end": 51, "reference_start": 58, "reference_end": 108, "reference_sequence": "MAIMTLSEIKKDLDYWKEHEPQDVYTLNDAWELADQELMKYGMEADLWAFDINNIYEMQNDFYNIEFVGIDAYGRAFIMSREDAERFFQNQEIEENKSSESQSKSKTL" }, "paralog": false, "identity": 0.9629629629629629, "score": 204.0, "evalue": 4.67e-70, "description": "Internal stop codon at 15171, 15216, 15267. Nonsense mutation around 15171, 15216, 15267.", "psc": { "uniref90_id": "UniRef90_UPI0034A3CAD8", "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A3CAD8", "UniRef:UniRef50_A0A6A8PZW6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6028.565399999999, "isoelectric_point": 4.893632698059082 }, "id": "LMJBGEANGM_68", "locus": "GCA013368015_00028" }, { "type": "cds", "sequence": "contig_1", "start": 15530, "stop": 15754, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A281B", "UniRef:UniRef90_UPI00193A281B" ], "nt": "GTGTTAGATTTTGATTCTGCATACGAATGGGCAAATCAAGAACTTAAAGAAAAAGGAATGGAGGCGGAGGGGTGGGCATTTGATATTGAAAAAATTTCAAATATAGAAACCTTTAATGTTGAATTTATTGCATTTAATGCTTATAATAGAGCACTTATAATTTCCAGAGAAGATGCACAAAAATATACAGATAACTCTGAAAGAGATAAAGATTATAATGAATAA", "aa": "MLDFDSAYEWANQELKEKGMEAEGWAFDIEKISNIETFNVEFIAFNAYNRALIISREDAQKYTDNSERDKDYNE", "aa_hexdigest": "567c60002717a23bbf030971a90d7c49", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 15461, "stop": 15754, "observations": { "mutation": [ 15506 ], "stop": [ 15506 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A281B", "query_cov": 1.0, "subject_cov": 0.7628865979381443, "identity": 0.973, "score": 144.0, "evalue": 1.59e-43, "gene_start": 1, "gene_end": 74, "reference_start": 24, "reference_end": 97, "reference_sequence": "MAIMTLSEIKRDLDYWKTHSPECVLDFDSAYEWANQELKEKGMEAEGWAFDIEKISNIETFNVEFIAFNAYNRALIISREDAQKYIDNSERDKDYNE" }, "paralog": false, "identity": 0.9690721649484536, "score": 193.0, "evalue": 1.79e-65, "description": "Internal stop codon at 15506. Nonsense mutation around 15506.", "psc": { "uniref90_id": "UniRef90_UPI00193A281B", "uniref50_id": "UniRef50_UPI00193A281B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A281B", "UniRef:UniRef50_UPI00193A281B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A281B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A281B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8782.442099999993, "isoelectric_point": 4.2705068588256845 }, "id": "LMJBGEANGM_69", "locus": "GCA013368015_00029" }, { "type": "cds", "sequence": "contig_1", "start": 15747, "stop": 15962, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A0D95F", "UniRef:UniRef90_UPI0034A0D95F" ], "nt": "ATGAATAAAAATATGTCAATAGTTAGCAAAAGTTTAAGAAATTTTTTTAATTCTTTACCTGAAAAAAAGTTTAATTCAAAAAAAATCACCGAATATAAAGAAAATCATTTACAAGCGATTGAAGAATTTGTTCAAATATACCAAGAAAAAATACTGTCAGATTTTGAATATTGTGTATATGATGCTTGTGATGTTTTGGCTGGGAAATCATCATGA", "aa": "MNKNMSIVSKSLRNFFNSLPEKKFNSKKITEYKENHLQAIEEFVQIYQEKILSDFEYCVYDACDVLAGKSS", "aa_hexdigest": "283f53e04c643fbf306fc41bfceab09f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 15747, "stop": 16220, "observations": { "mutation": [ 15960, 16047 ], "stop": [ 15960, 16047 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A0D95F", "query_cov": 1.0, "subject_cov": 0.45222929936305734, "identity": 0.972, "score": 135.0, "evalue": 2.76e-39, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNKNTLIVSKSLRNFFNSLPEKKFNSKKITEYKENHLQAIEEFVQIYQEKILSDFEYCVYDACDVLAGKSSWFNFEKDWPKEFCKDFFEYFKVLNELLANWSPKFISEKIVKPNKSILPFIIDAYNIRLLFSNEALIKEYAKYYLKQNKLVESGENV" }, "paralog": false, "identity": 0.9681528662420382, "score": 305.0, "evalue": 7.47e-109, "description": "Internal stop codon at 15960, 16047. Nonsense mutation around 15960, 16047.", "psc": { "uniref90_id": "UniRef90_UPI0034A0D95F", "uniref50_id": "UniRef50_UPI0034A0D95F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A0D95F", "UniRef:UniRef50_UPI0034A0D95F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A0D95F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A0D95F" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8333.416499999996, "isoelectric_point": 6.554070091247559 }, "id": "LMJBGEANGM_70", "locus": "GCA013368015_00030" }, { "type": "cds", "sequence": "contig_1", "start": 15878, "stop": 16123, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGCATCAATAATAAAAGGCAATATTGATTTGTTCGGCTTTACTATTTTCTCAGAGATAAATTTAGGGCTTCAATTAGCAAGTAATTCATTTAATACCTTAAAATATTCAAAAAAGTCTTTGCAAAATTCCTTTGGCCAATCTTTTTCAAAATTAAATCATGATGATTTCCCAGCCAAAACATCACAAGCATCATATACACAATATTCAAAATCTGACAGTATTTTTTCTTGGTATATTTGA", "aa": "MYASIIKGNIDLFGFTIFSEINLGLQLASNSFNTLKYSKKSLQNSFGQSFSKLNHDDFPAKTSQASYTQYSKSDSIFSWYI", "aa_hexdigest": "b5813725ace5671fef68d26d35cd2991", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9182.161699999999, "isoelectric_point": 8.964216041564942 }, "id": "LMJBGEANGM_71", "locus": "GCA013368015_00031" }, { "type": "cds", "sequence": "contig_1", "start": 16213, "stop": 16500, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939E4D6", "UniRef:UniRef90_UPI001939E4D6" ], "nt": "ATGTCTAGAAAAAATTTTGAAGATCAGTATTTTAATTTCGTTTCAAGAGAAAATGGTGAAAATAGAGTAAATGGATATTTTGTTGATATATTAGATTCTATAAAAGACACTGATTATGAGCAAGATGCATACATTCAATATAGAAGATTTATAGATAAAATTGAAAATACACCAATTACTTACACCATTTATGATGCTTTTATTGAGGATTTGCAACAGAATAAACTATTAGAAAACAAATTTATCGATTATTATAGAAAGGAAATAGATACCGACATATATGATTGA", "aa": "MSRKNFEDQYFNFVSRENGENRVNGYFVDILDSIKDTDYEQDAYIQYRRFIDKIENTPITYTIYDAFIEDLQQNKLLENKFIDYYRKEIDTDIYD", "aa_hexdigest": "219a8981aa0543d1170adbf90627fb39", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 16213, "stop": 16803, "observations": { "mutation": [ 16498, 16612 ], "stop": [ 16498, 16612 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939E4D6", "query_cov": 1.0, "subject_cov": 0.4377880184331797, "identity": 0.958, "score": 184.0, "evalue": 1.65e-57, "gene_start": 1, "gene_end": 95, "reference_start": 1, "reference_end": 95, "reference_sequence": "MSRKNFEDQYFNFVSRENGENRVNGYFVDILNSIKDTDYEQDAYIQYRRFIDKIENTPITCTIYDAFIEDLQQNKLLENKFIDYYKKKIDTDIYDWYDAKTKEIDFDNLLKTLINRYSYSIPQKEFELLFEKSWNNQEGYHEGFLIEPNIGFYDAFRNDLEYILDDLDYSQEDKKHFLNKYIETINNECERIINSIRREQKPNLLFIHPYDNDRGIY" }, "paralog": false, "identity": 0.9642857142857143, "score": 388.0, "evalue": 2.9e-140, "description": "Internal stop codon at 16498, 16612. Nonsense mutation around 16498, 16612.", "psc": { "uniref90_id": "UniRef90_UPI001939E4D6", "uniref50_id": "UniRef50_UPI001939E4D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939E4D6", "UniRef:UniRef50_UPI001939E4D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939E4D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939E4D6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11714.70489999999, "isoelectric_point": 4.385037422180175 }, "id": "LMJBGEANGM_72", "locus": "GCA013368015_00032" }, { "type": "cds", "sequence": "contig_1", "start": 16911, "stop": 17342, "strand": "+", "frame": 3, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F073", "UniRef:UniRef90_UPI0034A4F073" ], "nt": "ATGAAACATTTCAAAAAACTATTAATTACATTAACCGCTGCTGTTTCAACGACTGCGTTGCCATTAGTGGCAGCATCTTGCAACAATGAAAAATTAGCATTAGAAAATGGCAAAAAAGAATATGCAAATAACTTGCAATTATTAAAAAATGCTAAATCCCAATTGCAAAATTTGAATGTTGAATCAGAGCATCTAAAAAAAATTGATGAAAATATTGAAAAATCGATTAAATTGACTTCTAAAAATGCCACTTATAAAGAATATGCAAAAGCAAACACAATATTATCTGAAACCTTAAAACTGGCAACTCAATTAATTAACGATCATCCTAAACAAATTTATGAATATGCAAAGGGGATTCCTGAAATTCTTGAAAAATACGAATTGCATTTTTTAGAATTACCATTTTATTCAATGAAGCCAGAGCCATGA", "aa": "MKHFKKLLITLTAAVSTTALPLVAASCNNEKLALENGKKEYANNLQLLKNAKSQLQNLNVESEHLKKIDENIEKSIKLTSKNATYKEYAKANTILSETLKLATQLINDHPKQIYEYAKGIPEILEKYELHFLELPFYSMKPEP", "aa_hexdigest": "b734422a93d3fcf05334f223d114e439", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 16911, "stop": 17645, "observations": { "mutation": [ 17340 ], "stop": [ 17340 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A4F073", "query_cov": 1.0, "subject_cov": 0.5257352941176471, "identity": 0.9790000000000001, "score": 266.0, "evalue": 5.95e-88, "gene_start": 1, "gene_end": 143, "reference_start": 1, "reference_end": 143, "reference_sequence": "MKHFKKLLITLTAAVSMTALPLVAASCNNEKLALENGKKEYANNLQLLKNAKSQLQNLNIESEYLKKIDENIEKSIKLTSKNATYKEYAKANTILSETLKLATQLINDHPKQIYEYAKGIPEILEKYELHFLELPFYSMKPEPWIQPQNPEFDIKKAYEYSTGTPEIHGKEKMDLKLLLPSAINAEKQELEYLSELLFNFAMEINNANDKNNEKLNNLCSVISQKAGDINEYLNEPGTDYFFYLELKTIKEYTKNWFSQVKNEWKEFKKQNN" }, "paralog": false, "identity": 0.9836065573770492, "score": 477.0, "evalue": 9.14e-174, "description": "Internal stop codon at 17340. Nonsense mutation around 17340.", "psc": { "uniref90_id": "UniRef90_UPI0034A4F073", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI0034A4F073", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A4F073", "UniRef:UniRef50_UPI0034A4F073" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A4F073", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F073" ], "product": "variable surface lipoprotein" } }, "id": "LMJBGEANGM_73", "locus": "GCA013368015_00033" }, { "type": "cds", "sequence": "contig_1", "start": 17427, "stop": 17678, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCTTAAACTTTTATTACCAAGCGCAATAAATGCAGAAAAGCAAGAATTAGAATATTTATCTGAACTTCTATTCAATTTTGCTATGGAAATTAACAATGCTAACGATAAAAATAATGAAAAATTAAATAATTTATGCAGTGTAATTTCACAAAAAGCAGGAGATATAAACGAATATCTAAACGAGCCCGGAACTGATTATTTCTTTTATTTAGAATTGAAAACTATTAAAGAATATACAAAAAATTGA", "aa": "MDLKLLLPSAINAEKQELEYLSELLFNFAMEINNANDKNNEKLNNLCSVISQKAGDINEYLNEPGTDYFFYLELKTIKEYTKN", "aa_hexdigest": "da5bc2666fc2df6ee0fde0a01e3ce788", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9641.780199999994, "isoelectric_point": 4.481663703918457 }, "id": "LMJBGEANGM_74", "locus": "GCA013368015_00034" }, { "type": "cds", "sequence": "contig_1", "start": 18489, "stop": 18647, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGATAATCCCAAACCTACTAAACCAAATAATTCATATCCAAATGATTTGCCAAAGCCAAAATTGCCAAAGGTTCAAGAAGTAAAACCAGAAGATAGATTCGATACAAAACTTTTATATTCTTGTCCTGTTCCAGCAGTTTCTGACAAAAATTGA", "aa": "MYDNPKPTKPNNSYPNDLPKPKLPKVQEVKPEDRFDTKLLYSCPVPAVSDKN", "aa_hexdigest": "e177b39a77314ad0ae8d7561e741643c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5957.742900000002, "isoelectric_point": 8.78757266998291 }, "id": "LMJBGEANGM_75", "locus": "GCA013368015_00035" }, { "type": "cds", "sequence": "contig_1", "start": 18675, "stop": 18839, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAATATTCATAAATTAAAAGTCCTTGATTCTAATGGAAAAGAAACAAATGAATATGAATCAAAAGAACAAAAATTACAAAGATATGCCCAAGCAAAAACTGTGATTTTAAATTATTCTAATGCAGTAGAAGAAGCACGCAATAAATTGCTAGGTAATTGA", "aa": "MVNIHKLKVLDSNGKETNEYESKEQKLQRYAQAKTVILNYSNAVEEARNKLLGN", "aa_hexdigest": "58db96d79887c49f6869f174161c3644", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6221.963600000001, "isoelectric_point": 9.049443244934082 }, "id": "LMJBGEANGM_76", "locus": "GCA013368015_00036" }, { "type": "cds", "sequence": "contig_1", "start": 18849, "stop": 19448, "strand": "+", "frame": 3, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A3E62E", "UniRef:UniRef90_UPI0034A3E62E" ], "nt": "TTGTTTAAAAAATCAAAAGAAGAGTATGTTAGAAAAATTAAGAATAAAAAAGTATTACAAGATGACCCGAATTTTTTAATAAACTATGAAGGAAATTATCAATATTTTACCGAATCAAGAATTAAGGATAAAGATCCTCTATTATTATCCTTTTCAAATGAAACAATAAAAGAAATTAGAGAGCAAAACATTCAGACAGCAACTATTTCATTGGGACTTTCTCAATATATTGAATCATTTATGGAAATAAGAACAATTAGCGATTCTTTATTTAAAATAATTGACTTATTTACGTTTATGATAAACAATATAAATGACCCTGACAAAACCTTCTTGGAGCAACTACAAGCAATGGGTCCAGAGAAATTAGATAAATTAAAACATCTGATAAAAAATAATTTAACAATTGGCCAATTAATAAGCAATATAAAGATAGCCTTAGAATTAGACAATAAACTTTATGTATTTCCTGCTCCTATTGTCAAAAATTTTTATGATAAAGCAGGTCATTACAATAAAATCATTGATGCTTTAAATGAATTTTATAATCCCTTAGCATTTTTACTAGGTTTTATTACTGAAACGAATGATTTTTTTTGA", "aa": "MFKKSKEEYVRKIKNKKVLQDDPNFLINYEGNYQYFTESRIKDKDPLLLSFSNETIKEIREQNIQTATISLGLSQYIESFMEIRTISDSLFKIIDLFTFMINNINDPDKTFLEQLQAMGPEKLDKLKHLIKNNLTIGQLISNIKIALELDNKLYVFPAPIVKNFYDKAGHYNKIIDALNEFYNPLAFLLGFITETNDFF", "aa_hexdigest": "6935e2e913e7b8d14a9d61d15d9eb7ed", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 18549, "stop": 19652, "observations": { "mutation": [ 18645, 18666, 18837, 19446, 19485, 19599 ], "stop": [ 18645, 18666, 18837, 19446, 19485, 19599 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A3E62E", "query_cov": 1.0, "subject_cov": 0.4795180722891566, "identity": 0.965, "score": 381.0, "evalue": 2.44e-130, "gene_start": 1, "gene_end": 199, "reference_start": 149, "reference_end": 347, "reference_sequence": "MKKTRLLLTLTIIPTITAMPLISASCDWLYDKPKPTKPNNSYPNDLPKPKLPKVQEVKPEDRFDTKLLYSCPVPAVSDKNWDGPHQLWHYVVNIHKLKVLDSNGKETNEYESKEQKLQRYAQAKTVILNYSNAVEEARNKLLGNWWDDLFKKSKEEYVRKIKNKKVLEDDPNFLINYEGNYQYFTESRIKDKDPWLSSFSSETIKEIREQNIQTATISLGLSQYIESFMEIRTISDSLFKIIDLFTFMINNINDPDKTFLEQLQAMGPEKLDKLKHLIKNNLTIEQLIGNIKIALELDNKLYVFPAPIVKNFYDKAGHYNKIIDALNEFYNPLAFLLGFITETNDFFWNNPNPALKNKANWYDKYLFNKYEYGSNPNGFKIKTLKHYNAQSAFNALSKWLDSNKTSLGLKYSSIK" }, "paralog": false, "identity": 0.9591280653950953, "score": 697.0, "evalue": 1.25e-257, "description": "Internal stop codon at 18645, 18666, 18837, 19446, 19485, 19599. Nonsense mutation around 18645, 18666, 18837, 19446, 19485, 19599.", "psc": { "uniref90_id": "UniRef90_UPI0034A3E62E", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI0034A3E62E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A3E62E", "UniRef:UniRef50_UPI0034A3E62E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A3E62E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A3E62E" ], "product": "variable surface lipoprotein" } }, "id": "LMJBGEANGM_77", "locus": "GCA013368015_00037" }, { "type": "cds", "sequence": "contig_1", "start": 19773, "stop": 19988, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VBN4", "UniRef:UniRef90_UPI001588FCBF" ], "nt": "ATGAATAGTGGCACCTCTGGTGTAGAAACTCTTAAACAAGCATTAGGAACGTTGTTTGAGCAAATAAATCCATTTGTTTCACTGATAGCAGGTTATGCTGTTGTTATCTTTGCCTTAATTGCTATTGCTATGACATTCTTTGCGATTGCTTCATTGAAAACAGCCAAAACTCAAACTTCTAAGAAAGAAGCTAAATCTAGAATAGTGGGGATATGA", "aa": "MNSGTSGVETLKQALGTLFEQINPFVSLIAGYAVVIFALIAIAMTFFAIASLKTAKTQTSKKEAKSRIVGI", "aa_hexdigest": "f85a431a6ee3a2918905895972f9f87a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 19773, "stop": 20069, "observations": { "mutation": [ 19986 ], "stop": [ 19986 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001588FCBF", "query_cov": 1.0, "subject_cov": 0.7244897959183674, "identity": 1.0, "score": 128.0, "evalue": 3.69e-37, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNSGTSGVETLKQALGTLFEQINPFVSLIAGYAVVIFALIAIAMTFFAIASLKTAKTQTSKKEAKSRIVGIWLSFGFLIFFVVLIPVILKVATTFLAN" }, "paralog": false, "identity": 0.9897959183673469, "score": 180.0, "evalue": 3.32e-60, "description": "Internal stop codon at 19986. Nonsense mutation around 19986.", "psc": { "uniref90_id": "UniRef90_UPI001588FCBF", "uniref50_id": "UniRef50_A0A3Q9VBN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001588FCBF", "UniRef:UniRef50_A0A3Q9VBN4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3Q9VBN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VBN4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7507.806999999999, "isoelectric_point": 9.873542594909669 }, "id": "LMJBGEANGM_78", "locus": "GCA013368015_00038" }, { "type": "cds", "sequence": "contig_1", "start": 20138, "stop": 20341, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158C5032", "UniRef:UniRef90_UPI00158C5032" ], "nt": "ATGTTTATTTACCTTGTTGTTCTTTTTGCTCAGAAAACAAAAATAAAAATTCAATATGACAATAAATTAGAATATAAATCGGATGCAAAATACAAAAAAATAGATAAATTAACAAAGATATTTATATCAGTTTTTGCAATCTTGATAATTGCTGCATTAGTATTAATAACTGTTGGTATTGGTAATACTTATTTTATTAAATAG", "aa": "MFIYLVVLFAQKTKIKIQYDNKLEYKSDAKYKKIDKLTKIFISVFAILIIAALVLITVGIGNTYFIK", "aa_hexdigest": "8d52ab0a0ac9807bd3e4fcfc8f2319f2", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 20084, "stop": 20341, "observations": { "mutation": [ 20093 ], "stop": [ 20093 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158C5032", "query_cov": 1.0, "subject_cov": 0.788235294117647, "identity": 1.0, "score": 119.0, "evalue": 4.82e-34, "gene_start": 1, "gene_end": 67, "reference_start": 19, "reference_end": 85, "reference_sequence": "MFHWTFLVAIALSPVVLAMFIYLVVLFAQKTKIKIQYDNKLEYKSDAKYKKIDKLTKIFISVFAILIIAALVLITVGIGNTYFIK" }, "paralog": false, "identity": 0.9882352941176471, "score": 159.0, "evalue": 2.01e-52, "description": "Internal stop codon at 20093. Nonsense mutation around 20093.", "psc": { "uniref90_id": "UniRef90_UPI00158C5032", "uniref50_id": "UniRef50_UPI00158C5032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158C5032", "UniRef:UniRef50_UPI00158C5032" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00158C5032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158C5032" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7763.393099999996, "isoelectric_point": 9.77832279205322 }, "id": "LMJBGEANGM_79", "locus": "GCA013368015_00039" }, { "type": "cds", "sequence": "contig_1", "start": 20351, "stop": 20716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAATTTCAGCAATAACCATATTATTATCCCCAATTTTATATGCAATTTTTGCATTATTTTGGGGAATTTTTATTAGTTTTCCATTCCTTATATTGCAAGGTTTGTTCAAGGTAGCAAGATTTTTTGGTTTTGAATTAATTAACTATATTATTTTCAAAGTTAATCCAGGAGAATATACATTTAGTTTTTCAGATTTACCAGCGGCGTTTTTAGCGTTTTTACTATATGGATTAATTTTTACTATTATTTTTATAATTATTTCATTTGCTAAATATCAAATTGGCAAGAAAAACAAGAATCAAAACATAGAACATTTAAAATTCAAGAATGTTCTAAAACAATCATTTTCGGCTTTTCTATGA", "aa": "MLISAITILLSPILYAIFALFWGIFISFPFLILQGLFKVARFFGFELINYIIFKVNPGEYTFSFSDLPAAFLAFLLYGLIFTIIFIIISFAKYQIGKKNKNQNIEHLKFKNVLKQSFSAFL", "aa_hexdigest": "26ad390e7a4664c51e0b4aba1875e884", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13982.667099999977, "isoelectric_point": 9.744219017028808 }, "id": "LMJBGEANGM_80", "locus": "GCA013368015_00040" }, { "type": "cds", "sequence": "contig_1", "start": 21002, "stop": 21124, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGGGTCTTTATTAGTTAATATTGTTAAAAATTGTTTAAATCAATTTACTTTATTTATGATTTCACCATTAGTGGCAGCAAAATCAGTTGAAGATAATGGCAAAGCAATAATGAATTGA", "aa": "MLGSLLVNIVKNCLNQFTLFMISPLVAAKSVEDNGKAIMN", "aa_hexdigest": "0298745b73546a5ef264f2e242f7ee77", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4338.184, "isoelectric_point": 7.934269142150879 }, "id": "LMJBGEANGM_81", "locus": "GCA013368015_00041" }, { "type": "cds", "sequence": "contig_1", "start": 21206, "stop": 21961, "strand": "+", "frame": 2, "gene": null, "product": "Mbov_0396 family ICE element transmembrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TU60", "UniRef:UniRef90_UPI001939BB38" ], "nt": "TTGCTTCCGATAAAAGAAAAATTGAGGAACTTAGGAATCTTAATAATACCTGACCTAGTTATCTATATTGGGGCAGCATATGCTACAAAAGCCCTTAATAAGTTAATTGGCGATTTGCTAGATATTCAAGTACCTGACTTAGGATTCAAACAAGCCGGTAGATTTATAAGAACAGGCGCAGGAATTATTGCTGGCGCTACAACTGCCGGAGCAGCCGTGGCAGCAGGCAATGCAACATTAGGTTCAACTATTTCAAAGGGCGGTTTTTCAAACGCAATTGGAGGCTCTATCGGTAAGGCGGTTAATATATCGAGAATTAGACGAAATAAGGCATATGCAAAAGACTTGGGAATGAACAAAGAATTTATTAAATCTTCGTTTGAAAACGTGGGCAAAAATAAAGTAATAGAACAAAGAAAAATGCTTAATAATTTTAAAAATATGGCCACTGATGAAAAAGGAAACCTACACAGAGAATCATTATATGATTTGGACTCTTTGAAGCAATATAAGGCATCAGAATTACAAAAATTCAAAGATAAACAAGAATTATATGATGAATCATATAAGTTTTATAAGAAAACAAATACAGACAGTTATTTAGAACAAAAGCGGGCTGAATTAGAACATAACAGAACTCAAAATAAATATAAATACTCTATTTCTAAAGCAGAAAATCAATTAAAGCAATTAAATGATTTTGATTTAGAAAAAAACGATGTAAGAAAGGAAAATGAAAAAGATGCTTCAAAATAA", "aa": "MLPIKEKLRNLGILIIPDLVIYIGAAYATKALNKLIGDLLDIQVPDLGFKQAGRFIRTGAGIIAGATTAGAAVAAGNATLGSTISKGGFSNAIGGSIGKAVNISRIRRNKAYAKDLGMNKEFIKSSFENVGKNKVIEQRKMLNNFKNMATDEKGNLHRESLYDLDSLKQYKASELQKFKDKQELYDESYKFYKKTNTDSYLEQKRAELEHNRTQNKYKYSISKAENQLKQLNDFDLEKNDVRKENEKDASK", "aa_hexdigest": "bc1aa333fe07ca6491c91eabe8d9c892", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 20906, "stop": 21961, "observations": { "mutation": [ 20987, 21122 ], "stop": [ 20987, 21122 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939BB38", "query_cov": 1.0, "subject_cov": 0.46828358208955223, "identity": 0.988, "score": 429.0, "evalue": 1.58e-146, "gene_start": 1, "gene_end": 251, "reference_start": 286, "reference_end": 536, "reference_sequence": "MLISAITILLSPILYAIFALFWGIFISFPFLILQGLFKVARFFGFELINYIIFKVNPGEYTFSFSDLPAAFLAFLLYGLIFTIIFIIISFAKYQIGKKNKNQNVEHLKFKNVLKQSFSAFLWALLLPFLIFVSNIVLAIIFYYFGQALELQTNMKGDIISLLYVALNDNAEWAKESVAMNNFAPMPFSDYVAQLVSTSWGLGLMFIKNTIISWVCVIMLGSLLVNIVKNCLNQFTLFMISPLVAAKSVEDNGRAIMNWKHKFVESVISIFISLFALNFFAIFILILLPIKEKLSNLGILIIPDLVIYIGAAYATKALNKLIGDLLDIQVPDLGFKQAGRFIRTGAGIIAGATTAGAAVAAGNATLGSTISKGGFSNAIGGSIGKAVNISRIRRNKAYAKDLGMNKEFIKSSFENVGKNKVIEQRKMLNNFKNMATDEKGNLHRESLYDLDSLKQYKASELQKFKDKQELYDESYKFYKKTNTDSYLEQKRAELEHNKTQNKYKYSISKAENQLKQLNDFDLEKNDVRKENEKDASK" }, "paralog": false, "identity": 0.9829059829059829, "score": 637.0, "evalue": 6.14e-231, "description": "Internal stop codon at 20987, 21122. Nonsense mutation around 20987, 21122.", "psc": { "uniref90_id": "UniRef90_UPI001939BB38", "product": "Mbov_0396 family ICE element transmembrane protein", "uniref50_id": "UniRef50_A0A3T0TU60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939BB38", "UniRef:UniRef50_A0A3T0TU60" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3T0TU60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TU60" ], "product": "Transmembrane protein" } }, "id": "LMJBGEANGM_82", "locus": "GCA013368015_00042" }, { "type": "cds", "sequence": "contig_1", "start": 21953, "stop": 22105, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTGCTATTTTAAATTTTCAATTGAATTTATTAGTAAAACCGCTTAAAACAATTGCTAATGCTATTATTGCAAAAAATATTACAAAATCAAAAACTGTATATTTTCTTCATATTGTTAATTTTTTATCTGCTAATTTTTTATTTTGA", "aa": "MNIAILNFQLNLLVKPLKTIANAIIAKNITKSKTVYFLHIVNFLSANFLF", "aa_hexdigest": "4524df8624c32c332ed113865063a87e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5677.829699999998, "isoelectric_point": 10.301225852966304 }, "id": "LMJBGEANGM_83", "locus": "GCA013368015_00043" }, { "type": "cds", "sequence": "contig_1", "start": 22134, "stop": 22904, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATATTCAAAAAAACATAATTGTAGATTTTATGTCCTGGGTTTTAGAGCCTTTAAATATCTTGTGGGAGTAAGAAAATATGATATTAAATCAAAAAATAATACTAGTTTTTTAATACCATACAGTGAAATAATTAATGATGAAATTGTAATTAATAGAAAGAATAATTTTGGAATATTTAACCATTTCAGTGTTGTTAAGATAAAAGGATTTAATGTTTTTGTAAATTCGGAGCAAGATAGAGAAGCGGAGTTTTTGAGATTTACAAATCTCTTAAATTCTGTTGATGATAAAATAACTATTATTAAAACCTCTGCACCAATGGATTTAACTTCTAATATTAATGAAATTGATAAAAATTTAAATACCTCTTTTATTCAAAATAATAATCTAGCAAAAGAATATTGTTTATTGAATAAACAAGAATTAGAAACATTAAACGAAAAAGACAGCATTGATAATTATTATTTAGTTATTTATGGAAATTCTATTGAAGATTTAATGGCTAAAAGAACGAGAATAATTTTGCAATTAACGAATACAAAATTTAATCCTCAATTAGTAAAAGGAATTGATTTACTAAATGTATTAAATTTAATATTTAGAGTGCAATTAAATGACAAAAATTTATCCAATATTGAAAGGCCAGCATCATTACTTGCTCCTAAAAATGTAGAATTTTTTAAAAAGTATATGGTAGCTGATAATAAATATATTAGTTTTCAATCAATAAATGATTTCAATAACATTGAATTACAAGATGCTTGA", "aa": "MLYSKKHNCRFYVLGFRAFKYLVGVRKYDIKSKNNTSFLIPYSEIINDEIVINRKNNFGIFNHFSVVKIKGFNVFVNSEQDREAEFLRFTNLLNSVDDKITIIKTSAPMDLTSNINEIDKNLNTSFIQNNNLAKEYCLLNKQELETLNEKDSIDNYYLVIYGNSIEDLMAKRTRIILQLTNTKFNPQLVKGIDLLNVLNLIFRVQLNDKNLSNIERPASLLAPKNVEFFKKYMVADNKYISFQSINDFNNIELQDA", "aa_hexdigest": "f2641a7b9cb0c5c5e5d169fe468517a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29885.927300000047, "isoelectric_point": 8.838825035095216 }, "id": "LMJBGEANGM_84", "locus": "GCA013368015_00044" }, { "type": "cds", "sequence": "contig_1", "start": 22968, "stop": 23330, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAAAGATATTAAACAAAGCATCAGCAATGGTGGAAATAAACAACAATAGCAAATCAAAATTTAAAACAGTTAAATCAAAGGTTGAAATGGAAGCAATTGAAAATCTTATTTATTTGGCAAATACAAAAAATATGAATATTTTTAATAGCAATTTTATTTTTGTAAATCAGGGCAATAATTTAGACGAAATTAATGCATTAGAAACCCAAAATCAATTAAACGCTTCTGAAATATCAGCAAACATAAACCCTTTATTATTTAAACAAAAACAAGCATATGCAAGCGCTTGTTTAATAAATGGTGATAATATTGGGGATGATTTAGAAATGGTTTCAGAAAATATTTCAAGAGGTTGA", "aa": "MKEKILNKASAMVEINNNSKSKFKTVKSKVEMEAIENLIYLANTKNMNIFNSNFIFVNQGNNLDEINALETQNQLNASEISANINPLLFKQKQAYASACLINGDNIGDDLEMVSENISRG", "aa_hexdigest": "bc22b719c9bbefc410a5a925c83d7403", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13389.031099999984, "isoelectric_point": 5.141848564147948 }, "id": "LMJBGEANGM_85", "locus": "GCA013368015_00045" }, { "type": "cds", "sequence": "contig_1", "start": 23385, "stop": 23807, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF01580.24", "PFAM:PF10412.15" ], "nt": "ATGACAACTGATTCACCTTTATTTTTGGATATAAAAACAAAAAATTCGCAAAGAGTTTATTCGAATATGCTAATTACTGGAACACCAGGTTCAGGCAAATCGACACTTATGAAAAAAATGGTATTGCATTATTTAATTTATGGCACTGAAATCATTATTTTAGACCCTCAAAGGGAATATACAAAATTTGCAAAATTATTTGATGGCGAAGTTTTTGAAATGGGAACAGGAATTAACACGTTAATTAATCCTCTTCAACTAGATTTGCAATTTAATGAACAAGTAGAAATTAAAAATCAAAATAAAATTATAATATCTTCAAATATTAGAAAAGTTCAAAAATTCCTTAAAATAATGTGTAATTTTAATGATATAGAAGTTAGATTCATCGGAAAATCCTTGTCAGAGTTATATAATAAATGA", "aa": "MTTDSPLFLDIKTKNSQRVYSNMLITGTPGSGKSTLMKKMVLHYLIYGTEIIILDPQREYTKFAKLFDGEVFEMGTGINTLINPLQLDLQFNEQVEIKNQNKIIISSNIRKVQKFLKIMCNFNDIEVRFIGKSLSELYNK", "aa_hexdigest": "e3989a6668f0cbfacc9424949fa9378b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 75, "aa_cov": 0.5357142857142857, "hmm_cov": 0.8933333333333333, "evalue": 3.99253410252767e-10, "score": 34.75914001464844, "start": 14, "stop": 88 }, { "id": "PF01935.23", "name": "DUF87", "length": 73, "aa_cov": 0.5071428571428571, "hmm_cov": 1.0, "evalue": 4.279606381633849e-10, "score": 35.580665588378906, "start": 20, "stop": 90 }, { "id": "PF01580.24", "name": "FtsK_SpoIIIE", "length": 44, "aa_cov": 0.3142857142857143, "hmm_cov": 0.8863636363636364, "evalue": 3.3152040895076716e-07, "score": 25.486909866333008, "start": 3, "stop": 46 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 69, "aa_cov": 0.4857142857142857, "hmm_cov": 0.855072463768116, "evalue": 2.3515375647940084e-06, "score": 22.206382751464844, "start": 18, "stop": 85 } ], "seq_stats": { "molecular_weight": 16146.709699999992, "isoelectric_point": 9.162069511413574 }, "id": "LMJBGEANGM_86", "locus": "GCA013368015_00046" }, { "type": "cds", "sequence": "contig_1", "start": 24288, "stop": 24605, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGGAACGCAAAATTTTATGGATTTTAATCAAAATCAATTAGTTCAAACTAAATCTGCGGGGATTCTAGAAAATATGCAATATTCAATAACTCTAAGATCTAATTATCAAGATATAGAAGCATTAAAAACTCTATTCGAAAATAGAATGCCTTTAACAGAACAAGAATTGCAATTTTTATCTTCTGCTCCGATTGGGGGTGGAATATTATCAATCGATAACACAACAAGATTTCAATTCGAATCTTATTACAATGAATTAGAAAGAAAATTATTATTTAACAATGGCGATTTAAAAGCAATTGCAAATAATTAA", "aa": "MLGTQNFMDFNQNQLVQTKSAGILENMQYSITLRSNYQDIEALKTLFENRMPLTEQELQFLSSAPIGGGILSIDNTTRFQFESYYNELERKLLFNNGDLKAIANN", "aa_hexdigest": "0bf6a26f2302f1709a5f7afb74295a6c", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12063.43299999998, "isoelectric_point": 4.644507408142091 }, "id": "LMJBGEANGM_87", "locus": "GCA013368015_00047" }, { "type": "cds", "sequence": "contig_1", "start": 25269, "stop": 25496, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGCCTAAGTCACGCCAAAAAGCATTTGAATACTTTAAAAAAGAACTTTCTAAATTTATAGAAAGAGGAAAAAATGTTCAATTAATTACAGATTATAATCATGACAAAATTGATGATTATAAATTATTAGAGCCTGAATATGAGCAATTTAAAACTAACAGGCAATATTTTAAAGAGTGGGCAAAAAAACTAGATTTATCATTTTCTTATGAAAATTTCACTTGA", "aa": "MRPKSRQKAFEYFKKELSKFIERGKNVQLITDYNHDKIDDYKLLEPEYEQFKTNRQYFKEWAKKLDLSFSYENFT", "aa_hexdigest": "39172e7ed87f644ce54408ba8e3092ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9384.510699999997, "isoelectric_point": 9.09785900115967 }, "id": "LMJBGEANGM_88", "locus": "GCA013368015_00048" }, { "type": "cds", "sequence": "contig_1", "start": 25545, "stop": 26120, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATACATCAAATGATGAAAAATTTATATTGATTGAAAATCAGGGTTTCGATTTTAAAAAATCTAATAAATTAGAAAAATTAGGAATAATAAATAAATTAAGGTTATCAGAAACTCAAAAATATTTAGCATTCGATCAAATAAATAACAATAATCCTACCGGAATGGCAAATGATAAGCCCGAAAGAATAGCAAGCGATAAAAAGATAGGTCTTGAAGGACAAAATTATTACCTGGGAACTTATAAATTACATGCTCCTGCTGTCTTTTCTTTTACGGCGCCTAATGACAATTATGTTGTTAAGGTAAATGGCCATAAAGTTGATTTAATTAATAACAAATTCAATATTAAACTTGCAGATAAAAGAGAGGATGCAAGCGATGAACAAAAAATATACAATCAAGATTCTGATTCTTCTAAACCACAAGATGAAAAGAATTCGCATAAAAAAAATGAATATACAATTACTGTTGAAGAATATAAAAATATCAATGATCCATCAACAAAGACTATAACATATACCAAAAAATACATTATTGATTCAAAAAGTGGAAGTTTAGATTTTAAATGA", "aa": "MVNTSNDEKFILIENQGFDFKKSNKLEKLGIINKLRLSETQKYLAFDQINNNNPTGMANDKPERIASDKKIGLEGQNYYLGTYKLHAPAVFSFTAPNDNYVVKVNGHKVDLINNKFNIKLADKREDASDEQKIYNQDSDSSKPQDEKNSHKKNEYTITVEEYKNINDPSTKTITYTKKYIIDSKSGSLDFK", "aa_hexdigest": "9d2d1c638633bcf98ee6f05eee31bef6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21916.206900000005, "isoelectric_point": 8.462974357604981 }, "id": "LMJBGEANGM_89", "locus": "GCA013368015_00049" }, { "type": "cds", "sequence": "contig_1", "start": 26391, "stop": 26555, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCATTCTTTGCATACACATTGTTTAATTCAAAAGGCGATAAAATACGAGATAAGGGCTTTATTGCAGAGGCTACCGTAATCTCAAAAGCTGCTCTAAAAATGCTTGCCGGTAAAACAGAAGAGTATTACATAATTAAAATTGATAATAAATCAAAAAAATGA", "aa": "MPFFAYTLFNSKGDKIRDKGFIAEATVISKAALKMLAGKTEEYYIIKIDNKSKK", "aa_hexdigest": "ecbbc18e91ff3298aeef395e4e573f08", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6134.169699999999, "isoelectric_point": 9.647451972961427 }, "id": "LMJBGEANGM_90", "locus": "GCA013368015_00050" }, { "type": "cds", "sequence": "contig_1", "start": 26601, "stop": 26750, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATGAAGGAACATATCTCTTATATTCTAGAAGTTCAAAAGATTCAATTAATAACTTCAAGATTTTAAAAATTAGCAATGAACAAACAATAAATAAGAAAATGTTTTCAGAAATAAACAAAATTGATTCAATTAAGAATTTATGA", "aa": "MSNEGTYLLYSRSSKDSINNFKILKISNEQTINKKMFSEINKIDSIKNL", "aa_hexdigest": "5497e751c08adb8b562fa440b50b585f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5699.468399999999, "isoelectric_point": 9.456109809875489 }, "id": "LMJBGEANGM_91", "locus": "GCA013368015_00051" }, { "type": "cds", "sequence": "contig_1", "start": 26916, "stop": 27194, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTTTCATCAGAATCTAATAAATTCAAGGATAAAACTGAATTTTTAGAATATTTAAAACAAAATAATATCGATATTATTGATAAATATGGAAAATTTGAAAATAAAGAATTAGTAGGAATTGAAAAATATAATTTCATAGACAACAATCAACTAGAAATTACTTATAATCTAAAAACTGCTGATGACAGGTATAGATTAACGGTCTTAAAGCAAACTTATTTAATTAATTTTATTAATGATAAAACAAATAAAACCGAAATATTTATTCAATGA", "aa": "MDFSSESNKFKDKTEFLEYLKQNNIDIIDKYGKFENKELVGIEKYNFIDNNQLEITYNLKTADDRYRLTVLKQTYLINFINDKTNKTEIFIQ", "aa_hexdigest": "924f61156c69b3bf1866cc32b9e255c7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11119.401199999986, "isoelectric_point": 5.132242774963378 }, "id": "LMJBGEANGM_92", "locus": "GCA013368015_00052" }, { "type": "cds", "sequence": "contig_1", "start": 27363, "stop": 27857, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAGAATATGCTGATAAATATTATTTGACTAATAACCAATTCAAAATTGAGGCAATTTTTAAACCAGTTGATTTATTTGATGATAGATTTGAACCTAAAATGATTAATTTAAAAGGTGAAACAAGAGAAGAACAAATAAAACAAATAATTACTCTTGAAATAAAGAAACAAATTATTCAATTTTTTAAGAAATTTGACATTGATGTATCTGAAAACGACATAAAAATTCAAGAAATTACAAGTGAAATTGTACAAAAATTGAAAAATGTTTATAAAACAAAAGAAATTGCGTTAAACAATGCAACAAAACTAAATGTTGATATAAACAATAAAACACATAATATAAAAGCAAAAACCACATTTGAAATTATTAATTTTTCAGATACTTATGTTTCGAATATTATTGATTTAAGCAAATTAAAGCTTGACCCTATTGATATAAATTATGATGTTTCTTTTTCAAATAATAAAGAATATCAAACAAAATGA", "aa": "MKKEYADKYYLTNNQFKIEAIFKPVDLFDDRFEPKMINLKGETREEQIKQIITLEIKKQIIQFFKKFDIDVSENDIKIQEITSEIVQKLKNVYKTKEIALNNATKLNVDINNKTHNIKAKTTFEIINFSDTYVSNIIDLSKLKLDPIDINYDVSFSNNKEYQTK", "aa_hexdigest": "5eed6a4870df6b4dd901219c87b3a5b2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19427.057499999995, "isoelectric_point": 7.7471204757690435 }, "id": "LMJBGEANGM_93", "locus": "GCA013368015_00053" }, { "type": "cds", "sequence": "contig_1", "start": 27864, "stop": 28172, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTAGTTAAAATAGAAATTCAAAAGCAATTAAATTCCTTAAATCCTGAATTAGTTTTGACTAAAAATGTAATTTATAATGAAGAGGAATTTGAAAACGTAATTACTAATTTATTTTATAAAGACATTATTTCAACATTGAATATATTTCCTAACCATCCAATGATAAAAAATTCTACTTCAATAGAAATTACTAATGGCCATAAAACAACACAAAAATTTAATTTTGGTGATTTAGAACTAGATGATTTAAACATTAAATCAAAAAATTACACCGAAATTAAGAAATTGATAATTCAATGA", "aa": "MANLVKIEIQKQLNSLNPELVLTKNVIYNEEEFENVITNLFYKDIISTLNIFPNHPMIKNSTSIEITNGHKTTQKFNFGDLELDDLNIKSKNYTEIKKLIIQ", "aa_hexdigest": "e8505a6b83136b32d78528f19867ab59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11868.506399999984, "isoelectric_point": 5.6337900161743155 }, "id": "LMJBGEANGM_94", "locus": "GCA013368015_00054" }, { "type": "cds", "sequence": "contig_1", "start": 28197, "stop": 28406, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATATAACCAATTTAGCATACGAAAAAGATTATTTAATCGAAGAACTTAACAACATAGATTACATGAAGACCTTAATATCTAAAAAAGGTAAGAATGAACTAACATTGACCATAAAACCAGTTAATAAAAGTTATGAAGGCGAAACAAAATTAAAAGTTATTAATGATTTTGATGGAAATTTATCGGAAGAAGATAAAAAAACCTGA", "aa": "MYITNLAYEKDYLIEELNNIDYMKTLISKKGKNELTLTIKPVNKSYEGETKLKVINDFDGNLSEEDKKT", "aa_hexdigest": "d5d7c9a62ca3cc940617f4dbe65b4447", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8050.0803, "isoelectric_point": 5.070288276672362 }, "id": "LMJBGEANGM_95", "locus": "GCA013368015_00055" }, { "type": "cds", "sequence": "contig_1", "start": 28443, "stop": 28721, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTTTCATCAGAATCTAATAAATTTAAGGATAAAACCGAATTTTTAGAATATCTAAAACAAAATAATATCGATATTATTGATAAATATGGAAAATTTGAAAATAAAGAATTAGTAGGAATTGAAAAATATAATTTCATAGACAACAATCAACTAGAAATTACTTATAATCTAAAAACTGCTGATGACAGATATAGATTAACAGTCTTAAAGCAAACTTATTTAATTAATTTTATTAATGATAAAACAAATAAAACCGAAATATTTCTTCAATGA", "aa": "MDFSSESNKFKDKTEFLEYLKQNNIDIIDKYGKFENKELVGIEKYNFIDNNQLEITYNLKTADDRYRLTVLKQTYLINFINDKTNKTEIFLQ", "aa_hexdigest": "1dbffc7fc6fbeb6b6f7dfbc981c93c7a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11119.401199999986, "isoelectric_point": 5.132242774963378 }, "id": "LMJBGEANGM_96", "locus": "GCA013368015_00056" }, { "type": "cds", "sequence": "contig_1", "start": 28920, "stop": 29384, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAATAACCAATTCAAAATTGAGGCAATTTTTAAACCAGTTGATTTATTTGATGATAGATTTGAACCTAAAATGATTAATTTAAAAGGTGAAACAAGAGAAGAACAAATAAAACAAATAATTACTCTTGAAATAAAGAAACAAATTATTCAATTTTTTAAGAAATTTGACATTGACATATCGGAAAATGACATAAAAATTCAAGAAATTACAAGTGAAATTGTACAAAAATTGAAAAATGTTTATAAAACAAAAGAAATTGCGTTAAACAATGCAACAAAACTAAATGTTGATATAAATAATAAGAAACATAATATAAAAGCAAAAACCACATTTGAAATTATTAATTTCTCAGATACTTATGTTTCGAATATTATTGATTTAAGCAAATTAAAGCTTGACCCTATTGATATAAATTATGATGTTTCTTTTTCAAATGATAAAGAATATCAAACAAAATGA", "aa": "MTNNQFKIEAIFKPVDLFDDRFEPKMINLKGETREEQIKQIITLEIKKQIIQFFKKFDIDISENDIKIQEITSEIVQKLKNVYKTKEIALNNATKLNVDINNKKHNIKAKTTFEIINFSDTYVSNIIDLSKLKLDPIDINYDVSFSNDKEYQTK", "aa_hexdigest": "56aefd4ded4be61985da6f758a8bf6f1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18166.66389999999, "isoelectric_point": 6.617559242248535 }, "id": "LMJBGEANGM_97", "locus": "GCA013368015_00057" }, { "type": "cds", "sequence": "contig_1", "start": 29391, "stop": 29699, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTAGTTAAAATAGAAATTCAAAAGCAATTAAATTCCTTAAATCCTGAATTAGTTTTGACTAAAAATGTAATTTATAATGAACAAGAATTTGAAAACATAATTACTAATTTATTTTATAAAGACATTATTTCAACATTGAATATATTTTCTAACCATCCAATGATAAAAAATTCTACTTCAATAGAAATTACTAATGGCCATAAAACAGCACAAAAATTTAATTTTGGTGATTTAGAACTAGATGATTTAAACATTAAATCAAAAAATTACACAGAAATTAAAAAATTAATAATTCAATGA", "aa": "MANLVKIEIQKQLNSLNPELVLTKNVIYNEQEFENIITNLFYKDIISTLNIFSNHPMIKNSTSIEITNGHKTAQKFNFGDLELDDLNIKSKNYTEIKKLIIQ", "aa_hexdigest": "62869c821c25db2f91accb053d10dae1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11841.484299999984, "isoelectric_point": 6.079748725891112 }, "id": "LMJBGEANGM_98", "locus": "GCA013368015_00058" }, { "type": "cds", "sequence": "contig_1", "start": 29724, "stop": 29933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATATAACCAATTTAGCATACGAAAAAGATTATTTAATCGAAGAACTTAACAACATAGATTACATGAAGACCTTAATATCTAAAAAAGGTAAGAATGAACTAACATTGACCATAAAACCTTCTAATGATAGTTGCGAAGGTGAAACAAAATTAAAAGTTATTAATGATTTTGATGGCGACTTATCTAAAACAGAAGATAAACGCTGA", "aa": "MYITNLAYEKDYLIEELNNIDYMKTLISKKGKNELTLTIKPSNDSCEGETKLKVINDFDGDLSKTEDKR", "aa_hexdigest": "18e8e5c1b1dcaf944e1c008cb9431816", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7992.967899999998, "isoelectric_point": 4.848388862609862 }, "id": "LMJBGEANGM_99", "locus": "GCA013368015_00059" }, { "type": "cds", "sequence": "contig_1", "start": 30327, "stop": 30797, "strand": "+", "frame": 3, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158F2D97", "UniRef:UniRef90_UPI00158F2D97" ], "nt": "TTGTTTAAAGATATTAAAAATAAGAATATTGGCTTTGAATTACGTAAAAAAGTAATCTCATTATATTTAAACGGGCTAAGTTATAGAAAAATAGCTAAAAATTATGGAATTAGCCACACAGAAATTTTTAAAATAGTCAAAAATCATTTTCCTCATTCTTTAAAAGAAGCAAAAATAATTAATTTTAAGGAATTTATAAACAAAAATGAGAATATTTATATTGCAGTAGATGATACTTATTTAAAAGTACGACAAGGCCAAAATGAAATAAACAAAATTAAAGCAAGAATAATTAATTTCTTTGCATTAGATAAAAATAAAAAGCCTTATTGCAAAAACCATTTGATTTATTTTTCGAGCCCAAATAGAAATATGAATTTAAATGATTTAGTAGAAAAAATTAATTCTGTAATTAGGAATAATTATTCAGAAGAAGCCAATATTATAATCACAGGAGATGGGGCAAAATGA", "aa": "MFKDIKNKNIGFELRKKVISLYLNGLSYRKIAKNYGISHTEIFKIVKNHFPHSLKEAKIINFKEFINKNENIYIAVDDTYLKVRQGQNEINKIKARIINFFALDKNKKPYCKNHLIYFSSPNRNMNLNDLVEKINSVIRNNYSEEANIIITGDGAK", "aa_hexdigest": "3f6b10273b7b1a440a216e8c9f36aa0c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 30120, "stop": 31100, "observations": { "mutation": [ 30204, 30219, 30795, 31011 ], "stop": [ 30204, 30219, 30795, 31011 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158F2D97", "query_cov": 1.0, "subject_cov": 0.40310077519379844, "identity": 0.9940000000000001, "score": 309.0, "evalue": 2.85e-103, "gene_start": 1, "gene_end": 156, "reference_start": 70, "reference_end": 225, "reference_sequence": "MEAIEIIKEKIINEDIEFFNSVARKNQGWRVKDWIYRKLTFEFGTIRVKIRKYVRKIDNKYENYNVCLSLFKDIKNKNIGFELRKKVISLYLNGLSYRKIAKNYGISHTEIFKIVKNHFPHSLKEAKIINFKEFINKNENIYIAVDDTYLKVRQGQNEINKIKARIINFFALDKNKKPYCKNHLIYFSSPNRNMNLNDLVEKINSVIRNNYSEEANIIITGDGAKWIRTLSKKLSARYILCKFHLHSKFQVIFNHSNHLKKELEKLEKITKFNLFNILYTYLKNHKYIEIFNLVSKHWDSIKKCLSEKRAKLLHDFAYYVHLNITGLSEIEECDKNYYGNIAESYVSHIIKSKTKKFYSICNIKTIIQRIINPYDYIKNNIEIHIID" }, "paralog": false, "identity": 0.9877300613496932, "score": 612.0, "evalue": 5.21e-225, "description": "Internal stop codon at 30204, 30219, 30795, 31011. Nonsense mutation around 30204, 30219, 30795, 31011.", "psc": { "uniref90_id": "UniRef90_UPI00158F2D97", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_UPI00158F2D97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158F2D97", "UniRef:UniRef50_UPI00158F2D97" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00158F2D97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158F2D97" ], "product": "Mbov_0401 family ICE element transposase-like protein" } }, "id": "LMJBGEANGM_100", "locus": "GCA013368015_00060" }, { "type": "cds", "sequence": "contig_1", "start": 31804, "stop": 31998, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTAAATACTTAATTAGTTCGATTGGTTTGGGGAATGAAAAATCTTTGTTATTTAATATCGATATAAGCAGTCTCTTGCCAGTACTTGTGGATATATTTCCTTCATTAATTAAATTAGAAAAATTGTTGCCTCTTTCTCTAGAAATGATTTTGTATGGTTTATTTCTATCAACTATGACTTTTTCATAA", "aa": "MLIKYLISSIGLGNEKSLLFNIDISSLLPVLVDIFPSLIKLEKLLPLSLEMILYGLFLSTMTFS", "aa_hexdigest": "f6c76e6f7f60a681007d520c78a72e5a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7160.661099999995, "isoelectric_point": 4.93575038909912 }, "id": "LMJBGEANGM_101", "locus": "GCA013368015_00061" }, { "type": "cds", "sequence": "contig_1", "start": 32016, "stop": 32231, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACCATGTTTCATCCAAAAGCAGTTTTCAATATATCGAATCACTTGATTATGAAATTCTTGCTCCAGATGGTACAATGTTTAAAAATTATAGAAATATATTAAAACCTCGTTCTTACCGTTATACTCTGGGTAAGGAATTGTTTGAATTTTATAATTCAAATAGATTTATTGAAATTCAACAAAAAACCTATAAAGACGGAACAAAATATTGA", "aa": "MDHVSSKSSFQYIESLDYEILAPDGTMFKNYRNILKPRSYRYTLGKELFEFYNSNRFIEIQQKTYKDGTKY", "aa_hexdigest": "39cdbfcde07a20d0abfc494e6ad26548", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8608.593599999995, "isoelectric_point": 8.859583854675293 }, "id": "LMJBGEANGM_102", "locus": "GCA013368015_00062" }, { "type": "cds", "sequence": "contig_1", "start": 32412, "stop": 32561, "strand": "-", "frame": 1, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025CFB7FC" ], "nt": "ATGATGAACGAAAGATTAAAAATGGCTCGCCAACTTTTAAAAGAAGGTGGTGTTATTTTTGTTTCAATTGATGACACTGAACAAGCTTATTTAAAAGTATTAATGGACGAAATTTTTGGGGAAGAAAATTTTGTATGTAATTTTGTGTGA", "aa": "MMNERLKMARQLLKEGGVIFVSIDDTEQAYLKVLMDEIFGEENFVCNFV", "aa_hexdigest": "e465411991872014d32a3498f5eee890", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0025CFB7FC", "query_cov": 0.8979591836734694, "subject_cov": 0.88, "identity": 0.682, "score": 63.2, "evalue": 2.44e-12, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0025CFB7FC" }, "pscc": { "uniref50_id": "UniRef50_UPI0025CFB7FC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0025CFB7FC" ], "product": "DNA methyltransferase" }, "genes": [], "id": "LMJBGEANGM_103", "locus": "GCA013368015_00063" }, { "type": "cds", "sequence": "contig_1", "start": 32701, "stop": 33063, "strand": "-", "frame": 3, "gene": null, "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)", "db_xrefs": [ "RefSeq:WP_041359577.1", "SO:0001217", "UniParc:UPI0001BC1FCC", "UniRef:UniRef100_D1J7F1", "UniRef:UniRef50_D1J7F1", "UniRef:UniRef90_D1J7F1" ], "nt": "ATGAAGGATTTAAAAAACTATATAGATGATATAGATAACTTGAATTCTTTTTTATTAAATCAAGACCAAAAGAAATTAATAAAAGAAATTTTAGTTAACACAAACAATTCAAATAAAGAAAACTTGGACAATGTTTTCCAACTTTTGATTCAAAGAGTTAAAACAGGCTTTGTTTTTGATGTTGCGCCTAGTGTTGATGCAAAACAAATTGCAATATTAAAAAAACAAGAAAAATTATCATTTACAAATGATTTATTGAATACTAATAAAAACACTTTAATTATTGGTGAAAACTATGATGCATTAAAAAACCTACTTGTAATAGAAAGACAAAAAGAACAAATAGGGGGGGGGGGATTATAA", "aa": "MKDLKNYIDDIDNLNSFLLNQDQKKLIKEILVNTNNSNKENLDNVFQLLIQRVKTGFVFDVAPSVDAKQIAILKKQEKLSFTNDLLNTNKNTLIIGENYDALKNLLVIERQKEQIGGGGL", "aa_hexdigest": "19c533d4eea4383d6d12bbf2107f220a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC1FCC", "ncbi_nrp_id": "WP_041359577.1", "uniref100_id": "UniRef100_D1J7F1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FCC", "RefSeq:WP_041359577.1", "UniRef:UniRef100_D1J7F1" ] }, "ips": { "uniref100_id": "UniRef100_D1J7F1", "uniref90_id": "UniRef90_D1J7F1" }, "psc": { "uniref90_id": "UniRef90_D1J7F1", "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)", "uniref50_id": "UniRef50_D1J7F1" }, "pscc": { "uniref50_id": "UniRef50_D1J7F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7F1" ], "product": "Pseudogen of Type III restriction modification system: methylase (Part 1)" }, "genes": [], "id": "LMJBGEANGM_104", "locus": "GCA013368015_00064" }, { "type": "cds", "sequence": "contig_1", "start": 33371, "stop": 33460, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGCAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGCCAAACACCAATAAAAAAAGCAATTTGA", "aa": "MKSKEIDAKQAKQERAEKLFGQTPIKKAI", "aa_hexdigest": "7cc687f2a292349fe2a581bf1a29485e", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3315.883600000001, "isoelectric_point": 9.873542594909669 }, "id": "LMJBGEANGM_105", "locus": "GCA013368015_00065" }, { "type": "cds", "sequence": "contig_1", "start": 33494, "stop": 33913, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FC234F" ], "nt": "ATGGCTGGGCTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTGCATTCAATTACGAAATTAGCTGAAATTACTCCTAATAGTATTGTATCGACAGCTTCGGCAGTATTTGGTCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTGCCTGTTGGAGCTTCAATTTATTACACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACGATGTTTTGGTTTACGGTTGCACTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGA", "aa": "MAGLYGFIDQIFILNLVPKNRNIFGNNESEIVQFLNPALHSGGFFSDYKIMIEQYNLHSITKLAEITPNSIVSTASAVFGPLILFSNAIVFLVPVGASIYYTKCVSKQLKRAGQDLWATMFWFTVALSLFATLVSFVFI", "aa_hexdigest": "18c33e03fb1f6f68af633214941e2da1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002FC234F", "query_cov": 0.9856115107913669, "subject_cov": 0.8154761904761905, "identity": 0.569, "score": 152.0, "evalue": 1.72e-44, "valid": false, "uniref50_id": "UniRef50_UPI0002FC234F" }, "pscc": { "uniref50_id": "UniRef50_UPI0002FC234F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FC234F" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15496.946199999986, "isoelectric_point": 7.86406307220459 }, "id": "LMJBGEANGM_106", "locus": "GCA013368015_00066" }, { "type": "cds", "sequence": "contig_1", "start": 34754, "stop": 34906, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGTTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTCGCCTCAATATTTGAAAACTTTATTTGCTTTGTAATAATTGTTCCTATTGGATATGGAATAGCTTGA", "aa": "MSLRIIAVSFVVVSLLAFQGSNDIAKSIFASIFENFICFVIIVPIGYGIA", "aa_hexdigest": "7e3886a0f6f4f97d10d162cf23a13065", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5395.421199999997, "isoelectric_point": 5.820676612854004 }, "id": "LMJBGEANGM_107", "locus": "GCA013368015_00067" }, { "type": "cds", "sequence": "contig_1", "start": 35260, "stop": 35721, "strand": "-", "frame": 3, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "RefSeq:WP_175936633.1", "SO:0001217", "UniParc:UPI00158D0BD4", "UniRef:UniRef100_UPI00158D0BD4", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_D1J7F6" ], "nt": "ATGGAAAATAATTCAAAAAACTTGAAAACCAAAGAAAATGCAAAACTTCTAATAATATTTTCAGCAATCTTATTAGGAATAGATGTTATTTATATTATTTGCGGTTCATTTGTACCAGTATTTGGATTATTATTAGCAATAGCAAGCATCGTCTTTTTTATTCTAACAGTTGTTTATGGATTTAAAACAGGATCTCGCGGTTTAAGTAAAAATGAAATAACAACAAGAAAATTATCAATTTCATTAGTTGCCTTGTTTATAATTTCATTTGTCTTAGCAATTGTTGCTACAGCAATGTTTTTATCAGGCGCTGCTAAAACTGCTATAGCAATATATGGACTTTCAGTAATTGCTAATCTTGCACAATTAGGTGTTTTAATTGCATTATTATGTTTTGCAGTAAAAACTTATAATAGTAAAAACAATTCACACCCAGCCCAACAACAAAATTTAAGACAATAA", "aa": "MENNSKNLKTKENAKLLIIFSAILLGIDVIYIICGSFVPVFGLLLAIASIVFFILTVVYGFKTGSRGLSKNEITTRKLSISLVALFIISFVLAIVATAMFLSGAAKTAIAIYGLSVIANLAQLGVLIALLCFAVKTYNSKNNSHPAQQQNLRQ", "aa_hexdigest": "c175a1454ad1722f5d9cad1de3a27c01", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D0BD4", "ncbi_nrp_id": "WP_175936633.1", "uniref100_id": "UniRef100_UPI00158D0BD4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D0BD4", "RefSeq:WP_175936633.1", "UniRef:UniRef100_UPI00158D0BD4" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D0BD4", "uniref90_id": "UniRef90_D1J7F6" }, "psc": { "uniref90_id": "UniRef90_D1J7F6", "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_108", "locus": "GCA013368015_00068" }, { "type": "cds", "sequence": "contig_1", "start": 35904, "stop": 36434, "strand": "-", "frame": 1, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_UPI00093E8FF7" ], "nt": "GTGAATAATAACTTAAAAACTAAAGAAAATGCAAAACTTCTAATAATATTTTCATCAATCTTATTAGGAATAGATGTTATTTATATTATTTGTGGTTCGCTTGTACCAGTATTTGGATTATTATTAGCAATAGCAAGCATTGTGTTTTTTATTTTAACAGTTGTGTATGGATTTAAAACAGGATCACGTGAACCAAAAAATAGTAATCGAATGATAATAAGAAAATTATCCATTGCATTAATAGCATTGTTTGCAGCAGCAATTGCAATAGTTATTGTTGCAATAATAATAGCAATATCTTTATCACTTGGCGCATATACTTATGAAGACCCAAATGAATATAATCCATTTTTATTAGTGTCATCAACATCTGCACTATATATTATTGGTTTAATTTTGTCCCTTGCAGAATTAGGCGTTTTAATTGCATTATTATGTTTTGCAGTTAAGGTCTATTGTGCTAAAAATAACAATAATGTTAATAATGAAACAAACAACTATGGTGGCGACTCTCAAAAGCCTCAAAATTGA", "aa": "MNNNLKTKENAKLLIIFSSILLGIDVIYIICGSLVPVFGLLLAIASIVFFILTVVYGFKTGSREPKNSNRMIIRKLSIALIALFAAAIAIVIVAIIIAISLSLGAYTYEDPNEYNPFLLVSSTSALYIIGLILSLAELGVLIALLCFAVKVYCAKNNNNVNNETNNYGGDSQKPQN", "aa_hexdigest": "385ab4f214a462db1d888382dccfe2cc", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00093E8FF7", "query_cov": 1.0056818181818181, "subject_cov": 0.9619565217391305, "identity": 0.9209999999999999, "score": 269.0, "evalue": 7.16e-90, "valid": true, "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_109", "locus": "GCA013368015_00069" }, { "type": "cds", "sequence": "contig_1", "start": 36559, "stop": 37443, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "TTGTCTGAAACATTAAAAAAGAGAGTGAAAGGTCAAGATCAAGCAATTGAACTAATCTCTGATGCAATCAAACGTTCTAAAGCAAACATCAATGACCCAAATAGACCAATTGGTTCGTTCCTATTTCTAGGGCCAACTGGTGTTGGTAAAACTGAACTTGCAAAAAGTTTAGCAGAAGCTTTATTTGATGATGAAAATCACATTGTAAGATTAGACATGTCTGAATATATGGAAAAACACAGCGTGTCAAAATTAATTGGATCGCCTCCAGGTTATATTGGTTATGATAACGGGGGACAACTAACTGAAAAAATTAGACAAAATCCTTACTCAATAGTTTTATTTGATGAAATTGAAAAAGCAAATTCAGATGTTTTAAATATATTGTTACAAATATTGGACAATGGTACCTTAACAGATAGTACAGGAAGGGTAGTAAACTTTAAAAACACAATAATTATCATGACTTCAAACATTGGAAGTAGTGAAATTATAAATAAGAGTTTAACCATTGATGGGGTAAGGGCATTATTATTAAGAAGTTTTAGACCAGAATTTATTAATAGAATCGATGAAATAGTGCCATTCAACCCATTAAGTCCTGCTGTTGTTTGTGACATTGTAAAACTAGAATTATCAAAATTAAAGAAAAGAGTTATTAATAACCATAATTTAGTAATTAATTTTGATGACAAAATAGTTAAATTCGTTGCTGATAACGCCTACGATTCAGCCTTTGGCGCTCGTCCAATTAAAAGATATATTCAAAAAAACATTGAAAACAAATTAGCAATGGAAATCATAAAAGGAAATTTAGTTGAAGGTGATGTTGCCTTATTAACAGTTTCAAATAATAATGTTATTGAATTACAAACAAATAATTAA", "aa": "MSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN", "aa_hexdigest": "28a3a307c9f5b3ecd2df9033bc508995", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPN6", "query_cov": 0.9829931972789115, "subject_cov": 0.8376811594202899, "identity": 0.5539999999999999, "score": 315.0, "evalue": 1.22e-103, "valid": false, "product": "ATP-dependent Clp protease ATP-binding subunit, 2", "uniref50_id": "UniRef50_A0A356L436" }, "genes": [ "clpA", "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 36559, "stop": 37743, "observations": { "mutation": [ 37500 ], "stop": [ 37500 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.4106145251396648, "identity": 0.997, "score": 563.0, "evalue": 2.76e-196, "gene_start": 1, "gene_end": 294, "reference_start": 423, "reference_end": 716, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.9974619289340102, "score": 754.0, "evalue": 1.24e-273, "description": "Internal stop codon at 37500. Nonsense mutation around 37500.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "LMJBGEANGM_110", "locus": "GCA013368015_00070" }, { "type": "cds", "sequence": "contig_1", "start": 37498, "stop": 38709, "strand": "-", "frame": 3, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "ATGGAAGCAAGTTATACACCTTATGAACAAGAAAAAGATCCCTTGAAGGAGTTTGGTCGAAACTTAACTGAGTTAGCTACGCAGAACAAACTAGAACCTGTTATAAACCGTGATGAAGAAATAAGATCACTAGTTAGAATTTTGTCAAGAAAAACTAAAAATAATCCCGTTTTAGTTGGCGAACCCGGCGTAGGTAAAACAGCAATTGTTGAAGGCCTAGCAAGGAAAATTGTTGAAAATCAAGTTCCAGAAAACCTAAAAGGTAAACAATTAATTGAAATTGATTTACCAGCATTGGTTGCAGGAACACAATATCGTGGTCAATTTGAAGAAAGACTAAAAAAAGTATTGAAAAAGATTGACGAAGCAAATGGTGAAATCATTCTTTTCATTGATGAAATTCATATGATAATTGGCGCCGGAGGAACTGGCGATAGCAATATGGATGCGGCGAATATATTGAAACCTATGATGGCAAGAGGTCAATTGCATCTAATTGGGGCAACAACATTGGATGAATATAGAAAATATATTGAAAAAGATCCTGCCCTTGAAAGAAGAATGCAAAAAATTTTAATTTCAGAACCTTCAATTGAAGATACTATAACTATATTGCGTGGTATAAAAGAAAGATTTGAAGCATATCATGAAGTTAAAATTGATGACTCTGCTATAGTTTCGGCAGCAAATTTATCAGCAAGATATATTGCAGATAGATTTTTGCCTGATAAAGCAATAGATTTAATTGATGAAGCAGCATCAAATATAAAAACTGAAATGAACTATTTGCCTGAACCTCTAGAAAAAATTAATTATCAAATTGCTAGATTAGAAATTGAAAAAGCTGCTCTAACAAATGATAGTGAAACAAAGCAAACAGCCGTTAAAAATAAAGAAAGAATTTTAGAAATAGATAAAGAACTAAATACTTTAAAATTGCAAAAAGTTGACCTTGAAAAACGTTGGAAGCAAGAAAAAGAAGATATTCAAAAATTTTCTTCAATTAAAGAAAACATTGAAGAATTGAATAGAAAATTGCCTTTACTACAAAGTGAAGGAAAATATGTCGAAGCTTCTAAAATTATGTATGTTTTAGTTCCTGAATTAATTAAAACTAGAGATGAATTGGAACAAAAAATACAATCAAGGAAAAATCGTTTAATCAAAGAAGTTGTTGATGCTGAAGAAGTTGCTGACATTGTTTCAAAATGA", "aa": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAVKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSK", "aa_hexdigest": "357420ed61bcc4761cb8d0dfcaa24ef5", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001C99829F", "query_cov": 0.8933002481389578, "subject_cov": 0.975609756097561, "identity": 0.531, "score": 371.0, "evalue": 1.09e-123, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0AA38HIJ9" }, "genes": [ "clpA", "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 37195, "stop": 38709, "observations": { "mutation": [ 37500 ], "stop": [ 37500 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.5628491620111732, "identity": 0.998, "score": 753.0, "evalue": 4.9e-269, "gene_start": 1, "gene_end": 403, "reference_start": 1, "reference_end": 403, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.996031746031746, "score": 964.0, "evalue": 0.0, "description": "Internal stop codon at 37500. Nonsense mutation around 37500.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "LMJBGEANGM_111", "locus": "GCA013368015_00071" }, { "type": "cds", "sequence": "contig_1", "start": 39387, "stop": 39620, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATTTATTGCCATGGGTTTCAGAAATAAATTCATCACTGTTTTTTATTATACTTTCAACCATTAATACAACTGTATTAGGTGCTTTATCAACTTTTTGTCTTTTTATTGCTACAAAAATTAGTATTGAAATAATAAAAATTAAAGCAACTAAAATAATTAAAGTAAATAATTGGTTGTCATTGAAACTGCTTACTTCGCCAGGATTAACTGGACCACCAAATCAATGA", "aa": "MSNLLPWVSEINSSLFFIILSTINTTVLGALSTFCLFIATKISIEIIKIKATKIIKVNNWLSLKLLTSPGLTGPPNQ", "aa_hexdigest": "3a7ae195b9c7dd9ef93ba2e382f97518", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8432.02649999999, "isoelectric_point": 9.748022651672361 }, "id": "LMJBGEANGM_112", "locus": "GCA013368015_00072" }, { "type": "cds", "sequence": "contig_1", "start": 39630, "stop": 40037, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCAAAAATATCAAAATATAAATTAAATGCTGGCAAGATCAATAACCCAATAGGGTTTAACTCAGCTGAAGGTGATTCTAAGTTAGCGCCAATAATTTTGGTTCAAATATTATTTAAGAATAAACTTACTAGTGCAACCAATACAACTCCTCCAAGTATGTTTCCATACATACGGAAAGTTAAACTTATTAATGGTGAAAAGTTTGATGATGCTTCTAAGGGATTAAGAATTTTAAATAAATACTTAAATTTTTTATATATGAATCCAATGATGTGTGTTCCAAGTCAACTTATAACAGTGGCAGCAAAAATTGCTGACAAACTAGTTCCAATTGGGGCTAAACCAACTAATGAAAGCATATTTCCTAAAATCAAGAAGGCAAATAATGAAATAAAATAA", "aa": "MKLSKISKYKLNAGKINNPIGFNSAEGDSKLAPIILVQILFKNKLTSATNTTPPSMFPYIRKVKLINGEKFDDASKGLRILNKYLNFLYMNPMMCVPSQLITVAAKIADKLVPIGAKPTNESIFPKIKKANNEIK", "aa_hexdigest": "cd316ae9f96adac2d24ff4871f142a36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14983.714699999984, "isoelectric_point": 10.005122566223147 }, "id": "LMJBGEANGM_113", "locus": "GCA013368015_00073" }, { "type": "cds", "sequence": "contig_1", "start": 40235, "stop": 40540, "strand": "+", "frame": 2, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_036433167.1", "SO:0001217", "UniParc:UPI000512B8A3", "UniRef:UniRef100_UPI000512B8A3", "UniRef:UniRef50_B3PLV3", "UniRef:UniRef90_D1J7G1" ], "nt": "ATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTAAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTTTAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAA", "aa": "MDKLENIVNAFNTASAGVNSKEAGVNYPLAYGLTMLGAGIAVLGSGLVSIGQGLAVAKACEAVGRNPEAFSKIRTLLILGLGIVETGSIYCLVISLLLIFV", "aa_hexdigest": "21d84cdd637c46775f7a25fafd278151", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512B8A3", "ncbi_nrp_id": "WP_036433167.1", "uniref100_id": "UniRef100_UPI000512B8A3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512B8A3", "RefSeq:WP_036433167.1", "UniRef:UniRef100_UPI000512B8A3" ] }, "ips": { "uniref100_id": "UniRef100_UPI000512B8A3", "uniref90_id": "UniRef90_D1J7G1" }, "psc": { "uniref90_id": "UniRef90_D1J7G1", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_B3PLV3", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV3" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "LMJBGEANGM_114", "locus": "GCA013368015_00074" }, { "type": "cds", "sequence": "contig_1", "start": 40545, "stop": 40670, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTTAATTGA", "aa": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFN", "aa_hexdigest": "14512e228ffe99de195f5f6ed3e87fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4401.906899999999, "isoelectric_point": 8.254934883117677 }, "id": "LMJBGEANGM_115", "locus": "GCA013368015_00075" }, { "type": "cds", "sequence": "contig_1", "start": 40713, "stop": 41126, "strand": "+", "frame": 3, "gene": "atpF", "product": "ATP synthase subunit b", "db_xrefs": [ "COG:C", "COG:COG0711", "SO:0001217", "UniRef:UniRef50_B3PLV4", "UniRef:UniRef90_D1J7G2" ], "nt": "TTGATTATTACTTTATTGGTTTATAAACCCATAAAGAAATTAATTAAAAACCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAGGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCAGAAAGTGAAAGAATTGTCAATGCAAGCACGATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCGCAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAA", "aa": "MIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED", "aa_hexdigest": "be3df468688ee76264db8a0e355bc9c6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "atpF" ], "truncated": "5-prime", "pseudogene": { "start": 40545, "stop": 41126, "observations": { "mutation": [ 40668 ], "stop": [ 40668 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G2", "query_cov": 1.0, "subject_cov": 0.7098445595854922, "identity": 0.993, "score": 228.0, "evalue": 2.8e-74, "gene_start": 1, "gene_end": 137, "reference_start": 57, "reference_end": 193, "reference_sequence": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFNWPFFVFSLISLGLVVLIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED" }, "paralog": false, "identity": 0.9948186528497409, "score": 344.0, "evalue": 1.87e-122, "description": "Internal stop codon at 40668. Nonsense mutation around 40668.", "psc": { "uniref90_id": "UniRef90_D1J7G2", "gene": "AtpF", "product": "ATP synthase subunit b", "uniref50_id": "UniRef50_B3PLV4", "cog_id": "COG0711", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G2", "UniRef:UniRef50_B3PLV4", "COG:COG0711", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV4" ], "product": "ATP synthase subunit b" } }, "id": "LMJBGEANGM_116", "locus": "GCA013368015_00076" }, { "type": "cds", "sequence": "contig_1", "start": 41200, "stop": 41667, "strand": "+", "frame": 1, "gene": "atpH", "product": "F0F1 ATP synthase subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_D1J7G3", "UniRef:UniRef90_D1J7G3" ], "nt": "TTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTATTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCATTTTCAAAATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAGGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAA", "aa": "MALVSNEAAEITRLLKKNKDYLYVLNSYDIDENEKYRLIDEAFSKYNFYLVNTLKLAAKQHVIKYIIVILTKFVELSNEKRNIKYGFVYTISPLSERELKRLEVKLSNELKANIHLTNEIDPKLIAGIKIKVDDYLIDNSIVNKLNDFKKVVENN", "aa_hexdigest": "18d7ccde6f5b49b1e88707f70275fc6a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7G3", "query_cov": 1.0, "subject_cov": 0.8659217877094972, "identity": 0.9940000000000001, "score": 295.0, "evalue": 1.6e-100, "valid": true, "gene": "AtpH", "product": "F0F1 ATP synthase subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G3", "cog_id": "COG0712", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_D1J7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G3" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "LMJBGEANGM_117", "locus": "GCA013368015_00077" }, { "type": "cds", "sequence": "contig_1", "start": 41680, "stop": 42426, "strand": "+", "frame": 1, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATAGGCCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCCGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGTAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTAACCATTTATCTGGCCGTGTGTTAGATGCGCTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAGATTGCACCTGGTGTTATGACTAGAAAAGAAGTTAATCAACCACTTGAAACCGGGATTTTAGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGTAAGACCGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAACGTTTATTGTATTTATGTTGCTGTAGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGA", "aa": "MSLEPNDLSAIIKAQIKHFNEDISKQEIGRVITVGDGIALVSGLSNVEYGELVKFESGVIGMALNLEEDLVGIVVMGDDRNIVENSTVYRTKQVISTPVGNHLSGRVLDALANPIDGKGPIQYEYTRPIFKIAPGVMTRKEVNQPLETGILAIDAMIPIGKGQRELIIGDRQTGKTAIAIDAILNQKGRNVYCIYVAVGQKNSTIAQIVDQLAKHDALKYTTIISASASDSAPLQYIAPYTGITIAEE", "aa_hexdigest": "9c6688dc667927208fb85f72a3d4e291", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9717741935483871, "subject_cov": 0.9450980392156862, "identity": 0.598, "score": 292.0, "evalue": 6.86e-97, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "LMJBGEANGM_118", "locus": "GCA013368015_00078" }, { "type": "cds", "sequence": "contig_1", "start": 42427, "stop": 43128, "strand": "+", "frame": 1, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "nt": "ATGGCAAGAGGTAAGGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCTGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCAATAACTGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGA", "aa": "MARGKDVLIIYDDLSKHAVAYRTLSLLLRRPPGREAYPGDVFYIHSQLLERAARVNSDYGGGSITALPIIETQAEDIAAYIPTNVISITDGQIFTKEALFNSGQRPAIDIGYSVSRVGSAAQTKLTKKVVSSLKLELAQYNEMLAFAQFGSDLDQATRTVLDHGAKVYELLKQPQYSPYSSIEQIIILFCTKYRLINVIPKEQILKYKTDILNYFRTNPNAINTLRAVSEAND", "aa_hexdigest": "b468ffcffd554434878d00167a9a7f5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9F0", "query_cov": 0.9141630901287554, "subject_cov": 0.841897233201581, "identity": 0.653, "score": 274.0, "evalue": 7.09e-90, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9F0" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "LMJBGEANGM_119", "locus": "GCA013368015_00079" }, { "type": "cds", "sequence": "contig_1", "start": 43242, "stop": 43460, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00231.24" ], "nt": "ATGGAAAGTTTGCAAAAAATAAAACATAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTAGCAACTGCTAAGTTTGCTAAGATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACTTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGCGCTTCTTGA", "aa": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSAS", "aa_hexdigest": "04b8f1f836b6c54b2edfbab0ccaf8896", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00231.24", "name": "ATP-synt", "length": 64, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.90193751063153e-18, "score": 62.676998138427734, "start": 3, "stop": 66 } ], "seq_stats": { "molecular_weight": 8315.497499999998, "isoelectric_point": 9.85201015472412 }, "id": "LMJBGEANGM_120", "locus": "GCA013368015_00080" }, { "type": "cds", "sequence": "contig_1", "start": 43536, "stop": 44123, "strand": "+", "frame": 3, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "SO:0001217", "UniRef:UniRef50_D1J7G5", "UniRef:UniRef90_D1J7G5" ], "nt": "ATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGCAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCTGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGCCAAAGAAGAACGGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATACAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAA", "aa": "MYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN", "aa_hexdigest": "6ed4b614693c566f03fdcc8ec32644f5", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 43242, "stop": 44123, "observations": { "mutation": [ 43458 ], "stop": [ 43458 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G5", "query_cov": 1.005128205128205, "subject_cov": 0.6666666666666666, "identity": 0.995, "score": 362.0, "evalue": 6.82e-125, "gene_start": 1, "gene_end": 195, "reference_start": 99, "reference_end": 294, "reference_sequence": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSASWKFKSKRNLYIVFGSDLGLCGPYNSEMYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSTNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN" }, "paralog": false, "identity": 0.9931972789115646, "score": 561.0, "evalue": 1.4e-205, "description": "Internal stop codon at 43458. Nonsense mutation around 43458.", "psc": { "uniref90_id": "UniRef90_D1J7G5", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G5", "cog_id": "COG0224", "cog_category": "C", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G5", "EC:7.1.2.2", "UniRef:UniRef50_D1J7G5", "COG:COG0224", "COG:C", "KEGG:K02115", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G5" ], "product": "ATP synthase gamma chain" } }, "id": "LMJBGEANGM_121", "locus": "GCA013368015_00081" }, { "type": "cds", "sequence": "contig_1", "start": 44140, "stop": 45627, "strand": "+", "frame": 1, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_020002442.1", "SO:0001217", "UniParc:UPI00036A1184", "UniRef:UniRef100_A0A2K9YSL9", "UniRef:UniRef50_Q5FKY0", "UniRef:UniRef90_D1J7G6" ], "nt": "ATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGTGTTATCAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCATTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGTGTATTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATCGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTCGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGGGAAGGTAATGACCTTTATCACGAAATGCAAGCAAGTGGCGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTCAGATTTACACAAGCAGGTTCTGAAGTTTCTGCATTGTTAGGTCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACCTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTAGATCCAAATATAATTGGCCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGATGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGACGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAA", "aa": "MANKKTNENQEIQEEQNPVDESKNSENYGVISQILGSVIDVRFEEGHTPKILNALKIIDEDKKFSNEQILEVAQHVGNDTVRTIAMNLTIGLKRGMKVLDLGSPIKAPVGKEVLSRMFNVLGEPIDQKGGVFKHKMPIHRAAPSYEEQKSTLEIFETGIKVIDLLVPYIKGGKIGLFGGAGVGKTVLIQELINNIAKQHGGLSVFAGVGERTREGNDLYHEMQASGVLEKTALVFGQMNEPPGARMRAPFTALTIAEYFRDVMHQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAVEMGQLQERITSTNNGSITSVQAVYVPADDLTDPAPATTFTHLDAKTVLDRDIAALGIYPAIDPLASSSRMVDPNIIGLEHYNTARAVQNILQRFKELQDIIAILGMHELSEEDKKVVARARRIRNFLSQPFFVAEKFSGIKGQYIKLSDTIRSFKEILSGKYDELPEEAFLYVGTIDDVIEKAKRLGVNVEK", "aa_hexdigest": "4a19b542e91cd0c2c735d0c3c4fad8a1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036A1184", "ncbi_nrp_id": "WP_020002442.1", "uniref100_id": "UniRef100_A0A2K9YSL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036A1184", "RefSeq:WP_020002442.1", "UniRef:UniRef100_A0A2K9YSL9" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YSL9", "uniref90_id": "UniRef90_D1J7G6" }, "psc": { "uniref90_id": "UniRef90_D1J7G6", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q5FKY0", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q5FKY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5FKY0" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "LMJBGEANGM_122", "locus": "GCA013368015_00082" }, { "type": "cds", "sequence": "contig_1", "start": 45627, "stop": 46004, "strand": "+", "frame": 3, "gene": "atpC", "product": "ATP synthase epsilon chain", "db_xrefs": [ "COG:C", "COG:COG0355", "RefSeq:WP_175936636.1", "SO:0001217", "UniParc:UPI00158C3529", "UniRef:UniRef100_UPI00158C3529", "UniRef:UniRef50_A0A0C6FPC1", "UniRef:UniRef90_D1J7G7" ], "nt": "ATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGGCAGGCGCAACTGGGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCACCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCTGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAA", "aa": "METPKIHIIISTPLGIYFDKEASICTFTTTEGQIGIMAGATGFMAALVPSKIMVNFLNSPDSKVFYIDKGIVQFKNNVLSIIVNQIDTKEIELDHKFSPEDQKKYTIIEEVTLKKSILKSQTNNK", "aa_hexdigest": "21bed5e20c0ab9bea1da572ac01008a5", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158C3529", "ncbi_nrp_id": "WP_175936636.1", "uniref100_id": "UniRef100_UPI00158C3529", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C3529", "RefSeq:WP_175936636.1", "UniRef:UniRef100_UPI00158C3529" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C3529", "uniref90_id": "UniRef90_D1J7G7" }, "psc": { "uniref90_id": "UniRef90_D1J7G7", "gene": "AtpC", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A0C6FPC1", "cog_id": "COG0355", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A0C6FPC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C6FPC1" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "LMJBGEANGM_123", "locus": "GCA013368015_00083" }, { "type": "cds", "sequence": "contig_1", "start": 46260, "stop": 46892, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215D6119" ], "nt": "ATGAAAAAAATAACAAAAGCTTTATTAATAATATGCACAACTATAACACCTAGTTTTGCTATACCGTTAATATCGGCAGGATGTAATAAAACAACAAATCATGAAACAAACAAAGAAAACACAATTATAAATAGACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAACCCCAATAAGTTAGAATCAAATAGTGAATTAGCAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGATTTCAATGTTTGACAAATTAAACAAAGAAAACTTGTCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTCAATGATGGAGATAAAATCGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAATAACGAAAGCTATTTAAATGGATTATTGAATGATAATGACACAATGTTTAATTTAACAACTAATCTCAAAAAAGGTAATGAAATAGATGCATATTATTTAGTTGATGGTCAAGGTTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGA", "aa": "MKKITKALLIICTTITPSFAIPLISAGCNKTTNHETNKENTIINRQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKKFKIEILKLEADKKNNLISISYNISFNDGDKIAKLENQKYSLTGLSTNNESYLNGLLNDNDTMFNLTTNLKKGNEIDAYYLVDGQGSISLLASNKNVYVPKDLILEYT", "aa_hexdigest": "1a3711503595004866d529ab127b8057", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 46260, "stop": 47195, "observations": { "mutation": [ 46890 ], "stop": [ 46890 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D6119", "query_cov": 1.0, "subject_cov": 0.40540540540540543, "identity": 0.938, "score": 370.0, "evalue": 2.52e-124, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MKKTAKALLIICTTITPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKNFKIEILKLEADKKNNLISISYNISFNDGNKITKLENQKYSMTGLSTNNESYLNGLLNDNDTMFNLTTNLKKGSEIDAYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLNFVEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKN" }, "paralog": false, "identity": 0.9292604501607717, "score": 573.0, "evalue": 1.1e-206, "description": "Internal stop codon at 46890. Nonsense mutation around 46890.", "psc": { "uniref90_id": "UniRef90_UPI00215D6119", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D6119", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23649.578600000015, "isoelectric_point": 8.31605091094971 }, "id": "LMJBGEANGM_124", "locus": "GCA013368015_00084" }, { "type": "cds", "sequence": "contig_1", "start": 47373, "stop": 48323, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215CD1C0" ], "nt": "TTGACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAGTCAAAGTCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAATTAGAAAATCCTTTAGATTTTGCAGAATATTTTGCCAAAAATGGAATTAACAATTTTGATAAAAATTATATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAATGAAGTAGGTAAGGAATTTTTAGGAAATCCAAATGAAATTTTGGCAAGTGATTTCTCAGCTACAAATTTAAAATTGAATAAAAATGTTGGCGATAGTCTTGATAGTTTAAATTTTATTAAATTATCTTCAATAAAAAGACCTTCAAAAAATTCAGAGGAATTAAAGCTCAACGAAGAAGGTAAGGCAACGTTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAAGTAGAAAAAGCTGATTATAAAAAACAACACACCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGTGGTTGGGCAACTGCCGAAGAAATAAACGAATTGGAAACTTTTTTGCCAAGCCAAAAAAAATCTAAAAAAAATAAGAGAATAAACATTGAACATTATATAAACAATGGCAATATAGAAATACTTGTTGGAAGAGTAATTACTAAAAAAAATAAAATTACTTTCGGCGATGCATTTAAAAGACAAAAAGAATTTAAAGAAATAGTATTTGATGAAGAATTTGTTGAAGCAGTTAAGAAATCATCTAATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGATGAAAATTCTAAACATAAAAATCAAAAGAATTGA", "aa": "MTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLDFAEYFAKNGINNFDKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYTKEVVFENLKKVEKADYKKQHTSLIQKINDKKIIKSGWATAEEINELETFLPSQKKSKKNKRINIEHYINNGNIEILVGRVITKKNKITFGDAFKRQKEFKEIVFDEEFVEAVKKSSNNKSENKILSNVITDENSKHKNQKN", "aa_hexdigest": "a8461ff6c42fbb649a5dd5dc9652eba0", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 47073, "stop": 48419, "observations": { "mutation": [ 48321, 48351 ], "stop": [ 48321, 48351 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215CD1C0", "query_cov": 1.0, "subject_cov": 0.5851851851851851, "identity": 0.968, "score": 578.0, "evalue": 3.15e-204, "gene_start": 1, "gene_end": 316, "reference_start": 193, "reference_end": 508, "reference_sequence": "AYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLDFAEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYIKEVVFENLKKVEKADYKKQHASLIQKINDKKIIKSGWATAKEIKKLETFLPSQKKSKKNKGINIEHYINNGNTEILVGRVITKKNKITFGDAFKRQKEFKEIVFDEEFVEAVKKSSNNKSENKILSNVITDENSKHKNQKNWIRLTKHEGKWCILFTFIEDTKSTPYYVQIEK" }, "paralog": false, "identity": 0.9486607142857143, "score": 835.0, "evalue": 4.16e-308, "description": "Internal stop codon at 48321, 48351. Nonsense mutation around 48321, 48351.", "psc": { "uniref90_id": "UniRef90_UPI00215CD1C0", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215CD1C0", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36729.213200000035, "isoelectric_point": 9.105014991760253 }, "id": "LMJBGEANGM_125", "locus": "GCA013368015_00085" }, { "type": "cds", "sequence": "contig_1", "start": 48659, "stop": 49321, "strand": "+", "frame": 2, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF", "UniRef:UniRef90_UPI00215D5ADF" ], "nt": "ATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGGACAATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCTAAATAAACCAGAAGGTGAAAAACCTGGAACAACTCCTGAAAAGAAGCCTGAAGTTAAGCCGGCGGGTGAAAACCCCGGAACAACTCCCGAAGTTAAGCCAGGATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGGTTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAACAACTTCGATAAGAAATTCAAAGTAGAAGTTTTAAAATTAGAAGTAGATAAGAAAATTAATTCAATATTAATTACATACAGCATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAACAATGAAAGCTATTTAAGTGGATTATTGAACGATAAGGATACAGTATTCAATTTAGGTGATGCTTCTAATGAAGATAGTTTAACAACAGTTGAACAGTTAGATGCATATCATTTAGCTAATGGCCAAGGTTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCTAAAGATCTAATCTTAGAATATACTTGA", "aa": "MKKSKKILLTMGTILPLVISAPLVAAGCKELNKPEGEKPGTTPEKKPEVKPAGENPGTTPEVKPGSNSDLNDLTEAVKKANFQYKDIDKVSMFDKLNKENLSNNFDKKFKVEVLKLEVDKKINSILITYSISFNDGSKDAKLENQKYSLTGLSTNNESYLSGLLNDKDTVFNLGDASNEDSLTTVEQLDAYHLANGQGSISLLASNKNVYVPKDLILEYT", "aa_hexdigest": "74bba9c7226a1b369e4a8f6068400ede", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 48659, "stop": 49624, "observations": { "mutation": [ 49319 ], "stop": [ 49319 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D5ADF", "query_cov": 1.0545454545454545, "subject_cov": 0.5686274509803921, "identity": 0.8490000000000001, "score": 319.0, "evalue": 1.17e-105, "gene_start": 1, "gene_end": 220, "reference_start": 1, "reference_end": 232, "reference_sequence": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPEDPKKPEGEKPGTTPEVKPKSNSDLNDLTEAVKKANFQYKDIDKISMFDKLNKENLSNNFDKKFKVEVLNLEVDKKINSVLITYNISFNDGSKDAKLENQKYSLTGLSTNNESYLNGLLNDKDTVFNLGDASNDDSLTTVEQLDAYYLVDGLGSISLLASNKNVYVPEGLMLEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSINSFKEGDIEQTLKNNFIAKFEKSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVSGFKGVEKVDDEQVKKIVDKLTFGDVYFPEKIKDF" }, "paralog": false, "identity": 0.8738738738738738, "score": 520.0, "evalue": 2.16e-187, "description": "Internal stop codon at 49319. Nonsense mutation around 49319.", "psc": { "uniref90_id": "UniRef90_UPI00215D5ADF", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D5ADF", "UniRef:UniRef50_UPI00215D5ADF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF" ], "product": "variable surface lipoprotein" } }, "id": "LMJBGEANGM_126", "locus": "GCA013368015_00086" }, { "type": "cds", "sequence": "contig_1", "start": 50021, "stop": 50455, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCCGCAACATTAAAATTAAACGGCAAGGAATATAATGTTGAAACAACATTAACTCATGAATTAAAATTAGATGAATGGGTAGATGAAGTCGGAAAAGAAATTGATGGGGATTCAAATACCATTCCCGTAAATGAATTTTCTAATAAAAATTTAAAATTGAATAAAAAAATTAATGACTTCGTCAATGGTTTAGACTTTGTTCAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAGGCAGAAAAAAAATTGAATTTAGAAGGTAAGGCAACCTTAATTTATGAATTTAAAAATATATATGATAATTCAACATATAGAAAAGAAGTCGTATTCGAAAATCTAAAAAAAGCAGAAAAAGTTGATTATAAAAAACAATATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGA", "aa": "MSATLKLNGKEYNVETTLTHELKLDEWVDEVGKEIDGDSNTIPVNEFSNKNLKLNKKINDFVNGLDFVQLNSIKRAKSSEAEKKLNLEGKATLIYEFKNIYDNSTYRKEVVFENLKKAEKVDYKKQYASLIQKINDKKIITNNK", "aa_hexdigest": "0792fe4b7b4a9cbc4e3bc91e3af5b33c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16665.739799999996, "isoelectric_point": 8.857198524475098 }, "id": "LMJBGEANGM_127", "locus": "GCA013368015_00087" }, { "type": "cds", "sequence": "contig_1", "start": 50597, "stop": 50725, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATTTTGGAGATAATAGCAAAACTAAAGAATCATTCAAAGATTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAGGATTCCGGACATGGGGAATGA", "aa": "MHFGDNSKTKESFKDLKFDNDFLEAIKSKTLVNVVKDSGHGE", "aa_hexdigest": "e402c1ec5da5c4d9933bb22161ae6b0a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4757.250200000001, "isoelectric_point": 6.043485450744629 }, "id": "LMJBGEANGM_128", "locus": "GCA013368015_00088" }, { "type": "cds", "sequence": "contig_1", "start": 50933, "stop": 51871, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1", "UniRef:UniRef50_UPI00215D9390", "UniRef:UniRef90_D1J7H1" ], "nt": "ATGGTTAAGAAAAAAGTATTATTTAAAATATTTGCTATGGGTACTATTCTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAATGATACAGAAAATAAAGATAATAGTTTTGAAAATAACACTAATAACGGTCAAAGCAACCAACATGATTTAGGCACCGAAGATAATAATTCAAGAAATTTGATAGTTCCTGAATCAAATAATAAGCCCAAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTACCCCAACGCCCGAACCAGCGCCTGAGTCAACACCTGAAATAAAGCCTGAGCCAACTCCCAAACCAGCACCCGAACCAAAAGAAAATGATCATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTACTAGAAAAAAAGGAGAATACATTGAACCAAAGCCGTCGATTTTATTGGCTGATAGGTCAAATAAACAGTCGGATTTGAATAAACAAGGAGCCCAAGAAGTAAGCGAATTCCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCTAATTTTGTTATTTACGGTGGCGATACTAATATAAAAAATGAAAATTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAAGCTTAAAATTAAAAAAATATGATCAAAAATTTCTAACATCATTAGGAACAAGAGGAAAATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTCAAACCAAAAATAATTACCAATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAGCTTGAAAAATTAAAAGGATGA", "aa": "MVKKKVLFKIFAMGTILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQSNQHDLGTEDNNSRNLIVPESNNKPKNDSIPQPESKPNITPTPEPAPESTPEIKPEPTPKPAPEPKENDHKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADRSNKQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRSLKLKKYDQKFLTSLGTRGKYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELEKLKG", "aa_hexdigest": "67472329cd6aa2a1560fa1f71db58663", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D9390", "query_cov": 0.8814102564102564, "subject_cov": 1.0073260073260073, "identity": 0.898, "score": 423.0, "evalue": 2.97e-147, "valid": false, "uniref50_id": "UniRef50_UPI00215D9390" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9390" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 50933, "stop": 52171, "observations": { "mutation": [ 51869, 52040 ], "stop": [ 51869, 52040 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H1", "query_cov": 1.0544871794871795, "subject_cov": 0.6553784860557769, "identity": 0.86, "score": 468.0, "evalue": 2.82e-161, "gene_start": 1, "gene_end": 312, "reference_start": 1, "reference_end": 329, "reference_sequence": "MTKKKVLFKIFAMGAILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQRNQHDLSTEDNNSRDLIVPESNNKPENDSIPQPESKPNIIPTPEITPKPAPEITPEPTPKPAPEITPEPTPKPAPEITPKPNENDYNKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADSSNRQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRDLKLKKYDQKFLTSLGTSGKYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELENLKGWKSKNNDKSTIRSQISDHAPVFTDVVFSNPIETLKVNESLRKNQTLSKEQNTIRIAHWNILNFGGPNQDAKTMAIATTIYKSGFDIIGLTEINDGRGNYVQNIVDLLNEYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN" }, "paralog": false, "identity": 0.8438228438228438, "score": 639.0, "evalue": 2.29e-231, "description": "Internal stop codon at 51869, 52040. Nonsense mutation around 51869, 52040.", "psc": { "uniref90_id": "UniRef90_D1J7H1", "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H1", "UniRef:UniRef50_D1J7H1" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35396.53289999999, "isoelectric_point": 8.932691001892092 }, "id": "LMJBGEANGM_129", "locus": "GCA013368015_00089" }, { "type": "cds", "sequence": "contig_1", "start": 52085, "stop": 52390, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017", "UniRef:UniRef90_UPI00215DB2CF" ], "nt": "ATGGCTATTGCTACTACAATTTATAAGTCTGGTTTTGATATAATAGGCTTAACTGAAATTAATGATGGAAGAGGAAACTATGTGCCAAATATAGTTAACCTTTTAAATAAATATGCAGGAAAAGAAAGATATAAATTTGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCGCAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCGAAGCTATTTAAAACCAATTAAATACTACAAAGATAACTAA", "aa": "MAIATTIYKSGFDIIGLTEINDGRGNYVPNIVNLLNKYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN", "aa_hexdigest": "b61dbab2e10f931e55243f7c7665ba89", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00215DB2CF", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.95, "score": 190.0, "evalue": 3.9e-61, "valid": true, "uniref50_id": "UniRef50_A0A449B017" }, "pscc": { "uniref50_id": "UniRef50_A0A449B017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11727.157699999992, "isoelectric_point": 9.397121238708493 }, "id": "LMJBGEANGM_130", "locus": "GCA013368015_00090" }, { "type": "cds", "sequence": "contig_1", "start": 52983, "stop": 53408, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCAATTGAAAAAATTAAATCGGCATTTACCTTCAATGATGTTTTAGTATTATCTGAATTAGTAGGCGAAGATAAACCAATCAATGAAACTGCTTTGGTTAAATTAATCAATTTAGTATTTGGAAAAAGTAATTTTAATGACTCTGTAATTTATAATGCATTAAGAAATTTAAACAACAATTCAGATGAATCAAAACGTTCAAACATGAATACCTTAAATAGTTCTGTTTCAAATGCGTTAGGAAGCATTTTCAAATCTGCAAAAGCCACTGATAAAGATGACCCTGATAATATTGAATATTCCGCAGATGTTTTAAAATTGCTTGATACAATTTATAAATTGCTTGCAAAGGCATACAATAATGATAAGGATGCAACTTCACCAACATATAAAGTTAGGGCACAATCTGAAGCATGA", "aa": "MNDAIEKIKSAFTFNDVLVLSELVGEDKPINETALVKLINLVFGKSNFNDSVIYNALRNLNNNSDESKRSNMNTLNSSVSNALGSIFKSAKATDKDDPDNIEYSADVLKLLDTIYKLLAKAYNNDKDATSPTYKVRAQSEA", "aa_hexdigest": "79b48327741e17e9f8e07431da57baf6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15572.22459999998, "isoelectric_point": 4.977924919128417 }, "id": "LMJBGEANGM_131", "locus": "GCA013368015_00091" }, { "type": "cds", "sequence": "contig_1", "start": 53853, "stop": 54542, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGCAAATAAAGATAAATTTAAAGCATTTATTAAAAATGCAGTAAATGAAATAACTTCTAACAAAAACTTAATTAACAAATCTATTGAATATGCATTGAATTTATTCAACAAGAAATATTCAATTAGTTCAAGTAGCGCTCAAATTCAAGGAATTGCAAATCTTTTGACTAGATTAATTAACAAACTAGGTTCAATGAATGTATTTAGCAATATATTGGGAGATTTCATTGATTTATTTGTTGATGTTGATTTCCTTGACAATAATGGTCATTTCAATGGAAATGCTTTGGCATCCAAACTTCTTGAATCTATTAAAAAGATTGAATACCATAAGTATTTAACAACAGAAAATTTAAGTGAATTATTTGAAACAATATTAGACAAAAATATTGATAAAAATGTCTTGTATAATGAATTGAATTTCGCTTATTCATATATAGTCGACAATCTTGATAAATTTAAACAAGCATTTTCTGCTAAAAGCAATTCTACAACATTCAACGAAGTAGATAAAACTAAACAAGATAAGGAACACGAACAATTCCTAAAGAATCTAGAAAAATTAATTTACAATGCCTTAAAAGCTCTAAATGGATCAATAAAAGCAGATAATAAAAATGGAAAAGAAGCATTAGTTGATTTTGTATATAATGTTTTGAAGGATCAAATTAAAAAATTTAATTGA", "aa": "MSANKDKFKAFIKNAVNEITSNKNLINKSIEYALNLFNKKYSISSSSAQIQGIANLLTRLINKLGSMNVFSNILGDFIDLFVDVDFLDNNGHFNGNALASKLLESIKKIEYHKYLTTENLSELFETILDKNIDKNVLYNELNFAYSYIVDNLDKFKQAFSAKSNSTTFNEVDKTKQDKEHEQFLKNLEKLIYNALKALNGSIKADNKNGKEALVDFVYNVLKDQIKKFN", "aa_hexdigest": "0075d354bca6d8b477b611fa27bc515b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26152.42050000003, "isoelectric_point": 8.738189888000488 }, "id": "LMJBGEANGM_132", "locus": "GCA013368015_00092" }, { "type": "cds", "sequence": "contig_1", "start": 54582, "stop": 54872, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAATGCGTATTTTCGATATTAGATAATAAAGCAAAGAAAGAAATTAAAGATGAATTAAAAAATTCAGTTGATATTTTCTTACAAAGTAATAAATTTAAACAATATATCAAACACAATTTATTAAAAATAAAAGAAAAAATTCTAAAAATTGATTCAAAAGCTTCTGAATTAGGCAATGAAATAGTTGAAACATTAATGAAGGTAATTTTTGATTCAAATAATACAAAAGAATTTATACTAACATTATTAAATTCATTGATAGATTTAGGCGATAACAATCAATGA", "aa": "MFKCVFSILDNKAKKEIKDELKNSVDIFLQSNKFKQYIKHNLLKIKEKILKIDSKASELGNEIVETLMKVIFDSNNTKEFILTLLNSLIDLGDNNQ", "aa_hexdigest": "0d9608e5535c427f7145cd25511e24eb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11133.903299999987, "isoelectric_point": 8.644839668273928 }, "id": "LMJBGEANGM_133", "locus": "GCA013368015_00093" }, { "type": "cds", "sequence": "contig_1", "start": 55134, "stop": 55319, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAATCTAAAGAATATTACATGGCCTGTTAATGCAAAAGAAACATTACTAAAATTAATTTCGCTAGGTATTGAAAAATTGCCAGAATTCAATATTCTAGATGGCTTAATTGAAAATGTTTTAGACTACTCAGTCAATAATCTAAACAAGAATCAATTTGAATTTAAAAAGTACGATTGA", "aa": "MEQNLKNITWPVNAKETLLKLISLGIEKLPEFNILDGLIENVLDYSVNNLNKNQFEFKKYD", "aa_hexdigest": "d40a13970fb1dace2edb7de9b0fff0c5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7154.153400000001, "isoelectric_point": 4.964397239685058 }, "id": "LMJBGEANGM_134", "locus": "GCA013368015_00094" }, { "type": "cds", "sequence": "contig_1", "start": 55452, "stop": 55646, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATACATTGATGAAATAAATATTTCAAGCTTTAAGGGTGATGTACAAGAAAACCTTCAACAAATAATTTCATATATAAAATCATTTGTTGAAACAAGTTACAATGAAGATCTTTTACCTTTAATAAAAGAGCTAATTAAAAAGTTATTTGATGATAATAATTTAAAAGATTTAAATAATATTTCTCAATGA", "aa": "MKYIDEINISSFKGDVQENLQQIISYIKSFVETSYNEDLLPLIKELIKKLFDDNNLKDLNNISQ", "aa_hexdigest": "ca8f3ab292e5527901cf62fcaba3ec50", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7524.463199999997, "isoelectric_point": 4.504399299621583 }, "id": "LMJBGEANGM_135", "locus": "GCA013368015_00095" }, { "type": "cds", "sequence": "contig_1", "start": 55869, "stop": 56162, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCCGAATTTATCAATAAAATATTGAAAAATCTTGCAGACAACAAGGGCTTTGATGCGTTAGAACAATTGCTAGAAAATGTTTTATCAAATAAAGATATAACCCAAAAATTAGTTGAATTTTTAGTTGCGTTTTTAGAAATTGAAGCAAAAACAAAATTGAATTCTGACGAAATTAACCAATTAACTAATTATTTCAACAAGATTTTACCTACTTCATTGAAATCTAATTTATATAAAAACTTAAAAATTAAATTAACAAATGAATTGAAGTCAATAAATAAATATTCTTGA", "aa": "MPEFINKILKNLADNKGFDALEQLLENVLSNKDITQKLVEFLVAFLEIEAKTKLNSDEINQLTNYFNKILPTSLKSNLYKNLKIKLTNELKSINKYS", "aa_hexdigest": "fee64ba08acf44f53f6561e0b279fe6c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11219.904699999983, "isoelectric_point": 8.803174018859863 }, "id": "LMJBGEANGM_136", "locus": "GCA013368015_00096" }, { "type": "cds", "sequence": "contig_1", "start": 56448, "stop": 56642, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGATACATTAAATGAAGAAAATGTAAAAACTCTATTTTTGATATTAATCAAGAATTTAAATGAATTTGCAAATGCTTTTTTAACAAACAACGACACAACAACACAAAAACAAAATGAAGGTTCAAAACCAATTAAATTAGACAATGATTTAAATTTTGAGCAAATTTTCTTACTAATTAAAAAATTATGA", "aa": "MIDTLNEENVKTLFLILIKNLNEFANAFLTNNDTTTQKQNEGSKPIKLDNDLNFEQIFLLIKKL", "aa_hexdigest": "a55973f7ac9b81d6411ffa57deb0a20f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7426.452799999998, "isoelectric_point": 4.853277015686035 }, "id": "LMJBGEANGM_137", "locus": "GCA013368015_00097" }, { "type": "cds", "sequence": "contig_1", "start": 57093, "stop": 57416, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACTATTTAAAATCAATATTTAGTGACTACTTCAACATTGAAAAAAATAAGGATTTAATTAATAATTTTGTTGATTTACTACTCGAAAAAGATAATAAATCAGAAGGAATAAAATTTTCTGAATTAATAAAAACACTTTCTGTTCTTGCTTCTAAATCTGAACTTGTTAACTACGTCTTTAAAAAATTTGACATTAAATCTATTATATTAAATTACTTGGATTCATTTAAAGACAACAAACTATCTAACGACACTGAATTAAATTCACTATTACAAACCTTAATCACCAAAACTAGAAATTACATAGATAGTCAATGA", "aa": "MKNYLKSIFSDYFNIEKNKDLINNFVDLLLEKDNKSEGIKFSELIKTLSVLASKSELVNYVFKKFDIKSIILNYLDSFKDNKLSNDTELNSLLQTLITKTRNYIDSQ", "aa_hexdigest": "ac41301de9990870ad66b58372c32438", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12527.222199999978, "isoelectric_point": 7.827445030212404 }, "id": "LMJBGEANGM_138", "locus": "GCA013368015_00098" }, { "type": "cds", "sequence": "contig_1", "start": 57828, "stop": 60062, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCACTAGATAACAGCAAAGAATTAGCTCATTTAAATCAAGTTATTTCCTATAATCAAAATTTACTTTCAAAATTTAATAGTACCAAATTGAATAATCTATTTGGTGATTTTTCTAATAAAAAATTACAAGAGCTAATTAAAAAAATAAAACAAGCAAACCTGATTACAATTTCTTTGGGATTCAACGACCTTTTTGAACAATCATCATTTTTTAAAGAAGCGTTTGATCTTTTAAGAGAAAACAAAAATAAAGAAGAAATTTTGAAATATTTTTCAAAAATTCTTCTTGAATTTAAAAGAAATTGTAAAAATTTTGAAGATTCATATTCAAAATTAATTTCAATCATTAAACAATATAATCCTGAGGCAAATATTAATCTTGTGGGTTATATATCACCTTATTTGAAATTAACAAAATTAATTGACAAACAATACAATATTTCATTGACTAATGATTGCATAGAAGAATTAAACAAATCATTAGCAAACATTGCCAAAACAAACAAGCTAAACTATTTAAACATTAATAATGACATTAAAAATCTCAAAGATAATGCCGATAAACTAAACAACAATTTCTTTGACTGCTTCCCATCTAATAATTTATACAAAAAATTAGCGCAAGATATTTTTATGAAAATGTCATTGCCTAATAATAAATATAAAGAATTAGTTGATAATAATTCTAATCTAAATGGATTTAGCCAATCACTTGAATTTAGCAAAACTCCTTCCGAAATTTTTTCAATGATTTTAGGAATAAATGGCAACAATGTAGATTCTTTTGAAAAAGAATATACATTCGAAGAAAATGCAGATATCAAAAATATTAATTCTTCATACAATTCAAAGCATATAATCCTTCAATTAAAATCAATGTTAAATTCAAACAAAAACATTGAAGATAAAGAAATTTTGAATACATTTTCAATACTATTTGGATTAATTGGCTTAGACCCAAAAGATTTAAACAGCTTTAATAAGTGTTTATTAGAATCTTTAAAGAATAAAGAAAGTAAAAAATCTATTATTAATTTTTTGAATCTTTTTATTGATTCAAAAAATTTAAGCAAATCGTTTTTAGATATTAATAACGCATTAAATAATGCTATTAATAAAAAATCATATGCAGGTTTAAACTTTGAAGAAATTAAAAAAATTGTTTATTCAACAACTACTTCTAAACAAAATATTTATGAAATTTTAAAAGAAATTTTTTATAACAAATCTTTAAGTCAAGATCTTAAAAGAAGCGAATATGCAAAATTATTTAAGGCCATATTTTCAGATGTATTCATTAAAAAAATATTAAAGAGATTATTGCCTCAAACTACTATTGATTTATTCAAAAATCTCTTCAATAACAAAGATATTGAAAAAGGTTTAGACACCATTTTCCAAAAAGTGTTGAATTTGTTAATTAATAATTCAGATAAATATTTTAAATTTGAAAAAATTGATGACTTTATTACTACATTGGTTCATGATTCAAGCAAAGATGTAAATGATTTAGTTAGCCTAATAATTAAAGAAGTTTCAAAAGATAGAAAGCTTGCAAACTACTTGACCGGGAAAATTGTTGATTCTTTATACAAATCATATGATTTAGATTTACTTGAAAAACCAAATATTTCTTTGTTTGTTTCAACATTTTTTGACAACATTTCAAACTTTGATGGTTTATCTCAAATATCAAACATTATTATTCAAATATTAATGAATTCTAAACTGGATGATGACCACCAAAATAGGGATTTAATTTCAGAAATTCTAAATGGAATTCTTTTTGATAAATACGACCTAAATAGGGATAACTCGCTTTTATTTAAAATCCTATCGTTTGAACCAAAAATACAAAATATTAACGACTACAAACAAGGATTAAAACACTTAGCCCTAAAATATGCAATTAAAAATAATGCATTAATGCCAAAAAACATAGGCAAATTAATTGATAAAGATTCAAGAGAAAACATATTAAGATTTTATAAAAATATAGCATTAAATGCAAAGAAGAATTTAAACGAATTTGGTAGTGAAATATTTAAAGATATTATCTCACTTCTAGTAAATGAATTATTTAAGAATAATAGTTTGTTCAGTCAATTGATTGATCAAATTGGAAATTATGTTGTTATTCAGCCTATCGTTGCTTTCATTAAATCAATTAATTACAAAAAGATATTCAACAATGAAGAAGAAATTCAAAAATTTGTTGAAAATGTTTGA", "aa": "MLSLDNSKELAHLNQVISYNQNLLSKFNSTKLNNLFGDFSNKKLQELIKKIKQANLITISLGFNDLFEQSSFFKEAFDLLRENKNKEEILKYFSKILLEFKRNCKNFEDSYSKLISIIKQYNPEANINLVGYISPYLKLTKLIDKQYNISLTNDCIEELNKSLANIAKTNKLNYLNINNDIKNLKDNADKLNNNFFDCFPSNNLYKKLAQDIFMKMSLPNNKYKELVDNNSNLNGFSQSLEFSKTPSEIFSMILGINGNNVDSFEKEYTFEENADIKNINSSYNSKHIILQLKSMLNSNKNIEDKEILNTFSILFGLIGLDPKDLNSFNKCLLESLKNKESKKSIINFLNLFIDSKNLSKSFLDINNALNNAINKKSYAGLNFEEIKKIVYSTTTSKQNIYEILKEIFYNKSLSQDLKRSEYAKLFKAIFSDVFIKKILKRLLPQTTIDLFKNLFNNKDIEKGLDTIFQKVLNLLINNSDKYFKFEKIDDFITTLVHDSSKDVNDLVSLIIKEVSKDRKLANYLTGKIVDSLYKSYDLDLLEKPNISLFVSTFFDNISNFDGLSQISNIIIQILMNSKLDDDHQNRDLISEILNGILFDKYDLNRDNSLLFKILSFEPKIQNINDYKQGLKHLALKYAIKNNALMPKNIGKLIDKDSRENILRFYKNIALNAKKNLNEFGSEIFKDIISLLVNELFKNNSLFSQLIDQIGNYVVIQPIVAFIKSINYKKIFNNEEEIQKFVENV", "aa_hexdigest": "680d58a778dcf0ea3fcfe2d3e451b2ff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 86274.10330000048, "isoelectric_point": 8.87151050567627 }, "id": "LMJBGEANGM_139", "locus": "GCA013368015_00099" }, { "type": "cds", "sequence": "contig_1", "start": 60069, "stop": 60437, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTACAAAAATCCTCAAGGCGATTGTTGCTTTAGGAATTACATCTTCAATTGCTTCTAGTTCATTAGTTGCTATATCATGCAAAGACAAAACTAAGAAGCCGGATACAAAAATAACTCAAAGTGAAAATAAAGTTTCTAAAAAAATTAATTATGTTGCATTAGGCGACGGCTTTGCTGCAGGTTATAATAGCAGCTCAAATTACTATTTCAACAATGAATATGATAGCAAAAACGATTTAGTTTTAGGAACATCGTATCCTTCATACATTGCTAATTTTATAAAACTATTATCTGATAAACAAACAACATTATCAACTTATTATAATTACGGTTTGCCACTTTCAAATGTTAATGATTGA", "aa": "MKKSTKILKAIVALGITSSIASSSLVAISCKDKTKKPDTKITQSENKVSKKINYVALGDGFAAGYNSSSNYYFNNEYDSKNDLVLGTSYPSYIANFIKLLSDKQTTLSTYYNYGLPLSNVND", "aa_hexdigest": "debf6fe0fcd68e5512917b80ea84e908", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13360.959999999986, "isoelectric_point": 9.320919609069826 }, "id": "LMJBGEANGM_140", "locus": "GCA013368015_00100" }, { "type": "cds", "sequence": "contig_1", "start": 60447, "stop": 61001, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3FC695", "UniRef:UniRef90_UPI001F3FC695" ], "nt": "ATGTCAACAAGATCAGCAGGTTTTTCAACTATAAATTATTATAATTTTACTCAACCTACAATTATGGTTCACGGTATCCTAATGTTTATTGGATTTTCTCCTGTTTCAACTGCTGGAGGAATTCGTAATATTACAGTGGCTATAATTTTCATGAGTATAATTACTTTAATTAAAAATCGCAAAAACATAAATGCCTTTCATAGACAAATAGGAAAAGAATCTTTAATTAAAGCAGTTAACATTTTTGCAATTGCAATTTTTATAACTCTAATAATGACAATAGTAATATATCATACACTTCCAAGCGATTTAAAAAATGTTTATAATGAGTCATATCCAATGGTATATGTAATGTTCGAAGTTTTCTCAGCATTCGGAAACTCAGGACTTAATATTGGATTAACACCAAATTTGGGTATTACTGGAAAAATACTTTTAATTATAACAATGATAGTAGGACAATTAGGAATATCACAATTTCTAAAAATTTTTACTTTTTCAAAATCAAAACCACAAAGATATCAATATATTTATGAAGATATTTCTATTGGTTAG", "aa": "MSTRSAGFSTINYYNFTQPTIMVHGILMFIGFSPVSTAGGIRNITVAIIFMSIITLIKNRKNINAFHRQIGKESLIKAVNIFAIAIFITLIMTIVIYHTLPSDLKNVYNESYPMVYVMFEVFSAFGNSGLNIGLTPNLGITGKILLIITMIVGQLGISQFLKIFTFSKSKPQRYQYIYEDISIG", "aa_hexdigest": "830c472d0e68ba1cfc99cb13039a07db", "start_type": "ATG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "3-prime", "pseudogene": { "start": 60447, "stop": 61208, "observations": { "mutation": [ 61061 ], "stop": [ 61061 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F3FC695", "query_cov": 1.0, "subject_cov": 0.7215686274509804, "identity": 0.995, "score": 345.0, "evalue": 5.63e-119, "gene_start": 1, "gene_end": 184, "reference_start": 72, "reference_end": 255, "reference_sequence": "MTKIKSIKKDNPRHCFTLFTKFTIITYIFTSLIGLLLTFIFETTAKGANYFWNSSEYGSIGDKVFAIYFQTMSTRSAGFSTVNYYNFTQPTIMVHGILMFIGFSPVSTAGGIRNITVAIIFMSIITLIKNRKNINAFHRQIGKESLIKAVNIFAIAIFITLIMTIVIYHTLPSDLKNVYNESYPMVYVMFEVFSAFGNSGLNIGLTPNLGITGKILLIITMIVGQLGISQFLKIFTFSKSKPQRYQYIYEDISIG" }, "paralog": false, "identity": 0.9920948616600791, "score": 493.0, "evalue": 9.58e-180, "description": "Internal stop codon at 61061. Nonsense mutation around 61061.", "psc": { "uniref90_id": "UniRef90_UPI001F3FC695", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI001F3FC695", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F3FC695", "UniRef:UniRef50_UPI001F3FC695" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F3FC695", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F3FC695" ], "product": "potassium transporter TrkG" } }, "id": "LMJBGEANGM_141", "locus": "GCA013368015_00101" }, { "type": "cds", "sequence": "contig_1", "start": 61212, "stop": 61385, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTAAGATTTGGAATATTTCATACACTAAGTGCAATAAATAATGCTGGATTAGATATAATTGGCACAAAAAGTTTGCAACCTTATTATCATAATTATTACTTTTTATTTTTAACATCAATTATATTTCTAATTGGTGGAATGGGATTCCCTGTATTCTATGATGTTTGA", "aa": "MSLRFGIFHTLSAINNAGLDIIGTKSLQPYYHNYYFLFLTSIIFLIGGMGFPVFYDV", "aa_hexdigest": "aa88d27af245d632b2199d2d319e902d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6498.521599999998, "isoelectric_point": 6.68991527557373 }, "id": "LMJBGEANGM_142", "locus": "GCA013368015_00102" }, { "type": "cds", "sequence": "contig_1", "start": 61437, "stop": 61820, "strand": "-", "frame": 1, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9", "UniRef:UniRef90_UPI001F33CDF9" ], "nt": "TTGCTATTAGCAGCCTCATCATTTACAACAACCGGTTTAAGTGGAGTTGCCGTTAGTGAAGTATTTAACATGTTTGGACAGATATTAATAGCTGTCTGTTTTATTCTTGGCGGAATTGGAATTTTTACATTTAAACTTTATATTTTTCAATCATTACTTGGATTAAGAACGAATTTATTTAGTGGCCAAATTCACCAAACTGAAAGAGGCGGTTCTAATATTTATGAAACAAAAAAAATGATAAAAGTTTCAATAAGTTTTTTAATTATTATTTCTATATTATTTTCTTTAATATTTTCTTTAATATTTTATTATGATAATACAGCACTTTTTAGTGATGAAAAGATGTCTCAATTAAATGCATTAAGTAAAAATAATCCATGA", "aa": "MLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNP", "aa_hexdigest": "df4b2a49f6c7991871a4e907aea3c07b", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 61136, "stop": 61877, "observations": { "insertion": [ 61208, 61233, 61279, 61313 ], "mutation": [ 61214, 61439, 61868 ], "stop": [ 61214, 61439, 61868 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33CDF9", "query_cov": 1.0, "subject_cov": 0.5059760956175299, "identity": 0.992, "score": 215.0, "evalue": 1.26e-68, "gene_start": 1, "gene_end": 127, "reference_start": 20, "reference_end": 146, "reference_sequence": "MLYWEVSHNPSKHISYLDALLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNPWTFNPYEESIRNPYHNFGMSLRFGIFHTLSAINNAGLDIIGTKVCNLIIIIITFIFNINYISNWWNGIPVFYDVWQKLKASRKTILVIALHYLQNLQLLHTFLHH" }, "paralog": false, "identity": 0.9877551020408163, "score": 430.0, "evalue": 1.22e-155, "description": "Internal stop codon at 61214, 61439, 61868. Frameshift due to insertion around 61208, 61233, 61279, 61313. Nonsense mutation around 61214, 61439, 61868.", "psc": { "uniref90_id": "UniRef90_UPI001F33CDF9", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33CDF9", "UniRef:UniRef50_UPI001F33CDF9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9" ], "product": "potassium transporter TrkG" } }, "id": "LMJBGEANGM_143", "locus": "GCA013368015_00103" }, { "type": "cds", "sequence": "contig_1", "start": 62148, "stop": 62840, "strand": "+", "frame": 3, "gene": "trkA", "product": "Potassium uptake protein KtrA", "db_xrefs": [ "COG:COG0569", "COG:PT", "RefSeq:WP_012855311.1", "SO:0001217", "UniParc:UPI0001BC203B", "UniRef:UniRef100_D1J7H4", "UniRef:UniRef50_D1J7H4", "UniRef:UniRef90_D1J7H4" ], "nt": "ATGAGTTTATTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGTCGGTAGCAAGAACATTATTGGCTGATAGAAATAATAATATAAGGATAGTTTTAGTTGATGAAGATGAAAAACATTTGTTGCCTTTTAAAGATGAAGTAGAAATGATCTATGTTGCCGATTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCCGTTATTGTTGGCGCTTCAAATAATATTGAAATAGTCGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGAGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGTCCTGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGTGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGCCAAATTGAAGATATTATTCAAGTTATTGAAATTTGTGCAATGAATGAAAAATAA", "aa": "MSLFKKIREICIIGIGRYGSSVARTLLADRNNNIRIVLVDEDEKHLLPFKDEVEMIYVADCAEQKTLESLNIKDFDAVIVGASNNIEIVAALTECGVKTIIARASTKRHAQVLKQIGVSSIVCPEEEAGKKTAMLVANPALALYSENMVELQDGFVSASIYVKNPAVANKKISELNFRNKYNVLVTMIKRDKHSYIPEGDFEIKLDDLITLIGQIEDIIQVIEICAMNEK", "aa_hexdigest": "18d450a60381e5c4899d963182ccef7d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC203B", "ncbi_nrp_id": "WP_012855311.1", "uniref100_id": "UniRef100_D1J7H4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC203B", "RefSeq:WP_012855311.1", "UniRef:UniRef100_D1J7H4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7H4", "uniref90_id": "UniRef90_D1J7H4" }, "psc": { "uniref90_id": "UniRef90_D1J7H4", "gene": "TrkA", "product": "Potassium uptake protein KtrA", "uniref50_id": "UniRef50_D1J7H4", "cog_id": "COG0569", "cog_category": "PT" }, "pscc": { "uniref50_id": "UniRef50_D1J7H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H4" ], "product": "Potassium uptake protein KtrA" }, "genes": [ "ktrA", "trkA" ], "id": "LMJBGEANGM_144", "locus": "GCA013368015_00104" }, { "type": "cds", "sequence": "contig_1", "start": 62951, "stop": 63160, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAAGCTTCATCAACAAATAATCAAGTTGTAAGAAAACACGATTTTCAATTTTCTGTTGAAGTAAAACCCGGTTCAATAACCAACCAAAAACATTCAGGTAGATGTTGA", "aa": "MAILDNELLKKLEAKYESNLNNKIIENAIFKNGIKASSTNNQVVRKHDFQFSVEVKPGSITNQKHSGRC", "aa_hexdigest": "3609b43cf55c2d29729f13c1fda61b8a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7802.815200000001, "isoelectric_point": 9.463910484313967 }, "id": "LMJBGEANGM_145", "locus": "GCA013368015_00105" }, { "type": "cds", "sequence": "contig_1", "start": 63197, "stop": 63289, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTAGAAAAAGCAAATGCATATTTAACATGA", "aa": "MKDLNIEDLEFSQNFIHFYDKLEKANAYLT", "aa_hexdigest": "189e783e2c094f0c78409ad88256c469", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3651.0591000000004, "isoelectric_point": 4.581245613098145 }, "id": "LMJBGEANGM_146", "locus": "GCA013368015_00106" }, { "type": "cds", "sequence": "contig_1", "start": 63305, "stop": 63484, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAATCCTATTTCAGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAAACGATATGGTAGAACAAATTAATTGA", "aa": "MELDNEDRLFRRYNENPISDGGYFEFFLNIASKYGIVPKSAMPESFHSEATNDMVEQIN", "aa_hexdigest": "42c67e7c28df1faf8ac2490492c4e0f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6878.514100000002, "isoelectric_point": 4.405101585388183 }, "id": "LMJBGEANGM_147", "locus": "GCA013368015_00107" }, { "type": "cds", "sequence": "contig_1", "start": 63509, "stop": 63898, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTAGAAGCAATTCAACAACATGCTTTGGAAGACGTTTACAACATTTTAGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGACAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTACCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCAATTAAACAATTGCAATCTGGTCAATCAATTTGA", "aa": "MREAYKKNHNMSEVEAIQQHALEDVYNILVKSLGMPPKTFIFEYYDKDKKFVRLPEMTPIKFFETYIKQFVENKVDVISDPREIYPYYRQLVAPSVFNSVGGIEYKMLNIPMNELKAAAIKQLQSGQSI", "aa_hexdigest": "86e947b32f4a59983984df1705be817d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15117.370899999984, "isoelectric_point": 7.850975990295411 }, "id": "LMJBGEANGM_148", "locus": "GCA013368015_00108" }, { "type": "cds", "sequence": "contig_1", "start": 64181, "stop": 64282, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGTGAAGAATAA", "aa": "MLIVDKEFLSKEAIEGLSKEAIEISPYDPLCEE", "aa_hexdigest": "eb71dcd39b353ef260716d4fc5eb2555", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3740.2521000000006, "isoelectric_point": 4.216737174987793 }, "id": "LMJBGEANGM_149", "locus": "GCA013368015_00109" }, { "type": "cds", "sequence": "contig_1", "start": 64396, "stop": 64539, "strand": "+", "frame": 1, "gene": "acpS", "product": "Holo-[acyl-carrier-protein] synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "SO:0001217", "UniRef:UniRef50_D1J7H8", "UniRef:UniRef90_D1J7H8" ], "nt": "ATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTCTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACTAGATGA", "aa": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATR", "aa_hexdigest": "c6a974de7e7b90d325ed84c9ef20712f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "acpS" ], "truncated": "3-prime", "pseudogene": { "start": 64396, "stop": 64723, "observations": { "insertion": [ 64687 ], "mutation": [ 64537 ], "stop": [ 64537 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H8", "query_cov": 1.0, "subject_cov": 0.42727272727272725, "identity": 1.0, "score": 94.7, "evalue": 3.38e-24, "gene_start": 1, "gene_end": 47, "reference_start": 1, "reference_end": 47, "reference_sequence": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATRWAIKEAIFKADNQYYSFNQINITKDEVGRYVFKDFEISTSNEGIYIIAVVLKDRTNSRSYLWI" }, "paralog": false, "identity": 0.9907407407407407, "score": 203.0, "evalue": 1.75e-69, "description": "Internal stop codon at 64537. Frameshift due to insertion around 64687. Nonsense mutation around 64537.", "psc": { "uniref90_id": "UniRef90_D1J7H8", "gene": "AcpS", "product": "Holo-[acyl-carrier-protein] synthase", "uniref50_id": "UniRef50_D1J7H8", "cog_id": "COG0736", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H8", "UniRef:UniRef50_D1J7H8", "COG:COG0736", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "LMJBGEANGM_150", "locus": "GCA013368015_00110" }, { "type": "cds", "sequence": "contig_1", "start": 64720, "stop": 64983, "strand": "+", "frame": 1, "gene": null, "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F", "UniRef:UniRef90_UPI0023AB411F" ], "nt": "ATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGTGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTTAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTCCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGA", "aa": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIM", "aa_hexdigest": "e4346338aaa50bbc249342aa214d6162", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 64720, "stop": 65230, "observations": { "insertion": [ 65173 ], "mutation": [ 64981, 64993 ], "stop": [ 64981, 64993 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AB411F", "query_cov": 1.0, "subject_cov": 0.514792899408284, "identity": 1.0, "score": 182.0, "evalue": 2.14e-57, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIMWSALWNHRDGSKQSKLVNFVARKEITKKKMVKRIADLGQTYYIDTQNPREALATLKDFGQFIKKINHAALFLLKEQEQKMEN" }, "paralog": false, "identity": 0.9822485207100592, "score": 330.0, "evalue": 5.35e-118, "description": "Internal stop codon at 64981, 64993. Frameshift due to insertion around 65173. Nonsense mutation around 64981, 64993.", "psc": { "uniref90_id": "UniRef90_UPI0023AB411F", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AB411F", "UniRef:UniRef50_UPI0023AB411F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F" ], "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "LMJBGEANGM_151", "locus": "GCA013368015_00111" }, { "type": "cds", "sequence": "contig_1", "start": 65026, "stop": 65325, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAATTTCGTTGCAAGAAAAGAAATAACAAAAAAGAAAATGGTTAAAAGAATAGCAGATTTAGGTCAAACTTATTATATTGATACTCAAAACCCTAGAGAAGCATTAGCAACCTTGAAAGATTTTGGCCAATTTATTAAAAGAAATAAATCATGCGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGTGAATTTCATAGTGGCGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAACGCATTGGATTGA", "aa": "MVNFVARKEITKKKMVKRIADLGQTYYIDTQNPREALATLKDFGQFIKRNKSCGVIFAEGTRTKDGKLGEFHSGAFKLAQSSYLTIVPATINNAANALD", "aa_hexdigest": "15320358c81252547587e75baa27fe81", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10985.549299999984, "isoelectric_point": 9.760980796813964 }, "id": "LMJBGEANGM_152", "locus": "GCA013368015_00112" }, { "type": "cds", "sequence": "contig_1", "start": 65371, "stop": 65529, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C", "UniRef:UniRef90_UPI001F16AA7C" ], "nt": "ATGAAGCCAATTCAATTTCAATCACTTGATAGCCGCGTATTAGCAGAACAAGTTAAATCAATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAATGTAAAAGAAGTTAAGAGATAA", "aa": "MKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDNVKEVKR", "aa_hexdigest": "0098145e8ba22746e835912ee04130fd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 65317, "stop": 65529, "observations": { "mutation": [ 65323 ], "stop": [ 65323 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16AA7C", "query_cov": 1.0, "subject_cov": 0.7428571428571429, "identity": 0.981, "score": 94.4, "evalue": 1.97e-24, "gene_start": 1, "gene_end": 52, "reference_start": 19, "reference_end": 70, "reference_sequence": "MDWNRKEKLEVKVTFHKPMKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDKVKEVKR" }, "paralog": false, "identity": 0.9571428571428572, "score": 132.0, "evalue": 5.89e-42, "description": "Internal stop codon at 65323. Nonsense mutation around 65323.", "psc": { "uniref90_id": "UniRef90_UPI001F16AA7C", "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16AA7C", "UniRef:UniRef50_UPI001F16AA7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6043.815600000001, "isoelectric_point": 9.454562568664553 }, "id": "LMJBGEANGM_153", "locus": "GCA013368015_00113" }, { "type": "cds", "sequence": "contig_1", "start": 65533, "stop": 66357, "strand": "+", "frame": 1, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "COG:COG1354", "COG:L", "KEGG:K05896", "RefSeq:WP_020002451.1", "SO:0001217", "UniParc:UPI0003713E10", "UniRef:UniRef100_UPI0003713E10", "UniRef:UniRef50_N9U9Z0", "UniRef:UniRef90_D1J7I0" ], "nt": "ATGTCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACTAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCGCTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCGCATTTATTACAATTAAAAGCTAGAATGGTATTGCAAGACCCAGAAGTTGAAGAAGAAATTAAACAAGAAAAAAAGAGATTGCTTGAACAGATTGCCGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCAAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCGATTCTTGATGGGCATTCCAATAGTTCAAAATTAGTTGTAACTTTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCCGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTAACCTTTAAAGATGTTTTTTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCGGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGCGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAA", "aa": "MSDLEKPIIREISIKEEPNKDQNNQTNSSIFKEKHVFKLKNFDGPLDLLVSLIKEKNISIFDVDLSELANQYLEIIHSLQSYEVDIASEYLVMAAHLLQLKARMVLQDPEVEEEIKQEKKRLLEQIAEYEKFKEISLLLREQEEKRSRLFSKEPEDTQDFVRDIDGSILDGHSNSSKLVVTLRKMFERTYAELIRNITISTIAVSPEEQKERIIKLFKTKLQVTFKDVFSVPSLGHFVITLLAALDLVRQQIIEIYQEENEGEIHFKKGALYEE", "aa_hexdigest": "b496f58686bbbb029ae8479c24047e53", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0003713E10", "ncbi_nrp_id": "WP_020002451.1", "uniref100_id": "UniRef100_UPI0003713E10", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003713E10", "RefSeq:WP_020002451.1", "UniRef:UniRef100_UPI0003713E10" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003713E10", "uniref90_id": "UniRef90_D1J7I0" }, "psc": { "uniref90_id": "UniRef90_D1J7I0", "gene": "ScpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_N9U9Z0", "cog_id": "COG1354", "cog_category": "L", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_N9U9Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9Z0" ], "product": "Segregation and condensation protein A" }, "genes": [ "scpA" ], "id": "LMJBGEANGM_154", "locus": "GCA013368015_00114" }, { "type": "cds", "sequence": "contig_1", "start": 66347, "stop": 66979, "strand": "+", "frame": 2, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_012855318.1", "SO:0001217", "UniParc:UPI0001BC2042", "UniRef:UniRef100_D1J7I1", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_D1J7I1" ], "nt": "ATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTAAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTTAAAACATACTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGTGTCAAAGATTATATTAGCGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCTGCAATTGAAGTTGCGGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGCGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAGGTTGGAGTTGCTCAAACTCCAGGCAACCCCATTTTATATGGAATTACTAATAAATTCTATGATTATTTCAAAATAAAGACCTTAGGAGAATTGCCTCCTTTTCCTGAATTTAATAAATATAGCAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACCAGAAACGGACAATGATGGAGTTTTAATTTCTAATGAGGGGGCAGAATATGATAAATTTTAA", "aa": "MKNKIIEALLFIQGSEGLSSEQAQQVFKLNTIQEARRLLRDFKTYFNNLERGIKVLEFNDVFKFLTIESVKDYISDLVTITRKQRLSNAAIEVAGIVAYKQPITRGMISNIRGVVSDGIVSSLLEKGIIEEVGVAQTPGNPILYGITNKFYDYFKIKTLGELPPFPEFNKYSSEEEEEIDKDFNLFDSQRPETDNDGVLISNEGAEYDKF", "aa_hexdigest": "845b289f2f540fb7362f74354c40e040", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2042", "ncbi_nrp_id": "WP_012855318.1", "uniref100_id": "UniRef100_D1J7I1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2042", "RefSeq:WP_012855318.1", "UniRef:UniRef100_D1J7I1" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I1", "uniref90_id": "UniRef90_D1J7I1" }, "psc": { "uniref90_id": "UniRef90_D1J7I1", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "LMJBGEANGM_155", "locus": "GCA013368015_00115" }, { "type": "cds", "sequence": "contig_1", "start": 66966, "stop": 67799, "strand": "+", "frame": 3, "gene": "rluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "db_xrefs": [ "COG:COG0564", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXK9", "UniRef:UniRef90_D1J7I2" ], "nt": "ATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATACTTGGTAAAGGTTTGCGATAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATACACGGATAAATGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACTTCTAAACATATTGACAACAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATTGACAAGGCACCAAATATTGCAATGCATTCTGAAGAAAATTGTCTAGATGATCAAGTTTTAAAATATTTAAATTTTAAAAAAGTTTCTTCGTTTAGACCTGCTTCAATTGGCAGAATAGATAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAACAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATGTTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGAGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGTAAAAAGCATCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTACATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGA", "aa": "MINFKAKKDDEGRILFKYLVKVCDNVPISRIERLFREKDIKVNNTRINDKQYKVKEGDLISIYGLIETSKHIDNKANTNINFKVIYEDKNLLIIDKAPNIAMHSEENCLDDQVLKYLNFKKVSSFRPASIGRIDKKTSGLVVYAKNYKTLVEFENKQNKFDKIYQFVSDFDEDVLDITVRLKKDIKNEKMIVDNNFGVKAQTIFYKEGNRKFAKIITGKKHQIRATLAYLKTPILGDIKYGGKFAKRMYLHSYSIKFHDLSDDFKEYNEQVFICYPK", "aa_hexdigest": "1b210bd9ea32a378ac05b077873cf6af", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_D1J7I2", "query_cov": 1.0, "subject_cov": 0.9964028776978417, "identity": 0.993, "score": 545.0, "evalue": 4.56e-196, "valid": true, "gene": "RluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "uniref50_id": "UniRef50_A0A448ZXK9", "cog_id": "COG0564", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXK9" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [ "rluA" ], "id": "LMJBGEANGM_156", "locus": "GCA013368015_00116" }, { "type": "cds", "sequence": "contig_1", "start": 67822, "stop": 68124, "strand": "+", "frame": 1, "gene": "gatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "db_xrefs": [ "COG:COG0721", "COG:J", "RefSeq:WP_012855320.1", "SO:0001217", "UniParc:UPI0001BC2044", "UniRef:UniRef100_D1J7I3", "UniRef:UniRef50_D1J7I3", "UniRef:UniRef90_D1J7I3" ], "nt": "ATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTGCTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCATATCAATGAATCACCCATTTGTTTCGAACAACTAAGAAGTGATGAACCGGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTATGTAATTATGAAGAAGGTAATAAATGATAAATAA", "aa": "MEKEEIKKLANDLLFEPSEEVIKLTNNLLNSIEKGLKDLEEFDLQDIKPMSHINESPICFEQLRSDEPDKSFYLNKQDLLNNASEHDENYVIMKKVINDK", "aa_hexdigest": "e5f45cfef76f2cb5e32c174fb203ef47", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2044", "ncbi_nrp_id": "WP_012855320.1", "uniref100_id": "UniRef100_D1J7I3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2044", "RefSeq:WP_012855320.1", "UniRef:UniRef100_D1J7I3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I3", "uniref90_id": "UniRef90_D1J7I3" }, "psc": { "uniref90_id": "UniRef90_D1J7I3", "gene": "GatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "uniref50_id": "UniRef50_D1J7I3", "cog_id": "COG0721", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I3" ], "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C" }, "genes": [ "gatC" ], "id": "LMJBGEANGM_157", "locus": "GCA013368015_00117" }, { "type": "cds", "sequence": "contig_1", "start": 68114, "stop": 69016, "strand": "+", "frame": 2, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A449AI30", "UniRef:UniRef90_A0A6A8PZD5" ], "nt": "ATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCCTTTGTTTTTGAGGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAGGACAATTATGCAGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTCGCAAGAACAAATTTGGACGAATTTGGTATGGGCGGTAGTGGAGAATATTCATGCAACGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGAGGTTCTTCATCAGGTGCCGCTGCAACTTTTTCTAAAAATATCTCGTTTGCAATTGGATCGGATACAGGCGATTCAGTTAGAAAACCCGCATCAAATGTTGGCGTGGTTGGATTTAAACCAAGTTATGGCGCCATAAGCCGTTATGGATTGTCTGCTTTTGCTTCATCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAACTCTATCATCAGTATTGTTTAAGCAAGATTTAAAACATGATTTGACATCAGTTGATTTGTCGTTTAATGCAGATAGTGTTATTGAAACAAAACCACACAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTTGTAAAAATTTCAATAAATGAAAAGTTGCTGCAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCTTCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACTGGCAATTTTTGA", "aa": "MINKGNLKEAILQLNADKNNAVSFVFEDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNVGVVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNF", "aa_hexdigest": "0cdccdbe7bc29cc7f4bb22843046183d", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 68114, "stop": 69319, "observations": { "mutation": [ 69014 ], "stop": [ 69014 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PZD5", "query_cov": 0.9966666666666667, "subject_cov": 0.6764705882352942, "identity": 0.99, "score": 564.0, "evalue": 1.56e-200, "gene_start": 1, "gene_end": 299, "reference_start": 1, "reference_end": 299, "reference_sequence": "MINKGNLKEAILQLNADKNNAVSFVFKDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGQVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNSWEETFNLSRTEGIGKMVQSRMILGSYFLEDNNQIKYFVRAKKMRRYLSNYFTNVHKSADIFIYPASNMSAPLIGKLYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE" }, "paralog": false, "identity": 0.9800498753117207, "score": 768.0, "evalue": 2.5e-283, "description": "Internal stop codon at 69014. Nonsense mutation around 69014.", "psc": { "uniref90_id": "UniRef90_A0A6A8PZD5", "gene": "GatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "uniref50_id": "UniRef50_A0A449AI30", "cog_id": "COG0154", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PZD5", "UniRef:UniRef50_A0A449AI30", "COG:COG0154", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AI30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI30" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A" } }, "id": "LMJBGEANGM_158", "locus": "GCA013368015_00118" }, { "type": "cds", "sequence": "contig_1", "start": 69059, "stop": 69442, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "ATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTAAGTGATTATTTCACTAATGTTCATAAAAGCGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATCATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACAGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTGGCAATTGACGGGGAAATATATAAAGATTGCAAGATGCTAAGTCATGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAA", "aa": "MVQSRMILGSYFLEDNNQIKYFVKAKKMRRYLSDYFTNVHKSADIFIYPASNMSAPLIGKSYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE", "aa_hexdigest": "f1825d3a44d9743c382b929c3189e9c7", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 119, "aa_cov": 0.8661417322834646, "hmm_cov": 0.9915966386554622, "evalue": 1.6484559782715152e-10, "score": 36.06938552856445, "start": 2, "stop": 111 } ], "seq_stats": { "molecular_weight": 14632.675399999984, "isoelectric_point": 7.782832908630372 }, "id": "LMJBGEANGM_159", "locus": "GCA013368015_00119" }, { "type": "cds", "sequence": "contig_1", "start": 69501, "stop": 69779, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGTTTTCTCCAATTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCTGGAAGTTTGCCTCTTTTAAATTCTAGTGCCATAATTAAGGCAATTAAATTAGCCAAGGCGCTAGAAATGGAAATAGATCCATTAGTTAGATTTGACAGGAAAAATTATTTTTATCCCGATTTAACAAAAGGATATCAAATTACTCAACAATTTCATCCGATTGGTAAAAATGGTTTGATAAGGGCAAAGGTTCAAAATTCATGA", "aa": "MFSPIQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNS", "aa_hexdigest": "d10df95cb2bcd46dd464c4c4e5d60deb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 90, "aa_cov": 0.9782608695652174, "hmm_cov": 1.0, "evalue": 7.905135710950624e-33, "score": 109.30573272705078, "start": 1, "stop": 90 } ], "seq_stats": { "molecular_weight": 10358.729099999991, "isoelectric_point": 7.964891624450684 }, "id": "LMJBGEANGM_160", "locus": "GCA013368015_00120" }, { "type": "cds", "sequence": "contig_1", "start": 69807, "stop": 70856, "strand": "+", "frame": 3, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.6", "EC:6.3.5.7", "GO:0016884", "KEGG:K02434", "SO:0001217", "UniRef:UniRef50_A0A449AA70", "UniRef:UniRef90_D1J7F4" ], "nt": "ATGGAAGAAGATACTGCTAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTTCCTTTAATTGAAATAGTTTCTAATCCTGTAATGCACAGCGCAGAGGATGCGATGGCTTACGTTGAAGCTATAAGACAAACAGCATTAGTTTTAGATATTAGTGATGCAAAATTAAATGAAGGAAGTTTAAGAACTGACGTTAATTTATCAGTAAGAAGAAAGGGAGAAAATGTCCTTAATACTCGCACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCAAAAGGTGATGCAATTAGTTACAAATATTTTCCAGATCCGAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATCAAAAAGAATTAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTGTTGAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCAAGAAAGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAA", "aa": "MEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENVLNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLSFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE", "aa_hexdigest": "bfc901fa3d2a1a8f540d34b6c39ead54", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatB" ], "truncated": "5-prime", "pseudogene": { "start": 69507, "stop": 70856, "observations": { "mutation": [ 69777 ], "stop": [ 69777 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F4", "query_cov": 1.0, "subject_cov": 0.7378435517970402, "identity": 0.997, "score": 662.0, "evalue": 7.48e-238, "gene_start": 1, "gene_end": 349, "reference_start": 125, "reference_end": 473, "reference_sequence": "MNNDWEIVIGIEIHIELNTKTKMFSPIQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNSWKTFSIERIHMEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENVLNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLLFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE" }, "paralog": false, "identity": 0.9955456570155902, "score": 879.0, "evalue": 0.0, "description": "Internal stop codon at 69777. Nonsense mutation around 69777.", "psc": { "uniref90_id": "UniRef90_D1J7F4", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.6", "6.3.5.7" ], "uniref50_id": "UniRef50_A0A449AA70", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0016884" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F4", "EC:6.3.5.6", "EC:6.3.5.7", "UniRef:UniRef50_A0A449AA70", "COG:COG0064", "COG:J", "KEGG:K02434", "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AA70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AA70" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" } }, "id": "LMJBGEANGM_161", "locus": "GCA013368015_00121" }, { "type": "cds", "sequence": "contig_1", "start": 71131, "stop": 71286, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGCTCGGTTGATGAAAAGTATGAAGTGGTGCTAAAGAATGTAATTTTGGGTCAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACGTTATGA", "aa": "MYLFLSFIPISILMLGIIGSVDEKYEVVLKNVILGQIIPGISEVIPSFSTL", "aa_hexdigest": "69d05ef375dc0ac833d6cb7a1bce4907", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5611.733599999998, "isoelectric_point": 4.406579399108887 }, "id": "LMJBGEANGM_162", "locus": "GCA013368015_00122" }, { "type": "cds", "sequence": "contig_1", "start": 71235, "stop": 71441, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTTTAATTCTCGATTTTCACACTCTGAATGGAGATTTGTGTTCATATAAAGCATCTATTGAAAAAATAAATTTTGAATATCCCTTCGATGCTAAAATTAATACTGATAGTGCAAATAAGATTAAAGCAATTGCATTGCCTGTAGAACCTCATAACGTACTAAATGATGGAATTACTTCTGAAATTCCGGGAATAATTTGA", "aa": "MNPLILDFHTLNGDLCSYKASIEKINFEYPFDAKINTDSANKIKAIALPVEPHNVLNDGITSEIPGII", "aa_hexdigest": "c2959e663757208aed4c1e9c410f89cd", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7498.497200000001, "isoelectric_point": 4.7775674819946286 }, "id": "LMJBGEANGM_163", "locus": "GCA013368015_00123" }, { "type": "cds", "sequence": "contig_1", "start": 71461, "stop": 71607, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTGTCTTTAATTTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGACTTGGAAAATTTGATGCCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGA", "aa": "MILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIY", "aa_hexdigest": "34a95c0f3e2425c725d584f3efb8db5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5488.648999999999, "isoelectric_point": 5.955214500427247 }, "id": "LMJBGEANGM_164", "locus": "GCA013368015_00124" }, { "type": "cds", "sequence": "contig_1", "start": 71620, "stop": 71931, "strand": "+", "frame": 1, "gene": null, "product": "YhjD/YihY/BrkB family envelope integrity protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95", "UniRef:UniRef90_UPI002FE35A95" ], "nt": "TTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCTGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCATTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTACATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGA", "aa": "MFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSF", "aa_hexdigest": "f4d5323e4ec5f05e3006e13736be685c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 71322, "stop": 72011, "observations": { "insertion": [ 71370, 71926, 71939 ], "stop": [ 71416, 71605 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE35A95", "query_cov": 1.0, "subject_cov": 0.42738589211618255, "identity": 0.99, "score": 194.0, "evalue": 7.69e-61, "gene_start": 1, "gene_end": 103, "reference_start": 113, "reference_end": 215, "reference_sequence": "MRFYRQCNCFNLICAIGINFSIEGIFKIYFSIDALYEHKSPFRAWKSRIKGFIVSVFVCLILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIYWFTTILFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSFWKNKNFNLKSFDFFANFSLFIVINDI" }, "paralog": false, "identity": 0.9736842105263158, "score": 417.0, "evalue": 3.57e-151, "description": "Internal stop codon at 71416, 71605. Frameshift due to insertion around 71370, 71926, 71939.", "psc": { "uniref90_id": "UniRef90_UPI002FE35A95", "product": "YhjD/YihY/BrkB family envelope integrity protein", "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE35A95", "UniRef:UniRef50_UPI002FE35A95" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95" ], "product": "YhjD/YihY/BrkB family envelope integrity protein" } }, "id": "LMJBGEANGM_165", "locus": "GCA013368015_00125" }, { "type": "cds", "sequence": "contig_1", "start": 72151, "stop": 72900, "strand": "+", "frame": 1, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4", "UniRef:UniRef90_D1J7I4" ], "nt": "GTGGGTGTTTTGATTGCTATCTCGGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCCATAAGTGCCTTACCAAGTATAAAATTTAGCTTCATTGGTTTGCCGATAAAAATAACTGGTTTTATTTTTGGACCAATTGTTGGTATGATAACTGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGGTTTGTTCCTGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGTAACTCTGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAGCCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTGGGTATTCAAGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTTAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTTTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATTTTATTGGCGTGA", "aa": "MGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLA", "aa_hexdigest": "9db49f7cfba2e9009cbeac8f6e97cb26", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 72052, "stop": 73056, "observations": { "mutation": [ 72091, 72127, 72898 ], "stop": [ 72091, 72127, 72898 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I4", "query_cov": 0.9959839357429718, "subject_cov": 0.7425149700598802, "identity": 0.992, "score": 430.0, "evalue": 3.97e-150, "gene_start": 2, "gene_end": 249, "reference_start": 35, "reference_end": 282, "reference_sequence": "MNKLPGGLRHYIYWARSQTNISYRKWNSKRIAFVGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLAWADTSTLKTDFWVNFTAQSLFSPVKIFFNLAIILATYKVVAPLIKSKEGGHL" }, "paralog": false, "identity": 0.9910179640718563, "score": 624.0, "evalue": 3.75e-229, "description": "Internal stop codon at 72091, 72127, 72898. Nonsense mutation around 72091, 72127, 72898.", "psc": { "uniref90_id": "UniRef90_D1J7I4", "product": "ECF transporter S component", "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I4", "UniRef:UniRef50_D1J7I4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4" ], "product": "ECF transporter S component" } }, "id": "LMJBGEANGM_166", "locus": "GCA013368015_00126" }, { "type": "cds", "sequence": "contig_1", "start": 73056, "stop": 73466, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACCAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACACTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAGTGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGCTACATTGAATTTCAAACAAATTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGCTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACTGAAGAGTTTGTTTCTCAATGA", "aa": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQ", "aa_hexdigest": "7074d2209fe7d3c109b94223dda4f3ea", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15508.084699999981, "isoelectric_point": 6.56259593963623 }, "id": "LMJBGEANGM_167", "locus": "GCA013368015_00127" }, { "type": "cds", "sequence": "contig_1", "start": 73494, "stop": 74411, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311", "UniRef:UniRef90_UPI001E424311" ], "nt": "TTGCTTCAAAGTGAATTTGCCAAATTGCAATATGGTGTTGAAATTGAACTTGGAAGTAAATTAGACGACTTATTAAATGAATTAAATCAATTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTCTTTTTAAGAGATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCAACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAGATTTATAAGCCTTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAATTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATCTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCATTTATTCACTTTTATAAAAATCTATATTTTAATGGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCGTTTGAATTATTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCAAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATGTTACAAGAGAAATTCAAACGTCTAATAACTACGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACAGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGA", "aa": "MLQSEFAKLQYGVEIELGSKLDDLLNELNQFDLRNIDAKNLKFDTLFKRFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKFLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKTVESKYKDAKNEYF", "aa_hexdigest": "54e2ce1e2a41e34926ab58552568f0ce", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 73194, "stop": 74456, "observations": { "mutation": [ 73464, 74409 ], "stop": [ 73464, 74409 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E424311", "query_cov": 1.0, "subject_cov": 0.6475583864118896, "identity": 0.98, "score": 568.0, "evalue": 1.14e-201, "gene_start": 1, "gene_end": 305, "reference_start": 147, "reference_end": 451, "reference_sequence": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQWKPVFQDYKLLLQSEFAKLQYGVEIELGSKLDDLFSELSQFDLRNIDAKNLKFDTLFKKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKFLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKIVESKYKDAKNEYFWNYKTEGNAILKETKSFKKF" }, "paralog": false, "identity": 0.9833333333333333, "score": 820.0, "evalue": 1.77e-303, "description": "Internal stop codon at 73464, 74409. Nonsense mutation around 73464, 74409.", "psc": { "uniref90_id": "UniRef90_UPI001E424311", "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E424311", "UniRef:UniRef50_UPI001E424311" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36425.60540000005, "isoelectric_point": 9.484798240661618 }, "id": "LMJBGEANGM_168", "locus": "GCA013368015_00128" }, { "type": "cds", "sequence": "contig_1", "start": 74532, "stop": 75191, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTACAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGCGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCAAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATTAATAATCCTTCACTTATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGTGAAACATTGAATCAATTGAATAAATTTGTTTTAGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGGAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTACAAAGACGAGAAGACTCAAGAGGATTTAAGCTTTTATATGTCTCAAGAAGACAATATGGAAAATATATACAAATATGAAATAGAGCCATACCACTTTGTTTGA", "aa": "MINLIKIKEFNNSVLNEQVKKIKENLQFLKILRNEFDSYKYVVSDFKTASKKQIKLFVSKINIYKIIAKANIDVNFLVEKLGSLSDDEKIRLECEKILINNPSLIIIGPNISKLPYEKQCETLNQLNKFVLDNHKMAIYFLDNINIAQKITTDLYIINSSKIIEQGKTSQLIRNPINPIVKNMLNYKDEKTQEDLSFYMSQEDNMENIYKYEIEPYHFV", "aa_hexdigest": "2bc275ded7eed0db9286dd284a20a5be", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25769.683600000022, "isoelectric_point": 8.592297935485842 }, "id": "LMJBGEANGM_169", "locus": "GCA013368015_00129" }, { "type": "cds", "sequence": "contig_1", "start": 75252, "stop": 75437, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTATTTATTCCACAAAATACTTTTCAAAGTAAAGGAAATAAAACAATAGACAATAAGGAACCATTCGAAGAAACAACGCTTATAAATTTAATCGATGATGAAGATAACGAACTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGACAGATGCAAAAAATGA", "aa": "MNLFIPQNTFQSKGNKTIDNKEPFEETTLINLIDDEDNELRNLNMEKTFNHKIVEKDRCKK", "aa_hexdigest": "0b4fd8dfa683d205c1bc5b0b2f3587af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7284.136799999998, "isoelectric_point": 5.284571266174315 }, "id": "LMJBGEANGM_170", "locus": "GCA013368015_00130" }, { "type": "cds", "sequence": "contig_1", "start": 75444, "stop": 75605, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTCACCATTATTTTGCCGCCCCCAAATGTAACTGGCAATCTCCATATTGGCCATGCCCTAGACAATTATATTTCTGATACAATAATAAGGTTTAAGAAGTTACAGGGATATGATGTATTGTGA", "aa": "MKIFQTHDESKKPFTIILPPPNVTGNLHIGHALDNYISDTIIRFKKLQGYDVL", "aa_hexdigest": "a7f9fee12811d4164b5043ad99892ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.8867924528301887, "hmm_cov": 1.0, "evalue": 1.5033589001661456e-17, "score": 58.95497131347656, "start": 6, "stop": 52 } ], "seq_stats": { "molecular_weight": 6078.990000000001, "isoelectric_point": 8.123548316955567 }, "id": "LMJBGEANGM_171", "locus": "GCA013368015_00131" }, { "type": "cds", "sequence": "contig_1", "start": 75777, "stop": 75902, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGCCCTTGATTATGGTTTTGAAAGATTCACTTTAGATGATAATTCTAATGAAGCTGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAAAAAGCAATAAATTGA", "aa": "MALDYGFERFTLDDNSNEAVLKVFIELYENGLIYRDKKAIN", "aa_hexdigest": "abc8b3314988d02db92af6055c58a28c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 39, "aa_cov": 0.9512195121951219, "hmm_cov": 1.0, "evalue": 1.734818989577086e-09, "score": 32.398719787597656, "start": 3, "stop": 41 } ], "seq_stats": { "molecular_weight": 4829.396900000001, "isoelectric_point": 4.507070732116699 }, "id": "LMJBGEANGM_172", "locus": "GCA013368015_00132" }, { "type": "cds", "sequence": "contig_1", "start": 75966, "stop": 76448, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTACTACATAAAATATCCAATTAAGAATTCAAAAAACTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTCTCAGATGTAGCTTTAGCAATAAACCCGCAAGATAGTAGAGCAAAACAATTAATAAAAGAAATAATAATACATCCATTAACAAATAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTTCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAATGATTTAGCAGGTGAATTTAAGGGATTAGATCGCTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGATATAGTGAAAGATCAAAGGCCCCAATTGAAATTTTAGTTCAGCCACAATGA", "aa": "MYYIKYPIKNSKNFLEVATTRPETMFSDVALAINPQDSRAKQLIKEIIIHPLTNKEIPIILSNKIDPNFGSGIMKVSAHAVDDIEIIIENKLPINECINKDGKLNDLAGEFKGLDRFEAREKIAHLLSQKGLLIKEEEVVSNIGYSERSKAPIEILVQPQ", "aa_hexdigest": "72c9fd519ff81e9392659af94b28fce3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 60, "aa_cov": 0.36875, "hmm_cov": 1.0, "evalue": 3.0882431668705785e-11, "score": 38.63521957397461, "start": 47, "stop": 105 } ], "seq_stats": { "molecular_weight": 18039.706699999984, "isoelectric_point": 5.959647941589356 }, "id": "LMJBGEANGM_173", "locus": "GCA013368015_00133" }, { "type": "cds", "sequence": "contig_1", "start": 76830, "stop": 77084, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTCTTTAGCTTGTATTTTATGGATCAAAAACCTTTTGAACATCTCTTATTGCATGGATTAGTTAGAGATTCACAAGGAAGAAAAATGTCTAAATCGCTTGGCAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGTACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGTGCTAAACTATTTGCTAATAAATTCTGA", "aa": "MYFFSLYFMDQKPFEHLLLHGLVRDSQGRKMSKSLGNGINPIQVIDEYGSDVLRMSLIFNCTPGLDINYSDEKIQGAKLFANKF", "aa_hexdigest": "2c3b7d7103de409c769653338b70449a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.9642857142857143, "subject_cov": 0.9310344827586207, "identity": 0.58, "score": 106.0, "evalue": 1.57e-28, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 69, "aa_cov": 0.8214285714285714, "hmm_cov": 0.9855072463768116, "evalue": 3.2283464903753124e-22, "score": 74.33128356933594, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 9653.03739999999, "isoelectric_point": 6.705830192565918 }, "id": "LMJBGEANGM_174", "locus": "GCA013368015_00134" }, { "type": "cds", "sequence": "contig_1", "start": 77370, "stop": 77903, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTATATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCTTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATACAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCGGTATAGATTTAAAAATAATTAACAAATTAAGCAATTTTTCTTGGAAAGAAAATCATGACTTTTCACTAAAAACAGAAAGTTTTGAAGTATTTATAATTCAAAGCAAGCAAGACAAGGAAAATGAAATTCTAGAATTAAATAAATTAATTGAATTTAGTCAAAAAGAAATTGAATTCAATGAAAAATTAATTAATAATCCTAAATTTATGGAAAAGGCTAATAAAAATACAATCAATGAAAAATTAGCAAATTTAAAATTACATAAAGAAAAATTAGAGTTTTATAAAAACCAACTTAAAGAAAAACAAGAAAAAATAAATTAA", "aa": "MPFLTDYVFEKLYHEELLCSNLQTFALNKESSNNTPKIISLITALRKYREDKKISKSIELFFYLNNLVFSGIDLKIINKLSNFSWKENHDFSLKTESFEVFIIQSKQDKENEILELNKLIEFSQKEIEFNEKLINNPKFMEKANKNTINEKLANLKLHKEKLEFYKNQLKEKQEKIN", "aa_hexdigest": "a3d12a7f50df3779bf133bd4737fe2a1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21179.149100000017, "isoelectric_point": 8.428999519348146 }, "id": "LMJBGEANGM_175", "locus": "GCA013368015_00135" }, { "type": "cds", "sequence": "contig_1", "start": 77984, "stop": 78208, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "GTGACTGAATTCAGTAACTTTGAAGCTGGAACAAAAGGAATTGCAACCGCTATTGCTCAAAATAGAGATTGCTATTCAGTAGTTGGCGGAGGCGACTCTGTTACAGCAATAAACAAATTAGGTTATCAAGATAAATTTAGTTTTATTTCAACTGGCGGTGGAGCATCAATAGAATTTGTCCAAGGAAAGAAATTACCTGGAATAGAAGCAATTCAAGACAAATAA", "aa": "MTEFSNFEAGTKGIATAIAQNRDCYSVVGGGDSVTAINKLGYQDKFSFISTGGGASIEFVQGKKLPGIEAIQDK", "aa_hexdigest": "3ab3b07e362735e0cc9e197cf2aaa980", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030BBA936", "query_cov": 0.972972972972973, "subject_cov": 0.8470588235294118, "identity": 0.542, "score": 84.3, "evalue": 6.8e-20, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A166IH01", "kegg_orthology_id": "K00927" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 64, "aa_cov": 0.8513513513513513, "hmm_cov": 1.0, "evalue": 4.3145798832984893e-23, "score": 77.40230560302734, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 7736.569799999998, "isoelectric_point": 5.1351415634155275 }, "id": "LMJBGEANGM_176", "locus": "GCA013368015_00136" }, { "type": "cds", "sequence": "contig_1", "start": 78224, "stop": 78748, "strand": "-", "frame": 2, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "SO:0001217", "UniRef:UniRef50_P62415", "UniRef:UniRef90_D1J7I6" ], "nt": "GTGTATGTTAATGATGCTTTTGGAACCGCGCATAGAGCACATGCATCAACATATGGAATTTCACAATATTCTAAGGAATCAGCATTAGGATTTTTAATGAATAATGAAGTTGAACACCTTCAAAAATTATTAGTAGGATTTAAACGTCCTTTTGTTGCAATAATTGGTGGAGCAAAAGTAAGTGATAAAATCAAAGTTCTAGAAAAGTTATTTGAACTAGCAGATAAGGTTTTAATTGTTGGTGGTATGGCATATACATTCCAAAAAGCCTTGAATAGAAAAATTGGAACTTCTATTTTTGAAGAAGATAAAGTTGAAATGGCAAAAGAATATTTAAACAAATATAAAGATAAAATTGTTTTGCCAATTGACAATGCTTATGTAGATGAATTTGCTGACAAAACTCCTAAATTTACAAGTCCAGAAAATCCTGATATTCCTGACGGAAATATGGGATTAGATATTGGACCAGAAAGTATTAAATTATTCGCAAGTATTATTAAAAATGCTAAAACTATCTTTTGA", "aa": "MYVNDAFGTAHRAHASTYGISQYSKESALGFLMNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNRKIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKNAKTIF", "aa_hexdigest": "b74ab664ea2915be8d5274519089bf86", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [ "pgk" ], "truncated": "both", "pseudogene": { "start": 77984, "stop": 79048, "observations": { "mutation": [ 78226, 78766 ], "stop": [ 78226, 78766 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I6", "query_cov": 1.0, "subject_cov": 0.43828715365239296, "identity": 0.9940000000000001, "score": 340.0, "evalue": 4.24e-115, "gene_start": 1, "gene_end": 174, "reference_start": 144, "reference_end": 317, "reference_sequence": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEDLAKYWASLGDVYVNDAFGTAHRAHASTYGISQYSKESALGFLMNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNRKIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKNAKTIFWNGPAGVTEFSNFEAGTKGIATAIAQNRDCYSVVGGGDSVTAINKLGYQDKFSFISTGGGASIEFVQGKKLPGIEAIQDK" }, "paralog": false, "identity": 0.9943502824858758, "score": 685.0, "evalue": 2.52e-253, "description": "Internal stop codon at 78226, 78766. Nonsense mutation around 78226, 78766.", "psc": { "uniref90_id": "UniRef90_D1J7I6", "gene": "Pgk", "product": "Phosphoglycerate kinase", "uniref50_id": "UniRef50_P62415", "cog_id": "COG0126", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I6", "UniRef:UniRef50_P62415", "COG:COG0126", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_P62415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P62415" ], "product": "Phosphoglycerate kinase" } }, "id": "LMJBGEANGM_177", "locus": "GCA013368015_00137" }, { "type": "cds", "sequence": "contig_1", "start": 78764, "stop": 79177, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAAGAAAACCATTGAAGACCTTCAATTAGAAAACAAAAAGGTTATTTTAAGAGTTGATTTTAACGTTCCTATTTCAAACAACACCATTATGGACACAAAAAGAATTGATGAAGAATTGCCAACAATCAAATACATATTGAGTAAAAATGCTTCTTTAATCATTCTATCTCATTTAGGAAGAGTTAAAACAGAAGAAGACAAGAAAACTAAATCTTTAGAAATTGTAGCTAAAAAATTGGCGCAATTATTAAATAGAGACGTTAAATTTATTAACGAAAATAGAAGTGAAGCAGTTACAAATGCTGCTAAAAACTTAAAACCTGGAGAAATTTTGGTTTTAGAAAATACTCGTTTTCAAGATTTAAATAATAAAGCAGAATCCAATAATGATGAAGATCTTGCAAAATATTGA", "aa": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEDLAKY", "aa_hexdigest": "515c239c6121c3015d4e53237a087b3c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 133, "aa_cov": 0.9635036496350365, "hmm_cov": 0.9548872180451128, "evalue": 2.1482152552145645e-40, "score": 134.35263061523438, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 15706.88729999998, "isoelectric_point": 8.729293251037596 }, "id": "LMJBGEANGM_178", "locus": "GCA013368015_00138" }, { "type": "cds", "sequence": "contig_1", "start": 79289, "stop": 79465, "strand": "+", "frame": 2, "gene": null, "product": "YARHG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4", "UniRef:UniRef90_D1J7I7" ], "nt": "ATGGCAAAAATTTTAAATGCTCAAAAGCAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTTCATTATTTTAAAGAATCTAGATGA", "aa": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESR", "aa_hexdigest": "c5016de7e57661d77b4a08d5e9f9db54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 79289, "stop": 79696, "observations": { "mutation": [ 79463, 79613 ], "stop": [ 79463, 79613 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I7", "query_cov": 1.0, "subject_cov": 0.4264705882352941, "identity": 1.0, "score": 114.0, "evalue": 1.98e-31, "gene_start": 1, "gene_end": 58, "reference_start": 1, "reference_end": 58, "reference_sequence": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESRWMKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9851851851851852, "score": 245.0, "evalue": 1.7e-85, "description": "Internal stop codon at 79463, 79613. Nonsense mutation around 79463, 79613.", "psc": { "uniref90_id": "UniRef90_D1J7I7", "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I7", "UniRef:UniRef50_A0A448ZYF4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4" ], "product": "YARHG domain-containing protein" } }, "id": "LMJBGEANGM_179", "locus": "GCA013368015_00139" }, { "type": "cds", "sequence": "contig_1", "start": 79466, "stop": 79615, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F36E0B6", "UniRef:UniRef90_UPI001F36E0B6" ], "nt": "ATGAAAACAATGGATGAAAAATTGCAAGATCAAGTGCAAGAATTTTCAAAAAGGCAAAAAAAATTAAATGCCAAATATAAAAGCTACATTGAATATTCTAAATGGTCTGAATCGCCATATGCAACAAAAACTAAACCGCTTACAAAATGA", "aa": "MKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTK", "aa_hexdigest": "78f0925452087919a2505a5dae3b3c70", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 79430, "stop": 79696, "observations": { "mutation": [ 79463, 79613 ], "stop": [ 79463, 79613 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F36E0B6", "query_cov": 1.0, "subject_cov": 0.5568181818181818, "identity": 0.9390000000000001, "score": 85.1, "evalue": 1.29e-20, "gene_start": 1, "gene_end": 49, "reference_start": 13, "reference_end": 60, "reference_sequence": "MEKFHYFKESRWMKTMDEKLQDQVQEFSKGKKLNAKYKSYIEYSKWSESPYAAKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9431818181818182, "score": 142.0, "evalue": 7.77e-46, "description": "Internal stop codon at 79463, 79613. Nonsense mutation around 79463, 79613.", "psc": { "uniref90_id": "UniRef90_UPI001F36E0B6", "uniref50_id": "UniRef50_UPI001F36E0B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F36E0B6", "UniRef:UniRef50_UPI001F36E0B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F36E0B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F36E0B6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5933.764700000001, "isoelectric_point": 9.698575401306151 }, "id": "LMJBGEANGM_180", "locus": "GCA013368015_00140" }, { "type": "cds", "sequence": "contig_1", "start": 79839, "stop": 80297, "strand": "+", "frame": 3, "gene": null, "product": "DAC domain-containing protein", "db_xrefs": [ "EC:2.7.7.85", "KEGG:K18672", "SO:0001217", "UniParc:UPI0001BC204C", "UniRef:UniRef100_D1J7I8", "UniRef:UniRef50_D1J7I8", "UniRef:UniRef90_D1J7I8" ], "nt": "TTGGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCCCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATTACTATTGAAAACAAAGATAATCTTGATTTGCTAAGAACTGATGGTGTAATATTAAATGCTAACATTTCTGCTTCTTTAATTATTTCTATTTTTAATAAACACGCTCCACTTCACGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGTGCAAGACACCGTGCTGCTATGGGTATTTCAGAAATTTGCGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGCGGAATTACAATAGCTAAAAACGGAACTTTTAGCCCTGTTTTACTAGAAAATCTACAAGAAAGATTAGTCAATATTTTTAAGGATAATAATTAA", "aa": "MGESTKIRIIYQLKEALEFLSKTKTGAIITIENKDNLDLLRTDGVILNANISASLIISIFNKHAPLHDGAIIIRNNKIYYAATYYKITKKSIDNKYGARHRAAMGISEICDATTIVVSEENGGITIAKNGTFSPVLLENLQERLVNIFKDNN", "aa_hexdigest": "0118841dcdf0adc1110f00f51ad17697", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC204C", "uniref100_id": "UniRef100_D1J7I8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC204C", "UniRef:UniRef100_D1J7I8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I8", "uniref90_id": "UniRef90_D1J7I8" }, "psc": { "uniref90_id": "UniRef90_D1J7I8", "product": "DAC domain-containing protein", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_D1J7I8", "kegg_orthology_id": "K18672" }, "pscc": { "uniref50_id": "UniRef50_D1J7I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I8" ], "product": "DAC domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_181", "locus": "GCA013368015_00141" }, { "type": "cds", "sequence": "contig_1", "start": 80871, "stop": 82064, "strand": "+", "frame": 3, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "RefSeq:WP_012855329.1", "SO:0001217", "UniParc:UPI0000129DB6", "UniRef:UniRef100_P22679", "UniRef:UniRef50_Q8Y422", "UniRef:UniRef90_P22679" ], "nt": "ATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTACGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCTGACTATGTTAAAAACATGATTACCGGTGCCGCCCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATAGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGATGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGATGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATACGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATTACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTAAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACACACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTGACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGGAGAACTGTTGGTGCCGGCTCAGTTACAAAAATTCTTAAATAA", "aa": "MAKLDFDRSKPHVNIGTIGHVDHGKTTLTAAIATVLAKKGLAEARDYASIDNAPEEKARGITINTSHIEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPKIVVFLNKIDMFKDDEREEMVGLVEMDVRSLLSEYGFDGDNAPIIAGSALKALQGDPEYEKGILELMDAVDTYIEEPKRETDKPFLMAVEDVFTITGRGTVATGRVERGVLQLNEEVEIVGLKPTKKTVVTGIEMFRKNLKEAQAGDNAGLLLRGIDRSEVERGQVLAKPKTIVPHTQFEATVYVLKKEEGGRHTPFFHNYKPQFYFRTTDVTGGIEFKPGREMVVPGDNVELTVTLIAPIAIEEGTKFSIREGGRTVGAGSVTKILK", "aa_hexdigest": "094091eaca3165ab26beac6d706d2680", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000129DB6", "ncbi_nrp_id": "WP_012855329.1", "uniref100_id": "UniRef100_P22679", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB6", "RefSeq:WP_012855329.1", "UniRef:UniRef100_P22679" ] }, "ips": { "uniref100_id": "UniRef100_P22679", "uniref90_id": "UniRef90_P22679" }, "psc": { "uniref90_id": "UniRef90_P22679", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_Q8Y422", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q8Y422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8Y422" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "LMJBGEANGM_182", "locus": "GCA013368015_00142" }, { "type": "cds", "sequence": "contig_1", "start": 82339, "stop": 83721, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936645.1", "SO:0001217", "UniParc:UPI0015897410", "UniRef:UniRef100_UPI0015897410", "UniRef:UniRef50_A0A6A8Q130", "UniRef:UniRef90_UPI0015897410", "PFAM:PF04778.17" ], "nt": "ATGAATGAAAAAAAGAAAAAAATTGCCATTCCATTGGCAATTTTGTGCGGCGGTTTAGCAATTGCTACAACAGCGCTTATTGCAATAAAGGCTCGCCGTCATAAAATTGCTAATCAATTGCAAAAAGAAAATTTATTGCAAAATTTTAAAAAATTACAAAAGCAATTAAACGAATTGCTAGGATATAAAATTGTTAATGAAATAAATGTTTTTCATGAACAAGAAGTTTTGCAAGGATCACTAAAAATTAATAATAAATCTGAAACCAAAGTAATTGAAGAAGAAACTTTAAGGTTAAAAGATGCTATTACTTTATTAATATCAAAAATAAAAAATCAAATAAATCAAAAAGAATTGGAATTTGCTAAATTTAATGAAATAAAAGATAAATTGCAAGAATATATCAAAAACGAGTTATCGAAACAAGAATACGAACATATTAAACAAAATATCGAAAATGAATTAAATAAATATACTCCAATTAGTCTTGAATCAACTTTAATCGAAATACAAAATGCAACAAATAATCTAATTAAATTATTAAATGAATCAACCAAAGAAAAAGATAATATTGATAATTTAAATGCCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAAGCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCAAAATTACACAGCATTAG", "aa": "MNEKKKKIAIPLAILCGGLAIATTALIAIKARRHKIANQLQKENLLQNFKKLQKQLNELLGYKIVNEINVFHEQEVLQGSLKINNKSETKVIEEETLRLKDAITLLISKIKNQINQKELEFAKFNEIKDKLQEYIKNELSKQEYEHIKQNIENELNKYTPISLESTLIEIQNATNNLIKLLNESTKEKDNIDNLNAKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKASLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKEIKITQH", "aa_hexdigest": "9678676fe4093913a77341ec58378f07", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0015897410", "ncbi_nrp_id": "WP_175936645.1", "uniref100_id": "UniRef100_UPI0015897410", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015897410", "RefSeq:WP_175936645.1", "UniRef:UniRef100_UPI0015897410" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015897410", "uniref90_id": "UniRef90_UPI0015897410" }, "psc": { "uniref90_id": "UniRef90_UPI0015897410", "uniref50_id": "UniRef50_A0A6A8Q130" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q130", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q130" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 157, "aa_cov": 0.34130434782608693, "hmm_cov": 1.0, "evalue": 4.85768595951198e-98, "score": 321.0541076660156, "start": 206, "stop": 362 } ], "seq_stats": { "molecular_weight": 51837.05570000013, "isoelectric_point": 9.02352695465088 }, "id": "LMJBGEANGM_183", "locus": "GCA013368015_00143" }, { "type": "cds", "sequence": "contig_1", "start": 83826, "stop": 86096, "strand": "+", "frame": 3, "gene": null, "product": "DUF6161 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C1B", "UniRef:UniRef90_UPI001F519C1B" ], "nt": "GTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAACGCATTAGATTGTTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAG", "aa": "MAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNKSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIVKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKEIQITQRIRLFNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNKSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIVKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKEIQITQH", "aa_hexdigest": "5b3372c69d32542cca7c539ab38dfea0", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F519C1B", "query_cov": 1.0, "subject_cov": 0.9593908629441624, "identity": 0.9990000000000001, "score": 1056.0, "evalue": 0.0, "valid": true, "product": "Family of unknown function (DUF6161)", "uniref50_id": "UniRef50_UPI001F519C1B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F519C1B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C1B" ] }, "genes": [], "id": "LMJBGEANGM_184", "locus": "GCA013368015_00144" }, { "type": "cds", "sequence": "contig_1", "start": 86201, "stop": 87043, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F52B277", "UniRef:UniRef90_UPI001F52B277", "PFAM:PF04778.17" ], "nt": "GTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAG", "aa": "MAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIVKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKEIQITQH", "aa_hexdigest": "3676440ecf1cd23f8923fb345df94918", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F52B277", "query_cov": 1.0, "subject_cov": 0.8974358974358975, "identity": 0.996, "score": 389.0, "evalue": 1.68e-133, "valid": true, "uniref50_id": "UniRef50_UPI001F52B277" }, "pscc": { "uniref50_id": "UniRef50_UPI001F52B277", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F52B277" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 157, "aa_cov": 0.5607142857142857, "hmm_cov": 1.0, "evalue": 8.387765550094845e-98, "score": 320.28326416015625, "start": 26, "stop": 182 } ], "seq_stats": { "molecular_weight": 30870.91410000003, "isoelectric_point": 8.94713191986084 }, "id": "LMJBGEANGM_185", "locus": "GCA013368015_00145" }, { "type": "cds", "sequence": "contig_1", "start": 87148, "stop": 89412, "strand": "+", "frame": 1, "gene": null, "product": "DUF6161 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C1B", "UniRef:UniRef90_UPI001F519C1B" ], "nt": "GTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAG", "aa": "MAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNKSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIVKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNKSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIVKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKEIQITQH", "aa_hexdigest": "7c92dcdc59e9a32af9cc37087ebbe4b6", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F519C1B", "query_cov": 1.0026525198938991, "subject_cov": 0.9593908629441624, "identity": 0.98, "score": 1025.0, "evalue": 0.0, "valid": true, "product": "Family of unknown function (DUF6161)", "uniref50_id": "UniRef50_UPI001F519C1B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F519C1B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C1B" ] }, "genes": [], "id": "LMJBGEANGM_186", "locus": "GCA013368015_00146" }, { "type": "cds", "sequence": "contig_1", "start": 89517, "stop": 90113, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001389CD37", "UniRef:UniRef90_UPI001F51D67C", "PFAM:PF04778.17" ], "nt": "GTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACCGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGGTAAAAGCTAA", "aa": "MAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNRSDIIAANNELKQALEVAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNGKS", "aa_hexdigest": "24b395ed2612cda55080d6651d475aa9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F51D67C", "query_cov": 1.0, "subject_cov": 0.8608695652173913, "identity": 0.995, "score": 292.0, "evalue": 6.03e-98, "valid": true, "uniref50_id": "UniRef50_UPI001389CD37" }, "pscc": { "uniref50_id": "UniRef50_UPI001389CD37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001389CD37" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 157, "aa_cov": 0.7929292929292929, "hmm_cov": 1.0, "evalue": 2.399924003266283e-62, "score": 205.06610107421875, "start": 26, "stop": 182 } ], "seq_stats": { "molecular_weight": 21521.6669, "isoelectric_point": 9.376426887512206 }, "id": "LMJBGEANGM_187", "locus": "GCA013368015_00147" }, { "type": "cds", "sequence": "contig_1", "start": 90100, "stop": 90348, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936646.1", "SO:0001217", "UniParc:UPI00158C267A", "UniRef:UniRef100_UPI00158C267A", "UniRef:UniRef50_UPI00158C267A", "UniRef:UniRef90_UPI00158C267A", "PFAM:PF04778.17" ], "nt": "ATGGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAG", "aa": "MVKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKEIQITQH", "aa_hexdigest": "9f6d6afdbddd3f3d3226503e296388e8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158C267A", "ncbi_nrp_id": "WP_175936646.1", "uniref100_id": "UniRef100_UPI00158C267A", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C267A", "RefSeq:WP_175936646.1", "UniRef:UniRef100_UPI00158C267A" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C267A", "uniref90_id": "UniRef90_UPI00158C267A" }, "psc": { "uniref90_id": "UniRef90_UPI00158C267A", "uniref50_id": "UniRef50_UPI00158C267A" }, "pscc": { "uniref50_id": "UniRef50_UPI00158C267A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158C267A" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 75, "aa_cov": 0.9146341463414634, "hmm_cov": 1.0, "evalue": 2.686992325475166e-31, "score": 104.17118835449219, "start": 2, "stop": 76 } ], "seq_stats": { "molecular_weight": 9326.323099999996, "isoelectric_point": 7.966374397277832 }, "id": "LMJBGEANGM_188", "locus": "GCA013368015_00148" }, { "type": "cds", "sequence": "contig_1", "start": 90453, "stop": 91037, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001389CD37", "PFAM:PF04778.17" ], "nt": "GTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTACCGCAAATCAAGCATTACAACAAGCATTAAATACCGCAAAGGCTAAAAAATCAAGTATAGATAATGAATTAAGGCCTCTAAAGAATGATTTGCAAAGTAAAATTGATGAGTTTGGACCTATAAGAAATACTAATTTTTCATGA", "aa": "MAKAAKVNADSTNNSIKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEVKNANQAVTSNNTASMQSAKSSLDTKINQINQQLQQFNKDKDAKFKELEQTRKDIDSFLTEDVKRNPNYTALVNTLTSAKTAKQSINKSSNKSDIITANQALQQALNTAKAKKSSIDNELRPLKNDLQSKIDEFGPIRNTNFS", "aa_hexdigest": "5f5969040e61e42f6556317c900e9138", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F51D67C", "query_cov": 0.9690721649484536, "subject_cov": 0.8173913043478261, "identity": 0.846, "score": 226.0, "evalue": 5.05e-72, "valid": false, "uniref50_id": "UniRef50_UPI001389CD37" }, "pscc": { "uniref50_id": "UniRef50_UPI001389CD37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001389CD37" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 158, "aa_cov": 0.8144329896907216, "hmm_cov": 1.0, "evalue": 3.2754184783889636e-64, "score": 211.12625122070312, "start": 26, "stop": 183 } ], "seq_stats": { "molecular_weight": 21358.536600000003, "isoelectric_point": 9.476352882385257 }, "id": "LMJBGEANGM_189", "locus": "GCA013368015_00149" }, { "type": "cds", "sequence": "contig_1", "start": 91179, "stop": 91307, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGACTATAACCGAAGAATTAGGAAAGGTTGAAACTAAAAATAGTAACATCGGATATAGATTGTTTAAATTGGCACAAGCTGAACAATTTAATAACACTGATGTTGATAAATTAAAGAACGCTTGA", "aa": "MKTITEELGKVETKNSNIGYRLFKLAQAEQFNNTDVDKLKNA", "aa_hexdigest": "47721fa3d1184cb13fa794f1ac6a7e3d", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4801.390600000002, "isoelectric_point": 8.064946556091307 }, "id": "LMJBGEANGM_190", "locus": "GCA013368015_00150" }, { "type": "cds", "sequence": "contig_1", "start": 91392, "stop": 91553, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTCAAGAATCTACAATTAAAAAAGTTATAGTGGATATTCAAGCACATATAAGAAATAACCTTAACATACAGCATAGATCAGGAGCTCAAAAGCTTATTGACAATATGAAGACATTTTATGGAGATAAAGTTGGTATAGAAAGCCTACAAAAATGA", "aa": "MSTQESTIKKVIVDIQAHIRNNLNIQHRSGAQKLIDNMKTFYGDKVGIESLQK", "aa_hexdigest": "15a3b6d428ee18d8ad4098cb1ea5ad83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6040.8843, "isoelectric_point": 9.600583457946776 }, "id": "LMJBGEANGM_191", "locus": "GCA013368015_00151" }, { "type": "cds", "sequence": "contig_1", "start": 91563, "stop": 91670, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGATAGTGTATTAAATGTAGATGATACTTTGAAAGACGACTTTAACAAAGCACTTAGGGTATTAGTTGGAGATTACACTAAAAACCCTCCTGTTTCATCGTGA", "aa": "MDDSVLNVDDTLKDDFNKALRVLVGDYTKNPPVSS", "aa_hexdigest": "aa1229084065a8b952d2472a21a23335", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3882.2652000000007, "isoelectric_point": 4.219522285461426 }, "id": "LMJBGEANGM_192", "locus": "GCA013368015_00152" }, { "type": "cds", "sequence": "contig_1", "start": 91728, "stop": 92084, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAGAGAAAATGAAATTTTATTAGATAAAGCAAAAGACTTAGATAAAAGAGCAGAAAAAACAATTAAATTTGTTGATGAAAATATTAATAGCCTTGACCAAAGAGCAAAAAGATTAAAACAAGAAATATTAAATGCTAAAAATGACTTAAGTAACTTTACACTAAATCATCAAAAAAACCAATTTACTGCAAAAGAAATAAACCCTAAAATTTCTCTATTAGAAAATAAATTAAATGAAATAAATCAATATCTTCTTCCAATAATAAGAGAAAAAGCAAAATCAAAAATATCGGAAATTGAAAAAAATAAAAAAGAACTTGAAGATATTATAAGAAGCAATTTTTATCTTTGA", "aa": "MVRENEILLDKAKDLDKRAEKTIKFVDENINSLDQRAKRLKQEILNAKNDLSNFTLNHQKNQFTAKEINPKISLLENKLNEINQYLLPIIREKAKSKISEIEKNKKELEDIIRSNFYL", "aa_hexdigest": "fb0fd86297f6031a34505b7c586fdb1c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13980.932099999982, "isoelectric_point": 9.245942878723145 }, "id": "LMJBGEANGM_193", "locus": "GCA013368015_00153" }, { "type": "cds", "sequence": "contig_1", "start": 92280, "stop": 92513, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F674", "UniRef:UniRef90_UPI0034A4F674" ], "nt": "TTGTTAACAGTTCCTGAATTTATTAAAGTTGTGCAAACAACTAGAAGCAATAACTTGCGGTCACTGTCAGAACAATTTAAATTTGTATTGTTAGACATTAATAAGTATTTAAAAGAAGTAAAGAAAATACTAGACGAAAATAAAACATTAAATAAGGATAACATAACTAAATTAAACGAAAAAATAAGATTGTTTGTGGACAAATCCCGTGAATTAAAAGAATCATTAAGATGA", "aa": "MLTVPEFIKVVQTTRSNNLRSLSEQFKFVLLDINKYLKEVKKILDENKTLNKDNITKLNEKIRLFVDKSRELKESLR", "aa_hexdigest": "5db99684ce7aa41ba9854ac7c8b255a6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 92301, "stop": 92597, "observations": { "mutation": [ 92511 ], "stop": [ 92511 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A4F674", "query_cov": 0.9090909090909091, "subject_cov": 0.7142857142857143, "identity": 0.8, "score": 102.0, "evalue": 7.68e-27, "gene_start": 8, "gene_end": 77, "reference_start": 1, "reference_end": 70, "reference_sequence": "MKVAQTTRSNNLRSLSEQFRITLLNIDKDLKEVKKILDENKTLDENNITKLNEKIKLFVDRSCELKESLRWFDNFHENISDQRIKNKLDEVRELLKNV" }, "paralog": false, "identity": 0.7959183673469388, "score": 150.0, "evalue": 2.36e-48, "description": "Internal stop codon at 92511. Nonsense mutation around 92511.", "psc": { "uniref90_id": "UniRef90_UPI0034A4F674", "uniref50_id": "UniRef50_UPI0034A4F674", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A4F674", "UniRef:UniRef50_UPI0034A4F674" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A4F674", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F674" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9195.686399999995, "isoelectric_point": 9.791796684265137 }, "id": "LMJBGEANGM_194", "locus": "GCA013368015_00154" }, { "type": "cds", "sequence": "contig_1", "start": 92810, "stop": 93736, "strand": "+", "frame": 2, "gene": null, "product": "Lmp protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C925", "UniRef:UniRef50_UPI001F51FD84", "UniRef:UniRef90_A0A454C925", "UniRef:UniRef90_UPI001F51FD84" ], "nt": "TTGACCTTTATTGCTGGGGGAGTTGCCATTACTGCTTTTGTTTTAATAATGTGTAGATTTTGCAAGAAACCTAATAAGCAAATAAAAGATCGAACCTCTTTAAATTTATTTAATAATGCGCAAAGTGAATTGAAAGAATTAATTAATTCTCCAATAGCCAAACTATGCCAAACCGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGCTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCTAAACAAGAATTAGAAAAATTCAACATGGCTATATTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTATAGATACCAAAAATTCAATCAATGCTTTAAATAATGCTAAAAAATTGCCTTTTAATTCTATTGAACAAGTTAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCAGAACCTTCTAAATATGTTGATAGTTCTAGTGAATTACAAACATTTAATGATTCAAATGTTGAAGCAAAATCAACACTTGAAGATATTGATAAAAAGATTGATGCCGCAATAAGAGCAAACAATGATTTAAATGAAAAAATTCAAAATTATAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAGATAAAATTAATGAAAAGATTAAAGAAGTAAAGGGCGTTGGAAAAAATTCGACAATCAAAGAATTACAAGTAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAAGCAATTAA", "aa": "MTFIAGGVAITAFVLIMCRFCKKPNKQIKDRTSLNLFNNAQSELKELINSPIAKLCQTAIEQKVLNDSNKINEKSTDEEISKNTEYLNSSIALLKNKLDLAKQELEKFNMAIFQLRSLVNNKDSKSIDTKNSINALNNAKKLPFNSIEQVKSESLTLKNNIDELKLKLKEVKKSRLAEAKNKLKAIIDSEPSKYVDSSSELQTFNDSNVEAKSTLEDIDKKIDAAIRANNDLNEKIQNYKSARLSDLESSRNDLSNFIANDLKDPVYKEIADKINEKIKEVKGVGKNSTIKELQVAIKTLADAKEKSN", "aa_hexdigest": "6c062462d162526aea9b31db260aa513", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI001F51FD84", "query_cov": 0.9967532467532467, "subject_cov": 0.8143236074270557, "identity": 0.941, "score": 516.0, "evalue": 1.56e-182, "valid": true, "uniref50_id": "UniRef50_UPI001F51FD84" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51FD84", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51FD84" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 92777, "stop": 94037, "observations": { "insertion": [ 93725 ], "mutation": [ 92807 ], "stop": [ 92807, 93984 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C925", "query_cov": 0.9967532467532467, "subject_cov": 0.48575949367088606, "identity": 0.98, "score": 538.0, "evalue": 4.49e-187, "gene_start": 1, "gene_end": 307, "reference_start": 12, "reference_end": 318, "reference_sequence": "MIKKRRRKLGWLTFIAGGVAITAFVLIMCRFCKKPNKQIKDRTSLNLFNNAQSELKELINSPIAKLCQTAIEQKVLNDSNKINEKSTDEEISKNTEYLNSSIALLKNKLDLAKQELEKFNMAMLQLRSLVNNKDSKSIDTKNSINALNNAKKLPFNSIEQVKSESLTLKNNIDELKLKLKEVKKSRLAEAKNKLKAIIDSEPSKYVDSSRELQIFNDSNVEAKSTLEDIDKKIDAAIRANNDLNEKIQNYKSARLSDLESSRNDLSNFIANDLKDPVYKEIADKINEKIKEVKGVGKNSTIKELQVAIKTLADAKEKAIKEKAWIDRVGINKVREIQRSKILLVDAEYIARFLRSGNGEPFRQKNEFNEYSGTEFFKLKNELERVSNLKDLNSLDSVDYKVIWKANEELKKFIPIINKAIKSYLYEIFEGWSDVLSISKLNVWDKYCYSPKYTGASEKLAAFKKEIQQKNEELRAKALDAKWMDIWVTYYPQLLKLLTVDIRDKFREVYITEEAKKNNLAYQVVDLLNKLESFDLNNSKLDKQEIKDFKEKLSAKNLEIEKYLSDKKNFESYRIYNPDLWMNNLSYDIKEQEKILNDINSKISLIEESSFTNQELKNTFAELKQKLSILNKN" }, "paralog": false, "identity": 0.9451073985680191, "score": 747.0, "evalue": 5.94e-272, "description": "Internal stop codon at 92807, 93984. Frameshift due to insertion around 93725. Nonsense mutation around 92807.", "psc": { "uniref90_id": "UniRef90_A0A454C925", "product": "Lmp protein", "uniref50_id": "UniRef50_A0A454C925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C925", "UniRef:UniRef50_A0A454C925" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C925", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C925" ], "product": "Lmp protein" } }, "id": "LMJBGEANGM_195", "locus": "GCA013368015_00155" }, { "type": "cds", "sequence": "contig_1", "start": 93714, "stop": 93986, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGAAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAATAAAGTTAGAGAAATTAGAAAATCTAGAATATTATTAGTTGATGCTGAATATATTGCAAGATTTTTAAAATCACAAAATGGTGAACGTTTTAGACAAGAAAATGAATTAGGCGAATTTCTCGGAACAAAATTTTTAAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAGGATTTAAATTCTCTTGATAGTGTTGATTATAAAGTAATATGA", "aa": "MLKKKAIKEKAWIDRVGINKVREIRKSRILLVDAEYIARFLKSQNGERFRQENELGEFLGTKFLKLKNELERVSNLKDLNSLDSVDYKVI", "aa_hexdigest": "0ad27b69d1f8ecd04456394cdd7c6396", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10638.312899999988, "isoelectric_point": 9.81777744293213 }, "id": "LMJBGEANGM_196", "locus": "GCA013368015_00156" }, { "type": "cds", "sequence": "contig_1", "start": 94380, "stop": 94517, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D", "UniRef:UniRef90_UPI001F1864BA" ], "nt": "TTGAACAATAGCAAATTAAATAAACAAGAAATTAAGAATTTTAAAGAAAAATTAATTGCAAAAAATCTAGAAATTGAAGAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGTATATATAACCCTAGTCTATGA", "aa": "MNNSKLNKQEIKNFKEKLIAKNLEIEEYLSSEETFKDYRIYNPSL", "aa_hexdigest": "9b1f78f0f94744b38287475023e804e2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 94380, "stop": 94676, "observations": { "mutation": [ 94515, 94533 ], "stop": [ 94515, 94533 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F1864BA", "query_cov": 1.0, "subject_cov": 0.45918367346938777, "identity": 0.867, "score": 75.1, "evalue": 1.29e-16, "gene_start": 1, "gene_end": 45, "reference_start": 1, "reference_end": 45, "reference_sequence": "MNNSKLDKQEIKDFKEKLSAKNLEIEKYLSSEETFKDYRAYNPDLWMNNLSYDIKEQEKILNDINSKISLIEENSFTNQELKNTFAELKQKLSILNKN" }, "paralog": false, "identity": 0.8061224489795918, "score": 152.0, "evalue": 7.81e-50, "description": "Internal stop codon at 94515, 94533. Nonsense mutation around 94515, 94533.", "psc": { "uniref90_id": "UniRef90_UPI001F1864BA", "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F1864BA", "UniRef:UniRef50_UPI002735871D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5454.1251, "isoelectric_point": 6.188140678405762 }, "id": "LMJBGEANGM_197", "locus": "GCA013368015_00157" }, { "type": "cds", "sequence": "contig_1", "start": 94756, "stop": 94932, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936647.1", "SO:0001217", "UniParc:UPI00158922EE", "UniRef:UniRef100_UPI00158922EE", "UniRef:UniRef50_UPI001F24906B", "UniRef:UniRef90_UPI001F24906B" ], "nt": "ATGAAACAAATTGAGAAAATATCTACTATAATTGAAAATTTTATAAAGTTAAAAAAGACAATGAAGATAACACTGTTCCTATTAAAATTTCCTATGTTTTATGAAAAAGAAATTTTTGCATCTCAATATATCCGCGAATTAGATGATATTTTAAACGAACTAAAAGATGCTCAATAA", "aa": "MKQIEKISTIIENFIKLKKTMKITLFLLKFPMFYEKEIFASQYIRELDDILNELKDAQ", "aa_hexdigest": "0c7b72493e473615e6e6b5359d7dad35", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158922EE", "ncbi_nrp_id": "WP_175936647.1", "uniref100_id": "UniRef100_UPI00158922EE", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158922EE", "RefSeq:WP_175936647.1", "UniRef:UniRef100_UPI00158922EE" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158922EE", "uniref90_id": "UniRef90_UPI001F24906B" }, "psc": { "uniref90_id": "UniRef90_UPI001F24906B", "uniref50_id": "UniRef50_UPI001F24906B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F24906B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F24906B" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7043.352999999999, "isoelectric_point": 7.963602256774902 }, "id": "LMJBGEANGM_198", "locus": "GCA013368015_00158" }, { "type": "cds", "sequence": "contig_1", "start": 95006, "stop": 95593, "strand": "-", "frame": 2, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "RefSeq:WP_012855332.1", "SO:0001217", "UniParc:UPI0001BC2078", "UniRef:UniRef100_D1J7J2", "UniRef:UniRef50_Q5ZZT0", "UniRef:UniRef90_D1J7J2" ], "nt": "ATGTATAAAACATTTAATGATGGAATGATAGAAGTTATAACTGGACCAATGTTTAGTGGAAAAAGTGAAGAATTATTAAGGAGAGTTAGAACTTTAGAATATGCTAAGATGCTACCGCTTTTAATAAAGCCCGAATATGATAATCGCTTTTCTGATGATGAAATAGTAAGCCGTTGTGGTGTAAGAAGAAAAACACATTCATTAAAAAACATTAACGAAGTTTATGAATTACTTAAACAAGATAAATATAAGGCAATAATAATTGACGAAGCCCATTTTTTTGGAAATTCATTAATTGAAGTCGCTGATGACTTAGCAAATAAGGGATATTTAGTAATTGTAGCTGGCCTAGACCAAGATTATCTAAGAAAACCCTTTGGGCCAATTCCTGCCTTAATGGCAATGGCTGAACGAGTAACTAAATTGCAAGCAATTTGTGTAGTATGTCAACATGCTGCTTCAACAAGTTTTAGAAAAGATTCTTCAAATGAGCAAAACTTAATTGGTGACTATAATGAATATGAAGCAAGATGCAGAAAGTGCCACATAATGGGTCAAAAACAAAAGGAATTACTAAAAAATAAGTAA", "aa": "MYKTFNDGMIEVITGPMFSGKSEELLRRVRTLEYAKMLPLLIKPEYDNRFSDDEIVSRCGVRRKTHSLKNINEVYELLKQDKYKAIIIDEAHFFGNSLIEVADDLANKGYLVIVAGLDQDYLRKPFGPIPALMAMAERVTKLQAICVVCQHAASTSFRKDSSNEQNLIGDYNEYEARCRKCHIMGQKQKELLKNK", "aa_hexdigest": "3f302cbda5058def0254b6f728fb0f1e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC2078", "ncbi_nrp_id": "WP_012855332.1", "uniref100_id": "UniRef100_D1J7J2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2078", "RefSeq:WP_012855332.1", "UniRef:UniRef100_D1J7J2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7J2", "uniref90_id": "UniRef90_D1J7J2" }, "psc": { "uniref90_id": "UniRef90_D1J7J2", "gene": "Tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_Q5ZZT0", "cog_id": "COG1435", "cog_category": "F", "go_ids": [ "GO:0004797", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZT0" ], "product": "Thymidine kinase" }, "genes": [ "tdk" ], "id": "LMJBGEANGM_199", "locus": "GCA013368015_00159" }, { "type": "cds", "sequence": "contig_1", "start": 95668, "stop": 96558, "strand": "+", "frame": 1, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_D1J7J3" ], "nt": "ATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACGTTATTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAGCCTAACGTTGCAATAGTTAATTTAGAAGATCCAAGACTTTGTGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCCGGATTAGTTGCTGGCGCTTCAAAAGGAGAAGGCTTAGGTAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAATAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATTAATAGAATTGGCAAAAAAGCTCAAAATTCAGGTGATAAAGAATTGAAATTCGAATTTGAAGTTGCCTTAAAAATACGTAATGCATTAGAAAATGAAATTATGGTTAAAAACATAGATTTGAGTCCAGAAGAACAAGTCGTGATCAAATCATATCAATTGCTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGTGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTATGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCGGGTAAAATCGAAGCAAGGGCATGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTLNEAEMANYAFTTIEPNVAIVNLEDPRLCELAKIVQTEKIVPATFQFVDIAGLVAGASKGEGLGNKFLANIREVDAIVHVIRCFENADIMHVNNKIDPISDLKTINIELILADLQTIENVINRIGKKAQNSGDKELKFEFEVALKIRNALENEIMVKNIDLSPEEQVVIKSYQLLTAKPTIYVANLDSDSFKNLESAKYYQELNKYLQDKKEILIPICIKLEYELCKFNDEEKEMFLNEYNIECSGLNLIIKKSFYLLNQATYFTAGKIEARA", "aa_hexdigest": "b49e3d8d12747a56af5bdd384ec3944a", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J7J3", "query_cov": 1.0, "subject_cov": 0.8065395095367848, "identity": 0.986, "score": 560.0, "evalue": 2.95e-200, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "LMJBGEANGM_200", "locus": "GCA013368015_00160" }, { "type": "cds", "sequence": "contig_1", "start": 96574, "stop": 96771, "strand": "+", "frame": 1, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "nt": "ATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTTGTAAAAGCAGAAGTCATTAAATATGAAGATTATGTTGCTTTAAATGGCGAAAAAGGTTGCAGGGAAGCCGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAA", "aa": "MNAAQCAGIIHTDFEKKFVKAEVIKYEDYVALNGEKGCREAGKIAIEGKNYIMQDGDVCFFRVAK", "aa_hexdigest": "204250049ecabfea5f2f80e5dd4fa1f8", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A380DK80", "query_cov": 0.9538461538461539, "subject_cov": 0.8051948051948052, "identity": 0.629, "score": 89.7, "evalue": 2.79e-22, "valid": false, "product": "GTP-binding and nucleic acid-binding protein YchF", "uniref50_id": "UniRef50_A0A382QXD8" }, "pscc": { "uniref50_id": "UniRef50_A0A382QXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "LMJBGEANGM_201", "locus": "GCA013368015_00161" }, { "type": "cds", "sequence": "contig_1", "start": 97100, "stop": 97315, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2CF1DD", "UniRef:UniRef90_UPI001F2CF1DD" ], "nt": "ATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCTCAAGAATCTGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAATTGCTAGTTTTTCTGATCATGATAATTTTTATATTGAAAGTTATTTGAAACGCCTTGAAATAATCAAACAATACAATATTGATATTACATTATGA", "aa": "MNLIKIDLHVHSQDSSHTGSNISQESDLEKLLILTKKRVKIASFSDHDNFYIESYLKRLEIIKQYNIDITL", "aa_hexdigest": "5b562b4ac7e1f1b4d03fc47c42168b77", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 97100, "stop": 97381, "observations": { "mutation": [ 97313 ], "stop": [ 97313 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2CF1DD", "query_cov": 1.0, "subject_cov": 0.7473684210526316, "identity": 1.0, "score": 139.0, "evalue": 1.81e-41, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNLIKIDLHVHSQDSSHTGSNISQESDLEKLLILTKKRVKIASFSDHDNFYIESYLKRLEIIKQYNIDITLWPGIEVNLRKYDGQIGQAIYIWSR" }, "paralog": false, "identity": 0.967741935483871, "score": 178.0, "evalue": 1.22e-59, "description": "Internal stop codon at 97313. Nonsense mutation around 97313.", "psc": { "uniref90_id": "UniRef90_UPI001F2CF1DD", "uniref50_id": "UniRef50_UPI001F2CF1DD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2CF1DD", "UniRef:UniRef50_UPI001F2CF1DD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2CF1DD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2CF1DD" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8302.384599999994, "isoelectric_point": 6.387872886657715 }, "id": "LMJBGEANGM_202", "locus": "GCA013368015_00162" }, { "type": "cds", "sequence": "contig_1", "start": 97499, "stop": 97669, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGGCAAGATATATGCAATAGCCAAGTTGATGGGCTGGATATAACAGATTTTAATAATAGCAACAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGA", "aa": "MVFPHAGKGQDNMEWQDICNSQVDGLDITDFNNSNKKKILKQDPNIPVLYFSDTHT", "aa_hexdigest": "b22cdf0bb33e5cc7f4ab21b8924eef2c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6409.0702, "isoelectric_point": 4.903579521179199 }, "id": "LMJBGEANGM_203", "locus": "GCA013368015_00163" }, { "type": "cds", "sequence": "contig_1", "start": 97804, "stop": 97971, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13476.12" ], "nt": "ATGATAAAAAGCCTAAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAGTCAAGTTAATGTTATTGTTGGTGAAAAAGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAACAAAAAATTATTCAAAAACGAATATGAATGA", "aa": "MIKSLKIKNNNETYDINLNSQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYE", "aa_hexdigest": "2fbc530a6b06a3a9185ba287e402b53f", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13476.12", "name": "AAA_23", "length": 28, "aa_cov": 0.509090909090909, "hmm_cov": 0.9285714285714286, "evalue": 6.5532680167052e-08, "score": 28.95298957824707, "start": 13, "stop": 40 } ], "seq_stats": { "molecular_weight": 6181.116799999998, "isoelectric_point": 9.050087928771973 }, "id": "LMJBGEANGM_204", "locus": "GCA013368015_00164" }, { "type": "cds", "sequence": "contig_1", "start": 98242, "stop": 98835, "strand": "+", "frame": 1, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C", "UniRef:UniRef90_UPI001E491A3C" ], "nt": "ATGTTAAAACAATTAGACGATTTTAAATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGACAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAGAGAAAAAATAAATAGTACAAATTTGGTTGTGAATAATCTTTTGTCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTGGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTCATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAAGATAAAAAAACTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTGAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGACAATATAATTCAATAATTGAAAAAGAAATCATTTTAAACTGA", "aa": "MLKQLDDFKLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETREKINSTNLVVNNLLSQFEQSSSLLQISNEQLLDIQQRTENVILQNNSFLEYYNQYNLVVSKDKKTLECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILN", "aa_hexdigest": "e24641f5b8300a1675f55bf484ff2fcd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 97942, "stop": 98862, "observations": { "mutation": [ 97969, 98833 ], "stop": [ 97969, 98833 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491A3C", "query_cov": 1.0, "subject_cov": 0.5549295774647888, "identity": 0.97, "score": 353.0, "evalue": 3.75e-120, "gene_start": 1, "gene_end": 197, "reference_start": 147, "reference_end": 343, "reference_sequence": "MIKSLKIKNNNETYDINLNSQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYEWFNEKAKFLVDSINIDNTEIKTQDFSYIFDSENKQNSKDAGILEIQKNLSGYISQNDSRKNSLDSSEYVEKQKSNCIDNFTEKIYYDQDQKMLKQLDDFRLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETKEKIYSTNLVVNNLLAQFEQSSSLLQISNEQLLDIQQRTENVILQNNLFLEYYNQYNLVVSKDKKALECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILNWTLEETDKKIRI" }, "paralog": false, "identity": 0.9673202614379085, "score": 575.0, "evalue": 4.73e-210, "description": "Internal stop codon at 97969, 98833. Nonsense mutation around 97969, 98833.", "psc": { "uniref90_id": "UniRef90_UPI001E491A3C", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491A3C", "UniRef:UniRef50_UPI001E491A3C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C" ], "product": "AAA family ATPase" } }, "id": "LMJBGEANGM_205", "locus": "GCA013368015_00165" }, { "type": "cds", "sequence": "contig_1", "start": 99013, "stop": 99483, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D", "UniRef:UniRef90_UPI001E54A28D" ], "nt": "ATGATTAAGACATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGATACATTGAACAATGTTCCTAATGAACAATACTTATTGCTAGACCAAATTGAAGATAATCTAGACAATAAAACAATATATGAAAGAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGGCAAACAAATTTTTATCGTTACACATAACCCTAATATTGGAACATTGATAAAAGGCAATATAATAACAACAGATATTTTTAATACTAATTTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACTAAAGGAGATAAATAA", "aa": "MIKTLIKDKIKIYAGDEEYKNLSMGQKTLFGITHAIDTLNNVPNEQYLLLDQIEDNLDNKTIYERIVPMLQEQIKKGKQIFIVTHNPNIGTLIKGNIITTDIFNTNLNKKFVVNEIIREDNEIDTPQSLYLEGSMKALYERLKVHEEKINKTKGDK", "aa_hexdigest": "e4b0239d07094b9124fa2f588bb2fd08", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E54A28D", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 0.987, "score": 302.0, "evalue": 1.9e-103, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI001E54A28D" }, "pscc": { "uniref50_id": "UniRef50_UPI001E54A28D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D" ], "product": "ATP-binding protein" }, "genes": [], "id": "LMJBGEANGM_206", "locus": "GCA013368015_00166" }, { "type": "cds", "sequence": "contig_1", "start": 99483, "stop": 99605, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6", "UniRef:UniRef90_D1J7J6" ], "nt": "ATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGA", "aa": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDA", "aa_hexdigest": "0b5c43c96b7a5c397768c5edef3831e1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 99483, "stop": 99779, "observations": { "mutation": [ 99603 ], "stop": [ 99603 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J6", "query_cov": 1.0, "subject_cov": 0.40816326530612246, "identity": 1.0, "score": 78.2, "evalue": 6.29e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDAWNKENINNFIIRAISISDEKLSEPQLTESAQEQLQKGNKQIEFIKNLFTNFVERYNEN" }, "paralog": false, "identity": 0.9897959183673469, "score": 190.0, "evalue": 1.36e-64, "description": "Internal stop codon at 99603. Nonsense mutation around 99603.", "psc": { "uniref90_id": "UniRef90_D1J7J6", "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J6", "UniRef:UniRef50_D1J7J6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4604.149700000001, "isoelectric_point": 4.663718986511231 }, "id": "LMJBGEANGM_207", "locus": "GCA013368015_00167" }, { "type": "cds", "sequence": "contig_1", "start": 99886, "stop": 100410, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGAAGAATGGTAAAGAAGTTAAAATGTCAAAACGTTTAGGAACTTCTTTAACGTTAAGAGAACTAGCAGAAGAAGTTGGAAATGATGCAATTAGATACTTTCTAATTGATAGATCATATAATTCAAGAATTGACTTTGATATTAACAAAGTTACTGACAACAACGAAAATAACCCTATGTATATCATTAAATATGCTCATGCTCGTGCTTGCCAATTATTAGATAAAGTAGGAATTGAAAACCCTATTGCTTCTGATGAGTTGGATAATGAATATGCTCAAAAGCTTGTTGCAGAATTAAAAGAATACCCCGAATTAATTTCGACAATGGCCAAAACTTATAAAGTCAACTTGTTGCCTCCTTATCTATTAAAATTATCAAAGGCATTTAACTCATTTTATTCAAATACCAAAGTTTTAGGATCACCTAACGAACAAAGCCTTGCAGCCTTAGTAAAGGCGACAAAAATTGTTTTAGCTGATGGTTTAAAATTAATGGACATTGAAGCACCTGAAAAAATGTAA", "aa": "MKNGKEVKMSKRLGTSLTLRELAEEVGNDAIRYFLIDRSYNSRIDFDINKVTDNNENNPMYIIKYAHARACQLLDKVGIENPIASDELDNEYAQKLVAELKEYPELISTMAKTYKVNLLPPYLLKLSKAFNSFYSNTKVLGSPNEQSLAALVKATKIVLADGLKLMDIEAPEKM", "aa_hexdigest": "cd944fc5de318be4932856bb9ea673b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 117, "aa_cov": 0.6436781609195402, "hmm_cov": 1.0, "evalue": 1.688043839946006e-29, "score": 97.86124420166016, "start": 63, "stop": 174 } ], "seq_stats": { "molecular_weight": 19667.477199999998, "isoelectric_point": 6.618639183044434 }, "id": "LMJBGEANGM_208", "locus": "GCA013368015_00168" }, { "type": "cds", "sequence": "contig_1", "start": 100717, "stop": 101151, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGCTAACCCAACAGGATATTTGCATATTGGACATGCAAGAAATGCTGCTATTGGTGCAACATTGTGCAATATTCTAGAAAAAGCAGGTCACCGCGTTGTTCGAGAATATTTAGTTAATGATTATGGAAACCAAATGAATAAAATGGCAGATTCAATATTTGCTAGATACCAACAAATATTTAATCCTGAATTTCCTATGCCAGAAGATTCATACCATGGCGGAGATATGATAGAATTCGCTCAAGCATTTTATAACATTCACAAAGATGAATACAAAAATGTAGAATATACTGATGAAATTAGAAAAATCTTTAGAAGTTTCGGAAGAGATTTTGCTTTAAAAAATATTATTGAAGATATGAAAAAATTTGGAGTAACATTCGATATTTTCACATCAGAAGCAGAACAATATGCCAAAAATAGAGTATGA", "aa": "MSANPTGYLHIGHARNAAIGATLCNILEKAGHRVVREYLVNDYGNQMNKMADSIFARYQQIFNPEFPMPEDSYHGGDMIEFAQAFYNIHKDEYKNVEYTDEIRKIFRSFGRDFALKNIIEDMKKFGVTFDIFTSEAEQYAKNRV", "aa_hexdigest": "c95f49b58da952cd593cf220f4fbb490", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16721.65919999999, "isoelectric_point": 5.938958549499512 }, "id": "LMJBGEANGM_209", "locus": "GCA013368015_00169" }, { "type": "cds", "sequence": "contig_1", "start": 101257, "stop": 101511, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGAAAACAACGAAAGACATTATTATTGCTTCATTAAATGAAGCACTTGAAAAATTAAATATTTCTAAGCCTATTGTACTTACTGAATCTAAAAATTACGGCGACTATTCAAGCAATATTGCCCTAACATTGCAAAAGGATTTAGGTAAGAAGGCAATAGATATTGCCCAAGAAATAGTAAGAAATATTGATTTAAATAAATTTAAAGAAATTAGTGAAATAAAGGTTGCCGAACCTGGTTTTATTAATTTTTGA", "aa": "MKTTKDIIIASLNEALEKLNISKPIVLTESKNYGDYSSNIALTLQKDLGKKAIDIAQEIVRNIDLNKFKEISEIKVAEPGFINF", "aa_hexdigest": "c4fb2651a79feec6cd8afa5b3577dae8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 81, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9753086419753086, "evalue": 9.149178201271578e-20, "score": 66.67340087890625, "start": 6, "stop": 84 } ], "seq_stats": { "molecular_weight": 9409.805399999996, "isoelectric_point": 6.232872962951659 }, "id": "LMJBGEANGM_210", "locus": "GCA013368015_00170" }, { "type": "cds", "sequence": "contig_1", "start": 101572, "stop": 102054, "strand": "+", "frame": 1, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGAAAAGATATTATTTATGTGGCCCAACTGTTTATAACTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTCACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAAACAGAAAAAGAAATTTCAAGTTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAGATTGTAAGAGTTACTGATTCACTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAATGGCAATGTTTATTTTGACGTTTTAAAATTTAAAGATGTGTATGGAAAAATAAGCAACCAGAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAACGCTCACCATATGATTTCGCTTTATGA", "aa": "MKRYYLCGPTVYNYPHIGNLRPIVSFDLMIRAQRQLGEDVYFLHNITDIDDKIIAKAKQENKTEKEISSFYEAYYLNLFKLFSIEMPNKIVRVTDSLNDMYEYIQSLIEKGAAYQVNGNVYFDVLKFKDVYGKISNQKLANLISDSSNEVDKRSPYDFAL", "aa_hexdigest": "53e44d9bdfe39edeeac9ba27ba052aca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.8, "subject_cov": 0.9624060150375939, "identity": 0.672, "score": 190.0, "evalue": 1.14e-59, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "LMJBGEANGM_211", "locus": "GCA013368015_00171" }, { "type": "cds", "sequence": "contig_1", "start": 102280, "stop": 102699, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF01406.26" ], "nt": "TTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAAAATTATTCGGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACTGATGAATTACTTGCATCACTTCAAAAACAAATTAATAATATTGAGGTACATTTTAATAAAAGTCAATTGGAAGATATTGAATATACGCTAAACAAACAAACAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCCTTTAAGCAACTTAATGAGCAATTAAAATCACCACTGAATTATGGTAATGCATTTTTTGTATTTAAGGTTTTAGGCTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGA", "aa": "MNYKNQKMSKSIGNIIYAEEFLKNYSADTYKLLMLSTSYSKPINITDELLASLQKQINNIEVHFNKSQLEDIEYTLNKQTIDSILKNVSNLDFSLAFKQLNEQLKSPLNYGNAFFVFKVLGFKLVENKVSNDDKELYRK", "aa_hexdigest": "0d42cdc77610b911aa9205aac3a4eaea", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 42, "aa_cov": 0.302158273381295, "hmm_cov": 1.0, "evalue": 1.3487862354351058e-07, "score": 26.17093276977539, "start": 4, "stop": 45 }, { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 51, "aa_cov": 0.3669064748201439, "hmm_cov": 1.0, "evalue": 3.0541320312021856e-10, "score": 35.42304992675781, "start": 2, "stop": 52 } ], "seq_stats": { "molecular_weight": 16178.314199999988, "isoelectric_point": 8.631172370910647 }, "id": "LMJBGEANGM_212", "locus": "GCA013368015_00172" }, { "type": "cds", "sequence": "contig_1", "start": 102777, "stop": 103244, "strand": "+", "frame": 3, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173", "SO:0001217", "UniRef:UniRef50_C4XFX7", "UniRef:UniRef90_D1J7J9" ], "nt": "ATGAAAAAAATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAAATTTACTTGCAAAAAGGACTTGTTAACAACAATTTAAAATTTAAAAAGGTTGTTTATTTAGATAGAAATAGTCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGTAGTATTTTTAAAGACGACTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCTCAAAATTTTGGTGCTATTATACGTAGTGCCAATGTGTTTGGCTTTAAACATATAATCATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGTGTTTTAAAAACTAGCTCAGGGGGTTTTAAAAACGTTAAAGTAATTAAAGTTAACAGCTTATTTGATGCAATTGAGGAATTAAAAAACAAAGGTTTTTGA", "aa": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDDSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGF", "aa_hexdigest": "d681f93a5e53e2b540405c4a0d5a18dc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmB" ], "truncated": "3-prime", "pseudogene": { "start": 102777, "stop": 103469, "observations": { "mutation": [ 103242 ], "stop": [ 103242 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J9", "query_cov": 1.0, "subject_cov": 0.6739130434782609, "identity": 0.9940000000000001, "score": 309.0, "evalue": 2.14e-105, "gene_start": 1, "gene_end": 155, "reference_start": 1, "reference_end": 155, "reference_sequence": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGFWIYASALNQQAKKLNDAKFNEKIAIIVGNEGSGVSKTLLKHSDEIIYIEQDKESVQSLNVSVATGILLYTVFNKK" }, "paralog": false, "identity": 0.991304347826087, "score": 446.0, "evalue": 6.74e-162, "description": "Internal stop codon at 103242. Nonsense mutation around 103242.", "psc": { "uniref90_id": "UniRef90_D1J7J9", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "uniref50_id": "UniRef50_C4XFX7", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0006396", "GO:0008173" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J9", "UniRef:UniRef50_C4XFX7", "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX7" ], "product": "RNA 2-O ribose methyltransferase substrate binding domain-containing protein" } }, "id": "LMJBGEANGM_213", "locus": "GCA013368015_00173" }, { "type": "cds", "sequence": "contig_1", "start": 103535, "stop": 104470, "strand": "-", "frame": 2, "gene": "arcC", "product": "Carbamate kinase", "db_xrefs": [ "COG:COG0549", "COG:E", "RefSeq:WP_175936652.1", "SO:0001217", "UniParc:UPI00158CC450", "UniRef:UniRef100_UPI00158CC450", "UniRef:UniRef50_P78030", "UniRef:UniRef90_D1J7K0" ], "nt": "ATGAGTAGAATTGTTATTGCATTAGGCGGAAACGCTTTAGGAAATAACCCCGAAGAACAAAAAGAACTTGTCAAAGTTCCTGCAGCCAAAATTGCTGAATTAGTAAAAGCAGGACACGAAGTTATTGTTGGACACGGAAACGGACCACAAGTTGGAATGATTTTCAATGGTTTCGTTAGTGCTCACTCAGTTAATCATAAGTCTCCTTTAGTTCCATTGCCTGAAGCAGGTGCAATGAGCCAAGGATACATTGGATATCATATGGTTAATGCTATTACAAATGCATTTAAAAACGAAGGATTAAAAAACAAGGAAGTTTTATACATTTTAACTCAAACTCTTGTAGATCCAAAAGACCCTGCATTCCAAAATCCTACAAAACCAATTGGCCCATTCTTTGCAACAGAAGCAGAAGCAGTTGCTGCAAACCCAAATAGCACAATTGTTGAAGATGCAGGTAGAGGCTACAGAAAAGTTGTAGCATCTCCAAAACCATTAAACTTTGTTGGTATTAATCAAATTAAAAAAGCTATTGAATCAGGTGCTACCGTTATCGTTGGTGGTGGTGGTGGAATTCCTACTGTTGAAGATGAAAATGGCTTTATTAAAGGTGTAGATGGTGTTATTGACAAAGATTTCGCTTTAGCAAAAATGGCTGCATTAGCAAAAGCTGATTACTTTATTGTTCTAACAGCAGTTGACTTTGTTAAAGTAAATTACAACAAGCCAGATCAAAAAGATCTAAAACACGCTACAAAAGCAGAACTTGAAAAATACATTGCTGAAAAACAATTTGCCCCAGGATCAATGTTACCAAAGGTACAAGCAGCTATTAAATTCGTTGAAGAAGGCGGAAAAGCAGCATTTATTGGTGATCTAAAAGATCTTGAAAACATTCTAGCAGAAAAAATCGGTACAAAAGTTACTTTAAATTAA", "aa": "MSRIVIALGGNALGNNPEEQKELVKVPAAKIAELVKAGHEVIVGHGNGPQVGMIFNGFVSAHSVNHKSPLVPLPEAGAMSQGYIGYHMVNAITNAFKNEGLKNKEVLYILTQTLVDPKDPAFQNPTKPIGPFFATEAEAVAANPNSTIVEDAGRGYRKVVASPKPLNFVGINQIKKAIESGATVIVGGGGGIPTVEDENGFIKGVDGVIDKDFALAKMAALAKADYFIVLTAVDFVKVNYNKPDQKDLKHATKAELEKYIAEKQFAPGSMLPKVQAAIKFVEEGGKAAFIGDLKDLENILAEKIGTKVTLN", "aa_hexdigest": "20cab915c37f714dd0297cff8bda8317", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158CC450", "ncbi_nrp_id": "WP_175936652.1", "uniref100_id": "UniRef100_UPI00158CC450", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CC450", "RefSeq:WP_175936652.1", "UniRef:UniRef100_UPI00158CC450" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CC450", "uniref90_id": "UniRef90_D1J7K0" }, "psc": { "uniref90_id": "UniRef90_D1J7K0", "gene": "ArcC", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "LMJBGEANGM_214", "locus": "GCA013368015_00174" }, { "type": "cds", "sequence": "contig_1", "start": 104472, "stop": 104720, "strand": "-", "frame": 1, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGGCAATGATCAAAGCAGCAAAAGAAAATGTTATCTTCCTACACTGCTTACCAGCATTCCACGATGACCAAACTCAATTCTCAGCAACTATTAAAGAAACCTTAGGTGCAAAATACCCAGTTGTTTCAACCGGTGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATACAACAAATCAATCGAACAAGCTGGAAACCGTATGCACACAATTAAGGCTGTTATTCTAGCTACTTTAGGATACTAA", "aa": "MAMIKAAKENVIFLHCLPAFHDDQTQFSATIKETLGAKYPVVSTGAMEVTDEVFQSKYNKSIEQAGNRMHTIKAVILATLGY", "aa_hexdigest": "0e8f710bbe858e2fe23fe497b4f89f6b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 75.1, "evalue": 4.39e-16, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "LMJBGEANGM_215", "locus": "GCA013368015_00175" }, { "type": "cds", "sequence": "contig_1", "start": 104934, "stop": 105095, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGCTAGCTGACTACTTAACATTTAAAGAACACGTTGGAGACCTAAGAGGAAAGAAAATTGTTTTCTCAGGTGATATTAAAAACAACGTTGCTCGTTCATTAATGATTGGTGCTGCATTCTTTGGTGTTAATATTGTATTATGTGGACCAAAAGCTCAATGA", "aa": "MLADYLTFKEHVGDLRGKKIVFSGDIKNNVARSLMIGAAFFGVNIVLCGPKAQ", "aa_hexdigest": "d35d8a671afba032d3529a3fb2aa2a80", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 36, "aa_cov": 0.6792452830188679, "hmm_cov": 1.0, "evalue": 4.490199759851032e-09, "score": 32.08402633666992, "start": 17, "stop": 52 } ], "seq_stats": { "molecular_weight": 5754.7484, "isoelectric_point": 9.428968620300292 }, "id": "LMJBGEANGM_216", "locus": "GCA013368015_00176" }, { "type": "cds", "sequence": "contig_1", "start": 105129, "stop": 105515, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAATTTAAAGGGTCGTAGTTTAGACAGCGCTCTAAACTTTACAACCGAAGAAATTAACTATTTACTAGATTTATCACTAGAACTAAAAAGAGCAAAATTTGCTGGTTTACACACAAATAATCGCCCATTAGTTGGAAAAAACATTGTTATAATGTTCCAAAAAGACTCAACAAGAACTCGTTGTTCATTTGAAGTTGCAGCTGCTGATTTAGGTGCAAGCTGTACATACATTGGCCCAACAGGATCAAACTTTGGTAAAAAAGAATCAGTTATTGACACAGCTAAAGTTTTAGGCCAAATTTATGATGGTATTGAATTCCGTGGATTCAAACAATCAGATGTTGATGCATTAGTAAAATACTCAGGAATTCCTGTATGA", "aa": "MPLNLKGRSLDSALNFTTEEINYLLDLSLELKRAKFAGLHTNNRPLVGKNIVIMFQKDSTRTRCSFEVAAADLGASCTYIGPTGSNFGKKESVIDTAKVLGQIYDGIEFRGFKQSDVDALVKYSGIPV", "aa_hexdigest": "06307b4f1814626b0733c2ae428eb8cc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.563, "score": 144.0, "evalue": 8.79e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 3.564376980838124e-30, "score": 100.5682373046875, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 14021.913599999985, "isoelectric_point": 8.562320137023928 }, "id": "LMJBGEANGM_217", "locus": "GCA013368015_00177" }, { "type": "cds", "sequence": "contig_1", "start": 105709, "stop": 105957, "strand": "-", "frame": 3, "gene": "mscL", "product": "Large-conductance mechanosensitive channel", "db_xrefs": [ "COG:COG1970", "COG:M", "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_D1J7K2", "UniRef:UniRef90_D1J7K2" ], "nt": "TTGGGTGCTTTAATTCAATTTATTATTGTTTCAACATTTATTTTTATAGGATTAATGATTGTATTTACAATTAAGAATTCTATTCTATATGCAAAAGCTAAAAAGAACCCAATTGAAGAAGAAATAAAGGAAGAACCTATTCCAACAACAGAAGAATTAATTTTAAATGAATTAAAAAAATTAAATGAAAATTTATTAGAAACGAAAAATTCTGGGAATAAAAAAGATTTAAAAAACGATTTAAAATAA", "aa": "MGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKNPIEEEIKEEPIPTTEELILNELKKLNENLLETKNSGNKKDLKNDLK", "aa_hexdigest": "2403515ac7873ccf41c2d062b2d5d31e", "start_type": "TTG", "rbs_motif": null, "genes": [ "mscL" ], "truncated": "3-prime", "pseudogene": { "start": 105709, "stop": 106254, "observations": { "mutation": [ 106011, 106020 ], "stop": [ 106011, 106020 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K2", "query_cov": 1.0, "subject_cov": 0.4293193717277487, "identity": 0.9390000000000001, "score": 135.0, "evalue": 1.18e-38, "gene_start": 1, "gene_end": 82, "reference_start": 110, "reference_end": 191, "reference_sequence": "FLLIKKTKTRIKFILYNIDMTSKELEEKKHYIKKSYVDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAIGMKNLDAWVVWPGIHATKETGGIFIGKFLGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKSPIEEEIKEEPTPTTEELILNELKKLNENLLETKNSGDKKDLKNNLK" }, "paralog": false, "identity": 0.9447513812154696, "score": 328.0, "evalue": 2.74e-117, "description": "Internal stop codon at 106011, 106020. Nonsense mutation around 106011, 106020.", "psc": { "uniref90_id": "UniRef90_D1J7K2", "gene": "MscL", "product": "Large-conductance mechanosensitive channel", "uniref50_id": "UniRef50_D1J7K2", "cog_id": "COG1970", "cog_category": "M", "kegg_orthology_id": "K03282", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K2", "UniRef:UniRef50_D1J7K2", "COG:COG1970", "COG:M", "KEGG:K03282" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K2" ], "product": "Large-conductance mechanosensitive channel" } }, "id": "LMJBGEANGM_218", "locus": "GCA013368015_00178" }, { "type": "cds", "sequence": "contig_1", "start": 106018, "stop": 106227, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01741.23" ], "nt": "ATGACAAGTAAAGAATTAGAAGAAAAAAAGCATTATATTAAAAAGTCATATATAGATGCAAAAAAAATAGTTTCGCGTGGAAACATGTTTATGCTAGCTATTGGTCTTTTATTAGGAGCTTCATTTGGGGCACTAGTATCATCACTAGCAAATGATGTTATTATGTCAGCAATCACAAAGGCGGTCGGTATGAAAAATCTTGATGCTTGA", "aa": "MTSKELEEKKHYIKKSYIDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAVGMKNLDA", "aa_hexdigest": "f66c04029516805ebdcad7631d08d727", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01741.23", "name": "MscL", "length": 47, "aa_cov": 0.6811594202898551, "hmm_cov": 1.0, "evalue": 6.758428017992955e-13, "score": 44.62319564819336, "start": 20, "stop": 66 } ], "seq_stats": { "molecular_weight": 7462.8359999999975, "isoelectric_point": 9.573893547058105 }, "id": "LMJBGEANGM_219", "locus": "GCA013368015_00179" }, { "type": "tRNA", "sequence": "contig_1", "start": 106299, "stop": 106391, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 68.9, "nt": "GGGTAAGTACTCAAGTGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGCTAAAACACCCGCGGAGGTTCAAATCCTCTCTTACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 106355, 106357 ], "id": "LMJBGEANGM_33", "locus": "GCA013368015_00180" }, { "type": "cds", "sequence": "contig_1", "start": 106459, "stop": 106626, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTATATTAGCATAGATCCTCAAAGCAATTATTATACAAAGAACATTGACTACTACAAGCAATTGCAAGAAGCTTCTAATCATGCTAAAAATTTAAAATTAGGAATTTTTGATAGTGGCAATGATATCAATAAAATTTACCCTCCAAGATCAAGGAAAAATTAA", "aa": "MYYISIDPQSNYYTKNIDYYKQLQEASNHAKNLKLGIFDSGNDINKIYPPRSRKN", "aa_hexdigest": "9df24ca2bbb1d30e9a19b08ce0ae9de5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6532.224200000001, "isoelectric_point": 9.260254859924316 }, "id": "LMJBGEANGM_220", "locus": "GCA013368015_00181" }, { "type": "cds", "sequence": "contig_1", "start": 106783, "stop": 107139, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTATCAAAAATAAAAAAACTTACTAAGATAGCTAAGCCAAATAATCAAAACGACACAAATAATGACAAATTTAACATCAATAATTTTCATGAAGCTAAATTTGATTTTGAAATACTAAAAAATAATAAATTTGAAGTAGGAAAAGCAACAGAAATAAATATTGCAAGGATTGCTGATGGAGATACAATATCAGATTCTTATCGAAACAAATATCGCTTTAATGGAATTGATACGCCAGAAACTCATAAAATGATTAATGGAAATTTCGAAGAAACATCCGGCATTCAATATAAATATGGAAAAATTGCCGAAAATTATACAAAGAATTTTCTAAACAATGCTGCTAAAATTTGA", "aa": "MLSKIKKLTKIAKPNNQNDTNNDKFNINNFHEAKFDFEILKNNKFEVGKATEINIARIADGDTISDSYRNKYRFNGIDTPETHKMINGNFEETSGIQYKYGKIAENYTKNFLNNAAKI", "aa_hexdigest": "bc1c2238da52b87c3619318c6877b76f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13615.09979999998, "isoelectric_point": 9.22221851348877 }, "id": "LMJBGEANGM_221", "locus": "GCA013368015_00182" }, { "type": "cds", "sequence": "contig_1", "start": 107158, "stop": 107412, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAAAAACTAAAAAAATATTGAATCTTGCATTGGGCTCTATTGCTACAATTTTGCCAATTTCAATAGTTTCATATTCTTGCAAACTAGAAAAAATTAATGACGAAGAACAACAAAATTTTAAAGAAAAATATGACAAAAAAATAAAGGAAATTGAAAATAGCAATGAATGGCAAAAAAATGATTATTATAAACAATTAATTGCTGATGCCAAAAAATTAATAAATTCAAATAGCAAAGAAGTTAATTGA", "aa": "MERKTKKILNLALGSIATILPISIVSYSCKLEKINDEEQQNFKEKYDKKIKEIENSNEWQKNDYYKQLIADAKKLINSNSKEVN", "aa_hexdigest": "ee0e54433da8d0696a1fb5f6e7eea48f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9851.184899999991, "isoelectric_point": 8.94597148895264 }, "id": "LMJBGEANGM_222", "locus": "GCA013368015_00183" }, { "type": "cds", "sequence": "contig_1", "start": 107699, "stop": 108088, "strand": "-", "frame": 2, "gene": "fba", "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "COG:COG0191", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7", "UniRef:UniRef90_D1J7K4" ], "nt": "ATGGGTGGCTATAAAACCGTTGTCAATTTGGTTAATGGTCTTATTCAAGATTTAGAAATTAAAACTAATGTCATATTGCACTTAGATCATGGTAATTATAACGATTGTATTAATGCTATAAATGCCGGCTTTAAGTCAATAATGTATGATGGTAGCAAAGAAGATTTTGAAACCAATATATTGAACACAAAAAAAATAGTTAACATTGCAAAAAAAATGGATATAACTGTTGAAGCAGAAGTAGGTAGAATTGGACTAGATATTAGTAAAAATAAATCACTTGATATTCAAAAAACAAACATTGAAGATGCTAAAAAATTAGCAAATACTGGAATTGATTTATTAGCAGTGGCAATTGGAAATATTCATGGTCAATATGAAAGTAATTGA", "aa": "MGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESN", "aa_hexdigest": "62dcf696b3b57efd0a16fdc9dc206a63", "start_type": "ATG", "rbs_motif": null, "genes": [ "fba" ], "truncated": "both", "pseudogene": { "start": 107396, "stop": 108280, "observations": { "mutation": [ 107701, 108169 ], "stop": [ 107701, 108169 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K4", "query_cov": 1.0, "subject_cov": 0.4387755102040816, "identity": 1.0, "score": 254.0, "evalue": 3.48e-83, "gene_start": 1, "gene_end": 129, "reference_start": 65, "reference_end": 193, "reference_sequence": "MIKLKRKNMEIQNLNQTLKKIKEENYAILHININNLEWAKFVLLATKQANLDIILGASPSAIKYMGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESNWKGLDFELLDKLNQEIKIPLVLHGASGIKEDDIKKAIRLGIKKINFNTELQIANAKALNDFLKNNNFLENKNYNPRILYANSNQAIYKCTLDLLNKYGKKN" }, "paralog": false, "identity": 0.9897959183673469, "score": 569.0, "evalue": 6.61e-210, "description": "Internal stop codon at 107701, 108169. Nonsense mutation around 107701, 108169.", "psc": { "uniref90_id": "UniRef90_D1J7K4", "gene": "Fba", "product": "Fructose-bisphosphate aldolase", "uniref50_id": "UniRef50_A0A3B0NXB7", "cog_id": "COG0191", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K4", "UniRef:UniRef50_A0A3B0NXB7", "COG:COG0191", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NXB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7" ], "product": "Fructose-bisphosphate aldolase" } }, "id": "LMJBGEANGM_223", "locus": "GCA013368015_00184" }, { "type": "cds", "sequence": "contig_1", "start": 108477, "stop": 108587, "strand": "-", "frame": 1, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003899", "GO:0006351", "SO:0001217", "UniRef:UniRef50_N9VBR1", "UniRef:UniRef90_D1J7K5" ], "nt": "ATGGAATATAAATCGCAAGTTGATATAGCCAAAGAAATTTTAGATAGACACGATGCACTAGATTTTGAAACTTTATTTAATTATGTAAAAGACGAACTACTTGAACGTTGA", "aa": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLER", "aa_hexdigest": "c8d54175b4f84f2b8947c9e2089e7878", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 108330, "stop": 108587, "observations": { "mutation": [ 108362, 108479 ], "stop": [ 108362, 108479 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K5", "query_cov": 1.0, "subject_cov": 0.4235294117647059, "identity": 1.0, "score": 72.8, "evalue": 5.06e-16, "gene_start": 1, "gene_end": 36, "reference_start": 1, "reference_end": 36, "reference_sequence": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLERWKEELTSSISDEELLIKKRGELYKLLTIDGRFFKKVNGLWTSKRPEFKN" }, "paralog": false, "identity": 0.9764705882352941, "score": 162.0, "evalue": 9.24e-54, "description": "Internal stop codon at 108362, 108479. Nonsense mutation around 108362, 108479.", "psc": { "uniref90_id": "UniRef90_D1J7K5", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_N9VBR1", "go_ids": [ "GO:0003899", "GO:0006351" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K5", "EC:2.7.7.6", "UniRef:UniRef50_N9VBR1", "GO:0003899", "GO:0006351" ] }, "pscc": { "uniref50_id": "UniRef50_N9VBR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VBR1" ], "product": "HTH HARE-type domain-containing protein" } }, "id": "LMJBGEANGM_224", "locus": "GCA013368015_00185" }, { "type": "cds", "sequence": "contig_1", "start": 109037, "stop": 109639, "strand": "+", "frame": 2, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "nt": "ATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTTTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGA", "aa": "MSVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTP", "aa_hexdigest": "a1b2024decd2b61298954f3300560145", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0005698E2F", "query_cov": 0.915, "subject_cov": 0.9104477611940298, "identity": 0.574, "score": 216.0, "evalue": 3.11e-68, "valid": false, "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI0005698E2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0005698E2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "product": "arginine deiminase family protein" }, "genes": [], "id": "LMJBGEANGM_225", "locus": "GCA013368015_00186" }, { "type": "cds", "sequence": "contig_1", "start": 109655, "stop": 109825, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATTAATGTTCCTAAATGA", "aa": "MKMSIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKDVEFKRIVAINVPK", "aa_hexdigest": "756abf8800f3830929735dd4d49184ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6252.2179000000015, "isoelectric_point": 6.125447273254394 }, "id": "LMJBGEANGM_226", "locus": "GCA013368015_00187" }, { "type": "cds", "sequence": "contig_1", "start": 109970, "stop": 110263, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTCGATGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTATGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTACCATTCCACGGAAACCAATTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGA", "aa": "MPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVK", "aa_hexdigest": "7c5eac652468c5e656c36d6582d9a4e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10354.017699999995, "isoelectric_point": 8.851718711853028 }, "id": "LMJBGEANGM_227", "locus": "GCA013368015_00188" }, { "type": "cds", "sequence": "contig_1", "start": 110385, "stop": 110681, "strand": "-", "frame": 1, "gene": "dut2", "product": "dUTP diphosphatase", "db_xrefs": [ "COG:COG4508", "COG:F", "SO:0001217", "UniRef:UniRef50_D1J7K7", "UniRef:UniRef90_D1J7K7" ], "nt": "ATGTTAGAAGAATATGCAGATGGAATTCATTTTATTACTAGCATTGCGCTAAAAAATAACATAACCTCATTTGATATAAAGCCAATAATTAAATATAATGACTTTACCTTGCAACTTGCTTATACATATCAATGCTTTACCAAATTGTTTTTGCACCATAATAAAAGATTAATAAAAAAAGCATACGGTGTTTATTTGGGACTAGGCGAAATTATGAATATTGACCGCGATATGATTTGCACGGCCTATGCCAAAAAAAATCATAAAAATTATGAAAGAATAAAAAATAATTATTAA", "aa": "MLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY", "aa_hexdigest": "9131402a92497820c79d2cde424a1510", "start_type": "ATG", "rbs_motif": null, "genes": [ "dut2" ], "truncated": "3-prime", "pseudogene": { "start": 110385, "stop": 110912, "observations": { "mutation": [ 110714 ], "stop": [ 110714 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K7", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 204.0, "evalue": 6.06e-66, "gene_start": 1, "gene_end": 98, "reference_start": 78, "reference_end": 175, "reference_sequence": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFASSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKYWKKDKSINQISMLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY" }, "paralog": false, "identity": 0.9885714285714285, "score": 344.0, "evalue": 1.65e-123, "description": "Internal stop codon at 110714. Nonsense mutation around 110714.", "psc": { "uniref90_id": "UniRef90_D1J7K7", "gene": "Dut2", "product": "dUTP diphosphatase", "uniref50_id": "UniRef50_D1J7K7", "cog_id": "COG4508", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K7", "UniRef:UniRef50_D1J7K7", "COG:COG4508", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K7" ], "product": "dUTP diphosphatase" } }, "id": "LMJBGEANGM_228", "locus": "GCA013368015_00189" }, { "type": "cds", "sequence": "contig_1", "start": 110712, "stop": 110912, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "ATGGAATTATTTTTAACTTTTAAGTTTATAATTCAAAACATGAATTTAACAGAAGTTTTTGAAGCCCAAAAGAAATTAGACCTTGCATTTGCAAATAGTATTGAAAAAACAGAATTTTTGAATATTAAAATCAAAATGGTTATTGCTTTACTTGTTGAACTTGGAGAATTTGCAAACGAAGTAAAACTATTTAAATATTGA", "aa": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFANSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKY", "aa_hexdigest": "616d1077d09d512230a2b21d98a99e41", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 52, "aa_cov": 0.7878787878787878, "hmm_cov": 1.0, "evalue": 3.2694491158960773e-10, "score": 36.042789459228516, "start": 15, "stop": 66 } ], "seq_stats": { "molecular_weight": 7762.191499999994, "isoelectric_point": 5.319015693664551 }, "id": "LMJBGEANGM_229", "locus": "GCA013368015_00190" }, { "type": "tRNA", "sequence": "contig_1", "start": 110947, "stop": 111030, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 69.5, "nt": "GCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 110980, 110982 ], "id": "LMJBGEANGM_3", "locus": "GCA013368015_00191" }, { "type": "cds", "sequence": "contig_1", "start": 111267, "stop": 112004, "strand": "+", "frame": 3, "gene": null, "product": "RDD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13", "UniRef:UniRef90_D1J7K8" ], "nt": "TTGCTAATATCTGCAATTGGTTTACTTGTTTTAATTTATATTACATTGGTCTTTAAAAACAAATATGAAGAATCTTTTGAACTTGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTAGGAATATTTTCAGGAGCATTATTAGTCTTGGCTTCTTTTATATTATTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAATAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAACTTAGTTAATGAAATTAATAGCGATTTTGCCAAACATTCATTTTTAGATCCAAATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGGTTAATTATCTTTGAAATTGTCATTCCTTTATTTATTTCTATTGCATTAATAGTATTAATTGCATGTCTTTTTAACAATGATGATCAACCAATCAGGGCAATTTATAGATTGATAATCATTTTAGTCAACGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTAAAAAATTATCAAAATTTCAATAATTTATATTTGCTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAG", "aa": "MLISAIGLLVLIYITLVFKNKYEESFELEIFNDIFVSCLVGIFSGALLVLASFILLDVFKRNKIKNFFEYYCYLSSLKSKNKFLFFKNYDLREIINNKNQLTKQEFICFVAEKLNYTMPSIEYKNLVNEINSDFAKHSFLDPNFKKQKKATLTRLIIFEIVIPLFISIALIVLIACLFNNDDQPIRAIYRLIIILVNAIIVLSLSILIYELFILSKVKNYQNFNNLYLLSFNNYSYKYLNSSLVR", "aa_hexdigest": "e90e711e42510a82fa49b0fa7eeba224", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7K8", "query_cov": 1.0, "subject_cov": 0.9074074074074074, "identity": 0.9840000000000001, "score": 443.0, "evalue": 2.29e-156, "valid": true, "uniref50_id": "UniRef50_N9TR13" }, "pscc": { "uniref50_id": "UniRef50_N9TR13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13" ], "product": "RDD domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_230", "locus": "GCA013368015_00192" }, { "type": "cds", "sequence": "contig_1", "start": 112367, "stop": 112588, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCCGCATCAGGACTTTCTGCTGCTTGTGGTCATACGGGTGGCGGTTCAGGAACTAGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCTTCAGGATTTTCTAACACAAATAAACAAGGTATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGA", "aa": "MKKINKILLGLAPAAALIAASGLSAACGHTGGGSGTSYGFDQTDDGKLVLASGFSNTNKQGIALKAVVDAYNK", "aa_hexdigest": "3afdec348b509f7875ec70c269bfb2cf", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7240.189900000001, "isoelectric_point": 9.25226078033447 }, "id": "LMJBGEANGM_231", "locus": "GCA013368015_00193" }, { "type": "cds", "sequence": "contig_1", "start": 112643, "stop": 112867, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATGGATATAATACCAATACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCCATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAATGGATTTAATTTGGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGA", "aa": "MANGYNTNTLRSDLDAKNSNDFYNMTLNYPAAASILSQYKMNLAISKEDYDKFGIENGFNLGNDLIGGNTKKEK", "aa_hexdigest": "38631733507daf8f03dc1e3ab2f14276", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8248.035399999997, "isoelectric_point": 5.125819969177246 }, "id": "LMJBGEANGM_232", "locus": "GCA013368015_00194" }, { "type": "cds", "sequence": "contig_1", "start": 112895, "stop": 113050, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGCTAAAGCATTAGTAGGAAAATTCATTAAAGAATTAAACGGCTTGGGTGTAAAAATTTCTGACACAGACAATACTAAAGTTAAGGAATATCTAGACTATTACACAACAAATAAAAATGAAGCACAAGGTGTTGATGAAACTTGA", "aa": "MLSVAKALVGKFIKELNGLGVKISDTDNTKVKEYLDYYTTNKNEAQGVDET", "aa_hexdigest": "1aff45f4de32d6629eee1020cae1fec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5653.327200000002, "isoelectric_point": 5.135198402404785 }, "id": "LMJBGEANGM_233", "locus": "GCA013368015_00195" }, { "type": "cds", "sequence": "contig_1", "start": 113114, "stop": 113425, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCAAATCTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACCAATGGAGATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGTGGATTTGACTACTCAACATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAAGATAGTTGACTTAATCCTAGAAGGTGTTAAAGAAGGCGCTGTTTGA", "aa": "MSDQIFRDYSSMINFSIQAKKMYNKDPLLNVIGFDSLPNVINTMTTSLTNGDINKGYIKPDEDKITTGGFDYSTFLKDANSEQSKLFKKIVDLILEGVKEGAV", "aa_hexdigest": "903d0011bfa8e4000d1f7d8253f2ee8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11537.007499999989, "isoelectric_point": 4.977186012268065 }, "id": "LMJBGEANGM_234", "locus": "GCA013368015_00196" }, { "type": "cds", "sequence": "contig_1", "start": 113480, "stop": 113626, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCTTGGATCAACTGCCGGATACTCACATACATTCTCAACTGCTAAATCATTAACAAACTTAAAAATAAAAGATACAACTACTTCATTAGATCAATCATCATTGGAAGTTTTAGGCCAAAAACCTGGTGAATCAGATAAATGA", "aa": "MSLGSTAGYSHTFSTAKSLTNLKIKDTTTSLDQSSLEVLGQKPGESDK", "aa_hexdigest": "3eeeff7617bcc3c7fcaa5c172eaf3da8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5061.543700000001, "isoelectric_point": 6.521330833435059 }, "id": "LMJBGEANGM_235", "locus": "GCA013368015_00197" }, { "type": "cds", "sequence": "contig_1", "start": 114089, "stop": 114478, "strand": "+", "frame": 2, "gene": null, "product": "Putative mycoplasma lipoprotein, C-terminal region", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230", "UniRef:UniRef90_UPI00215C1230" ], "nt": "ATGTTCCAACACGATTTAGAAAACTTTGAAATTGTTGAAGTTTCTAAAAAAGGAGAAACTAAAACAACATTTGACGGTAAGCAAAAACCTATTGATATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGGTAAAGCAAGCAAATTAAATAAAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTACATTCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCGTTATCAAAAGCAAACGTTAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAA", "aa": "MFQHDLENFEIVEVSKKGETKTTFDGKQKPIDIFNEYGSYISPTKSYFSSGKASKLNKATKIAFDNFKQLDNKDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL", "aa_hexdigest": "7e204d0ce4d4564c81ea7be4ff6fc2f4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 113924, "stop": 114478, "observations": { "mutation": [ 113963, 114086 ], "stop": [ 113963, 114086 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C1230", "query_cov": 1.0, "subject_cov": 0.6972972972972973, "identity": 0.907, "score": 222.0, "evalue": 2.98e-72, "gene_start": 1, "gene_end": 129, "reference_start": 57, "reference_end": 185, "reference_sequence": "MVSSNKILNEADADWISSPLAFDKNAKKAVFIQGPSILPIHANEKEDKATKLFINWMFQHDLENFKIEEVFKGKTTTKTFEGKQKPIDIFNEYGSYISPTKSYFSSDKASKLNKATKIAFDNFKQLDKEDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL" }, "paralog": false, "identity": 0.9130434782608695, "score": 321.0, "evalue": 9.74e-114, "description": "Internal stop codon at 113963, 114086. Nonsense mutation around 113963, 114086.", "psc": { "uniref90_id": "UniRef90_UPI00215C1230", "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C1230", "UniRef:UniRef50_UPI00215C1230" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230" ] } }, "id": "LMJBGEANGM_236", "locus": "GCA013368015_00198" }, { "type": "cds", "sequence": "contig_1", "start": 114566, "stop": 115105, "strand": "+", "frame": 2, "gene": "yncB", "product": "TNase-like domain-containing protein", "db_xrefs": [ "COG:COG1525", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7L0", "UniRef:UniRef90_D1J7L0" ], "nt": "ATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGCTCCGTTACTGTCCGTATCATGCAAGACAGTGGATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATGAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATGAAAAAGGAAATAAAACTATTAAAGAAGTTTCTCAATCATTATGGGATTATTATAATTATATTGAAGGCGAAGTTACTAGAATTTTTGATGGCGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAATGTTCCTACAACAATGAAATTAAGAATTCCAATGATTGATACATTAGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGA", "aa": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGNKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSN", "aa_hexdigest": "e00a36266a4667be429fe2602a8bff8a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 114566, "stop": 115408, "observations": { "mutation": [ 115103 ], "stop": [ 115103 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L0", "query_cov": 1.0, "subject_cov": 0.5559006211180124, "identity": 0.9940000000000001, "score": 348.0, "evalue": 3.96e-119, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGDKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSNWSEKTYDRFVGYVFFGKNFERQFDIEMLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNYWKKPKLATKKNNIYRYLEQINQNKK" }, "paralog": false, "identity": 0.9928571428571429, "score": 550.0, "evalue": 3.87e-201, "description": "Internal stop codon at 115103. Nonsense mutation around 115103.", "psc": { "uniref90_id": "UniRef90_D1J7L0", "gene": "YncB", "product": "TNase-like domain-containing protein", "uniref50_id": "UniRef50_D1J7L0", "cog_id": "COG1525", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L0", "UniRef:UniRef50_D1J7L0", "COG:COG1525", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L0" ], "product": "TNase-like domain-containing protein" } }, "id": "LMJBGEANGM_237", "locus": "GCA013368015_00199" }, { "type": "cds", "sequence": "contig_1", "start": 115181, "stop": 115459, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCAGGTGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCAGAATTTATTGCTGATTTATCAAAAGAAAGCACTGAAGCAAAGGAAATAAGAAGTTACTTGTTGCCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCATATGAACTAAGTTCAAAATATTTGGAACACGGACAAGGCATGGTTGATTTCTCACTTTCAATACTTCATTATAATTATTGA", "aa": "MLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNY", "aa_hexdigest": "8df4e3bfd313c4224aaa41d011cbb95d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10429.735599999998, "isoelectric_point": 6.0617876052856445 }, "id": "LMJBGEANGM_238", "locus": "GCA013368015_00200" }, { "type": "cds", "sequence": "contig_1", "start": 115546, "stop": 115944, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAGGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACAACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGGGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTCCAAAACTATGCACTATATCCTCACATGTCAGTTTACGAAAATATTGCATTTCCTCTATACAATGACAAACATTGA", "aa": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKH", "aa_hexdigest": "8d40a7fa5d1c53b48bb03bab9d1f50ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.5757575757575758, "hmm_cov": 1.0, "evalue": 2.746802690248361e-21, "score": 72.1390151977539, "start": 47, "stop": 122 } ], "seq_stats": { "molecular_weight": 14727.77869999998, "isoelectric_point": 8.66733913421631 }, "id": "LMJBGEANGM_239", "locus": "GCA013368015_00201" }, { "type": "cds", "sequence": "contig_1", "start": 115981, "stop": 116955, "strand": "+", "frame": 1, "gene": "potA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG3842", "COG:E", "SO:0001217", "UniRef:UniRef50_D1J7L1", "UniRef:UniRef90_D1J7L1" ], "nt": "TTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTTGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAGAAGTATTTAAATGAATTAATTTCACAATATAACGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACTTCGCTAACTAAAAATGTTTTGCAATATATTAAAAAACTAGGATTGCAACGCCAAGCAGAAATTTCAGAAGCTAGAGAAAAATTTTTGGGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTAAAAAATATATCAGTATTAAAATCAGAATTTTCAGATACTGTTTCTAAAATTAAATCTAAAAGTGCAGAAGAGATTGCTAATGTTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAACACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACGTGAACAAGCAGAATCTCCTTTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACTAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAATTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTGGATCAAGAAACCCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGACGAAGCAATCGTAAAAGATGTATTAGATGTAGCACAAAGAGTTGAAATTACAAAGAACTTAGCAAAACGTCCTACTCAAATGTCTGGTGGTCAACAACAACGTGTTGCCATAGCTAGAGCAATCGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGA", "aa": "MKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISVLKSEFSDTVSKIKSKSAEEIANVKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRK", "aa_hexdigest": "bee534d44b9c464a86dbbf479c2f2024", "start_type": "TTG", "rbs_motif": null, "genes": [ "potA" ], "truncated": "both", "pseudogene": { "start": 115681, "stop": 117258, "observations": { "mutation": [ 115942, 116953 ], "stop": [ 115942, 116953 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L1", "query_cov": 1.0, "subject_cov": 0.500772797527048, "identity": 0.997, "score": 515.0, "evalue": 1.2e-177, "gene_start": 1, "gene_end": 324, "reference_start": 146, "reference_end": 469, "reference_sequence": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKHWREETLEKTKLSQLKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISVLKSEFSDTVSKIKSKSAEEIANVKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9942857142857143, "score": 921.0, "evalue": 0.0, "description": "Internal stop codon at 115942, 116953. Nonsense mutation around 115942, 116953.", "psc": { "uniref90_id": "UniRef90_D1J7L1", "gene": "PotA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J7L1", "cog_id": "COG3842", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L1", "UniRef:UniRef50_D1J7L1", "COG:COG3842", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L1" ], "product": "ABC transporter ATP-binding protein" } }, "id": "LMJBGEANGM_240", "locus": "GCA013368015_00202" }, { "type": "cds", "sequence": "contig_1", "start": 117022, "stop": 117486, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6", "UniRef:UniRef90_UPI00215C93E5" ], "nt": "ATGTCAATTAGCGACAAGATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCTCAAATTAAAGACGGATATATAATTTACAACAATAAAAAACTACTTAAAGCTCCTGAAGGATATCAAAAAGCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAGGAAGAAGAAGGAATATTTGAAGGAAGCATAAAATTAGTTGAACATTTAGGTAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTACGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAA", "aa": "MSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKASIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV", "aa_hexdigest": "cc028970f521777878587e463934f672", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 116722, "stop": 117486, "observations": { "mutation": [ 116953 ], "stop": [ 116953 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C93E5", "query_cov": 1.0, "subject_cov": 0.42896935933147634, "identity": 0.9940000000000001, "score": 298.0, "evalue": 3.84e-99, "gene_start": 1, "gene_end": 154, "reference_start": 206, "reference_end": 359, "reference_sequence": "ANAKKEYSEKYNEALDRNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRCVLVSKITNVEYTEAVENVFGSLFNSQNITKGSFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9921259842519685, "score": 482.0, "evalue": 3.39e-174, "description": "Internal stop codon at 116953. Nonsense mutation around 116953.", "psc": { "uniref90_id": "UniRef90_UPI00215C93E5", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C93E5", "UniRef:UniRef50_A0A3G8LGF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "LMJBGEANGM_241", "locus": "GCA013368015_00203" }, { "type": "cds", "sequence": "contig_1", "start": 117585, "stop": 117860, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATACCAAGTTGGTGCGACGGTTTATGACAAGGGATTTGGATTAAAAAACTATATTAAGTTGTCAAAAGATCCAATATTCCACATTGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATCTCTATTTTGATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGA", "aa": "MLPSLITLTLFTIIPFILVLIFSSTYQVGATVYDKGFGLKNYIKLSKDPIFHIAIRNTLVYAILALPISLAISILISSAISFVIKQSFRRF", "aa_hexdigest": "960c2ff5d57665f278c0ba6e525ce04a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10181.19469999999, "isoelectric_point": 10.08996295928955 }, "id": "LMJBGEANGM_242", "locus": "GCA013368015_00204" }, { "type": "cds", "sequence": "contig_1", "start": 117764, "stop": 117973, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTACCCGCTAGCATGTTAATAAATCCGCCTGATGTTCTAAATAAGAATGCAAAGGCGATTGAAACAGCAATTCCTGATGTTACATATGGTAGAAAGAATACTGTTTGTCAAAATCTACGGAAACTTTGTTTGATTACAAAGCTTATTGCAGATGAAATCAAAATAGAGATCGCCAAGCTAATTGGTAAAGCTAATATAGCATAA", "aa": "MFLPASMLINPPDVLNKNAKAIETAIPDVTYGRKNTVCQNLRKLCLITKLIADEIKIEIAKLIGKANIA", "aa_hexdigest": "51dba5161a87c8001274008457b5bda5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7577.048199999994, "isoelectric_point": 9.342967796325684 }, "id": "LMJBGEANGM_243", "locus": "GCA013368015_00205" }, { "type": "cds", "sequence": "contig_1", "start": 118050, "stop": 118310, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCTTTCAAATTCTAATTTTAACAACCGCTATGCTATCGGTTAACCCTGATTTATATAAATCAGCTTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAAGCAGCTGCAAACTCTGGTATGAGTATCATGTTATACATATTCTGA", "aa": "MAFQILILTTAMLSVNPDLYKSASIDGSSNLKQFFAITLPSIRKTITFLFTVGIIGSIKTFPLALFDNKPAEAAANSGMSIMLYIF", "aa_hexdigest": "0d8d2ad53ba93c1e4304174b0070307b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9352.989999999993, "isoelectric_point": 9.22402362823486 }, "id": "LMJBGEANGM_244", "locus": "GCA013368015_00206" }, { "type": "cds", "sequence": "contig_1", "start": 118353, "stop": 118505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAA", "aa": "MILFLLGLLVSFGLRKLVSLVFFANKKIGEINVIRKIENKTLKRKVVFKI", "aa_hexdigest": "5b9267be914f908d8b08664ff4548470", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5789.191900000001, "isoelectric_point": 11.235179328918456 }, "id": "LMJBGEANGM_245", "locus": "GCA013368015_00207" }, { "type": "cds", "sequence": "contig_1", "start": 118627, "stop": 118863, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAATGTCATTTGTAGAAAAACAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGGGCAATAGTAGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGGTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCGATTACATTAGGATATGCACTATCATTAAGAAACTGA", "aa": "MIEMSFVEKQLSTDSRTTILFPRAIVEGGKSFFPENYRDAFESGFLEALVFTAGITTFSVLIRLGFSITLGYALSLRN", "aa_hexdigest": "41b59fdb68fc65d361c7dd353b33be8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8719.945599999992, "isoelectric_point": 5.221082115173339 }, "id": "LMJBGEANGM_246", "locus": "GCA013368015_00208" }, { "type": "cds", "sequence": "contig_1", "start": 118980, "stop": 119165, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTGTTCAGGTTGTTGCTGAAACCATTGGCATTGCTATTTTAAAGAAGTATTTGATTCCATTTGCGCCATCTAGCATTGATGATTCTTTAACACTGTTTGGGATTGCTTCAAATGCATTTTTATACATATATCCAAAGAATATTGAAGCAGCAAACGGCATAACTAATGCAAAGAATTGA", "aa": "MQTVQVVAETIGIAILKKYLIPFAPSSIDDSLTLFGIASNAFLYIYPKNIEAANGITNAKN", "aa_hexdigest": "fe5e88f4344ddbf725467d9010363c68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6573.5626, "isoelectric_point": 5.97044734954834 }, "id": "LMJBGEANGM_247", "locus": "GCA013368015_00209" }, { "type": "cds", "sequence": "contig_1", "start": 119387, "stop": 119611, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006401D33", "UniRef:UniRef100_A0A454C920", "UniRef:UniRef50_A0A454C920", "UniRef:UniRef90_A0A454C920" ], "nt": "ATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCGAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTATTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTATTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAA", "aa": "MQFQDKEMQQKDRIMEKDINVQLVKLSKYQKKSKIKKVFIYIGIGFVLISLIIIMILGVYGIIGGLDGLIKKGL", "aa_hexdigest": "8e8c01ea1f6a2a8601616c64e21b874f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0006401D33", "uniref100_id": "UniRef100_A0A454C920", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006401D33", "UniRef:UniRef100_A0A454C920" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C920", "uniref90_id": "UniRef90_A0A454C920" }, "psc": { "uniref90_id": "UniRef90_A0A454C920", "uniref50_id": "UniRef50_A0A454C920" }, "pscc": { "uniref50_id": "UniRef50_A0A454C920", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C920" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8472.313499999993, "isoelectric_point": 9.823321723937987 }, "id": "LMJBGEANGM_248", "locus": "GCA013368015_00210" }, { "type": "cds", "sequence": "contig_1", "start": 119716, "stop": 119871, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGA", "aa": "MAYGTKNLLIFKNNLIQNKQNLKSFALKLSFYVLKQMPQIKSVILAYDNEY", "aa_hexdigest": "35315d506ca26165cd48c6380d7df148", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5999.050600000001, "isoelectric_point": 9.757241630554198 }, "id": "LMJBGEANGM_249", "locus": "GCA013368015_00211" }, { "type": "cds", "sequence": "contig_1", "start": 119878, "stop": 121164, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4", "UniRef:UniRef90_D1J7L4" ], "nt": "ATGAGCCAATATGTAACTTATTATTTAAAATCCAGGGGTATAAATGTTTATTTTTTACATAAGAATCATTTAGGAGTTCCTTTTTCATTCTTGGAATTTGCTTTTAAAAAATTAAATACCTTGGATTTGGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAAATTCAAACTATTTTTAAAAATAATCAAGAGATAAATTTTTTTGATTTTAATAATTTCAATCTCAATGAAAATGAATATCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGTTGCTTAATGATTATTTAAGCCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAATAAATATCAATATAATTTTGAATTAATTAAGAAAATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAAATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTGTTAACAAGAGGCATGCAATTTAATTATTTTGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCGTATAAAAAATTATTTAATTATGATTTTAAGCAAATTTTAAATGAGCATTTAGTTGCATTATATATTAAATTTCAAAACACATTCTTTAACTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAATTTATATAAACCAACTTTATCCCACTCCTATTTTAATAAAGGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCAACAACAAGACATTGTTTTAAATGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGCGCTGAGTATAATTATAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAATTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAATTGAAATTAAAATTCTTAACTTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGACAAATTAAGTATTCAAAAATCAATATCTCGTATTGACATTGATCAATTAACAGTTTTCGAAAACAAACAAGACGCATATATAAAATATCTTGCTAAGATAACATTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCTTGCAAGTTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAGTATTTTAACAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAA", "aa": "MSQYVTYYLKSRGINVYFLHKNHLGVPFSFLEFAFKKLNTLDLAIYFSFFDSKSCKIQTIFKNNQEINFFDFNNFNLNENEYQFLNDEPIYLDTKMLLNDYLSQITSNRVTKYHNSILNLIINKYQYNFELIKKIMGFDDLGYKFINKQKFTNDTIFRVSKTLLTRGMQFNYFARFFINQEVKYYLAYKKLFNYDFKQILNEHLVALYIKFQNTFFNSMLINDNFFKINKIYINQLYPTPILIKVCEMFDIEYSFVLQQQDIVLNDNENVLYVDNNDISFLSAEYNYRDSFKTFFYINEMLNYFETQQIDIFDIEKELKLKFLTYKEFEIECSIANLVHFKDKLSIQKSISRIDIDQLTVFENKQDAYIKYLAKITLKTNPQCFISISYNEISCKLKFSVFYSQTTKIFNKIKEYFNKFLLLNKKIRN", "aa_hexdigest": "92d9937c0081105aad862966912f5846", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7L4", "query_cov": 1.0, "subject_cov": 0.8879668049792531, "identity": 0.963, "score": 815.0, "evalue": 1.22e-296, "valid": true, "uniref50_id": "UniRef50_D1J7L4" }, "pscc": { "uniref50_id": "UniRef50_D1J7L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 51835.13069999999, "isoelectric_point": 8.765137672424316 }, "id": "LMJBGEANGM_250", "locus": "GCA013368015_00212" }, { "type": "cds", "sequence": "contig_1", "start": 121180, "stop": 121572, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTGAATAATTTAGAGCTTTATAAAGTTGATAGTAAGGGTAAAAAGATAAATGTTCAAAGATTCCCTGATGGTACAAAAGCAATAGACGCTTTTAATATCTTTAGTGGTTATATTTCTCCTACAAATAAATATCTAAGCCAAGATGCAAAGAACGTAACAAAAAATATTGCCGAAGAAATAGCGTTCACAAACTTTAAACACTTAATCAAAGAAGGATCAATATACAAACCTGCCGATGATGTTGCAACTAGAAGAAGTGGTCAATTAAGAGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAATTTTTGACTACGATGAATTTATTAATAAAATTAAGCTTTTGTTCCAAGAATAA", "aa": "MYTQPLNNLELYKVDSKGKKINVQRFPDGTKAIDAFNIFSGYISPTNKYLSQDAKNVTKNIAEEIAFTNFKHLIKEGSIYKPADDVATRRSGQLRDAIKQAARSILSDANVLKIFDYDEFINKIKLLFQE", "aa_hexdigest": "0f4b46278d5feb545faf627407979496", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14838.748199999982, "isoelectric_point": 9.221573829650879 }, "id": "LMJBGEANGM_251", "locus": "GCA013368015_00213" }, { "type": "cds", "sequence": "contig_1", "start": 122071, "stop": 122223, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTACTTCAACAGCTGCATATCATTACACTTTTGTTAGTGAAAATGGAAAAAATAACAATTATACAATCAAGCAGGCAAACAGAGTGTTAAATCCAAACACAACAATTGAAATATTAAACGATACTCCCAAAAATAATGAAAAATGA", "aa": "MVITSTAAYHYTFVSENGKNNNYTIKQANRVLNPNTTIEILNDTPKNNEK", "aa_hexdigest": "e555693429336f0243fbba90845c7f61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5686.239400000001, "isoelectric_point": 8.085963249206543 }, "id": "LMJBGEANGM_252", "locus": "GCA013368015_00214" }, { "type": "cds", "sequence": "contig_1", "start": 122278, "stop": 122589, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTACAGCGATTTAATTAATTTTGCAATTCAAGCAAAAAGATTATACAACGCGGACCCTACATTAAACGTTTTGGGAATAGATGAAATACCTTCGGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGTCCAACAACTGGTGGCTACAATTATGACAATTTTATTGAAAAACCTGATAGCCCGCAAGCGAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAAGGGCATTCAAGAAGGTGCCGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNADPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCPTTGGYNYDNFIEKPDSPQAKLFKKVSSIILKGIQEGAV", "aa_hexdigest": "9234b174f3d6518ffd346b45e2cb6725", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11330.66199999999, "isoelectric_point": 4.520598411560059 }, "id": "LMJBGEANGM_253", "locus": "GCA013368015_00215" }, { "type": "cds", "sequence": "contig_1", "start": 122659, "stop": 122946, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAGCAATTGATGACAAAGATTTTAAAAAATATAACATTGAACCAGTATTTAATGTCGCAAACTCTTTAATTGCAAATAACAAATATAATAAAAAGTGGGCAATACCTCTATCTCGTTCAGGAGAAATGACTACAGTAGCTAAAGCATTAGTTGGAAAATTCGTTAAAGAATTAATGGCTCTAGGAGTTAAATTAGATAGTCAAAATAGTAGCAAATTGCAATCTTATTTAGACTATTACAATAATAACGATCAAGAAGCAAAAGGCGTTGATGGTATTTGA", "aa": "MNLAIDDKDFKKYNIEPVFNVANSLIANNKYNKKWAIPLSRSGEMTTVAKALVGKFVKELMALGVKLDSQNSSKLQSYLDYYNNNDQEAKGVDGI", "aa_hexdigest": "bfae701f8f75282c97b43b67bdfcb081", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10649.020299999995, "isoelectric_point": 8.802851676940918 }, "id": "LMJBGEANGM_254", "locus": "GCA013368015_00216" }, { "type": "cds", "sequence": "contig_1", "start": 123121, "stop": 123330, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGACTTGGCAAATTATTCACTTTAATGACTATAGGTTTACTTCCTACGACTACATTGATTTTATCCTCATGTTCAACAACAGAAAATTCGAAATACGGATTTGACCAAGAAGATGATGGCCAATTAGTATTATCAATGGGATTTTTAAAAAATGATTGGCAAGGGCTTGCTATTCAAGGGATTGTTGATACATACAACAAATGA", "aa": "MKRLGKLFTLMTIGLLPTTTLILSSCSTTENSKYGFDQEDDGQLVLSMGFLKNDWQGLAIQGIVDTYNK", "aa_hexdigest": "32ff6f0967a24eb9906f494b17a582b2", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7661.756600000001, "isoelectric_point": 4.9838361740112305 }, "id": "LMJBGEANGM_255", "locus": "GCA013368015_00217" }, { "type": "cds", "sequence": "contig_1", "start": 123590, "stop": 123982, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTGAATAATTTAGAACTTTATAAAGTTGATAGCAATGGCAAAAAAACAAACATTCAAAAATTCCCTGATGGTACAAAAGCAATAGATGCTTTCAACGCCTTTGGTGGATATATTTCTCCTACAAATAAATATCTAAGTCAAGATGCAAAGAGTGCAACAAAAAATATTGCTGATGAAATAGCATTCACAAACTTTAAACATTTAATCAATGAAGGATCGATATACAAACCTGCTGATGATATTGCAACTAGAAAAGGCGACCAATTAAGGGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAGTTTTTGACTACAAAGATTTTATTAATAAAATTAAGCTATTGTTCCAAGAATAA", "aa": "MYTQPLNNLELYKVDSNGKKTNIQKFPDGTKAIDAFNAFGGYISPTNKYLSQDAKSATKNIADEIAFTNFKHLINEGSIYKPADDIATRKGDQLRDAIKQAARSILSDANVLKVFDYKDFINKIKLLFQE", "aa_hexdigest": "14657af385a140e6505471275e2336d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14642.412599999976, "isoelectric_point": 9.001930046081544 }, "id": "LMJBGEANGM_256", "locus": "GCA013368015_00218" }, { "type": "cds", "sequence": "contig_1", "start": 124109, "stop": 124360, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAATTAATAAAGCTAAAAAATTACAAGGCAAGGCAGGCTATATAGTTTCTGGCTCTGAATATTCATACAATAAAGCTGAAGATAAAATTGAATTAAAAATAGGAAATAAAATAACTAAATTACAAGGAATTTATTTAGGCAAATTCTTTAATAATAATGAAAATGAATATTTCTTTATTAATCAAGAAGACGTGGATGTTAAAGTATTAAAGGCCAATAATTTTATTAACCAAGAAGATGCAGATTGA", "aa": "MSINKAKKLQGKAGYIVSGSEYSYNKAEDKIELKIGNKITKLQGIYLGKFFNNNENEYFFINQEDVDVKVLKANNFINQEDAD", "aa_hexdigest": "4137a8741f8e376b2cdcc20d01f18f16", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9500.577499999992, "isoelectric_point": 6.259246253967285 }, "id": "LMJBGEANGM_257", "locus": "GCA013368015_00219" }, { "type": "cds", "sequence": "contig_1", "start": 124481, "stop": 124639, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATAAATATTGGTTCTACTGCTGGATATCGTCACACATTCGTTGGCAATAATGAAAAAAATAGTACTTATATAATTAAACAAGCCAACAGGGTATTAGATCCAACAACAACAATTGAAATATTAAATAATACTCCTAAAAATAATGAAAAATGA", "aa": "MAINIGSTAGYRHTFVGNNEKNSTYIIKQANRVLDPTTTIEILNNTPKNNEK", "aa_hexdigest": "9f125fe2d5c33ffda7d748534f0afbda", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5806.436100000001, "isoelectric_point": 9.301836967468262 }, "id": "LMJBGEANGM_258", "locus": "GCA013368015_00220" }, { "type": "cds", "sequence": "contig_1", "start": 124688, "stop": 124999, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTAATTAACTTTGCAATTCAAGCAAAAAGATTATATAATGTTGACCCTACATTAAATGTTTTAGGAATAGATGAAATACCTTCAGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGCCCTGCAACTGGTGGCTACAATTATGACAATTTTATTGAAAATCCTAATAGTCCACAAGCAAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAAAGGTATCTCAGAAAATGCTGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNVDPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCPATGGYNYDNFIENPNSPQAKLFKKVSSIILKGISENAV", "aa_hexdigest": "c95348207eb1ab4dee7b8d8295cdda18", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11329.633999999987, "isoelectric_point": 4.478082847595215 }, "id": "LMJBGEANGM_259", "locus": "GCA013368015_00221" }, { "type": "cds", "sequence": "contig_1", "start": 125069, "stop": 125176, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCTAGGAGTTAAATTAGATAGTCAAAATAGTAGCAAATTGCAATCTTATTTAGACTATTACAATAATAATAAACAAGAAGCAGAAGGTGTTGATAGTATTTGA", "aa": "MALGVKLDSQNSSKLQSYLDYYNNNKQEAEGVDSI", "aa_hexdigest": "b5b929f33fbbdbfb23f365f34ac9e090", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3923.2309000000014, "isoelectric_point": 4.436874580383301 }, "id": "LMJBGEANGM_260", "locus": "GCA013368015_00222" }, { "type": "cds", "sequence": "contig_1", "start": 125252, "stop": 125443, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACCTAGAAACAAAAAACAGAAAAGATTTTCACAATATTTTATTAAATTACGCATCAGCAGCTGCTTTATATGCAAACCATAAAATGAATTTAGCGGTTGATGACAAAGATTTTAAGAAATATAACATCGAACCTGTATTTAATAATGCAAACTCTTTAATTGCAAATAATAAAGATAACAAAAAATGA", "aa": "MDLETKNRKDFHNILLNYASAAALYANHKMNLAVDDKDFKKYNIEPVFNNANSLIANNKDNKK", "aa_hexdigest": "54875efe247097299132ad564505a3ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7271.124200000002, "isoelectric_point": 8.964280509948733 }, "id": "LMJBGEANGM_261", "locus": "GCA013368015_00223" }, { "type": "cds", "sequence": "contig_1", "start": 125543, "stop": 125752, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTACCAAATTATTTACAGCAGTAGCAGCAGGATTATTTCCCACAACTGCATTAATTTCAGCCTCGTGCTCAACAACAGAAAATTCAAAATATGGATTTGACCAAGATGATGATGGACAATTAGTATTAGCAACAGGATTTTCAAAAAGTAACTGGCAAGGATTTGCCATTCAAGGAATTGTTAATACATATAATAATTGA", "aa": "MKKITKLFTAVAAGLFPTTALISASCSTTENSKYGFDQDDDGQLVLATGFSKSNWQGFAIQGIVNTYNN", "aa_hexdigest": "52f616b8c430d2ec34c886fc66c9d686", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7398.209100000002, "isoelectric_point": 5.952713584899903 }, "id": "LMJBGEANGM_262", "locus": "GCA013368015_00224" }, { "type": "cds", "sequence": "contig_1", "start": 126164, "stop": 126658, "strand": "+", "frame": 2, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "SO:0001217", "UniRef:UniRef50_Q9PPZ2", "UniRef:UniRef90_D1J7L7" ], "nt": "ATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGGAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCTCCTGGACAACACGGAGCAAAAAGAAGCAAACCATCAGACTATCAACTACACATGTACGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTACTACAAACTGTTGAATCAAGATTAGACAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCATGGACACTTTACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAATTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGATGCTATTGAAGTAATGACAGTTGCTCCATGA", "aa": "MSKFVGSMFKKSRRYGISLLGNNKEFTKGKKRTTAPGQHGAKRSKPSDYQLHMYEKQKVRYMYGLNERQFKNLFIKVSKKAGVTGINLLQTVESRLDNLVFRAGFARTRAQARQLVNHGHFTVDGCKANIPSMIVKVGSVIELKEALQNNVAIKDAIEVMTVAP", "aa_hexdigest": "877c3866aba651e3398c34a7f4d1d489", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7L7", "query_cov": 1.0, "subject_cov": 0.8241206030150754, "identity": 0.9940000000000001, "score": 314.0, "evalue": 1.1e-107, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "cog_id": "COG0522", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "LMJBGEANGM_263", "locus": "GCA013368015_00225" }, { "type": "cds", "sequence": "contig_1", "start": 126802, "stop": 127113, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63", "UniRef:UniRef90_UPI000AFE6B63" ], "nt": "TTGTTTACTTTTGTAATTATATTAATATATCTAATATCACTTATTTTGGGTATTCTTTTTTATCAAATTAGTCATGATAGACTCACAAACTATTTGATTATTGTTTTAAGTTCTATAGTTTTCCCTGAAATTTTAAATACGCTGTTTTCACTTTCGAATATAACAATGACGCTTAATCCTTTATGCAATAATATTTATCTAATTAATTTAGTTTATATACCCGCATTTATCTTATTTATTGTTTTAATTACAAAAACAAAAGAAAAACAATTTATATTTTTTAAAAAAGTTAATTATTTAAAAAATAAATAA", "aa": "MFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNPLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYLKNK", "aa_hexdigest": "2542b514f7902e4487960cf43ae8ee7b", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 126802, "stop": 127299, "observations": { "mutation": [ 127170, 127251 ], "stop": [ 127170, 127251 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFE6B63", "query_cov": 1.0, "subject_cov": 0.6242424242424243, "identity": 0.971, "score": 176.0, "evalue": 1.42e-54, "gene_start": 1, "gene_end": 103, "reference_start": 63, "reference_end": 165, "reference_sequence": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK" }, "paralog": false, "identity": 0.9272727272727272, "score": 297.0, "evalue": 7.09e-105, "description": "Internal stop codon at 127170, 127251. Nonsense mutation around 127170, 127251.", "psc": { "uniref90_id": "UniRef90_UPI000AFE6B63", "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFE6B63", "UniRef:UniRef50_UPI000AFE6B63" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12112.638799999979, "isoelectric_point": 9.519675636291506 }, "id": "LMJBGEANGM_264", "locus": "GCA013368015_00226" }, { "type": "cds", "sequence": "contig_1", "start": 127249, "stop": 127599, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q2Z3", "UniRef:UniRef90_D1J7L8" ], "nt": "TTGCTAGCAGGATTTGTAATTTATCTTTTAATATCAGCAATAATGTTAAAAATAGATGGTCAAATCGACCAATTTAATCTATTTCCTTTAATAAATCAAATATTTTTAATCGCGCCTTCTTATACTACCCAGCTTCTTTTAACATTATTATCAATAGGACTATTTTTAAACTTCTATTTTGTTAAACGATATTTATCAAAACGAAGCATAGAACTTGATCACAAAGAAAAAAGAAGAAACTATTTAATAATAATATTGACAACTGCAATATCGTTAATTGTGATATTTTTAATTAACGTAATGATTTTGTTTATTAAACAAAATTTTTATGAAATCGTCTCTGGACATTGA", "aa": "MLAGFVIYLLISAIMLKIDGQIDQFNLFPLINQIFLIAPSYTTQLLLTLLSIGLFLNFYFVKRYLSKRSIELDHKEKRRNYLIIILTTAISLIVIFLINVMILFIKQNFYEIVSGH", "aa_hexdigest": "c9ec08a87b3cb11df01df586c2f3a92d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 126946, "stop": 127663, "observations": { "insertion": [ 127642 ], "stop": [ 127170, 127251, 127605 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L8", "query_cov": 1.0, "subject_cov": 0.40559440559440557, "identity": 0.9740000000000001, "score": 207.0, "evalue": 5.07e-65, "gene_start": 1, "gene_end": 116, "reference_start": 22, "reference_end": 137, "reference_sequence": "MEKFVNEKNKKQLLLFLPYWILLAGFVIYLLVSAIMLKIDGQIDQFNLFPLINQIFLIAPSYTTQLLLTLLSIGLFLNFYFVKRYLSKRSIELDHKEKRRNYLIIILTTAISLIVIFLINVMILFIKQNFYKIVSGHWNTKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKIKEKQFIFFKKVNYAKNK" }, "paralog": false, "identity": 0.9663865546218487, "score": 432.0, "evalue": 2.42e-156, "description": "Internal stop codon at 127170, 127251, 127605. Frameshift due to insertion around 127642.", "psc": { "uniref90_id": "UniRef90_D1J7L8", "uniref50_id": "UniRef50_A0A6A8Q2Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L8", "UniRef:UniRef50_A0A6A8Q2Z3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q2Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q2Z3" ], "product": "ABC transporter permease" } }, "id": "LMJBGEANGM_265", "locus": "GCA013368015_00227" }, { "type": "cds", "sequence": "contig_1", "start": 127829, "stop": 128395, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L9", "UniRef:UniRef90_D1J7L9" ], "nt": "ATGATTTGTTCTAGATTACTATTGCCTTTGAATGATGTTGATGAATATAAATTAATTCCTCTTGTTAGAACAATCGAATTTACAATTTATACAAAGGCTTCAAAAATTAAACACGATAACGAAGTACTTCTAACTTCAATATCAAATAATTTAAGTCAATATGACATTGACAATTTTCAAGGTTTATATTGCGATATAAACCAAGCATTTGTTGCTGATAATCAATTATTTGACGAAGAAACAGAGTACCAATTTAAATTCAGCAACTCAAATGATGAAGATAATTACCAAGCAAGTTATATTATTCAAAAACTAATAAAAAAACTATTAAATTTTGTCAATGATGAAGACCTTAATTATTGCTTTATTATTATGACAAAAATCCAAAATAATATTATCAAACCTTTTTATATATATTGCAACCCAGAAGATGCAAAAAAAGAACTTGAACAACTATTTAAAACATTGGATAATACAAAATACGAAGCATTACTATTAGAAGCAGCAAATACTTTTAGTTTCGAATTAAAAAAATTTAATGAAGAATATTTAAACAAATCTAGTTGA", "aa": "MICSRLLLPLNDVDEYKLIPLVRTIEFTIYTKASKIKHDNEVLLTSISNNLSQYDIDNFQGLYCDINQAFVADNQLFDEETEYQFKFSNSNDEDNYQASYIIQKLIKKLLNFVNDEDLNYCFIIMTKIQNNIIKPFYIYCNPEDAKKELEQLFKTLDNTKYEALLLEAANTFSFELKKFNEEYLNKSS", "aa_hexdigest": "45fb82665d3b0212c51064835bf49ec0", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J7L9", "query_cov": 1.0, "subject_cov": 0.8663594470046083, "identity": 0.9890000000000001, "score": 365.0, "evalue": 2.08e-127, "valid": true, "uniref50_id": "UniRef50_D1J7L9" }, "pscc": { "uniref50_id": "UniRef50_D1J7L9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22208.898600000022, "isoelectric_point": 4.583007621765137 }, "id": "LMJBGEANGM_266", "locus": "GCA013368015_00228" }, { "type": "cds", "sequence": "contig_1", "start": 128698, "stop": 128961, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63", "UniRef:UniRef90_UPI000AFE6B63" ], "nt": "TTGTTTTATCAAATTAGCCATGATAGATTTTTAAACTACCTAATTATTATTTTGAGTTCCATTGTTTTTCCCGAAATTTTAAACTCTATATATTCACTTTCAAATATAACAATGACACTTGGTTCTTTATACAATAATATTTATCTAATAAATTTAGTTTATATACCAGCATTTGTCTTATTTATTGTTTTAATCGCAAAAATCAAAGAAAAAAATTTCATATTTTTTAAGAAATTTAATACCATTAAAAATAAGAAACTATAA", "aa": "MFYQISHDRFLNYLIIILSSIVFPEILNSIYSLSNITMTLGSLYNNIYLINLVYIPAFVLFIVLIAKIKEKNFIFFKKFNTIKNKKL", "aa_hexdigest": "2e39be25f8322371e0db30001c842a47", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 128704, "stop": 129201, "observations": { "mutation": [ 129072 ], "stop": [ 129072 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFE6B63", "query_cov": 0.9770114942528736, "subject_cov": 0.5151515151515151, "identity": 0.812, "score": 127.0, "evalue": 7.4e-36, "gene_start": 1, "gene_end": 85, "reference_start": 81, "reference_end": 165, "reference_sequence": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK" }, "paralog": false, "identity": 0.8666666666666667, "score": 285.0, "evalue": 2.89e-100, "description": "Internal stop codon at 129072. Nonsense mutation around 129072.", "psc": { "uniref90_id": "UniRef90_UPI000AFE6B63", "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFE6B63", "UniRef:UniRef50_UPI000AFE6B63" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10302.309199999985, "isoelectric_point": 9.744219017028808 }, "id": "LMJBGEANGM_267", "locus": "GCA013368015_00229" }, { "type": "cds", "sequence": "contig_1", "start": 129070, "stop": 129201, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTATTTATTAATCAAAGTTATTATAGAATAAGGGATGGAAGGGATCATTCAAAAATTCCTGTATATTTAGAAAATACAAAAATTATTGAATATTTTTATGTTTATATTTTTAGATTAGCTTATTGA", "aa": "MILFINQSYYRIRDGRDHSKIPVYLENTKIIEYFYVYIFRLAY", "aa_hexdigest": "3bc05c95c6824f7aacdeea0b080cfa07", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5426.2499, "isoelectric_point": 9.098697090148928 }, "id": "LMJBGEANGM_268", "locus": "GCA013368015_00230" }, { "type": "cds", "sequence": "contig_1", "start": 129307, "stop": 129546, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DC110", "UniRef:UniRef90_UPI001E2DC110" ], "nt": "ATGAAAACTAAAAAACAATTATTAATATTTTTACCATATTTAATATTACTAGGTGGTTTTACAATATATCTTTTAATATCATCAATAATATTAAAAGTAGATGGTGAGATTGACAATACTAATTTATTTAATTTAATTAATCAAATATTTTTGATTGCCCCGTCATATACTACGCAACTTCTTTTAATGTTGTTATCAATAGGACTCTTTTTAAACTTTTATTTTGCTAAGAGATATTGA", "aa": "MKTKKQLLIFLPYLILLGGFTIYLLISSIILKVDGEIDNTNLFNLINQIFLIAPSYTTQLLLMLLSIGLFLNFYFAKRY", "aa_hexdigest": "c674b695fc57aeedb33bae278a490505", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 129221, "stop": 129549, "observations": { "insertion": [ 129266, 129273 ], "mutation": [ 129309 ], "stop": [ 129309 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2DC110", "query_cov": 0.9873417721518988, "subject_cov": 0.7222222222222222, "identity": 0.9620000000000001, "score": 140.0, "evalue": 1.31e-41, "gene_start": 2, "gene_end": 79, "reference_start": 3, "reference_end": 80, "reference_sequence": "MKKNKKQLLLFLPYWILLGGFTIYLLISSIILKVDGEIDNTNLFNLINQIFLIAPSYTTQLLLMLLSIGLFLNFYFAKRYWTKHNIELDFKEKENLFNNNINNYNCIN" }, "paralog": false, "identity": 0.9444444444444444, "score": 193.0, "evalue": 4.23e-65, "description": "Internal stop codon at 129309. Frameshift due to insertion around 129266, 129273. Nonsense mutation around 129309.", "psc": { "uniref90_id": "UniRef90_UPI001E2DC110", "uniref50_id": "UniRef50_UPI001E2DC110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2DC110", "UniRef:UniRef50_UPI001E2DC110" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2DC110", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DC110" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9150.005599999993, "isoelectric_point": 9.398346138000488 }, "id": "LMJBGEANGM_269", "locus": "GCA013368015_00231" }, { "type": "sorf", "sequence": "contig_1", "start": 129730, "stop": 129756, "strand": "-", "gene": "mgrB", "product": "MgrB", "frame": 3, "db_xrefs": [ "SO:0001217", "UniParc:UPI00051CBDFA", "UniRef:UniRef100_A0A097F1Q3" ], "aa": "MKKLRWFY", "aa_hexdigest": "240d3d40d40b4070f9b94efaad3a16ee", "nt": "ATGAAGAAACTCAGATGGTTCTACTAA", "ups": { "uniparc_id": "UPI00051CBDFA", "uniref100_id": "UniRef100_A0A097F1Q3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00051CBDFA", "UniRef:UniRef100_A0A097F1Q3" ] }, "ips": { "uniref100_id": "UniRef100_A0A097F1Q3", "product": "MgrB" }, "genes": [ "mgrB" ], "id": "LMJBGEANGM_1292", "locus": "GCA013368015_00232" }, { "type": "cds", "sequence": "contig_1", "start": 129971, "stop": 130228, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGTAGGATATGTAGATCAAACTTTTTTGAGTGATAAATACAATGATACTTCTGGATTATGTGAATATGTTGCAATATCAAATATTTTACTTTATAATGAACTTTTTAATCAAAGTGGTATTTTTACAAATGAAGAATTTAATGATTTTTTTAAATATGATAATTATGACGATTCTATAGAAAATTCATCGCCTATTTTTAGATATTATCAATATGATCATCCTGATTATTCATTAGTTGCTAAATTATGA", "aa": "MQKVGYVDQTFLSDKYNDTSGLCEYVAISNILLYNELFNQSGIFTNEEFNDFFKYDNYDDSIENSSPIFRYYQYDHPDYSLVAKL", "aa_hexdigest": "0d67e6e40e368d7602e99592bf5931ae", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10088.896599999993, "isoelectric_point": 4.08794002532959 }, "id": "LMJBGEANGM_270", "locus": "GCA013368015_00233" }, { "type": "cds", "sequence": "contig_1", "start": 130244, "stop": 130675, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAAATAAAAATTCCTGCATTAACCATAGTAGCAGCAATGCCTTGTTGCTTATTGTCTAGCAATATTAACAACAATGAAAACTATAAAGAATTAAAAGAAAAAGTAACCAATACTATTCAAAGATTTTTTGGAAAAAATCAACAATTTAAATATATAAAAAATCTAAATAATGAATATTTATACATTCAATGTTCTAACTATTATGCTATCGTTGATAAAAACGATTTTTCAATTACTGAAATCAAAAAAGGTTTTCATTCTTATAATGAAAACATAAAATATAATTTTAATTATTTAGAAAAAGAAAATAAAAACTTTAATGAATTAAGAATGGCATATAAGAAAGATTATTTTTCTCCAGTTTTGGAAGACAAACTAGATACATTGCCAAATATAAGCAATATGGTTAAAAATAGTTGGTGGTGA", "aa": "MMKIKIPALTIVAAMPCCLLSSNINNNENYKELKEKVTNTIQRFFGKNQQFKYIKNLNNEYLYIQCSNYYAIVDKNDFSITEIKKGFHSYNENIKYNFNYLEKENKNFNELRMAYKKDYFSPVLEDKLDTLPNISNMVKNSWW", "aa_hexdigest": "dd160841b06cab319db7508b42d042e0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17132.473099999996, "isoelectric_point": 8.98233165740967 }, "id": "LMJBGEANGM_271", "locus": "GCA013368015_00234" }, { "type": "cds", "sequence": "contig_1", "start": 130908, "stop": 131075, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_162980102.1", "SO:0001217", "UniParc:UPI000305A2B6", "UniRef:UniRef100_UPI000305A2B6", "UniRef:UniRef50_UPI000305A2B6", "UniRef:UniRef90_UPI000305A2B6" ], "nt": "ATGAATGACAAAAATAATAAAGTAAGTGGAAACACACATACAAGACAACAAATGAATAATTATTCAAATCAAAATAATTCTAATAATTCAGCATACAGATCAAATTTAAACAATCATTCTAATCAATTGAACCCAAACAATAGCAACTACAAGGGCAACAAAAAATAA", "aa": "MNDKNNKVSGNTHTRQQMNNYSNQNNSNNSAYRSNLNNHSNQLNPNNSNYKGNKK", "aa_hexdigest": "4701f2ca3e730026670d7ff586fbd515", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000305A2B6", "ncbi_nrp_id": "WP_162980102.1", "uniref100_id": "UniRef100_UPI000305A2B6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000305A2B6", "RefSeq:WP_162980102.1", "UniRef:UniRef100_UPI000305A2B6" ] }, "ips": { "uniref100_id": "UniRef100_UPI000305A2B6", "uniref90_id": "UniRef90_UPI000305A2B6" }, "psc": { "uniref90_id": "UniRef90_UPI000305A2B6", "uniref50_id": "UniRef50_UPI000305A2B6" }, "pscc": { "uniref50_id": "UniRef50_UPI000305A2B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000305A2B6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6326.585400000004, "isoelectric_point": 10.214580345153806 }, "id": "LMJBGEANGM_272", "locus": "GCA013368015_00235" }, { "type": "cds", "sequence": "contig_1", "start": 131247, "stop": 131423, "strand": "-", "frame": 1, "gene": null, "product": "putative dsRNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC", "UniRef:UniRef90_UPI00215D72BC" ], "nt": "ATGTTAGATATTGAACATTTTTTTAGAGATGTTGATTGCAGAGAAAAAAGACCGGATAAATTTAAATTAGAAAATGCTATCAATACATTAAAAGAATTAGCAGAGCGTGGTGAATGCTCAATGCCAATATATCAAATATCAGAAAAACCTATAATTGATAATAACGGTAAACAATGA", "aa": "MLDIEHFFRDVDCREKRPDKFKLENAINTLKELAERGECSMPIYQISEKPIIDNNGKQ", "aa_hexdigest": "44cbf3cae5dce09ce5b908de8959d715", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 131085, "stop": 131513, "observations": { "mutation": [ 131207, 131246, 131249, 131459 ], "stop": [ 131207, 131246, 131249, 131459 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D72BC", "query_cov": 1.0, "subject_cov": 0.4084507042253521, "identity": 0.948, "score": 116.0, "evalue": 4.09e-32, "gene_start": 1, "gene_end": 58, "reference_start": 31, "reference_end": 88, "reference_sequence": "MKADLFEAIIGAIAIDSGWDIKRLKNIIIKMLDIERFFRDVDCREKRPNKFKLENAINTLKELAERGECSMPIYQISEKPIIDDNGKQWWHCRCNVNFRNLQWMGLWTKYTEGYGTSKKLAKKMAAYNMLCDHYGLPNEYKN" }, "paralog": false, "identity": 0.9366197183098591, "score": 270.0, "evalue": 1.92e-95, "description": "Internal stop codon at 131207, 131246, 131249, 131459. Nonsense mutation around 131207, 131246, 131249, 131459.", "psc": { "uniref90_id": "UniRef90_UPI00215D72BC", "product": "putative dsRNA-binding protein", "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D72BC", "UniRef:UniRef50_UPI00215D72BC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC" ], "product": "putative dsRNA-binding protein" } }, "id": "LMJBGEANGM_273", "locus": "GCA013368015_00236" }, { "type": "cds", "sequence": "contig_1", "start": 131457, "stop": 131933, "strand": "-", "frame": 1, "gene": null, "product": "RNase III domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G3X3E2" ], "nt": "ATGGGAATAGAAGAATGGCAAGAATATATTTATCAAATTGAATATACAATAGGATATTTCTTCAAAAATAAATTTTTACTTTATCAAGCATTTACTAGAAGTTCTTATTCAAATCAATATGGAGTTGAAAATAATGAAGTTCTTGAATTTATAGGAGATTCAGTTTTGAGCTTTTGCGTAGTTCAACTAATGGCAGAAGAATTTGGCTTTTTAAAATCGGCTTCGCAATATTACAACAAATATGAAGATCTTGAAGAATATTGCCTTATAGCCAATAAAAATGAATCCGATTTTACTGATTTAAAAAATCAAATCGTAAGCAATAAAACACTAGCTAAAAAAATTAATAAATTAGGTTTTGCTAAGTATTTAGTTTTAGGAGATAATGATAAAAGAAATAATGTTGCTAATCAAGATAGTGTGAAAGCTGATTTATTTGAAGCTATAATTGGTGCAATAGCAATTGACAGTGGTTGA", "aa": "MGIEEWQEYIYQIEYTIGYFFKNKFLLYQAFTRSSYSNQYGVENNEVLEFIGDSVLSFCVVQLMAEEFGFLKSASQYYNKYEDLEEYCLIANKNESDFTDLKNQIVSNKTLAKKINKLGFAKYLVLGDNDKRNNVANQDSVKADLFEAIIGAIAIDSG", "aa_hexdigest": "504609435cd77ff20038208487c1736f", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A2K9A1I8", "query_cov": 0.9810126582278481, "subject_cov": 0.8908045977011494, "identity": 0.535, "score": 162.0, "evalue": 3.4e-48, "valid": false, "product": "RNase III domain-containing protein", "uniref50_id": "UniRef50_A0A1G3X3E2" }, "pscc": { "uniref50_id": "UniRef50_A0A1G3X3E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1G3X3E2" ], "product": "RNase III domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_274", "locus": "GCA013368015_00237" }, { "type": "cds", "sequence": "contig_1", "start": 132156, "stop": 132521, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTTCTTTTATTTAAAAATAAATTTAAGCGTATATATTAATTTTTGCAAAATAATATTGCAATGTGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTGCCAAACTCAAAGCCAATGCCTGCAATATTAAATGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGATTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAGTAAAGAAGAATTATTTTTAATTGATAGATGA", "aa": "MTFFYLKINLSVYINFCKIILQCEFYLILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKPMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEISKEELFLIDR", "aa_hexdigest": "5bc1067ade02536ba69f326dcc43b66a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14327.689899999983, "isoelectric_point": 5.096888923645018 }, "id": "LMJBGEANGM_275", "locus": "GCA013368015_00238" }, { "type": "cds", "sequence": "contig_1", "start": 132648, "stop": 133106, "strand": "+", "frame": 3, "gene": null, "product": "Putative gamma-glutamylcyclotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5", "UniRef:UniRef90_A0A2K9YSY5" ], "nt": "ATGGGATTTTCTAGAAATCCTAATGCCAATGAAAATAATTTAAATGCTTTTATTGAAATGGAAAAGGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGATATAAACGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCTTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCACGGATCAATTTTTGCAATTAAAGAAGATGGTAAAATGTATGCAGCCTTAACTTCGTTTAGCCAAAAATCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCTGAAATAACGTTAAAATCGTTATACGATAATACAAATATAGATTTTTTAATTAATAAAAAGCCTGTTTATTATTTAAGTTCAAGAGAAGACAAAACAATCAATGAAACTCAAGTTGGTTGA", "aa": "MGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYAALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKTINETQVG", "aa_hexdigest": "2bb34cbae2fa7cabb3d54e4c608c0fbd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 132348, "stop": 133220, "observations": { "mutation": [ 132519, 133104 ], "stop": [ 132519, 133104 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2K9YSY5", "query_cov": 1.0, "subject_cov": 0.45645645645645644, "identity": 1.0, "score": 303.0, "evalue": 8.1e-102, "gene_start": 1, "gene_end": 152, "reference_start": 144, "reference_end": 295, "reference_sequence": "QYEFYLILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKQMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEISKEELFLIDRWELFPQYQRFQVNVLLTETNEILENVYVYTKLEVGKYYLATDDMGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYAALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKTINETQVGWYENKAFELVEKDFDIDPFIRFNKMLKAFFDTKQKNDK" }, "paralog": false, "identity": 0.9896551724137931, "score": 572.0, "evalue": 4.2e-210, "description": "Internal stop codon at 132519, 133104. Nonsense mutation around 132519, 133104.", "psc": { "uniref90_id": "UniRef90_A0A2K9YSY5", "product": "Putative gamma-glutamylcyclotransferase", "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2K9YSY5", "UniRef:UniRef50_A0A2K9YSY5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5" ], "product": "Putative gamma-glutamylcyclotransferase" } }, "id": "LMJBGEANGM_276", "locus": "GCA013368015_00239" }, { "type": "cds", "sequence": "contig_1", "start": 133327, "stop": 134916, "strand": "+", "frame": 1, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9", "UniRef:UniRef90_D1J7M2" ], "nt": "ATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCCGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTACAAGCCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGATATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCTGACAATAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAAGATGGAATTATAATTGAAAAACAAAGTCCCGAATTTGCAAAGCAGAGATTTGAACAAGCCAAATCAATCATGAAATTTGGTCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAAATGAACCTGTTTGAGCAGGCGAGATTTTATGACCAAATTTTAGATTTGTTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAATATCAAAACTATATGTTGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTATGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTTATTGATGAATTTTATGTTAATAATCAATTGCCCGATCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAATGACAAAGTAAGCGCGCCATTGAATAAATTAAATAAAAACTTGATCGATATTGCTATTGAGAATGCATCAAATAATATTGAACCAAAAATAAAAAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCGATAAATTATGTTCATATTTGAATGTTTATGCAGAAAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGAGCAGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCAGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTTGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGCTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAATAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAAGTTGATAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATTAATTATTTTGGCAGTTATAAAAAAATTCAACAAGCTTCTTTTGATGAAATATCAAAGGTGGCAAACAAAAAAGTTGCTAAGCTAATTTGTGAAGACAAAGGCGAATCGTTTTATTAA", "aa": "MLQYFDIKMQNSFKTEKMRQEIADFSTIILSSEREAFIEERKLIDFYKPFYNVLFPTRDSFPYIRVKLLSTNKLIIDIKNHYKKEPNSIYYGPIPDNKNFKPLIRYLNHLLLSKDGIIIEKQSPEFAKQRFEQAKSIMKFGPDFKRNLKQKINEAIEMNLFEQARFYDQILDLLNYNKQSQNIFIKTIKDIDVFGFYQYQNYMLVNVMIYKNGSLVNSYDYIFEIILNEHEFVNQFIDEFYVNNQLPDQIIIPLKYYELGGFDYNDKVSAPLNKLNKNLIDIAIENASNNIEPKIKNYLSKQTKDNEIFDKLCSYLNVYAERIVLFDNSFLSNTNEVIGAACYFKNGQAFKKFYRFYNLKLNLLRNADVEYMQQTSFNYLKDFGDQVDVVIVDGSIGQINEVKKSMAFLNLNIPLFGLVKNSKHETQKLIDSNNNEIKIDDQDVFNLLSRMQTEVDRFAKYRYNQKQRKSMLSNPLLKIKGIGFKTIEKLINYFGSYKKIQQASFDEISKVANKKVAKLICEDKGESFY", "aa_hexdigest": "71cb9a6c55841bcd4a32ac6fe9c3eb0e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M2", "query_cov": 1.0, "subject_cov": 0.921602787456446, "identity": 0.987, "score": 1014.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_A0A2Z5IPW9", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IPW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9" ], "product": "Excinuclease cho" }, "genes": [ "uvrC" ], "id": "LMJBGEANGM_277", "locus": "GCA013368015_00240" }, { "type": "tRNA", "sequence": "contig_1", "start": 134981, "stop": 135056, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 76.3, "nt": "GGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 135014, 135016 ], "id": "LMJBGEANGM_4", "locus": "GCA013368015_00241" }, { "type": "tRNA", "sequence": "contig_1", "start": 135126, "stop": 135199, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 71.2, "nt": "GCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 135158, 135160 ], "id": "LMJBGEANGM_5", "locus": "GCA013368015_00242" }, { "type": "tRNA", "sequence": "contig_1", "start": 135202, "stop": 135275, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 70.8, "nt": "GCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 135234, 135236 ], "id": "LMJBGEANGM_6", "locus": "GCA013368015_00243" }, { "type": "cds", "sequence": "contig_1", "start": 135720, "stop": 136136, "strand": "+", "frame": 3, "gene": "azoR", "product": "FMN dependent NADH:quinone oxidoreductase", "db_xrefs": [ "COG:C", "COG:COG1182", "EC:1.7.1.17", "KEGG:K01118", "SO:0001217", "UniRef:UniRef50_D1J7M3", "UniRef:UniRef90_D1J7M3" ], "nt": "ATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAAAAATAATATTAACGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCCTTAACTGACAAGAACTTTAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTGCAATTAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATTGCAACGCAAGGAGCTCCATTAGGTTGA", "aa": "MAKVLIIYGSVIPRENSISTKMLDLFVDEYKKNNINDEIKILDLNNEDMYSLTDKNFKLFYSEENSDKYINLLKSVDKVVMNAPMINFNIPTAIKAFFDRIAVANKTFSYKYASKGSSIGLLNNLKVQIIATQGAPLG", "aa_hexdigest": "a2671a55d9a65b4f5839c1a9106e143c", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "azoR" ], "truncated": "3-prime", "pseudogene": { "start": 135720, "stop": 136313, "observations": { "mutation": [ 136134, 136143, 136176 ], "stop": [ 136134, 136143, 136176 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M3", "query_cov": 1.0, "subject_cov": 0.700507614213198, "identity": 0.993, "score": 262.0, "evalue": 9.33e-88, "gene_start": 1, "gene_end": 138, "reference_start": 1, "reference_end": 138, "reference_sequence": "MAKVLIIYGSVIPRENSISTKMLDLFVDEYKKNNINDEIKILDLNNEDMYSLTDKNFKLFYSEENSDKYINLLKSVDKVVMNAPMINFNIPTIIKAFFDRIAVANKTFSYKYASKGSSIGLLNNLKVQIIATQGAPLGWYPWASHINYLEGMWKFLGANLSESILLAGTKVEPLNKMQPQEIIETISKEIIKKASIF" }, "paralog": false, "identity": 0.9796954314720813, "score": 371.0, "evalue": 5.08e-133, "description": "Internal stop codon at 136134, 136143, 136176. Nonsense mutation around 136134, 136143, 136176.", "psc": { "uniref90_id": "UniRef90_D1J7M3", "gene": "AzoR", "product": "FMN dependent NADH:quinone oxidoreductase", "ec_ids": [ "1.7.1.17" ], "uniref50_id": "UniRef50_D1J7M3", "cog_id": "COG1182", "cog_category": "C", "kegg_orthology_id": "K01118", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M3", "EC:1.7.1.17", "UniRef:UniRef50_D1J7M3", "COG:COG1182", "COG:C", "KEGG:K01118" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7M3" ], "product": "FMN dependent NADH:quinone oxidoreductase" } }, "id": "LMJBGEANGM_278", "locus": "GCA013368015_00244" }, { "type": "cds", "sequence": "contig_1", "start": 136416, "stop": 136838, "strand": "+", "frame": 3, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "SO:0001217", "UniRef:UniRef50_B3PM28", "UniRef:UniRef90_D1J7M4" ], "nt": "ATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCCGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATATAAATTACCTGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGA", "aa": "MAKKVVKKAKLQFAAGQAKPGPALAGVGINMPEFTKAFNDKTRERGNEPVPVLITVYEDKSFEFKLFTSPTSYKLIEAAKIKKGSGVANKEKVGSITIDQLRAIAEYKLPDLNTTKIDSAMKQVAGTAKNMGITVEGYDE", "aa_hexdigest": "c988e0254890aaae286c794b34012d8d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7M4", "query_cov": 1.0, "subject_cov": 0.9523809523809523, "identity": 1.0, "score": 271.0, "evalue": 8.2e-92, "valid": true, "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_B3PM28", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM28" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "LMJBGEANGM_279", "locus": "GCA013368015_00245" }, { "type": "cds", "sequence": "contig_1", "start": 136859, "stop": 137551, "strand": "+", "frame": 2, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_012855365.1", "SO:0001217", "UniParc:UPI0001BC20A4", "UniRef:UniRef100_D1J7M5", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_D1J7M5" ], "nt": "ATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTCCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCCGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTACTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCTGATCCTAAAATGATGTTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAGGCCGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGCGGAATTGTTCATGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTTTAGCAGAAAATGCTGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAGGGTACATATGTTTTAAATATTACCGTTTCAACTTCTATGGGTCAATCTGTTAAAGTAAGAATTGATTAA", "aa": "MAKRITKNIKNVKSLVNKKDVFPLADAIELAKKASFAKFDESVDIAIRLNLDTRKSDQQLRGSVVLPHGTGKSVKVLVATDEVSAQKASLEAGADFVYSSAELPEVLNQDKYDFDVIVADPKMMLVLGKFGKKLGPKGLMPNPKTGTVTINPAKAVEELKKGKANYRADKGGIVHASFGKKSMETNVLAENADTIIQTIKRLKPQTVKGTYVLNITVSTSMGQSVKVRID", "aa_hexdigest": "b6b4b4b8e99890894c640bc5c2c3664f", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC20A4", "ncbi_nrp_id": "WP_012855365.1", "uniref100_id": "UniRef100_D1J7M5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20A4", "RefSeq:WP_012855365.1", "UniRef:UniRef100_D1J7M5" ] }, "ips": { "uniref100_id": "UniRef100_D1J7M5", "uniref90_id": "UniRef90_D1J7M5" }, "psc": { "uniref90_id": "UniRef90_D1J7M5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "LMJBGEANGM_280", "locus": "GCA013368015_00246" }, { "type": "cds", "sequence": "contig_1", "start": 137662, "stop": 138054, "strand": "-", "frame": 3, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGAATAAGAGATTTGGCAGCAAAGTTCAACTAGTTGGAGATGATTTAATTGTAACAAATCCAAAATACATTCAAATGGCTATTGACAAACATGCAATCAATGCATCTTTAATAAAGATAAATCAAATAGGTTCATTAACTGAAACATTTAAAGCAATAACAATGTCACAAAAAGCTGGCTTTGTAGCAATAATTTCTCACCGTTCAGGTGAAACTGAAGATACATTTATTGCAGATTTGGCAGTAGCATTAAATACTGGGGAAATCAAAACTGGCTCTCTTTCAAGAACAGATAGAATTGCTAAATACAATCGCTTGTTAAAAATAGAAGCAGAATTAGGCAATGAAGCTAAATTTGAAGGCAAAAACTCATTTTATAATCTAAAAAATTAA", "aa": "MNKRFGSKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIEAELGNEAKFEGKNSFYNLKN", "aa_hexdigest": "518bcdd9bdc09b8037f89afadf85377b", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 0.9923076923076923, "subject_cov": 0.8376623376623377, "identity": 0.636, "score": 169.0, "evalue": 1.63e-51, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "LMJBGEANGM_281", "locus": "GCA013368015_00247" }, { "type": "cds", "sequence": "contig_1", "start": 138070, "stop": 138837, "strand": "-", "frame": 3, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P0DXB0", "UniRef:UniRef90_D1J7M6" ], "nt": "GTGCAATTAGCATGCGATAATGTTAATAATATTATTGCTAAAGCTATTATTGGCAAGGAAGTTTCTAAGCAAGAAGAATTAGATAATTTAATGATTAATCTTGATGGAACTGAAACAAAATCAAAATTAGGGGCAAATGCAATATTAGCTGTTTCATTAGCAATTGCAAAAGCTGGTGCCATTGAAGCAAATCTTCCATTATATAAATACTTGGCAAAATTAAACAACCAAGTAGCATACAAAATGCCAGTGCCAATGTTAAACGTTATTAATGGTGGAGAACATGCTTCAAATACTATTGACTTCCAAGAATTTATGATAATGCCTATTGGGGCAAAAACATTTAAAGAATCATTGCAAATGGCAAACTTCGTATTTCATACATTAGCAAAATTATTGAAACAAGCAGGTCATGGAACACAAGTTGGAGATGAAGGTGGATTTGCACCAAACCTACACACTCATGAAGAAGCATTGGACTTTTTGGTAAATGCAATTAAAGTCGCAGGATATAATCCAGCCACTTCAGGAGAAAAAGCAGTAGCAATTTGTTTGGATGCTGCAAGTTCAGAACTTTATCATTCAGATACAAAGTTATATGTATTTAAAAAATTCAAGAAAGCAATTGAAGAAAAACGCCCAGGATTTGAAAAATATCAAAATGTTAAATATTCATTTACTAGCGATGAATTTGTTGAATATTATGGCCATTTAATTGAAAAATACCCAATAATTTCTATTGAAGATTCACATGATGAAAACGATTGA", "aa": "MQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDEND", "aa_hexdigest": "bfac1696c8cd4a683586cc12527d4998", "start_type": "GTG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 137767, "stop": 139035, "observations": { "mutation": [ 138072, 138855 ], "stop": [ 138072, 138855 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M6", "query_cov": 1.0, "subject_cov": 0.5579868708971554, "identity": 0.996, "score": 495.0, "evalue": 7.28e-174, "gene_start": 1, "gene_end": 255, "reference_start": 67, "reference_end": 321, "reference_sequence": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENNWFGGKGVQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDENDWDGFIKMNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIESELGNEAKFEGKNSFYNLKN" }, "paralog": false, "identity": 0.9928909952606635, "score": 818.0, "evalue": 1.67e-303, "description": "Internal stop codon at 138072, 138855. Nonsense mutation around 138072, 138855.", "psc": { "uniref90_id": "UniRef90_D1J7M6", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P0DXB0", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M6", "EC:4.2.1.11", "UniRef:UniRef50_P0DXB0", "COG:COG0148", "COG:G", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P0DXB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0DXB0" ], "product": "Enolase" } }, "id": "LMJBGEANGM_282", "locus": "GCA013368015_00248" }, { "type": "cds", "sequence": "contig_1", "start": 138853, "stop": 139035, "strand": "-", "frame": 3, "gene": null, "product": "Phosphopyruvate hydratase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "nt": "ATGTCAAAAATTATTAAGATTAATGCCTATGAAGTTTTAGATAGTAGAGGAAATCCAACAGTTAAAGTTGAATTAAATACTGAACTTGCATATTCTGAAGCATTAGTACCATCAGGAGCTTCAACAGGATCAAAAGAAGCACTTGAATTACGCGACAAAGGAACAAAATACGAAAATAATTGA", "aa": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENN", "aa_hexdigest": "6327a23b922b52ad6b82638088f018a6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A920A3H1", "query_cov": 0.9166666666666666, "subject_cov": 0.873015873015873, "identity": 0.527, "score": 55.1, "evalue": 8.27e-09, "valid": false, "product": "Enolase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A7V5C5X0" }, "pscc": { "uniref50_id": "UniRef50_A0A7V5C5X0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "product": "Phosphopyruvate hydratase" }, "genes": [], "id": "LMJBGEANGM_283", "locus": "GCA013368015_00249" }, { "type": "cds", "sequence": "contig_1", "start": 139239, "stop": 139820, "strand": "+", "frame": 3, "gene": null, "product": "DUF3196 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90", "UniRef:UniRef90_D1J7M7" ], "nt": "ATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAAATTGAAAAAATTGCAAAAGAAGACCCACTTAAAGCAATTTATATTTTAGATGAAGACAAAAAACAATTCTCTCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAGTTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAATAGTTTAAAAAACAAAAAAATGGGTTATGCATTTATGCTAAGCTACAACGAACTTAAAAAACGAGATGATATAGCGAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGCAATATTGATTTTGAATACGATATTCAAAATAAAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAGAATGCTGAAATTCAAAAGATGCAAGATGAAATTATTGCAACTTTTGATAAAGATATTTCAAAAAACAAGGTTGCAAGGCAAGTATTTTCAGCGTATTTATTTCAAAATTGA", "aa": "MNNIDNNLFFIEEEIKKIEKIAKEDPLKAIYILDEDKKQFSLKEDLDDLDALRDNIEFQLKKQNLITSTKLDTLSLINSLKNKKMGYAFMLSYNELKKRDDIAKYAIEFQDFFKGNDFDSKGFQTLIYDLLVNSNIDFEYDIQNKKINPKKLGSLLKNAEIQKMQDEIIATFDKDISKNKVARQVFSAYLFQN", "aa_hexdigest": "f99745e6473616439ba34ca33c613926", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M7", "query_cov": 1.0, "subject_cov": 0.8143459915611815, "identity": 1.0, "score": 367.0, "evalue": 1.81e-127, "valid": true, "uniref50_id": "UniRef50_A0A3G8LJ90" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90" ], "product": "DUF3196 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_284", "locus": "GCA013368015_00250" }, { "type": "cds", "sequence": "contig_1", "start": 139833, "stop": 139952, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACGCCAGATTCACTTTCCAAAGAAGAAAAAGAGTTATATAACATTTTTAAATAA", "aa": "MHETKDDYLKISHVIEVILGNQTPDSLSKEEKELYNIFK", "aa_hexdigest": "026cff8c11c43173654580bfaff0a607", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4606.1672, "isoelectric_point": 5.079553031921385 }, "id": "LMJBGEANGM_285", "locus": "GCA013368015_00251" }, { "type": "cds", "sequence": "contig_1", "start": 140193, "stop": 141425, "strand": "+", "frame": 3, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_A0A501XAI0", "UniRef:UniRef90_D1J7M8" ], "nt": "TTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGACGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTACAAAAAATTGGGTGTTAAATTAGGATCATGTGAATTAACTAAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGACGGCGGAGAAGCTGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATTGGCCTAAAAGCAGGAGAAGAAAAAGATTTAGATTTAGTATTTCCAGAAGATTATCATGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTTGTTAAAACTCCAAATTATCCAACAATCGATGATAATTTTGTAAAGGAAATTAATTTGCCAAATGTTAAAAACGTTGCTGAATTTGAAGAATATGTAAAATTTATTGCTTTAAAGAACAAATTGGCAAATGTTAAAAATGAATTTATTAATGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAGGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTACTGAAAAAGATTATTACGAATTTTCAAAGAGCACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAACAAATGTTTGTATTAGGCGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAACATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGCGAAATTTTACTAAAAGAAAATGACACAAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGATGAAGCTAAACGTAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAA", "aa": "MDSILDNIYKEQIISQITKDDEIVGHANLRIKDINEEKAEISFVFPLFPEVKLGDYKKLGVKLGSCELTKKDLNDAKSQIASNYVVMLDSKEPIKEKDQVNFNFKGFIDGKPFDGGEAENFDLVIGSKQFIPGFEDKMIGLKAGEEKDLDLVFPEDYHAKNLAGKSVVFHVKINFVKTPNYPTIDDNFVKEINLPNVKNVAEFEEYVKFIALKNKLANVKNEFINEAVKKLSEQSEVSLSESLVEEEADKYYQNFLNNLKQQQITEKDYYEFSKSTKEESLNVFKKQAQINLKQMFVLGAIAKAESLKVTLEDYEKEVEKLAKTYGLAPEHVKQILKFENVENNLINERISEILLKENDTKAFENFDKLLKEVEEYEAKQTQAIVDEAKRKREEAEKIAKESKENIDEQK", "aa_hexdigest": "017d13de54f2c5265ef1d4f8a0884fbf", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M8", "query_cov": 1.0, "subject_cov": 0.8613445378151261, "identity": 0.998, "score": 762.0, "evalue": 3.51e-276, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_A0A501XAI0", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XAI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XAI0" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "LMJBGEANGM_286", "locus": "GCA013368015_00252" }, { "type": "cds", "sequence": "contig_1", "start": 141517, "stop": 142743, "strand": "+", "frame": 1, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q98RA6", "UniRef:UniRef90_D1J7M9" ], "nt": "ATGAAAATATTTGAATTTAAATCAACTGAAATGAGAATTGCAGAGGCAACCCTTAAGAGGATAAATGCTTTAGAACCAGAAATATCAAAATTAAGCAACGAGGAATTGCAACAAAAAACTATTGAGTTTAAGTCGCGTTTAAAAGCAGGAGAAACACCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGTAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGATTTAGGTTCTGTAGCAGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCAACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTAAGTGTTGGGATAAACAAATCTCAAATGTCAAGCGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTGGGTTTTGACTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGTGGGCTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGCGATAATGACGAAAGCAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGCCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTATTTAACAGATAAAGGAATTGAAAAAGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTGCAACAAGCAATTCAAGCAAAAGAACGCGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATTACTTATCAAAATTTTTTCCGTCTATTTAAAAAAATAAGTGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGGGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCACAGCAAATGA", "aa": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSK", "aa_hexdigest": "ae83a3a804cfa4d25aff8f17034ae6fd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9534313725490197, "subject_cov": 0.9328537170263789, "identity": 0.584, "score": 445.0, "evalue": 3.84e-152, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 141517, "stop": 143046, "observations": { "mutation": [ 142741 ], "stop": [ 142741 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M9", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 1.0, "score": 780.0, "evalue": 2.28e-277, "gene_start": 1, "gene_end": 408, "reference_start": 1, "reference_end": 408, "reference_sequence": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSKWKAVTEEVKRVYKKKQPILIGTAQVEDSEILHEYLLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDHWKEIFKEYGEDPIPGEAIRKAFLRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLIEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIETWNKCYDQLRQNIIDKYGISSLINSERNIILSVFDAAWQDHINIMDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK" }, "paralog": false, "identity": 0.9960707269155207, "score": 977.0, "evalue": 0.0, "description": "Internal stop codon at 142741. Nonsense mutation around 142741.", "psc": { "uniref90_id": "UniRef90_D1J7M9", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q98RA6", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M9", "EC:7.4.2.8", "UniRef:UniRef50_Q98RA6", "COG:COG0653", "COG:U", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RA6" ], "product": "Protein translocase subunit SecA" } }, "id": "LMJBGEANGM_287", "locus": "GCA013368015_00253" }, { "type": "cds", "sequence": "contig_1", "start": 142843, "stop": 143163, "strand": "+", "frame": 1, "gene": "secA", "product": "SecA family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "nt": "TTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCAGAAGCAGAAATTATTTCTAAAGCAGGACAAGTAGGAGCAGTTACAATTGCCACAAACATGGCCGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGCGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCAAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGCCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCATTAGATGACCAATTAATTTTGCGTTTTTCAGTTCAAGATCACTGA", "aa": "MLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDH", "aa_hexdigest": "4cd803eb0ed4b784c964843ec9d98f33", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920T749", "query_cov": 0.8113207547169812, "subject_cov": 0.9247311827956989, "identity": 0.698, "score": 119.0, "evalue": 2.96e-33, "valid": false, "product": "SecA family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920T749" }, "pscc": { "uniref50_id": "UniRef50_A0A920T749", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "product": "SecA family profile domain-containing protein" }, "genes": [ "secA" ], "id": "LMJBGEANGM_288", "locus": "GCA013368015_00254" }, { "type": "cds", "sequence": "contig_1", "start": 143227, "stop": 143568, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTTGATGACGTAATTAGACAACAACGTGATTTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTCTTTATAAAAAACAATAGTTTGGATTTAATAGAATTTGTAAAGCATCTAAATAACAATTTTATGATTTTAACAAACAAAGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGTGAAGAATTAATTCAATACTTAATTGAAACCTGA", "aa": "MRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLIEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIET", "aa_hexdigest": "c4260b48df972497f5fd3f9d24e28613", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13543.310099999984, "isoelectric_point": 5.040334129333496 }, "id": "LMJBGEANGM_289", "locus": "GCA013368015_00255" }, { "type": "cds", "sequence": "contig_1", "start": 143695, "stop": 144111, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCATATCAAGTTTACACCCAATTGGGTTCAAAGAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGCACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAACGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAATCAGCAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAACGTGATCATTTGATTAAAGAGGGTTTAGGCGAACAAGAAGTGGATGATAAACTAAAGGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAA", "aa": "MDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK", "aa_hexdigest": "fdd96d929476e5d5db4aad5b81cc4d01", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16172.984399999992, "isoelectric_point": 5.129798698425293 }, "id": "LMJBGEANGM_290", "locus": "GCA013368015_00256" }, { "type": "cds", "sequence": "contig_1", "start": 144119, "stop": 144367, "strand": "-", "frame": 2, "gene": "yjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "db_xrefs": [ "COG:COG1051", "COG:F", "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_D1J7N0", "UniRef:UniRef90_D1J7N0" ], "nt": "TTGCTCAAGGGTTTTAGAGAAGTCAATAAATATATTATTGGAAAATTAATATCTAAAGAAGTTGTTTATTTTATTGCTAGGCCAACATCTTTTGATTTAATTAAGCAAGATAGTGAAATTAAGGTAGTTGAATGGCAAGATACAACAAATGCATTGTTATATCGCATAACATACGAAAGTGATTGTGAAATATTGAAAAAGGCTGTTGAATTTTATTGCGAATATATAACAAAAAATAACGAGTTTTAA", "aa": "MLKGFREVNKYIIGKLISKEVVYFIARPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF", "aa_hexdigest": "e9ce9b7cd46e40429faa44ee87310db2", "start_type": "TTG", "rbs_motif": null, "genes": [ "yjhB" ], "truncated": "3-prime", "pseudogene": { "start": 144119, "stop": 144550, "observations": { "mutation": [ 144460 ], "stop": [ 144460 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N0", "query_cov": 1.0, "subject_cov": 0.5734265734265734, "identity": 0.976, "score": 166.0, "evalue": 1.34e-51, "gene_start": 1, "gene_end": 82, "reference_start": 62, "reference_end": 143, "reference_sequence": "MKKEKSCGAIIFKEENDNLFVLLVEQTAGHWGFPKGHVEKNETEEETAIREVKEETNIDIKLLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF" }, "paralog": false, "identity": 0.972027972027972, "score": 281.0, "evalue": 2.65e-99, "description": "Internal stop codon at 144460. Nonsense mutation around 144460.", "psc": { "uniref90_id": "UniRef90_D1J7N0", "gene": "YjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_D1J7N0", "cog_id": "COG1051", "cog_category": "F", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N0", "EC:3.6.1.17", "UniRef:UniRef50_D1J7N0", "COG:COG1051", "COG:F", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N0" ], "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)" } }, "id": "LMJBGEANGM_291", "locus": "GCA013368015_00257" }, { "type": "cds", "sequence": "contig_1", "start": 144458, "stop": 144550, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGAGAAGTCGTGTGGCGCTATTGTTTTCAAAGAAGAAAATGATAATCTTTTTGTTTTATTAGTTGAACAAACAGCAGGCCATTGA", "aa": "MKKEKSCGAIVFKEENDNLFVLLVEQTAGH", "aa_hexdigest": "202cb7635261bba9771de4438a00fe12", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3378.8710999999994, "isoelectric_point": 5.573540687561034 }, "id": "LMJBGEANGM_292", "locus": "GCA013368015_00258" }, { "type": "cds", "sequence": "contig_1", "start": 144748, "stop": 145140, "strand": "-", "frame": 3, "gene": null, "product": "DUF4199 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5", "UniRef:UniRef90_A0A454C9U5" ], "nt": "ATGGATATTAACAATATTATAAAAAATAATATAAAAAGATTATTAATAACAACAGCGTTTGGCTTTTTAGCGATTTTGATTTCTTTTTTGATTACCGGGATATTTTCAATACAATCGCTTTCAAATAATGGCCGTCTTGCAATATCTTGCTTTGGACAAATAATTTGCGGTTTTTACGTTGTTTATTATATTTTGAATTTCTTTTTAGTATATAGATTTAAATCAGAAATTGCAAACAAAAAGATATTAAAAACTCTATTAATAATTAGCATTTTTATTTTGATATGTGGAATTTGTTTTATTGTTAATGGTTTAATTTTACCTTCAATTAGAAAAACATACATTCAAGCAGTAATGACTAAAAATAATAATTTATTACAAAGTATACTTTGA", "aa": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSNNGRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSIL", "aa_hexdigest": "0da40b1d0fdcb452288f76bf69c7a090", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 144637, "stop": 145140, "observations": { "mutation": [ 144720, 144750 ], "stop": [ 144720, 144750 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U5", "query_cov": 1.0, "subject_cov": 0.7784431137724551, "identity": 0.985, "score": 236.0, "evalue": 3.95e-78, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSSNSRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSILWQGKVIKVEMWISSTIFLVAIASSLILCSFIFKKMNN" }, "paralog": false, "identity": 0.9760479041916168, "score": 309.0, "evalue": 2.76e-109, "description": "Internal stop codon at 144720, 144750. Nonsense mutation around 144720, 144750.", "psc": { "uniref90_id": "UniRef90_A0A454C9U5", "product": "DUF4199 domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U5", "UniRef:UniRef50_A0A454C9U5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5" ], "product": "DUF4199 domain-containing protein" } }, "id": "LMJBGEANGM_293", "locus": "GCA013368015_00259" }, { "type": "cds", "sequence": "contig_1", "start": 145224, "stop": 146108, "strand": "+", "frame": 3, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_D1J7N2", "UniRef:UniRef90_D1J7N2" ], "nt": "GTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGTCCCCACGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTACATAACCAAAGCGATATATTAAAACCATGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCGGCCTTTAACGCTTTATTGAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCTCAAAAAATACCGGAAACTATTTTGAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATTGCTAAATTAGGAAATGGTAGTCTTAGAGATTCATTGAGTATTGCAGACCATGTTGCAATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTACATGTCAATGCATGATATTAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGA", "aa": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNYMSMHDINKTLNLARELFSQGIDINILINDLINILKE", "aa_hexdigest": "88a14fd1da33e862821028650534cf17", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00244E6DAE", "query_cov": 0.8163265306122449, "subject_cov": 0.967741935483871, "identity": 0.629, "score": 304.0, "evalue": 8.38e-101, "valid": false, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A7V0IXX6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "genes": [ "dnaX" ], "truncated": "3-prime", "pseudogene": { "start": 145224, "stop": 146411, "observations": { "mutation": [ 146106 ], "stop": [ 146106 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N2", "query_cov": 1.0, "subject_cov": 0.4407796101949025, "identity": 0.997, "score": 575.0, "evalue": 2.25e-201, "gene_start": 1, "gene_end": 294, "reference_start": 1, "reference_end": 294, "reference_sequence": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKEWIIFNKTRDQSLVSTDFKILLQIKIDDVKAHYYIETLIDQLHSIAISDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQVINKNNIYKSLDLSSIANGFNANNSTIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETADLQISENWKDLVNEDLISKENSENKHVPLETIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKGEILEAKKYWKANINEIRAKKVKPLEDLNLKYNKTVQKDVAWAQEIFGDKFKLNK" }, "paralog": false, "identity": 0.9873417721518988, "score": 769.0, "evalue": 3.71e-280, "description": "Internal stop codon at 146106. Nonsense mutation around 146106.", "psc": { "uniref90_id": "UniRef90_D1J7N2", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_D1J7N2", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N2", "EC:2.7.7.7", "UniRef:UniRef50_D1J7N2", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N2" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "LMJBGEANGM_294", "locus": "GCA013368015_00260" }, { "type": "cds", "sequence": "contig_1", "start": 146166, "stop": 146687, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTAAAATTGATGATGTTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCTATAAGCGATAGTCCAGAAGATTATTTTGAAATTTTGTTATTAAAGTTATGCAACTTTTTAAGCCCAAAAGAAGAATATAAAAATTCTGCAGTTATGCCAAAAATTAGTAATGAGGAATTTAATAATTTTAATAGTCAAGAAAAGCAGGCCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCACAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAAACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGCAATAATCAAATTAGCATAGATTCAAATGTTGATAACCAAGAAACAACCGATTTACAAATATCGGAAAATTGA", "aa": "MQIKIDDVKAHYYIETLIDQLHSIAISDSPEDYFEILLLKLCNFLSPKEEYKNSAVMPKISNEEFNNFNSQEKQAINKNNIYKSLDLSSIANGFNANNSTIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETTDLQISEN", "aa_hexdigest": "075c35e9fc087907e5775ba855a68379", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19596.162099999987, "isoelectric_point": 4.198037147521972 }, "id": "LMJBGEANGM_295", "locus": "GCA013368015_00261" }, { "type": "cds", "sequence": "contig_1", "start": 146799, "stop": 147089, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATGATGGTCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAATATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACGTGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGCTTATGCCATTACAAAAGATGAGATATTAGAAGCAAAGAAATATTGA", "aa": "MLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKDEILEAKKY", "aa_hexdigest": "6e9ad96de4a38e59c3c61f4fab667405", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11182.607599999988, "isoelectric_point": 4.6739500045776365 }, "id": "LMJBGEANGM_296", "locus": "GCA013368015_00262" }, { "type": "cds", "sequence": "contig_1", "start": 147251, "stop": 147550, "strand": "+", "frame": 2, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_012855373.1", "SO:0001217", "UniParc:UPI0001BC20AC", "UniRef:UniRef100_D1J7N3", "UniRef:UniRef50_Q98RF9", "UniRef:UniRef90_D1J7N3" ], "nt": "ATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGACGAACAAGAAATTCAAAAGAAAGAATTTACTATTGAAAAGCAAGGTATAACCGTTGTTATGAATGGTTTGAGAAAATTAGTGTCTATAAAATTTAATCCTGCATTAATCGATCCAGATGATCCTGAATTATTAGAAGATTTATTAATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAA", "aa": "MSMNMNEMLKKARRIQTEMEKDEQEIQKKEFTIEKQGITVVMNGLRKLVSIKFNPALIDPDDPELLEDLLMLAVNEAIETVDKAFDEINAKYSDSGLSF", "aa_hexdigest": "77242d963041dcb031811ba880313b74", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20AC", "ncbi_nrp_id": "WP_012855373.1", "uniref100_id": "UniRef100_D1J7N3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20AC", "RefSeq:WP_012855373.1", "UniRef:UniRef100_D1J7N3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N3", "uniref90_id": "UniRef90_D1J7N3" }, "psc": { "uniref90_id": "UniRef90_D1J7N3", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_Q98RF9", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RF9" ], "product": "Nucleoid-associated protein MYPU_0500" }, "genes": [ "ybaB" ], "id": "LMJBGEANGM_297", "locus": "GCA013368015_00263" }, { "type": "cds", "sequence": "contig_1", "start": 147550, "stop": 148140, "strand": "+", "frame": 1, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "COG:COG0353", "COG:L", "RefSeq:WP_012855374.1", "SO:0001217", "UniParc:UPI0001BC20AD", "UniRef:UniRef100_D1J7N4", "UniRef:UniRef50_D1J7N4", "UniRef:UniRef90_D1J7N4" ], "nt": "ATGAACAATATTGAGATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAAGTGAAGATATAGATCAAATTTGCAATCAAATAAAAGCATTGAGAAATTCAATAAAGAAGTGTAATATTTGCAATAGATATAACGAAAATACAATTTGCAATATTTGTTTGGACACTAATCGAACAAAACAACTTATGTTAGTTGGCAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAGCTTAAAGAATGAAGATTTTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGTCAATTTAATATGCATTATATTAAAAAATTCTTGAAAAACAAAATAAATTTTACAAATGTTTTTCAATTGTCAACTGGTATTCCTTTGGGCTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATCAAAATGTAG", "aa": "MNNIEIQKLEELLKTVFDFTKKQSQKFIFNIFKSSSEDIDQICNQIKALRNSIKKCNICNRYNENTICNICLDTNRTKQLMLVGNSLDIDKFENIGLYKGKYFVFDQNISLKNEDFIVSSLLEKLIDILKDKNELILSLSPTIEGQFNMHYIKKFLKNKINFTNVFQLSTGIPLGSSVEYIDQDTLKESLLNKIKM", "aa_hexdigest": "dd189323ff9c73c9ac6682edf62eaf5a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC20AD", "ncbi_nrp_id": "WP_012855374.1", "uniref100_id": "UniRef100_D1J7N4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20AD", "RefSeq:WP_012855374.1", "UniRef:UniRef100_D1J7N4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N4", "uniref90_id": "UniRef90_D1J7N4" }, "psc": { "uniref90_id": "UniRef90_D1J7N4", "gene": "RecR", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_D1J7N4", "cog_id": "COG0353", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N4" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "LMJBGEANGM_298", "locus": "GCA013368015_00264" }, { "type": "cds", "sequence": "contig_1", "start": 148155, "stop": 148460, "strand": "+", "frame": 3, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_A0A448ZVP3", "UniRef:UniRef90_D1J7N5" ], "nt": "ATGTCAAAGGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAAATTAAAAGAATATTTAGAGTCTAAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCCTTATTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGTGTTTGGCCAGGATTAGCAAAAAATAAAACGGTTATTTCAGATAGGTATTGA", "aa": "MSKGKFVVFEGMDGSGKTTIIKKLKEYLESKNRLDDFVFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLAKNKTVISDRY", "aa_hexdigest": "6ff31c7bf4ecac29176dde88d85ec1e0", "start_type": "ATG", "rbs_motif": "AGGAGG", "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 148155, "stop": 148763, "observations": { "mutation": [ 148458, 148683 ], "stop": [ 148458, 148683 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N5", "query_cov": 1.0, "subject_cov": 0.47417840375586856, "identity": 0.98, "score": 197.0, "evalue": 1.27e-62, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MSKGKFVVFEGMDGSGKTTIIKKLKEYLESKNRLDDFIFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLEKNKTVISDRYWTSSFVYQGVLGGLGLKKVKDINNIATEETTPDLTIFFDMEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSYWYVINQGNEKYEIIKSDCNIDELFKRVLNVLKENKII" }, "paralog": false, "identity": 0.9801980198019802, "score": 392.0, "evalue": 1.35e-141, "description": "Internal stop codon at 148458, 148683. Nonsense mutation around 148458, 148683.", "psc": { "uniref90_id": "UniRef90_D1J7N5", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_A0A448ZVP3", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N5", "EC:2.7.4.9", "UniRef:UniRef50_A0A448ZVP3", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVP3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVP3" ], "product": "Thymidylate kinase" } }, "id": "LMJBGEANGM_299", "locus": "GCA013368015_00265" }, { "type": "cds", "sequence": "contig_1", "start": 148575, "stop": 148685, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGA", "aa": "MEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSY", "aa_hexdigest": "a43bed897f7f5d7fb1812dc0843d9f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4420.9522000000015, "isoelectric_point": 8.064688682556149 }, "id": "LMJBGEANGM_300", "locus": "GCA013368015_00266" }, { "type": "cds", "sequence": "contig_1", "start": 148834, "stop": 149667, "strand": "+", "frame": 1, "gene": "dnaX", "product": "DNA polymerase III subunit", "db_xrefs": [ "COG:COG2812", "COG:L", "RefSeq:WP_175936667.1", "SO:0001217", "UniParc:UPI00158EF18D", "UniRef:UniRef100_UPI00158EF18D", "UniRef:UniRef50_D1J7N6", "UniRef:UniRef90_D1J7N6" ], "nt": "ATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAACAATAAGCTCCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTGTTAGATTAGTTAATGATGAAAAACAAATAAGTTGGCATGAATGTTTGCAAAAAGAATATTGTTTTATTATTGATGGAAGCATAGATGAAATATCGAAAGAAAATTTACAGGAATTTTTAAATCGTGTTTCCAAAAGCTCTCTATTTGCTAATAAGTATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTGAAATTTTTAGAAAATCCTCCATTTAATACAATAATATTATTACATACAATAAAGCCTCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAGTTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATATTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTATGAATTAAAAAATAGTATAAAAAAGGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAAGTTAATTTTAAAATTAAAGGAAGAATATGAATAA", "aa": "MINKNLSKVLENSIKNNKLQQVYLLFSRANQDIDLDILEFVRLVNDEKQISWHECLQKEYCFIIDGSIDEISKENLQEFLNRVSKSSLFANKYKFLIIKCVENITLKAANSILKFLENPPFNTIILLHTIKPQNVLKTIKSRCFIIDIRDDFNAVASVESFFITYKIDVSKQKQFRELADLIYNSPANLFNLILFYIQQLNIENYKNIIHFSIFIFKQIYELKNSIKKDKNISKFDSISILNIINNLYNFVELIDNNNCIFNIQKAKLILKLKEEYE", "aa_hexdigest": "f219062ab23e064da99b4ad56846c349", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158EF18D", "ncbi_nrp_id": "WP_175936667.1", "uniref100_id": "UniRef100_UPI00158EF18D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EF18D", "RefSeq:WP_175936667.1", "UniRef:UniRef100_UPI00158EF18D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EF18D", "uniref90_id": "UniRef90_D1J7N6" }, "psc": { "uniref90_id": "UniRef90_D1J7N6", "gene": "DnaX", "product": "DNA polymerase III subunit", "uniref50_id": "UniRef50_D1J7N6", "cog_id": "COG2812", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N6" ], "product": "DNA polymerase III subunit" }, "genes": [ "dnaX" ], "id": "LMJBGEANGM_301", "locus": "GCA013368015_00267" }, { "type": "cds", "sequence": "contig_1", "start": 149660, "stop": 150373, "strand": "+", "frame": 2, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0008649", "GO:0008757", "KEGG:K07056", "RefSeq:WP_113248106.1", "SO:0001217", "UniParc:UPI000DDF25A5", "UniRef:UniRef100_UPI000DDF25A5", "UniRef:UniRef50_D1J7N7", "UniRef:UniRef90_D1J7N7" ], "nt": "ATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTACGAAATTAATAACAAGCGCCTAATTGCATACCATAAATTTAATGAAAAAGAAATGACACCTAAAATTATTGAATTATTGCAACAGGGGATGATTATTTCATTGATTTCTGATGCCGGCATGCCTTGTGTTTCTGATCCTGGTTTTGAAGTTATTAAAGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGCGGCCCAACTGCTCTAATACATGCAATAATAAAGGCTAATTTAGACAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCAGGTGCAAGACAAAATCAATTAAAATCCTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAATGCAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAA", "aa": "MNNKIYIVGTPIGNLEDITYRAIKVLQSSDIILCEDIRVSQKLLQHYEINNKRLIAYHKFNEKEMTPKIIELLQQGMIISLISDAGMPCVSDPGFEVIKAAKAANIGIDVIGGPTALIHAIIKANLDNCFTFLGFLKDKSGARQNQLKSLTFGTYVAYVSPHKLLSTIKDFEIIFQEQVKLFLIKEMTKMFETSFEGTPQEVLMQLSEEDQRGEYTLVFSFKEPKKLKINKYKKEIK", "aa_hexdigest": "fd9899736cb15d8716c707070ed7c70b", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000DDF25A5", "ncbi_nrp_id": "WP_113248106.1", "uniref100_id": "UniRef100_UPI000DDF25A5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DDF25A5", "RefSeq:WP_113248106.1", "UniRef:UniRef100_UPI000DDF25A5" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DDF25A5", "uniref90_id": "UniRef90_D1J7N7" }, "psc": { "uniref90_id": "UniRef90_D1J7N7", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "LMJBGEANGM_302", "locus": "GCA013368015_00268" }, { "type": "cds", "sequence": "contig_1", "start": 150417, "stop": 150680, "strand": "-", "frame": 1, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02968", "RefSeq:WP_012855378.1", "SO:0001217", "UniParc:UPI0001BC20B1", "UniRef:UniRef100_D1J7N8", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_D1J7N8" ], "nt": "ATGGCAAACATTAAATCAAAACAAAAAGCAATATTATCTAACGAAAAAGCAAACATCCGTAATTCAGCCGTTAAATCATCAGTTAAAACAGCTATTAAGAAAGCAAAAATTGCTGCTGTTAATAAAGATCCTAAAGCTGCTGAATTTCAAGCTAATGCTCACCATGAAATTGACAAAGCTGTTTCAAAGGGCGTTTTACATCAAAACAATGGTTCTAGAAAAGCTAGCCGTTTAGATGCATTTATTGCTAAAAATAACAACTAA", "aa": "MANIKSKQKAILSNEKANIRNSAVKSSVKTAIKKAKIAAVNKDPKAAEFQANAHHEIDKAVSKGVLHQNNGSRKASRLDAFIAKNNN", "aa_hexdigest": "19721fc9eea4c2bbc137fa79a30a1504", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC20B1", "ncbi_nrp_id": "WP_012855378.1", "uniref100_id": "UniRef100_D1J7N8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20B1", "RefSeq:WP_012855378.1", "UniRef:UniRef100_D1J7N8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N8", "uniref90_id": "UniRef90_D1J7N8" }, "psc": { "uniref90_id": "UniRef90_D1J7N8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "LMJBGEANGM_303", "locus": "GCA013368015_00269" }, { "type": "cds", "sequence": "contig_1", "start": 150774, "stop": 151049, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTGAACAAGGAACAAGAAATCGTAGCCTATGTGCCACGTAAGTATATGAATACAATAATACTGGATAATTTTTTAAAATCTTCGGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAAAATAAATTTTATTTATGA", "aa": "MVTKKKYDGYLKYSKNNESELINYYLTNNKNIYLKLNKEQEIVAYVPRKYMNTIILDNFLKSSVPKLLEIKTKIEQKKILAIDIEKNKFYL", "aa_hexdigest": "56f9b88474e4b9eb27b636cdc7c7c8a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10943.76899999999, "isoelectric_point": 9.566286277770995 }, "id": "LMJBGEANGM_304", "locus": "GCA013368015_00270" }, { "type": "cds", "sequence": "contig_1", "start": 151491, "stop": 151832, "strand": "+", "frame": 3, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0", "UniRef:UniRef90_D1J7P0" ], "nt": "ATGGAAAATAAAACAAAATTTATAAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCTATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAAGGATTTTTAGATTCATACAATGCACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCCAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTTTTATATTAAAGCCTTATATGTATGACTCCGACTCTTCTATTCAAGGTGACTATTTTAGCAACGAATGA", "aa": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDSYNAQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFILKPYMYDSDSSIQGDYFSNE", "aa_hexdigest": "2333d06072b51362c5ef26fbe6a6d354", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 151491, "stop": 152129, "observations": { "mutation": [ 151830 ], "stop": [ 151830 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P0", "query_cov": 1.0, "subject_cov": 0.5330188679245284, "identity": 0.991, "score": 197.0, "evalue": 2.02e-62, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDSYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFILKPYMYDSDSSIQGDYFSNEWILTKSNGKKSKIPNQLISLKKSMNNFGLLFSNEMSICSIFVHNLSTELNIEDLPSFQGIYNVKDLCASIKSVYNSNINSMYNQSVSTIFNVLNNKIAK" }, "paralog": false, "identity": 0.9811320754716981, "score": 362.0, "evalue": 7.56e-130, "description": "Internal stop codon at 151830. Nonsense mutation around 151830.", "psc": { "uniref90_id": "UniRef90_D1J7P0", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P0", "UniRef:UniRef50_D1J7P0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0" ], "product": "NERD domain-containing protein" } }, "id": "LMJBGEANGM_305", "locus": "GCA013368015_00271" }, { "type": "cds", "sequence": "contig_1", "start": 151899, "stop": 152129, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTACATAATTTAAGCACGGAACTAAATATAGAAGATTTGCCATCGTTTCAAGGAATTTGCAATGTTAAAGATTTGTGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTGTTTCAACTATTTTCAATGTTTTAAATAATAAAATTGCAAAATAA", "aa": "MNNFGLLFSNEMSICSIFVHNLSTELNIEDLPSFQGICNVKDLCASIKSVYNSNINSMYNQSVSTIFNVLNNKIAK", "aa_hexdigest": "0e9bf6c979980a3b50b64596d209f8ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8496.635599999994, "isoelectric_point": 5.537618446350096 }, "id": "LMJBGEANGM_306", "locus": "GCA013368015_00272" }, { "type": "cds", "sequence": "contig_1", "start": 152289, "stop": 152477, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGAACAAAAAACAGTATTGGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGA", "aa": "MAKEQKTVLEKYYISEAFDKDRNISFNFKKANKGISGNFLNFSSAVEEFIKVSENTQNPTKC", "aa_hexdigest": "c6147096031e44b0c32b36152fe9cf71", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7128.958700000002, "isoelectric_point": 8.787637138366701 }, "id": "LMJBGEANGM_307", "locus": "GCA013368015_00273" }, { "type": "cds", "sequence": "contig_1", "start": 152495, "stop": 152830, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGTTAGAAGCGATAAACACACTGTTAGACCAATAAATAGTTTTTCTCTGCCTTTGTGGTCTTGTATACCTGAACAAACAATGTTATTTGAAGATTTCTTTGTTCAATATTCTTTTCAATGTTTGTTGTTTTCAATACCGGTTAAACAATCACAAGAGCATGAGCATTCACATTCTTGTTTTGGTTCTTCAACTTTCTTAGCTTTTGCTGCAGGTTTTTCAACCAATTGTTCTCTTTCGATGAATTCAATAGCTTCTTCATTTTTAACTTGTTTTTCAGCAACTTTTTCAACAATAACACGGGCACCATCAATGTTTACGCATCCGCGGTAA", "aa": "MIVRSDKHTVRPINSFSLPLWSCIPEQTMLFEDFFVQYSFQCLLFSIPVKQSQEHEHSHSCFGSSTFLAFAAGFSTNCSLSMNSIASSFLTCFSATFSTITRAPSMFTHPR", "aa_hexdigest": "2ccc486cc0d096696d491e9e8e4d19ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12520.17129999998, "isoelectric_point": 6.939211082458496 }, "id": "LMJBGEANGM_308", "locus": "GCA013368015_00274" }, { "type": "cds", "sequence": "contig_1", "start": 152924, "stop": 153163, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "TTGTATAACGAATTTTTAGATGAAGATTTTAATGTTAATATTGATATAAGACCAGAAAGAATAAATAAAAAGATTCGTGATGCTCAAATTTTAAAGACCAAATTTATAGTAGTAATTGGAAAAAAAGAAATTGAAACAAATACCTTATCAGTTAGAGAATATGGAAAAGATGATTCTAAAACATACAGCAAAGAAGAATTTGTAAATTACTTAAAACAATTAAAAAAGAATTTAAAATAA", "aa": "MYNEFLDEDFNVNIDIRPERINKKIRDAQILKTKFIVVIGKKEIETNTLSVREYGKDDSKTYSKEEFVNYLKQLKKNLK", "aa_hexdigest": "99f32b09a620bc38621e8ebdbe80c80c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 72, "aa_cov": 0.9113924050632911, "hmm_cov": 1.0, "evalue": 1.9060067215122512e-18, "score": 61.9974250793457, "start": 2, "stop": 73 } ], "seq_stats": { "molecular_weight": 9487.799599999995, "isoelectric_point": 9.125515937805176 }, "id": "LMJBGEANGM_309", "locus": "GCA013368015_00275" }, { "type": "cds", "sequence": "contig_1", "start": 153227, "stop": 153514, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGCGTGAAGTTTTAGGTGAATTAAATATTAAATATAAAGAATTTATTGGAGAAGCTGCATTTTATGGACCTAAGGTTGATTTTCAAGTAAAAACTGCACTAAATAGAATTATTACTATGTCAACCTTACAATTAGACTTCTTATTGCCATCAAGATTTGAAATGAAATTTGTTGATAGTAATGAACAATTTGTAACACCAGTATTAATTCATAGAGGTTTAATTGGAACTTATGAAAGATTTATTGCAACACTACTTGAACAAACAAAAGGTGTATTGCCATTTTGA", "aa": "MREVLGELNIKYKEFIGEAAFYGPKVDFQVKTALNRIITMSTLQLDFLLPSRFEMKFVDSNEQFVTPVLIHRGLIGTYERFIATLLEQTKGVLPF", "aa_hexdigest": "f194bf682ba591db6ef0c59e7468d337", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8736842105263158, "subject_cov": 0.8217821782178217, "identity": 0.578, "score": 102.0, "evalue": 1.71e-26, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 78, "aa_cov": 0.7894736842105263, "hmm_cov": 0.9743589743589743, "evalue": 1.1089239846117922e-14, "score": 50.41576385498047, "start": 14, "stop": 88 } ], "seq_stats": { "molecular_weight": 10973.738299999988, "isoelectric_point": 6.570439720153809 }, "id": "LMJBGEANGM_310", "locus": "GCA013368015_00276" }, { "type": "cds", "sequence": "contig_1", "start": 153533, "stop": 153901, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTTTGCCCCAATTAAAATGGATAACGAAACATTAGTTGCTAGACCTATGACCTGCCCTCACCATATAATATTATTCAATTCAACAAGACGTTCATACAAAGACTTGCCAATTAGATATTCAGAACAATCAAGGTTATATCGTTACGAAGCATCGGGCGCTTTAAGTGGACTTGAAAGAGTTAGGGGAATGGATCTAACAGAAGGTCACATTTTTGTTAGACCAGATCAAATAAAATCAGAATTTAAACACTTGTATAAAATGATATTGCAAGCATTAAAAGATTTTAATATTGAAATAGATCATATTTCTTTATCATTAAGAGATCCACAAGATACTCAAAAATTCTTCCAAAATGATGAAATGTGA", "aa": "MFAPIKMDNETLVARPMTCPHHIILFNSTRRSYKDLPIRYSEQSRLYRYEASGALSGLERVRGMDLTEGHIFVRPDQIKSEFKHLYKMILQALKDFNIEIDHISLSLRDPQDTQKFFQNDEM", "aa_hexdigest": "97320dfde981601fae09eb5d9d7d2548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9807692307692307, "evalue": 7.314963514356601e-22, "score": 73.80873107910156, "start": 8, "stop": 109 } ], "seq_stats": { "molecular_weight": 14435.421899999981, "isoelectric_point": 6.96768741607666 }, "id": "LMJBGEANGM_311", "locus": "GCA013368015_00277" }, { "type": "cds", "sequence": "contig_1", "start": 153920, "stop": 154039, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTCATAATGCAATTAGAGATTATGTTTTAAAACTTGATAAAAAATTTGGTTTTAGAGAAGTACTAACTCCACACTTTGGTGAAAAGAAGCTTTATGAAATAAGTGGACACTGA", "aa": "MKIHNAIRDYVLKLDKKFGFREVLTPHFGEKKLYEISGH", "aa_hexdigest": "a17315a2b28feeeb87eb514c8fb89092", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4659.415, "isoelectric_point": 9.5220609664917 }, "id": "LMJBGEANGM_312", "locus": "GCA013368015_00278" }, { "type": "cds", "sequence": "contig_1", "start": 154052, "stop": 154213, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAAGAATCTATGGTACTTGTTGGGAAACAAAAGATGAATTAGCAAAATATTTAGATATCTTAAGGGAAAGAAAAGAAAGAGATCATCGTAAAATAGGAAGAGATTTAAATTTATTCGCTTTTAACCAATTAGGTGGACAAGGATTTCCATTTTGA", "aa": "MLTRIYGTCWETKDELAKYLDILRERKERDHRKIGRDLNLFAFNQLGGQGFPF", "aa_hexdigest": "478b3071f748e5e401dad020d09cede0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6361.2328, "isoelectric_point": 8.976658439636232 }, "id": "LMJBGEANGM_313", "locus": "GCA013368015_00279" }, { "type": "cds", "sequence": "contig_1", "start": 154346, "stop": 154663, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCAAATAAAAAATTAAATCATTCAGCAAGCCATTTATTAGCGGCTGCTATATTAAAACTTTATCCAAATGCTAAATTGGCAATTGGACCAGCCATTGAAGAAGGGTTCTATTACGACTTTGAATTTGAAAATCCAATTTTAGAATCAGATCTTCCAAATATTGAAAAAGTAATGAAAGAATTAGCAAAAGAAGGTTATGAAATGCGAAAAGTTGGCATTGAACAATATTCTTTTGAAAATCAACCATATAAAAAAGAACTTTATGAAGAATTTAAAAAAGAAGGAAAAGAAATAACATTCTTTCAATATTGA", "aa": "MEANKKLNHSASHLLAAAILKLYPNAKLAIGPAIEEGFYYDFEFENPILESDLPNIEKVMKELAKEGYEMRKVGIEQYSFENQPYKKELYEEFKKEGKEITFFQY", "aa_hexdigest": "5b3a71cd39b162d39bb0cc2e71f1f051", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12319.913599999993, "isoelectric_point": 5.108199882507324 }, "id": "LMJBGEANGM_314", "locus": "GCA013368015_00280" }, { "type": "cds", "sequence": "contig_1", "start": 154663, "stop": 155337, "strand": "-", "frame": 3, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACGCAATTTAAAGATAAATCAGCTAAAATAAAAACTGCAAACGGTATGGAAACAATTCCAACAGGAATTTTGATGTATCCTACCTTAATGGTTGGCGATATATTACTATATAATGCAGATATTATTCCCGTTGGTATTGACCAAAAGCAACATGTTGAATTGACTAGAAATTTAGCAATTAGATTGAATAATAAATTCAATACAACGTACAAAATACCTGAACCATATATACCAGAAGTTGGGGCCAAAATAATGTCTTTAACTGACCCAAGTAAGAAAATGTCAAAATCTGATACAAATCCTAAATCAGCAATTTATTTATTAGATGATCCAGAAGATGCATACAAAAAAATATTAAAAGCTGTTACAGATTCAGAAGGAAAAATATACTTGTCAGATGAAAAGCCGGGCATAAAAAACCTTTTGACTATATATAGTGCTTTAAATGATATTTCATTACAAGAAAGCGCTGAAAAATTTAAAAACAGCAATTACGGTGAATTTAAAAATGCTGTCGCTCAATGTGTAAAGGAATTTTTAATTAAGATCCAAGATAAGTATAAAAATGCCTTAAATCAAATTGAAACATTGAGTAAAGAGGGAGCAAAAAAGGCAAGTGAAGTTGCCCAAATTAACTTAGATATGTTATTAAAAGGAATAGGATTAAGATAA", "aa": "MTQFKDKSAKIKTANGMETIPTGILMYPTLMVGDILLYNADIIPVGIDQKQHVELTRNLAIRLNNKFNTTYKIPEPYIPEVGAKIMSLTDPSKKMSKSDTNPKSAIYLLDDPEDAYKKILKAVTDSEGKIYLSDEKPGIKNLLTIYSALNDISLQESAEKFKNSNYGEFKNAVAQCVKEFLIKIQDKYKNALNQIETLSKEGAKKASEVAQINLDMLLKGIGLR", "aa_hexdigest": "fd7b8c8172c37627a113216ad05a7c0b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 1.0, "subject_cov": 0.9333333333333333, "identity": 0.71, "score": 323.0, "evalue": 1.59e-109, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "LMJBGEANGM_315", "locus": "GCA013368015_00281" }, { "type": "cds", "sequence": "contig_1", "start": 155377, "stop": 155664, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATTCTGAAAAGAAGACGTTATTAAGCGGAATTACTGCAACTGGTAAATTAACGCTAGCAAATTATATTGGCGCTATAAAAAATATGGTTAAATTGCAAGAAGAATATAACAATTACATTTTTATAGCAGATTTACATGCTCTAACGTTGCCAATTGATCCTAAGCAATTATCAAAAAATAAAAAGGATATTTTTGCACTTTATATTGCATGTGGAATTGACCCTAAAAAGAGTGTGTTATTTTTTCAATCAGATGTCATTCAACATGGATTAATGAATTGA", "aa": "MQNSEKKTLLSGITATGKLTLANYIGAIKNMVKLQEEYNNYIFIADLHALTLPIDPKQLSKNKKDIFALYIACGIDPKKSVLFFQSDVIQHGLMN", "aa_hexdigest": "a613fab4aa3aa15dabfc15552bb97150", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10644.364899999991, "isoelectric_point": 9.041578102111817 }, "id": "LMJBGEANGM_316", "locus": "GCA013368015_00282" }, { "type": "cds", "sequence": "contig_1", "start": 155983, "stop": 156219, "strand": "+", "frame": 1, "gene": "ptsG2", "product": "PTS EIIB type-1 domain-containing protein", "db_xrefs": [ "COG:COG1264", "COG:G", "SO:0001217", "UniRef:UniRef50_N9UZL6", "UniRef:UniRef90_D1J7P4" ], "nt": "ATGCAAGATTTAATTTTGTATTTGGGTGGAATTTCAAATATAGTTTCTTCATCAGCAACTCAAAAAATTGTAAGAATTTCATTTAAAAATAAAGCAAATATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCAGGTATTGCATTTCAATCTTCAAATATATCATTAGTTGTTGGAAATTGCGCAAATTATTTATCTACATTACTTAATAAGGAATTAAACAATCATGAATAA", "aa": "MQDLILYLGGISNIVSSSATQKIVRISFKNKANISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE", "aa_hexdigest": "bc1f12fee655ac27acbd44e239323904", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG2" ], "truncated": "5-prime", "pseudogene": { "start": 155808, "stop": 156219, "observations": { "insertion": [ 155841 ], "stop": [ 155914 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P4", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.987, "score": 146.0, "evalue": 7.59e-44, "gene_start": 1, "gene_end": 78, "reference_start": 59, "reference_end": 136, "reference_sequence": "ISYIGKTIIGEKMLNKNKFLYIFLIIITLGLVLIFWRKKYKILKTKNYLTTDNEITFSMQDLILYLGGISNIVSSSATQKIVRISFKNKTNISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE" }, "paralog": false, "identity": 0.9632352941176471, "score": 248.0, "evalue": 1.49e-86, "description": "Internal stop codon at 155914. Frameshift due to insertion around 155841.", "psc": { "uniref90_id": "UniRef90_D1J7P4", "gene": "PtsG2", "product": "PTS EIIB type-1 domain-containing protein", "uniref50_id": "UniRef50_N9UZL6", "cog_id": "COG1264", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P4", "UniRef:UniRef50_N9UZL6", "COG:COG1264", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_N9UZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZL6" ], "product": "PTS EIIB type-1 domain-containing protein" } }, "id": "LMJBGEANGM_317", "locus": "GCA013368015_00283" }, { "type": "cds", "sequence": "contig_1", "start": 156212, "stop": 156586, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0", "UniRef:UniRef90_UPI0024114FF0" ], "nt": "ATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTATAACAATTTTGGCCAAGAAATATTTGATCAAATTTTCAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAAGAAATAATAACGATTTATAAAGATAATAATGTCAGAATCGAACAATCAATATTTAAGATGCTATCAACTGATTTTGAAAAAGCTAAAACCTTATTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGA", "aa": "MNNKDSKYQTLMANLLNIDTYDTSLYTSINSENFFDIYNNFGQEIFDQIFKNYKFNCILTEVKNKEMRQLLEKAQNKQEIITIYKDNNVRIEQSIFKMLSTDFEKAKTLLITKLESEFQKSIVN", "aa_hexdigest": "11dc00f925aeb645eb72db61d3602459", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024114FF0", "query_cov": 1.0, "subject_cov": 0.8157894736842105, "identity": 1.0, "score": 153.0, "evalue": 1.46e-45, "valid": true, "uniref50_id": "UniRef50_UPI0024114FF0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024114FF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14779.667499999985, "isoelectric_point": 5.516190147399901 }, "id": "LMJBGEANGM_318", "locus": "GCA013368015_00284" }, { "type": "cds", "sequence": "contig_1", "start": 156641, "stop": 156889, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCTTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCCTTGGTTTATTTAAAATCGAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGCTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATCGAATCTATATTTGAGCATTTAAAAAAAATTTGA", "aa": "MHVGFLFISIKTDKKTIFAPLLFKEVTLEVKNSLVYLKSNSDVRINSKLVTFLSQEGFLLNIDNLDFSNLSIESIFEHLKKI", "aa_hexdigest": "3f3eadc5954fe1b6a13a61b8c2de92a7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9433.956899999994, "isoelectric_point": 8.027168083190919 }, "id": "LMJBGEANGM_319", "locus": "GCA013368015_00285" }, { "type": "cds", "sequence": "contig_1", "start": 156905, "stop": 157027, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCTGGATATTTATGA", "aa": "MPQSLRSNIPLVSQSDIQNVSIEFHPGMIFGFYNVSSGYL", "aa_hexdigest": "3b3542bb815f6037943a18df730e216e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.0337, "isoelectric_point": 5.3026460647583 }, "id": "LMJBGEANGM_320", "locus": "GCA013368015_00286" }, { "type": "cds", "sequence": "contig_1", "start": 157034, "stop": 157222, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATCAACACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCTACAGTGTCTGCACTATGTCAAGATACAATAATTTGA", "aa": "MKKIIENDEFESILVPEINKNVYREKINTSIFNNKFKLFKVQKTNFSQDVATVSALCQDTII", "aa_hexdigest": "82aa51f4507ea3369e6fa1a6adb94bd3", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.282900000003, "isoelectric_point": 7.844142341613771 }, "id": "LMJBGEANGM_321", "locus": "GCA013368015_00287" }, { "type": "cds", "sequence": "contig_1", "start": 157304, "stop": 158122, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAGAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAAAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAGGGCTATGTCGATACATTGAATGAAATAGAAGCACTGCCAAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATTTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATACGAAGTAAATTTAAAAAGAAGACCGTCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATAAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTATTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCGTTTGCAATGTCTATAAGAACAGGTAGCTTAAAACCATATATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGA", "aa": "MSQKKAALDVLRNRLGQLSLFCLFALNDKNLRQEVFYEPLKKFIEKIENFSISQVEKGIQIFSDEDKGYVDTLNEIEALPNLNNLLHFYASTLNANLTINDFNTLRLLAKDIKYDVEGHTFNDKKELKKYLYEVNLKRRPSIFSIYPRSIKEASELIINAPELFKINVDEAVKYIDKVEFAELETFVEKYKNLLIKKTINVSNSAVLSNMLFAKLINTISNFNDEQKREYTSFAMSIRTGSLKPYMFFHRYKNIIKVLFPIIVTTPELDLSM", "aa_hexdigest": "87c00da7256fcdfefac955d39ce4ccd3", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31726.381600000037, "isoelectric_point": 8.651479911804202 }, "id": "LMJBGEANGM_322", "locus": "GCA013368015_00288" }, { "type": "cds", "sequence": "contig_1", "start": 158294, "stop": 158503, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATATTGAATCTCTTTTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTAGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAGACCAATTGATGTAATTCAAGTTGATGGCGAATGA", "aa": "MGDIESLLDYATARGTYSILLDKNYRSKRASLMTFSSKHFYDSKLDVIDDFKLAFSSERPIDVIQVDGE", "aa_hexdigest": "88f9d4fb6c1a4b670a1b4e6bd3b74ede", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7884.7716, "isoelectric_point": 4.954848289489745 }, "id": "LMJBGEANGM_323", "locus": "GCA013368015_00289" }, { "type": "cds", "sequence": "contig_1", "start": 158618, "stop": 159301, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "TTGACAAATGCAATATTTAGCGTTGAACCAGAATTGGAAAAAGCCTTATTTTCTGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGACGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGTGGGAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTCAAAGAATGGCTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCAAAATTAAACGAATTATTGAAAGAACTAAATGCTGACAATTTCGAAATTATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGACGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGA", "aa": "MTNAIFSVEPELEKALFSDKITIKNIENIQGDEADLVIMSVVYDKKTALFGTYVARKGGKNALNVAISRAREKIIVVKSIYADDVEINERSTSDMRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMDVLAKLNELLKELNADNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRIS", "aa_hexdigest": "85cc79b111d1911a76bd9d1cf6eac25c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 64, "aa_cov": 0.2775330396475771, "hmm_cov": 0.9375, "evalue": 6.082652545849997e-12, "score": 41.0362548828125, "start": 15, "stop": 77 } ], "seq_stats": { "molecular_weight": 26226.084300000042, "isoelectric_point": 5.757699012756347 }, "id": "LMJBGEANGM_324", "locus": "GCA013368015_00290" }, { "type": "cds", "sequence": "contig_1", "start": 159380, "stop": 160429, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07", "UniRef:UniRef90_UPI00215D9F07" ], "nt": "TTGTCTGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTTCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTAATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAGAGCCGAATACTCAAAGGACTATGAAAAAGATTTTTTGATTAATTATAATGATGTTGAATTGGATAAAACGCATATTGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGTCATAAAGATTGAAGATGTCACTTCATCATCGGCTAACGATGAATTAAAAGTAACTAATTCTAATGAACAAAAAATTTCTGCAAAAACAGCAAGTGAAGATATTGAATTGGGATTTTTAGTTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACGTTCGATGATCTTGATATAAATATATCACCTGAAAGCGAACAACAAGTTGAAGCTAAACTCAATTTGGATTCAGGCATTATTCAAGATAAGTTGCATGAGCAAGACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACAAAAGAATGATGATGTTCAAAGTCCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAACGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGATGAAAACGAAGAAACTTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATACATGAAAAATAAAGATATTGAACAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTCAACGTGGAGGACAACAATGTCAAAAAATAG", "aa": "MSEENNNLNVEEIKENIIENNALKNIKSSQIKQGSELKEIDFENVDDETFEVNQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLINYNDVELDKTHIDQEFHIENEFESTINNSNVIKIEDVTSSSANDELKVTNSNEQKISAKTASEDIELGFLVEDINKENISNLFSNTFDDLDINISPESEQQVEAKLNLDSGIIQDKLHEQDDEYFNLEENNIEEQQKNDDVQSPDETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK", "aa_hexdigest": "d0fe2af050f7850ff4048210bab65dc4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 159080, "stop": 160429, "observations": { "mutation": [ 159299 ], "stop": [ 159299 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9F07", "query_cov": 1.0, "subject_cov": 0.6856581532416502, "identity": 0.934, "score": 603.0, "evalue": 3.68e-214, "gene_start": 1, "gene_end": 349, "reference_start": 161, "reference_end": 509, "reference_sequence": "MRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMAVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRISWIVENKQILNKIKILIKHEEELNSQILLSEENNNLNVEEIKENIIENNAIKNIKSNQIKQSPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLINYNDIELDNTHIDQEFHIENEFESTINNSNAVKIEDATSSSANDELKITNSNEQKISAKTASEDIESGFSIEDINKENISNLFSNTFDDLDINMALESEQQVEAKLNYDSGIVQDKLHEQNDEYFNLEENDIEEQQKNNDVQSPDETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK" }, "paralog": false, "identity": 0.9465478841870824, "score": 846.0, "evalue": 3.69e-312, "description": "Internal stop codon at 159299. Nonsense mutation around 159299.", "psc": { "uniref90_id": "UniRef90_UPI00215D9F07", "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9F07", "UniRef:UniRef50_UPI00215D9F07" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 40696.01110000005, "isoelectric_point": 4.096465873718262 }, "id": "LMJBGEANGM_325", "locus": "GCA013368015_00291" }, { "type": "cds", "sequence": "contig_1", "start": 160491, "stop": 160742, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAGGATATTGGAACTAAAATCGCAAAGCAAAAAAAGATTAAAAAAATTTTATTCATAACTCTTTCTGTTGCTGGTATCGTTACAATCGTAGGGGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTATATTGATGTTTCTCTATATTCACAAATAAATTCTAAATTTAAAGAATTGTATCTCGATGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGA", "aa": "MHKDIGTKIAKQKKIKKILFITLSVAGIVTIVGATIGVTSRKKMELVTYDDYIDVSLYSQINSKFKELYLDGKQPDKKNYPIT", "aa_hexdigest": "8086147ac78e9f3064dce15391cb21a5", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9394.002899999994, "isoelectric_point": 9.663955879211429 }, "id": "LMJBGEANGM_326", "locus": "GCA013368015_00292" }, { "type": "cds", "sequence": "contig_1", "start": 161196, "stop": 161609, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATAGTGATTACAATTACGTTGATAAATCGCTTTGGTTAACATCTCATCCTAAAAGAACCGATGAATCCGAATTAAATTATTTTAAGTTGTTTCAGTCAGATGTAAGAGACTGGTTTGTTCCTTTTAATTTCTTAAGTCAAGGAATTCAATTGAAAAAGATTGAAAAAAATGAAAATAATATTTTTACTAAGACGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAA", "aa": "MNNSDYNYVDKSLWLTSHPKRTDESELNYFKLFQSDVRDWFVPFNFLSQGIQLKKIEKNENNIFTKTLNLEFKSTFYTIVKNKLILKNKSSYEYAIYVPFFTNKDKTIKDGKEIYQPRVFEYSCPITFVNSNYKKFK", "aa_hexdigest": "fdd2ad20be1c83f85938f8f4fa36fdff", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16541.665799999984, "isoelectric_point": 9.350252723693849 }, "id": "LMJBGEANGM_327", "locus": "GCA013368015_00293" }, { "type": "cds", "sequence": "contig_1", "start": 161670, "stop": 162197, "strand": "-", "frame": 1, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "COG:COG0242", "COG:J", "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "SO:0001217", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_D1J7P7" ], "nt": "ATGTTTAAATTTGAAGATGTTAAATTAGTTCAATTACCAGAAAAAGTATTGAGAAATAAATCAAAGAACGTTGAACTACCATTGACCGCAGAAGATGATTTATTAATACAAAAAATGATTTTTCATGTTGATGATAGTCAAACTCCTCAAACAAAATTTAGACCTGCCGTAGGAGTTGCTGCAGTTCAATATGGAATATTGAAAAATATTTTTTATGTAATGGTAAAAGACGATCAAAATAATTATATTTTTAGAGATGCTTTAATAAATCCAATAATGCTTTCACATTCAAACCATAAAATATCATTGCAAGAAGGGGAGGGATGTTTAAGTGTTAAGGAAAGTGATGCAGGTCAAGAAGGTTTTGTATATAGATATTCAAGAGTTGTAATAAAAGCATATTCGTATTTTGAAAAAAAAGAAAAAATTTATGATGTTTCCGGCTATCCGGCAATTGTATTGCAACACGAATATGACCACTTGCAAGGAAAATTATTTATAGACAGAATCAATAAAAATCAACCTTGA", "aa": "MFKFEDVKLVQLPEKVLRNKSKNVELPLTAEDDLLIQKMIFHVDDSQTPQTKFRPAVGVAAVQYGILKNIFYVMVKDDQNNYIFRDALINPIMLSHSNHKISLQEGEGCLSVKESDAGQEGFVYRYSRVVIKAYSYFEKKEKIYDVSGYPAIVLQHEYDHLQGKLFIDRINKNQP", "aa_hexdigest": "4356b018542dd2d3bbac879a184fc64a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7P7", "query_cov": 1.0, "subject_cov": 0.9459459459459459, "identity": 0.9940000000000001, "score": 346.0, "evalue": 2.35e-120, "valid": true, "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "cog_id": "COG0242", "cog_category": "J", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "LMJBGEANGM_328", "locus": "GCA013368015_00294" }, { "type": "cds", "sequence": "contig_1", "start": 162475, "stop": 162750, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTGTCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTGCCAAAGCTAATTGCAAAAGCATATCCCGAAAGAGTTATTAGATCAACGTGTTGA", "aa": "MIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTC", "aa_hexdigest": "7766a89e40dcf13447ec1cdff86791b7", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 88, "aa_cov": 0.967032967032967, "hmm_cov": 1.0, "evalue": 9.28290818378723e-20, "score": 66.57260131835938, "start": 2, "stop": 89 } ], "seq_stats": { "molecular_weight": 10111.135399999988, "isoelectric_point": 10.17938060760498 }, "id": "LMJBGEANGM_329", "locus": "GCA013368015_00295" }, { "type": "cds", "sequence": "contig_1", "start": 162844, "stop": 163386, "strand": "+", "frame": 1, "gene": "mpfA", "product": "Hemolysin C", "db_xrefs": [ "COG:COG1253", "COG:PT", "SO:0001217", "UniRef:UniRef50_A0A077L6Q7", "UniRef:UniRef90_D1J7P8" ], "nt": "ATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAACGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGCAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCTGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGGAAGGCCAGGGCACAAATGGGGTTCGTCATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATTATTGAAGAAGTTGTAGGCGAAATATACGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGA", "aa": "MKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTI", "aa_hexdigest": "692cbb311716660370230c394aa8b159", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "mpfA" ], "truncated": "both", "pseudogene": { "start": 162544, "stop": 163593, "observations": { "mutation": [ 162748, 162769, 163384, 163399, 163456, 163582 ], "stop": [ 162748, 162769, 163384, 163399, 163456, 163582 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P8", "query_cov": 1.0, "subject_cov": 0.4147465437788018, "identity": 1.0, "score": 342.0, "evalue": 3.08e-115, "gene_start": 1, "gene_end": 180, "reference_start": 186, "reference_end": 365, "reference_sequence": "MPEPLSGNYNTLNSASGASTSTGAPWQYILYSIILFLLILFSAIFSASETAYTTVSRAKIENMIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTCWFIEALYWIFFPITYPISKIGKKIYITNTEEDMKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTIWKRFEWDEILNLEAPNDEDLNLSNWLKNRVGHELHVNTKYTYEDKIDFQVISLKNKDQKFDIIDIDWSK" }, "paralog": false, "identity": 0.9828080229226361, "score": 654.0, "evalue": 1.92e-240, "description": "Internal stop codon at 162748, 162769, 163384, 163399, 163456, 163582. Nonsense mutation around 162748, 162769, 163384, 163399, 163456, 163582.", "psc": { "uniref90_id": "UniRef90_D1J7P8", "gene": "MpfA", "product": "Hemolysin C", "uniref50_id": "UniRef50_A0A077L6Q7", "cog_id": "COG1253", "cog_category": "PT", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P8", "UniRef:UniRef50_A0A077L6Q7", "COG:COG1253", "COG:PT" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6Q7" ], "product": "Hemolysin C" } }, "id": "LMJBGEANGM_330", "locus": "GCA013368015_00296" }, { "type": "cds", "sequence": "contig_1", "start": 163768, "stop": 164208, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02990", "RefSeq:WP_175936672.1", "SO:0001217", "UniParc:UPI0015894365", "UniRef:UniRef100_UPI0015894365", "UniRef:UniRef50_B3PN60", "UniRef:UniRef90_D1J7P9" ], "nt": "ATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTTCAACAAAGGCAGAACCTCATCTAATGTCTGAATTAACAAGAAAGCTTAATATTGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAG", "aa": "MSNYEIMILTNPKTTDEELQNLVFSVLDKNSSKFERLERNETAYPIKKLTHVNYFLISTKAEPHLMSELTRKLNIDKSVLRSLIINLDSEKGLKPRKVSKKFNKRIPLNRENKKPYVKNNETSKDASEEKKVARKPRTSKVLDEKK", "aa_hexdigest": "d12288d0ec03cd201983cd8d0c88def6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0015894365", "ncbi_nrp_id": "WP_175936672.1", "uniref100_id": "UniRef100_UPI0015894365", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015894365", "RefSeq:WP_175936672.1", "UniRef:UniRef100_UPI0015894365" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015894365", "uniref90_id": "UniRef90_D1J7P9" }, "psc": { "uniref90_id": "UniRef90_D1J7P9", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_B3PN60", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN60" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "LMJBGEANGM_331", "locus": "GCA013368015_00297" }, { "type": "cds", "sequence": "contig_1", "start": 164237, "stop": 164395, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00436.31" ], "nt": "ATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGA", "aa": "MNKVFLIGRLSAKPYKNVTPSNVEYSRFTMAIRREYAAPNSEPVVDFVPCVA", "aa_hexdigest": "e3643d89a9a5ba85e9b34a3c195bc11e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00436.31", "name": "SSB", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.2537681027159474e-09, "score": 32.86436462402344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5877.7719000000025, "isoelectric_point": 9.426970100402833 }, "id": "LMJBGEANGM_332", "locus": "GCA013368015_00298" }, { "type": "cds", "sequence": "contig_1", "start": 164555, "stop": 164695, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGA", "aa": "MSENRRKNNNGKEFNIATEESNTIDNNLENNHQSNESNDNPFDQLD", "aa_hexdigest": "27e71f40877bf2643a03d5e9efb89bb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.431800000002, "isoelectric_point": 4.394188499450684 }, "id": "LMJBGEANGM_333", "locus": "GCA013368015_00299" }, { "type": "cds", "sequence": "contig_1", "start": 164713, "stop": 165012, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_012855391.1", "SO:0001217", "UniParc:UPI0001BC20BE", "UniRef:UniRef100_D1J7Q1", "UniRef:UniRef50_D1J7Q1", "UniRef:UniRef90_D1J7Q1" ], "nt": "ATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGCAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCGGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAGCAGAATAA", "aa": "MARIVRKKPFIRKRPCQFCLSKEPVVYIDYKNEEVLSKLVNLQGKILSSRITGTCAKHQRAVALAIKRARFVGILPYIGLVKRESKKETVKTEKVEKAE", "aa_hexdigest": "8ab7f0f5f7af77f919b7787eca37ee67", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20BE", "ncbi_nrp_id": "WP_012855391.1", "uniref100_id": "UniRef100_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20BE", "RefSeq:WP_012855391.1", "UniRef:UniRef100_D1J7Q1" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Q1", "uniref90_id": "UniRef90_D1J7Q1" }, "psc": { "uniref90_id": "UniRef90_D1J7Q1", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_D1J7Q1", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q1" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "LMJBGEANGM_334", "locus": "GCA013368015_00300" }, { "type": "cds", "sequence": "contig_1", "start": 165848, "stop": 166576, "strand": "+", "frame": 2, "gene": "rarA", "product": "ATPase, AAA family", "db_xrefs": [ "COG:COG2256", "COG:L", "KEGG:K07478", "SO:0001217", "UniRef:UniRef50_A0A077L8I4", "UniRef:UniRef90_D1J7Q3" ], "nt": "ATGGATTCTAACTTAGCAAATTTAATAAGGCCAAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTACGGAAAGCCAGGTACTGGAAAAACAACTATTTCCTATATTCTTGCCAATGCCTTGAAAGTAAATTACGAATATTTTAATGCGGCTATTGAAAATAAAGAAGATCTTGTACATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGCTACCTTATCTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCCGCATTACGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGAGTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTAATTATTTAGCCTTGCAGGCTAATGGCGACTTTAGATCAGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCATTGGAGGCAATTAAAAAGATTATTCCCTCAATTCAATTTAGTTCAGATGAATCAGGTGATAATCATTATGATTATTTAAGTGCTTTTCATAAATCACTACGCGGTTCGGACATTGATGCAGCATTATATTGA", "aa": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQELSSCLKNACLKTFGVDLNKDIANYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALY", "aa_hexdigest": "69262ff2933040756a68c317092948f9", "start_type": "ATG", "rbs_motif": null, "genes": [ "rarA" ], "truncated": "3-prime", "pseudogene": { "start": 165848, "stop": 166879, "observations": { "mutation": [ 166574 ], "stop": [ 166574 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q3", "query_cov": 1.0, "subject_cov": 0.599009900990099, "identity": 0.992, "score": 478.0, "evalue": 3.34e-168, "gene_start": 1, "gene_end": 242, "reference_start": 1, "reference_end": 242, "reference_sequence": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALYWGFIIIKSGDFDGLFRRMLCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQYWDLIKNKGEK" }, "paralog": false, "identity": 0.9912536443148688, "score": 676.0, "evalue": 1.08e-248, "description": "Internal stop codon at 166574. Nonsense mutation around 166574.", "psc": { "uniref90_id": "UniRef90_D1J7Q3", "gene": "RarA", "product": "ATPase, AAA family", "uniref50_id": "UniRef50_A0A077L8I4", "cog_id": "COG2256", "cog_category": "L", "kegg_orthology_id": "K07478", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q3", "UniRef:UniRef50_A0A077L8I4", "COG:COG2256", "COG:L", "KEGG:K07478" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L8I4" ], "product": "ATPase" } }, "id": "LMJBGEANGM_335", "locus": "GCA013368015_00301" }, { "type": "cds", "sequence": "contig_1", "start": 166628, "stop": 167032, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12002.13" ], "nt": "TTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCAGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGCTCATCAACATTAATAAAATCTATAATTGATAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATATTGA", "aa": "MCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQY", "aa_hexdigest": "0d568cf3f41a63fd024eea087f9fb425", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12002.13", "name": "MgsA_C", "length": 132, "aa_cov": 0.9850746268656716, "hmm_cov": 1.0, "evalue": 4.6788152382900023e-42, "score": 139.26321411132812, "start": 2, "stop": 133 } ], "seq_stats": { "molecular_weight": 15219.24799999998, "isoelectric_point": 6.890670585632326 }, "id": "LMJBGEANGM_336", "locus": "GCA013368015_00302" }, { "type": "cds", "sequence": "contig_1", "start": 167064, "stop": 167381, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02912.23" ], "nt": "ATGGAATTTGATTTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTCAACAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAGAACTTGGTAAAAAAATTCAACTGTTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACTGGAGGAATTCATCCAATTAATTTGGTTGCAAATAGATTTAGAGAATGA", "aa": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFRE", "aa_hexdigest": "399641ab99a851063a0cfdba480224fb", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02912.23", "name": "Phe_tRNA-synt_N", "length": 64, "aa_cov": 0.6, "hmm_cov": 1.0, "evalue": 4.475784458903371e-08, "score": 28.557003021240234, "start": 11, "stop": 73 } ], "seq_stats": { "molecular_weight": 12088.696599999985, "isoelectric_point": 5.212385749816893 }, "id": "LMJBGEANGM_337", "locus": "GCA013368015_00303" }, { "type": "cds", "sequence": "contig_1", "start": 167385, "stop": 167822, "strand": "+", "frame": 3, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_C5J6V7", "UniRef:UniRef90_D1J7Q4" ], "nt": "TTGGCAAATGGATATTTTGAATATACAGGGCCAGAAATAGAAAATGAAGAATATAACTTTGAACGTTTGAATATTCCTCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACGGGATTTAGTGCAAGGGCATTAGAAGAACATAAAAATCAAGAGTTCTCACAATTTGCTATTGGAAAAGTTTATAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGCAACCATTCATTTGGAACATTGATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCTTATTTCCCATTTACTGAACCTAGTGTTGAGGTTGATGTTTTGTTTAATGAAAAATGA", "aa": "MANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEK", "aa_hexdigest": "440bb23b0ffb9648cd4f870c67eeb395", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 167085, "stop": 168005, "observations": { "mutation": [ 167379, 167820 ], "stop": [ 167379, 167820 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q4", "query_cov": 1.0, "subject_cov": 0.46325878594249204, "identity": 0.993, "score": 290.0, "evalue": 6.57e-97, "gene_start": 1, "gene_end": 145, "reference_start": 108, "reference_end": 252, "reference_sequence": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFREWLLANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEKWVEVLGAGMLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK" }, "paralog": false, "identity": 0.9934640522875817, "score": 604.0, "evalue": 3.44e-223, "description": "Internal stop codon at 167379, 167820. Nonsense mutation around 167379, 167820.", "psc": { "uniref90_id": "UniRef90_D1J7Q4", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_C5J6V7", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q4", "EC:6.1.1.20", "UniRef:UniRef50_C5J6V7", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_C5J6V7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6V7" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "LMJBGEANGM_338", "locus": "GCA013368015_00304" }, { "type": "cds", "sequence": "contig_1", "start": 167844, "stop": 168005, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGTTGCATGAAAATGTTTTAAAAATGGCTGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGACCAAAGATTTTTAAAACAATTTAAATAA", "aa": "MLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK", "aa_hexdigest": "247ce24247d8c02fcba2a60b9e877202", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 52, "aa_cov": 0.9811320754716981, "hmm_cov": 1.0, "evalue": 1.0234166449301633e-19, "score": 66.50789642333984, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 6107.0264, "isoelectric_point": 6.533664894104004 }, "id": "LMJBGEANGM_339", "locus": "GCA013368015_00305" }, { "type": "cds", "sequence": "contig_1", "start": 168017, "stop": 168730, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTATTTTCTTATAAAAAAATAGTTAAATTGGCAAATATAACTGATAAAAGTGTAGAAGAGGTTGTTGGAGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTTTAGATGCTGAAGGTATGAAATTCTGTCATGTATTAAAAGCATACAAAAATCCTAATTCTGATCGTTTAACGGTTTGTGAAGTTGAATTTGGCAATGGAAATCATTCAATAATTCAAACTAATGCAACAAACATGCATGAGGGCGATTATGTAATGGCTTATGTTCCTGGATCGAGATCTGGAAAAAATATATTTGCTGCCAAGGAATTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCCTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTGAATATTTTGATCTTGATGATTATTTGATTGATGTAACAATTTTATCCAATAGAGCTGATGCTTTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCTTATATTGAACCTCTTTCAAAACCAAAACCTACCTTGGAAAGCAATATTGTTGTAAGCAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCTATTACTAAACAACCTGCTATTTCTCTTCAAGATGAATTTCTTTTATGA", "aa": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLSKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLL", "aa_hexdigest": "de88bd3490aae3b5698502f05a59994e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001F47B76D", "query_cov": 1.0126582278481013, "subject_cov": 1.00418410041841, "identity": 0.504, "score": 223.0, "evalue": 7.71e-70, "valid": false, "uniref50_id": "UniRef50_A0AAV4HE63" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 82, "aa_cov": 0.3291139240506329, "hmm_cov": 0.9146341463414634, "evalue": 1.8435789663750265e-09, "score": 33.136253356933594, "start": 46, "stop": 123 } ], "seq_stats": { "molecular_weight": 26460.973900000034, "isoelectric_point": 4.757219123840332 }, "id": "LMJBGEANGM_340", "locus": "GCA013368015_00306" }, { "type": "cds", "sequence": "contig_1", "start": 168776, "stop": 170155, "strand": "+", "frame": 2, "gene": "eMAP", "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "COG:COG0073", "COG:J", "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A448ZZ89", "UniRef:UniRef90_D1J7Q5" ], "nt": "TTGGTCTTATTATACGCAGGAGTTCCTTGTCACGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGTAATGAAACATTAAATGTTTTAGGCAATAAACAGGTTACATTAAACAACAATTTGGTTGTAAAAAACGGAGATCAAACCGTTTCAATAGCGAGTGTTATAGGCTTAGAAAATTATGAATATAGACCTGATGATGGACATTGCGTCTTTGAATTAGCATCATTTTCATTAAAAGAAGTTAGAAAAAGTATAAAAACAGTCAAATTAGAAACGTTAGCATCTTTACGTGGTTCAAAAGAAATATCAAGCGGTCAAGTAGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCGCCTAAGCTAAGAAAAACAAATATATTAATTGATAGCCATTATATTACTAAATATGCAGGCTTCAATATTACAAAAACAAAAAAATATATTCAAGTTTTGAATCAATTGACTCTTTTGGGCTTTAAAATAAAGGAAGATTTATCATCAGTAATTTTTCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTGTTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAACAAAGAATTTAAGTTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTTAATCCATTTGATTTTAAAACTAATTTCAATGCAATTGCAGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTGGATACAAATATCAAACAAGGCTTGCCGGCTGCTTCTTATTTTGAAATTGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGACATTTGTTCAATAACTAATGAAACATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAACGAATTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGCGAAGTCTTTTTGAATAAAATTAATAACAACGTAATTAAATATAAAAATTACAAGCACGCACCTCTTAAATCTAGGGATGTTACTATATCCGTTTTAGATAAACAATCTATTGAAGAAACAATTAAAAAAATACAAAGTATTTTTGGCGTTTACAAAATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGA", "aa": "MVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDDGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPAASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLNKINNNVIKYKNYKHAPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILED", "aa_hexdigest": "32f05d808a47bc074160388b3be06753", "start_type": "TTG", "rbs_motif": null, "genes": [ "eMAP" ], "truncated": "both", "pseudogene": { "start": 168476, "stop": 170191, "observations": { "mutation": [ 168728, 170153 ], "stop": [ 168728, 170153 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q5", "query_cov": 1.0, "subject_cov": 0.6339779005524862, "identity": 0.985, "score": 886.0, "evalue": 0.0, "gene_start": 1, "gene_end": 459, "reference_start": 254, "reference_end": 712, "reference_sequence": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLSKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLLWKHGIKTFRNAVDITNLVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDGGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPDASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLDKMNSNVIKYKNYKHTPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILEDWATKKFDSIFNK" }, "paralog": false, "identity": 0.9859894921190894, "score": 1107.0, "evalue": 0.0, "description": "Internal stop codon at 168728, 170153. Nonsense mutation around 168728, 170153.", "psc": { "uniref90_id": "UniRef90_D1J7Q5", "gene": "EMAP", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A448ZZ89", "cog_id": "COG0073", "cog_category": "J", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q5", "EC:6.1.1.20", "UniRef:UniRef50_A0A448ZZ89", "COG:COG0073", "COG:J", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ89" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "LMJBGEANGM_341", "locus": "GCA013368015_00307" }, { "type": "cds", "sequence": "contig_1", "start": 170289, "stop": 170474, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTCGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCTGAAACTTTTAGCAGTGGTTCGTTGACTATCGATAGAGCGTTAGGAATAAATGGATGA", "aa": "MKETTNDNLQKEKSIIAETLKEIEKKFGKEAVMILGEKPSIIPETFSSGSLTIDRALGING", "aa_hexdigest": "c9f41f4d8b6240cde5449c81972c0dfd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6693.649199999999, "isoelectric_point": 5.342262840270995 }, "id": "LMJBGEANGM_342", "locus": "GCA013368015_00308" }, { "type": "cds", "sequence": "contig_1", "start": 170535, "stop": 171176, "strand": "+", "frame": 3, "gene": "recA", "product": "Protein RecA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "nt": "TTGCACGCAATTGCTGAAATACAAAAAACTGGCGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCCGGAGCAATAGGACTAATCGTTGTTGATTCGGTTGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCTTTAAGAAAAATAACTGGATCATTATCAAAAAACAAGACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAATGGAGACATCACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCATATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAAGGCTCGTGA", "aa": "MHAIAEIQKTGGIAAFIDAEHSIDPIYAKNLGVDIDNLILSQPDSGEQALEIVDTLSKSGAIGLIVVDSVAALVPEAELNGEMKDQVVGAQARLMSKALRKITGSLSKNKTTVIFINQIREKIGVIFGNPETTTGGRALKFYSSIRLEVRKIQNVTNNGDITGNQIKCKVVKNKLAAPYKTAQIDIIFSKGISKELEIINLAEELGILVKKGS", "aa_hexdigest": "b0f1378336773daef7e2d17b28aa8210", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A0D5BUH9", "query_cov": 0.9765258215962441, "subject_cov": 0.9244444444444444, "identity": 0.625, "score": 266.0, "evalue": 9.97e-88, "valid": false, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_A0A7U9RV21", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9RV21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "LMJBGEANGM_343", "locus": "GCA013368015_00309" }, { "type": "cds", "sequence": "contig_1", "start": 171342, "stop": 171566, "strand": "+", "frame": 3, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCGGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCGGGAAGAAAGGGTCTTGAGCCTAAAGATTATGAAAGATTAAAAAGAGCGGGAATAAATGCATTTACATTAGGAAATCACGTTTGA", "aa": "MKNENIKILFLGDIFGLPGIRFVEQQLPNIIKQNNIDFVIAQAENVSGRKGLEPKDYERLKRAGINAFTLGNHV", "aa_hexdigest": "e96ff80ddec4e9e058c49ad98258ca6a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9324324324324325, "subject_cov": 1.0, "identity": 0.551, "score": 91.3, "evalue": 7.99e-23, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "LMJBGEANGM_344", "locus": "GCA013368015_00310" }, { "type": "cds", "sequence": "contig_1", "start": 171927, "stop": 172148, "strand": "+", "frame": 3, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "nt": "TTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGTCCAAGAGATTGCGCTATTGGTTCTGACTTTCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAAGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACTAAAGATCCGACAGCAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAA", "aa": "MPKGTYFVTDAGMCGPRDCAIGSDFQEVYEKMRYAAFSAFKVSKNKCQLNGVIFTLTKDPTAKHIELVNIKEQ", "aa_hexdigest": "442d0c506a340999cb8e039bf86e36d6", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PKR0", "query_cov": 0.9863013698630136, "subject_cov": 0.9863013698630136, "identity": 0.667, "score": 107.0, "evalue": 2.36e-29, "valid": false, "product": "Putative metallophosphoesterase", "uniref50_id": "UniRef50_A0A3B0PKR0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PKR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "LMJBGEANGM_345", "locus": "GCA013368015_00311" }, { "type": "cds", "sequence": "contig_1", "start": 172150, "stop": 172410, "strand": "+", "frame": 1, "gene": "nusB", "product": "N utilization substance B-like protein(Protein nusB)", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J7Q8", "UniRef:UniRef90_D1J7Q8" ], "nt": "ATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGAATCTTTCAACACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCCAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATATGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGA", "aa": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKNLSTQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISED", "aa_hexdigest": "66e25f4c0388727fdb7a98a6c105d232", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nusB" ], "truncated": "3-prime", "pseudogene": { "start": 172150, "stop": 172623, "observations": { "mutation": [ 172408, 172414 ], "stop": [ 172408, 172414 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q8", "query_cov": 1.0, "subject_cov": 0.5477707006369427, "identity": 0.988, "score": 162.0, "evalue": 8.19e-50, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKNLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISEDWEWNRILPLSRAIIIYGEYELLFNDPRVVINEMVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN" }, "paralog": false, "identity": 0.9808917197452229, "score": 301.0, "evalue": 8.73e-107, "description": "Internal stop codon at 172408, 172414. Nonsense mutation around 172408, 172414.", "psc": { "uniref90_id": "UniRef90_D1J7Q8", "gene": "NusB", "product": "N utilization substance protein B homolog(Protein nusB)", "uniref50_id": "UniRef50_D1J7Q8", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q8", "UniRef:UniRef50_D1J7Q8", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q8" ], "product": "N utilization substance protein B homolog(Protein nusB)" } }, "id": "LMJBGEANGM_346", "locus": "GCA013368015_00312" }, { "type": "cds", "sequence": "contig_1", "start": 172504, "stop": 172623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAG", "aa": "MVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN", "aa_hexdigest": "348a48353fc4b62036a2aff9bc7366ba", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4585.2389, "isoelectric_point": 9.399055290222169 }, "id": "LMJBGEANGM_347", "locus": "GCA013368015_00313" }, { "type": "cds", "sequence": "contig_1", "start": 172607, "stop": 173341, "strand": "+", "frame": 2, "gene": "yqxC", "product": "Hemolysin A", "db_xrefs": [ "COG:COG1189", "COG:J", "EC:2.1.1.226", "EC:2.1.1.227", "KEGG:K06442", "RefSeq:WP_175936674.1", "SO:0001217", "UniParc:UPI001589BC35", "UniRef:UniRef100_UPI001589BC35", "UniRef:UniRef50_A0A077LAY8", "UniRef:UniRef90_D1J7Q9" ], "nt": "ATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAAATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTCGAAGAAACTGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAAACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGCGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCTTGTGATGTTTCATTTATAAGTTTGAAATATGTATTTGAGGTTTGCAATAAATTATTAAATAATAATGTAAATGTAGTTTTTTTAATTAAACCCCAATTTGAGGCTTCTAGCAAATATGTTGAACCTGGAGGGTATGTTAATCAAGAAAATCATAAATATATTACTGATAAAGTAATTTCATATGCGAAAAATAATGGCTTTGTTTTGATTAATTTAATAAAAAGTCCTATTTTAGGAGAAAAATCAAAAAACATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAA", "aa": "MKKKISEIITNIFKYDLKISEALIREGRVIVNNEKIIIPSLKFEETDVDIRFINMKLPYVSRGAYKLLEAIEKFNINLTDKICLDIGSSTGGFVDVMLRHGAKKVYAVDCGTNQLAYELRINDKVVVYEKTNLKNLKKEMFLENIEFVSCDVSFISLKYVFEVCNKLLNNNVNVVFLIKPQFEASSKYVEPGGYVNQENHKYITDKVISYAKNNGFVLINLIKSPILGEKSKNIEYLGFFRKDS", "aa_hexdigest": "8ba6b8518940b232ec3c889c8da70b3d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001589BC35", "ncbi_nrp_id": "WP_175936674.1", "uniref100_id": "UniRef100_UPI001589BC35", "db_xrefs": [ "SO:0001217", "UniParc:UPI001589BC35", "RefSeq:WP_175936674.1", "UniRef:UniRef100_UPI001589BC35" ] }, "ips": { "uniref100_id": "UniRef100_UPI001589BC35", "uniref90_id": "UniRef90_D1J7Q9" }, "psc": { "uniref90_id": "UniRef90_D1J7Q9", "gene": "YqxC", "product": "Hemolysin A", "ec_ids": [ "2.1.1.226", "2.1.1.227" ], "uniref50_id": "UniRef50_A0A077LAY8", "cog_id": "COG1189", "cog_category": "J", "kegg_orthology_id": "K06442" }, "pscc": { "uniref50_id": "UniRef50_A0A077LAY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LAY8" ], "product": "Hemolysin A" }, "genes": [ "yqxC" ], "id": "LMJBGEANGM_348", "locus": "GCA013368015_00314" }, { "type": "cds", "sequence": "contig_1", "start": 173341, "stop": 174033, "strand": "+", "frame": 1, "gene": "csdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "db_xrefs": [ "COG:COG0520", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZVE8", "UniRef:UniRef90_D1J7R0" ], "nt": "ATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGGTATTGTATATTTCGATAATTCAGCACTCACTTTTAAACCACAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGCGTTTTAGGAATACAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACCATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTTAAAAACAATAATATAACATTTAAATATGCAAAAGACAACGAGGAAGTTCTAGATTTAATAAATGAAAAAACAAGGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTAAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCTTATTTGATAAATGATGCCGCACAGGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGCAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAAAAAAAATAGTTCCTCAAAAATGA", "aa": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVMHETRKKIADLIDASADQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTRLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLKKIVPQK", "aa_hexdigest": "9c363ccaa2cbafc27b0cbd638f745025", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "csdA" ], "truncated": "3-prime", "pseudogene": { "start": 173341, "stop": 174336, "observations": { "mutation": [ 174031, 174073 ], "stop": [ 174031, 174073 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R0", "query_cov": 1.0, "subject_cov": 0.5912596401028277, "identity": 0.987, "score": 447.0, "evalue": 2.55e-156, "gene_start": 1, "gene_end": 230, "reference_start": 1, "reference_end": 230, "reference_sequence": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVIHETRKKIADLIDASANQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTRLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLEKIVPQKWGGGQVQSIGSGCEWSAKNDIKKFEPGTPNFAGIFQFNAAIDFINSLGYELINKLETAVANYCYDELKTIDGIEIDSKRGDKIILFNIKNIPSQDIASYLGHNNIYVRSGVFCAHKFHELKQYSNSYVRISISFYNDYNDVDKFIKLLKEMGANFLEFL" }, "paralog": false, "identity": 0.9848942598187311, "score": 645.0, "evalue": 8.32e-237, "description": "Internal stop codon at 174031, 174073. Nonsense mutation around 174031, 174073.", "psc": { "uniref90_id": "UniRef90_D1J7R0", "gene": "CsdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "uniref50_id": "UniRef50_A0A448ZVE8", "cog_id": "COG0520", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R0", "UniRef:UniRef50_A0A448ZVE8", "COG:COG0520", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVE8" ], "product": "Probable cysteine desulfurase" } }, "id": "LMJBGEANGM_349", "locus": "GCA013368015_00315" }, { "type": "cds", "sequence": "contig_1", "start": 174497, "stop": 174946, "strand": "+", "frame": 2, "gene": "iscU", "product": "Aminotransferase protein U-like protein (Nitrogenfixation protein NifU)", "db_xrefs": [ "COG:COG0822", "COG:O", "RefSeq:WP_044420484.1", "SO:0001217", "UniParc:UPI0005CB3A02", "UniRef:UniRef100_UPI0005CB3A02", "UniRef:UniRef50_D1J7R1", "UniRef:UniRef90_D1J7R1" ], "nt": "TTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGACGATATTAAGCTAAATTTTTTATTTGATGAAAAAAATATTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCTGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATGAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGTGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAA", "aa": "MNFYSNIEKQRIIFEAYENPKFKIKNDEDFKKTDKTLFEHSNVCVDDIKLNFLFDEKNILKKVEYCAKGCSIFLSSVELMIQQILNKNKLEINEILNEYKTMIDTSTISQRGGEILGKLVVYQNVRVHLNRLECASIIFRLIKKAVSNE", "aa_hexdigest": "0d67117469ded744b0b422062cc2c597", "start_type": "TTG", "rbs_motif": "GGxGG", "ups": { "uniparc_id": "UPI0005CB3A02", "ncbi_nrp_id": "WP_044420484.1", "uniref100_id": "UniRef100_UPI0005CB3A02", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CB3A02", "RefSeq:WP_044420484.1", "UniRef:UniRef100_UPI0005CB3A02" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CB3A02", "uniref90_id": "UniRef90_D1J7R1" }, "psc": { "uniref90_id": "UniRef90_D1J7R1", "gene": "IscU", "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)", "uniref50_id": "UniRef50_D1J7R1", "cog_id": "COG0822", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_D1J7R1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R1" ], "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)" }, "genes": [ "iscU" ], "id": "LMJBGEANGM_350", "locus": "GCA013368015_00316" }, { "type": "cds", "sequence": "contig_1", "start": 174939, "stop": 175832, "strand": "+", "frame": 3, "gene": "dinP", "product": "DNA-damage repair protein MucB", "db_xrefs": [ "COG:COG0389", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7R2", "UniRef:UniRef90_D1J7R2" ], "nt": "ATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACCTGATAGACAAGCCTGTTGTAATTGCTAAACGATACAAACGTTCAATAATTAGCGCAATGTCTTACGAGGTAAAAAACCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTGCTTATAGTAGAACCACACTATGATTTATATTCTCTAATTTCTAAAAAAATTTTTGATTATTTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTGTTAGCAATTGGGAGGAAGCTAAAAATCTTGCCCAACAAATTAAAGATGATATTTTAAAAACGTTTATGATTCCTTGTTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTTAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCACAACAATTAATAAAAAACAAAATTTCTACTTATAAAGATTTGTTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATCAATGCAACCAACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGA", "aa": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWEEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKEDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATNHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKK", "aa_hexdigest": "a4a4ea24d4e8a8b734a76fef0db233f1", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dinP", "mucB" ], "truncated": "3-prime", "pseudogene": { "start": 174939, "stop": 176135, "observations": { "mutation": [ 175830, 175917 ], "stop": [ 175830, 175917 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R2", "query_cov": 1.0, "subject_cov": 0.7105263157894737, "identity": 0.99, "score": 582.0, "evalue": 8.23e-208, "gene_start": 1, "gene_end": 297, "reference_start": 1, "reference_end": 297, "reference_sequence": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWAEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKGDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKKWMSEQKKIDFFTNDIDIIFKIALKLFLNLWNEKECLRGLGIRICDLRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK" }, "paralog": false, "identity": 0.9874371859296482, "score": 773.0, "evalue": 9.45e-286, "description": "Internal stop codon at 175830, 175917. Nonsense mutation around 175830, 175917.", "psc": { "uniref90_id": "UniRef90_D1J7R2", "gene": "DinP", "product": "DNA-damage repair protein MucB", "uniref50_id": "UniRef50_D1J7R2", "cog_id": "COG0389", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R2", "UniRef:UniRef50_D1J7R2", "COG:COG0389", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7R2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R2" ], "product": "DNA-damage repair protein MucB" } }, "id": "LMJBGEANGM_351", "locus": "GCA013368015_00317" }, { "type": "cds", "sequence": "contig_1", "start": 175965, "stop": 176195, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGTCGATATTTCAAGAAAACAAACAAGTTAATTTGTTTGAAGACAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATACAAAATTTGAGGGTAAAATTTTAATAACAGGGAAACAATATTTGTTAGAGAATTCAATCGATATTGATAATATAAAGTTTTTAAGAAAAAACAATCAACCATATGATAAAAAAATAAATTTAGAGGAGAAAAAATAA", "aa": "MRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK", "aa_hexdigest": "53be2b029bc8566ca5d7d8503c3332c4", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9030.19379999999, "isoelectric_point": 8.925663948059086 }, "id": "LMJBGEANGM_352", "locus": "GCA013368015_00318" }, { "type": "cds", "sequence": "contig_1", "start": 176195, "stop": 176536, "strand": "+", "frame": 2, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCCGTTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTGGATAAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAGGATAAGCAAGATTATGCATCTAATGAGCATAGAATAAATATGATTAATATTGTTATAAACAAGAGTAATATGGAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAACGTTGGTAGTTTAAATAAATGA", "aa": "MKIGIFGGSFDPVHKGHILIANDAIELLKLDKVIFVPANKNPFKDKQDYASNEHRINMINIVINKSNMEVSQFETKRGGTSYTIDTVKYFAQKYPNDELYFLIGSDNVGSLNK", "aa_hexdigest": "f144102cbb877a03a0a68a6aa7a7316c", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.008849557522124, "subject_cov": 1.0178571428571428, "identity": 0.588, "score": 130.0, "evalue": 2.63e-37, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "LMJBGEANGM_353", "locus": "GCA013368015_00319" }, { "type": "cds", "sequence": "contig_1", "start": 176710, "stop": 176898, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCACTCTTTAGTAATATCATGCATAAAACCAGTTCTATACGCTAATTCGACATCAAAATTATTTCTTTTAGCCAATTGTGCAGAAAATTCAGCAGTATATCTTAAATGTTTAAATCTAGGAATAGAAAGAGTATTTTTGGCAATATTTTCTAAATATAAACAATTCTTTCCTATGTATTCTTGA", "aa": "MSHSLVISCIKPVLYANSTSKLFLLANCAENSAVYLKCLNLGIERVFLAIFSKYKQFFPMYS", "aa_hexdigest": "8c6032f08446de5a4be592873d9b2f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7027.318199999999, "isoelectric_point": 9.183988761901855 }, "id": "LMJBGEANGM_354", "locus": "GCA013368015_00320" }, { "type": "cds", "sequence": "contig_1", "start": 176960, "stop": 177124, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACACCAAACTACGGCCTATTATTGGTTGAGGGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTGAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGA", "aa": "MLHQTTAYYWLRDVYKYQNDEALQAIKIHTSLDFEISLLAKILYIADKICEGRK", "aa_hexdigest": "7c978427631ae6afb59216630af6bb0c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6435.3840999999975, "isoelectric_point": 6.702192497253418 }, "id": "LMJBGEANGM_355", "locus": "GCA013368015_00321" }, { "type": "cds", "sequence": "contig_1", "start": 177343, "stop": 178032, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0006413", "RefSeq:WP_020002689.1", "SO:0001217", "UniParc:UPI0003616783", "UniRef:UniRef100_UPI0003616783", "UniRef:UniRef50_A0A448ZXX5", "UniRef:UniRef90_D1J7R4" ], "nt": "TTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATTAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTACCGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAACAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAAAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAACAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACGAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTCCGTGGTAGAGAAAGAGCAAGAATCGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAA", "aa": "MLGCYNKEFVVFGKWINTTFLFIKEGGVIINNQKIGDDLNNNQTNRKPKSEHVINNAIPYKKVFVLGPTGEKIGVLTKEEALEKASEYNMDLVLIAIENNKPIVRILDYGKFKYDKKKRQKEVKEKQAVTVNREIRLTPLIGDHDLKTKANKTREFILDGNRVKVSVKFRGRERARIELGEEILKKFFALIEDVAKISKEATLVNDRFLDMYVEKDKRKVDTLTNKKDN", "aa_hexdigest": "19f4657fcbf6cdce778424ebb9cdfd02", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003616783", "ncbi_nrp_id": "WP_020002689.1", "uniref100_id": "UniRef100_UPI0003616783", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003616783", "RefSeq:WP_020002689.1", "UniRef:UniRef100_UPI0003616783" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003616783", "uniref90_id": "UniRef90_D1J7R4" }, "psc": { "uniref90_id": "UniRef90_D1J7R4", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_A0A448ZXX5", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXX5" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "LMJBGEANGM_356", "locus": "GCA013368015_00322" }, { "type": "cds", "sequence": "contig_1", "start": 178059, "stop": 178247, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_020002688.1", "SO:0001217", "UniParc:UPI00036BF9D9", "UniRef:UniRef100_A0A2K9YT13", "UniRef:UniRef50_P75447", "UniRef:UniRef90_D1J7R5" ], "nt": "ATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAG", "aa": "MPKMKTKSGLKKRISITASGKVKRGQAFRSHLAQNKTTKQKRHARKATTLSSSDFKRYKELI", "aa_hexdigest": "12cf694fb7c510f41385e8bf4b0e3d29", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036BF9D9", "ncbi_nrp_id": "WP_020002688.1", "uniref100_id": "UniRef100_A0A2K9YT13", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036BF9D9", "RefSeq:WP_020002688.1", "UniRef:UniRef100_A0A2K9YT13" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT13", "uniref90_id": "UniRef90_D1J7R5" }, "psc": { "uniref90_id": "UniRef90_D1J7R5", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "LMJBGEANGM_357", "locus": "GCA013368015_00323" }, { "type": "cds", "sequence": "contig_1", "start": 178518, "stop": 178619, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAA", "aa": "MLVNRKMLSELAINYPTSFAKLVDQAKSALAKK", "aa_hexdigest": "30ec095d133853a43f24cd1b5e60831b", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3680.3854, "isoelectric_point": 9.997450828552246 }, "id": "LMJBGEANGM_358", "locus": "GCA013368015_00324" }, { "type": "cds", "sequence": "contig_1", "start": 178718, "stop": 178828, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGAAAAAAAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGCTACAAAAACAAGGATATGATGTTGTTGGTTTGTTCATGAGAAATTGA", "aa": "MKKRVVLGMSGGVDSSVCGYLLQKQGYDVVGLFMRN", "aa_hexdigest": "09399c4b20bbd243650b061f6885b900", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 23, "aa_cov": 0.6388888888888888, "hmm_cov": 1.0, "evalue": 1.4199931190844455e-07, "score": 26.389699935913086, "start": 3, "stop": 25 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 4.9306801996210255e-12, "score": 41.443851470947266, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3936.6466, "isoelectric_point": 9.514002418518068 }, "id": "LMJBGEANGM_359", "locus": "GCA013368015_00325" }, { "type": "cds", "sequence": "contig_1", "start": 179114, "stop": 179830, "strand": "+", "frame": 2, "gene": null, "product": "tRNA-uridine 2-sulfurtransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "nt": "ATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAATCTTACTTTTTAGCGCAATTAAGCCCTGACCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTACGAGAAATTGCAAAACAATTAAACTTGTCAACCGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACATTTCACAAAATTTTTAGAAAATTATATTCCTGCTCAACCGGGTAATATCATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCGATGTATTACACTATAGGCCAAAGAAAAGGTTTAAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGATCATATTGACTATTTAATGTCAAATAAATTAATTGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAAATTTTAGATGATAATAAAATAGAAGTTTGCTATCCAAATACCGAAGCAGTAACTCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTCGTTGGTGGAGCAATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAA", "aa": "MGHYANAIDGELFRAQDDNKDQSYFLAQLSPDQLKKVIFPLANITKPEVREIAKQLNLSTAMKKDSTGICFIGERHFTKFLENYIPAQPGNIIDIFSGKVVGKHIGAMYYTIGQRKGLNLGGYEKPYFVVGHNLSKKEIYVTNDDHIDYLMSNKLIATNFNQISKEFNFANLTAKFRYRQKDIKITLKILDDNKIEVCYPNTEAVTPGQQVVIYDGNKVVGGAIIDKIYLNNELKTFL", "aa_hexdigest": "881a1a6eed27e99860e8cd4827a0cd23", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030845807", "query_cov": 0.8235294117647058, "subject_cov": 0.98989898989899, "identity": 0.515, "score": 198.0, "evalue": 1.06e-60, "valid": false, "product": "aminomethyltransferase beta-barrel domain-containing protein", "uniref50_id": "UniRef50_A0A380E4E9" }, "pscc": { "uniref50_id": "UniRef50_A0A380E4E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "product": "tRNA-uridine 2-sulfurtransferase" }, "genes": [], "id": "LMJBGEANGM_360", "locus": "GCA013368015_00326" }, { "type": "cds", "sequence": "contig_1", "start": 180108, "stop": 180254, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTCATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFIFNVLKLNKQNIYITYYSEDKDTYNY", "aa_hexdigest": "ee4df62ca45ebba9d68af99bee4f848e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5793.464500000002, "isoelectric_point": 4.992987251281736 }, "id": "LMJBGEANGM_361", "locus": "GCA013368015_00327" }, { "type": "cds", "sequence": "contig_1", "start": 180546, "stop": 181424, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCGACAGAAAAATTAACAAACAAACGTTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTCTAATAAGAAGAGCATATAGAGCCGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATTTATGACATCGATGTTGAAGAAGTTAAAAAAGTTATTTTGGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGCGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACTATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTACAAGAAAAAAACATTGACATCGATATGAATGAATTTAACGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCCGGAATGCAAAAAGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTGGGATATGATCACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGCGAAGAAAAAATAGATAATGCGGGCGAAGATGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGAAATAATAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGA", "aa": "MQDAPTNFDTDLFLPIIHATEKLTNKRYDINNYFTKEPIQMKINTQFKIIADHIRASVNAISDGVKPSNIGRGYIIRRLIRRAYRAGISLGITQKAFLYELVQIVKDSLIYDIDVEEVKKVILDEENLFAKTINEGEKLLNQEIEKNGTIDVKIAFKMFDTYGFPIELTREILQEKNIDIDMNEFNEFLKQHQEKSRGNIEAGMQKVINSLSLITDKISEFVGYDHTKTTSKILYLLNSEEKIDNAGEDEVSYLILDKTPFYATSGGQNHDEGYILQGNNRIEVLDVFKDKY", "aa_hexdigest": "ec15ba7dd2e5c434803c7517e52d45c0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33633.83180000003, "isoelectric_point": 5.161230659484861 }, "id": "LMJBGEANGM_362", "locus": "GCA013368015_00328" }, { "type": "cds", "sequence": "contig_1", "start": 181536, "stop": 182510, "strand": "+", "frame": 3, "gene": null, "product": "Alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "nt": "ATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAGCAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCGGTCTTGATCATAGACCAACTAAAGAAGAGATAAAACTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATTAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTATAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTAACTTCATATGATTTGGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAAAAATAATCAAAAATTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAATGCGAAGAACAACTAAGAAATGATTATCGAGCATTATTAAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATTTCATTAAGAGAAGCAAATCCTGATTCAATTATTATTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGTGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAACAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAG", "aa": "MFSALRETYPNEIIKQLGSNITYEHFTFDFGLDHRPTKEEIKLIEHKMRQYIARDVKRNYIITSLKKAEEMGAYMTIDESEYHDANYIRVVEFEGITKDLCGGTHVPHSGIIEDFKIVAVDSKGTGIYRLRVVTSYDLVRKYLLSEIQEKQKILDTLIKNNQKLNEGYNFKYTKEIISNKTSDLEELLEEYDKCEEQLRNDYRALLKQKQSEDVDLSLYNYETINDKKVFLLINIEQSLIKKVSISLREANPDSIIIILNKSKQDSYYFAVASKKYDSNAIFKNLGKVLNIHGGGNAIISQGSLVSNLKQEEIIEIIKSNLCEF", "aa_hexdigest": "0e6f92bc00df13cd3e0ed06c565116e2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZR4", "query_cov": 0.9907407407407407, "subject_cov": 0.9042253521126761, "identity": 0.5579999999999999, "score": 351.0, "evalue": 2.94e-117, "valid": false, "product": "Alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0NZR4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "product": "Alanine--tRNA ligase" }, "genes": [], "id": "LMJBGEANGM_363", "locus": "GCA013368015_00329" }, { "type": "cds", "sequence": "contig_1", "start": 182498, "stop": 182845, "strand": "+", "frame": 2, "gene": null, "product": "Putative pre-16S rRNA nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "nt": "ATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAACCATTTTAACTTAGTTATTGAAAAAGTGCTACATTATTTAAATAAAAGTGAATATCAAGGTTCAATTGGCACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACGTATATGGTCGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAATTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAAAGTGCTGGATGA", "aa": "MRILALDLGTKSCGFAISDPNNIIASGLENFRFELNHFNLVIEKVLHYLNKSEYQGSIGTIVLGYPLRMDLSKSQRTYMVERFGERLKKVVDIPIVFQDERQSTIGAQEILQSAG", "aa_hexdigest": "c85741ac8049575c22fafc9172b80e18", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00112C3C79", "query_cov": 1.0, "subject_cov": 0.8098591549295775, "identity": 0.696, "score": 161.0, "evalue": 4.79e-49, "valid": false, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_B3PM70", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [], "id": "LMJBGEANGM_364", "locus": "GCA013368015_00330" }, { "type": "cds", "sequence": "contig_1", "start": 182925, "stop": 183236, "strand": "+", "frame": 3, "gene": null, "product": "DUF1292 domain-containing protein", "db_xrefs": [ "RefSeq:WP_012855410.1", "SO:0001217", "UniParc:UPI0001BC20F6", "UniRef:UniRef100_D1J7S0", "UniRef:UniRef50_D1J7S0", "UniRef:UniRef90_D1J7S0" ], "nt": "ATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACAATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGATGGAAAATCGGATTTAATGAAGAAATTAATTGGCAACGATCAAGGTGAAAATGAAGCATAA", "aa": "MNKNIINLDVVDRQLTTSDGEKLYVIFDIEENGEHYLVLTDYDAIIFAKEQDQNLIEVTDEGEIDILVDLTMEFAENNFVLDKDGKSDLMKKLIGNDQGENEA", "aa_hexdigest": "85120146fd2db4f4c4dc4a506d74e8d6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC20F6", "ncbi_nrp_id": "WP_012855410.1", "uniref100_id": "UniRef100_D1J7S0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F6", "RefSeq:WP_012855410.1", "UniRef:UniRef100_D1J7S0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S0", "uniref90_id": "UniRef90_D1J7S0" }, "psc": { "uniref90_id": "UniRef90_D1J7S0", "product": "DUF1292 domain-containing protein", "uniref50_id": "UniRef50_D1J7S0" }, "pscc": { "uniref50_id": "UniRef50_D1J7S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S0" ], "product": "DUF1292 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_365", "locus": "GCA013368015_00331" }, { "type": "cds", "sequence": "contig_1", "start": 183226, "stop": 183945, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_012855411.1", "SO:0001217", "UniParc:UPI0001BC20F7", "UniRef:UniRef100_D1J7S1", "UniRef:UniRef50_D1J7S1", "UniRef:UniRef90_D1J7S1" ], "nt": "ATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACCATTTTAGCACGTAGACATTTCTTAAAAAGTACAATAACATCAAGTGAAGAACGAGAACTACAAAATTTAAAAACAAAAAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGATAATGTTTTAGGCGACCATTTGATTGCGTTTTTAATTAATACAATATATTTGAATAATTTTTTAAACGTTAGTCTTATTGAAAAAAATGACTATGCTTCAATTTCAATAGCAGCATTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCAAGATTTAGATTTATCTAGATTAAATTATGCTGATGAAATTTATAATCCTTCAGATATGATAATATCTTTATGTGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAA", "aa": "MKHKGSIFSANTKLGLIISVVVVFILMCVILLITILARRHFLKSTITSSEERELQNLKTKNPNYGIVLSGIKKYYDNVLGDHLIAFLINTIYLNNFLNVSLIEKNDYASISIAALSMANVYSKNQYLIQKQGSNIQNENQDLDLSRLNYADEIYNPSDMIISLCDDLSIEQQINSNLPYLSDRGMLIVSFKTIKDFKNQKNVILDNNLRYETVKISNKNVILLAKNNMNSNNENSKEQQ", "aa_hexdigest": "3ab76e736897891b0c11741903f255f3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC20F7", "ncbi_nrp_id": "WP_012855411.1", "uniref100_id": "UniRef100_D1J7S1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F7", "RefSeq:WP_012855411.1", "UniRef:UniRef100_D1J7S1" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S1", "uniref90_id": "UniRef90_D1J7S1" }, "psc": { "uniref90_id": "UniRef90_D1J7S1", "uniref50_id": "UniRef50_D1J7S1" }, "pscc": { "uniref50_id": "UniRef50_D1J7S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27233.054000000026, "isoelectric_point": 8.68165111541748 }, "id": "LMJBGEANGM_366", "locus": "GCA013368015_00332" }, { "type": "cds", "sequence": "contig_1", "start": 183945, "stop": 184415, "strand": "+", "frame": 3, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_012855412.1", "SO:0001217", "UniParc:UPI0001BC20F8", "UniRef:UniRef100_D1J7S2", "UniRef:UniRef50_B3PM73", "UniRef:UniRef90_D1J7S2" ], "nt": "ATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAATGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAA", "aa": "MANNDNKIYLTEESLKQYQEKLKYLQEVARPQVIEEIKEARNQGDLSENAEYDSARDKQAAIENEILEIQHILENVEIIKSTSTHTIRIGSTVKLININTNEISEVKIVGALDADPFNNKISNTSPLAIALNGKKVGQVIEVEAPKKYKVKIESIL", "aa_hexdigest": "64b98e0388441a4943fb2bf3806ee693", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20F8", "ncbi_nrp_id": "WP_012855412.1", "uniref100_id": "UniRef100_D1J7S2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F8", "RefSeq:WP_012855412.1", "UniRef:UniRef100_D1J7S2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S2", "uniref90_id": "UniRef90_D1J7S2" }, "psc": { "uniref90_id": "UniRef90_D1J7S2", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_B3PM73", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM73" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "LMJBGEANGM_367", "locus": "GCA013368015_00333" }, { "type": "cds", "sequence": "contig_1", "start": 184520, "stop": 185194, "strand": "+", "frame": 2, "gene": "trmN6", "product": "DNA methylase", "db_xrefs": [ "COG:COG4123", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_D1J7S3" ], "nt": "ATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTATTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAATAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGCGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAGCTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGCAATAAATTAGCCAAAAAATACGATTTAATTGTTGCTAATCCGCCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACACATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGTTAAGAAAATACAATTTTGAACCAAAAAGAATTCAATTAGTTTATCCCAGAATCACTGATTTACCAAAATTCTGTTTAGTTGAAGCTAGGTTTAATTCAGGTTGA", "aa": "MKNEVVKNNLGYSGDLFVYQDKSMFNYSVDTILLGNFVSINRKVRNILEVGTNNGALSIFIAARDKRLNIDAIEIQSKAVELARKNIILNKLENQINLIEGDFRMWANQKAYDCGNKLAKKYDLIVANPPYYNEDYNQQRKNSTLEQKLATHEINLNLEQLVANSARIIEQKGYLAIVLPMARYIDLICLLRKYNFEPKRIQLVYPRITDLPKFCLVEARFNSG", "aa_hexdigest": "1bdc4b0dbaa34b36576f4f70f66f78e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S3", "query_cov": 1.0, "subject_cov": 0.8615384615384616, "identity": 1.0, "score": 442.0, "evalue": 1.38e-156, "valid": true, "gene": "TrmN6", "product": "DNA methylase", "uniref50_id": "UniRef50_A0A448ZXG4", "cog_id": "COG4123", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [ "trmN6" ], "id": "LMJBGEANGM_368", "locus": "GCA013368015_00334" }, { "type": "cds", "sequence": "contig_1", "start": 185325, "stop": 185579, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "nt": "ATGAATAATAATAAAAAGACATTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTGACTACTACATTAGCTTCAGTATATAGAAATTACAAAAATCAACAAGGATATGAAACCTTGTTTATTACTGGAATTGATGAACATGGTCAAAAGATTCAAAAAAAAGCTCTTGAACAAAATATTACTCCAAAAGAATACGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGA", "aa": "MNNNKKTFYITTPIYYPSGNLHIGHLLTTTLASVYRNYKNQQGYETLFITGIDEHGQKIQKKALEQNITPKEYVDIQSQKFVDL", "aa_hexdigest": "a8533ae3282b66b6df0e210df00a162b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00283F72E3", "query_cov": 0.8809523809523809, "subject_cov": 0.8705882352941177, "identity": 0.608, "score": 94.4, "evalue": 1.11e-23, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A7X9AHZ7" }, "pscc": { "uniref50_id": "UniRef50_A0A7X9AHZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "LMJBGEANGM_369", "locus": "GCA013368015_00335" }, { "type": "cds", "sequence": "contig_1", "start": 185664, "stop": 185852, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAAAGGCTATATTTATAAATCTAAATATGAAGGTTTATATTCAATTAGCGACGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGATGGAAAATTTTACCACCCAATTTCAGGACATGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACTCCTTGA", "aa": "MEQKGYIYKSKYEGLYSISDEEFLTKTQAISKDGKFYHPISGHELQEVKEESYFFNMSKFTP", "aa_hexdigest": "44f2d0256d7935fe5526ee4f0eb1c69b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7354.149999999999, "isoelectric_point": 5.585135841369628 }, "id": "LMJBGEANGM_370", "locus": "GCA013368015_00336" }, { "type": "cds", "sequence": "contig_1", "start": 185819, "stop": 186019, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTGTTGCTTTTCAGCATCTTTAGTTTCTATTTTAATTCCCCAATCAAAAGAAACCCTTGTTATTGAAAGATCTTCTAAACCTTTATTAATAAAATTATTCATTAATTCTTTATATGTTGCTTTATTGGTAATGAAATCAGGATTTTGTTCGAAATATGACTTTATTCAAGGAGTAAATTTTGACATATTAAAGAAATAA", "aa": "MCCFSASLVSILIPQSKETLVIERSSKPLLIKLFINSLYVALLVMKSGFCSKYDFIQGVNFDILKK", "aa_hexdigest": "160a7eea1c9f619d9229f69978a60a0e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7432.932199999998, "isoelectric_point": 9.183859825134277 }, "id": "LMJBGEANGM_371", "locus": "GCA013368015_00337" }, { "type": "cds", "sequence": "contig_1", "start": 186258, "stop": 186656, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCAAAAGGCAATGTTATTGAGCCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGATAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTAGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTCCAAACCAATTGAATTCATTAGATAAAAATGTTTATTTAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAAGCTTTAAAAAGAGCAATTGAATTAAGTAAGTTTTTAAATGAATATATCGATAAAAACGAACCTTGA", "aa": "MSKSKGNVIEPIPLIEKYGAEPVKYFFASQINIDNDFSFSEELLINVLNADLANNFGNLANRTFKMVMLNFENGTTYCPNQLNSLDKNVYLEIENAYNDYKKYFDNFNADKALKRAIELSKFLNEYIDKNEP", "aa_hexdigest": "9cb6cf39961f02726da1d0363b7c0f51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15273.029699999985, "isoelectric_point": 4.756821250915528 }, "id": "LMJBGEANGM_372", "locus": "GCA013368015_00338" }, { "type": "cds", "sequence": "contig_1", "start": 186672, "stop": 186890, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "nt": "TTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAACATTTTATTAAATGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTTTAAATTTGATAACAAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAA", "aa": "MPRLNVVLNTLLNGIYAVNMMLSVVLKEKCNILLNDLQLSEFSEKMLFNYFKFDNKKFSKPNILFERIKPNN", "aa_hexdigest": "256eb6a886baffdfe25afc3f4a5ced2c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PIV2", "query_cov": 0.9444444444444444, "subject_cov": 0.9066666666666666, "identity": 0.5, "score": 62.8, "evalue": 1.61e-11, "valid": false, "product": "Methionyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A3B0PIV2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PIV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "LMJBGEANGM_373", "locus": "GCA013368015_00339" }, { "type": "cds", "sequence": "contig_1", "start": 186911, "stop": 188062, "strand": "+", "frame": 2, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "GO:0003924", "SO:0001217", "UniRef:UniRef50_Q4A6N4", "UniRef:UniRef90_D1J7S5" ], "nt": "ATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGACGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGTGGTCCTGACGGTGGCGACGGTGGCGACGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTTGCTGAATCAGGAGAAAATGGAAGGCCAAAAAATCAATATGGGGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAGGTTTTAGCAGATGTTGGCTTTGTTGGAAAACCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCCGATTTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGCCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGCGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAATAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAATGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGATGCTTGTTTGAAACATTGCAATTTGCTAAAGATTCAATCGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAACCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGCGATGATATAGATAATATATATAATAAGATACCAGTGATTTCATTAGATAATTTATGA", "aa": "MKFIDETNVIVIAGKGGDGIISFRREANVDKGGPDGGDGGDGGDVYFVGDPGQNTLLPFYYQNKLVAESGENGRPKNQYGADGQDLIVKVPLGTMVYNGDDLIADVVSQEKYLIAKGGHGGKGNPRFKSSTNTAPRICENGTPGQKFELHLVLKVLADVGFVGKPSAGKSTILGEISNAKPKIADYDFTTLVPQLGLVKYFDNSYVVADLPGLIENAHLGKGLGIQFLKHIERCKIIAHVIDFGSEEKNPIDDYEIINNELKSYNLLLEKLPQVVIANKKDLPAFSLNLSKFKEKYPNINVFEYSALMAENLDELKGCLFETLQFAKDSIAVQQIHESQNIVKITLNKKPIQIKKIDNETYELFGDDIDNIYNKIPVISLDNL", "aa_hexdigest": "1c5f1961f976a70af26923d8c945d0ee", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S5", "query_cov": 1.0, "subject_cov": 0.9033018867924528, "identity": 0.995, "score": 741.0, "evalue": 4.06e-269, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q4A6N4", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N4" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "LMJBGEANGM_374", "locus": "GCA013368015_00340" }, { "type": "tRNA", "sequence": "contig_1", "start": 188332, "stop": 188408, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 86.0, "nt": "GGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 188349, 188351 ], "id": "LMJBGEANGM_7", "locus": "GCA013368015_00341" }, { "type": "tRNA", "sequence": "contig_1", "start": 188414, "stop": 188490, "strand": "+", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 78.5, "nt": "GGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 188443, 188445 ], "id": "LMJBGEANGM_8", "locus": "GCA013368015_00342" }, { "type": "tRNA", "sequence": "contig_1", "start": 188495, "stop": 188571, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 77.9, "nt": "GGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 188529, 188531 ], "id": "LMJBGEANGM_9", "locus": "GCA013368015_00343" }, { "type": "tRNA", "sequence": "contig_1", "start": 188596, "stop": 188671, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 86.1, "nt": "GGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "LMJBGEANGM_10", "locus": "GCA013368015_00344" }, { "type": "tRNA", "sequence": "contig_1", "start": 188674, "stop": 188764, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 74.7, "nt": "GGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 188693, 188695 ], "id": "LMJBGEANGM_11", "locus": "GCA013368015_00345" }, { "type": "tRNA", "sequence": "contig_1", "start": 188771, "stop": 188846, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 69.0, "nt": "AGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "LMJBGEANGM_12", "locus": "GCA013368015_00346" }, { "type": "tRNA", "sequence": "contig_1", "start": 188848, "stop": 188924, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 74.0, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 188849, 188851 ], "id": "LMJBGEANGM_13", "locus": "GCA013368015_00347" }, { "type": "tRNA", "sequence": "contig_1", "start": 188926, "stop": 189001, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 76.9, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 188959, 188961 ], "id": "LMJBGEANGM_14", "locus": "GCA013368015_00348" }, { "type": "cds", "sequence": "contig_1", "start": 189089, "stop": 189229, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGA", "aa": "MNINSIKIAIQLFGIFGSIVTICLGIPQLIRLKKTKNTGKVSFAAF", "aa_hexdigest": "fa5f71c74dafc2ab4e51ccf2c18d046b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 35, "aa_cov": 0.7608695652173914, "hmm_cov": 1.0, "evalue": 3.468452724384329e-07, "score": 25.503488540649414, "start": 10, "stop": 44 } ], "seq_stats": { "molecular_weight": 5009.071300000001, "isoelectric_point": 10.595137214660646 }, "id": "LMJBGEANGM_375", "locus": "GCA013368015_00349" }, { "type": "cds", "sequence": "contig_1", "start": 189386, "stop": 189640, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAGGGCTATCGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGTCTATTTTTTGTTACGGTATATCAAAAGTTAAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCCGTTCTTCCAGGTAATGCACAACTTGCATTTGGACTAATATGTCCCGCCTTTACAAGTGCAGCATTTTTCCCTGGATTAATTGTTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGA", "aa": "MLIRAIVGISINQVISLIFLCLFFVTVYQKLKFGKFYPDNSIIPVLPGNAQLAFGLICPAFTSAAFFPGLIVSLIKKEEISLSP", "aa_hexdigest": "60c807b458bcf65549d7b3b4131e8543", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9207.040499999996, "isoelectric_point": 9.138925361633301 }, "id": "LMJBGEANGM_376", "locus": "GCA013368015_00350" }, { "type": "cds", "sequence": "contig_1", "start": 189906, "stop": 190262, "strand": "-", "frame": 1, "gene": "yIH1", "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1739", "COG:R", "RefSeq:WP_230588788.1", "SO:0001217", "UniParc:UPI000B272C52", "UniRef:UniRef100_A0A454C9C9", "UniRef:UniRef50_D1J7S7", "UniRef:UniRef90_D1J7S7" ], "nt": "ATGGCAATTTTAGAAATTAAAAAGTCTAAATTTATTTCTTATAAATTTAATATTAAAAGTAAAGATGATGTGAAACTTTTAAAAGAGCAATTAAAAAATCAACATCAAAAAGCAAGACATATAGTTTATGCTTATTTAGTTATTGAAAATGGCGTTCAATGTGGTGGAATGAGTGATGATGGTGAACCATCAGGAACAGCAGGCAAACCTTTATATTTTTTACTAGAAAGAAATAAATTAAATAATTGTGTGTTGTTTGTAGTTAGATATTTTGGTGGCATAAAATTAGGCGCAAGTAAATTAATTAGAGCATATTCTAAGGTTGCAAATGATGAAATTAAAAGTAGCAATCAGTAA", "aa": "MAILEIKKSKFISYKFNIKSKDDVKLLKEQLKNQHQKARHIVYAYLVIENGVQCGGMSDDGEPSGTAGKPLYFLLERNKLNNCVLFVVRYFGGIKLGASKLIRAYSKVANDEIKSSNQ", "aa_hexdigest": "f4342fd5f2bce06067aee48b618c5dc8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000B272C52", "ncbi_nrp_id": "WP_230588788.1", "uniref100_id": "UniRef100_A0A454C9C9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000B272C52", "RefSeq:WP_230588788.1", "UniRef:UniRef100_A0A454C9C9" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9C9", "uniref90_id": "UniRef90_D1J7S7" }, "psc": { "uniref90_id": "UniRef90_D1J7S7", "gene": "YIH1", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S7", "cog_id": "COG1739", "cog_category": "R" }, "pscc": { "uniref50_id": "UniRef50_D1J7S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S7" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [ "yIH1" ], "id": "LMJBGEANGM_377", "locus": "GCA013368015_00351" }, { "type": "cds", "sequence": "contig_1", "start": 190303, "stop": 191196, "strand": "+", "frame": 1, "gene": "rluA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_012855418.1", "SO:0001217", "UniParc:UPI0001BC20FE", "UniRef:UniRef100_D1J7S8", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_D1J7S8" ], "nt": "ATGTTAAAAATTATTTCAAATTATTCAGACAGAATAGATAAATATGTTGCTAATAATAGTGAGATTTCAAGAAACGATATTAAGGAATTGATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAATAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAGGTTGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGCTTAGTTGTTCACCCAGCTCCTGGGCATCATGACAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGTATGGGGGTTGTTCACAGAATCGATAAAGATACTAGCGGCTTATTATTAATTGCTAAAAACAATAAAGCTCATAACTACTTTGCTTCTCTTTTAAAAACACATGAAATTAAAAGGAGCTATTATGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCCGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCGTTAGTTAAATGTAATTTAAAAACTGGTCGTACTCACCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGAGATCCAATTTACGGTCACAAAATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAA", "aa": "MLKIISNYSDRIDKYVANNSEISRNDIKELIENGAVFVNSIKINKPKFVVKEGDEILIERLLDKQIKVEPQNIHLDIVYECDDYLIINKPSGLVVHPAPGHHDNTLVNALMYHFKNNLSDVNGLLRMGVVHRIDKDTSGLLLIAKNNKAHNYFASLLKTHEIKRSYYAIVEGKIANEELHIELPIGRDTKNRQKYAVSETNSKPAYTIVNVVKYFKMNNKDFSLVKCNLKTGRTHQIRVHLAYIKHPVYGDPIYGHKIDDFNQRLHAFALDFVDLNGNNKHFEAEMPEIMKKEITGE", "aa_hexdigest": "c66bfbcb51e14849c3255465f1b3c238", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC20FE", "ncbi_nrp_id": "WP_012855418.1", "uniref100_id": "UniRef100_D1J7S8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20FE", "RefSeq:WP_012855418.1", "UniRef:UniRef100_D1J7S8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S8", "uniref90_id": "UniRef90_D1J7S8" }, "psc": { "uniref90_id": "UniRef90_D1J7S8", "gene": "RluA", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [ "rluA" ], "id": "LMJBGEANGM_378", "locus": "GCA013368015_00352" }, { "type": "cds", "sequence": "contig_1", "start": 191420, "stop": 191569, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAAATATGCTGAACTTTTTGGCCAAATCCAAAAATTAAATCCAAATTTTCAAGGAGATGTATTCGCACTAGCCAATAATGAATATGTCAAATCTTTGGCAATGTCAGGCGTTTTACTTGTAATTTTAATTGCGGTTTATGTTTGA", "aa": "MNKYAELFGQIQKLNPNFQGDVFALANNEYVKSLAMSGVLLVILIAVYV", "aa_hexdigest": "448b2300fae38c03cd81a8f528029e4b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5448.3564, "isoelectric_point": 5.936628150939941 }, "id": "LMJBGEANGM_379", "locus": "GCA013368015_00353" }, { "type": "cds", "sequence": "contig_1", "start": 191741, "stop": 192220, "strand": "+", "frame": 2, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9", "UniRef:UniRef90_D1J7S9" ], "nt": "GTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTATTTTTAAAATGCTCTTATGTCGCAAAACTATTTTTAATTTTAAAGAGAAATTTAGCATTTAAGGCATTAAACAACTCAGGCTCAATGGATGATATTTTAAGATCATATTTCTATGGTCAAGAATCTCCTGAACCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGATGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAAATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCTAAAAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAAATCAACAATCAAAACTAGATAATTCCGATGAAGACAACAACGATGGAAGTCAAACCAGCAATTAA", "aa": "MATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPEPTLNTNNTFDSNQKTDENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKLDNSDEDNNDGSQTSN", "aa_hexdigest": "3723a6e92619cdf57031d550936ae56c", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 191441, "stop": 192220, "observations": { "mutation": [ 191567, 191714 ], "stop": [ 191567, 191714 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7S9", "query_cov": 0.9937106918238994, "subject_cov": 0.49221183800623053, "identity": 0.981, "score": 237.0, "evalue": 8.99e-76, "gene_start": 2, "gene_end": 159, "reference_start": 164, "reference_end": 321, "reference_sequence": "MKSININEYNSTNELYTKNKKVFWAWFIAWSVLFATILIFVIASFIQFAVEKQNYLNKYAELFGQIQKLNPNFQGDVFALANNEYVKSLAMSGVLLVILIAVYVWHIVSIALVAKNKDFSAYSRILTTLYLFVIIYEIITLIFSGFQSFSLAIWQVNKILNLVATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPESTLNTNNTFDSNQKTEENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKPDNSDEDNNDGSQTSN" }, "paralog": false, "identity": 0.9806949806949807, "score": 440.0, "evalue": 3.96e-158, "description": "Internal stop codon at 191567, 191714. Nonsense mutation around 191567, 191714.", "psc": { "uniref90_id": "UniRef90_D1J7S9", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7S9", "UniRef:UniRef50_D1J7S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "LMJBGEANGM_380", "locus": "GCA013368015_00354" }, { "type": "cds", "sequence": "contig_1", "start": 192263, "stop": 192358, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATCCTCCTGCTAGTTCAATAAAAAATGGTGATCAAAAATTATGA", "aa": "MNNQTSLKQHIFTFFSQNPPASSIKNGDQKL", "aa_hexdigest": "1850d34680cd35cdae2d863bb2a7a0fd", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3521.910500000001, "isoelectric_point": 9.700380516052245 }, "id": "LMJBGEANGM_381", "locus": "GCA013368015_00355" }, { "type": "cds", "sequence": "contig_1", "start": 192647, "stop": 193102, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCGCTTTCAATTGTTTTAATAATATCAATTAATTTATCACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATCCAAAGAATTAAATGATTTAAATGCCCCTGAAAATATTAAACTGGATATCAAAGCAATGGAATCACACAACCCATTAGCCCCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAGTATTGAATTATTTAAACGTTATAAAAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCTCTTGAAGTAATTGAAGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATCTAGACAACATAAATAATAAAGATCCTGAATCACAAAAAAATTCTCTAATTTTAAAAGAAATTGCTTGA", "aa": "MIALSIVLIISINLSLLAIYFVSFKEAKFVNKKNLEDELISLKYKKSKELNDLNAPENIKLDIKAMESHNPLAPRVLAKFIDFYNKMSSIELFKRYKNYLNQTFKIRYFNESLEVIEGKNEMEDNLEKYLDNINNKDPESQKNSLILKEIA", "aa_hexdigest": "f17dfa072a591aad412b37b5ea230221", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17699.3643, "isoelectric_point": 7.786249732971191 }, "id": "LMJBGEANGM_382", "locus": "GCA013368015_00356" }, { "type": "cds", "sequence": "contig_1", "start": 193112, "stop": 193255, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAAAAAGGTTAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATATGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGA", "aa": "MDKKVKIIREADKNITMSKKEFQEKYDEYVYLQRSQDPAYQQTQKNT", "aa_hexdigest": "cc941d0e9ecb8fb550071971155c5a9e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5759.439400000004, "isoelectric_point": 8.96292667388916 }, "id": "LMJBGEANGM_383", "locus": "GCA013368015_00357" }, { "type": "cds", "sequence": "contig_1", "start": 193448, "stop": 194047, "strand": "+", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_049342325.1", "SO:0001217", "UniParc:UPI00066D7B96", "UniRef:UniRef100_UPI00066D7B96", "UniRef:UniRef50_D1J7T1", "UniRef:UniRef90_D1J7T1" ], "nt": "ATGACTAAGAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCCGTAGCCCTTGTGCCAGCAACTATGGTTTCATGTTCAAAAAAAGATATTGAAGAAATAAAATTGGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAGAAATTTAGAAGTAGAATTTAATAAAATTATAATTTCATTAAAAAAAATGGATATAACACCTGATTTTAATATGGATCAATTTATTCAAGAAATGACTAAAAAATTTAGCGCTGTAGCAAATAATTTTTTGAATATAAAATACAATAAGGCCAAATTAACAAGTGAATATTATATTTCAAAAGTAAAAGCTCAGCTAATTTTGGAGCAATTTAAAAATTCTGGTATTCTTCCAGAAAAAAATGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCGACTATTAAAACCTCTGAAGAAGAGTTAAAAAATTCAATCAGATTTTTAGATGATTTAAACAAAAAATATAATGAAATTTTAAAAAAGCAAGTAATGATTTCAAAATAA", "aa": "MTKKHKRILFLSTLALPVALVPATMVSCSKKDIEEIKLDMITIAKNQMLNYLQKFESFFKNDDDPDVRNLEVEFNKIIISLKKMDITPDFNMDQFIQEMTKKFSAVANNFLNIKYNKAKLTSEYYISKVKAQLILEQFKNSGILPEKNEFAKILEQELSKVSDPTIKTSEEELKNSIRFLDDLNKKYNEILKKQVMISK", "aa_hexdigest": "f3e4c61a9eb19d49fa7634dc6e76087b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00066D7B96", "ncbi_nrp_id": "WP_049342325.1", "uniref100_id": "UniRef100_UPI00066D7B96", "db_xrefs": [ "SO:0001217", "UniParc:UPI00066D7B96", "RefSeq:WP_049342325.1", "UniRef:UniRef100_UPI00066D7B96" ] }, "ips": { "uniref100_id": "UniRef100_UPI00066D7B96", "uniref90_id": "UniRef90_D1J7T1" }, "psc": { "uniref90_id": "UniRef90_D1J7T1", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7T1" }, "pscc": { "uniref50_id": "UniRef50_D1J7T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T1" ], "product": "Lipoprotein" }, "genes": [], "id": "LMJBGEANGM_384", "locus": "GCA013368015_00358" }, { "type": "cds", "sequence": "contig_1", "start": 194209, "stop": 194625, "strand": "+", "frame": 1, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02950", "RefSeq:WP_012855422.1", "SO:0001217", "UniParc:UPI0001BC2102", "UniRef:UniRef100_D1J7T2", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A0A858U5X2" ], "nt": "ATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCTGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTGCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATTGTTCGTGGAACACAAGATGCTGCCGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAA", "aa": "MPTVAQLVKQGRKDKTTKSKAPALGKMYNSLQKKETAMPAPFKRGVCTRVATMTPKKPNSAIRKYARVRLSNGQEVTAYIPGEGHNLQEHSVVLIRGGKVKDLPGVRYTIVRGTQDAAGVNNRKQARSIYGTKKPKSN", "aa_hexdigest": "f80b852c405717dd77647c74b99dac37", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2102", "ncbi_nrp_id": "WP_012855422.1", "uniref100_id": "UniRef100_D1J7T2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2102", "RefSeq:WP_012855422.1", "UniRef:UniRef100_D1J7T2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7T2", "uniref90_id": "UniRef90_A0A858U5X2" }, "psc": { "uniref90_id": "UniRef90_A0A858U5X2", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsL" ], "id": "LMJBGEANGM_385", "locus": "GCA013368015_00359" }, { "type": "cds", "sequence": "contig_1", "start": 194656, "stop": 194964, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "nt": "ATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAACGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGA", "aa": "MSRKHKAPVRDVLADPVFNSKIITKLVNTIMLDGKKSIAENILYSAFDIIKTKTGKEPVEVFNLALENVSPQLEVRSRRVGGSNYQVPCEVSAKRKQTLALR", "aa_hexdigest": "a846a895e05ab362d597ef3c44d7d680", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A381KMK1", "query_cov": 1.0, "subject_cov": 0.8429752066115702, "identity": 0.5589999999999999, "score": 124.0, "evalue": 1.23e-34, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A381KMK1", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A381KMK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "LMJBGEANGM_386", "locus": "GCA013368015_00360" }, { "type": "cds", "sequence": "contig_1", "start": 195001, "stop": 195123, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948V4F4", "UniRef:UniRef90_J9ZBC4" ], "nt": "ATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGA", "aa": "MEEKLANELIDASNKVGGAIKKREDTHKMAESNKAFAHFR", "aa_hexdigest": "c45ebf744fe7d637b0f9acfff3932c27", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.775, "score": 69.7, "evalue": 4.19e-15, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 194950, "stop": 195123, "observations": { "mutation": [ 194962 ], "stop": [ 194962 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_J9ZBC4", "query_cov": 1.0, "subject_cov": 0.6896551724137931, "identity": 0.8, "score": 69.3, "evalue": 7.18e-15, "gene_start": 1, "gene_end": 40, "reference_start": 18, "reference_end": 57, "reference_sequence": "MALRWIVDSAYKRAGHSMEEKLAGELLDASNNTGGAVKKREDTHRMAEANKAFAHYRW" }, "paralog": false, "identity": 0.6666666666666666, "score": 79.0, "evalue": 1.2e-21, "description": "Internal stop codon at 194962. Nonsense mutation around 194962.", "psc": { "uniref90_id": "UniRef90_J9ZBC4", "product": "Ribosomal protein S7", "uniref50_id": "UniRef50_A0A948V4F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_J9ZBC4", "UniRef:UniRef50_A0A948V4F4" ] } }, "id": "LMJBGEANGM_387", "locus": "GCA013368015_00361" }, { "type": "cds", "sequence": "contig_1", "start": 195139, "stop": 195294, "strand": "+", "frame": 1, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATCATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGA", "aa": "MSREYKLEDYRNIGIMAHIDAGKTTTTERVLYHTGKIHKIGETHEGASQMD", "aa_hexdigest": "819aa66155ed42a00a7c7201fbcb4d6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.725, "score": 84.7, "evalue": 8.03e-21, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 195139, "stop": 195351, "observations": { "mutation": [ 195292 ], "stop": [ 195292 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 0.882, "score": 100.0, "evalue": 5.36e-27, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.8571428571428571, "score": 126.0, "evalue": 8.15e-40, "description": "Internal stop codon at 195292. Nonsense mutation around 195292.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "LMJBGEANGM_388", "locus": "GCA013368015_00362" }, { "type": "cds", "sequence": "contig_1", "start": 195556, "stop": 196662, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "nt": "ATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGCGCTAATGCTCACGCTATTCAATTAAATATCGGTGAAGAAAACCATTTTGTTGGAATCATTGATTTAGTTGAAATGAAAGCATATTCATATGATGGTGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGAGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGACATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAGGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGATGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGAAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTGAAAGTAACAACCACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCTGCTGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGCGCACCACAAGTTTCATATCGTGAAACTATTACAAAGACTGCCGATGTTGAAGGTATGCATAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGA", "aa": "MGANFKASVESLHKLLGANAHAIQLNIGEENHFVGIIDLVEMKAYSYDGEVDENAKEIAIPEHLKDEAHIMRAELAESLVDYDEELMTLLLEEKEVPVDILKRAIRTATISSEYFPVVCGTSFKNKGVKLMLDAIVDYLPSPLDIPPMKAYKGESEIHIPASDDEFFSALAFKVMNDPFVGNLTYFRVYSGIVSKGSYVFNSTKGEKERLSRILLMHANSRTDIDEVRTGDIAAAVGLKVTTTGDTLIDEKQKDVVLENMNFPEPVISQAIEPKTKDASEKLSLALQRLAAEDPTFKYYTDEETGQTIIAGMGELHLDIIVDRLRREFKVEVTVGAPQVSYRETITKTADVEGMHKKQSGGKGQYGHV", "aa_hexdigest": "673df4d7d54be95568c1227b72ebd123", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00255A9B97", "query_cov": 1.0, "subject_cov": 1.005464480874317, "identity": 0.503, "score": 335.0, "evalue": 3.36e-110, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A379SQ57" }, "pscc": { "uniref50_id": "UniRef50_A0A379SQ57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A379SQ57" ], "product": "Elongation factor G" }, "genes": [], "id": "LMJBGEANGM_389", "locus": "GCA013368015_00363" }, { "type": "cds", "sequence": "contig_1", "start": 196774, "stop": 197145, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "nt": "ATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGACAAGTTAGAGATAACGAAACAAGAAACGATGGAGCCCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGA", "aa": "MAIGILAGYPMIDIKATLFDGSYHEVDSSELAYKIAASKSLTKGREALGTVLLEPIMDVAVVVPEDFFGDVMGDISRRRGQVRDNETRNDGAHVIKAYIPLSEMFGYATQLRSMTTGRGTYQM", "aa_hexdigest": "96f465eb36f63e8aba1a1d4f44085104", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449AGV8", "query_cov": 1.0, "subject_cov": 0.9318181818181818, "identity": 0.772, "score": 194.0, "evalue": 3.83e-62, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A449AGV8" }, "pscc": { "uniref50_id": "UniRef50_A0A449AGV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "LMJBGEANGM_390", "locus": "GCA013368015_00364" }, { "type": "cds", "sequence": "contig_1", "start": 197491, "stop": 197706, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCAAGAAACTCGAGATAAATTAATCTTAGCCAACGCCTTGCCTTCTGTTTCGGGTGCAATGTTACAAGTTAGATTTACATTTACCTATATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTATTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTGGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGA", "aa": "MSQETRDKLILANALPSVSGAMLQVRFTFTYISNILIGFALIFYGLFPNYLWTKRGFFLATVYINITFFTF", "aa_hexdigest": "ac61b9139cf3c997c64fd839ef48418c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8214.596099999992, "isoelectric_point": 9.694836235046388 }, "id": "LMJBGEANGM_391", "locus": "GCA013368015_00365" }, { "type": "cds", "sequence": "contig_1", "start": 197932, "stop": 198255, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGACTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGCTATCATGGCAATAATTTGTTTTATAAAATTCTATTAAATTTATTTATCCCAATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCATTTTTAAGAGAAAATAAACTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAA", "aa": "MQVTNLFLIEQFNKKTTGLDIFTKTKVAIYPFLNLKYPLGYHGNNLFYKILLNLFIPIFGIIVCISVAYFLKFICKIQTYSKKDFENPAFLRENKLLIKSIKSQIFK", "aa_hexdigest": "4d283377d9f4a9083156c97c15d29ca5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.982599999981, "isoelectric_point": 9.758853340148924 }, "id": "LMJBGEANGM_392", "locus": "GCA013368015_00366" }, { "type": "cds", "sequence": "contig_1", "start": 198267, "stop": 198896, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "ATGACGGCTTTTAATTTAAAAAAATATTTTGAGTTACAAACAATTGAAATTAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGGGGCAAAATTTTTGACGATTTACATGCCTCAAGAGTATTACCCGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTAAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCAAATGACATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGATGATGTATTAAGATTAATTGACAATTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAATCAACCTTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGCTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAACAATATAGATTCTGGTTATGCAAAATTTGAAACATTTCCTGTTTGA", "aa": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIDDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPV", "aa_hexdigest": "3c65e9ca178b41be8dbaeebc1de2b15b", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0025EA5487", "query_cov": 0.9904306220095693, "subject_cov": 0.8922413793103449, "identity": 0.57, "score": 244.0, "evalue": 4.93e-79, "valid": false, "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_A0A348P2W6" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 198267, "stop": 199199, "observations": { "mutation": [ 198894 ], "stop": [ 198894 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.4247967479674797, "identity": 0.995, "score": 410.0, "evalue": 1.89e-140, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.9903225806451613, "score": 610.0, "evalue": 7.7e-222, "description": "Internal stop codon at 198894. Nonsense mutation around 198894.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "LMJBGEANGM_393", "locus": "GCA013368015_00367" }, { "type": "cds", "sequence": "contig_1", "start": 198918, "stop": 199745, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "TTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATAAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGCGATATTCAAGCATTTCCAATTTTAAGTCAAATAATGAACAAAATTCAAAAGCGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGATGAAATTAAACAAGCGGCAAATAAAGAAATAATTCGTCGCTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTGATTTTTCAATTAGACCTTGCATTGAAATTGCTAACAAATTAGCAAATCAATTGAATATGAATATCACAACTTTTACCTTAAACAACAATATAAATATTACAGGTAAGACATCAGCTCTTCTTGGCTCATCATCAGCTGCGTTATTAAATGCATTAAAATATTTAGCAAATATTCCTAAAGAAATCCATTTACTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGCAAAAATCCCCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAACCAATATGCAAAATTAGCCTATGATCAATTAAAAAATTTAGCAGGTATCGAAGCACATTGCAGTGCAATACTTTCTATGCAAGATATTGAAACATACAATCATTTAAATATTAACATTACCCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAG", "aa": "MNLAYEAATTDLNDKNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINITGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK", "aa_hexdigest": "ff3baa8b05c7dbe5b55584ad43b4e612", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 198618, "stop": 199745, "observations": { "mutation": [ 198894 ], "stop": [ 198894 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.5589430894308943, "identity": 0.9890000000000001, "score": 525.0, "evalue": 1.53e-184, "gene_start": 1, "gene_end": 275, "reference_start": 218, "reference_end": 492, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.9893333333333333, "score": 733.0, "evalue": 4.3e-269, "description": "Internal stop codon at 198894. Nonsense mutation around 198894.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "LMJBGEANGM_394", "locus": "GCA013368015_00368" }, { "type": "cds", "sequence": "contig_1", "start": 199799, "stop": 200362, "strand": "-", "frame": 2, "gene": "comEB", "product": "Deoxycytidylate deaminase", "db_xrefs": [ "COG:COG2131", "COG:FJ", "SO:0001217", "UniRef:UniRef50_B3PML8", "UniRef:UniRef90_D1J7T7" ], "nt": "ATGTTAAAATATTTTATTATGAATAATAAAACAAGAAAATTAATTTTTAACAACCATAAAGATAATTTAAAATGGGATATTTATTTTATGTCACTTGCCAAATTAAGTGCATTACGTTCAAAAGATCCTTTAACTAAGGTGGGAGCTTGTATTGTAAGTCCAGATAATTATGTAATATCGCTAGGATATAATGGTATGCCAACAAGTTATTTAAATAAAGAAGTTAACAATGATGATTTATTCACTTGGAATAGACCTGCGACCCAAAACGATGTCTTAAATTCCAAATATACTTACGTTGTCCATGCCGAAGCAAATGCAATAATAAATGCAAATATTACAAATTCAAAGATTATACCGGGATCTATATTATACGTAACACATTCACCTTGCTACCATTGTGCTAAGCTAATTGTCCAATCTAAAATTTCTAAAGTTGTTTATGCAGTTGCATATAAGCCTGATTCAGATGATTTTAAAGCTTCAAATAAAATTTTTGCTGCCTTCAATATTGAATGCAAAGCAATAGAAAACGATTTTGATTTAGATTTTCATATAAAATAA", "aa": "MLKYFIMNNKTRKLIFNNHKDNLKWDIYFMSLAKLSALRSKDPLTKVGACIVSPDNYVISLGYNGMPTSYLNKEVNNDDLFTWNRPATQNDVLNSKYTYVVHAEANAIINANITNSKIIPGSILYVTHSPCYHCAKLIVQSKISKVVYAVAYKPDSDDFKASNKIFAAFNIECKAIENDFDLDFHIK", "aa_hexdigest": "516ddf32e8166ced4250f49384e07404", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7T7", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9890000000000001, "score": 379.0, "evalue": 4.5e-133, "valid": true, "gene": "ComEB", "product": "Deoxycytidylate deaminase", "uniref50_id": "UniRef50_B3PML8", "cog_id": "COG2131", "cog_category": "FJ" }, "pscc": { "uniref50_id": "UniRef50_B3PML8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML8" ], "product": "Deoxycytidylate deaminase" }, "genes": [ "comEB" ], "id": "LMJBGEANGM_395", "locus": "GCA013368015_00369" }, { "type": "cds", "sequence": "contig_1", "start": 201148, "stop": 201390, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGCATCAAATATAGGTTCTCTGTTTACTGCGTTGAATTCATATACGAATTCGCCTTTTACATATGCCGGGTCTATTTTACATGAAGCGGCTACTAATCCTGGTACTCCTACTGCTAGTAAGGACCCAAGACCAAGTAATCATAATTTTGATTTAGTTTTCATTTTTATCCTTAAAATTTTAAATTTTTATATATTTGTTTTGCATATCTTTGTTGATATACAAATATCTTGTTTGTAA", "aa": "MSASNIGSLFTALNSYTNSPFTYAGSILHEAATNPGTPTASKDPRPSNHNFDLVFIFILKILNFYIFVLHIFVDIQISCL", "aa_hexdigest": "f820bc35cfcd95f3199133ad46e245d4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8839.024599999997, "isoelectric_point": 6.21491184234619 }, "id": "LMJBGEANGM_396", "locus": "GCA013368015_00370" }, { "type": "cds", "sequence": "contig_1", "start": 201523, "stop": 201696, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCATTGCCTTTTTCGTCTGTTACGGTTTTAGTTGGTTGTGGATATTCAGATTCACTTGGAATTTCATCTGAATCAAATACATATTGTTTATTAGTTCCTTTTTCAGTTAAAACAATAGCTTCTGCTAATTCTAATCTATATTTAGCAAAAGTAGGTTTTACTAAATAG", "aa": "MYSLPFSSVTVLVGCGYSDSLGISSESNTYCLLVPFSVKTIASANSNLYLAKVGFTK", "aa_hexdigest": "9e6a0de02130e57c9810135fbc313e38", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6031.877, "isoelectric_point": 7.759852409362794 }, "id": "LMJBGEANGM_397", "locus": "GCA013368015_00371" }, { "type": "cds", "sequence": "contig_1", "start": 202049, "stop": 202393, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGTAATTTAAAAGATAGAGAAGCAATTACTTTTGAATCAATTACTGGAAAAGAAAATGATGCTAACATTTCAAGCTTCTTTGAAAGTCAATTTGCATCAGGAACATATGACTTTATGCCAGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAATGGTAATGCTTTATCAAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGAAAGCTTACAATACAAAATTGGTCAATACTGA", "aa": "MFGIDANKLFEKDKFIQKVSAEGNLKDREAITFESITGKENDANISSFFESQFASGTYDFMPAPSDYIDALNAKNEQVVLKSNGNALSKSETEAYRNALNSMDKESLQYKIGQY", "aa_hexdigest": "61498998f44a5a027f596af9e671dc20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12769.975299999986, "isoelectric_point": 4.715158271789551 }, "id": "LMJBGEANGM_398", "locus": "GCA013368015_00372" }, { "type": "cds", "sequence": "contig_1", "start": 202688, "stop": 202918, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTAAATGAACGTTTGAATGTTCAAACTTCAATCTTTGGATTTGTTAGAACACCATTCATTTCTTCAAAATTAGGCAAAAATTACAAATTCAATGAAAACTTTGCTAAATTAGTATATGGATCTTCTTTAGAAGATCTTGACAGTGGAAAAGCAGACACTTCAAAATCATATCTATTTGGTAAAGGTTTGAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGA", "aa": "MKLNERLNVQTSIFGFVRTPFISSKLGKNYKFNENFAKLVYGSSLEDLDSGKADTSKSYLFGKGLSFRTLLYAAVN", "aa_hexdigest": "ac90f65eff88163342e8084987fb29e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8537.666299999997, "isoelectric_point": 9.696899223327637 }, "id": "LMJBGEANGM_399", "locus": "GCA013368015_00373" }, { "type": "cds", "sequence": "contig_1", "start": 203021, "stop": 203335, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACCATTGTAGACTTCAGAGACCAAGCTAACTCGCTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTTGGTGCTGAATTAGGTAAGGAATTAAGTCCTTCTGAAAACGCCAAAGCTTCAGAAACCGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTAAAGAAAAAATGAAAGAATTACTAGATGAATTCTTTAAAGAACAAAAAATTACCGATCCTGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATTAATGCATGA", "aa": "MNTIVDFRDQANSLFAIDKDGNKVDFGAELGKELSPSENAKASETESSISEKMKSAGFKVLKEKMKELLDEFFKEQKITDPEKQTIEFEVGFRYLNAPQLYINA", "aa_hexdigest": "600d3fa722b646bc41090aa8aeee5605", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11802.16729999999, "isoelectric_point": 4.813830757141113 }, "id": "LMJBGEANGM_400", "locus": "GCA013368015_00374" }, { "type": "cds", "sequence": "contig_1", "start": 203540, "stop": 203878, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGTTGCGCACCAAACAGGCGCTGAAAAAGATAAGTTTGAAAAAGCCTTCCCAGAAATTGCTAAATTGGCTAAAAAATTGTTAGAATATGCAAAAACAAATAAACCAAATCTATCATTGGATTTTGACGAAATTGATCAAATTGACAATAGATATTTAAATTCAAACTTACCTGCAATTTTGGCATCATATAAATTAGAAAAAGATTCTAAAACTGGAAAATATGTAATTGCTAAAAAAGATGTTAAAGGTTCAAAACCAAAACCAATCGAATTCGATTTTGGAAAAGACAGTTCAGGAAAATCAATAGAACCAACCGATCTATATCAATGA", "aa": "MVKVAHQTGAEKDKFEKAFPEIAKLAKKLLEYAKTNKPNLSLDFDEIDQIDNRYLNSNLPAILASYKLEKDSKTGKYVIAKKDVKGSKPKPIEFDFGKDSSGKSIEPTDLYQ", "aa_hexdigest": "3ca4f30a73fac45ed5af0b2470c4a899", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12625.289899999985, "isoelectric_point": 8.942103385925293 }, "id": "LMJBGEANGM_401", "locus": "GCA013368015_00375" }, { "type": "cds", "sequence": "contig_1", "start": 203984, "stop": 204082, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTTGGCCGTGATAAATTTGTTAAAACTTTAATAAACAAACACTACGTTTCACCAACTATTGCAGGATCAGCAGTTGATTCATACCAAGACTGA", "aa": "MFVGRDKFVKTLINKHYVSPTIAGSAVDSYQD", "aa_hexdigest": "0f1c52d4be80ccbd4b910d236cc6f9e8", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3588.0513, "isoelectric_point": 8.161133384704591 }, "id": "LMJBGEANGM_402", "locus": "GCA013368015_00376" }, { "type": "cds", "sequence": "contig_1", "start": 204125, "stop": 204583, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAATATATATTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTGGTTGCTGCATTTGGGAACAACCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATACTGAAATATTGCATAAACTTGAAATAGAAAACGGATTAAGATATAAAGATAACCCGGACGTTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAATAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATATTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCCGGATATAAGCGCGGAAAATGA", "aa": "MTKYILQRIAFAILTLFIISIFVYVLVAAFGNNPVNDLAQAQFQASKKSGLTYTEILHKLEIENGLRYKDNPDVLVPIIVRYGNYMKNIFTKGDFGFLYNPANNPNSALYTNMAKLFFTPLQYSLIITVPTFFISSILGIAIGVIAGYKRGK", "aa_hexdigest": "ef6b3ef7a0471528051ee3e117150061", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17072.862599999986, "isoelectric_point": 9.63056125640869 }, "id": "LMJBGEANGM_403", "locus": "GCA013368015_00377" }, { "type": "cds", "sequence": "contig_1", "start": 204590, "stop": 204979, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTCAAATGTTTTTGTATTAATATTTGTTGCTATACCATCATTTATATTGGCTCCTATTGTAATAACTATTTCGGTGGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTTGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGTTATGTAACATATACAAGAAATCAAGTTATTACAGTATTGACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCCTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGA", "aa": "MSSNVFVLIFVAIPSFILAPIVITISVALGISSVVPRPGEQSFGVMFVSYLPPIFVMSITSLAGYVTYTRNQVITVLTSNFVLIAKTKGLSSSQIFFKYVLRNISIPLFSLVFGSFIGLLSGSIIIEQY", "aa_hexdigest": "c44196720190f8b1fd87aec40f105bd6", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14021.567999999983, "isoelectric_point": 9.77445468902588 }, "id": "LMJBGEANGM_404", "locus": "GCA013368015_00378" }, { "type": "cds", "sequence": "contig_1", "start": 204802, "stop": 205149, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTAGTAGAATATTTAATCTTAGGATCTAATATTGCATATGAAACGTCAATAATAATATCGGCAATTAGCGATATAAATGTAAAGAATAATGTAGAGAACATAACAACATTAATTTCTCCTGTAGGGAAGGCATTAACTATTATTTGTGATGTTCCTGGTACTTGTCAATATTGTTCAATAATTATTGATCCTGATAATAGACCAATAAATGACCCAAAAACTAGGCTAAATAAAGGAATTGAAATATTTCTTAAAACATATTTAAAGAAAATTTGTGAAGAACTCAATCCTTTTGTTTTAGCTATTAAAACGAAATTTGATGTCAATACTGTAATAACTTGA", "aa": "MLLVEYLILGSNIAYETSIIISAISDINVKNNVENITTLISPVGKALTIICDVPGTCQYCSIIIDPDNRPINDPKTRLNKGIEIFLKTYLKKICEELNPFVLAIKTKFDVNTVIT", "aa_hexdigest": "3f3787c6c0d4cb7445f61c2608e9dc5a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12785.927199999976, "isoelectric_point": 5.326461601257324 }, "id": "LMJBGEANGM_405", "locus": "GCA013368015_00379" }, { "type": "cds", "sequence": "contig_1", "start": 205272, "stop": 205898, "strand": "+", "frame": 3, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7U0", "UniRef:UniRef90_D1J7U0" ], "nt": "ATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCGCCAGAAGATAGAATAGTAATCTCTTCTGTTGCAGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCTCCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAAGTTAATGATGTTGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATATTTAAAAATATTTAATGAACAAAAAAACTTCTATTCTCATGTCTTATTAGGAACATCATCAATAGGCTACGATATTTGA", "aa": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDVGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDI", "aa_hexdigest": "94b85e1c35d6e9f0936d175bb6a26fd4", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 205272, "stop": 206201, "observations": { "mutation": [ 205896, 205908, 205920, 206073, 206145 ], "stop": [ 205896, 205908, 205920, 206073, 206145 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U0", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 0.995, "score": 396.0, "evalue": 5.66e-136, "gene_start": 1, "gene_end": 208, "reference_start": 1, "reference_end": 208, "reference_sequence": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDIGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDIWTTAWYATWRAIKIALIVVILEAIIGISIGAFLGFYAGKLIDTIIMRVIEIFLAPPTLIWILLFVSIIGANEWSLVLTLTIVGWPGFVGITRMFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVILWIASLAFLGFFKEKKDTNLGQMLIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK" }, "paralog": false, "identity": 0.883495145631068, "score": 513.0, "evalue": 1.31e-184, "description": "Internal stop codon at 205896, 205908, 205920, 206073, 206145. Nonsense mutation around 205896, 205908, 205920, 206073, 206145.", "psc": { "uniref90_id": "UniRef90_D1J7U0", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7U0", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U0", "UniRef:UniRef50_D1J7U0", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U0" ], "product": "Oligopeptide transport system permease protein" } }, "id": "LMJBGEANGM_406", "locus": "GCA013368015_00380" }, { "type": "cds", "sequence": "contig_1", "start": 206172, "stop": 206327, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTAAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTTGTTCACGCATTACCAACTATAATTGGAAAGATTGCAAATAATATAGTAAGATCAGTACCAGGTGTTATTCTATGA", "aa": "MFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVIL", "aa_hexdigest": "766e71a14c489f6cc7701055b90c904a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5513.6307, "isoelectric_point": 10.266412925720214 }, "id": "LMJBGEANGM_407", "locus": "GCA013368015_00381" }, { "type": "cds", "sequence": "contig_1", "start": 206388, "stop": 206546, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATTGAAGCTTCACAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATCGCACTAGGTGTGCATGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAA", "aa": "MLIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK", "aa_hexdigest": "67d842025be02f62258fdb30e5d0caef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5661.721400000002, "isoelectric_point": 6.518659400939941 }, "id": "LMJBGEANGM_408", "locus": "GCA013368015_00382" }, { "type": "cds", "sequence": "contig_1", "start": 206548, "stop": 206922, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCAAAAATTATTAATAAACACGACAATTCAGACAAAAACAACTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATTTACATGTTAGTTTCCCTCAAGGTCATAAGAAAAGTATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGCGAAATAATTGGTCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACCTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGA", "aa": "MAKIINKHDNSDKNNSDTIKHQKINKSTRVLDSIRVEPLKNNQILKVRNLHVSFPQGHKKSIHIVRGVDLDVHRGEIIGLVGESGSGKSVTSKTLINVNESGIISADQIQIGEYELTNIKKQKY", "aa_hexdigest": "e9452adf205fd49c3fae600f3a1564a3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 56, "aa_cov": 0.43548387096774194, "hmm_cov": 1.0, "evalue": 1.3087781232133886e-09, "score": 34.31736755371094, "start": 66, "stop": 119 } ], "seq_stats": { "molecular_weight": 13860.667299999988, "isoelectric_point": 9.734097480773926 }, "id": "LMJBGEANGM_409", "locus": "GCA013368015_00383" }, { "type": "cds", "sequence": "contig_1", "start": 207100, "stop": 207435, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "nt": "TTGCGTTCGGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCAGGGGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATCGCTGATGAACCAACAACAGCATTAGACCCAACAGTTCAAGCTAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTACATTTATGTTATGTATGCTGGTAGAATTGTCGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGA", "aa": "MRSAEKIFYAYPHTLSGGMKQRIVITMVVALKPELIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVEKGTKQEIFTNPAHPYT", "aa_hexdigest": "aa241ded370b8c4f868d7d0398dcf46a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FD1CF63", "query_cov": 0.9099099099099099, "subject_cov": 0.9439252336448598, "identity": 0.525, "score": 119.0, "evalue": 7.69e-33, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000FD1CF63" }, "pscc": { "uniref50_id": "UniRef50_UPI000FD1CF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_410", "locus": "GCA013368015_00384" }, { "type": "cds", "sequence": "contig_1", "start": 207505, "stop": 207624, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTACGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGA", "aa": "MANLPLGDPFVYRNDYALEIDYLKEPPLIPITETHAAAT", "aa_hexdigest": "89750929c15eade9b04e273a06f3c46d", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4373.931700000001, "isoelectric_point": 4.290286827087402 }, "id": "LMJBGEANGM_411", "locus": "GCA013368015_00385" }, { "type": "cds", "sequence": "contig_1", "start": 207707, "stop": 208123, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATCTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACCGTTGGTTCAACAATACTTAGACTATATGATGATTACAATGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGCATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCTCATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAAGATATTTTTAAAGATTGA", "aa": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKD", "aa_hexdigest": "91715e0a19db33f76a755f8881eb8d8a", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9202898550724637, "subject_cov": 0.9921875, "identity": 0.701, "score": 184.0, "evalue": 1.13e-57, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_412", "locus": "GCA013368015_00386" }, { "type": "cds", "sequence": "contig_1", "start": 208352, "stop": 209521, "strand": "+", "frame": 2, "gene": "dppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_B3PML2", "UniRef:UniRef90_D1J7U2" ], "nt": "ATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGCCCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTCAATGTTTAAGCAAAACTTCAAAGATTGCTTATGAAGCTTCGCTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAGAATACATTTGCAAACTATATTCATGAATCAAAAAATGAAAAGAGTTTAATTGACATTGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAGTAAAAGGGGTCAAGTATTAGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGATATTAAAAACGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCCAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGTAGATCTAAATATTTAATTTCATATAAAGCTGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTGAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGA", "aa": "MYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMSKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFDEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKDKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHK", "aa_hexdigest": "18b95b5e3279a3214d875cfd0c2e6891", "start_type": "ATG", "rbs_motif": null, "genes": [ "dppD" ], "truncated": "both", "pseudogene": { "start": 208052, "stop": 209824, "observations": { "mutation": [ 208121, 208232, 209519, 209588 ], "stop": [ 208121, 208232, 209519, 209588 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U2", "query_cov": 1.012853470437018, "subject_cov": 0.4651711924439197, "identity": 0.98, "score": 710.0, "evalue": 2.39e-250, "gene_start": 1, "gene_end": 389, "reference_start": 216, "reference_end": 609, "reference_sequence": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKDWLNVKKNFKYTFQIEAMRLELQNYLEINKLAEPLFKKWTENLNKLEFNFDISREDNFSLFFGYLEEKQNVESIIINNMYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFNEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKANNINLKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHKWVETNLKTADAMAGVDAKHMKWVWRKRYFDSKVSYPIAKLLISSILYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQWTHNAKLEKEHKEL" }, "paralog": false, "identity": 0.9798319327731092, "score": 1126.0, "evalue": 0.0, "description": "Internal stop codon at 208121, 208232, 209519, 209588. Nonsense mutation around 208121, 208232, 209519, 209588.", "psc": { "uniref90_id": "UniRef90_D1J7U2", "gene": "DppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "uniref50_id": "UniRef50_B3PML2", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U2", "UniRef:UniRef50_B3PML2", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_B3PML2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML2" ], "product": "Oligopeptide ABC transporter ATP-binding protein" } }, "id": "LMJBGEANGM_413", "locus": "GCA013368015_00387" }, { "type": "cds", "sequence": "contig_1", "start": 209654, "stop": 210193, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "TTGTATAAAACAATAATTTATAAAGCATTGGAAGACGTTGGATTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCGCAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTTGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTCTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGTGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGA", "aa": "MYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQ", "aa_hexdigest": "f1efd8141ea362f19edc8aad725ebec4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CC3F60", "query_cov": 1.0, "subject_cov": 0.8647342995169082, "identity": 0.737, "score": 267.0, "evalue": 7.16e-89, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_414", "locus": "GCA013368015_00388" }, { "type": "cds", "sequence": "contig_1", "start": 210304, "stop": 211026, "strand": "-", "frame": 3, "gene": "rsuA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG1187", "COG:J", "RefSeq:WP_113239996.1", "SO:0001217", "UniParc:UPI000DDEBEA4", "UniRef:UniRef100_A0A6A8PYQ2", "UniRef:UniRef50_D1J7U3", "UniRef:UniRef90_D1J7U3" ], "nt": "ATGGAAGAAAAAGCAAAACGAATTCAAAAAATTTTAAGTGAGCAAGGCATTTGTTCTAGAAGAAAAGCAGAAGAATTAATTTTATCGGGGAAGATTTTAATAAATAATAAAATTGCAAAATTAGGCGAATGTGTAACTATAAATGACGAAATTAAAATAAACAATAGAATTATCAAATTAAATAATAATGTTGCAAATAATTTTAAATACATTGTTTTAAATAAACCCATGAATACAATTTGTACAGCAAGTGATCCTCAAAATAGAAAAACAATTTACGAATATCTTAAAGCAAAAGAACGCTTATTTTCAATAGGAAGATTAGACTTTAATACAACAGGAATTATATTAATCACAAACGATGGAGAATTTGCTAATTTATTAAGCCATCCTTCTTCTGAAATTCCTAGAATTTATATTGCTACATTAGAACGTGAAATGCTTGATGGCGAATTAAACTTATTAAATTCAAATCACGTATTATTAAATGGCAAAATTTCAAAACAAAAAGTTACAAAATTAAGTGCTTTTAAATATGAAGTTCTACTATATGAAGGCAGAAACCATCACGTTAAAAATCTATTTTTGTTAGTAAATAATTATGTTAAAAAATTACATAGAAAATCTTTTGGATGCATTGATGATAGTAATTTAAAAATAGGTCAATATAGAGATTTAACCTCAAGCGAAATTAATAAATTATTAGCTTATATAAAGAAATAA", "aa": "MEEKAKRIQKILSEQGICSRRKAEELILSGKILINNKIAKLGECVTINDEIKINNRIIKLNNNVANNFKYIVLNKPMNTICTASDPQNRKTIYEYLKAKERLFSIGRLDFNTTGIILITNDGEFANLLSHPSSEIPRIYIATLEREMLDGELNLLNSNHVLLNGKISKQKVTKLSAFKYEVLLYEGRNHHVKNLFLLVNNYVKKLHRKSFGCIDDSNLKIGQYRDLTSSEINKLLAYIKK", "aa_hexdigest": "63f487553b7ff0c036ffd94c3bc5a4c5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000DDEBEA4", "ncbi_nrp_id": "WP_113239996.1", "uniref100_id": "UniRef100_A0A6A8PYQ2", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DDEBEA4", "RefSeq:WP_113239996.1", "UniRef:UniRef100_A0A6A8PYQ2" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PYQ2", "uniref90_id": "UniRef90_D1J7U3" }, "psc": { "uniref90_id": "UniRef90_D1J7U3", "gene": "RsuA", "product": "Pseudouridine synthase", "uniref50_id": "UniRef50_D1J7U3", "cog_id": "COG1187", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U3" ], "product": "Pseudouridine synthase" }, "genes": [ "rsuA" ], "id": "LMJBGEANGM_415", "locus": "GCA013368015_00389" }, { "type": "cds", "sequence": "contig_1", "start": 211134, "stop": 211388, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4", "UniRef:UniRef90_D1J7U4" ], "nt": "ATGGAATATTTACAAAATATAGACTTAAGCAAGATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGTTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGA", "aa": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESE", "aa_hexdigest": "cc38cd88563039e12c5649d4cb46963b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 211134, "stop": 211472, "observations": { "mutation": [ 211386, 211401 ], "stop": [ 211386, 211401 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U4", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 162.0, "evalue": 1.84e-50, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESEWYLAYWSKNAYYKKLNYIVNLFIDFINQ" }, "paralog": false, "identity": 0.9821428571428571, "score": 221.0, "evalue": 3.5e-76, "description": "Internal stop codon at 211386, 211401. Nonsense mutation around 211386, 211401.", "psc": { "uniref90_id": "UniRef90_D1J7U4", "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U4", "UniRef:UniRef50_D1J7U4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10245.543599999992, "isoelectric_point": 5.506072807312011 }, "id": "LMJBGEANGM_416", "locus": "GCA013368015_00390" }, { "type": "cds", "sequence": "contig_1", "start": 211652, "stop": 211954, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGATAAACATGAATATACATATACTCTTATTTCTGAATTTAATGAGGCAACAAAAACTAAAAAAGTTCAGGATTTAAAAAATATTAATTTTGAAATTTTTTATAAGGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAGATATATAGCAATAACAATTCAAACAGAGAACTAGTTTCAAATAAAAATGGCGTTGAATATGATTTATGA", "aa": "MNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDLKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNRELVSNKNGVEYDL", "aa_hexdigest": "6d2105b9fb6924d4e6ab427b3da18e82", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11979.218999999992, "isoelectric_point": 4.950130653381346 }, "id": "LMJBGEANGM_417", "locus": "GCA013368015_00391" }, { "type": "cds", "sequence": "contig_1", "start": 212003, "stop": 212536, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8", "UniRef:UniRef90_A0A454C9K8" ], "nt": "ATGGAACATTATTCATTTGTTGAACAAGTTAAATACAAATTTATGCCAACTACTCTAATGTTGGGAAAACATAATAAAAAATTATTTGATGTTGAAATATCAAAAAAAGAAATTTCTAACAAAAGTGGCATAATAAGCGATTTTTCTCAACTAATTCAAAGCGATATTAATGGTTTTAATGTATCTGTTGATATTGATAAACAATTTATAAAAGGCAACTTGAATGCAAAAATTAATGATTTCAGTAAAAATGAGCATAAGCAAATTTCAATAGATTCTTATTCATATTATGACAAGGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGCGCAGCTAAAGGCTTATTGTTGCCTCTTAATTATTCAGGAAATTATTCTTATTTACTAAATATTAATTTTGGTGAGGATTTGAAAGGTTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAGTAAAATTAAATGTAAATAATATATTTAAACAACAAAATTTCATAAAATGA", "aa": "MEHYSFVEQVKYKFMPTTLMLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSVDIDKQFIKGNLNAKINDFSKNEHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIK", "aa_hexdigest": "634f4935197db6d320e2f315653948bd", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 211703, "stop": 212617, "observations": { "mutation": [ 211952, 212534 ], "stop": [ 211952, 212534 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9K8", "query_cov": 1.0, "subject_cov": 0.4645669291338583, "identity": 0.9890000000000001, "score": 340.0, "evalue": 4.04e-115, "gene_start": 1, "gene_end": 177, "reference_start": 178, "reference_end": 354, "reference_sequence": "MFPIVMQAINDQEPTKWIENINAYTTKQLYLDKWTNSFQKVDFKLQRLIENNKFIIEFKYLNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNRELVSNKNGVEYDLWTKLRILSLADRDIPTDMEHYSFVEQVKYKFMPTTLVLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFSKNEHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIKWSVVKAEDIRSLINNSNTLEELMKGGK" }, "paralog": false, "identity": 0.9835526315789473, "score": 591.0, "evalue": 1.6e-216, "description": "Internal stop codon at 211952, 212534. Nonsense mutation around 211952, 212534.", "psc": { "uniref90_id": "UniRef90_A0A454C9K8", "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9K8", "UniRef:UniRef50_A0A454C9K8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20313.9557, "isoelectric_point": 9.268120002746581 }, "id": "LMJBGEANGM_418", "locus": "GCA013368015_00392" }, { "type": "cds", "sequence": "contig_1", "start": 212617, "stop": 212967, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6", "UniRef:UniRef90_D1J7U6" ], "nt": "ATGTTGATTGCAAAAAAGAAAAAAATAATTTTCGGTTTATCTCTTGGATTAGCTTGTGCCGTTGGCGCTGGCGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACATCAATGACAACAAAAACATTAATTCTGGCAATGAAAACGAAAAAGATAACACTATTGATGGCCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGCCAAGCGAATGATGATGATAAAAAGCTTAAGCCAGAAGCAAAGCCAAGGCCGAATATAAAAACAGAGATTAATTGA", "aa": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNINDNKNINSGNENEKDNTIDGQNEDSKIEDSNEDGQANDDDKKLKPEAKPRPNIKTEIN", "aa_hexdigest": "73671fd3ed3ea9c4490f512567d724f2", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 212617, "stop": 213222, "observations": { "mutation": [ 212965, 213163 ], "stop": [ 212965, 213163 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U6", "query_cov": 1.0, "subject_cov": 0.5771144278606966, "identity": 0.888, "score": 130.0, "evalue": 7.99e-36, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNNSDNKNINSGNENEKDNTIDNQNEDSKIEDSNEDGQANDDDKKLKPEVKPRPNIKTEINWREIFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGDVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.9203980099502488, "score": 308.0, "evalue": 1.09e-108, "description": "Internal stop codon at 212965, 213163. Nonsense mutation around 212965, 213163.", "psc": { "uniref90_id": "UniRef90_D1J7U6", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U6", "UniRef:UniRef50_D1J7U6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6" ], "product": "Lipoprotein" } }, "id": "LMJBGEANGM_419", "locus": "GCA013368015_00393" }, { "type": "cds", "sequence": "contig_1", "start": 213052, "stop": 213165, "strand": "+", "frame": 1, "gene": null, "product": "MHO_1590 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0", "UniRef:UniRef90_UPI0035A6F3B0" ], "nt": "ATGATTACTTATATAATAAAGGATATTTTAAATAGGATGACTATTTTAGATGGCAATGTTTCTTATGCTTATAAACAAGAGGATGATCAAAATTTATATATTGCTTTTAAATGA", "aa": "MITYIIKDILNRMTILDGNVSYAYKQEDDQNLYIAFK", "aa_hexdigest": "fbfa7e545dfa804fbe96fcddc547dae2", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 212974, "stop": 213222, "observations": { "mutation": [ 213163 ], "stop": [ 213163 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "query_cov": 1.0, "subject_cov": 0.45121951219512196, "identity": 1.0, "score": 77.8, "evalue": 5.11e-18, "gene_start": 1, "gene_end": 37, "reference_start": 27, "reference_end": 63, "reference_sequence": "MFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGNVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.975609756097561, "score": 164.0, "evalue": 1.53e-54, "description": "Internal stop codon at 213163. Nonsense mutation around 213163.", "psc": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "product": "MHO_1590 family protein", "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0035A6F3B0", "UniRef:UniRef50_UPI0035A6F3B0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0" ], "product": "MHO_1590 family protein" } }, "id": "LMJBGEANGM_420", "locus": "GCA013368015_00394" }, { "type": "cds", "sequence": "contig_1", "start": 213231, "stop": 213455, "strand": "+", "frame": 3, "gene": "acpP", "product": "Acyl carrier protein", "db_xrefs": [ "COG:COG0236", "COG:I", "RefSeq:WP_020002664.1", "SO:0001217", "UniParc:UPI0002DDE617", "UniRef:UniRef100_A0A2K9YT00", "UniRef:UniRef50_A0AA43R1D2", "UniRef:UniRef90_A0A2K9YT00" ], "nt": "ATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAATTTAATAAAGAAACTTTTATTCGTGATTTAAATATTGATAGTTTAGATTTGGTTATACTTGTTAGTGATTTAGAAGAGGCTTACAATATAAAAATAAGTGATGAAGAATTGCTTTCATTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAA", "aa": "MESEKIIINAIKKLTKTKFNKETFIRDLNIDSLDLVILVSDLEEAYNIKISDEELLSLKTINDIIELFDKKTNK", "aa_hexdigest": "0fda9036b020adeab1537197b28e01cc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002DDE617", "ncbi_nrp_id": "WP_020002664.1", "uniref100_id": "UniRef100_A0A2K9YT00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DDE617", "RefSeq:WP_020002664.1", "UniRef:UniRef100_A0A2K9YT00" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT00", "uniref90_id": "UniRef90_A0A2K9YT00" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YT00", "gene": "AcpP", "product": "Acyl carrier protein", "uniref50_id": "UniRef50_A0AA43R1D2", "cog_id": "COG0236", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_A0AA43R1D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43R1D2" ], "product": "Acyl carrier protein" }, "genes": [ "acpP" ], "id": "LMJBGEANGM_421", "locus": "GCA013368015_00395" }, { "type": "tRNA", "sequence": "contig_1", "start": 213488, "stop": 213563, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 66.8, "nt": "ATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCA", "db_xrefs": [], "id": "LMJBGEANGM_15", "locus": "GCA013368015_00396" }, { "type": "tRNA", "sequence": "contig_1", "start": 213669, "stop": 213743, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 76.0, "nt": "GCCGTCATAGCTCAGGGGCAGAGCACTTCCATGGTAAGGAAGGGGTCGTTGGTTCAATTCCAATTGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 213709, 213711 ], "id": "LMJBGEANGM_32", "locus": "GCA013368015_00397" }, { "type": "cds", "sequence": "contig_1", "start": 213853, "stop": 214932, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0006415", "GO:0016149", "KEGG:K02835", "RefSeq:WP_020002663.1", "SO:0001217", "UniParc:UPI0003822901", "UniRef:UniRef100_A0A2K9YT01", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_D1J7U7" ], "nt": "ATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATACGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAGATTTATGATGCTTTTACAAAATATCAAAACATTGAAGAACTATATTTCTTGTCAAAAGAAATTTTAGCAAATGAAAAAGATCAAGAATTAATTGAGCTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGCGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATTAAAGGTTTTAAAATCAAGATAATTGATTCTACATATGGCGCAGCCGGAGGATATAGCCAAATTGTTTTTAGTATAAAAGGCGAAAAAGTTTTTTCACAACTAAAATTTGAACGTGGTGTACATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGACGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCCGAAGTTGATGAAGACGTAAAAATCGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGCTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCGACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCCTTAAAAGTTTTAAAAAGTAGATTGTATGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAACTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTATCCTCAAGATAGAATTACAGATCATAGAATTGGCTTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATCATTGAAGCAGGAAATTAA", "aa": "MEKTMYDSLLSIKNQYDELTEKLNDPNIYNDIKAYTRIAKEKSELQEIYDAFTKYQNIEELYFLSKEILANEKDQELIELAKNDIDNQMNQLQEIEEEIKELLLPQDENDKRDVIMEIRGAAGGDEANIFSGDLFKMYQKYAEIKGFKIKIIDSTYGAAGGYSQIVFSIKGEKVFSQLKFERGVHRVQRVPATETQGRVHTSTATVTVLPEVDEDVKIEIRPEDIEVNVFRSSGAGGQSVNTTDSAVRITHKPTGLVVTSQDERSQIQNKETALKVLKSRLYELELRKREEAESGLRKLAGTGDRSEKIRTYNYPQDRITDHRIGFSTSLKVVMEGELDKIIEALIADEKAKKIIEAGN", "aa_hexdigest": "c3c8ccaa135d7fe80bd1b50f2783a8ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0003822901", "ncbi_nrp_id": "WP_020002663.1", "uniref100_id": "UniRef100_A0A2K9YT01", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003822901", "RefSeq:WP_020002663.1", "UniRef:UniRef100_A0A2K9YT01" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT01", "uniref90_id": "UniRef90_D1J7U7" }, "psc": { "uniref90_id": "UniRef90_D1J7U7", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "LMJBGEANGM_422", "locus": "GCA013368015_00398" }, { "type": "cds", "sequence": "contig_1", "start": 214932, "stop": 215324, "strand": "+", "frame": 3, "gene": null, "product": "methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "nt": "ATGATAGATAAAGAAATTCTACTACGCGAAAAAGAAAGATATAATTTACCCCTTTATATTTCAAAAAAAGAATTAAGAATGCTTAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTGAATTTAAAATATAAAACCTTGATTCCTAGATACGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTAGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGACGCTAATGTTACATTAAGTGATATTAGCCGTTTTGCAATCAAACAAACAAAAATAAATGCCAAATTAAATAATTTAAATGTAAATGTAATTCGTTCAAATTGA", "aa": "MIDKEILLREKERYNLPLYISKKELRMLKKDVPVQKIIGYQEFQNVILNLKYKTLIPRYETEEVILAAYPFIKKESKVLDLGCGSGLIGLAIKKNKDANVTLSDISRFAIKQTKINAKLNNLNVNVIRSN", "aa_hexdigest": "fb92bb821032a6ab69fada4c0e251a7f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0022080B54", "query_cov": 0.8846153846153846, "subject_cov": 0.9274193548387096, "identity": 0.513, "score": 94.4, "evalue": 1.82e-22, "valid": false, "product": "methyltransferase domain-containing protein", "uniref50_id": "UniRef50_UPI0022080B54" }, "pscc": { "uniref50_id": "UniRef50_UPI0022080B54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "product": "methyltransferase domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_423", "locus": "GCA013368015_00399" }, { "type": "cds", "sequence": "contig_1", "start": 215430, "stop": 215639, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCATTTAACTATTTAAATGAAAAAGGAATAATTATTTTTGAAGTCGACCCTTATTGCTCTTTATGGTTTAAAAATAAATTTCCAAATGTACTAATATTAAAAGATATAAACAATAAAGATAGAATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAG", "aa": "MFARDNGLFAYKKILKSAFNYLNEKGIIIFEVDPYCSLWFKNKFPNVLILKDINNKDRIALLAKNDLKN", "aa_hexdigest": "e99f8748d453c079e19efaab39262372", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8112.4699999999975, "isoelectric_point": 9.519095420837402 }, "id": "LMJBGEANGM_424", "locus": "GCA013368015_00400" }, { "type": "cds", "sequence": "contig_1", "start": 215674, "stop": 216786, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408", "SO:0001217", "UniRef:UniRef50_A0A448ZV77", "UniRef:UniRef90_D1J7U9" ], "nt": "ATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATACCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATATGAAGTTTTATCTGACCCTGAAAAAAGACAAAAATATGATAAGTTCGGACATTCTGCATTCGACCAAAGTTCATTCGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCGAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGTTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTGTAGATTCAATATTTGGAAAGAATGTAACTGTTCAATTAAAGAAATATAGCGAATGTTCACATTGTCATGGTTCAAGAGCGGAAAGCGATAGTGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTCTTTTCAACAATTAGTCCTTGCAACAAATGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGATATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGCGGAATTAATAATGGCGAATATATTAAATTGTCAGGATATGGTAATCCTTCTCGCAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACCATTAATGTTCCTACACCATACGGAAATAAAGATATAAAATTAAATAAAAACATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATTACATTAAAACCTTATATTCCTTCGCTTAACAAAGAAGATAACAAAAAAGTTCAAGAAATATTTAGCAACTATAAAGATGTCGAATTTCAAAAATGA", "aa": "MENKRDYYEVLGISKNATEKEIKSAYRKLAMQYHPDRNKAPDAEEKFKEVSEAYEVLSDPEKRQKYDKFGHSAFDQSSFGYSEDVFNSFFNSFRDMFSGGGFEVDSSDDSDDPFSSIFGFGSNRSRKTKGSDLQMKLSIDFVDSIFGKNVTVQLKKYSECSHCHGSRAESDSDIVSCPQCHGSGQVQQKMGFFSTISPCNKCGGLGKSIKNTCHVCSGKGYEYKLVNENIQIPGGINNGEYIKLSGYGNPSRNGGSNGDLYIIIAIRPHKFYERFNNDIVVSMPISVFSIINEDTINVPTPYGNKDIKLNKNMKLDGVITLDGYGFPIKNSSKKGNFIITLKPYIPSLNKEDNKKVQEIFSNYKDVEFQK", "aa_hexdigest": "0d17e4dae60fd352e2d4bfec96dc2669", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7U9", "query_cov": 1.0, "subject_cov": 0.9814323607427056, "identity": 0.997, "score": 734.0, "evalue": 1.13e-267, "valid": true, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_A0A448ZV77", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZV77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZV77" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "LMJBGEANGM_425", "locus": "GCA013368015_00401" }, { "type": "cds", "sequence": "contig_1", "start": 216975, "stop": 217130, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAATTATGATTGTTGAATCGCCAAATAAAGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAGGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATTGGAGGATTAGGTATAGATTTTGAAAATTGA", "aa": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFEN", "aa_hexdigest": "5944c78f065504df67fff7c2f6f5aca3", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5484.369399999998, "isoelectric_point": 6.527242088317871 }, "id": "LMJBGEANGM_426", "locus": "GCA013368015_00402" }, { "type": "cds", "sequence": "contig_1", "start": 217140, "stop": 217643, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "nt": "ATGGTAATTGATCCAAGTAAGAGAAAAATAGTATCCGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGCGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAAATAAATATAAAAGAGTGAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGATAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATTGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTATTCCAACATTATATTCAAAAATTGAAGCACATACTGCTGATGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGTGAAAATGAATGA", "aa": "MVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQNKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFIPTLYSKIEAHTADEQIANFVYRDNLDFVGENE", "aa_hexdigest": "ddea5bb7928be15dc897d2615a93a2c3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF0FCFB", "query_cov": 0.8203592814371258, "subject_cov": 0.8726114649681529, "identity": 0.555, "score": 140.0, "evalue": 1.74e-39, "valid": false, "product": "DNA topoisomerase", "uniref50_id": "UniRef50_UPI002FF05F56" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF05F56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "product": "DNA topoisomerase" }, "genes": [], "id": "LMJBGEANGM_427", "locus": "GCA013368015_00403" }, { "type": "cds", "sequence": "contig_1", "start": 217698, "stop": 218582, "strand": "+", "frame": 3, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0550", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_B3PMD8", "UniRef:UniRef90_D1J7V0" ], "nt": "TTGCAGGTAATAGACTTTAAAAAATCTATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTGTATAAAGAATCTCAATATAGTTCGGCAACGGTTCAAATTGCTGCACAAAAACTATTTGAAATGGGGTTAATTTCTTATCCCAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCTCAAAAATATGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTGCTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACACCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTTAAAATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTATTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTAGAATTTGTTAAATTAGACAAAGAAACTAAGGCTCCTGCAAGATATAATGATGGTTCATTAATTAAGATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACGACAGTTAAAATTATAAAAGATAGACTATTTGTTGAAACTAAAAATAATAGTTTATATCCTACAGATTATGGAAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATTAATGAAGAATATACAGCTAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGGTTATCAGATTATAAAGTTTTATTGAAAGATTTTTGA", "aa": "MQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKVLLKDF", "aa_hexdigest": "5576ba88f2d2f738e869cd96c76c9f45", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 217398, "stop": 218826, "observations": { "insertion": [ 218787 ], "mutation": [ 217641, 218580 ], "stop": [ 217641, 218580 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V0", "query_cov": 1.0, "subject_cov": 0.4772727272727273, "identity": 0.997, "score": 569.0, "evalue": 7.74e-200, "gene_start": 1, "gene_end": 294, "reference_start": 242, "reference_end": 535, "reference_sequence": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFENWEPKMVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFVPTLYSKIEAHTADEQIANFVYRDNLDFVGENEWIKREKVDDILAQLNQNKSLQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKVLLKDFWNNFNLQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFLKEDIINNFYF" }, "paralog": false, "identity": 0.991578947368421, "score": 921.0, "evalue": 0.0, "description": "Internal stop codon at 217641, 218580. Frameshift due to insertion around 218787. Nonsense mutation around 217641, 218580.", "psc": { "uniref90_id": "UniRef90_D1J7V0", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_B3PMD8", "cog_id": "COG0550", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V0", "EC:5.6.2.1", "UniRef:UniRef50_B3PMD8", "COG:COG0550", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMD8" ], "product": "DNA topoisomerase 1" } }, "id": "LMJBGEANGM_428", "locus": "GCA013368015_00404" }, { "type": "cds", "sequence": "contig_1", "start": 218595, "stop": 218813, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01396.24" ], "nt": "TTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTCTTGCCTCAAGAAATTTTGCAAGAAAAATGTCCTCTTTGTGGACACGACTTACTTTATAGATATACTAAATCAAAACACCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATTGACAATGAAAAGCCTAAATTTTTTAAAAGAAGATACAATAAATAA", "aa": "MQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFFKRRYNK", "aa_hexdigest": "e7c9e3d77905d61d1e4033d4c4c50f55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01396.24", "name": "Zn_ribbon_Top1", "length": 40, "aa_cov": 0.5555555555555556, "hmm_cov": 0.95, "evalue": 9.658570071358836e-17, "score": 56.109832763671875, "start": 21, "stop": 60 } ], "seq_stats": { "molecular_weight": 8776.033999999992, "isoelectric_point": 9.182634925842287 }, "id": "LMJBGEANGM_429", "locus": "GCA013368015_00405" }, { "type": "cds", "sequence": "contig_1", "start": 219135, "stop": 219278, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGTTTACAATATTGATATTTATTCAAAAACCGGAAGAGCAGATGCTAACGATTTAGACTTAAGAAATAAAGACAAAATTTTTAAGGGCTATAAAGATGTTACATTTGAATTCAGCACCGAAAATGTTCCAGGTAGATGA", "aa": "MNVYNIDIYSKTGRADANDLDLRNKDKIFKGYKDVTFEFSTENVPGR", "aa_hexdigest": "dd854a1456e28451b17b8a780506a92e", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5460.008500000001, "isoelectric_point": 6.071166038513183 }, "id": "LMJBGEANGM_430", "locus": "GCA013368015_00406" }, { "type": "cds", "sequence": "contig_1", "start": 219402, "stop": 220106, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158CC148", "UniRef:UniRef90_UPI00158CC148" ], "nt": "TTGCAAAGTTTTATTAATAAAGATTTGAAGGATCAAAAATATAATTCAATTAGAACAAAAATTGAAGATAAAATAAAAGGTGTTTCAAGCATAAATAAAAATTCAAAAATTCAAGATATAGAAAATGCTAAAACTAAATTAGATTCTGCTAAAAACGAGTTTGGCAGAATAATAAATAATATTAGAGAATTCAATTCCCAAAAACAAAAAACTGAAGAAAAGTTAAAATTTTTAGATACTGAAAACATTGGCACCTCAGGTAGTCTTTCAAACAAAAATAAAAATATAAAGACAATTTTCCAAGGCTTCATAACCGAAATGGAAAAAGAATTAATTAATAAAAATGCTGACAATAAAGCAAAAGATGAATGCATTAACAAAATAAACTTTTTAGACAAGGCTTTAATTTCAAGATATGAAGGAATTTTGAATGCTGCATATAAATTTTTCGATGATACATCAACACAAAATAACCAAACAGAAAAAAATGAATTTTTTGATAATTTAAGCAATAATTTAGTTGATCAAAAAGTGTTTGATCAATTAGAAAAAATGAATATGCCTCATTTTGAAGAATTTTATAATACATTCTACAACAAAGGTAATGCGCCATTAAAACCTGATGGTAATCCAAATTATAACGATCCAAAATATCTTGAAGAAATTAGAAAACAAAATGAATTAGAAGGGCCTGCTGTAAAGTGA", "aa": "MQSFINKDLKDQKYNSIRTKIEDKIKGVSSINKNSKIQDIENAKTKLDSAKNEFGRIINNIREFNSQKQKTEEKLKFLDTENIGTSGSLSNKNKNIKTIFQGFITEMEKELINKNADNKAKDECINKINFLDKALISRYEGILNAAYKFFDDTSTQNNQTEKNEFFDNLSNNLVDQKVFDQLEKMNMPHFEEFYNTFYNKGNAPLKPDGNPNYNDPKYLEEIRKQNELEGPAVK", "aa_hexdigest": "1e219fa6b8f765df3f492c09a4180a90", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 219099, "stop": 220106, "observations": { "mutation": [ 219137, 219404 ], "stop": [ 219137, 219404 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158CC148", "query_cov": 1.0, "subject_cov": 0.5894206549118388, "identity": 1.0, "score": 447.0, "evalue": 3.97e-156, "gene_start": 1, "gene_end": 234, "reference_start": 1, "reference_end": 234, "reference_sequence": "MQSFINKDLKDQKYNSIRTKIEDKIKGVSSINKNSKIQDIENAKTKLDSAKNEFGRIINNIREFNSQKQKTEEKLKFLDTENIGTSGSLSNKNKNIKTIFQGFITEMEKELINKNADNKAKDECINKINFLDKALISRYEGILNAAYKFFDDTSTQNNQTEKNEFFDNLSNNLVDQKVFDQLEKMNMPHFEEFYNTFYNKGNAPLKPDGNPNYNDPKYLEEIRKQNELEGPAVKWSAFDDILENAFVRTLQTSFIKKCENLFSHKTEEYYGTINRKLNVYNIDIYSKTGRADANDLDLRNKDKIFKGYKDVTFEFSTENVPGRWQKNFKGLAKWDGAKNRYRFVGIVSIYSNYEYNLVISTKGENYKKTFSWEYKLGSNDKGQNYNPIIIDRISGIN" }, "paralog": false, "identity": 0.991044776119403, "score": 670.0, "evalue": 2.36e-246, "description": "Internal stop codon at 219137, 219404. Nonsense mutation around 219137, 219404.", "psc": { "uniref90_id": "UniRef90_UPI00158CC148", "uniref50_id": "UniRef50_UPI00158CC148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158CC148", "UniRef:UniRef50_UPI00158CC148" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00158CC148", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158CC148" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 27208.156500000012, "isoelectric_point": 7.650380516052247 }, "id": "LMJBGEANGM_431", "locus": "GCA013368015_00407" }, { "type": "cds", "sequence": "contig_1", "start": 220155, "stop": 225959, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936686.1", "SO:0001217", "UniParc:UPI00158C9899", "UniRef:UniRef100_UPI00158C9899", "UniRef:UniRef50_A0A454CA13", "UniRef:UniRef90_A0A454CA13", "PFAM:PF04778.17" ], "nt": "ATGAAAAAAAGAATAAACATTTTAATGTCAATTTCAATCGTTTGTGCTGCTGCATCAACGTCAGCTATTGCTGCTATGTGTTCAATAAGCAATAACTCCACGAAAAAGTATAACGAAGCAAAATCTAGATTACTTAATCTAATTAAGAAACTAGATAATGAAGGTCAAAAGAAAGCAAATGATTTTATTGCAAGACAAGATAAAAAATTTAATAGTACAGCATTTAAAAATCATTCAAACACATCAAAACTTATTGATGAAATAGAAATTTTTAGTAAAAAAATATTAGAAAATCTACAAAAAGATGAAAAACAAAGATTAGAAGAATTAAATAAATTAAATAAATTAAGATTAGATTTACAAAACTTAATTAATTCAAATGACGGTCAAAACGTCGATTCTTCTGATGCTAAAAAAGCATTAAATGAAAATCAAATAGATGATTCTTTGCCAATAGATAAAATTAAAAAGACAAAGGAAAATCTTGAAAATGCAAAAAAAGAACTTCTAAATAAAATAAATACTGAAAAAGAATTACAATCAAAAATTTTTAATGATAAGAAACAAGAATTAAAAAGAGTTCTTGATTTAGAAGATGCAAAAGAAGTAGATTCTATAAAAGAACAAAAAGTTTATATCGAAACAAATATAAGTGAAACATCATCAATTGAAAATATAAAAAACAAAATTATTGAAGTTGAAAAAGCAACTTCTTCTTTAACATCAAAAATTTTAAATACCAAACAACAAGAACTTCAAGAATTTGAAAATATTAAAAAAGACTTACAAGATTTTATTAACACAAAATTAAATGATGCAAAATATCAATCAATAAAACAAAAGGCTTTAGATAAAATAAATAGTTTAAATGGAATAAATAAAAATTCAACAATTAAAGAAATAAAAGCTGGACAAAATGCTTTAATAAAGGCAAAAGAAGAAGCAGGCCTTGAAAAAGAAAAATTAGATGGTCAAAATATAAAAGATACTCTTAAAGAAACTATAAATAATGCCAAAGAATTTAAAAAGTTATTAATTGACAATGACCAAAAAATAGTTGATTTAAAATCTAATTTAGATAATGAAATTTCTAAAGCAGAACAATCTCTTTCAAAAGACAAAGAATCAATGGAATCAGCCAATGATTTATTGAATACTAAACTTATTGAATATAAAGAAATCTTAAATAAATTCAATCAAGAAAAAGAAGTAAAATTCAATGAATTAGAACAAACAAGAAAGAATATTGACAATTTCTTAACAGATGACGTTAAAAATAATCCAAATTATGCAACTTTAGTTAAAGATTTAACCAATGCAAAAGATGATAAGAAATCAGTAACTAAATCTTCAAATAAATCAGACATTATTGCTGCAAATGAAGCATTAATACAAGCATTAGCAGATGCTAATAAAGCAAAAGATCAAGTTGATGAAGCAAATAAATCAATTAAAGAACAATTGAATGCATTAATTGACAAAGCAAACACATTATTGCCTCAATTAAATGATAATGATAGTGAAATTGTAAAAGCTAAAGAATCATTAAATGCAGAAATTACCAATGCAAATAAAGCAGTTAACCAAAATGACAATGCTTCGATGCAATCTGCTAAATCTTCATTAGATGACAAAGTTACTAAAATTCAAAATCAATTAACTGAGTTCAATAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAGCAAGAAAGAATATTGACAATTTCTTAACAGATGACGTTAAAAATAATCCAAATTATGCAACTTTAGTTAAGAATTTAACCAATGCAAAAGATGCTAAAAAATCAGTAACTAAATCTTCAAATAAATCAGACATTATTGCTGCAAATGAAGCATTAAAACAAGCCCTTGATAAGGCAAAAGTAGCAAAAGAACAAATTGATGATGCAAATAAATCAATTAAAGAACAATTAAGCGATTCAATTGACAATGCAAAACAATTATTAAATAAATTAATAGATTCAGACAAAGACATTCAAAAAGCAAAAACTGAATTAAATCAAGAAATTCAAAGTGCAAATCAAGCGCTTAATTTAAATAACCCAACATCAATGCAATCAGCAAAAGAATCTCTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACATTTAATAAAGATAAAGATGCTAAATTTAAAGAATTAGAAAAAACAAGAAAAGACATTGATGAATTTATTAATTCAAATAAAAATAATTCAACTTATTCAAACATAATTTCAAAATTAACCACGACTAAAAATGAAAAAAATTCAGTTTCTGAATCTTCAAATAAATTGGAAATTGAAGACGCTAATACTTTGCTAAAAAATTCTTTAAAAGAAGCAAATGATAAAAAACAAGAAATTGATAAATTTGTTTCAGCTAAAAAAGAATTAGAAGAATTTATTGAAAATGATGAAGATACTAAAAGTGATGAATTGGTTTCAAATTTAAACGATGCTAAACAAGCTTTAGCAAAATTCAGTAGCATAGATGATTCACACGAAATCACTGAAATTAAAAAAGCTACTGATGATTTAACAACTGCAAAAACAAAATTAAAAGAAGAAATTAAAAACAAAAAACAAGAGTTGTTTAATGCATTTGAATCATCTAAAACAGAATTGCAAAACTACATTACAAACGATATTAAAGATTCAAAATATAAAATTATTCAAGATAAATTTAATAATGTTATTGATGAATATAAAAACATTACATCAAACGACAAAATTTCAAAAATTAAAGAAGCTACAAATAATTTAAATGCAAAATTTACAGAAATTGGAAGCAACAAGGCAATATTAGATAAGTTTTATGAATTGAAGTCAAAACTTGAAGATTTATTAAAAACTGCAAATCTTAAAGATGATGCAAAATCCATAAATATTGATTTATCTTCTGAAGATCAAGCTTTTGCTGACGCTAAGGCCATAAACAATAATTCAACTATTGAAAATATTGAACAAGTTAACCAAAAATTACAAACAGCAATAGACAAATTAGCTGAAGATATTAAAAAAATAAAAGAAGATAAAAAAGCTAAATTCGGAGAATTTAAAACAGAAAAAGAACATCTAGAAAACATAATCAAAACCAAAGGTTTAGATAAATTTATTAATATCGATGATGAAATAAAATCAATAAAAAAACAAATTGATGAAGAAATTAAAAAACACAAAATAAACGAAAATTCAAAAATTTCTGATATTTTAGCAGCAAAAAATAGTATCGATGAAACACAAAGAAAACTATTAGGTTCGTTGGATAATTATTCAACTCAAAGAACAGAATCTATAGACAATTACAATAAGCAAAAAACCGAATTAAATTCTTTGACAAATTCTAATAATTTTAAACAATTAGAAGAATCTAAAAAAACTAAATTCAATAATAAAAAACAAGAATATGAAGTCTTGAATTTAGAAACATTAAGTAAGAATAAAATTCAAGAAAAATCACAAGAAATTAAAAAATTAATAGATAATATAAATAAAGAAATCTCAGATGCTAAAAAACAAGAATATTCAAAATTTGATAAAGCAAAAAAAGAATTAGAAAAATACATTAATGATGAATTAAATAATCAAAAATACGATTCAATTAAAAAAGAAGCGCAAAATAAGATAGATAGTTTTAGTACAATAAAAGAAGAATCTTTAACAACTAAAGAAATTCAAGAAATTAAAGATGCAACAGCTGCATTAATCAACGCTAAAACTAACGCTGAACAAGAAAAAGCAAAAATTGATGCCAAAGAACAACTTACTGCAAAAATTCAAGAGGCAGATAAACTTAAAGAAAAATTAATTGATAGTGATTCTACAATAACTACGCTAAAAACTAATTTGGAAAAAGAAATTGCTAATGCTAAAGAAGCACTTAAAAAAGATACTAAGGCAATTATTGAAGCAAAAAAACAATTAGCAAACAAAATTGATGAATCTAAATCAAGTCTTGACAAGTTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAAAAAGCAAGGAATAATATTAATAATTTCTTAACAGATAATGTTAAAAACAATCCAAATTATGCTGAACTTGTTAAAAAATTACAAAATTCTTTAAAAACACAAGAAAAAATTAACAACACTTCAAACAAAGCAGATATTATCAATGCAAATAATGAATTAACTAAGGCATTACAAGAAGCAAAAGTAGCAAAAGAACAAGCAGATAGAGCAAATGGCGAAGCTAATAATAAATTAACCGCCAGTTTATCTACTGCAAATGAATTATTAAAAAAATTAGTTGATTCAGATGCTGATATTCAAAATTATAAAAAACAACTTGAAAAAGAAATAGAAAAAGCAAATAATGCAATTACTTCAAAAAATACAAAATCATTGACTGATTCAAATGATTCATTAGCAAAGAAAACTAATGAAATTCAAGAAAAACTTGATAAATTCATAGAATCAAAAAATAAAGAATTTAAAAATTTCAATGAAACTAAAAAAGAAATTGATAATTTTATAAGTCAAATTGAAAAGGATCCTAAAACTAAAGGCAATTATTCGAATATAATTAATAAATTAAAGAATAAAAAGACTGAAAAAAACGCAATAAATAAAAATTCTGATAAAAAAGACATAGTAAATGCTAATACTGAACTTAAAAATTCTTTAAAAGAAGCAAAAGAACAAAAATCAGAAATTGATAAGTTTTATGCTGCTAAAAAAGATTTGGAAACATTAATATCAAAACCAGATGCTAAAAAAATAGGAATTAATGAAACCCAAAAGTTGTTAGACAAACACAAAACCATAAATGATGCCACAGATAAAGAAACAATCAAAAACGCAACAAAAGAATTGAATGATGCCAAAACTAATTTAGAGCAAAAAATTAAAGATAAGAAAGATAAATTATTAAAAGATTTCAATAAGGCAAAAGAAGATTTGGAAAATTATCAACAAGATGTTGATGTTTCTTCATTAGATGGTTTGGATCCAATTAAATTCTTTGATAGTATTCAAAAAGTTTTAGATATATTTAATAATGTAAGAACAATAAATGAAAATTCGACAATAGAAGAAATTGAAACATCAATATCGACATTAAAACACACAAAAGCTAATGCAATCAAGGAAGTGGAAAAACACCGTGAATTAACAAAATTGCATAAAACTATTGAAAAAGCTGAATCTTTTAAAAAAGAATTAGAATCGGAATTGAAAAATAATAATGATCATATACTTTCGACAATAAAGAATGATTTAAATTCAGCAATAGAAGATGCTAAAAATATTAAAATTGATGACGCAAAAATAATTAGCGATGAAAATAATAAATTAGAATCAAAAATTTCTTATTCAAAAGAAGCGTTTGAAAAATTTAAAGAATATAAAAAGTTAGAACAAACAAGAAAAGATATTGATGAATTTATAAAACAGATTGAAAATGATCATCAAACCAAAAACAATTATCTAAATATAGTTAAAAACTTAAAAAATAAAAAAATTGCTAAAGATTCAATAACTTTTTCTAATAATAAAAAAGAAATTCAAGATGCATATCAATCATTGCAAAATGAATTAAATAATGCAAAGATTACAAAAAAAGATATTACTGATTTTTACAACAGCAAAAAACAATTAGAAGATTTGATAAAAACTGATGATGCTAAAAAAGTAGGAACTACAGAAGCAGATACAATTTTAACCCATTATAAAAATATATCGGATGCTTCCAAAAATGAAGAAATTAAACAGGCAACCCAAAAAATAAATGGTATTAAAAAAATAATAGAAACTAAAATTTAA", "aa": "MKKRINILMSISIVCAAASTSAIAAMCSISNNSTKKYNEAKSRLLNLIKKLDNEGQKKANDFIARQDKKFNSTAFKNHSNTSKLIDEIEIFSKKILENLQKDEKQRLEELNKLNKLRLDLQNLINSNDGQNVDSSDAKKALNENQIDDSLPIDKIKKTKENLENAKKELLNKINTEKELQSKIFNDKKQELKRVLDLEDAKEVDSIKEQKVYIETNISETSSIENIKNKIIEVEKATSSLTSKILNTKQQELQEFENIKKDLQDFINTKLNDAKYQSIKQKALDKINSLNGINKNSTIKEIKAGQNALIKAKEEAGLEKEKLDGQNIKDTLKETINNAKEFKKLLIDNDQKIVDLKSNLDNEISKAEQSLSKDKESMESANDLLNTKLIEYKEILNKFNQEKEVKFNELEQTRKNIDNFLTDDVKNNPNYATLVKDLTNAKDDKKSVTKSSNKSDIIAANEALIQALADANKAKDQVDEANKSIKEQLNALIDKANTLLPQLNDNDSEIVKAKESLNAEITNANKAVNQNDNASMQSAKSSLDDKVTKIQNQLTEFNKDKDAKFKELEQARKNIDNFLTDDVKNNPNYATLVKNLTNAKDAKKSVTKSSNKSDIIAANEALKQALDKAKVAKEQIDDANKSIKEQLSDSIDNAKQLLNKLIDSDKDIQKAKTELNQEIQSANQALNLNNPTSMQSAKESLDAKVTEITKKLETFNKDKDAKFKELEKTRKDIDEFINSNKNNSTYSNIISKLTTTKNEKNSVSESSNKLEIEDANTLLKNSLKEANDKKQEIDKFVSAKKELEEFIENDEDTKSDELVSNLNDAKQALAKFSSIDDSHEITEIKKATDDLTTAKTKLKEEIKNKKQELFNAFESSKTELQNYITNDIKDSKYKIIQDKFNNVIDEYKNITSNDKISKIKEATNNLNAKFTEIGSNKAILDKFYELKSKLEDLLKTANLKDDAKSINIDLSSEDQAFADAKAINNNSTIENIEQVNQKLQTAIDKLAEDIKKIKEDKKAKFGEFKTEKEHLENIIKTKGLDKFINIDDEIKSIKKQIDEEIKKHKINENSKISDILAAKNSIDETQRKLLGSLDNYSTQRTESIDNYNKQKTELNSLTNSNNFKQLEESKKTKFNNKKQEYEVLNLETLSKNKIQEKSQEIKKLIDNINKEISDAKKQEYSKFDKAKKELEKYINDELNNQKYDSIKKEAQNKIDSFSTIKEESLTTKEIQEIKDATAALINAKTNAEQEKAKIDAKEQLTAKIQEADKLKEKLIDSDSTITTLKTNLEKEIANAKEALKKDTKAIIEAKKQLANKIDESKSSLDKFNKDKDAKFKELEKARNNINNFLTDNVKNNPNYAELVKKLQNSLKTQEKINNTSNKADIINANNELTKALQEAKVAKEQADRANGEANNKLTASLSTANELLKKLVDSDADIQNYKKQLEKEIEKANNAITSKNTKSLTDSNDSLAKKTNEIQEKLDKFIESKNKEFKNFNETKKEIDNFISQIEKDPKTKGNYSNIINKLKNKKTEKNAINKNSDKKDIVNANTELKNSLKEAKEQKSEIDKFYAAKKDLETLISKPDAKKIGINETQKLLDKHKTINDATDKETIKNATKELNDAKTNLEQKIKDKKDKLLKDFNKAKEDLENYQQDVDVSSLDGLDPIKFFDSIQKVLDIFNNVRTINENSTIEEIETSISTLKHTKANAIKEVEKHRELTKLHKTIEKAESFKKELESELKNNNDHILSTIKNDLNSAIEDAKNIKIDDAKIISDENNKLESKISYSKEAFEKFKEYKKLEQTRKDIDEFIKQIENDHQTKNNYLNIVKNLKNKKIAKDSITFSNNKKEIQDAYQSLQNELNNAKITKKDITDFYNSKKQLEDLIKTDDAKKVGTTEADTILTHYKNISDASKNEEIKQATQKINGIKKIIETKI", "aa_hexdigest": "b1c9941e1eca7b2b8747c01647497cd3", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00158C9899", "ncbi_nrp_id": "WP_175936686.1", "uniref100_id": "UniRef100_UPI00158C9899", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C9899", "RefSeq:WP_175936686.1", "UniRef:UniRef100_UPI00158C9899" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C9899", "uniref90_id": "UniRef90_A0A454CA13" }, "psc": { "uniref90_id": "UniRef90_A0A454CA13", "uniref50_id": "UniRef50_A0A454CA13" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA13" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 158, "aa_cov": 0.08169596690796277, "hmm_cov": 1.0, "evalue": 1.3383504179812553e-270, "score": 881.792724609375, "start": 494, "stop": 651 } ], "seq_stats": { "molecular_weight": 220465.33150000192, "isoelectric_point": 8.502815818786619 }, "id": "LMJBGEANGM_432", "locus": "GCA013368015_00408" }, { "type": "cds", "sequence": "contig_1", "start": 226406, "stop": 226915, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2", "UniRef:UniRef90_D1J7V2" ], "nt": "TTGCAAATTAGTAAAAATATAAAAATTGATCCGGTTGTAATTAATTCAGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACACCCTGAGTTGAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCTGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGACGCTCATAATTTCATCTTTTCAAAAATTTTAACAAATCAAACATTTACTGAAATGAAATTCTTAAAAATGAGATTTTCTAATTTGGAATTAGACACATCTGATAAAGAATCAAATAAACTAAAGGTAACATATGAAGTTTATTTAAATTATGAATATGCAACGGGAAGCTTCGAACTTAATAACGTTAAGGGCACTTCGCAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCTGAAAAGTGA", "aa": "MQISKNIKIDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEK", "aa_hexdigest": "399e16baf48697a98b72984906f2f9ac", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 226244, "stop": 227218, "observations": { "mutation": [ 226388, 226913, 227009, 227048, 227159 ], "stop": [ 226388, 226913, 227009, 227048, 227159 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V2", "query_cov": 1.0, "subject_cov": 0.49271137026239065, "identity": 0.988, "score": 330.0, "evalue": 7.43e-112, "gene_start": 1, "gene_end": 169, "reference_start": 55, "reference_end": 223, "reference_sequence": "MKNHSNTRRKHRSLAPLYYTLGSIAAAATIAGAIYFVVKNGRRVSKQYWSIEKFLQISKNIKLDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEKWNLNSKFANNLEKNLDDARKIIRIGIVKNDPKWTQSNEFREMIFKWFKRIFIENDAFPDTYSKDEYEIMPFNETSSGDDFVRWNPKKGLNNLTIKFFYVNKKNPDIKSPGRIKIFTILKV" }, "paralog": false, "identity": 0.9753086419753086, "score": 627.0, "evalue": 1.38e-231, "description": "Internal stop codon at 226388, 226913, 227009, 227048, 227159. Nonsense mutation around 226388, 226913, 227009, 227048, 227159.", "psc": { "uniref90_id": "UniRef90_D1J7V2", "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V2", "UniRef:UniRef50_D1J7V2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19863.4035, "isoelectric_point": 9.236852836608886 }, "id": "LMJBGEANGM_433", "locus": "GCA013368015_00409" }, { "type": "cds", "sequence": "contig_1", "start": 227277, "stop": 227606, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_044730145.1", "SO:0001217", "UniParc:UPI0005CD28E7", "UniRef:UniRef100_UPI0005CD28E7", "UniRef:UniRef50_D1J7V3", "UniRef:UniRef90_D1J7V3" ], "nt": "ATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTGAAAACCCTTTATTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCCAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTACAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAA", "aa": "MEIINKDLEAKLKTFQKQNYKLQKICQYFKIDLKKLNLNEFLDFNKNSENPLLDLKQIKQIIAENQGDSNILEVLNMIKELESINKEKTTNDETYKQDQILKKILSNLE", "aa_hexdigest": "a828eb2736d3fab7a0a2ef5aa6a9f5f6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005CD28E7", "ncbi_nrp_id": "WP_044730145.1", "uniref100_id": "UniRef100_UPI0005CD28E7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CD28E7", "RefSeq:WP_044730145.1", "UniRef:UniRef100_UPI0005CD28E7" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CD28E7", "uniref90_id": "UniRef90_D1J7V3" }, "psc": { "uniref90_id": "UniRef90_D1J7V3", "uniref50_id": "UniRef50_D1J7V3" }, "pscc": { "uniref50_id": "UniRef50_D1J7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12947.819699999985, "isoelectric_point": 6.357861900329589 }, "id": "LMJBGEANGM_434", "locus": "GCA013368015_00410" }, { "type": "cds", "sequence": "contig_1", "start": 227677, "stop": 227871, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAACAAACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGA", "aa": "MKTQKRINIAIDGPAGVGKTIMSTMLAKTLGYKFLSSGSFYRVVAYNALQNKLDLNDEESINNA", "aa_hexdigest": "3dea6507bae1c9cfbff26b5f509cc623", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 54, "aa_cov": 0.84375, "hmm_cov": 1.0, "evalue": 1.0088217980251335e-17, "score": 60.10975646972656, "start": 9, "stop": 62 } ], "seq_stats": { "molecular_weight": 7012.007700000001, "isoelectric_point": 9.455336189270021 }, "id": "LMJBGEANGM_435", "locus": "GCA013368015_00411" }, { "type": "cds", "sequence": "contig_1", "start": 227962, "stop": 228117, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGGCCGCATCAGCAATAGCAAAATTTCCTTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATATAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTACCGCAGGCAGAGGTTAAATTCTTTTTGTGA", "aa": "MAASAIAKFPLIRAKVNKFIQSFGLKYKGIIVDGRDATYRILPQAEVKFFL", "aa_hexdigest": "022ecf965105dc92e068de5a1e762d7b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 50, "aa_cov": 0.9803921568627451, "hmm_cov": 0.98, "evalue": 7.64564725973187e-12, "score": 40.899166107177734, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 5745.824500000001, "isoelectric_point": 10.21090564727783 }, "id": "LMJBGEANGM_436", "locus": "GCA013368015_00412" }, { "type": "cds", "sequence": "contig_1", "start": 228166, "stop": 228360, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "TTGGGTTTGAAGGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTATAATGACACAAACAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAA", "aa": "MGLKADYNEVLEDIKIRDYNDTNRKFDPLKISEGSIVIDTSNMSIQENFDVMYKEVRKRLDDEK", "aa_hexdigest": "d8380e0f2c4f50bd1230179322d30ea0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 56, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 1.2786669086298056e-12, "score": 43.43675994873047, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 7587.4448999999995, "isoelectric_point": 4.679520225524903 }, "id": "LMJBGEANGM_437", "locus": "GCA013368015_00413" }, { "type": "cds", "sequence": "contig_1", "start": 228350, "stop": 228493, "strand": "+", "frame": 2, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "nt": "ATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCTGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGA", "aa": "MRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAI", "aa_hexdigest": "62f2c355d3a368a5bd3cf56b2c965ad1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A7C7AIQ0", "query_cov": 0.9574468085106383, "subject_cov": 0.8035714285714286, "identity": 0.711, "score": 68.2, "evalue": 2.66e-14, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A7C7AIQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A7C7AIQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "product": "GTP-binding protein" }, "genes": [], "id": "LMJBGEANGM_438", "locus": "GCA013368015_00414" }, { "type": "cds", "sequence": "contig_1", "start": 228692, "stop": 229231, "strand": "+", "frame": 2, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_Q98RC1", "UniRef:UniRef90_D1J7V5" ], "nt": "TTGTTGGCAGTAAATAAACTTGAAGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCACTTGGATTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAACTTGTTGGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAATCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACCTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGTACAACGCGAGACTCTGTTTCGTCTCTAATAAAAATTAACGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATTGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGA", "aa": "MLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNK", "aa_hexdigest": "f5b7a3286fb944d6407954e0a267f0d7", "start_type": "TTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "both", "pseudogene": { "start": 228392, "stop": 229534, "observations": { "mutation": [ 228491, 229229, 229307 ], "stop": [ 228491, 229229, 229307 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V5", "query_cov": 1.0, "subject_cov": 0.40589569160997735, "identity": 0.9940000000000001, "score": 340.0, "evalue": 2.11e-114, "gene_start": 1, "gene_end": 179, "reference_start": 116, "reference_end": 294, "reference_sequence": "MMRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAIWTGKKFRVVDTGGITIEDEDFKKQIKLQAQIAIDEANVIVFLIDGKEPLTVEDYYVASLLRKSNKHVLLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNKWDLIEKDTNTMEKYKKDLLKKVKFVDWSPIVFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE" }, "paralog": false, "identity": 0.9921052631578947, "score": 720.0, "evalue": 2.17e-266, "description": "Internal stop codon at 228491, 229229, 229307. Nonsense mutation around 228491, 229229, 229307.", "psc": { "uniref90_id": "UniRef90_D1J7V5", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_Q98RC1", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V5", "EC:3.6.5.-", "UniRef:UniRef50_Q98RC1", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RC1" ], "product": "GTPase Der" } }, "id": "LMJBGEANGM_439", "locus": "GCA013368015_00415" }, { "type": "cds", "sequence": "contig_1", "start": 229319, "stop": 229672, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "GTGTTTATATCAGCCAAAGATAATTTGAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAATAAAAACAAAAAAGAAGAGAGAGAATAA", "aa": "MFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE", "aa_hexdigest": "51debaa0839e8831ce52cb33b0871601", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.6923076923076923, "hmm_cov": 1.0, "evalue": 2.729975002141588e-28, "score": 93.66259002685547, "start": 28, "stop": 108 } ], "seq_stats": { "molecular_weight": 13671.543899999982, "isoelectric_point": 9.982042884826662 }, "id": "LMJBGEANGM_440", "locus": "GCA013368015_00416" }, { "type": "cds", "sequence": "contig_1", "start": 229675, "stop": 229995, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02826.25", "PFAM:PF02737.24", "PFAM:PF02558.23", "PFAM:PF03446.21", "PFAM:PF02153.23", "PFAM:PF03721.20" ], "nt": "ATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCTGTGGCAACTATCTTAGCAAATAATGGTCATAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGA", "aa": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGF", "aa_hexdigest": "029ab543a64c457495d1fc070900fcf8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02826.25", "name": "2-Hacid_dh_C", "length": 104, "aa_cov": 0.9622641509433962, "hmm_cov": 0.875, "evalue": 1.8570094643432344e-07, "score": 26.19732666015625, "start": 4, "stop": 105 }, { "id": "PF02737.24", "name": "3HCDH_N", "length": 75, "aa_cov": 0.5849056603773585, "hmm_cov": 0.9866666666666667, "evalue": 3.241364063101085e-08, "score": 29.20623207092285, "start": 4, "stop": 65 }, { "id": "PF02558.23", "name": "ApbA", "length": 99, "aa_cov": 0.9150943396226415, "hmm_cov": 0.9797979797979798, "evalue": 2.070303581863995e-09, "score": 32.73456573486328, "start": 4, "stop": 100 }, { "id": "PF03446.21", "name": "NAD_binding_2", "length": 104, "aa_cov": 0.9433962264150944, "hmm_cov": 0.8846153846153846, "evalue": 7.67894162311159e-10, "score": 34.553951263427734, "start": 4, "stop": 103 }, { "id": "PF02153.23", "name": "PDH_N", "length": 88, "aa_cov": 0.8301886792452831, "hmm_cov": 0.8977272727272727, "evalue": 1.328339757528912e-07, "score": 26.760461807250977, "start": 16, "stop": 103 }, { "id": "PF03721.20", "name": "UDPG_MGDP_dh_N", "length": 86, "aa_cov": 0.7358490566037735, "hmm_cov": 1.0, "evalue": 8.845864512936772e-08, "score": 27.44905662536621, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 11723.46249999999, "isoelectric_point": 8.990712547302245 }, "id": "LMJBGEANGM_441", "locus": "GCA013368015_00417" }, { "type": "cds", "sequence": "contig_1", "start": 230026, "stop": 230658, "strand": "+", "frame": 1, "gene": "gpsA", "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0240", "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65", "UniRef:UniRef90_D1J7V6" ], "nt": "ATGACACAAGAAGCCCAAACCAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGACATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGTGGGGGTAAGCTGAAAACGATTTTTGGATTGTCGTGCCTGGGTGATTTAATGCTAACAGCATTGTCGGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCACTTTATCAAATAATATACAAGAATGATAACCCTAATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAA", "aa": "MTQEAQTNSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNDNPNEIIINLMKRKLKSE", "aa_hexdigest": "b1dcdf92481faa2c6a6d39a2fe89c0fe", "start_type": "ATG", "rbs_motif": null, "genes": [ "gpsA" ], "truncated": "5-prime", "pseudogene": { "start": 229726, "stop": 230658, "observations": { "mutation": [ 229993 ], "stop": [ 229993 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V6", "query_cov": 1.0, "subject_cov": 0.6422018348623854, "identity": 0.99, "score": 396.0, "evalue": 2.26e-137, "gene_start": 1, "gene_end": 210, "reference_start": 118, "reference_end": 327, "reference_sequence": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGFWKDSNKSCHEIMTQEAQDNSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPNEIIINLMKRKLKSE" }, "paralog": false, "identity": 0.9903225806451613, "score": 598.0, "evalue": 1.44e-219, "description": "Internal stop codon at 229993. Nonsense mutation around 229993.", "psc": { "uniref90_id": "UniRef90_D1J7V6", "gene": "GpsA", "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z5IR65", "cog_id": "COG0240", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V6", "UniRef:UniRef50_A0A2Z5IR65", "COG:COG0240", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IR65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65" ], "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]" } }, "id": "LMJBGEANGM_442", "locus": "GCA013368015_00418" }, { "type": "cds", "sequence": "contig_1", "start": 230673, "stop": 230954, "strand": "+", "frame": 3, "gene": "hupA", "product": "HU family DNA-binding protein", "db_xrefs": [ "COG:B", "COG:COG0776", "RefSeq:WP_020002655.1", "SO:0001217", "UniParc:UPI0002F8284C", "UniRef:UniRef100_UPI0002F8284C", "UniRef:UniRef50_N9UB80", "UniRef:UniRef90_A0A454C9F8" ], "nt": "ATGAACAAAAAAGAACTACTAAGTAAAACTAGCAATGAAACAGGATTTGCACATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGACGAAATAGAGGCAGGAAGAGAAGTTACAATTTCGGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCACAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTCAAATTCTCAACAGCTTTTAAAAAACGTATTAACAGCAAAAATTAA", "aa": "MNKKELLSKTSNETGFAHVLIESAFDEIIRIIADEIEAGREVTISGFGNFSSKFIKGKEKVHNITKEIINVPDRLDPRFKFSTAFKKRINSKN", "aa_hexdigest": "c995b4f9d9cab48df52b1eddb90ad6cd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002F8284C", "ncbi_nrp_id": "WP_020002655.1", "uniref100_id": "UniRef100_UPI0002F8284C", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F8284C", "RefSeq:WP_020002655.1", "UniRef:UniRef100_UPI0002F8284C" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F8284C", "uniref90_id": "UniRef90_A0A454C9F8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9F8", "gene": "HupA", "product": "HU family DNA-binding protein", "uniref50_id": "UniRef50_N9UB80", "cog_id": "COG0776", "cog_category": "B" }, "pscc": { "uniref50_id": "UniRef50_N9UB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UB80" ], "product": "DNA-binding histone-like protein HU" }, "genes": [ "hupA" ], "id": "LMJBGEANGM_443", "locus": "GCA013368015_00419" }, { "type": "cds", "sequence": "contig_1", "start": 231280, "stop": 231483, "strand": "-", "frame": 3, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_A0A077L6J4", "UniRef:UniRef90_D1J7V7" ], "nt": "ATGAAAAATAAAAATAATAGAGGTATGCTTTTAGAATCTATAATAAATAACACTAACGAGTATTATTTTAAAAATGGAATTGCTTTAATTCATAAAAAAAATTTGGATATTAAATTTAAAAATGTTAAATTAGAAAAAAATAAATTGCTTTTAAACGATGCAAGTATCTATTCAAAAAGCACAGTAGATTATTATGGAGTTTGA", "aa": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNDASIYSKSTVDYYGV", "aa_hexdigest": "6ba5c8dd1bc0835597e28ef2ab389482", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 230980, "stop": 231483, "observations": { "mutation": [ 231156, 231282 ], "stop": [ 231156, 231282 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V7", "query_cov": 1.0, "subject_cov": 0.40119760479041916, "identity": 1.0, "score": 130.0, "evalue": 2.08e-37, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNDASIYSKSTVDYYGVWKGRFLAFEVKSTEQKNFEKKNIKKHQIEYLNKVLLHQGIAFWIIYFKFQNEFLLIEHANFKKCLFEEKKSYICFEALKSLGKEIKLIFPGILDFLNILK" }, "paralog": false, "identity": 0.9640718562874252, "score": 320.0, "evalue": 1.06e-114, "description": "Internal stop codon at 231156, 231282. Nonsense mutation around 231156, 231282.", "psc": { "uniref90_id": "UniRef90_D1J7V7", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A0A077L6J4", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V7", "EC:3.1.21.10", "UniRef:UniRef50_A0A077L6J4", "COG:COG3331", "COG:R", "KEGG:K03700" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6J4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6J4" ], "product": "Holliday junction resolvase RecU" } }, "id": "LMJBGEANGM_444", "locus": "GCA013368015_00420" }, { "type": "cds", "sequence": "contig_1", "start": 231521, "stop": 232099, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGTAATAAATGATTTATATGATGAGAAAGCAGATTATTATGTCGTATATAACGACACATATGAAGCATCTAGAATAAAAGAAATGCAAGAAATTTTGGAATATTACAAAGATGATGGATTTATTAGCCAAAATGAATTTGACATTTTAAAAGAAAAATCTCAATTTATATTAAGCAAGATTATTGATATAGAAAATTTATTTTTTAGACCAGCAATGGATTGCAATATAAAAAGCTCTATTGTAAATTTAGGGCAAATAAAAAATGTCTATTCAATAAAAAAGATTGAGCTTTTTGTAAATGAAAATAAATTAAATTTAAAACATAAAATTTTTCCATATCACGAACTTGATGTAAAAAATGGTGGAATGGCCTTACAAATAGCGACACTAAGGGCCTTAAACAAAATAAGAGATAATGAATGGACAGTCAAAGAAGCTCAATTGAAAAGATATTGCCATAATGATGTTTTGGCAATGATTATGGCATTTGACTTAGTTGAATATTTATTAAAATCAAAAAATATTTATTTTAAAAACTTCGAAACATATAAAGACTTAAAAGCGAAAAAATAG", "aa": "MKVINDLYDEKADYYVVYNDTYEASRIKEMQEILEYYKDDGFISQNEFDILKEKSQFILSKIIDIENLFFRPAMDCNIKSSIVNLGQIKNVYSIKKIELFVNENKLNLKHKIFPYHELDVKNGGMALQIATLRALNKIRDNEWTVKEAQLKRYCHNDVLAMIMAFDLVEYLLKSKNIYFKNFETYKDLKAKK", "aa_hexdigest": "377cc5db73cef59956a7991cab5d9e2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22855.228300000017, "isoelectric_point": 7.6536771774292 }, "id": "LMJBGEANGM_445", "locus": "GCA013368015_00421" }, { "type": "cds", "sequence": "contig_1", "start": 232205, "stop": 232522, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATGACTTAAAAGATAATTTAAATTTAGGAATCAAATTCTACCAATTATTAGTTAAAAAGGTTATTCCTAAATACATTATTTGTCAAAAGGAATTATTAAAATTAAAAGTTAAATCATTAACTGATAATTCAATTGAAACCAAAGAAATTTTAGATTTTTATGAAAACAATTTAATTTCAATTTCACCCGATATTTTAAACAAGGATAAATGCCCTGAATATTTATTATTAAAAGAATTTAATAAAAAAATAGTTTGGTTTGATTTCGAAGGCGTTACCTTGCCCGTTCCAATGATTAACCACTCTCTAGCATGA", "aa": "MDDLKDNLNLGIKFYQLLVKKVIPKYIICQKELLKLKVKSLTDNSIETKEILDFYENNLISISPDILNKDKCPEYLLLKEFNKKIVWFDFEGVTLPVPMINHSLA", "aa_hexdigest": "3a8be50411da49241edc189840606b83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12287.418499999983, "isoelectric_point": 6.566972541809082 }, "id": "LMJBGEANGM_446", "locus": "GCA013368015_00422" }, { "type": "cds", "sequence": "contig_1", "start": 232895, "stop": 233527, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATTTTAGTGAAGTTTATGAAAGCGACAATCAAGAAGACAACGGTTATGAAATGTTGGAATTTGCAAATGATGATATTGAAAACATTCTTGACAAAAATTTTAATCAATACTTTGAAAATAACTCTACTAAAAACAAAGACTTTTTAAAGCTAAACACTATTGAAAAAATAAAAAAAATTCAACATGATATGCAAAATGATTCAACACTGTTTGAAAAAATTAATTCAGATGGTAAACTAGAATTATTTAACAAAATATTGGATAATTTTTTTCAGTTTCAAATGGTTGATAGTGGAATAGCCCTTGTTGAGGAACATATTATAAATTTTTTAACAAAAAATTCTTCAAATAAAGTAAATATAATTTCTAAAAAATCTTCAACCGATGAAAAAATTTTACAAACAAAAGAATTTTTAATGCAAAATTCGCTAATAATAAATCCTGTTTTTGAATATAAAAAATGCACATCAAAGCCTTTTTACTACGATAGTAAAACTAAAGAAATGGGAAATATTGTATATTCATCAAAAACTAGCCTAAAAGATTTATTAAGAGCATATTATGATTATTCCGTTCTTTCATCCTTAGGAATTGAAATCAATAAAATTTTCATTGTGAAACCTTGA", "aa": "MNDFSEVYESDNQEDNGYEMLEFANDDIENILDKNFNQYFENNSTKNKDFLKLNTIEKIKKIQHDMQNDSTLFEKINSDGKLELFNKILDNFFQFQMVDSGIALVEEHIINFLTKNSSNKVNIISKKSSTDEKILQTKEFLMQNSLIINPVFEYKKCTSKPFYYDSKTKEMGNIVYSSKTSLKDLLRAYYDYSVLSSLGIEINKIFIVKP", "aa_hexdigest": "34423775299274b7beea3028b9692a3c", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24602.528500000026, "isoelectric_point": 5.01788272857666 }, "id": "LMJBGEANGM_447", "locus": "GCA013368015_00423" }, { "type": "cds", "sequence": "contig_1", "start": 233985, "stop": 234455, "strand": "-", "frame": 1, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0", "UniRef:UniRef90_D1J7V9" ], "nt": "ATGGAAAAAAAAATAATAATTCCTAAAAGAACATTTTTTAACAATAAAAATTACACTTTGGATTATAAAAATAAGACTTTAATAGTAGATCCAAAAATCTTAAAATCTATGCAAGAAAAAAAACCTGGTACTTTTGGAGGATTTAAAAAAATGACCGGTTCGGCGTTGGGAGCTATAATGGGATTGAGCTCATTTGACAGCCAATTTGAAGCATTTTGTAATATATGCGGTTTTAGAAAACCAATATTGAACAGGAAATATGTTGATGCAGGTATTGCATTAGAACCAAAAATAGTAAAAATTATTGAAAAAAAATTAAATTCCAAATTGCTTAGATATGAAGCCAATCAATATAATTATGATTATTTTAAAGATAACGAACTATTTGGAGGGTTGCCTGATGGCTATAGTCCCGAAAGACATTTAATTATTGAAATTAAAACTGTCGGGGAAAAAAATTATGAAAATTGA", "aa": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYEN", "aa_hexdigest": "62555fefa421922b2ff76884a03c3221", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 233682, "stop": 234455, "observations": { "mutation": [ 233732, 233771, 233828, 233987 ], "stop": [ 233732, 233771, 233828, 233987 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V9", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 1.0, "score": 318.0, "evalue": 2.92e-108, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYENWIKYGVNPGYIKQAQLYTYLIGATSFSIVACFLKEEDYLNPNNVDITKRIIKNWTYILNNEQVQDDINYCMQWYKKYTQIGISPKWNEQIDQDLLKFLECNNQEEWKNLYLYWVDQGKAVPEYEQA" }, "paralog": false, "identity": 0.980544747081712, "score": 509.0, "evalue": 3.54e-186, "description": "Internal stop codon at 233732, 233771, 233828, 233987. Nonsense mutation around 233732, 233771, 233828, 233987.", "psc": { "uniref90_id": "UniRef90_D1J7V9", "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V9", "UniRef:UniRef50_A0A077LBU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0" ], "product": "YqaJ viral recombinase domain-containing protein" } }, "id": "LMJBGEANGM_448", "locus": "GCA013368015_00424" }, { "type": "cds", "sequence": "contig_1", "start": 234690, "stop": 235487, "strand": "+", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_036438978.1", "SO:0001217", "UniParc:UPI000514027E", "UniRef:UniRef100_A0A454CA24", "UniRef:UniRef50_D1J7W0", "UniRef:UniRef90_D1J7W0" ], "nt": "ATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTAGTAGCAGCAGGATGCAATGATCCAAAGAATAAGAAAAACCCAAACAATAATGATCAAAGTCAAGATAGCCAAATTGAGTTTCAAGAAGGCTTGGGGTTAAAAATTGCAAAAATCGCCAAAGATCAAGAAAATGTTAAAGTTGATGAATTTGTAAAAGAAGCAAAGGGTGCAAAAACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGAAGCAAAATATGAAGTTGCTGCTGGTACACACGGTCATGCCGATGAAGGACTAGTACACTTAGATATTATTCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAATGGAAACTGTTGAAGACACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGGCTTTAATTCTTTACTTGAAAAATTAAAAGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACGAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAA", "aa": "MKKYTKILLTLGAISTSIVAVPLVAAGCNDPKNKKNPNNNDQSQDSQIEFQEGLGLKIAKIAKDQENVKVDEFVKEAKGAKTLDQIKALFTKYKIAYDLSEIPDEAKYEVAAGTHGHADEGLVHLDIIQTVNEKQNNNRFEIKGFKMETVEDTVQLGGYKLSTKILDNKKDFSLQEIKKQIIDAQEKGFNSLLEKLKEFVNIEVDENNKTKQFEFNKEKIELISQSGQIVFKGISIIDISKKVNEVGYKTESKTDFIIQGLNKVE", "aa_hexdigest": "cced00f4f0408bd24d8aaf0948a642c1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000514027E", "ncbi_nrp_id": "WP_036438978.1", "uniref100_id": "UniRef100_A0A454CA24", "db_xrefs": [ "SO:0001217", "UniParc:UPI000514027E", "RefSeq:WP_036438978.1", "UniRef:UniRef100_A0A454CA24" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA24", "uniref90_id": "UniRef90_D1J7W0" }, "psc": { "uniref90_id": "UniRef90_D1J7W0", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7W0" }, "pscc": { "uniref50_id": "UniRef50_D1J7W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W0" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_449", "locus": "GCA013368015_00425" }, { "type": "cds", "sequence": "contig_1", "start": 235722, "stop": 235919, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGCTAAAGCACTAACAATTATATTTACGACAAAATCGGGTGCTTTATTAAATTTATATGCACTTCAAATTCCTAGTAAATTTCCAATAATCAAAGAAAAAATAAAAATAATTACAGTAAATAAGATTGTTCATTTAAATTGCATAAAATACAATGTAATTATTGACAAATTAGTTGCTGCAAGCTCTTTTGAATAA", "aa": "MAKALTIIFTTKSGALLNLYALQIPSKFPIIKEKIKIITVNKIVHLNCIKYNVIIDKLVAASSFE", "aa_hexdigest": "4fd5767819528523a705778b33b34093", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7245.7593000000015, "isoelectric_point": 9.781288337707515 }, "id": "LMJBGEANGM_450", "locus": "GCA013368015_00426" }, { "type": "cds", "sequence": "contig_1", "start": 236119, "stop": 236466, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTATTTCACTAGGGAATTCAAAAACTAAATTATTTAAACAAAACATTATTAAAAATTTAATTATTGCTGAATTACAAGTCATTTTGCCTTTTTATATATTGCTTTTTAGTACTTCATTAATAATTGAGAGAATTTTTTCAATACCAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTGAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGA", "aa": "MFGISLGNSKTKLFKQNIIKNLIIAELQVILPFYILLFSTSLIIERIFSIPGQSIFISYVFTKAELNCIMFYFSFNFFALLIARFINEIILNYLNPAQAIEKRIRWIKRKRLDYE", "aa_hexdigest": "7e199641eb37af17c4d5ac9fde9b865b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13624.162099999976, "isoelectric_point": 9.809589958190916 }, "id": "LMJBGEANGM_451", "locus": "GCA013368015_00427" }, { "type": "cds", "sequence": "contig_1", "start": 236459, "stop": 237127, "strand": "+", "frame": 2, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7W2", "UniRef:UniRef90_D1J7W2" ], "nt": "ATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCTATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTCTTTAATAAAAAGAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTGCTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCCTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGTATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGCGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATTAATATTTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAATCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTCTAGCAGCATTTTATGTTTTTAATCGTTACAAATGA", "aa": "MNELDNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTISIILLAIFINIFIGFSLAAFYVFNRYK", "aa_hexdigest": "fa27c08ce8bd855306f6e8f24ed1bfe3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 236459, "stop": 237430, "observations": { "mutation": [ 237125, 237185, 237221, 237257 ], "stop": [ 237125, 237185, 237221, 237257 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W2", "query_cov": 1.0, "subject_cov": 0.556390977443609, "identity": 0.991, "score": 413.0, "evalue": 8.9e-143, "gene_start": 1, "gene_end": 222, "reference_start": 1, "reference_end": 222, "reference_sequence": "MNELNNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYKWYGIVIDKIAVTLNAIPEIIWIFLLCIFLGTNWYGILIAFILISWISFYELSKNEILNLIQKEYIKAAKIIGLNNFQITYRHLFKNVLPSLIILIADRLAINILIVASLAFLDFLTAESNLNIGTIFKEALSSIKVNPQYLIIVAVYITIFCILLKLFSIGLSMTYDPKISKIINRI" }, "paralog": false, "identity": 0.978328173374613, "score": 609.0, "evalue": 1.26e-222, "description": "Internal stop codon at 237125, 237185, 237221, 237257. Nonsense mutation around 237125, 237185, 237221, 237257.", "psc": { "uniref90_id": "UniRef90_D1J7W2", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7W2", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W2", "UniRef:UniRef50_D1J7W2", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W2" ], "product": "Oligopeptide transport system permease protein" } }, "id": "LMJBGEANGM_452", "locus": "GCA013368015_00428" }, { "type": "cds", "sequence": "contig_1", "start": 237118, "stop": 237246, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAACTAAAATACCATATCAATTTGTTCCCAAAAATATACACAACAAAAATATTCAAATTATTTCGGGTATTGCATTAAGTGTAACAGCAATTTTATCGATAACAATTCCATATCATTTGTAA", "aa": "MKATKIPYQFVPKNIHNKNIQIISGIALSVTAILSITIPYHL", "aa_hexdigest": "48b6091c9e9aebdc135720157be74f6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4690.592899999999, "isoelectric_point": 10.000351905822754 }, "id": "LMJBGEANGM_453", "locus": "GCA013368015_00429" }, { "type": "cds", "sequence": "contig_1", "start": 237745, "stop": 238275, "strand": "+", "frame": 1, "gene": "yqdH", "product": "Iron-containing alcohol dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1979", "SO:0001217", "UniRef:UniRef50_H3NQ88", "UniRef:UniRef90_D1J7W3" ], "nt": "ATGTTGAATTTTATTTATAAAAACGATTGCAAAATCATATTTGGAAAAGATTCAATTCAAAATTTAGAAGAAGAATTAAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAGTAATTAAGGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGTAATGTTGTTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATCGGTGGCGGTTCGGTAATCGACACCGCCAAAGCAATAGCAGTTGGGGCAAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGCGCATTACCTATTGGAGTTATTTCGACAATTGCATCTAGCGGTTCCGAATCGTCAAATTGCTCGATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTTGCAATAATTGATCCTTGA", "aa": "MLNFIYKNDCKIIFGKDSIQNLEEELKSLKVKKILIVYSGKYIFELKIYDEVIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDP", "aa_hexdigest": "6ac7fc3e628dd8569951cc351f813322", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [ "yqdH" ], "truncated": "3-prime", "pseudogene": { "start": 237745, "stop": 238578, "observations": { "mutation": [ 238273, 238531 ], "stop": [ 238273, 238531 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W3", "query_cov": 1.0056818181818181, "subject_cov": 0.45618556701030927, "identity": 0.983, "score": 341.0, "evalue": 1.71e-115, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 177, "reference_sequence": "MLNFIYKNDCKIIFGKDSIQNLEGELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQDEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.9784172661870504, "score": 535.0, "evalue": 3.27e-194, "description": "Internal stop codon at 238273, 238531. Nonsense mutation around 238273, 238531.", "psc": { "uniref90_id": "UniRef90_D1J7W3", "gene": "YqdH", "product": "Iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H3NQ88", "cog_id": "COG1979", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W3", "UniRef:UniRef50_H3NQ88", "COG:COG1979", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_H3NQ88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NQ88" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "LMJBGEANGM_454", "locus": "GCA013368015_00430" }, { "type": "cds", "sequence": "contig_1", "start": 238294, "stop": 238533, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATTTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAATGCTAAATTATTAATGGAAGATCCAAATGATATGAATGCAAGGTCAGAAGTATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGA", "aa": "MYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEVFLISIFAHNNMLDSGRVAD", "aa_hexdigest": "23b965e3c3e0931a1d406117acaf40a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8983.178399999993, "isoelectric_point": 4.593579673767089 }, "id": "LMJBGEANGM_455", "locus": "GCA013368015_00431" }, { "type": "cds", "sequence": "contig_1", "start": 238606, "stop": 238908, "strand": "+", "frame": 1, "gene": null, "product": "iron-containing alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3", "UniRef:UniRef90_UPI00215D79B7" ], "nt": "ATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTAGACAAGGGTTTGAGCCAAGAAGAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGATAGTCAAACCATTGGTCATTATAAACCCCTTTTATCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAA", "aa": "MVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK", "aa_hexdigest": "f29260e14884c0d1926e1f5fbbbbab27", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 238306, "stop": 238908, "observations": { "mutation": [ 238531 ], "stop": [ 238531 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D79B7", "query_cov": 1.0, "subject_cov": 0.425531914893617, "identity": 0.99, "score": 187.0, "evalue": 4.32e-58, "gene_start": 1, "gene_end": 100, "reference_start": 136, "reference_end": 235, "reference_sequence": "MSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKSLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEEKANLLSKKLDEFYRTIKMRTRLSELGVGCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.98, "score": 384.0, "evalue": 3.15e-138, "description": "Internal stop codon at 238531. Nonsense mutation around 238531.", "psc": { "uniref90_id": "UniRef90_UPI00215D79B7", "product": "iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D79B7", "UniRef:UniRef50_H1B6A3" ] }, "pscc": { "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "LMJBGEANGM_456", "locus": "GCA013368015_00432" }, { "type": "cds", "sequence": "contig_1", "start": 238960, "stop": 240144, "strand": "-", "frame": 3, "gene": "tktA", "product": "Transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J7W4", "UniRef:UniRef90_D1J7W4" ], "nt": "ATGAATTTCAAATACATTGAAGTATCAAATGGTCAATGTCGTTCAATAATAAAAGCAATAAAAAAAGCACAAAAAATTAATCAACCTGTGTTTATTAAAATTTTAAGTCCTCAAAGCGAACAATTATTTAAAAAGAACTTTGATAATTCAATAACTCATGAAAAATTAGAAGAATTCAAAGACAAAACCTCTTTTCAAAAAACAAATTTGAGTGATTTGTATTCTGAGGTTGTTGAGTTTTATGAAAATAGATTGCAAAAAATAGAAAACAAAATAAAAACATTTAGTAATTCAAATAATTTGCAAATCTTTTTAAAAAACAATATTCGTGAAAATATTAACGATGTTGAAGAATTTAAAGAAAAAACATACATTGAAAATGCTTTAAAAATAATAAATAATTTATGTGATAAATACAAAAATTTACTAGTATTATCAAATAATATTTCTTATTTTCAAAATATTAAATCGTTAAATGGGATTTTTGCAAGTAACAATCTTGGAAGAGGAATTTTCTTAGGTAATAGAGAATTTACAATGATTAATATGGCAATGGGATTAGGTTTGCATTCAAGCATAGAAACAATTGTCATTGTAGATATTAATTATTTAGGTTATTTATTGCCAACATTGCAAGTCTTAAAATATTCAAATGCAAAAATTCTTATACTATTAATCAATAATAGAAATAATTTTAGCCTAAGCAATGAGCTTAAAATATTATATTCATATGACCATATAAATTTATTGAGGCCTTGCGATTTCAATGAATTAAAAGGAGCATTTGAATTTCATTTTAATCACACAAAAAAGTCATCAATAATTTATTTAGAAAATAAAGAAATTAAAGAAATAAAGGATACTTCAAAAGTTGAATTTGAAGATGGCCCATATTACATTAGAAAAATTAGTCAAACAACAAATATTATAGCAGCAGGATCTGATCTTAGAATAGCAGAGATTATTGCTAGAAGGTTAAATAGTTCTATTATTTCAATATCAAATATTGAAAGCATTGAAAGCCTGAACTACGATAGAAATAATTCAATACTAATTGAATCTTATGAGGCATTATCTCATGAAAAAATTGCTAAATATAACATTGCATTAAATAAATTTTGCAACCTTGAAAAAAATGATGAGTATATTGTAGAAAAAGTTTTAACTTCAATAGTAAAAAAATAA", "aa": "MNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNNISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAEIIARRLNSSIISISNIESIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEYIVEKVLTSIVKK", "aa_hexdigest": "006e2f9e954e3143975981adfd3f8ed1", "start_type": "ATG", "rbs_motif": null, "genes": [ "tktA" ], "truncated": "3-prime", "pseudogene": { "start": 238960, "stop": 240444, "observations": { "mutation": [ 240315, 240429, 240441 ], "stop": [ 240315, 240429, 240441 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W4", "query_cov": 1.00253807106599, "subject_cov": 0.6605351170568562, "identity": 0.99, "score": 731.0, "evalue": 2.42e-262, "gene_start": 1, "gene_end": 394, "reference_start": 204, "reference_end": 598, "reference_sequence": "MKNKKIDQIAIDNIKINSLNMAFSWKYSDFSSIMSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLINNRNQNISYSNWTLDWIKSGEIFLGISGGFALGNTIAQNYLNRLDKNINHFTYWYVTERELQEGSTQEALAYAGTHNLRQLIVIYDANEIEYTVDSKSINPENIAKKVESMNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNNISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAELIARRLNSSIISISNIGNIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK" }, "paralog": false, "identity": 0.9858585858585859, "score": 946.0, "evalue": 0.0, "description": "Internal stop codon at 240315, 240429, 240441. Nonsense mutation around 240315, 240429, 240441.", "psc": { "uniref90_id": "UniRef90_D1J7W4", "gene": "TktA", "product": "Transketolase", "uniref50_id": "UniRef50_D1J7W4", "cog_id": "COG0021", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W4", "UniRef:UniRef50_D1J7W4", "COG:COG0021", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W4" ], "product": "Transketolase" } }, "id": "LMJBGEANGM_457", "locus": "GCA013368015_00433" }, { "type": "cds", "sequence": "contig_1", "start": 240439, "stop": 240654, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGGGCAAAAATATTTCATGCTCTTTTTGGCTATTTTTTCAATATAAATAGAAAAAATAATTCTGATGTCAATAGGGATAGATTTGTTTTTTTTAATCCCAACATATTGCCACTTTTTTATGAAATTTTGAGATTATATAGTTTAATTAGTGAAAATGAATACCTTAACTTAATAAGCAATAGAAATCAAAACATTTCATATTCGAATTGA", "aa": "MSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLISNRNQNISYSN", "aa_hexdigest": "bf272d45ac44cd0e2f7558d7c310d171", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8501.515799999992, "isoelectric_point": 9.220993614196775 }, "id": "LMJBGEANGM_458", "locus": "GCA013368015_00434" }, { "type": "cds", "sequence": "contig_1", "start": 240743, "stop": 241027, "strand": "-", "frame": 2, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5", "UniRef:UniRef90_D1J7W5" ], "nt": "ATGAACTTTCGTTATTTTGTTGAACAAGATGCAATATATGAAGAATTAAACAATATTTTTAGTTCAAATAAAAATATAAAATTAATTGGAAAAATAGAAATTAAACCAAATCGCACTAAAACAAATGTTTTTATTAATTTAAGTTACAAATTAATAAACAAAACTAGTTTTGCATATGAAACAAAAAAACTAATATTTAAGATTGAACATAAAGTTTTTTCATTAATAAATGCCAAACCAAACAATATTAATTTAATATTCCAAGGTTATGAAGATGAAAAATAA", "aa": "MNFRYFVEQDAIYEELNNIFSSNKNIKLIGKIEIKPNRTKTNVFINLSYKLINKTSFAYETKKLIFKIEHKVFSLINAKPNNINLIFQGYEDEK", "aa_hexdigest": "6260e9e8055bd6afa1fc15267aea064e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7W5", "query_cov": 1.0, "subject_cov": 0.912621359223301, "identity": 1.0, "score": 187.0, "evalue": 3.63e-60, "valid": true, "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_D1J7W5" }, "pscc": { "uniref50_id": "UniRef50_D1J7W5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5" ] }, "genes": [], "id": "LMJBGEANGM_459", "locus": "GCA013368015_00435" }, { "type": "cds", "sequence": "contig_1", "start": 241316, "stop": 241615, "strand": "-", "frame": 2, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_D1J7W6" ], "nt": "ATGATAAATGTTAACGATTTAAAACCAGGAGTTACATTTAAATTAGAAGGCAATATTTATGTAGTGCTTGAAGCAGCACATTCAAAACAAGGCCGCGGCCAAGCAAATGTTAAAGCAAAAGTTAAAGATCTAAGAACAGGTTCAACAACAATTAAGTCATTTACTGGTGGCGAAAAAGTTGAACCAGCAATGATTGAAAAATCAAATATGGATTATCTATACAATGCAGGAGACGTTGTAGTTTTAATGGATCAAGAAACTTTTGAACAAATTGAAATACCTTCGGAACATCTTAATTGA", "aa": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLN", "aa_hexdigest": "4dfa5bee95f1db1ddb640fba54f22fd7", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 241058, "stop": 241615, "observations": { "mutation": [ 241318 ], "stop": [ 241318 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W6", "query_cov": 1.0, "subject_cov": 0.5351351351351351, "identity": 1.0, "score": 196.0, "evalue": 1.38e-62, "gene_start": 1, "gene_end": 99, "reference_start": 1, "reference_end": 99, "reference_sequence": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLNWELNFLKEGQKVIVRKFQNEVLDIELPANVNLKVVEAPDAVKGNTAQAAQKKVTLETGYVVETPLFIKEGEIVTISTETGKYTGRA" }, "paralog": false, "identity": 0.9945945945945946, "score": 358.0, "evalue": 1.15e-128, "description": "Internal stop codon at 241318. Nonsense mutation around 241318.", "psc": { "uniref90_id": "UniRef90_D1J7W6", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W6", "UniRef:UniRef50_Q98Q55", "COG:COG0231", "COG:J", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "LMJBGEANGM_460", "locus": "GCA013368015_00436" }, { "type": "cds", "sequence": "contig_1", "start": 241687, "stop": 241947, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACCATTCGGAAAAGCTAATTCAGAAATTAACGCTAATGTTGAACTATCAATTCCACCAGATATTCCTAATGTTATACCTTTAGAATTTGAGGAGTTTATTTTTTGCTTTAATCAAGAAACTATTTTATTGATTAATATAGAATATATCTTCAATTCTTTTTTTGTCTTAGGAACTTTTCTTAATAAATCTTCCATTTTAACCTGCCTTATTTCAATTATCATTAAATTTAACAATAATAAATGTAAAATTCCATAA", "aa": "MQPFGKANSEINANVELSIPPDIPNVIPLEFEEFIFCFNQETILLINIEYIFNSFFVLGTFLNKSSILTCLISIIIKFNNNKCKIP", "aa_hexdigest": "b0742364295ca266fb13889d46967ff7", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9829.427999999985, "isoelectric_point": 4.644905281066895 }, "id": "LMJBGEANGM_461", "locus": "GCA013368015_00437" }, { "type": "cds", "sequence": "contig_1", "start": 242003, "stop": 242512, "strand": "+", "frame": 2, "gene": null, "product": "NAD(+) synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD8376" ], "nt": "TTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAATTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAGACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATATGGTCTTGCATTCCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAGGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGGTTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAGGGTCCAAAATTAAAATAA", "aa": "MQLKLKTKIEIINLSSCFKKIVKIANIIDQGSIANTKSRLFMTTLYGLAFQNKNLVLGTDNFDEYYLGYFTKYGDGGCDLLPFANIKKSDVYAMASLLGVPQEIIDKKPSATLYKNQYDEEELGFKYSDFEKYLLDPTSVSEELIKKIKNIHKQTEHKRKAIPKGPKLK", "aa_hexdigest": "6c82a6eb091e5d7e5e3d5a489affc069", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BD8376", "query_cov": 0.8757396449704142, "subject_cov": 1.0, "identity": 0.655, "score": 193.0, "evalue": 1.12e-60, "valid": false, "product": "NAD(+) synthase", "uniref50_id": "UniRef50_UPI0021BD8376" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD8376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD8376" ], "product": "NAD(+) synthase" }, "genes": [], "id": "LMJBGEANGM_462", "locus": "GCA013368015_00438" }, { "type": "cds", "sequence": "contig_1", "start": 243073, "stop": 243702, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53", "UniRef:UniRef90_UPI001E586E53" ], "nt": "TTGTACCAAATAGTTGTTGAAAAAAAGGCAAATGATATCCTAGCAAAAAACTATATTTGTGAAGATGTAACTTTTAAAATATCTGCTTCAAAATCTTTAAAATCAAATACTTTAGTATCTAATACGATGCTAAAAAAATATCCAATATTTATCAAAATAAATAGAGATGAAAAAAGCAATAAATTATCAATCACTCTCTCAATTCATAGATTGATTTATGAAGTTGCTACAAAAAAAGAAATTTCAATAAATAAAAAATCTCTAAATTTGTCGATTTATCATGAAACAAATGATGAATATATTCACTTTAAAATTGATAAATATGAAGATGGAAATCCTATATTTTTAGAAAAAAAACTGATAATAAAAATTGATGCTTATACAAAAATTAGAGATTATTTGAGCTATAAAGTCGGAGCCAATATTCTTATTAACTATGACTTAGCAAATAACCTATCAGATAATAGAAATATAGACTCAGATTTTAAAACAAGAACCCTAAAAAATATTGATATTTTTCTTACTGACAAGAGTTTTAAAGAAGATGTTAATAATTCTTCTGCAACTGTAGTTAAAGAATTTTTTGTTACATATTTTAAATTCAATCAGCTGAAACAAAATATGCCCTGA", "aa": "MYQIVVEKKANDILAKNYICEDVTFKISASKSLKSNTLVSNTMLKKYPIFIKINRDEKSNKLSITLSIHRLIYEVATKKEISINKKSLNLSIYHETNDEYIHFKIDKYEDGNPIFLEKKLIIKIDAYTKIRDYLSYKVGANILINYDLANNLSDNRNIDSDFKTRTLKNIDIFLTDKSFKEDVNNSSATVVKEFFVTYFKFNQLKQNMP", "aa_hexdigest": "6601fd43aad25577804d55cbd1c75720", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001E586E53", "query_cov": 1.0, "subject_cov": 0.8293650793650794, "identity": 0.986, "score": 403.0, "evalue": 2.8e-141, "valid": true, "uniref50_id": "UniRef50_UPI001E586E53" }, "pscc": { "uniref50_id": "UniRef50_UPI001E586E53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24452.90580000002, "isoelectric_point": 9.263155937194824 }, "id": "LMJBGEANGM_463", "locus": "GCA013368015_00439" }, { "type": "cds", "sequence": "contig_1", "start": 244056, "stop": 244319, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9", "UniRef:UniRef90_D1J7W9" ], "nt": "ATGCGTCGTAGTAGCAACAGAGCATTCTTTTTAAAATGTTTTAATTATTTTAAGGAAATAATAAAAGAGTTAAGGGAACGAAAAATTAAAATAGATATAAATAAAATTCCCGAAATTTTTAATAAAGAGAATTTAATTTCACTTAAATTCTTAATTCAAAAATATGTTTTAAAAACAGCAAAATTGTTTGAATCTTCATTGAGTGATGATTTGAAATGTATTTATATTAAATATTTTAAAGAACTTAAAGATGATATTAAGTGA", "aa": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKYVLKTAKLFESSLSDDLKCIYIKYFKELKDDIK", "aa_hexdigest": "8c8b63501e8df17ceedf37253533e467", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 243972, "stop": 244319, "observations": { "mutation": [ 243989, 244058 ], "stop": [ 243989, 244058 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W9", "query_cov": 1.0, "subject_cov": 0.7565217391304347, "identity": 0.9890000000000001, "score": 166.0, "evalue": 6.83e-52, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKNVLKTAKLFESSLSDDLKCIYIKYFKELKDDIKWTDFFFSKSSYYRKLNYLILAIAWFLIF" }, "paralog": false, "identity": 0.9739130434782609, "score": 218.0, "evalue": 1.4e-74, "description": "Internal stop codon at 243989, 244058. Nonsense mutation around 243989, 244058.", "psc": { "uniref90_id": "UniRef90_D1J7W9", "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W9", "UniRef:UniRef50_D1J7W9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10646.579699999987, "isoelectric_point": 9.666018867492678 }, "id": "LMJBGEANGM_464", "locus": "GCA013368015_00440" }, { "type": "cds", "sequence": "contig_1", "start": 244662, "stop": 244769, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAGATGATTGATGGTGGCGAAACAGATACTAAATTAATTGCAGTCCACGCAGATGATTATAGATTAGATAATATTAAATCATTGGCAGATGTCGATCAAATGTGA", "aa": "MKMIDGGETDTKLIAVHADDYRLDNIKSLADVDQM", "aa_hexdigest": "c59fcdc496f74d2b8de938ffed59a64d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 34, "aa_cov": 0.9714285714285714, "hmm_cov": 1.0, "evalue": 3.342077536206806e-09, "score": 32.26105499267578, "start": 1, "stop": 34 } ], "seq_stats": { "molecular_weight": 3923.4050000000007, "isoelectric_point": 4.351331901550293 }, "id": "LMJBGEANGM_465", "locus": "GCA013368015_00441" }, { "type": "cds", "sequence": "contig_1", "start": 244854, "stop": 245009, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAAATTTAGAAGTAAACATTGAAATTAGTAAAGATTCAAAAATCAAATATGAATATGACCGCAAAAGCAACAAAATCAAAGTTGACAGAATTTTAAGAGGTGATTTTAAATATCCTGCAAATTATGGCTATTTATCTGATGCACTAGATTGA", "aa": "MKNLEVNIEISKDSKIKYEYDRKSNKIKVDRILRGDFKYPANYGYLSDALD", "aa_hexdigest": "cc1867663a18a93573bdd0ed9b325cc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 2.783039116000496e-13, "score": 45.507972717285156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6058.826600000001, "isoelectric_point": 8.92521266937256 }, "id": "LMJBGEANGM_466", "locus": "GCA013368015_00442" }, { "type": "cds", "sequence": "contig_1", "start": 245138, "stop": 245410, "strand": "+", "frame": 2, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "SO:0001217", "UniRef:UniRef50_B3PMK3", "UniRef:UniRef90_D1J7X1" ], "nt": "ATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACTAACTATTTAATTTTAGATCATAATGGTGAGGGCGAATTGATATACGTGGCGGATATAAAAAGATTTAATAAGGATGATTTGAGAAAAATATTTATTTACGATGTGGTAAATGAATATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCTAAGACAGCAATTTCTCTTCTAAATTTAGGATGA", "aa": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLG", "aa_hexdigest": "6ab85d8a3108cf0df2cf20e20a0b18c2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ruvA" ], "truncated": "3-prime", "pseudogene": { "start": 245138, "stop": 245713, "observations": { "mutation": [ 245408 ], "stop": [ 245408 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X1", "query_cov": 1.0, "subject_cov": 0.4368932038834951, "identity": 1.0, "score": 183.0, "evalue": 2.72e-57, "gene_start": 1, "gene_end": 90, "reference_start": 1, "reference_end": 90, "reference_sequence": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLGWENLIQLIANEDKDKLCEASYVSLRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ" }, "paralog": false, "identity": 0.9947643979057592, "score": 371.0, "evalue": 8.08e-134, "description": "Internal stop codon at 245408. Nonsense mutation around 245408.", "psc": { "uniref90_id": "UniRef90_D1J7X1", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_B3PMK3", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X1", "EC:3.6.4.12", "UniRef:UniRef50_B3PMK3", "COG:COG0632", "COG:L", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK3" ], "product": "Holliday junction branch migration complex subunit RuvA" } }, "id": "LMJBGEANGM_467", "locus": "GCA013368015_00443" }, { "type": "cds", "sequence": "contig_1", "start": 245615, "stop": 245758, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07499.19" ], "nt": "ATGAGAATGCTCGGTTTTAAAAAACAACAAATTTCATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAG", "aa": "MRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ", "aa_hexdigest": "4d3fcfe438bc4408c6a51f35a9623a7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07499.19", "name": "RuvA_C", "length": 31, "aa_cov": 0.6595744680851063, "hmm_cov": 0.9354838709677419, "evalue": 1.4224689591483738e-06, "score": 24.261507034301758, "start": 3, "stop": 33 } ], "seq_stats": { "molecular_weight": 5473.3271, "isoelectric_point": 6.155799293518065 }, "id": "LMJBGEANGM_468", "locus": "GCA013368015_00444" }, { "type": "cds", "sequence": "contig_1", "start": 245733, "stop": 246686, "strand": "+", "frame": 3, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "RefSeq:WP_012855462.1", "SO:0001217", "UniParc:UPI0001BC2154", "UniRef:UniRef100_D1J7X2", "UniRef:UniRef50_D1J7X2", "UniRef:UniRef90_D1J7X2" ], "nt": "ATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAACAAATTGATCATTTACTATTTTACGGCCCGCCAGGTTTAGGAAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGCAATATTGTTTATGTTCAAGGTCCATTAATCGAAAAAAAGAGTGATGTATTGACTATTTTTTCTTCAATAAATGATAATGACATAATTTTTATTGATGAAATACATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAGGGTACAATTGACGTTGCCTTAGGGGTTGATGGTGATAAAAAAATCATGAGAATGAAATTAAAAAAATTTTGTTTGATTGCGGCCACGACAAAAATCAATTTGTTATCCAAACCCTTAAAAGATCGTTTTGGTTATATAGGAAAATTAAGCGATTATAGCGATTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATAAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTAGAAACTGTTAAGAAAGCCTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTACATAAAGTATTTAATGACAAATTTTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAA", "aa": "MSQEFSINSFDEFVGQEKLISTIKIIIESAKVQKKQIDHLLFYGPPGLGKTTLAKIISKETKSNIVYVQGPLIEKKSDVLTIFSSINDNDIIFIDEIHGINKNIEELFYSALEEGTIDVALGVDGDKKIMRMKLKKFCLIAATTKINLLSKPLKDRFGYIGKLSDYSDLEIAKIIFNSATKNKIKIKNDAINFIAIHSRQTPRVANTLLKRVNDFAIYYKIDLIDLETVKKAFSYLGIYLYGLYSPQIEYLKTLHKVFNDKFSSLDAISSIIKDDKYTILNDIEPILLIYKLIEKSPRGRKITNNGIEYLKSQNIIT", "aa_hexdigest": "2222fa95e3b47663b8bdd442e2d1d476", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2154", "ncbi_nrp_id": "WP_012855462.1", "uniref100_id": "UniRef100_D1J7X2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2154", "RefSeq:WP_012855462.1", "UniRef:UniRef100_D1J7X2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7X2", "uniref90_id": "UniRef90_D1J7X2" }, "psc": { "uniref90_id": "UniRef90_D1J7X2", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_D1J7X2", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X2" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "LMJBGEANGM_469", "locus": "GCA013368015_00445" }, { "type": "cds", "sequence": "contig_1", "start": 246848, "stop": 247402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAAACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGAGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGCGGTAATCCATTATTTTATAAAGGTGAATATGTTTCTAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTAAAAAATGGACCTCAAAACTACAATATTAGTGTAGAAAAAAATCCAGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACAGGCTTGCAACAAGTTGA", "aa": "MFPQLKKQSNSNVTSAKIGLKIERENQKENSYKKSYGLSDQEILNLTKNYIGNQNGVLSTSYDINLVSKNLISATSYQDKTDKDVNNLISNFVNKPYLTVTDGGGNPLFYKGEYVSRFYPNPDVPRTLKDFLKNGPQNYNISVEKNPATTYNKFGARQKIQVKLDEYGWDSFVQMINEYRLATS", "aa_hexdigest": "1ce245a0de981bfa4387d8bdadfc9cb8", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20994.258899999993, "isoelectric_point": 9.267217445373536 }, "id": "LMJBGEANGM_470", "locus": "GCA013368015_00446" }, { "type": "cds", "sequence": "contig_1", "start": 247861, "stop": 248466, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATATTACAAATATTGCAAAACCTATACTAAAATATTTTGATTTTGAGATTAAATCAATCTTTGCTATCAAAGAATTTGCTCTTTTGCTATTTCAAACAATTCAATTTGTTCTTTTATCAAACGTTTCTGTTTTTATTAAATTTCACATTATTAACGCATTGACCAATACTCCAATAATCAATCCAGAAACCAATCCAATAAATAGCATTATGCCCATCGCATTTAAATATGAAGTTTTTATAAAAATAGTAAATATTGAAACCACAATTAATGCAAAGGCAATATCAAGTGTTGATATTATTCCAAACCTTAATGATTTATTTGTTGCTTTTATTCCGTTAGAACCCGAATTTACTTCTTTATCTAAACGTCTTAATATTTGTCAAATCATTAAAAATGTTAAGAATGTAGAAACTAAGAAAGTAAATGCTACCGCTCCCGTTATATATATATTAAATGAAGTAATAATTGATAAAAACACAAATATCATAAATGCCAAGCATATTGATGATACGATTCCATATAAACGATATTTTGCAATCAAAAATACTGCAAACAATGAAAATAAAATTGCTAAAACTGCTAAATATCTATTTTGA", "aa": "MVNITNIAKPILKYFDFEIKSIFAIKEFALLLFQTIQFVLLSNVSVFIKFHIINALTNTPIINPETNPINSIMPIAFKYEVFIKIVNIETTINAKAISSVDIIPNLNDLFVAFIPLEPEFTSLSKRLNICQIIKNVKNVETKKVNATAPVIYILNEVIIDKNTNIINAKHIDDTIPYKRYFAIKNTANNENKIAKTAKYLF", "aa_hexdigest": "56bd7e673b64aa76d41dc0f614ac31af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22920.78330000003, "isoelectric_point": 9.301256752014158 }, "id": "LMJBGEANGM_471", "locus": "GCA013368015_00447" }, { "type": "cds", "sequence": "contig_1", "start": 248605, "stop": 249003, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATTGTCCATGGCAAATTATTAATCAAACTATTGAACTTATAACTGCTAATATTTCGTTTATTCACATTACAAAAAACGCTATTAAACCAAATCTTATAATGCTATATATTCCACAAATTATTAATGAAACCACAATTATTAAAATAGTAAATCCAAAATTGATTAATTCACCATTTGCATTTAATGATATTTCGTGTATGTTCAAATCAAATTCATTACCTATAGCTGAAATTTTAGTATTAATAAATGTCTTTAAAGCTTCTAATTTAGTTGAATCAATCTTGTCCGCTGAAGAAATTAATAAATTATATGAGTCGTTAGTTAAACTTGATTTTTCAAAGAAAATATTTGCATTTGAAATATTTAATTCTTTTCAATATTTAATAATTTGA", "aa": "MLNCPWQIINQTIELITANISFIHITKNAIKPNLIMLYIPQIINETTIIKIVNPKLINSPFAFNDISCMFKSNSLPIAEILVLINVFKASNLVESILSAEEINKLYESLVKLDFSKKIFAFEIFNSFQYLII", "aa_hexdigest": "80462d273146f0136045a8b9f01d3483", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15122.824199999985, "isoelectric_point": 6.558730888366699 }, "id": "LMJBGEANGM_472", "locus": "GCA013368015_00448" }, { "type": "cds", "sequence": "contig_1", "start": 249020, "stop": 249325, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAAATGACCAAATAAACAACATATTTAAAAAATATGTAAAGGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCTTCTACATATACATTGATGACGGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTACCAAACATTTGA", "aa": "MILSTAFSVVDSISNSQTIKEEFGNNLNTKNYIFENDQINNIFKKYVKESLNKNIFNIFFAIIFVASTYTLMTGFNATTILVTSMFIISNSFINLFILPNI", "aa_hexdigest": "d59ab9b7ad89d1f922ef06318eb63ce7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11525.187799999985, "isoelectric_point": 6.089752388000488 }, "id": "LMJBGEANGM_473", "locus": "GCA013368015_00449" }, { "type": "cds", "sequence": "contig_1", "start": 249457, "stop": 250632, "strand": "+", "frame": 1, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "SO:0001217", "UniRef:UniRef50_A0A3Q9VA72", "UniRef:UniRef90_D1J7X4" ], "nt": "ATGTTTCAAAGACTTAAAGGCACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTGATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAAGATAATGGCCAAAGAGACATTGCCTTGAGGCCAGAAGGAACAGCCTCAACCATAAGAGCTTTTGTGGAAAACAAAATAAACAATTTGGAGCCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGTCCTCAAAAAGGTCGCTATAGACAATTTATTCAAGGAGGAGTTGAATTAATTGAAAATTCAACTGTTGAGAATAATTTTGAAATAATAAAATTAGCATATGATTTTTTAAAGTCATTAAAAATTAGTAACATTACTTTAGAAATAAACAATCTGGGCTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAGGAATATTTTTCTAAATACAAGGAACAATTGAGCGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTGAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCTCCAAGATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAACAATTTACTAGATTTACTAGATGAATTTGAAATCAAATATGTAATAAATCCTTACTTAGTTAGGGGGCTTGATTACTACAATGATGTTGTTTTTGAATTTGTTTCAAATTCAGACGCTCTAGGTTCACAATGTACTGTTCTTGGCGGTGGAAGATATGATGGCATGATTAAAAGTTTTGGCGGACCTGATATAGGTTCTATTGGGTTTGCTTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAGTTTTAATGAAACGATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAGGAATTTTCAAGTAATTTTTCCAAGTCAAAAACAATCAATTAAGAGTTTGTTTAAACTTCAATATAAATTGCAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAATGAGTTGA", "aa": "MFQRLKGTRDIYGLEEKVLNLIENKFFKIIKNYNFKRICTPIIEDANLFIRSVGETSDIVSKEMYLFKDNGQRDIALRPEGTASTIRAFVENKINNLEPTKLAYFGPMFRYERPQKGRYRQFIQGGVELIENSTVENNFEIIKLAYDFLKSLKISNITLEINNLGSFESRNNYIEILKEYFSKYKEQLSEISLIRLQKNVLRILDDKEEIKKDFVKNAPRLIDYLSSIEKERFNNLLDLLDEFEIKYVINPYLVRGLDYYNDVVFEFVSNSDALGSQCTVLGGGRYDGMIKSFGGPDIGSIGFAFGVDRLMEIIMSNIDNYPELSFNETILIAYLNEDEKNEIRKIAYDLRRNFQVIFPSQKQSIKSLFKLQYKLQPKYLIFKELNMKKMS", "aa_hexdigest": "b1ee9d5e29f3cdc7678e2bc456f8fe61", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J7X4", "query_cov": 0.9948849104859335, "subject_cov": 0.9152941176470588, "identity": 1.0, "score": 762.0, "evalue": 2.42e-277, "valid": true, "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A3Q9VA72", "cog_id": "COG0124", "cog_category": "J", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3Q9VA72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VA72" ], "product": "Histidine--tRNA ligase" }, "genes": [ "hisS" ], "id": "LMJBGEANGM_474", "locus": "GCA013368015_00450" }, { "type": "cds", "sequence": "contig_1", "start": 250629, "stop": 250733, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAATAAAATCACTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAA", "aa": "MKIKSLLNERNIIYTNLDEFKNIISEMKRDENNE", "aa_hexdigest": "a7b6947067800c5975af138931c604c9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4156.694600000001, "isoelectric_point": 5.262347221374512 }, "id": "LMJBGEANGM_475", "locus": "GCA013368015_00451" }, { "type": "cds", "sequence": "contig_1", "start": 250858, "stop": 251469, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "GTGCAATTAATATTTCAAAATGTATCAGATCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAACAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTCGATAAACAAGGTTATATTGAAGTGGAAACCCCAATTTTAGGTAAATCAACTCCCGAAGGAGCTAGAGATTACTTGGTTCCTACTAGAAGAAAAGGAAGATTTTTTGCATTACCTCAATCACCACAACTATTTAAGCAATTATTGATGGCTGCTGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTTAGAGATGAAGATTTAAGAAAAGATAGACAACCAGAATTCACTCAATTAGATATTGAAATGTCATTTGGAAGTCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGA", "aa": "MQLIFQNVSDPLLTKESCIYVKGTVQKRLEVNPALKTGEIEVIVSDYKIFNSAKQIPFEINNSKSTNDANEDIRLEYRFLDLREERMLNNLRLRHKFLLLVRNFFDKQGYIEVETPILGKSTPEGARDYLVPTRRKGRFFALPQSPQLFKQLLMAAGFEKYFQIARVFRDEDLRKDRQPEFTQLDIEMSFGSQEEIFKLCEEM", "aa_hexdigest": "56df0164e538cf164b07c9a2d475a141", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_G9YIR7", "query_cov": 0.9310344827586207, "subject_cov": 0.8217391304347826, "identity": 0.556, "score": 193.0, "evalue": 7.66e-59, "valid": false, "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-" ], "uniref50_id": "UniRef50_A0A951N4R3", "go_ids": [ "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 136, "aa_cov": 0.6600985221674877, "hmm_cov": 1.0, "evalue": 1.4333616205289914e-60, "score": 200.42628479003906, "start": 70, "stop": 203 } ], "seq_stats": { "molecular_weight": 23943.272099999984, "isoelectric_point": 6.65228786468506 }, "id": "LMJBGEANGM_476", "locus": "GCA013368015_00452" }, { "type": "cds", "sequence": "contig_1", "start": 251488, "stop": 251808, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGGTACAAAATTGAAACTCCATTTCCAAAAATGGACTTTTTCTATTCAATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATTGAAGATTATTCAAAACAATTTGCGGCCAAATATAATAAGAAATTTGTAAAAGCTATTGTTTTTGACAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTAGACATATTCGACTTAAAAGAAGCAAACCAAAATGAATTCCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGA", "aa": "MGYKIETPFPKMDFFYSMAHYGNDKPDTRFGFLIEDYSKQFAAKYNKKFVKAIVFDRNINEHKQIINETFKKNNGQILDIFDLKEANQNEFQCLLDKAKEDNISTP", "aa_hexdigest": "369ba5b3bcb30183525d491ce220a7a8", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12543.092099999985, "isoelectric_point": 6.724530220031738 }, "id": "LMJBGEANGM_477", "locus": "GCA013368015_00453" }, { "type": "cds", "sequence": "contig_1", "start": 251941, "stop": 252411, "strand": "+", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "nt": "ATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATACGATTTAGTGCTAAATGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTTGGATTCTTTTTAAAAGCATTTGAATATGGTCTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATCAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGTATTGACTTGTTGACATCAGCTCCTAGCGATGTGACAAAAGAACAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAA", "aa": "MFEYDRETKTYSPAHHAFTQFEESTMKYLETGELEKVRAKSYDLVLNGFELGSGSIRIYDPKIQKKMFDVINLSEKQQQDRFGFFLKAFEYGLPPHNGIAFGVERILMILTKSESIRDVIAFPKNAKGIDLLTSAPSDVTKEQLDEYGLELKINKK", "aa_hexdigest": "f007a3977b2685b4435b15c340846545", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021FB0CEA", "query_cov": 0.9743589743589743, "subject_cov": 0.8837209302325582, "identity": 0.5660000000000001, "score": 176.0, "evalue": 1.44e-53, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0021FB0CEA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FB0CEA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "LMJBGEANGM_478", "locus": "GCA013368015_00454" }, { "type": "cds", "sequence": "contig_1", "start": 252504, "stop": 252683, "strand": "+", "frame": 3, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_I5D5Q9", "UniRef:UniRef90_D1J7X6" ], "nt": "ATGCATGCTGCTTCAATAGCCTTAACAGTTATATTGGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGCGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATTAAAAAAGTATTAAAGTGA", "aa": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLK", "aa_hexdigest": "287b3e133ec6143731f4c8470f1a19f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secG" ], "truncated": "3-prime", "pseudogene": { "start": 252504, "stop": 252755, "observations": { "mutation": [ 252681 ], "stop": [ 252681 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X6", "query_cov": 1.0, "subject_cov": 0.7108433734939759, "identity": 1.0, "score": 105.0, "evalue": 2.03e-28, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLKWSMITLGILLFIFLIALRVVIQNG" }, "paralog": false, "identity": 0.9879518072289156, "score": 151.0, "evalue": 3.09e-49, "description": "Internal stop codon at 252681. Nonsense mutation around 252681.", "psc": { "uniref90_id": "UniRef90_D1J7X6", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_I5D5Q9", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X6", "UniRef:UniRef50_I5D5Q9", "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5Q9" ], "product": "Protein-export membrane protein SecG" } }, "id": "LMJBGEANGM_479", "locus": "GCA013368015_00455" }, { "type": "cds", "sequence": "contig_1", "start": 252771, "stop": 253661, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "ATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCTAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTTTTTAGTGCCTTTCTTGCACCTTTTCAATTAAAAGCGGTATTAGACAAGGATTTATTGCTTGCAAATATTTATTATTATTATGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGCAAGAAAAACTATTGTTGGAACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGTGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAAAATAATTGA", "aa": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNN", "aa_hexdigest": "b84779a2db2508ed1bd633966b9eb56a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "3-prime", "pseudogene": { "start": 252771, "stop": 253964, "observations": { "mutation": [ 253659 ], "stop": [ 253659 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.41283124128312415, "identity": 1.0, "score": 570.0, "evalue": 5.8e-199, "gene_start": 1, "gene_end": 296, "reference_start": 1, "reference_end": 296, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9974811083123426, "score": 782.0, "evalue": 1.61e-284, "description": "Internal stop codon at 253659. Nonsense mutation around 253659.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "LMJBGEANGM_480", "locus": "GCA013368015_00456" }, { "type": "cds", "sequence": "contig_1", "start": 253776, "stop": 254924, "strand": "+", "frame": 3, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "ATGTTGCCGGTTGAATTGTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAACAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTAAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAACGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATTGGACATTTCGGTTTAGCTTCAAAAGCTTACTCGCATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTGATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCTGATTTATCAGAAACGCTAAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGTGAATCTGAAAAAAATGCAATGGTTGTTGAACGAAGTGTTGTTGATGTTAGAAAATCGGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAATATTGATGAATACCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTTGCGATTCCAACTAATAAAGACAACGATTTTCAAGCATCATCTAAAAATAAAATCTATAAAGCAGGCAATAAGTATTTAATTACTATCACTTCGATAGATCACGAAAAAGGAAATGTAAATGCAACATTAGCTTAG", "aa": "MLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA", "aa_hexdigest": "394fc5c8fda9b00101a545e1534b38ec", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rnr" ], "truncated": "5-prime", "pseudogene": { "start": 253476, "stop": 254924, "observations": { "mutation": [ 253659 ], "stop": [ 253659 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.5327754532775453, "identity": 1.0, "score": 741.0, "evalue": 1.12e-264, "gene_start": 1, "gene_end": 382, "reference_start": 336, "reference_end": 717, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9979253112033195, "score": 945.0, "evalue": 0.0, "description": "Internal stop codon at 253659. Nonsense mutation around 253659.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "LMJBGEANGM_481", "locus": "GCA013368015_00457" }, { "type": "tmRNA", "sequence": "contig_1", "start": 255425, "stop": 255795, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 255508, "stop": 255585, "aa": "AEEKQNKQSFVLNQMMSSNPVFAY", "nt": "GCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAA" }, "nt": "GGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAAACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCTAATGAATAGCTCGGTTCATTGCTAGCCTTTGGAGAGCTCAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGATTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAATGAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCA", "id": "LMJBGEANGM_34", "locus": "GCA013368015_00458" }, { "type": "cds", "sequence": "contig_1", "start": 256144, "stop": 257067, "strand": "+", "frame": 1, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "RefSeq:WP_224154276.1", "SO:0001217", "UniParc:UPI00031D8762", "UniRef:UniRef100_D1J7X9", "UniRef:UniRef50_D1J7X9", "UniRef:UniRef90_D1J7X9" ], "nt": "ATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAAAAGTTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATCGCTGAAGCAATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTACTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGCAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCACAAAAGACCCAATCACATACGACCAAGTAAAACCTGGGGCGGTTATTATCGATGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAATGAATTTTCTAATGTTGCCTCATTTATAACCCCTGTTCCCGGAGGGGTTGGTCCAATGACTATTTCGGCATTGCTCATTAATCTTTTAAAAGCAAGTTCCTTGCAAAATCCAATTATTGTACCATATCTTAAATTTTTAAAATCATATTTTAATTAA", "aa": "MFSLKLGSLESSQAKMAILLNGKAISLKVKEDLKKAIEKMPQEIRPTLGILQVGDLSESNIYVKNKLKYASDIGLNTKLIKLQQNASKLQIAEAIELLNKQSTGFIVQIPMQTNQIIDSQEILDLISIDHDIDGLTSFNLDLKNPNNYFLSATASGIFLLLNKYNILYKNAKIGVIGQSKIVGYPLANFLVLKGYNVFRYNKDTPKNNIKDLDIIIVATGTKDPITYDQVKPGAVIIDVGIHRDSNNKISGDLNFNEFSNVASFITPVPGGVGPMTISALLINLLKASSLQNPIIVPYLKFLKSYFN", "aa_hexdigest": "e22028dad75412158aadf0e3c089fb52", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00031D8762", "ncbi_nrp_id": "WP_224154276.1", "uniref100_id": "UniRef100_D1J7X9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031D8762", "RefSeq:WP_224154276.1", "UniRef:UniRef100_D1J7X9" ] }, "ips": { "uniref100_id": "UniRef100_D1J7X9", "uniref90_id": "UniRef90_D1J7X9" }, "psc": { "uniref90_id": "UniRef90_D1J7X9", "gene": "FolD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_D1J7X9", "cog_id": "COG0190", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_D1J7X9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X9" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "LMJBGEANGM_482", "locus": "GCA013368015_00459" }, { "type": "cds", "sequence": "contig_1", "start": 257167, "stop": 257304, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATAGGTGTTAGTGGTATGATTGCTGCCGGTAAAAGTTCATTAGCTGAAAAACTTCATCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGACGACGAAGTATTTAATACATTTTTGAAATGA", "aa": "MIIGVSGMIAAGKSSLAEKLHRYFEGSLMLHEFEEDDEVFNTFLK", "aa_hexdigest": "0bfc1de864416f9cb21613380c6e5897", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.789099999999, "isoelectric_point": 4.8198556900024405 }, "id": "LMJBGEANGM_483", "locus": "GCA013368015_00460" }, { "type": "cds", "sequence": "contig_1", "start": 257428, "stop": 257646, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01712.25" ], "nt": "ATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTAATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATATTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGGCAAAGTGAAGTAGATAACTGA", "aa": "MIFLDRFSIEHYIFAKLILSKKGKRYLDAYDALFEKLISKDELPDFAIFLDINFETFKKRIFTRGRQSEVDN", "aa_hexdigest": "579e8b1a95c98a26c99315edf116751f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01712.25", "name": "dNK", "length": 62, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 1.1129932099160504e-07, "score": 27.327556610107422, "start": 10, "stop": 69 } ], "seq_stats": { "molecular_weight": 8669.956499999993, "isoelectric_point": 7.985392570495606 }, "id": "LMJBGEANGM_484", "locus": "GCA013368015_00461" }, { "type": "cds", "sequence": "contig_1", "start": 257958, "stop": 258305, "strand": "+", "frame": 3, "gene": null, "product": "Hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "nt": "TTGCCATTTTTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTGGATTGCATGATAACTAGCTCATATGATGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGATTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCGCTGCTAAAATCAAGAAATCCTAAATCCCTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTGGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAAACGACTTTGTTGTTGGCTTTGGCTTCGATTGA", "aa": "MPFLAQLIKDVTVDFVLDCMITSSYDGKDRSSGNVKITLDLQNNIADKDVLIVEDIVDSARTMTKVVSLLKSRNPKSLKVLTLLDKPSGRLVEFKQDNFGFEIGKNDFVVGFGFD", "aa_hexdigest": "07aa8cd7bb537509898835ce21ef9a5d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PE13", "query_cov": 0.991304347826087, "subject_cov": 0.8085106382978723, "identity": 0.535, "score": 116.0, "evalue": 3.98e-31, "valid": false, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_A0A3B0PE13", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PE13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PE13" ], "product": "Hypoxanthine phosphoribosyltransferase" }, "genes": [], "id": "LMJBGEANGM_485", "locus": "GCA013368015_00462" }, { "type": "cds", "sequence": "contig_1", "start": 258391, "stop": 258747, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2", "UniRef:UniRef90_D1J7Y2" ], "nt": "ATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGCATGCCTTTAGCTATTGGCTCTGCGTTTGTGGCATTTATTTTAATAGTTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAATCAAAAACAAGAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAATTAAATTCCTTAAAAATTGAAAAACAAATCGCTTACTTATTTGACTCGCAAAAATTTTTTAAAGAAAATTTAAATAATTTATGA", "aa": "MILKLQNWQITETSNQDSGTSMPLAIGSAFVAFILIVAVVIFIIKKIINKQSKTRDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDSQKFFKENLNNL", "aa_hexdigest": "c7edfe395a298bdb7afa6704e7f9098f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 258334, "stop": 259050, "observations": { "mutation": [ 258343, 258745, 258865 ], "stop": [ 258343, 258745, 258865 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y2", "query_cov": 1.0, "subject_cov": 0.4229390681003584, "identity": 0.9490000000000001, "score": 210.0, "evalue": 1.93e-66, "gene_start": 1, "gene_end": 118, "reference_start": 20, "reference_end": 137, "reference_sequence": "MVYWQIKKLINTKNTNNFSIILKLQNWQITETSNQDSSTSITLAIGSAFVAFILIAAVVIFIIKKIINKQSKTRDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDPQKFFKENLNNLWVKKYYEIIKKFKLEKSPTSKFNKLKINKYYKNYLNEVGQWNYCRHHIDEIKISGAIYSAHPQTKEYYKTGDVILVTYEQHLFLHYLIVMAKTTMPNHGMIVGLKFHFNDTKKCIEYWDKVIQKMCEKYLVKYDKNWSENLT" }, "paralog": false, "identity": 0.9537815126050421, "score": 453.0, "evalue": 1.74e-164, "description": "Internal stop codon at 258343, 258745, 258865. Nonsense mutation around 258343, 258745, 258865.", "psc": { "uniref90_id": "UniRef90_D1J7Y2", "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y2", "UniRef:UniRef50_D1J7Y2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13672.652799999976, "isoelectric_point": 9.455658531188966 }, "id": "LMJBGEANGM_486", "locus": "GCA013368015_00463" }, { "type": "cds", "sequence": "contig_1", "start": 259891, "stop": 260688, "strand": "+", "frame": 1, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_012855474.1", "SO:0001217", "UniParc:UPI0001BC2196", "UniRef:UniRef100_D1J7Y4", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_D1J7Y4" ], "nt": "ATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGTTCATCGAAGCTGGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGTCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACAGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAGGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTCGACGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATAAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGTGGTCATGTTATTGCTCAAGGCAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAACCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAA", "aa": "MIEIKKISYKYPGSSKLALENIDLKIEEGQYIAILGHNGSGKSTFSKLLSAIYKPTSGKIIIDGLEINSENLLEIRKKIGIVFQNPDNQFIGSTVEDDIAFGLENKMLKPEEMKTIIYKYAQEVGMLDQLDREPQNLSGGQKQRVAIASTLALDPKIIIFDEITSMLDPKGRNDIYKIIHNLHKKTNKTLISITHDMDEALLADKLIVFAGGHVIAQGKPIEILNNKEIIEIAKIDSPFIYKLSSLLNDIEPTYNREELIKEICK", "aa_hexdigest": "5a3ed23426316973a47bd29793f9d158", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC2196", "ncbi_nrp_id": "WP_012855474.1", "uniref100_id": "UniRef100_D1J7Y4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2196", "RefSeq:WP_012855474.1", "UniRef:UniRef100_D1J7Y4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Y4", "uniref90_id": "UniRef90_D1J7Y4" }, "psc": { "uniref90_id": "UniRef90_D1J7Y4", "gene": "EcfA2", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA2" ], "id": "LMJBGEANGM_487", "locus": "GCA013368015_00464" }, { "type": "cds", "sequence": "contig_1", "start": 260679, "stop": 260978, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6", "UniRef:UniRef90_UPI001E584429" ], "nt": "ATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCCATTATTGGCCCAACTGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTACAACTGGAAAAATTATCTTTAATGAGTTGCCAATAAAAATTAAAACTCCAAAACCTAAAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGA", "aa": "MQIKASEITKEYDKGLPTYIKVIDNVSLEISEGEAISIIGPTGSGKTTLIEHFNGLIIPTTGKIIFNELPIKIKTPKPKKPNLKKIKHDSNEYEIVLEK", "aa_hexdigest": "a7930c79e4274b359921dd240c9f5d34", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI001E584429", "query_cov": 0.8484848484848485, "subject_cov": 0.9545454545454546, "identity": 0.988, "score": 164.0, "evalue": 2.81e-51, "valid": true, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A448ZZL6" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6" ], "product": "Cobalt ABC transporter ATP-binding protein" }, "genes": [], "id": "LMJBGEANGM_488", "locus": "GCA013368015_00465" }, { "type": "cds", "sequence": "contig_1", "start": 261102, "stop": 261476, "strand": "+", "frame": 3, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "SO:0001217", "UniRef:UniRef50_Q4A5A4", "UniRef:UniRef90_UPI0019672E9D" ], "nt": "GTGGGAGTTGTATTTCAATTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGATGAGGCTAAAAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGATTATTTGCAAAGAAGTCCATTTAATTTATCAGGCGGTCAAAAACGTAGAGTTGCGTTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAACCGACTGCCGGGCTTGATCCTCAAGGTGTCGAAGAAATGTTAAACTTATTTCACAATCTCTATTTAAATGGAAAAACGATCATTATTGTAACGCATGACCTTGATAATGCTCTAAAATGA", "aa": "MGVVFQFAEYQLFESTIEKDIIFGPISMGVSKDEAKKLALKYIEMVGLPIDYLQRSPFNLSGGQKRRVALAGILAMEPKFLVLDEPTAGLDPQGVEEMLNLFHNLYLNGKTIIIVTHDLDNALK", "aa_hexdigest": "9f34442d53614fac91ff9a4b5cd8ea2d", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 260802, "stop": 261677, "observations": { "mutation": [ 260976, 260997, 261030, 261474 ], "stop": [ 260976, 260997, 261030, 261474 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019672E9D", "query_cov": 1.0080645161290323, "subject_cov": 0.40584415584415584, "identity": 0.8240000000000001, "score": 202.0, "evalue": 6.19e-63, "gene_start": 1, "gene_end": 124, "reference_start": 109, "reference_end": 233, "reference_sequence": "MQIKVEKISKEFDKKLPTHIQVLKDISVDIKEGEIISIIGHTGSGKTTFIEHLNALIIPDSGKITFSNVDVNKKQWYQSKNKLQLVKMDIVVEKTKRKIKNIKSLRKEVGVVFQFAEYQLFESTIEKDIIFGPVSMGVPVEEAKKLAKKYIKMVGLPESYLQRSPFNLSGGQKRRVALAGILAMQPKYLILDEPTAGLDPAGVEQMLQLFANLNKEEGKTVIIVTHDLDNALRWTKRCILFKNGEIIRDGETYEVLNDEQCLIENKLRATQLLTFVNKLKARGVKIGKVTDINQLAEQINNWKQNKGV" }, "paralog": false, "identity": 0.613013698630137, "score": 329.0, "evalue": 5.2e-115, "description": "Internal stop codon at 260976, 260997, 261030, 261474. Nonsense mutation around 260976, 260997, 261030, 261474.", "psc": { "uniref90_id": "UniRef90_UPI0019672E9D", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q4A5A4", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019672E9D", "UniRef:UniRef50_Q4A5A4", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5A4" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" } }, "id": "LMJBGEANGM_489", "locus": "GCA013368015_00466" }, { "type": "cds", "sequence": "contig_1", "start": 261540, "stop": 261716, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGACGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCATTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAAGATTTAGCTGAAAAAATTAATTATTACTTAACCATGAAGCGCAAAGGAGGAACAAATGAATAA", "aa": "MSDDNFLIENNLIPTRLLSFVNELKKNNVDVGKVTSVEDLAEKINYYLTMKRKGGTNE", "aa_hexdigest": "223bb9badab2c74b88c13a5afb447dfe", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.472400000002, "isoelectric_point": 5.315036964416504 }, "id": "LMJBGEANGM_490", "locus": "GCA013368015_00467" }, { "type": "cds", "sequence": "contig_1", "start": 261709, "stop": 261876, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACGTTTATTCACAAAATTGATCCTCGTTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGA", "aa": "MNKTIIGKYLNYDTFIHKIDPRFKFLTNVLLIILLFMADHFITLGILIFSIMIFY", "aa_hexdigest": "4d86250178570e8cd34577cd44f37af2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6584.976999999995, "isoelectric_point": 9.224603843688964 }, "id": "LMJBGEANGM_491", "locus": "GCA013368015_00468" }, { "type": "cds", "sequence": "contig_1", "start": 261919, "stop": 262083, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGAGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCACTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGA", "aa": "MPLYIAIFLLIINMFTVKGAVSLNSVLSYVPLASDGNLDSYVYYRNETIINTIY", "aa_hexdigest": "9136553ba1c81ceac21472dfcb43c710", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6108.0802, "isoelectric_point": 4.556577491760254 }, "id": "LMJBGEANGM_492", "locus": "GCA013368015_00469" }, { "type": "cds", "sequence": "contig_1", "start": 262189, "stop": 262542, "strand": "+", "frame": 1, "gene": null, "product": "energy-coupling factor transporter transmembrane component T", "db_xrefs": [ "KEGG:K16785", "SO:0001217", "UniRef:UniRef50_UPI002FE370B7", "UniRef:UniRef90_UPI002FE370B7" ], "nt": "TTGACAGTTTCAACAAAACCAGTCTTACTAACCAGAGCAATTAATGATATTTTAATCCCTTTAAAGTTAGTTAAAATTCCTACAGAAATTATAACAATGATTATTTCAATATCATTACGTTTTATCCCAACATTGCTTGAAGAATCCAGTCGTATCATGAAAGCACAAGCAAGTAGAGGTATTGATTTTAAAAATGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCTAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAGAGATCTCACTATAGAGTGTTGACTCCTTGTTGA", "aa": "MTVSTKPVLLTRAINDILIPLKLVKIPTEIITMIISISLRFIPTLLEESSRIMKAQASRGIDFKNGNLKDKTKSFIVLIVPLFVSSFSKANDLADAMTSRGYEPYAKRSHYRVLTPC", "aa_hexdigest": "7a3e5d1e2d1f7a0dd421c5274039d566", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 262003, "stop": 262656, "observations": { "mutation": [ 262081, 262540, 262627, 262630 ], "stop": [ 262081, 262540, 262627, 262630 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE370B7", "query_cov": 0.9743589743589743, "subject_cov": 0.5089285714285714, "identity": 0.851, "score": 187.0, "evalue": 4.37e-58, "gene_start": 1, "gene_end": 114, "reference_start": 70, "reference_end": 183, "reference_sequence": "MPPEGTPYIPLAGEANIDTYVYHRGQTIINDIYWAPFGLNAKFQLTKFTILRTVSILFRIYGVILTTTILTVSTKPVFLTRAINDILYPLKLIKFPTEIITMIISIALRFIPTLLDEANRIMKAQASRGVDFKHGKLKDKTKAFIVLIVPLFVASFSKANDLADAMTSRGYDPYAKRSKYRQLGINWRDILSIFVIFGIVAMVTVCQIHSVHLPEWWIMTFHKV" }, "paralog": false, "identity": 0.7142857142857143, "score": 301.0, "evalue": 1.36e-105, "description": "Internal stop codon at 262081, 262540, 262627, 262630. Nonsense mutation around 262081, 262540, 262627, 262630.", "psc": { "uniref90_id": "UniRef90_UPI002FE370B7", "product": "energy-coupling factor transporter transmembrane component T", "uniref50_id": "UniRef50_UPI002FE370B7", "kegg_orthology_id": "K16785", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE370B7", "UniRef:UniRef50_UPI002FE370B7", "KEGG:K16785" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE370B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE370B7" ], "product": "energy-coupling factor transporter transmembrane component T" } }, "id": "LMJBGEANGM_493", "locus": "GCA013368015_00470" }, { "type": "cds", "sequence": "contig_1", "start": 262880, "stop": 264352, "strand": "+", "frame": 2, "gene": null, "product": "DNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "nt": "TTGGCTTCCGATCTATTTAAAAAAGTAAAACATAAACGTCCGATGCTTTCATTAAATAAAGCATATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACGTCTGATTTTTCTTTTTTTATTGAGCCTAAAATTGATGGCCTTTCAATTTCTATTATTTACGAAGATGGCAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTATTCAAATGTCTTGCATTCCTAAAACTATTAAATATAAGAAATCATTAGAGGTAAGAGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGGCAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCAGCAGCAGGCACATTAAGGCAATTAAATTCTCAAATTATTAAAGAACGCAATTTGTCAGCATTTTTGTATTATGTTGTAGATCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAATTCTTAAAAACTTATGGGTTTCCCGTTACTCCCGAAGTTCAACATGCTAATACATTGCAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAATTATTAATAGATTATGAAACAGATGGAGTTGTTATAAAGTTAAACGAGGTTCAATATTTTGACAAGTTGGGATCAACAGCTAAGTTCCCCCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATATGCGACAGTAGGAAGAACTGGATTGGTTACATACAATGCAGAATTAGAACCTGTAGAAATTTCGGGTAGTCTTGTAAGCTTTGCAACGTTGAATAATTTCCAATACATAAAAGATTTGAATTTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAACAATTTTGCTCAAATGATCAATGCCCTGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAGGATGGTTTAGATATTAATACTATGGGTGAGAGAAATATTGATCTATTTTTAAAAAATTCTTTAATTTCCAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAGTCTATTGAGAATATCTTAATTTCTATTGATGAATGTAAGAATAAACCTTTATATAAAATGATTTCTGCTCTTTCAATCCCACTAATTGGTGTAAAATTAGCTAAATTCGTAGCCTCAAAAATTAAATCGTTACATAATTTATTAAATTATGATTTTTCACAATTAATTGATTATCATGAAATAGGAGATAAAATTATAAATTCTATAATTTCTTGA", "aa": "MASDLFKKVKHKRPMLSLNKAYSLDEIQKFIDNIKKITSDFSFFIEPKIDGLSISIIYEDGKLVRAITRGDGITGEDVTANVIQMSCIPKTIKYKKSLEVRGEVYLAISDFEKLNLKLGQENKNKLANPRNAAAGTLRQLNSQIIKERNLSAFLYYVVDPLQHNIKTMQEEHEFLKTYGFPVTPEVQHANTLQDIEEYIRNFKNQKLLIDYETDGVVIKLNEVQYFDKLGSTAKFPHYAIAFKYEPNVETTTIKRIYATVGRTGLVTYNAELEPVEISGSLVSFATLNNFQYIKDLNLNENDLVYIKKAGEIIPCVIGLISGKKEIDHFQKVEFCPYCHNKLVDSETGLEQFCSNDQCPEIQRRKLIHFCSKDGLDINTMGERNIDLFLKNSLISNPIDIFNLKNKKQDLLKLNRMGEKSIENILISIDECKNKPLYKMISALSIPLIGVKLAKFVASKIKSLHNLLNYDFSQLIDYHEIGDKIINSIIS", "aa_hexdigest": "2a9e0e3723d4802c0bfe5248092f65bb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDB6CC", "query_cov": 0.9938775510204082, "subject_cov": 0.8513986013986014, "identity": 0.604, "score": 606.0, "evalue": 9.78e-212, "valid": false, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0AAJ5IJW6", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5IJW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "product": "DNA ligase" }, "genes": [], "id": "LMJBGEANGM_494", "locus": "GCA013368015_00471" }, { "type": "cds", "sequence": "contig_1", "start": 264443, "stop": 264664, "strand": "+", "frame": 2, "gene": null, "product": "BRCT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "nt": "TTGGAAAATAAATCATTTGTAATAACAGGAACATTAGGACATCCTAGGTCATATTTTGAAAGGTTAATTGAAAAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAATGCCGGATCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAA", "aa": "MENKSFVITGTLGHPRSYFERLIEKNGGLIHSSISSKTNYLLAGENAGSKLQKANKLNITIITEEDLFKMIGQ", "aa_hexdigest": "d4d32fb3645a81df0234ea56fb334690", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE292E9", "query_cov": 0.9863013698630136, "subject_cov": 1.0, "identity": 0.667, "score": 95.5, "evalue": 1.75e-24, "valid": false, "product": "BRCT domain-containing protein", "uniref50_id": "UniRef50_UPI002FE292E9" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE292E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "product": "BRCT domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_495", "locus": "GCA013368015_00472" }, { "type": "cds", "sequence": "contig_1", "start": 264641, "stop": 265288, "strand": "-", "frame": 2, "gene": null, "product": "MAG0480 family ComEC-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8", "UniRef:UniRef90_D1J7Y8" ], "nt": "TTGAATTCAAAGGAATTGGTTGTTGATTTGATTTCAATATTAATGTTATTTTTTTACCTAAAGTTATTAAATTTTTCAATATCTGCAAGTCGGGCGTTTTTGTTTTTGATATTAAAAATTGCAAGCAAAAACATTTTTAAAGGCAAATTTAATAACATTGACATATTGAGTTTTGCAGCACTATTATTGATATTCTTTGATATTAGAACCATGTATTCTTATTCTTTTATTTTATCTTTTACAATAACATTGATTATTTTTTTAATTTTAGACATTAAAAAAATTTACAAAAACAATATATTTGTAAATTTTCTATTTATTATATTACCTTATATTTCTTCTATTTCAATAGTAATTTCATTTAATCCAAAATTTAACATTTTAGGAATATTTTATCAATTATTCTTTCTACCAATTTCAATATTTAACTATGTATTTTGTTTATTTGCTTTTATAGTTAAACCTATAATAAAAATGTATGTTTCAATATTTGAAATAGTAATAAATACTTTAAGCTATGGTTGTTTGATTATTGAAGTAGAAAAAAATTCAATATTAGGATATTCTATTAATTTTTATATTATTTCAATATATACATATATTAAAATAAAGAGAGAATACTATTTATTGACCAATCATTTTAAATAA", "aa": "MNSKELVVDLISILMLFFYLKLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIVINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK", "aa_hexdigest": "031b8f30e2512643325163d50d584bbb", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 264641, "stop": 265588, "observations": { "mutation": [ 265291 ], "stop": [ 265291 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y8", "query_cov": 1.0, "subject_cov": 0.4725274725274725, "identity": 0.991, "score": 379.0, "evalue": 6.95e-129, "gene_start": 1, "gene_end": 215, "reference_start": 241, "reference_end": 455, "reference_sequence": "MTGIGKFTRSRWKNFNYIKKSFNNLNTNLFVALSFCLYCLIIYKINIVISSIMLSLIIAVNFFNLKYWIKIAIVFPLIFLVHYLWYLDLFKINGKNICGKFEIKRFFNKSILIKYQYYNVLVKNWSSWEFNNFYKYNIELSGTLSIIKNQNMFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILKWLNSKELVVDLISILMLFFYLKLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIIINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK" }, "paralog": false, "identity": 0.9936507936507937, "score": 603.0, "evalue": 1.41e-219, "description": "Internal stop codon at 265291. Nonsense mutation around 265291.", "psc": { "uniref90_id": "UniRef90_D1J7Y8", "product": "MAG0480 family ComEC-like protein", "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y8", "UniRef:UniRef50_D1J7Y8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "LMJBGEANGM_496", "locus": "GCA013368015_00473" }, { "type": "cds", "sequence": "contig_1", "start": 265289, "stop": 265591, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGGCACTTTATCAATAATTAAAAATCAAAATATGTTTAGTTTGAGCAATAAAATCGATTTTGAATTGTTAAATGTTAAATTGGTTTCAAAAGCATATGCATATCAAGAAAAAATTCCAATATTGCAAGATTATGATTATAAAACAAAAAAATTTCTAGCACTGGTTTTATTCAACATTCAACAAGCTAATGATTTGTTTGTAGATCAATTAAAACAACTAAGTGTTATGCACTATTTTACAATAAGCGGATTTCATTTTGGAATAATATATTTGCTTATATCAAAAATTTTAAAATGA", "aa": "MSGTLSIIKNQNMFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILK", "aa_hexdigest": "969b1b66faf6093a59ac9231443b9218", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11657.655199999992, "isoelectric_point": 9.331943702697757 }, "id": "LMJBGEANGM_497", "locus": "GCA013368015_00474" }, { "type": "cds", "sequence": "contig_1", "start": 266005, "stop": 266445, "strand": "-", "frame": 3, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9", "UniRef:UniRef90_D1J7Y9" ], "nt": "ATGAATAAACGAAAAATTTTTTTGGGACTAGGATTATTTTCATCAGCATCAATAATAATACTAATGTCAATAGGAATTAATAAATACATTTCAAATATAAAAAATAACAATAAATTATTTATCAAAAACAAAAATGAAATAATAACAATAGGAATATCGGGTGCAATAGAATATCCCGACTCATACGAAATTAAAAAAGGCACTAAATTATTGGAAATAATAAAACAAGCACATCTTAAAGCGGGCGCAGATTTAAACAATATTGATTTAAATTTAAAATTAGAAGAAAATCAATCAATTAATATTCCTTTTAAAAAAGATAATTCAATTTCTATTAGAGATGTCAATAACGTTGGCATATTAGAGGATTTAGGAATAAAAAAAGATATAGCAAAAATTATTTTAAGCTACTTCATTGCCAAGGATTATTTAATAACATGA", "aa": "MNKRKIFLGLGLFSSASIIILMSIGINKYISNIKNNNKLFIKNKNEIITIGISGAIEYPDSYEIKKGTKLLEIIKQAHLKAGADLNNIDLNLKLEENQSINIPFKKDNSISIRDVNNVGILEDLGIKKDIAKIILSYFIAKDYLIT", "aa_hexdigest": "ef19c3bb0aacf340f1382f6e0f07f785", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_D1J7Y9", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.9790000000000001, "score": 265.0, "evalue": 4.44e-89, "valid": true, "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_D1J7Y9" }, "pscc": { "uniref50_id": "UniRef50_D1J7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9" ], "product": "ComEA family DNA-binding protein" }, "genes": [], "id": "LMJBGEANGM_498", "locus": "GCA013368015_00475" }, { "type": "cds", "sequence": "contig_1", "start": 266586, "stop": 267227, "strand": "-", "frame": 1, "gene": "tACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "COG:COG0217", "COG:KJ", "SO:0001217", "UniRef:UniRef50_Q98PU5", "UniRef:UniRef90_D1J7Z0" ], "nt": "ATGTTTCAAAAATTTTCAAAAGAAATTATTGTTGCTGCAACATTAGGCGGACCAGATCCAGACTCTAACCCATCGCTAAAATTAGCAATTGCTAAGGCTAAGGCAAAAAGTATGCCTAAAGCAAACATTGAAAAGGCAATTGCAAAAGTAGCTGGCGGATCTAAAGAAGCTGCAAACTATCAATCTTATTTATATTCAGGTACAGCATTTGGCGGTGTAAATTTTATTGTAAGTTGTTTAACAGATAATTTCAATCGTTTAAGCTCGAATATCAAACATTTCTTTTCAAAACACAATGGTCAATTAGGAAAACAAGGAATAATACCATATGTTTTTGATCAAAAAGGTTTAATTGAATTTCCAAGTAATGTTTCTGGTGAAGAAGAAACCATGATGACAGTTTTAGATGCGGGAGCAGAAGATTTTAGAAATGAAGATGGAACTTATACAATAATTACAAGCCCTTCAGCATTTCAAAAAGTAAAACAAGCATTAGATGAAACATTCAAAATTGAAAATTATACAACAACTGAAGTTACATATATCCCAAATACCGAAATTGAAGTAGATGAAGAAAAAGCAAGCCAAATTGAACAATTTGTTGACTTTTTAGAAGACGATGAAGACGTCCAAGAAGTTTGA", "aa": "MFQKFSKEIIVAATLGGPDPDSNPSLKLAIAKAKAKSMPKANIEKAIAKVAGGSKEAANYQSYLYSGTAFGGVNFIVSCLTDNFNRLSSNIKHFFSKHNGQLGKQGIIPYVFDQKGLIEFPSNVSGEEETMMTVLDAGAEDFRNEDGTYTIITSPSAFQKVKQALDETFKIENYTTTEVTYIPNTEIEVDEEKASQIEQFVDFLEDDEDVQEV", "aa_hexdigest": "3c85c995cde31dbc5659588f2b5a0ada", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_D1J7Z0", "query_cov": 1.0, "subject_cov": 0.8765432098765432, "identity": 0.995, "score": 398.0, "evalue": 2.28e-139, "valid": true, "gene": "TACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q98PU5", "cog_id": "COG0217", "cog_category": "KJ" }, "pscc": { "uniref50_id": "UniRef50_Q98PU5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PU5" ], "product": "Probable transcriptional regulatory protein MYPU_6240" }, "genes": [ "tACO1" ], "id": "LMJBGEANGM_499", "locus": "GCA013368015_00476" }, { "type": "cds", "sequence": "contig_1", "start": 267381, "stop": 268343, "strand": "+", "frame": 3, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_175936698.1", "SO:0001217", "UniParc:UPI00158CBBE6", "UniRef:UniRef100_UPI00158CBBE6", "UniRef:UniRef50_A0A454C9E8", "UniRef:UniRef90_A0A454C9E8" ], "nt": "ATGTATTTAATAAGAGGAGAAGATAGTTATTTAATAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGATGATTATTCAGTAATTACTTACAATGAATCTATTGATTTAGAACAACTATCAGATGATATATTTAACCAATCATTATTTTCTTTTAAAAAACAAGTCATAGTTTTAAAAAACATTGATTTTTTTAATTCTAAGAAAAAAAATGATAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGTCAAGATAACAATTTAATATTTATTTTGCAGGATATTAAAAAAAATGATATTTCTTTTAATCCTTCATTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAATATATCTTCAACTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATCATTAATGAAGTAGATAAATTGCTATTGCAAAATAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAACAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAGATTTATTGCACGTATTTGTTGATGGAAAATACTAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAGAACAAACAAGCATAAATTAAAAGAATTAATAGGGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAA", "aa": "MYLIRGEDSYLINQELQKIIDLKFENQDDYSVITYNESIDLEQLSDDIFNQSLFSFKKQVIVLKNIDFFNSKKKNDNKKMNDFLQLLEKGQDNNLIFILQDIKKNDISFNPSLAYKKIEYISKIIEIKKPSDKDVYNYIKEYILKNGGNISSTTLLDFLSMFPNDLQQIINEVDKLLLQNKNIDMLLINNSNIISSDNIEFNLSNCILKQKSIDIIEAVDEQKKYGNGASEILSQISYLFYQIYCTYLLMENTSDNEYISKTLSIHSYRIKLFMSYIKRTNKHKLKELIGLLADIDIAIKSGLLDEENALNKFILFYIEI", "aa_hexdigest": "1f961418f4367f2d88430773fdfa278d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158CBBE6", "ncbi_nrp_id": "WP_175936698.1", "uniref100_id": "UniRef100_UPI00158CBBE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CBBE6", "RefSeq:WP_175936698.1", "UniRef:UniRef100_UPI00158CBBE6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CBBE6", "uniref90_id": "UniRef90_A0A454C9E8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9E8", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A454C9E8", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9E8" ], "product": "DNA polymerase III subunit delta" }, "genes": [ "holA" ], "id": "LMJBGEANGM_500", "locus": "GCA013368015_00477" }, { "type": "cds", "sequence": "contig_1", "start": 268354, "stop": 268539, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCATTGCAATTGATGATATATTATTGAAATGCTATGAAATCAATAGAAATTATGAATTTAATGATTGA", "aa": "MKKISIYELNLSTFNNKKGLNKYLSIIEKIEYLKSLNIDIIAIDDILLKCYEINRNYEFND", "aa_hexdigest": "992aac5f3fde38e0dba9e482c0ac1027", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.408999999998, "isoelectric_point": 6.194790840148925 }, "id": "LMJBGEANGM_501", "locus": "GCA013368015_00478" }, { "type": "cds", "sequence": "contig_1", "start": 268546, "stop": 268950, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTTAAAAAATTAGTTGAACTTTGCTTGGCAAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAGCATTTAATTTCTATTTATCGAAACAAAAGCAGTGAAAATGAATCGAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTCTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTCTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGCATTAAGGGATTTATTCTTGAAGATTTTGAATTTTTAATTGACAAAAAAACAAATAACATAAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTAGTTCTATGGTTATTTGA", "aa": "MQDFKKLVELCLANNIKIMPTINIAKLKKSIIAKKHLISIYRNKSSENESNKNDIESYLLTQEFLVPTIENISNLSVFLKEIISFYSVLGIKGFILEDFEFLIDKKTNNIKSKFSFLLDIYRIIKHINFSSMVI", "aa_hexdigest": "9b26a3811d7bbc46c180ca36c1813662", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15595.25939999999, "isoelectric_point": 9.277983665466309 }, "id": "LMJBGEANGM_502", "locus": "GCA013368015_00479" }, { "type": "cds", "sequence": "contig_1", "start": 269242, "stop": 269727, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAAGAATATTTTTAATTTCAATAACATTAATTTTAATGAAGAAAAAAGATTTTATCAATCCAAAAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCGTGGACTAATAACATTCAAAATTTAGATTTTATACAGCCAAAATTGCGTAACAAAATTAATGTTGAACAACAGTTAACCAGTAAAGATAGCACATTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCTAAATATTCAATTTATTTTAAGGGTTTTCCCAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGTGAAAATAGAAAACTTTTGATTTTATTAAACTTGAATTCATTTCCAATAAAATCATACATATGA", "aa": "MFLMLAKNSYSLYNGDELGILGTDSKNIFNFNNINFNEEKRFYQSKNISSQKYIESQIKFNPITLQIEFPWTNNIQNLDFIQPKLRNKINVEQQLTSKDSTFNFYSNLVRILNKSKYSIYFKGFPKVLLCNNLISIEYSTSENRKLLILLNLNSFPIKSYI", "aa_hexdigest": "210fd41c040bcc99891300004fb788ff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18997.581400000006, "isoelectric_point": 9.44772891998291 }, "id": "LMJBGEANGM_503", "locus": "GCA013368015_00480" }, { "type": "cds", "sequence": "contig_1", "start": 269887, "stop": 270009, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCAGCCGTCTTAGCTTTTTTAATTTGA", "aa": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLI", "aa_hexdigest": "91c0d7081103885315333806e60440c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4222.982499999998, "isoelectric_point": 5.8228364944458 }, "id": "LMJBGEANGM_504", "locus": "GCA013368015_00481" }, { "type": "cds", "sequence": "contig_1", "start": 270178, "stop": 270324, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACATTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGA", "aa": "MGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNL", "aa_hexdigest": "f443fe4d2404f73fc65af4bcedb9eea7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5711.5286, "isoelectric_point": 8.889174842834475 }, "id": "LMJBGEANGM_505", "locus": "GCA013368015_00482" }, { "type": "cds", "sequence": "contig_1", "start": 270533, "stop": 270904, "strand": "+", "frame": 2, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "EC:2.7.8.5", "GO:0008444", "GO:0008654", "GO:0016020", "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F", "UniRef:UniRef90_UPI001E3AA40F" ], "nt": "ATGATTCCTTTTATTGTTTTTCTTTCAACTTCATTTGGTTTAATTTTAAATGGTGTTCAACTTTCTTACAGAACCAACAAAGGAATAGTATTATCCATTTTATATTGGATAAATGTTGCAATTTTTATCATTGCAATGATAACTGATTTTTTAGATGGATTTTATGCAAGGAAAACAAAGACCGTATCTTCATTTGGAAAAATATTTGATCCAATTGCAGATAAGGTTGCAACTACATTGATGTTATTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCCGTAAAGAAAAACATTGAAGTATCAGCAAATTGA", "aa": "MIPFIVFLSTSFGLILNGVQLSYRTNKGIVLSILYWINVAIFIIAMITDFLDGFYARKTKTVSSFGKIFDPIADKVATTLMLLFLATYNFVYIPIAILFIIRDIIVDGTRVYAVKKNIEVSAN", "aa_hexdigest": "47fc7cedcc0ae0571d8731e1ec6496a9", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgsA" ], "truncated": "both", "pseudogene": { "start": 270446, "stop": 270980, "observations": { "insertion": [ 270962 ], "mutation": [ 270506, 270902 ], "stop": [ 270506, 270902 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3AA40F", "query_cov": 1.0, "subject_cov": 0.6949152542372882, "identity": 0.9670000000000001, "score": 224.0, "evalue": 2.19e-73, "gene_start": 1, "gene_end": 123, "reference_start": 30, "reference_end": 152, "reference_sequence": "MKNKSQKKQKIIVSKFGVANWLTVLRMLLMIPFIVFLSTSFGLILNGVQLSYKTNKGIVLSILYWINVAIFIIAMITDFLDGFYARKTKTVSSFGKIFDPIADKVATTLMLLFLATYNFVYIPIVILFIIRDIIVDGTRVYAIKKNIEVSTNWFGKIKTILVSLALVVISIGFLLYR" }, "paralog": false, "identity": 0.9548022598870056, "score": 316.0, "evalue": 7.95e-112, "description": "Internal stop codon at 270506, 270902. Frameshift due to insertion around 270962. Nonsense mutation around 270506, 270902.", "psc": { "uniref90_id": "UniRef90_UPI001E3AA40F", "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_UPI001E3AA40F", "go_ids": [ "GO:0008444", "GO:0008654", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3AA40F", "EC:2.7.8.5", "UniRef:UniRef50_UPI001E3AA40F", "GO:0008444", "GO:0008654", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3AA40F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" } }, "id": "LMJBGEANGM_506", "locus": "GCA013368015_00483" }, { "type": "cds", "sequence": "contig_1", "start": 271369, "stop": 271713, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3", "UniRef:UniRef90_UPI000B181AC3" ], "nt": "ATGAAATCAAAGAAAATAAACTTATTAAAATTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAAAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCGTTTGTTAATGATGGCAATGTTCCATATGTCAATTTGCTACAAATGATAAATTCCTTAGATGGATTTTTTGATAGAAACTATATAAATTTTTCGACAAATATTCTATTTTCTGAATTAAAAATGACAACTAAATTTGGATATATTTCGTTTAACTGA", "aa": "MKSKKINLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFVNDGNVPYVNLLQMINSLDGFFDRNYINFSTNILFSELKMTTKFGYISFN", "aa_hexdigest": "15dfa7022abbecdf7f17f4e25103b6fd", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000B181AC3", "query_cov": 1.0, "subject_cov": 0.8837209302325582, "identity": 0.991, "score": 216.0, "evalue": 9.91e-71, "valid": true, "uniref50_id": "UniRef50_UPI000B181AC3" }, "pscc": { "uniref50_id": "UniRef50_UPI000B181AC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12966.863299999983, "isoelectric_point": 8.886015892028809 }, "id": "LMJBGEANGM_507", "locus": "GCA013368015_00484" }, { "type": "cds", "sequence": "contig_1", "start": 271732, "stop": 272259, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE", "UniRef:UniRef90_UPI0034D962DE" ], "nt": "ATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAAGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATAAAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTACCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATAAAATCTAATGTGCAAGAAATTCCTCAAATTAGAAATGGCAAATTTCAAAACTCACTTATTCCCAATGACATAGTTGAAGCAAAATTTAACTTCACTGCCTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTCTACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCGACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACTTGA", "aa": "MPNTYYEYITKSPRKTNFNKYLKSKSYYSKDFNKNKIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNGKFQNSLIPNDIVEAKFNFTALLIDYFYGLKEYNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHT", "aa_hexdigest": "1de2397cc9c60ad537b9fb7357a23ae6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 271432, "stop": 272319, "observations": { "mutation": [ 271711, 272257 ], "stop": [ 271711, 272257 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D962DE", "query_cov": 1.0, "subject_cov": 0.5537974683544303, "identity": 0.983, "score": 345.0, "evalue": 4.52e-118, "gene_start": 1, "gene_end": 175, "reference_start": 122, "reference_end": 296, "reference_sequence": "MKSKKINLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFANDGNVPYVNLLQMINSLDGFFDKNYINFSTNILFSELKITTKFGYISFNWKTNKITMPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNGKFQNSLIPNDIVEAKFNFTALLIDYFYGLKENNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFY" }, "paralog": false, "identity": 0.9694915254237289, "score": 581.0, "evalue": 1.74e-213, "description": "Internal stop codon at 271711, 272257. Nonsense mutation around 271711, 272257.", "psc": { "uniref90_id": "UniRef90_UPI0034D962DE", "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D962DE", "UniRef:UniRef50_UPI0034D962DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20884.52600000001, "isoelectric_point": 9.17399616241455 }, "id": "LMJBGEANGM_508", "locus": "GCA013368015_00485" }, { "type": "cds", "sequence": "contig_1", "start": 272266, "stop": 272394, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4", "UniRef:UniRef90_UPI001E356EC4" ], "nt": "ATGCCTTCATTTTATAATCACAAGGATTCTAGCATTGCAAAAGATTTCTATGAATATACCACAAATAAAAGAGAAAGAATTTATCAATCTACAATAAAATATTTAAAAAATAATAAATTAAATAATTGA", "aa": "MPSFYNHKDSSIAKDFYEYTTNKRERIYQSTIKYLKNNKLNN", "aa_hexdigest": "ff78031624260bd747f455f2684c0b03", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 272182, "stop": 272376, "observations": { "mutation": [ 272257 ], "stop": [ 272257 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E356EC4", "query_cov": 0.8571428571428571, "subject_cov": 0.5373134328358209, "identity": 0.9440000000000001, "score": 71.2, "evalue": 1.69e-15, "gene_start": 1, "gene_end": 36, "reference_start": 29, "reference_end": 64, "reference_sequence": "MLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFYEYTTNKRERIYQSTRKYLKIIN" }, "paralog": false, "identity": 0.953125, "score": 124.0, "evalue": 2.93e-39, "description": "Internal stop codon at 272257. Nonsense mutation around 272257.", "psc": { "uniref90_id": "UniRef90_UPI001E356EC4", "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E356EC4", "UniRef:UniRef50_UPI001E356EC4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5164.719000000001, "isoelectric_point": 9.573119926452637 }, "id": "LMJBGEANGM_509", "locus": "GCA013368015_00486" }, { "type": "cds", "sequence": "contig_1", "start": 272569, "stop": 272808, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAATATTATTGTGGATTTATCAACTAATAGTGGTGGCAATATAGGTGCCATGCAAAGAGGATTGGGTTTTATTACAGATGAACCAATATTAAACAAAACATATAATCGTTTAGATGAAGAAATAAATATTAGATCTGTTGAAGTTGATACAGATGGCGATGGAGATTACAAAAACGATGCTTATTCTGAATACAATTGA", "aa": "MKLIKKNLRNMKIKNIIVDLSTNSGGNIGAMQRGLGFITDEPILNKTYNRLDEEINIRSVEVDTDGDGDYKNDAYSEYN", "aa_hexdigest": "1efe6fbcb76a50a6008156400ba2ef52", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.955099999994, "isoelectric_point": 4.98173313140869 }, "id": "LMJBGEANGM_510", "locus": "GCA013368015_00487" }, { "type": "cds", "sequence": "contig_1", "start": 272872, "stop": 273102, "strand": "+", "frame": 1, "gene": null, "product": "S41 family peptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3", "UniRef:UniRef90_UPI0005133DA3" ], "nt": "ATGAAATTAGGCAAGATAATAGGACAAAAAACAGGCGGTGGTGCAGCATCAATTTTACCTGTTGTGTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATAATTTATCTGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGCGGAATAGAACCCGATATTTATTTAGAATACAAAGATTTTTATAATGACCAAAAACTATTAGAAATAATAAAAAATAAATAA", "aa": "MKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNNLSVTMKNNTIKSIEGGIEPDIYLEYKDFYNDQKLLEIIKNK", "aa_hexdigest": "acdb24b4ee0f58b34eb6fcedffcaac12", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 272770, "stop": 273102, "observations": { "mutation": [ 272806 ], "stop": [ 272806 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005133DA3", "query_cov": 1.0, "subject_cov": 0.628099173553719, "identity": 0.987, "score": 147.0, "evalue": 1.53e-44, "gene_start": 1, "gene_end": 76, "reference_start": 46, "reference_end": 121, "reference_sequence": "MGLTPHFSTLPGDYKNDAYSEYNWYLLTGVNTFSAANTFTSIFKEMKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNNLSVTMKNNTIKSIEGGIKPDIYLEYKDFYNDQKLLEIIKNK" }, "paralog": false, "identity": 0.9818181818181818, "score": 215.0, "evalue": 1.35e-73, "description": "Internal stop codon at 272806. Nonsense mutation around 272806.", "psc": { "uniref90_id": "UniRef90_UPI0005133DA3", "product": "S41 family peptidase", "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005133DA3", "UniRef:UniRef50_UPI0005133DA3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3" ], "product": "S41 family peptidase" } }, "id": "LMJBGEANGM_511", "locus": "GCA013368015_00488" }, { "type": "cds", "sequence": "contig_1", "start": 273586, "stop": 274125, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD2D7", "UniRef:UniRef90_UPI0034A44355" ], "nt": "ATGTTTAAAGAATTTAAAAATGGAACTAATGTTTTCATTGAATATTTAACTCCAATATTGAATAGCTTTAAAGAAAAACATGAATTTTTAAAAACAAATGAAAGCAAATATAATGAATTAAAAAACATGTTAATAAAACATTTAGAATATATTTTAGATTACTTTTGCAATCTTAAATTTGATTATTTATTCGAAGGTAAAGATCCAAAACAAACACAATATGATAATAAAGGTGCAGGAAAGAAGGTATTGCTAATTTGGGGTGGATGGCCCATATTATTTGATGTAGAAAAATTTATAGAGCCAAAATATTCTGATCCATCCGGCAATGATTTAAAAAAGAAATTAAATGATATGGTAAATTCAAATAAAATAACAAAACAAGAATATGAAATTATTTTTAATGAATTTAAGGGAATAATATTGATATTTAAGCAACTAATCACAAATATTTATAGTAAAGATTTAAAAAATGTTTTAATGTCTAAACTGCCAAAAGTTTTTGGAGCAATAAATGATGCTAAAAAAAGTGCTAAATAA", "aa": "MFKEFKNGTNVFIEYLTPILNSFKEKHEFLKTNESKYNELKNMLIKHLEYILDYFCNLKFDYLFEGKDPKQTQYDNKGAGKKVLLIWGGWPILFDVEKFIEPKYSDPSGNDLKKKLNDMVNSNKITKQEYEIIFNEFKGIILIFKQLITNIYSKDLKNVLMSKLPKVFGAINDAKKSAK", "aa_hexdigest": "4de3163ca9d32bd447d703c3b9b0ce59", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 273586, "stop": 274425, "observations": { "mutation": [ 274137, 274164 ], "stop": [ 274137, 274164 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A44355", "query_cov": 1.0, "subject_cov": 0.4323671497584541, "identity": 1.0, "score": 363.0, "evalue": 7.07e-124, "gene_start": 1, "gene_end": 179, "reference_start": 236, "reference_end": 414, "reference_sequence": "MEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKNSNEFKKYQENVKLNIETNIEKKINEARKKQQKQAAHQNVIKAIIEQYKKFNEEIEKTLFELANKAINVFGQFNSNKESIKKLYNILINLIINTNDEMLNVLLELGINDKVSTGLRELTADWVLSKTVGAWAIKMFKEFKNGTNVFIEYLTPILNSFKEKHEFLKTNESKYNELKNMLIKHLEYILDYFCNLKFDYLFEGKDPKQTQYDNKGAGKKVLLIWGGWPILFDVEKFIEPKYSDPSGNDLKKKLNDMVNSNKITKQEYEIIFNEFKGIILIFKQLITNIYSKDLKNVLMSKLPKVFGAINDAKKSAK" }, "paralog": false, "identity": 0.989247311827957, "score": 546.0, "evalue": 2.56e-198, "description": "Internal stop codon at 274137, 274164. Nonsense mutation around 274137, 274164.", "psc": { "uniref90_id": "UniRef90_UPI0034A44355", "uniref50_id": "UniRef50_UPI00193AD2D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A44355", "UniRef:UniRef50_UPI00193AD2D7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AD2D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD2D7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 21073.32430000001, "isoelectric_point": 9.205263328552245 }, "id": "LMJBGEANGM_512", "locus": "GCA013368015_00489" }, { "type": "cds", "sequence": "contig_1", "start": 274162, "stop": 275805, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34", "UniRef:UniRef90_UPI0005CB0F1B" ], "nt": "TTGGATACATTAGTTGAACAAGAAAACATTACATCTTTAAAAAATAATGAATATTTAACATCTAAAAAATCTTCAATGATTTCAAAAAATGACTTTGAATTAAACAAGCAAAAAAGTAATGAACTAGCAGGGTTGGAATTAAATATTTCAGAAATCAATGCCAATGCCAATGGAAAATTAGAAATAGCCTTTTCGTTATTGAATAAAACATTTAAACATGGAACAATAGAATCAAACAAAAAGACAGTTACATTCAATTTCTTAAGTGATGAAAATTACACATTTAATTTAATTAATAACTTAAGCATTTCAAAAGAATCAACTTATGTAACTGATGGTGTTTATACTAAAGCAGATTTTAAAAATAAAATTGCTAACAATAAACAAGAAATTGCTAAGTTATTAGGTATTTCTGATTTTCTAAATCAAAACTCAATTATTGAATTGAATCAAGAAAATGAAAACGCTATTGTGTCATTAAATAAATTTGTAAATACTACTACTGTAAAATATGAATTTATTTTAAAGATAAAATCAGCAAATGATAAACTTTTTAAAAAATTCTCGGGTGAAATTTCTATTGCTAATATTATGAATAAACGAAATTTAAAAAATTTTGCAAATAACTTTGATTTTGATTTTAAAGATTACACCAAAAAAGAATTAGCAAAATCAATGACAATAGATGAATCAATAGAAAAAGGCTACAATTTCTTTTTTAGAGAAAAAATTAATTTTGGTAGCGGAGAAGAATCTTATATTATTGATGATTTTAAAATTCAAAAAGATTATAAACTACTGGCAAGTGAAAATGCAAATGATATAAATATACAAATAACATTCAAATTAGACGATTATAGTGAAATAAAAACCTTTACTAAATCAGGTTTCAATGACAAAGCAAGCATTGAAAAAATAAAAGAAGAATTAAAAAATGAACCAAAAAGGCTTGACGATTTATTAGATAAACTTACAATGGAATTCACTAAAAATAATGATTTTGAAAAGCAATCTATTGATGTTAAAGATATTACAATTAATGATTATAAATTTAATCTAAAAGATAGTGATTTTGCAGATATCTATGAAATAGAGGCAGTTGAATATGATAAAACATTAGGAAAATTAACTTATAAAATTAAGTTAAAAACGAAGAAAAGTTTATTGAAAGAATATAGCAAAAATCCAATAGAGTCTACTAAATCAAGAACATTTGAATTTTATGGATTTGCTAAGGATATAAGTGAAATAACTAAAAATTTAGAATCGGATGTAAAAAATTCAAATGAATTTAAAAAATATCAAGAAAATGTTAAATTAAATATTGAAACAAATATTGAAAAAAAGATTAATGAAGCAAGAAAAAAACAACAAAAACAAGCCGCTCATCAAAACGTAATTAAAGCAATAATTGAACAATATAAAAAGTTTAACGAAGAAATAGAAAAAACGTTATTTGAACTAGCAAATAAAGCAATTAATGTATTTGGTCAATTTAATTCCGACAAAGAATCTATTAAAAAACTTTACAACATCTTAATTAATTTAATTATTAACACCAATGATGAAATGTTAAATGTATTATTAGAATTAGGCATTAATGATAAAGTTTCTACTGGTTTAAGAGAGTTAACAGCAGACTGA", "aa": "MDTLVEQENITSLKNNEYLTSKKSSMISKNDFELNKQKSNELAGLELNISEINANANGKLEIAFSLLNKTFKHGTIESNKKTVTFNFLSDENYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENENAIVSLNKFVNTTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLKNFANNFDFDFKDYTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDFKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNEPKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFYGFAKDISEITKNLESDVKNSNEFKKYQENVKLNIETNIEKKINEARKKQQKQAAHQNVIKAIIEQYKKFNEEIEKTLFELANKAINVFGQFNSDKESIKKLYNILINLIINTNDEMLNVLLELGINDKVSTGLRELTAD", "aa_hexdigest": "dbbb5a92bf38e037a76880b6e694a9ea", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 274407, "stop": 276105, "observations": { "insertion": [ 274443 ], "mutation": [ 275928 ], "stop": [ 275928 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "query_cov": 0.8007312614259597, "subject_cov": 0.49158249158249157, "identity": 0.9790000000000001, "score": 793.0, "evalue": 6.75e-280, "gene_start": 22, "gene_end": 459, "reference_start": 448, "reference_end": 885, "reference_sequence": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLALEKHNKKPSEIKNVDFNKKNIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTIKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNHFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKFIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDKGNLKIKFVVKTKEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEGKEIHLNISDSKYTIEGNPSIIADDDQGILSVSWTIKVKEEKFKTFIVSESRISKKITNFLTKKQNEINIEQKRLDTLVEQENITSLKNNEYLTSKKSSMISKNDFELNKQKSNELAGLELNISEINANANGKLEIAFSLLNKTFKHGTIESNKKTVTFNFLSDENYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENENAIVSLNKFVNTTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLKNFANNFDFDFKDYTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDFKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNESKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKKSDEFKKYLENVKLNIETNIEKKINEAKKNNKKKPLIKT" }, "paralog": false, "identity": 0.9557522123893806, "score": 1035.0, "evalue": 0.0, "description": "Internal stop codon at 275928. Frameshift due to insertion around 274443. Nonsense mutation around 275928.", "psc": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005CB0F1B", "UniRef:UniRef50_UPI0034A1CB34" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "LMJBGEANGM_513", "locus": "GCA013368015_00490" }, { "type": "cds", "sequence": "contig_1", "start": 275926, "stop": 277083, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34", "UniRef:UniRef90_UPI0005CB0F1B" ], "nt": "GTGGGATTTAATACATTACTTAACATAGAAAGACAAGAAACATTAAAGAAACTTTCTAATTTAGTTAATTCAATTGATCTGAACGAATTTTTAGCGACTGAAAAGCACAGCAAAAAACCTTCGGAAATCAAAAATGTTGATTTCAATAAAAAAAATATTGATGGAGCAATGTTAGTTATTGAAAAAATGCAAGCCAATGACGAAGAAGGAACATTGTTAATTAGTTTTTATTTAAAAAGTACTAATCCAAAATTCAGCGATTTAAAAACGGAAATCAAAACAGTAAAATTGATAGATTTTTTAACAAATGCACAATTTATTAATAACCAAAGAAATGAAACAATTGCTTATTTAAATGAATTAATAGGAAATTTAAATATAAAAGTAAGCGATAAGAAACCTGAAACTGCTATTAAAAGATTTGCATCTTCAATCAAACAATCAGATTTAAATATTGAAATTCCAAAGCCAAATAATAATTTTAAAAATATTGAAGTTGTTGTAAAGCAATTAATTCCAAACGATGAAAGTGGAAAATTAAGTATTAGATTAGCAATCAAGCAATTGAACAAACAAATTAATGAATATTGCGAATCAAGCAAACTTATTGAAGTTGAATTAAGTGGTTATAAAACTAAAACAATGGATAAAAATGAAAAAATAGAATCTTTAAATAACGTTGCCAAAGAAACAACAACATTAAGATTAATTTCTTCAAAAGATATTGCCAAAACATTTGCTTCTGATGTTCAAAAAAACGATTTTGAAACAGAAATTAGCAATGATTTTTATATTGAAATTTTAGAAATAAGTGAATCAAATGATGATAAAGGAAGCTTAAAAATTAAATTTGTTGTTAAAACAAAAGAAAAGCAATATAAAGATTTAAAATCAAATGAACAAACTATAACATTGTCAGGTTTTTATACAAAAGAACAATACTTAAAGGCTTGCAAGCAAGAGTTGAATAAATTAATTAGCGATGATAAGATAGAAGCAAATTTAGTTTTTGATAAAGAGAATGATAAGTCAAATATATTACCTTCAAATGTTGAAGGTAAAGAAATTCATCTAAATATAAGCGATTCAAAATATACTATTGAAGGAAATCCATCAATTATTGCAGATGATGATCAAGGAATTTTGTCAGTAAGCTGA", "aa": "MGFNTLLNIERQETLKKLSNLVNSIDLNEFLATEKHSKKPSEIKNVDFNKKNIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTVKLIDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNNFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKLIEVELSGYKTKTMDKNEKIESLNNVAKETTTLRLISSKDIAKTFASDVQKNDFETEISNDFYIEILEISESNDDKGSLKIKFVVKTKEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEGKEIHLNISDSKYTIEGNPSIIADDDQGILSVS", "aa_hexdigest": "8e56ae32bae138d879b307e534e9048e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 275623, "stop": 277083, "observations": { "mutation": [ 275928 ], "stop": [ 275928 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "query_cov": 1.0, "subject_cov": 0.43209876543209874, "identity": 0.9690000000000001, "score": 693.0, "evalue": 3.96e-243, "gene_start": 1, "gene_end": 385, "reference_start": 1, "reference_end": 385, "reference_sequence": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLALEKHNKKPSEIKNVDFNKKNIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTIKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNHFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKFIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDKGNLKIKFVVKTKEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEGKEIHLNISDSKYTIEGNPSIIADDDQGILSVSWTIKVKEEKFKTFIVSESRISKKITNFLTKKQNEINIEQKRLDTLVEQENITSLKNNEYLTSKKSSMISKNDFELNKQKSNELAGLELNISEINANANGKLEIAFSLLNKTFKHGTIESNKKTVTFNFLSDENYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENENAIVSLNKFVNTTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLKNFANNFDFDFKDYTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDFKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNESKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKKSDEFKKYLENVKLNIETNIEKKINEAKKNNKKKPLIKT" }, "paralog": false, "identity": 0.9362139917695473, "score": 868.0, "evalue": 1.57e-314, "description": "Internal stop codon at 275928. Nonsense mutation around 275928.", "psc": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005CB0F1B", "UniRef:UniRef50_UPI0034A1CB34" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "LMJBGEANGM_514", "locus": "GCA013368015_00491" }, { "type": "cds", "sequence": "contig_1", "start": 277522, "stop": 278301, "strand": "-", "frame": 3, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC", "UniRef:UniRef90_UPI00193A2EAC" ], "nt": "ATGAAAAAGAATACAAAAATCAAAAGCATTTCCATTTTAAGTGCTACTGCCATTTTACTTGTATCAGGAGTCATTTCATCAGCCTGCAACAAGACGCAAGATAAAGAAACTGAAACAACAAAATTAAAAGAACAAACAAGAAATATTAAACCAAATTTAAAACTTTCTATTAAACAGGATAAGATACTTCCAAGCGAAATTAATTCATCAAATTTTTATGTCGATAATCTTGATAAAGAAATATCATTTGAAATCTCTAATATTATTGCAGATGATGTAAACGGAAATGTTATTATAAATTATTATTTTACAAAATCAATAATCGATTCTAAAACAAAACAACCACTGTTAATAAAATCAAATATGTTTTCTCAAAAATATACAGGATTTAAAGCGTTGGTTGCTCCTTTAAATTCGTCAATTGAACAAGTTAATCTAGAAATAAATGAATTTAATAAAAAAGACTTTTTGCCTTCGCAATATATTAAATTGCAAAGCAATTTTTCATTTAAAAACATCGATTCCCAATATGAATTAAAAACTGAATATATCGCAAACGATGAACAAGGCAAAATAGAAGTTAAATATTATTATGTACAAAAAGACAAAGATATAAAATCAAAAATTATTTCAAAAACATTTTATGGTTTTAATTCATTGAATAATATAGCTAATATGCAAAAAGAAGCTAACACTTTAAATAAATTAGGAAATGAGATATCTTTAAAATTAAAGAAACAAATTAATAAATCAGAAACATATGCTTCAACATTGAATTGA", "aa": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDKILPSEINSSNFYVDNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTKQPLLIKSNMFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDEQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANMQKEANTLNKLGNEISLKLKKQINKSETYASTLN", "aa_hexdigest": "a72ba5a954844d0155c62b372f528794", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 277219, "stop": 278301, "observations": { "mutation": [ 277377, 277524 ], "stop": [ 277377, 277524 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A2EAC", "query_cov": 1.0, "subject_cov": 0.6675257731958762, "identity": 0.981, "score": 466.0, "evalue": 2.8e-163, "gene_start": 1, "gene_end": 259, "reference_start": 1, "reference_end": 259, "reference_sequence": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDKILPSEINLSNFYVNNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNTFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDGQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANMQKEANTLNKLGNEISLKLKKQINKSETYASTLNWSDFETSKKLNSAYLRSNGELIFDDINGEVKLNYYFEKQYIDKKTGASYWIKSKVYTTKITGFKSFLKNLNIAVDKVNLSINEKNINTILPSQYIQNLSNFKFDNLNNEYELQKKFSYDDQKGELTISY" }, "paralog": false, "identity": 0.975, "score": 678.0, "evalue": 2e-249, "description": "Internal stop codon at 277377, 277524. Nonsense mutation around 277377, 277524.", "psc": { "uniref90_id": "UniRef90_UPI00193A2EAC", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A2EAC", "UniRef:UniRef50_UPI00193A2EAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "LMJBGEANGM_515", "locus": "GCA013368015_00492" }, { "type": "cds", "sequence": "contig_1", "start": 278384, "stop": 279388, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175936704.1", "SO:0001217", "UniParc:UPI00158B45E9", "UniRef:UniRef100_UPI00158B45E9", "UniRef:UniRef50_D1J7Z7", "UniRef:UniRef90_D1J7Z7" ], "nt": "ATGAAAAAATCCAAAAAAATTTTATTAACTATGGGGGTAGCACTTCCATTATTAGCCTCAACTCCATTGGTTGCTGCAGGTTGCGTAAAAACTAAACCAGAAGTTAAACCAGAAGATCCAAAGAATCCTGAAGGTGAAAAACCAGGAACAACACCCGAAAAGAAGCCTGAAGGTGAAAAACCAGGAACAACACCAGAAGTTAAACCAGAAGAAAAAAGCGAACTTCAAAAAGCAACTGAAGGTATTGCGGATGCTACAGTTAAAGCAAATTTTGAAAGTGAATTAAAGAAATATCGTGAAGAATTAAAAACTAAATCAGAAGCCATTTTAAAGCCAATTATTGAAAATAAAGAAATTAAAGCTGCAGTTTCTGAAAAAGAATTGCCAAATATTACAAAAACATATAATGAAGCAATTTCTAAATTAAATGAAATTTTAGATGATATTATTTTGTCAACATTAAAAGAATACAACACAAATAAAACAAAAGTTTCTGATGGATTAATGACAATTAAAAATTATTTAACAAAAGAAAAAATTTCTGATTTAATTAAAAAAGAAAACGGGTTGGCAGATTTCTTGGTTGCATTTGGAAAAAATATTTCTAAATATGCAAAATCAATTTTTGAAACACTAAAAACAAACGAATCAACAAAAGCAATAGCTCAATTGAATGACTTAATTACAGCTGTTGACAATAAATTTGCTGGAAAAGATAATTTATTTGAAACAACCGCTAAGAAATTAAAAGAATTTGATTTTAGCAAATTGGCTGAAGAAGGATATGTAAAAACATTTGAAAAATTAATTACATTATTATCTGACGCTAAGGTAGAAGCAGTTGCGGTATTAAAATCCGTAAAAATGTCTGATGAAGATATTGAAAAAGTAAAAGGCGTAATTAGACCAATTTACAACGTTTTAAATGAAGGTCTAAAAAAATCAATTAACTTATTTGAACAATCATTGAGAGATTTACCAAAAGTGTTAGAAATCTTAAAATAA", "aa": "MKKSKKILLTMGVALPLLASTPLVAAGCVKTKPEVKPEDPKNPEGEKPGTTPEKKPEGEKPGTTPEVKPEEKSELQKATEGIADATVKANFESELKKYREELKTKSEAILKPIIENKEIKAAVSEKELPNITKTYNEAISKLNEILDDIILSTLKEYNTNKTKVSDGLMTIKNYLTKEKISDLIKKENGLADFLVAFGKNISKYAKSIFETLKTNESTKAIAQLNDLITAVDNKFAGKDNLFETTAKKLKEFDFSKLAEEGYVKTFEKLITLLSDAKVEAVAVLKSVKMSDEDIEKVKGVIRPIYNVLNEGLKKSINLFEQSLRDLPKVLEILK", "aa_hexdigest": "2d036819f7f862d23c21ea23e88593b1", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158B45E9", "ncbi_nrp_id": "WP_175936704.1", "uniref100_id": "UniRef100_UPI00158B45E9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B45E9", "RefSeq:WP_175936704.1", "UniRef:UniRef100_UPI00158B45E9" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B45E9", "uniref90_id": "UniRef90_D1J7Z7" }, "psc": { "uniref90_id": "UniRef90_D1J7Z7", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7Z7" }, "pscc": { "uniref50_id": "UniRef50_D1J7Z7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z7" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_516", "locus": "GCA013368015_00493" }, { "type": "cds", "sequence": "contig_1", "start": 279746, "stop": 279898, "strand": "+", "frame": 2, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855488.1", "SO:0001217", "UniParc:UPI0001BC21A4", "UniRef:UniRef100_D1J7Z8", "UniRef:UniRef50_P35871", "UniRef:UniRef90_B3PMG6" ], "nt": "ATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAA", "aa": "MPREGLTLRCEKCKMENYITKKNKKLHPDKMEVQKYCSKCNAHTTHKEKK", "aa_hexdigest": "a94740e22d2064ea07f0b499858aa7c7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC21A4", "ncbi_nrp_id": "WP_012855488.1", "uniref100_id": "UniRef100_D1J7Z8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21A4", "RefSeq:WP_012855488.1", "UniRef:UniRef100_D1J7Z8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Z8", "uniref90_id": "UniRef90_B3PMG6" }, "psc": { "uniref90_id": "UniRef90_B3PMG6", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "LMJBGEANGM_517", "locus": "GCA013368015_00494" }, { "type": "cds", "sequence": "contig_1", "start": 280179, "stop": 280589, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "TTGGCTAAAAAGAAATACGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTCGAAACATTTGAAAACTTAAAGTCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGCAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGCACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGA", "aa": "MAKKKYATVGVEKDYLILETFENLKSMLPKSKFVLVNGQEFRILKDEEEYSNVQEACNISLQSFEQIKTVLTEGITELEVSNKLGYLMRLFGAEKESFETIVAFGTNAAEPHHHPTDRKLADGDIVKIDFGAQYQG", "aa_hexdigest": "01e59e4a40b1bf253a9a3c195050a784", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 87, "aa_cov": 0.625, "hmm_cov": 1.0, "evalue": 2.30888711910087e-22, "score": 75.3111572265625, "start": 52, "stop": 136 } ], "seq_stats": { "molecular_weight": 15395.32599999998, "isoelectric_point": 5.111382865905762 }, "id": "LMJBGEANGM_518", "locus": "GCA013368015_00495" }, { "type": "cds", "sequence": "contig_1", "start": 280866, "stop": 280991, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "ATGATTATTACTGTTGAACCTGGTATATATATCGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAAAGTATTAAGCAGACCCGAAGATTATGAAAAATAA", "aa": "MIITVEPGIYIENEGGARIEDTILVTKDGSKVLSRPEDYEK", "aa_hexdigest": "4b564575859134acf0642200976ffa80", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 28, "aa_cov": 0.6585365853658537, "hmm_cov": 1.0, "evalue": 1.1633790580827183e-09, "score": 33.81732940673828, "start": 1, "stop": 27 } ], "seq_stats": { "molecular_weight": 4580.1284000000005, "isoelectric_point": 4.442785835266113 }, "id": "LMJBGEANGM_519", "locus": "GCA013368015_00496" }, { "type": "cds", "sequence": "contig_1", "start": 281106, "stop": 281303, "strand": "+", "frame": 3, "gene": "dprA", "product": "DNA-protecting protein DprA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E573C53", "UniRef:UniRef90_UPI001E573C53" ], "nt": "ATGAACAAAGAAGAATATTTAGCTTATTTAAGTTATATAAATAATGGCAAATGGCACCAAATCTATAATGATTTAAAATCACAAATAACATGTCAAAAAACAAAATATGCTGATTTTTGTAGTGACCAAGCAATTAAAAACAAATCATACATAACAATTTTTGATAGTGATTATCCGCTATATTGCAATAATGTTTGA", "aa": "MNKEEYLAYLSYINNGKWHQIYNDLKSQITCQKTKYADFCSDQAIKNKSYITIFDSDYPLYCNNV", "aa_hexdigest": "b339db72e7c68f95f345321a0826057f", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dprA" ], "truncated": "3-prime", "pseudogene": { "start": 281106, "stop": 281526, "observations": { "insertion": [ 281499 ], "mutation": [ 281301, 281457 ], "stop": [ 281301, 281457 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E573C53", "query_cov": 1.0, "subject_cov": 0.4676258992805755, "identity": 1.0, "score": 142.0, "evalue": 1.6e-42, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MNKEEYLAYLSYINNGKWHQIYNDLKSQITCQKTKYADFCSDQAIKNKSYITIFDSDYPLYCNNVWNPPFVLYYYGNKQLLKHKIIMITGNISISNIIEVKDSIKNFGKEYVFCNEAWKGLDIQLIDYLISKIKILLLF" }, "paralog": false, "identity": 0.9712230215827338, "score": 272.0, "evalue": 5.54e-96, "description": "Internal stop codon at 281301, 281457. Frameshift due to insertion around 281499. Nonsense mutation around 281301, 281457.", "psc": { "uniref90_id": "UniRef90_UPI001E573C53", "product": "DNA-protecting protein DprA", "uniref50_id": "UniRef50_UPI001E573C53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E573C53", "UniRef:UniRef50_UPI001E573C53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E573C53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E573C53" ], "product": "DNA-protecting protein DprA" } }, "id": "LMJBGEANGM_520", "locus": "GCA013368015_00497" }, { "type": "cds", "sequence": "contig_1", "start": 281586, "stop": 281864, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTCATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGAGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGCTATAAAAGCACCATGAATAACCTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCAAGAAAAATAACTTCAATAACTGATTTAATATTAAAATAG", "aa": "MLFISEYPSNYHTSKITLKARNRISAAISNYLVLLSCYKSTMNNLIDQFLNIGKEVYCLDDIQNNLNEDNFNKNLINEGARKITSITDLILK", "aa_hexdigest": "a5db2ba9b25349b8803ac4c2f9fbe82a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10577.022299999986, "isoelectric_point": 7.7458700180053714 }, "id": "LMJBGEANGM_521", "locus": "GCA013368015_00498" }, { "type": "cds", "sequence": "contig_1", "start": 281856, "stop": 282101, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGCCTTTAATTAGAGTTATTCATTTAAATGATTCCATGAATGAACTCGGATCAAAAAAAGATCGTCATGCAAACATTGATAAAGGCCATATAGGTTTAGAAACATTGAAAAAATTTGTTCATGATAAAGACTTCGATAATATTCCTATTATTTTAGAAACACCGTTTATTAATAATAAAAGTCCTTACAAAGATGAAATAATAATGCTTTTAGAGAATAATAAAAAAGATAATATTCTATTTTAA", "aa": "MPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLETLKKFVHDKDFDNIPIILETPFINNKSPYKDEIIMLLENNKKDNILF", "aa_hexdigest": "f33896267b82952ef9d8b51e88010bdb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387AC6AF", "query_cov": 0.8641975308641975, "subject_cov": 1.0144927536231885, "identity": 0.7709999999999999, "score": 103.0, "evalue": 9.85e-28, "valid": false, "product": "TIM barrel protein", "uniref50_id": "UniRef50_Q49058" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 68, "aa_cov": 0.8271604938271605, "hmm_cov": 0.9852941176470589, "evalue": 8.15762247439539e-15, "score": 50.677921295166016, "start": 4, "stop": 70 } ], "seq_stats": { "molecular_weight": 9454.862199999992, "isoelectric_point": 6.396228218078614 }, "id": "LMJBGEANGM_522", "locus": "GCA013368015_00499" }, { "type": "cds", "sequence": "contig_1", "start": 282165, "stop": 282707, "strand": "-", "frame": 1, "gene": "nfo", "product": "deoxyribonuclease IV", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_D1J801" ], "nt": "ATGATTAAATTAGGAAGCCATATATCATTTAAAAAACCTGATTATTTAATAGGTTCATTTAATGAAGCTATTAATAATGGCGAGAATTGTTTTATGATTTACTTAGGAGCACCACAAAGTGCTTTTAGAGTTGATAGTTCATTGTATAATATTGAAAAATATTTAAAGCAATGCAATAGTATTATTCCTGTATCTGATATTGTGGTTCATGCCCCATATATAGTAAATTTAGCCAATAGAGAAAAGGCAGATTTTGGGATTAAATTTTTATGTAGTGAAATTAAAAGAATGAATAGTTTTGGCGCTAAATTGCTAGTTTTACACCCTGGTTCATCAGTTGAATATAGCAGAAGCGAAGCATTAGCATCATTAATTCAATCTCTTAAAAAGATAATTGAATCTACTAAGGACGTAATAATATGCCTTGAAATAATGTCAGGTAAAGGCAGTCAAATTTGTAGCAATTTCAATGACTTATTATACGTGATAAAATCCGTTAATTCCGAAAGAATTGCAATATGTTTTGATACTTGCCATGCCTGA", "aa": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKADFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICFDTCHA", "aa_hexdigest": "5580119ba68d62cdb43ed64d89fd637d", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nfo" ], "truncated": "5-prime", "pseudogene": { "start": 281862, "stop": 282707, "observations": { "mutation": [ 282167 ], "stop": [ 282167 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J801", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.9890000000000001, "score": 354.0, "evalue": 3.98e-122, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKVDFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICLDTCHAWDAGYDIKDYENFKSYIKQQNILPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLETLKKFVHDKDFDNIPIILETPFINNKNPYKDEIIMLLEDNKKDNILF" }, "paralog": false, "identity": 0.9822064056939501, "score": 553.0, "evalue": 5.78e-203, "description": "Internal stop codon at 282167. Nonsense mutation around 282167.", "psc": { "uniref90_id": "UniRef90_D1J801", "gene": "Nfo", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J801", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "COG:COG0648", "COG:L", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "LMJBGEANGM_523", "locus": "GCA013368015_00500" }, { "type": "cds", "sequence": "contig_1", "start": 282874, "stop": 283395, "strand": "-", "frame": 3, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "nt": "ATGGTTGAAACAATAAATAAAATATTGAAGATTGAACGTGAATTGCAACAAGAAAATGGATATCCTCCAACAGATGAAGAAATTGCTAAAAAATACGGTGGAGATTTTACTGCTGAAAAAGTAAGATATATTAGAAAAATTAATATCGATCCAATTAGTTTGGATAAAAACATTGGAAAAGAAGAAAATTCTAGTTTTTCAGATTTTGTTAAAGACGAAAGCGTAATTAGTCCAACTTCGTATGCAAGTCATGAAGAGTTAAGTGTTATTATAAATGAAATGATTGAAGAAGGGTTAAGCGATCAAGCTGACCGGGATTTAATTAGAAAACGTTTCGGAATTAACGACTTAAAGGGAAACAAATACAAACCTCATACTTTAGATGAACTTTCAAAAGAATTAGGCATTTCGAAAGAAAAAGTTAGACAATTAGAAACAAAAGTTTTGCGTAAATTGAAGCATCCTCAAAAACGTAGAAAATTGAAGGAATTCTTTAATAGCGATAGCTATGATCTAGACTAA", "aa": "MVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD", "aa_hexdigest": "bb6960937ffe99faca464418640fb8db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0024C90298", "query_cov": 0.9884393063583815, "subject_cov": 0.8142857142857143, "identity": 0.561, "score": 166.0, "evalue": 3.83e-49, "valid": false, "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A448ZYJ5" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "product": "RNA polymerase sigma factor" }, "genes": [], "id": "LMJBGEANGM_524", "locus": "GCA013368015_00501" }, { "type": "cds", "sequence": "contig_1", "start": 283462, "stop": 283722, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04542.20" ], "nt": "TTGCTAACTACAAATGAAGAACAAGCGCTAGCAAAAAAAATGCAAGAAGCTAAAGAAGCAAATAATTATTATAAATATAAAAAAGCTAGAGATATGTTAGTTAAAAGAAATCTCCGCTTAGTAATCAATAATGCAAAGCGTTATAAAAATCGTGGATTGAATTTTATTGATTTGATATCAGAAGGAAATGCCGGAATAATGAAGGCAGTTTCAAAATATGATTACACAAAAGGATTTAAATTTTCAACATATGCTACTTGA", "aa": "MLTTNEEQALAKKMQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYAT", "aa_hexdigest": "09424a3a1800bf47eb30217db6f002da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04542.20", "name": "Sigma70_r2", "length": 54, "aa_cov": 0.627906976744186, "hmm_cov": 1.0, "evalue": 7.123026568558338e-16, "score": 53.37271499633789, "start": 33, "stop": 86 } ], "seq_stats": { "molecular_weight": 10024.499799999994, "isoelectric_point": 9.909451484680176 }, "id": "LMJBGEANGM_525", "locus": "GCA013368015_00502" }, { "type": "cds", "sequence": "contig_1", "start": 283753, "stop": 284490, "strand": "-", "frame": 3, "gene": "rpoD", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "SO:0001217", "UniRef:UniRef50_A0A501X9X8", "UniRef:UniRef90_D1J802" ], "nt": "ATGCAAGATACTAAAAATAATGTAGAATCATCAGTTAAGTGTATAACTAAAATTGTTGCAGACGAGTTAAAAAAAGTCCAAAAGAAAAATAAGGATAAAAAGGCTTTTACTCAAGATGAAATTTTTGAATTCCTAAACAAAAAGAACCTTTACGTTGATGACGAAGAAGCAGAAGCTTTACTTGAAAGCCTTTTTGCAGCTAATCTAGTCTCATCTGATACTGATGACGAAGATATTGAAGATATTGACATAAATTCGTTAGATGATGAAGTTGCTCACGCAACAACTAAAGATGATGATAATGATGACGATGACGCAGAAGAGGAAGAAAAAGATTTTGACCCTTCAAAAATTAAAGAGTTGGAAGAAGAAAATCTTGACGAAGATATAAATATTGATCAAGAATTTGACGATAGTTCAATTAGAAGCAAGGAAGAAGATGACTCGGAAATGTCTTTTGACGAAGATGATAATCATTTTGTTAAAAAGGCATTTAATGATCTTGATGATGAAGAATATAATGATTATTACGGCGAAGATTTTGACGAATATGAAGATGACGATTATCAAACAAACCACAAGCAAGGTAAAAAACAATCATACGATGACTTCGATGCTAACAATTTGCTAAAAGAATACAATGACGAAGATATAAAGTTGAGTGAAGAAAAAGTTTATGATCAAGATGATATAAAATTAACTAATAAGCTAACTGAAACCAACGACATTGTTAAATGA", "aa": "MQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVK", "aa_hexdigest": "915da4e6aba3dd5b9385155b135b4b26", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpoD" ], "truncated": "5-prime", "pseudogene": { "start": 283450, "stop": 284490, "observations": { "mutation": [ 283461, 283464, 283743, 283755 ], "stop": [ 283461, 283464, 283743, 283755 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J802", "query_cov": 1.0163265306122449, "subject_cov": 0.45940959409594095, "identity": 0.94, "score": 372.0, "evalue": 2.62e-124, "gene_start": 1, "gene_end": 245, "reference_start": 1, "reference_end": 249, "reference_sequence": "MQDTKQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVKWYMRWIGKYGKLLTTNEEQALAKKMQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYATWWIRQAITRAVADQARTVRVPVHMVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD" }, "paralog": false, "identity": 0.9571428571428572, "score": 589.0, "evalue": 4.1e-212, "description": "Internal stop codon at 283461, 283464, 283743, 283755. Nonsense mutation around 283461, 283464, 283743, 283755.", "psc": { "uniref90_id": "UniRef90_D1J802", "gene": "RpoD", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A501X9X8", "cog_id": "COG0568", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J802", "UniRef:UniRef50_A0A501X9X8", "COG:COG0568", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9X8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9X8" ], "product": "RNA polymerase sigma factor" } }, "id": "LMJBGEANGM_526", "locus": "GCA013368015_00503" }, { "type": "cds", "sequence": "contig_1", "start": 284550, "stop": 284780, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTTCCAAACGGATGTCAAAATTATAAAATGTTTTTGTGTTTTTTAATAAACAGCATAAAAAATAATAAATCAATAGATGAAACAAATTTTGAATCATATATTACAAACAATATTGATCTTGAAGATAATGCTAAACAAATATATTTAGATTTATTCAAGGAAATTATGAGTCTTAAAAACAAAGAATATACAAAAGAAGAACTAGATAAAAATTTAAATAATTGA", "aa": "MAFPNGCQNYKMFLCFLINSIKNNKSIDETNFESYITNNIDLEDNAKQIYLDLFKEIMSLKNKEYTKEELDKNLNN", "aa_hexdigest": "03418faf7e112560faad31b179b420fa", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9024.135999999991, "isoelectric_point": 4.792686653137207 }, "id": "LMJBGEANGM_527", "locus": "GCA013368015_00504" }, { "type": "cds", "sequence": "contig_1", "start": 285093, "stop": 286352, "strand": "-", "frame": 1, "gene": "dnaG", "product": "DNA primase", "db_xrefs": [ "COG:COG0358", "COG:L", "EC:2.7.7.101", "KEGG:K02316", "SO:0001217", "UniRef:UniRef50_D1J803", "UniRef:UniRef90_D1J803" ], "nt": "ATGGCAGAAGATAATATATTTGAAATTGTTAAACAAAAGTTAAACATTGTTGATGTTATTTCTTTATATATTTCTTTGACAAAAAAAGGAAAGAATTATGTGGCTTGTTGCCCATTTCATGGTGAGAAAACTCCTTCTTTTGTTGTGTCACAAGAAAAACAAATATTTAAATGTTTTGGTTGCTCTAAAAGCGGAAATTTAATAACTTTCATTGAACTTTATACTAAAAAAAGCCCTCTTGAAAGTATCAAGGAGCTTGTTTCAAAATTCAATTTAAATATCGATTTAACAAGGTTTAGTAATAGTATAACTACTACAACAAAAGAACAAGAAGATTTATACAATATAAATCAAATAGCAAATGATATATTTCAATTTGAAATTTTAGAAATTAATCAAAATGAGCAATTAAAATTATTTCTTAAAAAAAGAAGGTTAACAAAACCACTTATTAAGGAATTTGAAATTGGCTTTGCTGATAAAGATAAAAATTTGTGTAATATGCTAATTGCTAAAAATTGTTCATCATTTAATTTAGCCAATTCGTCATTGATAGCAAGCAACAATTTTGATAACTTTTTTAATGATAGATTGATGTTTCCAATCAAAAATAAGGATGGAAGAATTGTTGCTTTTTCTGGAAGAAGTATTTATAATAACGTTGAACCAAAATATTTAAATAGTTCTGAAACAATAGTTTTTAAAAAACATGAAGTTATGTTTAATTACTATCATGCATTGGATGAAATTATAAAGAAATCAAGTGTTTATTTAGTTGAGGGGCAATTTGATTGCATTGCTCTTTATAAAATAAACATAAAAAATGCAGTAGCACTTTTAGGCACAGCTTTATCTATACAAAATTTAGAATCTTTAAGAAATTGTAATATTGTTTTGTTTTTTGATAATGATTTTGCCGGCAAAAATGCTACTAAGAAAAATTTAAGAATAATATTAGCAAATCAAAAAAGATATAATTTAAAAGTTTCTTTTGTTTTGAATGAACATAGCAAAGATCCTGACGAATTATATAATATTGACGAAGGAAAAACATTGTCTGAGATAGCATGTTGTCCTGTCGATTTATTTATGTATTTATTCAATGAATTTAAACAAGTATTTCAATTGGAAACCAACGAATCAAGAAAGGTTGAATTAAATGCTGAATTGTTTGAATTTGTGATTTATTTGCAAGAACCATTAATATTGAAATTAAAAGAAAAATTGATTGAAAATAAAATTTTTTCAATTGAAACTTGA", "aa": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIANDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSIET", "aa_hexdigest": "7fc114e5b04fdbc2749a0a327458ce83", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaG" ], "truncated": "5-prime", "pseudogene": { "start": 284790, "stop": 286352, "observations": { "mutation": [ 285095 ], "stop": [ 285095 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J803", "query_cov": 1.0, "subject_cov": 0.6768982229402262, "identity": 0.998, "score": 813.0, "evalue": 5.5e-294, "gene_start": 1, "gene_end": 419, "reference_start": 1, "reference_end": 419, "reference_sequence": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIANDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSVETWNKYLFLYGKENFPLHPISKNNLKPSLDNEYNKKNYSKNSNYKKYEDLNFAYSDPSFDRLDFFEEPLIQQPKLITGRNLVFYLIIKTIIKHPQYIANYQLVDFARMAFSNGCQNHKMFLCFLINSIKNNKSIDETNFESYITNNIDLEDNTKQIYLDLFKEIMSLKNKEYTKEELDKNLNNWMKETGQSKDLIINLDRRK" }, "paralog": false, "identity": 0.9942307692307693, "score": 1032.0, "evalue": 0.0, "description": "Internal stop codon at 285095. Nonsense mutation around 285095.", "psc": { "uniref90_id": "UniRef90_D1J803", "gene": "DnaG", "product": "DNA primase", "ec_ids": [ "2.7.7.101" ], "uniref50_id": "UniRef50_D1J803", "cog_id": "COG0358", "cog_category": "L", "kegg_orthology_id": "K02316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J803", "EC:2.7.7.101", "UniRef:UniRef50_D1J803", "COG:COG0358", "COG:L", "KEGG:K02316" ] }, "pscc": { "uniref50_id": "UniRef50_D1J803", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J803" ], "product": "DNA primase" } }, "id": "LMJBGEANGM_528", "locus": "GCA013368015_00505" }, { "type": "cds", "sequence": "contig_1", "start": 286363, "stop": 286881, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "TTGCTTGGTATTGCAAATCGTGGCAATTATGATTTAAACCAACATATGAAATATTCAAATGAATCTCTTGAATATTTGTTAGACGATGGAACTAAAATAATTCCTCATGTTATTGAACCTTCAATTGGTTTAGATCGTTTATTGCTTGCGTTGCTTGTAGATTCATATGAAGAACAAGAACTAGAAAATAATGATGTAAGAATTGTTTTAAAACTTGATTTCAATATTGCACCATATCAAGTTGCGATTTTTCCATTAATGAAAAAACTTAACTCTAAGGCTAAAGAAATATTTGATAATTTATTGCAAAAAACTGAATTAAGATTAATTTATGATGAAAGCGGTTCGATAGGAAAACGTTACCGTCGTCAAGATGCAATTGGTACACCATTTGCAGTAACAATAGATTTTGACACATTAGAAAAAAATACAGTAACTGTCAGAGAACGCGATTCTATGAAACAACATGTAGTTGAATTAGACAACTTAATTAAGTTTTTTGAAAATTATAAAAAATAA", "aa": "MLGIANRGNYDLNQHMKYSNESLEYLLDDGTKIIPHVIEPSIGLDRLLLALLVDSYEEQELENNDVRIVLKLDFNIAPYQVAIFPLMKKLNSKAKEIFDNLLQKTELRLIYDESGSIGKRYRRQDAIGTPFAVTIDFDTLEKNTVTVRERDSMKQHVVELDNLIKFFENYKK", "aa_hexdigest": "02bdd4a64f2e0526a81bec7d45d2bddf", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.9651162790697675, "subject_cov": 0.9707602339181286, "identity": 0.657, "score": 228.0, "evalue": 7.7e-74, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.5232558139534884, "hmm_cov": 1.0, "evalue": 1.444546937337835e-23, "score": 78.41000366210938, "start": 80, "stop": 169 } ], "seq_stats": { "molecular_weight": 20011.69619999999, "isoelectric_point": 5.293097114562988 }, "id": "LMJBGEANGM_529", "locus": "GCA013368015_00506" }, { "type": "cds", "sequence": "contig_1", "start": 287026, "stop": 287292, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "nt": "ATGTTTAAAACCCAAATGGGTGCAATTGATACAGCGGCAAATCAAACATATTTGCGTCCTGAAACAGCCCAAGGAATTTTTATAAATTTTAAAAATGTCTTAAGAACAACTAGAGCTAAGTTACCTTTTGGAATTGGCCAAATTGGTAAAAGTTTTAGAAATGAAATTACACCTAGGGATTTTATTTTTAGAACAAGGGAATTCGAACAAATGGAATTAGAATATTTCTGCAATCCTAGTGAAGATGAAAAGTTTTACCATTATTGA", "aa": "MFKTQMGAIDTAANQTYLRPETAQGIFINFKNVLRTTRAKLPFGIGQIGKSFRNEITPRDFIFRTREFEQMELEYFCNPSEDEKFYHY", "aa_hexdigest": "fb21550e42e24642fc3daa802e4e2712", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003241E146", "query_cov": 0.9318181818181818, "subject_cov": 0.8913043478260869, "identity": 0.695, "score": 123.0, "evalue": 5.97e-35, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_X1KVU4" }, "pscc": { "uniref50_id": "UniRef50_X1KVU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_530", "locus": "GCA013368015_00507" }, { "type": "cds", "sequence": "contig_1", "start": 287581, "stop": 287739, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAAATACAAAAAAATATTCAAAATATAATAAATCACTTAAAAGTTTCTGGTTTTGTTTATCAAAATTCAGAAATCTATGGAGGCTTAATAAACACCTGGGATTATGGGCCACTAGCAGTTAATATTATGACTGCGATTAAGAAATATTGA", "aa": "MGDKIQKNIQNIINHLKVSGFVYQNSEIYGGLINTWDYGPLAVNIMTAIKKY", "aa_hexdigest": "060c8f592c19fc45fda4a41c098dc6d2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5915.793100000001, "isoelectric_point": 9.10114688873291 }, "id": "LMJBGEANGM_531", "locus": "GCA013368015_00508" }, { "type": "cds", "sequence": "contig_1", "start": 287884, "stop": 289212, "strand": "+", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "RefSeq:WP_012855495.1", "SO:0001217", "UniParc:UPI0001BC21DF", "UniRef:UniRef100_D1J805", "UniRef:UniRef50_A0A448ZXZ7", "UniRef:UniRef90_D1J805" ], "nt": "ATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAATTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCGAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAAAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCCGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACAGATTTCTTTACTATGGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAGGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATCACAAAATTAGATAGTGATGCTCGTGGTGGCGCAGCATTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATGGGCGATGTTCTAACATTAATTGAAAAAGCCTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTGATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCTGGAATGGCAGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTTGAGAAAAAAAACCCACGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAAGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAACAAATGAAAGAATTGGCAAACGGAAATCTAAAATTAAAAAATGGCTTGGATGAATAA", "aa": "MLDFIQKRMQNSIQKINKKMSINEEDILEILREIKISLLEADVNLEVVKNFTKEVKAKALSSQIIGKLNQQQTVLKIFKDELTRILGSKSIDIKTNNNPTKIMMVGLQGSGKTTTAAKLSVYFKKKNLAVNPLLVGDDIYRPAAREQLQQLAKQTQTDFFTMEENNAINIAQKAIELAKENKNDLVIIDTAGRLAIDENLMQELKDIKKYTHPDYIFLVVDAMSGQDVINTAKIFHQELNLDGTIITKLDSDARGGAALSITHLLNVPIIFIGTGEKLTGLELFHPDRMADRILGMGDVLTLIEKASEEVDEKMMKKIGYKMLSGQFNLMDLMNSLAQIKKLGKMKAILRMIPGMADKVSDEKIEEAENKFRIYTYLINSMTNFEKKNPRVLKNPSRKQRILQGSGRSPREFNMLISDFERMSKQMKELANGNLKLKNGLDE", "aa_hexdigest": "5f3bd5f4930eef166ea57925f9607a43", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC21DF", "ncbi_nrp_id": "WP_012855495.1", "uniref100_id": "UniRef100_D1J805", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21DF", "RefSeq:WP_012855495.1", "UniRef:UniRef100_D1J805" ] }, "ips": { "uniref100_id": "UniRef100_D1J805", "uniref90_id": "UniRef90_D1J805" }, "psc": { "uniref90_id": "UniRef90_D1J805", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A448ZXZ7", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXZ7" ], "product": "Signal recognition particle protein" }, "genes": [ "ffh" ], "id": "LMJBGEANGM_532", "locus": "GCA013368015_00509" }, { "type": "cds", "sequence": "contig_1", "start": 289334, "stop": 289669, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAACAATCAATTTCATTTTAAATACTTATTTTTCAAAACAAAATTGTCAAGAATTAAAACCTCGCGATAAAAAAATAGCCTCTGAAAATATCAAATATATTTTAGAAACATTTAAAAACCATTCATATTTTTTAAATAATTTATTTAATCACTTCATCAATAAAATTAAAACTATTTCTCCATATAACCTAATTGTAAAAACTAAAAAATTAAATTCAATATTTAAATTAAGAAATATTATTATTTTTGATAGATATGTTTTATCAAGCTTAAAAATATCAAAAGCAATATTAGTCTTAATTTCAATTAAAAATAAGAATAAATTATAA", "aa": "MQETINFILNTYFSKQNCQELKPRDKKIASENIKYILETFKNHSYFLNNLFNHFINKIKTISPYNLIVKTKKLNSIFKLRNIIIFDRYVLSSLKISKAILVLISIKNKNKL", "aa_hexdigest": "ec6edd12996e898096a0190c5c3803b2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13213.552599999974, "isoelectric_point": 10.082484626770018 }, "id": "LMJBGEANGM_533", "locus": "GCA013368015_00510" }, { "type": "cds", "sequence": "contig_1", "start": 289751, "stop": 289909, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTATTTTCGTTCAACGATAATCAAAAATATATAGAAATTAAGAAATTTTTGTCTCAATTGAATACATTTAAGGAATTTATTTATTTTATAGTAGAAACACTTTTAAACTATAGCGATATTTATGCGAAATTAAAAACCTTTGATGAATTATGA", "aa": "MFLFSFNDNQKYIEIKKFLSQLNTFKEFIYFIVETLLNYSDIYAKLKTFDEL", "aa_hexdigest": "bf67931aa5b4ac998666b296d549052f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6415.358999999999, "isoelectric_point": 5.0959794998168935 }, "id": "LMJBGEANGM_534", "locus": "GCA013368015_00511" }, { "type": "cds", "sequence": "contig_1", "start": 290054, "stop": 290218, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGCATTTTTAAGCAACGAAACAAATAGCAAAGCTATATTTTTAACATTATTTAAAGATGGAAAAATTGATGATATATTATTTAGATTACAATCAAGACTTGAAGATGTATTTTTAACTAAAAAAACAATTATTAATTTTGATTTGCGTGAACAATGA", "aa": "MIDAFLSNETNSKAIFLTLFKDGKIDDILFRLQSRLEDVFLTKKTIINFDLREQ", "aa_hexdigest": "95c5dd58ad2fb7a5d4869a552ea8e061", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6366.297299999998, "isoelectric_point": 5.125876808166503 }, "id": "LMJBGEANGM_535", "locus": "GCA013368015_00512" }, { "type": "cds", "sequence": "contig_1", "start": 290495, "stop": 290635, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGAAAACTTATTTAGTTGGTTTTGCACCGAAAAAGGATATAAAAAAGTTGCATATGATTTACTTGCTAAATTAAGTTTAACTAGTGAATTTAAAATGTCTAATTTAATTCAATATTGTAGTGATTCTAAATATTGA", "aa": "MYENLFSWFCTEKGYKKVAYDLLAKLSLTSEFKMSNLIQYCSDSKY", "aa_hexdigest": "b94e185e54499b140b2c93947f887f8c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5488.2883, "isoelectric_point": 7.727511024475096 }, "id": "LMJBGEANGM_536", "locus": "GCA013368015_00513" }, { "type": "cds", "sequence": "contig_1", "start": 290654, "stop": 291007, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTATAAATTAACTAATTTAATAACAGCAAAAAATGAAACAAAAACCAATTTAATTATTGAAATAAAAAAAGATTTTGAAAATATCGGCAAAGAAATTATTTCAAAATATCAAAAGCTTGTTGAATATATTCAAAAAATTAATCCACTAGCAAATATCGTGGTAACAAATTATGCCAAACCATTTATGGCCTTGCAAGATTTAATTAATTCGATTTACATTTCTTCTTCATATAACAATGAATTTGATTTGGTTGAAATGATACAAGATTTATTAAACAATGTCGCTAAAGAAGTAGCTAAAAAATGCCAATGTTTATATGTAGATATTTATGAAGAAAAATATTGA", "aa": "MPFYKLTNLITAKNETKTNLIIEIKKDFENIGKEIISKYQKLVEYIQKINPLANIVVTNYAKPFMALQDLINSIYISSSYNNEFDLVEMIQDLLNNVAKEVAKKCQCLYVDIYEEKY", "aa_hexdigest": "2fdcb62bf5101cca12f7ae524fa106fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13697.794399999992, "isoelectric_point": 5.486008644104003 }, "id": "LMJBGEANGM_537", "locus": "GCA013368015_00514" }, { "type": "cds", "sequence": "contig_1", "start": 291104, "stop": 291469, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED", "UniRef:UniRef90_UPI001F5209ED" ], "nt": "ATGGCTAAAAATCAAAAATACAATTTAAATGACGAAATTAAATATGTTGCTTTAGGAGATTCATTTGCAGCGGGATTTAATAGCAAAGTAGGGTTTAATACTAATGGAAAATTATCAAATGGTAATATAGATGGTTTGGGTTATCCATCATTTTTAGCCTTAAAATTGCGAGATTTAAACTATAGATTAACAAGTTTTTACAATCTAGCAATACCAGCAGGTAATTTAGATATAATTTATGCTTTAACTTCAAATAATGAAAATGATTTAATTGCCAATTCAAAAAGTCTTGATTTGCTTCAATCAATTGATTGGCATGTTTCAAATCCTTCAAAGAATTTTTTTGATGAATTTTTTTTAAATTGA", "aa": "MAKNQKYNLNDEIKYVALGDSFAAGFNSKVGFNTNGKLSNGNIDGLGYPSFLALKLRDLNYRLTSFYNLAIPAGNLDIIYALTSNNENDLIANSKSLDLLQSIDWHVSNPSKNFFDEFFLN", "aa_hexdigest": "e9610aedb459f1cb65780fab95cbbf46", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001F5209ED", "query_cov": 0.9834710743801653, "subject_cov": 1.0, "identity": 0.992, "score": 238.0, "evalue": 9.53e-80, "valid": true, "uniref50_id": "UniRef50_UPI001F5209ED" }, "pscc": { "uniref50_id": "UniRef50_UPI001F5209ED", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13508.941999999979, "isoelectric_point": 5.16594829559326 }, "id": "LMJBGEANGM_538", "locus": "GCA013368015_00515" }, { "type": "cds", "sequence": "contig_1", "start": 291579, "stop": 291794, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAACTAAAAGATGAAGAAACCGGCGCCAAATTAGCTGTAGTTAGTTCAATAAAGTTAGAAGCTTTAAATATAAATGAAGTTATTGTTGCTACTTACCGCCTAATATCTAAGTATTATCCTCAAAAAATTATTGATATAAAATTGCAACCAAGTCAAGATGAAGAAGAAATCTTTGGCCAATTGAGTTTATATGCTAAAAATAACAATTGA", "aa": "MPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFGQLSLYAKNNN", "aa_hexdigest": "5522c1addd8439a2a08dfb1240301d01", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8068.2095999999965, "isoelectric_point": 5.311001396179199 }, "id": "LMJBGEANGM_539", "locus": "GCA013368015_00516" }, { "type": "cds", "sequence": "contig_1", "start": 291840, "stop": 292289, "strand": "-", "frame": 1, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_A0A077L649", "UniRef:UniRef90_A0A454CAF5" ], "nt": "ATGGCCAAAACCATTAGATTATTAAATGAAAAAATAAGATTATTTGATTTATTTATAATTGTTTTAGATGCACGCTTACCTCTTACTTCGTTTAATAACGATATTTATAAGATGGCTAATAATAAGCCAATTTTATTTATATTAAACAAAAGCGACAAAGCAGATCTATCTAAATTAAAACCTTTTATAAATGAATATGAGAAGAAAGGTGTTGTTGTAACAACTAATTTGAAAGACAAATTAGCCTATAAAAAAATAAATTCAGCTCTTAATAATATTTATTTAAAAGTAAAAGAAAAAAATGCACAGAAACATAAGCTAACACCCGCATTAAAATGTGTCGTGCTTGGCGTTCCTAACGTTGGTAAAAGTACCTTAATAAATTTATTGGCTGGTTCCAAAGTTGCAAAAGTGGGTGCAATGGCAGGAGTAACAAAGTCAGAGCAATGA", "aa": "MAKTIRLLNEKIRLFDLFIIVLDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQ", "aa_hexdigest": "5210e832a85ee2d2043f177ad2b5f1f3", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "both", "pseudogene": { "start": 291537, "stop": 292313, "observations": { "mutation": [ 291581, 291842, 292304 ], "stop": [ 291581, 291842, 292304 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CAF5", "query_cov": 1.0, "subject_cov": 0.5173611111111112, "identity": 0.993, "score": 278.0, "evalue": 2.49e-92, "gene_start": 1, "gene_end": 149, "reference_start": 9, "reference_end": 157, "reference_sequence": "MFNWFPGHMAKTIRLLNEKIRLFDLFIIILDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQWINCKNYLLLDTPGLLMPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLSLYAKNNNWILKNQLPDIKKAIVQLISYFNNLNNIVYDQCQEKEQNGWKSKI" }, "paralog": false, "identity": 0.9806201550387597, "score": 488.0, "evalue": 7.94e-178, "description": "Internal stop codon at 291581, 291842, 292304. Nonsense mutation around 291581, 291842, 292304.", "psc": { "uniref90_id": "UniRef90_A0A454CAF5", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_A0A077L649", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CAF5", "UniRef:UniRef50_A0A077L649", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L649" ], "product": "Ribosome biogenesis GTPase A" } }, "id": "LMJBGEANGM_540", "locus": "GCA013368015_00517" }, { "type": "cds", "sequence": "contig_1", "start": 292322, "stop": 292537, "strand": "-", "frame": 2, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAACTGAAAGTACTGGAATTCCTAAGAGAATTATGCAAACAAATATTCCTAATTGTTTGCGTATCCCAATGTTTGATGAATGTCGTAGTTTAAATTTAGCCAATAGTGTTGCAATAGTACTATTTGAAGTATTAAGACAAAATGATTTCGCCGGACTATCAAAATTTGAAAGTCAAAAAGGGAAGGATTTTATTTTAAGGAAAGATTGA", "aa": "MFGTESTGIPKRIMQTNIPNCLRIPMFDECRSLNLANSVAIVLFEVLRQNDFAGLSKFESQKGKDFILRKD", "aa_hexdigest": "6bd8369e0e35ce00d282458f1ad0d93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 1.0140845070422535, "subject_cov": 0.8674698795180723, "identity": 0.514, "score": 79.0, "evalue": 7.73e-18, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "LMJBGEANGM_541", "locus": "GCA013368015_00518" }, { "type": "cds", "sequence": "contig_1", "start": 292541, "stop": 292720, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAGGCTGCTGGACACTTTTTAACTGAAATTCAACATGAAGTTCATGAAAATTATGATGAATTTTTTAAAAAATATGGTGATAAAAATATTTATTATGTAACAAGATACGGTTTAAAAAATTACGTAGATGTTGATTACAAACAAGAATTGCAAACAAATGGTGAACTTTGA", "aa": "MKRKAAGHFLTEIQHEVHENYDEFFKKYGDKNIYYVTRYGLKNYVDVDYKQELQTNGEL", "aa_hexdigest": "274fd3c15d6e6c613318b320804565af", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7178.914400000005, "isoelectric_point": 6.257200050354004 }, "id": "LMJBGEANGM_542", "locus": "GCA013368015_00519" }, { "type": "cds", "sequence": "contig_1", "start": 293218, "stop": 293592, "strand": "+", "frame": 1, "gene": null, "product": "Helix-turn-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "nt": "ATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGCTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCAAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAATTTTCAAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGA", "aa": "MNYNIHKYFHLSLEERFFIQRSLENNLSLRAISRQLNRSASTISREIKRNSNFNEYIFLRADKKSKFRAYHKYMFKFNINFEFEEFSKIFKSKYDKKYFGVKATMNYIEENFNVKKPTMRTVFN", "aa_hexdigest": "c57bd1123260a439ee4f69e485682c4a", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0AAJ1UWR4", "query_cov": 1.0080645161290323, "subject_cov": 0.8116883116883117, "identity": 0.6, "score": 141.0, "evalue": 1.13e-40, "valid": false, "product": "Helix-turn-helix domain-containing protein", "uniref50_id": "UniRef50_A0AAJ1UWR4" }, "pscc": { "uniref50_id": "UniRef50_A0AAJ1UWR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ1UWR4" ], "product": "Helix-turn-helix domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_543", "locus": "GCA013368015_00520" }, { "type": "cds", "sequence": "contig_1", "start": 293776, "stop": 294057, "strand": "+", "frame": 1, "gene": null, "product": "IS30 family transposase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "nt": "TTGGTTCTTGGAAAAAGAGCAAATGGCTATAAGAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGTGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGA", "aa": "MVLGKRANGYKNILTLTERKTRVGFATFVSSKSPFEINSKIKELIKNKNLIVKTITIDNGIEFEKIGFLAKSLNIIIYRVEPYASFQRGSNEN", "aa_hexdigest": "5be3003d06eb2b3f6fa76e6c2f892a61", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F20DEBE", "query_cov": 0.978494623655914, "subject_cov": 0.8272727272727273, "identity": 0.659, "score": 124.0, "evalue": 3.06e-35, "valid": false, "product": "IS30 family transposase", "uniref50_id": "UniRef50_UPI001F20DEBE", "go_ids": [ "GO:0004803" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F20DEBE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F20DEBE" ], "product": "IS30 family transposase" }, "genes": [], "id": "LMJBGEANGM_544", "locus": "GCA013368015_00521" }, { "type": "cds", "sequence": "contig_1", "start": 294064, "stop": 294174, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGA", "aa": "MIRREYQKGFNFNILTQEDLDTTIAKINNMPREILN", "aa_hexdigest": "8ef7b9776e982e4522979ce89f78742f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4340.9349, "isoelectric_point": 6.038881492614746 }, "id": "LMJBGEANGM_545", "locus": "GCA013368015_00522" }, { "type": "cds", "sequence": "contig_1", "start": 294402, "stop": 294668, "strand": "-", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855499.1", "SO:0001217", "UniParc:UPI0001BC21E3", "UniRef:UniRef100_D1J809", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_D1J809" ], "nt": "ATGGTATCAAAAGAAAAAAAAGCTGAATTAGTTGTTAAATTCGGACGTAATGCAAAAGACACTGGATATTTACCAGTACAAATAGCAATTTTAACTGAAGACATTGAAAGTTTAAAAGAACATTTCGCAGTAAATAAAAAAGATTTACATTCAATGAGAGGATTCATTGCTAAAGTTAATCACCGTAAAGCATTACTAAACCACTTAAAAGACGAAAACTACAACTTATATTTAGAAACCATCAAAGCTTTAAACATCAGAAAATAA", "aa": "MVSKEKKAELVVKFGRNAKDTGYLPVQIAILTEDIESLKEHFAVNKKDLHSMRGFIAKVNHRKALLNHLKDENYNLYLETIKALNIRK", "aa_hexdigest": "fbc082619cd1a86501261f37c1edf520", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC21E3", "ncbi_nrp_id": "WP_012855499.1", "uniref100_id": "UniRef100_D1J809", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E3", "RefSeq:WP_012855499.1", "UniRef:UniRef100_D1J809" ] }, "ips": { "uniref100_id": "UniRef100_D1J809", "uniref90_id": "UniRef90_D1J809" }, "psc": { "uniref90_id": "UniRef90_D1J809", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "LMJBGEANGM_546", "locus": "GCA013368015_00523" }, { "type": "tRNA", "sequence": "contig_1", "start": 294791, "stop": 294867, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 83.1, "nt": "GCGAGCGTAGCTCAGTTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 294831, 294833 ], "id": "LMJBGEANGM_31", "locus": "GCA013368015_00524" }, { "type": "cds", "sequence": "contig_1", "start": 294916, "stop": 295314, "strand": "-", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_012855500.1", "SO:0001217", "UniParc:UPI0001BC21E4", "UniRef:UniRef100_D1J810", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_D1J810" ], "nt": "ATGGCAGATAAAGTAAGATACTATGGTTTAGGTCGTCGTAAATCTTCAGTAGCAAGAGTTTATATTTTACCAGGTAGTGGAAATTTAATTATTAATGGAATAGTTGCAAAAGAATACTTAAATTCAGAAATTTTAATAAAAGATGCTTTAAGTCCTTTTGCAGTAACTGAAACTGCTAACCAATTTGATGTTTTTGCAAATGTTAATGGTGGTGGTTTAACAGGACAAGCTGGAGCAATTAGATTAGGTATTGCTAGAGCATTATTAGAAGCATCTAATAATGAATATAGAAACAAATTAAAGGATGCAGGATTTTTAACACGTGATGCTAGAGTTAAAGAACGTAAGAAATTCGGTTTAAGAAAAGCAAGACGTGCAAGACAATTTTCAAAACGTTAA", "aa": "MADKVRYYGLGRRKSSVARVYILPGSGNLIINGIVAKEYLNSEILIKDALSPFAVTETANQFDVFANVNGGGLTGQAGAIRLGIARALLEASNNEYRNKLKDAGFLTRDARVKERKKFGLRKARRARQFSKR", "aa_hexdigest": "8a2d74830272a058243860cc79a3c920", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC21E4", "ncbi_nrp_id": "WP_012855500.1", "uniref100_id": "UniRef100_D1J810", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E4", "RefSeq:WP_012855500.1", "UniRef:UniRef100_D1J810" ] }, "ips": { "uniref100_id": "UniRef100_D1J810", "uniref90_id": "UniRef90_D1J810" }, "psc": { "uniref90_id": "UniRef90_D1J810", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "LMJBGEANGM_547", "locus": "GCA013368015_00525" }, { "type": "cds", "sequence": "contig_1", "start": 295317, "stop": 295601, "strand": "-", "frame": 1, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGCGACAATGTGGTTGTGATCAATGCTAAAGATGTACTTTTAACTGCTAAAAAAGATGAAATAAAAGTATATTACCACCACACTGGATACCCAGGTGGATTAAAACAAATTACTGCTGCTGATTTAAGAGCAAAAAAACCAGAATTATTAGTTGAAAAAGCTGTTAGAGGAATGCTTCCACACACCAAATTAGGTCGTAAACAATTTAAAAATTTATATGTTTATGCAGGTTCTGAACACAAACAAGAAGCACAACAACCAGTTAAATTAGAGGTTAAATAA", "aa": "MGDNVVVINAKDVLLTAKKDEIKVYYHHTGYPGGLKQITAADLRAKKPELLVEKAVRGMLPHTKLGRKQFKNLYVYAGSEHKQEAQQPVKLEVK", "aa_hexdigest": "5376e3427d2b3b0ced82ebaa8fcaf930", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.649, "score": 124.0, "evalue": 3.48e-35, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "LMJBGEANGM_548", "locus": "GCA013368015_00526" }, { "type": "cds", "sequence": "contig_1", "start": 295864, "stop": 296526, "strand": "-", "frame": 3, "gene": "rsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "COG:COG1385", "COG:J", "EC:2.1.1.193", "RefSeq:WP_113248154.1", "SO:0001217", "UniParc:UPI000DDC318C", "UniRef:UniRef100_UPI000DDC318C", "UniRef:UniRef50_A0A448ZY27", "UniRef:UniRef90_D1J812" ], "nt": "ATGTACAAATTTTTTGCTAACAAATTAGAAGATGGAATTTTTTATTTTAGTGATGAAATTCAAAAGCATTTTAAGGTTTTGCGCTTTAATAAAGAAAATATTATTGTAAATTATGAAAATAAGTTTTATGAATGTCAATTTGTTTTTCCTAATAGTGCTAAATTAGTTAGAGAGTTAGATATTAATAATGAACTTGACTATAAATTATGTGTTGCAATTCCTTTAATTAAACAAAATCATTTTGAAGTAGCATTGCAAAAAGCAGTTGAACTTGGCGCTTATGAAATTTATGTATTTCAAAGCCAATATGTTGATAAATCTAATTTAACATTTGCTTCTAAAAAACAACGTTATGAAAAAATAATTTTCGAAGCTTGCCAACAAAGTTTTAGAAATTGTATCCCAAGACTTCATGATCCAATTTCATTTTCAGAACTTTGTGTCTTGGATATTAAAAATAAAGTATTAGCTTATGAAAACAAAAAAAATAACGAATTAATTAAAGATTGCCAAGATGCTTTAATTATTGTTGGCCCTGAAGGCGGATTTAGCCAACAAGAAATTGATTATGCTATATCTTGCAATGTTCAAGTTGTTTCACTAACAAAAACGATTTTAAGGGCTGAGACCGCTTTAATTTACATGCTTTCAAAATTAAATTAA", "aa": "MYKFFANKLEDGIFYFSDEIQKHFKVLRFNKENIIVNYENKFYECQFVFPNSAKLVRELDINNELDYKLCVAIPLIKQNHFEVALQKAVELGAYEIYVFQSQYVDKSNLTFASKKQRYEKIIFEACQQSFRNCIPRLHDPISFSELCVLDIKNKVLAYENKKNNELIKDCQDALIIVGPEGGFSQQEIDYAISCNVQVVSLTKTILRAETALIYMLSKLN", "aa_hexdigest": "0111eac216fa670b7259b7a40aed1e16", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DDC318C", "ncbi_nrp_id": "WP_113248154.1", "uniref100_id": "UniRef100_UPI000DDC318C", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DDC318C", "RefSeq:WP_113248154.1", "UniRef:UniRef100_UPI000DDC318C" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DDC318C", "uniref90_id": "UniRef90_D1J812" }, "psc": { "uniref90_id": "UniRef90_D1J812", "gene": "RsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A448ZY27", "cog_id": "COG1385", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY27" ], "product": "Ribosomal RNA small subunit methyltransferase E" }, "genes": [ "rsmE" ], "id": "LMJBGEANGM_549", "locus": "GCA013368015_00527" }, { "type": "cds", "sequence": "contig_1", "start": 296719, "stop": 296979, "strand": "-", "frame": 3, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003A3F0D4", "UniRef:UniRef90_UPI0003A3F0D4" ], "nt": "TTGGATAAAGAAACCACAAAAGTTACTATTCCAAACAACATAAAAGAAATTGATGATAGTGCATTTCTTGGCTGTCAAAACCTTAAAAAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGATGAGGCATTTTACAATACAAACATTGAATCTATTACTATTCCTGCTTCAGTAAAAAAATTTGGCTATAGTGCGTTTTTTGGCTGCAAAAACCTTAAAGAAGTAATTTATAAAGGTGATGCAAGTAATATAGGTTGA", "aa": "MDKETTKVTIPNNIKEIDDSAFLGCQNLKKVILNEGLEKIGDEAFYNTNIESITIPASVKKFGYSAFFGCKNLKEVIYKGDASNIG", "aa_hexdigest": "c2e6c3e9f44e3b807cc0d4356cb977f1", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0003A3F0D4", "query_cov": 1.0, "subject_cov": 0.8269230769230769, "identity": 1.0, "score": 118.0, "evalue": 7.5e-33, "valid": true, "product": "leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_UPI0003A3F0D4" }, "pscc": { "uniref50_id": "UniRef50_UPI0003A3F0D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0003A3F0D4" ], "product": "leucine-rich repeat domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_550", "locus": "GCA013368015_00528" }, { "type": "cds", "sequence": "contig_1", "start": 297206, "stop": 297364, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C37BB16", "UniRef:UniRef90_UPI001F525782" ], "nt": "ATGTTTATAGTTTTAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACCAGAAAATGTACTTTTGCCAACTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTATTAAATGCATAA", "aa": "MFIVLNASAPIFSRPSFKITFLRFLQPENVLLPTSFTEPGIVIDSMFVLLNA", "aa_hexdigest": "9bce8767a714b48a86be41aaa6feca4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F525782", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9420000000000001, "score": 94.0, "evalue": 1.77e-24, "valid": true, "uniref50_id": "UniRef50_UPI001C37BB16" }, "pscc": { "uniref50_id": "UniRef50_UPI001C37BB16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C37BB16" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5843.894399999999, "isoelectric_point": 5.937878608703613 }, "id": "LMJBGEANGM_551", "locus": "GCA013368015_00529" }, { "type": "cds", "sequence": "contig_1", "start": 298877, "stop": 300289, "strand": "-", "frame": 2, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9V9", "UniRef:UniRef90_UPI001588D07A" ], "nt": "TTGGATAAAAAAACTTCAAAAGTTACTATTCCAAACAATGTAAAAGAAATCGGTGATTGTGCGTTTTCTGGTTGCAGAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTTGGGCTTTTGAAGATACAAAAATTGAATCTATTACTATTCCTAGTTCAATAAAAGAAATTGGTGATAATGCGTTTTATGGTTGCAAAAACCTTAAAGAAATAATTTTAAATGAAGGTCTTGAAAAAATCGGCTATGAGGCATTTAGAGATACAAACATTGATTCTATTACTATTCCTAGTTCGGTAAAAGAAATTGGAGAAGGCGCATTGTCCAATTCAAATATTTCAAGTATTTTAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTTTTTATTGATAAAAATAATAAAAAGATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCAAACAATGTAAAAGAAATCGGCGATAGTGCGTTTCTTGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAGAATTGGCGCTGAGGCATTTCAAAATCTACCTATCGAGTTGGTTTATATTCCGAGCTCAGTAAAAGAAATTGAGTATAGTTCGTTTTCTAATTGCAAAAATCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGATGCTGATGCATTTTTAAATACAAATATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGTGAAAGTGCTTTTTATGGTTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGCCTTGAAAGAATTGGTGCTAAGGCTTTTTCAGATACAAACATTAAATTAGTTAATATTCCTAATTCAATAAAAATATTAGAAGATTCAGCATTTGATTTTTCAATAATTTTACATAGTTTAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTTTTTATTGATAAAAATAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTGTTATTCCAAACAATGTAAAAAAAATTGGTGAAAGTGCGTTTTCTAATTGCGAAAACCTTAAAGAAGTAAGTTTAAATGAAGGTCTTGAAAAAATTGGTGCAGAGACATTTTATGATACAAAAATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGGCGAAAGAGCGTTTTGTAATTGCGAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTAAAAAAATCGGCGCTAGTGCATTTCCAAATACAAAAATTGAATCTATTACTATTCCAAGCTCGGTAAAAGAAATTGGTGAAAGTGCGTTTTCTAATTGCAAAAACCTTAAAGAAGTAATTTATAAAGGTGATGCAAGTAATATAAATTGA", "aa": "MDKKTSKVTIPNNVKEIGDCAFSGCRNLKEVILNEGLEKIGAWAFEDTKIESITIPSSIKEIGDNAFYGCKNLKEIILNEGLEKIGYEAFRDTNIDSITIPSSVKEIGEGALSNSNISSILINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPNNVKEIGDSAFLGCKNLKEVILNEGLERIGAEAFQNLPIELVYIPSSVKEIEYSSFSNCKNLKEVILNEGLEKIDADAFLNTNIESITIPGSVKEIGESAFYGCENLKEVILNEGLERIGAKAFSDTNIKLVNIPNSIKILEDSAFDFSIILHSLINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVVIPNNVKKIGESAFSNCENLKEVSLNEGLEKIGAETFYDTKIESITIPGSVKEIGERAFCNCENLKEVILNEGLKKIGASAFPNTKIESITIPSSVKEIGESAFSNCKNLKEVIYKGDASNIN", "aa_hexdigest": "b8b3a29295aec10b11ba0c3ec69cc7da", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 298823, "stop": 300589, "observations": { "mutation": [ 298879, 300388, 300460, 300559 ], "stop": [ 298879, 300388, 300460, 300559 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001588D07A", "query_cov": 0.8212765957446808, "subject_cov": 0.4701583434835566, "identity": 0.992, "score": 370.0, "evalue": 1.08e-116, "gene_start": 1, "gene_end": 386, "reference_start": 334, "reference_end": 719, "reference_sequence": "MKNKKNSKIWLILSSISTLTAILPLAAISCDHKVKQNSKENNEDLKPDENKPEPKPENPTKEDLKPDENKPEPKPENPTKEEDSSNLNKTFKFELTKSNTWKIAKYIGNDSEVIIPKEYQGKNITEIGNGAFSNSVIQSVTIPSSIKKIGDYAFEYSQNLKDVVLNEGLEEIGQYAFFGSSLTSVVIPNSTKKIGGAAFDYWNPFINIKNANFEIRHNFLIDKRDRKILAYLDRNATKVVIPTWIKKIGDVAFNRCENIKEVILNEGLEKIGDGAFWNTHIESITIPSSVKEIEECALPTWNISSILIDSNNKNFEIKDNFFIDKNNKKILGYLDKKTSKVTIPNNVKEIGDCAFSGCRNLKEVILNEGLEKIGAWAFEDTKIESITIPSSIKEIGDNAFYGCKNLKEIILNEGLEKIGYEAFRDTNIDSITIPSSVKEIGEGALSNSNISSILINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPNNVKEIGDSAFLGCKNLKEVILNEGLERIGAEAFQNLPIELVYIPSSVKEIEYSSFSNCKNLKEVILNEGLEKIDADAFLNTNIESITIPGSVKEIGESAFYGCENLKEVILNEGLERIGAKAFSDTNIKLVNIPNSIKILEDSAFDFSIILHSLINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVVIPNNVKKIGESAFSNCENLKEVSLNEGLEKIGAETFYDTKIESITIPGSVKEIGERAFCNCENLKEVILNEGLKKIGASAFPNTKIESITIPSSVKEIGESAFSNCKNLKEVIYKGDASNINWKNIGIDKTKIKIISSNN" }, "paralog": false, "identity": 0.9931972789115646, "score": 588.0, "evalue": 6.18e-204, "description": "Internal stop codon at 298879, 300388, 300460, 300559. Nonsense mutation around 298879, 300388, 300460, 300559.", "psc": { "uniref90_id": "UniRef90_UPI001588D07A", "product": "leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_A0A454C9V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001588D07A", "UniRef:UniRef50_A0A454C9V9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9V9" ], "product": "Leucine-rich repeat protein" } }, "id": "LMJBGEANGM_552", "locus": "GCA013368015_00530" }, { "type": "cds", "sequence": "contig_1", "start": 300986, "stop": 301099, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGCCTGATGAAAATAAACCAGAACCCAAACCAGAAAATCCTACCAAAGAAGAAGATTCATCAAATTTAAATAAAACTTTTAAATTTGAACTAACTAAATCAAATACTTGA", "aa": "MKPDENKPEPKPENPTKEEDSSNLNKTFKFELTKSNT", "aa_hexdigest": "f70ad840c4edccb4801e4052c43c697c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4293.675500000001, "isoelectric_point": 5.221025276184081 }, "id": "LMJBGEANGM_553", "locus": "GCA013368015_00531" }, { "type": "cds", "sequence": "contig_1", "start": 301564, "stop": 301653, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATATTAATAAAGAATTTATATTAATGCAGCAAGAGGCTAAATTAAACAATAATTATAGCCTTGTGGATGCATTTGAAAGAAATTAA", "aa": "MDINKEFILMQQEAKLNNNYSLVDAFERN", "aa_hexdigest": "c5464218d1eeb649885d3a75d7c70004", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3488.8987000000006, "isoelectric_point": 4.512527275085449 }, "id": "LMJBGEANGM_554", "locus": "GCA013368015_00532" }, { "type": "cds", "sequence": "contig_1", "start": 302095, "stop": 302643, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "TTGGATAAGGAGGCCACAAAAGTTACTATTCCTGATTCAGTAAAAGAAATTTGCAAAGGTGCGTTTTATGGTTGCAAAAACCTTAAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGTGCCGAGGCATTTAAATATACAAACATTGAATCTATTACTATTCCTGGCACAGTAAAAGAAATTGGCGTTGAGGCATTTCGCCATACAAACATTAAATCCATTACTATTCCTAATTCAGTAAAAGAAATTGGCGAAAGAGCGTTTTTTGGTTGCCAAAACCTTAATGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTAAGGCATTTTATCATACAAGCATTGAATCTATTACTATTCCTGGTTCAGTAAAAGAAATTGACGAAAGTACATTGTCTGGTTGCGAAAACCTTGGAGAAGTAGTTTTAAATGAAGGCCTTGAAAGAATTGGCGATTATGCATTTAGGTATGCAAAAATTTTATTTATTACTATTCCTGGCTCAGTAAAAGAAATTGGCGCAGGTGCGTTTTCTGGTTGCTGA", "aa": "MDKEATKVTIPDSVKEICKGAFYGCKNLKEVILNEGLEKIGAEAFKYTNIESITIPGTVKEIGVEAFRHTNIKSITIPNSVKEIGERAFFGCQNLNEVILNEGLEKIGAKAFYHTSIESITIPGSVKEIDESTLSGCENLGEVVLNEGLERIGDYAFRYAKILFITIPGSVKEIGAGAFSGC", "aa_hexdigest": "2fd61f0acd083116c4de0d40d23df0e6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 123, "aa_cov": 0.6758241758241759, "hmm_cov": 1.0, "evalue": 3.5286623513135526e-58, "score": 190.57095336914062, "start": 60, "stop": 182 } ], "seq_stats": { "molecular_weight": 19755.42509999999, "isoelectric_point": 5.115191078186036 }, "id": "LMJBGEANGM_555", "locus": "GCA013368015_00533" }, { "type": "cds", "sequence": "contig_1", "start": 302740, "stop": 302949, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "TTGGACAAGAAGGTCACAAAAGTTACTATTCCTGATTCAGTAAAAGAAATTGGCAAAGGTGCGTTTTCTAATTGCAAAAACCTTAATGAAGTAAGTTTAAATGAAGGTCTTGAAAAAATTGGTGATGAGGCATTTTCAAGTACAAAAATTGAATCTATTACTATCCCTGGTTCAATAAAAGAAATTGGCGAAGGTGCATTATCTTTTTGA", "aa": "MDKKVTKVTIPDSVKEIGKGAFSNCKNLNEVSLNEGLEKIGDEAFSSTKIESITIPGSIKEIGEGALSF", "aa_hexdigest": "582cc4bff5b85ebbc0ac2a3cf34d0053", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 66, "aa_cov": 0.9565217391304348, "hmm_cov": 1.0, "evalue": 9.197687880705718e-22, "score": 72.86372375488281, "start": 3, "stop": 68 } ], "seq_stats": { "molecular_weight": 7344.283299999997, "isoelectric_point": 5.022429847717285 }, "id": "LMJBGEANGM_556", "locus": "GCA013368015_00534" }, { "type": "cds", "sequence": "contig_1", "start": 303283, "stop": 303612, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAATCAAAAAAAATTTTGTTGACCATGGGGGGGATACTTCCCTTGGTTATTTTAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCCAAACAAACCAGAAGTTAAACCAGAGGATCCAAAGAAACCAGAAGGTGAAAAACCTGGCACAACTCCTGAAGTTAAACCAGAGGATCCAAAGAAACCAGAAGGTGAAAAACCTGGCACAACTCCTGAAGTTAAACCAGAGGATAAACCCGAACCAAAACCAGAAAATCCTACCAAAGAAGAAGATTTATTAAATTTTAGGAAAAATTTTAAATTTGAACTAACTAAATCAAATACTTGA", "aa": "MKKSKKILLTMGGILPLVILAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPEDPKKPEGEKPGTTPEVKPEDKPEPKPENPTKEEDLLNFRKNFKFELTKSNT", "aa_hexdigest": "4a55a37e24a6ea22671e53e4024aef23", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12063.875799999983, "isoelectric_point": 8.57186145782471 }, "id": "LMJBGEANGM_557", "locus": "GCA013368015_00535" }, { "type": "cds", "sequence": "contig_1", "start": 303888, "stop": 305033, "strand": "-", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0003924", "GO:0005525", "RefSeq:WP_175936711.1", "SO:0001217", "UniParc:UPI00158C0EE6", "UniRef:UniRef100_UPI00158C0EE6", "UniRef:UniRef50_A0A3L9ZYL3", "UniRef:UniRef90_D1J814" ], "nt": "ATGGCAAAAGAATTAAATAATTATAACCCGGTGGCAAAAATAATTGTTATTGGAGTAGGTGGTGGTGGAAATAATAGTGTTGAAACTATGATAAATAGCCATTTAGATAGTTTTCAAATTATTGCTGCCAATACCGATAAACAAGTATTAGCAAAATTTCCTCAAGAATGCGTTTTACATTTAGGTGATGAACGTGGAATTGGCGCGGGTGCTAACCCTGAAATTGGCAAAACTGCTGCTGAATCTTCGCGTGAAGAAATCAAAAGTAGATTGCAAGGAGCTGATCTAGTAATTATAACTGCAGGAATGGGCGGTGGAACCGGCACAGGTGCAGCGCCCGTGATTGCACAAATAGCCAAGGAATGCAATGCTTTAGTAGTTGCAGTTGTAACTACTCCGTTTGACTTTGAAGGTCCAAAGAGAATGAGAATTGCAAAGCAAGGCTTACAAGAAATTAAAAAATGTGTTGATTCATATATTCTTATTTCAAACAATAAACTATTACAACAATATGGAAATATTTCTTTTTCAGATGCATTTATTTGCGCAAATAATGTCTTAAAGCAAACTATTAGAACAATAGTTGATGTTATTGCCACCCCAAGCATTATTAATCTTGATTTTGCAGATTTATCAACCATTATTAAAAATAAAGGTGAAACAGTAATCGGCATTGGCCAAGCAAATGGCCAAGATCGGGCTGTAAAAGCAATCACATCTGCAATAACAAGCCCAATTTTAGAATCAAGTGTTGTTGGGGCTAGTGATGCAATAGTAAATTTCAGTGCTTCTCAAAAGGTAACTTTAAATGAAATTCAAAGTGCTCTGGGAGCTATGCGGGAAATTGTTGGTAATGAAATTAACATTATTTTTGGTATAACAACTCTAGAATCTGAAGAAAGCAACAAATTAGGTGAGCTATTTGTCTCGGTAATCGCCACGGGATTAAGAAAAGACGCTCCTAAAGACATTGATCAAATTCAAGACGAAGTAATCAATGTAATAAAAAAAGACGATTTAAATTATGTTAACGATGAAACAAAGGAATTCTTTGTATCTGAAGGAACATTTAAAACACAATCATTTTTTTCAATGGATAATGATGAAGATTCAAACGATGAACTTGCAGATATATTAAAACACTAA", "aa": "MAKELNNYNPVAKIIVIGVGGGGNNSVETMINSHLDSFQIIAANTDKQVLAKFPQECVLHLGDERGIGAGANPEIGKTAAESSREEIKSRLQGADLVIITAGMGGGTGTGAAPVIAQIAKECNALVVAVVTTPFDFEGPKRMRIAKQGLQEIKKCVDSYILISNNKLLQQYGNISFSDAFICANNVLKQTIRTIVDVIATPSIINLDFADLSTIIKNKGETVIGIGQANGQDRAVKAITSAITSPILESSVVGASDAIVNFSASQKVTLNEIQSALGAMREIVGNEINIIFGITTLESEESNKLGELFVSVIATGLRKDAPKDIDQIQDEVINVIKKDDLNYVNDETKEFFVSEGTFKTQSFFSMDNDEDSNDELADILKH", "aa_hexdigest": "7434e320833e8e084d5437ad6abd0be3", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158C0EE6", "ncbi_nrp_id": "WP_175936711.1", "uniref100_id": "UniRef100_UPI00158C0EE6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C0EE6", "RefSeq:WP_175936711.1", "UniRef:UniRef100_UPI00158C0EE6" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C0EE6", "uniref90_id": "UniRef90_D1J814" }, "psc": { "uniref90_id": "UniRef90_D1J814", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0A3L9ZYL3", "cog_id": "COG0206", "cog_category": "D", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3L9ZYL3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3L9ZYL3" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "LMJBGEANGM_558", "locus": "GCA013368015_00536" }, { "type": "cds", "sequence": "contig_1", "start": 305041, "stop": 305181, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAATAGATAATATTTTAGAATGTCAAATCAAGCAAAATGCTTTAAATAATGAAATTAGTAGGGAATTATTTACAATTGAAGTTTATAGTAATTCCAAAAAGTATTACAATTTAATGCTAAACGATATAAATTAG", "aa": "MLAIDNILECQIKQNALNNEISRELFTIEVYSNSKKYYNLMLNDIN", "aa_hexdigest": "18d5c39f4a1dba767c77377ba2b8f4bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5438.170000000001, "isoelectric_point": 4.655875205993653 }, "id": "LMJBGEANGM_559", "locus": "GCA013368015_00537" }, { "type": "cds", "sequence": "contig_1", "start": 305440, "stop": 305775, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTAAACTAGTTAAAAAAACTAATATTAAGCAAGTTTATTCTTTAAAAACAATATCAAATGCTAATAGTCTAAGATTGCAAGAAATTTTTAGTAGCAACGATTTAAAAATTGCCAAAATATTTTGCTTAGATAGTTTAAAGACAAATTCAGTTTTGTTTAAAAATTCAAAACAAAAAGTAGCTATTTTTGTTGATGTATTAGATGATAAAGCTAAATTTATTTTAAACATTAATAAATCAAATATAGTAAGCCAAGTATTAGATTTAGGACTAAACAAATTAAAAGCAAGAATGTTACAATGCAATTTATTAAGTACAGAAAGCAATTGA", "aa": "MEFKLVKKTNIKQVYSLKTISNANSLRLQEIFSSNDLKIAKIFCLDSLKTNSVLFKNSKQKVAIFVDVLDDKAKFILNINKSNIVSQVLDLGLNKLKARMLQCNLLSTESN", "aa_hexdigest": "4a69b394779e7889bd7591567e80179f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.710499999983, "isoelectric_point": 9.76794338226318 }, "id": "LMJBGEANGM_560", "locus": "GCA013368015_00538" }, { "type": "cds", "sequence": "contig_1", "start": 305875, "stop": 306108, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7", "UniRef:UniRef90_UPI001E400CB7" ], "nt": "ATGAAAAAAATTATTATTGCCGCATATAAATTTTTAAAAAATAGTATCGAAGTAGAAGTTATGGAAAAAACTAATACTTTATCTTTTTGCATTTATAAAAAAGCAATTAATAATATTTATTTAACTGAAAGCGATATAAAAAATTTTATAAAGCAAACAAAGAATGAAATTCAAAAATTAATATCAGCAAGCATAAATGAAGTTATTGTTATGTTGTCTGAAGATAGCAAATGA", "aa": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSK", "aa_hexdigest": "29c293c4bbf54aa26858a8c72d03686c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 305787, "stop": 306108, "observations": { "insertion": [ 305817 ], "mutation": [ 305877 ], "stop": [ 305877 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E400CB7", "query_cov": 1.0, "subject_cov": 0.7264150943396226, "identity": 1.0, "score": 147.0, "evalue": 2.03e-44, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSKWNINSKLLTLNNDNFNISNLKILDQTSII" }, "paralog": false, "identity": 0.9811320754716981, "score": 192.0, "evalue": 1.06e-64, "description": "Internal stop codon at 305877. Frameshift due to insertion around 305817. Nonsense mutation around 305877.", "psc": { "uniref90_id": "UniRef90_UPI001E400CB7", "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E400CB7", "UniRef:UniRef50_UPI001E400CB7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8904.433299999999, "isoelectric_point": 9.04112682342529 }, "id": "LMJBGEANGM_561", "locus": "GCA013368015_00539" }, { "type": "cds", "sequence": "contig_1", "start": 306209, "stop": 307015, "strand": "-", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0016434", "RefSeq:WP_114091198.1", "SO:0001217", "UniParc:UPI000DED655C", "UniRef:UniRef100_D1J816", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_D1J816" ], "nt": "ATGGAACATATTCCAGTTCTATTAAATGAAGTAATAGAACAATTATCAATAAAACCAGACGGAATATATGTTGATTTAACATTAGGCCGTGGTGGTCATAGCCAAGAAATTTTAAAAAGACTAACAACTGGAAAATTAATTTGTTTTGATAAAGATATTCAAGCTATAAACGAAAGTAGAGAAAAATTAATAAAAATAAGCCAAAACTTTATTTTAATAAAAAGTGATTTTAGAAACATTACTTGTGAACTAAAAAAACTAAATATCGATAAAGTCGATGGAATATTAGCAGATTTAGGAGTTTCTAGTCCTCAATTAGATGATCCAAATAGAGGATTTTCTTATTCAATCAATTCTGATTTAGATATGAGAATGGATTTAGATAATACTCTAACTGCAAAAGAAATTATTAATAATGCAACTGAAAAAGAAATTATAGATATATTGCAATCTAATGCTGATGTAAAATTAAGTAATTTAATTGCGAAAGCTATTGTAAAAAATCGACCAATATCTGATTCATTTACGTTAAATCAAATAATAAAGGATTCATTGCCAGCTAAAATAGTAAGACAAAAAAATCCTTCTAAAGCAGTTTTTCAAGCTTTTAGAATAGCAGTGAATGATGAGCTAAATGCCTTAAGTGAAATGCTAAGCCAACTTCCTAATTTATTAAAAAAAGACGGAAAAATAGCGATTATAACATTTCATAGTAAGGAAGACGTTATAGTTAAAAAGTTTTATCAAAATTTAAATTATTTTGACCCTAAGTTAAAAAAAATACTAATTGTTGAAACTCATAAGTGA", "aa": "MEHIPVLLNEVIEQLSIKPDGIYVDLTLGRGGHSQEILKRLTTGKLICFDKDIQAINESREKLIKISQNFILIKSDFRNITCELKKLNIDKVDGILADLGVSSPQLDDPNRGFSYSINSDLDMRMDLDNTLTAKEIINNATEKEIIDILQSNADVKLSNLIAKAIVKNRPISDSFTLNQIIKDSLPAKIVRQKNPSKAVFQAFRIAVNDELNALSEMLSQLPNLLKKDGKIAIITFHSKEDVIVKKFYQNLNYFDPKLKKILIVETHK", "aa_hexdigest": "0184253a5e0f8c695f0b4111da5dcd67", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "ups": { "uniparc_id": "UPI000DED655C", "ncbi_nrp_id": "WP_114091198.1", "uniref100_id": "UniRef100_D1J816", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DED655C", "RefSeq:WP_114091198.1", "UniRef:UniRef100_D1J816" ] }, "ips": { "uniref100_id": "UniRef100_D1J816", "uniref90_id": "UniRef90_D1J816" }, "psc": { "uniref90_id": "UniRef90_D1J816", "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "LMJBGEANGM_562", "locus": "GCA013368015_00540" }, { "type": "cds", "sequence": "contig_1", "start": 307016, "stop": 307456, "strand": "-", "frame": 2, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "RefSeq:WP_012855507.1", "SO:0001217", "UniParc:UPI0001BC21EB", "UniRef:UniRef100_D1J817", "UniRef:UniRef50_Q6KHR3", "UniRef:UniRef90_D1J817" ], "nt": "ATGTTTGGTAAATATTATCGCCAAATTGATGATAAAAATAGAGTTGTTGTTCCGCCTAAATTACTACAAGAATTAGGCAAAGAATTCTATATCACAATTGGAATAGATAAAAACTTAGTATTAAGAACGGTTTCTGAGTTTCAAAAAATGAAAACTAAATTGGAAGAAGGAAACACACTTAACAAAAACGTAAGAGATTTCATTCGCTACATTTTTGGAAATACTGAAGTTGTATCTCCTGATAAAAATAACAGAATCATGATTCCCAAACATCTTTTAAATAAAGCCGCTATCGACAAAGAAGTTGTTTTCCTAGGAACTGGTGATACTTGTGAATTATTTGCTAAAGAAGTTTATGATGAACAAGAAAGTTACTATGAAAATGATGATAATGTAAATGACTTGGCTCAAAAATTATTTGAAGCAGGAGTTAAGTTCTAA", "aa": "MFGKYYRQIDDKNRVVVPPKLLQELGKEFYITIGIDKNLVLRTVSEFQKMKTKLEEGNTLNKNVRDFIRYIFGNTEVVSPDKNNRIMIPKHLLNKAAIDKEVVFLGTGDTCELFAKEVYDEQESYYENDDNVNDLAQKLFEAGVKF", "aa_hexdigest": "90a8ff3ff46d05845bdb9147a418c90f", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC21EB", "ncbi_nrp_id": "WP_012855507.1", "uniref100_id": "UniRef100_D1J817", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21EB", "RefSeq:WP_012855507.1", "UniRef:UniRef100_D1J817" ] }, "ips": { "uniref100_id": "UniRef100_D1J817", "uniref90_id": "UniRef90_D1J817" }, "psc": { "uniref90_id": "UniRef90_D1J817", "gene": "MraZ", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_Q6KHR3", "cog_id": "COG2001", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_Q6KHR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR3" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "LMJBGEANGM_563", "locus": "GCA013368015_00541" }, { "type": "tRNA", "sequence": "contig_1", "start": 307647, "stop": 307722, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 79.9, "nt": "GCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 307680, 307682 ], "id": "LMJBGEANGM_16", "locus": "GCA013368015_00542" }, { "type": "tRNA", "sequence": "contig_1", "start": 307813, "stop": 307887, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 77.6, "nt": "GCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 307845, 307847 ], "id": "LMJBGEANGM_17", "locus": "GCA013368015_00543" }, { "type": "tRNA", "sequence": "contig_1", "start": 307892, "stop": 307967, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.5, "nt": "GGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 307925, 307927 ], "id": "LMJBGEANGM_18", "locus": "GCA013368015_00544" }, { "type": "tRNA", "sequence": "contig_1", "start": 307970, "stop": 308045, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 82.1, "nt": "GGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 308003, 308005 ], "id": "LMJBGEANGM_19", "locus": "GCA013368015_00545" }, { "type": "cds", "sequence": "contig_1", "start": 308170, "stop": 309174, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE", "UniRef:UniRef90_UPI00280AD9DE" ], "nt": "TTGGTTATATTAATAGTTACCGTAACACTTGTATTAAAAGGAAATAATATTCATGTTTATGGGCTTTTTAATACAAATGCAGAACATGGATTTTTAATAAGTTATTTAACACTTATATATAGTTTTTTATCAACACTTCAATCAATGCTTACTATATTAGTTTATGCTAGAATTGGTATTGCATCTGCTGTTAGGGTTAATACGCTTTTAAGCCTACCAAACACACATATAAAAAACCACTCAAAGGGAATTGTGATAGAAAATCCTGTTGGCAATATAACATTTGATCATGTATATTTTAGATATAATGAAAATTCGCCTAAATATCAACTAACAGATGCTTCATTTAATGCAAAGGCAGGACAAACCATTGCTTTAGTTGGAGAAACTGGAGCAGGTAAAACTACTATTATAAATTTATTGAATCGTTTTTATGATTATGAAAAAGGTTCTATAAAGATAGATGGAATAGAGCTAAGAGAAATTTCAAAAGACAGTTTAAGAGATTTTACGGCAGTTGTTTTACAAGATAGCTTTATGTTTAATGAAAGTATTTTAAATAACCTTTTAGTAGGAAAGCCAAAAGCAACCAAAGAAGATGTAATAAGGGCAGCAAAATTAGCATCTGCTCATCATATTATTGAAAAGTTAGAACATGGATATGATACTATAATTGATAATAGCACTTCAATATTATCTAAAGGAGAAAAGCAACTTCTTTCAATAGCTAGAGCAATATTAGGAAACAAAAAGATCTTAATATTAGATGAAGCAACATCGAACGTAGATTCTAATACAGAAAAAATAATTCAACAAGCATTAAAAGAAAATATCATGGTTGGTAAAACATCAATAGTTATTGCCCACCGTCTAAGCACGATTAAAAATGCTGATTTAATCTTAGTTGTAGATGATGGTCGTATTATTGAACAAGGAACCCATAAGCAATTGCTAGAATTAAATAAAAAATATGCAAGGTTATATTACTCACAATTTGATGATTAA", "aa": "MVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPNTHIKNHSKGIVIENPVGNITFDHVYFRYNENSPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGKPKATKEDVIRAAKLASAHHIIEKLEHGYDTIIDNSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD", "aa_hexdigest": "cd0670d72bdf88c026267bf17c3f93d0", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 308170, "stop": 309474, "observations": { "mutation": [ 309198 ], "stop": [ 309198 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280AD9DE", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.988, "score": 625.0, "evalue": 1.52e-223, "gene_start": 1, "gene_end": 334, "reference_start": 147, "reference_end": 480, "reference_sequence": "MIIKNQVILFLFLVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINPWIVFTNNILVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPSTHIKNHSKGIVIENPVGNITFDHVYFRYNENSPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKEDVIRAAKLASAHHIIEKLEHGYDTIIDDSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD" }, "paralog": false, "identity": 0.9907834101382489, "score": 825.0, "evalue": 1.15e-304, "description": "Internal stop codon at 309198. Nonsense mutation around 309198.", "psc": { "uniref90_id": "UniRef90_UPI00280AD9DE", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280AD9DE", "UniRef:UniRef50_UPI00280AD9DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE" ], "product": "ABC transporter ATP-binding protein" } }, "id": "LMJBGEANGM_564", "locus": "GCA013368015_00546" }, { "type": "cds", "sequence": "contig_1", "start": 309196, "stop": 309897, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGTTTTGTTTTCATTTTTTCATGCAATTTTTTATGCAATAGGTTCATTTTTAATTGGATATATTTTTACTATATGTTTAACAAAAGATGTATTGGATCATAAAACACCTTTTAATTTAAAATTATTTATAGGTATGGCTTCATGTTTGGTTATATCGTTTATATTGTATGGAATTTTTAGATATGTTTCAGGAGTATTCTTTGTAAAAATAACTTTTTCAGTTTGCGAGAATATAAGAAAAACCGCCATGAAAAACCTTTTATATATTCCCGTTTCATATTTTGATAATCAAAAATCAGGTAATATTATTTCTATTTTAGTAAATGATATTAATAGTTTATCTGAAACTATATTTAAACTATTAAATGAATTAATCCAATGTGCTATAAATGTTATTTTATCTGTAATATTTTTATCAATGGTTAGTCTGGTTTTATCTGCTGTTGTTTTACCTGTTCTATTCTTATTTTCATTTATAGGAATATTAATTACTAAAAAAGCTCAAAAAGCATTTATTCAAACCAGAAAACATTTTGGGGAATTAAATGCTTTTGTCGAAGAAATGCTAACTAATTCTAAAATTATTAAAACTTTTGAACAACAAGAACAAGTTAATAATCATGGTAAACAAATAGCAAGAAACATATTTAACACAGCATTTACTGGGGATCTTAATAACCAAATAATTAACCCTTGA", "aa": "MAVLFSFFHAIFYAIGSFLIGYIFTICLTKDVLDHKTPFNLKLFIGMASCLVISFILYGIFRYVSGVFFVKITFSVCENIRKTAMKNLLYIPVSYFDNQKSGNIISILVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINP", "aa_hexdigest": "511f8b17b57075d707311b7b34376e9d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26396.00490000002, "isoelectric_point": 9.158330345153807 }, "id": "LMJBGEANGM_565", "locus": "GCA013368015_00547" }, { "type": "cds", "sequence": "contig_1", "start": 309898, "stop": 309993, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACTAAAGAAAAGCAAAAACTTTCATTTATTACTTTGTTTAAATTAGTTTTTAAATATTCAGGTGAATCAAAAACAAAATTTTGA", "aa": "MKKRTKEKQKLSFITLFKLVFKYSGESKTKF", "aa_hexdigest": "9e033a066f30cb864e5c512b3f8fafc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3770.5296000000008, "isoelectric_point": 10.363437843322753 }, "id": "LMJBGEANGM_566", "locus": "GCA013368015_00548" }, { "type": "cds", "sequence": "contig_1", "start": 310008, "stop": 310976, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "nt": "GTGGTGCTTTATTCATTAGTTGTTCATGATGTTATTTCTAATGTAGCAACATATGATACAATTGTAAGATTTAATATTTTTATTGACTATTCATTTTATGTAACATTTGGTGTTATATTGGGGGCAGTATTTATTTCAGCATTTGTAAAAGGAAAAATTTCTGCTACTAGAGTTATAGAAGTATTAAATACTAATGAAGATAAAATTTATGTATCAAATGGCAAAAAAATAACAAAAAACTTCGATTTAAAAGTTGAAAATTTAAGCTTTAAATATTATTCTTCAAATCCAAATTATACTTTACACAATATAAGTTTTAGCCTACCGTTCGGAAAAACTCTTGGATTAGTTGGCCCTACTGGAAGTGGTAAAAGCATACTTGCGAATTTATTGGTTAATAACTATCTATATAATGAAGGATCGATTTTAATTGGGGATAATGAAATTAAAGAAGTTAATACAAAAAATTTACATGAAACAATCGGAATTGTTTACCAAGATGCTCTATTAAATACAGGAACAATAAAAGAAAATATGCTTTTTGCTAAAAATGATGCAACAGATGAAGAAATAAATCAAGCATTACGTGACGCTTGTGCATATGATTTTGTTTATAAATTTGAAGAAAATTTAGATCATGTCATTACTCAAGGGGCAACAAACCTTTCAGGTGGCCAAAAACAACGTCTTTCAATTGCAAGAACTTTATTAACTAAACCAAAAATTTTAATATTAGATAACTCAACTAGTGCTTTAGATAATATTACTGCACACCAAGTTTTACAAAATATAAAAAATAAATATTCATGTTCAACTATAATAATTAGCCAAAAATTGCCATTAATAAAAAATGCTGATGAAATTATTGTTTTATCTGATGGAAACATGTTGGATAAAGGAAATCATGAAGAATTAATGAAATCATGTTCATATTATAATGAATCATATAATAACCAATTAAGCCAATAG", "aa": "MVLYSLVVHDVISNVATYDTIVRFNIFIDYSFYVTFGVILGAVFISAFVKGKISATRVIEVLNTNEDKIYVSNGKKITKNFDLKVENLSFKYYSSNPNYTLHNISFSLPFGKTLGLVGPTGSGKSILANLLVNNYLYNEGSILIGDNEIKEVNTKNLHETIGIVYQDALLNTGTIKENMLFAKNDATDEEINQALRDACAYDFVYKFEENLDHVITQGATNLSGGQKQRLSIARTLLTKPKILILDNSTSALDNITAHQVLQNIKNKYSCSTIIISQKLPLIKNADEIIVLSDGNMLDKGNHEELMKSCSYYNESYNNQLSQ", "aa_hexdigest": "42a94e5b35c1b29f24fb586876b7b8e3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DFCB5D", "query_cov": 0.9440993788819876, "subject_cov": 0.9589905362776026, "identity": 0.52, "score": 315.0, "evalue": 1.35e-103, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DFCB5D" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFCB5D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "LMJBGEANGM_567", "locus": "GCA013368015_00549" }, { "type": "cds", "sequence": "contig_1", "start": 311055, "stop": 311312, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTGGTGGATGTATTTTAGCCGTCATGAATAACGTAAAAATGGCAATAGGATTAATTTTGTTAGTTCCGATAATTTTAACAATAGTTTCTATTATGGCCAAAAAAGCAAGTCCTTCAATTAAAAAAGCTCAATTAGCCGTTGAAGAAATAACTAAGCAAGTTGATGAAAATATATTAGGATCTCGTTTAATAAAAATATATGATTTATATAAAAATAGAGAAGAAAAGTTTGCAAAAATAAATAAAACATGA", "aa": "MIIGGCILAVMNNVKMAIGLILLVPIILTIVSIMAKKASPSIKKAQLAVEEITKQVDENILGSRLIKIYDLYKNREEKFAKINKT", "aa_hexdigest": "f4b0a80b858dff6d1eaa5909d85d28fe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9426.415499999992, "isoelectric_point": 9.673819541931152 }, "id": "LMJBGEANGM_568", "locus": "GCA013368015_00550" }, { "type": "cds", "sequence": "contig_1", "start": 311358, "stop": 311750, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGGAAGTTTTATTTCTTTAATTTCAGTTATATTTGAATTGATTACTCCTACATTGATTTCTCAATTCATTAGATTGCTTTTTATTGAAAATAAAAATAACCAAGTAGATTTAAGTTTTTTTAATGGATTAATAAACTTTACATTTAATAATGCTATTAATGCTCGTAATTATATGATTTATTCTATAATAGTTTTAACTATAATTTCAATGTCTTTAACATTTGGCTGCACTAGCTTAATTGTATATGCAGCAGAAAGAAGTAGTAGATATATTAGAGAAAACTTTATTAGAAAATTAAATACATTAAGTTTAAAAAATATATCTGATTTGAAACCTGAATCATTAATTACTAGGGTGAGTGATGATATTGCGGTTTTCTGA", "aa": "MFIVGSFISLISVIFELITPTLISQFIRLLFIENKNNQVDLSFFNGLINFTFNNAINARNYMIYSIIVLTIISMSLTFGCTSLIVYAAERSSRYIRENFIRKLNTLSLKNISDLKPESLITRVSDDIAVF", "aa_hexdigest": "a0f70e5f1e0a78d1ef6d00af54300b82", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14874.277999999978, "isoelectric_point": 8.88685398101807 }, "id": "LMJBGEANGM_569", "locus": "GCA013368015_00551" }, { "type": "cds", "sequence": "contig_1", "start": 311979, "stop": 312794, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATCTTACTGTTTCATTTAAAGATTATGAAAATAGTACTAAGAAATCAAAATTTGTTGAAATTGATTTAAGTAAATTGGGTGTTGATGAATATAACAAGATTAGAAAACAAAATAATGAAACTGAAATAGATGACCAACAAGCCCCAACATCAACTATTGAAGACCAAGACGTAAAAGAATTAAACAAAGAAGCATTCTTGAAATATTTCAAAGAAAACAAAGATAAATTATCTAAAGATGAAGTTAAAAATAATTCAACATTTATGTTTATAAAAAAATTGAAAAATATTAGTCTTTTAAGCAAAGACATTGAAAAAGAAATAATTGAAAAAGGTGATAATTACATTTCGGTTCAAAATTTTGATTATGATAAAACAACATTAAAATTGAAACATTCAATGTTTGCGTTTCAATATACAAATAGTATGGTTAATGTACAAAATGTTTATATCTTTTCATTTCCTAAAATAGAAAATAACAAAGTAAAATCAATTAGAGTTTCTGTAGTTTCTATTCAAGATTTTCAAAATAATGATTTTAGCAATATGTCATCTAAACGTGTCAATGTTAATGAAGTGGTAAATGAAGATAAAAACTTTATGCAAAAAATAGTTTTAAAAGAACATTTTAGAGAATTAATAACTGTAAAATACATTGGCGATAAAAAAACAGTTGATGAAGCAATTAAAGATGATATTTCTAAATTCGAATACAAATTACCAGAAGGATTAACATTTGAAAAAATCTCAGTTAAAAAATATACAAACAAAGGTAAAGAATATATAAAATATACAATCACTGCTAAATGA", "aa": "MINLTVSFKDYENSTKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKEAFLKYFKENKDKLSKDEVKNNSTFMFIKKLKNISLLSKDIEKEIIEKGDNYISVQNFDYDKTTLKLKHSMFAFQYTNSMVNVQNVYIFSFPKIENNKVKSIRVSVVSIQDFQNNDFSNMSSKRVNVNEVVNEDKNFMQKIVLKEHFRELITVKYIGDKKTVDEAIKDDISKFEYKLPEGLTFEKISVKKYTNKGKEYIKYTITAK", "aa_hexdigest": "d4c9eec30714f23e1b1a73843c549101", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31867.9671, "isoelectric_point": 8.887627601623539 }, "id": "LMJBGEANGM_570", "locus": "GCA013368015_00552" }, { "type": "cds", "sequence": "contig_1", "start": 312861, "stop": 313781, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821", "UniRef:UniRef90_D1J821" ], "nt": "TTGGTAAATCCTGAAAAACCTCTTAGAAGTGGAGACAAGTTAAACTCTAAATTGTTATTAACAAAAGATGAAAAAGGAAACTTTATTTTGAAATATAAAGTTGCAATTTTTAAACAAAATGGTAAACATATTATTTCAAACAAAGTTTATGAAAGTAATTTAGGAAACTTAGATTCTGGATTAGATCAAGTTTCAGAAGAAGATTTTAAAGAAGTTTCAAAAAATCTAGTCTTTGACTATCCTAATAAACAAGAAACATACATAAAAGACGCAGACACATCAAAAGTATTTTTAAAAACTATTAAAGAAAACTTTTCAATTGTTAAAGATTCGTTAAAAATTACTAAAAATACAGGAGAAGATGGAACATATGACTTGACAATTCTTTACTATTTATCATACAAGAATAAACAAATCTCAAGTCAAAAATATATTTATGAAATCAAAGGTTTTAAATTAACTCCAGAATTGCAAGAAAAAATAGATAAGGCAAGAAAAGAACTAAAAGATCAAATTGAAAAAACAAATGTTTTAGTTGCAAATGCCAAGTCATATCAAGATATTTTAAAAAATAAACAAATAAATAATTTTGATAAAAAACCAAATTTTGCAGTTCAAGGTTATGATCAAGCTTCATATTCAGTAGTATTAAGCAATTTAAAAGTTGAAGAAGATAAAAAAATAACATTGACTTTAACCTTGTCAAGTCTTTTAAATAAAGAAATAAGTGAATCTAAAACTATTGAAGTAAAATTAGATAAATTTGCAAATGGTTTAGATAATATTGCATTGCTATCACCAGAAAAACAAGATGAACTTTTAAACAAAGCTTTAGAAACTTCAATTTATCCATTTTATACTAAAGATAAATATTACATTTCAAAGAAATCTATTGACAAATTAGAAAATAAATCATACTGA", "aa": "MVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLTILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKIDKARKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVVLSNLKVEEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSPEKQDELLNKALETSIYPFYTKDKYYISKKSIDKLENKSY", "aa_hexdigest": "5356151eb19425b60b5d8221a57888c7", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 312558, "stop": 314075, "observations": { "mutation": [ 312863, 313811, 313955 ], "stop": [ 312863, 313811, 313955 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J821", "query_cov": 1.0, "subject_cov": 0.523972602739726, "identity": 0.98, "score": 559.0, "evalue": 3.77e-196, "gene_start": 1, "gene_end": 306, "reference_start": 142, "reference_end": 446, "reference_sequence": "MKKHTKILLALGSVATSVFAIPLVAAGCIKTKKPEVKPEEPKKPEGEKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKITWNAENLEFIKKVLNNLDKYKILFERNNKNIMAFEGAYDFKNQEFAIAKWNSKSGVSVQLVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKIDKARKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVALSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSFEKQDELLNKALETSIYPFYSKDKYYISKKSIDKLENKSYWINNKNSEINYTLGELKFEGDKCLINLTVSFKDYENSTKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKDAFLKYFKENKDKLSNDEVKNISTFMFVEKLKNISLLSKDIEKRNDWKRW" }, "paralog": false, "identity": 0.9565217391304348, "score": 934.0, "evalue": 0.0, "description": "Internal stop codon at 312863, 313811, 313955. Nonsense mutation around 312863, 313811, 313955.", "psc": { "uniref90_id": "UniRef90_D1J821", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J821", "UniRef:UniRef50_D1J821" ] }, "pscc": { "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821" ], "product": "Variable surface lipoprotein" } }, "id": "LMJBGEANGM_571", "locus": "GCA013368015_00553" }, { "type": "cds", "sequence": "contig_1", "start": 313953, "stop": 314231, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATACAAAAATTTTATTAGCCTTAGGTAGTGTTGCAACCTCGGTTTTTGCAATACCATTAGTGGCAGCAGGTTGCGTAAAAACAAAAAAACCAGAAGTTAAACCAGAAGATAAACCAGGAACAACACCTGAAGTTAAACCAGAAGATAAACCAGAAGATAAACCAGGAACAACACCTGAAGTTAAACCGGAAGATCCAAGTCATGAAGAAGAAACAAATAAAAATGATGCTGATAAATATGGCGAAGAATCAATTTCAAAAATAACATGA", "aa": "MKKHTKILLALGSVATSVFAIPLVAAGCVKTKKPEVKPEDKPGTTPEVKPEDKPEDKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKIT", "aa_hexdigest": "aa4032334744103de3473b9257b88a8c", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9952.152399999997, "isoelectric_point": 5.410128593444824 }, "id": "LMJBGEANGM_572", "locus": "GCA013368015_00554" }, { "type": "tRNA", "sequence": "contig_1", "start": 314509, "stop": 314584, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.9, "nt": "GCATCCTTAGCTCAGTTGGCAGAGCAAGGGACTCTTAATCCCTGGGTCGTGGGTTCGAGCCCCACAGGATGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 314577, 314579 ], "id": "LMJBGEANGM_30", "locus": "GCA013368015_00555" }, { "type": "tRNA", "sequence": "contig_1", "start": 314685, "stop": 314769, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 60.8, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGTTAGTTTTAGGCACTAATGCTTTGCGGCGTGAGGGTTCAAGTCCCTCCTCGCGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 314739, 314741 ], "id": "LMJBGEANGM_29", "locus": "GCA013368015_00556" }, { "type": "tRNA", "sequence": "contig_1", "start": 314773, "stop": 314848, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 88.2, "nt": "GCCGTTTTAGCTCAGTTGGCAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCAAGTCCTATAAACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 314813, 314815 ], "id": "LMJBGEANGM_28", "locus": "GCA013368015_00557" }, { "type": "cds", "sequence": "contig_1", "start": 315058, "stop": 315846, "strand": "+", "frame": 1, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "db_xrefs": [ "COG:COG0130", "COG:J", "EC:5.4.99.25", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "KEGG:K03177", "SO:0001217", "UniRef:UniRef50_A0A501X9B0", "UniRef:UniRef90_D1J822" ], "nt": "ATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAGTTGGACATTGTGGAACATTAGATCCCGAGGCTGAAGGTCTTTTAATCGTAGCAACAGATGAAGATACAAAAGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCAAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTGCCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATCGAATTTAATAAAGTTAATCAATCCATCATCATTAAGGCCAATGTTTCAAAAGGCACATATATTAGATCATTGATTCATGATTTAGCTAATATATGTAACACAGATGCAGTAGTAGTTGTTTTAAAGCGAATAAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTCAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATAACATAGTAGGATGA", "aa": "MFYKLYKKRGQSSFFAIKKFAKNNNINKVGHCGTLDPEAEGLLIVATDEDTKALSLIENQNKEYYVEAKFHYSSDSFDLGTYVTKIQNFQIITKSQIIQALEILKKQTKQVPPIYSAKKINGTRAYKLARDNKEFSLKANDIKINNLELIEFNKVNQSIIIKANVSKGTYIRSLIHDLANICNTDAVVVVLKRIKIGNICLNGQEEFEKITNLNELFNVKLFKLTKNDIQILANQNSNSCYFESLKNFCGNIIFIYLNNIVG", "aa_hexdigest": "9a3e4efe051a77b1c0156df7fb704737", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J822", "query_cov": 1.0, "subject_cov": 0.9128919860627178, "identity": 0.9890000000000001, "score": 502.0, "evalue": 4.08e-179, "valid": true, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "ec_ids": [ "5.4.99.25" ], "uniref50_id": "UniRef50_A0A501X9B0", "cog_id": "COG0130", "cog_category": "J", "kegg_orthology_id": "K03177", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9B0" ], "product": "tRNA pseudouridine synthase B" }, "genes": [ "truB" ], "id": "LMJBGEANGM_573", "locus": "GCA013368015_00558" }, { "type": "cds", "sequence": "contig_1", "start": 315930, "stop": 316301, "strand": "+", "frame": 3, "gene": "cof", "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_D1J823", "UniRef:UniRef90_D1J823" ], "nt": "ATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTGTTGCCGTTCGGAGTTACTCAATTAACACCAAGAATGCAAGAAATGTTTGAGAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATTGATAACCCTTATGTTGATTATTTTATAGGTGCAAATGGAACTTTTATTCTTGATTTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTGTTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGA", "aa": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFIGANGTFILDLRTKEYIFEKTINYKDFVEFRKYASEHKLSFSFVGNKWGYYNELFNIDH", "aa_hexdigest": "1d96cd0a73be2f35c123cce0dec97131", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 315930, "stop": 316604, "observations": { "mutation": [ 316299, 316452, 316467 ], "stop": [ 316299, 316452, 316467 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J823", "query_cov": 1.0, "subject_cov": 0.4606741573033708, "identity": 0.9840000000000001, "score": 256.0, "evalue": 9.87e-85, "gene_start": 1, "gene_end": 123, "reference_start": 1, "reference_end": 123, "reference_sequence": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFVGANGTFILDLRTKEYIFEKTINYKDFIEFRKYASEHKLSFSFVGNKWGYYNELFNIDHWFYRPYKDKFIPEKEFESQHDKNYLITISTPTPSIVAADLEKFFKEKNLDLWILAQWNGGIFISAKGITKAVGLKILGDLLNISLDEMVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAAKEICLPVTEDGPYWKLRDKGFFD" }, "paralog": false, "identity": 0.9776785714285714, "score": 443.0, "evalue": 8.77e-161, "description": "Internal stop codon at 316299, 316452, 316467. Nonsense mutation around 316299, 316452, 316467.", "psc": { "uniref90_id": "UniRef90_D1J823", "gene": "Cof", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_D1J823", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J823", "UniRef:UniRef50_D1J823", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J823" ], "product": "Cof-type HAD-IIB family hydrolase" } }, "id": "LMJBGEANGM_574", "locus": "GCA013368015_00559" }, { "type": "cds", "sequence": "contig_1", "start": 316560, "stop": 316703, "strand": "+", "frame": 3, "gene": null, "product": "Hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "nt": "ATGGTAGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTAGGATTAGGTATTGCTATGGGCAATGGAGAAGATGTTACTAAAGATGCTGCAAATGAAATTTGCTTGCCAGTTACTGAAGATGGTCCATATTGA", "aa": "MVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAANEICLPVTEDGPY", "aa_hexdigest": "574b0db1bae9dc23f0128d424f25fbcc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W0C783", "query_cov": 0.9361702127659575, "subject_cov": 0.8, "identity": 0.568, "score": 53.5, "evalue": 1.64e-08, "valid": false, "product": "Hydrolase", "uniref50_id": "UniRef50_A0A2W0C783" }, "pscc": { "uniref50_id": "UniRef50_A0A2W0C783", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "product": "Hydrolase" }, "genes": [], "id": "LMJBGEANGM_575", "locus": "GCA013368015_00560" }, { "type": "cds", "sequence": "contig_1", "start": 316748, "stop": 317527, "strand": "+", "frame": 2, "gene": "spoU", "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "COG:COG0566", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9", "UniRef:UniRef90_D1J824" ], "nt": "ATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTCTAAAATATAAAAAATTACTTACTCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGTTATCATTTAGTAAAAGAAGCAATCAAGGCAAATATAGCTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAAACATTATCAACAACGCAAACACCTCAAAATATCATTGCAATTTGTAAAATACAAAATTTAAATATTAAAGCAAATAAAATAATAGCCCTTAATAAATTACAAGATCCCGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGCTTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATGACATTGATTTTAGAAATAAAAAAATTATTGTTGTTTTGGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTATATTCCTATTGAATTTGAAAGCCTCAATGTTGCTTGCTGCGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAA", "aa": "MVDFYLFYFKILILGKKHMAITSTQDPKILKYKKLLTPKGRQEQQLFIVEGYHLVKEAIKANIAIDILEFNDILTYKNSTQVTRAIIKTLSTTQTPQNIIAICKIQNLNIKANKIIALNKLQDPGNIGTIFRLAKSFGFDSVIVENIDIYNEKIIRSSQGAMFSLNIIKTNDLSKELSAFKKDDFLVYSTTLNKNSIKLNDIDFRNKKIIVVLGNEGNGIDKKIIDLSDFNVYIPIEFESLNVACCGAIIMNKIYNGDK", "aa_hexdigest": "de8d6cc9cbc239ce0659d84576efe20e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J824", "query_cov": 0.9305019305019305, "subject_cov": 1.0, "identity": 0.971, "score": 456.0, "evalue": 9.27e-162, "valid": true, "gene": "SpoU", "product": "tRNA/rRNA methyltransferase", "uniref50_id": "UniRef50_A0A3B0PPX9", "cog_id": "COG0566", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PPX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [ "spoU" ], "id": "LMJBGEANGM_576", "locus": "GCA013368015_00561" }, { "type": "cds", "sequence": "contig_1", "start": 317686, "stop": 318159, "strand": "+", "frame": 1, "gene": "ribF", "product": "FAD synthase", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6", "UniRef:UniRef90_D1J825" ], "nt": "ATGATGTTTAAAAATCCAGTTAAAAATTCTCGGGTATTAGCCAAAAAGGCAATGCAACCAAAAACCAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGCGATTTTATTCAAGGATTAAAAACAAACAACGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGATTTGGTTTCAATAGAGAGGGAAACATTGATAGATTAAAAAAATATTTCAATGTATATGTTGCTAATGAATTAAAGGTAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAGTGGCTTGAATAAATTACTAATAGACAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTCAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGA", "aa": "MMFKNPVKNSRVLAKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKTNNVKNVVCGQDFRFGFNREGNIDRLKKYFNVYVANELKVNKQKISSTIINEFIEEGNISGLNKLLIDKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVV", "aa_hexdigest": "364e2555b53294f972b06d591b099f04", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "both", "pseudogene": { "start": 317527, "stop": 318399, "observations": { "mutation": [ 317542, 318157 ], "stop": [ 317542, 318157 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J825", "query_cov": 1.0, "subject_cov": 0.5358361774744027, "identity": 0.9620000000000001, "score": 302.0, "evalue": 7.6e-102, "gene_start": 1, "gene_end": 157, "reference_start": 54, "reference_end": 210, "reference_sequence": "MQVFDWDFKAKLNINEPLTICLGSFETLHLGHNELFKIAKDLKQSYPDQKLAIMMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKVNKQKISSTIINEFIEEGNINGLNKLLIGKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVVWNNIEYHGLIFINKNQFDSNNNLQENNIIYLFDIDYFISKYQELYIEFLQCIRHINNNSENQISTNDIEIGKKYFISKEKMNS" }, "paralog": false, "identity": 0.9482758620689655, "score": 555.0, "evalue": 8.37e-204, "description": "Internal stop codon at 317542, 318157. Nonsense mutation around 317542, 318157.", "psc": { "uniref90_id": "UniRef90_D1J825", "gene": "RibF", "product": "FAD synthase", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_A0A448ZYJ6", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J825", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_A0A448ZYJ6", "COG:COG0196", "COG:H", "KEGG:K11753" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6" ], "product": "FAD synthase" } }, "id": "LMJBGEANGM_577", "locus": "GCA013368015_00562" }, { "type": "cds", "sequence": "contig_1", "start": 318464, "stop": 318976, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATGGCTATGAAATTGTTAAACAAATGTTTAATTATGGCCATACTTATAGAGAAAAAGAAATCAATAAAATGATAAAAGATATATTCATGAACCTACCTTTAAATAAAAACGAATATACTTATAAATACCTTTGTGAAAATTCTATAAATTATTTTGTTGACAATAAAATGTTAGAAAAATTAATAAATCCTGATGGTGAAATCTTTTATCATGATGTTTCAAAGCCATATACTTTTAGAATGCCAAAAGATTGCGACAATAGAAAAATATCAGAAATAAGTTCATTGGAATTCTTTGATTTAATAAAACAAGTTTTAAACAATTCAAAAAGTTCTATTTTAAAAAATAATTTAACTAATACAATTTTAAAAATCTTACTACAAGCAAGAACAGAAAATAATATTTCAAAATTAAACGATCTATATGGATATTTAAATAATTTAGGGTTAATTAAAGTTATTTCAAACGGTGATATTGAATATATTGACTTGAACAACAAAGATTAA", "aa": "MLNGYEIVKQMFNYGHTYREKEINKMIKDIFMNLPLNKNEYTYKYLCENSINYFVDNKMLEKLINPDGEIFYHDVSKPYTFRMPKDCDNRKISEISSLEFFDLIKQVLNNSKSSILKNNLTNTILKILLQARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD", "aa_hexdigest": "7c059745e9bdbdfa79d6e75e0af4d24b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20089.847000000016, "isoelectric_point": 6.739251518249512 }, "id": "LMJBGEANGM_578", "locus": "GCA013368015_00563" }, { "type": "cds", "sequence": "contig_1", "start": 319229, "stop": 320101, "strand": "-", "frame": 2, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6", "UniRef:UniRef90_UPI001F43A718" ], "nt": "TTGCCTTCAAATAAGTTTGTTTATGATAATAAATTAATTACTTTTCCAACATATCAAAAAACTGCCAAGAAAGACGGGCAATTGCTTCCTTTTGAAGGCGTTTCATTTATTAAGGTCAATGGAATTTATGAAGATCAAACAAACGAAATTGAAGCAAATAGAATCATTGAATTAATAAAGCAATTAAAAGATTATTATGGCAATACAAAAACATTTGGGGTTGTAACGTTTAATTTAAAACAAGCTAACTTAATTGATCACAAATTATTCTTATTTATGAATGAAAATCCTGAATATATGGATTTAGACACAGAAAATAAAATTTTTGTTAAGAACATTGAAAGTGTTCAAGGTGATGAAAGAGACATCATTATTTTAGGAATTACAAATAGTTGGGATAAAAACGGAAGATTGTCAAGATTCTTTGGACCATTAAATAATGTTGGCGGTGAAAAGAGACTAAATGTCGCAATAAGTAGAGCCAAGGAAGCAACTATTGTTGTATCTTCAATATCATATTCTGATTTAAAAGTAGACGATTTAAAAAATGAAGGACTAAGATTTTTAAAGCAATATTTACAATTTGCAGAATTTGGTTACGACAGCAAATCAGATGATATATTACAAGATGACACTAAAACATTTGATAGTGAGTTTGAAATCCAAGTTTACGATGAACTTAAGAAGCTTGGATATAAAGTAAATACTCAAGTAGGATGTTCGAAATTTAAAATAGATTTAGCAATTGTTGATGATAAAAATCCATCGAAATATATTTTAGGTATTGAATGCGATGGCGCCACATATCATTCATCAAAAAGCGCTCGTGATAGAGATGCGATTAGACAAATGCTACTTGAGTCAAGAGGTTGA", "aa": "MPSNKFVYDNKLITFPTYQKTAKKDGQLLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGYKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRG", "aa_hexdigest": "32475149eef5eb460187fad1c8c07fcd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 318926, "stop": 320074, "observations": { "mutation": [ 319201, 319213, 319231 ], "stop": [ 319201, 319213, 319231 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F43A718", "query_cov": 0.9689655172413794, "subject_cov": 0.5072202166064982, "identity": 0.996, "score": 556.0, "evalue": 1.17e-195, "gene_start": 10, "gene_end": 290, "reference_start": 19, "reference_end": 299, "reference_sequence": "MIEKSLYDFATPDFQTLPNKLITFPTYQKTAKKDGQLLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGFKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRGWIIHRIWSLNWFKNKRRELEKIVDKVNQIKQKKFVVKQNEYKTIENSQFEVIEKRDNFNFEKWLPFNYSYRKTLNFIEKSNKRNPLLNGYEIVKQMFNYGHTYREKEINKMIKDIFMNLPLNKNEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKTLLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD" }, "paralog": false, "identity": 0.9685863874345549, "score": 736.0, "evalue": 4.54e-269, "description": "Internal stop codon at 319201, 319213, 319231. Nonsense mutation around 319201, 319213, 319231.", "psc": { "uniref90_id": "UniRef90_UPI001F43A718", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F43A718", "UniRef:UniRef50_UPI0002FAB5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6" ], "product": "AAA domain-containing protein" } }, "id": "LMJBGEANGM_579", "locus": "GCA013368015_00564" }, { "type": "cds", "sequence": "contig_1", "start": 320132, "stop": 320569, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGCCTACGTTAAATTCGACAATATACAATGATGAATTAGAAATTTTAAAGAAGGAATATTCAAAAGTTAGAAATAAAAAACCGATTAGAAAACTATTTAAAGAAATTCCAAATTTAATTCAAAATATTAAGCCTTGTTTTATGATGTCTCCTTCTTCGCTAAGTACATATTTAGCGGATAGTAGCATTACATTTGATAGTGTAATTTTTGATGAGGCTTCTCAATTAACGACAGAAAATGCCTTGATATCTATGATTAAGGCTAAACAAATTATTATTGCCGGTGATATAAATCAATTGCCACCAACTAATTTCTTTAAAACTATGTCTAGCGAGCAAGAACCAAACGAAGATGAAGAAATAGTCAATGAAGAAAATGATTATGATTCACTTCTTGAAAGAATGCAAAGTTCATTTAGACAAGTTACATTAAAATGA", "aa": "MPTLNSTIYNDELEILKKEYSKVRNKKPIRKLFKEIPNLIQNIKPCFMMSPSSLSTYLADSSITFDSVIFDEASQLTTENALISMIKAKQIIIAGDINQLPPTNFFKTMSSEQEPNEDEEIVNEENDYDSLLERMQSSFRQVTLK", "aa_hexdigest": "ec16b0df06247633108957fce7a498fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 52, "aa_cov": 0.35172413793103446, "hmm_cov": 0.9807692307692307, "evalue": 9.329357099148935e-12, "score": 40.750946044921875, "start": 54, "stop": 104 } ], "seq_stats": { "molecular_weight": 16691.853299999988, "isoelectric_point": 4.7559118270874015 }, "id": "LMJBGEANGM_580", "locus": "GCA013368015_00565" }, { "type": "cds", "sequence": "contig_1", "start": 320672, "stop": 321370, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATCTCGAAATAATATTTTTAAGCGTATTTTTTCAAAGAGATATAGAGCAATAAAAAAAGAATTAAAAATTAATTTTATATCTTCTAATTTAAATTACAAAAACTTGTTAAAAGATTTAGTAGCATTAAATAACATTCAACTTGCTAAAAATGCAATTACGCTTTTAACAAATAAAATTCCTTTTTCATTTAAAGAAAACTATAATGAAATGGATCTTAATAGTTTAAAGCAACAACTAAATATATTTTCTAAATACGAAATTATTAATAATCAATTAAGTGAATTAAAATTTGACCAAGATTTAAAAATTAGCTTTTTTAACTTTTTATTAAGCCATATTCAATTAACAAATAATTACATAAATTCATATAAAAATTTAATGACTTTTGAAGATAAATTCAATGACTACTTTAATAATAAATATGTAGATTATTCAAATTCAAATTATAAAGTTAATGATTTTATAAAAAAACTAAATTTTTTAATTGATTCTGATGAATATCCTGAAGCATATTGTAGTTATATAAAATCAAGACAAGAATTAAAAAACAATCCCTTTACTAATGATTTTGTGGATGCTATTGAATTGCTAGATATTGATTGTTTAAAACAAAATAGTATTGAAAATATTTTTAATAAAAGATTTTATCAACTTTTAATTGATTACTATTTATCAATTGATTTAGCAGATTGA", "aa": "MKSRNNIFKRIFSKRYRAIKKELKINFISSNLNYKNLLKDLVALNNIQLAKNAITLLTNKIPFSFKENYNEMDLNSLKQQLNIFSKYEIINNQLSELKFDQDLKISFFNFLLSHIQLTNNYINSYKNLMTFEDKFNDYFNNKYVDYSNSNYKVNDFIKKLNFLIDSDEYPEAYCSYIKSRQELKNNPFTNDFVDAIELLDIDCLKQNSIENIFNKRFYQLLIDYYLSIDLAD", "aa_hexdigest": "0515fea4ea2bff329d48786c00557f0e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27855.435500000036, "isoelectric_point": 8.574117851257324 }, "id": "LMJBGEANGM_581", "locus": "GCA013368015_00566" }, { "type": "cds", "sequence": "contig_1", "start": 321431, "stop": 321727, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTAAAGCTTCAAGATTTAGAAAACATTAAAGAGATTTCTAGCCATTTAATAAATGAAATTCAAAGCCTAATCAATTTCTTAGATCTTGAAATTCCAAACAATTATCAACCATATATTAAAAATAGCAATTATTCATTATTTAATTCATTTTTTAAAATTTTTTCAAATATACAAGATATAGATATAAATCCAAAATATTTAGAAATTTTTTCGTATAACAAAACTAATGCAACACAAACCCTAAATGAAATACTATTGCAAAAAGAAAGAATTCAACCTGATATTGACTGA", "aa": "MDVKLQDLENIKEISSHLINEIQSLINFLDLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYNKTNATQTLNEILLQKERIQPDID", "aa_hexdigest": "49ae8030f93c02d9c3202f278d0fedc3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11628.996599999984, "isoelectric_point": 4.582098197937013 }, "id": "LMJBGEANGM_582", "locus": "GCA013368015_00567" }, { "type": "cds", "sequence": "contig_1", "start": 321743, "stop": 322498, "strand": "-", "frame": 2, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65", "UniRef:UniRef90_UPI0024114E65" ], "nt": "ATGGACATTTATTTAGATGTTGATAAAAACAAAGATAATATCATTAATTCAGAAATTATGAATGCAATGACAGGGATTAACAATGTTTTTAGTTCTGAATATTTGTATTCTGAAGAAGAAGTTGATAATATAGTTTCTCCTAAAGGCTATTATCACTGCCTAATGACTAATTCTTCGCAAGAAGCAGCTATTCAATCAGCAATTAAAGGAAACAGTTTTATTATTCAAGGTCCTCCAGGAACTGGAAAATCTCAAACTATAGCAAATATAATAAGTGAATTAATTGCAAGAAATAAAAAGGTTCTATTTGTTGCTGAAAAAAAGGCTGCTTTGGATGTTGTTTATAAATCAATCCAAAATTTAGGATTTGCTAATTTTATTTTGCTTATTCATAGCAATAACAGCAATAAAAAAGATTTTTCTAATGATTTGTATGAAACATTGCAAGACGGTCAAAAATTTAAAAAAGTAAGTGACGAAGTTATTGCTAATAATGATTATTTATATACTGATTCATTAGAAAAAATGAATGATTTTGTTAGAAAGATATTATCAAATAGAAAACCACTAGGTCGCTCATTATATTTAATGATTGGTGATTATCAAAAATTGCTTGCCAATACAAAAGGTATAAATTTTGATATTAAAGATTTTGAAAAAATTGATAATGAACAGTTTGTTGAAATTCAAAGTAGTTTAAATAAGTTTAATTTAAACTATAAAAATATAAATTTTAACGCTAAGGAAAGTTTATGA", "aa": "MDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKVLFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYTDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIKDFEKIDNEQFVEIQSSLNKFNLNYKNINFNAKESL", "aa_hexdigest": "d1e3197290deff253c7b3a5d769c2597", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 321440, "stop": 322798, "observations": { "mutation": [ 321745, 322546 ], "stop": [ 321745, 322546 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024114E65", "query_cov": 1.0, "subject_cov": 0.4009584664536741, "identity": 0.976, "score": 478.0, "evalue": 1.62e-164, "gene_start": 1, "gene_end": 251, "reference_start": 229, "reference_end": 479, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQNWKIVDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEFLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKILFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIRDFEKIDNEQFVKIQSSLNRFNLNYKNINFNAKESLWYGLSILDVKLQDLENIKEISSHLINEIQSLINFLDLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYNKTNATQTLNEILLQKEKIQPDIDWILSRYNQNIFNSEIYKNLDFVKSRNNIFKRIFSKRYRAIKKN" }, "paralog": false, "identity": 0.9778761061946902, "score": 869.0, "evalue": 3.15e-321, "description": "Internal stop codon at 321745, 322546. Nonsense mutation around 321745, 322546.", "psc": { "uniref90_id": "UniRef90_UPI0024114E65", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024114E65", "UniRef:UniRef50_UPI0024114E65" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65" ], "product": "DUF4011 domain-containing protein" } }, "id": "LMJBGEANGM_583", "locus": "GCA013368015_00568" }, { "type": "cds", "sequence": "contig_1", "start": 322544, "stop": 322780, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGTTTCAATTGAAAGTGAAAATGGATTGCAAAATAAAAAAATAATTATTCAAAGCAATGACTTTGTTGTAAATAATTCATTGATAAAGAAATTAAATGATGATTTTGGACTTGATATTAAATTTGAAGCATTTGATAATGTTGAAGGTTCTCTATATGATAGATATTCACAATACATTGAGTTTATAAATCAAAAAATAAATTCCTTCAATGATTCTATAGAACAAAATTGA", "aa": "MPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNVEGSLYDRYSQYIEFINQKINSFNDSIEQN", "aa_hexdigest": "4c295d571bb14942f4d20369c9dace55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9052.916999999994, "isoelectric_point": 4.324731254577638 }, "id": "LMJBGEANGM_584", "locus": "GCA013368015_00569" }, { "type": "cds", "sequence": "contig_1", "start": 322823, "stop": 323182, "strand": "-", "frame": 2, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4", "UniRef:UniRef90_UPI00280BA0F4" ], "nt": "ATGGAAAATAAAGAATTAATTACAAAAACTTTGGCATATGAGTCTTTAAAGAATAAGCTATTAGACACAACATTAAGAAATATATTGATAAATTATAAAAAAAATAAATCATCAACTATAACAATTGAAAAGGAAGACATAAAATCAATTTTAGATAAAATTGAAAATAGAAGTGAATTTAAATTTATTGGTTCTCAAGATTCTGATGATGACATTGAAGTTGAAAGCAATGAATTTTTGTCCTCTGTTTCTCAAAAAGAATTATTAGCAATATTGTCGTTATTGCAAAGAAAGCAAAAGACTTTATTAGAAGAACAAGGAATTAATATTCTATATTTGGCTTTTGGCGGGCTTCAATGA", "aa": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSVSQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLQ", "aa_hexdigest": "bb8d673ee3579c73dc3c951cd7cd6f7f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 322520, "stop": 323182, "observations": { "mutation": [ 322546, 322825 ], "stop": [ 322546, 322825 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280BA0F4", "query_cov": 1.0, "subject_cov": 0.4473684210526316, "identity": 0.983, "score": 211.0, "evalue": 6.74e-67, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLSFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQDWKIIDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSE" }, "paralog": false, "identity": 0.9681818181818181, "score": 409.0, "evalue": 2.54e-147, "description": "Internal stop codon at 322546, 322825. Nonsense mutation around 322546, 322825.", "psc": { "uniref90_id": "UniRef90_UPI00280BA0F4", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280BA0F4", "UniRef:UniRef50_UPI00280BA0F4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4" ], "product": "DUF4011 domain-containing protein" } }, "id": "LMJBGEANGM_585", "locus": "GCA013368015_00570" }, { "type": "cds", "sequence": "contig_1", "start": 323423, "stop": 324154, "strand": "-", "frame": 2, "gene": "tpiA", "product": "Triosephosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "KEGG:K01803", "RefSeq:WP_036432897.1", "SO:0001217", "UniParc:UPI0005138FF4", "UniRef:UniRef100_A0A454C9S4", "UniRef:UniRef50_D1J827", "UniRef:UniRef90_D1J827" ], "nt": "ATGAAATATTTAATTGGTAATTTAAAAATGAATCTATCTTATTTAGAAAGTCAGAATTATTTAAATGAACTACAATCATTAAATAAAATTAAGAATTTTAAAAATGTAATAGTTGGAGCGGCTTTGTCATTCGATGCAATGTCTCTGTCTTTACAATATAATAAAAGAAATTTCTTGTTTGGAGTTCAAAATATTTTTTATAAAGAAAAAGGTGCATACACTGGGGAAGTTTCATTTAGATCAGCAGTTGAATTGAACATTGACTTTGTTTTAATAGGACATAGTGAACGTAGAAAATTATTTAATGAAACTGATCAATTGATTAATTTAAAAATTAAACAAATTGAAAAAACTAGCATTATTCCTATTTTGTGTATAGGGGAAGATCAAGAAAATTGTAGCGATAAAGTCTTTGAGGATATTATAAAAAACCAAATATTAAAGGCTTTAGAAGATGTTGATGTTTATAATCGTTTAATTATTTCATACGAACCTACTTATTGCATTGGAAATGGAAAAATTCCTCAAAATAACCACATCGAAAAAGCAATATCTGTAATTAAAAAAATTACTAATAATCAAGTTCCGGTGTTGTATGGTGGTTCAGTTTCACAAAAAAATATTGAAAAACTGCTTGAAATTAAGGATTTGGATGGTTTTTTAGTTGGCGGAGCCGCATTAGATGCTCAAGGTTTTTTAGAAATGGGTTCAATAATAGAAAAAAGCAACTAG", "aa": "MKYLIGNLKMNLSYLESQNYLNELQSLNKIKNFKNVIVGAALSFDAMSLSLQYNKRNFLFGVQNIFYKEKGAYTGEVSFRSAVELNIDFVLIGHSERRKLFNETDQLINLKIKQIEKTSIIPILCIGEDQENCSDKVFEDIIKNQILKALEDVDVYNRLIISYEPTYCIGNGKIPQNNHIEKAISVIKKITNNQVPVLYGGSVSQKNIEKLLEIKDLDGFLVGGAALDAQGFLEMGSIIEKSN", "aa_hexdigest": "f072e141c61a95602ea3d9cf508ba5ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0005138FF4", "ncbi_nrp_id": "WP_036432897.1", "uniref100_id": "UniRef100_A0A454C9S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005138FF4", "RefSeq:WP_036432897.1", "UniRef:UniRef100_A0A454C9S4" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9S4", "uniref90_id": "UniRef90_D1J827" }, "psc": { "uniref90_id": "UniRef90_D1J827", "gene": "TpiA", "product": "Triosephosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_D1J827", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803" }, "pscc": { "uniref50_id": "UniRef50_D1J827", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J827" ], "product": "Triosephosphate isomerase" }, "genes": [ "tpiA" ], "id": "LMJBGEANGM_586", "locus": "GCA013368015_00571" }, { "type": "cds", "sequence": "contig_1", "start": 324172, "stop": 324390, "strand": "-", "frame": 3, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828", "UniRef:UniRef90_D1J828" ], "nt": "ATGCGTCGCGCAAAAGACAAATTTTATTCAGGAATGACAATTCATCAAAAGGATATTATTATTGCTAAAGAACGCCAAGAATTTGAACGTGAAAGACTCAAAGCTGAAAAGCAAAGAAACTATCAATCAATTAATAATTTATCATTTCTTTTATTAATTTTATATGGATTAATTATATTAATTATATTGATTCCATTTTTAGCATTAAAAATAGTTTGA", "aa": "MRRAKDKFYSGMTIHQKDIIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALKIV", "aa_hexdigest": "01c0fe7e494d664a30d2ab3cc3d159aa", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 324172, "stop": 324690, "observations": { "mutation": [ 324435, 324630, 324642 ], "stop": [ 324435, 324630, 324642 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J828", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 0.986, "score": 124.0, "evalue": 1.69e-34, "gene_start": 1, "gene_end": 72, "reference_start": 102, "reference_end": 173, "reference_sequence": "MTLKQKNFRNQKKSISYWKNAWNKATISYFFVSLVIYIALIFIVRYSKKSIDGQYVHSWQNSLTVSMIFAITINFIIVVYRKGMGKWIVNPIANLIRNRIIMRRAKDKFYSGMTIHQKDIIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALRIVW" }, "paralog": false, "identity": 0.9709302325581395, "score": 319.0, "evalue": 4.65e-114, "description": "Internal stop codon at 324435, 324630, 324642. Nonsense mutation around 324435, 324630, 324642.", "psc": { "uniref90_id": "UniRef90_D1J828", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J828", "UniRef:UniRef50_D1J828" ] }, "pscc": { "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828" ], "product": "DUF3899 domain-containing protein" } }, "id": "LMJBGEANGM_587", "locus": "GCA013368015_00572" }, { "type": "cds", "sequence": "contig_1", "start": 324433, "stop": 324591, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTTATATTGCATTAATTTTTATAGTTAGATATTCAAAAAAATCTGTTGATGGTCAATATGTTCATTCTTGGCAAAATTCATTGACTGTATCAATGATTTTTGCAATTACTATAAATTTTATAATTGTTGTATATAGAAAAGGAATGGGAAAATGA", "aa": "MIYIALIFIVRYSKKSVDGQYVHSWQNSLTVSMIFAITINFIIVVYRKGMGK", "aa_hexdigest": "fe5ef27d68fb9bed388bcaf54785a2dc", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6044.201899999998, "isoelectric_point": 9.9959680557251 }, "id": "LMJBGEANGM_588", "locus": "GCA013368015_00573" }, { "type": "cds", "sequence": "contig_1", "start": 324812, "stop": 325015, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTGATGCAGAAATTAATTCAAATAAAATATTTCAAAAACCAGATGGCCAAGATTTTATTGAAAAATTTATAAACCCATATATTAATAAATTAGTTAATAACGAAAAAGACAGTTCATATACAAATTTCGAACAAGTTGAGCTTGATAAACAAAAATTTAACGAAATTTATCACAAAAAAATAAATTCACTAATCAAATAA", "aa": "MTDAEINSNKIFQKPDGQDFIEKFINPYINKLVNNEKDSSYTNFEQVELDKQKFNEIYHKKINSLIK", "aa_hexdigest": "a897ad6916a1d7de222f4e0c452be238", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7998.895599999998, "isoelectric_point": 5.727744865417481 }, "id": "LMJBGEANGM_589", "locus": "GCA013368015_00574" }, { "type": "cds", "sequence": "contig_1", "start": 325031, "stop": 325915, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2", "UniRef:UniRef90_A0A454C9N2" ], "nt": "ATGAGAAAAAAATTACTTTATATATTGCCGTTATCTTCAACTATTTTAATAACCCCATTAGTTGCAGCTTCTTGTTTTAAAAAACCTGAGCAACCTAAACAAAATATAGAAACCACACAAACTAAAGATGAAAATGAACAAAAATCTGAAGAATTATTAAAAAAATTTAATGATGCAAAAACTGATCTCTTTAGTTATTTAAAAGATAACTTTTTGGTAGAAGGAGCTGAACAATTTAACACCTTAAAAGAAGTTCAGGATGCCAAGAAATTATTGGAATCTTTTAATAAAGAATCCCCTGAATCATCAATAGAAAGTTTAAACGATAAAATACAAAAAATAAACGTTGCTAAACAAAAAATACTTAAAATTCAACCACTTTTAAAAGACTTAACAAAAATTGATAATTTAGTAAACGAATTAAATAATCTTGAAAATAAAATTAAATTAGATGCAGACAATTCTAAAATAGATAAATTAATAAGAAACGAATTAAAATCAATTGCTGATAATTTATCTTTAACTTTAAAGGCAATTAATCTAGAAGCATTATTGAACAATATTAATAGCAAAGATATTGCTAGTTATTTAAATAAGTTGAACGAGATTAAGCAAATAACCTCGCTTAGAATTCAAAAAACTAAATTTATTAATTTAGCATTGCAACAAAAATTCCAAGACCAAGTAATTGAAAATATTATCGATAAAATTGCAAAAAACAATTTAAAAGAAAAATATGTACCATTAATAAATCAATATCAATATTTTAAGGGACAAATTATTGAAAAAATTAACAAATGTCATGCAAATGATAATTTAATAGAATCTTTAAATGAACAAATTAATTCTATAAGTCAAAAGTTAGCATTAAACGAGTCTATTTGA", "aa": "MRKKLLYILPLSSTILITPLVAASCFKKPEQPKQNIETTQTKDENEQKSEELLKKFNDAKTDLFSYLKDNFLVEGAEQFNTLKEVQDAKKLLESFNKESPESSIESLNDKIQKINVAKQKILKIQPLLKDLTKIDNLVNELNNLENKIKLDADNSKIDKLIRNELKSIADNLSLTLKAINLEALLNNINSKDIASYLNKLNEIKQITSLRIQKTKFINLALQQKFQDQVIENIIDKIAKNNLKEKYVPLINQYQYFKGQIIEKINKCHANDNLIESLNEQINSISQKLALNESI", "aa_hexdigest": "04145d126a921f52ec2b24703abbed7e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A454C9N2", "query_cov": 1.0, "subject_cov": 0.8010899182561307, "identity": 0.935, "score": 443.0, "evalue": 7.19e-154, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9N2" }, "pscc": { "uniref50_id": "UniRef50_A0A454C9N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2" ], "product": "Lipoprotein" }, "genes": [], "id": "LMJBGEANGM_590", "locus": "GCA013368015_00575" }, { "type": "cds", "sequence": "contig_1", "start": 326129, "stop": 326794, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175936719.1", "SO:0001217", "UniParc:UPI00158ECB30", "UniRef:UniRef100_UPI00158ECB30", "UniRef:UniRef50_D1J831", "UniRef:UniRef90_A0A6A8Q243" ], "nt": "ATGAGAAATAAAATTCGCTTATTAATACTAAGTGCCGTTGCAACTTCAGTTGTTGCAATGCCATTAGTTGCAGCAGGATGCAAAACAACAAATGATAGTCAAAATTCTACAACCACAGGTGAAGATTCGACTGGACAATCTCAAGATTCTACAACCACAGGTGAAGATTCGACTGGACAATCTCAATCTGGCGACATTATAGATCCTAATAAGAGAGCAAAAGGTCAATTAGGTCAAAGCCAATTGTCATATATTAGAGACCAATTTGGTTTCACTCTAACTAATGAAGGTAAAGAAATATTAAATGACCCTGCACGTGGAACAAAAGAATTACAAAGAATTTTAGAAGGAATAATCAATAAGTATAAATCTATTGATGAACAAATTGAAAACAGCATAAAAAATAAACAAACTAGTGGAATAAATTCGCAAGTTTATAAAGCATATAAAAATGTAATGAATGATGCAGATTTTGTAAAATATTTTAAGGTTACATTGCCTAAGATACTTACTCCGTTTGGCCACCCCATTTCAATACAATTAAAATCAAAGGGAAGATTAAGAGATTTGCCAGTAATTTCTTATAGTGTAATATGCCCTGATAAATCAAATAATGGAATTTTTAGTAGTGATGGTGATAGCGAAGTACCACTTGAAATTGATTAA", "aa": "MRNKIRLLILSAVATSVVAMPLVAAGCKTTNDSQNSTTTGEDSTGQSQDSTTTGEDSTGQSQSGDIIDPNKRAKGQLGQSQLSYIRDQFGFTLTNEGKEILNDPARGTKELQRILEGIINKYKSIDEQIENSIKNKQTSGINSQVYKAYKNVMNDADFVKYFKVTLPKILTPFGHPISIQLKSKGRLRDLPVISYSVICPDKSNNGIFSSDGDSEVPLEID", "aa_hexdigest": "d55b8bfbddba45ee664b1491590c4c0a", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00158ECB30", "ncbi_nrp_id": "WP_175936719.1", "uniref100_id": "UniRef100_UPI00158ECB30", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158ECB30", "RefSeq:WP_175936719.1", "UniRef:UniRef100_UPI00158ECB30" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158ECB30", "uniref90_id": "UniRef90_A0A6A8Q243" }, "psc": { "uniref90_id": "UniRef90_A0A6A8Q243", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J831" }, "pscc": { "uniref50_id": "UniRef50_D1J831", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J831" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_591", "locus": "GCA013368015_00576" }, { "type": "cds", "sequence": "contig_1", "start": 326796, "stop": 327344, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175936720.1", "SO:0001217", "UniParc:UPI00158F5397", "UniRef:UniRef100_UPI00158F5397", "UniRef:UniRef50_D1J832", "UniRef:UniRef90_D1J832" ], "nt": "ATGAATAAAAAGATTACACTATTTTGTTTACCAATAGCTTTAATAATTCCAACAATTGCAGCAGTTTCATGCCAAAAACCCTCTAATTTAAAAGCCAAAAATGATTTATTAAAAACCAAGATTATTGAAGGAATTAATTGCCCATGTCATACTAAATATAATGTTTTATCTAATAATAATCAAAACATGTCTAGTACTATGCTTGCAGCAATTTATGATAGTTTTAATTTTGAATCCAAAATACCGCTTAATCAAGAGAATAAGAAATTTTTATTCAAAATCATTGATAAACTCAATGATAAATACAAAAAAGATGATCAAAAGTCAAACATATTCAGTGATGAGTTATTTTTAAAATATTTTAAAATTTCATTGCCTGATTCGAAATACTTATTAAATCATACATTAAAATTAGAACTTGGATATGGTGATACAAATTTAAAATCAGATACTATATTTATAAAATATAGTATAATATGTAAATTAAGAAGTAACCAATTTTTACAAGTTATTGAAGATGAACGATATGTTTATATTCAGGGGGAATAA", "aa": "MNKKITLFCLPIALIIPTIAAVSCQKPSNLKAKNDLLKTKIIEGINCPCHTKYNVLSNNNQNMSSTMLAAIYDSFNFESKIPLNQENKKFLFKIIDKLNDKYKKDDQKSNIFSDELFLKYFKISLPDSKYLLNHTLKLELGYGDTNLKSDTIFIKYSIICKLRSNQFLQVIEDERYVYIQGE", "aa_hexdigest": "e85e8058d2b3aa35fa226784d05d477b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158F5397", "ncbi_nrp_id": "WP_175936720.1", "uniref100_id": "UniRef100_UPI00158F5397", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158F5397", "RefSeq:WP_175936720.1", "UniRef:UniRef100_UPI00158F5397" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158F5397", "uniref90_id": "UniRef90_D1J832" }, "psc": { "uniref90_id": "UniRef90_D1J832", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J832" }, "pscc": { "uniref50_id": "UniRef50_D1J832", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J832" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_592", "locus": "GCA013368015_00577" }, { "type": "cds", "sequence": "contig_1", "start": 327344, "stop": 327847, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_036438522.1", "SO:0001217", "UniParc:UPI000512E14A", "UniRef:UniRef100_A0A454C9K7", "UniRef:UniRef50_D1J833", "UniRef:UniRef90_D1J833" ], "nt": "ATGAAAAAAATAATATTATCTATAGGGGCAGTTCTAGTTTTAGCTTCGCCAATAATTTCAGCATCTTGTGTTTCAACAGATGAAAATACAGCAAAAGCAAATGAAATTTACACTAATAAAAGTGGAATTTTTAATTCTTCTCAATTAGAAGCAATTAAAAATGATTTTGTTTTCGAATTAACTGAACAGAGCAAAATGCTAAAAAATAATTATGGCAATAAGGCGTTGGCAGATGAGCTAAAAAAGATTTGTAAAGATTATGAATTGCAAATTAATCTTAGCCCTTCTGAAAATAATAAATTAGCAGGTTTGAGGTTAATTAATAATGCAAACTTTTTAAAATTGTTTAAAGTGGTTAAGCCGAATTTAGGTGTAAATCACCAATTGATAATTAATTTTAAAATTGATAATAATAACAATATTTCATTAATCTATGACATATATTGCAAAGATGTAAAAACATATGATGCCAAAGATCAAGAAATAAAATTAGATTTAGAATAA", "aa": "MKKIILSIGAVLVLASPIISASCVSTDENTAKANEIYTNKSGIFNSSQLEAIKNDFVFELTEQSKMLKNNYGNKALADELKKICKDYELQINLSPSENNKLAGLRLINNANFLKLFKVVKPNLGVNHQLIINFKIDNNNNISLIYDIYCKDVKTYDAKDQEIKLDLE", "aa_hexdigest": "892330127ef8a105eaa97d96e0769221", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512E14A", "ncbi_nrp_id": "WP_036438522.1", "uniref100_id": "UniRef100_A0A454C9K7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512E14A", "RefSeq:WP_036438522.1", "UniRef:UniRef100_A0A454C9K7" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9K7", "uniref90_id": "UniRef90_D1J833" }, "psc": { "uniref90_id": "UniRef90_D1J833", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J833" }, "pscc": { "uniref50_id": "UniRef50_D1J833", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J833" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_593", "locus": "GCA013368015_00578" }, { "type": "cds", "sequence": "contig_1", "start": 327857, "stop": 328705, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834", "UniRef:UniRef90_D1J834" ], "nt": "ATGGTAAACATTTTTAAGCCATTGATTCCAAAAAGTGTTCATGAAAATAGTCTTGACTTTATTGACAATAGTTATTATCTTGCAAACAAAAAAATATTAAACGATCTTCAATCAAGCAATCTTGATTACAAATTAAAATCAGCTTATTTTCAAGAAATATCTAAACAACTAAATGATATAATAAAAAAGTTTTATGAACAATATCCAGATGCTGCTGATATTGAATTTGATATTCCACTAAATTTTTTGAATTTGCAATTTAATAACCAAAGGCAAAATGTTTTAGAACATATCAAAACAATATTTAAAACAGTAAATAAACATTTAAAGCCAAATTTTGTAGTTATTGATGATTTAGCAAAATATGACTTGTATTTTAATCAAGATGCTTCAATATATTGCAATAATAGCTTTGAACTTTTAAAATCAAATACTAAAGAATTTATAGCAACAGCCATTGCTAAAAACTATAAATTGTTTTTAATCTTTAATAAAATTTCTAGCTTAACTAAAAGCAATAGTTTTATTCTAGAAAATACCAAAAAAATATTAAATGAATTAAATAAAACAAAACTAATTACAAATAATGAAATTTTTAACAAAAATTTAGAAGATTACAACAAAAATTTCTTTATAATAAAATCAATTATATTAAAATACTTAGAAAATATCTCTTACCAAGACCAATTAAATATAATTAATGATATTAACAGTTTACTTTCTTATACCTTTTCATTTGAAAATATCATTAAAACTGTTGGTTTTTCAAAAGTAATATACCAAAAATTTATTAATAAACCAATTAGTTATGACGGGTTGTCTTACTTGCAAGATATTGTAATAGAATAG", "aa": "MVNIFKPLIPKSVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQYPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKTVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFFIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE", "aa_hexdigest": "6e55abb1c3647464d812ab841414e008", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 327857, "stop": 329005, "observations": { "mutation": [ 328783, 328972 ], "stop": [ 328783, 328972 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J834", "query_cov": 1.0, "subject_cov": 0.6482758620689655, "identity": 0.9890000000000001, "score": 541.0, "evalue": 1.13e-191, "gene_start": 1, "gene_end": 282, "reference_start": 154, "reference_end": 435, "reference_sequence": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITINWMPTANKKFFNDSFSMLAYGLKANQLNENTNINKILKSKNIAFLLLIHQLINQYGMLANQGDIWTSQAPSDLDISSFDSGINYHVLKDSMVNIFKPLIPKNVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFFIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE" }, "paralog": false, "identity": 0.9842931937172775, "score": 740.0, "evalue": 1.24e-272, "description": "Internal stop codon at 328783, 328972. Nonsense mutation around 328783, 328972.", "psc": { "uniref90_id": "UniRef90_D1J834", "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J834", "UniRef:UniRef50_D1J834" ] }, "pscc": { "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33192.69550000005, "isoelectric_point": 8.276725196838385 }, "id": "LMJBGEANGM_594", "locus": "GCA013368015_00579" }, { "type": "cds", "sequence": "contig_1", "start": 328781, "stop": 328969, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGACTGCAAATAAAAAATTTTTTAATGATAGTTTTTCAATGCTAGCATATGGTTTAAAAGCAAACCAATTAAATAAAAATACGAATATAAATAAAATATTAAAATCCAAAAATATTGCCTTTTTATTATTGATTCACCAATTGATTAATCAATATGGGATGTTGGCTAACCAAGGTGATATTTGA", "aa": "MPTANKKFFNDSFSMLAYGLKANQLNKNTNINKILKSKNIAFLLLIHQLINQYGMLANQGDI", "aa_hexdigest": "874cce999f854d5d9ded8bcbe3ad6090", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7029.1702, "isoelectric_point": 9.903326988220215 }, "id": "LMJBGEANGM_595", "locus": "GCA013368015_00580" }, { "type": "cds", "sequence": "contig_1", "start": 328970, "stop": 329164, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGATATCAAAAAATACTTTTGGTATGCAAATGTATCATGCATTCTTGCAAAATATTGTAACTACATTGAATTTTGACGATCTTGACATAAATCAACAACAAGAAATTTTGCATAAATATAATGATTTTAATATAAGCTATACTCGTAATGTTTCTAAAAATAAGTTGAGTAATAATATTACCATTAATTGA", "aa": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITIN", "aa_hexdigest": "3364ab9bd125409e701de2e7bc69a378", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7553.4361, "isoelectric_point": 6.698270606994629 }, "id": "LMJBGEANGM_596", "locus": "GCA013368015_00581" }, { "type": "cds", "sequence": "contig_1", "start": 329723, "stop": 330286, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAACATTCTTATTTTAAGTTCAATGTTAGCATTATTTACAATGACATCTGCGATATCAATATCTTGTAAATTTAATAATTTTAATGAAGATAAGAAAAAATATATTCAAAAGCTTAATATTGCAAATCCTTATTTAGAATATGGCAGTTCAATACCAAATTTCAAAAACCAAAATTTAAAAGAAATAAATTTAATGACAGGAGCCAAAGTTCTTAGAATTTATTCAAAAAATCAACCTAAAATAGACTTTAGGGATAATATAGTTTTGCGCCCGACTGAATTAAGTTATAAATTTGAATATGGTAATAGTATTCAAATAAATGATAAACTTTTCAATAGCGATGATAAACTAAATGTTGACTATCAAAACACTGAATTGAATAAGCAAGATCCCAACAAATATTACCCCAGCAAAGACAAGGGCAATGGTTTTAATAATCCATATTTGTTCGTTCCTTCAAAAAATTCAATGTCTATAAATAGCAAACAATTCATTGAATCTCTAAGAAAAGCAAAAAATATTTCAATCAGTATAAATCAAAGAAAAAATTATTGA", "aa": "MKKNILILSSMLALFTMTSAISISCKFNNFNEDKKKYIQKLNIANPYLEYGSSIPNFKNQNLKEINLMTGAKVLRIYSKNQPKIDFRDNIVLRPTELSYKFEYGNSIQINDKLFNSDDKLNVDYQNTELNKQDPNKYYPSKDKGNGFNNPYLFVPSKNSMSINSKQFIESLRKAKNISISINQRKNY", "aa_hexdigest": "087f6aa4bd71659b426c1c56cf903b00", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21767.671700000003, "isoelectric_point": 9.670080375671386 }, "id": "LMJBGEANGM_597", "locus": "GCA013368015_00582" }, { "type": "cds", "sequence": "contig_1", "start": 330492, "stop": 330773, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATGAGTCATAGAATTATTGATGGACTATTTTTGGGATATAATGTTTATACATTAACATCAATTGGTGGAACATATAAAGCAAATATTCCTTTAATAATAACATTTAATATTCATATATTACTTGAAATTTTAGTTGTTTATTATCGTCAAATTCAATATGGAGAAAAGAAATCAAAAGCCATACTATACAACTTCTTAACATTTTTATTAATTATCCCATTTATGTTAATTGGCGGATTGCTTGGTCAATATATTGAACATAGTAAAGTGTTTTGA", "aa": "MLMSHRIIDGLFLGYNVYTLTSIGGTYKANIPLIITFNIHILLEILVVYYRQIQYGEKKSKAILYNFLTFLLIIPFMLIGGLLGQYIEHSKVF", "aa_hexdigest": "6c261c62e31d345c939dfab4a8d16dc6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10745.78719999999, "isoelectric_point": 9.260512733459471 }, "id": "LMJBGEANGM_598", "locus": "GCA013368015_00583" }, { "type": "cds", "sequence": "contig_1", "start": 331014, "stop": 331241, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATAGGAACAAGTGCATTTAATGGAAATATAACTTTAGCAACATTTGTAAATCTAATAATCTATATTTTAATATTATTGGGCGTACCTTCATTAGTTTTGGGCGTAATTTCTATATTTAAATTAAAAATTACTGATAAGAAAAATATTTATATTTATGCATTTGCTACGGGAATGTTTCTAATGATTGGCACTGTAGGTTTCTTTAAAGAAAGTTTTGATTGA", "aa": "MFIGTSAFNGNITLATFVNLIIYILILLGVPSLVLGVISIFKLKITDKKNIYIYAFATGMFLMIGTVGFFKESFD", "aa_hexdigest": "2dc2ffb6a1c8af9c24258720723a8558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8289.930899999998, "isoelectric_point": 9.158330345153807 }, "id": "LMJBGEANGM_599", "locus": "GCA013368015_00584" }, { "type": "cds", "sequence": "contig_1", "start": 331322, "stop": 331789, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCTCACTTAGCTTTATGCCTTTAATTATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAGTTTTTTTGCATAGGCTAAGCATAGGTCAGATTGCTGCATTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGCGATATTAATAATTTTTTAAACCAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGA", "aa": "MKKKFYFNSILIASSLSFMPLIICSCQTKKDNYIIEGSNAYIDISKISRVFLHRLSIGQIAALHNMYKIFYYYDNSNKKQYYDSAYVNGQKLYLRNANKNVEYKLNFPIKPSWKQEISKFDNINIVKSNKPSDINNFLNQYSFDEVDSAGDVNDE", "aa_hexdigest": "8b6837c93bd8170c500ec0e8e42a4ca7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18136.416099999995, "isoelectric_point": 9.039644050598145 }, "id": "LMJBGEANGM_600", "locus": "GCA013368015_00585" }, { "type": "cds", "sequence": "contig_1", "start": 331856, "stop": 331999, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTGTTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGA", "aa": "MQTVIFRLIQDTELNYSIMKSKYLINSKRENVITQNLFKNKYIQASS", "aa_hexdigest": "4154c14e804a354ed385c6b44658f08f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5613.466700000002, "isoelectric_point": 9.775808525085449 }, "id": "LMJBGEANGM_601", "locus": "GCA013368015_00586" }, { "type": "cds", "sequence": "contig_1", "start": 332435, "stop": 332857, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTTTTCAAAGCTTCAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCTGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACTCCATATTTTAAAACAAATCAAATTTTTAAAGCAATAGTAAGAGTATTTAAACTAAATGGTACATATAAAGATTATGTTTTAATATCTTCCAATTTTGATGATCACGGTCACAGATTAAAAGGTTTAATTACTAAAAATGCTTTGCCAAATGAACTTAAAGCTTCTGATATATATTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTAGATGATTTCATTGATAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGA", "aa": "MYLFQSFKNEPGFFKLAVPEYKKNEDKEYKIIDFEYTPYFKTNQIFKAIVRVFKLNGTYKDYVLISSNFDDHGHRLKGLITKNALPNELKASDIYSIRADSEPIKQGIKLDDFIDKNNKNSVFRYMLNESAKKLENIFEY", "aa_hexdigest": "334cd2664ba84e5911bb83b26f45ccc0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16604.766499999987, "isoelectric_point": 8.872735404968264 }, "id": "LMJBGEANGM_602", "locus": "GCA013368015_00587" }, { "type": "cds", "sequence": "contig_1", "start": 332894, "stop": 333154, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGCTTGCCAAAGATCCATTCCAATTAAAATTGTTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCACTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTACCAAATCAACCAATTGGAAGATTATATTTAAAGATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGA", "aa": "MLLAKDPFQLKLLASYLNNYLLAYALENKAGEVFSGIKRIDLNIVKLPNQPIGRLYLKMDFVSYANNQDFKFKSKNEKIVKSVYLY", "aa_hexdigest": "3ebe5440d794178d82403b1ba6fa47e8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9995.658199999994, "isoelectric_point": 9.58440189361572 }, "id": "LMJBGEANGM_603", "locus": "GCA013368015_00588" }, { "type": "cds", "sequence": "contig_1", "start": 333238, "stop": 333615, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCAATAAAATTGCTATCCTTGCTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCACGATAACGAAGACAAGGAAAATTTAGATTTTTCAAGTGATTTTAGTGGCAAAAAGCAAAAAAATGAATTCAATTTCAAGAATAAGATTTCCCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAACACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAATTGA", "aa": "MKKSIKLLSLLCTSTIISLPAISCTIKVNHDNEDKENLDFSSDFSGKKQKNEFNFKNKISQNIYDLYQNKIAILFNDVKTNYRGYKKIFGSLTRNLNTLRAKINSLRYEQSLKENKKILNDFYNN", "aa_hexdigest": "44f8f9321ebd60c40bb3a5603820618c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14610.592599999982, "isoelectric_point": 9.645195579528806 }, "id": "LMJBGEANGM_604", "locus": "GCA013368015_00589" }, { "type": "cds", "sequence": "contig_1", "start": 333634, "stop": 333831, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCTGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAAACAATTGATGACAGACTTGAAGGTAAAGACATTGATTTAGGTCAAACTCAACATGCATTACAAGCGGTTTGA", "aa": "MLKNNPFALYLFKYSLIFSDVDAVLADTNLVFETSEFLKYLKTIDDRLEGKDIDLGQTQHALQAV", "aa_hexdigest": "53ee0f7517b2a8fe14d8b8609555d331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7441.420799999998, "isoelectric_point": 4.562545585632326 }, "id": "LMJBGEANGM_605", "locus": "GCA013368015_00590" }, { "type": "cds", "sequence": "contig_1", "start": 333883, "stop": 334122, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGATAAAATTAACATTGAAGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTAGCAAAAAATAAACTTCAAAATTACGAAAAATTTCAAGAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAAATTGA", "aa": "MLDKINIEDDKNSHNHSHAIINLTYEMGLWHEMLAKNKLQNYEKFQEEYKIAKKHIIDNINNIDVNNNYLALKKVLDKN", "aa_hexdigest": "3a8cea4079f01616978c757840637aba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9436.63479999999, "isoelectric_point": 6.496264839172363 }, "id": "LMJBGEANGM_606", "locus": "GCA013368015_00591" }, { "type": "cds", "sequence": "contig_1", "start": 334572, "stop": 334943, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCAATTGATAAAAATATTGACGAAAACGTGGGAATTTTTAGAAAATCTTCATTTAATTCACTTTATCATAAAATTTTAACGATCATGTCTAATACACATTGTTCATTTGAAGAAAAAATGGAAACAATTAAAAAAGTTTTATTAAGCAAAGGAATTAATCTAGTAACGATGCCTTTTATTGAAAATAGTTTGATAAGGGCTATTGCTTTGAAAAAAGGAGCAAAAAGGTATATTTTCCTTTCTGATATGTTTAATTCTGAAGCATATATATTTTATTCATTGTTACATGAAATAGTTCATTGCTTTTTTTCAAATTATACTGAAAACAAAATTGATAAAGTTGTTATCAAAGAATATTTTGCATGA", "aa": "MLSIDKNIDENVGIFRKSSFNSLYHKILTIMSNTHCSFEEKMETIKKVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFA", "aa_hexdigest": "ba766b8f422c6f6330882b0fbe770af8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.700799999982, "isoelectric_point": 8.832313728332519 }, "id": "LMJBGEANGM_607", "locus": "GCA013368015_00592" }, { "type": "cds", "sequence": "contig_1", "start": 334989, "stop": 335471, "strand": "-", "frame": 1, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844", "UniRef:UniRef90_D1J844" ], "nt": "ATGATTGAAGAGCACATCTGTGATATGAAGCACGTTAAAATTCATGGCAATGATTTTACAGATGAAATAAAATACTATTTAAAAATGTTTTCAATGACTCAAAAAGAATTATCTATAAGACTTGGATTATCAATAAAACATATCAATTCAATTATGAATAATGAAGTTAATAACGTTAGTGCAACTATTATTGAAGCGCTTGAATATGCATTTCACTTAGAAATTGGCACATTAACGGAAGTTTATCATATTTATAATAACTTAAAAACACTAAAGAAAAATAAGAATATTGAAGATGATTTAAAGAAACATGGTATTGATTTTTTAATAAATCATCCAGAACTTGCATTGGCAGCTAACATTAGCATTCATGAAAATAGTCCACTACATGTTAAATTAATAATGCTAAAAAGATTTTTTGGTGTTGCTGACTTGGCTAACTATGATAAATATTTAAAAGATAATGTTTCAGCAGAAGAATGA", "aa": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEE", "aa_hexdigest": "e07654f3e13b12c233b1754b5b25cdd6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 334686, "stop": 335471, "observations": { "mutation": [ 334961, 334991 ], "stop": [ 334961, 334991 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J844", "query_cov": 1.0, "subject_cov": 0.4456824512534819, "identity": 1.0, "score": 322.0, "evalue": 1.35e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEEWTYSNPNTKVWIRFCELLSIDKNIDENVGIFRKSSFNSLYHKILTIMSNTHCSFEEKMETIKKVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFAWEKNNPTTYKAIYDAINSYQQCEAIQKANKNIDTSYIWSTLKDKYPYVAFDPLSDQKSKN" }, "paralog": false, "identity": 0.9923371647509579, "score": 512.0, "evalue": 5.85e-186, "description": "Internal stop codon at 334961, 334991. Nonsense mutation around 334961, 334991.", "psc": { "uniref90_id": "UniRef90_D1J844", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J844", "UniRef:UniRef50_D1J844" ] }, "pscc": { "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844" ], "product": "HTH cro/C1-type domain-containing protein" } }, "id": "LMJBGEANGM_608", "locus": "GCA013368015_00593" }, { "type": "cds", "sequence": "contig_1", "start": 335646, "stop": 336041, "strand": "-", "frame": 1, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0008270", "GO:0009972", "KEGG:K01489", "RefSeq:WP_036438513.1", "SO:0001217", "UniParc:UPI0005144531", "UniRef:UniRef100_A0A454C9K2", "UniRef:UniRef50_D5E4U5", "UniRef:UniRef90_A0A454C9K2" ], "nt": "ATGACAATAGAAGAATTAAAAAATAAATTAAAATATTCATTTAGTCCTTATTCACATGTTCAAGTTGCATGTTGTGCAATTGAAGAAAATGGTAAAGAATATTGGGGAGTCAATACTGAAAACGCTGCATTTCCATCTGGATTATGTGCTGAAAGAAGTGCATTATTTGGTTCTGTAGCATATGGCGCTAGAGTTGGTAATTTTAAAGAAATACATATAATTAGCAATTTATCAAAATTGCTATATCCTTGCGGTGCTTGTGCTCAAGTTATTAGTCAATTTTTAAAACCAGATGGCGATGTATATTTATACAGTTTTGATTTAAAGCAACAAAAGCATTATAAAATGAAAGATATTCTACCTTTTGCAGTTGCACCAAAAGATATAGTGGATTAG", "aa": "MTIEELKNKLKYSFSPYSHVQVACCAIEENGKEYWGVNTENAAFPSGLCAERSALFGSVAYGARVGNFKEIHIISNLSKLLYPCGACAQVISQFLKPDGDVYLYSFDLKQQKHYKMKDILPFAVAPKDIVD", "aa_hexdigest": "675e8b3d310036b1f9d0a200199bd6cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005144531", "ncbi_nrp_id": "WP_036438513.1", "uniref100_id": "UniRef100_A0A454C9K2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005144531", "RefSeq:WP_036438513.1", "UniRef:UniRef100_A0A454C9K2" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9K2", "uniref90_id": "UniRef90_A0A454C9K2" }, "psc": { "uniref90_id": "UniRef90_A0A454C9K2", "gene": "Cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_D5E4U5", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0008270", "GO:0009972" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4U5" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "LMJBGEANGM_609", "locus": "GCA013368015_00594" }, { "type": "cds", "sequence": "contig_1", "start": 336193, "stop": 336450, "strand": "+", "frame": 1, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_012855534.1", "SO:0001217", "UniParc:UPI0001BC2233", "UniRef:UniRef100_D1J846", "UniRef:UniRef50_B3PMA1", "UniRef:UniRef90_D1J846" ], "nt": "ATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCTGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGGACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAA", "aa": "MVKLRLKRTGKKFYATYKIVAADARSPRDGKFIEELGYYDPNSKKLVLNKELIIKHLDNGAKPSDTVRNLLKQDNFYADYVASKK", "aa_hexdigest": "b48a36ad0c503e23671ac76fd94afdb2", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2233", "ncbi_nrp_id": "WP_012855534.1", "uniref100_id": "UniRef100_D1J846", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2233", "RefSeq:WP_012855534.1", "UniRef:UniRef100_D1J846" ] }, "ips": { "uniref100_id": "UniRef100_D1J846", "uniref90_id": "UniRef90_D1J846" }, "psc": { "uniref90_id": "UniRef90_D1J846", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_B3PMA1", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMA1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [ "rpsP" ], "id": "LMJBGEANGM_610", "locus": "GCA013368015_00595" }, { "type": "cds", "sequence": "contig_1", "start": 336463, "stop": 337065, "strand": "+", "frame": 1, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "COG:COG0336", "COG:J", "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "SO:0001217", "UniRef:UniRef50_A9NHF7", "UniRef:UniRef90_D1J847" ], "nt": "ATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAAATTATTCATTAGATAAACATCATAAAGTTGATGATAGCCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATTGAACCAATAGATTTAGCACTACAACCTTTAAAAAATAGTTACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCAAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTAGATGAAGAAATATCAATTGGAGATTTTATCTTAACAGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATATACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGA", "aa": "MKINILTLFPDYLNAFCNQSMIAKALELGHIEINIINFRNYSLDKHHKVDDSPYGGGAGMLLQIEPIDLALQPLKNSYKILLSPQGKVFSQKKAHELAKLNEITLICGHYEGFDERITKLVDEEISIGDFILTGGEIAAMAISEAVARLIPGVLRKNSYENESFEGNGLLDYPQYTRPAVYKGMKVPKILLSGNHLEIEK", "aa_hexdigest": "6c0f2ee5b99c95e972cd4e039b039915", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J847", "query_cov": 1.0, "subject_cov": 0.9009009009009009, "identity": 0.995, "score": 400.0, "evalue": 1.1e-140, "valid": true, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_A9NHF7", "cog_id": "COG0336", "cog_category": "J", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_A9NHF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NHF7" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "LMJBGEANGM_611", "locus": "GCA013368015_00596" }, { "type": "cds", "sequence": "contig_1", "start": 337133, "stop": 337489, "strand": "+", "frame": 2, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_012855536.1", "SO:0001217", "UniParc:UPI0001BC2235", "UniRef:UniRef100_D1J848", "UniRef:UniRef50_Q044E2", "UniRef:UniRef90_D1J848" ], "nt": "ATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGTACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGGGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGTAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCAAGATTAAAAGAAATTAAAAACTCAAAATAA", "aa": "MRTKLLELVEKDQIREDLPQLREGYNVRVHVRIKEGDKERIQVFEGLIIAEYGTGINKSITVRKESYGVGVERIFKINSPLIAQIEVIRRNKVRRAKLYYMRDLKGKSARLKEIKNSK", "aa_hexdigest": "9c890839eeb918280e6bf122bb6ab676", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2235", "ncbi_nrp_id": "WP_012855536.1", "uniref100_id": "UniRef100_D1J848", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2235", "RefSeq:WP_012855536.1", "UniRef:UniRef100_D1J848" ] }, "ips": { "uniref100_id": "UniRef100_D1J848", "uniref90_id": "UniRef90_D1J848" }, "psc": { "uniref90_id": "UniRef90_D1J848", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q044E2", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q044E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q044E2" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "LMJBGEANGM_612", "locus": "GCA013368015_00597" }, { "type": "cds", "sequence": "contig_1", "start": 337700, "stop": 337810, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATACAAAAATTTTATTAGCATTAGGAAGCATTGCAACTTCAGTTGCCGCAATACCATTAGTTGCTGCAGGTTGCGTAAAAACAAAAAACAGAAGAACCTAA", "aa": "MKKHTKILLALGSIATSVAAIPLVAAGCVKTKNRRT", "aa_hexdigest": "7cded750917886505923bf806cc227ef", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3762.5806000000002, "isoelectric_point": 11.224670982360838 }, "id": "LMJBGEANGM_613", "locus": "GCA013368015_00598" }, { "type": "cds", "sequence": "contig_1", "start": 339123, "stop": 339533, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGACTCAACACAATGAAAATAAAACCAAAAGAATTGCCCAAAAAAAATTAGCTTTTGAATTGGTTAAAGACATTCATGGAGTTGATATAGCAAACAAATGTATTAGAATAAGTGAGATTTTATTCAACAAAAGTAACGAAGAATTAAACATAGAAGATATTTCGATGCTTTTAGGCTATTTACCATGTATTGGAGTTAAAGAAGGCGAAAAATTTATTGAAATTTTAAAAAATAATCAAATTGTTTCTTCGAATAGAGAAGCAAGAGAATTTATTTCAAAAAATACTTTTTTAATTGATAACAATATAATAAACGATGAAAATTATTCAATTACATTTAATAAATTTAATGGTCAATATGCGCTATTAAAAAAAGGTAAAAAAGACTTTTACATACTTAAAAGAATTTAA", "aa": "MTQHNENKTKRIAQKKLAFELVKDIHGVDIANKCIRISEILFNKSNEELNIEDISMLLGYLPCIGVKEGEKFIEILKNNQIVSSNREAREFISKNTFLIDNNIINDENYSITFNKFNGQYALLKKGKKDFYILKRI", "aa_hexdigest": "c4b0e78999713381a24df08755ee940b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 76, "aa_cov": 0.5441176470588235, "hmm_cov": 1.0, "evalue": 9.305040129156278e-14, "score": 46.700836181640625, "start": 59, "stop": 132 } ], "seq_stats": { "molecular_weight": 15842.142099999983, "isoelectric_point": 9.0186918258667 }, "id": "LMJBGEANGM_614", "locus": "GCA013368015_00599" }, { "type": "cds", "sequence": "contig_1", "start": 339570, "stop": 339728, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTCAATTTATTAGTTGATGAAAATGGTAAAAAATTTGGTAAAAGCACTGGCGGAGGTTCGTTATGGCTTGATCCAAATATGACTAGTCCATATTCTATATATCAATTCTTGTTGAATCAAAGTGATGTAAAAATCGAAGAATATTTAAAATGA", "aa": "MTFNLLVDENGKKFGKSTGGGSLWLDPNMTSPYSIYQFLLNQSDVKIEEYLK", "aa_hexdigest": "4f8361fe08f003b759ad8e2e669c4a83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5918.6582, "isoelectric_point": 4.992987251281736 }, "id": "LMJBGEANGM_615", "locus": "GCA013368015_00600" }, { "type": "cds", "sequence": "contig_1", "start": 339993, "stop": 340358, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ], "nt": "ATGCAAAAAAATATATTAGATGATTTGAAATCAAGGGGTGTTTTAAATAATATATCTAGTGAAGAAAAATTTAATAATATATCACTAGGAACTGGTGTTTATACAGGTTTTGATCCAACTGCTAAAAGTTTACACCTTGGAAATTATCTACAAATATTAAGTTTGTTACGTTTTAAAAAAGCAGGATATAAACCAATTGCAATATTGGGTGGAATAACCGGAATGATTGGTGATCCTAGTTTTAGAAATTCTGAAAGAAAATTTCTAGATCAAGAAACATTACTACAAAACAAAAATGCAATTAAAAATCAATTACAAAAATTTGGTTTAGAAGTAATTGATAATTTAGATTTTTATAAGCATTGA", "aa": "MQKNILDDLKSRGVLNNISSEEKFNNISLGTGVYTGFDPTAKSLHLGNYLQILSLLRFKKAGYKPIAILGGITGMIGDPSFRNSERKFLDQETLLQNKNAIKNQLQKFGLEVIDNLDFYKH", "aa_hexdigest": "4fd057b6db23b16aef4ac9231c5c4af3", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FEF080B", "query_cov": 0.9752066115702479, "subject_cov": 0.9752066115702479, "identity": 0.6609999999999999, "score": 157.0, "evalue": 2.11e-47, "valid": false, "product": "tRNA synthetases class I (W and Y)", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_UPI002FEF080B", "kegg_orthology_id": "K01866" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEF080B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13633.498899999982, "isoelectric_point": 9.362501716613771 }, "id": "LMJBGEANGM_616", "locus": "GCA013368015_00601" }, { "type": "cds", "sequence": "contig_1", "start": 340692, "stop": 341363, "strand": "+", "frame": 3, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "COG:COG1307", "COG:I", "SO:0001217", "UniRef:UniRef50_A0A077L663", "UniRef:UniRef90_D1J851" ], "nt": "ATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAATCTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTCGATGAGGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCAGAGTTTAACGATGCCTTACTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAATGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAAACAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCGTTTAAACAAATAACCAATAATTTTCCAAGGATTTATACAATTCAATTGAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCTGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATACTTAAAAAAACAATAA", "aa": "MQVIEKYFNDYDKILIYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ", "aa_hexdigest": "64ba85468a86c9c466bfb6596a553e58", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 340482, "stop": 341363, "observations": { "mutation": [ 340548, 340635 ], "stop": [ 340548, 340635 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J851", "query_cov": 1.0, "subject_cov": 0.7610921501706485, "identity": 0.996, "score": 432.0, "evalue": 5.63e-152, "gene_start": 1, "gene_end": 223, "reference_start": 71, "reference_end": 293, "reference_sequence": "MKIKIITDSSAGMSQEQANSFGWDLIPIQIEVGNDIYKSGIDFDTDKFEELWRNDKKIDAKTFATPPGIAMQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ" }, "paralog": false, "identity": 0.9863481228668942, "score": 567.0, "evalue": 1.45e-207, "description": "Internal stop codon at 340548, 340635. Nonsense mutation around 340548, 340635.", "psc": { "uniref90_id": "UniRef90_D1J851", "gene": "DegV", "product": "DegV family protein", "uniref50_id": "UniRef50_A0A077L663", "cog_id": "COG1307", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J851", "UniRef:UniRef50_A0A077L663", "COG:COG1307", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L663" ], "product": "DegV family protein" } }, "id": "LMJBGEANGM_617", "locus": "GCA013368015_00602" }, { "type": "cds", "sequence": "contig_1", "start": 341404, "stop": 341667, "strand": "-", "frame": 3, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_012855540.1", "SO:0001217", "UniParc:UPI0001BC2239", "UniRef:UniRef100_D1J852", "UniRef:UniRef50_O21275", "UniRef:UniRef90_D1J852" ], "nt": "ATGGCACATACGAAATCAGGTGGAACAACCGCCAATAGCCGTGATTCAGCGGGAAGAAGATTAGGCGTAAAAGCAACCGACGGCCAATTTATTTTAGCAGGAAGCATTATTTATAGACAAAGAGGAACAAAAATTTTCCCAGGCCAAAATGTTGGTAGAGGAAAAGATGACACCTTATTTGCTTTAATCGATGGTATTGTAAAATTCGAAAGCAGAATTAACAGAAAATTTGCATCAGTTTACCCAATTGAAAGCAAGAAATAA", "aa": "MAHTKSGGTTANSRDSAGRRLGVKATDGQFILAGSIIYRQRGTKIFPGQNVGRGKDDTLFALIDGIVKFESRINRKFASVYPIESKK", "aa_hexdigest": "8bf9087f30d2afc010cb018fb10ca22d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2239", "ncbi_nrp_id": "WP_012855540.1", "uniref100_id": "UniRef100_D1J852", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2239", "RefSeq:WP_012855540.1", "UniRef:UniRef100_D1J852" ] }, "ips": { "uniref100_id": "UniRef100_D1J852", "uniref90_id": "UniRef90_D1J852" }, "psc": { "uniref90_id": "UniRef90_D1J852", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_O21275", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmA" ], "id": "LMJBGEANGM_618", "locus": "GCA013368015_00603" }, { "type": "cds", "sequence": "contig_1", "start": 341670, "stop": 341972, "strand": "-", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855541.1", "SO:0001217", "UniParc:UPI0001BC223A", "UniRef:UniRef100_D1J853", "UniRef:UniRef50_Q6F123", "UniRef:UniRef90_D1J853" ], "nt": "ATGTTTGCTATTATTGAAACCGGCGGAAAACAATTACTTGTTAAAAGCGGTGATGTAATTTACATCGAAAAAATTGATGGCAATGAAGGTGACAAAGTTTCATTTGATAAAGTTTTAGTTATTGATGAACAAGTTGGCTCACCTTATCTACCAAAAGCTTCTGTTTTAGGAACCATTGAAAAACAAGGAAAAGCAAAAAAAATAGTTGTTTATAGACATAATGCTAAATCTACTCATAAACGTAAATTAGGACATCGTCAACCATTTACCAGAGTTAAAATTACAGAAATTAAGGAAAAATAG", "aa": "MFAIIETGGKQLLVKSGDVIYIEKIDGNEGDKVSFDKVLVIDEQVGSPYLPKASVLGTIEKQGKAKKIVVYRHNAKSTHKRKLGHRQPFTRVKITEIKEK", "aa_hexdigest": "2e1b8b13d765a237bc4b0b6090d9d3bf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223A", "ncbi_nrp_id": "WP_012855541.1", "uniref100_id": "UniRef100_D1J853", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223A", "RefSeq:WP_012855541.1", "UniRef:UniRef100_D1J853" ] }, "ips": { "uniref100_id": "UniRef100_D1J853", "uniref90_id": "UniRef90_D1J853" }, "psc": { "uniref90_id": "UniRef90_D1J853", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_Q6F123", "cog_id": "COG0261", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F123", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F123" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "LMJBGEANGM_619", "locus": "GCA013368015_00604" }, { "type": "cds", "sequence": "contig_1", "start": 343339, "stop": 343614, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAGATTACATTAAAATGGTTAACAATTTTAGGGGCAACAACGCTTACTGCTTTGCCTTTTTGTGCAATTTCTTGTAGTAATGAGAAAAAAGAAATCGAAAAAGCAGAGAAAATTATTGGCGATAAAGAACTAACATTTAAAATAGATGATTTAAAATATGAAAATTCTGATAATTCTATATTGCAAAGTGCTAGAAGTATTGCAGGACCATGGCGCAAGGACTTTCTTAAGGTTGTTATAACGTCAAAATCAAATATTGAAGAATATTGA", "aa": "MKKITLKWLTILGATTLTALPFCAISCSNEKKEIEKAEKIIGDKELTFKIDDLKYENSDNSILQSARSIAGPWRKDFLKVVITSKSNIEEY", "aa_hexdigest": "dc9da4fa6d83d0f0490d9277a0a9ac1c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10324.895999999992, "isoelectric_point": 8.545107078552245 }, "id": "LMJBGEANGM_620", "locus": "GCA013368015_00605" }, { "type": "cds", "sequence": "contig_1", "start": 343720, "stop": 344043, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U0", "UniRef:UniRef90_A0A454C9U0" ], "nt": "ATGGATCTAAAATCTTCTTACCAAAAAGCAAACAAAGCATATTTAATAGAAAAACCAAAAAAAGTATATTCAAATCTAAAAAATAAACAAATATTAATAAATAACTACACTATATTTTTTGATGATATAAATAATGATAAATCTCCAAGAGTTTCAATGGCGATAAAAGAATTAAAATTTAATTTTGATCAAGACCGAAAAACCATAGAAATTAAACTTGGTTTAAAATATGAATTATCACAAAAACATAATAGGAAGTCTAATATATTTTTTCTTTCTCCTAAAGCAACCTTTAAATACATTTTAAGCGAAGAAATCAAATAA", "aa": "MDLKSSYQKANKAYLIEKPKKVYSNLKNKQILINNYTIFFDDINNDKSPRVSMAIKELKFNFDQDRKTIEIKLGLKYELSQKHNRKSNIFFLSPKATFKYILSEEIK", "aa_hexdigest": "2ce85dc901cf472134bd676356d45b5c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 343420, "stop": 344043, "observations": { "mutation": [ 343612, 343624 ], "stop": [ 343612, 343624 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U0", "query_cov": 1.0, "subject_cov": 0.45726495726495725, "identity": 0.972, "score": 200.0, "evalue": 2.72e-63, "gene_start": 1, "gene_end": 107, "reference_start": 128, "reference_end": 234, "reference_sequence": "MKKITLKWLTILGATTLTALPFCAISCSNEEKEIEKAEKIIGDKELTFKIDDLKYENSDNSILQSARSIAGPWRKDFLKVVITSKSNIEEYWKDNWIVGKIDLKNKAILFLIPREVLLDADFIKEKNMDLKSSYQKANKAYLIEKPKKVYSNLKNKQIWINNYTIFFDDINNDKSPRVSMAIKELKFNFDQDQKTIEIKLGLRYELSQKHNRKSNIFFLSPKATFKYILSEEIK" }, "paralog": false, "identity": 0.9710144927536232, "score": 392.0, "evalue": 1.79e-141, "description": "Internal stop codon at 343612, 343624. Nonsense mutation around 343612, 343624.", "psc": { "uniref90_id": "UniRef90_A0A454C9U0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U0", "UniRef:UniRef50_A0A454C9U0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U0" ], "product": "Lipoprotein" } }, "id": "LMJBGEANGM_621", "locus": "GCA013368015_00606" }, { "type": "cds", "sequence": "contig_1", "start": 344237, "stop": 344410, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_155392267.1", "SO:0001217", "UniParc:UPI000A4BC0B6", "UniRef:UniRef100_UPI000A4BC0B6", "UniRef:UniRef50_A0A6A8PZY9", "UniRef:UniRef90_A0A6A8PZY9" ], "nt": "ATGCAGCAAGAGGCTAAATTAAACAACGATTATTCAAAGCTCTTTAATTCTTGTCAAAACCAAATTAAGAATTTAAATAATTTAAACATCGAAAATAAATTTCTAAAACAAAGAATTTTAAAAATTATAAATAATGAAATAAATGATTTAAAAAACAAGTTGGTAATAAGATAA", "aa": "MQQEAKLNNDYSKLFNSCQNQIKNLNNLNIENKFLKQRILKIINNEINDLKNKLVIR", "aa_hexdigest": "2ee4ac2c580e98e2b7afa3988cf60ef7", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000A4BC0B6", "ncbi_nrp_id": "WP_155392267.1", "uniref100_id": "UniRef100_UPI000A4BC0B6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000A4BC0B6", "RefSeq:WP_155392267.1", "UniRef:UniRef100_UPI000A4BC0B6" ] }, "ips": { "uniref100_id": "UniRef100_UPI000A4BC0B6", "uniref90_id": "UniRef90_A0A6A8PZY9" }, "psc": { "uniref90_id": "UniRef90_A0A6A8PZY9", "uniref50_id": "UniRef50_A0A6A8PZY9" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZY9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6830.848899999999, "isoelectric_point": 9.730745124816895 }, "id": "LMJBGEANGM_622", "locus": "GCA013368015_00607" }, { "type": "cds", "sequence": "contig_1", "start": 344597, "stop": 345226, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAATTCAAAGAATTTATCGTATTCAAGGTATTGCAATTAGCGATAAGTATATTGAAATTATAATTCGTCAAATGATGTCTAAATTAGTAATTTCTGAACCCGGAGATTCTAAATTCTTCGCTGGTGCTATTGTAGATATTTTTGACTATCAAGAAGAAAATGCTATTCTACTTTCAAAAGGTTTGAAGCCAGCATATGGTAAAGTTGTTATTAAAGGTGCAAAACAAGTTCCATTGTTATCTGATTCATTCCTTGCCGCTGCTTCTTATCAAGAAACAAGCAAGATATTAGTTCACGCTGCTATTTCATCAAGAATTGACAATTTATCTGGCTTAAAAGAAAATATTATTCTTGGTAAAAAGATTCCATCAGGAACAGCATTATTCCCATTTGAAGAACATTCAAAATATGATATAAAACCATCAATTAATTACTTTACAATAACTCCTATTGACGGCACAGAAATTTCTGAACCTATTGATATTGATTCAGTTGTTAATACAGCGCAAGAATATAACAATGATAAACAATCAGTTGAAGAAGATTATTCTCCAAACGACGAAGAACTTGTTGCTGAAGAAGAAACATTTGATGATGAAGAAAATCAAAACGATTTTGAATAA", "aa": "MKEIQRIYRIQGIAISDKYIEIIIRQMMSKLVISEPGDSKFFAGAIVDIFDYQEENAILLSKGLKPAYGKVVIKGAKQVPLLSDSFLAAASYQETSKILVHAAISSRIDNLSGLKENIILGKKIPSGTALFPFEEHSKYDIKPSINYFTITPIDGTEISEPIDIDSVVNTAQEYNNDKQSVEEDYSPNDEELVAEEETFDDEENQNDFE", "aa_hexdigest": "75bf2e5330dce59fad9d436994480378", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23450.050099999997, "isoelectric_point": 4.457791328430175 }, "id": "LMJBGEANGM_623", "locus": "GCA013368015_00608" }, { "type": "cds", "sequence": "contig_1", "start": 345494, "stop": 346255, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGATTAACTCAGGTGCTCGTGGTAACATTTCTAACTATGTTCAATTAGCAGGTATGCGTGGATTGATGGCTAACAATACTAAAACTACTAAGGCCGATGCTAAAAACGACCGTGTTGTTAGATCAACAGTTGAAGTTCCTGTTAAGTCTTCATTTATTGAAGGACTTACTGCATTTGAATTCTATTCATCAACTCACGGTGCTAGAAAAGGTTTAACCGATACTGCCTTGAACACTGCAAAATCTGGTTATTTAACAAGACGTTTAGTTGATGTTGCTCAAAATATTGTTGTTAGAGAAGAAAACTGTGGCACTGACTATGGTTATAAAGCAAGAAACATAGTTGATACAAAAACTAAACAAATTATTGTTCCTTTGAAAGAAAGAATTGTTGGAAGATTTGCAAACAAACCTGTTGTTACAAGCGCAGGTATAGAAATTTGTTCTAGAAATGAATTAATTACTGAAAAAATTGCTCAACGAATAATAGCAAGTGGTATTGAAGAAGTAGAAATTAGATCAATCTTAGGCTGCAATACACGTAACGGTGTATGTAAGATGTGTTTTGGTAAAGACCTTGCAACAAATAGAATTGTTAATATCGGTGAAGCAGTAGGTATTATTGCAGCTCAATCTATTGGGGAACCAGGAACTCAATTAACTATGCGTACATTCCACACTGGTGGTGTTGCCGGGGTTGAAGATATTACTGGTGGTTTCACTAGATTGATCGAATTAATTGATGCCCACGAACAACCATGA", "aa": "MINSGARGNISNYVQLAGMRGLMANNTKTTKADAKNDRVVRSTVEVPVKSSFIEGLTAFEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQNIVVREENCGTDYGYKARNIVDTKTKQIIVPLKERIVGRFANKPVVTSAGIEICSRNELITEKIAQRIIASGIEEVEIRSILGCNTRNGVCKMCFGKDLATNRIVNIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFTRLIELIDAHEQP", "aa_hexdigest": "0d69966ffc331c42d09c365b161e2ab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 56, "aa_cov": 0.20948616600790515, "hmm_cov": 0.9821428571428571, "evalue": 5.856546010130441e-12, "score": 41.05525588989258, "start": 1, "stop": 53 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.7707509881422925, "hmm_cov": 0.6, "evalue": 5.949595423012786e-47, "score": 155.84100341796875, "start": 55, "stop": 249 } ], "seq_stats": { "molecular_weight": 27363.022100000013, "isoelectric_point": 9.110559272766114 }, "id": "LMJBGEANGM_624", "locus": "GCA013368015_00609" }, { "type": "cds", "sequence": "contig_1", "start": 346373, "stop": 346528, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAGTATTAAACAATTAGGTTTTGACTATTCAACATATTCTGGAATTTCTATTTCGTTTAGTGATATTCTAGAAACTAATAAAAAGCAAGAATATATTGCTCAAGGTGATGAATATATTGCTAAATTAAAACACTACTATGAACAAGGTTGA", "aa": "MDSIKQLGFDYSTYSGISISFSDILETNKKQEYIAQGDEYIAKLKHYYEQG", "aa_hexdigest": "fd1d13a9a161c1bc127e024eb4652b75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5917.4749, "isoelectric_point": 4.749659538269043 }, "id": "LMJBGEANGM_625", "locus": "GCA013368015_00610" }, { "type": "cds", "sequence": "contig_1", "start": 346559, "stop": 347503, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00623.25", "PFAM:PF04983.23" ], "nt": "TTGTTAAACCGTGCACCTACATTACACCGTTTATCTATTCAAGCCTTTGAACCTGTTTTAGTAAGAGGTAAGGCTATTAGATTACACCCATTAGTATGTACTCCATTTAATGCTGACTTCGATGGTGACCAAATGGCTGTTCACGTTCCTGTTTCTGAACAAGCATTGCTTGAAAGTCGTGAATTAATGTTAGCTAATAAAAACATTCTAGGACCTAAAGATGGTGAACCTATTATTAACCCTTCTCAAGATATGGTTTTAGGTATTTATTATCTAACTATTGAAGAACCAGGGGCTAAAGGTGAAGGACGTGTTTTTGATAACTATGAACACATGCTTAGAGCATTAGAAAATAAAGTAGTTACATTGCACACTAGAATAGCATTACCTGCTTCAGAAGTTAAAACTCCAAAACTTATTTTCCCATTTGATGGTCCTCAATATATAATTTCAACAGTAGGTAAATTTATTTTTAATAATATATTTCCTAAAACATTTCCATTTATTTACGACAATAAAGTTGCTAAGGCAAATTCATTGGAAGATTATAATCAAACATTCAATGCAAAATATGTCGTTCAAGCAGGTACTAATATTCCTGAATATATCAAAAATATGCCTATTGAAGAAGCATTTAACAAAAAGAATATTTCTAAAATAATACGTTATGTTTTTGATAAATATGTTGCTCAAATTTCAATGTCTGATATTGCAGAAGTTATTAATAATTTAACTGAATCAAGTGATGCTAATGATTCAATAATTCAATTCTTGCATGTTAAAAATTATGAAAATAGAAAAATTGATTATGAACATGCAATGTTGCTAGCTCAATTTACAAGTCAAGAAAAGCAAAAGATCGAAATGTTAAATCCTGCTAGGGCAAATGGACAAGAAGTTCCACTTAGTCCAGCTGAAAAAGCAGAAATTCTAGACACTGTATGA", "aa": "MLNRAPTLHRLSIQAFEPVLVRGKAIRLHPLVCTPFNADFDGDQMAVHVPVSEQALLESRELMLANKNILGPKDGEPIINPSQDMVLGIYYLTIEEPGAKGEGRVFDNYEHMLRALENKVVTLHTRIALPASEVKTPKLIFPFDGPQYIISTVGKFIFNNIFPKTFPFIYDNKVAKANSLEDYNQTFNAKYVVQAGTNIPEYIKNMPIEEAFNKKNISKIIRYVFDKYVAQISMSDIAEVINNLTESSDANDSIIQFLHVKNYENRKIDYEHAMLLAQFTSQEKQKIEMLNPARANGQEVPLSPAEKAEILDTV", "aa_hexdigest": "8f0519f041463e03258216f443648e55", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00623.25", "name": "RNA_pol_Rpb1_2", "length": 64, "aa_cov": 0.20382165605095542, "hmm_cov": 1.0, "evalue": 1.4894060652720918e-26, "score": 89.17303466796875, "start": 1, "stop": 64 }, { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 182, "aa_cov": 0.5605095541401274, "hmm_cov": 0.8131868131868132, "evalue": 3.7338409586483775e-26, "score": 87.4749755859375, "start": 68, "stop": 243 } ], "seq_stats": { "molecular_weight": 35558.41750000004, "isoelectric_point": 5.721265220642091 }, "id": "LMJBGEANGM_626", "locus": "GCA013368015_00611" }, { "type": "cds", "sequence": "contig_1", "start": 347627, "stop": 347902, "strand": "-", "frame": 2, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "TTGGTTATTCAAAACGAACTAAGAATGATTCAAGAAGCAGTAGATGCTTTAATTGATAACCAAAGAAGAACACCTAACCCTGTTTTATCAAAAGACAATCGTCCATTTAAATCAATTTCAGATGCATTAACTGGTAAAAAAGGTCGTTTTAGACAAAATTTACTAGGAAAACGTGTTGACTATTCTGGACGTTCAGTTATTGCCGTAGGACCTTCATTAAAAATGCACCAATGTGGTATACCAAGAGAAATGGCTGCTAAATTATTTGAACCATGA", "aa": "MVIQNELRMIQEAVDALIDNQRRTPNPVLSKDNRPFKSISDALTGKKGRFRQNLLGKRVDYSGRSVIAVGPSLKMHQCGIPREMAAKLFEP", "aa_hexdigest": "4958223e7fd21991b5516508c96ea886", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002381C6D8", "query_cov": 0.9340659340659341, "subject_cov": 1.0, "identity": 0.624, "score": 112.0, "evalue": 1.02e-30, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "LMJBGEANGM_627", "locus": "GCA013368015_00612" }, { "type": "cds", "sequence": "contig_1", "start": 347924, "stop": 348610, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "TTGCTTGGTTTAAAATCTTCAAATGGTTCTGACGTTGTTAAACGTGAAGAACTAGAAAATTTAATTTATTACAAAAATCATATTGTTCTAGAAGATGGTGGCTTAAAATCATTACCTAAAAATCAAATTATTGAAATCAATGAAGCGGCAATCATTTACCGCAATGCTCTAGAAGAATTGCTTGAAAGATTTCAAGAAGGTACAGAAGAATATGATGATATTAAAGAAACCCTAGATTCTTTAATTGAAAAAGCAACATCAAAAATCGGTCAAGAATATGGAATTGATTTTTATGAATTAAACGAAGTAATTGAACATTATTCAGACGCTAAAATAGGTACTGGTGCTTTAGCTATTGAATATTTATTAAAAAACATAAATTTAGCTGAAGAAAAGCAAAAAGTAACTGATGAAATTAATCGTTTAAATGCAGAAGAAATTAATAATCCTGAACGTTTTGCCACAACTGCAAAACAAACAAGAGAAAAACTTTATAAACGTTTACAAGTAATTAATGCATTTATTGAATCTAAGCAAGATCCAACTAGCATGTTAATTTATAATTTGCCTGTTATTCCAGCAGATTTAAGACCTTTAATTCAATTAGATGGTGGTAGACACTCAACTAGTGATATTAATGAATTGTATCGTAGAGTAATTATTAGAAACAATAGATTGCAACAATGA", "aa": "MLGLKSSNGSDVVKREELENLIYYKNHIVLEDGGLKSLPKNQIIEINEAAIIYRNALEELLERFQEGTEEYDDIKETLDSLIEKATSKIGQEYGIDFYELNEVIEHYSDAKIGTGALAIEYLLKNINLAEEKQKVTDEINRLNAEEINNPERFATTAKQTREKLYKRLQVINAFIESKQDPTSMLIYNLPVIPADLRPLIQLDGGRHSTSDINELYRRVIIRNNRLQQ", "aa_hexdigest": "c57cfefce064fd45e8f993c30529a07d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 222, "aa_cov": 0.9342105263157895, "hmm_cov": 0.7207207207207207, "evalue": 1.2585640101808774e-22, "score": 76.0958480834961, "start": 16, "stop": 228 } ], "seq_stats": { "molecular_weight": 26268.37680000003, "isoelectric_point": 4.939615440368651 }, "id": "LMJBGEANGM_628", "locus": "GCA013368015_00613" }, { "type": "cds", "sequence": "contig_1", "start": 348644, "stop": 349003, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGAAAAAAATTTCAAAATACGAATCATTAGATGAAGAAAAAATTTCAAAAATTTCATTAGCATTAGCAACTCCTCAAGATGTTGAAACTTGGTCTCATGGTGAAGTTACTAAGCCAGAAACTATTAACTATAAATCATATAAGCCAGAACGTGGTGGATTATTTGATGAAATAATTTTTGGACCAATGATTGATTATCGTTGCCCTGTATGTGGTTTCAAATACAAAAAAGTTAATGAAGGAAGCTATTGTACAAGAACTGAATTATGTAAACAAGAACATGTTCAAATATTGCCTAAAATAGCACGTAGAACCCATATGGGACACATAAAACTAAATAGTCCAGTTGTTCACTTTTGA", "aa": "MKKISKYESLDEEKISKISLALATPQDVETWSHGEVTKPETINYKSYKPERGGLFDEIIFGPMIDYRCPVCGFKYKKVNEGSYCTRTELCKQEHVQILPKIARRTHMGHIKLNSPVVHF", "aa_hexdigest": "7ab28ebbdd5fde8231c32dc012a94f7b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 109, "aa_cov": 0.8991596638655462, "hmm_cov": 0.7522935779816514, "evalue": 5.5863730285744904e-24, "score": 80.53942108154297, "start": 13, "stop": 119 } ], "seq_stats": { "molecular_weight": 13760.796499999986, "isoelectric_point": 8.857262992858885 }, "id": "LMJBGEANGM_629", "locus": "GCA013368015_00614" }, { "type": "cds", "sequence": "contig_1", "start": 349005, "stop": 349175, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAACCAGGAACACCTGAATCGTTTAATGTTTTAGCATATGAATTACGTGGTTTAGGAATTAAATTAGAAGTACATCAAAAATCTGATAATGTTGTAGATGATTCAGAAGAAGATCAAAGAGTAGACACAAAATATTTCCAAGAATTTGAAGGGAGTTTAGAATAA", "aa": "MPKPGTPESFNVLAYELRGLGIKLEVHQKSDNVVDDSEEDQRVDTKYFQEFEGSLE", "aa_hexdigest": "b5604bd1c3019299f2f7444a7ee5c308", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6415.9671, "isoelectric_point": 4.457734489440917 }, "id": "LMJBGEANGM_630", "locus": "GCA013368015_00615" }, { "type": "cds", "sequence": "contig_1", "start": 349287, "stop": 350189, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "TTGGTTAAAGATGATGTTTATACATCAATTCACATTGAAGAACAATCAATTCAATTTAGACATTCAAAGGCTGGCGAAGATATTTTAACTGCAGATATTCCTAACGTTTCAAACTATTCTAAACGTTTCTTAGGTGAAAATGGAATTGTTAGAGTTGGTTCAGAAGTAACAGCAGGTGATATTTTAGTCGGAAGAACTTCTCCAAAAGGTGAAGAAAACCCAACTCCAGAAGAAAAATTAATGGCTGCAATTTTTGGTCAAAAAACTGCCTCAAGAAAAGATACTTCTCTAAAAGTAAAACATGGCCACAATGGAACCGTTGTTGCTGTTGATATTCTAAGTAGAGAATTTGGCGACCAATTAGAAGATGGCATTGAAAAAATAGTTAAGGTATCAATTGCTCAAAAACGTAAAATTCAAGTCGGCGACAAGATGGCTGGTCGTCACGGAAATAAAGGTGTTGTTTCAATAGTATTGCCTGTTGAAGAAATGCCTTATTTAGCAGATGGAACACCATTAGATATTGTTTTAAACCCTCAAGGTGTCCCTTCACGTATGAACATTGGTCAAGTTCTAGAATTGCACTTAGGACTTGCTGCTAAAAAATTAAATACCAAATTTGTTACACCAATATTTGATGGAATTTCACACAACCAAATTCGTGATATTCTTGAAGAAGCAAATATTGATGTAACAGGAAAATCAATAGTATACGATGGGGCTACCGGAGAACCATTTGATCATCCAATTTCTGTAGGGATTATGTACTATTTGAAGTTGTATCACATGGTTGATGACAAAATGCACGCACGTTCAGTAGGACCTTATTCATTGATTACCCAACAACCACTTGGTGGAAAGAGTCAAAATGGTGGTCAAAGATTTGGTGAAATGGAAACATGA", "aa": "MVKDDVYTSIHIEEQSIQFRHSKAGEDILTADIPNVSNYSKRFLGENGIVRVGSEVTAGDILVGRTSPKGEENPTPEEKLMAAIFGQKTASRKDTSLKVKHGHNGTVVAVDILSREFGDQLEDGIEKIVKVSIAQKRKIQVGDKMAGRHGNKGVVSIVLPVEEMPYLADGTPLDIVLNPQGVPSRMNIGQVLELHLGLAAKKLNTKFVTPIFDGISHNQIRDILEEANIDVTGKSIVYDGATGEPFDHPISVGIMYYLKLYHMVDDKMHARSVGPYSLITQQPLGGKSQNGGQRFGEMET", "aa_hexdigest": "a56dec150c290f662ff4229d0c0ca721", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0G1DPB0", "query_cov": 0.9666666666666667, "subject_cov": 0.8011049723756906, "identity": 0.5479999999999999, "score": 333.0, "evalue": 1.42e-110, "valid": false, "product": "DNA-directed RNA polymerase", "uniref50_id": "UniRef50_A0A660M9J4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 291, "aa_cov": 0.96, "hmm_cov": 0.9587628865979382, "evalue": 1.3910155271222075e-104, "score": 345.6925964355469, "start": 2, "stop": 289 } ], "seq_stats": { "molecular_weight": 32798.00410000002, "isoelectric_point": 6.073553276062011 }, "id": "LMJBGEANGM_631", "locus": "GCA013368015_00616" }, { "type": "cds", "sequence": "contig_1", "start": 350232, "stop": 351437, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04565.21", "PFAM:PF10385.14", "PFAM:PF00562.33" ], "nt": "ATGTTGCATGACCAAAAAGTTTTGGTTTTAGGTAATGATCCTACATCAACAGAAAATTTCTTGTTGATTCCTGATTTAATTGCAACGATTTCGTATTATTTTAATTTACTTTCAAAGGTTGGGGTTGATGATGATCCTGACTCATTAGTTAATAAAAGAATTGTAACAATAGGCGAATTATTACAAAATCAATTTAGAATAGGGCTATTAAAATTAGAAAAAAATACAAGAGAAAGAATCTCAGCAAAAGATATTGATAAGATTACTGCTAAGAACGTTACAAATAATAAACCAATATTTAATCAATTTAAATCGTTCTTTAACACATCAAAACTTTCTCAATTTATGGATCAATGTAATCCTCTTGCTGAAATATCAAACAAGAGAAGAATAACCTCCCTAGGACCAAGAGGGTTGAACCGTGATACTGCACAATTTGAAGTTCGTGACGTTCACCCTACCCATTATGGAAGAATTTGTCCTATTGAAACACCCGAAGGACCAAACATTGGTTTAATTTTAAATTTAGCATCATTTGCTAAAATTGATAAATATGGTTTTATTCAATCACCATATTTCAGAGTAAATAAAAAAGTTGTAGATTTTTCAGAACCAGTTTACATTACTGCAATTGAAGAAACAGGATATACATTTGCACAATCAACTGTTCACATTGAAGGTAATGTAATTGTTGATGAAAAAGTAATGGTAAGACGTGACAATGAATATTTAGAAGTAAATGCTGAAGATGTAGATTTTATTGGTGTCTCAAACCGTCAAATGACCTCAATAGCTGCTTCGGCTATACCATTTTTGGAAAACGATGATGCTAACCGTGCATTGATGGGTTCAAACATGCAACGTCAAGCTGTTCCAGTGTTATTCCCAGAAGCACCATTAGTTGCTACAGGAGTTGAAGCTGATATTGCTAAATATTCATCAACCAATATTAGAGCAACCAGATCAGGTGTTGTTAACTATGTAGATGCTAACGTTATTAAAGTAACTCCTGAAAATAGTTCAAAAAGTGATGTTTATCATTTAAGAACATTTGAAAGAAGTAACCAAGGTACATTAATTCACCAAGTACCTTTGGTTAAAGTTGGCCAACAAATTAATGCGGGAGATTTATTAGTTGATGGTCCTTCGATGAAAGATGGAGAATTAGCATTAGGAAAAAATGTTCTAGTAGGTTTTACTACTTGA", "aa": "MLHDQKVLVLGNDPTSTENFLLIPDLIATISYYFNLLSKVGVDDDPDSLVNKRIVTIGELLQNQFRIGLLKLEKNTRERISAKDIDKITAKNVTNNKPIFNQFKSFFNTSKLSQFMDQCNPLAEISNKRRITSLGPRGLNRDTAQFEVRDVHPTHYGRICPIETPEGPNIGLILNLASFAKIDKYGFIQSPYFRVNKKVVDFSEPVYITAIEETGYTFAQSTVHIEGNVIVDEKVMVRRDNEYLEVNAEDVDFIGVSNRQMTSIAASAIPFLENDDANRALMGSNMQRQAVPVLFPEAPLVATGVEADIAKYSSTNIRATRSGVVNYVDANVIKVTPENSSKSDVYHLRTFERSNQGTLIHQVPLVKVGQQINAGDLLVDGPSMKDGELALGKNVLVGFTT", "aa_hexdigest": "a5684565194a5de4b4ea6da7d1130d75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04565.21", "name": "RNA_pol_Rpb2_3", "length": 69, "aa_cov": 0.17206982543640897, "hmm_cov": 0.9855072463768116, "evalue": 7.582350200438765e-30, "score": 98.50003814697266, "start": 114, "stop": 182 }, { "id": "PF10385.14", "name": "RNA_pol_Rpb2_45", "length": 67, "aa_cov": 0.16458852867830423, "hmm_cov": 0.9850746268656716, "evalue": 2.2898929175450914e-15, "score": 51.9917106628418, "start": 192, "stop": 257 }, { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 86, "aa_cov": 0.20448877805486285, "hmm_cov": 0.8837209302325582, "evalue": 1.9687419492575112e-10, "score": 35.854862213134766, "start": 319, "stop": 400 } ], "seq_stats": { "molecular_weight": 44613.200400000074, "isoelectric_point": 5.792484474182129 }, "id": "LMJBGEANGM_632", "locus": "GCA013368015_00617" }, { "type": "cds", "sequence": "contig_1", "start": 351609, "stop": 351935, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19" ], "nt": "ATGTTTGGCGAAAATATTCCAGAATTGGAAGAAACATTTAAACGTGATAAAATCACTGGAATTAATGCAGAAGAAGTTACAAGAGAAGCACAAGAAGCAATTTATAGAATCATCAGAAAAGGCGATCGTATGACAGCTGATTCTGCTAGAAATTTAATTCCTACAACTTTGTTTAATGAAAAAAGATATAGTTTAACTGAAACAGGACGTTTCACTTTAAATAGAAAATTAAACATTATTGAAAGAATTGCTAATTCTTATTTAGCAGAAGATATTTTTTCTAGTGAAGGTGAACTTCTTTATGAAAAAGGAATGTTCATTACTTGA", "aa": "MFGENIPELEETFKRDKITGINAEEVTREAQEAIYRIIRKGDRMTADSARNLIPTTLFNEKRYSLTETGRFTLNRKLNIIERIANSYLAEDIFSSEGELLYEKGMFIT", "aa_hexdigest": "381928d4fce804669575b1b026c59d28", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 71, "aa_cov": 0.6296296296296297, "hmm_cov": 0.9154929577464789, "evalue": 2.9702463591129206e-06, "score": 22.57516098022461, "start": 27, "stop": 94 } ], "seq_stats": { "molecular_weight": 12558.086299999986, "isoelectric_point": 5.114622688293457 }, "id": "LMJBGEANGM_633", "locus": "GCA013368015_00618" }, { "type": "cds", "sequence": "contig_1", "start": 352065, "stop": 352604, "strand": "-", "frame": 1, "gene": null, "product": "RNA polymerase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54", "UniRef:UniRef90_UPI002FDFDE54" ], "nt": "ATGGTGAATAAGGATAAATACGAAATTAGAAAATTTGGACCTATTACCCTAAGACGTGACTATTCAATTAGTCAAAAGAAATTCGAAACTCCTGACTTTCTTGAAATGCAACGTGAATCAGTTGAAAAATTTCTAACAGTTGGAATTGAAGAAGAATTAAGAAATATCTATCCAATTGAAGCACATGGAAAAGTTAGAATTGAATACATTCATAATTCAGCACATTTTGAATATCCTAAAAAAACTGAATATGAAAGCATTAAAGAAGCTAAACAAAAAGGTTCATCATATCAAGGAAAGTTAAAAGCACACCTAAGACAAATCAATATCGAAACAGGTGAAGTTGAAGATTCAGAAGTAGTATTTGCTGAAATTCCTATAATGACTTATGGTGGATCATTTATTATCAATGGTTCAGAAAAAGTTATTGTTTCGCAATTAATTAGATCTACCGGGGCTTATTTTGGAATTAATGTTAGAAATAAACAAGCCAACGACTTGTTTAATAAAGTAGAAATTATTCCTCAAATTGGTTCATGA", "aa": "MVNKDKYEIRKFGPITLRRDYSISQKKFETPDFLEMQRESVEKFLTVGIEEELRNIYPIEAHGKVRIEYIHNSAHFEYPKKTEYESIKEAKQKGSSYQGKLKAHLRQINIETGEVEDSEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGINVRNKQANDLFNKVEIIPQIGS", "aa_hexdigest": "73e8e121e79577765c555dbc7556d4bc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 351762, "stop": 352595, "observations": { "mutation": [ 352067 ], "stop": [ 352067 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDFDE54", "query_cov": 0.9832402234636871, "subject_cov": 0.5482866043613707, "identity": 0.8859999999999999, "score": 307.0, "evalue": 4.96e-103, "gene_start": 4, "gene_end": 179, "reference_start": 3, "reference_end": 178, "reference_sequence": "MVKAKYELRKFGPITERRDYSISQKKFATPDFLEMQRTSVENFIHQGIEEELRNIYPIESHGKVRIEYVWNSAHFDYPKKSEFESIKEAKQKGSSYQGKLKARLRQINLETGEVEESEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGLNVRNKQANDLFNKVEIIPQVGSWVEIYHKVTSANADSVKIHIDKNKSFLLTTFLKALGFSETGIRAMFGDIAELEETLKKDKIVGLSLEESTREAQEAIYRIIRKGDRMTVESARNLIPSTLFNEKRYNLTQIGRFTLNRKLNVIERIANSFLAESIFSDKGELL" }, "paralog": false, "identity": 0.851985559566787, "score": 465.0, "evalue": 1.11e-167, "description": "Internal stop codon at 352067. Nonsense mutation around 352067.", "psc": { "uniref90_id": "UniRef90_UPI002FDFDE54", "product": "RNA polymerase beta subunit", "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDFDE54", "UniRef:UniRef50_UPI002FDFDE54" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54" ] } }, "id": "LMJBGEANGM_634", "locus": "GCA013368015_00619" }, { "type": "cds", "sequence": "contig_1", "start": 352738, "stop": 353112, "strand": "-", "frame": 3, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_012855544.1", "SO:0001217", "UniParc:UPI00000BF3D8", "UniRef:UniRef100_D1J856", "UniRef:UniRef50_B3PM75", "UniRef:UniRef90_B3PM75" ], "nt": "ATGGCAAAATTAACAAAAGAAGAATTTGTTTCAGCATTAAAAGAAATGAACATTAAAGAAGTTATGGAATTAATTCAAGGATTAAAAGAAGAATTTGGAATTGATCCAACAGCAGTTGTAGCAGCAGCAGCTCCAGCAGCAGCAGCTGACGCAGCAGAAGAAAAGACTGTATTTAATGTTACTTTAAAATCAGATGGTGGAAACAAGCTAGCAGTTATTAAAGTTGTTAAAGACTTATTAGGTCTAGGATTAATGGATGCTAAAAAACTTGTTGAATCAGCACCTGTTGTAATTAAAGAAAATGCTAAAAAAGAAGAAGCTGAAGAACTAAAAGCTAAATTAACAGAAGCTAAAGCCGAAGTTACTTTAGACTAA", "aa": "MAKLTKEEFVSALKEMNIKEVMELIQGLKEEFGIDPTAVVAAAAPAAAADAAEEKTVFNVTLKSDGGNKLAVIKVVKDLLGLGLMDAKKLVESAPVVIKENAKKEEAEELKAKLTEAKAEVTLD", "aa_hexdigest": "53b91ce3f730c59f29e8ab79a1e81e35", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000BF3D8", "ncbi_nrp_id": "WP_012855544.1", "uniref100_id": "UniRef100_D1J856", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BF3D8", "RefSeq:WP_012855544.1", "UniRef:UniRef100_D1J856" ] }, "ips": { "uniref100_id": "UniRef100_D1J856", "uniref90_id": "UniRef90_B3PM75" }, "psc": { "uniref90_id": "UniRef90_B3PM75", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_B3PM75", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM75" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "LMJBGEANGM_635", "locus": "GCA013368015_00620" }, { "type": "cds", "sequence": "contig_1", "start": 353135, "stop": 353632, "strand": "-", "frame": 2, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02864", "RefSeq:WP_012855545.1", "SO:0001217", "UniParc:UPI0001BC223D", "UniRef:UniRef100_D1J857", "UniRef:UniRef50_A0A448ZYM3", "UniRef:UniRef90_D1J857" ], "nt": "ATGAGCGCATTAAAAGATGCCAAGGTTTTAGTTGTTGAAGAAATTTCAAACAATTTAAAAGAATCACAAGCATTATATGTAATTAATTATGCTACTTTGGATGTTGTTTCATTTCAAGAAATTAGAAAAGAATTAGCTCCAAATAATGCATTGCTAAAAGTTTACAAAAATAGACTAGTTAAACAAGCATTAAAAAATACAGAATATGCAAATATTAATGATAGTTTAGTTTTACAAAATGCTTATGCCTTTGTTAAAGGCGATTCGTTAGCAGCATTAAAGACCTTAGTAGGCTTAAAGAAGAAATTCCCTGCATTAAGAATTGTAAGTGGAATTTATGAAAATAAAGTAGTTGATGAAAAAACATTAGACGAAATATCAAAACTTCCTTCATTTACAGAATCACTTATGATTCTTGGAAATTCATTATTGAGTCCATTGAAACAATTATCAATTGGTTTAAATGAATTAATAAAACAAGGAAAAATTTCAGAATAA", "aa": "MSALKDAKVLVVEEISNNLKESQALYVINYATLDVVSFQEIRKELAPNNALLKVYKNRLVKQALKNTEYANINDSLVLQNAYAFVKGDSLAALKTLVGLKKKFPALRIVSGIYENKVVDEKTLDEISKLPSFTESLMILGNSLLSPLKQLSIGLNELIKQGKISE", "aa_hexdigest": "4e952e6b06822b7a4ca63497eef65389", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223D", "ncbi_nrp_id": "WP_012855545.1", "uniref100_id": "UniRef100_D1J857", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223D", "RefSeq:WP_012855545.1", "UniRef:UniRef100_D1J857" ] }, "ips": { "uniref100_id": "UniRef100_D1J857", "uniref90_id": "UniRef90_D1J857" }, "psc": { "uniref90_id": "UniRef90_D1J857", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_A0A448ZYM3", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYM3" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "LMJBGEANGM_636", "locus": "GCA013368015_00621" }, { "type": "rRNA", "sequence": "contig_1", "start": 353852, "stop": 356748, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9904273504273504, "score": 2760.4, "evalue": 5e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTGCAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGACGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTTATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "LMJBGEANGM_40", "locus": "GCA013368015_00622" }, { "type": "rRNA", "sequence": "contig_1", "start": 356933, "stop": 358450, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1512.0, "evalue": 2.7e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGGACCGCATGGTTCCGTTGTGAAAGGCGCTGTAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "LMJBGEANGM_36", "locus": "GCA013368015_00623" }, { "type": "cds", "sequence": "contig_1", "start": 358849, "stop": 359046, "strand": "-", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855546.1", "SO:0001217", "UniParc:UPI0000DB8019", "UniRef:UniRef100_D1J858", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_D1J858" ], "nt": "ATGGCAGTAGTACCAAAAAGAAAAACTTCAAAACAAAGAAAACATTTAAGAAGATCACATCACGCTCTAGTGGCTCCAACATTAGTTGAATGTTCACAATGTAAGAATTTAATTACTCCTCACCAAGCATGTGAAAACTGCGGTTTTTATAGAGGAAGAAAAGTTATTAAAGAAGCAATTAACGACAAAATTAAATAA", "aa": "MAVVPKRKTSKQRKHLRRSHHALVAPTLVECSQCKNLITPHQACENCGFYRGRKVIKEAINDKIK", "aa_hexdigest": "2966d01971762bee811933c45cb0dcc6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000DB8019", "ncbi_nrp_id": "WP_012855546.1", "uniref100_id": "UniRef100_D1J858", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000DB8019", "RefSeq:WP_012855546.1", "UniRef:UniRef100_D1J858" ] }, "ips": { "uniref100_id": "UniRef100_D1J858", "uniref90_id": "UniRef90_D1J858" }, "psc": { "uniref90_id": "UniRef90_D1J858", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "LMJBGEANGM_637", "locus": "GCA013368015_00624" }, { "type": "cds", "sequence": "contig_1", "start": 359071, "stop": 359358, "strand": "-", "frame": 3, "gene": null, "product": "Large ribosomal subunit protein bL17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "nt": "TTGATTAATGGCAAAGTAACAACCACATTAGAACGTGCTAAAAGAATTCGTTCAAATGCAGAAAAAATGATTACTCTAGGTAAGAAAAATACCCTAGCTTCGCGTAGACAAGCAGCCAAATATTTAAGATTAATTGCAACAGAAAACAAAAATAAAAATTCATTACAATATTTATTTGATGTTGTGGCACCAAAATACGTTGAACGTAATGGTGGATATACAAGAATTACAAAATTAGCCAATCGTGCTGGTGATAATGCTAAAATGGCAATAATCGAATTGGTATAA", "aa": "MINGKVTTTLERAKRIRSNAEKMITLGKKNTLASRRQAAKYLRLIATENKNKNSLQYLFDVVAPKYVERNGGYTRITKLANRAGDNAKMAIIELV", "aa_hexdigest": "624475da950116ad9799080f8a5457a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FA24CA4", "query_cov": 1.063157894736842, "subject_cov": 0.808, "identity": 0.564, "score": 107.0, "evalue": 2.53e-28, "valid": false, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P20277", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20277", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [], "id": "LMJBGEANGM_638", "locus": "GCA013368015_00625" }, { "type": "cds", "sequence": "contig_1", "start": 359505, "stop": 360473, "strand": "-", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P38018", "UniRef:UniRef90_D1J860" ], "nt": "ATGAAGAAAATACAAAAAATTACATATAAAGAATTAGTAGCAGAAAAAGTATCAGATTTTAATACAACATTCGTTATTGAACCACTAATGCGTGGTTATGGTAACACAATAGGAACTGTAATAAGAAGAACATTACTTTCTTCGATTACTTCTGTTGCTCCATTTGCAATAAAAATTAGTCATGTTGAACATGAATTTACTGCAATTGAAGGATTAAGAGAAGATGCTATTACATTAGTTGCAAACATAAGAAAAATTCGCTTTGCATATGACCCAGAATTATTTGAAAAAGATAATTTAGCTAAAATTTCATTTAAATCAACAAAAGAAGGGGAAGTTCATGCTTCGGATATTGAAACTATCCCTGGACTTGACATTGTTAATAAAGATCAATACATAGCAACAATATCAAAAAATGGTTCATTGGAATTTGAATTATATTTAAGAACCGGTAGAGGCTATGTTTCATTTGAAGACAATAAAAAGACCATTCTAGAATATGGTCCAAAATTAGAATCAAAAATCAAAAATGGTCAATTTTTAGCAATGGATAGTGACTTCAGTCCTATTAAAAAAGTTGCGATTTTATTTGAAGAATTAAATTCTACTTCTAACATAATTGAAGAAAGATTAAAAATTAAAGTTGAAACCGATGGAACTGTTGAAGCTAAAAACGTTATGGAAGAAGCAGCAAAGATTATAGTTGCTCATTTCCAAATCATTGGAAACATTGACGCATTAGGAACAATTGATTTATTCGATGATCAAAAAGAAAAACATGAAAAAACTCCAAAAGTTTCAGTTTCTATTGATAAATTGAATTTGACAATTCGTTCATTGAATGCTTTAAGACGTGCTGGATTTAATAATGTTGATGAAATAATGAAATTAAGCGATGAAGAACTTTCAAATATTAAAAATTTGGGTAAAAAATCAGTACAAGATATTATTGACCGTCGTAGAGAATGA", "aa": "MKKIQKITYKELVAEKVSDFNTTFVIEPLMRGYGNTIGTVIRRTLLSSITSVAPFAIKISHVEHEFTAIEGLREDAITLVANIRKIRFAYDPELFEKDNLAKISFKSTKEGEVHASDIETIPGLDIVNKDQYIATISKNGSLEFELYLRTGRGYVSFEDNKKTILEYGPKLESKIKNGQFLAMDSDFSPIKKVAILFEELNSTSNIIEERLKIKVETDGTVEAKNVMEEAAKIIVAHFQIIGNIDALGTIDLFDDQKEKHEKTPKVSVSIDKLNLTIRSLNALRRAGFNNVDEIMKLSDEELSNIKNLGKKSVQDIIDRRRE", "aa_hexdigest": "87e58c6c79dce2574865c9b0167ca5f9", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J860", "query_cov": 1.0, "subject_cov": 0.936046511627907, "identity": 0.997, "score": 608.0, "evalue": 4.63e-219, "valid": true, "gene": "RpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P38018", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P38018", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38018" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [ "rpoA" ], "id": "LMJBGEANGM_639", "locus": "GCA013368015_00626" }, { "type": "cds", "sequence": "contig_1", "start": 360767, "stop": 360877, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGAAAAATAAAAAAGTTATTACTCAAGGTATTGCACATATTCACTCAACTTACCAAAACACAATTGTTTCTTTCTCAGATCTAAAAGGAAATGTTTTTGCTTGA", "aa": "MAKKNKKVITQGIAHIHSTYQNTIVSFSDLKGNVFA", "aa_hexdigest": "a34c7eb380a59aa586242df175c0d8bb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3990.5867000000007, "isoelectric_point": 10.000351905822754 }, "id": "LMJBGEANGM_640", "locus": "GCA013368015_00627" }, { "type": "cds", "sequence": "contig_1", "start": 360880, "stop": 361248, "strand": "-", "frame": 3, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "COG:COG0099", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02952", "RefSeq:WP_012855550.1", "SO:0001217", "UniParc:UPI0001BC2240", "UniRef:UniRef100_D1J862", "UniRef:UniRef50_P38017", "UniRef:UniRef90_D1J862" ], "nt": "ATGGCCAGAGTTTTAAACATTGAAATTCCTAATAATAAAAAAGTTCGTATTTCACTTACTTACATCTTTGGTATTGGCGTTTCACTAGCAAAAGAAATTTTAAAAGATGCTAATGTTGACGGCGAAAAAAGAGTTAAAGATCTTAGCGAAGAAGAATTAACTAGAATCAGAGATGAAGCTAAAAAATACACCACTGAAGGTGATTTAAGAAGAGAAATAAATCTAAACATTAAAAGATTAATGGAAATTAAATCATATCGTGGTATTAGACACCGTAAGGGATTACCTGTACGTGGTCAATCTACAAAGAAAAATGCAAGAACAAGAAAAGGTCCAAGAAAAACAATAGCAGGAAAGAAAGGTAAATAA", "aa": "MARVLNIEIPNNKKVRISLTYIFGIGVSLAKEILKDANVDGEKRVKDLSEEELTRIRDEAKKYTTEGDLRREINLNIKRLMEIKSYRGIRHRKGLPVRGQSTKKNARTRKGPRKTIAGKKGK", "aa_hexdigest": "ac04cc895c8f114982ffe618d49356fa", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2240", "ncbi_nrp_id": "WP_012855550.1", "uniref100_id": "UniRef100_D1J862", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2240", "RefSeq:WP_012855550.1", "UniRef:UniRef100_D1J862" ] }, "ips": { "uniref100_id": "UniRef100_D1J862", "uniref90_id": "UniRef90_D1J862" }, "psc": { "uniref90_id": "UniRef90_D1J862", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "cog_id": "COG0099", "cog_category": "J", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "LMJBGEANGM_641", "locus": "GCA013368015_00628" }, { "type": "cds", "sequence": "contig_1", "start": 361377, "stop": 361592, "strand": "-", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0006413", "KEGG:K02518", "RefSeq:WP_012855552.1", "SO:0001217", "UniParc:UPI0001BC2242", "UniRef:UniRef100_D1J864", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_D1J864" ], "nt": "ATGGCAAAAGACGCAATCAAAATGTCAGGCAAAATTACTAAAATGCACACATCAAGAGACTATGATGTTTTATTAGAAAATGGACTTACTATTAAAGCTTCTATTTCAGGTAAAATGTCACTTCACAATATCAAAATGATCCCTGGGGATTTAGTTGATGTTGAACTTAGTCCATATGATATGAGCAGAGGTAGAATTGTTTTTAGACACAAATAA", "aa": "MAKDAIKMSGKITKMHTSRDYDVLLENGLTIKASISGKMSLHNIKMIPGDLVDVELSPYDMSRGRIVFRHK", "aa_hexdigest": "00b621bb30ccf8e19c9059b63d700fad", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2242", "ncbi_nrp_id": "WP_012855552.1", "uniref100_id": "UniRef100_D1J864", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2242", "RefSeq:WP_012855552.1", "UniRef:UniRef100_D1J864" ] }, "ips": { "uniref100_id": "UniRef100_D1J864", "uniref90_id": "UniRef90_D1J864" }, "psc": { "uniref90_id": "UniRef90_D1J864", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "LMJBGEANGM_642", "locus": "GCA013368015_00629" }, { "type": "cds", "sequence": "contig_1", "start": 361691, "stop": 362344, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0006508", "GO:0070006", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_D1J865" ], "nt": "ATGGTCATAATAAAAAATCAATTTGAAATTGAAAAGATCAAAAAAGCTTGTAAAATCCTGGCAGAAGTGAAAACTGTTTTATACGACTTTATAAGACCAGGCGTTTCTTTAAAAGAAATTGATAGCGTAGCTTTTAAAGAAATAAGAAAAAGGGGTGGAAAACCAGCGTTTTTAGGACAATATGGTTTCCCTAATACATGTTGCATTTCTGTTAATAATGAATTAATTCACGGAATTCCTAGTGATTATGTTGTAAAGGATGGAGATATAGTCAAAATTGACACTGGAGCAATTTATGATGGCTATTATTCTGATTCAGCATTCACAAAAGGAGTTGGCAACATAACTGAAGCAGACAAAAAATTAATTGCCTGCGCAAAAGACGCTTTTTATGCCGGCTTTAATGCAATTAAAGTTGGCAAACACATTGGTGATATTTCTGCAGCCATAGGAAATTTAATTCGTGATAGAGGATATTTTACTCCAGATGAATATACAGGTCACGGAATAGGCAGAAAGTTGCATGAAGATCCATATGTTTATAACGATGGCTATCCAGGAACTGGACAGCTAATTAGAAATGGTATGGTAATTTGCATTGAACCAATGATTTTACAAAAATCTAAAAATGTATTGGTCAAGGGAAATGGATGA", "aa": "MVIIKNQFEIEKIKKACKILAEVKTVLYDFIRPGVSLKEIDSVAFKEIRKRGGKPAFLGQYGFPNTCCISVNNELIHGIPSDYVVKDGDIVKIDTGAIYDGYYSDSAFTKGVGNITEADKKLIACAKDAFYAGFNAIKVGKHIGDISAAIGNLIRDRGYFTPDEYTGHGIGRKLHEDPYVYNDGYPGTGQLIRNGMVICIEPMILQKSKNVLVKGNG", "aa_hexdigest": "f0771c603bdc7f9bf4e85fd5e65ecb54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J865", "query_cov": 1.0, "subject_cov": 0.868, "identity": 1.0, "score": 442.0, "evalue": 1.02e-156, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "cog_id": "COG0024", "cog_category": "J", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "LMJBGEANGM_643", "locus": "GCA013368015_00630" }, { "type": "cds", "sequence": "contig_1", "start": 362335, "stop": 363030, "strand": "-", "frame": 3, "gene": "adk", "product": "adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0005524", "GO:0006139", "GO:0019205", "RefSeq:WP_175936727.1", "SO:0001217", "UniParc:UPI00158B9D88", "UniRef:UniRef100_UPI00158B9D88", "UniRef:UniRef50_Q6KI35", "UniRef:UniRef90_D1J866" ], "nt": "ATGATAGAAAAATCTCAAGAAGTGTACAGCGAACACGCAAAAAAATTTCCTAATATAATTATGCTTGGCGCACCAGGAGCTGGTAAAGGCTCTATTGCAACAAAAATGGTGCAAAATAATGATTATGTTCAGATTTCAACTGGAGATATGTTTAGACAAGAAATTAAAAATCAAACTCCCCTTGGCTTAAAAATAAAATCTATTTTAGATTCAGGAGCATATGTTGATGATTGTATAACAAATCAACTAGTTGAAAAAAAATTAACTGAATTAGTAAGAGAAAATAAACACTTCATATTAGACGGATATCCCCGTACATTAGCTCAAGCAAAGTTTCTACTTTCGTTAGCAAATGTTGGAATCAAAATTGATAAAGTAATTTTACTAGAAATTACTGATAAACAAATTATTGACCGTCTTTCAAAACGTAGAATTTGTTTAAATTGTAAAACCATTTATCATTTGCAATCATTTCCACCATTACCTGGTGATTTGTGTATTAAATGCCACGAAAAAGTAACAAAAAGACCAGATGACGAACCAGAAGTAATAAAGAAACGTTTATCAATATATAATGAACAAACTAAATGTTTGATCAATTTTTATCAAGAACTTGGTATACTTTTAAAGATAAATAGTTATCAAACAATTGATAAAGTATATAATGATGTAGAAGAGGCTTTAAAATGGTCATAA", "aa": "MIEKSQEVYSEHAKKFPNIIMLGAPGAGKGSIATKMVQNNDYVQISTGDMFRQEIKNQTPLGLKIKSILDSGAYVDDCITNQLVEKKLTELVRENKHFILDGYPRTLAQAKFLLSLANVGIKIDKVILLEITDKQIIDRLSKRRICLNCKTIYHLQSFPPLPGDLCIKCHEKVTKRPDDEPEVIKKRLSIYNEQTKCLINFYQELGILLKINSYQTIDKVYNDVEEALKWS", "aa_hexdigest": "21ac583ebb1324936e7ef4da77fb1c63", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00158B9D88", "ncbi_nrp_id": "WP_175936727.1", "uniref100_id": "UniRef100_UPI00158B9D88", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B9D88", "RefSeq:WP_175936727.1", "UniRef:UniRef100_UPI00158B9D88" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B9D88", "uniref90_id": "UniRef90_D1J866" }, "psc": { "uniref90_id": "UniRef90_D1J866", "gene": "Adk", "product": "adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q6KI35", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005524", "GO:0006139", "GO:0019205" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KI35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KI35" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "LMJBGEANGM_644", "locus": "GCA013368015_00631" }, { "type": "cds", "sequence": "contig_1", "start": 363027, "stop": 363458, "strand": "-", "frame": 1, "gene": "secY", "product": "SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78", "UniRef:UniRef90_UPI0021BD3D78" ], "nt": "TTGCAATTTACTCAACCACTTGGATTCTTTCTATTTATTTTAATTACATTTGGATTAACAATATTAATGGGAATCCAACAATCAAGAATTGATAAAATTAGTGAAGATTTTGCTAAAAATAGTACTTTTATTCCTGGCGTTAGACCCGGAGAACAAACAGAAGATTATTTATTGAATGTAATTTTACGTTTATCATTCTTTTCTTCAGGATATTTATTGTTGCTAGGCGCTTTACAATTTATTCAACAAATGCTAGGCATGCCAGCCTCAATAGCATTTGGCGGTACAAGTATAATGATACTAGTTTCAACGGCTTATGAAACTGTTCAACAAATTAAAGCCCGCTACAAGTCACAAGAATTATCAAGAAAGAGAAAAATGATAAGAGAATTAAAAGAAATGTATGGAGAGGAAGAAGAAGATTTAATATGA", "aa": "MQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLI", "aa_hexdigest": "85abb75e04e5391b92af8f76c1e9a417", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secY" ], "truncated": "3-prime", "pseudogene": { "start": 363027, "stop": 363758, "observations": { "mutation": [ 363473 ], "stop": [ 363473 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021BD3D78", "query_cov": 1.0, "subject_cov": 0.5296296296296297, "identity": 0.867, "score": 243.0, "evalue": 5.13e-79, "gene_start": 1, "gene_end": 143, "reference_start": 127, "reference_end": 269, "reference_sequence": "MLWFFVGIASGLIPSFKSAFQYYIPSAKQTNLVLQEIINYAVYILVYLLTIFIVVIFTIAERRIPIQQVGAGLSKNAEELSYLPLKANPAGIMSVIFSMMILGVPTMIANMLNPNTSRYYQWVYANLQFTQPLGFFLFILITFGLTILMGIQQSKIDKISEDFAKSSTYIPGIRPGEQTETYLLNVVLRLSFFSSGFLIVLGSLQFVQQMIGMPASIAFGGTTVMILVSTAYETIQQIKARYKSQELARKRRTIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.8559670781893004, "score": 414.0, "evalue": 5.4e-149, "description": "Internal stop codon at 363473. Nonsense mutation around 363473.", "psc": { "uniref90_id": "UniRef90_UPI0021BD3D78", "product": "SecY", "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021BD3D78", "UniRef:UniRef50_UPI0021BD3D78" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78" ] } }, "id": "LMJBGEANGM_645", "locus": "GCA013368015_00632" }, { "type": "cds", "sequence": "contig_1", "start": 363471, "stop": 364244, "strand": "-", "frame": 1, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_A0A448ZXS5", "UniRef:UniRef90_D1J867" ], "nt": "TTGAATTTAGTAGGTGGCGGTGGATTAAGACAATTCTCTGTTGTCGCGCTAGGAATAGGGCCGTTTATATCATCTAGTTTAGTTATGATGATATTGCAAACTAAAGCATTCCCGGCCATTCACCGTTTAAGTCAATCTGGTCCTCAAGGCCGGATTAAAATAAATTTCATTACTTACGGAGTTACTTTTGTTTTTGCAATAGTTCAAGCATATTTGATAACAAAAGCATTAGTTAATCCTCGTCAAGGATTTGGAATTACTTTTTCACCAAAATTAGAAAAATTATTTGGACAAAGTGGAATGGTAGCATATCAATATTTCATTTTGCCTGCAATATTAGTAGCTGGCTCATTCTTTTCATTGTTCTTATCAGAACAAATAACTAATAGAGGCGTTGGCAATGGAACGAGTTTAATAATCTTTGTTGGTATAGCAAGTGCTTTAATTCCTTCATTCAAGAGTGCATTTAAATTTTATGTTCCATCAGCTCAACAAAATAATTTAGTTTTACAAGAAGCAATAAATTACGCAGTATATATCTTTTGCTATTTACTTGTAATATTTATTGTTGTAATATTTACAATTGCAGAAAGACATATTCCAATTCAACAAGTTGGTGCGGGTCTTTCTAAAAACGAAAAAGAATTGAGTTATTTACCTATTAAAGCAAATCCTGCTGGTATCATGTCAGTTATCTTCTCAATGATGATATTGAGTGTGCCAACAATGATTGCAAACTTATTTGATCCAAATACAAGCAAGTATTATCAATGA", "aa": "MNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQ", "aa_hexdigest": "36e165d8b8faf321a6d6ce6ece0a03ce", "start_type": "TTG", "rbs_motif": "AGGAG(G)/GGAGG", "genes": [ "secY" ], "truncated": "both", "pseudogene": { "start": 363168, "stop": 364493, "observations": { "mutation": [ 363473, 364385, 364388, 364397 ], "stop": [ 363473, 364385, 364388, 364397 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J867", "query_cov": 1.0, "subject_cov": 0.5255623721881391, "identity": 0.996, "score": 489.0, "evalue": 4.52e-171, "gene_start": 1, "gene_end": 257, "reference_start": 84, "reference_end": 340, "reference_sequence": "MARKTKLEKEISQEKIERKLALDKFLVDKKNTWNEWWKNHNLFKKILFTLFIITLFLATGTITIPGVNLANPDSLTQGDFFGILNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQWVYNNLQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.9909297052154195, "score": 839.0, "evalue": 2.53e-311, "description": "Internal stop codon at 363473, 364385, 364388, 364397. Nonsense mutation around 363473, 364385, 364388, 364397.", "psc": { "uniref90_id": "UniRef90_D1J867", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A448ZXS5", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0015031", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J867", "UniRef:UniRef50_A0A448ZXS5", "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXS5" ], "product": "Protein translocase subunit SecY" } }, "id": "LMJBGEANGM_646", "locus": "GCA013368015_00633" }, { "type": "cds", "sequence": "contig_1", "start": 364395, "stop": 364493, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACTAAGTTGGAAAAAGAGATTAGTCAAGAAAAGATCGAGCGCAAATTAGCACTAGATAAGTTTTTAGTTGATAAAAAAAACACTTGA", "aa": "MARKTKLEKEISQEKIERKLALDKFLVDKKNT", "aa_hexdigest": "52b053d98925f993c9317f2b09f4dc46", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3832.5150000000012, "isoelectric_point": 9.775808525085449 }, "id": "LMJBGEANGM_647", "locus": "GCA013368015_00634" }, { "type": "cds", "sequence": "contig_1", "start": 364493, "stop": 364927, "strand": "-", "frame": 2, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855556.1", "SO:0001217", "UniParc:UPI0001BC2246", "UniRef:UniRef100_D1J868", "UniRef:UniRef50_Q601J6", "UniRef:UniRef90_D1J868" ], "nt": "ATGCAATTACATAATTTAAAACCAACTCCAGGGGCAAGAAAAGACAAACACCGTGTTGGTCGTGGTCATGCTGCTGGAAAAGGAAAACAAGCCGGTAGAGGACAAAGTGGTCAAACAAAAAGAAGCAAAGTAAGACTAGGATTTGAAGGTGGCCAATTGCCACTATTTAGAAGAATTCCAAAACGTGGATTTAGCAATGTAAATCACGTTGAATTCCAAGTAGTAAATCTAGCAGATTTACAATCAAAATATCAAGATGGTGAAGTAGTTTCTTACGAAACACTTGCTGCAAGAAACTTGATTAAAGGAACATTACCAGTTAAAATTTTAGCTAAGGGTTCTTTAACTAAAAAACTTAATGTACAAATTCCAACACTTTCACAATCCGCTATTGAAGCTATTGAAAAAGTCGGAGGAAAAATAGAGGTTAAATAA", "aa": "MQLHNLKPTPGARKDKHRVGRGHAAGKGKQAGRGQSGQTKRSKVRLGFEGGQLPLFRRIPKRGFSNVNHVEFQVVNLADLQSKYQDGEVVSYETLAARNLIKGTLPVKILAKGSLTKKLNVQIPTLSQSAIEAIEKVGGKIEVK", "aa_hexdigest": "e48e82061d297ab366d048e8a4e1e848", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2246", "ncbi_nrp_id": "WP_012855556.1", "uniref100_id": "UniRef100_D1J868", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2246", "RefSeq:WP_012855556.1", "UniRef:UniRef100_D1J868" ] }, "ips": { "uniref100_id": "UniRef100_D1J868", "uniref90_id": "UniRef90_D1J868" }, "psc": { "uniref90_id": "UniRef90_D1J868", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_Q601J6", "cog_id": "COG0200", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q601J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q601J6" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "LMJBGEANGM_648", "locus": "GCA013368015_00635" }, { "type": "cds", "sequence": "contig_1", "start": 364937, "stop": 365605, "strand": "-", "frame": 2, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02988", "RefSeq:WP_020002417.1", "SO:0001217", "UniParc:UPI000367CA6D", "UniRef:UniRef100_A0A454CA16", "UniRef:UniRef50_L0RWI9", "UniRef:UniRef90_D1J869" ], "nt": "ATGGCTGAAGTAGAAAAAAATATTAAAAATTCAGAAAAACCTGAATTAAAAGAAAATAAAACAGAAGGCACAAAGGTTGTTTCTTTTAAAAGAGAAAGATCAAATTCAAAAGATCTAAAATCAAGCGAAAATAAACCAAAACAATTCCAAAATCGTAATCAAGATCATTCAGCAAATCAAGCTCAAGCAAAACAATTCGAAGAAAAAGTTATTGACATTGCAAGAGTTACAACTGTTGTTAAGGGTGGACGTAGATTTTCATTCTCAGCTTATGTTGTTGTTGGTGACAAAAAAGGAAAAGTTGGATTTGGACATGGAAAAGCTAATGAAGTCCAAGATGCAATTAAAAAAGCAGTTAAAGATGCACAAAAAAATGTTTTCTCAGTTCCAATTTCAAATGGAACAATCCCTCATGAAATTCAAGATAAATTCTTAGCATCAAGAATCCAATTACGTCCAGCTCCAAAAGGTGCCGGAATCATTGCTTCAAACACCGTTCGTGCTGTTGTTGAATTAGCAGGTTACACCGACATTTCAACAAAAACTTATGGTTCGAGAACAAAACAAAACATTGTCCAAGCTACTATTAAGGCACTTAAAAAAGTTAGAACTGTTGAAGAAATCGCAAAATTACGCGATATTGATGTAAATCATCTATTATCAAAATAA", "aa": "MAEVEKNIKNSEKPELKENKTEGTKVVSFKRERSNSKDLKSSENKPKQFQNRNQDHSANQAQAKQFEEKVIDIARVTTVVKGGRRFSFSAYVVVGDKKGKVGFGHGKANEVQDAIKKAVKDAQKNVFSVPISNGTIPHEIQDKFLASRIQLRPAPKGAGIIASNTVRAVVELAGYTDISTKTYGSRTKQNIVQATIKALKKVRTVEEIAKLRDIDVNHLLSK", "aa_hexdigest": "bcbc4611471dda464e1e9604f871d769", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI000367CA6D", "ncbi_nrp_id": "WP_020002417.1", "uniref100_id": "UniRef100_A0A454CA16", "db_xrefs": [ "SO:0001217", "UniParc:UPI000367CA6D", "RefSeq:WP_020002417.1", "UniRef:UniRef100_A0A454CA16" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA16", "uniref90_id": "UniRef90_D1J869" }, "psc": { "uniref90_id": "UniRef90_D1J869", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_L0RWI9", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWI9" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "LMJBGEANGM_649", "locus": "GCA013368015_00636" }, { "type": "cds", "sequence": "contig_1", "start": 365605, "stop": 365952, "strand": "-", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855558.1", "SO:0001217", "UniParc:UPI0001BC2248", "UniRef:UniRef100_D1J870", "UniRef:UniRef50_A0A6M4JHS6", "UniRef:UniRef90_D1J870" ], "nt": "ATGTTATCAAGAAATTTAAAACGTAAAGCAAAACACTTAAAGATTACTAACAAATTGTCAAAAGGAACTGCATTAGTTCCTAGAGTTGTTGTTTTTAAATCTTTACATGCATTTTATGCACAAGCAGTAAATGATGAATTGCACACTACAATTGCTTCTTCATCAACACAAATACTAGAAAATAAAGCAAACAATATTGATGCAGTTAAATTAGTTGCAAAAGATTTTGCTAAAAAATTATTGGCAATCAATGTAAAATCAATAGTGTTTGATCGTTCAGGATACATATACCACGGAAAATTAGCAGCTTTTGCTGATACTCTAAGAGAAGAAGGGATTAAATTCTAA", "aa": "MLSRNLKRKAKHLKITNKLSKGTALVPRVVVFKSLHAFYAQAVNDELHTTIASSSTQILENKANNIDAVKLVAKDFAKKLLAINVKSIVFDRSGYIYHGKLAAFADTLREEGIKF", "aa_hexdigest": "4512354e3b284c6d5db3f37ab085dddf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2248", "ncbi_nrp_id": "WP_012855558.1", "uniref100_id": "UniRef100_D1J870", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2248", "RefSeq:WP_012855558.1", "UniRef:UniRef100_D1J870" ] }, "ips": { "uniref100_id": "UniRef100_D1J870", "uniref90_id": "UniRef90_D1J870" }, "psc": { "uniref90_id": "UniRef90_D1J870", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_A0A6M4JHS6", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JHS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JHS6" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "LMJBGEANGM_650", "locus": "GCA013368015_00637" }, { "type": "cds", "sequence": "contig_1", "start": 365974, "stop": 366513, "strand": "-", "frame": 3, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_012855559.1", "SO:0001217", "UniParc:UPI0001BC2249", "UniRef:UniRef100_D1J871", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_D1J871" ], "nt": "ATGTCTAGAATTGGAAAAAGAGTTTTAGAAATACCAAAAGATGTTGAAGTTAAATTAGATAAAAACCATATCGAAGTAAAAGGCAAATTAGGAGAATTGAGTTATACATTCTCACCATTAATTTCAGTAGTTGTTGAAAACAACGAAATTAAGACATTGAGATCTAACGAAGAAAAATCTACAAAACAATTACACGGAACAACAAATGCAAACATTAAAAATATGTTAATAGGTGTTTCAAGTGGTTACAAAAAAGAAATTGAAATTAAAGGTGTAGGTTACAAAGCCACCTTAAAGGGAAATACAATTGAAATTATTGCTGGTTATTCACATGCTGTAAATTTAGAAGTTCCATCAAATCTAAAAATTGAATTACCAAAACCAACCAATATTATTATTTCAGGAATTGACAAACAAGCTGTTGGTGAATTTGCTGCAAATATTCGTGATGTAAGACGTCCAAGTCCATATTCTGGAAAAGGTATTATGTATAAAGATGAAGTTATTAGACGTAAGGAAGGAAAGACAGCTGCTAAATAG", "aa": "MSRIGKRVLEIPKDVEVKLDKNHIEVKGKLGELSYTFSPLISVVVENNEIKTLRSNEEKSTKQLHGTTNANIKNMLIGVSSGYKKEIEIKGVGYKATLKGNTIEIIAGYSHAVNLEVPSNLKIELPKPTNIIISGIDKQAVGEFAANIRDVRRPSPYSGKGIMYKDEVIRRKEGKTAAK", "aa_hexdigest": "e98ddf5827d0489bd840bf7f811c766a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2249", "ncbi_nrp_id": "WP_012855559.1", "uniref100_id": "UniRef100_D1J871", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2249", "RefSeq:WP_012855559.1", "UniRef:UniRef100_D1J871" ] }, "ips": { "uniref100_id": "UniRef100_D1J871", "uniref90_id": "UniRef90_D1J871" }, "psc": { "uniref90_id": "UniRef90_D1J871", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "LMJBGEANGM_651", "locus": "GCA013368015_00638" }, { "type": "cds", "sequence": "contig_1", "start": 366538, "stop": 366948, "strand": "-", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_D1J872" ], "nt": "ATGGCTATTATTATAGATCCAGTTGCAGATATGTTTGTACGTATTAAAAATGCAATTTCTCGTAAATATCATGAAGTTGTATTGCCACATTCAAACAAGAAAGCAAAAATTTTAGAAATTATTAAAAGAGAAGGATATATTGTTGATTTTGAAGTTGTAAAAAACGAAAAAACCAATTTTAAAGAAATAAAAGTTACTTTAAAATACAAAGGTATGAATCAAAATCAATCAGCAATTTCTGGTCTTAAAAAAGTTTCAAAACCTGGCTTAAAAGTATATTCATCTGCTGAAAAATTGCCTAGAGTTTTAAGTGGATATGGAACAGCTATTATTTCAACATCAAAAGGTTTATTAACAGATAAAGAAGCTCGTAAGGCTAATGTTGGTGGCGAAGTTATCGCCTTCATTTGA", "aa": "MAIIIDPVADMFVRIKNAISRKYHEVVLPHSNKKAKILEIIKREGYIVDFEVVKNEKTNFKEIKVTLKYKGMNQNQSAISGLKKVSKPGLKVYSSAEKLPRVLSGYGTAIISTSKGLLTDKEARKANVGGEVIAFI", "aa_hexdigest": "44621eacfa9ce74a148bdad8879e46d6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J872", "query_cov": 1.0, "subject_cov": 0.9927007299270073, "identity": 1.0, "score": 256.0, "evalue": 4.4e-86, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "LMJBGEANGM_652", "locus": "GCA013368015_00639" }, { "type": "cds", "sequence": "contig_1", "start": 366954, "stop": 367136, "strand": "-", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "nt": "ATGGCAAGAACATCATTAAAGGTTAAAGCCGAACGCCAACCTAAATTTAAGGTTAGAAAATATACTCGTTGCCAACTTTGTGGCCGTGTTCACGCTGTATTGCGTAAATATAAAATTTGTAGAATTTGTTTTAGAAAATTAGCACATGAAGGAAAACTTCCTGGTGTTAAGAAAGCGAGCTGA", "aa": "MARTSLKVKAERQPKFKVRKYTRCQLCGRVHAVLRKYKICRICFRKLAHEGKLPGVKKAS", "aa_hexdigest": "4b14d9dfdd9d224ff7a908e546cd8f55", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A448ZVU2", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.85, "score": 106.0, "evalue": 2.85e-29, "valid": false, "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_Q9PQP7", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [], "id": "LMJBGEANGM_653", "locus": "GCA013368015_00640" }, { "type": "cds", "sequence": "contig_1", "start": 367463, "stop": 367708, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGGCAAAACAAATTAAATTAGCATTAGAACAAAAATATTTAGACGAAATTCGTTTCGCGCTAGTTAAAGAATTTGGTTACACCAGTGTAATGCAAGCTCCTAAACTAGAAAAAATCGTTATTAACATGACTGCCGGTAACGAAGTTTCAAACTCAAAGGCAATCGAAGAAGTTATGGTTGAACTTGCTCAAATTACAGGTCAAAAACCATATCAAACAACTGCTAAGAAATCATTGGCTTCATGA", "aa": "MAKQIKLALEQKYLDEIRFALVKEFGYTSVMQAPKLEKIVINMTAGNEVSNSKAIEEVMVELAQITGQKPYQTTAKKSLAS", "aa_hexdigest": "7a5beec12a4a6cd8a099cdf0e8effdc6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.8888888888888888, "subject_cov": 0.8571428571428571, "identity": 0.569, "score": 79.7, "evalue": 5.85e-18, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "LMJBGEANGM_654", "locus": "GCA013368015_00641" }, { "type": "cds", "sequence": "contig_1", "start": 367786, "stop": 368058, "strand": "-", "frame": 3, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_A0A448ZW26", "UniRef:UniRef90_A0A454CAH0" ], "nt": "ATGGCTCAAGCTAAAATTAGAAAAAATGACATGGTATTAATTATTGCTGGTAAAGACAAAGGAAAATTTGCTGCTGTTTTATCAACAAATCAAAAAGAACAAACTGTTGTTTTAAAAGGCGTAAACATTAAAACTAAACACCATAAACCATCACAAGAAAATAATGATGGAAAAATTGAAAAGAAAGAATACCCAATTCATATTTCAAACGTTGCTTATTTGGTTAAAAAAGGTGCTCAAGGTCAAAAATCAATCGGTTCAAAAATTGGTTGA", "aa": "MAQAKIRKNDMVLIIAGKDKGKFAAVLSTNQKEQTVVLKGVNIKTKHHKPSQENNDGKIEKKEYPIHISNVAYLVKKGAQGQKSIGSKIG", "aa_hexdigest": "751bca10ea367ed894847567bc73e234", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A454CAH0", "query_cov": 1.0, "subject_cov": 0.8108108108108109, "identity": 1.0, "score": 173.0, "evalue": 1.23e-54, "valid": true, "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_A0A448ZW26", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW26" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "LMJBGEANGM_655", "locus": "GCA013368015_00642" }, { "type": "cds", "sequence": "contig_1", "start": 368060, "stop": 368428, "strand": "-", "frame": 2, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_012855564.1", "SO:0001217", "UniParc:UPI0001BC229F", "UniRef:UniRef100_D1J876", "UniRef:UniRef50_A5FMZ0", "UniRef:UniRef90_D1J876" ], "nt": "ATGCTTAGTGAATTTTCAAGATGTAACGTTGCTGACAATTCAGGCGCAAAAGAAGTTATGATTATTAAAAACCTAGGTGGATCAATTGTTAGATCAACCAATATTGGTGATATTGTTGTTGTAACTATTAAAAAAGCTATTCCAAATGGAATTGTAAAAGAAGGCCAAGTTGTTAAAGCGGTTATAGTTAGAACAAAACGTGGTTTAATGCGTGAAAATGGTTCTCATATAAGATTTGATGACAATGCTGTTGTATTAATTAAGGAAGATAACTCATTAAGAGGAACTCGGGTATTTGGTCCAGTAGCAAGAGAATTACGTGAAAAAGGTTTTTTAAAGATTGTTTCATTAGCTCCGGAGGTTTTATAA", "aa": "MLSEFSRCNVADNSGAKEVMIIKNLGGSIVRSTNIGDIVVVTIKKAIPNGIVKEGQVVKAVIVRTKRGLMRENGSHIRFDDNAVVLIKEDNSLRGTRVFGPVARELREKGFLKIVSLAPEVL", "aa_hexdigest": "c87871ee0223ca5673069e09b0bae576", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC229F", "ncbi_nrp_id": "WP_012855564.1", "uniref100_id": "UniRef100_D1J876", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC229F", "RefSeq:WP_012855564.1", "UniRef:UniRef100_D1J876" ] }, "ips": { "uniref100_id": "UniRef100_D1J876", "uniref90_id": "UniRef90_D1J876" }, "psc": { "uniref90_id": "UniRef90_D1J876", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A5FMZ0", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A5FMZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5FMZ0" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "LMJBGEANGM_656", "locus": "GCA013368015_00643" }, { "type": "cds", "sequence": "contig_1", "start": 368421, "stop": 368705, "strand": "-", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855565.1", "SO:0001217", "UniParc:UPI0001BC22A0", "UniRef:UniRef100_D1J877", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_D1J877" ], "nt": "ATGGAAACTACAGTTAGAGAAAACCGTCGTAAAACTTTAGTTGGAACAGTTGTATCAACTAAAAACCAAAAAACAATTACTGTTGTTGTAGAAATTTACGAAAAACACCCTCTTTATTCAAAACGTTTTAAAAAATCAAAGAAATTTGCAGTTCATGATGAACAAAATTTAGCTAAAGTTGGTGACATTGTTAAAATTCAAGAAACAAGACCTTTATCAAAAACCAAACACTTTAGACTAGTAGAAATCAAATCACACGCAATTGAAGGAGCAGAAAATGCTTAG", "aa": "METTVRENRRKTLVGTVVSTKNQKTITVVVEIYEKHPLYSKRFKKSKKFAVHDEQNLAKVGDIVKIQETRPLSKTKHFRLVEIKSHAIEGAENA", "aa_hexdigest": "ea20c26ef5b5db251866ea7f325bed04", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001BC22A0", "ncbi_nrp_id": "WP_012855565.1", "uniref100_id": "UniRef100_D1J877", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A0", "RefSeq:WP_012855565.1", "UniRef:UniRef100_D1J877" ] }, "ips": { "uniref100_id": "UniRef100_D1J877", "uniref90_id": "UniRef90_D1J877" }, "psc": { "uniref90_id": "UniRef90_D1J877", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "LMJBGEANGM_657", "locus": "GCA013368015_00644" }, { "type": "cds", "sequence": "contig_1", "start": 368705, "stop": 368905, "strand": "-", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855566.1", "SO:0001217", "UniParc:UPI0001BC22A1", "UniRef:UniRef100_D1J878", "UniRef:UniRef50_A0A3B0PQ96", "UniRef:UniRef90_D1J878" ], "nt": "ATGACATTTGCAGAATTAAAAGAAAAATCACTTGACGAACTAAATAAGTTGGTTGCTGATTTAAGAGCAGAACTATTCTCATTACGTTTCAAAAATGCTACAAGACAATTAGACGAAACCCACAAAATCCAATTAGTTAAAAAAGATATCGCAAGAACTTTAACAGCAATTAAAGCAAAAACTTTAGAAGGGAATAAATAA", "aa": "MTFAELKEKSLDELNKLVADLRAELFSLRFKNATRQLDETHKIQLVKKDIARTLTAIKAKTLEGNK", "aa_hexdigest": "cb17ed1367064946a7cec20043621673", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A1", "ncbi_nrp_id": "WP_012855566.1", "uniref100_id": "UniRef100_D1J878", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A1", "RefSeq:WP_012855566.1", "UniRef:UniRef100_D1J878" ] }, "ips": { "uniref100_id": "UniRef100_D1J878", "uniref90_id": "UniRef90_D1J878" }, "psc": { "uniref90_id": "UniRef90_D1J878", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A0A3B0PQ96", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ96" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "LMJBGEANGM_658", "locus": "GCA013368015_00645" }, { "type": "cds", "sequence": "contig_1", "start": 369079, "stop": 369186, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGACGTGAAGGAAAAGTTATTATTAAAATATTCCCTCACATGTCAAAAACTAGTAAACCTATTGGTGTTCGTATGGGATCTGGTAAGGGTAGCCCAGATCAATGA", "aa": "MGREGKVIIKIFPHMSKTSKPIGVRMGSGKGSPDQ", "aa_hexdigest": "06b42c6d46d5e2a7d1d7dd09ca724493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.938154007762737e-10, "score": 35.33918762207031, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3755.4388000000017, "isoelectric_point": 10.564772605895993 }, "id": "LMJBGEANGM_659", "locus": "GCA013368015_00646" }, { "type": "cds", "sequence": "contig_1", "start": 369235, "stop": 369360, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAACCAAAAAGAACTAAACATAGAAAAATGTTCCGTATCCGCCACGACAAAGTTGTTGCACACCGTAATAATACAGTTGCATTTGGCGAATTTGGTTTAAAATCAACTTCATCAGCATGA", "aa": "MLQPKRTKHRKMFRIRHDKVVAHRNNTVAFGEFGLKSTSSA", "aa_hexdigest": "7c21f6da5b3d095a3e3e708e02acb1c9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4781.533299999998, "isoelectric_point": 11.849047279357908 }, "id": "LMJBGEANGM_660", "locus": "GCA013368015_00647" }, { "type": "cds", "sequence": "contig_1", "start": 369455, "stop": 369715, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "TTGCTAGCAGAAGGAATTGCAATTAAATTAGAAAATCGTGGTAGTTTTAGACTAGCACAAAAATTTGCAATTAAAGCAGCATTAAAAGCTGGCGCAAAAGGTATTAAAACTTCAGTAGCTGGACGTCTAAATGGCGTTGATATGGCTAGAACAGAAGGATATACCGAAGGTGAAATGAAATTACATACTTTAAGACAAGATGTTGATTATGCTGTAACAACAGCCTTAACAACATATGGTATCTTAGGAGTTAAGGTATGA", "aa": "MLAEGIAIKLENRGSFRLAQKFAIKAALKAGAKGIKTSVAGRLNGVDMARTEGYTEGEMKLHTLRQDVDYAVTTALTTYGILGVKV", "aa_hexdigest": "12fab75bd1d3b9296d32e0538593a4dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 81, "aa_cov": 0.9418604651162791, "hmm_cov": 1.0, "evalue": 2.4223426622158854e-32, "score": 106.8982925415039, "start": 6, "stop": 86 } ], "seq_stats": { "molecular_weight": 9199.638499999995, "isoelectric_point": 9.752470970153805 }, "id": "LMJBGEANGM_661", "locus": "GCA013368015_00648" }, { "type": "cds", "sequence": "contig_1", "start": 370069, "stop": 370458, "strand": "-", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_012855569.1", "SO:0001217", "UniParc:UPI0001BC22A4", "UniRef:UniRef100_Q8GM55", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_Q8GM55" ], "nt": "ATGGCTAAAGAAATTTTACAAAATTCAGCACATGCATCAGTTCGCATGCAAAGAATTTCACCACGTAAAGCAAGATTAGTAGCAGATTTAATTAGATATAAATCAGCAACTCAAGCAATAGTTATTCTAAAACATACACACAAAAAAGCGTCAGAAATTATTCTAAAACTTCTAAATTCAGCAATTGCTAATGCAACAAATAATGCTGGACTTGATGCAACTAAACTTTATGTAACTACCATTTTAGTAAATGATGGACCTACACTAAAAAGATTCCAACCTCACTCACGTGGACGTGCTTATGCAATTTTAAAAAGAACAAGTCATTTCTTTATTGAATTAACAGAAATCAATAATGTTGAAGAAATCAACAAAAAAGGAGATAAATAA", "aa": "MAKEILQNSAHASVRMQRISPRKARLVADLIRYKSATQAIVILKHTHKKASEIILKLLNSAIANATNNAGLDATKLYVTTILVNDGPTLKRFQPHSRGRAYAILKRTSHFFIELTEINNVEEINKKGDK", "aa_hexdigest": "7a8b68311e501c927669bb50a65848bd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC22A4", "ncbi_nrp_id": "WP_012855569.1", "uniref100_id": "UniRef100_Q8GM55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A4", "RefSeq:WP_012855569.1", "UniRef:UniRef100_Q8GM55" ] }, "ips": { "uniref100_id": "UniRef100_Q8GM55", "uniref90_id": "UniRef90_Q8GM55" }, "psc": { "uniref90_id": "UniRef90_Q8GM55", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "LMJBGEANGM_662", "locus": "GCA013368015_00649" }, { "type": "cds", "sequence": "contig_1", "start": 370633, "stop": 370737, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTTCATTAAAGAAAGCTCCCTTTGTTGATGATCATTTAATGAAGAAAGTTTTGGCTATTATTGAAAATAAATCACCAAAACGTCCAATTAAAACATGA", "aa": "MARSLKKAPFVDDHLMKKVLAIIENKSPKRPIKT", "aa_hexdigest": "5da938756da5c11a053df77e46912a7d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3904.734700000001, "isoelectric_point": 10.383809852600098 }, "id": "LMJBGEANGM_663", "locus": "GCA013368015_00650" }, { "type": "cds", "sequence": "contig_1", "start": 370824, "stop": 371582, "strand": "-", "frame": 1, "gene": null, "product": "Large ribosomal subunit protein uL2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "nt": "ATGGCTATTAAAAAATTTAAAGCATATACCAATGGCCGTCGTAATATGTCTTCTCTTGATTATCAAGCAAATTTAACAGGACATGCTCCTGAAAAATCATTGCTTGTTGCTCTTCCAACAAAAGCAGGAAGAAACAATCAAGGTAAAATCACTACCCGTCATCAAGGTGGTAGATTGAAAAGATTCTATCGTATCATTGATTTTAAACGTCAAAAAGATGGAATTCCAGCTACTGTAAAAACTATTGAATATGACCCTAATCGTTCAGCTAATATTTCATTAGTTGTTTATAGAGATGGTGAAAAAAGATATATTTTAAGCCCTAAAGGTATTAAAATTGGACAAGTTATTTTGTCAGGTGATAATGTTGACATTCAAGTCGGTAACCATTTACCATTAGCCAACATTCCTGAAGGTACATTTGTTCACAATATTGAATTACAACCAAGACAAGGTGGAATCATTGCTAGATCAGCTGGATCATCAGCACAAATCTTAGGTAAAGATGAATCAGGTCGTTATGTAATTCTAAGATTAAAATCAGGCGAAGTTAGAAAAGTTTTAGCAATTAATCGTGCAACAGTTGGTGAAGTAGGAAACGAAGAACACTCACTTGTTAATATTGGTAAGGCAGGACGTAACCGTTTAAGAGGAATTAGACCAACAGTTCGTGGTTCAGCTATGAACCCTAATGATCACCCACATGGTGGTGGTGAAGGTAGACAACCTATTGGTAGAAAGAGTCCTATGACTCCATGA", "aa": "MAIKKFKAYTNGRRNMSSLDYQANLTGHAPEKSLLVALPTKAGRNNQGKITTRHQGGRLKRFYRIIDFKRQKDGIPATVKTIEYDPNRSANISLVVYRDGEKRYILSPKGIKIGQVILSGDNVDIQVGNHLPLANIPEGTFVHNIELQPRQGGIIARSAGSSAQILGKDESGRYVILRLKSGEVRKVLAINRATVGEVGNEEHSLVNIGKAGRNRLRGIRPTVRGSAMNPNDHPHGGGEGRQPIGRKSPMTP", "aa_hexdigest": "2d1053e9b4b069d6e507646decf318a5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3M0A1P8", "query_cov": 1.0, "subject_cov": 0.896797153024911, "identity": 0.897, "score": 457.0, "evalue": 1.04e-161, "valid": false, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [], "id": "LMJBGEANGM_664", "locus": "GCA013368015_00651" }, { "type": "cds", "sequence": "contig_1", "start": 371678, "stop": 372109, "strand": "-", "frame": 2, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855572.1", "SO:0001217", "UniParc:UPI00000BF6FA", "UniRef:UniRef100_D1J884", "UniRef:UniRef50_Q98PY3", "UniRef:UniRef90_D1J884" ], "nt": "ATGAATATAAATGAAGTTATTAAATACCCACTTTTAACAGAAAAAAGTGAATTATCACGTGAAAATAAAAACGTTTATACATTTGTAGTTGATAAAAGAACAAACAAAATCGAAGTTAAAAAAGCAGTTGAATTTATTTTTGATGTAAAAGTATTAAAAGTTAACATTACTAATTACGATAAAAAACCTGCTAAATTAGGACGTTTCTCAGGTTTTAAAAATTCAGTTAAAAAAGCAACTGTATATTTAGCAAAAGATTCAAAAATTATTCTATTTGCTGAAGAATTAAAGAAAGCTAACAAAGAAGCTGAAAAAGCAACAGCAGAAAAAGAAGTTAAAGAAGAAAAACCTGCTGAACCTACCGCTGCAGAATTAAAAGCAGCAGAAAAAATTAAAGCAGCTGAAGCTAAAAAAGCTGCTGAAAACAAATAG", "aa": "MNINEVIKYPLLTEKSELSRENKNVYTFVVDKRTNKIEVKKAVEFIFDVKVLKVNITNYDKKPAKLGRFSGFKNSVKKATVYLAKDSKIILFAEELKKANKEAEKATAEKEVKEEKPAEPTAAELKAAEKIKAAEAKKAAENK", "aa_hexdigest": "e9516e60a03d444412cf50f35f7ebe06", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00000BF6FA", "ncbi_nrp_id": "WP_012855572.1", "uniref100_id": "UniRef100_D1J884", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BF6FA", "RefSeq:WP_012855572.1", "UniRef:UniRef100_D1J884" ] }, "ips": { "uniref100_id": "UniRef100_D1J884", "uniref90_id": "UniRef90_D1J884" }, "psc": { "uniref90_id": "UniRef90_D1J884", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_Q98PY3", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY3" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "LMJBGEANGM_665", "locus": "GCA013368015_00652" }, { "type": "cds", "sequence": "contig_1", "start": 372109, "stop": 372246, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCTTATCAGCAAGAAACTTACCAAATGTAACTGTAACAAAAGTAACTAGTTTATCAGTTGAATCTTTATTGGCAGCAGATGTTTTAGTTATTAGTTCAGATGATGTTAAATACTTAGAAGGATTGGTTAAATAA", "aa": "MFLSARNLPNVTVTKVTSLSVESLLAADVLVISSDDVKYLEGLVK", "aa_hexdigest": "8430c512fd0c8253469c444377393a46", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.5984, "isoelectric_point": 4.8610639572143555 }, "id": "LMJBGEANGM_666", "locus": "GCA013368015_00653" }, { "type": "cds", "sequence": "contig_1", "start": 372892, "stop": 373077, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514", "UniRef:UniRef90_UPI001314D514" ], "nt": "ATGGCAACAAAAAATGTAACATTAGAAAAATTCTACATTTCTGAAAAATTCGATGAAAATCGTAATATTTCATTTAATTTAAAAAAGGCTAACCAAAAATCATTCAAGAATTTTGAAAATTTTTCAGAAGCATTAACAGAATTTATTGAACTTTCTGAAAAATCAAAAAACAATACTAGAGTATGA", "aa": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRV", "aa_hexdigest": "65cd46d9cd54cbf3847c176a252340c8", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 372780, "stop": 373077, "observations": { "insertion": [ 372813 ], "mutation": [ 372894 ], "stop": [ 372894 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314D514", "query_cov": 1.0, "subject_cov": 0.6224489795918368, "identity": 1.0, "score": 115.0, "evalue": 3.69e-32, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRVWFHRDGAYRGSVGLEKAKLILEKVTGEKLKMKKQSTT" }, "paralog": false, "identity": 0.9897959183673469, "score": 184.0, "evalue": 5.18e-62, "description": "Internal stop codon at 372894. Frameshift due to insertion around 372813. Nonsense mutation around 372894.", "psc": { "uniref90_id": "UniRef90_UPI001314D514", "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314D514", "UniRef:UniRef50_UPI001314D514" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7242.053499999998, "isoelectric_point": 9.04905643463135 }, "id": "LMJBGEANGM_667", "locus": "GCA013368015_00654" }, { "type": "cds", "sequence": "contig_1", "start": 373077, "stop": 373403, "strand": "-", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGCCAGGACATTTAGGAGCTGAACAAGTAACTGTTCAAAACCTAGAAGTTGTTAAAGAAGATGCAGAAAACAATATTTTATTGATCAAGGGATCAGTTCCTGGTGCTAAAGGAGCATTCTTAATCATTAAAACTGCTAAGAAATCAATTAAGAATAAACCAGTTATTAAATTATTGAATCTAAAAGAAGCTTTGGCTAAAAATGAAATCTTTACTCAAGCTAAAAAATACAATCTTGAATTAAACATGGATATGTCATTAAAAGAAATGAAAGCTGCTTTGGATGAAGCAATTGCTAAGGCCGGAGAAGAAGGAGAAAATAAATAA", "aa": "MPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK", "aa_hexdigest": "8579bc1a82bf19d1b9aee04b89886a80", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 373077, "stop": 373703, "observations": { "mutation": [ 373418, 373517 ], "stop": [ 373418, 373517 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.40298507462686567, "identity": 1.0, "score": 201.0, "evalue": 3.99e-63, "gene_start": 1, "gene_end": 108, "reference_start": 161, "reference_end": 268, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9903846153846154, "score": 400.0, "evalue": 8e-144, "description": "Internal stop codon at 373418, 373517. Nonsense mutation around 373418, 373517.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "LMJBGEANGM_668", "locus": "GCA013368015_00655" }, { "type": "cds", "sequence": "contig_1", "start": 373515, "stop": 373883, "strand": "-", "frame": 1, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGAAAGGAATCTTAGGAAGAAAAGTTGGGATGACTCAACTATTTACTTCAGAAGGTAAATTAATACCTGTGACAGTAGTAGAAGTTAAACCAAATGTTGTTACTAAGGTTTTAACATCAGAAAAAAATGGTTATGTTGCTCTTCAATTAGCAACAGAAGATAAAAAATCTTCAAGAATCATTAAACCTGAAGTTAATTACTTTAAACAAGCAAATACAACACCTAAGCGCTTCGTAAAAGAAATCCGTGATATGTCAGGATTTTCATTAGGTGATACTGTAGATGCTTCATTATTTACCGCTGGAGAAGTTGTTGACGTAACAGCTATTTCTAAAGGTAAAGGATTTGCGGGTACCATCAAGAGATGA", "aa": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKR", "aa_hexdigest": "565ff623fc584ade78acfc33a3752bd4", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 373212, "stop": 373883, "observations": { "mutation": [ 373418, 373517 ], "stop": [ 373418, 373517 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.4552238805970149, "identity": 1.0, "score": 233.0, "evalue": 1.81e-75, "gene_start": 1, "gene_end": 122, "reference_start": 1, "reference_end": 122, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9910313901345291, "score": 427.0, "evalue": 2.16e-154, "description": "Internal stop codon at 373418, 373517. Nonsense mutation around 373418, 373517.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "LMJBGEANGM_669", "locus": "GCA013368015_00656" }, { "type": "cds", "sequence": "contig_1", "start": 373956, "stop": 374261, "strand": "-", "frame": 1, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_036433330.1", "SO:0001217", "UniParc:UPI00051394B6", "UniRef:UniRef100_D1J887", "UniRef:UniRef50_D1J887", "UniRef:UniRef90_D1J887" ], "nt": "ATGAAACTAGAAGTCAAAATTAAATCTTTTGATGCCCACTTAGTAGATGAAGCTGCTAAAAAAATAGTTTTATTAGCTCAAGCTGAAAAAGCAACTATTAGTGGTCCAATTCCTTTACCAACACATAAAGAAATTATTACCATCTTAAGATCTGTTCACGTTAACAAGAAATCTCGTGAACAATTTGAAAGCAGAACTCATAAACGTTTAATCGTTTTAGATAAAGTTTCTGAATCATTAGTTGATAAGGCTAAGAGATTAGATTTATCTGCTGGTGTACAAATAAGTTTAAAAACTGTTAACTAA", "aa": "MKLEVKIKSFDAHLVDEAAKKIVLLAQAEKATISGPIPLPTHKEIITILRSVHVNKKSREQFESRTHKRLIVLDKVSESLVDKAKRLDLSAGVQISLKTVN", "aa_hexdigest": "9470bae5d09f986c9e0abb6714a441dc", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00051394B6", "ncbi_nrp_id": "WP_036433330.1", "uniref100_id": "UniRef100_D1J887", "db_xrefs": [ "SO:0001217", "UniParc:UPI00051394B6", "RefSeq:WP_036433330.1", "UniRef:UniRef100_D1J887" ] }, "ips": { "uniref100_id": "UniRef100_D1J887", "uniref90_id": "UniRef90_D1J887" }, "psc": { "uniref90_id": "UniRef90_D1J887", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_D1J887", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_D1J887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J887" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "LMJBGEANGM_670", "locus": "GCA013368015_00657" }, { "type": "cds", "sequence": "contig_1", "start": 375990, "stop": 376355, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAATCAAAAAGAATTTTATTAACTATAGGGGCAGTAATACCTTTATTAGCCACAATACCATTAGTTTCATCAGGTTGCATAGAAACAAAAAAACCCGAAGTTAAACCAAATGAGCCAAAGAAACCAGAAAATAAAACATCGGGTTCAGATTCAGGTTTAATAAATCCTAATCCAAATCCTTCAAATCCAACTCCAGCTCCAATTCCTATTCCAGAAACTAAACCAGAAGAAAAAGAAGGTTATGTTGTTTATAAATCAACAAATGAAGATTATTTAAAAAAGAATAACATTTATTACTTTAGCAAATTACTTTATGACCCTGTGGATTTATCTGATGAGGATTATAACAATCATGAATGA", "aa": "MKKSKRILLTIGAVIPLLATIPLVSSGCIETKKPEVKPNEPKKPENKTSGSDSGLINPNPNPSNPTPAPIPIPETKPEEKEGYVVYKSTNEDYLKKNNIYYFSKLLYDPVDLSDEDYNNHE", "aa_hexdigest": "b1ba32444c970a734f70d964a997e8d1", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13499.17099999999, "isoelectric_point": 5.8980913162231445 }, "id": "LMJBGEANGM_671", "locus": "GCA013368015_00658" }, { "type": "cds", "sequence": "contig_1", "start": 376590, "stop": 376709, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTTTTAGATGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAAAAAAAGATCATGATTGA", "aa": "MRFLDEMDDYLDPDIAFKGLTMDETHINYVTENLKKDHD", "aa_hexdigest": "209942eba70162e7fb033debb5bf9d5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4690.1576000000005, "isoelectric_point": 4.335758018493654 }, "id": "LMJBGEANGM_672", "locus": "GCA013368015_00659" }, { "type": "cds", "sequence": "contig_1", "start": 376710, "stop": 376856, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992", "UniRef:UniRef90_UPI001E37B992" ], "nt": "ATGAATAAAAAACTTATTTACTCTGCTGATTCAAAAATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGA", "aa": "MNKKLIYSADSKMPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEG", "aa_hexdigest": "c4d1d168f1c28f6b5951d1b58b1578ee", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 376710, "stop": 376977, "observations": { "insertion": [ 376896 ], "mutation": [ 376854 ], "stop": [ 376854, 376921 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E37B992", "query_cov": 1.0, "subject_cov": 0.5517241379310345, "identity": 0.8959999999999999, "score": 85.9, "evalue": 5.97e-21, "gene_start": 1, "gene_end": 48, "reference_start": 1, "reference_end": 47, "reference_sequence": "MNKNLFTHDSKMPSTFIRDEFNHQSIEDMDGFVKELISDYENAMYEGWTVENKANDWFVAPFHLDLILQWEINFHNKLMICILIKMV" }, "paralog": false, "identity": 0.875, "score": 148.0, "evalue": 3.99e-48, "description": "Internal stop codon at 376854, 376921. Frameshift due to insertion around 376896. Nonsense mutation around 376854.", "psc": { "uniref90_id": "UniRef90_UPI001E37B992", "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E37B992", "UniRef:UniRef50_UPI001E37B992" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E37B992", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E37B992" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5639.2391, "isoelectric_point": 4.450743293762207 }, "id": "LMJBGEANGM_673", "locus": "GCA013368015_00660" }, { "type": "cds", "sequence": "contig_1", "start": 376920, "stop": 377102, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTTCCACAAGAAACTTACAATATGTACACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAGATTAAATTTTGAAGTTTATAAGAATGTAATTCAATGA", "aa": "MRNKFPQETYNMYTDKNGLDKTTPTPAYNNYNPVAFNFLVMERFDLSERLNFEVYKNVIQ", "aa_hexdigest": "f68114f5a21bf0351db6812d629b88f9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7232.061299999999, "isoelectric_point": 6.107997703552246 }, "id": "LMJBGEANGM_674", "locus": "GCA013368015_00661" }, { "type": "cds", "sequence": "contig_1", "start": 377316, "stop": 377408, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1", "UniRef:UniRef90_UPI001E44B7D1" ], "nt": "ATGGGCGACAAAATTGACTTTGATTACACTTATAGATTTGCATATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGA", "aa": "MGDKIDFDYTYRFAYKFLYEKLVPTMVANG", "aa_hexdigest": "aec741b8d9ba2cf80992a7ddf680f4ca", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 377301, "stop": 377462, "observations": { "mutation": [ 377406 ], "stop": [ 377406 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E44B7D1", "query_cov": 1.0, "subject_cov": 0.5660377358490566, "identity": 1.0, "score": 65.9, "evalue": 9.65e-14, "gene_start": 1, "gene_end": 30, "reference_start": 6, "reference_end": 35, "reference_sequence": "MKSLTMGDKIDFDYTYRFAYKFLYEKLVPTMVANGWNMPGEFDVLEKNMLDEK" }, "paralog": false, "identity": 0.8679245283018868, "score": 94.4, "evalue": 1.01e-27, "description": "Internal stop codon at 377406. Nonsense mutation around 377406.", "psc": { "uniref90_id": "UniRef90_UPI001E44B7D1", "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E44B7D1", "UniRef:UniRef50_UPI001E44B7D1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3597.119100000001, "isoelectric_point": 5.918780708312987 }, "id": "LMJBGEANGM_675", "locus": "GCA013368015_00662" }, { "type": "cds", "sequence": "contig_1", "start": 377412, "stop": 377591, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAACTGAATTTGATATTAATCTAGAAAGGAGTATGCTAGATAAAAATAGAAAAGATTCATATATTTATGACTATATCTTTAAATATTTAAATGTCATTTTTAAAGCTAAATACAAAGACATTGATGAAATTAAAAAACCAAAAGGATTTTTAGATTTAAATAATAAAGAACATTGA", "aa": "MPTEFDINLERSMLDKNRKDSYIYDYIFKYLNVIFKAKYKDIDEIKKPKGFLDLNNKEH", "aa_hexdigest": "7db9c1d35d9c328901fe585a769b7df0", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7250.265700000002, "isoelectric_point": 7.914606285095215 }, "id": "LMJBGEANGM_676", "locus": "GCA013368015_00663" }, { "type": "cds", "sequence": "contig_1", "start": 377790, "stop": 377897, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTGTATTTATATACAAATTCTTTGTCCAGATATGATTAAAAACAATTTAATGGGTAAAGAATCAGAAGGATATATTTACTTAGATCTTTCGGATGCTGATTAA", "aa": "MPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDAD", "aa_hexdigest": "9511f5b3800971f3e26ddc174191068a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.6492000000017, "isoelectric_point": 4.1011266708374015 }, "id": "LMJBGEANGM_677", "locus": "GCA013368015_00664" }, { "type": "cds", "sequence": "contig_1", "start": 377946, "stop": 378590, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J888", "UniRef:UniRef90_D1J888" ], "nt": "ATGAAAAAAAATATTTTATTAAGTTTTATTTTTCCAGCTGTGTCATTATTACCTCTTTTAAGCATATCTTGCAAGGAAACTACCACAATTAATAATACCTTAGATACAAGTAATAACACTATTGATATAGATAATAGTGGCACTACAGGTACTAATGACAAAAATACAGATAGCACAAATACTGGTACTACTGATACTGATAATAAGAATATAGGTAATTCTAGTGTTGGAACTGCAGATACTAATGACAAAAATACAGACAGCACAAATACCAGTACCGCCGATACTAACGACAAGAAAGATGACAATAAAGATACAAATAACGAAAATACTGATGATACAGATCCCGTACAACCCGATAGACTTCATAGAATGTTTCAAAGAAAGCTATTATCCGCTTCTCAAATTTCAGAAATAACAACACAATTTTCATTTGAACTTTCCAAAGAAGGCAAAAAATTTGTAGCCAATAATGGAGCCAAAGCCTTAATTGAAATTGTTAATAAAGCAATTGATGAAACTAAAAAATACGATACTATTGAAATGTTAAAAAGTCAAGAAAGAATTGCTTATACATATTTTATGGAAGCTAATGACATTAAAAAATATTTTGATATAAAAATTCCTGAAAATCTTATGCCTTGA", "aa": "MKKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTSNNTIDIDNSGTTGTNDKNTDSTNTGTTDTDNKNIGNSSVGTADTNDKNTDSTNTSTADTNDKKDDNKDTNNENTDDTDPVQPDRLHRMFQRKLLSASQISEITTQFSFELSKEGKKFVANNGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMP", "aa_hexdigest": "f4c59eb00be1a5fb52fd9536ee1e1aa6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 377790, "stop": 378584, "observations": { "mutation": [ 377948 ], "stop": [ 377948 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J888", "query_cov": 0.9906542056074766, "subject_cov": 0.7881040892193308, "identity": 0.92, "score": 245.0, "evalue": 1.4e-78, "gene_start": 3, "gene_end": 214, "reference_start": 6, "reference_end": 217, "reference_sequence": "MKGIWKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTNNNTIDIDNSGTTDTNDKNTNSTNTGTTDTDNKNIGNSSVDTTDTNDKNTDSTNTGTTDTSDKKDDNKDTNNENTDDTDPEQPNRLHRIFQRKLLSASQISEITTQFSFELSKEGKKFVANKGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMPWGHKLLIEFLKKGEEKNMPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDVD" }, "paralog": false, "identity": 0.928030303030303, "score": 358.0, "evalue": 7.17e-126, "description": "Internal stop codon at 377948. Nonsense mutation around 377948.", "psc": { "uniref90_id": "UniRef90_D1J888", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J888", "UniRef:UniRef50_D1J888" ] }, "pscc": { "uniref50_id": "UniRef50_D1J888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J888" ], "product": "Variable surface lipoprotein" } }, "id": "LMJBGEANGM_678", "locus": "GCA013368015_00665" }, { "type": "cds", "sequence": "contig_1", "start": 379023, "stop": 379340, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATATTGAAGGTCCCGGACATGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCTGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCACCCGAAACACCTGGTTCAATTCACGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGTGAAGCAGAAACAGGCCCACTAGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMISNSYLDGSYSEIYPEISQDEAGLAKMFKRFSFPGGTASHAAPETPGSIHEGGELGYSISHGTGAILDNPDVICAAVVGDGEAETGPLATS", "aa_hexdigest": "cb9a70a1207895298182aa6f7a6c75c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.721599999992, "isoelectric_point": 4.3166601181030275 }, "id": "LMJBGEANGM_679", "locus": "GCA013368015_00666" }, { "type": "cds", "sequence": "contig_1", "start": 379211, "stop": 379531, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAGATTACTTTTATCTTCGCTTCATTCAACGAAAATTGGATTTCAGCCTAAACCTTCAAAGTATTTTTTAATTTCTTCTTTTGGTTTTCTTGAAAGAAGTGTAGGGTTTGAAATTTTTCCACCATTCAAATGTAATATAGGAAGTATTGCTCCATCATTTACAGGGTTAATAAAAGCATTTGAGAATCAGCTAGTTGCTAGTGGGCCTGTTTCTGCTTCACCATCGCCAACAACAGCTGCACAAATAACATCAGGGTTATCTAATATTGCTCCTGTTCCATGGCTTATAGAATATCCTAATTCACCACCTTCGTGA", "aa": "MSRLLLSSLHSTKIGFQPKPSKYFLISSFGFLERSVGFEIFPPFKCNIGSIAPSFTGLIKAFENQLVASGPVSASPSPTTAAQITSGLSNIAPVPWLIEYPNSPPS", "aa_hexdigest": "e74dad0e4e0293078566f4399dcc17f9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11315.932199999985, "isoelectric_point": 9.359794044494631 }, "id": "LMJBGEANGM_680", "locus": "GCA013368015_00667" }, { "type": "cds", "sequence": "contig_1", "start": 379542, "stop": 379637, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATCATTAGATAAGGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCTGCTGAAGAAGCAACAAGACCAACTTGA", "aa": "MAKSLDKAIESIKEIQAEARKKPAEEATRPT", "aa_hexdigest": "9dabdcbb4dcd8d8b3e81f6139aafeee5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3440.9214000000006, "isoelectric_point": 8.141083717346195 }, "id": "LMJBGEANGM_681", "locus": "GCA013368015_00668" }, { "type": "cds", "sequence": "contig_1", "start": 379989, "stop": 380117, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGA", "aa": "MAEMAKFAADIMKDNPSSFRVFGPDETKSNRMFALFNVTNRQ", "aa_hexdigest": "7538684bd81affb55bcf87471adf19fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4814.4602, "isoelectric_point": 8.253452110290528 }, "id": "LMJBGEANGM_682", "locus": "GCA013368015_00669" }, { "type": "cds", "sequence": "contig_1", "start": 380505, "stop": 380633, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAGCCTTAACCGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGCTGAGGATGCCGAAGAATTACTAGAAAAAGGTTATAAAGTTGTTCCTTGA", "aa": "MNKALTERNVINLIVASKQPREQFFTAEDAEELLEKGYKVVP", "aa_hexdigest": "ecc07d94a51c28783c06d4ee48e386df", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4820.4783, "isoelectric_point": 5.189195442199705 }, "id": "LMJBGEANGM_683", "locus": "GCA013368015_00670" }, { "type": "cds", "sequence": "contig_1", "start": 380709, "stop": 381149, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTTTATGTTCTTGACTTATTAAAATTGAGATCTAGAAAAATAGATCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGATTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGTGATATTACAACATCATTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAATTAGACCAAAAACTAGAATATCATAGAAACTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGA", "aa": "MAAISLINQEYPHLKIRYVYVLDLLKLRSRKIDPRGISDEEFDKVFTKNKPIIFAFHGFEGLLRDIFFTRSNHNLIAHGYRENGDITTSFDIRQLSEMDRYHIAKDAAEAVYGKDAKAFMNKLDQKLEYHRNYIDEYGYDMPEVVE", "aa_hexdigest": "59f58ddc36c55ba5c33ce12997ad2aed", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 0.9863013698630136, "subject_cov": 0.8674698795180723, "identity": 0.59, "score": 189.0, "evalue": 3.88e-59, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17242.383899999986, "isoelectric_point": 6.071961784362792 }, "id": "LMJBGEANGM_684", "locus": "GCA013368015_00671" }, { "type": "cds", "sequence": "contig_1", "start": 381256, "stop": 381471, "strand": "+", "frame": 1, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855578.1", "SO:0001217", "UniParc:UPI0001BC22A9", "UniRef:UniRef100_D1J890", "UniRef:UniRef50_Q6KIG2", "UniRef:UniRef90_D1J890" ], "nt": "ATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAA", "aa": "MQKDIHPKYNQIKAVCSSCNSTIEFGSTADKVTLDVCSNCHAFYTGNRTDVKARGRVERFNKILEKSHKKN", "aa_hexdigest": "14ec0f3da0d8b604325a5ed87f9f7f2a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A9", "ncbi_nrp_id": "WP_012855578.1", "uniref100_id": "UniRef100_D1J890", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A9", "RefSeq:WP_012855578.1", "UniRef:UniRef100_D1J890" ] }, "ips": { "uniref100_id": "UniRef100_D1J890", "uniref90_id": "UniRef90_D1J890" }, "psc": { "uniref90_id": "UniRef90_D1J890", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_Q6KIG2", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG2" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "LMJBGEANGM_685", "locus": "GCA013368015_00672" }, { "type": "cds", "sequence": "contig_1", "start": 381567, "stop": 381764, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891", "UniRef:UniRef90_D1J891" ], "nt": "ATGAATATAAATTTCAAACTAATGAGCGAAGAAGAAACAAAAAATATTGTAGGAGGTTTTGCAATTACTGCTTTAATTTCAACTATTTTGTCTGTAGTAACAAGCGCTGTCGGAATTATTGGAACTTCAATAGGCTTGGCTAAGACTTTAAATGCAAATAAAGGAGAAGTAAAAGCCGGGAATATTTCAATGAAATGA", "aa": "MNINFKLMSEEETKNIVGGFAITALISTILSVVTSAVGIIGTSIGLAKTLNANKGEVKAGNISMK", "aa_hexdigest": "065e6177c50b072b4c85f6b419cfdbac", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 381513, "stop": 381806, "observations": { "mutation": [ 381569 ], "stop": [ 381569 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J891", "query_cov": 1.0, "subject_cov": 0.6701030927835051, "identity": 0.985, "score": 114.0, "evalue": 8.57e-32, "gene_start": 1, "gene_end": 65, "reference_start": 15, "reference_end": 79, "reference_sequence": "MEYLLYLKLKGEIKMNINFKLMSEEETKNIVGGFAITALISTILSVVTSTVGIIGTSIGLAKTLNANKGEVKAGNISMKWDNSNENIGYAVGMHYCT" }, "paralog": false, "identity": 0.979381443298969, "score": 185.0, "evalue": 2.85e-62, "description": "Internal stop codon at 381569. Nonsense mutation around 381569.", "psc": { "uniref90_id": "UniRef90_D1J891", "uniref50_id": "UniRef50_D1J891", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J891", "UniRef:UniRef50_D1J891" ] }, "pscc": { "uniref50_id": "UniRef50_D1J891", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6710.831400000001, "isoelectric_point": 9.303642082214353 }, "id": "LMJBGEANGM_686", "locus": "GCA013368015_00673" }, { "type": "cds", "sequence": "contig_1", "start": 383215, "stop": 383631, "strand": "-", "frame": 3, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4", "UniRef:UniRef90_UPI002FE0DEE4" ], "nt": "ATGCTTGGTGGAATTAGTGACTTTGATTTTAAAGAAATTACGCCAACTTCAATTCATGCTTTAATGTTAGAAAAATTGCATAATCTAGAAGTAAATATATTATTGAATTACGAACAATATAGATTTGTTAATGTTATTAAAGATATTAACAATTTTGTTATTGATTTAAGTAGTTACTATATTTCTATAACCAAAGATATTTTATATCTTGAAAAACAAGATGATCCTGAACGTAGAATGGTTCAATATGTTTTTAGCAACATTATTGAGGTCTTATTAAAAGCTCTTGCCCCAATATTGCCAACTACAACCGAAGAAATTTATAAATATTACAATAAACCTAACAAGTTGCCTTCTGTTCATCTATTGTCATTTTTAGAAAGAAAAGATTTTGATTTTAAATTACAAGATCAATGA", "aa": "MLGGISDFDFKEITPTSIHALMLEKLHNLEVNILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLSFLERKDFDFKLQDQ", "aa_hexdigest": "51e99190661892822af4c57c202c293a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 383094, "stop": 383622, "observations": { "insertion": [ 383115 ], "mutation": [ 383127, 383217 ], "stop": [ 383127, 383217 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "query_cov": 0.9782608695652174, "subject_cov": 0.7627118644067796, "identity": 0.985, "score": 266.0, "evalue": 1.42e-89, "gene_start": 4, "gene_end": 138, "reference_start": 3, "reference_end": 137, "reference_sequence": "MLGISDFDFKEITPTSIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLSFLERKDFDFKFQDQWKEFFEIKDEVYKLIEEKIKSQAIKRPNEAWVVIKAIQTL" }, "paralog": false, "identity": 0.9771428571428571, "score": 329.0, "evalue": 9.33e-117, "description": "Internal stop codon at 383127, 383217. Frameshift due to insertion around 383115. Nonsense mutation around 383127, 383217.", "psc": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE0DEE4", "UniRef:UniRef50_UPI002FE0DEE4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4" ], "product": "class I tRNA ligase family protein" } }, "id": "LMJBGEANGM_687", "locus": "GCA013368015_00674" }, { "type": "cds", "sequence": "contig_1", "start": 383731, "stop": 383850, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBK0", "UniRef:UniRef90_A0A432FBK0" ], "nt": "TTGGTTTCTCATGGGTTTGTTTTAGATGGCAAAGGTCAAAAAATGTCAAAATCAAAAGGCAATGTTGTTGATCCTTTAGCTGTAATTGATAAATATGGAGCAGATATTTTAAGACTTTGA", "aa": "MVSHGFVLDGKGQKMSKSKGNVVDPLAVIDKYGADILRL", "aa_hexdigest": "3e3c4202b6a38a44c5865b9dd4e3e816", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 383731, "stop": 383892, "observations": { "mutation": [ 383877 ], "stop": [ 383877 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A432FBK0", "query_cov": 1.0, "subject_cov": 0.7358490566037735, "identity": 0.846, "score": 70.5, "evalue": 2.13e-15, "gene_start": 1, "gene_end": 39, "reference_start": 15, "reference_end": 53, "reference_sequence": "MNSIAYRGKSPYKNLVSHGFVLDGKNQKMSKSKGNVVDPLKIISKQGADILRL" }, "paralog": false, "identity": 0.7735849056603774, "score": 83.6, "evalue": 1.78e-23, "description": "Internal stop codon at 383877. Nonsense mutation around 383877.", "psc": { "uniref90_id": "UniRef90_A0A432FBK0", "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "uniref50_id": "UniRef50_A0A432FBK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A432FBK0", "UniRef:UniRef50_A0A432FBK0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBK0" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" } }, "id": "LMJBGEANGM_688", "locus": "GCA013368015_00675" }, { "type": "cds", "sequence": "contig_1", "start": 384379, "stop": 384657, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCAGTTGTTTTTGGTCATCATGTTAGTTTAGATACTGGTACAGGTTTAGTTCACATTGCCCCATTATTTGGTGAAGATGACTTTATTATTGGCAAAAAAGAAAACCTAGAAATGATTATGCACATAAACGATGATGGAACAATCAATGATAAAGGCTTTGAATATAGCGGTGTTTTTTACGATGATGCAAATCCTTTAATTGGCAAATTTTTAGCAGAAAATAATCAATTAATTGCGTTTAAGAAAATTAAACACTCATATCCTCATGATTGA", "aa": "MGPVVFGHHVSLDTGTGLVHIAPLFGEDDFIIGKKENLEMIMHINDDGTINDKGFEYSGVFYDDANPLIGKFLAENNQLIAFKKIKHSYPHD", "aa_hexdigest": "ec0cfcc482567d5921c86253b942139e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10259.48839999999, "isoelectric_point": 5.131447029113769 }, "id": "LMJBGEANGM_689", "locus": "GCA013368015_00676" }, { "type": "cds", "sequence": "contig_1", "start": 384736, "stop": 384858, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGCTAACTCTGGCGTTGCAATTAACGATCAATTCGAATATGCAAAAGTGTTAGTAAATGGCAATAAATATATAATTGCAAAAGATTTAGTTGAATCAGTTGCTGCAGTTGCTAAATGA", "aa": "MLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAK", "aa_hexdigest": "31f4ca199c84f3b0ae8203bebb5441e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4267.897200000002, "isoelectric_point": 5.970674705505371 }, "id": "LMJBGEANGM_690", "locus": "GCA013368015_00677" }, { "type": "cds", "sequence": "contig_1", "start": 385012, "stop": 385275, "strand": "-", "frame": 3, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25", "UniRef:UniRef90_UPI0023AA8A25" ], "nt": "TTGCCTATTGAACATAAAATGTTGCTAGAAGCTAAAAAGAACGCTAAAGATTTTAGTGTTATTGAATTAAGGAAGAAGGCAGCAGAATATGCTTTAAGTCAAGTTGAACATCAAAAGGCTCAATTTAAAAAATTAAGTTTATTGACAGACTTTTCAGAAATTTATGTGACATTGGATAAAAACCTAGAATTTAAACAATTAGAACTTTTTGCTAAAATGGTTAAAGAAGGCTTAATTTATCGCGATTTAAAACCAGTTTATTGA", "aa": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLLTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVY", "aa_hexdigest": "3a4646ba2f4069d42af0db44b32f3f27", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 384709, "stop": 385275, "observations": { "mutation": [ 384738, 384864, 384879, 385014 ], "stop": [ 384738, 384864, 384879, 385014 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AA8A25", "query_cov": 1.0, "subject_cov": 0.4484536082474227, "identity": 0.9890000000000001, "score": 166.0, "evalue": 1.62e-50, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLFTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVYWSPSSQSALAEAEVEYADHVSPSLYVAFKVIEGNKYVENGDYLIIWTTTPWTLLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAKWEEYQVLSTFKGKEF" }, "paralog": false, "identity": 0.9627659574468085, "score": 345.0, "evalue": 1.05e-123, "description": "Internal stop codon at 384738, 384864, 384879, 385014. Nonsense mutation around 384738, 384864, 384879, 385014.", "psc": { "uniref90_id": "UniRef90_UPI0023AA8A25", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AA8A25", "UniRef:UniRef50_UPI0023AA8A25" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25" ], "product": "class I tRNA ligase family protein" } }, "id": "LMJBGEANGM_691", "locus": "GCA013368015_00678" }, { "type": "cds", "sequence": "contig_1", "start": 385288, "stop": 385581, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "nt": "ATGGAAGAAAAGAAAGATTATAAAGATACGTTATTGATGCCAAATACTGATTTTCCAATGAAAGCAAATTTAGTTGAAAAAGAATTGGAATATCAAAAACAATGGCTAGATATTAAGTTGTATCAAACTATTTTAAATAGAAATAAGAACAGTCAATCATTTGTATTACATGATGGTCCTCCATATGCAAATGGCAATATTCATGTTGGACACGCTCTAAACAAAATTTTAAAAGATATAATTGTAAGATTTAGAAGTATGCAGGGATATTATTCTCCATATGTTCCGGGTTGA", "aa": "MEEKKDYKDTLLMPNTDFPMKANLVEKELEYQKQWLDIKLYQTILNRNKNSQSFVLHDGPPYANGNIHVGHALNKILKDIIVRFRSMQGYYSPYVPG", "aa_hexdigest": "68b231c91ac2e4767637ea05b37ce65a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00190B9126", "query_cov": 0.9381443298969072, "subject_cov": 0.9891304347826086, "identity": 0.626, "score": 129.0, "evalue": 2.2e-37, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A352TTR4" }, "pscc": { "uniref50_id": "UniRef50_A0A352TTR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_692", "locus": "GCA013368015_00679" }, { "type": "cds", "sequence": "contig_1", "start": 385825, "stop": 386040, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGATTTTAGAAGTTAGCGAAAATATTGAAAAAACTAAGTTTAATATTGCAATAAGCAAAATGATGGTGTTTACTAATTACTTAACATCTTTAAAAACTATTCCATCATCATTTGTTTTGAAACAATTTGCTATTTTATTAAGTCCGTTTGCGCCATTTTTAGCTGAAGAACTTTTGTTTATGTTGAATGAAAAACCAATTCAATATCAAACTTGA", "aa": "MILEVSENIEKTKFNIAISKMMVFTNYLTSLKTIPSSFVLKQFAILLSPFAPFLAEELLFMLNEKPIQYQT", "aa_hexdigest": "b5d80819d9f4ac2c827be821519144af", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 85, "aa_cov": 0.9014084507042254, "hmm_cov": 1.0, "evalue": 1.8347961275971776e-10, "score": 36.3955192565918, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 8205.705999999995, "isoelectric_point": 6.140395927429199 }, "id": "LMJBGEANGM_693", "locus": "GCA013368015_00680" }, { "type": "cds", "sequence": "contig_1", "start": 386167, "stop": 386361, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGGTATTGTTAATACAAAAGAACCATTTACAAAGCTAATAAACCAAGGATTAATTATGGGACCTGATGGTCAAAAAATGTCTAAATCTCTAGGCAACGTTATAAATCCTGATGATTTTATTTTATCTCATGGAGCAGATAGTTTAAGAGTTTATGAAATGTTTATGGGTCCTTTGGTGGAATCAAAATCTTGA", "aa": "MGIVNTKEPFTKLINQGLIMGPDGQKMSKSLGNVINPDDFILSHGADSLRVYEMFMGPLVESKS", "aa_hexdigest": "7700a8b03e05cbedaece89c5a097eac2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 48, "aa_cov": 0.75, "hmm_cov": 1.0, "evalue": 7.774391667590817e-16, "score": 53.310489654541016, "start": 5, "stop": 52 } ], "seq_stats": { "molecular_weight": 6998.0638, "isoelectric_point": 5.587352561950683 }, "id": "LMJBGEANGM_694", "locus": "GCA013368015_00681" }, { "type": "cds", "sequence": "contig_1", "start": 386611, "stop": 387024, "strand": "-", "frame": 3, "gene": null, "product": "leucine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W1XXR9" ], "nt": "ATGTCATATGGAAGTGGAGCAGTAATGTGTGTGCCCGGCCATGATCAAAGAGATTATGAATTTGCTAAAAAATTTGATTTAAAAATTAAAAAAATTATTGAATGTGAAGAATTACCGTTTTTAGGAGATGGTATTCATATCAATTCAGATATTGCAAATGGTTTAGATAATGCAGGCGCAACAAAATGTATTTTCAATGAATTGAAAGCAAATAATTTAGCAGATGAAAAGGTAAGTTATAAATTACGCGATTGGATTTTTTCAAGGCAAAGATATTGGGGAGAACCATTTCCTGTTCTATTTGATGAAGAAAATAATATTTATCTAGTAAAAGAATTAGTTGAATTGCCAAAGATAAAAAACATAAAACCATCAGGTACAACCTCTGGTCCGCTGGCAAATGTTGAAAGCTGA", "aa": "MSYGSGAVMCVPGHDQRDYEFAKKFDLKIKKIIECEELPFLGDGIHINSDIANGLDNAGATKCIFNELKANNLADEKVSYKLRDWIFSRQRYWGEPFPVLFDEENNIYLVKELVELPKIKNIKPSGTTSGPLANVES", "aa_hexdigest": "25efde7ec658547386c1a0849572faae", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030DD5651", "query_cov": 0.8248175182481752, "subject_cov": 0.9912280701754386, "identity": 0.522, "score": 115.0, "evalue": 8.25e-31, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_W1XXR9" }, "pscc": { "uniref50_id": "UniRef50_W1XXR9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_W1XXR9" ], "product": "leucine--tRNA ligase" }, "genes": [], "id": "LMJBGEANGM_695", "locus": "GCA013368015_00682" }, { "type": "cds", "sequence": "contig_1", "start": 387043, "stop": 387291, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGCAACTCTCAACATTTACTACTAGATTGGATACTATCTATGGTGTAACTGCAATAGTAATTTCTCCCGAACACCCACTTCTTCAAAAAATAACTACTACAAAATATCATGATGATATTTCTAACTATTTAAAAGAAGTTAAATTAAAAAATGAATTAGAAAGAACATCTTTAAATAAAGACAAAACAGGATATTTTACTGGTTCGTATGCAATTCATCCATTGACTAATGAATTAATTCCTATCTGA", "aa": "MQLSTFTTRLDTIYGVTAIVISPEHPLLQKITTTKYHDDISNYLKEVKLKNELERTSLNKDKTGYFTGSYAIHPLTNELIPI", "aa_hexdigest": "a77237518fe7751e4b2060fb7557dea1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 81, "aa_cov": 0.9878048780487805, "hmm_cov": 0.9753086419753086, "evalue": 5.02736844478701e-24, "score": 80.32379913330078, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9383.642299999994, "isoelectric_point": 6.8211565017700195 }, "id": "LMJBGEANGM_696", "locus": "GCA013368015_00683" }, { "type": "cds", "sequence": "contig_1", "start": 387793, "stop": 387915, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTACCCTTCAGGAATGGGATTGCACGTTGGGCATTTAGAGGGTTATACCGCTACTGATATTATTGCTAGGTATAAACGCCTAAATGGTTTTGATGTGTTGCATCCTATTGGCTGA", "aa": "MFPYPSGMGLHVGHLEGYTATDIIARYKRLNGFDVLHPIG", "aa_hexdigest": "21af7fc6286f9ee63374d2bcb75ef168", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4445.087, "isoelectric_point": 6.810016059875489 }, "id": "LMJBGEANGM_697", "locus": "GCA013368015_00684" }, { "type": "cds", "sequence": "contig_1", "start": 388296, "stop": 389522, "strand": "+", "frame": 3, "gene": null, "product": "Lmp protein", "db_xrefs": [ "RefSeq:WP_047207682.1", "SO:0001217", "UniParc:UPI0006401743", "UniRef:UniRef100_UPI0006401743", "UniRef:UniRef50_A0A454CAH5", "UniRef:UniRef90_A0A454CAH5" ], "nt": "ATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAAACATTTAATAATTTAAAAAATAAATTGCAAGATTTATTGAATTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTACAAACACAACAATTGATGATAATTCTCAAATACAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCCTAATAAAACTAAGACAAGATTTCAACAAAGCAAAAGAAGATCTAAAAAATCTTTTGGAATCAAAAGAAGTAGCGGATATTGAAAAAGAGATCGAATTAAACACTCTAAATACCACTGATATTGCCAAAGATTCTTTACAACAAGAAATTCAAACCAAAATACAAAAAATTCAAGATGCTATACAATCAATAAACAAAAAAATCAATGACAAAAAGCTATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAAGACACTTGAAAATTTAATTGCTACAGAAGATGCTAAAGCAGTTGGAATTGATGAAGCAAAAAAAGCTCTTGACAATAATAAAAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAACGATTTGAACGATATTACAGCCAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAACTTTCAAAAAATCTAAGCAAGATTTAGAAGAATATATAAATAAAAATTTAAATGAAAATAAATTTATTAAAGTTAAAGAAAAAGCCCAAAAAACCATTGAAAAATTTTCTAATATAAACAAAGATAATTCCTCAATCAAAGAAATTGAAGATGCTACTAGCTCGATAAAAGAGGCTAAAGAAGTTGCTTTGAAAGAAGAAGAAGGAATCAAAAAATTAAATGAAGTCTTGGAATATGTCAATAAAAAAAATGAACTAGAAGAATTAATAAAATCATCTAATACTCAATCAAATGAAGATGCTATTAAAAAAGCCCAAAAAACTTTAGACGATAATAAAGCAACAAATACCGATGAATTATCTTCCAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAAAAGCAATAATTGAACTAAAGGCAGCAATAGAAGCAACAAAAAAATCTAAGAATTAA", "aa": "MKRKILLFTPLIIAGSLIPTTLTACNNEDSKLTKQLKAKQTFNNLKNKLQDLLNSEDAKDIDANKEINTLTNTTIDDNSQIQDIESKTKTIEDAIESLTKKINDKKILIKLRQDFNKAKEDLKNLLESKEVADIEKEIELNTLNTTDIAKDSLQQEIQTKIQKIQDAIQSINKKINDKKLLNKEQQNLVEAKKTLENLIATEDAKAVGIDEAKKALDNNKNNKISTIEEIKKAINDLNDITAKLNQKIANKKQELLETFKKSKQDLEEYINKNLNENKFIKVKEKAQKTIEKFSNINKDNSSIKEIEDATSSIKEAKEVALKEEEGIKKLNEVLEYVNKKNELEELIKSSNTQSNEDAIKKAQKTLDDNKATNTDELSSKDFEEKRKNIEKAIIELKAAIEATKKSKN", "aa_hexdigest": "2b88bbb69a43b7e52b17bac0565fb67b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0006401743", "ncbi_nrp_id": "WP_047207682.1", "uniref100_id": "UniRef100_UPI0006401743", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006401743", "RefSeq:WP_047207682.1", "UniRef:UniRef100_UPI0006401743" ] }, "ips": { "uniref100_id": "UniRef100_UPI0006401743", "uniref90_id": "UniRef90_A0A454CAH5" }, "psc": { "uniref90_id": "UniRef90_A0A454CAH5", "product": "Lmp protein", "uniref50_id": "UniRef50_A0A454CAH5" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAH5" ], "product": "Lmp protein" }, "genes": [], "id": "LMJBGEANGM_698", "locus": "GCA013368015_00685" }, { "type": "cds", "sequence": "contig_1", "start": 389860, "stop": 390348, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D52A", "UniRef:UniRef90_UPI002410D52A" ], "nt": "ATGGGTAAAAAAGCAAAATTATTTGTCTTTGGTGGGATTAGTTTTTTAGCAGGAGCTTCAATTATTGGTTCTGTTGCAGCATATGCTTCAATTAACAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTAGCAATTTAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACCTTAAGTGAAGTTGAAAAAATAGATAAAAATTCAGATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAACAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTAAATAAAGAAAGCCAAGGACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGA", "aa": "MGKKAKLFVFGGISFLAGASIIGSVAAYASINKKNEIRVSEYNKFLEEKTNLNSFSNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKMLLEKEKAFIQYNNVKKQLQDMLLKIFDFLNKESQGHYYIGDGNEKIINKQN", "aa_hexdigest": "457f08d36eb00d068700c9d12150d4fd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 389860, "stop": 390514, "observations": { "insertion": [ 390469 ], "mutation": [ 390346 ], "stop": [ 390346 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410D52A", "query_cov": 1.0, "subject_cov": 0.7465437788018433, "identity": 0.988, "score": 294.0, "evalue": 1.12e-99, "gene_start": 1, "gene_end": 162, "reference_start": 1, "reference_end": 162, "reference_sequence": "MGKKAKLFVFGGISFLVAASIIGSVAAYASINKKNEIRVSEYNKFLEEKTNLNSFSNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKMLLEKEKAFIQYNNVKKQLQDMLLKIFDFLNKESQGHYYIGDGNEKIINKQNWLLKEINENQKYFSNITIDSDIDSIKLATTKLVEIKFLINENKKHINLPKNITAY" }, "paralog": false, "identity": 0.967741935483871, "score": 401.0, "evalue": 3.47e-144, "description": "Internal stop codon at 390346. Frameshift due to insertion around 390469. Nonsense mutation around 390346.", "psc": { "uniref90_id": "UniRef90_UPI002410D52A", "uniref50_id": "UniRef50_UPI002410D52A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410D52A", "UniRef:UniRef50_UPI002410D52A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410D52A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D52A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18456.03129999999, "isoelectric_point": 9.483186531066895 }, "id": "LMJBGEANGM_699", "locus": "GCA013368015_00686" }, { "type": "cds", "sequence": "contig_1", "start": 390691, "stop": 390819, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATGACAAAACTGCACTTCTTGAGGAATTCGACATTGATTTTTTTGGATTCAAAATCAAAGATAAAAATTTCTATAAAAATTATGTTGAGTTGTTGACACCATATAAAAAGGCTATTGATTTGTGA", "aa": "MYDKTALLEEFDIDFFGFKIKDKNFYKNYVELLTPYKKAIDL", "aa_hexdigest": "06aa18affaf7abae4b9665d86555c85e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5128.885300000001, "isoelectric_point": 5.086430549621581 }, "id": "LMJBGEANGM_700", "locus": "GCA013368015_00687" }, { "type": "cds", "sequence": "contig_1", "start": 390883, "stop": 391113, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E", "UniRef:UniRef90_UPI0024112F2E" ], "nt": "TTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGATAAATCAACCTGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTATTCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAACGACAATCAATAA", "aa": "MYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLIPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ", "aa_hexdigest": "d360ff4b85445756d6d1efd4595df859", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 390787, "stop": 391113, "observations": { "mutation": [ 390817, 390874 ], "stop": [ 390817, 390874 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024112F2E", "query_cov": 1.0, "subject_cov": 0.7037037037037037, "identity": 0.9740000000000001, "score": 154.0, "evalue": 2.63e-47, "gene_start": 1, "gene_end": 76, "reference_start": 33, "reference_end": 108, "reference_sequence": "MTPYKKAIDLWKNYYDSYSIGLKEVEEIIWDKLYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLVPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ" }, "paralog": false, "identity": 0.9444444444444444, "score": 213.0, "evalue": 7.19e-73, "description": "Internal stop codon at 390817, 390874. Nonsense mutation around 390817, 390874.", "psc": { "uniref90_id": "UniRef90_UPI0024112F2E", "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024112F2E", "UniRef:UniRef50_UPI0024112F2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9208.525799999992, "isoelectric_point": 7.784702491760254 }, "id": "LMJBGEANGM_701", "locus": "GCA013368015_00688" }, { "type": "cds", "sequence": "contig_1", "start": 391127, "stop": 391546, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACAAAAATTATAACTTCATTAAGTCTATGCTTAACTACTGCTATTTCAACAATAGCAATCTATTCAATAATCGCCGTTAAAAAAAATATAGAACTAGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAGATTGCAATCGCTTATCTCAAATAATCAACACAAAATTCCAAATAGCTATTTAAATTCAATTCAATATTTCCATAATGTAACTAGAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAATGAAATTATTGACGTAAATGAAAAAATTCTTGCCTTTGATGTAGAAATAAAAAATAAACTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTACCATAACAGACTATATAAAATATGA", "aa": "MKKRTKIITSLSLCLTTAISTIAIYSIIAVKKNIELDKSNKYSLFIQEKSRLQSLISNNQHKIPNSYLNSIQYFHNVTRTSKAIEIIDATSKIRNEIIDVNEKILAFDVEIKNKLSLFNEFNQIKQKLENDYHNRLYKI", "aa_hexdigest": "7a823779e8b78ed3d735e94644c1d886", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16221.634499999991, "isoelectric_point": 9.655317115783692 }, "id": "LMJBGEANGM_702", "locus": "GCA013368015_00689" }, { "type": "cds", "sequence": "contig_1", "start": 392039, "stop": 392446, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTTTACTCGCCTTTATTCTTTTATGAACAAACAGAAGAAGTCAAAAAAGTTTTTCAATATCCTGATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAGATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCATTCAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACTAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTTGAAATAAAAAAAGCCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTCGCAATATTCCACAAGAGATAAAAAGAGTATTTGA", "aa": "MFFYSPLFFYEQTEEVKKVFQYPDKKDFEKEIILKYNQAKQDLTNFKSKINGNEKIAKKAFNKFNEYIRNLAISFEKELIQKKLLLLKNLLTKYDQKQDGKSQFITVEIKKAINDAESLLSYNDSQYSTRDKKSI", "aa_hexdigest": "04026a31d53f9187717825d1bee9baf2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16134.348299999985, "isoelectric_point": 9.363146400451662 }, "id": "LMJBGEANGM_703", "locus": "GCA013368015_00690" }, { "type": "cds", "sequence": "contig_1", "start": 392621, "stop": 392854, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961", "UniRef:UniRef90_UPI00215DA961" ], "nt": "TTGAAATTGATATCATCAAATGTTGAGGAATTTAATATTCTTGCAAATGATGTCTATTCTAAAAATCAAAAAATTTCTAACGAAATACAAGAAGTTAGAAAACTTATAGAAGAACACGACGAAAAATTAGAAGATTTTTATGTTAAGAAATCACAGCCAAATTTAAAATATTTAACAGAAATATTACTTAAATTACAAAATAAATTACATTCAATTAGACAAAAAGTCAATTAA", "aa": "MKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHDEKLEDFYVKKSQPNLKYLTEILLKLQNKLHSIRQKVN", "aa_hexdigest": "5aad3073b326245600ae5049dd67e7b9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 392621, "stop": 393061, "observations": { "mutation": [ 392863 ], "stop": [ 392863 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA961", "query_cov": 1.0, "subject_cov": 0.5167785234899329, "identity": 0.909, "score": 132.0, "evalue": 3.25e-38, "gene_start": 1, "gene_end": 77, "reference_start": 71, "reference_end": 147, "reference_sequence": "MLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSKLFSSSDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVKKLIEEHDKKLEDFYIKKLQPNLKYLTEILLKLQNKLYLIRQKVNQI" }, "paralog": false, "identity": 0.9315068493150684, "score": 263.0, "evalue": 5.35e-92, "description": "Internal stop codon at 392863. Nonsense mutation around 392863.", "psc": { "uniref90_id": "UniRef90_UPI00215DA961", "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA961", "UniRef:UniRef50_UPI00215DA961" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9201.473799999994, "isoelectric_point": 7.952707099914551 }, "id": "LMJBGEANGM_704", "locus": "GCA013368015_00691" }, { "type": "cds", "sequence": "contig_1", "start": 392861, "stop": 393451, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B", "UniRef:UniRef90_UPI002410C12B" ], "nt": "ATGTTGAACGAATTATTTAATGATTTCAAGGATATTGATTCAAAAACATTTGCAGAAGTTATTTATCAATTTGAAGAATCAAATAAATTATTGGCCGAATATATTGAATTATATTCACAAATTATTTATAATGCAAAGAATGAATTTGAAATGCAACAAAAAAATAGCGATTTATTTAATTTATTTAGTCAAGAAACAAAAAATAAAATTACAAATTTTTTAGCAACAATTAATAAATTGGAAAATAATAGAAATGATCAACAAATAAAAAAATCTATACATGAAGAATTTAAAAAAATTCTAACTGTCCAAGAAGAAATTTATAAAAATATAAATAAGGATTTTTTAAACATTACTAATAAAGAAGAAAGCATTAAACTTTTAAAAACGCTTAATGAAAGTTTAAAAGAACAAAAAAATTCAGAAGATGGAAATTTTTTAAGTAAATTTCTAAATAATGAAATAGCCAAAATATTAAAAGAAAATTCTGAGCTATTTTCTTCAAAAGATATTGCCAAAATCAATAAAAATATAGAAATGACATACGAACTTTCTAGAAAATTAAACCTGGAAATTATTTTAAATAGGTGA", "aa": "MLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITNFLATINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIKLLKTLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEMTYELSRKLNLEIILNR", "aa_hexdigest": "ba748774c95dbb73fb807f9e814d23a2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 392621, "stop": 393751, "observations": { "mutation": [ 392863, 393472, 393715 ], "stop": [ 392863, 393472, 393715 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410C12B", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.9640000000000001, "score": 343.0, "evalue": 1.17e-115, "gene_start": 1, "gene_end": 196, "reference_start": 141, "reference_end": 336, "reference_sequence": "MKSKEILWNISVQKNLIDQRYTKESLSPKSKIDVEFLKQKRKLENLFSIYKKWNKADMSKEIELLKKINNVNFGFKNDDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKTWDVVDRLMLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITDFLAIINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIRLLKMLNKNLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHEEKLEDFYIKKSQLILKYLTEILLKLQNKLHSIRQKVN" }, "paralog": false, "identity": 0.9547872340425532, "score": 691.0, "evalue": 4.49e-255, "description": "Internal stop codon at 392863, 393472, 393715. Nonsense mutation around 392863, 393472, 393715.", "psc": { "uniref90_id": "UniRef90_UPI002410C12B", "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410C12B", "UniRef:UniRef50_UPI002410C12B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23313.188800000033, "isoelectric_point": 5.347207832336425 }, "id": "LMJBGEANGM_705", "locus": "GCA013368015_00692" }, { "type": "cds", "sequence": "contig_1", "start": 393470, "stop": 393700, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAGGAAATAGAACTTCTTAAAAAGATTAATAATGTTAATTTTAGCTTTAAAAACGAAGATATAGCGAAATTAACTAGTGAAATTTCAAAAAGAATTGAAGATATTTATCAAAAAATAAATTCATCAAAAGATATACAAAAACATTTAAATAATGTTAAGAATGATTTAAATCCACTTATACAAGAATTGATTAACTTTAATAATTTTAAAAATAGCAAAACATGA", "aa": "MSKEIELLKKINNVNFSFKNEDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKT", "aa_hexdigest": "43482a39934705cbb10a0338adb48bdd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8937.110499999995, "isoelectric_point": 9.26070613861084 }, "id": "LMJBGEANGM_706", "locus": "GCA013368015_00693" }, { "type": "cds", "sequence": "contig_1", "start": 393848, "stop": 394303, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGAAAAAACAAACTATTTATTCTATCAACTATCTTAGGATCGTTTGCAACTGTATTTACAGTAATTTCTAGTGCTTCATGTTCTAATAAACAAGATAAAAAAGAAGAAACTAAAGATAGAAAAGAAGATTCAAACAACAACGTAGCAAATACGCCTAATGTTAAACCAAATATATCTAAAGAAAAACAAGAAGATAATAGCCACATAGTTAGTCCTAATATAAAGCCAACTGTTCCAAATGAATCTCAAAAACCAAAAGAAGATAAAAATAATGTAGCAAGTTTTAGTCCTAGTAGGCTACAAGAAGTTAAAAATGAACTGCAAACATATATAGCCAATAATCTTAAAGATTTTATTTATGAAAATTTAAAATTAGAAATTAAAGATTTTATTGAAAAAGATAAAAATGAGGCTGATGAATTTATTTTGAAATCTAAAGAAATTCTTTGA", "aa": "MKRKNKLFILSTILGSFATVFTVISSASCSNKQDKKEETKDRKEDSNNNVANTPNVKPNISKEKQEDNSHIVSPNIKPTVPNESQKPKEDKNNVASFSPSRLQEVKNELQTYIANNLKDFIYENLKLEIKDFIEKDKNEADEFILKSKEIL", "aa_hexdigest": "4672692689605acd6d01f1f1620ab19c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17289.293000000005, "isoelectric_point": 8.512421607971191 }, "id": "LMJBGEANGM_707", "locus": "GCA013368015_00694" }, { "type": "tRNA", "sequence": "contig_1", "start": 394513, "stop": 394596, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 394519, 394521 ], "id": "LMJBGEANGM_20", "locus": "GCA013368015_00695" }, { "type": "tRNA", "sequence": "contig_1", "start": 394600, "stop": 394674, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 65.1, "nt": "TGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 394632, 394634 ], "id": "LMJBGEANGM_21", "locus": "GCA013368015_00696" }, { "type": "cds", "sequence": "contig_1", "start": 394779, "stop": 395000, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145", "UniRef:UniRef90_UPI00093A2145" ], "nt": "TTGAGTAACACAAAAAATGAAACATCTTCTTTTATTAAAAAAAATCTTGAAGGAAAAGCTGAATATAAAGAAGGCTACGATACTTTAAATAAATTAATACAAGAAGCTAAAGAAATTTTGGACAACTCAGAAAAACATAGCAATTTAGAACTAAAAAACAAAGAAGCTGAAATACAAGCCAAATTACTTGAATATAAAAATAAATATAATATGAACAAATAA", "aa": "MSNTKNETSSFIKKNLEGKAEYKEGYDTLNKLIQEAKEILDNSEKHSNLELKNKEAEIQAKLLEYKNKYNMNK", "aa_hexdigest": "225036386580d8650b16a6d2d684f555", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 394779, "stop": 395219, "observations": { "mutation": [ 395009, 395141, 395177 ], "stop": [ 395009, 395141, 395177 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00093A2145", "query_cov": 1.0, "subject_cov": 0.5, "identity": 0.863, "score": 115.0, "evalue": 1.23e-31, "gene_start": 1, "gene_end": 73, "reference_start": 74, "reference_end": 146, "reference_sequence": "MRTDSSNCLDSKKYWEEAEKEISKIRWDALDLEKQGIQEDKLEKFKSDFRALKKPIEDKKNEILDEFADFWTKLSNSKNKTSYFIENKLKGKAEYKEGYDALNKLIQEAKEILNNSEKHSNLELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.910958904109589, "score": 259.0, "evalue": 1.38e-90, "description": "Internal stop codon at 395009, 395141, 395177. Nonsense mutation around 395009, 395141, 395177.", "psc": { "uniref90_id": "UniRef90_UPI00093A2145", "uniref50_id": "UniRef50_UPI00093A2145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00093A2145", "UniRef:UniRef50_UPI00093A2145" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00093A2145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8554.542199999996, "isoelectric_point": 7.876247596740723 }, "id": "LMJBGEANGM_708", "locus": "GCA013368015_00697" }, { "type": "cds", "sequence": "contig_1", "start": 395316, "stop": 399698, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD68537", "UniRef:UniRef90_UPI00158EF5ED", "PFAM:PF10732.14" ], "nt": "TTGCAAAACAACAATGTAGATGCTTCATCAACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAAAGCTAAACAAGACTTTAATTCAAAGAAACAACAACTAGACAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACAAGAAACCGACATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACCAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGACTTTACTGACGCTAAAAAACAATTAGAAGACTTAATTAATTCACAAGACGCACAAAAAGTCGACAAATCTAATGCAAATCAATCATTGCAAAATAACAATGTAGATGCTTCATCGACGACCGATCAAATTGTTAATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTGATTGATGCAGCCAAAGACAAAGCCAAACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAAATCAAATGAAGCTAAAGATGTTGACAAACAAGCAGAAACTGACATCTTTAACAACACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAATTAATGATAAAAAAGACAGTCTTCTTAATGATTTTAATGATGCTAAAAAGAAATTACAAGATTTAATTGATTCACAAGATGGCCAAAAAGTCGACACTTCTAAAGCAAATCAATCATTGCAAAACAACAATGTAGATGCTTCATCAACGACCGATCAAATTGTTGATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAAAAAGCCGCACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACTAAGAAAATTAATGATAAAAAAGACAGTCTTCTTAAAGACTTTAACGACGCTAAAAAGCAATTAAAGGATTTAATTGATTCACAAGATGGCCAAAAAGTCGACACTTCTAAAGCAAATCAATCATTGCAAAATAACAATGTAGATGCTTCATCAACGACCGATCAAATTGTTGATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAACAAGCCACACAAGACTTTAATTCAAAGAAGCAACAATTAGATAATTTGATTAAATCAAATAAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTACTAACAAAGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACCAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGATTTTAATGACGCTAAAAAGCAATTAAAGGATTTAATTGATTCACAAGACGGTCAAAAAGTTGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGCAGATGCTTCATCAACGACCGATCAAATTATTAATGTAACAAATGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAAAAAGCCGCACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACATTGAGTCAAAAACAAAAACCATTGAAGATGCTATTAAATCATTGACCAAGAAAATTAATGATAAAAAAGAAAATCTTCTTAAAGACTTTACTGACGCTAAAAAACAATTAGAAGACTTAATTAATTCACAAGATGGCCAAAAAGTCGATACTTCTAAAGCAAATCAATCATTGCAAAACAACAATGTAAATGCTTCATCAACGACTGATCAAATTATTAATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAAAAAGCTAAACAAGACTTTAATTCAAAGAAGCAACAATTAGAAAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAAGCAGAAACTGATATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGACTTTAACGACGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGACGGTCAAAAAGTTGACACATCTAAAGCAAATCAATCATTGCAAAATAATAATGTAGATGCTTCATCAACGACCGATCAAATTGTTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAAAGCTAAACAAGACTTTAATTCAAAGAAACAACAACTAGACAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACAAGAAACCGACATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACCAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGACTTTACTGACGCTAAAAAACAATTAGAAGACTTAATTAATTCACAAGACGCACAAAAAGTCGACAAATCTAATGCAAATCAATCATTGCAAAATAACAATGTAGATGCTTCATCGACGACCGATCAAATTGTTAATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTGATTGATGCAGCCAAAGACAAAGCCAAACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACTAAGAAAATTAATGATAAAAAAGATAATCTTCTTAAAGACTTTAATGATGCTAAAAAGAAATTACAAGATTTAATTGATTCACAAGACGCACAAAAAGTCGACAAATCTAATGCAAATCAATCATTGCAAAATAACAATGTAGATGCTTCATCGACGACCGATCAAATTGTTAATGCAACAAATGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAAAGCCACACAAGACTTTAATTCAAAGAAGCAACAATTAGATAATTTAATTAAATCAAATGAAGCAAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTACTAACAAAGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGACTTTAACGACGCTAAAAAGCAATTAAAGGATTTAATTGATTCACAAGATGGCCAAAAAGTTGACACTTCTAAAGCAAATCAATCATTGCAAAATAACAACGTAGATGCTTCATCAACAGTCGATCAAATTGCTAATGCAACAAATGAAATCAAAAAAGCCACACAAGATTTGCAAAAATTAATTGACGCAGCCAAAGAACAAGCTAAACAAGACTTTAATTCAAAGAAGCAACAATTAGATAATTTAATTAAATCAAATGAAGCCAATGATGTTGATAAACAAGAAGAAACCAACATCTTTAACAACACAAATCTTACTGGCAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACCAATAAAATTAATGATAAAAAGCCAAAGGAAAACATTGAATATGATGGGCTTGAAAAAATTAGGGAACAAATTAAACAATTTATTGAAAAAGTAAAAGAGGATGAATATTATACAAAATATAGTAGTCAATATTATGATTATGATGATAATATAATAGACCAATTAAACAAACAACTTAAGTTCTTTGAAAACGTTACTTCTGCTTCGGACATTCAACAAATATCTGGGGCTAAGTGA", "aa": "MQNNNVDASSTTDQIVNATNEIKKATQDLQKLIDAAKEKAKQDFNSKKQQLDNLIKSNEAKDVDKQQETDIFNNTNLSGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFTDAKKQLEDLINSQDAQKVDKSNANQSLQNNNVDASSTTDQIVNATTEIKKATQDLQKLIDAAKDKAKQDFNSKKQQLDDLIKSNEAKDVDKQAETDIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKINDKKDSLLNDFNDAKKKLQDLIDSQDGQKVDTSKANQSLQNNNVDASSTTDQIVDATTEIKKATQDLQKLIDAAKEKAAQDFNSKKQQLDDLIKSNEAKDVDKQPETDIFNNTNLSGNDLIKDIESKTKTIEDAIKSLTKKINDKKDSLLKDFNDAKKQLKDLIDSQDGQKVDTSKANQSLQNNNVDASSTTDQIVDATTEIKKATQDLQKLIDAAKEQATQDFNSKKQQLDNLIKSNKAKDVDKQPETDIFNNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKDNLLKDFNDAKKQLKDLIDSQDGQKVDTSKANQSLQNNNADASSTTDQIINVTNEIKKATQDLQKLIDAAKEKAAQDFNSKKQQLDDLIKSNEAKDVDKQPETDIFNNTNLSGNDLIKDIESKTKTIEDAIKSLTKKINDKKENLLKDFTDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVNASSTTDQIINATTEIKKATQDLQKLIDAAKEKAKQDFNSKKQQLENLIKSNEAKDVDKQAETDIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDASSTTDQIVNATNEIKKATQDLQKLIDAAKEKAKQDFNSKKQQLDNLIKSNEAKDVDKQQETDIFNNTNLSGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFTDAKKQLEDLINSQDAQKVDKSNANQSLQNNNVDASSTTDQIVNATTEIKKATQDLQKLIDAAKDKAKQDFNSKKQQLDDLIKSNEAKDVDKQPETDIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKINDKKDNLLKDFNDAKKKLQDLIDSQDAQKVDKSNANQSLQNNNVDASSTTDQIVNATNEIKKATQDLQKLIDAAKEKATQDFNSKKQQLDNLIKSNEAKDVDKQPETDIFNNTNLTNKDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLKDLIDSQDGQKVDTSKANQSLQNNNVDASSTVDQIANATNEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSNEANDVDKQEETNIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTNKINDKKPKENIEYDGLEKIREQIKQFIEKVKEDEYYTKYSSQYYDYDDNIIDQLNKQLKFFENVTSASDIQQISGAK", "aa_hexdigest": "d29b63d0faf1c255c7a28a71b9253b7b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00158EF5ED", "query_cov": 1.0, "subject_cov": 0.8907870652837095, "identity": 1.0, "score": 2086.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_UPI000CD68537" }, "pscc": { "uniref50_id": "UniRef50_UPI000CD68537", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CD68537" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF10732.14", "name": "DUF2524", "length": 46, "aa_cov": 0.031506849315068496, "hmm_cov": 1.0, "evalue": 2.5231894983247994e-07, "score": 26.481122970581055, "start": 796, "stop": 841 } ], "seq_stats": { "molecular_weight": 163992.11690000092, "isoelectric_point": 5.027147483825684 }, "id": "LMJBGEANGM_709", "locus": "GCA013368015_00698" }, { "type": "cds", "sequence": "contig_1", "start": 399758, "stop": 400132, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936738.1", "SO:0001217", "UniParc:UPI00158958F3", "UniRef:UniRef100_UPI00158958F3", "UniRef:UniRef50_UPI0005C8D5F1", "UniRef:UniRef90_UPI00158958F3" ], "nt": "TTGCAAAACAACAATGTAGATGCTTCATCAACGACCGATCAAATTATTAATGCAACAAATGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAAAAAGCTAAACAAGACTTTAATTCAAAGAAGCAACAATTAGAAAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAAGCAGAAACTGACATCTTTAACAACACAAATCTTTCTGGTAATGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAGAACCAAAAGACAATCTTCTTAAAGACTTTAACGACGCTAAAAAACAATTAG", "aa": "MQNNNVDASSTTDQIINATNEIKKATQDLQKLIDAAKEKAKQDFNSKKQQLENLIKSNEAKDVDKQAETDIFNNTNLSGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKTIFLKTLTTLKNN", "aa_hexdigest": "d405de4fc1ad2835b78dbc3360fd5980", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158958F3", "ncbi_nrp_id": "WP_175936738.1", "uniref100_id": "UniRef100_UPI00158958F3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158958F3", "RefSeq:WP_175936738.1", "UniRef:UniRef100_UPI00158958F3" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158958F3", "uniref90_id": "UniRef90_UPI00158958F3" }, "psc": { "uniref90_id": "UniRef90_UPI00158958F3", "uniref50_id": "UniRef50_UPI0005C8D5F1" }, "pscc": { "uniref50_id": "UniRef50_UPI0005C8D5F1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8D5F1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13983.489599999974, "isoelectric_point": 7.818548393249512 }, "id": "LMJBGEANGM_710", "locus": "GCA013368015_00699" }, { "type": "cds", "sequence": "contig_1", "start": 400191, "stop": 401429, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936739.1", "SO:0001217", "UniParc:UPI00158E2439", "UniRef:UniRef100_UPI00158E2439", "UniRef:UniRef50_UPI00193AD2FA", "UniRef:UniRef90_UPI00158E2439" ], "nt": "ATGAAAAAATCGAAATTAAGAATTTTACTTTCTACTTCGTTGATTATTGCTCTAGCAACAACGGCCACCTTAGTTGCAACATCGCTAAAAAGCAAATTTAAAAGAAGACAATATGAACAAGAAGATAATATTAACAAATTAAAGGAAAAATTCAATAGATTAAAATCAAACCTAGACAATTTGATTAAATCAAATGAAGCTAAAGATGTTGACAAACAAGCAGAAACTGACATCTTTAACAACACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAATTAATGATAAAAAAGACAGTCTTCTTAATGATTTTAATGATGCTAAAAAGAAATTACAAGATTTAATTGATTCACAAGATGGCCAAAAAGTCGACACTTCTAAAGCAAATCAATCATTGCAAAACAACAATGTAGATGCTTCATCAACGACCGATCAAATTGTTGATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAAAAAGCCGCACAAGACTTTAATTCAAAGAAGCAACAATTAGATGATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTTCTAACAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACTAAGAAAATTAATGATAAAAAAGACAGTCTTCTTAAAGACTTTAACGACGCTAAAAAGCAATTAAAGGATTTAATTGATTCACAAGATGGCCAAAAAGTTGACACTTCTAAAGCAAATCAATCATTGCAAAACAACAATGTAGATGCTTCATCAACGACCGATCAAATTATTGATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTACAAAAATTAATTGATGCAGCCAAAGAACAAGCCACACAAGACTTTAATTCAAAGAAGCAACAATTAGATAATTTAATTAAATCAAATAAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAACACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTGACCAAGAAAATTAATGATAAAAAGACAATCTTCTTAAAGACTTTACTGACGCTAAAAAGCAATTAG", "aa": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNINKLKEKFNRLKSNLDNLIKSNEAKDVDKQAETDIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKINDKKDSLLNDFNDAKKKLQDLIDSQDGQKVDTSKANQSLQNNNVDASSTTDQIVDATTEIKKATQDLQKLIDAAKEKAAQDFNSKKQQLDDLIKSNEAKDVDKQPETDIFNNTNLSNNDLIKDIESKTKTIEDAIKSLTKKINDKKDSLLKDFNDAKKQLKDLIDSQDGQKVDTSKANQSLQNNNVDASSTTDQIIDATTEIKKATQDLQKLIDAAKEQATQDFNSKKQQLDNLIKSNKAKDVDKQPETDIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKINDKKTIFLKTLLTLKSN", "aa_hexdigest": "e9565c8b8338f1808ff42171dde6974a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158E2439", "ncbi_nrp_id": "WP_175936739.1", "uniref100_id": "UniRef100_UPI00158E2439", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E2439", "RefSeq:WP_175936739.1", "UniRef:UniRef100_UPI00158E2439" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E2439", "uniref90_id": "UniRef90_UPI00158E2439" }, "psc": { "uniref90_id": "UniRef90_UPI00158E2439", "uniref50_id": "UniRef50_UPI00193AD2FA" }, "pscc": { "uniref50_id": "UniRef50_UPI00193AD2FA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD2FA" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 46286.41760000003, "isoelectric_point": 8.64799861907959 }, "id": "LMJBGEANGM_711", "locus": "GCA013368015_00700" }, { "type": "cds", "sequence": "contig_1", "start": 401683, "stop": 402732, "strand": "-", "frame": 3, "gene": null, "product": "MSC_0618 family F1-like ATPase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0004864FE0" ], "nt": "ATGAATTCAACTATAAACAATGAAAGATATCTAGAAAATAGCAAAGAAGTTATAGAAACCGGAATAAAAGCAATTGACTTCTTTATGCCAATTGTTAAGGGCGCAAAAATCGGTTTATTTGGAGGAGCCGGAGTTGGAAAAACTGTTCTAATGAAGGAAATTATTTTTAACCTTAATAAACAACATGAAGCAAATTCTAATATCTTTATTGGTTCGGGTGAACGTTCAAGAGAAGGAATAGAATTATACAATGAACTTTCTTCATCTAATTTAATGAAGGACTCAATAATGTTTATTTCGAAAATGAATGAAGCACCAGGAGCTAGAACGTCAATTGTTCCAATCGGAATTACTGCTGCAGAATATTTAAGAGACACATGCAACGAAAACGTTTTGTTATTTATTGACAATATTTATCGTTTTATTCAAGCAGAAAATGAAGTAAGTTCAGCATTAGGCAAAAAGCCTTCAATCGGAGGTTACCAATCAACTTTAGAAAGTGATGTAACCGAAGTTGAAAGCAGATTGTTTAGAAACAAGAAGGGCTCAATAACTTCATTCCAAACAATATTTCTACCAATGGATGATTTAAGCGACCCTTCTGCAGTAGCTGTATTTAATCACTTAGATGGTAATTTGGTCTTATCTAGATCTCAAGCAGCTAAAAACATTTTTCCTGCCTTTGACCCACTTGCTTCATCTACAAACGCAATTAATCCAACCATTATTGGCAAACGTCACTATGATGCAATAATTGAAGCAAAAAGAATATTAAAGGCATATAAAGATCTTGAAGATGTTATTCTGATATTGGGATTTGATGAACTTGATGAAGAAAGCAAAATCACTGTTAAAAAAGCTCTTCAACTTGAAATGTTTTTTACTCAAAATTTCTTTATGACTGAACACTTTACTAAATCTCCTGGTCAATTTGTGCCATTAAACGAAACAGTTGAAAGCGTTATAAGAATATTGGAAGGAAGATACTTAAAACAAAATCCTGAAATTTTTGCTTATATAGGTTCAAATAAGGATATTCAGCAAGATTAA", "aa": "MNSTINNERYLENSKEVIETGIKAIDFFMPIVKGAKIGLFGGAGVGKTVLMKEIIFNLNKQHEANSNIFIGSGERSREGIELYNELSSSNLMKDSIMFISKMNEAPGARTSIVPIGITAAEYLRDTCNENVLLFIDNIYRFIQAENEVSSALGKKPSIGGYQSTLESDVTEVESRLFRNKKGSITSFQTIFLPMDDLSDPSAVAVFNHLDGNLVLSRSQAAKNIFPAFDPLASSTNAINPTIIGKRHYDAIIEAKRILKAYKDLEDVILILGFDELDEESKITVKKALQLEMFFTQNFFMTEHFTKSPGQFVPLNETVESVIRILEGRYLKQNPEIFAYIGSNKDIQQD", "aa_hexdigest": "0cdd352669eeb3cb0fd7e3fc6262d036", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0004864FE0", "query_cov": 0.8997134670487106, "subject_cov": 0.9843260188087775, "identity": 0.7609999999999999, "score": 485.0, "evalue": 2.05e-170, "valid": false, "product": "MSC_0618 family F1-like ATPase beta subunit", "uniref50_id": "UniRef50_UPI0004864FE0" }, "pscc": { "uniref50_id": "UniRef50_UPI0004864FE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0004864FE0" ], "product": "MSC_0618 family F1-like ATPase beta subunit" }, "genes": [], "id": "LMJBGEANGM_712", "locus": "GCA013368015_00701" }, { "type": "cds", "sequence": "contig_1", "start": 402778, "stop": 402963, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGACATTGCATGAAGGAAAAACCTTTTTACTAGTAAAAAGATTAATTGACGAAAAACATGTTAGAGCAATTGTTGTATATGCAAGTAAAGATATTTCAATTAACGAAGAAGTTGTTAACACTCATAAGAGTTTTTTAGTTCCAATAGGGCAAAATTCAAAAAACAATATTTACAACTTTTGA", "aa": "MLTLHEGKTFLLVKRLIDEKHVRAIVVYASKDISINEEVVNTHKSFLVPIGQNSKNNIYNF", "aa_hexdigest": "b79f7e5270817dd9609d107154fc9955", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7026.0805, "isoelectric_point": 9.157943534851075 }, "id": "LMJBGEANGM_713", "locus": "GCA013368015_00702" }, { "type": "cds", "sequence": "contig_1", "start": 403122, "stop": 403304, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGAATAAAAGATGAACAAAAAGCATTCATGCTACTAGATTATTTAATAAATAATTATAATGATTGCATAAAAGCATTTAAAACAATTTTAGATAATTTAGACTACGATGATAATATTATGAAGAATTATTTTGCATTTGTCTTGAAATCATATTCAGATTATTTAAATTTAAATTGA", "aa": "MQGIKDEQKAFMLLDYLINNYNDCIKAFKTILDNLDYDDNIMKNYFAFVLKSYSDYLNLN", "aa_hexdigest": "c0f4e2da180052d69cbdc178b387d2c4", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7186.1301, "isoelectric_point": 4.425506782531738 }, "id": "LMJBGEANGM_714", "locus": "GCA013368015_00703" }, { "type": "cds", "sequence": "contig_1", "start": 403311, "stop": 404255, "strand": "-", "frame": 1, "gene": null, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "nt": "ATGTTGAGCAATCATCATCCAATATTTGGCCTAGCTCATAATTTGATGACAGTTAAAACATTAAATGAACAACTTTATACTGGAATTATTGCAATAGATTTATTAATTCCAATTGGAAAAGGTCAACGTGAACTAATTATTGGCGATAGGCAAACTGGAAAAACTCATATGGCATTGAATGCAATTATAAATCAAGCAAAAAATGGTACAAAATGTATCTATGTTGCAATTGGCCAAAAGCGTGAATCATTAACTAATATTTATGAAACATTAAAAAAATACGATGCATTGAAAAACACTATGATTATTGATGCACCTCAAAACGTTTATGAACAATATCTAGCGCCTTACATTGGAATGGCCCATGCAGAAAATCTTTCATATGACAATGATGTTTTAATAGTATTTGATGATTTAACTAAACACGCTAATATTATTAGAGAAATTGCCCTATTAACTAACAAGTCAGTTGGAAAAGAAGCAATGCCTAGCGACATCTTTTTTGCCCATTCATCATTGCTTGAAAGAGCTGGATCTTTTGTCAATAGAAAAACAATTACTGCACTTCCAATTTTACAAACAGTTGACGGCGATATAACTAGTTTAATTTCATCTAATATAATTTCAATAACTGATGGTCAAATCGTAACTTCTTCTGACTTATTTGCTTCAGGGAAATTACCTGCTATAAACATTGATTTATCTGTATCAAGAACAGGTTCAAGTGTTCAATCAAGAATGATGACAAAAATAGCTGGTGAAATTAATAAAATTTTTAGAAGTTACAAGAGACATTTAAAATTAGCAATGTTAGATTACGAGCTTAATAAAGAAACTTCTATTTTGCTTCACAAAGGAAAATTAATTGACAAAATGTTCTTGCAAAAGGGTTTTTCACTTTACTCATATCGTTTTGTAGTGCTAACAACAAAATTAATTTCTTGA", "aa": "MLSNHHPIFGLAHNLMTVKTLNEQLYTGIIAIDLLIPIGKGQRELIIGDRQTGKTHMALNAIINQAKNGTKCIYVAIGQKRESLTNIYETLKKYDALKNTMIIDAPQNVYEQYLAPYIGMAHAENLSYDNDVLIVFDDLTKHANIIREIALLTNKSVGKEAMPSDIFFAHSSLLERAGSFVNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSSDLFASGKLPAINIDLSVSRTGSSVQSRMMTKIAGEINKIFRSYKRHLKLAMLDYELNKETSILLHKGKLIDKMFLQKGFSLYSYRFVVLTTKLIS", "aa_hexdigest": "191f1297b78296469ae700fd94d6d26a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FAQ0", "query_cov": 0.8853503184713376, "subject_cov": 0.8938906752411575, "identity": 0.525, "score": 251.0, "evalue": 1.05e-78, "valid": false, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "uniref50_id": "UniRef50_A0A432FAQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A432FAQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_715", "locus": "GCA013368015_00704" }, { "type": "cds", "sequence": "contig_1", "start": 404289, "stop": 404579, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACACAACTAAAAATCCTCGTATTAAATCAATATTCAATTGCATAGCAACAATTGAGGGTGAATACAACTACCGTCAAAATCAACAATTTTATTTAGCAAGTAACCCAAATGTTAAATTCATAGTTATTTCTGCTACAACTAATAAGGCATTTATGTTGATTAATAGTGAACACGTATCTTTAGATATAAATAGTGAATTAATTGAATTAAATGAATCCCAAAATATAAATACTAGTCTTGATTATTTGGGCAAAATTATTGATATTAATGGTGGCGTCATTTGA", "aa": "MNNTTKNPRIKSIFNCIATIEGEYNYRQNQQFYLASNPNVKFIVISATTNKAFMLINSEHVSLDINSELIELNESQNINTSLDYLGKIIDINGGVI", "aa_hexdigest": "689894a023341db3c52f8aa6e8fc1b8f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10842.134999999991, "isoelectric_point": 5.187547111511229 }, "id": "LMJBGEANGM_716", "locus": "GCA013368015_00705" }, { "type": "cds", "sequence": "contig_1", "start": 404595, "stop": 404924, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTGGTATAAAACATAATTTTGACTTATTATCAAATTTAGCAAGTAATATTTCAATAAAAGCTAATAAAGAAGAAAAAGAAAATTTCAATAAAGCTTATTTAGAAGCAGTCAATGTTTTAAATAAAAATTATCTTGAACAAAAGAAGATTATTAAAAAACTAGGGACTTCTTTTATAGTTATTTCTTTAATCATAATAATTACAAACATAATAATTTTTGCATTGTTAAAAAACTTAAAATTAAAGAAAAAGGTAATTATTATCAATAGTGTAATACTAGCGATCGCATTACTAATATTAATAATTGGAGGGCTATTATTTATATAG", "aa": "MSGIKHNFDLLSNLASNISIKANKEEKENFNKAYLEAVNVLNKNYLEQKKIIKKLGTSFIVISLIIIITNIIIFALLKNLKLKKKVIIINSVILAIALLILIIGGLLFI", "aa_hexdigest": "40d5d3327595f25768d062261b24a46e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12201.784299999981, "isoelectric_point": 9.948970603942868 }, "id": "LMJBGEANGM_717", "locus": "GCA013368015_00706" }, { "type": "cds", "sequence": "contig_1", "start": 404946, "stop": 405113, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAATTTAGTGAAGCGCTTAAATTCAATGAAAAATTCATTAAAACAAATTTAGCAGAAATCAAAGGCAATGAGAATGTTTTAAGAGAATTATTCTTATTAAATAAACAATTAAACTACAAATTTACCGCTTTAATTAGCCAAAGAGATTTCAACCAAGAAAAATGA", "aa": "MQFSEALKFNEKFIKTNLAEIKGNENVLRELFLLNKQLNYKFTALISQRDFNQEK", "aa_hexdigest": "5bc6f80562418da7b41a1dde956cf919", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6596.5232, "isoelectric_point": 9.156976509094239 }, "id": "LMJBGEANGM_718", "locus": "GCA013368015_00707" }, { "type": "cds", "sequence": "contig_1", "start": 405267, "stop": 405842, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGATTTAAAGCCATTGGTTGATGGTGATAAAACAAGCCACTTAACAAAAGAACAAGAAAAAAATTTAATTCAATCATTGCCCCTTTTGGTTCCGTTTATATCAAGCAACAATTTGCCTTCTTCATTAAGTCAAATTAAAAGCCAATTTGATTCATTAGATGCAAGCAAACGTCAAGAATTATTTTATTTCAATAACCCAATAAATACTCGTTATAAATATTCTGTTTTTTCATTTGATATTAGTGGCAATGATATTATTAATTTAAAAGTACATATTTCAGATAATATAAATCCTAAGTTAGAAAGAACATATTTAATTGAAAAATATACTCCGTCACAAGACAAATATTTTAATCTGTTAAAAGAAACTGAAATAAAAGCAATTTCAGAAATATTTAAGCCATTATATAAAGGTTTGGGCTTAGATGAAAAGTTAGATTACAATAAGCTAAGAAATGGATATTTAAGAAAAGCATTGCTTGCAATGATTGACTCGGCGTTACAAATGTCATTTAAGGCTGATAAAGATTCATATAGTGCAATTAGCAATAAAATATTGCAAAAATATTGA", "aa": "MPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFSFDISGNDIINLKVHISDNINPKLERTYLIEKYTPSQDKYFNLLKETEIKAISEIFKPLYKGLGLDEKLDYNKLRNGYLRKALLAMIDSALQMSFKADKDSYSAISNKILQKY", "aa_hexdigest": "7659a8700b9556a314f37bd6a94ce1da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22043.018500000002, "isoelectric_point": 9.001156425476076 }, "id": "LMJBGEANGM_719", "locus": "GCA013368015_00708" }, { "type": "cds", "sequence": "contig_1", "start": 405951, "stop": 406652, "strand": "-", "frame": 1, "gene": null, "product": "MSC_0620 family F1-like ATPase-associated subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37", "UniRef:UniRef90_UPI0034D97E37" ], "nt": "ATGAATAAAAAGAAAATCTTATTATTATCATCATTTGCAACAGTTCCTTTATTATCTCCAATAGTAGTTTCGGCAGCGCCAAACAATGATCCAAATAATAAAAAACCTGAGTTAGATCCAAATTTTGGTGAATTTGAAAGTGAAGCTAAAAAAACAATTGCTTCAACAATAGAAGATGGTTTAAAAGCAATAATCAATTATTTGCAAGTTCAACAACAAAGGTTGCTAGAAAATAAAGAATTAGAATTTAAATTAAAAATTCAACAATTAATTTATTTAAAAAATTTAGAAACATTTCTAACAAAAAATAAAGAAGAGATTCAAAAAAATCCTAATAAGTATGGAATTTATTTAAATACTCCCCTAATATTAGGAAAAGAAAAAAACCATGATTTGCAAGATATTGATTACGAAGGCGATTTATTTAAATCAATAAAAACTGGTAAATCCGATCCATTAGATTATAAAAAAGCAATTTTGCCAAAAGGAAGTGTTAAAGAAGTTGCTAAAGATCAATTAAATACTGTTAAAAAAGATAAGTATTCTCAATTTTTAAAGAAATATAAATCTGATTTCTTAAAAGAAGTCAATAAGTTATTTTTTAATGAAAAAGATGTTTTAAATATTGACAAAGATGTTGAACTAATTAGGGATAAAAATGGAGTATTTTCTACCACCTTGCCAAAAGGCTTTAAAAGTTGA", "aa": "MNKKKILLLSSFATVPLLSPIVVSAAPNNDPNNKKPELDPNFGEFESEAKKTIASTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKNPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKSDPLDYKKAILPKGSVKEVAKDQLNTVKKDKYSQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKS", "aa_hexdigest": "1b473c0242a40d1a1859e49a424d0ab6", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 405648, "stop": 406652, "observations": { "insertion": [ 406049 ], "deletion": [ 406121 ], "mutation": [ 405952 ], "stop": [ 405952, 406061, 406082, 406088, 406115 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D97E37", "query_cov": 1.0, "subject_cov": 0.6638176638176638, "identity": 0.863, "score": 367.0, "evalue": 3.3e-125, "gene_start": 1, "gene_end": 233, "reference_start": 1, "reference_end": 232, "reference_sequence": "MNKKKILLLSFATVPLLSPIVVSAASNNDPNNKKPELDPNFGEFESEAKKTISSTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKDPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKTDPLDYKKAILPKGSVKEVAKDQLNAVKKDKYYQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKSWDEYFISKFKVRITKFDLKQNQETNEAEQKEEKNPDSLPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFFIWY" }, "paralog": false, "identity": 0.9191616766467066, "score": 578.0, "evalue": 4.92e-211, "description": "Internal stop codon at 405952, 406061, 406082, 406088, 406115. Frameshift due to insertion around 406049. Frameshift due to deletion around 406121. Nonsense mutation around 405952.", "psc": { "uniref90_id": "UniRef90_UPI0034D97E37", "product": "MSC_0620 family F1-like ATPase-associated subunit", "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D97E37", "UniRef:UniRef50_UPI0034D97E37" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37" ], "product": "MSC_0620 family F1-like ATPase-associated subunit" } }, "id": "LMJBGEANGM_720", "locus": "GCA013368015_00709" }, { "type": "cds", "sequence": "contig_1", "start": 407011, "stop": 407112, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTATATAAAATTGAATTACTTTTTTTAGAAAATAAGAAGCTAAATATTGAAAAAGGCTTAGTTTATATTAATGTCAATGAAGAAAATACATGA", "aa": "MNNLYKIELLFLENKKLNIEKGLVYINVNEENT", "aa_hexdigest": "bf89cffbd4c768243ac5d4ea37c19f42", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3954.544500000001, "isoelectric_point": 5.062103462219238 }, "id": "LMJBGEANGM_721", "locus": "GCA013368015_00710" }, { "type": "cds", "sequence": "contig_1", "start": 407105, "stop": 407593, "strand": "-", "frame": 2, "gene": null, "product": "MSC_0622 family F1-like ATPase gamma subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95", "UniRef:UniRef90_D1J8A4" ], "nt": "TTGCAATCTTATGGAAATTCAAATATTGAAAATTTGGCAGAAAAATTAACCAAAATAGTAATGATCTTGTATGAATATGAAAATTACGAAGAAGTCAATTTCGTAGTTAACTCCAATAAAAATTTTAATCAATATTTTACAATTTTGCCAATGTCTAAATTCTCGTTTGAAAAATTTCCTTTAAATGAAAGCAATAATAATTTATATGACATAGATTTTAAAAAAGCAAAAATATATCCTAATATTAATGATTTTATTAATTCTCAAATTAATTGTTATTTATTAAACACTATAAATATGTTAATATCTGAATCTGCATTTTATAAAGCAAAAATAGGTTTAGTTTCAACTAATACAATTTTAAAAGATTTAGACGAAAGTATTCTTAAACTTAAAAAGCAAATATCAAGAGCAAAAAGTGAATTACAAATTGAGGAACTAAATTTATTGACTAAAAAAGACCCTGATGAAGGTGATATTTATGAATAA", "aa": "MQSYGNSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE", "aa_hexdigest": "bfdf87bc05b2ecd0ec114d8fcd1cfcba", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 407105, "stop": 407893, "observations": { "mutation": [ 407746 ], "stop": [ 407746 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8A4", "query_cov": 1.0, "subject_cov": 0.5436241610738255, "identity": 0.988, "score": 308.0, "evalue": 8.19e-104, "gene_start": 1, "gene_end": 162, "reference_start": 137, "reference_end": 298, "reference_sequence": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESRHRFNLFRKINRNILWIYLTEEQKYSTDSYSRYEKTILSNIKKSNNEFILIGKNAIKFGQDNNLQVLQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE" }, "paralog": false, "identity": 0.9885496183206107, "score": 508.0, "evalue": 4.63e-185, "description": "Internal stop codon at 407746. Nonsense mutation around 407746.", "psc": { "uniref90_id": "UniRef90_D1J8A4", "product": "MSC_0622 family F1-like ATPase gamma subunit", "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8A4", "UniRef:UniRef50_Q9PR95" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95" ], "product": "Uncharacterized protein UU050" } }, "id": "LMJBGEANGM_722", "locus": "GCA013368015_00711" }, { "type": "cds", "sequence": "contig_1", "start": 407744, "stop": 408001, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTAAAAGATTTAGAGAATAAACGCAAAAGTTTAAACAATATTTCATTAAAAGTAAATAATGAAAAAAATATTTATTTAATAAATATTATGAAACTTAACCAAAGATTAAGCTTTTTTAATAAAAATGCATTGGTATCAAGGCAAATAATTTTGCATTTAAAAGAAATCTACAATATTAAGAATGATATGTTAAGGAGAGAAAGTCATCATAGGTTTAATTTATTTAGAAAAATAAATAGAAACATATTGTGA", "aa": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESHHRFNLFRKINRNIL", "aa_hexdigest": "8f4cc15ed9b3f3fcb9f21307f0de9739", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10419.179999999986, "isoelectric_point": 10.83953685760498 }, "id": "LMJBGEANGM_723", "locus": "GCA013368015_00712" }, { "type": "cds", "sequence": "contig_1", "start": 408003, "stop": 408506, "strand": "-", "frame": 1, "gene": null, "product": "DUF2714 domain-containing protein", "db_xrefs": [ "RefSeq:WP_175936742.1", "SO:0001217", "UniParc:UPI00158BD6D5", "UniRef:UniRef100_UPI00158BD6D5", "UniRef:UniRef50_D1J8A5", "UniRef:UniRef90_D1J8A5" ], "nt": "ATGAAAAAGAAAGACAATAATCCATTATTGAGTTATTTTGATGTATTTAAAGAATATAGAGAACACGAGTCTTCACAAGATTTAATTACTTTTGATGAAATGATATCAACAGTATTGTTTAATAACAACCTAGGATTTAATTCTCCTATTTATTTAGATTTTATAAGCAAAATTGATTTGGCTTTTAAAAATAAATATGACATTTTACTAAAGGATTTTAACATTACTTTTAATTTGAATTTGAAATTCTCACAAGAGGTATTAGTACCTATAATTACAACAAAAAATTATTCTGCCAATAACGCAATTGATTTAACCACTTCTAGCGATATAAAATACCAAGAATTTTTGAATTCATATAATGCTGAAATAAAAAAACTTTTGGCCCTTAAAAAATCAATTAAGATATTTCCACATGTAATTTTATTTCATTCAGAATTAAAAAATGATTTAGTTTTATTATTTAGTGAAAAAGTAGTAGCAGTTGTTACAAAGGAACAATAA", "aa": "MKKKDNNPLLSYFDVFKEYREHESSQDLITFDEMISTVLFNNNLGFNSPIYLDFISKIDLAFKNKYDILLKDFNITFNLNLKFSQEVLVPIITTKNYSANNAIDLTTSSDIKYQEFLNSYNAEIKKLLALKKSIKIFPHVILFHSELKNDLVLLFSEKVVAVVTKEQ", "aa_hexdigest": "3fce49b9fc36751e1c6aec81ec047f0e", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158BD6D5", "ncbi_nrp_id": "WP_175936742.1", "uniref100_id": "UniRef100_UPI00158BD6D5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158BD6D5", "RefSeq:WP_175936742.1", "UniRef:UniRef100_UPI00158BD6D5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158BD6D5", "uniref90_id": "UniRef90_D1J8A5" }, "psc": { "uniref90_id": "UniRef90_D1J8A5", "product": "DUF2714 domain-containing protein", "uniref50_id": "UniRef50_D1J8A5" }, "pscc": { "uniref50_id": "UniRef50_D1J8A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8A5" ], "product": "DUF2714 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_724", "locus": "GCA013368015_00713" }, { "type": "cds", "sequence": "contig_1", "start": 408487, "stop": 408918, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAGCAATTGTTCAAATAGTGCTTGCGTTAAGTTTGCTATTGACTACATTATTTAACACCAATAGCATTGTTTCTTTAATCAACGTTGCTGTAACGGCAACAACACAAATAGTAATATTATTTGTGTTTGTTAAACAAAACAAGAATATTTCATTGCTACTATTAATAATAATTAAACTAGCAATAATCTCTTCAATTTTAATCTTTGCAATTATTGGATTTAATTATATTTTAACTGCTGATGGCAATGAAAATTATTTATTTTCAATAATTAAAAGTAGAATGAATATTATTCAAATTATTTTACTTTTAAACTTTAGCATTGCATTTATTATGGCATTATCTTTATTGCTTAAATTTATTTTGGCAATTTATTATATAAGAAAATTAAATAAGGAGAATGAATATGAAAAAGAAAGACAATAA", "aa": "MNLAIVQIVLALSLLLTTLFNTNSIVSLINVAVTATTQIVILFVFVKQNKNISLLLLIIIKLAIISSILIFAIIGFNYILTADGNENYLFSIIKSRMNIIQIILLLNFSIAFIMALSLLLKFILAIYYIRKLNKENEYEKERQ", "aa_hexdigest": "a70019bda0113a40c59d0350050772ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16237.546499999988, "isoelectric_point": 9.646098136901855 }, "id": "LMJBGEANGM_725", "locus": "GCA013368015_00714" }, { "type": "cds", "sequence": "contig_1", "start": 409090, "stop": 409314, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTAGGCAATAAATTTTATGAAACAATTAGAGATATTTTAACAATTAAATCTGCTAGCAATTTTGTGATTATTGTATTGACATTTTTAGTTTTAATTACTTTTATAGTATTATCTAATATTAAATTCTTTGCTTTATTGATAAATAAAAAATATGATAAGTTCTATTTAAAAAATCAATTACTATTTTTATTAGTATTATTTGGGGCTGTATTTATTTGA", "aa": "MFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKKYDKFYLKNQLLFLLVLFGAVFI", "aa_hexdigest": "743c1e88d16c38a31320c8d39d0fcea8", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8658.491099999996, "isoelectric_point": 9.822999382019042 }, "id": "LMJBGEANGM_726", "locus": "GCA013368015_00715" }, { "type": "cds", "sequence": "contig_1", "start": 409342, "stop": 409593, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTTATTGCTTTCATTAACTTCTTATACATTATTTTTTAGCTATTTTAAAATTCTTCCAAGCAAATTATTTACAATATCATTTATTTTGATTGCTTTATATATTATTAATTTTAGCTATGAAATATTTAACTTCTTAATTCAAAGAAAAACAAATCCATTGCTTTATAAGAATAAAAATATTTTAATAATTTCTTTAACATTTCAAGGAATCTTAACAATATTATTTGTTGCAATATCATTTGTTTGA", "aa": "MYLLLSLTSYTLFFSYFKILPSKLFTISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFV", "aa_hexdigest": "39984c728aeb78fc765725c621b55d3c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9789.76419999999, "isoelectric_point": 9.756274604797362 }, "id": "LMJBGEANGM_727", "locus": "GCA013368015_00716" }, { "type": "cds", "sequence": "contig_1", "start": 409633, "stop": 409968, "strand": "-", "frame": 3, "gene": null, "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527", "UniRef:UniRef90_UPI001E41E527" ], "nt": "ATGAAACAAAAACAAATCGATTCGAATAATATAAATCAAAAGTTGATTTATGATGATGAATATTCAATAAAAAATCAGCAGAAAAACAACATTTTAAATAAAGTGTTGAAATACCTTTTGATTGTAATTAGTCTTGTATTTATTACAACTTTATTAATTTTTAGCGAAAAGACACTTTTTGCTAATAAAATGTTGCTTTCTAATTCACTAAATTCATTTTTTGACTTTTCAAGTCCAAGAAATCAACAATTAAACTTTTTAATAATTTATAGATTCTTAATTTTATCCTTTGTTTTATTTTATAGTTTAGTAAAAGGCTTTGCTAATTTATATTGA", "aa": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNILNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFLILSFVLFYSLVKGFANLY", "aa_hexdigest": "9dff37942777a8458287bbf36725c0ab", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 409330, "stop": 409968, "observations": { "mutation": [ 409344, 409635 ], "stop": [ 409344, 409635 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E41E527", "query_cov": 1.0, "subject_cov": 0.4036363636363636, "identity": 0.991, "score": 197.0, "evalue": 2.58e-61, "gene_start": 1, "gene_end": 111, "reference_start": 1, "reference_end": 111, "reference_sequence": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNILNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFFILSFVLFYSLVKGFANLYWHKVTIKKYIPFYFLYLLLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFVWINLGINKDILFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKNMINSI" }, "paralog": false, "identity": 0.9811320754716981, "score": 399.0, "evalue": 3.29e-143, "description": "Internal stop codon at 409344, 409635. Nonsense mutation around 409344, 409635.", "psc": { "uniref90_id": "UniRef90_UPI001E41E527", "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E41E527", "UniRef:UniRef50_UPI001E41E527" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527" ], "product": "MSC_0624 family F1-like ATPase-associated membrane protein" } }, "id": "LMJBGEANGM_728", "locus": "GCA013368015_00717" }, { "type": "cds", "sequence": "contig_1", "start": 409984, "stop": 410331, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGACTTACTTTATATAATAACAGTGAAGCATTCAATATATCAGGTGAAGAATTAAATGAAGCAGGCTTTAATAGACATATTGTTTCAGGCGAACCTGGTCAAAAAAGCAAGATTTTATTTAGTAATGGAAATTTAACAACAAAAGTAAGAATCACAGGAACTTCATTACTAAATTCAGAAGGAATTGACAACCTTTCGCGTTTATATATGTTTGCAGAACAATTGAAAAAAGAAATAATTGTTGATAATAATGCCTCAGCTTTAAAATCTCAATTAGAAGGTTTGGGATATAGTGTAAAAACCTCTTCTTCTTCTGATTCTGATGGAGAATTTATTTAA", "aa": "MIRRLTLYNNSEAFNISGEELNEAGFNRHIVSGEPGQKSKILFSNGNLTTKVRITGTSLLNSEGIDNLSRLYMFAEQLKKEIIVDNNASALKSQLEGLGYSVKTSSSSDSDGEFI", "aa_hexdigest": "fe26518889725d39460cec536a931f09", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12620.94469999999, "isoelectric_point": 5.386767768859863 }, "id": "LMJBGEANGM_729", "locus": "GCA013368015_00718" }, { "type": "cds", "sequence": "contig_1", "start": 410716, "stop": 410901, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATCAGACTCTAGCCAAAAATTTATGGATATTTTAAAGGCAATTCCTGATGAAATTCAACTACTAGAACTATTTTTCTCAGCAGGTTCTTCAAACACTTCTTCTTTAATTGCGTTAGAAAATAAACACATAAAGGAACTAGGAATTTACACTTTAGGAAATTCGCTATTGGATAAATGA", "aa": "MGKSDSSQKFMDILKAIPDEIQLLELFFSAGSSNTSSLIALENKHIKELGIYTLGNSLLDK", "aa_hexdigest": "f763aab2bca3814fe56dea6fe263b2ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6701.625999999998, "isoelectric_point": 5.082394981384277 }, "id": "LMJBGEANGM_730", "locus": "GCA013368015_00719" }, { "type": "cds", "sequence": "contig_1", "start": 411127, "stop": 411393, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCTCATTTAGATTTCTCTAAATTTACAAAGATTACTTCTAATGCTGAAAAGCAATTAGCTGAAGGCCTTGCATTAGATCAAGATAACGCCTATGTAAACGAAAAAGGTGAGCTAGATTCATATGCATATTCTCCAGCCAAAGGCTATAATGGTGTTGCAAATAGAATAATAAACGACAATAAAAATAGAAGAGTTTTTGGATATGATAGTATTGAAAATAGAAGCCCATATGGTCTTGCTAATGGCGACTATCCTGGTTGA", "aa": "MYSHLDFSKFTKITSNAEKQLAEGLALDQDNAYVNEKGELDSYAYSPAKGYNGVANRIINDNKNRRVFGYDSIENRSPYGLANGDYPG", "aa_hexdigest": "a74f8834f7055dc56c4ed59ec082d28e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9832.597499999996, "isoelectric_point": 5.229266929626463 }, "id": "LMJBGEANGM_731", "locus": "GCA013368015_00720" }, { "type": "cds", "sequence": "contig_1", "start": 411601, "stop": 412290, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACTTATTAAAGAAAAGAAAAAACAAATTAATTTTAATGACGGCAGGTGTTGTTATAGGAGCTTCAATAATTGCTGGGGCTATATTTTATAGTACAAGTTCGCAAAATTATTCTCGTTTTAATACCACCAGCTCAGCAACAGGAAATGATTTTATAAATAAAAGCAACCTAGATTATAAAAATGCGTTGCCTTCAATAAAGGATAACAACTTAAAAGAATTAGAAAAGCCACAACCAATTCCAACCCCTAAACCAGAGCTTGAACCAATCCCAGTTCCGCCGGCTCCTAAACCAGAGCCAAAACCAAAACCCGAACCTAAGCCAGAGCCAAAACCTCAACCTCAACCAACCCCTCCAGCTCCAAAACCAGAGCCAAAACCAAAACCCGAACCTAAGCCTACTCCCCCTTCATCAAAACCTTCAACTAGAACAACTGAAATTATTCTAAACGGTGTCAAAGTTGAAGCGGAAGTTGAAGATCAAAAGAAACGTGAATATAATCAATATGATATCTTAAATCATCTAACTAATTTAAACCCATACATCAATGATAGTGCTCCAAAAATTAAAAAAGTTAAGGTTACTGAAGAATTGAAGCAAGCAGCAGCCCAAGCGGCTACTCAACGTTTAAAAAGAGATGAAAAATACTTCAAAAAAGATCTTGAAGATTATGAAAAAAGTTGA", "aa": "MKNLLKKRKNKLILMTAGVVIGASIIAGAIFYSTSSQNYSRFNTTSSATGNDFINKSNLDYKNALPSIKDNNLKELEKPQPIPTPKPELEPIPVPPAPKPEPKPKPEPKPEPKPQPQPTPPAPKPEPKPKPEPKPTPPSSKPSTRTTEIILNGVKVEAEVEDQKKREYNQYDILNHLTNLNPYINDSAPKIKKVKVTEELKQAAAQAATQRLKRDEKYFKKDLEDYEKS", "aa_hexdigest": "3302c66c69f0a5e7fce54e36faa85bdf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 25647.12660000001, "isoelectric_point": 9.549073219299316 }, "id": "LMJBGEANGM_732", "locus": "GCA013368015_00721" }, { "type": "cds", "sequence": "contig_1", "start": 412320, "stop": 412796, "strand": "-", "frame": 1, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "GTGTTTTTAGCACAACCAGGTGGAATGAAGCAAGTTCAAGAAGAAATAAATGGCCAAAAATATCAAAAATCAGTATTTTCATTTTATAAAAGTAGCGATGGCAAAACATATATGCGTATGGGGCTAAATTATATGCCTAAACATTTTGCTCCAGTTGGTGGTGCTTCTGGTACAAGCTTAAGAAATCAAAATAACGAACTAGTTGGAATATTTTATTTATCAAATTTAGGTGCAAAAACCGGTTTAGCAGCTGCTTTTAGATCGGAAGGATTTGACTACAAAGGCTTGTATGGAAATTACAATTTGCCTCAATATGATTTAATTTATGGCGGCGGTAAAGACCAAAAAAATTCATTTAGACAAACATTAAAAGAACTATATAAAAATAATAGCGATTTTAAAACAGCATTATTCCCTAATGGCTTAGGTGATGATAAAGTTCCTAATGAATTTAAATTTATAGAAAATAAAAAATAA", "aa": "MFLAQPGGMKQVQEEINGQKYQKSVFSFYKSSDGKTYMRMGLNYMPKHFAPVGGASGTSLRNQNNELVGIFYLSNLGAKTGLAAAFRSEGFDYKGLYGNYNLPQYDLIYGGGKDQKNSFRQTLKELYKNNSDFKTALFPNGLGDDKVPNEFKFIENKK", "aa_hexdigest": "4adebeadab6f4a5cad1f0fd8f83c0609", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CCEF0F", "query_cov": 0.8164556962025317, "subject_cov": 0.9626865671641791, "identity": 0.519, "score": 141.0, "evalue": 2.2e-40, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "LMJBGEANGM_733", "locus": "GCA013368015_00722" }, { "type": "cds", "sequence": "contig_1", "start": 413067, "stop": 413522, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGGATAGTGCAAAAATTAAAACAACATTGCGTTTAGCAAATCTTGATGAAAATACATATAGATTTTCATTTACTGGTAAAGATTCTACTAATGTTTCTAATGGAATAAAGACAGTTTATACTGCAAGAGATTTCTTAAATTTAAAACCTTCTGATTATTTAATTAAATCACAAAAAGAAAAGTATAAAGATGTTGAAGAATATGCTGATTTTGCAGTTTTTGAAGTTGATTTTGAAAAATTAACATTACAAAGCGTTTCTAAAAATGATCTAAGCGAAAATAGCGATATTACTCAAAAATTCAGCAATTTAGATGCCCATGAATTAGCAAAAGCCTTGACAAGTAATTATGCTCAAAATAAAGCAAAACAAATTAAATTCTTGTCGAAATCATATTTATCAAATTACCAAAAAATTGATGTTCCTTTAAAGGCAAATAAAGCAAATAAGGAATGA", "aa": "MDSAKIKTTLRLANLDENTYRFSFTGKDSTNVSNGIKTVYTARDFLNLKPSDYLIKSQKEKYKDVEEYADFAVFEVDFEKLTLQSVSKNDLSENSDITQKFSNLDAHELAKALTSNYAQNKAKQIKFLSKSYLSNYQKIDVPLKANKANKE", "aa_hexdigest": "1e87265dcc742aa16c7a4f0bae8aba26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 149, "aa_cov": 0.9271523178807947, "hmm_cov": 0.8322147651006712, "evalue": 3.2710060386411984e-25, "score": 84.98310852050781, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 17273.178699999997, "isoelectric_point": 8.872670936584473 }, "id": "LMJBGEANGM_734", "locus": "GCA013368015_00723" }, { "type": "cds", "sequence": "contig_1", "start": 413643, "stop": 414251, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTTAATATTACTATTAAAGACAAGAAGACAAACAAAACATTTATTGAAACAATAACTGTAGGAAAATTTAAAACAAATCCTTATGGTTTTGATGAAAATGGAACAATTCCTAATACAGGTTCTGATGCTTTAAGATTGAATAAAAGTGAGTTGGAACAATATCTTGATGGAAATCAAACAAAAAGATATGAGCTAGATAATAAAAAATATTTAGACATTTTAACTCGTCAACAAGAACAAAAATCAATAAAAGAAATTAGACCAAATTTGGATGCTTCACAATCTAACATTCAAAAATTTGATGCTAAGGCAAAAGAAATTAATCAAGATTCGTATGAATCAGCTGCAAGAAAAGGTTTTACATTGCCAAGCTACGATTCAAAAGGCAATGTAGAAGGCCTTGCTATTAATGAAACTCCAGGAGGTCAAGGGCCTTCACCTACAGATGCATTTGGTAGAGATCCATATAAAATTACAGGTTTAGCAAGAACATTGCCAAATGAACATTACCGTGACATTGCTTCACAAACATTTGGAATAAGATTCTTAACTCCTATTGAAGGCGAAGAAAGAAAATTTACTAATACAGCTGGAACAACTTGA", "aa": "MNFNITIKDKKTNKTFIETITVGKFKTNPYGFDENGTIPNTGSDALRLNKSELEQYLDGNQTKRYELDNKKYLDILTRQQEQKSIKEIRPNLDASQSNIQKFDAKAKEINQDSYESAARKGFTLPSYDSKGNVEGLAINETPGGQGPSPTDAFGRDPYKITGLARTLPNEHYRDIASQTFGIRFLTPIEGEERKFTNTAGTT", "aa_hexdigest": "f26d39279399217cd1e027a1a2955c2c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22713.921399999992, "isoelectric_point": 7.787216758728027 }, "id": "LMJBGEANGM_735", "locus": "GCA013368015_00724" }, { "type": "cds", "sequence": "contig_1", "start": 414276, "stop": 414746, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAATCTAAATTAATAATATCATTATTAATAGCAGCGACATCACTTTCTAGTATGTCTGCTGTAGCTATTTCATGTAAAAATACAAATAAAAAGACTAATAATAATCAAAATGACGAAAAAAAGAATCCTGATGAAAAACCCCAAAATAATCAAGATTCAAAAGGCAACGACAACAAAAATAATAATTCTACAGATGAACTATCAGTTGAAGAACAAAAAACTACCGAATATAAAAAAATAATTTCTGAAGTTTCAAAATTAGCATTAAATGATCCAAGTCAATTATTTGATTTGAAATTCAAGGCTTTTTCAAATCAAAATTCACAAGATTTGCTTCCATCTCAAATTGTTAGAAATTTCAATCAAGGTGTTGATTTTAAACAAAAAAAATTTAATGATGAAATTGAAATTAAAAAAATAGTCAATGTTTTATACGTACAAACTTCAACCAATTCTGATGATTGA", "aa": "MKKSKLIISLLIAATSLSSMSAVAISCKNTNKKTNNNQNDEKKNPDEKPQNNQDSKGNDNKNNNSTDELSVEEQKTTEYKKIISEVSKLALNDPSQLFDLKFKAFSNQNSQDLLPSQIVRNFNQGVDFKQKKFNDEIEIKKIVNVLYVQTSTNSDD", "aa_hexdigest": "77acd8f186d5bd0a51ebfa2ff9f09f21", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17619.437500000004, "isoelectric_point": 8.525508689880368 }, "id": "LMJBGEANGM_736", "locus": "GCA013368015_00725" }, { "type": "cds", "sequence": "contig_1", "start": 415058, "stop": 415444, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V8AMI9" ], "nt": "ATGTTGGATGTTTGCAATTTAGCTAAAGTTTTTCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAATGGGGTTGGAAAATCCACTTTTTTAAAAATATTAAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGATGATTATATCGCAACTGATGTTGTAATAATGGGAAATAAAGAACTTTATCAAATTAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAAAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGA", "aa": "MLDVCNLAKVFPDKKLFTNVNLKFLPGNVYGIIGANGVGKSTFLKILSGDIEASEGQIIKEKNARMSVLSQNQNEFDDYIATDVVIMGNKELYQINLEKTKIYEDPNATIQDYEKASHLEEKFGEMGG", "aa_hexdigest": "c70b676939e47c862fa354f4f78ad32e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0011CB74F5", "query_cov": 0.8359375, "subject_cov": 0.9727272727272728, "identity": 0.514, "score": 108.0, "evalue": 2.01e-28, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_V8AMI9" }, "pscc": { "uniref50_id": "UniRef50_V8AMI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V8AMI9" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_737", "locus": "GCA013368015_00726" }, { "type": "cds", "sequence": "contig_1", "start": 415508, "stop": 415636, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCGGATTTAAAAGCCAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCTGATATTTTAGTAATGGATGAACCAACAAACAGATTGGACCTAAAAACAATTAAGTGA", "aa": "MKMSDLKANEKVKVLLAKALFGNPDILVMDEPTNRLDLKTIK", "aa_hexdigest": "767e909f7a3a553f1973341b68c98a0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.6662, "isoelectric_point": 9.22402362823486 }, "id": "LMJBGEANGM_738", "locus": "GCA013368015_00727" }, { "type": "cds", "sequence": "contig_1", "start": 415652, "stop": 415777, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATCCAAATATTGTTATTGTTGTTTCTCACGATAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGATATAGATTACTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGA", "aa": "MDYPNIVIVVSHDSDFLDAICTHIIDIDYSEAKLFIGNYSF", "aa_hexdigest": "459d1e9fe7647d18e60ab69fb0c47a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4695.236499999998, "isoelectric_point": 4.0500284194946286 }, "id": "LMJBGEANGM_739", "locus": "GCA013368015_00728" }, { "type": "cds", "sequence": "contig_1", "start": 415805, "stop": 416194, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF12848.13" ], "nt": "ATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCCACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCCTCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCCGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGATACTTTATTTGAAAATGTAAGTTTTACAATCCACCCTGGAGAAAAAATGGTTATTATTGGCAATGATGACATTGCCAAAACTAGATTACTTGAAATACTTATGGGTAAGCGTAAACCAACATCAGGAACAGTTGAATGA", "aa": "MQQKQNLKKEEIAQKLKDFIARFSANASKSKQATSRKKLLDKIVIDEIKPSSRKYPFIQFNLNRTPGKQILTVKNLTYVNDNGDTLFENVSFTIHPGEKMVIIGNDDIAKTRLLEILMGKRKPTSGTVE", "aa_hexdigest": "a837073c1b585038c050706acd779c73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 41, "aa_cov": 0.3178294573643411, "hmm_cov": 1.0, "evalue": 7.412343690053465e-08, "score": 28.639638900756836, "start": 88, "stop": 128 }, { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 59, "aa_cov": 0.4573643410852713, "hmm_cov": 0.9661016949152542, "evalue": 4.659870908327764e-13, "score": 44.65205001831055, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 14693.900499999978, "isoelectric_point": 9.908742332458495 }, "id": "LMJBGEANGM_740", "locus": "GCA013368015_00729" }, { "type": "cds", "sequence": "contig_1", "start": 416333, "stop": 416671, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAAAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATTTTCGACCAACCTCTTGACCACCTTGATAGTGAATCTATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGAGCAATGATAAAAGAATGTGCCTCAGTTATTTTAGAAATAAAGCCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAA", "aa": "MRAFLGRMLFSNDSVFKNVNVTSGGEKVRLMMAKLMLEESNFLIFDQPLDHLDSESIDSLIEAIKNYKSSCIFTTYNRAMIKECASVILEIKPTSSYLFYGTLEEYEEAMGY", "aa_hexdigest": "09499d814b522967d8a94203f3f165be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12834.661299999987, "isoelectric_point": 4.819230461120605 }, "id": "LMJBGEANGM_741", "locus": "GCA013368015_00730" }, { "type": "cds", "sequence": "contig_1", "start": 416715, "stop": 416975, "strand": "-", "frame": 1, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6" ], "nt": "ATGTTTTATTATTTTTTTAACTTTTCAAATGCCTTAAAGCAAAGATATGAAAACGTAAATTTACCCTTAATTAAAAAAGAAGAACTTGGGAATTTATTATTCCCCATTCCCCACATTTCAATTCAAAATAAAATAGTTGAAATTCTTGACAAACTTGAAACATATACCAAAGATATTCAATCCGGCCTTCCTCTTGAAATTGATCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGCTAAACTTTAAAAAGTAA", "aa": "MFYYFFNFSNALKQRYENVNLPLIKKEELGNLLFPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLNFKK", "aa_hexdigest": "96ea49736a0a0371d6d8780c4761a49c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6A8PYV0", "query_cov": 0.9767441860465116, "subject_cov": 0.8076923076923077, "identity": 0.69, "score": 110.0, "evalue": 5.87e-30, "valid": false, "product": "Restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AAC9T2H6", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6" ], "product": "Type I restriction modification DNA specificity domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_742", "locus": "GCA013368015_00731" }, { "type": "cds", "sequence": "contig_1", "start": 417359, "stop": 417613, "strand": "-", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00249DF03A", "UniRef:UniRef90_UPI0030B97C6F" ], "nt": "ATGCAGACAAAAAAATTTGTAAACAAGGCAAGTGGAAATCCTAAACTTATGAGTAATATTATGGAAAATATTGAAATCCCTATCCCTCACATTTCCATCCAAAATAAAATTGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGATCAACATAAAAAGCAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGGTATTAA", "aa": "MQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQHKKQYEYYRDKLLDFKDLAGGGY", "aa_hexdigest": "a853b7749399c27cf18621e1aa7015af", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030B97C6F", "query_cov": 0.8214285714285714, "subject_cov": 0.8846153846153846, "identity": 0.971, "score": 135.0, "evalue": 6.31e-40, "valid": true, "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI00249DF03A" }, "pscc": { "uniref50_id": "UniRef50_UPI00249DF03A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00249DF03A" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "LMJBGEANGM_743", "locus": "GCA013368015_00732" }, { "type": "cds", "sequence": "contig_1", "start": 417746, "stop": 417895, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTGTGAAATAAAGCGAGGAAAAGTTTACTCAAAGGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAAACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGA", "aa": "MCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEYVT", "aa_hexdigest": "b0f70c5bb8111e7c075e0d3502c6a325", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 1.0, "hmm_cov": 0.9811320754716981, "evalue": 2.2658264595821623e-09, "score": 33.23988342285156, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5670.380600000001, "isoelectric_point": 6.121525382995604 }, "id": "LMJBGEANGM_744", "locus": "GCA013368015_00733" }, { "type": "cds", "sequence": "contig_1", "start": 417969, "stop": 418508, "strand": "-", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00131480C8", "UniRef:UniRef90_UPI00131480C8" ], "nt": "TTGTCATTTAAGCATGTTTCTGCAAATGAACTTAAGCTCTATAAAAAAGAAGAAACGGGAAATGTAAAATTATTATCAACAGGTCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCTTCTGGTGGCGCGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTATTTTTTAATTGCTAGCAAAGAAAACATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATTGAATTGCAAATTCCACTTCCTCATATTTCGATTCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATACACCAAGGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGCAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGTATTAAGTAA", "aa": "MSFKHVSANELKLYKKEETGNVKLLSTGQYDGFTSYVENDKNINYGEIISIPSGGAPLIKYYKGYFIDSLNILLSSKDVKKYSLKFLYYFLIASKENIEQNFRGSSIKHPDMENIIELQIPLPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGGIK", "aa_hexdigest": "78c61bdd913a6319b6ecd970c8ef1a29", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131480C8", "query_cov": 0.9832402234636871, "subject_cov": 0.8036529680365296, "identity": 0.977, "score": 345.0, "evalue": 1.85e-119, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00131480C8", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI00131480C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00131480C8" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "LMJBGEANGM_745", "locus": "GCA013368015_00734" }, { "type": "cds", "sequence": "contig_1", "start": 418569, "stop": 419291, "strand": "-", "frame": 1, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4V6YSE1" ], "nt": "ATGAACAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAAAAAATTGGAGATTTAGGCATTCTATATAATGGATTGTCAGGCAAAAATAAAAATGATTTTTTGAATAATGCCAATAAACAATATATTACATATTTAAATATTTTTAACAATTTATCTATTGATATTAAAAGTTTAGAAAAAGTTTCCGTATTAAAAAATGAGAAGCAAAATAGAGTGTTATATGGAGATATTCTTTTTACTACTTCATCCGAAACTGCAAATGAGTGCGGTTACGCTTCTGTAGCTAATGATAAATATTTCGACAATAATGATGTTTATTTAAATAGTTTCTGTTTTGGTTATAGACTATTCAATATCGAAAATTATAATGTAAATTATTTTAAATATTTATTTAAGGATTTAAATATAAGAAAGGAAATAATTAAATGTGTTAATGGAGTAACTAGATTTAATTTATCAAAAGAGCAATTTAAAAGAATTTTAATTCCAATTCCCCCTCTTGAAATCCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAAATAAACAATATAATTACTATAGAAATAAATTATTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAAACAACAATGCAGTAATAGTATAGTTGAATATAAGAGGTTATGA", "aa": "MNKIEKLIKELCPNGVEYKKIGDLGILYNGLSGKNKNDFLNNANKQYITYLNIFNNLSIDIKSLEKVSVLKNEKQNRVLYGDILFTTSSETANECGYASVANDKYFDNNDVYLNSFCFGYRLFNIENYNVNYFKYLFKDLNIRKEIIKCVNGVTRFNLSKEQFKRILIPIPPLEIQNQIVNILDKFTELTTELTTELTYRNKQYNYYRNKLLDFDNNKELLNKIMNKQQCSNSIVEYKRL", "aa_hexdigest": "215194053b2ea245bc23ecb674329a30", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A2C6AXK9", "query_cov": 0.9458333333333333, "subject_cov": 0.9700854700854701, "identity": 0.551, "score": 228.0, "evalue": 7.86e-72, "valid": false, "product": "Restriction endonuclease subunit S", "uniref50_id": "UniRef50_A0A4V6YSE1" }, "pscc": { "uniref50_id": "UniRef50_A0A4V6YSE1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4V6YSE1" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "LMJBGEANGM_746", "locus": "GCA013368015_00735" }, { "type": "cds", "sequence": "contig_1", "start": 419294, "stop": 419758, "strand": "-", "frame": 2, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACATGTATAATGGTATTGAAAAAATCAAAAATTGACAACAATATCTTATTTATTGATGCCTCTCAAGAATTTTTGAAAGTAACAAACAACAACAAATTAACTAGTCAAAATATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCAAAATTAGCCAGCGTTGAAGAAATTAGGTCCAATTCATATAATTTATCAGTCAATAGTTACGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGCGAAATTGACAAAATAGTTGCTGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQRKDISYISKLASVEEIRSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "441608ae21f2f50f71593e9733556100", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.66, "score": 200.0, "evalue": 2.11e-63, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 419294, "stop": 420058, "observations": { "mutation": [ 419806, 419917 ], "stop": [ 419806, 419917 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.987, "score": 281.0, "evalue": 5.26e-93, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.984251968503937, "score": 488.0, "evalue": 9.22e-177, "description": "Internal stop codon at 419806, 419917. Nonsense mutation around 419806, 419917.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "LMJBGEANGM_747", "locus": "GCA013368015_00736" }, { "type": "cds", "sequence": "contig_1", "start": 419915, "stop": 420742, "strand": "-", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAACGTCAAGCAGGAATTGAAGATTTTGATTATACAACTTTATCAGATGAAGAAGCATTGACTGGAAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAGAAATGCTACAACAAATAATTGTCTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAGGGACAACAAAGTGAAAATGATTTTTCAGGTCTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTATCAGGATAATTCAATAGATGCCTTTGGTGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCCGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCAGAATTGCTTACTAAAATAGCAGTTTTCAATAAGAAAAAAGTTAATAGAGTATATGACCCCGCATGTGGATCGGGTTCTTTACTTCTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGTCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTATAACGGCGACACATTGCTATCTCCAAGTCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQRQAGIEDFDYTTLSDEEALTGRDDLINEKGFFILPSELFINVVRNATTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "84a04d1b79b4b94b47e206e06b4a5ce5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.662, "score": 368.0, "evalue": 3.84e-125, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "LMJBGEANGM_748", "locus": "GCA013368015_00737" }, { "type": "cds", "sequence": "contig_1", "start": 421048, "stop": 421602, "strand": "-", "frame": 3, "gene": "yajL", "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_114095983.1", "SO:0001217", "UniParc:UPI000DEE009A", "UniRef:UniRef100_A0A6A8PZD9", "UniRef:UniRef50_D1J8B4", "UniRef:UniRef90_D1J8B4" ], "nt": "ATGAATTTATTAGTAATTGTTGAAGAAGGATTTGAAGATACTGAATTAACAGGAACATTAGATTTATTTCATAGAGCAAATAATTTATTTAATAAGACCACATTTTACAATCCTCTAGGAATTGAAGCAACTGGTGCACATAAAATTGTAAAATTGCAAACAACTTCAAATATAAATGTTGATGAATATGATGTTTTATTTATTCCTGGAGGACCCGCTGCTAAAAGTATTAGAAAGAACCAAACATTTTTAAACTTAATAGCTAAGTTTAAAAATCAAAACAAATTAATTATAGCAATATGCGATGCTCCCAATGTTTTATATGAAAATAAGTTTATTGATAGTGAAACTAAATTTAGTTCATTTCCAATAAACGACAGTGTAAAAGAAATATCTAATCGTAGTAAAGAATTAGTATCAATAAGCCCAGATAAAAAATTAATAACAGGAAAATGCGCGGCTGCTGTTTTTGATTTTGCATTAGCTGTTATTGAAGTTTTGAGAGGCAATCAACTTGCTAAATTAGTTCTTCAAGCAATAAAACCTATTGATTAA", "aa": "MNLLVIVEEGFEDTELTGTLDLFHRANNLFNKTTFYNPLGIEATGAHKIVKLQTTSNINVDEYDVLFIPGGPAAKSIRKNQTFLNLIAKFKNQNKLIIAICDAPNVLYENKFIDSETKFSSFPINDSVKEISNRSKELVSISPDKKLITGKCAAAVFDFALAVIEVLRGNQLAKLVLQAIKPID", "aa_hexdigest": "b10c6f674cb758d513efb235c6ee04f2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000DEE009A", "ncbi_nrp_id": "WP_114095983.1", "uniref100_id": "UniRef100_A0A6A8PZD9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DEE009A", "RefSeq:WP_114095983.1", "UniRef:UniRef100_A0A6A8PZD9" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PZD9", "uniref90_id": "UniRef90_D1J8B4" }, "psc": { "uniref90_id": "UniRef90_D1J8B4", "gene": "YajL", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_D1J8B4", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_D1J8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B4" ], "product": "DJ-1/PfpI domain-containing protein" }, "genes": [ "yajL" ], "id": "LMJBGEANGM_749", "locus": "GCA013368015_00738" }, { "type": "cds", "sequence": "contig_1", "start": 421755, "stop": 421934, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "ATGCCTGGTTTATATATTAATATTCATAAATTAACAATTCATATGAATTCAAATCCTAAAACAATTGAAATGGATTTTAGCTTAAAATATTCAAAATCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "084e7b801b313560ac8c22a03bd1e288", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 421755, "stop": 422102, "observations": { "mutation": [ 421943 ], "stop": [ 421943 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 1.0, "subject_cov": 0.5086206896551724, "identity": 0.983, "score": 120.0, "evalue": 6.01e-34, "gene_start": 1, "gene_end": 59, "reference_start": 58, "reference_end": 116, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9051724137931034, "score": 213.0, "evalue": 3.66e-73, "description": "Internal stop codon at 421943. Nonsense mutation around 421943.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7218.2438, "isoelectric_point": 7.9542543411254885 }, "id": "LMJBGEANGM_750", "locus": "GCA013368015_00739" }, { "type": "cds", "sequence": "contig_1", "start": 421941, "stop": 422120, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI001939F8FF" ], "nt": "TTGATTCCTAAAAAAGTTTTACTCGAAGCAAAATTTATTGATAATAAAAATGTTGATTTAAAAGAAGCATTCTTTAAATATAATTCATTATATCGCAACTCTAAAACTAAAATTTATGCAAATTTAAAAAGTGGTGATATCACAATTAAAAATTTTGAATTAGGTGCTGCAAAAGATTGA", "aa": "MIPKKVLLEAKFIDNKNVDLKEAFFKYNSLYRNSKTKIYANLKSGDITIKNFELGAAKD", "aa_hexdigest": "8538b9d30941fade6a4e0c3469998177", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 421755, "stop": 422177, "observations": { "mutation": [ 421943, 422174 ], "stop": [ 421943, 422174 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939F8FF", "query_cov": 1.0, "subject_cov": 0.42142857142857143, "identity": 0.983, "score": 115.0, "evalue": 5.72e-32, "gene_start": 1, "gene_end": 59, "reference_start": 20, "reference_end": 78, "reference_sequence": "YWILDWFTLEDKEFNCIFFLIPKKVLLEAKFIDNKNVDLKEAFFKYNSLYRNSKTKIYANLKSGDITIKNFELGAAKDWKEMPGLYINIHKLTIHMNSDPKTIEMNFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9571428571428572, "score": 271.0, "evalue": 9.25e-96, "description": "Internal stop codon at 421943, 422174. Nonsense mutation around 421943, 422174.", "psc": { "uniref90_id": "UniRef90_UPI001939F8FF", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939F8FF", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6798.856900000001, "isoelectric_point": 9.60180835723877 }, "id": "LMJBGEANGM_751", "locus": "GCA013368015_00740" }, { "type": "cds", "sequence": "contig_1", "start": 422613, "stop": 422738, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCCGGTGCATAAATTAGATGATAAGAATAGTAAACCCGAGTTGCGCTTTTTTTACATTAAACAATTAATGTTAGATTGCAGTCTAAACAAAAAAAGACTTGAACTTGATGTTCATTTATGA", "aa": "MAPVHKLDDKNSKPELRFFYIKQLMLDCSLNKKRLELDVHL", "aa_hexdigest": "8c2aa8a6f7f079dc2dabd247e1d49fd9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4927.810099999999, "isoelectric_point": 8.93191738128662 }, "id": "LMJBGEANGM_752", "locus": "GCA013368015_00741" }, { "type": "cds", "sequence": "contig_1", "start": 422751, "stop": 423164, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EA444", "UniRef:UniRef90_UPI001E2EA444" ], "nt": "TTGCCATTATTTGTAACCTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGCAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAAAGAACAAGAATCAGTTTTAAATAACGACGCAACCATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCATATTCCATTTTGTTTTTAATTCCTAAAAAGGTATTATTAGAAACAGGATTTATTTCAAGCGAAGATTTTAATTTAGCAGACTTTTATAGTAAAAACCTTAATTTCGTGTTTAATGAACAACCTAGAAAAATTTCAACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILKEQESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKI", "aa_hexdigest": "8c4e4e65185b00426774311fcde29aed", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 422688, "stop": 423221, "observations": { "mutation": [ 422753, 423206 ], "stop": [ 422753, 423206 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EA444", "query_cov": 1.0, "subject_cov": 0.7611111111111111, "identity": 0.985, "score": 270.0, "evalue": 6.4e-91, "gene_start": 1, "gene_end": 137, "reference_start": 20, "reference_end": 156, "reference_sequence": "MKKNKWKLLTIFGISALPILPLLVTSCNNRETELKNAETIIGNKEIVFNMVDILKEQESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNSEPRLLLH" }, "paralog": false, "identity": 0.9661016949152542, "score": 343.0, "evalue": 3.38e-122, "description": "Internal stop codon at 422753, 423206. Nonsense mutation around 422753, 423206.", "psc": { "uniref90_id": "UniRef90_UPI001E2EA444", "uniref50_id": "UniRef50_UPI001E2EA444", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EA444", "UniRef:UniRef50_UPI001E2EA444" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EA444", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EA444" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16002.209899999994, "isoelectric_point": 7.665954399108887 }, "id": "LMJBGEANGM_753", "locus": "GCA013368015_00742" }, { "type": "cds", "sequence": "contig_1", "start": 423221, "stop": 423460, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_A0A6A8Q0U9" ], "nt": "ATGCCTGATCCGATGGGGAGAGGAAAAGTTGGTCATGCAATTGTTGCTTATGGAATGTATGACAATGGAAATAAACTTCTTTGCCATTATGGTTGGGGAGGATATTCTCAAGTAATTGTTTCTTCAAGTCTTTCTGGTCAGTTATTTTTATTGGGCATGAAGCCAAATGGAAAAGTTTATAAAACAAGGAAACATTTTATTAAAAACGGAATTAAATATTCGGGAACGGATTTTAAATAA", "aa": "MPDPMGRGKVGHAIVAYGMYDNGNKLLCHYGWGGYSQVIVSSSLSGQLFLLGMKPNGKVYKTRKHFIKNGIKYSGTDFK", "aa_hexdigest": "1fab90f0b16c11ab94c69a4c52392084", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A6A8Q0U9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.987, "score": 166.0, "evalue": 1.62e-52, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A6A8Q0U9" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_754", "locus": "GCA013368015_00743" }, { "type": "cds", "sequence": "contig_1", "start": 423632, "stop": 423814, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTATTATATGCTGAGTTTTTTCAAAATTATGGAGTCTTTTCAGATGACCAAGTGAAAAAATACTTTGTAGATTATCAGGATTCATCGGATTATTCAACATTTAAAAAATATGGCTATCCATATTCTCCTGCTATTTCTGATGAATTTGTTACAGATTTATGGACAAAACACGGATGA", "aa": "MLLLYAEFFQNYGVFSDDQVKKYFVDYQDSSDYSTFKKYGYPYSPAISDEFVTDLWTKHG", "aa_hexdigest": "4bb3a9178a0874eeb5bba70cbcd99aa9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7159.808000000001, "isoelectric_point": 4.465635108947754 }, "id": "LMJBGEANGM_755", "locus": "GCA013368015_00744" }, { "type": "cds", "sequence": "contig_1", "start": 424010, "stop": 424615, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C39-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U2", "UniRef:UniRef90_A0A454C9U2" ], "nt": "ATGTTTGAATTAATTGAATATATGCAAAAAATTGACAATTTCAATAAATTTGTTCAAAATAATGCAAAAAAAATAAATGAACTAAAAAAATATAATATTTCTGATGTAAAAGCTTATTTAAAGTTTTTGGAATTGGAAATATTTAAATACAATAGGAAAATTGATTATCAAATAAAAGATATGGAAATTATTTTTGACAATTTTGGTAATCCATATATTTATGTTAATTTTAATCCTTACGGATATATGGTTGTTTCGTTAATAAATAATGAAAGTGTCATCATTGATCCTTTGAATAAAAATGAAAATTTTAACAGTAGCGATTTTCATAAAAAATATTTTTTAGACTTAGATTCCTTAAGAATTCAGCCAAAAAATAACTCTTCTCAAATTCGTTCTTATCAAGAAAATTTTGTTTTAAAAAATTTCAAAGGTAAATATTACGACATTAGTGATAATTTAAATAATAAAAAATTAATAGAAGAAAAACAAAATTTAAAGTTTTCACTAAAAAAGTCTATTTCTTATAGAAATTCAAGCGAGGCTCATGAAATTAATACTGATGTTGAAATGATACATGCGGAAAAAGAAGTTCCGTATTCTTGA", "aa": "MFELIEYMQKIDNFNKFVQNNAKKINELKKYNISDVKAYLKFLELEIFKYNRKIDYQIKDMEIIFDNFGNPYIYVNFNPYGYMVVSLINNESVIIDPLNKNENFNSSDFHKKYFLDLDSLRIQPKNNSSQIRSYQENFVLKNFKGKYYDISDNLNNKKLIEEKQNLKFSLKKSISYRNSSEAHEINTDVEMIHAEKEVPYS", "aa_hexdigest": "a8173900f0d595b21a42e825a197c2db", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 423707, "stop": 424615, "observations": { "mutation": [ 423943, 424009, 424012 ], "stop": [ 423943, 424009, 424012 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U2", "query_cov": 1.0, "subject_cov": 0.4331896551724138, "identity": 0.995, "score": 386.0, "evalue": 1.39e-131, "gene_start": 1, "gene_end": 201, "reference_start": 1, "reference_end": 201, "reference_sequence": "MFELIEYMQKIDNFNKFVQNNAKKINELKKYNISDVKAYLKFLELEIFKYNRKIDYQIKDMEIIFDNFGNPYIYVNFNPYGYMVVSLINNESVIIDPLNKNENFNSSDFHKKYFLDLDFLRIQPKNNSSQIRSYQENFVLKNFKGKYYDISDNLNNKKLIEEKQNLKFSLKKSISYRNSSEAHEINTDVEMIHAEKEVPYSWWFKLAIKDKNFGYQSYSTINARWEKEKEEAKLRGDEGEAKFIQDKIDKYKCSDIHQGLCHYVALGMLLLYAEFFQNYGVFSDDQVKKYFVDYQDSSDYSTFKKYGYPYSPAISDEFVTDLWTKHGWGAIVTTSSYLKNVAYDFLKKPDLPNFWPVYVYRRSAGWIKPWKWIRDGYPCLIFGTYMPDPMGRGKVGHAIVAYGMYDNGNKLLCHYGWGGYSQVIVSSSLSGQLFLLGMKPNGKVYKTRKHFIKNGIKYSGTDFK" }, "paralog": false, "identity": 0.9867549668874173, "score": 600.0, "evalue": 2.63e-218, "description": "Internal stop codon at 423943, 424009, 424012. Nonsense mutation around 423943, 424009, 424012.", "psc": { "uniref90_id": "UniRef90_A0A454C9U2", "product": "Peptidase C39-like domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U2", "UniRef:UniRef50_A0A454C9U2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U2" ], "product": "Peptidase C39-like domain-containing protein" } }, "id": "LMJBGEANGM_756", "locus": "GCA013368015_00745" }, { "type": "cds", "sequence": "contig_1", "start": 424881, "stop": 425060, "strand": "-", "frame": 1, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAATAAAAAATATAATTTTGAATTCAAACTTGGCACAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCGCGAGGTTGCAAAAGTTTCTTTTAAAACAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGTSNKIIDLAANFILKYGQKTLREVAKVSFKTTIKALEKIDNKLE", "aa_hexdigest": "71d4886823d9787230cfde94a9c1aed7", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 424881, "stop": 425189, "observations": { "mutation": [ 425063, 425174 ], "stop": [ 425063, 425174 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 0.966, "score": 109.0, "evalue": 7.37e-30, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9509803921568627, "score": 183.0, "evalue": 1.1e-61, "description": "Internal stop codon at 425063, 425174. Nonsense mutation around 425063, 425174.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "LMJBGEANGM_757", "locus": "GCA013368015_00746" }, { "type": "cds", "sequence": "contig_1", "start": 425172, "stop": 425456, "strand": "-", "frame": 1, "gene": null, "product": "ribonuclease HIII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1B727", "UniRef:UniRef90_UPI0034A1B727" ], "nt": "GTGATAACAAATTTTTTGAGGTTATTTCATGAAAAAATAATTGAAATTTTTAATCAAATTGAAAATTTAGTTTATTATCAAAAAACTTACCTTAGTCAAAGTGGTTATAATAAATTAATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAATCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAATGTTGCAAAAGCATTTCAAAAAATTGCAAGCAATTTCTTGA", "aa": "MITNFLRLFHEKIIEIFNQIENLVYYQKTYLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAIS", "aa_hexdigest": "609489d0c92cb0cebd5d50b48347f87f", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 424908, "stop": 425510, "observations": { "mutation": [ 425063, 425174 ], "stop": [ 425063, 425174 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A1B727", "query_cov": 1.0, "subject_cov": 0.46534653465346537, "identity": 0.9890000000000001, "score": 180.0, "evalue": 4.67e-56, "gene_start": 1, "gene_end": 94, "reference_start": 21, "reference_end": 114, "reference_sequence": "VKKYKNSSVKNIPLEFFKFKVITNFLRLFHEKIIEIFNQIENLVYYQKTYLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKA" }, "paralog": false, "identity": 0.985, "score": 380.0, "evalue": 3.66e-137, "description": "Internal stop codon at 425063, 425174. Nonsense mutation around 425063, 425174.", "psc": { "uniref90_id": "UniRef90_UPI0034A1B727", "product": "ribonuclease HIII", "uniref50_id": "UniRef50_UPI0034A1B727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A1B727", "UniRef:UniRef50_UPI0034A1B727" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1B727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1B727" ], "product": "ribonuclease HIII" } }, "id": "LMJBGEANGM_758", "locus": "GCA013368015_00747" }, { "type": "cds", "sequence": "contig_1", "start": 425518, "stop": 425697, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI001939F8FF" ], "nt": "TTGCCTGGTTTATATATTAATATTCGCAAATTAACAATTCATATGAATTCAGATCCTAAAACAATTGAAATGGATTTTAGCTTAAAATATTCAAAATCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIRKLTIHMNSDPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "08e20477558867f16e3ea5b4de804f67", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 425518, "stop": 425952, "observations": { "mutation": [ 425706, 425949 ], "stop": [ 425706, 425949 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939F8FF", "query_cov": 1.0, "subject_cov": 0.42142857142857143, "identity": 0.966, "score": 115.0, "evalue": 5.72e-32, "gene_start": 1, "gene_end": 59, "reference_start": 82, "reference_end": 140, "reference_sequence": "YWILDWFTLEDKEFNCIFFLIPKKVLLEAKFIDNKNVDLKEAFFKYNSLYRNSKTKIYANLKSGDITIKNFELGAAKDWKEMPGLYINIHKLTIHMNSDPKTIEMNFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.8402777777777778, "score": 234.0, "evalue": 2.54e-81, "description": "Internal stop codon at 425706, 425949. Nonsense mutation around 425706, 425949.", "psc": { "uniref90_id": "UniRef90_UPI001939F8FF", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939F8FF", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7238.275, "isoelectric_point": 7.935687446594238 }, "id": "LMJBGEANGM_759", "locus": "GCA013368015_00748" }, { "type": "cds", "sequence": "contig_1", "start": 425704, "stop": 425886, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "TTGATTCCTAGAAAAGTTTTGTTCGAAGCAAAATTTATTGATAATGAAAATGTTGATTTAAAAGAAGCATTCTTTAAATATAATTCAGGATATTCGAACTTTAAAAAAACTAAAGTTTATGCAAATTTAAAAAGCGGTGAAATCACAATTAAAAACTTTGAATTAGGTGCTGCAAAAGATTGA", "aa": "MIPRKVLFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKD", "aa_hexdigest": "c99ae0f6042e4aa49d5068b8b0f8f5d8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 425518, "stop": 425868, "observations": { "mutation": [ 425706 ], "stop": [ 425706 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 0.9, "subject_cov": 0.46551724137931033, "identity": 0.981, "score": 108.0, "evalue": 1.64e-29, "gene_start": 7, "gene_end": 60, "reference_start": 1, "reference_end": 54, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9655172413793104, "score": 229.0, "evalue": 2.26e-79, "description": "Internal stop codon at 425706. Nonsense mutation around 425706.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6924.882300000001, "isoelectric_point": 9.260577201843262 }, "id": "LMJBGEANGM_760", "locus": "GCA013368015_00749" }, { "type": "cds", "sequence": "contig_1", "start": 426387, "stop": 426509, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCCGGTGCATAAATTAGATGATAAGAATAGTGAACCCAGGTTGCGCTTTTACATTAAACAATTAATGTTAGATTGCAGTCTAAACAAAAAAAGACTTGAACTTGATGTTCATTTGTGA", "aa": "MAPVHKLDDKNSEPRLRFYIKQLMLDCSLNKKRLELDVHL", "aa_hexdigest": "50ccf100106c5ad51b539f17e0f45702", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4808.649699999999, "isoelectric_point": 8.978850364685059 }, "id": "LMJBGEANGM_761", "locus": "GCA013368015_00750" }, { "type": "cds", "sequence": "contig_1", "start": 426522, "stop": 426935, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EA444", "UniRef:UniRef90_UPI001E2EA444" ], "nt": "TTGCCATTATTTGTAACCTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGCAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAAAGAACAAGAATCAGTTTTAAATAACGACGCAACTATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCAAATTCCATTTTGTTTTTAATTCCTAAAAAAGTATTATTAGAAACAGGATTTATTTCAATCGAAGATTTTAATTTAGCAGACTTTTATAGTAAAAACCTTAATTTCGTGTTTAATGAACAACCTAGAAAAATTTCAACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILKEQESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYANSILFLIPKKVLLETGFISIEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKI", "aa_hexdigest": "add900c1965dc3ef4f988a11b4b9c80c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 426459, "stop": 426992, "observations": { "mutation": [ 426524, 426977 ], "stop": [ 426524, 426977 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EA444", "query_cov": 1.0, "subject_cov": 0.7611111111111111, "identity": 0.971, "score": 265.0, "evalue": 3.04e-89, "gene_start": 1, "gene_end": 137, "reference_start": 20, "reference_end": 156, "reference_sequence": "MKKNKWKLLTIFGISALPILPLLVTSCNNRETELKNAETIIGNKEIVFNMVDILKEQESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNSEPRLLLH" }, "paralog": false, "identity": 0.9717514124293786, "score": 342.0, "evalue": 6.8e-122, "description": "Internal stop codon at 426524, 426977. Nonsense mutation around 426524, 426977.", "psc": { "uniref90_id": "UniRef90_UPI001E2EA444", "uniref50_id": "UniRef50_UPI001E2EA444", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EA444", "UniRef:UniRef50_UPI001E2EA444" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EA444", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EA444" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15979.21959999999, "isoelectric_point": 7.6720361709594735 }, "id": "LMJBGEANGM_762", "locus": "GCA013368015_00751" }, { "type": "cds", "sequence": "contig_1", "start": 426992, "stop": 427231, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_A0A6A8Q0U9" ], "nt": "ATGCCTGATCCGATGGGAAGAGGAAAAGTTGGTCATGCAATTGTTGCTTATGGAATGTATGACAATGGAAATAAACTTCTTTGCCATTATGGTTGGGGAGGATATTCTCAAGTTATTGTTTCTTCGAGTCTTTCTGGCCAATTGTTCTTATTAGGTATGAAACCAAATGGCGAAATTAAAAAAACAAGGAAGCATTTTATTAAAAACGGAATTAAATATTCGGGAACGGATTTTAAATAA", "aa": "MPDPMGRGKVGHAIVAYGMYDNGNKLLCHYGWGGYSQVIVSSSLSGQLFLLGMKPNGEIKKTRKHFIKNGIKYSGTDFK", "aa_hexdigest": "bab9233b7d061f7d36f03a093ddfa95b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A6A8Q0U9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9490000000000001, "score": 160.0, "evalue": 3.15e-50, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A6A8Q0U9" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_763", "locus": "GCA013368015_00752" }, { "type": "cds", "sequence": "contig_1", "start": 427403, "stop": 427585, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTATTATATGCTGAGTTTTTTCAAAATTATGGAGTCTTTTCAGATGACCAAGTGAAAAAATACTTTGTAGATTATCAGGATTCATCGGATTATTCAACATTTAAAAAATATGGCTATCCATATTCTCCTGCTATTTCTGATGAATTTGTTACAGATTTATGGACAAAACACGGATGA", "aa": "MLLLYAEFFQNYGVFSDDQVKKYFVDYQDSSDYSTFKKYGYPYSPAISDEFVTDLWTKHG", "aa_hexdigest": "4bb3a9178a0874eeb5bba70cbcd99aa9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7159.808000000001, "isoelectric_point": 4.465635108947754 }, "id": "LMJBGEANGM_764", "locus": "GCA013368015_00753" }, { "type": "cds", "sequence": "contig_1", "start": 427775, "stop": 428380, "strand": "-", "frame": 2, "gene": null, "product": "Peptidase C39-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U2", "UniRef:UniRef90_A0A454C9U2" ], "nt": "ATGTTTGAATTAGTTGAATATATGCAAAAAATTGACAATTTTAATAAATTTGTTCAAAATAATGCAAAAAAAATAAATGAACTAAAAAAATATAATATTTCTGATGTAAAAGCTTATTTAAAGTTTTTGGAATTGGAAATATTTAAATACAATAGGAAAATTGATTATCAAATAAAAGATATAGAAATTATTTTTGACAATTTTGGTAATCCATATATTTATGTTAATTTTAATCCTTACGGATATATGGTTGTTTCGTTAATAAATAATGAAAGTGTCATTATTGATCCTTTGAATAAAAATAAAGATTCTAACAGTAGCGATTTTCATAAAAAATATTTTTTAGACTTGGAATCCTTAAGAATTCAGCCAAAAAATAACTTTTCTCAAATTCGTTCTTATCAAGAAAATTTTGTTTTAAAAAATTTCAAAGGTAAATATTACGACATTAGTGATAATTTAAATAATAAAAAATTAATAGAAGAAAAACAAAATTTAAAGTTTTCACTAAAAAAGTCTATTTCTTATAGAAATTCAAGCGAGGCTCATAAAATTAATACTAATGTTGAAATGATACATGCGGAAAAAGAAGTTCCGTACTCTTGA", "aa": "MFELVEYMQKIDNFNKFVQNNAKKINELKKYNISDVKAYLKFLELEIFKYNRKIDYQIKDIEIIFDNFGNPYIYVNFNPYGYMVVSLINNESVIIDPLNKNKDSNSSDFHKKYFLDLESLRIQPKNNFSQIRSYQENFVLKNFKGKYYDISDNLNNKKLIEEKQNLKFSLKKSISYRNSSEAHKINTNVEMIHAEKEVPYS", "aa_hexdigest": "7e2d091d3566247b9f01e4d0a12cabad", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 427472, "stop": 428380, "observations": { "mutation": [ 427708, 427774, 427777 ], "stop": [ 427708, 427774, 427777 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U2", "query_cov": 1.0, "subject_cov": 0.4331896551724138, "identity": 0.95, "score": 370.0, "evalue": 2.29e-125, "gene_start": 1, "gene_end": 201, "reference_start": 1, "reference_end": 201, "reference_sequence": "MFELIEYMQKIDNFNKFVQNNAKKINELKKYNISDVKAYLKFLELEIFKYNRKIDYQIKDMEIIFDNFGNPYIYVNFNPYGYMVVSLINNESVIIDPLNKNENFNSSDFHKKYFLDLDFLRIQPKNNSSQIRSYQENFVLKNFKGKYYDISDNLNNKKLIEEKQNLKFSLKKSISYRNSSEAHEINTDVEMIHAEKEVPYSWWFKLAIKDKNFGYQSYSTINARWEKEKEEAKLRGDEGEAKFIQDKIDKYKCSDIHQGLCHYVALGMLLLYAEFFQNYGVFSDDQVKKYFVDYQDSSDYSTFKKYGYPYSPAISDEFVTDLWTKHGWGAIVTTSSYLKNVAYDFLKKPDLPNFWPVYVYRRSAGWIKPWKWIRDGYPCLIFGTYMPDPMGRGKVGHAIVAYGMYDNGNKLLCHYGWGGYSQVIVSSSLSGQLFLLGMKPNGKVYKTRKHFIKNGIKYSGTDFK" }, "paralog": false, "identity": 0.9276315789473685, "score": 562.0, "evalue": 2.09e-203, "description": "Internal stop codon at 427708, 427774, 427777. Nonsense mutation around 427708, 427774, 427777.", "psc": { "uniref90_id": "UniRef90_A0A454C9U2", "product": "Peptidase C39-like domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U2", "UniRef:UniRef50_A0A454C9U2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U2" ], "product": "Peptidase C39-like domain-containing protein" } }, "id": "LMJBGEANGM_765", "locus": "GCA013368015_00754" }, { "type": "cds", "sequence": "contig_1", "start": 428645, "stop": 428824, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAACAAAAAATATAATTTTGAATTCAAACTTGGTGCAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCGCGAGGTTGCAAAAGTTTCTTTTAAAACAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKALEKIDNKLE", "aa_hexdigest": "0084eb09148121a8e6683e2b4ad655e1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 428645, "stop": 428953, "observations": { "mutation": [ 428827, 428938 ], "stop": [ 428827, 428938 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 0.983, "score": 110.0, "evalue": 1.81e-30, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9607843137254902, "score": 185.0, "evalue": 2.73e-62, "description": "Internal stop codon at 428827, 428938. Nonsense mutation around 428827, 428938.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "LMJBGEANGM_766", "locus": "GCA013368015_00755" }, { "type": "cds", "sequence": "contig_1", "start": 428936, "stop": 429343, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B", "UniRef:UniRef90_UPI001F51E35B" ], "nt": "TTGAATAAAAACTTAGATGAATTAATTGGTGTTGATGAAACGGGAGTAGGAGATTATTTCAGTCCCGTGATTTCAGTTGCCTGTTATATACCAAAAGATAACATTGAAAAACTAAAACAAATTGGTGTTAAAGACAGCAAAAAACTTTCTGATAAAAGAATAATTGAAATTTTTAATCAAATTGAAAATTTAGTTTATTATCAAAAAACTTGCCTTAGTCAAAGCGGTTATAATAAATTAATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAACCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAATGTTGCAAAAGCATTTCAAAAAATTGCAAGCAATTTCTTGA", "aa": "MNKNLDELIGVDETGVGDYFSPVISVACYIPKDNIEKLKQIGVKDSKKLSDKRIIEIFNQIENLVYYQKTCLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAIS", "aa_hexdigest": "8ab1c7743e6c47e26e58a7dfe7cf5fda", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001F51E35B", "query_cov": 0.9259259259259259, "subject_cov": 0.9057971014492754, "identity": 1.0, "score": 246.0, "evalue": 2e-82, "valid": true, "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI001F51E35B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51E35B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B" ] }, "genes": [], "id": "LMJBGEANGM_767", "locus": "GCA013368015_00756" }, { "type": "cds", "sequence": "contig_1", "start": 429336, "stop": 429644, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGATATTACAACATTGACATTGATGATACTGTTGCAATGGGAGATTCATATAATGATTTAAGCATGTATAATGTTGCTAATGTTTGTGTATCACCTGCGAATGCTGAACCCTTAATTAAAAAGATGTCAACAGTTGTTATGAAGCAAACAAATAAAGAAGGCGCAGTCGGATACTTTATTGATCAATTCCTAAAAAACCCAGAAAAATACATTAAATTAGGTAAAAAACAAAAGGCCGAACAAAAAAATAATATTAAAACTGTAGGTGCAGATAACTTTTTTAGTAAAGAAGAAGAAATTGAATAA", "aa": "MRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE", "aa_hexdigest": "c8525aacea873a6f3ab319125237b291", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 60, "aa_cov": 0.5882352941176471, "hmm_cov": 0.9833333333333333, "evalue": 3.3709739996203886e-13, "score": 45.427085876464844, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 11611.095899999991, "isoelectric_point": 5.506129646301268 }, "id": "LMJBGEANGM_768", "locus": "GCA013368015_00757" }, { "type": "cds", "sequence": "contig_1", "start": 429723, "stop": 430118, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase in upp 3'region", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_P43051", "UniRef:UniRef90_P43051" ], "nt": "TTGCCTGTGTATGAAAAACTTGGACTTAATGCAATTGTAGGTAATTACAATGGTGCTCACATCCACAACCCTGCTGACCCATTCTTTATACCTGCTATTACATATTTGGACTTAAATGAAGTTCTTTATATTTTAGGCGATGAAAAAGTAAAAAAAGAAATTACTAATTATGCAATAGAAGGCCCTGACTGGGTTCAATTAATGCATAGAGATCCTAACCTTGAAAGAGTGTTTGGATTTAACCAAGCAACAAAATTTAGAGAATGTATTAACCTAGAAAAAATTCCACTAAAACCAACTGGAATTGTATTTGATGTTAAACCTGATACAGATGTTTTGGAATTATTGACATATTTAAAAAGACGTTATGGAGATTTAGGAGAATTTTCTTCTTGA", "aa": "MPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSS", "aa_hexdigest": "c77529f2e3482b857b9e30296c47109d", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "cof" ], "truncated": "both", "pseudogene": { "start": 429420, "stop": 430298, "observations": { "mutation": [ 429725, 430130 ], "stop": [ 429725, 430130 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43051", "query_cov": 1.0, "subject_cov": 0.409375, "identity": 0.992, "score": 275.0, "evalue": 2.73e-91, "gene_start": 1, "gene_end": 131, "reference_start": 61, "reference_end": 191, "reference_sequence": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRPWRSTLPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSSWSKGEGLSPVFDITSIGIDKGKVISLIMRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE" }, "paralog": false, "identity": 0.9931506849315068, "score": 580.0, "evalue": 7.14e-214, "description": "Internal stop codon at 429725, 430130. Nonsense mutation around 429725, 430130.", "psc": { "uniref90_id": "UniRef90_P43051", "gene": "Cof", "product": "Putative phosphatase in upp 3'region", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P43051", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43051", "EC:3.1.3.-", "UniRef:UniRef50_P43051", "COG:COG0561", "COG:HR", "KEGG:K21064", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P43051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43051" ], "product": "Putative phosphatase in upp 3'region" } }, "id": "LMJBGEANGM_769", "locus": "GCA013368015_00758" }, { "type": "cds", "sequence": "contig_1", "start": 430128, "stop": 430298, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGCGTACAACAAAGAATCAAAACAAAGAAGATTTTTATTTGCTATTGATCTAGATGGAACACTATTAGCAGATTCAGCCAATGGCACAGTTCATCCAAAAACAGAAGAAGCAATTAAAAAAGCTGTTGCCCAAGGACACATTGTTTCAATAATTACAGGAAGACCTTGA", "aa": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRP", "aa_hexdigest": "b4070446689c7714536aa9e25714399e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7678571428571429, "hmm_cov": 0.9534883720930233, "evalue": 7.756031458451415e-12, "score": 40.96463394165039, "start": 14, "stop": 56 } ], "seq_stats": { "molecular_weight": 6108.936900000001, "isoelectric_point": 9.30106334686279 }, "id": "LMJBGEANGM_770", "locus": "GCA013368015_00759" }, { "type": "cds", "sequence": "contig_1", "start": 430480, "stop": 431109, "strand": "-", "frame": 3, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206", "RefSeq:WP_020002490.1", "SO:0001217", "UniParc:UPI0003662176", "UniRef:UniRef100_A0A2K9YTA2", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_P43049" ], "nt": "ATGGTAAAAATTTTTCAGCATCCCTTAATTAATGCTAAATTAACAACGATGCGCTCAAAAGAAACATCTTACAAAGATTTTAGAGATAATTTGAACGAGATTGCTTCGTTGATGGTATATGAAACATTGAGGGACTATCAAACTAAGAAAATATCAATAACAACACCAATGAATGTTAAATATGAAGGTGAAACTCTTGATAGAGAAATTGTAATTATTCCTATTTTACGTGCGGGGCTTGGAATGTTGAATGGTATTATGGATTTAGTTCCTCAAGCTAGAGTTGGTCATATTGGAATGTATAGAAACGAAGAAACAAACGAAGTTGTTGAATATTTCTTTAAAATTCCAGAAGTACCACATGATAGCTATATTATTATTGTTGACCCTATGCTTGCAACCGGCTCATCTGCATGCGATGCAATAGCAAAATTGGATAAGTTAGGCTTTAGCAATATTAAATTAGTATGTTTAGTAGGCGTTCAACAAGGTATTGATAAAGTAACAAAACAATTTCCTAATGTTGATATATATTTAGCTTCAAAAGATGAAAAATTGAATGAACACAATTATATATTGCCTGGTTTGGGAGATGCTGGTGACCGTATATTTGGAACAAAAATAAAATAA", "aa": "MVKIFQHPLINAKLTTMRSKETSYKDFRDNLNEIASLMVYETLRDYQTKKISITTPMNVKYEGETLDREIVIIPILRAGLGMLNGIMDLVPQARVGHIGMYRNEETNEVVEYFFKIPEVPHDSYIIIVDPMLATGSSACDAIAKLDKLGFSNIKLVCLVGVQQGIDKVTKQFPNVDIYLASKDEKLNEHNYILPGLGDAGDRIFGTKIK", "aa_hexdigest": "3e720a22535f25de2c7be65caa1488af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003662176", "ncbi_nrp_id": "WP_020002490.1", "uniref100_id": "UniRef100_A0A2K9YTA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003662176", "RefSeq:WP_020002490.1", "UniRef:UniRef100_A0A2K9YTA2" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTA2", "uniref90_id": "UniRef90_P43049" }, "psc": { "uniref90_id": "UniRef90_P43049", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "LMJBGEANGM_771", "locus": "GCA013368015_00760" }, { "type": "cds", "sequence": "contig_1", "start": 431137, "stop": 431799, "strand": "-", "frame": 3, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_020002489.1", "SO:0001217", "UniParc:UPI000378BCA6", "UniRef:UniRef100_A0A2K9YTE4", "UniRef:UniRef50_Q98QP7", "UniRef:UniRef90_P43048" ], "nt": "ATGACAGAATTAAACAAATATATTGACCACACAAATTTATCACCTTCTGCAACAAGCAAAGACATTGATAAATTAATTCAAGAAGCAATTAAATATGATTTTAAATCAGTATGTATTGCACCATCATATGTTAAGTATGCTAAAGAAGCATTAAAAAACAGTGATGTGCTTGTTTGCACAGTTATTGGTTTTCCACTAGGCTATAATGCAACAAGTGTTAAAGTATATGAAACAAAAATTGCTGTTGAACATGGTGCTGACGAAATTGATATGGTAATCAATGTTGGAAGATTTAAAGATGGACAATATGAATACGTATTAAACGAAATCAAAGCAATAAAAGAAGCATGCAATGGCAAAACTTTAAAAGTTATAGTTGAAACAGCATTGTTAACAAAAGCTGAATTAATAAAAATTACTGAATTAGTAATGCAATCTGGCGCTGATTTTATTAAAACATCAACTGGATTTTCATATAGAGGAGCTTCATTTGAAGACATTCAAACTATGAAGGAAACCTGTGGTGACAAGCTTTTAATTAAAGCATCAGGTGGTATTAAAAATCTAGCAGATGCCCAAGAAATGATTAGATTAGGTGCAAACCGTTTAGGTATGTCTAAATCAGTTCCAGTTATGGAAGAATTATTTAAATTAGAAAAATAA", "aa": "MTELNKYIDHTNLSPSATSKDIDKLIQEAIKYDFKSVCIAPSYVKYAKEALKNSDVLVCTVIGFPLGYNATSVKVYETKIAVEHGADEIDMVINVGRFKDGQYEYVLNEIKAIKEACNGKTLKVIVETALLTKAELIKITELVMQSGADFIKTSTGFSYRGASFEDIQTMKETCGDKLLIKASGGIKNLADAQEMIRLGANRLGMSKSVPVMEELFKLEK", "aa_hexdigest": "58846acd5aac329a1c0a28ee84221e78", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000378BCA6", "ncbi_nrp_id": "WP_020002489.1", "uniref100_id": "UniRef100_A0A2K9YTE4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000378BCA6", "RefSeq:WP_020002489.1", "UniRef:UniRef100_A0A2K9YTE4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTE4", "uniref90_id": "UniRef90_P43048" }, "psc": { "uniref90_id": "UniRef90_P43048", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_Q98QP7", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP7" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "LMJBGEANGM_772", "locus": "GCA013368015_00761" }, { "type": "cds", "sequence": "contig_1", "start": 431826, "stop": 432050, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGAAATTGGGAGCAGGTAGATCTAAAAAAGAAGATTCTATCGACTATGAAGCAGGAATTTATTTGAATAAGAGTTCAAATGAATATGTAAATAAAGGAGATGTATTATTTACATTGTATTCGTCAAAACCTATAAATCCAGAACTTCAAAAAGAATTGTTGTCTGCAATAGAATTTAGCGAAAGCAAGCATGACATTCAAACGGTTTTTGCTAAATTAATGTAA", "aa": "MKLGAGRSKKEDSIDYEAGIYLNKSSNEYVNKGDVLFTLYSSKPINPELQKELLSAIEFSESKHDIQTVFAKLM", "aa_hexdigest": "5fc7271d4064027fce5b036a85fdb1be", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 60, "aa_cov": 0.7972972972972973, "hmm_cov": 1.0, "evalue": 7.699529110887937e-22, "score": 72.3613052368164, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8359.406099999997, "isoelectric_point": 5.74621753692627 }, "id": "LMJBGEANGM_773", "locus": "GCA013368015_00762" }, { "type": "cds", "sequence": "contig_1", "start": 432189, "stop": 432800, "strand": "-", "frame": 1, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "nt": "ATGTCGGGAAGAGGACTTGGACATACAGGAGGAACACTTGATAAATTAGAATCAATTGACGGTTATAATTTTTCAGTGACTGACCAACAATTTAGAGACTTAGTTGAAAAGCATGGTATTGCAATAGTTGGACAAAATCAAAAACTTGTGCCTGCAGATAAAAAAATCTATGCATTACGTGATGTAACTGGCACCGTTCAATCAATTCCATTGATTGCCTCATCAATTATGTCTAAAAAACTTGCAACTGGTTCAAACTGCATTTTATTGGATGTTAAATGTGGCAATGGTGCATTTATGAAAGATATTAATGAAGCAAAAAAACTTGGAAAATTGATGATTGAAATCGGTAAGAAATTAAATAGAAAGATTGCCGTTGAAATAACCAATATGCAACAACCTTTAGGAAAAACTATTGGAAATAAAATTGAAGTTTTGGAAGCAATAGATACATTAAATGGACACGGACCTAAAGATTTTACAGAAATTATTTACTCATCAGGTTCAACATTATTAGTTTTGGCACAAAAAGCTAAAGATGAAGTTGAAGCAAAAAAAATGATTGATGAAGTAATCAATAATAAAAAAGCATATAACAAATTTTTGGAATGA", "aa": "MSGRGLGHTGGTLDKLESIDGYNFSVTDQQFRDLVEKHGIAIVGQNQKLVPADKKIYALRDVTGTVQSIPLIASSIMSKKLATGSNCILLDVKCGNGAFMKDINEAKKLGKLMIEIGKKLNRKIAVEITNMQQPLGKTIGNKIEVLEAIDTLNGHGPKDFTEIIYSSGSTLLVLAQKAKDEVEAKKMIDEVINNKKAYNKFLE", "aa_hexdigest": "460e140dba3704043d8a288f5b54eecb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A386RHI4", "query_cov": 0.8275862068965517, "subject_cov": 0.8527918781725888, "identity": 0.53, "score": 182.0, "evalue": 3.36e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A386RHI4", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A386RHI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "LMJBGEANGM_774", "locus": "GCA013368015_00763" }, { "type": "cds", "sequence": "contig_1", "start": 432912, "stop": 433124, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAGAATTATTGATATTATTAACAAAAAAGTAGAAAATCAAACTCTTACCAAAGAAGAAATTGATTTTTTCATTAAAGAATATGTAGCAGAAAATGTTCCTGACTACCAAGCTGCTGCTTTATTAATGGCAATTAGATTAAATGGTTTAAGCGATGCTGAAACAGCACAATTAACCGACGCTATGATGCACTCAGGCGAAGTACTTGATTGA", "aa": "MRIIDIINKKVENQTLTKEEIDFFIKEYVAENVPDYQAAALLMAIRLNGLSDAETAQLTDAMMHSGEVLD", "aa_hexdigest": "36b15e58d0f699d7be26cfe5892d5407", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 62, "aa_cov": 0.8857142857142857, "hmm_cov": 1.0, "evalue": 2.7635159259117875e-25, "score": 83.47432708740234, "start": 5, "stop": 66 } ], "seq_stats": { "molecular_weight": 7899.955200000003, "isoelectric_point": 4.414707374572754 }, "id": "LMJBGEANGM_775", "locus": "GCA013368015_00764" }, { "type": "cds", "sequence": "contig_1", "start": 433141, "stop": 433857, "strand": "-", "frame": 3, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_020002487.1", "SO:0001217", "UniParc:UPI00035C52D1", "UniRef:UniRef100_A0A6A8Q0U0", "UniRef:UniRef50_A0A449A549", "UniRef:UniRef90_D1J8C3" ], "nt": "ATGACACCTCATATAAATGCAAAAGATGATGATTTTGCAAAATTAGTTTTAATGCCAGGTGATCCTTTAAGAGCAAAGTATATTGCTGAACACTATTTAGAAAACGCTAAATTAGTGTCAACAGTTAGAAATGTTTTAATGTATACTGGCTTTTATAAAGGAAGAAAAATTTCTATTTGTGCTTCAGGCATGGGCGTTCCTTCAATTGGAATTTATTCATATGAATTATTTAGCCAATATGGAGTTGAAGCAATAATTAGAATTGGATCAGGTGGCTCATTCAAGGCTGATTTAAAAAATTATTCATTAGTTTTGGCAACTTCAGCATATAGTGATAGCACTTCATTCCGTAAAAATTTATTAGTCAATGATGAAAGTAATGTTGCGCTTCCAAATAGCGAATTAAATGATTTAATTAAAAAACATGCTAAGGAATTGAATTTAAAACTTGAAGAAGGAAGAATTTTATCGGAAGATGCCTTTTATTCAGTATTTTCTGTTGAAGATAGAGTTAAAAATTCAGGCGGAGCAGTTGCTGTTGAAATGGAAGCATATGGGTTATTTACAGTAGCAGAAAAATTGCACAAAAAAGCTGCAACTATTTTAACTATTTCTGATAATTTAATAACACATGAATATACAACTGCTGAAGAAAGACAAAATTCATTCAATCAAATGATGGAATTAGCATTATCAATATCAGAAGACTTTGAATAA", "aa": "MTPHINAKDDDFAKLVLMPGDPLRAKYIAEHYLENAKLVSTVRNVLMYTGFYKGRKISICASGMGVPSIGIYSYELFSQYGVEAIIRIGSGGSFKADLKNYSLVLATSAYSDSTSFRKNLLVNDESNVALPNSELNDLIKKHAKELNLKLEEGRILSEDAFYSVFSVEDRVKNSGGAVAVEMEAYGLFTVAEKLHKKAATILTISDNLITHEYTTAEERQNSFNQMMELALSISEDFE", "aa_hexdigest": "01e48862952be26218e13d3f83512d35", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00035C52D1", "ncbi_nrp_id": "WP_020002487.1", "uniref100_id": "UniRef100_A0A6A8Q0U0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00035C52D1", "RefSeq:WP_020002487.1", "UniRef:UniRef100_A0A6A8Q0U0" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q0U0", "uniref90_id": "UniRef90_D1J8C3" }, "psc": { "uniref90_id": "UniRef90_D1J8C3", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449A549", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A549", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A549" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "LMJBGEANGM_776", "locus": "GCA013368015_00765" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 433998, "stop": 434288, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 207.5, "evalue": 2.8e-22, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "TTGCATGGCCAACTGCTACTACAAAGTAGAGGAAAATCCGCTCTAGCACTCGCTGTGATGCGAGTAGTGTTCATGCTAGACCCAATAAGTCTAGGCAACAATTTAGTTGACGACTAGTGCAACAGAGACGAGCTTATTTAGTTAAGATGCAACGATGTAAACTCCATGAGCTAGAAACCCAAATTTTGGTAGGGGAACTGGGAACAAGGAACCGAACAAAGAACCCGGAAGCCAATTGGCTTAGATAAATAGTTGGCGCTATTAAAATAGTACAGAAAGCGGTTTATAGTG", "id": "LMJBGEANGM_41", "locus": "GCA013368015_00766" }, { "type": "cds", "sequence": "contig_1", "start": 434355, "stop": 434909, "strand": "+", "frame": 3, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "COG:COG1381", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_D1J8C4", "UniRef:UniRef90_D1J8C4" ], "nt": "ATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATGTTAATGAATTTGATGAAATAATATCATTACTAACAAATAAAGGCATTCAAAGAATTTTTTGCCCCGGGGTAAATAAAACTAATTCAAAAAATGGCCCCTCAATTTTAATTTTAAACTTAGTTAATTGCGAAGTCATTAAATCAGAAACAAGAAATTTATTGCCTAGACTAAAAACTGCTTCGTTAATAAAAAATTTTCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAATTGTCTTGACAATTGCAATAATATTAATCTTATTAAAGAATATGGCCATTGCTTAGATTTTATATTGTCAAAATCTGATAAAGTTGAAGCATATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCTAACTTAAATTCTTGTATTGAATGTAACAATGTTGAAAATATTGTAGATTTTAAGCTATACAAAGGGGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCGTTATTGAGGTCTTGA", "aa": "MEFELSGIVINKQNVNEFDEIISLLTNKGIQRIFCPGVNKTNSKNGPSILILNLVNCEVIKSETRNLLPRLKTASLIKNFPTDYRLLNIANDLKTFFNCLDNCNNINLIKEYGHCLDFILSKSDKVEAYLLFLVVSSLGFKPNLNSCIECNNVENIVDFKLYKGGFLCSMHSNIVNADVSLLRS", "aa_hexdigest": "01cf238a9fe26369fdd5e454eaf0676f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8C4", "query_cov": 1.0, "subject_cov": 0.8214285714285714, "identity": 0.9890000000000001, "score": 364.0, "evalue": 9.22e-127, "valid": true, "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_D1J8C4", "cog_id": "COG1381", "cog_category": "L", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C4" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" }, "genes": [ "recO" ], "id": "LMJBGEANGM_777", "locus": "GCA013368015_00767" }, { "type": "cds", "sequence": "contig_1", "start": 435013, "stop": 435327, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "GTGGATAAAAATGAACATATTTTTAGTCAATATATTGAAGATAAACAAGACAAAGAAACATTGGCTAAAATTTCTGATGATTTTAAAAATCTGTTTGAATATTTAGAAGATAATTTTAAGCAATACTTACTATTTTATAATGAAGCATTTAATTTAATTATTAATTTACATAAAAAGAATATATCAAAGGAAGATTTAATAAAATTAAATGATTTTGAAGACATGTTACGTTCTAATAAAACAAATAACATTGATTTTAAAACATTGCAACGTAAGTTTGATGGAATAAAAAAAATATCTATGAACGATAAATAA", "aa": "MDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEDMLRSNKTNNIDFKTLQRKFDGIKKISMNDK", "aa_hexdigest": "9a73a26d98da7d984a1e06b97de335bf", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 435013, "stop": 435627, "observations": { "mutation": [ 435387 ], "stop": [ 435387 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.4928909952606635, "identity": 0.9620000000000001, "score": 192.0, "evalue": 1.28e-60, "gene_start": 1, "gene_end": 104, "reference_start": 108, "reference_end": 211, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9705882352941176, "score": 395.0, "evalue": 8.55e-143, "description": "Internal stop codon at 435387. Nonsense mutation around 435387.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12636.190799999977, "isoelectric_point": 5.684660911560059 }, "id": "LMJBGEANGM_778", "locus": "GCA013368015_00768" }, { "type": "cds", "sequence": "contig_1", "start": 435385, "stop": 435648, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "ATGAAATTATCAAAAAATCAAGAAAAAGTTCAAAATAAAAAACCACATAGATTTTTAACTAGAAGATCAAAAATAGGCATCATTTCAGGAATTAGTGCAGTTATTGGATTGCCTTTTTTACTAGGTATTACTTATGTTCTTGTTAATAATCATAAAAAAACTAATTCAAACAGTCAATTAATAATAATTCGTAAAGAAATTTATAAGCAATATTTACGTTCATTAAATTTAATTCATTCATTGCCTGATTATGGTGTATTTTGA", "aa": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPDYGVF", "aa_hexdigest": "d58776594fbb4d29ed8d9cd2697befdc", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 435082, "stop": 435648, "observations": { "mutation": [ 435387 ], "stop": [ 435387 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.41232227488151657, "identity": 0.977, "score": 169.0, "evalue": 1.66e-51, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9787234042553191, "score": 367.0, "evalue": 5.77e-132, "description": "Internal stop codon at 435387. Nonsense mutation around 435387.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10034.777599999992, "isoelectric_point": 10.53440799713135 }, "id": "LMJBGEANGM_779", "locus": "GCA013368015_00769" }, { "type": "cds", "sequence": "contig_1", "start": 435706, "stop": 435885, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCTCAATTAAAGCAACTAATAATTGATAGATTAGCCCCATTTTTTTATAAAAATAAACGTAGAGATGTTGTTATTATTCCTACAATTTTGTTTACAAATCATACTACTGCAAGTATTGAAGAAGATCCAAACCTTGATGAAAATGTTATATTATTGGCAAAATCTACTAAAAAATAA", "aa": "MPQLKQLIIDRLAPFFYKNKRRDVVIIPTILFTNHTTASIEEDPNLDENVILLAKSTKK", "aa_hexdigest": "487e9ca3fe41d15f57e66c0146051a14", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6851.9645, "isoelectric_point": 9.222669792175292 }, "id": "LMJBGEANGM_780", "locus": "GCA013368015_00770" }, { "type": "cds", "sequence": "contig_1", "start": 435889, "stop": 436299, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17770.6" ], "nt": "ATGCCTTATCATGGTGAATATAGAATGAGCGTTGTTCACGGCTATACTGCTATTGAAAGCGGTGTTGATGCTAAAAATATTTTAATTGCTAGATTAGGGGATGTTTATTATTTAGAAAATCATCAAGTTCGTTTGTCAAACGAAAAGGTTTATTTCGGGCCTGTTTTTATTGATGGCAATATTTTATCAAAAACTAACTCTCAAATTATTAAAGAACGTAGTGAATTAGGCCAAAATGGATTTATGCATGTTGTTTTAGCAATTAATAAAGAAAAGAATCTAATTATTGGTAAACCAAGAATAGTTTCTCGTGGTGCATTTTATGTAAAAAATTCATTGCATTTAATTGAAGAAGCTAAGAGATTGGTTCATGGCGCTATATTATATACAATTAAAAACTCAAATGATTGA", "aa": "MPYHGEYRMSVVHGYTAIESGVDAKNILIARLGDVYYLENHQVRLSNEKVYFGPVFIDGNILSKTNSQIIKERSELGQNGFMHVVLAINKEKNLIIGKPRIVSRGAFYVKNSLHLIEEAKRLVHGAILYTIKNSND", "aa_hexdigest": "20886ae1a00e5afdf394dd569c4a12bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17770.6", "name": "RNase_J_C", "length": 60, "aa_cov": 0.4411764705882353, "hmm_cov": 1.0, "evalue": 7.5431038716899e-14, "score": 48.14616012573242, "start": 69, "stop": 128 } ], "seq_stats": { "molecular_weight": 15400.56679999999, "isoelectric_point": 9.300031852722167 }, "id": "LMJBGEANGM_781", "locus": "GCA013368015_00771" }, { "type": "cds", "sequence": "contig_1", "start": 436522, "stop": 436983, "strand": "-", "frame": 3, "gene": null, "product": "ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6", "UniRef:UniRef90_UPI003080D5B6" ], "nt": "ATGATGACAGGTGATTTTAGGTTTGACTACACACCTATTGGAAATTTTACAGATTTTAATAAACTTAAACAAATTGGCGACGAAGGCCTTGATGTTTTATTTTCTGATTCAACAAATGCTATGCGTCCAAATCATTCACCATCAGAGTCAGATATTTTAAAAGATATTAAAAAATATATGTTGGAAGCGGAAAAGAAAATCATTGTAACTGCATTCGCTTCTAATTTGACTAGAATTAAAGCAATTATTGAATTAGGTGCTTCTTTAAATAAAAAGGTTGTTGCCTTCGGCCGTTCTATGGTTGATGGAATAGATATTGGAAGAAGACTAGGTTATATTAATGTTCCAGACGAAGTGTTTATTGACAAAAAATCATTAAGTAAATTTGGAGATAATGAATTATTAATTTTAACAACTGGTTCTCAAGGTGAAGAACTAGCTGGACTTGCAAAGATGTCGTGA", "aa": "MMTGDFRFDYTPIGNFTDFNKLKQIGDEGLDVLFSDSTNAMRPNHSPSESDILKDIKKYMLEAEKKIIVTAFASNLTRIKAIIELGASLNKKVVAFGRSMVDGIDIGRRLGYINVPDEVFIDKKSLSKFGDNELLILTTGSQGEELAGLAKMS", "aa_hexdigest": "e42de43bff8d4d1683c236ec3a4bfa47", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 436567, "stop": 437115, "observations": { "mutation": [ 437049, 437079 ], "stop": [ 437049, 437079 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003080D5B6", "query_cov": 0.9019607843137255, "subject_cov": 0.7540983606557377, "identity": 0.855, "score": 233.0, "evalue": 2.82e-76, "gene_start": 1, "gene_end": 138, "reference_start": 46, "reference_end": 183, "reference_sequence": "TNVEFIEINKDLVVKFGECSDDWWTAQHSIPDAFGVRVKTPTGSLMLTGDIRFDYTPIGNLTDLSKLMKIGEEGLDVLFSDSTNAMRPNHSPSASDILKDIKKYMLEAEKKSIVTAFASKLTRIKAIIELGASLGKKVIAFGRSMIYNIDIGRRLGYINAPDEVFIDKKHLSKYNDNELLILT" }, "paralog": false, "identity": 0.8131868131868132, "score": 295.0, "evalue": 4.46e-103, "description": "Internal stop codon at 437049, 437079. Nonsense mutation around 437049, 437079.", "psc": { "uniref90_id": "UniRef90_UPI003080D5B6", "product": "ribonuclease J", "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003080D5B6", "UniRef:UniRef50_UPI003080D5B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6" ], "product": "ribonuclease J" } }, "id": "LMJBGEANGM_782", "locus": "GCA013368015_00772" }, { "type": "cds", "sequence": "contig_1", "start": 437077, "stop": 437448, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33" ], "nt": "ATGAGCCCAACTAAAATTTTTGGATTAGGCGGAATGCAAGAAATTGGTAAAAGTACTTTGGTTATTCAATATGAAAATCATATTTTTATTATTGATACCGGAATTAAATTTTGCGATTCATATGTAACAGGTGTAAATGGTTCTATTCCAGATTTCACTTTTTTAGCAGAAAATCAAGATAAAATCGAAGGTCTTTTCATAACCCACGGACATGAAGATCATATTGGAGGTGTTCCATATTTAGTTCAACAAGTTGATGTTAAAAAAATATTTGCACCTAGAATTGCTATTCAATATCTAAAACTAAAATTTGAGGATATGAAGATAAAACGTAATATTGAATTTATTGAAACTGAAAAAGATGCAGTTTGA", "aa": "MSPTKIFGLGGMQEIGKSTLVIQYENHIFIIDTGIKFCDSYVTGVNGSIPDFTFLAENQDKIEGLFITHGHEDHIGGVPYLVQQVDVKKIFAPRIAIQYLKLKFEDMKIKRNIEFIETEKDAV", "aa_hexdigest": "5657dcf5c352ec4d827c4c65c5ef9a20", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 77, "aa_cov": 0.6260162601626016, "hmm_cov": 0.9090909090909091, "evalue": 5.7902427896172336e-12, "score": 41.480987548828125, "start": 18, "stop": 94 } ], "seq_stats": { "molecular_weight": 13935.941499999986, "isoelectric_point": 5.498797416687012 }, "id": "LMJBGEANGM_783", "locus": "GCA013368015_00773" }, { "type": "cds", "sequence": "contig_1", "start": 437567, "stop": 437689, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATAGTAGGGTACAAAAAACAAAGATTCCTAAAGTATTAGCAAGTTTTAGTTTAATTTTAGAACTTATTGCATTAGCATTACTTATTTATGTTATATATAGTTTCTCTTCAATTTGA", "aa": "MNDSRVQKTKIPKVLASFSLILELIALALLIYVIYSFSSI", "aa_hexdigest": "13087fb91022485fd00f91ad0075f999", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4499.399499999999, "isoelectric_point": 9.398732948303223 }, "id": "LMJBGEANGM_784", "locus": "GCA013368015_00774" }, { "type": "cds", "sequence": "contig_1", "start": 437861, "stop": 438058, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGTAATAGCAAAAGCTAGTTGGTCATTACACAAAGTTCCAATAATACTTATTATTGTTACATTATGTTTGCCAATTCTATTATTATGTGAAATAGTTAGAATAGTATCACTATTTTTAATAGTAATAAAAAAGCAAAAAAATTTTAATAACCAAGTATATTATGGACCACAAGTTGCTAACATACAAAATTAA", "aa": "MLVIAKASWSLHKVPIILIIVTLCLPILLLCEIVRIVSLFLIVIKKQKNFNNQVYYGPQVANIQN", "aa_hexdigest": "14e247eede5c1be5a89f9c0c5c92f27e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7402.055300000002, "isoelectric_point": 9.698317527770996 }, "id": "LMJBGEANGM_785", "locus": "GCA013368015_00775" }, { "type": "cds", "sequence": "contig_1", "start": 438124, "stop": 439059, "strand": "-", "frame": 3, "gene": "trxB", "product": "Thioredoxin reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "RefSeq:WP_175936756.1", "SO:0001217", "UniParc:UPI001589ECB7", "UniRef:UniRef100_UPI001589ECB7", "UniRef:UniRef50_A0A448ZW45", "UniRef:UniRef90_D1J8C8" ], "nt": "ATGGAAAATAATAACTTATATGATGTTTTAATAATTGGAGCAGGACCGGCAGGATTAACAGCTGCAATATATTTAGCAAGAAATGACATAAATGTTGCTTTTATTGAAGGCGGAGTACCAGGAGGAAAAGTTTCTGCTCAATCTAAAATTGAAAATTATCCAGGTTTTAGTTTTATAAGTGGTTTTGAACTTTCTAAAACCATGCTTAAACAAGCAAGAGATAATGGTTCAAAATTTATTTACGGTAATGTAAAGGAAATTAATTCTATATCAGATATTGAAAAAGAAGTCATTTTGGAAAATGGCATGTCAATAAAATCAAAAGCAATAATTATTGCAACCGGCATGAAAGAAATTGTTCCCTTGGACGTAAAAGGCATTGAAGAATATAGAGGCAAAGGCGTATCATATTGCGTAATTTGCGATGGTCCTTTGTATAAAAATAAACCTTGTGCAATCATTGGTGGAGGTAATTCAGCATTCGAAGAATCGTCTTATTTAGCATCAATTGCCTCTGAGGTTCATATTTTTGTTCGTGATGGAATTATTGCAGAAAAAAAACTTGTCAATGAAGCAATGTCTAAAAGCAATATTTATATTCATGAAAATTCAACTATTAAAGAAATTTTGGGCAATGAAAAAGTTGAAGAAATTATTGCTAATGTAAATGGAAAAGAAACAAAAATGAATATAAGTTGCATTTTTCCTTATGTTGGTTTTAAACCTAACACAGCATTTATAAAAGGTAAATATGATGAAATATTAAATAGCCGTGGATTTATTGAAGTTAATGAAAATATGGAAACTAAAATTCCTAATATTTTTGCAATAGGCGATGTTATTGTAAAAGATGTAAGACAAATTACAACAGCAACAGCTGATGGGACAATTGCTGCAAGAGCAATAAGCAATAAAATCGATAAATTATATAAATAA", "aa": "MENNNLYDVLIIGAGPAGLTAAIYLARNDINVAFIEGGVPGGKVSAQSKIENYPGFSFISGFELSKTMLKQARDNGSKFIYGNVKEINSISDIEKEVILENGMSIKSKAIIIATGMKEIVPLDVKGIEEYRGKGVSYCVICDGPLYKNKPCAIIGGGNSAFEESSYLASIASEVHIFVRDGIIAEKKLVNEAMSKSNIYIHENSTIKEILGNEKVEEIIANVNGKETKMNISCIFPYVGFKPNTAFIKGKYDEILNSRGFIEVNENMETKIPNIFAIGDVIVKDVRQITTATADGTIAARAISNKIDKLYK", "aa_hexdigest": "91fa24ca41252333e9818e5acd00d566", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI001589ECB7", "ncbi_nrp_id": "WP_175936756.1", "uniref100_id": "UniRef100_UPI001589ECB7", "db_xrefs": [ "SO:0001217", "UniParc:UPI001589ECB7", "RefSeq:WP_175936756.1", "UniRef:UniRef100_UPI001589ECB7" ] }, "ips": { "uniref100_id": "UniRef100_UPI001589ECB7", "uniref90_id": "UniRef90_D1J8C8" }, "psc": { "uniref90_id": "UniRef90_D1J8C8", "gene": "TrxB", "product": "Thioredoxin reductase", "uniref50_id": "UniRef50_A0A448ZW45", "cog_id": "COG0492", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW45" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "LMJBGEANGM_786", "locus": "GCA013368015_00776" }, { "type": "cds", "sequence": "contig_1", "start": 439094, "stop": 439351, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCTTTACGTGATGGCAGTTATACTATTTATAAAGTAATTTCTGCTTTATATATAATAGTTGGTTTGGTATTGACTGTAATGTTTGAATTCGTTATAAAATTGAATTACAATGTTTATAAAGTTAATACATATAATAACGAAAAGCTTTCAAAATTCTTTTACTTTATAGTTTATGAACCTAAAAATTCAAACTCATTTGCATTTAAAAAATCTACTACTAAAAATCAAATAGAAACTAAAACAGCAGCTTAA", "aa": "MEPLRDGSYTIYKVISALYIIVGLVLTVMFEFVIKLNYNVYKVNTYNNEKLSKFFYFIVYEPKNSNSFAFKKSTTKNQIETKTAA", "aa_hexdigest": "8a0fbf5055710196ae29c000861a2e52", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9942.458099999989, "isoelectric_point": 9.456045341491702 }, "id": "LMJBGEANGM_787", "locus": "GCA013368015_00777" }, { "type": "cds", "sequence": "contig_1", "start": 439433, "stop": 439669, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAAGCAATTACTATAATTTTACCAGCGGTATTAATTGGACAAGCAATTGGACGTTGGGGAAACTTCGCAAACCATGAAGTTTATGGTGGCGTTATGGAAAAAGGCTCTGTTGCATTTGAAATATTGCCAAAATTAATTAGGGATCACATGTTTATTGATGGAAAATACCGTTATCCTTTATTCTTCTATGAATCAATTGCAAACTTAGCTGCTTATATTATCTTAGTTTGA", "aa": "MKKAITIILPAVLIGQAIGRWGNFANHEVYGGVMEKGSVAFEILPKLIRDHMFIDGKYRYPLFFYESIANLAAYIILV", "aa_hexdigest": "14942c0b7e224d900c4c469552ea11dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8817.391399999997, "isoelectric_point": 9.049249839782714 }, "id": "LMJBGEANGM_788", "locus": "GCA013368015_00778" }, { "type": "cds", "sequence": "contig_1", "start": 440114, "stop": 442330, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "TTGTTTATTGATAGAGTTAAATTATCAAATGATAATGATATAAAATCAAGAATATCAAGTGCAATTGAGGTTGCATTGGAGCAATCTAATGGTTTAATTTCAACTATTGTTAATGAAAGCAAAAAGAATACCTATTCAATACATCATAGTTGCAGTTTTGGCGACTTTGATATGCCAAAAATTGAACCTAAATTATTCTCGTTTAATTCTCCAGCTGGAATGTGTCCTGAATGCAAAGGACTTGGCGTTATTCAAAAGGCAAGCTTTGATTTAATTTGTCCCGATAGGCAGCTTTCTATAAACGAAGGAGCAATAAAGTATTATAGAAATTTCATGAATACTTACAATTTAGAATGGCAAGAATTTGACAAGCTATTAAATTATTATCATATTGATAAAGATCAACCTATTGATTCTTTATCTTCTTTTGAACTAGATATTATTCAACATGGGTCGCAAGAAGAAATATCATATGTGTTATTATCCGAAAGTGGCACAAAACGCGAGAAGTTTCAAGTTATTGAAGGTATTGTTGATAAATTACAAAGGAAGTATTTTGATACAAAATCACTAGAAGCTAGGGCTTATTATAGCAAATACATTGGAGACATTACTTGCCCTTCATGTCATGGAAAGCGTTTGAATAAATATGCATTGTCTTGCAAAATTCTAGATCTAGATATATACGAAATTTGCAATAAGTCGCTTGGAGATTTAGCAATTATTTTAGAAAGATTGCAGTTAAGTGATTTAGAAAAAGAAATTACCTCTTTAATTTTGTCTGAATTAAAACATAGAATTAAATTTTTAATTGATGTTGGCTTAAAGTATTTAACATTGAATAGAAAAGCTGAGACATTAAGCGGTGGTGAAGCTCAAAGAATTAGATTAGCAACACAAATTGGTTCTAATCTAACAGGCATTTTATATGTTTTAGATGAACCTTCAATTGGCTTACATCAAAAAGATAATGAAAAATTGCTAAGCTCTTTAAGAAAAATGGTTGAATTAGGCAATAGTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGACAAGCAGATTATATAGTTGATATTGGTGTTCAAGCAGGCGAACATGGTGGAAATATTGTTGCATGTGGAAATGTTGAAAATATAATTGCATGTAAGCAAAGTCTAACTGGCCAATATTTGTCAAAAGAAAGACAAATAGAGGTGCCAAAATTTAGGCGGAGTGGAAATGGCAAGACATTAACATTAAAAAATGCAAAATTAAATAATTTAAAAAATATTAAAGTAACTTTTCCATTAGGAAAATTTATTGCAATTACTGGAGTTAGTGGTTCTGGAAAATCTACATTAATAAACGATGAACTAGTTGGAAGTCTTAATAAATATTTATTAGATCAATATTATGATATTCAAAGAGATAGTAAATTAATTGGTCAAACTAACATTGATAAATTAATTCAAATTGACCAAAATCCAATCGGGCGTACTCCTAGATCAAATCCTGCAACATATACTGGTGTTTTTGACGATATTAGAGAAATTTTTGCAAATACCGAAGAAAGTAAAATTAGAGGATATACAAAATCAAGATTTTCATTTAATGTTCCAGGTGGGCGTTGTGATAAATGCGAAGGCGATGGTGTAATCAAAATTGCTATGCATTTTTTGCCCGATGTTTATGTGATATGCGACCATTGCAACGGGAAACGTTATAATCAAGAAACGCTAGAAATAAAATATCATGGTAAAAGTATTGGCGATGTTTTGGATATGACAATTGAAAAAGCCTATGCATTATTTATAAACAAGAGCCAAATTAAAGAAAAATTGCAAGTTTTATTAGATGTTGGACTTGGCTATATTAAATTAGGCCAAAATGCTACGACATTAAGCGGTGGTGAAGCTCAAAGGGTAAAATTAGCAACGTACCTACAAAAAAAGCCAACAGGCAAATCTTTATATGTATTAGATGAACCAACAACCGGATTACATCCTTACGATATCTCTAATTTATTAATAGTATTAAATAGAATAGTAGACAATGGCGATACAGTAATTGTAATTGAACATAATTTAGATTTAATTAAATGCGCTGATCATATTATTGACCTAGGCCCTGATGGTGGGGATGAAGGGGGAAAGGTTGTTTGCTCTGGAACACCTGAACAGGTCTCAAAGTGTGAAAATTCGTACACGGGGCAATATTTAGCAAAACTTTTATAG", "aa": "MFIDRVKLSNDNDIKSRISSAIEVALEQSNGLISTIVNESKKNTYSIHHSCSFGDFDMPKIEPKLFSFNSPAGMCPECKGLGVIQKASFDLICPDRQLSINEGAIKYYRNFMNTYNLEWQEFDKLLNYYHIDKDQPIDSLSSFELDIIQHGSQEEISYVLLSESGTKREKFQVIEGIVDKLQRKYFDTKSLEARAYYSKYIGDITCPSCHGKRLNKYALSCKILDLDIYEICNKSLGDLAIILERLQLSDLEKEITSLILSELKHRIKFLIDVGLKYLTLNRKAETLSGGEAQRIRLATQIGSNLTGILYVLDEPSIGLHQKDNEKLLSSLRKMVELGNSLIVVEHDEETIRQADYIVDIGVQAGEHGGNIVACGNVENIIACKQSLTGQYLSKERQIEVPKFRRSGNGKTLTLKNAKLNNLKNIKVTFPLGKFIAITGVSGSGKSTLINDELVGSLNKYLLDQYYDIQRDSKLIGQTNIDKLIQIDQNPIGRTPRSNPATYTGVFDDIREIFANTEESKIRGYTKSRFSFNVPGGRCDKCEGDGVIKIAMHFLPDVYVICDHCNGKRYNQETLEIKYHGKSIGDVLDMTIEKAYALFINKSQIKEKLQVLLDVGLGYIKLGQNATTLSGGEAQRVKLATYLQKKPTGKSLYVLDEPTTGLHPYDISNLLIVLNRIVDNGDTVIVIEHNLDLIKCADHIIDLGPDGGDEGGKVVCSGTPEQVSKCENSYTGQYLAKLL", "aa_hexdigest": "c0ba65437d950be998dc20257c741a27", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P0N4", "query_cov": 0.8170731707317073, "subject_cov": 0.9934102141680395, "identity": 0.73, "score": 885.0, "evalue": 0.0, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "LMJBGEANGM_789", "locus": "GCA013368015_00779" }, { "type": "cds", "sequence": "contig_1", "start": 442340, "stop": 442942, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "ATGAACGATAAAATAATAATAAAAGGTGCTAGAGAAAATAATTTAAAAAATATTAATTTAGAAATTCCACGTAACAAATTTGTGGTTTTTACTGGTGTCAGTGGCTCTGGTAAAAGTTCTTTAGCATTTAATACAATCTATGAAGAAGGCAGACGTAGGTATGTTGATAGCCTTTCTAATTATGCAAAACAATTTTTAGGTGGTACTAAAAAACCCAATGTTGATAGCATTGAAGGATTGAGCCCAGCAATTTCTATTGAACAAAAAACAGTACATGTTAATCCTAGAAGTATAGTTGGTACTGTTACTGAAATTTATGATTATTTAAGATTGCTATATGCAAGAGTAGCAAAACCTTATTGCCCAAATCACAAAATTGAAATTACTGCTCAAAAAACAAAAGACATTATTTCTAACATTTATAAAAACCCATTAGGATCAACAATATACATATTAGCCCCATTAGTAAATAACCAAAAAGGAAGCCACCAAGTATTACTTAATAAACTAAAAAGAGATGGCTTTATAAAAGTTTTAATAAATGATGAGATTTATTTCTTAGAAAATGTTGATTCGATTAATCTAGATAAAAATAAACGTTGA", "aa": "MNDKIIIKGARENNLKNINLEIPRNKFVVFTGVSGSGKSSLAFNTIYEEGRRRYVDSLSNYAKQFLGGTKKPNVDSIEGLSPAISIEQKTVHVNPRSIVGTVTEIYDYLRLLYARVAKPYCPNHKIEITAQKTKDIISNIYKNPLGSTIYILAPLVNNQKGSHQVLLNKLKRDGFIKVLINDEIYFLENVDSINLDKNKR", "aa_hexdigest": "cad32588a4257501976ecf98a2c0e633", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_W7DIQ3", "query_cov": 0.99, "subject_cov": 0.9611650485436893, "identity": 0.5710000000000001, "score": 232.0, "evalue": 1.53e-74, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_W7DIQ3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 69, "aa_cov": 0.345, "hmm_cov": 0.9710144927536232, "evalue": 1.8890896889578323e-19, "score": 65.14056396484375, "start": 131, "stop": 199 } ], "seq_stats": { "molecular_weight": 22626.790200000025, "isoelectric_point": 9.641972160339353 }, "id": "LMJBGEANGM_790", "locus": "GCA013368015_00780" }, { "type": "cds", "sequence": "contig_1", "start": 443088, "stop": 443645, "strand": "-", "frame": 1, "gene": null, "product": "HipA-like C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8D1", "UniRef:UniRef90_D1J8D1" ], "nt": "GTGGATGGCGAAATTCATTTATATAAACGAAACTCAAAATTTGCAAATAAAAACTTATACGATGTTTATAGTGAACATTTTGCAAGTCAAGTTGCCAAATTTTTGAATTTGCCGGCGGTTGATTATAAAATTACTAAGTATGGAAAGCATTATATTAGTGATTGCAAATTATTTACAAACAAAGATTTTTCATTCATCCCATTTTCTAAAATCATAGATCCCAATATTAATGGTAATAATTTAAAATTATGTAGTCAGATTGCTTTTTATTATGGCAAAGAGTTATTTGAAGATTTAATGGTTTTTGATGCTTTAATTTGTAATGGGGATAGGCATTTAAATAATTTTGGCATGATGTTGGATAATAATAGCGGAATTTTAACAAAGCCAGCTCCAATATTTGATTTCAACAATAGTTTAATTTTTGATTATCCTATTTATTTAAAATCAATTGCAAAAACTGCATTTTTAAATGACTATGAGAATCGCAAGACCAGGTTTTTTGAAAGTTTTAATCAACAACTAAAAATTTTTATTAGAAAAAGACATTTAGATTGA", "aa": "MDGEIHLYKRNSKFANKNLYDVYSEHFASQVAKFLNLPAVDYKITKYGKHYISDCKLFTNKDFSFIPFSKIIDPNINGNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLD", "aa_hexdigest": "35a8fddbf1d94bdefe09424ea3049215", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 442932, "stop": 443945, "observations": { "mutation": [ 443090, 443654, 443684, 443795, 443831 ], "stop": [ 443090, 443654, 443684, 443795, 443831 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8D1", "query_cov": 1.0, "subject_cov": 0.4731457800511509, "identity": 0.968, "score": 374.0, "evalue": 4.77e-128, "gene_start": 1, "gene_end": 185, "reference_start": 155, "reference_end": 339, "reference_sequence": "MKTYYLKCKNKICLSFEADEKLTTLSNIKLLDDFIFIKQKYNEYSPRQWLEFILNISLIPMQRPNYKGLIENFLNYKKLILNNNYLNLCNSFWICSQEESCKLNWEDVNYFDHFNNDSCNYFLDYKAKLAETNRLQIKSPSWFIDGDMLKAWVEVDGEIHLYKRNSKFANKNLYDVYSEYFASEVAKFLNLPAVDYKITKYGKHFVSDCKLFTNKDFSFIPFSKIIDPNINGNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILIKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLDWIKQLKKFKFKQPNKIKMDMKYLKFINKFFKKRIKKAEQYLIEKGYIKENER" }, "paralog": false, "identity": 0.9614243323442137, "score": 661.0, "evalue": 8.69e-244, "description": "Internal stop codon at 443090, 443654, 443684, 443795, 443831. Nonsense mutation around 443090, 443654, 443684, 443795, 443831.", "psc": { "uniref90_id": "UniRef90_D1J8D1", "product": "HipA-like C-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J8D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8D1", "UniRef:UniRef50_D1J8D1" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8D1" ], "product": "HipA-like C-terminal domain-containing protein" } }, "id": "LMJBGEANGM_791", "locus": "GCA013368015_00781" }, { "type": "cds", "sequence": "contig_1", "start": 444115, "stop": 444273, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGAAAATTTACAAAAAATATTGCATCGTGATCTTTCAAAAGAAATTGCAGAAGTTGAAGAAAAGTATAAAAATCTAGCAAAGGAAGACCACAAGGAAGATCCAAAACCAAAATCAAAAGATAATAAAAACGATTTTGATGGCGAAATAAATTAG", "aa": "MLENLQKILHRDLSKEIAEVEEKYKNLAKEDHKEDPKPKSKDNKNDFDGEIN", "aa_hexdigest": "7f6aae06fd24ab1bee0186424e2158cd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6165.808100000002, "isoelectric_point": 5.288891029357909 }, "id": "LMJBGEANGM_792", "locus": "GCA013368015_00782" }, { "type": "cds", "sequence": "contig_1", "start": 444283, "stop": 444705, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE", "UniRef:UniRef90_UPI001F2C38EE" ], "nt": "ATGTCAATCGAAGATTATGATGGCGGGTTTGGAACTAAAGAATTTTCTCTTGAATCTGAATTATCAGAAGATGAAGAATCTGATGAAAAATCAGATGATGGCGATTCAAATGATGAAGAAATAGATATGAGCAGTATGGATGATTTTAAAAATTCATTTGTAAATTTCTTAGAAGGTTTTGATGATTTAAAACAACAAGCAAAAAAATTAAAAGAACAAATTGCTGATAATAAATTTATGTCTGGTTCTCCATTTGAAATGAATTATGAAGATCTAAGTATGGAAAAATTAGTTGATAGTATGTATAAAGCTGCCAAAGCAATTGGAAATGTTCGTGATGACGAAATGGTTGATTATTTCAAGGGTGCATTCGATGAATTAAGAGGAATGGGAATAAATAATATTCCTAATGACGGTTCATGA", "aa": "MSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNDEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGS", "aa_hexdigest": "ff5801b1df743f872f7cfd637333fe06", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 444115, "stop": 444778, "observations": { "insertion": [ 444721 ], "stop": [ 444285 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2C38EE", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 0.993, "score": 263.0, "evalue": 1.09e-87, "gene_start": 1, "gene_end": 140, "reference_start": 25, "reference_end": 164, "reference_sequence": "MKFSREFIGKQNLSNRRSIPIERRMSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNEEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGSWEKSMLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN" }, "paralog": false, "identity": 0.9181818181818182, "score": 389.0, "evalue": 1.25e-139, "description": "Internal stop codon at 444285. Frameshift due to insertion around 444721.", "psc": { "uniref90_id": "UniRef90_UPI001F2C38EE", "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2C38EE", "UniRef:UniRef50_UPI001F2C38EE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15832.989399999991, "isoelectric_point": 4.0500284194946286 }, "id": "LMJBGEANGM_793", "locus": "GCA013368015_00783" }, { "type": "cds", "sequence": "contig_1", "start": 444877, "stop": 445248, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGATTTAAACACTTTTAATTCCTTAAATATTTTTAATGATTTTTTAATAAACGACGATGAAGAAAATAAAAATTCTTTTGACTTTAAAAAATATGGACTTGAACAAAATGAAATTATTTTCTCTGCAATTTTACCTACAAAAGAAATTATTTCATTAGCATTAAAAATTGATTTTGTTTATGATGATAAAAGAGAATTTGATGAAGATTTTCAAATTGATCCTGAATTAATTAGTCAAAGTGTAATTTCTGAAAGAAAAGAAACTTTAAAATATTTAAAAGAATTAAAGCAAAATGAATTTAAAAAATTTCACACAAACCCTTCAAATGATATTAAAAATATAACTAGAAGTGATTTTAGCAAATGA", "aa": "MSDLNTFNSLNIFNDFLINDDEENKNSFDFKKYGLEQNEIIFSAILPTKEIISLALKIDFVYDDKREFDEDFQIDPELISQSVISERKETLKYLKELKQNEFKKFHTNPSNDIKNITRSDFSK", "aa_hexdigest": "2327adaa295d0b22f2aed847103bcbb6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14600.144199999979, "isoelectric_point": 4.6740636825561515 }, "id": "LMJBGEANGM_794", "locus": "GCA013368015_00784" }, { "type": "cds", "sequence": "contig_1", "start": 445327, "stop": 445536, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAGAAGCAATTAGGAATAAATCTAAAATTAACAGTTAGAGAATTAAATGTAGCTTTTGACTACGAATTTAAGCCAATAGAAGCAGTTAAAAAAATTAATTTTGAACCAATATCATTTCCAATAACTTCAGGGATTTCTTTTTCTGAATTAGTGGATGTTTTAGGCGCATTTTTTGGCTTTGAAAAGAGTGTTGTATTTCATTGA", "aa": "MIKKQLGINLKLTVRELNVAFDYEFKPIEAVKKINFEPISFPITSGISFSELVDVLGAFFGFEKSVVFH", "aa_hexdigest": "8369d173a9642d4d732bab994d7713c5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7838.120199999998, "isoelectric_point": 6.552023887634278 }, "id": "LMJBGEANGM_795", "locus": "GCA013368015_00785" }, { "type": "cds", "sequence": "contig_1", "start": 445529, "stop": 445810, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTAGAAAATGTTTATAAGAATGTAGAAACATCTAAAGATGAAATAATGACAATCGGGGATGGAAATAATGATATACCAATGTTGTCAATATCAAAATATTCGTATGCAATGGCAAATGCAACACAAACTGTACATGAACATAGCCACTTTCATACAAGTGCTGTTGAACAAAATGGTTTGGGAGAAGCAATACTTGATTACATATATAGATTAAAAAATATAACTAAAAAATATATGTTGCATGAATTTGAAAACAATAGAGGAGTAGAAAATGATTAA", "aa": "MLENVYKNVETSKDEIMTIGDGNNDIPMLSISKYSYAMANATQTVHEHSHFHTSAVEQNGLGEAILDYIYRLKNITKKYMLHEFENNRGVEND", "aa_hexdigest": "70081469e4d8032eb2acaec8be293d33", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 64, "aa_cov": 0.6881720430107527, "hmm_cov": 1.0, "evalue": 1.5112334711187805e-18, "score": 62.95213317871094, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 10682.783399999993, "isoelectric_point": 5.228414344787596 }, "id": "LMJBGEANGM_796", "locus": "GCA013368015_00786" }, { "type": "cds", "sequence": "contig_1", "start": 446174, "stop": 446449, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGTCAAACAATTTTAAAAGTAACCAACAACAAGAAGCCAATTGCTTTTTCAGATGTTGATGGTACCATATATAAAAATTTTAATTTAAAGCAAGAAACAATCGACGATATCCATTTTGCAGTAGACAATGGGTTAGATTTTAATATATGTACTGGAAATCCAATTCAAGAAAGAATGTTGAAACTAGCCGAAACATTAAAAGCAAGATATTTAATAGGATCTTCTGGCGCTCAAATTTTTGATATGCAGTCAAGAGAATATATTGCCTGTTGA", "aa": "MSQTILKVTNNKKPIAFSDVDGTIYKNFNLKQETIDDIHFAVDNGLDFNICTGNPIQERMLKLAETLKARYLIGSSGAQIFDMQSREYIAC", "aa_hexdigest": "3d09afb544d3af4ccad0b0724e2ecdca", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 75, "aa_cov": 0.8021978021978022, "hmm_cov": 0.9733333333333334, "evalue": 3.7429365930706917e-10, "score": 35.44811248779297, "start": 17, "stop": 89 } ], "seq_stats": { "molecular_weight": 10276.648599999993, "isoelectric_point": 5.686195564270019 }, "id": "LMJBGEANGM_797", "locus": "GCA013368015_00787" }, { "type": "cds", "sequence": "contig_1", "start": 446466, "stop": 447269, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175936761.1", "SO:0001217", "UniParc:UPI00158EE4AC", "UniRef:UniRef100_UPI00158EE4AC", "UniRef:UniRef50_A0A454CA04", "UniRef:UniRef90_A0A454CA04" ], "nt": "ATGAATAAAAAACAAATAAAAAAATTTTTAAGTTTTTCATTGGCTATTCCGACTTTACTAACTATCCCATTAGTTTCATTATCGTGCAAAAAATCAAAAGATAATCAAGATAATTTAGAATTAGGCAATGATGACGGAATTGAAAAACAAGATTTAAGTGTTGCCAAAAGAATAGTTGAAATATTTAAAGAACACAGATTTTCATTTGATGAAATGAATCAAAAATTATTCTTGCAAAATCTAAATATAGAAGACCCTAAAAAACCATTTAAAAAAATGAGATTTGAAGATTTTTATAACAATAGAGTAAAAGATTGGAATTTTAAAGAAAAGTCTGAATACAATAGAGCTTTGGAGTTGGATAATGATCTTAAATATCCCTTCTTTGATATAAAGAATCAAAATAGTTTTATTAATTTATTAAAAAATGATCCTGATCCTAATCTTTATAGGGTTTTTAATAAATATTTTCAAATAAGATTAAGAACAAATGACGAACTTTTAAAAATACTTTTTGTGTTTGAATTGGTGTCAAAAAAAATGCAACAACAATTAAGCAATGAAATAAAGAAGGATGCCTCGGATTTAAATGTAAAGAATTATTTCAATTTAAAAACATATGCCATAAATCATTTTTTATTAGATTATACGGAATTTCTAAAATCAGAAGAACCTGAAACCACAATGCATTATACATTTAGACTATTAAGCTATATTGTGCCACCAGTTGCCATTTCTGGCATTATAATTTATATTATTATAATGGTATTAAAAAAGAAACATAAATCAAAGATTAGAGCATAA", "aa": "MNKKQIKKFLSFSLAIPTLLTIPLVSLSCKKSKDNQDNLELGNDDGIEKQDLSVAKRIVEIFKEHRFSFDEMNQKLFLQNLNIEDPKKPFKKMRFEDFYNNRVKDWNFKEKSEYNRALELDNDLKYPFFDIKNQNSFINLLKNDPDPNLYRVFNKYFQIRLRTNDELLKILFVFELVSKKMQQQLSNEIKKDASDLNVKNYFNLKTYAINHFLLDYTEFLKSEEPETTMHYTFRLLSYIVPPVAISGIIIYIIIMVLKKKHKSKIRA", "aa_hexdigest": "ec2b38bf657d640c043214b0067cc5c3", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158EE4AC", "ncbi_nrp_id": "WP_175936761.1", "uniref100_id": "UniRef100_UPI00158EE4AC", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EE4AC", "RefSeq:WP_175936761.1", "UniRef:UniRef100_UPI00158EE4AC" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EE4AC", "uniref90_id": "UniRef90_A0A454CA04" }, "psc": { "uniref90_id": "UniRef90_A0A454CA04", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA04" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA04", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA04" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_798", "locus": "GCA013368015_00788" }, { "type": "cds", "sequence": "contig_1", "start": 447595, "stop": 448227, "strand": "+", "frame": 1, "gene": null, "product": "Vaa surface lipoprotein adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103", "UniRef:UniRef90_O53103" ], "nt": "ATGAAAAAATCAAAGAAAATATTTATAACATTATGTGGAATAGCAACTACTGCTATTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCTTTAAAACAAGCAAACACATTAGCAGAAGAATTGAAAAAAAATCCTGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCCGAAGCAACCAAATCATTTAAAGAAGCAGGTTCATACAGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAATAGAAAATGCAAAAAGTGAAAAGAAAACAATTGACGACAAGAATGCTCAAATTGCAAAAGAATTAGCAGAAAGAAATGCTAAAATTCAAAGTAATATTGAAGAACTTAAAAAAATCAATAATGAAGCTTTTGAACTTTCAAAAACTGTAAACAAAACAATTGCTGAAGTAGAAAAGAAATTTAAAATAGAAGCATCATTCAAAGAACAACTAGAAAATTTTGCAGATGATTTATTAGACAAAAGCCGTCAAATTGATGAATTCACCACAGTTACTTCAGCTCAAGAAGGTTTTACTCTAGCAGAACTAGAATCATTTAAAGAAATAACCACTACTTGA", "aa": "MKKSKKIFITLCGIATTAILPVATISCNDDKLAEKNGKEKADAALKQANTLAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYSDYPAIISKLSAAIENAKSEKKTIDDKNAQIAKELAERNAKIQSNIEELKKINNEAFELSKTVNKTIAEVEKKFKIEASFKEQLENFADDLLDKSRQIDEFTTVTSAQEGFTLAELESFKEITTT", "aa_hexdigest": "45b088a6a03dab3ad886fb6c25fb7091", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_O53103", "query_cov": 1.0, "subject_cov": 0.8898305084745762, "identity": 0.9520000000000001, "score": 358.0, "evalue": 5.48e-124, "valid": true, "product": "Vaa surface lipoprotein adhesin", "uniref50_id": "UniRef50_O53103" }, "pscc": { "uniref50_id": "UniRef50_O53103", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103" ], "product": "Vaa surface lipoprotein adhesin" }, "genes": [], "id": "LMJBGEANGM_799", "locus": "GCA013368015_00789" }, { "type": "cds", "sequence": "contig_1", "start": 448742, "stop": 449086, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A928KBQ1" ], "nt": "GTGGGAGAAATTAGACCTGCCGGTAGAAATCATTATGATGTATGGACAATTGGCATTTTAAAAAAAATATTGAAAACCGAAAATGCCTCAATAATAAATAATATAGCCACATTATCTTTGGATAATTTGGAAGTTAGAAAGCAAAGGGGAGGGAACAATGAAATATCTGGTTACAAAATAAATCCTTTATTTTTTGTTTATTCAAAAGACTGCATAATTGATAAAAATAGTAATTTAATTTCATTTAACCTAAAAAATGTACAACAATTAAACCCCACCATTGCTGCCAAGATTTTCTTTGAAGAATTAGATTTTGATATTGTTAAAAAACATTACAACTTTTAG", "aa": "MGEIRPAGRNHYDVWTIGILKKILKTENASIINNIATLSLDNLEVRKQRGGNNEISGYKINPLFFVYSKDCIIDKNSNLISFNLKNVQQLNPTIAAKIFFEELDFDIVKKHYNF", "aa_hexdigest": "d2a71c8dc6ca8074b687e33e445b2e66", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI00036C7E1F", "query_cov": 1.0, "subject_cov": 0.8976377952755905, "identity": 0.57, "score": 139.0, "evalue": 2.75e-40, "valid": false, "uniref50_id": "UniRef50_A0A928KBQ1" }, "pscc": { "uniref50_id": "UniRef50_A0A928KBQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A928KBQ1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13117.939899999983, "isoelectric_point": 9.040353202819823 }, "id": "LMJBGEANGM_800", "locus": "GCA013368015_00790" }, { "type": "cds", "sequence": "contig_1", "start": 449096, "stop": 449290, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGAGTGAACACATTGGCTTAAATTTTGTTGAATCTGCTGGTAAAAATATTAATAATTTTTCTTTAAAAAGTATAGAATATGCAATTTCAAATAATTATTTAGAGCCTACTAAAAAGTTTGCTGATGTAATTTGTACAGAAGATCAAAACGGAAATTTAGTAATGATTCCTGCAAATCAAATTTCTTTGTGA", "aa": "MVSEHIGLNFVESAGKNINNFSLKSIEYAISNNYLEPTKKFADVICTEDQNGNLVMIPANQISL", "aa_hexdigest": "03646ecc43fc4b7cefefc8ad672f5799", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7088.9558000000025, "isoelectric_point": 4.75000057220459 }, "id": "LMJBGEANGM_801", "locus": "GCA013368015_00791" }, { "type": "cds", "sequence": "contig_1", "start": 449318, "stop": 449875, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S3KHG4", "UniRef:UniRef90_UPI00158AF0E4" ], "nt": "ATGAAAAATATACCTTTAACTAAAAATCAAAAAGAAATAATGGAAGAATTTTATCAACATTCTCCAATGAAAGTAATTTTTTCAAAAGCAGATCGTAATTTGTTTTGGAATCAAGTAAAACATAAAAATTACAACTTTGATTTAGATTTAATCAATAAAAATTGTCCGTCTCTTTATTGCCAAATAAAAAAGAGTATTGAAACCAATAAAAAAATACAGTCTGCTATATTTAGTGAATGTGTGTTTGCTCAAACGTTTGCGAATATTTTAGAACTAACAAATTTTGTTAATTGTTTGGAGAATGAAAACTTTATTCCAGCGGATATAATTAAATTATTAAATTCATACCATTTAGTCCCAAGATATGCTTATTTTAATAACAATAATACTAGATTGTTAATACAGGCAGGAGGACCAGCGGGCATTGATAGTGCCTTAATAACTGTTATTGATTTAACTATATATACGATAGAATTTAAAGAATCAGCAGCCAAAATGAGTGAAATAGATCTTCCAAAATATGGTGAAGATGGTAAGATGATTGTGACCCCCAAATGA", "aa": "MKNIPLTKNQKEIMEEFYQHSPMKVIFSKADRNLFWNQVKHKNYNFDLDLINKNCPSLYCQIKKSIETNKKIQSAIFSECVFAQTFANILELTNFVNCLENENFIPADIIKLLNSYHLVPRYAYFNNNNTRLLIQAGGPAGIDSALITVIDLTIYTIEFKESAAKMSEIDLPKYGEDGKMIVTPK", "aa_hexdigest": "504e917caa44c92d25371c258d08ec1f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 449015, "stop": 449875, "observations": { "mutation": [ 449098, 449320 ], "stop": [ 449098, 449320 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158AF0E4", "query_cov": 1.0, "subject_cov": 0.4907161803713528, "identity": 1.0, "score": 375.0, "evalue": 1.05e-128, "gene_start": 1, "gene_end": 185, "reference_start": 1, "reference_end": 185, "reference_sequence": "MKNIPLTKNQKEIMEEFYQHSPMKVIFSKADRNLFWNQVKHKNYNFDLDLINKNCPSLYCQIKKSIETNKKIQSAIFSECVFAQTFANILELTNFVNCLENENFIPADIIKLLNSYHLVPRYAYFNNNNTRLLIQAGGPAGIDSALITVIDLTIYTIEFKESAAKMSEIDLPKYGEDGKMIVTPKWLKKNQQYSKMVSEHIGLNFVESAGKNINNFSLKSIEYAISNNYLEPTKKFADVICTEDQNGNLVMIPANQISLWGNIVGEIRPAGRNHYDVWTIGILKKILKTENASIINNIATLSLDNLEVRKQRGGNNEISGYKINPLFFVYSKDCIIDKNSNLISFNLKNVQQLNPTIAAKIFFEELDFDIVKKHYNF" }, "paralog": false, "identity": 0.993006993006993, "score": 571.0, "evalue": 1.55e-208, "description": "Internal stop codon at 449098, 449320. Nonsense mutation around 449098, 449320.", "psc": { "uniref90_id": "UniRef90_UPI00158AF0E4", "uniref50_id": "UniRef50_S3KHG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158AF0E4", "UniRef:UniRef50_S3KHG4" ] }, "pscc": { "uniref50_id": "UniRef50_S3KHG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_S3KHG4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 21311.405299999995, "isoelectric_point": 7.622529411315918 }, "id": "LMJBGEANGM_802", "locus": "GCA013368015_00792" }, { "type": "cds", "sequence": "contig_1", "start": 449896, "stop": 450138, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTATATTTTTTGACGCATTATCAATTGAAAACAGAGAATTTTTAAGTAAATTAACTAATAAGATGTTAGAAGAATCACATAAATACATAATTATAAAAAATAATATTGGCGAACAACAAAATATAAAATTTCCCAAAAAATACAGAGATTTACTAAATAGGATTTTTCTTGACACCCTAGAAATAGAAGAAGACGAAAAAATTTTCGACATTATTCATAGTAATTCAGTATTTAAATAA", "aa": "MPIFFDALSIENREFLSKLTNKMLEESHKYIIIKNNIGEQQNIKFPKKYRDLLNRIFLDTLEIEEDEKIFDIIHSNSVFK", "aa_hexdigest": "1fb08338e8f228fbe76d8fc7152d9bac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9648.033199999994, "isoelectric_point": 5.643736839294434 }, "id": "LMJBGEANGM_803", "locus": "GCA013368015_00793" }, { "type": "cds", "sequence": "contig_1", "start": 450193, "stop": 450372, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAGTTAATAGATGAAAAAGGTAATTATATTATTTGCATGCCTAATACTTGTCGCTATTTTACAGTTGCATCTAATAAAGATATACAAAGAAATGGAAAAATTATTTTAAAATTCTCAAATAAAGAATATTTTGAATATGTATATGCCATGATTAATTCTTCATTTGCTTATTGA", "aa": "MQELIDEKGNYIICMPNTCRYFTVASNKDIQRNGKIILKFSNKEYFEYVYAMINSSFAY", "aa_hexdigest": "463b27a0a233ee4483bf15837acdafea", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7018.0100999999995, "isoelectric_point": 7.728136253356933 }, "id": "LMJBGEANGM_804", "locus": "GCA013368015_00794" }, { "type": "cds", "sequence": "contig_1", "start": 450652, "stop": 450837, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTCAAGAGAATTTTATTCGGCTTTTATGGAAAAAGTATTTTTGCAATCTGAATCGGCTGTTATTATTACGCCATATAGTTTTATTTCGGGCAAAAAATTTTACTCATTAAGGAAAACAATGTGTGAAATAGGCAATGGGTTTATTGTTTCATTTGATAACGTTCCCGGAAATATTTTTTGA", "aa": "MDSREFYSAFMEKVFLQSESAVIITPYSFISGKKFYSLRKTMCEIGNGFIVSFDNVPGNIF", "aa_hexdigest": "458309a35351b387af695931093030fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6988.987, "isoelectric_point": 6.087706184387206 }, "id": "LMJBGEANGM_805", "locus": "GCA013368015_00795" }, { "type": "cds", "sequence": "contig_1", "start": 451174, "stop": 451320, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCAATACACATAATGAATTTTTAAATGTTTCTAACTTTAGCGAAATGTATGAAATTGGTTTAGAAGTTGAAAATAAAATTCAAAAGAAACAATCCGGCCAATATTACACACCAAACGACGTGGCTAAAACAATGGCTAACTGA", "aa": "MFNTHNEFLNVSNFSEMYEIGLEVENKIQKKQSGQYYTPNDVAKTMAN", "aa_hexdigest": "1edd88e16046386b8ee55fd960462453", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5604.176600000004, "isoelectric_point": 5.053861808776855 }, "id": "LMJBGEANGM_806", "locus": "GCA013368015_00796" }, { "type": "cds", "sequence": "contig_1", "start": 451345, "stop": 451491, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGAAATAAAAGAAGCAAAAAAAGTAGATAAGCATAAAGATGATTTTTTTAATTTATCAAAGCAAGATATTGAAAAATTTTCTAAATATAGTTTAGATCAATATGTTTTAGATGTGAATAAAAACGGTTTAAAACAAACGTGA", "aa": "MSEIKEAKKVDKHKDDFFNLSKQDIEKFSKYSLDQYVLDVNKNGLKQT", "aa_hexdigest": "9a4d88e6c1f9339f77ac5851c6ed1cb8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5694.384100000002, "isoelectric_point": 7.96508502960205 }, "id": "LMJBGEANGM_807", "locus": "GCA013368015_00797" }, { "type": "cds", "sequence": "contig_1", "start": 451806, "stop": 452135, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTCATTAAAAACAATTGAAGATAAATATAATTCGGCATTAGAAAAGAAAGATGAGAATGCATTAAAAATAGAAATAATCAAGATGGAAAATTTTTATAAATTAATAAAAACGTTGTTTGCTTCATATTTGAGTGAAATTGCTTCTATTAAATCAATGATGTATGATGATAATAAAAAAGAAGAAGTTTCTAAAAAGGCCATTGAATATGCTAAAAATGCTTTAAATGATTTGATGTCACAAGATATTAGCAATAAAGAATCAATTTCTAAAAATGTGATTGATATTAAAAAAGAATTACATAAAGCATATATTAGTTTATGA", "aa": "MNNSLKTIEDKYNSALEKKDENALKIEIIKMENFYKLIKTLFASYLSEIASIKSMMYDDNKKEEVSKKAIEYAKNALNDLMSQDISNKESISKNVIDIKKELHKAYISL", "aa_hexdigest": "b50ada7fc6b1b68aeff26832e9586997", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12631.461499999987, "isoelectric_point": 7.801722145080567 }, "id": "LMJBGEANGM_808", "locus": "GCA013368015_00798" }, { "type": "cds", "sequence": "contig_1", "start": 452136, "stop": 452633, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTAAAATTGATGGTCATGAGGATGCTATCGATCGATATTTAAAAATATTTGAATCTAATGAAATATTTGAATCTAATGAAAATAATAGAGAGTTTGTTACCAAACTTAGAACAAAAGTAGAAAAAATCAAGGAATTATATAAAAAATCTAAAAGTCAAAATCGAAAGAAAGTAGCAGAATATTTAAAAAATCTCTCAATACTTTCTATACTATTGTTCCAATATAACTATTATTGTAAAATATATTATCGTGAATTACCAGCAACTCTACAAGGAAAGCTTATTAAAGAATTTAAAAAAATATTAGATAAAGATAGCTTTCCTAGCGATTCTGAGATAACTGAATTAGAAAATAAATCAATAGAGTTAATGATAGCTGAAGATCCAAAAATCGCATTTACCATTCTTCAAAAGCAAACAAATGATTTAAAAGAATTTGTACAAATTTATGTTAAAGAATCGCCAGATAGTGAATCTAATAATTTTCATACATGA", "aa": "MRKIDGHEDAIDRYLKIFESNEIFESNENNREFVTKLRTKVEKIKELYKKSKSQNRKKVAEYLKNLSILSILLFQYNYYCKIYYRELPATLQGKLIKEFKKILDKDSFPSDSEITELENKSIELMIAEDPKIAFTILQKQTNDLKEFVQIYVKESPDSESNNFHT", "aa_hexdigest": "85b510628ef9c25b8612946307bcc15c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19663.2686, "isoelectric_point": 7.709834098815918 }, "id": "LMJBGEANGM_809", "locus": "GCA013368015_00799" }, { "type": "cds", "sequence": "contig_1", "start": 452667, "stop": 456038, "strand": "-", "frame": 1, "gene": null, "product": "Variable membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8RJN9", "UniRef:UniRef90_UPI0034A3A5D5" ], "nt": "ATGAAAAAGAAATTAAATATTTTACTTTCTATTTCACTAATAGCCGCAGCTGCTTCGACTGCTGCTATAGCATCAGTATGTGCTTCAAAAAACAATAAATTTAGAAAGCAAAGAAAATACAATAAAGAAACAGCAATACAAGAAATAAATCAAGCAAAAGAGAATTTAAAGAAATTACTAGAACTAATAAAAGCAAGTGATATTGATAAATCAAATGAATCAAAAGTTTTAGATAATTCTAAAATTGATGAAAATTCTTCAATTGAAGATATTCAAAATAAAACCCAAGAAATAGAAAAAGCAATTGAATCATTAACTAAAAAAATTAATGATAAAAACAAAAGACACGAAGAAGATCAAAAACTCGTGCAAGCAATGCAAGAATTTAGAAAATCCCAAAAAGCTTTGGGAGATTTAATTAATTCAGAAGATGGCCAAAGAGTTGACAATTCAAATGCAAAACAATCATTACAAAATAATACAGTCAACAATAAATCATCGATAGAACAAATTATTCAGGCAGTTTCTAAAATCAATAAAGCTAAACAAGAACTTCAAAGTCAAATTAATAATGCTAGAAACCAAGCAAAAGAAGTTTTTGAAGAAAAGAAACAACAATTGAACAAATTAATCAAATCAGACGAAATCGATAATTCTAAAAAAGCGGATGAAACTGCAATTTTAAAAAATACAAATGTTGTAGTGGGTGATTCAATTAAAACAATTGAAACTAAAACCAATGAAATAGAAAAAGCAATTGAATCATTGACTAACAAGATTAATGAATTCAAAAAGGAACAAGAAAAAGCAAATGCCAAAGCAGTTTTTTTAAAAAAGAGCAAACAACTAAAAGATTTAATTGATTCAGAAGATGGCAAAAAAGTTGATAGTTCTAATGAAAGTCAAGTTTTAACTAAAACAAAGATTGATGAAAATTCTTCAATTGGAGATATTCAAAATAAAACCAAAGATATTGAAAAAGCAATTGAATCATTAACCAACAAGATTAACGATCAAAAGCAACAAAAAAATATGCTTAACGAAGTAATTAACAAAGCAAAAGAATTAGTTAAAAAATTAATTGATAGTGATAGCGAAATTCAACAAGCAAAAACTGAATTAGAAAAAGAAGTTCAAAAAGCTAGTCAAGTAGTTAATTCAAATGATACAAAGGCAATAAATTCTTCAAGAGCATCACTTGATGCAAAAATTAACGACATTGCCAAAAAATTAGAAGCATTTAATGCAACTAAAAAATTAGAATTTACTAAATTGCAAGAAACAAGATCAAATATTGATAAATTCTTAACTCCCGAAGTTAAGACAAATCCAAATTACACAACCTTAGTTAATGAATTAGAAGTTGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGACATTGTCGCTTCAAATAACGAATTAAAACAAGCTTTGCAAACAGCTCAATCTTCTAAAAATGATGCCGATAAAACATCTAGTGAAGAAAAAGCTAAATTGTCTGCAAGTTTATCTAATGCGAAAGAATTAGATAAAAACCTAACTGATAGTGACGGTGAAATTCAACAAGCAAAAGCCGAACTAGCAAAAGAAGTTGAAAAAGCAAATCAAGCAATTACTTCAAACAATACAAAAGAAATACAAAATTCAAATACTTCATTATCAAATAAAATTTCTGAAGTTAAAAATAAATTAGATAAATTTAATAATGAAAAAGAAGCAGAATTTAATAAATTAGAAGCATCTCGTAGTGCAATTAATGAATTTATTAACGAAAATAATACCAATCCAAATTACACAGCATTAATTCAAAAATTGCAAGCAACATTGGATGCTAAAAATAGTATTACTAAATCTTCAAATAAATCAGACATTGTCGCTGCAAATCAAGCCTTGCAAGAAGCATTGGCTATTGCAAAAAACGAAAAAGAAAGTGCAAATAGCCAAAATCAAGCTATAAAAAATACATTAAGTGAAACTATTGGCAAGGCAAAAGAATTAGATAAAAACCTAACTGATAGTGATAGTGAAATTCAACAAGCAAAAACTGAACTAACCAATGAAATTGAAAAAGCTAATCAAGCAATTGCTTTAAATAATACAGCTTCAATGGAAGGTTCAAATACTTCATTATTAAATAAAATTTCTGAAGTCCAAAATAAATTGGATAAATTTAATAATGACAAAAAAGTGGAATTTAATAAATTACAAGAATTAAAAAATAAAATAGATGATTTTGAAAAGAAAAACAAAAATAATGAAATTTATTCAAAATTCAACTTAGACGAGTTGATTAATAAATCAATTCAAATAAAAAATAGCCTAGGTTCAATAAATGAATCTTCAAACAAAAAAGATATTGCTAATGCTAATAAAAAAATGCAAGATGCTTTAGATGAATTACAAGCCAAAATGGCTGAAATTCATAAAAAAACATTTCAAGAATTTAACGAACATAAAAATGAATTAGAAAATCTTATTAAAACAGAAGATGCAAACGAAGTTGGCACAGATGAAGCAAATACGGCAATAACTAACAATAATGTCAAAGAAGATTCATCTATTGAAGAAATAACAAAGGCAACCAAAGCACTTGATGAAGCAAGATCTAAATTAGACCAAAAAATTAAGACTCAAAAAGCAACCGAACTTAACAGCCTTAATAAATCAAAAGAAAAACTAAATACTTTAATTACCTCCTCAAGTAATCAAGTGTCAGCTGAAGAAATATATAAGGCTAAAAAGGTGTTAGAAGAAATTAATAATTTAAGTCTAAATAACGATTCCTCAATAAAATCATTGAAAGAAGCAACTCAAAAAATTAAGGATGCAGAAACACAACTAACCAAAGAAGTTGAAAAAGCAAAAATAGAAAAAACTGATAAATTGAAAAAATTCAATGAAGTAAAAAAATCTTTAGAAGATTTAATTAAAGATGATGATGCAATTCAAGTAGGAACAGATGACGCCCCTAAGTTATTAGAATATAATAACAAGATTAATGAAAATTCATCTATTGAAGAAATAACAAAGGCAACTAAGGCACTTGAAGTTGCAAAATCTAAATTAGACCAAAAAATTAAAACAGAAAAACAACCACTGATACGTGATTTAAAAAGAAAAGTAGATGATTTAAATAGATGGCTAAATAATGGTGGTCAAAATTCAGAAGAAAATTTCGCTGCTCTTGATCCTGATAAAAGACCTAAAAACGTAACCCTAGATACAATTAAAAAAGATCTCGAAGATGCTAGAATATTATTACAAGAAGCAACCGAACTTACTGATGATTCAAAAATTGCTAATATAAATAAAAAAATTGGTGAAATAGAAGAGTTAAATAAAAAGACGGATCTCTGA", "aa": "MKKKLNILLSISLIAAAASTAAIASVCASKNNKFRKQRKYNKETAIQEINQAKENLKKLLELIKASDIDKSNESKVLDNSKIDENSSIEDIQNKTQEIEKAIESLTKKINDKNKRHEEDQKLVQAMQEFRKSQKALGDLINSEDGQRVDNSNAKQSLQNNTVNNKSSIEQIIQAVSKINKAKQELQSQINNARNQAKEVFEEKKQQLNKLIKSDEIDNSKKADETAILKNTNVVVGDSIKTIETKTNEIEKAIESLTNKINEFKKEQEKANAKAVFLKKSKQLKDLIDSEDGKKVDSSNESQVLTKTKIDENSSIGDIQNKTKDIEKAIESLTNKINDQKQQKNMLNEVINKAKELVKKLIDSDSEIQQAKTELEKEVQKASQVVNSNDTKAINSSRASLDAKINDIAKKLEAFNATKKLEFTKLQETRSNIDKFLTPEVKTNPNYTTLVNELEVAKKAKESVSESSNKSDIVASNNELKQALQTAQSSKNDADKTSSEEKAKLSASLSNAKELDKNLTDSDGEIQQAKAELAKEVEKANQAITSNNTKEIQNSNTSLSNKISEVKNKLDKFNNEKEAEFNKLEASRSAINEFINENNTNPNYTALIQKLQATLDAKNSITKSSNKSDIVAANQALQEALAIAKNEKESANSQNQAIKNTLSETIGKAKELDKNLTDSDSEIQQAKTELTNEIEKANQAIALNNTASMEGSNTSLLNKISEVQNKLDKFNNDKKVEFNKLQELKNKIDDFEKKNKNNEIYSKFNLDELINKSIQIKNSLGSINESSNKKDIANANKKMQDALDELQAKMAEIHKKTFQEFNEHKNELENLIKTEDANEVGTDEANTAITNNNVKEDSSIEEITKATKALDEARSKLDQKIKTQKATELNSLNKSKEKLNTLITSSSNQVSAEEIYKAKKVLEEINNLSLNNDSSIKSLKEATQKIKDAETQLTKEVEKAKIEKTDKLKKFNEVKKSLEDLIKDDDAIQVGTDDAPKLLEYNNKINENSSIEEITKATKALEVAKSKLDQKIKTEKQPLIRDLKRKVDDLNRWLNNGGQNSEENFAALDPDKRPKNVTLDTIKKDLEDARILLQEATELTDDSKIANINKKIGEIEELNKKTDL", "aa_hexdigest": "e3b9359e8bbed440bf7a83a84ce48377", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 452364, "stop": 456038, "observations": { "mutation": [ 452669 ], "stop": [ 452669 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A3A5D5", "query_cov": 1.0, "subject_cov": 0.7919605077574048, "identity": 0.9990000000000001, "score": 1742.0, "evalue": 0.0, "gene_start": 1, "gene_end": 1123, "reference_start": 1, "reference_end": 1123, "reference_sequence": "MKKKLNILLSISLIAAAASTAAIASVCASKNNKFRKQRKYNKETAIQEINQAKENLKKLLELIKASDIDKSNESKVLDNSKIDENSSIEDIQNKTQEIEKAIESLTKKINDKNKRHEEDQKLVQAMQEFRKSQKALGDLINSEDGQRVDNSNAKQSLQNNTVNNKSSIEQIIQAVSKINKAKQELQSQINNARNQAKEVFEEKKQQLNKLIKSDEIDNSKKADETAILKNTNVVVGDSIKTIETKTNEIEKAIESLTNKINEFKKEQEKANAKAVFLKKSKQLKDLIDSEDGKKVDSSNESQVLTKTKIDENSSIGDIQNKTKDIEKAIESLNNKINDQKQQKNMLNEVINKAKELVKKLIDSDSEIQQAKTELEKEVQKASQVVNSNDTKAINSSRASLDAKINDIAKKLEAFNATKKLEFTKLQETRSNIDKFLTPEVKTNPNYTTLVNELEVAKKAKESVSESSNKSDIVASNNELKQALQTAQSSKNDADKTSSEEKAKLSASLSNAKELDKNLTDSDGEIQQAKAELAKEVEKANQAITSNNTKEIQNSNTSLSNKISEVKNKLDKFNNEKEAEFNKLEASRSAINEFINENNTNPNYTALIQKLQATLDAKNSITKSSNKSDIVAANQALQEALAIAKNEKESANSQNQAIKNTLSETIGKAKELDKNLTDSDSEIQQAKTELTNEIEKANQAIALNNTASMEGSNTSLLNKISEVQNKLDKFNNDKKVEFNKLQELKNKIDDFEKKNKNNEIYSKFNLDELINKSIQIKNSLGSINESSNKKDIANANKKMQDALDELQAKMAEIHKKTFQEFNEHKNELENLIKTEDANEVGTDEANTAITNNNVKEDSSIEEITKATKALDEARSKLDQKIKTQKATELNSLNKSKEKLNTLITSSSNQVSAEEIYKAKKVLEEINNLSLNNDSSIKSLKEATQKIKDAETQLTKEVEKAKIEKTDKLKKFNEVKKSLEDLIKDDDAIQVGTDDAPKLLEYNNKINENSSIEEITKATKALEVAKSKLDQKIKTEKQPLIRDLKRKVDDLNRWLNNGGQNSEENFAALDPDKRPKNVTLDTIKKDLEDARILLQEATELTDDSKIANINKKIGEIEELNKKTDLWERAIEYELEDLMRKIDGHEDAIDRYLKIFESNEIFESNENNREFVTKLRTKVEKIKELYKKSKSQNRKKVAEYLKNLSILSILLFQYNYYCKIYYRELPATLQGKLIKEFKKILDKDSFPSDSEITELENKSIELMIAEDPKIAFTILQKQTNDLKEFVQIYVKESPDSESNNFHTWMNNSLKTIEDKYNSALEKKDENALKIEIIKMENFYKLIKTLFASYLSEIASIKSMMYDDNKKEEVSKKAIEYAKNALNDLMSQDISNKESISKNVIDIKKELHKAYISLWHKFCLND" }, "paralog": false, "identity": 0.9950980392156863, "score": 2114.0, "evalue": 0.0, "description": "Internal stop codon at 452669. Nonsense mutation around 452669.", "psc": { "uniref90_id": "UniRef90_UPI0034A3A5D5", "uniref50_id": "UniRef50_Q8RJN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A3A5D5", "UniRef:UniRef50_Q8RJN9" ] }, "pscc": { "uniref50_id": "UniRef50_Q8RJN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8RJN9" ], "product": "Variable membrane protein" } }, "id": "LMJBGEANGM_810", "locus": "GCA013368015_00800" }, { "type": "tRNA", "sequence": "contig_1", "start": 456468, "stop": 456544, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 59.6, "nt": "GCGTTTGTGGCGAAGTGGTTTAACGCTCCGGGTTGTGGCTCCGGCATACGTGGGTTCGATCCCCATCAAACGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 456536, 456538 ], "id": "LMJBGEANGM_27", "locus": "GCA013368015_00801" }, { "type": "cds", "sequence": "contig_1", "start": 456741, "stop": 457589, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD515", "UniRef:UniRef90_UPI00193AD515" ], "nt": "ATGCTTAAAATAAAAGGTGGTTTAGTTTCAGTAATTAATTCAAATATAGAGCAACTTAAGAAATTTAAGGCAAAAAAATTTAATAATAATTTTATTTCAAAACTTAATGAAAAATTAAACAGTAATATTTTTAATAATCTTTCGAGAAATATGAAATTAGCCGATCTTAAAAAAATAGAAGAAGATTCAGTAAATCTTTTAAGGCTTGTCAAATTACAAGAAAAATATGAAAATGACGATAAATTGTCACCGTCTCTGCAAAAAAAACTTGTAAAAGAATTTGAAAAAATATTAGATAAAGAAACAATACCTAGTGATTCTGAAATAACTGAATTGGAAAACAAATTAGAAAAAATTATTGAAGAAGATCCAACTACGCCATTTACTACAGCATTTAGTAATCTTCAAAAGGAAACAAAAAATACTAAGGAATTAATAGAAAATCTAGTCCCACAATCTGTTAAAGAAGAACTTGATGGATTCAAAAAACTTCAATCATGTATTAATGATGCATTGAAAATGATTGGAGATGGATATAATTCAGCATTACAAAAAGAAGATTTGGATGCCCTAAAAGTAGAAACAAGCAAAATAAACAATTTAAATGAATTAATTAAATTGTTTTATACATATTGGGAACAATTTAAATATTTAATTTCTGATAAACATGATCAAAGAGAAGTCTTTAAAAGAGGAATTAAAGATGCTGATGAAGCAATAAATGCCTTGGGACAAATTAATTTAAATCAAGACCTTGGTAATGATAAATTAATGAAAGAAAAAATCAAAGATATTGAAGAAAAATTAGCAACGGCTCAATCTTTAACGAAAGATCATTTCAAACGTTAA", "aa": "MLKIKGGLVSVINSNIEQLKKFKAKKFNNNFISKLNEKLNSNIFNNLSRNMKLADLKKIEEDSVNLLRLVKLQEKYENDDKLSPSLQKKLVKEFEKILDKETIPSDSEITELENKLEKIIEEDPTTPFTTAFSNLQKETKNTKELIENLVPQSVKEELDGFKKLQSCINDALKMIGDGYNSALQKEDLDALKVETSKINNLNELIKLFYTYWEQFKYLISDKHDQREVFKRGIKDADEAINALGQINLNQDLGNDKLMKEKIKDIEEKLATAQSLTKDHFKR", "aa_hexdigest": "6879f4f97dde8a7e9a0a515666bba302", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 456744, "stop": 457889, "observations": { "mutation": [ 457601 ], "stop": [ 457601 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AD515", "query_cov": 0.9964539007092199, "subject_cov": 0.4390625, "identity": 0.8079999999999999, "score": 412.0, "evalue": 2.98e-138, "gene_start": 1, "gene_end": 281, "reference_start": 359, "reference_end": 639, "reference_sequence": "NNKQAPADAISAAQKTLDEIDKLNLTKVSTIKSLKDATQKINAANEALKQAIEKLEAEKTKELQKFNEAKKALEDLIKDEDAKEVDITEATAAITKNSADKNSTLDEITKATKALEDAKSKLDQEIKTQKAAEFNNLTNAKAELSTLITKQAPAEAISKAKKVLEEINSLSLNNDSSIKSLKDATQKIKDAETQLTAEIEKTEKLKEFDEARKSLRELIKDDDAKEVETSKAKEALKTNKADENSSIEEIIKATKALEDAKFELNQEIRVQKDRLMDKLNAKNKKLEALLNTENLDDLMSIEGLDGLSADEIKNKLENAKKLLQEAKGLSENSKITNIKQKIQKIDEFVGKLESWISNMLRIRKGLVTVIKSNIDQLKEFKAKKFNKDFISKLNEKLNSNIFNNLSINMKLADLKKIEEDSKNLLRLVKLQEKYENDNKLSSTLQKKLVKEFENILDKETIPSDSEITELEDKLKKMIEEDPTTEFTTAFSNLQKETKNTKELIENLVPQSVKEELAGFKNLQCKMNQALKMIEDGYNSALQEENLDALKVETSKINNLNKLIKLFYGYWKQFNHFISDNHTQEEVFRRGIKDANKAIETFLSINLNQEIGNDKLMNEKAKEIENELATAQSLTESHFKH" }, "paralog": false, "identity": 0.8398950131233596, "score": 549.0, "evalue": 2.68e-194, "description": "Internal stop codon at 457601. Nonsense mutation around 457601.", "psc": { "uniref90_id": "UniRef90_UPI00193AD515", "uniref50_id": "UniRef50_UPI00193AD515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AD515", "UniRef:UniRef50_UPI00193AD515" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AD515", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AD515" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 32645.045600000027, "isoelectric_point": 6.802513313293456 }, "id": "LMJBGEANGM_811", "locus": "GCA013368015_00802" }, { "type": "cds", "sequence": "contig_1", "start": 457599, "stop": 462785, "strand": "-", "frame": 1, "gene": null, "product": "Variable membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Y3" ], "nt": "ATGAATAAGAAAATAGGTATTTTACTTTCAATTTCTTTGTTAGCCACAGCAGCTACAACTGCTACTATAGCAGCCATTGCCGCAAAAGGAAAATTTAAAAAACAACAAGAATTAAAGCAAAATATTGAAAAATCTAAGGCCGAACTAAATAAGGCCAAATCTATTTTAGACAAATTGCTAAAATCAGATTATGCTAAAAATGTTAATAAATTAGAAGAATCTAAAATTTTAGATAATGCTAAAATTGATGAAAATTCTTCACTTCAAGACATTAAAAATAAAACGAAGCAAGTAAAAGAAGCAATTAATTCATTAACTCAAAAAATTCTTGAAAGAAAACAAAAAGAACAACAAAATCAAAACGATAAAAAAACTCTTGATAAACTAAGATTAGATTTTGCAAACGCCAAAGAAGAATTACAAGCATTGATTAATTCAAAAGATGGAAAATCAGTAGATACCACCAAGGCCACTGAAGTATTAAAAAATACTAAAATAACAAATTCATCTGCTACCTTTCAAGTTCTTCAAGCAGTTCAAACAATTAAAAAAGCTACAAAAGAATTGCAAGAAGCAATTAAATTTAAGAAGCAACAAGAATTTAATGAATTTAATAAAATAAAGCAAGAATTAACAGATTACATTGCAAACGATTTAAAAGATTTGAAATACAAAGATATTAAGGCAAAGGCACAAAAATCAATTGAAAAAGTTAGCGATATAAACATCAATAGTTCTTTTATTAGCGATATTCAACAAGCAACTAAATTATTAATTGATGCTAAAAAACAAGCAATCATAGAAGTAGAAAAAATCAATAAAAGTAGTGAATTAGACAAATATAATATTGTTAAAAGTAAATTAGAACAATTAATTAAAACTGAAGATGCTCAAATGATAAAAAGTGCCAATGAAAAAGCTAAAAAGATATTAGCAGAAAACACGACAAAGGATACCACATTATCATCTGAAGAATTCAAGACAAGAAGAATAAATCTTGAAAACGCTCTAGAAGAACTTAAAGAAGCAATTAAAAATGAAAAGAATATTCAAAAACAAGAATTTGAAGCAAAAAAACAAGAATTAAAGACATTGCTAGAATCAAAATGGTCAAAAGATATTGACAAGAAAAATGAAACAACAGTCTTTAATAATGCAAAGATTGATGATAGCGATTCAATAAAAGAAATCAAGGAAGCAACAAAAGAAGTTCAAGGTGCTATTGATTCATTGATCCAAAAAATGGATGAAAAAAATAAATTAAATCCTTCATATGCAAAAGAAAAATTTGAAAAAACTCAAAAACAATTGCAAGATTTAATCAATTCAGAAGATGCAAAATTCATCGATACTAATAAAGCATCCGAAACATTAAAAAATACTAAAATAACAAGCACTTCTTCAATAGACGAAATAAACCAAGCAATTAAAACTCTTAAAGATGCAATCGTTGAATTGAAAAATGCAATAAATGGTGCTAAAGAAGATTCAATAAAAGAATTTGATAAAGTTAAAAAAGATCTAGAAGTTTTAGTAGGCTCAGAAGATGCAAAACCAATTGAAAAAAGAACTGAAATTGGTATTTTGGCAAACACTTATATTCTTGAAAGAGATACAATAAAACAAATTAAAGAAAAGACTGAAGCAATTAAAAAAGCAATATTATCTTTAAACAAAAAAATTGCAAACGTTAAGGCATTAAAAAATGAGTTGCAAAAATTTAATGATATTAAGGTTGCTTTAGAACAATTAATTAAAACTGATGATGCAAAAGAAGTTGGAACAGAAGAAGCATCAAAAGCTCTTGATAATAATAAAGTGAATGAAAATTCAACTCTTGAAGAAATAACAAAGGCAACTAAAGCACTTGAAGATGCAAAATCTAAATTAGATCAAGAAATCAAGGCTAAAAAAGAAACTGAATTTAATAATTTAACAGTTGAAAAAGGCAAATTAAATGAATTAATTGCTAAATCAACTGATGCGTCAGCAAAAGCAATTGAAGAAGCCAAGAAAGTATTGACAGAAGTTGAAAAATTAAATAACTCCTCACCAATTAATTTACTAAAAAATGCAGTTATAAAAACAAAGGACGCTATAGAAAAATTAAATAATAATGTAGCCCAAGAAAAAGACAAAAAAGCCAAATTAGTTGAATTTAGTAGTATAAAAACACAATTAGAAGAATTGATTGCAAAAGATGATGCAATTCAAGCTGGAGTAAAAGAAGCCAAAAAAGCATTGCAAGATAATAAGGCTGATGAAAATTCGGCCGTTGAAGAAATAACAAAGGCAATCAAAGCATTGCAAGAAGCAAAAGTAGCATTGGAACAAAAAATTTCAAATGCTCAAGAAAAAGCTAAACAAGAATTTGATACTAAAAAAGAACAATTAAAAACATTAATTGGCTCAAGTGATGCAAATAATATTAGTGATAAAACTAATGAATTAAATGTTTTAACTAATACTTCAATTTCTAAAACAGATTCTATTAAAGAAATTAAACAAAAAACTTCAACTATTGAAGATGCAATTAAGACCTTAAATAAAAAAGTTCAAGATAAAAAGAACGAAAAAATTCAAGAATTTGAAAAGGCTAAAGAAAGTCTAAAAAAACTAGTTAAAGAAGAAGATGCAAAAGAAGTTGGCATTGATAGTGCAAACGAAGCTTTAAAAACAGAAGTTGATGAAAAATTAACTATTGAAGAAATTAAAAATGCTATTTCAAAAATATCTAGTGAAGAAACAAAATTAAAAAGTAGCATTGAAAGTGCAAAACAACAAGCTAAAGAAACATTTGATTCAAAGAAGCAAGAATTAAAAAGCCTTTTAGATTCTGCAACATCTGAAGTTGATAAAAAAGTTGAAGAAGAAAAATACAATAAAACTACAATTGATAATGATTCTACAATTAAACAAATTAAACAAAAGACAAGTGACATCGAAAAAGCAATAACAACCTTAAAAGAAAAAATAGCCAAAGTAAAAGGAGATAAGGCTGTTGAATTACAAAAATTTAATCTAGTAAAACAACAATTAGAAGAATTGATTGCAAAAGAAGATGCAAAAGAAGTTGGAACTTCTAAAGCTGAAGAAGCATTGAAAAATACTGTAGTAGACAAAAATTCAACTATTGATGCTATTTCAAAAGCAACTAAAGCTCTTGAAGGTGTTAAAACTGAATTAACACAAAATATTATTGATGCTAAAAATAATGCAACAAAAACATTCAATGACAAGAAAGATGAATTAAAGAAATTACTAGAATTAAGTGATGCCAAAAGTGTTGATAATAAAAAAGAATCTGATGTTTTAAAAGATAATTCAATTGATTCAAATACTCCAATAAAAGAAATAAAAACCAAAACAGAAAAAATTACAGAAGCAATTAATTCTTTGACTACAAGTATAAAAAATAAAAAGGATGAAGAATTTAATAAATATAATGATATTAAAACATCATTAGAAAACTTAATCAAAGAAGAAGACGCAGTTCAAGTGGGCATAGCTGATGTTCAAAAAACCTTATCCGAAAACAATGTAGATAAAACTACAACTATTGAAAAAATTCAACATTCAACTGAAGCATTAACTCACGCTAAAGAAGAACTACAAAAATTAATTGACAATACAAAAAAACAATTAACTAACGAATTTGAAACTAAAAAATCTGAATTAGAAAAATTAATTTCCTTACCAGATGCAAATAATGTTGATAAAAAGGATGAATTAAGCATATTTGGCAATACTAGCATTACTAATAGCGATTCTATTAAACAAATAAAAGAAAAAATAGTAAAAATCCAAAATGCTCTTGAATCATTAACTAATAAAATTTCAAAACAAAAAGAACAAGAATTAGAAAAATACAATACCACAAAAACAGCGCTAGAACAATTAATTAATACTGATGACGCAAAAGAAGTTGATACAACCGAAGCTACTGCAGCATTAACTAAAAACAGCGCTGATAAAAATTCAACTCTTGAAGAAATTACAAAAGCAACTAAAGCACTTGGAGATGCAAAATCTAAATTAGATCAAGAAATCAAGACTAAAAAAGAAGCTGAATTTAATAATTTAACTAAAGCAAAAACAGAATTAAGTAATTTAATTACTAAACAAGCACCAGCTGAAGCAATATCTAATGCTAAAAAAGTATTAGAAGAAATTAACGGTTTAAGTCTAAGCAATGATTCCTCAATAAAATCATTGAAAGATGCAACTCAAAAAATTAATGCTGCAAATGAAGCATTAAAACAAGCTATAGAAAAATTAGAAGCAGAAAAAACCAAAGAATTGCAAAAATTCAACGAAGCAAAAAATGCCTTAGAAAACTTAGTTAAAGAAGAAGATGCAATTCAAACTGGAGTAGATGTAGCTAAAAAAGCCTTAGAAGATAATAATGCTAATGAAAATTCAACTCTTGAAGAAATTACAAGCGCAACTAAAGCACTTGGAGATGCAAAATCTAAATTAGATCAAGAAATCAAGACTAAAAAAGAAGCTGAATTTAATAATTTAACTGACGCAAAAACAGAATTAAGTAATTTAATTAAATCTAATAATAAACAAGCACCAGCAGATGCAATATCTGATGCTCAAAAAACATTGGATAAAATTGATAAATTAAACTTAACTAATGTTTCAACAATAAAATCATTGAAAGATGCAACTCAAAAAATTAAGGATGCAGAAACACAACTAACCGCAGAAATAGAAAAAACTGAAAAATTAAAAGAATTCGGCGAAGCAAGAAAATCTTTAAGAGAGTTAATTAAAGACGATGATGCAAAAGAAGTTGAAACTTCTAAGGCTAAAGAAGCATTGAAAACTAATAAAGTAGATGAAAATTCATCTATTGAAGAAATTATAAAAGCAACTAAGGCACTTGAAGATGCAAAATTTGAATTAAATCAAGAAATTAGGGTTCAAAAAGATAGATTGATGGATAAATTAAATGCAAAGAATAAAAAATTAGAAGCGTTATTAAATACTGAAAATTTAGACGATCTTATGTCTATTGAAGGGCTTGATGGCTTAAGTGCAGATGAAATAAAAAATAAACTTGAAGATGCTAAAAAATTATTGCAAGAAGCAAAAGGTCTTTCTGAAAATTCAAAAATTACTAATATAAAACAAAAAATTCAAAAGATAGATGAATTTGTTGGAAAACTTGAATCTTGA", "aa": "MNKKIGILLSISLLATAATTATIAAIAAKGKFKKQQELKQNIEKSKAELNKAKSILDKLLKSDYAKNVNKLEESKILDNAKIDENSSLQDIKNKTKQVKEAINSLTQKILERKQKEQQNQNDKKTLDKLRLDFANAKEELQALINSKDGKSVDTTKATEVLKNTKITNSSATFQVLQAVQTIKKATKELQEAIKFKKQQEFNEFNKIKQELTDYIANDLKDLKYKDIKAKAQKSIEKVSDININSSFISDIQQATKLLIDAKKQAIIEVEKINKSSELDKYNIVKSKLEQLIKTEDAQMIKSANEKAKKILAENTTKDTTLSSEEFKTRRINLENALEELKEAIKNEKNIQKQEFEAKKQELKTLLESKWSKDIDKKNETTVFNNAKIDDSDSIKEIKEATKEVQGAIDSLIQKMDEKNKLNPSYAKEKFEKTQKQLQDLINSEDAKFIDTNKASETLKNTKITSTSSIDEINQAIKTLKDAIVELKNAINGAKEDSIKEFDKVKKDLEVLVGSEDAKPIEKRTEIGILANTYILERDTIKQIKEKTEAIKKAILSLNKKIANVKALKNELQKFNDIKVALEQLIKTDDAKEVGTEEASKALDNNKVNENSTLEEITKATKALEDAKSKLDQEIKAKKETEFNNLTVEKGKLNELIAKSTDASAKAIEEAKKVLTEVEKLNNSSPINLLKNAVIKTKDAIEKLNNNVAQEKDKKAKLVEFSSIKTQLEELIAKDDAIQAGVKEAKKALQDNKADENSAVEEITKAIKALQEAKVALEQKISNAQEKAKQEFDTKKEQLKTLIGSSDANNISDKTNELNVLTNTSISKTDSIKEIKQKTSTIEDAIKTLNKKVQDKKNEKIQEFEKAKESLKKLVKEEDAKEVGIDSANEALKTEVDEKLTIEEIKNAISKISSEETKLKSSIESAKQQAKETFDSKKQELKSLLDSATSEVDKKVEEEKYNKTTIDNDSTIKQIKQKTSDIEKAITTLKEKIAKVKGDKAVELQKFNLVKQQLEELIAKEDAKEVGTSKAEEALKNTVVDKNSTIDAISKATKALEGVKTELTQNIIDAKNNATKTFNDKKDELKKLLELSDAKSVDNKKESDVLKDNSIDSNTPIKEIKTKTEKITEAINSLTTSIKNKKDEEFNKYNDIKTSLENLIKEEDAVQVGIADVQKTLSENNVDKTTTIEKIQHSTEALTHAKEELQKLIDNTKKQLTNEFETKKSELEKLISLPDANNVDKKDELSIFGNTSITNSDSIKQIKEKIVKIQNALESLTNKISKQKEQELEKYNTTKTALEQLINTDDAKEVDTTEATAALTKNSADKNSTLEEITKATKALGDAKSKLDQEIKTKKEAEFNNLTKAKTELSNLITKQAPAEAISNAKKVLEEINGLSLSNDSSIKSLKDATQKINAANEALKQAIEKLEAEKTKELQKFNEAKNALENLVKEEDAIQTGVDVAKKALEDNNANENSTLEEITSATKALGDAKSKLDQEIKTKKEAEFNNLTDAKTELSNLIKSNNKQAPADAISDAQKTLDKIDKLNLTNVSTIKSLKDATQKIKDAETQLTAEIEKTEKLKEFGEARKSLRELIKDDDAKEVETSKAKEALKTNKVDENSSIEEIIKATKALEDAKFELNQEIRVQKDRLMDKLNAKNKKLEALLNTENLDDLMSIEGLDGLSADEIKNKLEDAKKLLQEAKGLSENSKITNIKQKIQKIDEFVGKLES", "aa_hexdigest": "ccf55515562178d09c788e7bcc6e4d78", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_Q700S8", "query_cov": 1.0711805555555556, "subject_cov": 0.8533886583679114, "identity": 0.773, "score": 2202.0, "evalue": 0.0, "valid": false, "product": "Variable membrane protein", "uniref50_id": "UniRef50_A0A454C9Y3" }, "pscc": { "uniref50_id": "UniRef50_A0A454C9Y3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9Y3" ], "product": "Variable membrane protein" }, "genes": [], "id": "LMJBGEANGM_812", "locus": "GCA013368015_00803" }, { "type": "cds", "sequence": "contig_1", "start": 463155, "stop": 463475, "strand": "-", "frame": 1, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "COG:COG0537", "COG:FR", "RefSeq:WP_012855622.1", "SO:0001217", "UniParc:UPI0001BC232C", "UniRef:UniRef100_D1J8D6", "UniRef:UniRef50_P32083", "UniRef:UniRef90_D1J8D6" ], "nt": "ATGCATGATGATATTTTTGAAAAAATAATAACAAGAGAAATCCCAGCAAAAATAATATATGAAGATGAAAAATTTATTGCCTTTGAAGATATTAAACCAGTAGATAAAGGCCATTTTTTAGTAACGGTAAAAAACCACGCCACAAATTTATACGATATTGATGACCAAGCATTGTCATCACTAATATTAAAGGCGAGAGAACTTGCTTTAAAAAGAGTTAAGGAATTAGGTGCAACTGGATTTAATTTAATAATAAATAATAATCTTTCAGCAGATCAAACAGTATTCAGAATTCATGTTCATATAATCCCAAGATATTAA", "aa": "MHDDIFEKIITREIPAKIIYEDEKFIAFEDIKPVDKGHFLVTVKNHATNLYDIDDQALSSLILKARELALKRVKELGATGFNLIINNNLSADQTVFRIHVHIIPRY", "aa_hexdigest": "032e4d9f517db52ca0cbb90f5dbef90e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC232C", "ncbi_nrp_id": "WP_012855622.1", "uniref100_id": "UniRef100_D1J8D6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC232C", "RefSeq:WP_012855622.1", "UniRef:UniRef100_D1J8D6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8D6", "uniref90_id": "UniRef90_D1J8D6" }, "psc": { "uniref90_id": "UniRef90_D1J8D6", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_P32083", "cog_id": "COG0537", "cog_category": "FR" }, "pscc": { "uniref50_id": "UniRef50_P32083", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P32083" ], "product": "Uncharacterized 13.1 kDa HIT-like protein in P37 5'region" }, "genes": [ "hinT" ], "id": "LMJBGEANGM_813", "locus": "GCA013368015_00804" }, { "type": "cds", "sequence": "contig_1", "start": 463477, "stop": 463605, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTAATTTTGAGTCATTATATAAAACTGCAGTTAAATATATTAATGAACTAGGGTTTAAATTAAATGTTTCAGAAACAAATAAATCCGTTTATGATATATTGAAAGTTGAAGGTCTGGTATAA", "aa": "MTANFESLYKTAVKYINELGFKLNVSETNKSVYDILKVEGLV", "aa_hexdigest": "bbfeebaf8b194e99c537736287b7ff2e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4770.4540000000015, "isoelectric_point": 6.0701997756958 }, "id": "LMJBGEANGM_814", "locus": "GCA013368015_00805" }, { "type": "cds", "sequence": "contig_1", "start": 463645, "stop": 464340, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGCGAAGTATTAAAATCATTATTGGCATATAAAGGCATTGTTAAAAATTCAAATACGTCAGGCGATTTAGGTGGCAATTTAGATAGTATAAAAAAAGATTTAAGTTCAGTATATGGATTTGTAGATCCATATAGCAAAAAAGTTTATAGTCAAGAAAGCTTTTTACTTGCAAAAATTCTTGCCAAAGAAATAAACCATCCAAAGGCAAAAGCAACCGAAACATTGCAATCTAAAGCTTCAAAGGGCGAATTAAAATCATTTGATTACAAAGATTACGAATTTGAAGGTTTAACAAAAGATAGCAAGGATAATTATCAATATACAAAGACAATAACTCTTGATAACAAGCAATATACATTGAGATTTAGCCAAAAAGGTTCAATATCATTTGACGGAAACTTTTTAACAATTCCTATGAATTTAACAGTTGATGGTTTAGGAAAAAGAAACTTCTATGAATTCAATGCTAAACTAGAATACAATAAAAATACAAAGAAATTTGAGTCAATAAAACAAGATGTTGCATACAATTTAAAACAAAATCCTCAAAAAATAAATGTTACAAAAGACAATTCAATTACTGCCCAATATGTAGTTAAAATTTCACCATTATATTTAACTAAGAAAGTTAAAGATTATAACGGCAAAGAAGTTACAAAACAAGTTTTAACATTTGATGAAACTCCATGA", "aa": "MEGEVLKSLLAYKGIVKNSNTSGDLGGNLDSIKKDLSSVYGFVDPYSKKVYSQESFLLAKILAKEINHPKAKATETLQSKASKGELKSFDYKDYEFEGLTKDSKDNYQYTKTITLDNKQYTLRFSQKGSISFDGNFLTIPMNLTVDGLGKRNFYEFNAKLEYNKNTKKFESIKQDVAYNLKQNPQKINVTKDNSITAQYVVKISPLYLTKKVKDYNGKEVTKQVLTFDETP", "aa_hexdigest": "6bb2ecac2abfe43c89b1b9cba569f601", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26268.485500000017, "isoelectric_point": 9.279982185363771 }, "id": "LMJBGEANGM_815", "locus": "GCA013368015_00806" }, { "type": "cds", "sequence": "contig_1", "start": 464503, "stop": 464859, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATCAATGCAAAGATTGATACAACTAAATATAATCCTGCTGCATTTTCAATACCAAGTGAACAAGAATTTAAATTTATTTTTAAACACTCAAATCAAATCGCGGCAAACATTAGATTAGAACTTCAAAAAATGTTGCTTGCTAAACTTTATTTATTAAAAAATAGACCAGAACTTAAAAAAATAGCAAATGATAGCAACGGTCTAGACAAAGCACAAGTTGCATTACACAACAAAATGTCTAAGAAAGATGCTCCTATTAATGAAAAAGAATTATACGAAGCATTGAATTTTGCAGATGATTCATTGTATTTAATGAAATATCTAGTTGAAAATCCAATTATTGAAAATTGA", "aa": "MFINAKIDTTKYNPAAFSIPSEQEFKFIFKHSNQIAANIRLELQKMLLAKLYLLKNRPELKKIANDSNGLDKAQVALHNKMSKKDAPINEKELYEALNFADDSLYLMKYLVENPIIEN", "aa_hexdigest": "702d36993df8f55730155617ae261748", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13632.67669999998, "isoelectric_point": 8.730904960632323 }, "id": "LMJBGEANGM_816", "locus": "GCA013368015_00807" }, { "type": "cds", "sequence": "contig_1", "start": 465061, "stop": 465225, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAAAAATTAGATTATTAACAGCTATTGCAATGCCACTTGCGGTTCTTCCTTTGATTGCTAGTTCATGTAAAAAAGAAAAGGAAGATTCTCAACAAAATTCAGGGTACCAAAAACGTGTTTTAAAAGATTCATTAACAAAAAATAGGGTTTTAACTTGA", "aa": "MTKKIRLLTAIAMPLAVLPLIASSCKKEKEDSQQNSGYQKRVLKDSLTKNRVLT", "aa_hexdigest": "96c489145654e415ec9ec61e1a5af3c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6059.176500000003, "isoelectric_point": 10.103952598571777 }, "id": "LMJBGEANGM_817", "locus": "GCA013368015_00808" }, { "type": "cds", "sequence": "contig_1", "start": 465237, "stop": 466361, "strand": "-", "frame": 1, "gene": null, "product": "HinT-interacting membrane complex protein P80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "nt": "TTGAATGAAAGTGATGACAAGGTTTCAATTAAACAAGGCATTGATGCCATTTCAGCATTTAAAGGTATATCTTCACTTCTTGAAACAAATGAAATTACTAAAAAACAAGAAATTGCGGCAAAAAATGATAGCAACCTTTTGAAATATGTTTCAAGCGATAGCAAGTCAGCTGATAAATACGGTTTCGACGGCTTTAAAAGTGTATTGAATAGTTTAAAAGAAAAAGATCCTAATAAGTATATGAATCTTCTTTCAATCTTAATGGGAGATGCTGACAAAGATAAAAGCATTTTTAAAGTTGAAGATAAAAATGATTTGTTTAAAAAATTAAAAGAAAACTTCAAAAAGATCTTTGGCGATGCTACATTTATTGATCATGAAAAGAAAACAAAGAAATTTGCTGATATTTTTAATGAAGGAACTGAAAGTGTTGACAAAGAAAACTTCTCTGAAAAATACACTAGATACAATGCAGCTATTGAAAAATTTATCAACGATTTAAGTGAAAAAGATTTTAATAAATCTTTTGGCTTGGCATTTCGAGATGCATTTAGTGATGAAAAAAGTGGATATAAGGTTTCAACATTAATTAAAAGTAATGGAAATTACATTCAAGTCAATGAAAGTGGAATTTTGATTCAAAATGTATTTTCATTAGACAAAGAAGAAGTTGTAACTAGATTGATAACAAACGATTTGTCAATACTATCTAAGGCTAATCATTCGGATACATTGAATACTAAATTATTTAATTTAGATTCAATATTTTCTGAAATTTTAACTCGTGACTATATGGTTAATGATTTATTACAACAAGATAATTTTAAAAATTATATTAAATCAAAAGAATATTTAAGTCTTGAAGGAACAAAACGTAAATTCAATGATCAAGATATTTTAAATGCAATCAACTATGAAAAATTAATTCGTGAAACAAGCAAATACGTTTTAATTAATGGCAAAGCAAACGAAATAAAACAATTTATAATTAAAAAATTTAATAGTAATTTATACAAAGATTATGTGTTCAAAGACAATAAGTTTATTCTATCGCCCCACGAAGACAAAGAGATCTTATCTTATTTATTCACAACAATTGTAAATTACATAAATACTAAAAATTAA", "aa": "MNESDDKVSIKQGIDAISAFKGISSLLETNEITKKQEIAAKNDSNLLKYVSSDSKSADKYGFDGFKSVLNSLKEKDPNKYMNLLSILMGDADKDKSIFKVEDKNDLFKKLKENFKKIFGDATFIDHEKKTKKFADIFNEGTESVDKENFSEKYTRYNAAIEKFINDLSEKDFNKSFGLAFRDAFSDEKSGYKVSTLIKSNGNYIQVNESGILIQNVFSLDKEEVVTRLITNDLSILSKANHSDTLNTKLFNLDSIFSEILTRDYMVNDLLQQDNFKNYIKSKEYLSLEGTKRKFNDQDILNAINYEKLIRETSKYVLINGKANEIKQFIIKKFNSNLYKDYVFKDNKFILSPHEDKEILSYLFTTIVNYINTKN", "aa_hexdigest": "cbb7b779e8b54168cb0953ab63de4ddc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDBC09", "query_cov": 0.8155080213903744, "subject_cov": 0.9967320261437909, "identity": 0.525, "score": 293.0, "evalue": 2.44e-94, "valid": false, "product": "HinT-interacting membrane complex protein P80", "uniref50_id": "UniRef50_UPI0021BDBC09" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BDBC09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "product": "HinT-interacting membrane complex protein P80" }, "genes": [], "id": "LMJBGEANGM_818", "locus": "GCA013368015_00809" }, { "type": "cds", "sequence": "contig_1", "start": 467037, "stop": 467159, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTATGGTGATATTAATAAAATCCATGAAATAGCAAATTCATCAAAACATGCTGCTGAAGGTGTTGAAAAGCAATTAATTAAATACCTTTATGAAAAAGAATATGAAGGTTCATTGTGA", "aa": "MNYGDINKIHEIANSSKHAAEGVEKQLIKYLYEKEYEGSL", "aa_hexdigest": "fc4f3f946ea3a21e1f35b177f91b4cf9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4614.1062999999995, "isoelectric_point": 5.503799247741698 }, "id": "LMJBGEANGM_819", "locus": "GCA013368015_00810" }, { "type": "cds", "sequence": "contig_1", "start": 467479, "stop": 467892, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5", "UniRef:UniRef90_UPI001F2617A5" ], "nt": "ATGAATAAATTACTTTTAAATAAGGATAGAATATTGCTTGAATTTATTGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTGAGCTTGTATGGCGCTATGTCAAAAGAACATTCTTGGAATAATTTAGATCACCATGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTGCATTCTTTGTATCACGACTTACAAATAAAATTTGTTGATGATAACAAAAATATATTTGAAAACCAACCGTTTTTGAATATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATTGCAATCAAGCTAGATCGTTTATTGGCCACTATTTAACAATGACAAATAGCAACGATTGA", "aa": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGAMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTNIANIKKYLKHCNQARSFIGHYLTMTNSND", "aa_hexdigest": "709e081c4eebd9b2c9a12b2ef78bbb1b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 467479, "stop": 468004, "observations": { "insertion": [ 467980 ], "mutation": [ 467890 ], "stop": [ 467890 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2617A5", "query_cov": 1.0, "subject_cov": 0.7873563218390804, "identity": 0.985, "score": 278.0, "evalue": 1.63e-94, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGTMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTSIANIKKYLKHCNQARSFIGHYLTMTNSNDWKTKNNIIKSHFLSMFYKPITYKEVANLLIKMITKVF" }, "paralog": false, "identity": 0.9770114942528736, "score": 346.0, "evalue": 1.66e-123, "description": "Internal stop codon at 467890. Frameshift due to insertion around 467980. Nonsense mutation around 467890.", "psc": { "uniref90_id": "UniRef90_UPI001F2617A5", "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2617A5", "UniRef:UniRef50_UPI001F2617A5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16338.62059999998, "isoelectric_point": 7.045727348327636 }, "id": "LMJBGEANGM_820", "locus": "GCA013368015_00811" }, { "type": "cds", "sequence": "contig_1", "start": 468046, "stop": 468225, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD", "UniRef:UniRef90_UPI001F176BBD" ], "nt": "TTGCCAAATATTAAATTTGAAACAAAAAAAGTAATTATAAACAATATTGAAGTTGATGTTTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATTCCGTTCCACAAAGAAAGATAGAATATATTAACCCCATTGAATATAAAAAAATATTTAGCTAA", "aa": "MPNIKFETKKVIINNIEVDVLVEFDQYLKNVYEVEYFEHSVPQRKIEYINPIEYKKIFS", "aa_hexdigest": "84b31e13c5316cb7c8a24db57c47004b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F176BBD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.966, "score": 115.0, "evalue": 8e-33, "valid": true, "uniref50_id": "UniRef50_UPI001F176BBD" }, "pscc": { "uniref50_id": "UniRef50_UPI001F176BBD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7226.258899999999, "isoelectric_point": 5.270873069763183 }, "id": "LMJBGEANGM_821", "locus": "GCA013368015_00812" }, { "type": "cds", "sequence": "contig_1", "start": 468383, "stop": 469327, "strand": "-", "frame": 2, "gene": "lolD", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1136", "COG:M", "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2", "UniRef:UniRef90_D1J8E0" ], "nt": "ATGGCAAAAGAAATAAAAGAAAACAAAAAAGAAATTAAAGCTAAAACAAATAAAATTTCTAAGAATAAAAATTTTGTTGATGAACAAACATTGCAAGATTTGAACTTGATAAATATTTCTAAGCAAAAAAAGCCATTTGAAATTAAAGATGAAGGCATTGCAATTAAGGTTTTAGATAATGCAACTTTAAAGAAATATAGACTTGCTAATGACAAACCAAGAAAAAAAGACGAGTATGCTAAATTGCAAAATTCTAACGATAACATTGTTGAAGTTGATAATATTAAAAAAACGTATTTATCTGGCAATGTCGCAACTGAAGTTTTAAAAGGCATAACTTTTTCAATTAAAAAAGGAGAAATTGTTGTTCTATATGGAAAGTCTGGGTCAGGTAAATCAACATTATTAAACATAATTAGTGCTCTTGACCGGCCTACCTCTGGCAATGTAATTGTAAACAACATAAATTTACCTTATTTATCTGATGCAAAGCAAACTTTATTTAGAAGAGAAAACATTTCGTTCATTTTTCAAAATTATAATTTACTTCAAAATTTGAATAGTTATGACAATGTTGAGACTGGTTCATATTTGCAAAGAGATAAGAACAAGCGTTTAAATATTAAAAAATTATTTGAGGATTTTGATCTTTCTGAATGTATGTATAAATATCCTTCTCAAATGTCTGGTGGTCAACAACAAAGAGTGTCAATTTTAAGAGCCATTGCAAAAAATTCCGAAATTCTTGTAGCTGATGAACCAACAGGAGCATTGGACGAAAAAACAGGAATTATTGTATTGAAAAATTTGCAACAAATCAATTTAAAATATGGCACAACCATTATCATTGTTTCCCACGACCCTGACGTTGCCCAAATTGCTGATAAAGTTATATACTTGGAGTTGGGATTAATTAAAGAAGTAAAACTTCAAGAAAGAAAATGA", "aa": "MAKEIKENKKEIKAKTNKISKNKNFVDEQTLQDLNLINISKQKKPFEIKDEGIAIKVLDNATLKKYRLANDKPRKKDEYAKLQNSNDNIVEVDNIKKTYLSGNVATEVLKGITFSIKKGEIVVLYGKSGSGKSTLLNIISALDRPTSGNVIVNNINLPYLSDAKQTLFRRENISFIFQNYNLLQNLNSYDNVETGSYLQRDKNKRLNIKKLFEDFDLSECMYKYPSQMSGGQQQRVSILRAIAKNSEILVADEPTGALDEKTGIIVLKNLQQINLKYGTTIIIVSHDPDVAQIADKVIYLELGLIKEVKLQERK", "aa_hexdigest": "662dd1bababf85e6d39c7f1fafb15a6c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8E0", "query_cov": 1.0, "subject_cov": 0.9721362229102167, "identity": 0.997, "score": 587.0, "evalue": 5.27e-211, "valid": true, "gene": "LolD", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A448ZXQ2", "cog_id": "COG1136", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "lolD" ], "id": "LMJBGEANGM_822", "locus": "GCA013368015_00813" }, { "type": "cds", "sequence": "contig_1", "start": 469377, "stop": 470057, "strand": "-", "frame": 1, "gene": null, "product": "FtsX-like permease family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702", "UniRef:UniRef90_UPI001F16D702" ], "nt": "ATGTTCATAAGCAATCCAAAATTGAAATATATTTCTGTATATGAACATAACATTCAATCATATAATGAAGCACATCCTCAGGCAAAATTAGATTATAAAACTCAATTAAAGAAATTACTTGGAAATCTAAACGATGAAAGCATACAAGAAATGATAGACTCAGACAGCCCATTAGAAAGTTATAAAATCTTAGTAAGAGAAACATTAACTAAATTCTATGGCACACAAGCATCTTCAATATATGGCAAGAATATAATGTATGGTGCATCATTTGATGTTAATAGTAAAGACATTGAAGCTGGATTTATTGAAGGAATTTCTTCAACAGTTAATACCATATTGCTGGCATTTATAATAATTTCATTGATAATTTCAATAGTTATATTAATTGTTATTACTAATATAATGATTGCTTCAAATGGAAGATCAATTGCTACATTTTCTATATTAGGATATACAAATAAAGAAAAAATTCTATTATTCTTCTTTAATTTTGTACCTGCAATTGTATTTGCTTGCTTACTAATGATTCCTGCAACTCTTGCAATAATAGCAATATTTAACTCGTTTATGATGGTAACTAGCCAGATTGTATTACCTTTGGTTTTACATATTTCAACAGTAATATTAAGTGCATTAATTTGCTTGGCAGTATTTATAGCAACATCAATTGCAACATGA", "aa": "MFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDESIQEMIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIAT", "aa_hexdigest": "58cf1df706f76b66348fa755e6bf67c6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 469329, "stop": 470192, "observations": { "mutation": [ 469379, 470075, 470126 ], "stop": [ 469379, 470075, 470126 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16D702", "query_cov": 1.0, "subject_cov": 0.7874564459930313, "identity": 0.991, "score": 384.0, "evalue": 5.57e-133, "gene_start": 1, "gene_end": 226, "reference_start": 46, "reference_end": 271, "reference_sequence": "MSIDKVPVQTIDTLTTYSATGFWGAASSYDVAEANDNSAWDFFKRMFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDENIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIATWNSLNKIKAVDALKGK" }, "paralog": false, "identity": 0.9790940766550522, "score": 512.0, "evalue": 5.31e-186, "description": "Internal stop codon at 469379, 470075, 470126. Nonsense mutation around 469379, 470075, 470126.", "psc": { "uniref90_id": "UniRef90_UPI001F16D702", "product": "FtsX-like permease family protein", "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16D702", "UniRef:UniRef50_UPI001F16D702" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702" ], "product": "FtsX-like permease family protein" } }, "id": "LMJBGEANGM_823", "locus": "GCA013368015_00814" }, { "type": "cds", "sequence": "contig_1", "start": 470124, "stop": 470627, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTGCAAGATAGTCGGGGCAATAATTTAACAAAATTATTACAAGACTATAATTACAATTTTGATAGTAATGACCCAATTCCTGTTGTTGTAAATGAAGTAACAGCAAGAAGCTATAGATTAAAAGTTAATTCCGTATTTGATGCCAAAATATTAAATCACGTTGATAGATTTGCATATCAAGCCTTCATGCAAAAAAGTCCAAGAGTTGATTATAAGTTTAAAGTAATTGGCATATCAGATACTTATATTAACTCTGAAATTAGTACAAGGAAGGATATTTTAGACCACATTTTAGGCTATGATACTCTTTCAAAAAGATTAAAAGATGCAAGAAAATACGAATTACAAAAAGCAATTAGTTTACACCCTGATTTAAAAGATGAATATATTAAAAAATTCAATAAAAAATATGATGCATTTAATGGTATTTTATCAATTGATAAAGTTCCAGTTCAAACTATTGATACATTAACAACTTATTCAGCAACAGGATTCTGA", "aa": "MRLQDSRGNNLTKLLQDYNYNFDSNDPIPVVVNEVTARSYRLKVNSVFDAKILNHVDRFAYQAFMQKSPRVDYKFKVIGISDTYINSEISTRKDILDHILGYDTLSKRLKDARKYELQKAISLHPDLKDEYIKKFNKKYDAFNGILSIDKVPVQTIDTLTTYSATGF", "aa_hexdigest": "3011ad45e3d00215dce907126648aaff", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19399.842900000003, "isoelectric_point": 9.220929145812985 }, "id": "LMJBGEANGM_824", "locus": "GCA013368015_00815" }, { "type": "cds", "sequence": "contig_1", "start": 470862, "stop": 471170, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGAAACACAAAGGGCAAGAGTAAACCAAATATTTAACCGCGTAAGTTCAATGATGCAATCAACTCAAAACTTAATAAATTTACGTAGATTGAAAAATGGTGGAGATTATAGAATAATAGATGAAGAAGATGTAAAAGATCCAACAAAAATAATCGCTGTAAAAGATATTAAAAAATTCTTTGATGACTATAATGCTAAACGGCCTGAAGATTTATCAAATAGAACAAGCTACTTCTTGTTTAGCGCTGGTAATTATTCAGAAGCAGCATCAGGAAATAGAAAACAATTTAGATTTGTTGAATGA", "aa": "MPETQRARVNQIFNRVSSMMQSTQNLINLRRLKNGGDYRIIDEEDVKDPTKIIAVKDIKKFFDDYNAKRPEDLSNRTSYFLFSAGNYSEAASGNRKQFRFVE", "aa_hexdigest": "6b08e1a730bc0aa42ee5b39d2731b77b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11945.299099999987, "isoelectric_point": 9.51716136932373 }, "id": "LMJBGEANGM_825", "locus": "GCA013368015_00816" }, { "type": "cds", "sequence": "contig_1", "start": 471189, "stop": 471575, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTCGTTATTCTTAGCATTTTCAACGACTTCATTAATTTCTATGTTCTTCTTATCTTCAAATAACGTGTTTAATAAAACTATTTCAAAGACATATAAAAATAGGCTATATAAATATAAATTAAATCTTGAAAGTCCAACAACAGAAGGTGGTCCTTATGTTACATACAATCAAAACGATATAAATGATTTATTGTATGTTCCTAATGATCTTGCAGGTGATTCATCAACCAATGGTAGTCAACTAGATTATGATAATCCTAATTTCTTAAGACCTGGAGCTTCATTTAATACTGATGTAATTCAAAGAAAATTTGCGCCTACTGTTATTACAAAATCATCGCTTGATTTATTAATGGATTTATCAGTTGAATTAAGTCCTTGA", "aa": "MFSLFLAFSTTSLISMFFLSSNNVFNKTISKTYKNRLYKYKLNLESPTTEGGPYVTYNQNDINDLLYVPNDLAGDSSTNGSQLDYDNPNFLRPGASFNTDVIQRKFAPTVITKSSLDLLMDLSVELSP", "aa_hexdigest": "81bfa1e11e916d49b86f73582905571f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.00049999998, "isoelectric_point": 4.882492256164551 }, "id": "LMJBGEANGM_826", "locus": "GCA013368015_00817" }, { "type": "cds", "sequence": "contig_1", "start": 471579, "stop": 471764, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTGTTTCACTAGTTATTTATATAATTACTAGAGTTATTGTGAGAAATAAACCTACAGAATTAATGAGTGGGTTAACTGAAGTTAGTGTTGGTAATTTTGCCCAAAGAGTTTCTGCTATGTTTAGAAAAATGCCAGTTAAAGCAAGATTTATTGCCTCGATGGCATTGAATAATTTCTGA", "aa": "MIVVSLVIYIITRVIVRNKPTELMSGLTEVSVGNFAQRVSAMFRKMPVKARFIASMALNNF", "aa_hexdigest": "ec87560bdd6cd360c1edb573737bef73", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6860.252400000002, "isoelectric_point": 11.466427421569826 }, "id": "LMJBGEANGM_827", "locus": "GCA013368015_00818" }, { "type": "cds", "sequence": "contig_1", "start": 471915, "stop": 473516, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGGTCTTTACAGCATAGGAATATCAAAGGAAATTAAAGATATAGTATTAAATTACAAAAAAGTAATTAACCCAATTCCTAGTCTTGGAATTTTAGGCTCGGACGATGACTATTCTGCTAAATTATTTAAAATGCCTTTTGCTCACGCACATACAGCAAAAGCAACAAAACAAATTATGGATTTAATGAATAATTCAGCAGTATTTAAAAATTTATTTGACCAATTATCTAAGAATCATGTTTCTAATAAGGGCATTGAAGCAATTAAAGAGATATTAAAAAATAATATGAATGAAATAACCTACAATTTTGGTTATATGGCCTCTTCAAATCAAATGCCAACTCATTATCTTCAAGCCTTAAAATTATTTTTACAATCATTTATTAATGTCAATGATCAAAACCAAAAAATTAAACCGTTAATAAATGATGAATATACTTTTAGCTTAGTATATAAGGATGCAATTGCAGCTTCAAAAACCGCCTCAATAATTTCGTTGTTAAACGTACCAAGAAATATTCTAAATGGTTTCAATATTTTAAGTTTCCCACAAATATTGTTATATTACGTATTAAGTCCAAACCCAGATAGTGGAAATATTGCTTATATAATCAAAAAATTCTTATCAAATATTGATGAAACTAATGCAACAATGTTGAAAAAACAAATCGAAGGTGCAACTCAAAAATTCAATTTTGTTGAAAATGTTGTTACTTCAAAAAGCGATAGCAGAATTGATTTAGATATTTCTAAATTCAATTATATGTTTAACCATTTATTAAAAACACCCGATAACAAAGATATTAGCTTTTTTGGAATAAATATTACCAAAACTGTTAAGGAAGTATTTTCAAAAATAATAGAACCAAGCACAGTTTATAATTTAATTGCATATTCCGAAGCTGCTTCATATTTAGCCAAGGTAAATTATGGATATTTAAACAAAAATAAAAAAGAAGTATATAATGGCGATATTTCAAAATATCTAAATAATCCATTTGAAATGCAACAATTCATTGCTTCATTAGATGATAAATATAAGATTAAAGTAAATACTCAAGAATATTTGATAACAGGTATTGATAGTACCGCTGATTATTTATATCCTGTAATTAATGAAGAAAACTTACAAGTTGATACAAAAACTCAATCTGTTGTTTATGTTAATTCAAGAGGTTTTGACAGAATTAGGTCGGCATATCCTACATTTGCCTTAAAGAGTTATGCATTAGTAAAATCCCCTGTTGACAAAAAAGGAAATTATTTAGCTGGCAAAGATCCTAAAAACTTAAAGAAAGAACTTCAAAGAATTGTTTCATCAATTTCTCCAAATTCAAAAAATAAAGTATTTTTAAAAGATGAACCAGATGCAATCAACCCTGAACGTTACATCAGAGTAAAAACAATAAGAACATTAGTAACATCAATTAAAAATGCAACACTTTATTTAATAACAATTTTGGCAATATTAGTAATTTTGATTGTTTACTTTATTATCAAAAGATATATTGAAGCAAGAAATAAGGTTGTTGGAATATTAAGAGCTCAAGGATATAAAACATCAGAAATCGCTTTATCATTCTGTGCTTTTGGTTGA", "aa": "MLGLYSIGISKEIKDIVLNYKKVINPIPSLGILGSDDDYSAKLFKMPFAHAHTAKATKQIMDLMNNSAVFKNLFDQLSKNHVSNKGIEAIKEILKNNMNEITYNFGYMASSNQMPTHYLQALKLFLQSFINVNDQNQKIKPLINDEYTFSLVYKDAIAASKTASIISLLNVPRNILNGFNILSFPQILLYYVLSPNPDSGNIAYIIKKFLSNIDETNATMLKKQIEGATQKFNFVENVVTSKSDSRIDLDISKFNYMFNHLLKTPDNKDISFFGINITKTVKEVFSKIIEPSTVYNLIAYSEAASYLAKVNYGYLNKNKKEVYNGDISKYLNNPFEMQQFIASLDDKYKIKVNTQEYLITGIDSTADYLYPVINEENLQVDTKTQSVVYVNSRGFDRIRSAYPTFALKSYALVKSPVDKKGNYLAGKDPKNLKKELQRIVSSISPNSKNKVFLKDEPDAINPERYIRVKTIRTLVTSIKNATLYLITILAILVILIVYFIIKRYIEARNKVVGILRAQGYKTSEIALSFCAFG", "aa_hexdigest": "c54cbf77554387006e0e49a62232c3e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 60492.270500000035, "isoelectric_point": 9.393704414367676 }, "id": "LMJBGEANGM_828", "locus": "GCA013368015_00819" }, { "type": "cds", "sequence": "contig_1", "start": 473649, "stop": 474443, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAACTAGTTAAACATGAACATTATCCAATTGTTGCAAATAATAAAATTTATAAAGATTTCAACACAGAAAGTTTAACTAGCTCAGATTATTTAACATCATTTTTTGGTGCAATAAGTTCAAACATAGATGGAAAAATAAATAGCGGAAAAATTTCCCAAATTCAAGAATCCGTTATTCAAATGTTTAATTTTTCAAATAAGACAAAGAGAATTTTGGGTAAATTGAATATAACTATTCCTGACTATGACAATAATAAAATTACTTTGTTTGATTTAATAAACATGTCAAAATTCTCATTCCCTCAATCTCAAAAGCAAACAAATGTATTGACTAATATTCCAATAAATCCTTTTGATGTTGATGATATTAAAAAAATATTATTAAAGGCAAAAGATGCAAAAATAAATAACAAAAAGTTGAAATTAACTCAAAACGAATTTGATGTATTAAAAAACAATGTTTTAGTTACCAAAGACGAATTTAATGACCTTGATTTAATAATTTCAAAATTAACTAAATATCATGAATATATAGACAAACTTGTAACATCAAACTCTCAATATAAGCAAAGCGGTATAAATTCGGCAGTTGAATTAAATGATTTAAAATCTTATTTGGATGTTGCATATTATTCAGCTTCATTTAATAATATAGCGCCTGAACATGCAAAAGATTTGGAAATTTTAAAAACTATTCATTCATTAATAAAAGACAATTTAGCAAAATTAATGTTGAATAATGATCCACAAAATGTTGCTTCTAATGTCTTATCTCTATATTCATTATGA", "aa": "MEQLVKHEHYPIVANNKIYKDFNTESLTSSDYLTSFFGAISSNIDGKINSGKISQIQESVIQMFNFSNKTKRILGKLNITIPDYDNNKITLFDLINMSKFSFPQSQKQTNVLTNIPINPFDVDDIKKILLKAKDAKINNKKLKLTQNEFDVLKNNVLVTKDEFNDLDLIISKLTKYHEYIDKLVTSNSQYKQSGINSAVELNDLKSYLDVAYYSASFNNIAPEHAKDLEILKTIHSLIKDNLAKLMLNNDPQNVASNVLSLYSL", "aa_hexdigest": "f3474509d1da9e225b0e6db4600a5d85", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30079.960100000048, "isoelectric_point": 7.763774299621583 }, "id": "LMJBGEANGM_829", "locus": "GCA013368015_00820" }, { "type": "cds", "sequence": "contig_1", "start": 474816, "stop": 475421, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATAAGTTTCGGGGCAATAAACAACAATTTAACTAACGAAATTATTCAAAATAAAACAATAAATGGTTTAGTTAATGTTATTAGAGACGCCTTATTTAATAGTGAAATTATTAACATGTTCAATAAAGACGATTTATTGAGAATCGTGGATGCAGCAAAACAAAGTGTTGTGGATAATGATTTCCATACATTGCTAAATATTGGTAAGGTAAATGGCACGATTTTACAAAAAGTTCTTTTAGATATTTTAAAATACATTATTAGACCAGTAGATAGAAGTAATAGTAACAATTTAAATATTAATAATGTTAATGTTAATGCTTTTGTTCAAAATGTATTCATTGGAATAATAAACTATTTAGAAAAAATGTACATGAATTCAGGAACCAATGACAATGAACGAAATCAATATTTAATGCAACAAGTTTACAACATAAGCAAACTATTAAATCTTAATAATCTATATTTGATTCCTTCTTTGAAATTAAGTTTATTTGACATAATTAATTTAGTTAAAAATAAATCAACAATTTTTGAAGTATTGAGAAATGTTATTCGCTCTATTGATTTTGAAAAATTTTCTTTAATTATTCAAGATTGA", "aa": "MPISFGAINNNLTNEIIQNKTINGLVNVIRDALFNSEIINMFNKDDLLRIVDAAKQSVVDNDFHTLLNIGKVNGTILQKVLLDILKYIIRPVDRSNSNNLNINNVNVNAFVQNVFIGIINYLEKMYMNSGTNDNERNQYLMQQVYNISKLLNLNNLYLIPSLKLSLFDIINLVKNKSTIFEVLRNVIRSIDFEKFSLIIQD", "aa_hexdigest": "1eb27169d3c1eca2b019bdeda0ed2da6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23064.41920000002, "isoelectric_point": 7.875409507751465 }, "id": "LMJBGEANGM_830", "locus": "GCA013368015_00821" }, { "type": "cds", "sequence": "contig_1", "start": 475566, "stop": 475745, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAACACAAAAATACCTTATGAAATCGATGGAAAAATATTGATGTTGACTGTTGCTAATGATGAACCCAAGACCGTTGGCTCTTCAATAGTTGGTGCAGTTGCAAGAATTAGTGATGAAAAATACATTTTATTAAAATATTCAACTATTGACGGATTTAGTGATAAAAAAGTTTGA", "aa": "MPNTKIPYEIDGKILMLTVANDEPKTVGSSIVGAVARISDEKYILLKYSTIDGFSDKKV", "aa_hexdigest": "be2686358a6ebaa3cc9d5e47637ab526", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6474.4519, "isoelectric_point": 6.099187660217283 }, "id": "LMJBGEANGM_831", "locus": "GCA013368015_00822" }, { "type": "cds", "sequence": "contig_1", "start": 475803, "stop": 476066, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGAATCCGTTGATGAAAAAACTACTAAGAAAAACGTTTTTCACTTTGTTAATGCAGGCGATGGAAACCAAAAAATTTATGGCGTAAAAAATAACGTTGGTAAATTATATAACGAAACTCTAAATCCTACATTACTAAATTCTTCTGTATCAGATAAAAATGTTAATGAATTTATTTTGAAACCCGATCCAAACGATCCAATGATCAAAAAAATTCCACCAGTTTATACAAAACAAATAATTAAATATATTTTTGAGCAATGA", "aa": "MESVDEKTTKKNVFHFVNAGDGNQKIYGVKNNVGKLYNETLNPTLLNSSVSDKNVNEFILKPDPNDPMIKKIPPVYTKQIIKYIFEQ", "aa_hexdigest": "e2605dad3ab6c25b5f7534af460befb2", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9944.267599999994, "isoelectric_point": 8.831346702575683 }, "id": "LMJBGEANGM_832", "locus": "GCA013368015_00823" }, { "type": "cds", "sequence": "contig_1", "start": 476625, "stop": 476906, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACACTATTGATGGTAAAGATGTAATGAAGATGCTTGGTTTTAAAGAAATTTCAGAAAACCATTATGAATTTGATGCATCTCTTTATAATAAAAACTCTGATTTAAAAATCGAAAATTCGGATTTTACAAGTCAAAAATCAAAATTTATTAATGAAGACTTTAATATTTCAAGAACTTTTAAACTTCAAAATTTTTTAAGCAATAAAAGTATTGAAAAAGATGTTAATGAATTTACAAAACTAGCAGCAAATAAAATATATCAAATTCCAAATGAATGA", "aa": "MHTIDGKDVMKMLGFKEISENHYEFDASLYNKNSDLKIENSDFTSQKSKFINEDFNISRTFKLQNFLSNKSIEKDVNEFTKLAANKIYQIPNE", "aa_hexdigest": "cc67ce97dd05b14625ea52c2116d74da", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10920.116399999988, "isoelectric_point": 5.6785791397094725 }, "id": "LMJBGEANGM_833", "locus": "GCA013368015_00824" }, { "type": "cds", "sequence": "contig_1", "start": 476922, "stop": 477704, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGACTTTTTAAAGAGGTTTTCAAATCATTGGCAAGAAATAAAGTTACCTTGATTTGTTTAACAATTTTAATTTTCTTAACAACATTTTTATTTACTTTGTTAAATGATGTTACTACTAGCTATTCATCTACGATTAATTCGTATGAAAAAGTTTCAAAATTGCATGATGCAACTGTTGATTTTGACGTTAACCCATCAGGTGTGATACCAAAATCAGGTTATAACCAAATAGGATCTGATAATCAATCAATTGTTGATAAACCTGTTGTTTTTGAGTCTTCTAATAATGGAAGTGATATTTCATATTCAATAGATCTACCAAAAGATCAAAGAAATTATGTAGGTTTAAAAAATACTTTTAATAATTTAAATTATGATAATGAAAACCATTTTATAAATACTTATGATTTATTAAATTTATTATATTCTAGCAAGGAATCTATAAGTGATGTAGACGTTGAAATTTTAAAATCCAACCCTGATTTTTCTAAAATTAGGAATTTTAGTTTTTCAGGTCACGATAGGGAATTTAAACTTTATATAAAAGATAGCAAAGGAAATATAAAACCGTTAACTAAAAGATATAATTTGACAAAAAATGATGACATTACTTTTTTAAATGAAGTTAGATTAAAAAATATAGCTTCTATTGCATATGGTCCAAGAGGTTCACTAAACGATCAAAAAGTAGTTGATTATTTATACAATCCCTCTCCATTATTTCTAAATGTTAAAACTAAACAAGCATCATTTGATACTAAAGATTATGAAGCGTGA", "aa": "MRRLFKEVFKSLARNKVTLICLTILIFLTTFLFTLLNDVTTSYSSTINSYEKVSKLHDATVDFDVNPSGVIPKSGYNQIGSDNQSIVDKPVVFESSNNGSDISYSIDLPKDQRNYVGLKNTFNNLNYDNENHFINTYDLLNLLYSSKESISDVDVEILKSNPDFSKIRNFSFSGHDREFKLYIKDSKGNIKPLTKRYNLTKNDDITFLNEVRLKNIASIAYGPRGSLNDQKVVDYLYNPSPLFLNVKTKQASFDTKDYEA", "aa_hexdigest": "d873429e8f0701079966a9074e65496b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29719.08860000005, "isoelectric_point": 7.699773597717285 }, "id": "LMJBGEANGM_834", "locus": "GCA013368015_00825" }, { "type": "cds", "sequence": "contig_1", "start": 477889, "stop": 478227, "strand": "-", "frame": 3, "gene": "rsmG", "product": "RsmG family class I SAM-dependent methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D54A", "UniRef:UniRef90_UPI002410D54A" ], "nt": "TTGGTTAAAAATGAGTTGAAATTGAAAAATATTGATATACAAAAAATTCGTGCTGAAGATTCAAATCAAATAGAATGTTTTGATTTTATTAGTGCAAGAGCGGTGAGTGAATTAAAAAATTTATTAGAAATTTCACACCATCTTTTAAAAATTAATGGAATTGCTTGCTTTTTAAAATCAAATAAATATCAGCAAGAAATAGCTAATTCAAACTTTATAGTTAACAAATTAAATATTAATTTAAAAACAAGGATTTTGCCAATATATTTCAATATTGAAAATGTTTTAATTTTTCACCAAAAATTAATAAAAACAGCCTCAGGATTTCCTAGAAAATGA", "aa": "MVKNELKLKNIDIQKIRAEDSNQIECFDFISARAVSELKNLLEISHHLLKINGIACFLKSNKYQQEIANSNFIVNKLNINLKTRILPIYFNIENVLIFHQKLIKTASGFPRK", "aa_hexdigest": "8dd8912789829373f67b46d2acf1ba05", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI002410D54A", "query_cov": 1.0, "subject_cov": 0.9032258064516129, "identity": 1.0, "score": 219.0, "evalue": 3.28e-72, "valid": true, "product": "RsmG family class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_UPI002410D54A" }, "pscc": { "uniref50_id": "UniRef50_UPI002410D54A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D54A" ], "product": "RsmG family class I SAM-dependent methyltransferase" }, "genes": [ "rsmG" ], "id": "LMJBGEANGM_835", "locus": "GCA013368015_00826" }, { "type": "cds", "sequence": "contig_1", "start": 478269, "stop": 478568, "strand": "-", "frame": 1, "gene": "rsmG", "product": "RsmG family class I SAM-dependent methyltransferase", "db_xrefs": [ "RefSeq:WP_241863042.1", "SO:0001217", "UniParc:UPI001F51865A", "UniRef:UniRef100_UPI001F51865A", "UniRef:UniRef50_UPI001F51865A", "UniRef:UniRef90_UPI001F51865A" ], "nt": "ATGCTTACGGAAAAAGAAAGTAATAATTTAATATTGCAAAGTCTACCACAATGTAGCAAAGAAGTCTTAAAAAAATTATATGAATATTATCAATTAATAGAACAAGAAAATAGCAAATATAATTTAACAGGTTTCTATAACGAAAAGCTTTTAAAAGAAGCAATAATTGAATCAATTTTAATTTTTTTAGACATTGAAAAAAATATTTGTAATCTAACTAACAAATCTGTACTCGACATTGGCTCGGGCGCAGGCTTCCTATCCTTCCATATTTCATATATAAACAAGATTTTTCTTTAA", "aa": "MLTEKESNNLILQSLPQCSKEVLKKLYEYYQLIEQENSKYNLTGFYNEKLLKEAIIESILIFLDIEKNICNLTNKSVLDIGSGAGFLSFHISYINKIFL", "aa_hexdigest": "c4bd1e8a3b5228d1607c20fbb8e09b21", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI001F51865A", "ncbi_nrp_id": "WP_241863042.1", "uniref100_id": "UniRef100_UPI001F51865A", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F51865A", "RefSeq:WP_241863042.1", "UniRef:UniRef100_UPI001F51865A" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F51865A", "uniref90_id": "UniRef90_UPI001F51865A" }, "psc": { "uniref90_id": "UniRef90_UPI001F51865A", "product": "RsmG family class I SAM-dependent methyltransferase", "uniref50_id": "UniRef50_UPI001F51865A" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51865A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51865A" ], "product": "RsmG family class I SAM-dependent methyltransferase" }, "genes": [ "rsmG" ], "id": "LMJBGEANGM_836", "locus": "GCA013368015_00827" }, { "type": "cds", "sequence": "contig_1", "start": 478546, "stop": 479517, "strand": "-", "frame": 3, "gene": "prsA", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_036439353.1", "SO:0001217", "UniParc:UPI000512F627", "UniRef:UniRef100_D1J8E3", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_D1J8E3" ], "nt": "ATGAAAGCAAAAAAAGAAGATAATGTTGTAATTTTTGGCATGGAAAATTCCCAAAAATTAGCGCAAGAAATTGGAAAGTATTTAAATAAAGAAATATTGCCAGTTAAAACTACAGTATTTGCAGATGGAGAATGCCTTTTAAAATCAGAAACAACTGTTAGAAATAAGGATGTTTACATTATTTGCAATACCTCAAAACCAGTAAACGATAACATAATGTCATTGCTAATTTTAATCGATTCATTAAGACGTTCATCAGCAAAGAGCATTAATTGCGTTATGACCTACTATGGATATGCAAGACAAGATAGAAAAGCAAGTGGAAGAGATCCAATATCAGCTGCACTAGTTGCTAAGATGTATGAAACAGCGGGTGCTGATAAAATAATTACTATTGACTTGCACAACGCTTCAATTCAAGGATTTTTCAACATTCCTGTTGATGATTTAAGGGGACAATTTATTTTTTCAAATGAATTGCTAAACAGAAAAGAAAAACTAGTAATTGTATCACCAGACCACGGGGGTGCAGTTAGAGCAAGACAATTGTCAGAATTACTAAACAATGAAGAACAAATTGCAATTATTGACAAGCGTCGTACAGGACCTAATAAATGCGAAATTATGGGTGTTTTAGGTGATGTTAAAGATAAAAATGTTTTAATTTATGATGATATTATTGACACAGGTGGAACAATTATTCATGCAGCAGAAGCGTTAAAAGCAAACGGCGCAAAAAATATTATAATTGCAGCCACTCATGGTTTATTTTCAAAAGGATTTGAAATGTTTGAAAATAATGCAATGATTGATGAAGTTATTATTACTAATTCAATAGATCACAGCCATTTATCAAACTTTAAAAAATTAAGAGAATTATCTTTGGCTCAATTTTTAAGTATGGTAATCAATGCAAACATTGAAAGCAAATCTATAACAGAAGTATATAATGCTTACGGAAAAAGAAAGTAA", "aa": "MKAKKEDNVVIFGMENSQKLAQEIGKYLNKEILPVKTTVFADGECLLKSETTVRNKDVYIICNTSKPVNDNIMSLLILIDSLRRSSAKSINCVMTYYGYARQDRKASGRDPISAALVAKMYETAGADKIITIDLHNASIQGFFNIPVDDLRGQFIFSNELLNRKEKLVIVSPDHGGAVRARQLSELLNNEEQIAIIDKRRTGPNKCEIMGVLGDVKDKNVLIYDDIIDTGGTIIHAAEALKANGAKNIIIAATHGLFSKGFEMFENNAMIDEVIITNSIDHSHLSNFKKLRELSLAQFLSMVINANIESKSITEVYNAYGKRK", "aa_hexdigest": "f10c33be78cd5d79c9826892174e7740", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512F627", "ncbi_nrp_id": "WP_036439353.1", "uniref100_id": "UniRef100_D1J8E3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512F627", "RefSeq:WP_036439353.1", "UniRef:UniRef100_D1J8E3" ] }, "ips": { "uniref100_id": "UniRef100_D1J8E3", "uniref90_id": "UniRef90_D1J8E3" }, "psc": { "uniref90_id": "UniRef90_D1J8E3", "gene": "PrsA", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prsA" ], "id": "LMJBGEANGM_837", "locus": "GCA013368015_00828" }, { "type": "cds", "sequence": "contig_1", "start": 479711, "stop": 479899, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTCAAACCCTTGTAATTCACTTTTTGCGTACTGATAAAACACCATTTATTAAATCAAATGCAACGACCTCGTTATTTATTTCAACATTAATCGGATGTGCAATAATTAATGCCGTGCCATATATACCTGGAATTAATAAAGGTTTAAGTCTAATGCCATTGCCAGGAATTTATTATGCTTGA", "aa": "MYTQTLVIHFLRTDKTPFIKSNATTSLFISTLIGCAIINAVPYIPGINKGLSLMPLPGIYYA", "aa_hexdigest": "5bb3a49d8d55cdb133e821f609318a28", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6804.0436, "isoelectric_point": 9.360567665100099 }, "id": "LMJBGEANGM_838", "locus": "GCA013368015_00829" }, { "type": "cds", "sequence": "contig_1", "start": 480182, "stop": 481486, "strand": "-", "frame": 2, "gene": null, "product": "Magnesium-transporting ATPase, P-type 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "nt": "TTGTTTGGTATATCAATTGCTGTTGGTTTAACACCGGAAATGCTTCCAATGATAGTGACAGCAAGTTTATCTAAAGGTGCAGTTTCGCTAGGTAAGGCCAAAACAATAATTAAAAATATTAATTCAATTCAAAATTTTGGTTCAATGGACATTTTATGTACAGATAAAACTGGAACATTAACACAAGATGAAGTAATTATGGAACGTCATATGGATGTTACCGGCAAAGAAGATTTTCATGTTTTAAAATATGCTTTTATTAATTCATATTATCAAACAGGATTGAAAAATTTACTTGATAAATCAATAATTAATAAGACTTTTGAAATGGCGTCAAAAAACAAAGAACTTGAGGATTTTCAAAGATTTTATGAAAAAATTGACGAAATTCCTTTTGATTTCAAAAGAAAAAGAATGTCTGTTTTAGTTAAATCATTAAAACAAAATAAAGTAACCTTAATTACCAAGGGTGCTGTTGAAGAAATTGTTAGTGTATGTAAAAATATTTACATTGATGGGGAAGTTAAGCCTCTTGATAAAAAAACAATAGATAAGGTTCTAAGAAGAGTTGAAGAATTCAATTCAGAAGGACTAAGAGTTATTGCAATCGCTTCTAAAAAATCAACAATTAATGCAGTTGGAAAAATATCGGTAAATGATGAATGCGATATGACTTTGATTGGATATTTAACATTTCTTGATCCTCCTAAAGATTCTGCTGCTGCTGCAATTGAAAAATTGCATGAATATGGAGTTGATGTAAAAATATTAACGGGAGATAGTGCACTTGTAACAAAAGCAGTATGTGCCCAAGTAGGAATAAAAAATAATAGAATTTTACTAGGCAAAGAAGTTGAAGAATTAAGCGATGCTGAACTTAAAATTGAAGTTGAAAAAGTTCAAATTTTTGCAAAATTAAGCCCTGACCAAAAGGCAAGAATTATTTCAATATTGCGTGATAATGGTCATGTTGTTGGCTATATGGGTGACGGAATTAATGATGCACCCGCTATGAAATCAGCCGATGTTTCAATATCAGTTGATACTGCAGTTGATATTGCAAAAGAATCAGCTAACATAATTCTTTTAGAAAAAGACCTAAACGTTTTGGCTAAAGGAATTGTTGAAGGTAGAAAAACACATGCCAATATTAACAAATACATAAAAATGACTGCATCTTCAAACTTTGGAAATATATTTAGTATTTTAATTGCTTCATTTTTAATTCCTTTTGTTCCATTAAACCCTGTACAAATAATATTATTGAATTTGGTATATGATGTTTGCTGTGCAACAATGCCATGA", "aa": "MFGISIAVGLTPEMLPMIVTASLSKGAVSLGKAKTIIKNINSIQNFGSMDILCTDKTGTLTQDEVIMERHMDVTGKEDFHVLKYAFINSYYQTGLKNLLDKSIINKTFEMASKNKELEDFQRFYEKIDEIPFDFKRKRMSVLVKSLKQNKVTLITKGAVEEIVSVCKNIYIDGEVKPLDKKTIDKVLRRVEEFNSEGLRVIAIASKKSTINAVGKISVNDECDMTLIGYLTFLDPPKDSAAAAIEKLHEYGVDVKILTGDSALVTKAVCAQVGIKNNRILLGKEVEELSDAELKIEVEKVQIFAKLSPDQKARIISILRDNGHVVGYMGDGINDAPAMKSADVSISVDTAVDIAKESANIILLEKDLNVLAKGIVEGRKTHANINKYIKMTASSNFGNIFSILIASFLIPFVPLNPVQIILLNLVYDVCCATMP", "aa_hexdigest": "5b437bb35f2803d66af8e657cae419d2", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0001C17514", "query_cov": 0.9976958525345622, "subject_cov": 0.9039665970772442, "identity": 0.527, "score": 445.0, "evalue": 1.15e-150, "valid": false, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A2X4XS16", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2X4XS16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "product": "Magnesium-transporting ATPase, P-type 1" }, "genes": [], "id": "LMJBGEANGM_839", "locus": "GCA013368015_00830" }, { "type": "cds", "sequence": "contig_1", "start": 481499, "stop": 482059, "strand": "-", "frame": 2, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGGTTTTTTTAAGCGGAATTATTCACTTCGTTCAAGAACAACGTGGAGCATCAAGCGCTGATAAATTAGTTGAGACTGTTGAAACTACTTCGCTTGTTCAAAGGAATGGTATTATGAAGGAAATTCCAATGGATGAGATTGTAGTAGGCGATATTGTGTATTTAGCAGCTGGAGATATTATTCCGGCGGATGTAAGAATATTAGAAGCAAAAGACTTATTTATTTCTCAATCCTCATTAACCGGAGAAAGCGATGCTATTGAAAAATATAAGAACATCGACAGTTCTAAAGAATTTGAAGCAATAACTGATCGTAGTAATTTAGCTTTTATGGGAACCAATGTTATTTCAGGTTCTGCTAAAACTATTGTAATTGCAACCGGCAATGATACAATTATTGGACAAGTTGCTTCTAAAATCAATGAAAAACCAATACCAACCAACTTTGATAAAGGAATTAAAAAAATTTCAATAATGTTCACTATTATAATGGCTATTATTGTTCCTATAGTATTTTTAATTTTAGGAATTAAAGGTGTTATAGGTGGAGAAAATCAATGA", "aa": "MVFLSGIIHFVQEQRGASSADKLVETVETTSLVQRNGIMKEIPMDEIVVGDIVYLAAGDIIPADVRILEAKDLFISQSSLTGESDAIEKYKNIDSSKEFEAITDRSNLAFMGTNVISGSAKTIVIATGNDTIIGQVASKINEKPIPTNFDKGIKKISIMFTIIMAIIVPIVFLILGIKGVIGGENQ", "aa_hexdigest": "d7acc10c16c0472ebfbab860a65254d5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 0.9247311827956989, "subject_cov": 0.900523560209424, "identity": 0.535, "score": 157.0, "evalue": 2.1e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "LMJBGEANGM_840", "locus": "GCA013368015_00831" }, { "type": "cds", "sequence": "contig_1", "start": 482070, "stop": 482291, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACAGTCATTCCATCAATTCATGTTATCTTTTTACCTTTTGATGCTGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCGGCTTCAATTCTTTCTTCATCTTGA", "aa": "MMTVIPSIHVIFLPFDAGNIKSIIIETIANANKMMLKKLKNDLNKRFQTLSAPFFVILFKPYSSLAASILSSS", "aa_hexdigest": "5fd2be3efd989f8b301e8dd44131c75e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8130.694099999997, "isoelectric_point": 9.998095512390137 }, "id": "LMJBGEANGM_841", "locus": "GCA013368015_00832" }, { "type": "cds", "sequence": "contig_1", "start": 482210, "stop": 482437, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGATAGTTAATATAACAAAAAATAAACTTAAAAATTCTTCAAGAAAAGAAAAACAAATTAAGCAAATCAATACAAGGTTAGTTGAAGCTTCTAATTGTAGTCAAGAAGAATTATTTTTAAAATATCAAACCTCTCAAAACGGATTTCAAGATGAAGAAAGAATTGAAGCCGCAAGAGAAGAATATGGTTTAAATAAAATAACAAAAAAAGGCGCTGATAATGTTTGA", "aa": "MIVNITKNKLKNSSRKEKQIKQINTRLVEASNCSQEELFLKYQTSQNGFQDEERIEAAREEYGLNKITKKGADNV", "aa_hexdigest": "d642ef878636ebb810d5a26cc4121806", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 40, "aa_cov": 0.52, "hmm_cov": 0.975, "evalue": 7.816419734545715e-06, "score": 21.067995071411133, "start": 32, "stop": 70 } ], "seq_stats": { "molecular_weight": 8690.704999999993, "isoelectric_point": 9.130415534973146 }, "id": "LMJBGEANGM_842", "locus": "GCA013368015_00833" }, { "type": "cds", "sequence": "contig_1", "start": 482628, "stop": 482903, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTTGATACTTATTTTATTACAAAAAGTTGACATTATTTTAGCAGACGAACCTACCAATAGTCTTGATATAGCAAACGCAAGAGATGTTTTGGAATTATTGTCTTCAATATCACAAGAATACAAAAAAACAATTTTAGTAAATATTCACGACATTTCATTAATAAAAACTTATTTTAAGAAATACATTTATGTCAAAGATGGACAGATTATTGAAATTGGTGAAACAAAAAACTTAAATGAATTAAAAATAAAAGATTTATATAATTTTTAA", "aa": "MWLILILLQKVDIILADEPTNSLDIANARDVLELLSSISQEYKKTILVNIHDISLIKTYFKKYIYVKDGQIIEIGETKNLNELKIKDLYNF", "aa_hexdigest": "477e37b7292077b0aedcc44a98fda922", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10612.329799999989, "isoelectric_point": 5.278318977355956 }, "id": "LMJBGEANGM_843", "locus": "GCA013368015_00834" }, { "type": "cds", "sequence": "contig_1", "start": 483141, "stop": 483479, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAGGTTGATACAAGCCTCGCTGAAAAACATTGAGCAGAACACGCTTAATGTTTTTATTTTTGCAATATTTTGTTTTGTATTAATACATTCCAAATATAATTTAAATATGAAAAAAATTGAATTCATCAATGTTGATGCAAGTTATTATAAAAAAAGCCCAATAGTTTTAGAGAACATAAATTTTTCTATTGAAGAGGGTGAATTTATAGCGATAATAGGAAAATCCGGCGCTGGAAAATCAACAATTTTCAATGCTTTGTTGAGGCAATTATTTGTAAAAAAAGGCAATGTAAAAATTAATGATATTGATATTTATAGTGCTTCAAAAAAACAATGA", "aa": "MRLIQASLKNIEQNTLNVFIFAIFCFVLIHSKYNLNMKKIEFINVDASYYKKSPIVLENINFSIEEGEFIAIIGKSGAGKSTIFNALLRQLFVKKGNVKINDIDIYSASKKQ", "aa_hexdigest": "2c12a65860c9dfaa469fa5070030a422", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 51, "aa_cov": 0.45535714285714285, "hmm_cov": 1.0, "evalue": 2.1971394626464562e-14, "score": 49.78218078613281, "start": 57, "stop": 107 } ], "seq_stats": { "molecular_weight": 12766.853499999985, "isoelectric_point": 9.519997978210448 }, "id": "LMJBGEANGM_844", "locus": "GCA013368015_00835" }, { "type": "cds", "sequence": "contig_1", "start": 483486, "stop": 483812, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02866.23" ], "nt": "ATGGTTAAAAAAGAAGCATTTGAAATTATTGCCGGTAAAGGCATTACCAATTATGGTATTGGCGAAAACCTTGCTGAAATTACACAAGCAATTTTGTATGATAGACAAAGAATTTATTCTCTTGGAGTTCAATTGCCAAAGGAATATAAACATGCCGGAGTTTACTTTGGTTTACCAGTAATCTTGGGTAAGAATGGTTATACACACTTGCCAAAAATTAGACTTGATGAAGATGAACAACAACAATTTGATGAATATTCAAAAGAAATTAAAGAAACAGCAATTCAAGTATTAAAAAATTTGGATGTTGAAATTACTATTAAATAA", "aa": "MVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK", "aa_hexdigest": "0ff069a0685e97f7c251b507af1b4ee9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02866.23", "name": "Ldh_1_C", "length": 97, "aa_cov": 0.8611111111111112, "hmm_cov": 1.0, "evalue": 4.034026648990114e-15, "score": 51.76319122314453, "start": 2, "stop": 94 } ], "seq_stats": { "molecular_weight": 12343.04149999999, "isoelectric_point": 5.687104988098144 }, "id": "LMJBGEANGM_845", "locus": "GCA013368015_00836" }, { "type": "cds", "sequence": "contig_1", "start": 483861, "stop": 484454, "strand": "-", "frame": 1, "gene": "mdh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4", "UniRef:UniRef90_D1J8E6" ], "nt": "ATGACTAAAATAGGATTAATTGGCGTTGGTGCTGTTGGTAGTTCATATTTATATGCTGCATTAAATAAAAACATAGAAGCAGATTATGTTTTAATTGATAGTTTTCCACAAGTCGCAATTGCGCAAGCAAAGGATTTAAATGATGCTGCTTGTTCAATGGCAAATAATGGTTCAACATTCAAGGCTGGAGATTATTCAGATTTGAAAGATGCTGATATTGTTATTATAACTGCATCAATTAGACCAAAGGAAGGCGTTTTAAAAGATAGAATGGAATTATTGACCGATAATGCAAAACTATTGAGAAAGATCGGTCAAAGCATAAAAGATTCAGGATTTGCTGGTGTTACAATTATTGCTTCAAACCCTGTTGACATTATGGCAACAATTTATCAACAAGTAACCAAGTTTTCTCCAAATAAAGTTATTAGTTCGGGAACTATTCTAGAAACTGCCAGAATGAAAAAATTTCTTTCAGAAAAAATTGGAATTAAAGCACACTCAATTACTGGATTTGTTATTGGCGAACATGGAGCAAGATGCTTAATTCCATTTTCAAAAATAAGAATTGGTTTGGGTTCATTAAAAGATTGA", "aa": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFAGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKD", "aa_hexdigest": "2564416bb39feee5fdc00e457a316b01", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "mdh" ], "truncated": "5-prime", "pseudogene": { "start": 483558, "stop": 484454, "observations": { "mutation": [ 483833, 483863 ], "stop": [ 483833, 483863 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E6", "query_cov": 1.0, "subject_cov": 0.6118012422360248, "identity": 0.995, "score": 377.0, "evalue": 3.21e-130, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFDGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKDWLANGTITNEWLSQLNEMVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK" }, "paralog": false, "identity": 0.9899328859060402, "score": 571.0, "evalue": 4.67e-209, "description": "Internal stop codon at 483833, 483863. Nonsense mutation around 483833, 483863.", "psc": { "uniref90_id": "UniRef90_D1J8E6", "gene": "Mdh", "product": "L-lactate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z4LNL4", "cog_id": "COG0039", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E6", "UniRef:UniRef50_A0A2Z4LNL4", "COG:COG0039", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LNL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4" ], "product": "L-lactate dehydrogenase" } }, "id": "LMJBGEANGM_846", "locus": "GCA013368015_00837" }, { "type": "cds", "sequence": "contig_1", "start": 484591, "stop": 484926, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTCAGTGAGACCATTTCATCTATGAACAATAGGATTTATATTGAAACTTGCTTAGGGGGCGTCGATAACAAAACTTTGGCCTCAAATTACACCAACACTCCATTTAAGTGCTTTGATACTTGTTTAAAATTTCAAGAACCTATTATGGACTGCAAAATTTCACAAAATAACGAAAATGAGACGGATGGTTTGAATTATTTAAGCAATTTAACTTATAATAATTTATGTAATCTTGTAATGGAAACATTTATTGAAAACCATGTTAATGTATATAAAATACCTAACATTCAAATCATGATTGACAATTATAACGATAGTACATTGGGTTGA", "aa": "MLFSETISSMNNRIYIETCLGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLG", "aa_hexdigest": "44a004cbcfe51d4238e8609598e405ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12742.246099999986, "isoelectric_point": 4.352127647399903 }, "id": "LMJBGEANGM_847", "locus": "GCA013368015_00838" }, { "type": "cds", "sequence": "contig_1", "start": 484930, "stop": 485766, "strand": "-", "frame": 3, "gene": "pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "db_xrefs": [ "COG:COG0166", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8E7", "UniRef:UniRef90_D1J8E7" ], "nt": "ATGGAAAATAAGCAAATTATTGACATAAATTTAAAAAAAGCAATTAAAGAAAGTGCGCCAAACCGTTATTATGAGACGGTTCAAAAAATCTTGTTAGATATAAAAAACTCAAAGGTTAAAAATTCAAATTATTTAGGTGTATTCAATGTTTTTAAAAATATAAAGAAATCTGACTATGAAAAAATTCAAGAGATATCAAGCGAATTATTGTCAGATGGTATTGAATATTTAGTAATAATAGCAACTCAAACAATAATTAATCAAATTAAGGCGCTTGCTGATCTACACTTTGATTTAAGAAAAGAAAATGAATCAATTAAATTGGTTTATGTTAATGAAAATTTTAATAGTTTAGAGGTTGCCAAATTGCTTATTAAACTTCAAAATAAAAAGTTTGCTATTAATGTAATCTCGCAAACTGGCGAATGCTTAGAAACCTTAATAATGTTTAGAGAATTAAAAAACTTATTATATAAAGAGTTAGGCAAAGCAGGCGCTTCTAAATATATCTATGTAACGACAAACAATAATTATGGAAGATTATTTAATGATGCCAAGATAAATCAATATACTCATTTAACAATTTTAGATAATACAGTCGAAAGATTCTTAAGCTATTCACCTGCAATTTTATTGCCAATTGCTTGTATAGGAATTAATATTGAAAAATATTTATCTGGTGCTCATAAGGCTAATGAATTTTTTTTAAATGCACCATTAGAAAAAAACTCAGCAGTTTTATATGCAATTACTAGAAAAATTCTTCATGATAATAATTTTATATTTGAAAATGTTTGCTATTTCGAGCAAAATCAAGAATCCTTAGCAAAATTGTGA", "aa": "MENKQIIDINLKKAIKESAPNRYYETVQKILLDIKNSKVKNSNYLGVFNVFKNIKKSDYEKIQEISSELLSDGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLVYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNSAVLYAITRKILHDNNFIFENVCYFEQNQESLAKL", "aa_hexdigest": "f504ec4a80c0e3ff4a93a3ac562b8780", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pgi" ], "truncated": "5-prime", "pseudogene": { "start": 484627, "stop": 485766, "observations": { "mutation": [ 484932 ], "stop": [ 484932 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E7", "query_cov": 1.0, "subject_cov": 0.6303854875283447, "identity": 0.9890000000000001, "score": 532.0, "evalue": 2.64e-188, "gene_start": 1, "gene_end": 278, "reference_start": 1, "reference_end": 278, "reference_sequence": "MENKQIIDINLKKAIKESAPSRYYETVQKILLDIKNSKVKNSNYLGVFNVFKNVKKSDYEKIQEISSELLSNGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLVYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNSAVLYAITRKILHDNNFIFENVCYFEQNQESLAKLWQMLFSETISSMNNRIYIETCLGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLGWIMSFLHHSSILFAYLNEQNPFERKPLESYNFNLAKNFKELLGGKKKWLK" }, "paralog": false, "identity": 0.9894459102902374, "score": 745.0, "evalue": 9.8e-275, "description": "Internal stop codon at 484932. Nonsense mutation around 484932.", "psc": { "uniref90_id": "UniRef90_D1J8E7", "gene": "Pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "uniref50_id": "UniRef50_D1J8E7", "cog_id": "COG0166", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E7", "UniRef:UniRef50_D1J8E7", "COG:COG0166", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8E7" ], "product": "Glucose-6-phosphate isomerase (GPI)" } }, "id": "LMJBGEANGM_848", "locus": "GCA013368015_00839" }, { "type": "cds", "sequence": "contig_1", "start": 485756, "stop": 485995, "strand": "-", "frame": 2, "gene": "yabR", "product": "30S ribosomal protein S1", "db_xrefs": [ "COG:COG1098", "COG:R", "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6", "UniRef:UniRef90_D1J8E8" ], "nt": "TTGAGTATAAATGAAGTTAGTGATTATTACATTAGCGATTTAAAATTAATGTTTAAAATCAATGATATTAAGGAAGTTCAAGTAATTGAAATAATGCCAAATAATGATTTAGTAGTTTCATTTAAAAGAATTCACCCCAAGGAATTAAGAAACCCTTTTGAATTTAAACTAGATAGTCAAGATGCAGAATTTGATGGATTATTAGAATTTACTAACAAAGGAATTAATTATGGAAAATAA", "aa": "MSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK", "aa_hexdigest": "e2ea816ef770b351b97bd2726dfdb568", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "yabR" ], "truncated": "3-prime", "pseudogene": { "start": 485756, "stop": 486091, "observations": { "mutation": [ 486043 ], "stop": [ 486043 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E8", "query_cov": 1.0, "subject_cov": 0.7117117117117117, "identity": 0.987, "score": 159.0, "evalue": 2.4e-49, "gene_start": 1, "gene_end": 79, "reference_start": 33, "reference_end": 111, "reference_sequence": "MNYEIGDIFKAKIIKVWKNNIILQTKEGCKCFLSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK" }, "paralog": false, "identity": 0.990990990990991, "score": 222.0, "evalue": 1.42e-76, "description": "Internal stop codon at 486043. Nonsense mutation around 486043.", "psc": { "uniref90_id": "UniRef90_D1J8E8", "gene": "YabR", "product": "30S ribosomal protein S1", "uniref50_id": "UniRef50_A0A3T0TTP6", "cog_id": "COG1098", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E8", "UniRef:UniRef50_A0A3T0TTP6", "COG:COG1098", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3T0TTP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6" ], "product": "S1 motif domain-containing protein" } }, "id": "LMJBGEANGM_849", "locus": "GCA013368015_00840" }, { "type": "cds", "sequence": "contig_1", "start": 486290, "stop": 486433, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAATAGTTTTTATAAATGCTTAAACATTGCAACATTGACAGTTTCCTTGCTTTCAACTAGTCCTATTTTGATGATTAGCTGTGATGTAAAGAAAGATGTTGATTTATTGCATTTTAAAATGACTGTTAATAAACCTTGA", "aa": "MKNSFYKCLNIATLTVSLLSTSPILMISCDVKKDVDLLHFKMTVNKP", "aa_hexdigest": "2344ee639bdcf661fb955d6e442fd3ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5302.363400000001, "isoelectric_point": 9.139634513854983 }, "id": "LMJBGEANGM_850", "locus": "GCA013368015_00841" }, { "type": "cds", "sequence": "contig_1", "start": 486578, "stop": 486775, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCATTTATAACATCAACTTTATTAAAAAATTCATTGCTAAAAAATGATGTTATTCCTTTACTCCAAACAAAAACAAGGGCTTTTTATTTCGACAAATTAGATGAACAATATGTTGATGGTTCTTGCAATGATCCACTAAGAAAAATAGCAAAAATAGCAAATGATTTATTTAACAAAAAGCCTTATAAATCATGA", "aa": "MAFITSTLLKNSLLKNDVIPLLQTKTRAFYFDKLDEQYVDGSCNDPLRKIAKIANDLFNKKPYKS", "aa_hexdigest": "684cb420030135751e4de45e28c1f137", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7497.645000000002, "isoelectric_point": 9.37417049407959 }, "id": "LMJBGEANGM_851", "locus": "GCA013368015_00842" }, { "type": "cds", "sequence": "contig_1", "start": 487445, "stop": 487678, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGAAATTTTATCAACGCATGATGAAGATAAAACTGATGCGCCAACCATTGTAGGCAATATCGTTGCCCCTATAAGTAAATATTTTTTAAATTTTAGCATAAAAAATCCTCCTAAAAAGAGGATGCAAAAAACTAAGACACTCACTACGCTAGTATTAACTAGATCAGCTAATGAGGGTATTTCTCAGCCAAAGGCACCCCTGTTCTTTATAAAAAAATTATATACTTAA", "aa": "MFEILSTHDEDKTDAPTIVGNIVAPISKYFLNFSIKNPPKKRMQKTKTLTTLVLTRSANEGISQPKAPLFFIKKLYT", "aa_hexdigest": "f92d45febfd90232c63ae18a8a3d7a79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8727.155799999993, "isoelectric_point": 9.85201015472412 }, "id": "LMJBGEANGM_852", "locus": "GCA013368015_00843" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 487463, "stop": 487550, "strand": "+", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 57.5, "evalue": 4e-07, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGC", "id": "LMJBGEANGM_42" }, { "type": "cds", "sequence": "contig_1", "start": 487896, "stop": 488075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACAATCTAGAAAAAGATAAATCAAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGATTAGATGATGATCCGCTAAGAAAAATAGCTCAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGA", "aa": "MYNLEKDKSNIHPFLQTMTRAFVFDQDFNAQYSNGLDDDPLRKIAQSAFDLFNKKPFEN", "aa_hexdigest": "29056b1ccd79ce8e4a91692201dfe7df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6975.697100000001, "isoelectric_point": 5.030216789245605 }, "id": "LMJBGEANGM_853", "locus": "GCA013368015_00844" }, { "type": "cds", "sequence": "contig_1", "start": 488253, "stop": 488627, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACGGAAGTAGTTCTAGTGGTTCAAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAATGAAACAAAATATATTAAAGGCAAGATTAAAAATATTGGAAAGGGTTCATTTTCTAAATACCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACTCACAGCAAAGGTAGCACAATCTATAAATTAGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACCGAACCTTTAAAATATAACATTTTTGCTACATCATCAAATATTCCAAAAGAATTAAGAGAAATAATTGCAAATGCAATGGTTGAAGTTTGA", "aa": "MHGSSSSGSKYLLQEALFKKHFNLKGNEFKSFLEDKQGNETKYIKGKIKNIGKGSFSKYHIVFDDLGSFAYTHSKGSTIYKLDDKNSKIEFLTVTEPLKYNIFATSSNIPKELREIIANAMVEV", "aa_hexdigest": "2de6ded7ac035fbef3f688c73e7a0d31", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14050.860399999981, "isoelectric_point": 9.30170803070068 }, "id": "LMJBGEANGM_854", "locus": "GCA013368015_00845" }, { "type": "cds", "sequence": "contig_1", "start": 489464, "stop": 489775, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTATCCAAATTCAATCAATGTCAATTACTTTCATGAGCTTCTAATTCCACTGCTAATATTAGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTGTTGTTTTATTCAAGGACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAGCAATTGCAATGGGATTAATAATAACTATCTTTGCATGTTTATCATTAAGAGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAAGAGTTATTTAATGGTGAATGA", "aa": "MIYPNSINVNYFHELLIPLLILATFLIFIEFCNFIISNLLFYSRTQKVNIKKYAKNSTIKKIIIFIAIAMGLIITIFACLSLRGEKFYSTQGNYYFKELFNGE", "aa_hexdigest": "990bd27a7217a299a82637491e7ff77f", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12050.256599999986, "isoelectric_point": 9.329364967346194 }, "id": "LMJBGEANGM_855", "locus": "GCA013368015_00846" }, { "type": "cds", "sequence": "contig_1", "start": 490007, "stop": 490333, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTAGAGAGCTCCATTAAAAAAATCTCGACAAACAAGATAAATGAACTAAAAATAAAAGGATATTCAAAATTTTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATACGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCTGATATTGCGCCATATCTATGA", "aa": "MFNKLEAAFVIAFAIHSSSTISRMLESSIKKISTNKINELKIKGYSKFYIFRNFIMPTIKLDLITFMTFEIEKITRNFITYGLFSASLLGSETNLGRYKEISDIAPYL", "aa_hexdigest": "47327d05e1ae3056344029c963150f8f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12436.487199999983, "isoelectric_point": 9.55900135040283 }, "id": "LMJBGEANGM_856", "locus": "GCA013368015_00847" }, { "type": "tRNA", "sequence": "contig_1", "start": 490579, "stop": 490652, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 64.3, "nt": "AGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 490611, 490613 ], "id": "LMJBGEANGM_22", "locus": "GCA013368015_00848" }, { "type": "cds", "sequence": "contig_1", "start": 490797, "stop": 491417, "strand": "-", "frame": 1, "gene": null, "product": "Methionine adenosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377WSC8" ], "nt": "ATGAAAAGCCAAGTAACATTGGAATATGATGATGAAAGCAATAAAATAACTGTTAAAAAAGTTTTAATGTCAATTCAACATACTGAAAATGCAAATTTAAAAGAATTTAAAGATTTTATAAAGAAAAATATTGTTGATTATGTGTTAAAAGAATTTAATTTCAATTTGGATTATGATTTATTGATTAATCCAACTGGTAGATTTGTAATTGGAGGTCCTGTTGGAGATACTGGGCTTACAGGAAGAAAAATAATTGTTGATACATATGGTGGTTTTGCTCACCATGGTGGAGGAGCATTTTCGGGAAAAGATGCTACAAAAATAGATAGGTCCGCTGCTTATATGGCAAGGTATATTGCTAAAAACTTAGTTGCTGCTAAGATAGCAGACAAGATTGAAATTCAATTGTCTTATGCAATAGGCTTGCCACGTCCTCAATCTATTTATATAAATTCATTTAATTCATCACAATATAATGATGAACAAATTGCTGACATTATTGAACATGTATTTGATTTAAGCGTTGAACAAATTATAAAAAAATTAGATTTATTAAAGCCAATATATCTACAAACTGCAACATATGGACATTTTGGACGAAACGACCTTAATTTAAGTTGA", "aa": "MKSQVTLEYDDESNKITVKKVLMSIQHTENANLKEFKDFIKKNIVDYVLKEFNFNLDYDLLINPTGRFVIGGPVGDTGLTGRKIIVDTYGGFAHHGGGAFSGKDATKIDRSAAYMARYIAKNLVAAKIADKIEIQLSYAIGLPRPQSIYINSFNSSQYNDEQIADIIEHVFDLSVEQIIKKLDLLKPIYLQTATYGHFGRNDLNLS", "aa_hexdigest": "6def66330002956e54f20230115ba118", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010E99B0D", "query_cov": 0.9902912621359223, "subject_cov": 0.9577464788732394, "identity": 0.5539999999999999, "score": 233.0, "evalue": 5.98e-75, "valid": false, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_A0A377WSC8", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_A0A377WSC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377WSC8" ], "product": "Methionine adenosyltransferase" }, "genes": [], "id": "LMJBGEANGM_857", "locus": "GCA013368015_00849" }, { "type": "cds", "sequence": "contig_1", "start": 491679, "stop": 491885, "strand": "-", "frame": 1, "gene": null, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "nt": "ATGAAAAAATTATTAACATCAGAAAGTGTAGGGGCAGGTCACCCTGATAAAATATGTGACCAAATTAGTGACGCCATATTAGATAGTTTGTTGACAAATGATCCATATGCAAGAGTAGCTTGTGATGTTTTAGCAAACAACAACATCATTTATATAGGCGGTCAAATAACTACAAAGGCATATTCTGACACAATTAAAGAAGCATGA", "aa": "MKKLLTSESVGAGHPDKICDQISDAILDSLLTNDPYARVACDVLANNNIIYIGGQITTKAYSDTIKEA", "aa_hexdigest": "8e03e4d2757fc48613e17f520dcae434", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387A9BEE", "query_cov": 1.0, "subject_cov": 0.9444444444444444, "identity": 0.5589999999999999, "score": 81.3, "evalue": 6.23e-19, "valid": false, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_UPI00387A9BEE", "kegg_orthology_id": "K00789" }, "pscc": { "uniref50_id": "UniRef50_UPI00387A9BEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_858", "locus": "GCA013368015_00850" }, { "type": "cds", "sequence": "contig_1", "start": 492129, "stop": 492392, "strand": "-", "frame": 1, "gene": null, "product": "Aspartate--ammonia ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "nt": "ATGTTGAATGGGGATTTAATTGTTTATGATTCAGTAAATAAAAAAGCATTGGAAATTAGCAGTATGGGAATTAGAGTTGATAAAGATTCTTTAATTAAACAATCAAATAAAACCGAAGAAGAAATTAAAAAATTAAGTCCATATCATTGTGATTTAATAGAAGGCAAATTGCCATATACGATTGGTGGTGGCCTAGGCCAAAGTAGAATTGCAATGTTTCTATTAGAAAAACATCATATTGGTGAAGTTCAAGCATCTGCATGA", "aa": "MLNGDLIVYDSVNKKALEISSMGIRVDKDSLIKQSNKTEEEIKKLSPYHCDLIEGKLPYTIGGGLGQSRIAMFLLEKHHIGEVQASA", "aa_hexdigest": "9c327a574bde271a5fce32e86d2e8ad5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZXQ9", "query_cov": 0.9655172413793104, "subject_cov": 0.8, "identity": 0.655, "score": 117.0, "evalue": 1.62e-32, "valid": false, "product": "Aspartate--ammonia ligase", "uniref50_id": "UniRef50_A0A448ZXQ9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "product": "Aspartate--ammonia ligase" }, "genes": [], "id": "LMJBGEANGM_859", "locus": "GCA013368015_00851" }, { "type": "cds", "sequence": "contig_1", "start": 492393, "stop": 492587, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAGTCAAAAGTTAGCTAAAAATATTTATTTTATTAGTTCTAGCCATTTGTATAATCTTTATCCAAACCTAAGTCCCGAAGAACGTGAAGAAGCAATTTGCAAAGAATATGGTTCTGTTTTTATTTATCAAATAGGATACAATTTGCCTGATGGAATTCCACATTCAAAAAGAGCATTCGACTATGATGATTGA", "aa": "MSQKLAKNIYFISSSHLYNLYPNLSPEEREEAICKEYGSVFIYQIGYNLPDGIPHSKRAFDYDD", "aa_hexdigest": "1c921a58ad1b4b8aaf168dea11ff5f2d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 63, "aa_cov": 0.984375, "hmm_cov": 1.0, "evalue": 3.644573018208482e-23, "score": 78.02096557617188, "start": 2, "stop": 64 } ], "seq_stats": { "molecular_weight": 7480.2697, "isoelectric_point": 5.167596626281735 }, "id": "LMJBGEANGM_860", "locus": "GCA013368015_00852" }, { "type": "cds", "sequence": "contig_1", "start": 492708, "stop": 492815, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAAAAAGTATGAATTTGGATTGTATGAAGGAATATATACAGATATGAATGCTATAAGAAAACAAGAAAAATTAGATTATAAACATTCAATATATGTAGACCAATGA", "aa": "MKKYEFGLYEGIYTDMNAIRKQEKLDYKHSIYVDQ", "aa_hexdigest": "e818bd17f6796d65e95127a34190ca8f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 35, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.001249618471376e-14, "score": 49.429931640625, "start": 1, "stop": 35 } ], "seq_stats": { "molecular_weight": 4318.879500000002, "isoelectric_point": 6.529004096984862 }, "id": "LMJBGEANGM_861", "locus": "GCA013368015_00853" }, { "type": "cds", "sequence": "contig_1", "start": 492828, "stop": 493058, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGCCATCATCAAAATTATCATTGAAAGATACCCAATTTGCTATTGAAAAATTAAAAGATTTATTTTCACATCAACTTCATAGAACATTGAATTTAGTCAGAGTTTCAGCGCCATTGTTTGTAAAAACTAAAAGTGGTTTAAATGACGGTTTGAACGGAGAAAAACCAGTTGTATTTTTTGCAGACGAAGTTGAAGATGAATTACAAATTATTCATTCACTTGCTAAATGA", "aa": "MPSSKLSLKDTQFAIEKLKDLFSHQLHRTLNLVRVSAPLFVKTKSGLNDGLNGEKPVVFFADEVEDELQIIHSLAK", "aa_hexdigest": "4ebbf96f33764ac1b55f29343995a790", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 8.570631847919875e-20, "score": 66.99182891845703, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8533.764299999999, "isoelectric_point": 6.824282646179199 }, "id": "LMJBGEANGM_862", "locus": "GCA013368015_00854" }, { "type": "cds", "sequence": "contig_1", "start": 493192, "stop": 493647, "strand": "-", "frame": 3, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGGATGCTGTAAAATTGGCTAACTTGTTGCAATACCAAGGTGCATTAAACGAAGCTCAATTGCGTAACATGCGTTTAGCATTAGACACTGCTTACCATGGTAAAGGTGTAAAAGGACATGAAGACTTGCCAGCAGGTGCTTCAATCAACCAAGTAAGAGAATTCATGAAGAAGAATTCATCACTAGGAATTGGTGACATAACATCAGAATCAAGACGTTACCTATCATATGTTGGTCAAGCAACCTCATATAACTCAGGTAAAGCAGTTATGATGGATCTATATACAAAAGTTCAAAAGAAACTAGGATTAACAAGAAGAGAATTTGTTGAAAAAGATAATCATAAATATGTAAAAGAATTCTTTGATGCTTTATTACGTAATAGTGCATTGCCAATGGATGCTTTAATTAAATCAGTAAGTGCTAAATATGGCCTAACTGTTGAAAAGAAATAG", "aa": "MDAVKLANLLQYQGALNEAQLRNMRLALDTAYHGKGVKGHEDLPAGASINQVREFMKKNSSLGIGDITSESRRYLSYVGQATSYNSGKAVMMDLYTKVQKKLGLTRREFVEKDNHKYVKEFFDALLRNSALPMDALIKSVSAKYGLTVEKK", "aa_hexdigest": "5e7d6e2594f9d508132513afbad2818f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.8675496688741722, "subject_cov": 1.0234375, "identity": 0.55, "score": 139.0, "evalue": 5.74e-40, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "LMJBGEANGM_863", "locus": "GCA013368015_00855" }, { "type": "cds", "sequence": "contig_1", "start": 493699, "stop": 493818, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGTTGACTTTTCAACAGCACATGGTATAACAAACTTCTTTACTGCTAAAACAGAAGCAGAAGTTACAGATGAAATGATTAAACAAATTAAAGACTTACACAATGGTGTATATTGA", "aa": "MPVDFSTAHGITNFFTAKTEAEVTDEMIKQIKDLHNGVY", "aa_hexdigest": "3b33a6e347fdc2ee67454780e561f76f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4399.907700000001, "isoelectric_point": 4.915288352966308 }, "id": "LMJBGEANGM_864", "locus": "GCA013368015_00856" }, { "type": "cds", "sequence": "contig_1", "start": 493903, "stop": 494019, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGACACCACAACCAATTAATGTATGCTCAAAAGTATCTATCATCAGTTGGAGAATTTGGTAAATATAAATTAGGAAATGTATTCCACTACACTTCATACGTTGAAGGTTGA", "aa": "MMGHHNQLMYAQKYLSSVGEFGKYKLGNVFHYTSYVEG", "aa_hexdigest": "064ced3e7916d6f765f037258ca96559", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4415.979300000001, "isoelectric_point": 8.093570518493653 }, "id": "LMJBGEANGM_865", "locus": "GCA013368015_00857" }, { "type": "cds", "sequence": "contig_1", "start": 494050, "stop": 494250, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGCATTTAAAGCATATGATCAATTTAAGAAGACTACTCAAAATTATGGACGTAAATTCTTTGCAAATGAAGTTCCTGATTATGATATTCAAACATATGACTTTTCTAGAAGAGAATTTGTAGGTGTTGGAGCTTACAACTCGGGCATTAAAAAATTTATGTTTAATGTTGACCCATACTTCTCATTACCAAAATGA", "aa": "MSAFKAYDQFKKTTQNYGRKFFANEVPDYDIQTYDFSRREFVGVGAYNSGIKKFMFNVDPYFSLPK", "aa_hexdigest": "34732396c0d0be84b91711ee8971d58a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7815.717, "isoelectric_point": 9.259674644470213 }, "id": "LMJBGEANGM_866", "locus": "GCA013368015_00858" }, { "type": "cds", "sequence": "contig_1", "start": 494477, "stop": 494653, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTTTTCGTCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCGTATTTAATTTTAGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAA", "aa": "MFPFSSRITTVSSFISSGPIPSASYLILASHFLPFPTIKSAAWLAIFFEFSADFFVVS", "aa_hexdigest": "ea56eb80e1eb0db2d5e379dfa934a12c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.334099999998, "isoelectric_point": 6.505302238464354 }, "id": "LMJBGEANGM_867", "locus": "GCA013368015_00859" }, { "type": "cds", "sequence": "contig_1", "start": 494566, "stop": 495117, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGAAAGTTTTCTCAAAAATGGTAATCAATGCCTTTGTGGGAAGTTTATTTCAAACACAACTAACTAATTTTGTAAATGATAAGTCTAAAAACGAAATTTCAGTTGTAGAATTAATTAATTCTTCTACCTTGCCAACAGCCAAAAAAGAATTATTAAAATATTATGCAACTACATATTACAATGCTGCAACTCATGGATTGGGTGAAGATATTAAAGAATTAAAACTTTATAAAGAAAATAAAGTTAATGAAAAAGAATTATCTATTGATGCAAAAAATGCTAGTGGGGAAATTGTTAAATTATATGGTTTAGGATTAACTGAAAAAGACTTAAATCAAAGAAATGTTGGTTTGGGTTTTGCTGAAGGAGATGCTACCGTAAATGGTCAATCGATGTATCGTCAAATTCTAAAGATGGCTACAACTTCTGATTTAACAGATGATCAAGTAAATAATATTGGTTATGAAACAACAAAGAAATCTGCTGAAAACTCAAAGAAAATTGCTAACCAAGCAGCTGATTTAATTGTTGGAAAAGGCAAAAAATGA", "aa": "MSKVFSKMVINAFVGSLFQTQLTNFVNDKSKNEISVVELINSSTLPTAKKELLKYYATTYYNAATHGLGEDIKELKLYKENKVNEKELSIDAKNASGEIVKLYGLGLTEKDLNQRNVGLGFAEGDATVNGQSMYRQILKMATTSDLTDDQVNNIGYETTKKSAENSKKIANQAADLIVGKGKK", "aa_hexdigest": "76260c2f9ed00d98cdb408235766e8b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20157.659099999993, "isoelectric_point": 8.842048454284669 }, "id": "LMJBGEANGM_868", "locus": "GCA013368015_00860" }, { "type": "cds", "sequence": "contig_1", "start": 495319, "stop": 496431, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAACTAGAATTCTAATTTCATTAGGTTCAATTTTTGGTGTAGCAGCCTTAATGCCTATGGCAGCATCATGTACACATACAAATAATGATGAATTAAACAAAAAAATTGAATCTTTAGAAAAGCAATTAAATGAAGCAAAAGCAAATTCTCAAAAAGATCAAGCACAAATTAATGATCTTATGAAGCAATTGCAAGAAACAAAAAATAATTCAAAAACAATTGAAGAAAAAGAAAAATTAGTTAAAAAATTGCAAGAAGATTTAAATAAAATCCAACAAGAAAGAACAGCTAAATATCAAGCAGAATTAAATTCTGCAAACAAGGCTATTGTTGAAATTCCTAATAAATATAAGGAACTAGAACAAAAAAATGATGATCTTGAATCCCAATTAAGTGGATTAGAAACTAAGCTAATGTCTTCTAAAAGCAAGCTTAAAAATTCAGGAAAAAAAATAGAAAATTATTTGAAAAAATCAACAGAAATAGAAAATAACATTAAAGAATCTAAAACAAAATTAGTTGAATTAAGAAAAGCTTTGGAAGAATTAAACAAGAATTTAGAAGCATTAGAAAAACAAAATTCTGATGGTTCAAAAAATGATGAAATATCATCAAAAAAAGAAGAAATTAAAGCTAAGAATTTAGAAATTAAGCAAGAAGAAGAAAAGTTTGAAGGTCTTCAAACAGAATTAAAAACTCAAAAAGATAAAATCATTGAAGAAACTAATACAAAGTCAGAACTTGAAATTAAAGTTGCAGATTTAAAAGCAAAAAAAGAAAATATTTCTAAAGAAATTGAAGCTAATAAAAAGGAATTAGATGCGCTAGAAAAGGAATATAATGCTAGCGTTAAAAAAAGCGATGAATTAAGCAATTTACTTTCAAAAGAAAATGATAATTCGCCAGCTAGTCAAGAAGCAGCCGCAAGACACATCTTGGATTTAAAAGATGATCTACAATCTGAATTAAAGAAAGAAAAATTAAATGGAATAACCGCTCCTACTGCCGAACAATCAAAGAAAATTTCTGAAATTTATGGTAAATATATTGAAAAAATTTCAAAAATAAATGACGCTTCACTAACAAGCGATTCTTTAGCATGA", "aa": "MKKQTRILISLGSIFGVAALMPMAASCTHTNNDELNKKIESLEKQLNEAKANSQKDQAQINDLMKQLQETKNNSKTIEEKEKLVKKLQEDLNKIQQERTAKYQAELNSANKAIVEIPNKYKELEQKNDDLESQLSGLETKLMSSKSKLKNSGKKIENYLKKSTEIENNIKESKTKLVELRKALEELNKNLEALEKQNSDGSKNDEISSKKEEIKAKNLEIKQEEEKFEGLQTELKTQKDKIIEETNTKSELEIKVADLKAKKENISKEIEANKKELDALEKEYNASVKKSDELSNLLSKENDNSPASQEAAARHILDLKDDLQSELKKEKLNGITAPTAEQSKKISEIYGKYIEKISKINDASLTSDSLA", "aa_hexdigest": "d19c9f99777731c4bc9c46b7cfed98f4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41910.93720000005, "isoelectric_point": 6.395887184143067 }, "id": "LMJBGEANGM_869", "locus": "GCA013368015_00861" }, { "type": "rRNA", "sequence": "contig_1", "start": 496793, "stop": 496897, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.3e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "LMJBGEANGM_37", "locus": "GCA013368015_00862" }, { "type": "cds", "sequence": "contig_1", "start": 497107, "stop": 497259, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCCTCATTTCTATTATTATTTCTATGTATATAAATATGCATTAGGATATATCGTAGCAAATGTATTTTTCCAAAAATACAAACAAGAAGGCCAAGAAGCATTAAAAAACTATATAAATAAATTTTTAAGTGCCGGAGATATCGATTGA", "aa": "MVPHFYYYFYVYKYALGYIVANVFFQKYKQEGQEALKNYINKFLSAGDID", "aa_hexdigest": "ccab9e932d67397b07838c4ee1e3418c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.8657, "isoelectric_point": 7.928853797912598 }, "id": "LMJBGEANGM_870", "locus": "GCA013368015_00863" }, { "type": "cds", "sequence": "contig_1", "start": 497416, "stop": 497700, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTTATATATTGATGAATTTTGATGGAACATTGGAATCAGTCAATACATTATGCCACGAAATGGGACATTCAATGCATTCATATTTTTCTAATAAATGCCAATCTCCACTTAGAAGCTCTTATCCAATATTTTTAGCAGAAATTGCTTCGATATTTAATGAATTATTACTAAATGATTATTTAATAAGCAAATCAAAAAATATTCAACAACAATTTTATCTACTTGATTCAAGCATTAATGATTTTATTGGCACAGTTTTGCGTCAAACGATGTGA", "aa": "MKKIYILMNFDGTLESVNTLCHEMGHSMHSYFSNKCQSPLRSSYPIFLAEIASIFNELLLNDYLISKSKNIQQQFYLLDSSINDFIGTVLRQTM", "aa_hexdigest": "6a78944ec2b0ad72b925be9724fc6b90", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10891.465399999988, "isoelectric_point": 6.241057777404785 }, "id": "LMJBGEANGM_871", "locus": "GCA013368015_00864" }, { "type": "cds", "sequence": "contig_1", "start": 497899, "stop": 498381, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAACTTGATCATAAATTGTCGGATGATGTTGAAAATTATTTAAATGATACTGCAAGTGGTTCGCCTGTACCTGAAGATATTTTTTCAATAATAAGCGATAGTGAAGTTGATTTTGGCAATGTTTTAATTAAAAATAAAAAAATAAAAATAACCGAAGGAAATAGAATAGCTTTATTAAAAAATAAAGATGAACAAGTTAGAAAAGATGTTTATTTTAATTATTTAAATGGATATTTAAAAAACAAACAATCATTAGCGAGATTGCTATATCAACATATTAAAGAAATATCAGTTAATGCTTTATATAGAAAATATAATTCATCTCTTGAATCTATTTTGTTGCAAGATCATGTTGATAAAAAGTTATTGAACATTATTTATGATGCAGTTCAAAAACATATGTATATATTTAAAAAATATAACAATGCTAAAAAACAATTTTTTAAAGCTAAATTCAATAAAAAATTAGAAAAATGA", "aa": "MQELDHKLSDDVENYLNDTASGSPVPEDIFSIISDSEVDFGNVLIKNKKIKITEGNRIALLKNKDEQVRKDVYFNYLNGYLKNKQSLARLLYQHIKEISVNALYRKYNSSLESILLQDHVDKKLLNIIYDAVQKHMYIFKKYNNAKKQFFKAKFNKKLEK", "aa_hexdigest": "8cd7040db18df7e979293f3522fcdff7", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18823.412900000003, "isoelectric_point": 9.33084774017334 }, "id": "LMJBGEANGM_872", "locus": "GCA013368015_00865" }, { "type": "cds", "sequence": "contig_1", "start": 498430, "stop": 498693, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAAAAATAATTGAAATAAAAGATAGCAAATACAATTCATTAGGCCAATATGTTGAATATATTGAGCTTTCAAAGAAATTCTTAATTTTAGGCAATAGAATTTCTAATTATTTAGATAATAAATTAAATATAAATATTGTTGATCCCACAATAAATAAAACAATTTCATTATTTGAAACAAAATCCCAAGAATATGCTAAAAAGTTAGGTTCAGAAGCCAATAGAATTTTTCAACATAAAGACAAAATCAAAACATGA", "aa": "MYSKIIEIKDSKYNSLGQYVEYIELSKKFLILGNRISNYLDNKLNINIVDPTINKTISLFETKSQEYAKKLGSEANRIFQHKDKIKT", "aa_hexdigest": "ce6d2dee0de2a258441730ef1b8dbc12", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10190.63739999999, "isoelectric_point": 9.42580966949463 }, "id": "LMJBGEANGM_873", "locus": "GCA013368015_00866" }, { "type": "cds", "sequence": "contig_1", "start": 498804, "stop": 499319, "strand": "-", "frame": 1, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0003999", "GO:0009116", "KEGG:K00759", "RefSeq:WP_012855642.1", "SO:0001217", "UniParc:UPI0001BC2388", "UniRef:UniRef100_D1J8F6", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_D1J8F6" ], "nt": "ATGGATTTAAAAAAGTATATAAGAACTGTTGATGACTTTCCACAAAAAGGCGTCAAATTTAAAGATATTTCGCTTTTATTAGCCAATGGACAAGCACTACATTATACAATTGATGAAATGGCTAAATTAGCAGAAGGTGCAGATATTATTGTGGGTCCTGATGCAAGAGGATTTTTATTCGGAACACCAGTAGCTGCTAAATTATCAAAACCATTTATTATGGTAAGAAAAAAAGGCAAATTGCCCGGCGAAGTTATAAGCTGCGATTATGGACTTGAATATGGTAGAAATATTTTAGAAATTCAAAAAGGTTTAGTTAAACCAGGACAAAAAGCCGTCATAGTTGACGATGTTTTGGCAACTGGGGGTACATTGGAAGCAATTACTAAATTATTAACTGAACAAGGTGTAATTATCAGTAGAATAATTGTTTTAATGGAATTAGATGGCTTTAATTCTCATGAAAGATTGCCATACGATATAAAAAGTTTAATCTATATTAATAAAGGGGAATAA", "aa": "MDLKKYIRTVDDFPQKGVKFKDISLLLANGQALHYTIDEMAKLAEGADIIVGPDARGFLFGTPVAAKLSKPFIMVRKKGKLPGEVISCDYGLEYGRNILEIQKGLVKPGQKAVIVDDVLATGGTLEAITKLLTEQGVIISRIIVLMELDGFNSHERLPYDIKSLIYINKGE", "aa_hexdigest": "e1df95e8030d659ee05d448dda0526e2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2388", "ncbi_nrp_id": "WP_012855642.1", "uniref100_id": "UniRef100_D1J8F6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2388", "RefSeq:WP_012855642.1", "UniRef:UniRef100_D1J8F6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8F6", "uniref90_id": "UniRef90_D1J8F6" }, "psc": { "uniref90_id": "UniRef90_D1J8F6", "gene": "Apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0003999", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "LMJBGEANGM_874", "locus": "GCA013368015_00867" }, { "type": "cds", "sequence": "contig_1", "start": 499533, "stop": 499772, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTAGATGAAAACGGCAAAAATTGTTATGTTTTAGAAATAAATAACAAACTATTTGTGATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGA", "aa": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIP", "aa_hexdigest": "9227c53fcb884f46403e9711cc4e8abd", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8967.135899999994, "isoelectric_point": 5.212613105773924 }, "id": "LMJBGEANGM_875", "locus": "GCA013368015_00868" }, { "type": "cds", "sequence": "contig_1", "start": 499779, "stop": 500153, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAATAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCGTTTGGCGGGGTAAATGTAACTCCTTTTGATCTAGCCGGAGGATTACCTGGACAACTTGGGTTCAATTTTGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAATAAAAAGGAATTACAGCTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGA", "aa": "MKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIQQIINNKKELQLLIMDSSRSAFPGRSIEKL", "aa_hexdigest": "c211546d96dcb2aa95dca5bf55cfb2a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13803.850099999985, "isoelectric_point": 9.559452629089353 }, "id": "LMJBGEANGM_876", "locus": "GCA013368015_00869" }, { "type": "cds", "sequence": "contig_1", "start": 500232, "stop": 501188, "strand": "+", "frame": 3, "gene": "rnjA", "product": "mRNA degradation ribonuclease J1/J2", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8", "UniRef:UniRef90_D1J8F7" ], "nt": "ATGATAACTGCACATGAAATTTTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAACCCAAATTCTCAATTTCCAGAATTCGTTGAATATCAAACTGCAAATAATGTTAAAAATGCCGTTATTCTTGTAACGGGCGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGACAATGATGTTTATTTAAAATTGAAAAATGATGATGCAGTCATAATAATTGCTCCACCAGTTAACGGATTGGAAGTAAATTACGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTTCTAAATTAGATATTTTTAATATTATTAAAGCATTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCTAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCTGAATTCAACGATACAAATTTAATTTCTCAAAAACATGGAATTAGAAATGTAGGTGATGTTATTATTGATGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACTTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTTGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAAAGAAAAATCGCCAAAACTATTGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAA", "aa": "MITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPSKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI", "aa_hexdigest": "032091a0061d2b258c17e6df2fe8b98b", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnjA" ], "truncated": "5-prime", "pseudogene": { "start": 499932, "stop": 501188, "observations": { "mutation": [ 500151 ], "stop": [ 500151 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8F7", "query_cov": 1.0, "subject_cov": 0.5771324863883848, "identity": 0.997, "score": 570.0, "evalue": 1.1e-200, "gene_start": 1, "gene_end": 318, "reference_start": 234, "reference_end": 551, "reference_sequence": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIPWLLMKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIKQIINNKKELQLLIMDSSRSAFPGRSIEKLWVSRKIEYKFKETSDNQRIIVGLYDEDMITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI" }, "paralog": false, "identity": 0.9904306220095693, "score": 764.0, "evalue": 1.36e-279, "description": "Internal stop codon at 500151. Nonsense mutation around 500151.", "psc": { "uniref90_id": "UniRef90_D1J8F7", "gene": "RnjA", "product": "mRNA degradation ribonuclease J1/J2", "uniref50_id": "UniRef50_A0AAJ5NJJ8", "cog_id": "COG0595", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8F7", "UniRef:UniRef50_A0AAJ5NJJ8", "COG:COG0595", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5NJJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8" ], "product": "Ribonuclease J" } }, "id": "LMJBGEANGM_877", "locus": "GCA013368015_00870" }, { "type": "cds", "sequence": "contig_1", "start": 501688, "stop": 502233, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E31DEDA", "UniRef:UniRef90_UPI001E31DEDA" ], "nt": "GTGATTGGAACATTTATTAATTCAATATTAAATATTCCTGGAAAAAAACTCGGTTTCTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGCTTATCTGTCATTTCATTTTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAGGAAGAGGATATTGAAATAATTGATAACAAAGAAGTCGTAAATAGCAATATTCAAATTTTAGAGGCTAATTCTAACTCAAATAAAAATATAGACTCAGCACAAAATTTAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGACGAAAAAGAAATTGAAGAAGATTCAAAGCAAAAAGACAACAATGAGCTAGAGCCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGGTCCTACACTACAAGAACCAGATAATTCAAATAAAAATAAAGAGGTTAAAAAAGAAGATAAGATCTATAAAATCATTGAAGATGAAGAAAATTTGTTCTAA", "aa": "MIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSAQNLNQNNNLDLDPFDDEKEIEEDSKQKDNNELEPKEAIDSPSNENSIGPTLQEPDNSNKNKEVKKEDKIYKIIEDEENLF", "aa_hexdigest": "4bbbbeb7fcec32cdbebbeb45ea11bf81", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 501550, "stop": 502233, "observations": { "mutation": [ 501628 ], "stop": [ 501628 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E31DEDA", "query_cov": 1.0, "subject_cov": 0.7973568281938326, "identity": 0.972, "score": 265.0, "evalue": 1.24e-87, "gene_start": 1, "gene_end": 181, "reference_start": 47, "reference_end": 227, "reference_sequence": "MGSVIYYTIHQVEHNSKNFFTKYFSSWFVDFSNSSNYLLPQKYTPGVIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSAQNSNQNNNLDLDPFDDEKEIEEDSKQKDNNELEPKEAIDSPSNENSIDPTPQKPDNSNENKEVKKEDKIYKIIEDEENLF" }, "paralog": false, "identity": 0.9691629955947136, "score": 384.0, "evalue": 3.42e-137, "description": "Internal stop codon at 501628. Nonsense mutation around 501628.", "psc": { "uniref90_id": "UniRef90_UPI001E31DEDA", "uniref50_id": "UniRef50_UPI001E31DEDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E31DEDA", "UniRef:UniRef50_UPI001E31DEDA" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E31DEDA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E31DEDA" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20625.802300000007, "isoelectric_point": 4.567547416687013 }, "id": "LMJBGEANGM_878", "locus": "GCA013368015_00871" }, { "type": "cds", "sequence": "contig_1", "start": 502597, "stop": 503547, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81", "UniRef:UniRef90_UPI00215D7B81" ], "nt": "ATGAAAAACAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCATTAACATTGATTGCATCTGCCATCACGATACCCGTTGTAATTTCATCCTTAATTATAAATAAGAATAATTGGGCTAACAAATACTATAGCCAAAGATCAGAGGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCTTAGAGGATCAAAATAATTTAAATAAGTTATTGAATAAAATTAATTTAACTAGCAAAAATAGTTCTGCTGATTTTAAAAACTCATATAATTTGCTTAACAAGACAAATCTTGATATCAACAAAAAGATAAATATTAACAAAAAAGAATTTGACAAAACTTATGCTGATATTTTGCTTTTAAGCCCACAGTTGCAAGATTTATTCAATAAAATTATTGATTTGAAATTGAACAACAATATAAAAGAAGATGTTGATGGACTATTACAAAGAGTTGAAAAAGATCTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGATATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTAAATTCATATTATAAATTAATTAACAGCAAAGATAAAACATATAAAGGCATTGTCTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATGGCAATGAAATAAACCATCTTAAAAAGAATTTTAAAAATTTCCAATCAGTTGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAGGAAATTAAAGAAGAAATTGTTGAACATAGAAAGCTGAGCCAAAAGATAAAAAAAGATGGAAATGATATTTTAAATAATGATTTTGATAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCGAAATCATTTTTATTAATGTGA", "aa": "MKNKKSKKIVVALSLTLIASAITIPVVISSLIINKNNWANKYYSQRSEASHLVNLIEKNKHFYYFALEDQNNLNKLLNKINLTSKNSSADFKNSYNLLNKTNLDINKKININKKEFDKTYADILLLSPQLQDLFNKIIDLKLNNNIKEDVDGLLQRVEKDLNKCRKIDENINLIDIKEIYAHSSKTIIDVSNQLNSYYKLINSKDKTYKGIVSEIKDLKKDYFNKIEDGEEYYKLYGNEINHLKKNFKNFQSVEDYNNYINYANSKIKEIKEEIVEHRKLSQKIKKDGNDILNNDFDKSALIRNSYFQEPKSFLLM", "aa_hexdigest": "05a96df10c6be84d0470d6bc539b4b4f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00215D7B81", "query_cov": 0.9683544303797469, "subject_cov": 0.8116710875331565, "identity": 0.993, "score": 528.0, "evalue": 4.1e-187, "valid": true, "uniref50_id": "UniRef50_UPI00215D7B81" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D7B81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37043.94010000002, "isoelectric_point": 9.269409370422363 }, "id": "LMJBGEANGM_879", "locus": "GCA013368015_00872" }, { "type": "cds", "sequence": "contig_1", "start": 503869, "stop": 504189, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA8CC", "UniRef:UniRef90_UPI00215DA8CC" ], "nt": "TTGTTTTATGACAAGGTTATTCAAGACCCAAAAACTAACGAAATTGATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCGGAACAAGATATTAGATATTTACACTACCATTTTGGAAGTCTCCTAACTCAAAAGGTTAATGAAATAATTAGGCATTATAATCAAAAAGCAAAAGCCTTATTAGAAAAAATAAAAAACAATAATAGTTACGAAACACAAAAAAATAGAATAGAAAAATTACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGCGATTTCAATGATTACGAAAAATTCTATTGGTGA", "aa": "MFYDKVIQDPKTNEIDEEKLMKLDDIHFDPEQDIRYLHYHFGSLLTQKVNEIIRHYNQKAKALLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYEKFYW", "aa_hexdigest": "57ca8f0490bd70a5c98b46f7450174e5", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 503869, "stop": 504333, "observations": { "mutation": [ 504187 ], "stop": [ 504187 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA8CC", "query_cov": 1.0, "subject_cov": 0.6883116883116883, "identity": 0.981, "score": 208.0, "evalue": 1.81e-67, "gene_start": 1, "gene_end": 106, "reference_start": 1, "reference_end": 106, "reference_sequence": "MFYDKVIQDPKTNEIDEEKLMKLDDIHFDPEQDIRYLHYHFGSLLTQKVNEIIRYYNKKAKALLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYEKFYWWTYKFQKDSNGDIITSSNSEIPDFYKNEIEVAQYLHELDEILDNLKNI" }, "paralog": false, "identity": 0.974025974025974, "score": 311.0, "evalue": 1.82e-110, "description": "Internal stop codon at 504187. Nonsense mutation around 504187.", "psc": { "uniref90_id": "UniRef90_UPI00215DA8CC", "uniref50_id": "UniRef50_UPI00215DA8CC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA8CC", "UniRef:UniRef50_UPI00215DA8CC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA8CC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA8CC" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13022.539399999981, "isoelectric_point": 6.414700889587402 }, "id": "LMJBGEANGM_880", "locus": "GCA013368015_00873" }, { "type": "cds", "sequence": "contig_1", "start": 504576, "stop": 504710, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGAGAAAGAAATTAATTGCTTTATCTGTGGCTTTTGGACTTTTAGCGCCTATTTCAGGAACTCTTATGATTTCATGCAAAAACGAAAAAAGTAATGCTGAATATCAAAAAGAACTGCAAAATAAATGA", "aa": "MKMRKKLIALSVAFGLLAPISGTLMISCKNEKSNAEYQKELQNK", "aa_hexdigest": "d6c4de5d36e2fb785b90fb5a80f9408b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4926.885100000001, "isoelectric_point": 9.807978248596193 }, "id": "LMJBGEANGM_881", "locus": "GCA013368015_00874" }, { "type": "cds", "sequence": "contig_1", "start": 504837, "stop": 505382, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04778.17" ], "nt": "TTGAAGAATTTTATTGCAAAAATTAATGAAACTACACAAGATATAAATGCACAAATTTCAAAATTAAAAAACGAAAACGGAAATACAAACCCTGAAAACAATAGCCAAAATGATTTATTAAAAAAATTAAAAACAGAATTGGAAACGGCAACTAAAAAACTTAACATGCTACTTTCGGCAAATGAAAGTAAAAACATCTCAACAAAAGAAGAAGCTAAAAACATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAACAAGAATATAAAAATCAAATTCAAAAAGTAAATGAAACTATCAATAAAATTGAAGAAGAAATATCAAATTTAGAAAAAGGCCCAAAATCAAAAGAATTTACTAAATTAAAAGAATTAAGAGAAAAGCTAACAAATTACTTAAATAGTAATTTCTCTGAATTTGCTTATAAAGATTTAAGAGCTGAAATTAAAGAATTAATTCAAAAACAAGAAAACGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTATGCAAATAAATGATATTTTTTGA", "aa": "MKNFIAKINETTQDINAQISKLKNENGNTNPENNSQNDLLKKLKTELETATKKLNMLLSANESKNISTKEEAKNILNAANKALEKSNEQEYKNQIQKVNETINKIEEEISNLEKGPKSKEFTKLKELREKLTNYLNSNFSEFAYKDLRAEIKELIQKQENVSIVSNEKEIKSAIMQINDIF", "aa_hexdigest": "08b23d479efd6e523abe317dc1846fc6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 129, "aa_cov": 0.7071823204419889, "hmm_cov": 0.9922480620155039, "evalue": 1.8374690904036284e-07, "score": 26.718767166137695, "start": 52, "stop": 179 } ], "seq_stats": { "molecular_weight": 20838.30550000001, "isoelectric_point": 7.737401008605957 }, "id": "LMJBGEANGM_882", "locus": "GCA013368015_00875" }, { "type": "cds", "sequence": "contig_1", "start": 505497, "stop": 506339, "strand": "+", "frame": 3, "gene": null, "product": "P75 protein precursor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RLP9", "UniRef:UniRef90_UPI000CD67922" ], "nt": "TTGAATACCTCGATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACTAAAATTTCTGATGTAGAAAAACAAAATTATATTGAAGAAGTTAAAAATACTATAAACTCTTTGTTGAACAAATATAATAATAATATAAATAACAATGAAGATAAAATTTCTGAATTAAATCGAAACATAGAACAATTAAAAAAATTAAATCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAATAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCCAAAAAAGCAATTTTACTAGAATCATTAAGAGATACCGTTTCGGAATTTCTTGAATTTTACTTACAATTAACCCATTATCTAAGAATCTTTTTTGATAAAAATAATGACATTATTAAATTATTACCAACAGATTCACAAACAAAAATTGATAATTATCTAGACGAAATTAAAAAAATTGCAGAAAAAAATAGTCAAACAAGAGGAAGCATACCAACATTTGACGAATATATTTCAGCCTTTAAGGCACAAGAAGAAATTTTTAAAATTATAAAATCTAGTAATCTAAAATCAGCAGATGTTAACATATTAAAAAAATTGACAGATAATTTAAAAGAAATTAAAAAACAAAAGAAAAATGATTATGGCACGATTGTTACTTCTTATTTAAAAGAAAAAATAGAAGAAACAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATGTTGAAAGTCAAAATATTATTGAAACTTTTGCTTTATTGCAAAAATTAGAACTTGAATTTGCTTATATTAATTGA", "aa": "MNTSIFENIETTNERNLLNKYIKTNLTKISDVEKQNYIEEVKNTINSLLNKYNNNINNNEDKISELNRNIEQLKKLNQIENNYVIDKVKLLNLLNKANTIKNEGSFAKKAILLESLRDTVSEFLEFYLQLTHYLRIFFDKNNDIIKLLPTDSQTKIDNYLDEIKKIAEKNSQTRGSIPTFDEYISAFKAQEEIFKIIKSSNLKSADVNILKKLTDNLKEIKKQKKNDYGTIVTSYLKEKIEETITKNESIIAKKSNVESQNIIETFALLQKLELEFAYIN", "aa_hexdigest": "1d07f67b42c20cc121cc0cef464a293d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 505197, "stop": 506540, "observations": { "mutation": [ 505380, 506337, 506439 ], "stop": [ 505380, 506337, 506439 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000CD67922", "query_cov": 1.0, "subject_cov": 0.43143297380585516, "identity": 0.8959999999999999, "score": 456.0, "evalue": 2.22e-155, "gene_start": 1, "gene_end": 280, "reference_start": 304, "reference_end": 582, "reference_sequence": "MKMRRKLIALSVAFGLIAPISGALMISCKNEKSNAEYQKELQNKWTELKNLLNSNSFKEVESKLDLSKLNALKEEYKTINSQSNVSTLKNFIAKINETAQDINAQISKLKNTNGNTNPENNSQNNLKVELEIATKKLDMLLSSNEGKNISIKEEAKKILNAANKALEKSNEQEYKNLIQKVNETIDKIEKEILNLEKGPKSKEFTKLKELREKLTNYLDNNFSEFAYKDLRAEIKELIQKQENVSIVSNEEEIKSAISQINDAFWIASIKKSILDKQFLYLENKPKSELDIKFLAAYDELSQALNTSIFENIETTNERNLLNKYIKTNLTKILDVEKQKYIEEVKNTISSLLNKYNNNINNNKDQIYELSRNIEQLNKLNQIENNYVIDKAKLLNLLNKANTIKNEGSFAEKAILLDELKDSVSEFLEFYLQLTHYLRIFFDKNNDIIKLLPADSQTKINDYLDEIKKITEKNSQTRGNEPTFDEYISAFKAQEEIFKIISSDLKSADVNILEKLTNNLKEIKKEKKNDYNLVVTSYLKEKIEETITKNESIIAKKSNVESQNIIETFALLQKLELEFAYINWTELNLIAKIKNKLELLIKINKIDSNEEAKILKPWTDSFKSSKKPEIFELENILKELSKSLKELINK" }, "paralog": false, "identity": 0.8903803131991052, "score": 756.0, "evalue": 1.17e-275, "description": "Internal stop codon at 505380, 506337, 506439. Nonsense mutation around 505380, 506337, 506439.", "psc": { "uniref90_id": "UniRef90_UPI000CD67922", "product": "P75 protein precursor", "uniref50_id": "UniRef50_Q9RLP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000CD67922", "UniRef:UniRef50_Q9RLP9" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RLP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RLP9" ], "product": "p75 protein" } }, "id": "LMJBGEANGM_883", "locus": "GCA013368015_00876" }, { "type": "cds", "sequence": "contig_1", "start": 506719, "stop": 507027, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D96F05" ], "nt": "ATGTTTGAAAAAACGAGAGAAGCTAGTGTTTATAAAATAAAAACATTCTTGCCCGATAAAACTACTCGTGATGAAAGAAAATTATATTTTGATAAAAGTCTTCCTTTATTTAAATTATTTAGCAAAGTAAGTGTAAATTTTAATACACTTTTAGGGGGAGCCAAAAAAACTAAAGGAATTTATGAACAAGATGGGAAATTAAGATTTGAAGGTTTTATTCAATTTTATGACTCAGAAATTTCCTGCTATAAATATTTTGGAACGGCAAATCCTATAACCATCGATGAAATAACAATTAAAGCCCAATAA", "aa": "MFEKTREASVYKIKTFLPDKTTRDERKLYFDKSLPLFKLFSKVSVNFNTLLGGAKKTKGIYEQDGKLRFEGFIQFYDSEISCYKYFGTANPITIDEITIKAQ", "aa_hexdigest": "bafe13e8131e2b00ea71cc264b7ab974", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D96F05", "query_cov": 0.8431372549019608, "subject_cov": 1.0, "identity": 0.872, "score": 152.0, "evalue": 2.28e-46, "valid": false, "uniref50_id": "UniRef50_UPI0034D96F05" }, "pscc": { "uniref50_id": "UniRef50_UPI0034D96F05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D96F05" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11901.58269999999, "isoelectric_point": 9.300612068176267 }, "id": "LMJBGEANGM_884", "locus": "GCA013368015_00877" }, { "type": "cds", "sequence": "contig_1", "start": 507142, "stop": 507774, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACATTAATTGATTCTGCAAAGGCAAAAAATGTTGATAATTCTGAAGCAAAAAAAGTATTAAATGCTACAAATGTTACTGAATCATCAAATATTTCAGACATTAAATCAAAAACAAAGAATATTGAAGATGCAATCAATTCATTAACTAAAAAAATTGATGAAGCTAAAAGCCATGAAGTTGTGCCACCAAGTGATGAATTTTTAAAAATTGAAGAAGAATTTCAAGACCAAAAGAAGCAAATTGAATCTAAACTAGATGAAATTTGCTCTCTAAAATTTAATAAGACATACAGCGAACAATTAATTAAATCAAATGAATCTATAAAGAGCGCAATTAATAAAAAAGTTAATAACGAAGTTTATGGTGAATATCCGGGAACTGAAGAAAGATTATATTATAGAAAAGCCTATAATGCAAGAAATAAAGAAGATTGTAAGAAATATATAAATCAACTTAAAAATATTTATAAAATAGTTGATATGTATGCTAATTATTCAAAAGAAATATTTAAATATATTGAATCAGAAGATGCAAAATTAAATGATGAAATAAAACGAAAGATAATGGAAGAAGATGTTATAGAGATTACCAAAGATAGCAGAAGCGAAATTGAATATTTACATTGA", "aa": "METLIDSAKAKNVDNSEAKKVLNATNVTESSNISDIKSKTKNIEDAINSLTKKIDEAKSHEVVPPSDEFLKIEEEFQDQKKQIESKLDEICSLKFNKTYSEQLIKSNESIKSAINKKVNNEVYGEYPGTEERLYYRKAYNARNKEDCKKYINQLKNIYKIVDMYANYSKEIFKYIESEDAKLNDEIKRKIMEEDVIEITKDSRSEIEYLH", "aa_hexdigest": "df793204e588780db4a37cfd202baccd", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24519.276600000034, "isoelectric_point": 5.399329185485839 }, "id": "LMJBGEANGM_885", "locus": "GCA013368015_00878" }, { "type": "cds", "sequence": "contig_1", "start": 507895, "stop": 508431, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGAGCACTTTTAGATTCAATAGACAATAAGATTAATAAACAAGACGAAGAAGAAAAATATAAAAATATTGAAGTTAAAGATAACGATTTAGTTAAAGACATTAAAAATAAGACTAATGAAATTTCAAAAGCAATTGAATCATTGAATAAAAAAATTAATGAACAAGAATTTGAACAAGCAAAACAAGATTTTGAAACTAAGAAACAAGAATTAAAAGCATTAATTGAATCTGCAAGGGCAAAAAATGTTGATAATTCTGAAGCACAAAATGTATTTGATACTACAAATGTTACTGAATCTTCAAATATTTCAGACATTAAATCAAAAACAAAGATTATTGAAGAAGCAATCAATTCATTGACTAAAAAAATTAATGAACAAGAAAATAAACAAGAATTTGAACAAGCAAAAAAAGATTTTGAAAAAATAAAAAAAGAACTACAAGAATTAATTGCAACCCCTGATGCAAACGAAGTAGGAAACATTGATGATATAACCAAAACCTTGAATGAAGCAAACATTGCCCAAGATTGA", "aa": "MRALLDSIDNKINKQDEEEKYKNIEVKDNDLVKDIKNKTNEISKAIESLNKKINEQEFEQAKQDFETKKQELKALIESARAKNVDNSEAQNVFDTTNVTESSNISDIKSKTKIIEEAINSLTKKINEQENKQEFEQAKKDFEKIKKELQELIATPDANEVGNIDDITKTLNEANIAQD", "aa_hexdigest": "b7f8b047e00744a0ff2e61c7c642fd36", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20431.431099999998, "isoelectric_point": 4.819742012023925 }, "id": "LMJBGEANGM_886", "locus": "GCA013368015_00879" }, { "type": "cds", "sequence": "contig_1", "start": 508837, "stop": 509022, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAAATTTATCATTACAAATTTTAGCGTTGTGTGTAGCAGCGGGCTCTGTGCCTGTTTTAGCATCTAAATGTACCGTAACTGTAAAAGTTAAAGAAAAGTTTAATGCTCTAAAATCACAACTTCAAAATTTAATTTCAAAACTAAGTGATGAAGAACAACAAGAACTTAATGAATGA", "aa": "MKKKNLSLQILALCVAAGSVPVLASKCTVTVKVKEKFNALKSQLQNLISKLSDEEQQELNE", "aa_hexdigest": "4a25dd07e169d330eef575d4d9523fbb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6700.840199999999, "isoelectric_point": 9.064206504821776 }, "id": "LMJBGEANGM_887", "locus": "GCA013368015_00880" }, { "type": "cds", "sequence": "contig_1", "start": 509255, "stop": 509803, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAATGATATTTGTAAAGAAGTAATTAAACAAGAATTTTTAGAAAAAAATTCAAATAACAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTATTGCCGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATAGGTTTTTGCTTTTTTTCATTTTTTGCTAATAATAAGAAAATAATTAAATCATTTGCAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCTTTAGACAAAGCAAGACAAGCATACATTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAGACAAAATGGGGATAAAAAAAATATCACAATACGATGCTGAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGA", "aa": "MEDQKDFNIIDSEIAAFGNVKKIFDENINNINDICKEVIKQEFLEKNSNNKRIEEAKKQYEIVRDSNERIIAEKKLKKISGRKFANCIIFILFFILIGFCFFSFFANNKKIIKSFAEFRNNEQLKIDEALDKARQAYIETFSLQTLEDIKNKILDKMGIKKISQYDAEVGDFSRSFIYDKNF", "aa_hexdigest": "bc05febee18a72d163927a6bb3feb77b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21474.452999999994, "isoelectric_point": 8.292777824401856 }, "id": "LMJBGEANGM_888", "locus": "GCA013368015_00881" }, { "type": "cds", "sequence": "contig_1", "start": 510227, "stop": 511135, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032", "UniRef:UniRef90_UPI00215DA032" ], "nt": "ATGAATCAAGAAAACATGCCGGTGTTTTCGATGATAAAAAATGGCAATTGGCTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAGGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAGTTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATGTCCAAAATTACCCTCTAAACATTTACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTCATAATGACCCAGAATCTTTGATAATATCAGCTACATTTGATTTGATTTCATATACAGCCAGAGACGCAATTGCAATTGAATATACACATGGTTACGCCGTTGAAGTACCATATGTGGCATATGATGAATTTAGAGAACATAAAGCAATAATTGTTGTTCCTTATAATATAAAAACAGAAAATGCCAAAATATTTGCAAATAGTCTTATCGCTAATAATATTTATGTAAGCGACGATCGCATAGATGAAGAATATAGCAAAATTATCCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAAGCTTATAGATGAATTATTTAGTCAGATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAATTAAAAAGCTTAATAATTTTTATTAA", "aa": "MNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIIECLLPIFNFISLVYTNKYIATENLEKTENRYEQDLVVKQNNNVQNYPLNIYSYYRDMTLYSGFYKFNKHADITPICEFKNLHNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLIKKLNNFY", "aa_hexdigest": "35e2c9532c81f8300b8e0b6895601529", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 509927, "stop": 511135, "observations": { "mutation": [ 509942, 509954 ], "stop": [ 509942, 509954 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA032", "query_cov": 1.0, "subject_cov": 0.6425531914893617, "identity": 0.9740000000000001, "score": 575.0, "evalue": 1.78e-204, "gene_start": 1, "gene_end": 302, "reference_start": 169, "reference_end": 470, "reference_sequence": "MGIKKISQYDAEVGDFSRSFIYDKNFWNFNSISQYKLRNSYIYDIFYTCKWMKNVVTKASITVTEEDANGNTYWETYWAYHTEPTPVVEYFRSLTIPSNYKQKLYFEIIENNRSEKEVKRLLKNEGFHLENKEVYQNFDFAFSDDQKIDFLTYFDVRTQQNLINYAQYMNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIVECLLPIFNFISLVYTNKYIATENLEKTGNRYEQDLVVKQNNNIQNCPLDIHSYYRDMTLYSGFYKFNKHADITPICEFKNLYNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLVKKLNNFY" }, "paralog": false, "identity": 0.9751243781094527, "score": 789.0, "evalue": 3.26e-291, "description": "Internal stop codon at 509942, 509954. Nonsense mutation around 509942, 509954.", "psc": { "uniref90_id": "UniRef90_UPI00215DA032", "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA032", "UniRef:UniRef50_UPI00215DA032" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35467.1385, "isoelectric_point": 4.91523151397705 }, "id": "LMJBGEANGM_889", "locus": "GCA013368015_00882" }, { "type": "cds", "sequence": "contig_1", "start": 511223, "stop": 511780, "strand": "+", "frame": 2, "gene": "lemA", "product": "Protein LemA", "db_xrefs": [ "COG:COG1704", "COG:M", "GO:0005886", "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_P43054", "UniRef:UniRef90_P43054" ], "nt": "ATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAACCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATCTTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATCGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATCAGAGGTGCTTTGAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACCGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGA", "aa": "MLFDSRTPQSSEGFKPNVDNSIKKPIPTGVEKFFFILFFILTIGIFYFVYVGRKNELMRDQNEIQNASSLIQAAEKRRRAVLIKMMDSLIGYKNFENETLTKITQYRSKLSNIDVDKTSPVELKSQIDSIRGALNFQFEQYPDLKASKLYLQFSTEISMQEDEIYATIRNYNMIATSFNSKIYTF", "aa_hexdigest": "181baf512e89f9176479d877cbeb4d28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_P43054", "query_cov": 1.0, "subject_cov": 0.8447488584474886, "identity": 0.995, "score": 357.0, "evalue": 3.14e-124, "valid": true, "gene": "lemA", "product": "Protein LemA", "uniref50_id": "UniRef50_P43054", "cog_id": "COG1704", "cog_category": "M", "kegg_orthology_id": "K03744", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P43054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43054" ], "product": "Protein LemA" }, "genes": [ "lemA" ], "id": "LMJBGEANGM_890", "locus": "GCA013368015_00883" }, { "type": "cds", "sequence": "contig_1", "start": 511968, "stop": 512102, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGCTCAATAGGGTTCAAGGGTTTGCACCACTTGCTATGA", "aa": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLL", "aa_hexdigest": "8d4f09ee56f4b74af8dfcccb6640f0f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5048.840599999998, "isoelectric_point": 6.81854190826416 }, "id": "LMJBGEANGM_891", "locus": "GCA013368015_00884" }, { "type": "cds", "sequence": "contig_1", "start": 512109, "stop": 512378, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "GTGGATAACTCAGTCGATGAAGCAATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAATAATGTAATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACTGGAATTCACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGTGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGATTACATGGTGTTGGTGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGA", "aa": "MDNSVDEAMAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEV", "aa_hexdigest": "fdcf49f4135e5c9de12a835c7dbf88a5", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 0.9887640449438202, "subject_cov": 0.9166666666666666, "identity": 0.602, "score": 113.0, "evalue": 6.4e-31, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 88, "aa_cov": 0.9550561797752809, "hmm_cov": 0.8181818181818182, "evalue": 7.924815484365216e-13, "score": 44.40375900268555, "start": 1, "stop": 85 } ], "seq_stats": { "molecular_weight": 9266.2869, "isoelectric_point": 5.17242794036865 }, "id": "LMJBGEANGM_892", "locus": "GCA013368015_00885" }, { "type": "cds", "sequence": "contig_1", "start": 512442, "stop": 513752, "strand": "+", "frame": 3, "gene": "gyrB", "product": "DNA gyrase subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P43053", "UniRef:UniRef90_P43053" ], "nt": "TTGGAAGTAATTGGAAATGTTTCTGAAGTTGAAACAGGAACAACAATTAAATTTCACCCTGACTATACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAGGGAGTTTTTAACGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATCGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACATACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATTGCCACAAATAAAATTCTTTCAGACTCACTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCAGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCAGCCGGAGGTTCTGCTAAAATGGGAAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGCACAGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCACACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCACTATATAAAATAACTAGCGGAAAAAATGTTGAATATGCATATAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGA", "aa": "MEVIGNVSEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQL", "aa_hexdigest": "bc75bad8e7b5d15584c7dc0d235ae5dd", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI000D1D9E0B", "query_cov": 0.8555045871559633, "subject_cov": 0.8923444976076556, "identity": 0.517, "score": 389.0, "evalue": 1.98e-129, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_F5VUB3" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 512142, "stop": 513914, "observations": { "mutation": [ 512376, 513750 ], "stop": [ 512376, 513750 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43053", "query_cov": 1.0, "subject_cov": 0.6728395061728395, "identity": 0.998, "score": 856.0, "evalue": 4.02e-310, "gene_start": 1, "gene_end": 436, "reference_start": 159, "reference_end": 594, "reference_sequence": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLLWEIVDNSVDEAMAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEVWVKRDGKLHYQQFRNGGIPVKPLEVIGNVSEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQLWETTMDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI" }, "paralog": false, "identity": 0.9966101694915255, "score": 1160.0, "evalue": 0.0, "description": "Internal stop codon at 512376, 513750. Nonsense mutation around 512376, 513750.", "psc": { "uniref90_id": "UniRef90_P43053", "gene": "gyrB", "product": "DNA gyrase subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P43053", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43053", "EC:5.6.2.2", "UniRef:UniRef50_P43053", "COG:COG0187", "COG:L", "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ] }, "pscc": { "uniref50_id": "UniRef50_P43053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43053" ], "product": "DNA gyrase subunit B" } }, "id": "LMJBGEANGM_893", "locus": "GCA013368015_00886" }, { "type": "cds", "sequence": "contig_1", "start": 513762, "stop": 513914, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTATTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAA", "aa": "MDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI", "aa_hexdigest": "21e2080d0bfa91ac8070ba9604ef6d73", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5754.417799999999, "isoelectric_point": 4.176893043518065 }, "id": "LMJBGEANGM_894", "locus": "GCA013368015_00887" }, { "type": "cds", "sequence": "contig_1", "start": 513916, "stop": 514101, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGCCTCTAACTAATCAAGGTTTCACAAACAAAGTATTTTACGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCGTTCTTATTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGA", "aa": "MLKPLTNQGFTNKVFYDDETNRFIKIKSYDGFNHKSDAFLLNNLDFCPKIFVDNKKELQTE", "aa_hexdigest": "beb625d91cc544b720c1c5a13551fe00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.125100000001, "isoelectric_point": 6.551284980773925 }, "id": "LMJBGEANGM_895", "locus": "GCA013368015_00888" }, { "type": "cds", "sequence": "contig_1", "start": 514132, "stop": 514356, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATGACATTTTAAAAACTATTGGAAAAAATTTAATCACTTTGCATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAAAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATTGACAATTCCGCCCCTGTCCATAATGACCTTTGA", "aa": "MTDDILKTIGKNLITLHNSKLKFYKENQIARRFNIYRKKISSLNRKIPILDKYYKKINLFLRNIDNSAPVHNDL", "aa_hexdigest": "19fc795c88b81a826147d5e7c4888cec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8862.315299999995, "isoelectric_point": 10.167453956604003 }, "id": "LMJBGEANGM_896", "locus": "GCA013368015_00889" }, { "type": "cds", "sequence": "contig_1", "start": 514417, "stop": 514572, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGATGTTCATTTTGACCTTGCATATTTTATTGAATCAAGCAATCTAGATAAAAAACAAGAAAAAGTGTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTAGTAAATGCATTAATTGTGCTATGA", "aa": "MGDVHFDLAYFIESSNLDKKQEKVFLDAYGDDFEPKYLFIHKILVNALIVL", "aa_hexdigest": "880f97a9a2dcf6c8cb45982de4c4fe6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5963.804499999998, "isoelectric_point": 4.7349950790405275 }, "id": "LMJBGEANGM_897", "locus": "GCA013368015_00890" }, { "type": "cds", "sequence": "contig_1", "start": 514667, "stop": 514870, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGATGATTTACATAGTAGCACATATATTGGAAAACTAGATGATGTTTGA", "aa": "MLKRDCCCGCKSAEFCTCKNKKSCIPNNLYEKIRNVFGYEVFEKLNNLRPYFDDLHSSTYIGKLDDV", "aa_hexdigest": "41eadcc39d14190c748f3075323eae69", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.973599999999, "isoelectric_point": 8.167257881164552 }, "id": "LMJBGEANGM_898", "locus": "GCA013368015_00891" }, { "type": "cds", "sequence": "contig_1", "start": 515297, "stop": 515605, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTGTATTTGATGATTTTATTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATTCCAAATCGCTAAGCCCTTTTGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCTCTTTGTGTTTACGAGGATGAAGATAGAACAATATGA", "aa": "MGIPKEDIIDFFKLDPSVFDDFIFLMRVYNEAMYLNDYSKNNSKSLSPFDSKSLYSNKDFLELNRFIVQKNHNNFDNKLNISKIEKFYFVPLCVYEDEDRTI", "aa_hexdigest": "36e512004936d33f465d66cfe0657b23", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12244.743599999987, "isoelectric_point": 4.881014442443848 }, "id": "LMJBGEANGM_899", "locus": "GCA013368015_00892" }, { "type": "cds", "sequence": "contig_1", "start": 516014, "stop": 516166, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTCACAGCATATTTATATAACAAATTATTAAACACTGATTTTAACGCCGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAA", "aa": "MICPKDFYKYRIIVHFTAYLYNKLLNTDFNAAKVNTKRINEIFEKLNIKD", "aa_hexdigest": "4d6d525a10d2f40f07be44431cd0d210", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6072.0634, "isoelectric_point": 9.416461753845216 }, "id": "LMJBGEANGM_900", "locus": "GCA013368015_00893" }, { "type": "cds", "sequence": "contig_1", "start": 516177, "stop": 516659, "strand": "+", "frame": 3, "gene": null, "product": "putative protein MHO_3790", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056", "UniRef:UniRef90_P43056" ], "nt": "ATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGATGTCGACAAGCAAGAAAAAAAATCAGTTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAGCGAACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAATAAAACTTATAAAGTAATTACCCTAACATCTAAAAGTCTTAATGTTTCTGATATTATGAGCGAAAGCGCAAGATACATGAATATTCCACAAAAATCATTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAATACCAACTTGTTAAAAAATACAAAAATTAATGAGACAAAGGTTACAAAAATAACTAATAGTAACCAAACTATTTTTAAATTAGATTTAGTTCAACTATGA", "aa": "MKKGKAKETKTIDVDKQEKKSVRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKVITLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLVQL", "aa_hexdigest": "08bf3ce434bae0487024e7a669fdb2b9", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 516177, "stop": 516962, "observations": { "mutation": [ 516657 ], "stop": [ 516657 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43056", "query_cov": 1.0, "subject_cov": 0.5970149253731343, "identity": 0.9940000000000001, "score": 291.0, "evalue": 1.36e-97, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKKGKAKETKTIDVDKQEKKSVRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKVITLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLIQLWNEINSKSNNKISEFDFLSNIESINNAMTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI" }, "paralog": false, "identity": 0.9923371647509579, "score": 505.0, "evalue": 1.66e-184, "description": "Internal stop codon at 516657. Nonsense mutation around 516657.", "psc": { "uniref90_id": "UniRef90_P43056", "product": "Uncharacterized protein MHO_3790", "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43056", "UniRef:UniRef50_P43056" ] }, "pscc": { "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056" ], "product": "Uncharacterized protein MHO_3790" } }, "id": "LMJBGEANGM_901", "locus": "GCA013368015_00894" }, { "type": "cds", "sequence": "contig_1", "start": 516738, "stop": 516983, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTACTCTTATATAAAAAATAACAAACTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAGTTCTTTGAAAATTTTGATGTTGAGATTCGTAAAGAAAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATTGAATTAAACGAATCATTAACCGAAATTAAAAAAATCACTTTTGATACTAAAATTACATTAAAATATGAAATATAA", "aa": "MTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI", "aa_hexdigest": "3dc4f00d8a053d96587fe4510e7deb2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9721.00909999999, "isoelectric_point": 7.864836692810059 }, "id": "LMJBGEANGM_902", "locus": "GCA013368015_00895" }, { "type": "cds", "sequence": "contig_1", "start": 517026, "stop": 517445, "strand": "-", "frame": 1, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGTTACAATACTTTGGAGCATTGAACGAAGCACAATTACGTAATATGCGTAGAGCAGTTGACCCTGCATACCATGGACAAGGAATTGAAGCAAAATCTGATTTACCTGCAAATGCATCAATTAATGATGTAAGAAGATTTATGTCAGCAAACTCAGCATTAGGTGTTGGTGACATTATGTCTGAATCAAAACGTTACCTAAATCTAGCTGGACAAGCAATTTCATACAATGCTGGTAAAGAAAAGATGCTATCTCTATACGACCAAGTACGTAAACACTTAGGACTATCAAGAAGAGACTTTGTTGAAAAAGAAAACCACAAATACATAAAAGAATTCTTTGACGTTCTATTAAAGAATAGTGCCTTACCTCTAGATACTTTAGAAAAAGTTACAAAGATTCACTACGGTATAAAATAA", "aa": "MLQYFGALNEAQLRNMRRAVDPAYHGQGIEAKSDLPANASINDVRRFMSANSALGVGDIMSESKRYLNLAGQAISYNAGKEKMLSLYDQVRKHLGLSRRDFVEKENHKYIKEFFDVLLKNSALPLDTLEKVTKIHYGIK", "aa_hexdigest": "18a64166ce19bbb7dabfe5108129b1e1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.9424460431654677, "subject_cov": 1.0234375, "identity": 0.611, "score": 164.0, "evalue": 4.8e-50, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "LMJBGEANGM_903", "locus": "GCA013368015_00896" }, { "type": "cds", "sequence": "contig_1", "start": 517719, "stop": 517826, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACACCACAACCAAATTTACTACGCACGTCAACACTTAGCAAGTCAAAACGGCGAAAATCTAGGTGCAAATGCATTTGACTTTACTTCATACGTTGAAGGTTGA", "aa": "MGHHNQIYYARQHLASQNGENLGANAFDFTSYVEG", "aa_hexdigest": "3ce943058666fa757fc121b9ebcdee3a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3941.1746000000003, "isoelectric_point": 5.727915382385254 }, "id": "LMJBGEANGM_904", "locus": "GCA013368015_00897" }, { "type": "cds", "sequence": "contig_1", "start": 517860, "stop": 518069, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCAACCATCAAGAACGGAAAATCATTCTTCGGTAAAACTGTTCCTGACTACAAAGCAGTAACATATCTATATGAAAGACGTGATCAAGAAGGTGTTGGAGCATACAACTCAGATACACGTATTTTGGATGCAAACGGAAATGTAACTTCTGTTCAAGGTGCATTTAACTTCAACTGTGACCCATACTACTCATTGCCAAAATGA", "aa": "MKSTIKNGKSFFGKTVPDYKAVTYLYERRDQEGVGAYNSDTRILDANGNVTSVQGAFNFNCDPYYSLPK", "aa_hexdigest": "39ee82c016e2bc1fcb674a55993c58f5", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7752.532500000003, "isoelectric_point": 8.75869083404541 }, "id": "LMJBGEANGM_905", "locus": "GCA013368015_00898" }, { "type": "cds", "sequence": "contig_1", "start": 518415, "stop": 518747, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAGAAAATGTTTACAAAGAAACAGAAAATACAATATCAGTTTCAAAACAAGAAGACGGAAAACTTATTGAAAGATCAGTTTATGGTTTAGGTTTTACCAAATTTGACTTAGAAGCTAAGGGTGTTGGATTCTCACAAGTTGGTGGCGACGAAACTGAAAGTGGAAAGACCATTTATGACCAAATCACAAAAATGGCAACCACATCAGAAGACACTCCAAACGATGTATATCAATCAGGATACAAAACATCAAAAGGTGTTTCAAAGAACATGACTGCTATTGCATCAGAAGTTGCTAAATTAATAGCAGGCGAAAATGGAGCATGA", "aa": "MYKENVYKETENTISVSKQEDGKLIERSVYGLGFTKFDLEAKGVGFSQVGGDETESGKTIYDQITKMATTSEDTPNDVYQSGYKTSKGVSKNMTAIASEVAKLIAGENGA", "aa_hexdigest": "c14f7e1eb6119632524ff80e19602fbc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11907.04139999999, "isoelectric_point": 4.849070930480956 }, "id": "LMJBGEANGM_906", "locus": "GCA013368015_00899" }, { "type": "tRNA", "sequence": "contig_1", "start": 520021, "stop": 520109, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 65.9, "nt": "GGATAGTTGCTCGAGAGGCTGAAGAGGTTAGTCTCGAAAACTAATAATGTTTTTAACATTCAAGGGTTCAAATCCCTTACTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 520096, 520098 ], "id": "LMJBGEANGM_26", "locus": "GCA013368015_00900" }, { "type": "cds", "sequence": "contig_1", "start": 520369, "stop": 521655, "strand": "+", "frame": 1, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_Q6Y8Q8" ], "nt": "ATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGTCTAAGAAAAGAAAGGCAAGGAAATGCTTATTTACAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATACGTTGGTTTGCCAGAGGGGCAAATTGGCAATAGCGAAGTAGGACATTTAAATATTGGCGCAGGTAGAGTTGTATATACGGGATTATCTTTAATTAATAAGGCAATTCAAAATGATACTTTTAAGGATAATGAAATTTTAAATGATGTCATAGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATCCTGAAATTAGCGCATAGTAAAGGATTAAAAAACGTAAGTGTTCATGTGTTTGGTGATGGAAGAGATGTAAAACCACAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGGTTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAAGCATATGATGCTATTTTAGGGCAATCAAAAAATGTTATTAAAAACATTGATGACTACATTGAAAGTCAATATAAAAAAGGAATATTTGACGAATTTTTTGAGCCTGCTCAATTAAAAGACGGCGCATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGATAGAGCAAGACAACTAAGCCATATGTTTATTGGATCAAGCTTATACACATACAAACCAAAAAACCAAGTACAAATTGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAAATAGCCTTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAGTAATGACATTAAACAATTAAGACTAGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGGGGAGTTGACATCGAATATAAAAATGAAAATAGAATTCTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCTAAAGAAATTACAGATTCACTATTGAACAACGTTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGTCATACCGGTAATTTGAACGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTTCAAAGAATATTAGAATATGTATCATTAAATCATGAAAATGTAACTTGA", "aa": "MKKIILTIIDGLGLRKERQGNAYLQAKHPCFDYLFSMCPNSVLQASGQYVGLPEGQIGNSEVGHLNIGAGRVVYTGLSLINKAIQNDTFKDNEILNDVIDKTIKNNTTLHVMGLLSNGGVHSLDLHLFEILKLAHSKGLKNVSVHVFGDGRDVKPQSIKNSLETLKDLCQKFGYKISSISGRFYAMDRDSIFSRNQEAYDAILGQSKNVIKNIDDYIESQYKKGIFDEFFEPAQLKDGAFVKNGDSIIFFNFRPDRARQLSHMFIGSSLYTYKPKNQVQIDNFVSLMKYEGINSKIAFKEMEVVNPLGKVLESNDIKQLRLAETQKYAHVTFFFDGGVDIEYKNENRILVDSLKVDSFADYPHMSAKEITDSLLNNVEKNDFIIMNYANPDMVGHTGNLNATIEAIEFLDSQFQRILEYVSLNHENVT", "aa_hexdigest": "82861c24a3489af79ac400e9bc59a32e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q6Y8Q8", "query_cov": 1.0, "subject_cov": 0.8577154308617234, "identity": 0.986, "score": 840.0, "evalue": 2.89e-306, "valid": true, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "LMJBGEANGM_907", "locus": "GCA013368015_00901" }, { "type": "cds", "sequence": "contig_1", "start": 521740, "stop": 521868, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTGCACAGATAAAAATGTTAATTTAGAAAATGGTAGTCTTTGTGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAACAAACCAAAAGAAATGACTGGAAAATCATTACTTAAATAA", "aa": "MFICTDKNVNLENGSLCDVAPTILDYLKINKPKEMTGKSLLK", "aa_hexdigest": "3b357b54df9d82f8863eb3d8e8dd0ac8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4699.5321, "isoelectric_point": 7.761273384094238 }, "id": "LMJBGEANGM_908", "locus": "GCA013368015_00902" }, { "type": "cds", "sequence": "contig_1", "start": 521910, "stop": 522398, "strand": "-", "frame": 1, "gene": null, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "nt": "TTGCAAGATACATTTCTATTTAAAACAACAATTTTAGAAAATCTAAGATATGCTAATCCTAGTGCAAGCAATGAAGAAATCATTAATGCTGCCAAAATTACAAAGGCCGATAATTTTATAAGACAACTTGAACATGGTTACGAAGAAATAGTTGAAGAAGGCGGAAGCAATTTTTCACAAGGTGAAAGACAGATGCTTGCAATCACAAGAGCAATAATTGCTAATAAAAATATTTTGATATTAGACGAAGCAACATCTAATGTAGATACAAGAACAGAAAAGAATATTCAAGATGCAATGTTAGAGTTAATGAAAGGAAAAACTTCATTTGTTATTGCTCATAGACTTTCAACTATAGTAAATGCTAGCCAAATTTTAGTTGTTAAAGATGGAAAAATTATTGAACAAGGTACACACAAAGAACTATTGACTAAAGGTAGTTTTTATGAAAAGCTATATCATTCATCTTTTAGTGAAGAAAATGAATAA", "aa": "MQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE", "aa_hexdigest": "c4f9e9a000d7b0695385d06c1f095888", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZZ8", "query_cov": 0.9567901234567902, "subject_cov": 1.0, "identity": 0.735, "score": 229.0, "evalue": 5.29e-75, "valid": false, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "uniref50_id": "UniRef50_A0A3B0NZZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component" }, "genes": [], "id": "LMJBGEANGM_909", "locus": "GCA013368015_00903" }, { "type": "cds", "sequence": "contig_1", "start": 522420, "stop": 523481, "strand": "-", "frame": 1, "gene": "mdlB", "product": "ABC transporter ATP binding protein", "db_xrefs": [ "COG:COG1132", "COG:V", "SO:0001217", "UniRef:UniRef50_A0A448ZXF5", "UniRef:UniRef90_D1J8H0" ], "nt": "TTGATCTTAGGCTATATTTTAGAAAAAGTCTTTTCATTTATATGTTCAGCAATGGCAGTCAAGGCAGGAATTTATGGCTCAGAAATAATGCGGAGTAAGGCTTATAAATCAATAATGAAAATGCCAATCCAATTTTTTGATACTGTTAGCTCGGGAGAATTAATGTCAATATTGTCAAATGATATTGATAATGTTTCATATGGAATATGTGGAACCTTAAACTCGTTATTAGCAGCGATATTTAGTGCTTTAACATCGCTTGGATTTATGTTTTACTATTCTACATATTTAACACTTATATATATTGCCTTATTTCCTGTTTTGTTGATATTGGCTTTTGTTCTAATTAAAAAAGCAATTCCATTATTTGGCAAGACTCAAAGAAATATAGGTTTATTGAATGGTTTTGTTGAAGAAAATTTGGCAGCACACCAATTAATAAATTCATTGAATAGTCAACAAATTATTAACGAACAATTTGATTTATATAATGAAAAATTATATAAACCTTCATTAAAAGCAAATATATATGCCGGAATAGTTTATCCATATGGAAATGCAATAACATTCATAATGCAATTAATAATAATTTTTGTTGGCGGAGCATTTGCATTGTCTGGAATTGGCACTGGCTCAAATAGTAATTTTACAGTCGGTACATTGACATCATTTATTTTGTATATAAAAATTACTTCTAGTCAAATAATTAAAAATTTTGAAAATATTTCAATGATTCAAACAGCAACAATATCAACAACAAGAGTATTAAATTTAATTAGAATGCATCCGGACGTTGATGAAAGCAAACTTGCTACAATATCAAATGTGAATGGTGATATTGAATTTAAAAATGTAAGTTTTTCATATGATAATTCTTTAAATAATTTACAATTGAAAAATATTTCATTCAAAGCAAAAAAAGGTCAAGTATTTGCTATTGTAGGACCTACCGGGGCAGGCAAAACTACAATAATAAATTTACTAAGCAAATTTTATTTGCCTATTGAAGGGGAAATAAAAATTGATGATTATAAATCAAGCGAAATAAATGAATCTAGTTGA", "aa": "MILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVLIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPDVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDYKSSEINESS", "aa_hexdigest": "1d442b7a57eb954a8449fa3a0269c5d7", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "both", "pseudogene": { "start": 522117, "stop": 523781, "observations": { "mutation": [ 522422, 523532 ], "stop": [ 522422, 523532 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H0", "query_cov": 1.0, "subject_cov": 0.5648, "identity": 0.992, "score": 651.0, "evalue": 5.08e-231, "gene_start": 1, "gene_end": 353, "reference_start": 103, "reference_end": 455, "reference_sequence": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKFWNSNSNGLFEFGIAIGILILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVFIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDYKSSEINESSWRNQISIVLQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE" }, "paralog": false, "identity": 0.9891696750902527, "score": 1051.0, "evalue": 0.0, "description": "Internal stop codon at 522422, 523532. Nonsense mutation around 522422, 523532.", "psc": { "uniref90_id": "UniRef90_D1J8H0", "gene": "MdlB", "product": "ABC transporter ATP binding protein", "uniref50_id": "UniRef50_A0A448ZXF5", "cog_id": "COG1132", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H0", "UniRef:UniRef50_A0A448ZXF5", "COG:COG1132", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXF5" ], "product": "ABC-type multidrug/protein/lipid transport system ATPase component" } }, "id": "LMJBGEANGM_910", "locus": "GCA013368015_00904" }, { "type": "cds", "sequence": "contig_1", "start": 523530, "stop": 523787, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGGGATATTCTTGATCCGCTAAAAGTTGATATTGATTTAAAAAAAGTATCAAAATTTGAAAGAAAAGCATTTCAAAAAAAACAACACGCAATAAAATCAGAAAGTTTTAAAGAACTAATTAAATATTTAAATTACAAACGTGGATATGTTTTTTTAATAATAATAATTACATTAATTTCTTCATTGTGTTTAACTGCTACCACATATGGTTTGGGTTATATTATCGACCATTATTTAAACTTTAAGTTTTGA", "aa": "MNRDILDPLKVDIDLKKVSKFERKAFQKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTATTYGLGYIIDHYLNFKF", "aa_hexdigest": "708c20e7849d6ceb900a3430302fa757", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10064.849799999989, "isoelectric_point": 9.607481575012208 }, "id": "LMJBGEANGM_911", "locus": "GCA013368015_00905" }, { "type": "cds", "sequence": "contig_1", "start": 523780, "stop": 524682, "strand": "-", "frame": 3, "gene": null, "product": "Multidrug ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "nt": "ATGAATTACGTTATGACTGTTACATTTGGAGTTGTTGCTTCATCATTCACTACATTTAATATTTTTAAGGCAAATGTATCCTCAAAACGTATTTTTGAAATTATGAACAAAAAGCCAGATATTGCAAAAATCAAATCTGATAAGCTAATTGTTAATGGAGAAATTGAATTTAGCCATGTATCTTTTAAATATTATGAATCTGCCAAATCTAATGTTTTAGAAGATATTTCATTTACTTTAAAGCCTGGCAAGGTGTTAGGCATAATTGGCCCTACTGGCTCTGGAAAATCAACTATTGCAAAGTTATTAAATTTAGATTTTAAAACTCAAGAAGGATTAGTAACAATTGATGGGCATAATATTCAAGAAATTGATACAGACTCATTGAGAAGGAATATCTCCCACGTTTATCAAAACCCTTGCTTATTAAGTGGCACAATAAAAAGCAATTTATTGCTTGCTAAACCCAATGCTACTGACGAAGAGATTGAATTAGCTGCAAAAAATGGTTGTGCCTTTGAATATATCAATAAGTTTAAGGAAAAATTTGAACATGTTGTTGAACAAAAAGGCGCAAATTTATCAGGCGGTCAAAAACAAAGATTATCTATTGCCCAAGGTTTGATCAAACGACCTAAAATATTGATTTTAGATGATTCTACTTCAGCATTGGATGCTAGAACAGAAGCTATGGTAAGAAACAATATTAAAGAAGAATTTAAATATGAAAAAATATCATTAGTTATTATTGCCCAAAAAATTTCAGCAATTATTGATGCAGATGAAATTTTAGTATTAAACCAAGGAAAAATTATTGATCGTGGCAATCATGAGGAATTAATTTCTAGAGATGGTCTATATAAAGAAATTGCATATTCACAGTTAGGGGGTAATAATGAATAG", "aa": "MNYVMTVTFGVVASSFTTFNIFKANVSSKRIFEIMNKKPDIAKIKSDKLIVNGEIEFSHVSFKYYESAKSNVLEDISFTLKPGKVLGIIGPTGSGKSTIAKLLNLDFKTQEGLVTIDGHNIQEIDTDSLRRNISHVYQNPCLLSGTIKSNLLLAKPNATDEEIELAAKNGCAFEYINKFKEKFEHVVEQKGANLSGGQKQRLSIAQGLIKRPKILILDDSTSALDARTEAMVRNNIKEEFKYEKISLVIIAQKISAIIDADEILVLNQGKIIDRGNHEELISRDGLYKEIAYSQLGGNNE", "aa_hexdigest": "5d5131dbba2a4eb8211e5e41815dc798", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031CC58F0", "query_cov": 0.8, "subject_cov": 0.9876543209876543, "identity": 0.504, "score": 215.0, "evalue": 8.2e-66, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3D3WBZ0" }, "pscc": { "uniref50_id": "UniRef50_A0A3D3WBZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "product": "Multidrug ABC transporter ATP-binding protein" }, "genes": [], "id": "LMJBGEANGM_912", "locus": "GCA013368015_00906" }, { "type": "cds", "sequence": "contig_1", "start": 525244, "stop": 525603, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter transmembrane domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8", "UniRef:UniRef90_UPI00241122C8" ], "nt": "ATGTTTAAACTTTATAAATTAATGGCCAACAAATTCAAATGTTTGTCATTTTTAACTATATCTTTAACATTGCTACAAGTAGTTTCATTTTTAGTATTGCCAATATTATTGGGACAATTGACAAGGTTAATTGGTGAAAATGCATATTTAATTCAAAATAATTTGTCAACAAACAGACCAATAACAATTGAAATTCTAAGAATTAATTTTTTATGTCAAAGCCACCAGAGTGCATTAATGCATTTGGGGGGATATTTTGCATTGTTTTTAATAATAGGAACTATTTCAGCAATGTGTGCTTCATTGCTTGCATCATATGTGTCTCAAGCAGGATCAAAACAAATAAGAAGTTGTTTATGA", "aa": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCL", "aa_hexdigest": "3356d1856d119451e55003d5e2b1adf3", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 524943, "stop": 525603, "observations": { "insertion": [ 524979 ], "mutation": [ 524994, 525246 ], "stop": [ 524994, 525246 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241122C8", "query_cov": 1.0, "subject_cov": 0.5265486725663717, "identity": 1.0, "score": 216.0, "evalue": 1.44e-69, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCLWKHLGELSQKDIEAFSNAKILTRFTIDISRIQTGLMSFLRMLIIGPFNLVLGLVFALLTNLQLSMIFLVVIPLLTLTMVISGVIWNPIQKKNKKCMTKLTLKVEKIF" }, "paralog": false, "identity": 0.9908675799086758, "score": 405.0, "evalue": 2.01e-146, "description": "Internal stop codon at 524994, 525246. Frameshift due to insertion around 524979. Nonsense mutation around 524994, 525246.", "psc": { "uniref90_id": "UniRef90_UPI00241122C8", "product": "ABC transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241122C8", "UniRef:UniRef50_UPI00241122C8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8" ], "product": "ABC transporter transmembrane domain-containing protein" } }, "id": "LMJBGEANGM_913", "locus": "GCA013368015_00907" }, { "type": "cds", "sequence": "contig_1", "start": 525652, "stop": 526065, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATGAAGAATCAGGATTATTAGGAGTTTCAAAAATTTCTTCTGATATTCGTGACGTTGAAGAAGCTAAAAAAACAAATGAAAGAGCAAAATTTGCAATAGATTTATATGTAAGAAAAATAGCAGATTATTTAATTAATTACATGAATAAGATTGAAGGAAAAATTGATGCTATTGTTTTTACGGCGGGTGTGGGTGAAAATGATGAATATATTCGCCAAGCAGTTGTTGATAAAATTCATTTAGTTAAATTAGAAATTGATAGTGAATTAAATAAAGGAAGAGATTTTGATTCATACAAATTAATTTCAACTAAAAACTCTTCAATTCCTATTTATATAATAAGGGCAGATGAAGAACTTTATATCGCAAAAGAAGTTAAACAATTTTTTGATGATAATATAAGGTAA", "aa": "MLNEESGLLGVSKISSDIRDVEEAKKTNERAKFAIDLYVRKIADYLINYMNKIEGKIDAIVFTAGVGENDEYIRQAVVDKIHLVKLEIDSELNKGRDFDSYKLISTKNSSIPIYIIRADEELYIAKEVKQFFDDNIR", "aa_hexdigest": "cd478da426eb6890326af4751732ec2f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15753.760799999985, "isoelectric_point": 5.012255668640137 }, "id": "LMJBGEANGM_914", "locus": "GCA013368015_00908" }, { "type": "cds", "sequence": "contig_1", "start": 526087, "stop": 526431, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTCCACTTTGATACTTCATTCCATAAAACCCTTCCAAAAGAAGCATATACTTATGCAATTGATGCAAATATAACCGATGAATTAAATGTTAGAAAATACGGTTTCCATGGCTTGAATCACCACTTTATTACATTAAAATTGCAAGAAATTTTAAATAAAGACAAAGTTAATTTTGTTAATTTGCACATCGGAAATGGCGCAAGTTTAGCAGCCATTCAAGATAGTAAATCAATTGATACCTCAATGGGATTTACACCACTAGCGGGAGTTATGATGGGCACTCGTTCTGGTGATGTTGATTGTTCAATTATTCCATATATTATGAAACATAAAAATTGA", "aa": "MTFHFDTSFHKTLPKEAYTYAIDANITDELNVRKYGFHGLNHHFITLKLQEILNKDKVNFVNLHIGNGASLAAIQDSKSIDTSMGFTPLAGVMMGTRSGDVDCSIIPYIMKHKN", "aa_hexdigest": "bf9ea4f0f79cb2cb311df2ef9af91b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12773.492099999985, "isoelectric_point": 7.055731010437011 }, "id": "LMJBGEANGM_915", "locus": "GCA013368015_00909" }, { "type": "rRNA", "sequence": "contig_1", "start": 526936, "stop": 527040, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.3e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "LMJBGEANGM_38", "locus": "GCA013368015_00910" }, { "type": "cds", "sequence": "contig_1", "start": 527142, "stop": 527339, "strand": "-", "frame": 1, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26", "UniRef:UniRef90_D1J8H3" ], "nt": "ATGAAGTTCAATTTTTTTCCAATGGTGTTAGATCTTCATGGTTATGATGTAAACTCTGCAACAGCAGCAATTTTAAACGCTTTGTATGAATTTAAACAAGATGAATATAATAATTACTTTGATATCATTGTAGGCATTGGTAGTGGGATTATTCGCCAAATAACAGAAGACTTATTAGACAAAGAAGGATATTATTGA", "aa": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFKQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYY", "aa_hexdigest": "ea0ebfb1e6a408a77567f0648b2a77c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 527094, "stop": 527339, "observations": { "mutation": [ 527144 ], "stop": [ 527144 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H3", "query_cov": 1.0, "subject_cov": 0.7926829268292683, "identity": 0.985, "score": 135.0, "evalue": 2.25e-40, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFEQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYYWKNIGNSLAKIRVFKKI" }, "paralog": false, "identity": 0.9753086419753086, "score": 160.0, "evalue": 6.08e-53, "description": "Internal stop codon at 527144. Nonsense mutation around 527144.", "psc": { "uniref90_id": "UniRef90_D1J8H3", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H3", "UniRef:UniRef50_B3PN26" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26" ], "product": "Smr domain-containing protein" } }, "id": "LMJBGEANGM_916", "locus": "GCA013368015_00911" }, { "type": "cds", "sequence": "contig_1", "start": 527340, "stop": 528203, "strand": "-", "frame": 1, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872", "KEGG:K10563", "SO:0001217", "UniParc:UPI001F517007", "UniRef:UniRef100_UPI001F517007", "UniRef:UniRef50_A0A9Q9F2U2", "UniRef:UniRef90_A0A454C9Y4" ], "nt": "ATGAGTAATGTCATTCGAAAGTATTATAATTTTACTATGCCTGAATTACCTGAAGTTAGGGTTGTTGTTAAAGCCCTTAATAATACTATATTAAATAAAAAAATAACAAATTTGATTATATACAAACCCAAAATTTTTAAAGAGTTTAGCCCTCAATATTTCATTTCAATTTTAAGAGATAAAAAAATTGAAAAAATAGATAACATTGGCAAACATATAATTTTCTTTTTATCTGAAAAATTAGTTTTACTTTCTCATTTAAGAATGGAGGGAAAATATCGTTATTATTTAAACCCTCCAAAAGAAATTGATAAGCACTTAGTTGCTAGATTTGTTTTTAGCGACAACAGCGAATTGCACTTTTTAGATAAACGTTTATTTGGCACATATATGCTAAGAAACTTAGAAAATTATAACAAAATTCCTCCAATTTCTTTGCTTGGACCTGAGCCAAAGGATATTGACATTGAAGCATTATTTAAAAAAATAAAAAATTCAAAAATGCCCATTAAAACAAAATTACTTGATCAAAGTTTTGTTGCTGGTATAGGAAATATTTACGCTGACGAAGCATTGTTTGCTTCCAAAATTCATCCTCTTTCAAAAGCTTCTGATTTATCACTAGATCAACTTAATGACATAATAAAAAATGCAAATGAAATAATGGAAAAAAGTTATGAATTAGGTGGAACAACGTTATTTTCTTATGAATCATTAAATAAACAAGAAGGAAAATATCAAGACTTTTTAAATATTCATAGTGATACTATAAAAAAATGTAAGGTTTGTGGTCAAAAAACACTTAAATTAAAAGTTAACCAACGTGGTACATATGTTTGCCCTAATTGTCAGAAGGTATATTAA", "aa": "MSNVIRKYYNFTMPELPEVRVVVKALNNTILNKKITNLIIYKPKIFKEFSPQYFISILRDKKIEKIDNIGKHIIFFLSEKLVLLSHLRMEGKYRYYLNPPKEIDKHLVARFVFSDNSELHFLDKRLFGTYMLRNLENYNKIPPISLLGPEPKDIDIEALFKKIKNSKMPIKTKLLDQSFVAGIGNIYADEALFASKIHPLSKASDLSLDQLNDIIKNANEIMEKSYELGGTTLFSYESLNKQEGKYQDFLNIHSDTIKKCKVCGQKTLKLKVNQRGTYVCPNCQKVY", "aa_hexdigest": "bfee8f6a5f54a0dbf20f69775b279034", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001F517007", "uniref100_id": "UniRef100_UPI001F517007", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F517007", "UniRef:UniRef100_UPI001F517007" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F517007", "uniref90_id": "UniRef90_A0A454C9Y4" }, "psc": { "uniref90_id": "UniRef90_A0A454C9Y4", "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A9Q9F2U2", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2U2" ], "product": "DNA-(apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "LMJBGEANGM_917", "locus": "GCA013368015_00912" }, { "type": "cds", "sequence": "contig_1", "start": 528245, "stop": 528421, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGTGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGA", "aa": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKE", "aa_hexdigest": "fffbfe4be100210b6dc72de2222d76bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6619.7276, "isoelectric_point": 9.629852104187012 }, "id": "LMJBGEANGM_918", "locus": "GCA013368015_00913" }, { "type": "cds", "sequence": "contig_1", "start": 528500, "stop": 528712, "strand": "+", "frame": 2, "gene": "tsaC", "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "COG:COG0009", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A077L684", "UniRef:UniRef90_D1J8H5" ], "nt": "ATGCCAAACCAAAAAAAATTGCTTGAATATATAGAAGTAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAATGCACCTGTATGCAAAACTATTGAAAATGCAAAGCAATTATTCCCCGAAGTTACTAATGTATATAATTTTGGTCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAA", "aa": "MPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK", "aa_hexdigest": "31e42a5462e277f60c3012de9ffa43e2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaC" ], "truncated": "5-prime", "pseudogene": { "start": 528245, "stop": 528712, "observations": { "mutation": [ 528419 ], "stop": [ 528419 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H5", "query_cov": 1.0, "subject_cov": 0.45161290322580644, "identity": 1.0, "score": 147.0, "evalue": 6.82e-44, "gene_start": 1, "gene_end": 70, "reference_start": 86, "reference_end": 155, "reference_sequence": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKEWNSQADEFAKKYWPGATTIVVNNQGFRMPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK" }, "paralog": false, "identity": 0.9935483870967742, "score": 308.0, "evalue": 8.2e-110, "description": "Internal stop codon at 528419. Nonsense mutation around 528419.", "psc": { "uniref90_id": "UniRef90_D1J8H5", "gene": "TsaC", "product": "L-threonylcarbamoyladenylate synthase", "uniref50_id": "UniRef50_A0A077L684", "cog_id": "COG0009", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H5", "UniRef:UniRef50_A0A077L684", "COG:COG0009", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L684" ], "product": "L-threonylcarbamoyladenylate synthase" } }, "id": "LMJBGEANGM_919", "locus": "GCA013368015_00914" }, { "type": "cds", "sequence": "contig_1", "start": 528714, "stop": 529109, "strand": "+", "frame": 3, "gene": "rpiB", "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "COG:COG0698", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8H6", "UniRef:UniRef90_D1J8H6" ], "nt": "ATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGACGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCAGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGCGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAGTTGAAAAAATTTTTCATAGCTGA", "aa": "MKTTVKITSDHAGYDAKINLAKRLENEGYNVELFGSKTNNNSDSYADAAIELVNEIHKDGNPDENKNVKYVAFCGSGIGIEMTLNKFKGIRAARVTNENESHLAKLHNNANVLCMGGRLLEGNEVEKIFHS", "aa_hexdigest": "56578a33b1ae849663dc0a78aefbb2c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8H6", "query_cov": 1.0, "subject_cov": 0.8451612903225807, "identity": 1.0, "score": 265.0, "evalue": 2.05e-89, "valid": true, "gene": "RpiB", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_D1J8H6", "cog_id": "COG0698", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_D1J8H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8H6" ], "product": "Ribose-5-phosphate isomerase" }, "genes": [ "rpiB" ], "id": "LMJBGEANGM_920", "locus": "GCA013368015_00915" }, { "type": "cds", "sequence": "contig_1", "start": 529213, "stop": 529404, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTAGAAGTATTAAATCTTCCAGATACATTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCAGCAGCTAAAATTTCTTTCTTAATCATATTTTGTTCCAAGAATAAACAATATGTTTCAATTTTAACCGTTTCTGTATTGGTATAA", "aa": "MSLFLFLYIFYFSILEVLNLPDTFSTFDEVIHALLSAAKISFLIIFCSKNKQYVSILTVSVLV", "aa_hexdigest": "6c0c4c9537bfc19bcab723c3f5889836", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7227.5594, "isoelectric_point": 5.4644666671752935 }, "id": "LMJBGEANGM_921", "locus": "GCA013368015_00916" }, { "type": "cds", "sequence": "contig_1", "start": 530238, "stop": 530426, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6", "UniRef:UniRef90_UPI00036493F6" ], "nt": "ATGGTCATGATTATTATTTCGGCCGTTTTATACAATATTGGTGTTGCAACATTCTTGGCTAAGGCTGCCACTGTTGCAACTGGGTTATCTGCATTGGTTCAAGCCATTACTCTAACATTAACTCAAACCGCACCATATTTTGCTTATATTTATATAGCAATGAATTTACCTTTTGTAATTGCATTTTGA", "aa": "MVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAF", "aa_hexdigest": "e9ecd32b7bb53b225037a9f72ff45509", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 530207, "stop": 530576, "observations": { "insertion": [ 530234 ], "mutation": [ 530240, 530447 ], "stop": [ 530215, 530240, 530447 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00036493F6", "query_cov": 1.0, "subject_cov": 0.5081967213114754, "identity": 1.0, "score": 103.0, "evalue": 2.01e-27, "gene_start": 1, "gene_end": 62, "reference_start": 51, "reference_end": 112, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKMVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAFWKKILDYLWY" }, "paralog": false, "identity": 0.9754098360655737, "score": 221.0, "evalue": 3.21e-76, "description": "Internal stop codon at 530215, 530240, 530447. Frameshift due to insertion around 530234. Nonsense mutation around 530240, 530447.", "psc": { "uniref90_id": "UniRef90_UPI00036493F6", "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00036493F6", "UniRef:UniRef50_UPI00036493F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6600.926899999999, "isoelectric_point": 8.138311576843265 }, "id": "LMJBGEANGM_922", "locus": "GCA013368015_00917" }, { "type": "cds", "sequence": "contig_1", "start": 530445, "stop": 530576, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B", "UniRef:UniRef90_UPI002410BF5B" ], "nt": "ATGAAACATACAGCTGAGGAACTTGCTGAGATTAAAGAACGTAAACGTTTAAAGAAAGAAACCAAAAAAGAAAAGGGATCAGTCGGCAAAATTGACATAAATCCTTATGGGGTAAATTTCTTTAATATTTGA", "aa": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNI", "aa_hexdigest": "3bbac7b9d353dab09e5c0c372e3c1fba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 530365, "stop": 530576, "observations": { "deletion": [ 530429 ], "mutation": [ 530447 ], "stop": [ 530447 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410BF5B", "query_cov": 1.0, "subject_cov": 0.6142857142857143, "identity": 1.0, "score": 84.0, "evalue": 1.76e-20, "gene_start": 1, "gene_end": 43, "reference_start": 1, "reference_end": 43, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKNGHDYYFSRFIQYWCCNILG" }, "paralog": false, "identity": 0.9714285714285714, "score": 141.0, "evalue": 7.69e-46, "description": "Internal stop codon at 530447. Frameshift due to deletion around 530429. Nonsense mutation around 530447.", "psc": { "uniref90_id": "UniRef90_UPI002410BF5B", "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410BF5B", "UniRef:UniRef50_UPI002410BF5B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5018.7903000000015, "isoelectric_point": 9.630819129943848 }, "id": "LMJBGEANGM_923", "locus": "GCA013368015_00918" }, { "type": "cds", "sequence": "contig_1", "start": 530743, "stop": 531012, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGGACTGCAATTTACTTATTAGTATTTTCAATGTTAGTAAATAAGTTCTATCCAAAATACAAGAAAGTAAGAATTGATATTTACACCACAAAACCAGAACTAGTTGCTGAAAGATTTAAACAAATTGGATATGTTCACTCATTCAATGTATTCAACGGATTTGGTGGATATAACCATCAAGATTTTGGAAAAATTGAAACAATTGCCCTATATTTAGAAAAAGAATCAATTTTAGAAGAAGTTAGAAGCGTTGATAAAAAAGCCTGA", "aa": "MGTAIYLLVFSMLVNKFYPKYKKVRIDIYTTKPELVAERFKQIGYVHSFNVFNGFGGYNHQDFGKIETIALYLEKESILEEVRSVDKKA", "aa_hexdigest": "5fb240ebea4d8a7c69636658fba4330d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10389.952999999994, "isoelectric_point": 9.12616062164307 }, "id": "LMJBGEANGM_924", "locus": "GCA013368015_00919" }, { "type": "cds", "sequence": "contig_1", "start": 531056, "stop": 531169, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCTTGTATATTCTAATGAACCTATTACAATTGTTGAACATGATGAAAAGCAAATTGCAACCATTAAAAATACTGTTCCTACTGGTTTTTTATTTTTATATGCAATGTAA", "aa": "MFLVYSNEPITIVEHDEKQIATIKNTVPTGFLFLYAM", "aa_hexdigest": "6e32a3089df65f17df82d5b5fbaeff0a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4274.9494, "isoelectric_point": 4.830996131896971 }, "id": "LMJBGEANGM_925", "locus": "GCA013368015_00920" }, { "type": "cds", "sequence": "contig_1", "start": 531274, "stop": 531465, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTATTGCTAAGTATCTAGCCAACTTATTTAAAACTAATGATTTAAAAATGGTATTTGATAATGCCAAATTAATTTTTGAAAAATCTAATTCTTTATCACTTGCTAATATTCCTAGTCTATTAACAGGTATTGATTTAGTAGGCGGTCAAAATACTAAAATTTATGGAATATATAATGGCCAAAATTGA", "aa": "MAIAKYLANLFKTNDLKMVFDNAKLIFEKSNSLSLANIPSLLTGIDLVGGQNTKIYGIYNGQN", "aa_hexdigest": "336621eee8bdff0d11fff1642d66237c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6908.945600000001, "isoelectric_point": 9.100760078430177 }, "id": "LMJBGEANGM_926", "locus": "GCA013368015_00921" }, { "type": "cds", "sequence": "contig_1", "start": 531790, "stop": 531969, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTAAGTGCATTTTTATATAATATTGCAATTACAGTATTTTTAAAAAAAGCAGCAACAATAGCTTCAGGAACTTCATCTTTATCTCAAATAATTACTTTTACAGCGCCTGCAACCGCACAATTTTATGGTTTATTTTATGTATTAGTTAACTTACCATTAATGTTTATTTATTGA", "aa": "MLVSAFLYNIAITVFLKKAATIASGTSSLSQIITFTAPATAQFYGLFYVLVNLPLMFIY", "aa_hexdigest": "1bf202222e35b1ddb3f6d176f53dd0d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6456.647599999998, "isoelectric_point": 9.303577613830566 }, "id": "LMJBGEANGM_927", "locus": "GCA013368015_00922" }, { "type": "cds", "sequence": "contig_1", "start": 531997, "stop": 532125, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTAGAAGATAAAAAAATAACTAAAATCAATAAAATCATTCTTACCAAAGAGCAAAAACACCAACGGTATTTAGAAAAAATGAAAATTCTAAATCCACAAAAAATAAATTTAATAAATGTCTGA", "aa": "MSLEDKKITKINKIILTKEQKHQRYLEKMKILNPQKINLINV", "aa_hexdigest": "1873dbb81ad7f096745ecd14bcf5105a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5077.1061, "isoelectric_point": 9.998482322692869 }, "id": "LMJBGEANGM_928", "locus": "GCA013368015_00923" }, { "type": "cds", "sequence": "contig_1", "start": 532165, "stop": 532698, "strand": "-", "frame": 3, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "nt": "ATGTATTTAAATGATAAACCTACATCAACAATTTCGGATATAACAACCATTGCAAAACGATTTGCTAGAAACAATAAAGTTGATCTAGTTGTAGTTGATTATCTACAACTAATGTCAGATTCTTCAAAAGCAAGTATGGAAAATCGTCAATTGGAAGTAAGCAAGATTTCGAGATCCTTAAAGCAACTTGCTAGAGAATTAGAATGTCCTGTTTTAGCATTAAGCCAGTTAAGTAGAAATGTTGAAAAAAGGGAAGATAAAACTCCTATTTTGTCAGATTTAAGAGAATCAGGAACAATTGAACAAGATGCCGATATGGTTATTTTTTTACATAGAAAAGATTATTATAATAAGCACAACATTAACGATGATAACGATAAAGTAGAAGCCATTGACAAAAGTGAAATTGTTAACGGCAGTTCCTCTTTAACCAATGTCATTGTAGCAAAAAATAGACATGGGGCTATTGGAATTGTACAACTATTATTCCAACCAGAATGCAACAGGTTTATTTATGAAAAAAAGAAAAATTAA", "aa": "MYLNDKPTSTISDITTIAKRFARNNKVDLVVVDYLQLMSDSSKASMENRQLEVSKISRSLKQLARELECPVLALSQLSRNVEKREDKTPILSDLRESGTIEQDADMVIFLHRKDYYNKHNINDDNDKVEAIDKSEIVNGSSSLTNVIVAKNRHGAIGIVQLLFQPECNRFIYEKKKN", "aa_hexdigest": "3a7b30a67c37dd8b920ce2b38560bea1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P211", "query_cov": 0.9322033898305084, "subject_cov": 0.9116022099447514, "identity": 0.6970000000000001, "score": 216.0, "evalue": 4.76e-69, "valid": false, "product": "Replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A449A6S1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A449A6S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "LMJBGEANGM_929", "locus": "GCA013368015_00924" }, { "type": "cds", "sequence": "contig_1", "start": 532726, "stop": 533169, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03796.21" ], "nt": "ATGGAAAACAAGCCTTATGAAATTTCAGATTTATTATCCAATTTACAATTGAATTTAATCAACCTAGATATTTCAGAAATAAATACTGCTTATGAACAAGTGGGAAATAAAGCAGATGAACTATTTAATAGAATAATGAGCAAAGAAAAATCAGATATTGGCGTTGGTCTCTCTTTTGGATTTCCACCATTGGATGAATTAATTTTAGGCGCCAATCCCGGAGATTTGATAATTTTAGCGGCTCGTCCAGCTATGGGAAAAACTGCTTTTGCTTTAAATATAGCAAATAATGTTGCCAAAAACAATAAAACGGTGCTATTTTTTTCTCTTGAAATGTCAAATTCACAAATTGTTCAAAGAATGATGTCAATTGATTCCTTGGTTCCGATTAGTTTAATGAGAAAAAATGAAATCTCAACAGAAAACGCTAAAAATCTAGCCTGA", "aa": "MENKPYEISDLLSNLQLNLINLDISEINTAYEQVGNKADELFNRIMSKEKSDIGVGLSFGFPPLDELILGANPGDLIILAARPAMGKTAFALNIANNVAKNNKTVLFFSLEMSNSQIVQRMMSIDSLVPISLMRKNEISTENAKNLA", "aa_hexdigest": "5c5f2f98c12d853157e1609e02d6be83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03796.21", "name": "DnaB_C", "length": 92, "aa_cov": 0.6190476190476191, "hmm_cov": 1.0, "evalue": 8.323204529191374e-30, "score": 99.5001449584961, "start": 56, "stop": 146 } ], "seq_stats": { "molecular_weight": 16173.475199999992, "isoelectric_point": 4.827301597595214 }, "id": "LMJBGEANGM_930", "locus": "GCA013368015_00925" }, { "type": "cds", "sequence": "contig_1", "start": 533281, "stop": 533562, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00772.26" ], "nt": "ATGAACATTCAAGAAGAAAGAGAATTAGCAATTATTAATAATGAAACATCGTTATTAGGTTTATTAATTAGGGAACCAAATTGTTATTTAACAATTGCTGAAATTTTAAAACCTGAAATGTTTCATTTTAAAAATAATCAAATTCTTTTTAAATCTATTTCAGAAGTTCATATTGCCTCTAAACAATTCGATATAGCCAACTTATTGAGTTATTTAAATGACCAAAAATTAATTGATCACATTGGTTCGTTTGGCTATAATGGTAGTGAATATATTAGCTGA", "aa": "MNIQEERELAIINNETSLLGLLIREPNCYLTIAEILKPEMFHFKNNQILFKSISEVHIASKQFDIANLLSYLNDQKLIDHIGSFGYNGSEYIS", "aa_hexdigest": "de7f77af7d54a33655db8caec9ccc4c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00772.26", "name": "DnaB", "length": 75, "aa_cov": 0.8064516129032258, "hmm_cov": 1.0, "evalue": 9.354229998162987e-16, "score": 53.34480285644531, "start": 12, "stop": 86 } ], "seq_stats": { "molecular_weight": 10717.135599999989, "isoelectric_point": 5.0043550491333 }, "id": "LMJBGEANGM_931", "locus": "GCA013368015_00926" }, { "type": "cds", "sequence": "contig_1", "start": 533590, "stop": 534030, "strand": "-", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_049342443.1", "SO:0001217", "UniParc:UPI00066D1476", "UniRef:UniRef100_UPI00066D1476", "UniRef:UniRef50_A0A318U7T8", "UniRef:UniRef90_D1J8I0" ], "nt": "ATGAAAGTAATATTAATTAAAGAATACCAAAAACATAAAAAAAATGAAATCATTGAAGTTACAGACGGTTTTGCAAAAAACTTTTTAATTAAAAATGGTTATGCTCAACCAATTAACAAACAAACATTGGCAAATTTAGATAGAGTTAAATCAAATATTGCAGAAGATTTAGCAAATCAAATAGCCAAGGCTAATGAAATCAAACAATTAATTGAATCATTAGTTTTAAAATTCTATTTAAAATCTAATGGAAATATAGTGCACGGAAACGTTACAACAAAAGCAATTGAAAAAGAATTAATAAAAAACAACATTAAAATCCCAAGCCATGCATTTGAATCAGGGGTAACATATAATACTTTTGGGTTGCATGATGTAAAAATTAAATTACATGACCAAGTAACTGCAATACTAAAAATAAATATCATAGAAGAGAAATAA", "aa": "MKVILIKEYQKHKKNEIIEVTDGFAKNFLIKNGYAQPINKQTLANLDRVKSNIAEDLANQIAKANEIKQLIESLVLKFYLKSNGNIVHGNVTTKAIEKELIKNNIKIPSHAFESGVTYNTFGLHDVKIKLHDQVTAILKINIIEEK", "aa_hexdigest": "ff0e7924a59e13854189cca27fbf2506", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00066D1476", "ncbi_nrp_id": "WP_049342443.1", "uniref100_id": "UniRef100_UPI00066D1476", "db_xrefs": [ "SO:0001217", "UniParc:UPI00066D1476", "RefSeq:WP_049342443.1", "UniRef:UniRef100_UPI00066D1476" ] }, "ips": { "uniref100_id": "UniRef100_UPI00066D1476", "uniref90_id": "UniRef90_D1J8I0" }, "psc": { "uniref90_id": "UniRef90_D1J8I0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A318U7T8", "cog_id": "COG0359", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318U7T8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U7T8" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "LMJBGEANGM_932", "locus": "GCA013368015_00927" }, { "type": "cds", "sequence": "contig_1", "start": 534011, "stop": 534334, "strand": "-", "frame": 2, "gene": null, "product": "DHHA1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "nt": "TTGCAAGAAATTAAAAAAGGATATTTCTTGGCATACAAAGATATTGAAGCATCAACAGATATTATTTCAATAGCGTCAGATGAAATACTAAGAGTTAATGGTAGGAAAGCTGCATTTGTTGTTGCTAAATTACCAGGAACAAAAACATACAAAATGTCAGCAAGAGGAATTAATACAAATGTTCAAATCATAGCAGAAGCAGTTAACGGCGGTGGCCATTTTGGGGCAGCCGCTGCTGAAACAACTGAAAATTTGAATTTATTTGTTGATAATATAATACAAGCAATAGTGAGTGTGAAAGATAATGAAAGTAATATTAATTAA", "aa": "MQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN", "aa_hexdigest": "203427b7f42c4d179281510b7caf3d55", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P200", "query_cov": 1.0, "subject_cov": 1.009433962264151, "identity": 0.757, "score": 152.0, "evalue": 7.27e-46, "valid": false, "product": "Putative bifunctional signaling protein/50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A432F9M3" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "product": "DHHA1 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_933", "locus": "GCA013368015_00928" }, { "type": "cds", "sequence": "contig_1", "start": 534416, "stop": 535579, "strand": "-", "frame": 2, "gene": "gdpP", "product": "GGDEF domain-containing protein", "db_xrefs": [ "COG:COG3887", "COG:T", "EC:3.1.4.59", "KEGG:K22927", "SO:0001217", "UniRef:UniRef50_A0A448ZXC6", "UniRef:UniRef90_D1J8I1" ], "nt": "ATGTATGAAATAGATGGATGCAATTACGAAGTTCATATATCATTTGAAAAAAATCTAGTTATTTTAAGAGATGTTACTGCTCAACAAAATTTATTAGATGACTATAAAAAACAAAGAATTGTGTTTGGGGAATTGGACATTGACAATATTAATTTATATCAAACATTTTTATCCCAAGAAGAAATTTTTAAAATTTATGCTTCTGTAGTAAATTTATTAGATGAAATATCTAAAAAATACAATGTTATTTATAGACAATACGAAAACGGAAGATTCTTTTTAATAACAAATAAAGAAACATTAGAAGAACTAGAACGAGATAGTTTTAATGTTATAACTTCGATAAAAAACAAAATAATTGTTAAAGATGTTTTAATATCAATTTCTGGTGGATTTGGTTATGGTATTTTTAAATACGATACGTTAGTCAAATTAGCAAAAGATGCTCTAATGCAATCACAAATGCGGGGTGGCGATCAAATTACAGTTTTAACAAAAAATGAAAAAGCTAGACACTATGGCTCAAGCTCGGAAATTGATGTAAATCTATCAAGAACAAATGTAAACTATATAGCACAAAACTTATTAGAAAAGATAAAATCAAAAGATATTGAAAATGTTATAGCCTATGGCCATAAGAATGCAGACCTTGATGCTTTGGGTTCAACATATGCAATTTTGCAATTAGCATTAGCCTATAAAAAGAATGCTTATATTCAAAACAAGACATTTGATAGTACTACTCAAAGGGTTATTGATAAAATGTCCAAGGAAACTCAAGAATATTTTATTAGTCCTAAAGAAGCAGAAAGCATCAACAACGAAAAAACGTTGGTTGTAATTTGCGATACTTCAGATGATACTAGAATTGAAAATGCTAATGTCTTTAAAAACATAAAAAAAGAAAATATCATGATAATTGATCATCATAGAATAAATAAAAATACTGATGAAATAGCATATAGAGAAAATGTATATATTGATTCCTCGGCATCATCTGCTTCTGAAATATTAACAGAAATCATCACATTGTTACACGAAGAAGATAAAATTAATAATGATACAGCGCAATATTTATTAGATGGAATTTATTTAGACACTAATACATTTAAAAAACAAACAAGTGCTAAAACTTTTGCAGCAGCATCAATATTGCAAAAATGA", "aa": "MYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSIKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANVFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQK", "aa_hexdigest": "f4adaf444fe17d0256e249a995fc58a6", "start_type": "ATG", "rbs_motif": null, "genes": [ "gdpP" ], "truncated": "both", "pseudogene": { "start": 534113, "stop": 535879, "observations": { "mutation": [ 534418, 535603, 535684 ], "stop": [ 534418, 535603, 535684 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I1", "query_cov": 1.0, "subject_cov": 0.589041095890411, "identity": 0.997, "score": 741.0, "evalue": 2.36e-265, "gene_start": 1, "gene_end": 387, "reference_start": 136, "reference_end": 522, "reference_sequence": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKIIWASESLKIKFHNNLINKNIKDIFNIKQWTSDNQEFMYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSIKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANAFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQKWGAQNDKSVDTLKFDEKVFKIVQELLANLQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN" }, "paralog": false, "identity": 0.9931972789115646, "score": 1123.0, "evalue": 0.0, "description": "Internal stop codon at 534418, 535603, 535684. Nonsense mutation around 534418, 535603, 535684.", "psc": { "uniref90_id": "UniRef90_D1J8I1", "gene": "GdpP", "product": "GGDEF domain-containing protein", "ec_ids": [ "3.1.4.59" ], "uniref50_id": "UniRef50_A0A448ZXC6", "cog_id": "COG3887", "cog_category": "T", "kegg_orthology_id": "K22927", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I1", "EC:3.1.4.59", "UniRef:UniRef50_A0A448ZXC6", "COG:COG3887", "COG:T", "KEGG:K22927" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXC6" ], "product": "Probable manganese-dependent inorganic pyrophosphatase" } }, "id": "LMJBGEANGM_934", "locus": "GCA013368015_00929" }, { "type": "cds", "sequence": "contig_1", "start": 535682, "stop": 535984, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGATATTCAAAAAATTTATATAGCTTTACAAGTTATCCTATTATTTATTTTGCCAACAATATTATTCTTTATTGGCATTTTTTGTTTAAAGACTCAACAGTCAAAAATAGCGTTAATGGCAATTTACACTTTATTTTTAATCGGCCTTGCAATTTATTTAACTATTATTTTGCAACGTTATTTTAAAAACTCAATATTAGCTAAAAAATCATTGGAATTTTATTTACAAAGCGAATTCAATCAATTTGGTACGGGAATTTTGATATTTTTAAATAGTGGAAAAATTATATGA", "aa": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKII", "aa_hexdigest": "93913c5e64e6e5d77a593477eabc9f08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11558.992599999985, "isoelectric_point": 9.763881874084472 }, "id": "LMJBGEANGM_935", "locus": "GCA013368015_00930" }, { "type": "cds", "sequence": "contig_1", "start": 536038, "stop": 536322, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "SO:0001217", "UniRef:UniRef50_B3PN57", "UniRef:UniRef90_D1J8I2" ], "nt": "ATGATTGACTTTTATTTTAAAAACGATAAAAACAATAAAAAAGTGTTGCTATTAATTGATGCTAAAATTGGTTTTAGCGCTAGTGATTTAGAAATAATAGAATATCTTAACGATCTTAAAATAAAATTTGATATAGTTGTTACAAAAATCGACAAGGCAAATCAATCTCAACGTTATTGCGTTAAAAATCAAGCCTTATCTTTAAAAACAGCTGAAAATATTTTTTTAGTATCATCCGAAAAAAAACAAGGTATTGAAGAAATAATGCAATTTTATGACTTGTAG", "aa": "MIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTAENIFLVSSEKKQGIEEIMQFYDL", "aa_hexdigest": "7bbafeda14442b568ac563ccdcc705ea", "start_type": "ATG", "rbs_motif": null, "genes": [ "yihA" ], "truncated": "3-prime", "pseudogene": { "start": 536038, "stop": 536589, "observations": { "mutation": [ 536517, 536586 ], "stop": [ 536517, 536586 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I2", "query_cov": 1.0, "subject_cov": 0.5136612021857924, "identity": 0.9890000000000001, "score": 182.0, "evalue": 3.15e-57, "gene_start": 1, "gene_end": 94, "reference_start": 90, "reference_end": 183, "reference_sequence": "MWKFLKSCKSHDDFINSDNIQYVFWGRSNVGKSSLINSLASCNIAKTSSYPGRTQLINYFESNKNKIIIDLPGYGYAKISKKLQDKISFMIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTVENIFLVSSEKKQGIEEIMQFYDL" }, "paralog": false, "identity": 0.9836065573770492, "score": 353.0, "evalue": 8.43e-127, "description": "Internal stop codon at 536517, 536586. Nonsense mutation around 536517, 536586.", "psc": { "uniref90_id": "UniRef90_D1J8I2", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_B3PN57", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I2", "UniRef:UniRef50_B3PN57", "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN57" ], "product": "Probable GTP-binding protein EngB" } }, "id": "LMJBGEANGM_936", "locus": "GCA013368015_00931" }, { "type": "cds", "sequence": "contig_1", "start": 536589, "stop": 536975, "strand": "-", "frame": 1, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAATTTAGAGATAAATACACAGGACATTTTACTATTCCACATACTGAAGCTCAATTGAACTTTTCATTTAGTGGATTAAAAACACAAGTTATAAATCTTATAAATAACCAAATCAATAGAAAACAAGAGATCAATGTTGATCAATATGCAACAATGTTTGAAAATACAGTAGTCTTATATTTAAAAAATCATTTAGAAATTGCAATTAAAAAATATCATCCAAGAGTAATTGTTTTGGCAGGCGGAGTAAGTGCTAATAAAGCAATTAGAGAAATGTTTTTAAATGTCCATTCAAATGCCATAATTCCGGAAATGAAATATACAACTGATAATGCAATGATGATTGCTAGGTTAGCATATGAACTATTAAGGAGAGATAGATAA", "aa": "MEFRDKYTGHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR", "aa_hexdigest": "1a9bc545735fd0240b7e903a7a028b62", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 536589, "stop": 537275, "observations": { "mutation": [ 536978 ], "stop": [ 536978 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.4115755627009646, "identity": 0.992, "score": 249.0, "evalue": 5.36e-81, "gene_start": 1, "gene_end": 128, "reference_start": 184, "reference_end": 311, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9912280701754386, "score": 447.0, "evalue": 1.09e-161, "description": "Internal stop codon at 536978. Nonsense mutation around 536978.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "LMJBGEANGM_937", "locus": "GCA013368015_00932" }, { "type": "cds", "sequence": "contig_1", "start": 536976, "stop": 537449, "strand": "-", "frame": 1, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAAACAATTAGCCAAATAGACATTCATAGAAAATATGGAGGCACAGTTCCAGAATTAGCATCAAGATTGCATGTAAAAAACATAGGTATTTTAATTGAAAAATTAAAAAAAATAATTGATATTAAAAAGATTGACTATGTAGCATATACAAAAGAACCGGGATTAGTTGGCTCTTTACATGTTGGATATGTTGTTGCTAATGCAATAGCAATGTTACTTGAAAAACCTATATTGCCTTTGAATCATTTAGAAGGGCATTTTTTCTCTGCTTTTATAGGCAAAGAAGTTGAATATCCTGCTTTAGGTTTAATTGTTTCAGGTGGGCATTCACAATTAATGTTATATAAATCAAAAAATGAATACGAAGTTATAGGCCAAACTCAAGATGATGCCGTCGGAGAAGTATTTGATAAAATAGCAAGAAAATTAAATTTGGGTTTTCCTGGAGGACCTATTATTGATAAAAAATGA", "aa": "METISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKK", "aa_hexdigest": "2d6f9daa68d37cb12cda0f81d33e3a2a", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "both", "pseudogene": { "start": 536673, "stop": 537524, "observations": { "mutation": [ 536978, 537452 ], "stop": [ 536978, 537452 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.5048231511254019, "identity": 1.0, "score": 315.0, "evalue": 1.89e-106, "gene_start": 1, "gene_end": 157, "reference_start": 26, "reference_end": 182, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9893992932862191, "score": 551.0, "evalue": 3.82e-202, "description": "Internal stop codon at 536978, 537452. Nonsense mutation around 536978, 537452.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "LMJBGEANGM_938", "locus": "GCA013368015_00933" }, { "type": "cds", "sequence": "contig_1", "start": 537518, "stop": 538072, "strand": "-", "frame": 2, "gene": "tsaB", "product": "Gcp-like domain-containing protein", "db_xrefs": [ "COG:COG1214", "COG:J", "RefSeq:WP_012855670.1", "SO:0001217", "UniParc:UPI0001BC23D2", "UniRef:UniRef100_D1J8I4", "UniRef:UniRef50_D1J8I4", "UniRef:UniRef90_D1J8I4" ], "nt": "GTGAAATTATTCATTGAAACTTCATTAAGCGATTTTTATATAAGCTTAATTGATGAAAATTATTGCGAGATTGATTGTATTTTTATTGCTAACTTAGTTAAAAAAACCGATGCATTTTTTGAAAATTTAAATTATTTATTAAATAAAAATAATTTTAAATTAAGTTCAATTTCAGAAATTTATACAACGTTAGGACCTGGGAGTTTTTCAGGTGCAAGAATTGGCTTAGTTTTTGCCAAAACAGTAGCACAATTATCAAATATCAAATTATTTGTAGCCCCAACATATTCTTTATTTTATACACAAAAAAAATTGCAACATGAAGATTGCAATTTAATAAATATAAAAGCCAATAAATACAATGTTTATTCAATAATTGTTGATCCATTTGAATGTCATATGCAAGAAAACTTAGGCAATTTTGATTTATTCAATAAAGAGCTTTTTTCAAAAAATATAGAGCAATATTTAAAAGAATTTAAACAAGTAAATGATATTGAAAATTGCGAATTAATTTATTTGCATGATCCACAAATAGGAGAGATAAAATGTTAA", "aa": "MKLFIETSLSDFYISLIDENYCEIDCIFIANLVKKTDAFFENLNYLLNKNNFKLSSISEIYTTLGPGSFSGARIGLVFAKTVAQLSNIKLFVAPTYSLFYTQKKLQHEDCNLINIKANKYNVYSIIVDPFECHMQENLGNFDLFNKELFSKNIEQYLKEFKQVNDIENCELIYLHDPQIGEIKC", "aa_hexdigest": "f5bc757e9aebf1457d73e00cc13b0baa", "start_type": "GTG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC23D2", "ncbi_nrp_id": "WP_012855670.1", "uniref100_id": "UniRef100_D1J8I4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC23D2", "RefSeq:WP_012855670.1", "UniRef:UniRef100_D1J8I4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8I4", "uniref90_id": "UniRef90_D1J8I4" }, "psc": { "uniref90_id": "UniRef90_D1J8I4", "gene": "TsaB", "product": "Gcp-like domain-containing protein", "uniref50_id": "UniRef50_D1J8I4", "cog_id": "COG1214", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8I4" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "LMJBGEANGM_939", "locus": "GCA013368015_00934" }, { "type": "cds", "sequence": "contig_1", "start": 538174, "stop": 538470, "strand": "-", "frame": 3, "gene": "tsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "db_xrefs": [ "COG:COG0802", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3G8LG14", "UniRef:UniRef90_D1J8I5" ], "nt": "ATGTCAAATCTAAAATTTATTTATAAAAATAACGAACCATTAGATAAACTAGTTGACTATATTTTGTCATTTAAAAATTTAGAAGCCTTGCTTTTAATAGGCGAACTCGGAGCAGGCAAAACAACTTTAACAAGTCAAATAGCAAAAAAATTAAACGAACCAAAGACTGTAATATCACCCACATTCAATACGATTTTAGTGTATGATAAATTTGTACATATTGATGCTTATAAATTAACTACAAATTTATTTGCTTATGAAGATTATTTTGAAGATAAATTAGCAATTATTGAATGA", "aa": "MSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIE", "aa_hexdigest": "d4dfdacce5f9d287a456a83f5ae9929e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaE" ], "truncated": "5-prime", "pseudogene": { "start": 538072, "stop": 538476, "observations": { "mutation": [ 538176 ], "stop": [ 538176 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I5", "query_cov": 1.0, "subject_cov": 0.7313432835820896, "identity": 1.0, "score": 191.0, "evalue": 3.45e-61, "gene_start": 1, "gene_end": 98, "reference_start": 3, "reference_end": 100, "reference_sequence": "EIMSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIEWAENVTLPKFKHYIKIKVYFDNEQNHVFEVMEEQ" }, "paralog": false, "identity": 0.9925373134328358, "score": 265.0, "evalue": 1.19e-92, "description": "Internal stop codon at 538176. Nonsense mutation around 538176.", "psc": { "uniref90_id": "UniRef90_D1J8I5", "gene": "TsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "uniref50_id": "UniRef50_A0A3G8LG14", "cog_id": "COG0802", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I5", "UniRef:UniRef50_A0A3G8LG14", "COG:COG0802", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LG14" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" } }, "id": "LMJBGEANGM_940", "locus": "GCA013368015_00935" }, { "type": "cds", "sequence": "contig_1", "start": 538653, "stop": 539342, "strand": "-", "frame": 1, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGCAAGAAGAAGAAAGAAAGAAAGCAATTACAGAAAATGAATTATTTGCTGATTTTGGTTTCAAAAGCGATGAAGATGCTATCGCTCATGGTGTTAATGTTGGCGATAGAGTTTACATGAGTGGAGAAACAATACTATTTAATGATCAAAATTTAATTGGCGGAAAAGCAATGGACAACCGTGCTGGTGTAACCGTGTTAGAATTTGTTGCTAAAGCTGTTGCAAAATTAGATCTTGACATTGATCTATATTTAGTAGGTACAGTTCAAGAAGAAGTTGGAACAAGAGGAGCTAAAACAGTTGCGTCTATGATTGATCCAGACATTGCAATAGCAGTTGATACTTCTGCATCTCACGATACACCTAATACAATTCCTGGGAATGTTTCTTTATATAAAGGAGCTGCTTTAAGATTAAAAGATAGAGGAACAATGATGGATCCAAAATTGGTACAATTTTTTGTTGATTTATCTAAGAAATATAATATTCCAACATTTAAATATGTAGCCATGGGTGGTGGCACTGATGCCTTTGAATTGCAATTTTCAAAAGGTGGAGCAGCCACATTAACAATTTCATTACCTCAAAGATATTTGCATTCACCAATCGGAGTATGTTGCATAAGTGATTTACTAGCAGCTGTTGATTTGTTAACAAAATTTTTAACTGATTTTAAACAATCTGATTGA", "aa": "MQEEERKKAITENELFADFGFKSDEDAIAHGVNVGDRVYMSGETILFNDQNLIGGKAMDNRAGVTVLEFVAKAVAKLDLDIDLYLVGTVQEEVGTRGAKTVASMIDPDIAIAVDTSASHDTPNTIPGNVSLYKGAALRLKDRGTMMDPKLVQFFVDLSKKYNIPTFKYVAMGGGTDAFELQFSKGGAATLTISLPQRYLHSPIGVCCISDLLAAVDLLTKFLTDFKQSD", "aa_hexdigest": "2650e29473978c1d547374998b132b41", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8733624454148472, "subject_cov": 0.9478672985781991, "identity": 0.735, "score": 296.0, "evalue": 1.58e-99, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "LMJBGEANGM_941", "locus": "GCA013368015_00936" }, { "type": "cds", "sequence": "contig_1", "start": 539433, "stop": 539717, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAGAAAATCAATTCAAAGAAAGATTAATAAAATATATGGAAATTGAAGCAATGTCTCGTTACGAAGAACCAGTTGTTAATGAATTAAAAAAAGAATTAGCTAATGAAGGATTTGAATTTAGCAGAGATAATTTTGGCTGTTTAATTATGCACAAGCCATCTAAAAATCCAAATGCTCCTAAGGTTTTATTAGCAGCACACATGGATGAAGTTGGATATGTAGTAAGAAATTTTGATAAAAAAGGACAATTATATTTATCAACAGTTGGTGGTGTATGA", "aa": "MTRENQFKERLIKYMEIEAMSRYEEPVVNELKKELANEGFEFSRDNFGCLIMHKPSKNPNAPKVLLAAHMDEVGYVVRNFDKKGQLYLSTVGGV", "aa_hexdigest": "3fc428d1e9f98d963fffb68baebbdbb2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10869.407299999992, "isoelectric_point": 6.733567619323731 }, "id": "LMJBGEANGM_942", "locus": "GCA013368015_00937" }, { "type": "rRNA", "sequence": "contig_1", "start": 539853, "stop": 542748, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9900854700854701, "score": 2764.5, "evalue": 4.9e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTACAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGACGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTTATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "LMJBGEANGM_39", "locus": "GCA013368015_00938" }, { "type": "rRNA", "sequence": "contig_1", "start": 542931, "stop": 544448, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1513.0, "evalue": 2.6e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGCAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "LMJBGEANGM_35", "locus": "GCA013368015_00939" }, { "type": "cds", "sequence": "contig_1", "start": 544725, "stop": 544952, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAAGAAGCAATGTTGCTAGCTAAATTTAAAGCAGGGGACATTGAAACTGCTTTTAAAGAAGGGGCTTTAGTAAAAATTTCTAGTGGAATATTCAAAGATGAAGTTGGCGAAATAATTAAAAATAATGATATTACCCAAAAAGCCTTTGTTAATATTGAACAATTTGGTAGAAAAGTTCCAACTGAATTTGATTATGCAGATCTAGAAATAGTAGGTTAA", "aa": "MIDKEAMLLAKFKAGDIETAFKEGALVKISSGIFKDEVGEIIKNNDITQKAFVNIEQFGRKVPTEFDYADLEIVG", "aa_hexdigest": "6cd0e41740e267b0c0a651441ba51b2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8339.500699999999, "isoelectric_point": 4.7058935165405265 }, "id": "LMJBGEANGM_943", "locus": "GCA013368015_00940" }, { "type": "cds", "sequence": "contig_1", "start": 545049, "stop": 545279, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02357.24" ], "nt": "ATGATTTCAACAGTTTCAGGCAAAGAAGAAAATGTTATAGAATCATTAAAGAATAAAATTGCTGCTCAAAGAATGGATGAATACTTTAAAGATATAAGGATGTTTAAAGAACCCCATTTATCAAATAAAGAATTAGAAAAGAAGCAAAGAAACGAAGAATTTACTGTCAAATTTGTAAATATGTACAAAGGTTATATTTTCTTAAATATGGCAATGACTGATGATACATGA", "aa": "MISTVSGKEENVIESLKNKIAAQRMDEYFKDIRMFKEPHLSNKELEKKQRNEEFTVKFVNMYKGYIFLNMAMTDDT", "aa_hexdigest": "83445adab5e4fdbba14afdcaba235a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02357.24", "name": "NusG", "length": 74, "aa_cov": 0.9736842105263158, "hmm_cov": 0.7432432432432432, "evalue": 2.464978870958742e-08, "score": 29.945831298828125, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 9073.367599999994, "isoelectric_point": 6.579306602478028 }, "id": "LMJBGEANGM_944", "locus": "GCA013368015_00941" }, { "type": "cds", "sequence": "contig_1", "start": 546007, "stop": 546444, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241194BE", "UniRef:UniRef90_UPI00241194BE" ], "nt": "TTGAACAATGATGAATGTAAGGAACTAGAAAAACAAATTGGAATTAATTTGCATGAAGTTTGTAGTTTATACAAGGCATGTAAAATATTAAAAACCAAAGTAGACGATATTGTTTTTTTGCATATGAACAAGCATGCTTTTTGGCATTTAAAATGCACAATTCTTATTAAAAAGAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAGCCATCTTTAAAAAAGCGGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATAATAAATAAATACAAAGTTAGTTTAAATGATTTGTCAAATATAATGGCAGGTGAACATGAACATTTTCCTCAACACTTTATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAACGATGAATATCTAAAATGA", "aa": "MNNDECKELEKQIGINLHEVCSLYKACKILKTKVDDIVFLHMNKHAFWHLKCTILIKKKYKEIYFKRLEIFNLLEAIFKKAAEIDKKSGNIQKIINKYKVSLNDLSNIMAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLK", "aa_hexdigest": "51125753edbc717e36a70e4693f5102d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 545815, "stop": 546663, "observations": { "mutation": [ 545824, 545953, 546442 ], "stop": [ 545824, 545953, 546442 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241194BE", "query_cov": 1.0, "subject_cov": 0.5141843971631206, "identity": 0.993, "score": 293.0, "evalue": 1.44e-98, "gene_start": 1, "gene_end": 145, "reference_start": 65, "reference_end": 209, "reference_sequence": "MSKWAILFLVILIMLIAMYLTFIFYYFIKVYPQVLKSKLDNFINKIWTFINKAKNSYDKICQLSLNNDECKELEKQIGINLHEVCSLYKACKILKTKVDDIVFLHMNKHAFWHLKCTILIKKKYKEIYFKRLEIFNLLEAIFKKAAEIDKKSGNIQKIINKYKVSLNDLSNIMAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLKWNEKILKQNLLEIKNLIIEYATFYFKFKNNYKNLNYIAEKYKKNQQLIKELNNIKLEQLIKEMLEKNLNLIMR" }, "paralog": false, "identity": 0.9893617021276596, "score": 553.0, "evalue": 2.01e-203, "description": "Internal stop codon at 545824, 545953, 546442. Nonsense mutation around 545824, 545953, 546442.", "psc": { "uniref90_id": "UniRef90_UPI00241194BE", "uniref50_id": "UniRef50_UPI00241194BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241194BE", "UniRef:UniRef50_UPI00241194BE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241194BE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241194BE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17253.125999999997, "isoelectric_point": 9.132994270324708 }, "id": "LMJBGEANGM_945", "locus": "GCA013368015_00942" }, { "type": "cds", "sequence": "contig_1", "start": 546684, "stop": 546980, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAACAAATGTCAAAATTCAAAAGACATTTCAAATATCGAACAAATTATTAATAAACTATTGGCTTTGATAAATACAACTAGTATAAAAATCTATAACTATATTAAAACTTTCAACTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGACGTTCAAAATAAAAAAATTTCTGATTTTTTTGCAAGTGAAACTAAAAAGAATTTATTAAAAAGTGAATACCTTAATACTTATAAGCCAATATTGAATAGTTTTATAGATATTAAGAATAATAATTGA", "aa": "MINKCQNSKDISNIEQIINKLLALINTTSIKIYNYIKTFNFHLKIQNNIKKIIEKIDVQNKKISDFFASETKKNLLKSEYLNTYKPILNSFIDIKNNN", "aa_hexdigest": "b6797e6458d813db1cd3b38e64c12c33", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11539.336299999984, "isoelectric_point": 9.650997734069826 }, "id": "LMJBGEANGM_946", "locus": "GCA013368015_00943" }, { "type": "cds", "sequence": "contig_1", "start": 546981, "stop": 547469, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410F5F0", "UniRef:UniRef90_UPI002410F5F0" ], "nt": "ATGGATATAGTTGAATTTATAGAGTTGAAGAATTGGTTAGAATCTTTATTGAATTTGCTAAGGATTCAAAATAAAATAATAAATAAAATAGATGAACAAGAATATCGAAACAATCAGATTGAATTCTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAATATTAAATATTAGTTTAAGTTCAGAAGACATTGAAAACATAAATAACTTATTTGCTTTTATTTATAAATTCAAAAATAATAATTTTTCTGCAAACGAATTGAATGACTTTTTAAGTTATTTATCTAATGAAATAAATTTTTTAAATTGCAAAATTCAATATTTTTTAATAATTAAAAATGTAGAATTTTCTTTAAGAAAAGATGTAAGTGAAAATTCAAATGCCCAAAATTTATTTAATGAGTTGCAAAAACTTAAAGGTTACAATGATGATAAAAAAGCAATTGATTTAATATATGATTATATAGAAGAACAATACAACTAA", "aa": "MDIVEFIELKNWLESLLNLLRIQNKIINKIDEQEYRNNQIEFYIENFKRLTANIKILNISLSSEDIENINNLFAFIYKFKNNNFSANELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN", "aa_hexdigest": "dbd22134c0cdebad4ffd0e0404250467", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 546684, "stop": 547469, "observations": { "mutation": [ 546978 ], "stop": [ 546978 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410F5F0", "query_cov": 1.0, "subject_cov": 0.6206896551724138, "identity": 1.0, "score": 309.0, "evalue": 5.8e-105, "gene_start": 1, "gene_end": 162, "reference_start": 100, "reference_end": 261, "reference_sequence": "MINKCQNSKDISNIEQIINKLLALINTTSIKIYNYIKTFNFHLKIQNNIKKIIEKIDVQNKKISDFFASETKKNLLKSEYLNTYKPILNSFIDIKNNNWMDIVEFIELKNWLESLLNLLRIQNKIINKIDEQEYRNNQIEFYIENFKRLTANIKILNISLSSEDIENINNLFAFIYKFKNNNFSANELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN" }, "paralog": false, "identity": 0.9923371647509579, "score": 511.0, "evalue": 3.58e-187, "description": "Internal stop codon at 546978. Nonsense mutation around 546978.", "psc": { "uniref90_id": "UniRef90_UPI002410F5F0", "uniref50_id": "UniRef50_UPI002410F5F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410F5F0", "UniRef:UniRef50_UPI002410F5F0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410F5F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410F5F0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19492.820999999996, "isoelectric_point": 4.740451622009277 }, "id": "LMJBGEANGM_947", "locus": "GCA013368015_00944" }, { "type": "cds", "sequence": "contig_1", "start": 547480, "stop": 547578, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22025.1" ], "nt": "ATGGACTACAATAAAATTTTAATTAGATATGGTGAATTGACTCTTAAAGGCGAAAATAAAAAAGATTTTATTAGGCAATTGCATAACAACCTATCTTGA", "aa": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLS", "aa_hexdigest": "fadac77c9c73137a76016e2ce1732acf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22025.1", "name": "ThiI_fer", "length": 26, "aa_cov": 0.8125, "hmm_cov": 1.0, "evalue": 5.1055039981668634e-12, "score": 41.67063522338867, "start": 6, "stop": 31 } ], "seq_stats": { "molecular_weight": 3865.4184000000005, "isoelectric_point": 9.301772499084471 }, "id": "LMJBGEANGM_948", "locus": "GCA013368015_00945" }, { "type": "cds", "sequence": "contig_1", "start": 547807, "stop": 548628, "strand": "+", "frame": 1, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "SO:0001217", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_D1J8J1" ], "nt": "ATGACTTCAAGTGAACTAAATATGTATTTTGGAGGGGTTGTTTTAAAACAAATGCCAGACAAGGAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGATGGCTTAGGTGGTATGCCGGTATTGAGTTCTGGAAAATGCATACATCTAATTAGTGGAGGCATTGACTCGCCTGTAGCAGCGAACTTATTACAAAAAAGAGGGCTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACAGATGAAAAAACAATTGCAAAAATAGATGAAATTGTCAATATATTAAAAAAATACCAAGGTAATGCTATGTTATATCAAATTAACTATAGCAAAGTAATGGAATATATTGGCCTTATAAGTAATCAAAAATACAAAATAACTTTAATGAGAAGATCATTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGTGAAAATTTAGCGCAAGTTGCCTCTCAAACCCTTGAATCTATTTATACTATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCCTTAATTTCTTTTGACAAAAATGAAACTATTGCTATTGCAAAAAAAATTAACACATTAGATGTTTCAATAGTAAGGGCATGTGAAACATGTGAATTATTTGCTCCTAAAAATCCTGTAACCAAGCCTAATAGGCAAGAAGCTCATAAACTCGAAGAAGAAGAATTACCTTTAATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAA", "aa": "MTSSELNMYFGGVVLKQMPDKEVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAHKLEEEELPLIKEFEEECIKNAIIKKYSI", "aa_hexdigest": "3d53dd9491921d0ad95ee415f5af8fee", "start_type": "ATG", "rbs_motif": null, "genes": [ "thiI" ], "truncated": "5-prime", "pseudogene": { "start": 547507, "stop": 548628, "observations": { "mutation": [ 547576 ], "stop": [ 547576 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J1", "query_cov": 1.0, "subject_cov": 0.7146596858638743, "identity": 0.982, "score": 528.0, "evalue": 1.77e-187, "gene_start": 1, "gene_end": 273, "reference_start": 110, "reference_end": 382, "reference_sequence": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLSWYIEKSEIKTEYDRAFVDYSQKNLDCLKYIFGISSYSPVYETETSLINIENIVKEIISKKQFDSFAINARRHNKNFSMTSSELNMYFGGIVLKQMPDKQVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAYKLEEEELPLIKEFENECIENAIIKKYSI" }, "paralog": false, "identity": 0.9758713136729222, "score": 722.0, "evalue": 1.21e-266, "description": "Internal stop codon at 547576. Nonsense mutation around 547576.", "psc": { "uniref90_id": "UniRef90_D1J8J1", "gene": "ThiI", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2", "cog_id": "COG0301", "cog_category": "HJ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J1", "UniRef:UniRef50_B3PLZ2", "COG:COG0301", "COG:HJ" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" } }, "id": "LMJBGEANGM_949", "locus": "GCA013368015_00946" }, { "type": "cds", "sequence": "contig_1", "start": 548681, "stop": 548794, "strand": "-", "frame": 2, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "nt": "ATGCCTCCAACAGGCGGTTCTGGTATAGGAATTGATCGTTTAATTATGCTATTAACTCAAAATGATTCAATTAGAGATGTAGTTTTATTCCCTCAATTAAAAGATTTAAAATAA", "aa": "MPPTGGSGIGIDRLIMLLTQNDSIRDVVLFPQLKDLK", "aa_hexdigest": "d9b52268f805181a0d249c6481f62c51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920J7V4", "query_cov": 0.8378378378378378, "subject_cov": 0.8611111111111112, "identity": 0.677, "score": 46.2, "evalue": 5.74e-06, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920J7V4" }, "pscc": { "uniref50_id": "UniRef50_A0A920J7V4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_950", "locus": "GCA013368015_00947" }, { "type": "cds", "sequence": "contig_1", "start": 548822, "stop": 549319, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGAAAGAACTGAAGGCGTTTTCAAACACTTAATAAATAAATTCAATATTAAAGAAGTTAAATTTAATGGCTTTGATATAACATTTAATTTTCCATTTAAGAGAGTTAATATGATTGATGCTATATCTGAAAAGATTAATGTTGACATTAGAAAATTAAATGAAGAACAAATGTTTGAATTAGCTAAAAAACATGGTATTAAAGTTGAAAAATTCTTTAGATATGGTCACATTATCAATGAATTATTTGAAAAATATGTTGAAGAAACATTAATAGAACCAACATTTGTATATGGTCACCCAATTGAAATTTCTCCTCTTGCAGCAATTGACCCTGACGATAAAAGATTTACACTACGTGCTGAACTATTTATTGGAACAAAAGAATTTGCAAATATGTTTACTGAATTAAATGATCCAATTGACCAATTGCAACGTTTTGAAAACCAATTGTTGGAAAAAGAAAATGGTAATGAAGAAGCAAATGAAATTGATTGA", "aa": "MERTEGVFKHLINKFNIKEVKFNGFDITFNFPFKRVNMIDAISEKINVDIRKLNEEQMFELAKKHGIKVEKFFRYGHIINELFEKYVEETLIEPTFVYGHPIEISPLAAIDPDDKRFTLRAELFIGTKEFANMFTELNDPIDQLQRFENQLLEKENGNEEANEID", "aa_hexdigest": "63d8fd55075fd618c782f9a6c8b2305e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0027D2801B", "query_cov": 0.9030303030303031, "subject_cov": 0.8097826086956522, "identity": 0.53, "score": 147.0, "evalue": 4.83e-42, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0AA38HFP6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 165, "aa_cov": 0.9636363636363636, "hmm_cov": 0.806060606060606, "evalue": 8.051263218043948e-15, "score": 50.169349670410156, "start": 1, "stop": 159 } ], "seq_stats": { "molecular_weight": 19551.041799999995, "isoelectric_point": 4.986791801452636 }, "id": "LMJBGEANGM_951", "locus": "GCA013368015_00948" }, { "type": "cds", "sequence": "contig_1", "start": 549326, "stop": 550147, "strand": "-", "frame": 2, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "nt": "ATGGAAAGAAAACTTAGTGAACAAGAAGAAATTAGACGTAATAAAATTGCTGAATTAGAAAAAAACAATTTACAAGCATTTCATAAAACTATAAAACCAACAGCAAATTCAAGAGAATTAAATGAAAAATATAAAAATTTAAGCCGTGAAGATCTTGACAAACAAAATCTAGAATTTGTATTTAATGGAAGAATTATGACTATTCGTGGCCCATTTATTGTTTTACAAGATAATTGCGGACAAATGCAAATTTATGCAAATAAAAAAACATTGAATGAGTCTTCTGCAAAAACCTTTTCTTTAATGGATTTTGGTGATATTTTAAGTGTAAGAGGAACATTGTCAAAAACTAATACTGATGCTTTAGTTATAAAGGCTCAAGACATAGTTCTATTGACAAAATCATTGAAACCGTTGCCTGATAAATTTCATGGTTTAGTTGATCCTGAAGAAAGACGTCGTCAACGCTATGTAGATTTAATAATGAATGAAAAAACAAGAAATACATTTGTTATGAGAACCCAAATTATCAAAGGCATTAGAGAATATTTTGATAGTTTGAACTATATTGAAGTTGATACACCAATTTTAAATCCAATATTAGGTGGTGCTGCTGCTAAACCTTTTATTACTTTCTATAATGCACTTAATAGAAACTTTTATTTAAGAATTGCAACTGAATTGCCATTAAAAAAATTGATTGTTGGTGGAATGGATAGAGTTTATGAAATCGGCAGAATATTTAGAAATGAAGGCGTAGATACAACTCATAATCCAGAATTTACCTCAATTGAATTTTATGAAGCATATTCAGATTTATGA", "aa": "MERKLSEQEEIRRNKIAELEKNNLQAFHKTIKPTANSRELNEKYKNLSREDLDKQNLEFVFNGRIMTIRGPFIVLQDNCGQMQIYANKKTLNESSAKTFSLMDFGDILSVRGTLSKTNTDALVIKAQDIVLLTKSLKPLPDKFHGLVDPEERRRQRYVDLIMNEKTRNTFVMRTQIIKGIREYFDSLNYIEVDTPILNPILGGAAAKPFITFYNALNRNFYLRIATELPLKKLIVGGMDRVYEIGRIFRNEGVDTTHNPEFTSIEFYEAYSDL", "aa_hexdigest": "33057ce7f832ae5d7f8abea7172d7d87", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EED3E5F", "query_cov": 0.9926739926739927, "subject_cov": 0.8885245901639345, "identity": 0.539, "score": 288.0, "evalue": 3.03e-94, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_UPI001EED3E5F", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_UPI001EED3E5F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "LMJBGEANGM_952", "locus": "GCA013368015_00949" }, { "type": "tRNA", "sequence": "contig_1", "start": 550239, "stop": 550313, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 68.0, "nt": "GGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 550268, 550270 ], "id": "LMJBGEANGM_23", "locus": "GCA013368015_00950" }, { "type": "cds", "sequence": "contig_1", "start": 550586, "stop": 550768, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGACATCCAATCATTCTTTGGCAATGAACAAGGAATGAATTTTGTTGAACTTGATGAAAGTAAAAGAAAAGCTCACATATTTTATGCCAAAATTTTCTTTATAAGTATCGCAGTTTTAATTGTATTGCCCTTTATAATATATATTATTTTAAAGAAGACAGTATTGAAAGGAAAGTAG", "aa": "MVDIQSFFGNEQGMNFVELDESKRKAHIFYAKIFFISIAVLIVLPFIIYIILKKTVLKGK", "aa_hexdigest": "5a4badaa5c8b1817fbaff71a052f1d35", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6966.339399999997, "isoelectric_point": 9.45623874664307 }, "id": "LMJBGEANGM_953", "locus": "GCA013368015_00951" }, { "type": "cds", "sequence": "contig_1", "start": 551076, "stop": 551225, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCTTATGTATTCCTGAAAGTTCAATCAATTCAATAGTAAATCTAGTTAATGAAATTACTATAAATATTGCTGGAATTATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTGAATAAATTTTGTCAAAAGTAA", "aa": "MLLCIPESSINSIVNLVNEITINIAGIIYQAHFILKSFALEKLNKFCQK", "aa_hexdigest": "33e870a6f6fda7495f53a55c9ab7412c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5536.572899999998, "isoelectric_point": 7.804816627502442 }, "id": "LMJBGEANGM_954", "locus": "GCA013368015_00952" }, { "type": "cds", "sequence": "contig_1", "start": 551270, "stop": 551491, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350", "UniRef:UniRef90_UPI001CC77350" ], "nt": "ATGGATATTAAACCCACAACAACAGGAAGTAATAGCGATAGTAGTAAAAACATTGAAATTCCAACACGTACCTTTGAAAATCACATGGTAGAAGATTCAATGAATACTCAAAATTTGCAACAATTAAGTTATAAAATAAAAGATCCTCAGAAAATAATTCCTAATGGAATTTATTCAGTTTTTCAAAGAGAAATGAATATTAAAAAAATATCAATACTTTGA", "aa": "MDIKPTTTGSNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQLSYKIKDPQKIIPNGIYSVFQREMNIKKISIL", "aa_hexdigest": "f9399e92917ae03e55337a45ad53773e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 551137, "stop": 551491, "observations": { "insertion": [ 551167 ], "mutation": [ 551218, 551272 ], "stop": [ 551157, 551218, 551272 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001CC77350", "query_cov": 1.0, "subject_cov": 0.6239316239316239, "identity": 1.0, "score": 142.0, "evalue": 2.31e-42, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MDIKPTTTGSNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQLSYKIKDPQKIIPNGIYSVFQREMNIKKISILWNFGILLLSVIAILLLYFWPNLFNFSNAKDFKIKWLDILFRQYL" }, "paralog": false, "identity": 0.9572649572649573, "score": 214.0, "evalue": 3.05e-73, "description": "Internal stop codon at 551157, 551218, 551272. Frameshift due to insertion around 551167. Nonsense mutation around 551218, 551272.", "psc": { "uniref90_id": "UniRef90_UPI001CC77350", "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001CC77350", "UniRef:UniRef50_UPI001CC77350" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8401.495899999998, "isoelectric_point": 8.030133628845213 }, "id": "LMJBGEANGM_955", "locus": "GCA013368015_00953" }, { "type": "cds", "sequence": "contig_1", "start": 551498, "stop": 552262, "strand": "-", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGGATATTCTTCAAGAAAGACTCGAAAGAGAATTTAATTTAGATATTATTGCTACTGCTCCTTCAGTTGAGTTTGTTGTAACACTAACTAATGGAGATGTTCAATATATTACAAATCCATCACTATTTCCAGATAGAAGTTATATAAAAATGATAGAGGAACCTTATATTAAGGCTAATATAATGTTAACCGAAGAATATTTGGGCCCAATTATGGAATTATGCCAATCTAAGCGTGGCAAATATGTAAATATTGAATATTTAGATGAAACCCGGAGATTATTAATTTATGAATTGCCTTTAAATGAAATAATATTTGATTTCTTTGATTTAATGAAGAGCTATTCAAAAGGCTATGCTTCATTTGACTATGACTACATTGGTTTAAGAGAAAGCGATTTAGTAAAGGTTGATATTTTATTAAATGGTGAGAAAATTGATGCCTTAGCAATGATTGTTCACAGAGATTCAGCATATAATAAAAGTAGAGCATTAACAGAAAAACTAAAGGAAGTAGTACCAAGACAAAATTTTGAAATTCCCGTTCAAGCTGCAATTGGTGCTAAAATTATAGCAAGAGAAACAATTAAGGCATATAGAAAAGATGTTACAGCTAAGTTATATGGTGGTGATGTTACAAGAAGACAAAAACTATTAAAAAAACAAAAAATAGGTAAAAAAAGGATGAAACAAATAGGAAGCGTTGAAGTCCCACAAGAAGCATTTTTGGCAATATTAAAAACAGATGATTCTCGAAAGGATTAG", "aa": "MDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD", "aa_hexdigest": "aebb4b468e6158986baae7c5c70c2a85", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8639455782312925, "identity": 0.6890000000000001, "score": 345.0, "evalue": 4.96e-117, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 551498, "stop": 552562, "observations": { "mutation": [ 552328, 552535 ], "stop": [ 552328, 552535 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 1.0, "score": 489.0, "evalue": 1.55e-169, "gene_start": 1, "gene_end": 254, "reference_start": 346, "reference_end": 599, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9915254237288136, "score": 684.0, "evalue": 1.32e-248, "description": "Internal stop codon at 552328, 552535. Nonsense mutation around 552328, 552535.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "LMJBGEANGM_956", "locus": "GCA013368015_00954" }, { "type": "cds", "sequence": "contig_1", "start": 552326, "stop": 552451, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGGATATAAAAAAATAAAACCAGTTGTTTATACTGGATTTTATCCCGTTGATACTAGAGATTACAATGATTTAAAAGATGCCCTAGAAAAAATTAGTTTGTCTGATTCTTCATTGGTATGA", "aa": "MPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLV", "aa_hexdigest": "4a49afffe937ca97bc817e164d8b878b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4667.291399999999, "isoelectric_point": 5.986134910583496 }, "id": "LMJBGEANGM_957", "locus": "GCA013368015_00955" }, { "type": "cds", "sequence": "contig_1", "start": 552533, "stop": 553297, "strand": "-", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGACAAACGAAAAAATAAGAAATTTTGCAATAATAGCACACATTGATCACGGTAAAAGTACTTTAGCTGATCGTATTTTGGAATTTACCAATACAATTTCCGCCAGGGAAGCAAAACCTCAAATTTTAGATTCAATGGAACTAGAACAAGAACGTGGAATTACTATTAAATTAAATGCTATTCAAATTAAATATAAAGATTACATTTTTCACCTGATAGATACACCAGGTCATGTTGATTTTACTTATGAGGTTAGTAGATCACTTGCTGCATGTGAAGGAGCATTGTTGCTAGTTGATGCAACTCAAGGAATTCAAGCACAAACATTGGCTAATGTTTATATGGCTATGGAAAATAATTTAGAAATCGTTCCTATTATTAATAAGGTAGATTTAATTAGTGCAGATCCTGAAAGAGTAAAAAAAGAGATTCAAGACATGATTGGAATTGATACTTCTGACGCAATTATGATTTCTGCAAAGACAGGATTAAATATTGATAAAGTAATTGATGCAATTATAAACAAAATTCCTGCTCCTAAAGAAGCAGATATAAATAAACCATTGCAAGCTTTAATTTTTGATAGCTATTTTGACAATTATCGTGGCGTTGTTATCTATGTGAGATTGTTTGACGGCAAAGTAAAAGTAGGCGACGAAATTTACTTTATGCAAACAAAAAAGCATTATTTAGTAACTGAATTAGGCGTTAAAAACCCTAATGAAGTTCAAAAAAATGAATTGCAAGCTGGCGAAGTTGGTTGA", "aa": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIDAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTKKHYLVTELGVKNPNEVQKNELQAGEVG", "aa_hexdigest": "db14056d5fb1586be72ee423a2d79036", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00317F3865", "query_cov": 1.0078740157480315, "subject_cov": 0.8827586206896552, "identity": 0.629, "score": 323.0, "evalue": 2.82e-108, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A0P1NWJ7" }, "genes": [ "lepA" ], "truncated": "5-prime", "pseudogene": { "start": 552230, "stop": 553297, "observations": { "mutation": [ 552328, 552535 ], "stop": [ 552328, 552535 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.992, "score": 489.0, "evalue": 2.2e-169, "gene_start": 1, "gene_end": 254, "reference_start": 1, "reference_end": 254, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9859154929577465, "score": 682.0, "evalue": 7.58e-248, "description": "Internal stop codon at 552328, 552535. Nonsense mutation around 552328, 552535.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "LMJBGEANGM_958", "locus": "GCA013368015_00956" }, { "type": "cds", "sequence": "contig_1", "start": 553398, "stop": 554885, "strand": "-", "frame": 1, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "nt": "TTGAATAAGAATCACTATGGACTTGAAAAGCCAAAGGAAAGAATTCTTGAATTTATTTCGGTTTTAACTCATCTTAAAATTAAGGAAGAAAAAACTGAATATTTACCAATAAAAGGAAATGATCAAGAATTTATTGATACTAAATTATTCGTTCATAAAACGGGTCAAACCACAAACAAACCAGTAAATAATATTCCTATTTTAACATTAATTGGTCCTCCAGGAACTGGTAAAACTACACTTGCTAAATCAATTGCTGAATCATTAAATCGTAGATTTATAAAGGTTTCATTGGGCGGTGTTAAAGATGAAAGCGAAATAAGAGGACATAGAAGAACTTATGTTGGGGCAATGCCTGGAAAAATAATATCAGCAATTAAAAAAGCCGGAGTTTCAAACCCAGTCATTTTGTTGGATGAAATAGATAAGATGTCATCAGATTTTCGAGGCGATCCAACAAGCGCAATGCTTGAAGTGTTAGACCCTGAACAAAATGTTAATTTTCAAGATCACTACTTGGATTTAGAATACGACTTATCAAAAGTATTATTCATCGCTACTGCTAACTACTATGATTCAATACCAGCAGCACTAATTGATCGTGTTGAATTGCTTGAACTTTCAACATATACTACTAAAGAAAAAGTAGAAATTGCTAAATCACATTTACTTCCAAAAGTGTATGAACAAAATGCCTTAAATCCTGAACAATTTCAAATTTCTGACGAAGTTTTAGAATTTTTAGTTCGCTACTACACAAGGGAAAGTGGAGTTCGTGAATTAAAACGTATTTTAGATAGTATAGCAAGAAAGATTGTTGTCGAAATTCTTGACAAAAAAATTAATGATAGTTTTGTTGTTACAAAAGAAGTTGTAATTGAATTTTTAGGACCAATTAAATTCGATGATGATGAAAACGAAAATAAAGCACAGATTGGTGTTGTTAATGGGTTAGCATATACAAGTTTTGGTGGTTCAACATTAGCTATTGAAGTTACAACATTCCCTGGAAAAGAAGGCTTAAAAATGACAGGGCAACTAAAAGATGTTATGCGTGAATCTGCTCAAATTGCATTAGCTTATGTAAGAGCAAATGCTGCCAAATTCAATATTGACTTTGATTTTGAAAATACAGCAATTCATATTCACGTTCCAGCAGGCGCTATTCCAAAAGATGGCCCAAGTGCCGGAGTAACATTCACAACCTCAATAATTAGTGCCTTATCTAAAAGACCAGTCCCTGCTAATATCGGTATGACTGGAGAAATTACATTGCGTGGAAAAGTTCTACCAATTGGTGGTTTAAAAGAAAAATCATTGGCAGCTGCTCAATTTGGTATTAAAAAGATTTTTATTCCATATGATAATTATAAGAATCTAAAAGATGTTCCTGTTGAAGTTAAAAATGTTGTTGAATTTGTGCCAGTTCATTATTATGACGAAATATTTGAACAAATATTTGGTGTAAAAGAAACAAAAAATAATTAA", "aa": "MNKNHYGLEKPKERILEFISVLTHLKIKEEKTEYLPIKGNDQEFIDTKLFVHKTGQTTNKPVNNIPILTLIGPPGTGKTTLAKSIAESLNRRFIKVSLGGVKDESEIRGHRRTYVGAMPGKIISAIKKAGVSNPVILLDEIDKMSSDFRGDPTSAMLEVLDPEQNVNFQDHYLDLEYDLSKVLFIATANYYDSIPAALIDRVELLELSTYTTKEKVEIAKSHLLPKVYEQNALNPEQFQISDEVLEFLVRYYTRESGVRELKRILDSIARKIVVEILDKKINDSFVVTKEVVIEFLGPIKFDDDENENKAQIGVVNGLAYTSFGGSTLAIEVTTFPGKEGLKMTGQLKDVMRESAQIALAYVRANAAKFNIDFDFENTAIHIHVPAGAIPKDGPSAGVTFTTSIISALSKRPVPANIGMTGEITLRGKVLPIGGLKEKSLAAAQFGIKKIFIPYDNYKNLKDVPVEVKNVVEFVPVHYYDEIFEQIFGVKETKNN", "aa_hexdigest": "741d1c578a92641f7b6b5d5af54a10d1", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0P0U7", "query_cov": 0.9838383838383838, "subject_cov": 0.9779116465863453, "identity": 0.86, "score": 821.0, "evalue": 8.28e-298, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A3B0P0U7", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0U7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0U7" ], "product": "endopeptidase La" }, "genes": [], "id": "LMJBGEANGM_959", "locus": "GCA013368015_00957" }, { "type": "cds", "sequence": "contig_1", "start": 554934, "stop": 555881, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTACCTTATATAGCAATAAAAAATCAGATTATTATTCCATATACTTCTGACAAAATTAGAGTTGGAAAAGCTAAATCAGTGTCAGCTTTATTAAAGGCTTTTAATGGAAATAAAAATATAGTTATTACATTTTTAAATCCAAATTATAAACAATCAAATGAGGAACATACAATTAATGATATTGCTCCTGTAGGCGTACTTGTTAATATTACTAACATTAGTGAAGAATCAGGAATTTATCATGTTAATTTAGAAGCAAAAGAACCGGTTAATATTAATAAAATCTCAATAGTTGATGAAAAATCAACAAATAGTGAGGATAATTTTTATGTTGATATTTCGCCTATTCAAGTCAATTCATATGATTCATATTTACCAAAAATTGAAGAAATAAGACAAAAATTCTTCTCACTATCTAAACAAGTTATTAACGAAGGTATAGGAACAGTTTTATTTCGAGATTTAGAAAAAGAATTTGAAACTAACAAACCTAAACAATCATTAGTTGAATATGAAACTGAATCATGGCAACTTATAAAGTTTTTGAAAAAGTACTTTTTGAGTGATGAACTTCTTTCTTTAATTGAAAGCAATGACGTTTTAGCTAGATACAATGAACTAATTAAAAAGGCAAATAAACAAGTTCAATTTGAAAAAATAGAAAACGAAATTAATAGTAATTTACGTGAAGATATGGAAGACCAACAAAGAGATTTCTTCCTAAGAGAAAAAATGCGTCAAATTAGAAAAATGTTGAAGGATGATGAAGGCGGAAAATCTGCTGACGACATTGAAAAAGACAGCACTTTAAAAGAACAATATCCTGAATATGTTCTAGAAGCATTAAGATCCGAACAATCAAGAATGGCCTCAATGATGCCATCTAGCCCGGAAGCTAATATATCTAAAACATATATTGATTTAATCTTGGCATTGCCTTGA", "aa": "MKLPYIAIKNQIIIPYTSDKIRVGKAKSVSALLKAFNGNKNIVITFLNPNYKQSNEEHTINDIAPVGVLVNITNISEESGIYHVNLEAKEPVNINKISIVDEKSTNSEDNFYVDISPIQVNSYDSYLPKIEEIRQKFFSLSKQVINEGIGTVLFRDLEKEFETNKPKQSLVEYETESWQLIKFLKKYFLSDELLSLIESNDVLARYNELIKKANKQVQFEKIENEINSNLREDMEDQQRDFFLREKMRQIRKMLKDDEGGKSADDIEKDSTLKEQYPEYVLEALRSEQSRMASMMPSSPEANISKTYIDLILALP", "aa_hexdigest": "cfc975615ccdee17fa2a746628635cca", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 36423.99790000007, "isoelectric_point": 5.119624519348144 }, "id": "LMJBGEANGM_960", "locus": "GCA013368015_00958" }, { "type": "cds", "sequence": "contig_1", "start": 555948, "stop": 556736, "strand": "-", "frame": 1, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "KEGG:K03687", "RefSeq:WP_012855682.1", "SO:0001217", "UniParc:UPI0001BC2428", "UniRef:UniRef100_D1J8J6", "UniRef:UniRef50_D1J8J6", "UniRef:UniRef90_D1J8J6" ], "nt": "ATGAGTCAAAATATTTCAAAATATGATTATATAGAGTGTATTAAAAAAATTAACAAAAATGAAAAAATTGAATATAAGGGATTTGTTGGCTTTGATTTAGATAAAAATATTGAAAAAACTATCTTGGAAGGAAAGCAAAACGAAAAAGGTGAGTTAGTTTTAAAAATTGATAATGAAATTTTTAAAGTAATAATTATTGATTTTCAAAAGACAAGTCCTAAGTATTTAGAAGTTTTTGCTAAAAATCAAGAATTAAATGAAAATATTAAGAAATTGCAACTAAATTTTTTGGAATTAGGCGAACTTAATGAAAAAATAAAACAAGAAAAGACACAACAAGAAATTTTATTTAAAAATCAAGTAATTGAATTGGAAGCAAAAGCACAATCAAAAATAAATGAACATCGTCAAAAAAATGATGAGCATTTATTACAACAAAAAACAGAATTAAAAAAATATGCTCTACAAGACTTTTTAGAAGAGTTCATTAAGATATATACAAAATATGACAGTGCTTTAAATTTTGCAAAAAAATCTGATAATATCGCTGTTAATAATTTTGCAAAAGGCTTTGATATGCTTAAGAATGATTTTGAAAATTTAATGTTAGATAATGGAATAAAAATAATTGAACCAAAAGTAGGAGATTTATTTGACCCTGAATGTCAACAAATTACTGAAAGCATCGAATCAAAAGAACCCTCTGGCACAATTCTTGAAGTAAAGAGCAATGGTTATTCATTATTTAACAGAATTTTAAAACCCGCCTCAGTAATAATTAGTAAATAA", "aa": "MSQNISKYDYIECIKKINKNEKIEYKGFVGFDLDKNIEKTILEGKQNEKGELVLKIDNEIFKVIIIDFQKTSPKYLEVFAKNQELNENIKKLQLNFLELGELNEKIKQEKTQQEILFKNQVIELEAKAQSKINEHRQKNDEHLLQQKTELKKYALQDFLEEFIKIYTKYDSALNFAKKSDNIAVNNFAKGFDMLKNDFENLMLDNGIKIIEPKVGDLFDPECQQITESIESKEPSGTILEVKSNGYSLFNRILKPASVIISK", "aa_hexdigest": "540a0f6145f0fd1ae69712bb9e769df0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2428", "ncbi_nrp_id": "WP_012855682.1", "uniref100_id": "UniRef100_D1J8J6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2428", "RefSeq:WP_012855682.1", "UniRef:UniRef100_D1J8J6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8J6", "uniref90_id": "UniRef90_D1J8J6" }, "psc": { "uniref90_id": "UniRef90_D1J8J6", "gene": "GrpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_D1J8J6", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_D1J8J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J6" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "LMJBGEANGM_961", "locus": "GCA013368015_00959" }, { "type": "cds", "sequence": "contig_1", "start": 556956, "stop": 557789, "strand": "-", "frame": 1, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "SO:0001217", "UniRef:UniRef50_B3PN01", "UniRef:UniRef90_D1J8J7" ], "nt": "ATGGAGCAAAATTCTTCTAATGCAAACACAAATAAGTCTTTAATTATATGCAATAATTATGATAAGAAACTAATCAAATATTTTAGATACATTGTTGAAATGTACATTAAAACCGGCGAACCAGTAGGATCAAAAAGATTAGTTGAAAAATTTAAATTATCTTGTAGCTCTGCAACCATAAGAAACGCAATGGCAGAATTAGAAAAACTAGGTCTTTTAGAAAAAAAACATGTTTCAGGGGGAAGAATTCCTTCTTCAAAAGGTCTTGAATACTATACAAAAAATTTACTTTATAATGCTAAAAAATATTTCAATGAAAAACTTGAAGATCTTTTATCTAAGCGTAGGTTAAGCATCAACACAACATTAGAAGAAGCAGCAAATGTCGTTAGCCAAATGGCAGGGTTTGCCGTAGTTGCAACTTCAAACAATGAATCTGAATTATTGAAAAGTATTCAGTTGACACCGCTTGATGAAGAATCTGCAGTTGTTGTTATTGTTACAAGTTCAGGAAATGTTCAAAACAAAATGTTTAAGTTTGAAAGTGGGATTGAATTAAATGATTTAAGGATTGCAATTAGATTATTTAAAGAAAGATTGATTGATACTCCATTAATTAATCTTGCTAAAAAAACTGAATCATTAGCCCCAATATTATCTACACAGGTTAAACATTTTGAATTAATAATGCAGAAATTTATCAAAAATATTTTTTGTTTTGAAGAAAAATTGGAGAATAAATCGTTTAATAAAAATGTAATAATATTATCAGAACAAATAAGCAGAGAAGAGATCGCTAAGACATTAGAATTAATTGAAAACCACTCAGTTTGA", "aa": "MEQNSSNANTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSV", "aa_hexdigest": "43d9cde88eba5c18e7a2e99669cbf04c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 556737, "stop": 557789, "observations": { "mutation": [ 556958 ], "stop": [ 556958 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J7", "query_cov": 1.0, "subject_cov": 0.7914285714285715, "identity": 0.993, "score": 517.0, "evalue": 8.46e-184, "gene_start": 1, "gene_end": 277, "reference_start": 1, "reference_end": 277, "reference_sequence": "MEQNSSDTNTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSVWEAIENNLDEEKNIKLDFSHPNLNIISKRIQFINHKNIKEISILGPKNLDVEESLEAINLFEGIIKKEEEKGE" }, "paralog": false, "identity": 0.9914285714285714, "score": 665.0, "evalue": 1.46e-244, "description": "Internal stop codon at 556958. Nonsense mutation around 556958.", "psc": { "uniref90_id": "UniRef90_D1J8J7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_B3PN01", "cog_id": "COG1420", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J7", "UniRef:UniRef50_B3PN01", "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN01" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "LMJBGEANGM_962", "locus": "GCA013368015_00960" }, { "type": "tRNA", "sequence": "contig_1", "start": 557916, "stop": 557992, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 76.1, "nt": "GCGCCCGTAGCTCAATTGGACAGAGTGTTTGGTTACGGCCCAAAAGGTTGCGGGTTCGACTCCTGCCGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 557956, 557958 ], "id": "LMJBGEANGM_25", "locus": "GCA013368015_00961" }, { "type": "tRNA", "sequence": "contig_1", "start": 557995, "stop": 558069, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 67.3, "nt": "GCCACCATAGCCAAACGGCCAAGGCATAGGTCTGCAACACCTTGATTACCGGTTCGAATCCGGTTGGTGGCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 558044, 558046 ], "id": "LMJBGEANGM_24", "locus": "GCA013368015_00962" }, { "type": "cds", "sequence": "contig_1", "start": 558270, "stop": 558446, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCGAACGTGAACAAAAAAATAAAATTACTTTTATGAGCTCGCCTATTGATTTTACTTCTTCAAAGCAACATATAATATTTAATAAAATGCCTGGTAATGATGGTATAACTAGGATTGAATCATTGCTTAAATTGACAGTCTTAAGATTGATGAAAAAGTTCATTTCGTTCTAA", "aa": "MSEREQKNKITFMSSPIDFTSSKQHIIFNKMPGNDGITRIESLLKLTVLRLMKKFISF", "aa_hexdigest": "0cf809e788642deac2151fcdf9a8dd95", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6776.965699999999, "isoelectric_point": 10.11536350250244 }, "id": "LMJBGEANGM_963", "locus": "GCA013368015_00963" }, { "type": "cds", "sequence": "contig_1", "start": 558471, "stop": 558791, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATACAAACCAATCATTGGAAAAATTTGCACCAGAACTAAAAAATGAAATCAAAGATATTACTTCAATGAAATCAAATCAAGGGTTATTGCTCATTGATCAAAATCTCGTTAATGCAAGAAATATTTTAAAATTTTTAGCTTTCTTATTTGGCACTAAGAAAAACAAAAGTGTAGCATTTGTTGACAACAATAATTTGTTTGAAGTCATGTCTAATAATTTAAAAAACTCATCAGAAATTAATTCAATTGTTCAAAATTTGATTTCTGTTGATTATTTATTCATAGATCGCTTTGCGATCGGAGTAAAACCTGAATGA", "aa": "MNTNQSLEKFAPELKNEIKDITSMKSNQGLLLIDQNLVNARNILKFLAFLFGTKKNKSVAFVDNNNLFEVMSNNLKNSSEINSIVQNLISVDYLFIDRFAIGVKPE", "aa_hexdigest": "183b3c99128504fb27e3f916375697ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12025.706099999989, "isoelectric_point": 7.965213966369628 }, "id": "LMJBGEANGM_964", "locus": "GCA013368015_00964" }, { "type": "cds", "sequence": "contig_1", "start": 558873, "stop": 559172, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAATATTTGATGAATTGTTTAAAAATTCTCTTTTGAAAAATATTGATTTAGAGGCTGAAAAAGCAAAAATTTTAGCCGATCCGAATGTTAAAGCAATTATTGAAAAACTGAATTTAAATTCTAAACAAATCCAAAAAGGAATGAATTATTTGCAAAAATATTATAGCTATATTGAAGCACATAAACAACTTCCAACATGGACCCTCTTTGTTGATGATAATGGAATTTTGCAAATTGATTTAAGCAACGATGGTTTTTTTAGAAAACAAAAAGAAATGAATAACTTTTGA", "aa": "MENKIFDELFKNSLLKNIDLEAEKAKILADPNVKAIIEKLNLNSKQIQKGMNYLQKYYSYIEAHKQLPTWTLFVDDNGILQIDLSNDGFFRKQKEMNNF", "aa_hexdigest": "6422acb5303545209bb72792c13b15f6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11672.28969999999, "isoelectric_point": 6.571974372863769 }, "id": "LMJBGEANGM_965", "locus": "GCA013368015_00965" }, { "type": "cds", "sequence": "contig_1", "start": 559201, "stop": 560097, "strand": "-", "frame": 3, "gene": "dnaB2", "product": "DnaD domain-containing protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_D1J8J9", "UniRef:UniRef90_D1J8J9" ], "nt": "ATGGTTGAAAATTTTTTTATTGAAAGCGAGAGGGAAATTACACAAAACGATTTTAAAAATCTAGTAATTTTTTATGCTCCGATTTTAGGCCATAGTAGCATATTTCTATATCAATTTTTATATAATTTAAAAAATATTTATACAAAAAATAAACTTTTTAATTTTTCTGAAATTAAAGATATTTTATTATTAAATGAACAAGAATTTGAGGAATGTCGTAGTAAACTAGAAGCGCTAAGCTTACTAAAAAGTTATTTAAACAAGGATGGTGTTTATATTTTTAGTTTAAATGCTCCTTTGAATGCAAATGAAATAAGCAATAATAAAATTATTTCTAATATGTTAATTAAATTAATCACTAAATCAAAATATGAAGAATTATTAAAATTAAATGCAAATTATCAATTTGATAAAACAACAATTAATGATGTTTCTAAAAAATTTTATGAAGTATTTAATCTAGAAAATATAATTGATTTAAATGCAAAAAATGAAGAATTGAATTTTAATTCAATTACTCTAGATGAATTAAAGGACAAGATAGAACCCGAACAATTTATTAGTCAATATACAAAAACAAATTTAAGTTTTAGCCAAAATGAAATGATTAGAAAATTGAAAAGAATGAAATTCAATAATTTTTCAATAAATGCCTTCATTTATTATTCAATTAAAATTAACCATTCAGTTGTATGCAAATATATTGAAAAAATAGCCGAAGATTTTAGTCATAGAAATTTATATGATGCTGAAAGAATTGATCAAGAATTAGAAAATGTTTATCTAATAAAACAAAATGCAAATAAAGAGTTAAAAAATATCAAAAAAAATGAAGATGAATCAGTTACTAGAGAAGATTTAAATTGTTTTTTAGATCTCGCTAATGAACATGATTGA", "aa": "MVENFFIESEREITQNDFKNLVIFYAPILGHSSIFLYQFLYNLKNIYTKNKLFNFSEIKDILLLNEQEFEECRSKLEALSLLKSYLNKDGVYIFSLNAPLNANEISNNKIISNMLIKLITKSKYEELLKLNANYQFDKTTINDVSKKFYEVFNLENIIDLNAKNEELNFNSITLDELKDKIEPEQFISQYTKTNLSFSQNEMIRKLKRMKFNNFSINAFIYYSIKINHSVVCKYIEKIAEDFSHRNLYDAERIDQELENVYLIKQNANKELKNIKKNEDESVTREDLNCFLDLANEHD", "aa_hexdigest": "27e5d497337c5507be98ac98a34c43d2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8J9", "query_cov": 1.0, "subject_cov": 0.9900332225913622, "identity": 0.987, "score": 565.0, "evalue": 2.95e-203, "valid": true, "gene": "DnaB2", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_D1J8J9", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346" }, "pscc": { "uniref50_id": "UniRef50_D1J8J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J9" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaB2", "dnaD" ], "id": "LMJBGEANGM_966", "locus": "GCA013368015_00966" }, { "type": "cds", "sequence": "contig_1", "start": 560410, "stop": 560760, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATTAACCTTCACTTAAATACTATATTTTCTTTTTTAGAAAGTACAATAACCATTAATGATTTTATTCAAGAATTACTAAATAATAATATAGAGTATTTTGGTATTACAGAACATTGCAATTTTTATAGCATGCCACATTTTTTAAATGAAGCTAGAAAACATGATTTAAAGCCTATTTTTGGTCTTGACGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTATGGAAAAAAAGGCTATATAGATTTAGCTGAAATTAGAGATTATTCTAATTTAATATGA", "aa": "MKLINLHLNTIFSFLESTITINDFIQELLNNNIEYFGITEHCNFYSMPHFLNEARKHDLKPIFGLDVNVKIENNLYRFIVYCQNKESFMNLKMLSLNYGKKGYIDLAEIRDYSNLI", "aa_hexdigest": "a61b39fa76280bbb40b856b4059e5b5d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13791.777399999983, "isoelectric_point": 6.044451713562012 }, "id": "LMJBGEANGM_967", "locus": "GCA013368015_00967" }, { "type": "cds", "sequence": "contig_1", "start": 560944, "stop": 561201, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAATATTTCAAATAATAAAATATTTGAAAGCATTTACGAAGAATTTAAAGTTGATATTGAAACAAAAAATAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAAAGGTTTCAAAATGCCCAAATTCAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTTGATAAATATTCATGA", "aa": "MSNISNNKIFESIYEEFKVDIETKNNSKLEELIERTNNFAKKLYFEIETKGFKMPKFKNDKNIASNEYLKLLLFKSIREKFDKYS", "aa_hexdigest": "2fe490ac1564df731dbce3c4aaae4b4c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10257.658699999989, "isoelectric_point": 9.072136116027831 }, "id": "LMJBGEANGM_968", "locus": "GCA013368015_00968" }, { "type": "cds", "sequence": "contig_1", "start": 561445, "stop": 563400, "strand": "+", "frame": 1, "gene": null, "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023586AB7" ], "nt": "ATGCCTGATATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTCGTGGCAAATATTGTTACTTTTTCTTCACTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTATTAGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCATTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACACATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCAATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGCATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAGACATTGACAACTATTTCAAATATAGTTGATCTAATTTATCAAAATCAAAATCTTAAAATTGACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAATTATTGAGCTCTGGCCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGACAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCAGGTCCAATGAAAAACGTTTCTGTTTATGCAAAAAGAAAATTTGGACTAGAGTCGATACCAAAAATAAATAATGAATATGATAAAATCCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATTATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCTGACAATCTAAGAAGAATTATCTCTAAGAAAAAAATAAATGAAATGGAAGATATTAAATCTATTTTTATTGAAAGAGCAATGAAGAATGGCTATGAAAAGTCTTTGGCCACGAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTCAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCATTACCCCTTAGAATTTTATACGTCATGTATAAGCTCGGCACACGGCTCTCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGTGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAAGCTATAATTAAAAATAGAAAAATTATATTGCCACTTACACTAATTAAGGGTCTTGGTCCCGAAATTGCTAATTCAATTGTTGAAAATAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTACTAATAAAAGCAAACACTCTTAGATCATTTAATTATAATCAAAACACCTTGTTGAATGAGATTCAAAAAAACAATACTGATACAATGTTATTTATAAAAATGTTTAAGAATAACATTGAAGGTGTTAGCTCTAATATAATAAACTCTTATAAACCAAGTGAAATCCTTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTGTTAGGTCAAGTTTATAATTTTAATTCAAGCGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATTGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATGTTAAGGGAAAAACTATCAAAGTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAA", "aa": "MPDIDIDVQDNKRNDVIEYLLKKYGHEFVANIVTFSSLGKKSAIRDILRINNIAPTLINEISKNIENDEQDLLVEYNENRNLQLSLAKLNPNDESFSTRILNQAARISGFYRQIGTHAAGLVISDQPVISIAPVQYNSENFLQTQISMEYLEDFGLVKMDILGLKTLTTISNIVDLIYQNQNLKIDLKDIDLTDQKTLELLSSGHTRGVFQVESPIMIKALRQVKVNNFEDIVAIISLNRPGPMKNVSVYAKRKFGLESIPKINNEYDKILDSTYGIIVYQEQIMKIAQVVAKMTFIEADNLRRIISKKKINEMEDIKSIFIERAMKNGYEKSLATNIFNSIEKFASYGFNRSHAVSYSIITFQMAFLKAHYPLEFYTSCISSAHGSQDTIAKFVNEASECGVEIVSPDINLSESEAIIKNRKIILPLTLIKGLGPEIANSIVENRSKYGPYKNFLDFLLKISSSQSIGKSTIELLIKANTLRSFNYNQNTLLNEIQKNNTDTMLFIKMFKNNIEGVSSNIINSYKPSEILNQDIMFEKKNEIELLGQVYNFNSSASNGMPGQTSFIDMHVGNEYLAIVNCTNVKQKISKKGNNYFELDLEANMQKIRAFYFTSNQEILNVKGKTIKVTLEKRNENIFYLRSIKEVLNEER", "aa_hexdigest": "28d00bf40cafb395df4484e640e86195", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0023586AB7", "query_cov": 0.9815668202764977, "subject_cov": 0.8753424657534247, "identity": 0.507, "score": 589.0, "evalue": 2.24e-200, "valid": false, "product": "DNA polymerase III subunit alpha", "uniref50_id": "UniRef50_UPI0023586AB7" }, "pscc": { "uniref50_id": "UniRef50_UPI0023586AB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023586AB7" ], "product": "DNA polymerase III subunit alpha" }, "genes": [], "id": "LMJBGEANGM_969", "locus": "GCA013368015_00969" }, { "type": "cds", "sequence": "contig_1", "start": 563387, "stop": 564280, "strand": "+", "frame": 2, "gene": "exoIX", "product": "DNA polymerase I", "db_xrefs": [ "COG:COG0258", "COG:L", "RefSeq:WP_020002546.1", "SO:0001217", "UniParc:UPI0003651CA3", "UniRef:UniRef100_A0A2K9YTX0", "UniRef:UniRef50_A0A318U4A1", "UniRef:UniRef90_D1J8K1" ], "nt": "ATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCCTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCAGAAGGCTTTGAAACCAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATTTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCGCCAGAAAGTTTTTATCTACAGCTTGATTTAATTCAAGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAAGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAATTAATAGATGAACAAACAGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTACCAAATCAAGTTATTGATTATAAAGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGTCCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACATTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAGAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTTTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAA", "aa": "MKKDNEQLLIIDGTFLAYRSYYATSYNATQILTTSEGFETNAIVAFFKTLFLLLKKLNPKYIYIAFDSHVKTFRHELFPSYKDGRKKAPESFYLQLDLIQEILTNINIQNQYYNGFEADDIIAKVKNKFNLNTIIFSGDQDLNQLIDEQTAIIKKDKTGSFFFLNSKNFSEYYDFLPNQVIDYKALVGDNSDNFKGVVGIGPKSAKTLLEDYKTIENIYNNIEKIKPSIAKKLMEYKENTFLDKYLATLRIDFDLQLPELENLAISNIAISNSAKQIIDKFELYSLENSLKNLINKK", "aa_hexdigest": "a6b2523fe6605cc4d24d3bfc903ae80d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0003651CA3", "ncbi_nrp_id": "WP_020002546.1", "uniref100_id": "UniRef100_A0A2K9YTX0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003651CA3", "RefSeq:WP_020002546.1", "UniRef:UniRef100_A0A2K9YTX0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTX0", "uniref90_id": "UniRef90_D1J8K1" }, "psc": { "uniref90_id": "UniRef90_D1J8K1", "gene": "ExoIX", "product": "DNA polymerase I", "uniref50_id": "UniRef50_A0A318U4A1", "cog_id": "COG0258", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_A0A318U4A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U4A1" ], "product": "5'-3' exonuclease, exo domain of DNA polymerase I" }, "genes": [ "exoIX" ], "id": "LMJBGEANGM_970", "locus": "GCA013368015_00970" }, { "type": "cds", "sequence": "contig_1", "start": 564307, "stop": 564678, "strand": "-", "frame": 3, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "TTGCCAGCAACAACAAAAATTTTAACTTTTGGGAAGTCAAAAAAAGGAATAATAAAACTTAGAGATGAATTATTAAATGCTTTTCATGATATTTCAACACATATTGTATCAAAAGGATATTCAATTGAAATTAGCGATATTAATGCTAATAAAGGAATTGGCGATCGATTTGTATGTGATTTGTTAAATATTGATTATAAAGAAGCAGTTCACTTAGGCGATTCGGGCAATGATGTTGTTACTCAAAAATATTTAGGTGGCTTTGTAGCGATGAAAAATTCTACAAAGAACATAAAACAAAAAGCAGATTATGTAGGATATTCATATAAAAAATCTGGAGTAGCAAAAACATTGCAAGAACTTCAAAAATAA", "aa": "MPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTQKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK", "aa_hexdigest": "e2821e4ba0226feb7075948f6be44d6e", "start_type": "TTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 564307, "stop": 564978, "observations": { "mutation": [ 564723 ], "stop": [ 564723 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.458955223880597, "identity": 0.9840000000000001, "score": 240.0, "evalue": 3.49e-78, "gene_start": 1, "gene_end": 123, "reference_start": 146, "reference_end": 268, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9910313901345291, "score": 435.0, "evalue": 1.43e-157, "description": "Internal stop codon at 564723. Nonsense mutation around 564723.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "LMJBGEANGM_971", "locus": "GCA013368015_00971" }, { "type": "cds", "sequence": "contig_1", "start": 564721, "stop": 565113, "strand": "-", "frame": 3, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "ATGATTAATTTTAAACCTAAGGCATATTTTTTGGATATGGATGGCACATATCTAGATTTGCCTCGCAAAAAACAAATTATGATAAGCGAAAAAAATGTTGAGGTTGCTAAGGACTTCAATAATAGAGGAATCCCTGTTATTTTGTCAACAGGTAGAGGAAATTCAAAGTTTGTTTTGGATTTAACTAATAAAATAAATGCACCGTTTGCTGTTTGCCAAAATGGTGGAGTAATTGTAGATAAAAATAATAATGTTTTGAAACGCCATGAAATTAACCCTGATACATTGAAATCAATAATTGAAATTTTAATTGCACAAAAAATGTTTTTTATTTTTAATAGTGGCGATACAATATATGGAACAAAAACAAAATTTAGAATCGCTAGAGCTTGA", "aa": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARA", "aa_hexdigest": "8c27f040cf025701d81bd45bddccd7be", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 564418, "stop": 565113, "observations": { "mutation": [ 564723 ], "stop": [ 564723 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.48507462686567165, "identity": 1.0, "score": 261.0, "evalue": 1.44e-86, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9913419913419913, "score": 454.0, "evalue": 4.59e-165, "description": "Internal stop codon at 564723. Nonsense mutation around 564723.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "LMJBGEANGM_972", "locus": "GCA013368015_00972" }, { "type": "cds", "sequence": "contig_1", "start": 565121, "stop": 565327, "strand": "-", "frame": 2, "gene": null, "product": "Thioredoxin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "nt": "ATGATTGGCCCAGAATTAGAAAAATTAGCAGAAGAAAATTCAGATGTTAATGTTTTTGATTTGGACGTAGATAAATTCATTGATTTAGCAAGAGAAATGAATGTATCTCATATTCCATCGCTATTTTATTATAGAGACGGTAAGTTAGTTTCTCAAAGTGTAGGATACTTGCCAGTTGAAGAAATTTTAAACAATTTAAATAAGTAG", "aa": "MIGPELEKLAEENSDVNVFDLDVDKFIDLAREMNVSHIPSLFYYRDGKLVSQSVGYLPVEEILNNLNK", "aa_hexdigest": "b8d7bd8624b1522d9c36828a90a78435", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P2T3", "query_cov": 0.8382352941176471, "subject_cov": 0.9193548387096774, "identity": 0.544, "score": 72.8, "evalue": 1.09e-15, "valid": false, "product": "Thioredoxin", "uniref50_id": "UniRef50_A0A3B0P2T3" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P2T3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "product": "Thioredoxin" }, "genes": [], "id": "LMJBGEANGM_973", "locus": "GCA013368015_00973" }, { "type": "cds", "sequence": "contig_1", "start": 565343, "stop": 565507, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACTTGTTTTTGAACTAATAGAAAAACAAAAAGATTTGTATTATTTGCAAGAGCATATTAAAGGAGAAAATATTATGTATAAAACTATTGAATCAGTTGAAGAATTCAAAAAGAATGAATATTTAGAAGGAAAATCAATTGTTGCATTTAGAGCAGTTTGA", "aa": "MILVFELIEKQKDLYYLQEHIKGENIMYKTIESVEEFKKNEYLEGKSIVAFRAV", "aa_hexdigest": "e3c34a065d1b30a44f763fd2991c67c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6499.5041, "isoelectric_point": 5.297132682800293 }, "id": "LMJBGEANGM_974", "locus": "GCA013368015_00974" }, { "type": "cds", "sequence": "contig_1", "start": 565491, "stop": 565613, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGTATGCTGCATTTGATGGTAACGGTGATGATGAAGTAAAAATTAAAGAAGAAACAGGGGCATCAACAAGGTGTATTCCATTCAAAGAACCAATAAAAGTTTCAAATGATACTTGTTTTTGA", "aa": "MYAAFDGNGDDEVKIKEETGASTRCIPFKEPIKVSNDTCF", "aa_hexdigest": "79c1090abc86c7c2c3a94b405e703492", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 40, "aa_cov": 0.95, "hmm_cov": 1.0, "evalue": 6.839137373512148e-11, "score": 37.764034271240234, "start": 3, "stop": 40 } ], "seq_stats": { "molecular_weight": 4427.896500000001, "isoelectric_point": 4.507070732116699 }, "id": "LMJBGEANGM_975", "locus": "GCA013368015_00975" }, { "type": "cds", "sequence": "contig_1", "start": 565614, "stop": 566066, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGACTCATGGTGATGATCGAGGAATAATCATTCCACCAAAAATAGCAAGGACTCAAGTTGATATATTAGAAATTTTTAATTCAAAGGATGAACGAGTTCACAAGCTTGCCCTTGAATTAAAAGATGCATTATTAGCAAAAAATATTGTTGCAAACATAGATTCTTCAAACAAAGGTCCAGGTTTTAAAGCAGCAAATTCAGAAATTCATGGTACACCATTGAGAATAGAAATTGGTCCAAAGGATATTGAAAATAATAGTGTATTATTCGTTAGACGTGATAACCTAGAAAAAATTACTTCAACAATTGCTGAAGCAGCAGATATTGCCAGCTTCTTATTACAAAAAATACAAGACAATTTATTTGAAAGTGCTAAACAACGCTTACAAAATAATATTGTAACTGTTAATAATTATCAAGAATTTAAGGAAGCAATTGCTGCGGGTCATTGA", "aa": "MTHGDDRGIIIPPKIARTQVDILEIFNSKDERVHKLALELKDALLAKNIVANIDSSNKGPGFKAANSEIHGTPLRIEIGPKDIENNSVLFVRRDNLEKITSTIAEAADIASFLLQKIQDNLFESAKQRLQNNIVTVNNYQEFKEAIAAGH", "aa_hexdigest": "1c83bbcaacb986cdc68343a2b98df5fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 83, "aa_cov": 0.5533333333333333, "hmm_cov": 1.0, "evalue": 6.66604373538555e-16, "score": 53.8438720703125, "start": 27, "stop": 109 } ], "seq_stats": { "molecular_weight": 16654.736999999986, "isoelectric_point": 6.082818031311034 }, "id": "LMJBGEANGM_976", "locus": "GCA013368015_00976" }, { "type": "cds", "sequence": "contig_1", "start": 566100, "stop": 566345, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACAAGGTTTATAAAGATGTTATTGAAGAAAATTTAGCAATTCCAGTAGTAGAAGGCTGCAAAACGGATCATGAAAAATTTGCTGGCGCTGATATAACATACACCTTAGAATCAATGATGAAGGACGGAAAGGCACTTCAATCGGGTACTAGCCACTATTTGGGACAAAATTTTACTAAGGCATTCGATATAACATTCAAAAATAATGAAAATAAATTAATTAATCCTTTTGGTACCAGTTGA", "aa": "MHKVYKDVIEENLAIPVVEGCKTDHEKFAGADITYTLESMMKDGKALQSGTSHYLGQNFTKAFDITFKNNENKLINPFGTS", "aa_hexdigest": "9f9f35b3fc6881aaeb1e7cdccceaac90", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9072.162599999992, "isoelectric_point": 5.837330436706543 }, "id": "LMJBGEANGM_977", "locus": "GCA013368015_00977" }, { "type": "cds", "sequence": "contig_1", "start": 566466, "stop": 566717, "strand": "-", "frame": 1, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "ATGGATATTGAATTTAAAAAGAGAGGCGTTCAAAACGTTTATTTACCTCTTTTAATACCAGAAAGCTTATTACAAAAAGAAAAAAATCATGTTGAAGGATTTAATCCTGAACTTGCAACTGTTACTGAAGTAGGCGGCAAAAAATTGGCAGAAAAGTTTTTTATAAGGCCAACTTCAGAAACATTGTTTGCTGATTTATTCAAAAATGAAGTTTCATCTTACAATGATTTGCCAATAATTTATAATCAATGA", "aa": "MDIEFKKRGVQNVYLPLLIPESLLQKEKNHVEGFNPELATVTEVGGKKLAEKFFIRPTSETLFADLFKNEVSSYNDLPIIYNQ", "aa_hexdigest": "be7654839c9a3e753250bfb8135032dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.614, "score": 102.0, "evalue": 4.5e-27, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_978", "locus": "GCA013368015_00978" }, { "type": "cds", "sequence": "contig_1", "start": 566936, "stop": 567331, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGTTGCAATTGACATTGCTTCTTCAAATACCAAAAACCAATTTGTATTTGTAAACCTTGTTCAATTTGACAGCCATTTTGGACATAGAGAAGACCCAATAGGATATTCACAAAATATTTCAAGGCTAGATATTAAATTAACAAAATTGATAAACGCAATGAAATCAGATGACTTGTTAATTATAACTTCTGACCATGGAAATGACCCAACAAAAGGCCCTGATCATACAAGAGAAGCATTGCCATTGACAATATTTTCAAAGAAATTCAAATCACCATGTGTATTTAAAGAACCTTTGAAAGGCTTGGGAACTGCAGGAAATATTGTTGCAAGAAATTTCAATGTTCCAACCGTTCAAACAGGTGAAGATATTTTTGATAAATTAATCTAA", "aa": "MDVAIDIASSNTKNQFVFVNLVQFDSHFGHREDPIGYSQNISRLDIKLTKLINAMKSDDLLIITSDHGNDPTKGPDHTREALPLTIFSKKFKSPCVFKEPLKGLGTAGNIVARNFNVPTVQTGEDIFDKLI", "aa_hexdigest": "efe568986058dda60c7d493c057c6d40", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14543.415699999978, "isoelectric_point": 6.397819709777832 }, "id": "LMJBGEANGM_979", "locus": "GCA013368015_00979" }, { "type": "cds", "sequence": "contig_1", "start": 567551, "stop": 567853, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTTATACAAAAGTTCCAAACCCACAATTCATTGAAAAAGGTTTCCCTGCTGATTTAGTCGCAGAATTATCAAAAGCGTTTGATAATAGAAAAATTATTGGAAATAGAAATGCATCAGGCACAGTTATATTGGCCGAATTAGGTTTGCAACACATTGAAAAAGGAGATATGATAATTTACACTTCTCCTGATTCGACATTGCAAATTATTGGGGATGAAAAAACATTGGGTGTTGAAAAACTAACTGAATATGCAAAGAGAGCAAGAGCAATTTGTTCATCAAGACCTGAATGA", "aa": "MMGIYTKVPNPQFIEKGFPADLVAELSKAFDNRKIIGNRNASGTVILAELGLQHIEKGDMIIYTSPDSTLQIIGDEKTLGVEKLTEYAKRARAICSSRPE", "aa_hexdigest": "5d61518090ed1c2deb07abf8c68a2cfe", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11024.595599999986, "isoelectric_point": 6.585217857360839 }, "id": "LMJBGEANGM_980", "locus": "GCA013368015_00980" }, { "type": "cds", "sequence": "contig_1", "start": 567983, "stop": 568132, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACAAAAATTTAATAGAATATTTTTTATAGTTACAGATGGTCTTGGCATTGGATATGAACCACGCCAAGCTGAATTTCATGATGAAGGTGCAAATAGCATGTTGCATGCATCAGAAGCATTGCCACTAGAAATTCCTACATGA", "aa": "MQEQKFNRIFFIVTDGLGIGYEPRQAEFHDEGANSMLHASEALPLEIPT", "aa_hexdigest": "4eec1535153f3fde8ad1d1f95641cfd4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.166199999999, "isoelectric_point": 4.583291816711426 }, "id": "LMJBGEANGM_981", "locus": "GCA013368015_00981" }, { "type": "cds", "sequence": "contig_1", "start": 568424, "stop": 569674, "strand": "+", "frame": 2, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_L0RUB4", "UniRef:UniRef90_D1J8K6" ], "nt": "ATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGCGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACATTCCAATCTCATTAATGCTTGATACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAAAACGGATGAAAATTCATACAAGAATTTTTTGGGTACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAAAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAGGATGTTAAAAAATTCGAAATTATTGTTGAAACAGTTAATGAACATTTCTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAGGATATTGACGATATTATATGGGGTGCAAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAATGCAAATGGCGGAGAAAACATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATTAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTAGCAAGAGGGGACCTTGGACTTGAAATTCCTTACTATGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTCGGCAAGGAAGTTATAGTTGCAACTCAAATGTTAGATTCAATGGAAAACAACCCTATTCCAACAAGAGCGGAAGTTACTGACGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCTGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTGGCAAATCATCCTACTTCAAGAATGTTAATTGCTTATGAAGTTGCAAAAATCCTTCAAAGCGGTGAATACAAATTTGCTATTATATTAAGTCACACCGGAAAATTATTAAAAGAAGTTGCTAAATACAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTGGTTTGA", "aa": "MKFNELRTKIVATIGPSSDNYDTMKSLIEAGVTTIRANFSHGGPETHVNKFEIARQISKELNIPISLMLDTKGPEIRVGKMENDGVTIPANHILKIKTDENSYKNFLGTQDQITVSYRMDNDLKIGDKVLIDDGKLVTTVKDVKKFEIIVETVNEHFLKTNKRINIPGVNFSLPFLSQKDIDDIIWGAKYKVDYIAASFVNSAKNVKEIRDILNANGGENIQIISKIESKMAINNIDEIIEASDGIMVARGDLGLEIPYYDVPYYEKKIIRKCRLVGKEVIVATQMLDSMENNPIPTRAEVTDVYYAVELGADSTMLSGESASGKYPVEAVKVMSKIAKRAEKEYYNELFYGKQIEKVANHPTSRMLIAYEVAKILQSGEYKFAIILSHTGKLLKEVAKYRPNAFIVGVVSDNRLV", "aa_hexdigest": "bcbe4b59702b4bb9be268bf04bb2ce4b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8K6", "query_cov": 1.0, "subject_cov": 0.879492600422833, "identity": 0.998, "score": 808.0, "evalue": 1.47e-294, "valid": true, "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_L0RUB4", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUB4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUB4" ], "product": "Pyruvate kinase" }, "genes": [ "pyk" ], "id": "LMJBGEANGM_982", "locus": "GCA013368015_00982" }, { "type": "cds", "sequence": "contig_1", "start": 569999, "stop": 570127, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTTCAGGCTTAGCTATTGATAGAGTTGTTGCTGCTATTTTAGAAAATTATCAAAACCCAGATGGTTCAATCACAATTCCAAAAGTTTTAGTTCCATACATGGGTAAAGAAGTTATTAAATAA", "aa": "MNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK", "aa_hexdigest": "daddee63f10a648e665742e1012dd38b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M4S1T5", "query_cov": 0.8809523809523809, "subject_cov": 0.8043478260869565, "identity": 0.649, "score": 54.7, "evalue": 3.78e-09, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_M4S1T5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4515.254900000001, "isoelectric_point": 5.97095890045166 }, "id": "LMJBGEANGM_983", "locus": "GCA013368015_00983" }, { "type": "cds", "sequence": "contig_1", "start": 570248, "stop": 570364, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATGATGCCAAAAGCATTCTTGAATTACTTGAAATTCCATACCGTGAAATGATGCTTTCAACTGGCGATATTGGATTTAGCTCAGCAAAAACTATTGACCTAGAACTATGA", "aa": "MVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLEL", "aa_hexdigest": "57b07484f1a654554c468177557b5823", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4244.898999999999, "isoelectric_point": 4.196388816833497 }, "id": "LMJBGEANGM_984", "locus": "GCA013368015_00984" }, { "type": "cds", "sequence": "contig_1", "start": 570371, "stop": 570526, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGTCTAAACCAAAGAAATTTGTTGCATATACAAAATGTTTCCGTTCAGAAGCAGGAAGCAGTGGAAAAGATACAAAAGGTTTGATTAGACAACACGAATTCCATAAAGTCGAACTTGTAAAAATAACCAGTGAAAAAGATGCAATGAAAGAATGA", "aa": "MSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKE", "aa_hexdigest": "1bf5c3511f53ff80841e97b1b2e2591e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 46, "aa_cov": 0.9019607843137255, "hmm_cov": 0.9130434782608695, "evalue": 4.423683269786109e-11, "score": 38.68495178222656, "start": 4, "stop": 49 } ], "seq_stats": { "molecular_weight": 5830.716199999999, "isoelectric_point": 9.61141414642334 }, "id": "LMJBGEANGM_985", "locus": "GCA013368015_00985" }, { "type": "cds", "sequence": "contig_1", "start": 570584, "stop": 571264, "strand": "-", "frame": 2, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q98RH5", "UniRef:UniRef90_D1J8K7" ], "nt": "ATGTTAGATTTAAAATATATTATTAGTAACAAAAATGAAGTTATTAAAAAACTATCAACACGTAATTTCAATATTGCTATTATCGAAGAAATTTTTGAAGACGCTCAAAAAAGAAACGAATTAATTCATAATTTAGAAACTTTGCAAGCCAAAAGAAATAAATTAAGTGAACAAATTGGAATTAATAAAAGAAACAAGCAAGATTGCTCAGAATTATTACAACAAGTTGCTGAAGTTAAAGAACAAATTGAAAAAATTGATGAAGAAGCTGACACATTGGTAAAGAAAGTCAACACATTGCTATACCAAATTCCTAACTTGCCATATGATGAAGTTCCTGTAGGAAGAGACGAAGATGACAACGTAGTTTTAAAAGAACATCCAAATTTAGGAAGAGGCTTAGTAAAAGGCGTATTGCCACATTATGAAATAGCAACAAAATTAGATATTGTTGATTTTGTTAGATCAGTGAAGTTAGCAAGCACAAGATTTGTTTTATACAAAAATGATGGTGCTAAATTAGTAAGATCCTTAGCCTCTTTTATGCTTGATACGCACATTGCAAATGGATATAAAGAATTAATGCCTGCCCATTTAGTTAATTCAAATATGCTTTATGGAACGGGACAATTGCCTAAATTTGCAGAAGATTTATTTAAAATGCAAGGCAATGACCTTTGA", "aa": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDL", "aa_hexdigest": "799214d340c25b0e645f110351528f7b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "serS" ], "truncated": "5-prime", "pseudogene": { "start": 570281, "stop": 571264, "observations": { "mutation": [ 570373, 570586 ], "stop": [ 570373, 570586 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K7", "query_cov": 1.0, "subject_cov": 0.5368171021377672, "identity": 1.0, "score": 376.0, "evalue": 8.29e-128, "gene_start": 1, "gene_end": 226, "reference_start": 1, "reference_end": 226, "reference_sequence": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDLWLIPTAEVPVTNYYYDEILDLSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKEWESMVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLELWIPSEQRYRETSSISICRDFQGRRAKIRYRDETGHTKYAYTMNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK" }, "paralog": false, "identity": 0.9938837920489296, "score": 575.0, "evalue": 7.95e-209, "description": "Internal stop codon at 570373, 570586. Nonsense mutation around 570373, 570586.", "psc": { "uniref90_id": "UniRef90_D1J8K7", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q98RH5", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K7", "EC:6.1.1.11", "UniRef:UniRef50_Q98RH5", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RH5" ], "product": "Serine--tRNA ligase" } }, "id": "LMJBGEANGM_986", "locus": "GCA013368015_00986" }, { "type": "cds", "sequence": "contig_1", "start": 571539, "stop": 571892, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTGTTAAAGTTAATAAATACAATAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACCTTAAACAGCCACATTTATGAAATATCAGTTTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAACTATGTTATTAAATTTTTAAAAAGTAGTCATTTTCCTGGTATAGGCGAAGAAAAAGCAAAATTAATTGTAAAAGATTTAGGGATCGATGCTTTAAGAAAAATGGTTGAGGATAATTCAATTGATTATTCAAAATATTCAATATCAGAATTGGCATGA", "aa": "MNPVKVNKYNSISVTLKNNLFSEKNITLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELA", "aa_hexdigest": "8945624e5fd1ae3649eb132377623800", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13389.057699999987, "isoelectric_point": 6.111578559875488 }, "id": "LMJBGEANGM_987", "locus": "GCA013368015_00987" }, { "type": "cds", "sequence": "contig_1", "start": 571944, "stop": 572255, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTTAACAAATAATTTAACGCCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTATAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGTATCTCACTTGAAGATATGGACAAATTACATAATATCTTTAACAAATTTGAACACCCCTTTAAACCTGCAACCCACTTGTACAAATCATTTTTAAAATATCTATATAATTCTGGAAACACTAGAATTTTAAAAGATTATTTATACGACCAGCTAATAAACTTTGCAAGCCAAGACACTGAATGA", "aa": "MFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTE", "aa_hexdigest": "bbd10f8aa144e225834635c5cbecca17", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12574.095599999982, "isoelectric_point": 5.754743385314941 }, "id": "LMJBGEANGM_988", "locus": "GCA013368015_00988" }, { "type": "cds", "sequence": "contig_1", "start": 572295, "stop": 573677, "strand": "+", "frame": 3, "gene": "recD", "product": "Exodeoxyribonuclease V alpha chain", "db_xrefs": [ "COG:COG0507", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J8K8", "UniRef:UniRef90_D1J8K8" ], "nt": "TTGGTTCAAAATAAATATTTAATCGAAATTGAATACGAAGATGGTACATATCTTAGTGCCAAAGACGTATTTGATATGGAGAAATATATTGTCAAAAGATTGAAACATATTGAATCTGCTTCAAACCCAAAATTCATTAATTTCAAGCCAAGTGAAATGTTCCACCCTTTGCAACTTCAAGCAATTGAAACAGCATTAACTAGCAAATTGACATTGATTACAGGAAACCCCGGCACTGGAAAAACATTAATAACTAATGAAATAATTAAACAACTGCTTTCGGTCTATGATGAAGATGATTTGGCAATAGTTACACCAACAGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAAACAAATGCAGTTACTATACACAGTTTTTTAGAATGGGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTGGATTTATTTTATTCACTACTTAAAGGAATAAAAAAGAATTCATTAAAACGAATCATATTAGTTGGCGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGATGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCTCAGTTTATCGAAACAAAACAGAGTGATCTAAAGTCAATGTTACTCTTTAAATTAAATGACTTATTGCAACAAGGCTATAGCAAACGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGATCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAATATAGAGACCATATTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCTTTTGAAAAAATTTTGGCAAGTATCTCTGTAGATTTTGAAAACGATTCAAAAACTGTTTCTTATTCTAGAAAAGATTTTTTTGCTAATACATATCCTGCATATTGCACAAGCGTTCATAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCAAAAAAACTTATTTATACCGCTATTACTAGAGCAAAAAAATATTGCGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAATGAATAA", "aa": "MVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESASNPKFINFKPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHILAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKILASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE", "aa_hexdigest": "86c9a27b33a3f13327371de308007b4f", "start_type": "TTG", "rbs_motif": null, "genes": [ "recD" ], "truncated": "5-prime", "pseudogene": { "start": 571995, "stop": 573677, "observations": { "mutation": [ 572253 ], "stop": [ 572253 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K8", "query_cov": 1.0, "subject_cov": 0.6157965194109772, "identity": 0.991, "score": 899.0, "evalue": 0.0, "gene_start": 1, "gene_end": 460, "reference_start": 288, "reference_end": 747, "reference_sequence": "MLQNSNIQGKFNKIMWTSTDGQVVIVSFLVSSNDGLNPVKVNKYNSISVTLKNNLFSEKNVTLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELTWNYAVEFIKNNPTLIEDQMFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTEWPRDVETFNNSVELLVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESSSNPKFINFMPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHILAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE" }, "paralog": false, "identity": 0.9928571428571429, "score": 1111.0, "evalue": 0.0, "description": "Internal stop codon at 572253. Nonsense mutation around 572253.", "psc": { "uniref90_id": "UniRef90_D1J8K8", "gene": "RecD", "product": "Exodeoxyribonuclease V alpha chain", "uniref50_id": "UniRef50_D1J8K8", "cog_id": "COG0507", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K8", "UniRef:UniRef50_D1J8K8", "COG:COG0507", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8K8" ], "product": "Exodeoxyribonuclease V alpha chain" } }, "id": "LMJBGEANGM_989", "locus": "GCA013368015_00989" }, { "type": "cds", "sequence": "contig_1", "start": 573677, "stop": 574237, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_012855695.1", "SO:0001217", "UniParc:UPI0001BC2435", "UniRef:UniRef100_D1J8K9", "UniRef:UniRef50_Q98PE2", "UniRef:UniRef90_D1J8K9" ], "nt": "ATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCACAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATTGACAAAGAAGTTATTTTAGCCAAACCGTTAACATTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTGCCAGTTGGAAAAGCTGTTATTAGACAAAAAGGTTCGTCTGGTGGACATAATGGAATGAAAGATATAATTGCTAAATTAAAAACTGATGAAATAAAAAGATTAAAAATAGGAATTGGACGTGGTCAGAATGTGATTAATTATGTTCTAGGTAAATTTAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGATATTGCTTCTGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAA", "aa": "MKLIVGLGNPGVEYEKTRHNTGFMVIDKLAEKLHTPLTEKKFNGIYYIDKEVILAKPLTFMNNSGDFVRAIKDYYNIVDDDIIIVYDDLDLPVGKAVIRQKGSSGGHNGMKDIIAKLKTDEIKRLKIGIGRGQNVINYVLGKFSYQDFCTIEKIIDIASDALISFISNDIRFVMNKFSGELYRENK", "aa_hexdigest": "ffa6914d0b626ec4bf14b6ed375ee7a8", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC2435", "ncbi_nrp_id": "WP_012855695.1", "uniref100_id": "UniRef100_D1J8K9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2435", "RefSeq:WP_012855695.1", "UniRef:UniRef100_D1J8K9" ] }, "ips": { "uniref100_id": "UniRef100_D1J8K9", "uniref90_id": "UniRef90_D1J8K9" }, "psc": { "uniref90_id": "UniRef90_D1J8K9", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q98PE2", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PE2" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "LMJBGEANGM_990", "locus": "GCA013368015_00990" }, { "type": "cds", "sequence": "contig_1", "start": 574224, "stop": 574658, "strand": "+", "frame": 3, "gene": "ttcA", "product": "tRNA(Ile)-lysidine synthase", "db_xrefs": [ "COG:COG0037", "COG:J", "EC:6.3.4.19", "KEGG:K04075", "SO:0001217", "UniRef:UniRef50_D1J8L0", "UniRef:UniRef90_D1J8L0" ], "nt": "GTGAAAACAAATAATTTAGTTAAATTACAACGTGACTTATTAAATGAATTTAATAAATGCAATATTGACGTCAATAAAAAATTTCTAATTGGGGTAAGTGGTGGCCCTGATTCAATGTGGCTACTTAATTTACTTAAATCATTGAATATTGTTGCTGCGCATGTAAACTACAACAAAAGATATGATAGCAATTATGATGAATCATTGGTTTCACGATTTTGTGCTAATAACAATATCCCTTTAGAAATACTTTCATTACATAACAAATATTCTAGTGGTAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAATTTCTAAAAAATATCAATGCGACTATATCCTCCTTGCTCACCAAAAAGATGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCCTTATTTTGA", "aa": "MKTNNLVKLQRDLLNEFNKCNIDVNKKFLIGVSGGPDSMWLLNLLKSLNIVAAHVNYNKRYDSNYDESLVSRFCANNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEISKKYQCDYILLAHQKDDFLESSIMQENSNRKPLF", "aa_hexdigest": "de3255ce56e8e16305de423fab1d0fc1", "start_type": "GTG", "rbs_motif": null, "genes": [ "ttcA" ], "truncated": "3-prime", "pseudogene": { "start": 574224, "stop": 574961, "observations": { "mutation": [ 574656, 574719, 574935 ], "stop": [ 574656, 574719, 574935 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L0", "query_cov": 1.0, "subject_cov": 0.4630225080385852, "identity": 0.965, "score": 283.0, "evalue": 3.2e-94, "gene_start": 1, "gene_end": 144, "reference_start": 1, "reference_end": 144, "reference_sequence": "MKTNNLVELQRDLLNEFNKCNIDTNKKFLIGVSGGPDSMWLLNLLKSLNIVVAHVNYNKRYDSNYDESLVSQFCADNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEISKKYQCDYILLAHQKDDFLESSIMQENSNRKPLFWGIKQLSVLNNNIIFRPMLNLWTKQEIIDFCNEYSILFATDYTNAETHYTRNKIRSNLKKLDPEQKQNKYQFFVNKNIENEHIEKQVLESFEDWKNNQFNVDKLDKNFVYIDQLLFYTLNHFYNNLKLSLNKIHGIKDFLFSQNRTKNFKLNDEQYIHKIKNLLIFK" }, "paralog": false, "identity": 0.9551020408163265, "score": 477.0, "evalue": 5.55e-173, "description": "Internal stop codon at 574656, 574719, 574935. Nonsense mutation around 574656, 574719, 574935.", "psc": { "uniref90_id": "UniRef90_D1J8L0", "gene": "TtcA", "product": "tRNA(Ile)-lysidine synthase", "ec_ids": [ "6.3.4.19" ], "uniref50_id": "UniRef50_D1J8L0", "cog_id": "COG0037", "cog_category": "J", "kegg_orthology_id": "K04075", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L0", "EC:6.3.4.19", "UniRef:UniRef50_D1J8L0", "COG:COG0037", "COG:J", "KEGG:K04075" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8L0" ], "product": "tRNA(Ile)-lysidine synthase" } }, "id": "LMJBGEANGM_991", "locus": "GCA013368015_00991" }, { "type": "cds", "sequence": "contig_1", "start": 575207, "stop": 575416, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATCCAAAAGAAAAAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTTATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAATGTTATTAGAGGAACTGCTAACAAAAATGATGGAAACTATATTTCTTCATGA", "aa": "MNNPKEKKPRKIGLFGIIGILFAIIAVITLGYIISDVVKERMSNFQTLNFLENVIRGTANKNDGNYISS", "aa_hexdigest": "7b75372bc2f7ddd2b075f58d089ff3ee", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7699.945, "isoelectric_point": 9.77445468902588 }, "id": "LMJBGEANGM_992", "locus": "GCA013368015_00992" }, { "type": "cds", "sequence": "contig_1", "start": 575615, "stop": 576916, "strand": "+", "frame": 2, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "nt": "TTGAATTCATGTGCAACATTTAATGGTCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAGCCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTTGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGTAAGGGAAGAGGAATGCTAACTGGCGGTATTGATACAAATAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCTGGCGCTAGAATTCCAAAAGGAATATTACTAGGAGGCCCTCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAACGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTGGGTGCAAAAAGAGTTCGTGACATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGATGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGATGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAGATGGATGGTATCAAAGAAAATAGCGGTATTTTAATAATGGCTGCTACAAATAGAACCGATGTTCTAGACCCTGCTTTATTAAGACCAGGACGTTTTGATAGAACCATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATCTTAAAATTACATGCTAATGGAAAAAGAATTGCAAACAATATTAATTTTGCTATGGTGGCAAGAAGAACTCCTGGTTTCTCTGGAGCACAACTTGAAAACGTAATTAACGAAGCCTCTTTATTAAGTGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACCATTTCTGAAAAAGAAAACATTGCAGTTGCCTACCATGAAGCGGGACACGCAGTTGTTGGAATAAAAATTAAAGGTGGAAATAAAGTTCAAAAAATAACCATCATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCTGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCGTTTATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGACGATATTGCTAAGGCAACAAATATTGCTAGAAAAATGGTTACTGAATGA", "aa": "MNSCATFNGQVLGNIVASSMPKPSIASKYIIPAFSAFVGLAFIIIIMAMYFGKGRGMLTGGIDTNKSLAQRIYSNKKFSDIAGNEEVKEEVKELVDYLKNPKKYEAAGARIPKGILLGGPPGTGKTLIAKATAGEANVPFFFISASNFVEMFVGLGAKRVRDMFAEARSHAPAIIFIDELDAVGRSRGAGIGGGNDEREQTLNQLLVEMDGIKENSGILIMAATNRTDVLDPALLRPGRFDRTITVGLPDIKEREAILKLHANGKRIANNINFAMVARRTPGFSGAQLENVINEASLLSVRENTGVITIEQIDEAIDRVIAGPAKKSRTISEKENIAVAYHEAGHAVVGIKIKGGNKVQKITIIPRGKAGGYNLMMPDEEKYNRSKSELLAIITSFMGGRVAEAIIYGADNVSTGASDDIAKATNIARKMVTE", "aa_hexdigest": "cbe20e98ba28c4bcbee3448936312e36", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A485JC26", "query_cov": 0.9468822170900693, "subject_cov": 0.8102766798418972, "identity": 0.512, "score": 403.0, "evalue": 5.68e-134, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "ec_ids": [ "3.4.24.-" ], "uniref50_id": "UniRef50_A0A485JC26", "go_ids": [ "GO:0004176", "GO:0004222", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_A0A485JC26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "LMJBGEANGM_993", "locus": "GCA013368015_00993" }, { "type": "cds", "sequence": "contig_1", "start": 576986, "stop": 577426, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01434.24" ], "nt": "ATGAAAAATTCTTCATTTTCAAGTAAAGTAGGTCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACTGCTGAAAAGAAGGCTCATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTTGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTACCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAATGATTCAAGCAACCATTCAGAAGATTCTAATAATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAA", "aa": "MKNSSFSSKVGQEIDEQIRKIILTAEKKAHEIITENRQLLELIKDALIIKETIVAEEIEYIEKHMELPPEITNTKKELKNEYSEEDFNELFNEVAGQKQIQEDKYKATLNSELKKHNIEDNDSSNHSEDSNNTKNSDEDSNKEDNQ", "aa_hexdigest": "b9ced80b2258dd92d3c4d20de8833d60", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01434.24", "name": "Peptidase_M41", "length": 59, "aa_cov": 0.4041095890410959, "hmm_cov": 1.0, "evalue": 8.112791536514134e-11, "score": 37.650604248046875, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 16980.43399999999, "isoelectric_point": 4.682134819030761 }, "id": "LMJBGEANGM_994", "locus": "GCA013368015_00994" }, { "type": "cds", "sequence": "contig_1", "start": 577452, "stop": 577805, "strand": "-", "frame": 1, "gene": "ygaC", "product": "DUF402 domain-containing protein", "db_xrefs": [ "COG:COG3557", "COG:S", "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_A0AA43TZF8", "UniRef:UniRef90_D1J8L2" ], "nt": "TTGTTTTTCTTTGCTAGAAACAAATTTTATAATGCTTCTATTACATTAAATAAATCTAAAGAAAACTATATTTATATTAATTTAGCAAGCCCTTTTTATATTGAAAATGAAGTAATTAAATATATTGATTTTGATATCGATATTAAAAGTTATGATGATAAAGATTTTAGCGTTATAGATTGGCAAGATTTTAAAAATTCAATTAGTCAATACAATTATTCATTGAAATTAATTTATAAATTGTATGAAGAATTAGATTTTTTATATGAACAAAAGCAAAAAGGAACAGGAGTTTTTTCTAAAAAACTTGTTAACGGCCTTGAAAAGATGTTGCGAGAAAGCGGAGATATATAA", "aa": "MFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI", "aa_hexdigest": "3b42402dbf2f9f440d25f246ec1fd3a3", "start_type": "TTG", "rbs_motif": null, "genes": [ "ygaC" ], "truncated": "3-prime", "pseudogene": { "start": 577452, "stop": 578066, "observations": { "mutation": [ 577826 ], "stop": [ 577826 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L2", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.991, "score": 228.0, "evalue": 2.52e-74, "gene_start": 1, "gene_end": 117, "reference_start": 88, "reference_end": 204, "reference_sequence": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLVKTKVSEKSINWVVSEPILFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI" }, "paralog": false, "identity": 0.9901960784313726, "score": 401.0, "evalue": 4.01e-145, "description": "Internal stop codon at 577826. Nonsense mutation around 577826.", "psc": { "uniref90_id": "UniRef90_D1J8L2", "gene": "YgaC", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_A0AA43TZF8", "cog_id": "COG3557", "cog_category": "S", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L2", "UniRef:UniRef50_A0AA43TZF8", "COG:COG3557", "COG:S", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA43TZF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43TZF8" ], "product": "DUF402 domain-containing protein" } }, "id": "LMJBGEANGM_995", "locus": "GCA013368015_00995" }, { "type": "cds", "sequence": "contig_1", "start": 577824, "stop": 578066, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGAAAGTATAAAGAGTTTTGATGATTTATTAAAAACAATTGATACACCAAACTCAAAAAATATAAAAAAACATAAAAAGTTTTTTAAGATGGGACAACTAACAACCATTCAAGTTTTTAAATATGATGGAACTTTATACAGACAATATGAAGGGGCAAAAATAGTTGCAAATTTAACTGACTTTGTAGTTGTTTTGTTAATGAAAACAAAAGTTTCTGAAAAATCTATCAATTGA", "aa": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLMKTKVSEKSIN", "aa_hexdigest": "94f70607d96c17d2aa44eef453fc226f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9277.7353, "isoelectric_point": 9.363597679138184 }, "id": "LMJBGEANGM_996", "locus": "GCA013368015_00996" }, { "type": "cds", "sequence": "contig_1", "start": 578320, "stop": 578586, "strand": "+", "frame": 1, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_Q9F411", "UniRef:UniRef90_Q9F411" ], "nt": "ATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAAAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAATGCACCCTGACATAGAATATATCGGACTTGAAAAATACAGCACGCCCGCCTATTCTGCTTTAAAAAAAGCAATTGACCTCAACCTTGAAAATTTTCACATAATTATAGGAGATGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGA", "aa": "MASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALMHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTI", "aa_hexdigest": "b82d40745df411e9eea4445fba71bb60", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 578281, "stop": 578889, "observations": { "mutation": [ 578584, 578605 ], "stop": [ 578584, 578605 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9F411", "query_cov": 1.0, "subject_cov": 0.43564356435643564, "identity": 1.0, "score": 179.0, "evalue": 1.47e-55, "gene_start": 1, "gene_end": 88, "reference_start": 14, "reference_end": 101, "reference_sequence": "MRLRFNKNAETSLMASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALMHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTIWLTFSDPWPKKRHYKRRLVYRDFLKIYQNVLDKDGVVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFKIENVFTDYEEKFNKLNKNINFIAFTFN" }, "paralog": false, "identity": 0.9801980198019802, "score": 402.0, "evalue": 6.06e-146, "description": "Internal stop codon at 578584, 578605. Nonsense mutation around 578584, 578605.", "psc": { "uniref90_id": "UniRef90_Q9F411", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_Q9F411", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9F411", "EC:2.1.1.33", "UniRef:UniRef50_Q9F411", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_Q9F411", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9F411" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "LMJBGEANGM_997", "locus": "GCA013368015_00997" }, { "type": "cds", "sequence": "contig_1", "start": 578689, "stop": 578889, "strand": "+", "frame": 1, "gene": null, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PL40" ], "nt": "GTGGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATCAATGCAAAAATCATTTATCAAACTTCTGATTTACACCATTGCAATTTTGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCGTTTACTTTTAATTAA", "aa": "MVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFEIENVFTDYEEKFNKLNKNINFIAFTFN", "aa_hexdigest": "36e27a7026d4feab4277b0b28476646a", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PL40", "query_cov": 0.9848484848484849, "subject_cov": 0.8441558441558441, "identity": 0.615, "score": 93.6, "evalue": 8.69e-24, "valid": false, "product": "tRNA (Guanine-N(7)-)-methyltransferase", "uniref50_id": "UniRef50_A0A3B0PL40" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PL40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PL40" ], "product": "tRNA (Guanine-N(7)-)-methyltransferase" }, "genes": [], "id": "LMJBGEANGM_998", "locus": "GCA013368015_00998" }, { "type": "cds", "sequence": "contig_1", "start": 578964, "stop": 580637, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_D1J8L4" ], "nt": "ATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAATAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGACGAAGAGGACGAAGAAGAAATCCCTCAAGACAAAGAAGGCTATAAGGTAGAACCCCAACTTTTAGATAAAGAAATTAATGGTTTAAGACCCGCTAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCACGTGCTCTACCTGATGCTCGTGATGGTCTAAAGCCAGTACACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCAGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCTTTAATTGATGGACACGGTAACTTTGGATCTGTTGACGGAGACGAAGCTGCTGCAATGCGTTATACAGAAGCTAGAATGAGCAAGATTGCTGGCGCAATGGTTGATGGTATTAAGAAAAATACTGTTGACTTTATGGACAACTATGACGCAACTGAAAAAGAACCAGTTGTTTTACCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTAGGTGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTAATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATTATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCAAACAGAGATGGCATTAGAATTGTGATTGATATCAAAAAATCTTTTGTTCCCGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGCTGGTAAATAATGAACCAAAATTGTTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGATTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTCTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCAAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTAAGTGAAATACAAACAAAAGCAATTGTTGATATGAAGCTAGGAAAATTAACTGGATTAGCAATTGAAAAAATGAACGAAGAACTAAATGAAGTACATGAAAGAATTGCTAACTACATTAAGATATTAGGCGACCATAACTTATTAATCAATTTGATAATTGAAGAATTACAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGA", "aa": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEEIPQDKEGYKVEPQLLDKEINGLRPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGKLTGLAIEKMNEELNEVHERIANYIKILGDHNLLINLIIEELQEIKNAYGDARRSDIN", "aa_hexdigest": "2db74e3d21ae570866002879235bb032", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0015AA695A", "query_cov": 0.8725314183123878, "subject_cov": 0.8983364140480592, "identity": 0.591, "score": 573.0, "evalue": 3.08e-198, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2N1QA61" }, "genes": [ "gyrA" ], "truncated": "3-prime", "pseudogene": { "start": 578964, "stop": 580940, "observations": { "mutation": [ 580635 ], "stop": [ 580635 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L4", "query_cov": 1.0053859964093357, "subject_cov": 0.612691466083151, "identity": 0.982, "score": 1049.0, "evalue": 0.0, "gene_start": 1, "gene_end": 557, "reference_start": 1, "reference_end": 560, "reference_sequence": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEDEEEIPQDKEGYKVEPQLLDKEINGLKPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIRILGDHNLLINLIIEELQEIKNAYGDARRSDINWDELSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKLDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE" }, "paralog": false, "identity": 0.983358547655068, "score": 1269.0, "evalue": 0.0, "description": "Internal stop codon at 580635. Nonsense mutation around 580635.", "psc": { "uniref90_id": "UniRef90_D1J8L4", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L4", "EC:5.6.2.2", "UniRef:UniRef50_B3PN30", "COG:COG0188", "COG:L", "KEGG:K02469", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" } }, "id": "LMJBGEANGM_999", "locus": "GCA013368015_00999" }, { "type": "cds", "sequence": "contig_1", "start": 580644, "stop": 581699, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "TTGAGCAGCATTGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACATTAACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTTGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTAATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGCCACGAAGTTCCCGTTGGCACCAAACAATCAAAGGGTATTCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAAATTTTGCCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACAATTTTATTTGAAAGTATTAACAGAAATGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTTAAAGACGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGGTCAATGGGAAGAATGGCCTATGGTGTTAAGGGAATTAAAATTGACGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTTGGAAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACTAGACGTGGAGCAAGTGGAGTTTATTCATTAAATGAAGAAAAATCTGGTAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTAATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAACATTGTTGCAGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCCAAAAATAACCTTAATCAAAAAAATGAATAA", "aa": "MSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKIDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTNIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE", "aa_hexdigest": "cf2138383e6552fe7ce5a56f5252486d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 48, "aa_cov": 0.13675213675213677, "hmm_cov": 1.0, "evalue": 3.0265936586223565e-57, "score": 185.42904663085938, "start": 14, "stop": 61 } ], "seq_stats": { "molecular_weight": 39174.226100000014, "isoelectric_point": 8.587204933166507 }, "id": "LMJBGEANGM_1000", "locus": "GCA013368015_01000" }, { "type": "cds", "sequence": "contig_1", "start": 582073, "stop": 582174, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAGAATTGATCCAAATAAATCAATATACAAAGAAGTTAATGATACTATAAAACGCTTAGAACAAAGTGGAAAATATGCCTCAAAAATTTCTGAATGA", "aa": "MTRIDPNKSIYKEVNDTIKRLEQSGKYASKISE", "aa_hexdigest": "fa61966352c9a8058a6181d12f21f3dc", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3843.3222000000005, "isoelectric_point": 9.223185539245605 }, "id": "LMJBGEANGM_1001", "locus": "GCA013368015_01001" }, { "type": "cds", "sequence": "contig_1", "start": 582541, "stop": 582789, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAATTTAATAAGCTAGATAGTAGTGTAAAAGATCCAGATTTTTTAGGTGTAACACAACAACTAAATGAAATAAAACAAAAGGCATTGCAAGAATTAACAAATATTAAAAATAATTCTAGTCAATTGGCAACTATTGCAGAAGCGTCAAAATATGAAAATGATATACAAGAAGTACAAGAAAAATTTGTTAAAGATTTAATAAAAATATTTGACGGAAATATAAACACAGATAGGATTAATTGA", "aa": "MQKFNKLDSSVKDPDFLGVTQQLNEIKQKALQELTNIKNNSSQLATIAEASKYENDIQEVQEKFVKDLIKIFDGNINTDRIN", "aa_hexdigest": "daa0fac88dcdcbaffcf6a9ea9395b0ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9400.46069999999, "isoelectric_point": 5.0127672195434565 }, "id": "LMJBGEANGM_1002", "locus": "GCA013368015_01002" }, { "type": "cds", "sequence": "contig_1", "start": 582925, "stop": 583857, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C31", "UniRef:UniRef90_UPI001F519C31", "PFAM:PF04778.17" ], "nt": "ATGAACAATAAAAGCAAAAAGATCGTTCTTCCATTAGCAATTTTTTGTGGCGGATTATCAATAGCTGCCATCGCTACCGTAGCAATAAGATCGTGTCAAAATAAAGTGGATAAAAAGACACAAAAAGAATCACTATTTAAAAAATTTAAAATTTTTCAAGAAGAATTGCAATTATTGCTAGAAAATAAAAACATTCAAGGAATTTATTCAATTGAAGAACAAAATGTCTTTGATAATTCCAAAATGATCAATATTGAATCGCCAATTGATAATATTAATCAAGAAATTTTTAAGATTAAAAGTTCGATTGATTCATTAAATGCAAAAATTCAAAAGCAAAGCATGCAAAAACTACTTTTTGCAGCAAAGAAACAGTTGGAAAATATTATTCATGCTATGAGGATATTGGAAAATAAATTAATTGATAATGATGATGATATTCAAAAAACTAAGGAAGATTTAAATTCACAAATTATTAATGCATTAGAGGTTTATAACAAAAATAATATAGACGAAATAAATAATTTAAATAAATCATTAACTTTTAAAATTTCTGAAGTTGAAGATATATTGATCAAATTTAAAAAATCAAAAGAACAAGAATTTGAAAAATTACAAAAAACTAGAAAATTAGTTGAAGAATTTTTGGCCAAAGATGTTGTTAAATCTGATTATTATGCAAATATAGTTAGAAAGCTTAATAGAGAAGTTTCAAGACGAGGAAACGTTTCAATTAGTTCTAATAAATCAGAAATAGTAGCTACAAATATTCATTTGAACGAAATTTTAAGGCAAAGTAAAGAATTCGTTGAAAATGATATGCAAAAATTAAGGGACAGATTAATTTCTTTAATTATAGAAGCAAAAGAATATATTGAAAATAAAATGCGCCCTAGAGATAAATATAACGCATACATTGAAAGAATAGAATGA", "aa": "MNNKSKKIVLPLAIFCGGLSIAAIATVAIRSCQNKVDKKTQKESLFKKFKIFQEELQLLLENKNIQGIYSIEEQNVFDNSKMINIESPIDNINQEIFKIKSSIDSLNAKIQKQSMQKLLFAAKKQLENIIHAMRILENKLIDNDDDIQKTKEDLNSQIINALEVYNKNNIDEINNLNKSLTFKISEVEDILIKFKKSKEQEFEKLQKTRKLVEEFLAKDVVKSDYYANIVRKLNREVSRRGNVSISSNKSEIVATNIHLNEILRQSKEFVENDMQKLRDRLISLIIEAKEYIENKMRPRDKYNAYIERIE", "aa_hexdigest": "d0130b0027abbcffb15251853fdff7d7", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F519C31", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.929, "score": 518.0, "evalue": 2.59e-184, "valid": true, "uniref50_id": "UniRef50_UPI001F519C31" }, "pscc": { "uniref50_id": "UniRef50_UPI001F519C31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C31" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 154, "aa_cov": 0.4870967741935484, "hmm_cov": 1.0, "evalue": 1.8847003885925107e-53, "score": 176.161376953125, "start": 135, "stop": 285 } ], "seq_stats": { "molecular_weight": 36187.39890000004, "isoelectric_point": 9.140730476379396 }, "id": "LMJBGEANGM_1003", "locus": "GCA013368015_01003" }, { "type": "cds", "sequence": "contig_1", "start": 584263, "stop": 584472, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAAAACACAACCAATAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAGGTTATAGAAGATATTCAAATAAATGCCAATCCTACAATAGCAATAGATAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGA", "aa": "MKNTTNKMLNKSTTLLFTENFLFFINKVKKVIEDIQINANPTIAIDKTILIGINLVRFTAVSFIGKLLP", "aa_hexdigest": "f97a2cba848694ae3003f47691d11ce4", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7812.2802999999985, "isoelectric_point": 9.998095512390137 }, "id": "LMJBGEANGM_1004", "locus": "GCA013368015_01004" }, { "type": "cds", "sequence": "contig_1", "start": 584506, "stop": 584730, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAGCCACTTATTAAAGCAATAAAAACAGGCACTAACGCATATTCAATCATTCATGTACTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAATATACCTAAAAAGGCACCCTTTCACGGTCCTAATGCCAACCCAAAAATAATGAATACTGCATAA", "aa": "MLKKLNPIIHVIFCLAYINLNNQPLIKAIKTGTNAYSIIHVLIPLITWFSVSHNIPKKAPFHGPNANPKIMNTA", "aa_hexdigest": "286c2b3daeae1eff76a91d5d85a7d658", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8271.855099999993, "isoelectric_point": 10.111173057556147 }, "id": "LMJBGEANGM_1005", "locus": "GCA013368015_01005" }, { "type": "cds", "sequence": "contig_1", "start": 584688, "stop": 584891, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAATTTATTCAAAAAATTAGACGTATTAAAAAAACAAAACAACATAAATTAACAGTTTTTGAAATTTCTTTATTTGCTTTACTTTTAGCTATTTATATAATTGCTGCAATCTTAGAAAGATTTGTTTTCAAAGGAATAATGAATATTAATATTACTTATGCAGTATTCATTATTTTTGGGTTGGCATTAGGACCGTGA", "aa": "MFEFIQKIRRIKKTKQHKLTVFEISLFALLLAIYIIAAILERFVFKGIMNINITYAVFIIFGLALGP", "aa_hexdigest": "8d8277910469a4365480cd7504f96ef4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.507999999994, "isoelectric_point": 10.21090564727783 }, "id": "LMJBGEANGM_1006", "locus": "GCA013368015_01006" }, { "type": "cds", "sequence": "contig_1", "start": 585066, "stop": 585293, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTTTTGACTCGGACTATGAAGAATTTATGAAGGGTTCAATTGTTGCAGATTTGAAGAATACAGACTATACCGGACATGCAGGTTCTTGTTCAGCAGCAATGTTCTTAAAAGAATTTACAAAAGGAATTGAATATATTCACTTAGATGTTGCAGGAACTAACGATATTGATGAAAAACCAATGTTTGCAATGACAAAAACTTTGACAGAATTAGTGTTGAAATAA", "aa": "MPFDSDYEEFMKGSIVADLKNTDYTGHAGSCSAAMFLKEFTKGIEYIHLDVAGTNDIDEKPMFAMTKTLTELVLK", "aa_hexdigest": "d7f4f7412216ed7607c491ffc4bc2a8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 59, "aa_cov": 0.7866666666666666, "hmm_cov": 0.9491525423728814, "evalue": 8.314044981302672e-15, "score": 50.34300994873047, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8373.472299999996, "isoelectric_point": 4.60369701385498 }, "id": "LMJBGEANGM_1007", "locus": "GCA013368015_01007" }, { "type": "cds", "sequence": "contig_1", "start": 585363, "stop": 585980, "strand": "-", "frame": 1, "gene": null, "product": "M17 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "nt": "TTGGGTATTACCCCTCCTAATGAATTAAATTCTGAACAATTAGCTAAAATAGTTGTTGAAGACTTTAAAGAATATAAAAACTTATCAGTTAAAGTTCTTGAAAAGAAAGAAATTGAAAAATTGGGTATGGGATTATTACTATCTGTGAATAGAGGTTCAGTTTATGAACCTAGAGTTGTAATAATTGAATATAAAGGAAATCCTGATTCAAATCAAAAAACAGTTTTAATTGGTAAAGGAATTACCTTTGACTCTGGCGGTTACAACATTAAAACAGGCCGTAATATGTTAGGAATGAAATATGACATGTCAGGTTCTGCAATAGTTGCATCTGTTTTAAAATCTGTTGCTCAATTAAAACCAAAAACAAATGTATCAGCAATTATGTGTATTACTGACAATAGAGTTAATGGAGATGCATCATTACCTGACTCAGTTTGGACTTCAATGAGCGGAAAGACTGTTGAAATAAATAATACAGACGCTGAAGGTAGATTAGTTATGGCTGATGGTTTGTACTATGGAGCTGATGTATTAAAAGCAACAAGATTAATTGATGTTGCTACATTAACAGGCGCAATGGTTGCAGCTCTAGGAGACACATATACAGGCATTTGA", "aa": "MGITPPNELNSEQLAKIVVEDFKEYKNLSVKVLEKKEIEKLGMGLLLSVNRGSVYEPRVVIIEYKGNPDSNQKTVLIGKGITFDSGGYNIKTGRNMLGMKYDMSGSAIVASVLKSVAQLKPKTNVSAIMCITDNRVNGDASLPDSVWTSMSGKTVEINNTDAEGRLVMADGLYYGADVLKATRLIDVATLTGAMVAALGDTYTGI", "aa_hexdigest": "3394784bffc5a402b5bc81c8a5fdddbf", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002201A65A", "query_cov": 0.9365853658536586, "subject_cov": 0.8170212765957446, "identity": 0.594, "score": 222.0, "evalue": 2.96e-70, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_UPI002201A65A" }, "pscc": { "uniref50_id": "UniRef50_UPI002201A65A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "product": "M17 family metallopeptidase" }, "genes": [], "id": "LMJBGEANGM_1008", "locus": "GCA013368015_01008" }, { "type": "cds", "sequence": "contig_1", "start": 585990, "stop": 586439, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAATTAAACAATTATCAACAAAACGCAATGATCATTTGTTATTGAAAGCAATATTTGAAGATGAAATTTTGCCAGAATTTTTAGTTAAAGAAACAGAAGCAATAACAGATTTTATTGATAAAAAAGTTTCGTATATTTATCTAGGCAAAAAGCAAGAATTACAATACAATTCTTTATATTCATTGGCACAAGATTTAGCATTGAATGCTGCCTACGATTATCAAATTGATCTTTCTTCTTTTGTAACAGAAAATTTAACAATTAATGATGTAGTTGATGCATTCACAAAAGGAATAAATTTCTCAGCTGCAAAGTTATACAATAAAAAGACATTTACATACAAAGAAAATCCAAACAACATAGAATTGTTTATAGAACAAGCGTCTCAATCAGTAATTGATTCATTCAATAAAGCAAAAATATTAATTGAATCTCAAAACTGA", "aa": "MQLIKQLSTKRNDHLLLKAIFEDEILPEFLVKETEAITDFIDKKVSYIYLGKKQELQYNSLYSLAQDLALNAAYDYQIDLSSFVTENLTINDVVDAFTKGINFSAAKLYNKKTFTYKENPNNIELFIEQASQSVIDSFNKAKILIESQN", "aa_hexdigest": "2978e2f0704b330861261012e508d66a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17161.293199999986, "isoelectric_point": 4.910002326965332 }, "id": "LMJBGEANGM_1009", "locus": "GCA013368015_01009" }, { "type": "cds", "sequence": "contig_1", "start": 586453, "stop": 586686, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCCCTACATGATGATTATGATAAACCAAATAAAGAATCTAAAGTAGCTGATCTTAATAATTACAACAACAGTGAAAAATCAGATTGTAATACAGCAGCAATGTTCTTAAAACATTTTACAAATAATGTTGACTATATTCATTGCGATATTGCAGGAACCGCTGACAATAAAGGAGTAGGACTAGGAATTTTAGTTTCTACCTTTGTTGAATTAGCAGCAAATCAAAAATAA", "aa": "MPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK", "aa_hexdigest": "fe1abfe2770c91cf8972a0375caca55b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 58, "aa_cov": 0.7532467532467533, "hmm_cov": 0.9655172413793104, "evalue": 5.234789535188628e-15, "score": 51.002933502197266, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 8490.333599999998, "isoelectric_point": 5.05994358062744 }, "id": "LMJBGEANGM_1010", "locus": "GCA013368015_01010" }, { "type": "cds", "sequence": "contig_1", "start": 586756, "stop": 587817, "strand": "-", "frame": 3, "gene": "pepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "db_xrefs": [ "COG:COG0260", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZXA3", "UniRef:UniRef90_D1J8L9" ], "nt": "ATGAATTCTATTTATTATGAAACTAAACGTAATAAAGATATGCTTTTAACAGCAGTTTATGAAGGCTCAAACGCTAGTTTAAATATTGCCAAGAAAGATAATCATATTACAGAAGATTTTGAAAAAAATGAAGCATTTATTTACATACCAAAAACTGTAGATAACTACTTTAGTTTTTTAAAAGTAGTTGACAAAATTCTAAGTTCAAATAGAAGAAATTATCAAATTAATATTGCTTCTTTTGTTAAAGAAAATGTTGTGTCAACAGAAGAAGTTTTTAGAGCATTTGTATCTAAATTAGCATTTAAAAATGCAAATCTTTATTCAGCAAAAACCAAAGAAAACAAACAAGAAAAGACAATAACATTGTTTTATGCTGATAAATCGTATGAAGAATTAGTTGAAAGATTGTCAATAATTCTTGAAGCAACAAATAAAGCCCGTAGTTTGCAAATGACACCCCCTAATGTTGCAACTAGTGAATATATCGCTAAGGTAGTAAAAGAAGATTTAGAAAATATTCCTGGACTATCAATAAAAGTTTTAGATCGTGATAACATCAAGAAAAATAACATGGGACTTATGTTGGCAGTAAATTCGGGTTCTTCTTATGAACCAAGAGTTTTAATTGCTGAATATAATGGCAATCCTGAAAGTAAAGAAAAATATGTTTATGTAGGAAAAGGAATTACATTTGATACCGGAGGATATAACACCAAAGGATACTACATGGATGGCATGAAATTTGACATGTCTGGTTCTGTTATTGTTGCATATGCAGTTAAAGCAATTGCTCAACTAAAATTAAAAGCAAACGTAGCAGCAATAATGATGATTACAGATAACGCAATAGATACACACCCAACAATGCCTGAATCTGTAATCAAATCAATGAGTGGTTTAACAGTTGAAATTACTGATACTGATGCTGAAGGAAGATTAGTGTTGGCTGATGGTTTATACTACGGTGCCTCGAAATTGAATGCTTCATTATTGATTGATGCTGCAACTTTAACCGGTTCGATGGAAAGAGCATTAGGAAAGACGTATTCAGGAATTTGA", "aa": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFEKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGI", "aa_hexdigest": "05bf4fef855c48fd651185d5b5ef25c1", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pepB" ], "truncated": "5-prime", "pseudogene": { "start": 586453, "stop": 587817, "observations": { "mutation": [ 586692, 586737, 586758 ], "stop": [ 586692, 586737, 586758 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L9", "query_cov": 1.0, "subject_cov": 0.7775330396475771, "identity": 0.997, "score": 672.0, "evalue": 3.39e-242, "gene_start": 1, "gene_end": 353, "reference_start": 1, "reference_end": 353, "reference_sequence": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFKKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGIWSTSEQRWEDFNKAAKIAHEKVWRMPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK" }, "paralog": false, "identity": 0.9889867841409692, "score": 875.0, "evalue": 0.0, "description": "Internal stop codon at 586692, 586737, 586758. Nonsense mutation around 586692, 586737, 586758.", "psc": { "uniref90_id": "UniRef90_D1J8L9", "gene": "PepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "uniref50_id": "UniRef50_A0A448ZXA3", "cog_id": "COG0260", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L9", "UniRef:UniRef50_A0A448ZXA3", "COG:COG0260", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXA3" ], "product": "Cytosol aminopeptidase" } }, "id": "LMJBGEANGM_1011", "locus": "GCA013368015_01011" }, { "type": "cds", "sequence": "contig_1", "start": 587978, "stop": 588268, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175936802.1", "SO:0001217", "UniParc:UPI00158C2BBB", "UniRef:UniRef100_UPI00158C2BBB", "UniRef:UniRef50_A0A454CA46", "UniRef:UniRef90_A0A454CA46" ], "nt": "ATGAAGAAGAGTAAAAAAATAATTTTGTCTATTTTAGGAGCAAGTTCTTTAATTTCATTACCACTAGTAGCAATATCGTGTTCAAAATTGACATCAAAGCAACAACAAGTTAAATCAAAAATAAAGCAATTACTTGATATAGCAAAAAAGATACCATCTGCCGGTGAAATTATGAACCAATATGAAAAAATGATAAAAGATGAAGAAATTGCAAAACTAAATGATCAACAATGCGACGCAATCTTATTTAGCATAGATTTAGTTATTAATCAACTTAAAACTATTAAATAA", "aa": "MKKSKKIILSILGASSLISLPLVAISCSKLTSKQQQVKSKIKQLLDIAKKIPSAGEIMNQYEKMIKDEEIAKLNDQQCDAILFSIDLVINQLKTIK", "aa_hexdigest": "780e969bde057f64c023e4d2bfeea9e5", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158C2BBB", "ncbi_nrp_id": "WP_175936802.1", "uniref100_id": "UniRef100_UPI00158C2BBB", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C2BBB", "RefSeq:WP_175936802.1", "UniRef:UniRef100_UPI00158C2BBB" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C2BBB", "uniref90_id": "UniRef90_A0A454CA46" }, "psc": { "uniref90_id": "UniRef90_A0A454CA46", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA46" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA46" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_1012", "locus": "GCA013368015_01012" }, { "type": "cds", "sequence": "contig_1", "start": 588346, "stop": 588477, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAACAAATAATAGTTTATGCGCCACATCCTTCACCTCTTGCTGCATATAAAGGGTTTTTTGGCTCAGACGTTTTTAAAAAAATAAATTTTAAACTTAAATCATTATCAAAAGATGAAATCGATTGA", "aa": "MSKQIIVYAPHPSPLAAYKGFFGSDVFKKINFKLKSLSKDEID", "aa_hexdigest": "da4804bc9ad74836a84833da086abdaf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4859.6399, "isoelectric_point": 9.457012367248538 }, "id": "LMJBGEANGM_1013", "locus": "GCA013368015_01013" }, { "type": "cds", "sequence": "contig_1", "start": 588664, "stop": 588981, "strand": "-", "frame": 3, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_D1J8M1", "UniRef:UniRef90_D1J8M1" ], "nt": "ATGTCAAAATTTGCTTTTTTAGAATTTTTAAAAAAAGAAACTTCAAAACCATATTTTGAAGTCTTAAAAAAATTAACTATGTTGCCTAATGTAACGCCAAAGCCAAATAAAGTATTTTTTGCATTTAAAAATTTTGATTTTGACAACCTAAAGGTTATTATTATTGGCCAAGATCCTTATCCTAACAAAGGTGATGCAGATGGTATGTGTTTTTCAAGCAATGCAACAAAATGCCCGGCATCTTTAAAGAATATATTTATTGAAATAAAAAATGAATATCCAAATTCAACATTTGAAACTTTTTCACTAAATTATTGA", "aa": "MSKFAFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNKVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNY", "aa_hexdigest": "9d74dcc2c25d94c17c45cdf48adf99f5", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ung" ], "truncated": "5-prime", "pseudogene": { "start": 588361, "stop": 588981, "observations": { "mutation": [ 588585, 588666 ], "stop": [ 588585, 588666 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M1", "query_cov": 1.0, "subject_cov": 0.4861111111111111, "identity": 0.981, "score": 213.0, "evalue": 9.65e-69, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MSKFVFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNYWKNQGVLLLNSILTNIEGKTLAHKNLGWELFNQNLLTEINSCYSNIIYLILGNNAKDFIKNIDMSKQIIVYAPHPSPLAAYKGFFGSDVFKKINFKLKSLSKDGIDWSTKK" }, "paralog": false, "identity": 0.9757281553398058, "score": 407.0, "evalue": 2.03e-147, "description": "Internal stop codon at 588585, 588666. Nonsense mutation around 588585, 588666.", "psc": { "uniref90_id": "UniRef90_D1J8M1", "gene": "Ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_D1J8M1", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M1", "EC:3.2.2.27", "UniRef:UniRef50_D1J8M1", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M1" ], "product": "Uracil-DNA glycosylase" } }, "id": "LMJBGEANGM_1014", "locus": "GCA013368015_01014" }, { "type": "cds", "sequence": "contig_1", "start": 588984, "stop": 589289, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "TTGTTTGAAATTTACAACGGCCAATATAATCGAGTGGCTCAGGATGAATCTAAAGTAACATTTGCTCCTAAGATATTAAATGGTATGGAAAGGATTGATCAAAGCGAAAATCTTTCAAGTGTTGTAAACAAAATCAGAGCTTTTAATGATGAACCAGGTGCTTATTTAATTTTTCAAAATAAAAGATTAAAAATCTTCCGGGCTTCAACAACAAAAATTAGTACCCCATTGAAAATAAAATTTAATGATGGATATTTGTACTTAATAGAATATCAATTTGAAGGGAAAAAAAGAGTAAGAGTTTAA", "aa": "MFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIFQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV", "aa_hexdigest": "e6c751612b4fecbc4246db4a139adee5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 86, "aa_cov": 0.7128712871287128, "hmm_cov": 1.0, "evalue": 1.0419081728580644e-12, "score": 43.66648864746094, "start": 28, "stop": 99 } ], "seq_stats": { "molecular_weight": 11846.478999999987, "isoelectric_point": 9.653898811340333 }, "id": "LMJBGEANGM_1015", "locus": "GCA013368015_01015" }, { "type": "cds", "sequence": "contig_1", "start": 589290, "stop": 589901, "strand": "-", "frame": 1, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "COG:COG0223", "COG:J", "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_B3PNE5", "UniRef:UniRef90_D1J8M2" ], "nt": "TTGCTTGAAATACAAGATAATAAGTTTATAAAAAATAATTTATTTTATAATTTTGATATGAAAAATATAAATAACAAAATTAATTTAGTTCTAGCGGGAACTGGACAATTTTCCGCCAAAACTTTTGAACCTCTTTTAAATGACGAAAGGTTCAATGTTTTGGCATTTATTTCTCAGCCAAATAGGAAACTAGATCGAAACAAAAATGTAATTGAAACAGAAATTTTTAAACTTGCCAAACAGTATGATATAAAAGTTTTTCAACCTCAAAAAATAAAAGAAATTTTGCCCGAACTTAGTGCAATGGATTTTGACTTTTTTATCACAGCTTCATTTGGACAATTTATACCTGATTCAATATTAAAATTACCTAAAAAAATGCCCTTGAACGTTCACGGAAGTTTGCTTGAAAAATATCGTGGAGCTGCGCCTGTGCAATATGCATTACTCAATGGTGATATTGAAACAGGAATAACATTAATTGAAATGGTAAAACAAATGGATGCAGGAGATATCCTAGAATCCTCGAAAGTGAAAATTGATTCTGCAGATACAGCAATTGAAGTATTTGATAAATTAGCAGATGTGGCCTCAAAAAATCTTGGTAATTGA", "aa": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILESSKVKIDSADTAIEVFDKLADVASKNLGN", "aa_hexdigest": "ad9e0328b91e334417c24a773a888dfe", "start_type": "TTG", "rbs_motif": null, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 588987, "stop": 589901, "observations": { "mutation": [ 589292 ], "stop": [ 589292 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M2", "query_cov": 1.0, "subject_cov": 0.6655737704918033, "identity": 0.99, "score": 390.0, "evalue": 1.52e-135, "gene_start": 1, "gene_end": 203, "reference_start": 1, "reference_end": 203, "reference_sequence": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILKSSKVKIDSIDTAIEVFDKLADVASKNLGNWLFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIYQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV" }, "paralog": false, "identity": 0.9835526315789473, "score": 587.0, "evalue": 1.27e-215, "description": "Internal stop codon at 589292. Nonsense mutation around 589292.", "psc": { "uniref90_id": "UniRef90_D1J8M2", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_B3PNE5", "cog_id": "COG0223", "cog_category": "J", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M2", "EC:2.1.2.9", "UniRef:UniRef50_B3PNE5", "COG:COG0223", "COG:J", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_B3PNE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PNE5" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "LMJBGEANGM_1016", "locus": "GCA013368015_01016" }, { "type": "cds", "sequence": "contig_1", "start": 589942, "stop": 590340, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGCAAATAGCAAAGATCTAATTGCAAGGTTAACAATTTTGAAATTCTTGAATCAACAAAAAGATTTATTTCTAGCAAATAATAATGCATTGCCAGCCAATACACCAATTGGAATAAAATATAGTCAATATAATATATTTAATATTAACAACGAATATAAAACACTAAATATTTTAAATGTTTATCAACCTGTAATTTTAAAATATTCAACCATTGCTGAAGCATTCAATCAAAATGGTTTAGATCAATTGTTAAAGAATAATGAATTTGCAAACTATTTTAAATTTTTAGACAGTAGTTACATTCAATTTTCAAATAACGTTTTAAGCAATGATAGTTTAACAAAAATAGGTTCAAAAACAGAAAATTTATTGAACTTCATATTGCCTTGA", "aa": "MKEANSKDLIARLTILKFLNQQKDLFLANNNALPANTPIGIKYSQYNIFNINNEYKTLNILNVYQPVILKYSTIAEAFNQNGLDQLLKNNEFANYFKFLDSSYIQFSNNVLSNDSLTKIGSKTENLLNFILP", "aa_hexdigest": "72ad9c6b90100cee826153b891363ca2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15134.073099999987, "isoelectric_point": 8.776935386657716 }, "id": "LMJBGEANGM_1017", "locus": "GCA013368015_01017" }, { "type": "cds", "sequence": "contig_1", "start": 590584, "stop": 590916, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTGGTTGCCTTAATGTTTTTTTGCGAATATTTTAAAACCGGTCGTTCTTTAGGAACAATTAATAATATAAAAATTGGAGAAGCAAGTCTAAATATAACTTTATACGGAAGTAGTATTAAAGAGATGTTTTCAAGGATATTTTGGCCAGGTTATATATTTAGTTTTGTCATTATAATTTTTGTAAGTATATCTGTTGGGCTATTCATTATAAGCGTTTCAAAATCAGTATTAACTATTCAAGCAATCGGTTGCTCGCTATTAATAATCACAATGTTCTTATCGGGCCAAGTGTTGCCTATTTCTCAAATTTCAAATATAAAAGGAATGTGA", "aa": "MLVALMFFCEYFKTGRSLGTINNIKIGEASLNITLYGSSIKEMFSRIFWPGYIFSFVIIIFVSISVGLFIISVSKSVLTIQAIGCSLLIITMFLSGQVLPISQISNIKGM", "aa_hexdigest": "6bc0413bb7fd0fada78547a2c6e8b369", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12143.528899999987, "isoelectric_point": 9.390352058410645 }, "id": "LMJBGEANGM_1018", "locus": "GCA013368015_01018" }, { "type": "cds", "sequence": "contig_1", "start": 590990, "stop": 591115, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGAGGCACCAATTCTTTTTAAAATTGTGGATTTTTTAAATTCAAATATTGCTGTTGGCAGTGAAATACAGGCTATTGCCACCGGACCAATGGTAATTGTACTTCCTAAAATCATTTCATAA", "aa": "MLEAPILFKIVDFLNSNIAVGSEIQAIATGPMVIVLPKIIS", "aa_hexdigest": "365c3ba57a6f825defea4261b7090197", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4367.260099999999, "isoelectric_point": 4.677076148986816 }, "id": "LMJBGEANGM_1019", "locus": "GCA013368015_01019" }, { "type": "cds", "sequence": "contig_1", "start": 591290, "stop": 592135, "strand": "-", "frame": 2, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_175936809.1", "SO:0001217", "UniParc:UPI00158A15BF", "UniRef:UniRef100_UPI00158A15BF", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_D1J8M4" ], "nt": "GTGGAAAATGAAAAAGTTTGTAAAAATAAAAGTCTAGAAATAATAGACAATTTTTTAAGTAATTTAGAAGATCATGAAAAAAACACAATTAAAGCGAATTCAATTGAAATGCGCCAATACAATTTAAAAAAGTTGGTAAAAATTAAAAATTTGACAAAAGAATTCAAAATAAAAAAAGAAAAAATAAAAGTTGTAGATAATTTAAATCTAGAAATTTATAAGAATCAAAACATTGCTCTATTAGGTGGAAATGGAGCCGGAAAAACTACAACAGTTGAAATGCTTATAGGAATTTTAAAACCTACCTCAGGCAATATTGAATATTATTTTAATGATGGATCAAATCATAATGAAAGTGCACAAACAAACATAGGAATTCAATTTCAGGATTCTTCATATCCTCAAGGTTTATCGGTTAATGATGTCATCTATTCAATGAACAAAATCTATGGCAATAAAAGTTCACCGGAAGAATTACAAAAACTTATAAAGATTTTTGGGGTTGATGAATTTATAAATAATAAAGCTTCTTCCTTAAGCGGGGGCCAACATCAAAGACTTAATGCATTGCTTGCAATAATTAATAAACCAAAGTTGTTAATATTAGATGAATTAAGCACTGGTCTTGACATAAAAATAAAAACAAGATTGGTATCATTTTTGAATGACTATATCAAAGAAATTGATTGTACTTTGATTTTAATATCTCACGATATAAATGAAATTGAAATTCTGGCAGATAGAATTATTATTATGAATAAAGGTAGAATTGTCCTTGATGCTTTGAAAAGTGAAATAATTGAAAAATACGGATCAATATCCCTATGTCTTAAGGAATATATTTAA", "aa": "MENEKVCKNKSLEIIDNFLSNLEDHEKNTIKANSIEMRQYNLKKLVKIKNLTKEFKIKKEKIKVVDNLNLEIYKNQNIALLGGNGAGKTTTVEMLIGILKPTSGNIEYYFNDGSNHNESAQTNIGIQFQDSSYPQGLSVNDVIYSMNKIYGNKSSPEELQKLIKIFGVDEFINNKASSLSGGQHQRLNALLAIINKPKLLILDELSTGLDIKIKTRLVSFLNDYIKEIDCTLILISHDINEIEILADRIIIMNKGRIVLDALKSEIIEKYGSISLCLKEYI", "aa_hexdigest": "02add37d5987f434d5a96df11ab798dd", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158A15BF", "ncbi_nrp_id": "WP_175936809.1", "uniref100_id": "UniRef100_UPI00158A15BF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A15BF", "RefSeq:WP_175936809.1", "UniRef:UniRef100_UPI00158A15BF" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A15BF", "uniref90_id": "UniRef90_D1J8M4" }, "psc": { "uniref90_id": "UniRef90_D1J8M4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J8M4", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "LMJBGEANGM_1020", "locus": "GCA013368015_01020" }, { "type": "cds", "sequence": "contig_1", "start": 592226, "stop": 592843, "strand": "-", "frame": 2, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_D1J8M5", "UniRef:UniRef90_D1J8M5" ], "nt": "TTGGCAATAATAATAACTAAATTAAGAGGGAATGATATAAGAAAAATTGGCTCGCAAAATCCTGGGGCAACAAACGCATTAAGAAATTTCGGAATAAAATTTGCAGCATTAGTTTTTGCTTTTGACATTTTTAAGGCCTTTTTGGCAACAATCATTGCATTTTTAATTAAAAAATATCTAAAAAATCTTGAATATATTTTTCCAATGTTTGCAGGGCTTGGCGCAGTAATTGGGCACGTCTTTCCAGTTTATTTTAAATTTAAAGGTGGCAAAGGAGTAGCCTGCTTTTTTGGAATAATGCTTGCATATAATTTTCTATTATTTTTAATTTTTGTAGCAATATATGTAATACTAGTTTTTTCAACAAAATATGTTTCACTTGCTTCATCAATAAGCTGTGGAATAATTCCTTATTTAGGTTTAATTTATATATTTTATGCAGGAACAATATTCGGTTATATGCAACAAAATGTTGTATATCCCGCGCATGTAATTATTTTAATATTGGCTTCGCTAATAATAGTTGTTAAACATATACCCAATTTTAAACGAATATTCAAACATACAGAACCAAAAATTTTTAGCAAAACAAAAAAGCATAAAAACATTGACTCGTAA", "aa": "MAIIITKLRGNDIRKIGSQNPGATNALRNFGIKFAALVFAFDIFKAFLATIIAFLIKKYLKNLEYIFPMFAGLGAVIGHVFPVYFKFKGGKGVACFFGIMLAYNFLLFLIFVAIYVILVFSTKYVSLASSISCGIIPYLGLIYIFYAGTIFGYMQQNVVYPAHVIILILASLIIVVKHIPNFKRIFKHTEPKIFSKTKKHKNIDS", "aa_hexdigest": "a7dad683394f01a182d2cdb35299cb98", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8M5", "query_cov": 1.0, "subject_cov": 0.8951965065502183, "identity": 0.98, "score": 378.0, "evalue": 8.43e-132, "valid": true, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_D1J8M5", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M5" ], "product": "Glycerol-3-phosphate acyltransferase" }, "genes": [ "plsY" ], "id": "LMJBGEANGM_1021", "locus": "GCA013368015_01021" }, { "type": "cds", "sequence": "contig_1", "start": 592989, "stop": 593366, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036433001.1", "SO:0001217", "UniParc:UPI000512A6E5", "UniRef:UniRef100_A0A6A8Q407", "UniRef:UniRef50_D1J8M6", "UniRef:UniRef90_D1J8M6" ], "nt": "ATGGAATTATTAAAATATAGTTTAATTACAAAAAACGAATTCAATAATGAACCCTCTAAAACGATAGAAACAGAATTTGTTCCATATACCGAAGAAGTTGTTGATGAATTTATTCATATAAATTTTATTGATAATAATAATCAGGAATGTGATTTATTAGTCAATAGCGATACTTTAAAAATAAAATATTCTGGTTCATATATCGAGTTACAAAAAAACAAAAGAGTAAGCAATAAAATTCAAGAATTTTCTCTAGATTTTTATTTAAAAGATGTTTTTATTGAAGGAAACAAGATATCTGCTCATTATGATATTTTAGTTGGTAGTGATGTTATTGCAAGCAATATAATTAACATTGAAACAAAGACCGAAAAATAG", "aa": "MELLKYSLITKNEFNNEPSKTIETEFVPYTEEVVDEFIHINFIDNNNQECDLLVNSDTLKIKYSGSYIELQKNKRVSNKIQEFSLDFYLKDVFIEGNKISAHYDILVGSDVIASNIINIETKTEK", "aa_hexdigest": "30222493c3d8b15db772f468b12470ab", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512A6E5", "ncbi_nrp_id": "WP_036433001.1", "uniref100_id": "UniRef100_A0A6A8Q407", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512A6E5", "RefSeq:WP_036433001.1", "UniRef:UniRef100_A0A6A8Q407" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q407", "uniref90_id": "UniRef90_D1J8M6" }, "psc": { "uniref90_id": "UniRef90_D1J8M6", "uniref50_id": "UniRef50_D1J8M6" }, "pscc": { "uniref50_id": "UniRef50_D1J8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14534.186899999986, "isoelectric_point": 4.650191307067872 }, "id": "LMJBGEANGM_1022", "locus": "GCA013368015_01022" }, { "type": "cds", "sequence": "contig_1", "start": 593347, "stop": 593463, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTAGAAAGGCAACAACATTTGAGGAACATGGCCCTGAACTTGCAAAGGCAATCTATCTTTTTGGGGCTGAATTAGTTTTTAAGAGATTAAATGGAATTATTAAAATATAG", "aa": "MPIRKATTFEEHGPELAKAIYLFGAELVFKRLNGIIKI", "aa_hexdigest": "ee89e1d7e6ceb7981c380db406f08935", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4316.1174999999985, "isoelectric_point": 9.397636985778806 }, "id": "LMJBGEANGM_1023", "locus": "GCA013368015_01023" }, { "type": "cds", "sequence": "contig_1", "start": 593887, "stop": 594003, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F", "UniRef:UniRef90_UPI003594285F" ], "nt": "ATGGAAGGAAAAAAGCTTTCAAAAAGAGATAAAACTTTAAAGCAATTTATTGAAGACTATAAAAATGAAGGCTATCCCGCAGCAGCAATAATGAATTTTTTAGCCTTATTAGGTTGA", "aa": "MEGKKLSKRDKTLKQFIEDYKNEGYPAAAIMNFLALLG", "aa_hexdigest": "57ad0b88b87cbb936a0cd5b0b7ea7e03", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 593839, "stop": 594096, "observations": { "mutation": [ 593889, 594045 ], "stop": [ 593889, 594045 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 1.0, "subject_cov": 0.4470588235294118, "identity": 0.8420000000000001, "score": 63.2, "evalue": 3.54e-12, "gene_start": 1, "gene_end": 38, "reference_start": 32, "reference_end": 69, "reference_sequence": "EHITNTPKQLAIYDAFGWETPRFGHLTIITNMEGKKLSKRDKFLKQFIEDYKNEGYRPDAIFNFLVLLGWTSADKSEVMSHQEII" }, "paralog": false, "identity": 0.8, "score": 136.0, "evalue": 1.32e-43, "description": "Internal stop codon at 593889, 594045. Nonsense mutation around 593889, 594045.", "psc": { "uniref90_id": "UniRef90_UPI003594285F", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003594285F", "UniRef:UniRef50_UPI003594285F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" } }, "id": "LMJBGEANGM_1024", "locus": "GCA013368015_01024" }, { "type": "cds", "sequence": "contig_1", "start": 594043, "stop": 594336, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001A2605D2" ], "nt": "TTGCAAATTTCTAATGAAGAAAAGCAAAGAAGAGAAGAAGCCAAAGAATATTCAATAAGATTAAAATTGCCAATAAATAAAGATTATGCTTGGAATGACTTAGTAAGAGGCAAAATCTCTGTTAATAGTGATGATATAGGAGATTTTGTTATTATGAAAAGTGATGGTTATCCAACATATAATTTTGCAGTTGTTGTTGATGACCACCAAATGGAAATTTCACATGTACTAAGAGGTGAAGAACACATCACAAACACACCAAAGCAATTAGCAATATACGATGCATTTGGATGA", "aa": "MQISNEEKQRREEAKEYSIRLKLPINKDYAWNDLVRGKISVNSDDIGDFVIMKSDGYPTYNFAVVVDDHQMEISHVLRGEEHITNTPKQLAIYDAFG", "aa_hexdigest": "53bd8407b64ab2a01a05f4886d21b0e5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00056C184B", "query_cov": 0.8350515463917526, "subject_cov": 0.8181818181818182, "identity": 0.617, "score": 113.0, "evalue": 9.59e-31, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001A2605D2" }, "pscc": { "uniref50_id": "UniRef50_UPI001A2605D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001A2605D2" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "LMJBGEANGM_1025", "locus": "GCA013368015_01025" }, { "type": "cds", "sequence": "contig_1", "start": 594553, "stop": 594744, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7", "UniRef:UniRef90_UPI002208A1AD" ], "nt": "ATGAAAACTATAAGAACAAGATATGCACCGAGCCCAACTGGGTATTTGCACATTGGTGGTGCAAGAACTGCATTATTTAACTATTTATTTGCAAAACATTTCAATGGCACTTTTGTTTTTAGGCTAGAAGATACTGATGTTGCTAGAAACGTTGTTGGAGGTGAAGAAAGCCAATTAAATAATTTAGCGTGA", "aa": "MKTIRTRYAPSPTGYLHIGGARTALFNYLFAKHFNGTFVFRLEDTDVARNVVGGEESQLNNLA", "aa_hexdigest": "025c80f5c6cbd3489fb6fa238db8d3c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0016AA1FE4", "query_cov": 0.9841269841269841, "subject_cov": 0.8611111111111112, "identity": 0.613, "score": 81.6, "evalue": 3.58e-19, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A5J4P5N7" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 594379, "stop": 594735, "observations": { "mutation": [ 594555 ], "stop": [ 594555 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002208A1AD", "query_cov": 0.9523809523809523, "subject_cov": 0.4444444444444444, "identity": 0.9, "score": 114.0, "evalue": 2.38e-31, "gene_start": 4, "gene_end": 63, "reference_start": 3, "reference_end": 62, "reference_sequence": "MKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFNGQFIFRLEDTDVKRNVPGGEESQLNNLAWLGIIPDESPLNPNPIYGKYRQSEKLDRYWKIANELIEKGFAYKAYDNSDELEQQKKKVMKKEYLVLGMINID" }, "paralog": false, "identity": 0.7711864406779662, "score": 185.0, "evalue": 8.95e-62, "description": "Internal stop codon at 594555. Nonsense mutation around 594555.", "psc": { "uniref90_id": "UniRef90_UPI002208A1AD", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002208A1AD", "UniRef:UniRef50_A0A654IDE7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" } }, "id": "LMJBGEANGM_1026", "locus": "GCA013368015_01026" }, { "type": "cds", "sequence": "contig_1", "start": 595067, "stop": 595609, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8", "UniRef:UniRef90_D1J8M8" ], "nt": "ATGAAAAAACAAGCAATTATGACAAAATTTTTACTTTGTATAGGAAGTGTTGCTCCTGTGTTTGCATTGCCACTAGTTGCGGCAAGCTGTAATAAAACAGCAACCATAACTCTCAATTCAAAAGCGAAAGAATCATTTGAAAAATATGGTTATCACGTTAAGGGTTCTGCACTTGATTTTAATAAAAATAATTATTCAGTAAGTAACCCGATATCGCCAAGAGATTTCTTGTACAAATTAACAAAAAATTTAAAAGATGAAAAAGGAAATCCAATTTACTTTAGAGATGCCGATGGAAATTTATTAAAATTTAAGGGTAAAAAATTGGAAAATGGTGAAATAGAATTCGATAGAGATGCCAACGGAAACTACATTTATGACAAAGAAAATGGATATCCAAGATTGAAAGAAGTATTGAATAATGAACTCGAAGTAATTGACGGAAAACCAGTGCAAAGAGTTTATAGAGAATTTTTTAGCAGATTTTTAGATTTTAATAATATTTCTCCAAATTACGATTTTAGAGTTTTCTCTTTTACATGA", "aa": "MKKQAIMTKFLLCIGSVAPVFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDFLYKLTKNLKDEKGNPIYFRDADGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFT", "aa_hexdigest": "f43ed630c71e024fd4b1fece1be0c6a3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 594764, "stop": 595609, "observations": { "mutation": [ 594805, 594850, 594940, 594973, 595018, 595069 ], "stop": [ 594805, 594850, 594940, 594973, 595018, 595069 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M8", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.9390000000000001, "score": 336.0, "evalue": 8.06e-115, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MKKQAIMTKFLLCIGSVAPMFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDANGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFTWEELINYFPATKNDSRYWPYRDNPRALFLVLYWTLKQNEAAPNWKSDFLDPSIESLLAKGKNRNVNTSIEEAPWPFYKGIMKGNKDAFWSNASEPITAIFEKP" }, "paralog": false, "identity": 0.9395017793594306, "score": 522.0, "evalue": 8.78e-191, "description": "Internal stop codon at 594805, 594850, 594940, 594973, 595018, 595069. Nonsense mutation around 594805, 594850, 594940, 594973, 595018, 595069.", "psc": { "uniref90_id": "UniRef90_D1J8M8", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M8", "UniRef:UniRef50_D1J8M8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8" ], "product": "Variable surface lipoprotein" } }, "id": "LMJBGEANGM_1027", "locus": "GCA013368015_01027" }, { "type": "cds", "sequence": "contig_1", "start": 595776, "stop": 596279, "strand": "+", "frame": 3, "gene": null, "product": "cysteine peptidase family C39 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2", "UniRef:UniRef90_UPI001E465ED2" ], "nt": "ATGATTTGCCACTTTTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAATACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAAAAAATTTCTGATTTTGTGGGGTTTGATACTAACATTTACAAAGTAGATTTTTTAAGTTTTATTTCAAATTTGAATGATGTTTCATTTCCATTTGTCACAATATTGAAAAATGATAATTTCTTGCATTATACAATTGTTAAAAGAATTGATAAAGCTTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAGATAAATTCATAAATGTTGTAGTTATATTAAGCAAAAAATTTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAACACAAAACACAAAGTTAACAATAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTATATTAAATATGTTTTAAGCGTTTTATGA", "aa": "MICHFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDVSFPFVTILKNDNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKDKFINVVVILSKKFKTPNCTKINYNLYLTQNTKLTIISIFLAILFNALMLSSSFYIKYVLSVL", "aa_hexdigest": "aa7037d41c9675bbb5cb3425953615e6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 595719, "stop": 596559, "observations": { "insertion": [ 596541 ], "mutation": [ 595722, 596277, 596328, 596355 ], "stop": [ 595722, 596277, 596328, 596355 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E465ED2", "query_cov": 1.0, "subject_cov": 0.5985663082437276, "identity": 1.0, "score": 323.0, "evalue": 3.07e-110, "gene_start": 1, "gene_end": 167, "reference_start": 20, "reference_end": 186, "reference_sequence": "MWINKQDSVKDCAIYTLQAMICHFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDVSFPFVTILKNDNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKDKFINVVVILSKKFKTPNCTKINYNLYLTQNTKLTIISIFLAILFNALMLSSSFYIKYVLSVLWSENANNRLLSILIIFSWIAILKAITWLFKKIIINKLTFYYEKKLYLLVKERLKFASQKDLQKINQNDFMARILSISTITEYKAGLFLFYLKI" }, "paralog": false, "identity": 0.982078853046595, "score": 524.0, "evalue": 1.13e-191, "description": "Internal stop codon at 595722, 596277, 596328, 596355. Frameshift due to insertion around 596541. Nonsense mutation around 595722, 596277, 596328, 596355.", "psc": { "uniref90_id": "UniRef90_UPI001E465ED2", "product": "cysteine peptidase family C39 domain-containing protein", "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E465ED2", "UniRef:UniRef50_UPI001E465ED2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2" ], "product": "cysteine peptidase family C39 domain-containing protein" } }, "id": "LMJBGEANGM_1028", "locus": "GCA013368015_01028" }, { "type": "cds", "sequence": "contig_1", "start": 596415, "stop": 596870, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAAAAAGACCTTCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTTCAACCATTGCAGAATATAAGGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACACTGCTTTTAGGTCTGTTTAATTATAGAATATTATTAATTTGCTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATGTTCAATAATTATTTTTTAAATTATGAATATAGGACGAACGATTTAAATAATTCGAACTCTAAATTATTTAAATCGTTATTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGA", "aa": "MLVKERLKFASQKDLQKINQNDFMARILSISTIAEYKAGLFLFLFENIIIFISSTLLLGLFNYRILLICLLGISITFAISLFSLKYQSKKATELINLKIKSIKMFNNYFLNYEYRTNDLNNSNSKLFKSLFNTQYNLGIFNETKDSFITFI", "aa_hexdigest": "bf11e502ccd3040442d0c5da65ff26ef", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17665.606799999987, "isoelectric_point": 9.620052909851072 }, "id": "LMJBGEANGM_1029", "locus": "GCA013368015_01029" }, { "type": "cds", "sequence": "contig_1", "start": 596973, "stop": 597263, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCCCCTATTGTAAATTTAGTCTTCCTGATTTCAAATTCTTCAATAAATAAACAAGGAATAATAAGAATAAAATATTTATTAGAAATCACGCAAAACAAGGATGGAAATATATCATTCAAACCCCGGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAAAAATCTTAATTTAACAATTAAAAACAATCTAGAAATATCAGGGAAAAACGGAGTTGGTAAAAGTTTAATTTTGAATGCTATATTTAAAGTTTATTGA", "aa": "MNSPIVNLVFLISNSSINKQGIIRIKYLLEITQNKDGNISFKPRKQNKINILNASINIGSKNVIKNLNLTIKNNLEISGKNGVGKSLILNAIFKVY", "aa_hexdigest": "a6c5da80c8a62c13fb4ca75a6ae7df9e", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10680.47299999999, "isoelectric_point": 10.390707969665527 }, "id": "LMJBGEANGM_1030", "locus": "GCA013368015_01030" }, { "type": "cds", "sequence": "contig_1", "start": 597381, "stop": 597737, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTGCATATGCAAGAAAGACAATATAAAGGAAAATATTTTTCTTAAAAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGGGGAAATAATCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAATATTACAAATGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCTATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTCGCTTTAAAAAAATAA", "aa": "MDCICKKDNIKENIFLKNFKKYNLNNLFLDMNLPLNKYIENGGNNLSSGQKEFIIIMRLFCQKYYFILLDEAFENITNEIFLKLKFLILNYQDRAIFIEISHNKKIVANKRKIFALKK", "aa_hexdigest": "ad3492312072d939909b2d012609c73b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14151.632599999977, "isoelectric_point": 9.444441032409667 }, "id": "LMJBGEANGM_1031", "locus": "GCA013368015_01031" }, { "type": "cds", "sequence": "contig_1", "start": 597796, "stop": 598248, "strand": "+", "frame": 1, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "GO:0004540", "GO:0042254", "GO:0046872", "RefSeq:WP_012855716.1", "SO:0001217", "UniParc:UPI0001BC249E", "UniRef:UniRef100_D1J8N0", "UniRef:UniRef50_A0A448ZWQ4", "UniRef:UniRef90_D1J8N0" ], "nt": "ATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAATCAAAAATAGAATTTGGTGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCTAAAAAGATGAAAAAGTTAAATAACTTACACAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCGTGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGAATTGCTCACCTATTTGGTTTTGATCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTAGATAATATAATGAATAAATTAAACATAAAGAGAATATAA", "aa": "MNRINFKNQSFHRFNFMKDFLDILEESKIEFGAKKSLCVDVMFVTAKKMKKLNNLHRQKNYTTDILSFPLDSQEELNFLDEIELGQIIISPWKIKKQAKEFNHSLRREFCYIFAHGIAHLFGFDHITEEEAKIMNGHVDNIMNKLNIKRI", "aa_hexdigest": "8a34892946feb09a64a863af62f051c6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC249E", "ncbi_nrp_id": "WP_012855716.1", "uniref100_id": "UniRef100_D1J8N0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC249E", "RefSeq:WP_012855716.1", "UniRef:UniRef100_D1J8N0" ] }, "ips": { "uniref100_id": "UniRef100_D1J8N0", "uniref90_id": "UniRef90_D1J8N0" }, "psc": { "uniref90_id": "UniRef90_D1J8N0", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_A0A448ZWQ4", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWQ4" ], "product": "Endoribonuclease YbeY" }, "genes": [ "ybeY" ], "id": "LMJBGEANGM_1032", "locus": "GCA013368015_01032" }, { "type": "cds", "sequence": "contig_1", "start": 598262, "stop": 599107, "strand": "+", "frame": 2, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_A0A501XB37", "UniRef:UniRef90_D1J8N1" ], "nt": "ATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGTTAAATTTTAACCTATCAATAATTACGGATAAACCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTATCAAATCATTTTTATTGATACTCCAGGAATTCACAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAGCAATCGGAACGGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCAGTCATTTCAAAAATTGATTTATTAAATACAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAAGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTTAGATTTGATTAATGAAATTAAAACCTATTGCTACGAAGATGAGCCATTGTACCCTGATGATCAAATTGGGGATATAACATTGAGGTTTATGGCAAAGGAAATAATTAGAGAAACTGCTATAAACAATTTAAAATTTGAATTGCCACACTCAATTGCGGTTGAGATCGAACAATTTGATGATAAAAACGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATTGGAAAAGACGGTCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAATTTTTAATCACAAAGTCTTTTTAAATATTAAAGTAAAAATTAGCGAAGATTGA", "aa": "MKKVCNVCLIGRPNVGKSTFLNNVLNFNLSIITDKPQTTKDNIRGIYNDKDYQIIFIDTPGIHKAENLLSERLNSKSYEAIENSDVVLFLTPANEAIGTGDKFIIEKINETNNENKIAVISKIDLLNTKEEADKKAEELKKLGFEKVFGIGQNLPTSYLDLINEIKTYCYEDEPLYPDDQIGDITLRFMAKEIIRETAINNLKFELPHSIAVEIEQFDDKNEDEDYKIYALIYVKKKSQKPILIGKDGQMIKKISIDSRHKMEKIFNHKVFLNIKVKISED", "aa_hexdigest": "bacc15882ffaad2d5fc286bf4d55e025", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8N1", "query_cov": 1.0, "subject_cov": 0.95578231292517, "identity": 1.0, "score": 543.0, "evalue": 5.6e-195, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_A0A501XB37", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XB37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XB37" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "LMJBGEANGM_1033", "locus": "GCA013368015_01033" }, { "type": "cds", "sequence": "contig_1", "start": 599148, "stop": 599612, "strand": "+", "frame": 3, "gene": null, "product": "Hypothetical lipoprotein (MG045 family)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F", "UniRef:UniRef90_UPI00215D9E7F" ], "nt": "GTGAAACGATTAACTACACATAGAACGAGAGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTTTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAGAAAATAATTACCAAATTAAAACAAAATTATTCATATCACGCCTTTACAAATCTAGATGAGTTTACTAGAGCGATAAATAACGAAAAAGCCGTTGCTGGAGTTGGAAGTGATTATCAAATTGCCCAACTAATTATTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTGTTTAATGATTCAAAATTTAATGATTCATCCCAAATAAAGAAGCTTTATCCAGAAATTGTTAATAAACATTTTGATCTATTTGAAAAATTTATCATTAAAAAAATAAAACAAATAAATCCAAAAAACATCAAGCAAACTGATTGA", "aa": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLIIEKKLRKINFAKLFNDSKFNDSSQIKKLYPEIVNKHFDLFEKFIIKKIKQINPKNIKQTD", "aa_hexdigest": "749b54712f4ffe86df2775e96b032d49", "start_type": "GTG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 599148, "stop": 599915, "observations": { "mutation": [ 599610, 599850, 599904 ], "stop": [ 599610, 599850, 599904 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9E7F", "query_cov": 1.0, "subject_cov": 0.4031413612565445, "identity": 0.948, "score": 277.0, "evalue": 1.2e-90, "gene_start": 1, "gene_end": 154, "reference_start": 1, "reference_end": 154, "reference_sequence": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNNSKFNDSTQIKNLYPEIVNKHFDLFEKFIIKKIRQINPENFQQTDWQNISHTKFLPFIYYDKNNNILGFEIDQKKGIDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKNNENFNNLESLSSWKDIIKTLVSKYKNPRVYWTNWFQDNAMIGQFYGYESLNKLKYFSKQNEWADINHENFKEIINDWVSLVKDATGESIKNSKTNKLATDGQELVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKILIWILMLE" }, "paralog": false, "identity": 0.9254901960784314, "score": 474.0, "evalue": 1.19e-170, "description": "Internal stop codon at 599610, 599850, 599904. Nonsense mutation around 599610, 599850, 599904.", "psc": { "uniref90_id": "UniRef90_UPI00215D9E7F", "product": "Hypothetical lipoprotein (MG045 family)", "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9E7F", "UniRef:UniRef50_UPI00215D9E7F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F" ] } }, "id": "LMJBGEANGM_1034", "locus": "GCA013368015_01034" }, { "type": "cds", "sequence": "contig_1", "start": 599637, "stop": 599852, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTTTATTTATTATGACGAACATAACAATATTTTGGGTTTTGAAATTGATCAAAAAGAGGGAATTGACCACTTTTATGAATTTTTAATTCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAAGTCCAAATAAATTTACTGAAAGAAGCAATTTAAAAAATAATGAAAACTTTAACGATTTAGAAAATTTAGATTCTTGA", "aa": "MPFIYYDEHNNILGFEIDQKEGIDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKNNENFNDLENLDS", "aa_hexdigest": "f166036ac10d6af5bcdd3d368874affb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8619.460199999996, "isoelectric_point": 4.391403388977051 }, "id": "LMJBGEANGM_1035", "locus": "GCA013368015_01035" }, { "type": "cds", "sequence": "contig_1", "start": 600123, "stop": 600293, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAGTTCAATAATAGAGCCGATTAATGGCAAGGCAGATGTTTCAATTATGTATAACGGAGATGCCTTAGATGCTTATTATGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCACATATTGGTTTTAAAAGGCCAAAAAATACTTATATGAATATTGATGCTTGA", "aa": "MVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKNTYMNIDA", "aa_hexdigest": "dbce0eb4a47c3e6f05eeb1e90016b357", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6251.040700000001, "isoelectric_point": 5.554044914245605 }, "id": "LMJBGEANGM_1036", "locus": "GCA013368015_01036" }, { "type": "cds", "sequence": "contig_1", "start": 600414, "stop": 600812, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAATCTAATATCAGAAGGCAACAAATTAAAAAAAGATGAAATTGTTAATGAGCTATTTGCAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTACAGATTATTTTGACTATTTCAAAGAAGCATTTGAAACAATGAATTTACCCATTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTTTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTGGAGATATTCAATCCAGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAA", "aa": "MLNLISEGNKLKKDEIVNELFADKNMSMPKKIKDIKKDFFTDYFDYFKEAFETMNLPIVNNFHSINYTPGFKNVKNFIEKWYFLNKNQEVDKTALEIFNPEFNKKINHRIYQPLDLNLKTKIIDYYFQKTKS", "aa_hexdigest": "c4ef3bad1e18ed3715f9cbc4f83b593f", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15968.241499999986, "isoelectric_point": 9.080645942687987 }, "id": "LMJBGEANGM_1037", "locus": "GCA013368015_01037" }, { "type": "cds", "sequence": "contig_1", "start": 600841, "stop": 601038, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGAAAATAAAATTAAAACACCAAACAAAAAAGAAGAACCAATAATTCAATTAGTAGACGTGGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTATTAGGTCCGTCAGGTTCAGGAAAAACAACCATATTGAGAATTATTGGCGGTTTTGAATGA", "aa": "MENKIKTPNKKEEPIIQLVDVVKEFEDKTVLDNVNLSINKGEFITLLGPSGSGKTTILRIIGGFE", "aa_hexdigest": "1c35952b86a6c04b9beefa48b03e8b0b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 34, "aa_cov": 0.5230769230769231, "hmm_cov": 1.0, "evalue": 6.955695956850703e-11, "score": 38.44517135620117, "start": 31, "stop": 64 } ], "seq_stats": { "molecular_weight": 7200.265399999998, "isoelectric_point": 5.396600914001465 }, "id": "LMJBGEANGM_1038", "locus": "GCA013368015_01038" }, { "type": "cds", "sequence": "contig_1", "start": 601471, "stop": 601830, "strand": "+", "frame": 1, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGAACCAAGAAGTATCAGAAATCATGGATCTTGTTGGTTTAAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGAGGTATGAAACAACGTGTTGCGCTTGCTCGCTCGCTTGTTATTGAACCCGAAATTCTTCTACTAGATGAACCATTAAGTGCTTTAGATGCTAAAATTAGACAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTCATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATCAGAGATGGTAAGGTTGAACAATTTGCTTCGCCAAAAGATTTATACGATTATCCAGTAAATAAATGA", "aa": "MNQEVSEIMDLVGLKGNEKKNVNFLSGGMKQRVALARSLVIEPEILLLDEPLSALDAKIRQKMQTLLRDIQKELKITFIFVTHDRNEALQLSDRIAIIRDGKVEQFASPKDLYDYPVNK", "aa_hexdigest": "954e1337d5f672b866653dc8a03c10ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9747899159663865, "subject_cov": 0.9133858267716536, "identity": 0.517, "score": 122.0, "evalue": 1.15e-33, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "LMJBGEANGM_1039", "locus": "GCA013368015_01039" }, { "type": "cds", "sequence": "contig_1", "start": 601900, "stop": 602142, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAAATTTTCGATACTATTCACAAAGAATTTGAACCAGGAGAAAACCTTGATGCATTAATTAGACCCGAAGATGTTTATGTTACCAAAGTAAAAGCCAAATCTAAATTTATTGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTGGGTAAGGAAGTATATTTAGATTGA", "aa": "MGKIFDTIHKEFEPGENLDALIRPEDVYVTKVKAKSKFIGKIIKSTYSGSYYNYEVLVKNKIIKMETSINYEVGKEVYLD", "aa_hexdigest": "a4241d06e7d04c26a50daf9835bcc07c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9271.616300000002, "isoelectric_point": 7.863031578063965 }, "id": "LMJBGEANGM_1040", "locus": "GCA013368015_01040" }, { "type": "cds", "sequence": "contig_1", "start": 602183, "stop": 602464, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAGGAACTGGCAGAACAAAACGAGATTAACAATGGAAAAACAACTGAAATCAACTTCTTATAAAGAAATAGACAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTGGGTGCAAACAAATTGCAACTTTCTTTGCTAATTCCTTATTTAATATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGTCATCTTGCAAACTGGGAAATAGCAGGTAAATCATCAACTTGA", "aa": "MPRNWQNKTRLTMEKQLKSTSYKEIDKKTKKSSKFAKFFLGANKLQLSLLIPYLIFSILFIILPLILIFIYAFKPLEGQGHLANWEIAGKSST", "aa_hexdigest": "1289d5cd50e81ec7724349004f04a562", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10778.760299999987, "isoelectric_point": 10.106466865539549 }, "id": "LMJBGEANGM_1041", "locus": "GCA013368015_01041" }, { "type": "cds", "sequence": "contig_1", "start": 602465, "stop": 603118, "strand": "+", "frame": 2, "gene": "potB", "product": "Spermidine/putrescine ABC transporter permeaseprotein potB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "nt": "ATGATTATTCTTAGATCAATCGGAACCGGAATATGTGCAGCGTTTTTTGCATTAATTATTGGTTTTCCGTATGCCTATATTATTGCAAGAAGCAAATCAATGATATTTAGAACCTTAGGATTAACGTTAATTCTTAGCCCTCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTGTTTTCGGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCCAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACGCAAACTGTTTTTAAAGTTATATTACCCTACTGTATGAAGGCTATATTGAGCGGTTTTGGAGTTGTCCTTATGATGTCAGCAACATCAATAATTCTTTCAGATAAATTGTTACCAAATGGTAGTCAAAAACAACTTATAGGAAATCTTATAAATCAGTTTGCTAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTGGCTGTTTTGTTAACAATATATGGAATTATTTATGGAATACCTTATTTAATTTCAAAAAGAAAGCAGGGAGGAATATATGAATAA", "aa": "MIILRSIGTGICAAFFALIIGFPYAYIIARSKSMIFRTLGLTLILSPLAIFTISKALAVRGLFSAIFDENELNNNFFLIFGMVYLYIPFMVMPLYQVIKDMPNNIIEASQDLGYSKTQTVFKVILPYCMKAILSGFGVVLMMSATSIILSDKLLPNGSQKQLIGNLINQFANTANPYDLANASTLVIITLAVLLTIYGIIYGIPYLISKRKQGGIYE", "aa_hexdigest": "e85be2a79d3e66b5ba532116f4c918c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00352DB7BA", "query_cov": 1.0, "subject_cov": 0.8037037037037037, "identity": 0.843, "score": 357.0, "evalue": 1.38e-122, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_D1J8N4" }, "pscc": { "uniref50_id": "UniRef50_D1J8N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "product": "Spermidine/putrescine ABC transporter permeaseprotein potB" }, "genes": [ "potB" ], "id": "LMJBGEANGM_1042", "locus": "GCA013368015_01042" }, { "type": "cds", "sequence": "contig_1", "start": 603111, "stop": 603248, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTAAAAAATTTTTGAAATATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATTATTTTTGGAACAATATATAGCTTCAACGCACCTTCTGACAAGGGAATATTTTCTGTAACCTCATGA", "aa": "MNKFKKFLKYSYVLIILACLYIPIIFGTIYSFNAPSDKGIFSVTS", "aa_hexdigest": "40b3e6b142f85f76aa021437701af697", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5156.148800000001, "isoelectric_point": 9.519095420837402 }, "id": "LMJBGEANGM_1043", "locus": "GCA013368015_01043" }, { "type": "cds", "sequence": "contig_1", "start": 603417, "stop": 603668, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACTGGTTTAACGCTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGAGCAATAATTTCGCATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACCTGA", "aa": "MQSTSNIPLINPDVITGLTLAIVLNLIFFTTLKATNEGFFRAIISHTVMTLPYGILIMLPKSDKFSKNIFEASQDLGYSKFKT", "aa_hexdigest": "c816bf02c6619802a6f11e59c2631485", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9241.785499999993, "isoelectric_point": 9.157943534851075 }, "id": "LMJBGEANGM_1044", "locus": "GCA013368015_01044" }, { "type": "cds", "sequence": "contig_1", "start": 603690, "stop": 603821, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCAATAGGATTTACATTTGTAATCACAATGACACTTTCTTTTGACGACTTCATAATTACTAGAATTGTTTCAAATACAGAAACTTTGGGTACTCAATTATATGAAGGCCAATTTCAAGCATGA", "aa": "MLGSIGFTFVITMTLSFDDFIITRIVSNTETLGTQLYEGQFQA", "aa_hexdigest": "2f3fb28131a84edda84bf5710dc59e95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4807.450900000001, "isoelectric_point": 4.0500284194946286 }, "id": "LMJBGEANGM_1045", "locus": "GCA013368015_01045" }, { "type": "cds", "sequence": "contig_1", "start": 603972, "stop": 604307, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCTTTATTCCTTGCTTTTTTAGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCTCCAACCATTATTAATAAATTAAAATCAAAATACAATTTCAAAGAATTTAAAGAAATTAGTGAGTTTACTCAAGCATTATTACAAGATAAGGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGGAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGA", "aa": "MRSRILKAISLIFLPLIFIALFLAFLVIKSTNQYRPSIYNYESYLAPTIINKLKSKYNFKEFKEISEFTQALLQDKAIAGIGSDFQAAQLILDRKIQKIDFSLVYGEKYKD", "aa_hexdigest": "5711953ca1b4a6999f26c7eacde63b4b", "start_type": "TTG", "rbs_motif": "AGGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12894.031299999988, "isoelectric_point": 9.551523017883298 }, "id": "LMJBGEANGM_1046", "locus": "GCA013368015_01046" }, { "type": "cds", "sequence": "contig_1", "start": 604956, "stop": 605072, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAACGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCTAAAAACACATATATGCTAATGGATAATTGA", "aa": "MYNGDALDAFYSEDNFQSVESGLIRFIRPKNTYMLMDN", "aa_hexdigest": "20c0efe0d8ec23b32898ec4eb584397d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.9336, "isoelectric_point": 4.232879447937012 }, "id": "LMJBGEANGM_1047", "locus": "GCA013368015_01047" }, { "type": "cds", "sequence": "contig_1", "start": 605211, "stop": 605363, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACAATTAAAACAATCTGCTGAAGCTTCTTTTTTTGAAACCATACATAATATTGACAAAACTTTAAAAAATAAGTTAATTCAAGAATTTCTAAAAAATAAAGAATTTTTCAATATAAATGAAAAATCAACTAGCTTCGAAGAATGA", "aa": "MTKQLKQSAEASFFETIHNIDKTLKNKLIQEFLKNKEFFNINEKSTSFEE", "aa_hexdigest": "c2205a1656575510b7deb76e03c503f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.699199999997, "isoelectric_point": 6.571235466003418 }, "id": "LMJBGEANGM_1048", "locus": "GCA013368015_01048" }, { "type": "cds", "sequence": "contig_1", "start": 605484, "stop": 605588, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGATGCTTTTGCAAATAGTGAAATAGGAAGCATATCTAATTTTGATTATGTATCATATACTCCAACTGAAAAAATAATTTATGATTTTATTAAAAAATGA", "aa": "MSDAFANSEIGSISNFDYVSYTPTEKIIYDFIKK", "aa_hexdigest": "6a71f98844c169c361765855b19144da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3895.3002000000015, "isoelectric_point": 4.436874580383301 }, "id": "LMJBGEANGM_1049", "locus": "GCA013368015_01049" }, { "type": "cds", "sequence": "contig_1", "start": 605971, "stop": 606516, "strand": "+", "frame": 1, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0004816", "GO:0005524", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_Q4A9S1", "UniRef:UniRef90_D1J8N7" ], "nt": "ATGCAATTAGTTGTTAAGGGAGAACTAGCATCAAAGAAAGAAATCTCTGAAATTGCCATGGGTTCAGCTGTTAGTGTAATAGGTAAATTACACTTAACACCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCTCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCAGGAGAAACTCTACTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTCTAGGGGTAACCGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGGCCAACATTTAGAGCCGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGA", "aa": "MQLVVKGELASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEF", "aa_hexdigest": "177ea8ff0db1f12b3f06a6b8da51d40e", "start_type": "ATG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 605830, "stop": 606819, "observations": { "mutation": [ 605899, 606514 ], "stop": [ 605899, 606514 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8N7", "query_cov": 1.0, "subject_cov": 0.40401785714285715, "identity": 1.0, "score": 356.0, "evalue": 1.75e-120, "gene_start": 1, "gene_end": 181, "reference_start": 48, "reference_end": 228, "reference_sequence": "MNIKQLYKQISQLQENVELTIEGWIISNRGNDKIRFITINDGSTIANMQLVVKGELASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEFWMMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKFLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIVKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQWYLDLRRFGYAPSSGFGLGFERLVMYVTGTSNIRDVIPFPRVAGSIKM" }, "paralog": false, "identity": 0.993920972644377, "score": 640.0, "evalue": 1.43e-234, "description": "Internal stop codon at 605899, 606514. Nonsense mutation around 605899, 606514.", "psc": { "uniref90_id": "UniRef90_D1J8N7", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_Q4A9S1", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0004816", "GO:0005524", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8N7", "EC:6.1.1.22", "UniRef:UniRef50_Q4A9S1", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0004816", "GO:0005524", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A9S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A9S1" ], "product": "Asparagine--tRNA ligase" } }, "id": "LMJBGEANGM_1050", "locus": "GCA013368015_01050" }, { "type": "cds", "sequence": "contig_1", "start": 606517, "stop": 607032, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATGGAACCAGAAGTGGCCTTTTGTGATTTAAAAGGTATCATTGAAATTACAGACGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATTTTTGGATTCATTACAAAACAATAAATTAATCGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGATGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTTGAAGAAAAAGATATCTTTTTCGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCCGAAAACATCATAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTATTAGTACCAGGTATTGGCGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATATCAAAAGCTTGTTGATAGAATCAATGAACTAAAAATCACTCAAGAAACATTACAATGA", "aa": "MMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKFLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIIKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQ", "aa_hexdigest": "526f211966022516ce076ee50ac570ac", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 177, "aa_cov": 0.9824561403508771, "hmm_cov": 0.807909604519774, "evalue": 3.131079399747467e-21, "score": 71.22252655029297, "start": 1, "stop": 168 } ], "seq_stats": { "molecular_weight": 19749.480999999992, "isoelectric_point": 4.737552833557129 }, "id": "LMJBGEANGM_1051", "locus": "GCA013368015_01051" }, { "type": "cds", "sequence": "contig_1", "start": 607183, "stop": 607572, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAATAATTTAACAATATTAATTCCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTGTATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCCAAAACCTTTTTCTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATAATGTATTCATATATTGAAATTGAGTATGATTTTATTTCAAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGA", "aa": "MQENNLTILIPIYHPTLTINEILKNLYKQKQQNFNLVIAIDKPSESELYELEKAKTFFSNRLQIIINTNHQHIDSIISSCLNITKTKYTFIMYSYIEIEYDFISKINKLLLEFKDVDIISFDAYTKGIS", "aa_hexdigest": "832ac88b1f4755a0e7b3b13d06f85896", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15202.394499999984, "isoelectric_point": 5.884904670715332 }, "id": "LMJBGEANGM_1052", "locus": "GCA013368015_01052" }, { "type": "cds", "sequence": "contig_1", "start": 607573, "stop": 607815, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCTAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGATGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGA", "aa": "MNFNKYPKQNKEINIHIEEENVAKIIPFMYNFIAKTSLLIDATNQHNNKHLSEQYSPNILFKTLINANKILLTKDIQVID", "aa_hexdigest": "50b9f594785bef1b8114fb187ab43e40", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9385.73199999999, "isoelectric_point": 8.004862022399902 }, "id": "LMJBGEANGM_1053", "locus": "GCA013368015_01053" }, { "type": "cds", "sequence": "contig_1", "start": 607888, "stop": 608073, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAATTTACAACTCCTTTTTCAGAAAGTTATTATTATCTTGCAAAATTTTTAAATATAGCATATTGTTTCGCAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATTGATAAGTGAAAACATTGAAGAATGA", "aa": "MHEFTTPFSESYYYLAKFLNIAYCFAGFLGQCQFKSNTKNYLTLKNLKKHLESLISENIEE", "aa_hexdigest": "c827a03b589669572e716d6ea1f85952", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7208.160899999997, "isoelectric_point": 6.712196159362793 }, "id": "LMJBGEANGM_1054", "locus": "GCA013368015_01054" }, { "type": "cds", "sequence": "contig_1", "start": 608511, "stop": 608738, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTTTTGCTTTATTTATGATTTTTATATCTCCCCAAACTTTTGAACAAGCAGCAAATATTTCTCAAACAAAACCTTTAGACCAACTTCAAAAGGCTTTTCTTTCAATTCCTAGTGTGCTTTCAGCATTAGCCACATTTTTGGAATTCTTATTTATTTTAAGCAATGCTAAGGGAGATAGAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGA", "aa": "MIIFALFMIFISPQTFEQAANISQTKPLDQLQKAFLSIPSVLSALATFLEFLFILSNAKGDRIGLNDKFSGSYTV", "aa_hexdigest": "0fb0255efc6966c5c6d31f038652c41b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8319.641199999993, "isoelectric_point": 6.000572013854979 }, "id": "LMJBGEANGM_1055", "locus": "GCA013368015_01055" }, { "type": "cds", "sequence": "contig_1", "start": 608963, "stop": 609643, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF00580.27" ], "nt": "ATGATTGATTTAAAAGATTTAAACGAACAGCAAAAAAGTGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGTGCTGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCACTAACTTTTTCTAATAAGGCTGCAAATGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCTTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTATTCAGAACTTGATATTTCACCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAACGATAATAACGAACATTTTAATGAAGACTCAGATTCACAAGAAACACAAGATGAAAAAATCAAGGCAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTTGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTGTTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCCGAAAGATGA", "aa": "MIDLKDLNEQQKSAVIYNEGPLRIIAGAGSGKTRVLTYKIAYLIQKLGVLPNRILALTFSNKAANEMKQRVFNLLAEDNPSNLSPYISTFHAMCAKILRKEIYNFGYENDFQILDELDQKEILKIVYSELDISPTEFTFSSIISFIQNKKNSIEYLNNSINDNNEHFNEDSDSQETQDEKIKAIKEKIYERYQLHLERSHSLDFDDLLVFVYKLFYEPKFSFVAER", "aa_hexdigest": "c8c0bd3435086c36cf028080ebc86a62", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 199, "aa_cov": 0.827433628318584, "hmm_cov": 0.8944723618090452, "evalue": 1.7948668197379516e-08, "score": 29.98868179321289, "start": 7, "stop": 193 }, { "id": "PF13245.12", "name": "AAA_19", "length": 123, "aa_cov": 0.5309734513274337, "hmm_cov": 0.5853658536585366, "evalue": 3.983949572010719e-14, "score": 48.503517150878906, "start": 11, "stop": 130 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 218, "aa_cov": 0.9336283185840708, "hmm_cov": 0.9220183486238532, "evalue": 2.9613034451733673e-50, "score": 166.92657470703125, "start": 7, "stop": 217 } ], "seq_stats": { "molecular_weight": 26346.566800000026, "isoelectric_point": 5.243078804016112 }, "id": "LMJBGEANGM_1056", "locus": "GCA013368015_01056" }, { "type": "cds", "sequence": "contig_1", "start": 609800, "stop": 610003, "strand": "+", "frame": 2, "gene": null, "product": "3'-5' exonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "nt": "ATGAATTTTGATAAGGATTACCCGAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATCTTAAGAGCAGCTAACAACTTAATTTCGCATAATAAACTTAGGTTAGAAAAGAAATTATTCACTGAAAACGAAGAAGGCGAAGATATTGAGTTTTATTGTGGCTTTAATGACGAAGCAGAAGCTAGATGA", "aa": "MNFDKDYPNSVTVKLEENYRSTKTILRAANNLISHNKLRLEKKLFTENEEGEDIEFYCGFNDEAEAR", "aa_hexdigest": "97549997a635dfe7cb9efc2ab9e63df3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00273A74DA", "query_cov": 1.0, "subject_cov": 0.8701298701298701, "identity": 0.5670000000000001, "score": 73.9, "evalue": 5.36e-16, "valid": false, "product": "3'-5' exonuclease", "uniref50_id": "UniRef50_UPI00273A74DA" }, "pscc": { "uniref50_id": "UniRef50_UPI00273A74DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "product": "3'-5' exonuclease" }, "genes": [], "id": "LMJBGEANGM_1057", "locus": "GCA013368015_01057" }, { "type": "cds", "sequence": "contig_1", "start": 610610, "stop": 611200, "strand": "+", "frame": 2, "gene": null, "product": "DNA 3'-5' helicase II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "nt": "TTGCAAGAAATAACTCTAATAACAGATAGAGATGTTGAAGATGATGCCGCAAGTTTCGTTTCACTAATGACTGTTCACAACTCAAAAGGGCTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAACATCTTCCCACTAAAGAGGGCTATATCGATTTCTCCACAAGATGACTTTACTTTTTTAAATTCAAAAAACTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGGCGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCAGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTCTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATTTAATAGTCGGTGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGACGATGAAAGCCTAAATAGTTAA", "aa": "MQEITLITDRDVEDDAASFVSLMTVHNSKGLEFDYVFVACLAENIFPLKRAISISPQDDFTFLNSKNFKENIEGLEEERRLAYVAMTRAKKRLMLSFSVGRNGINRKSRFLSEAGINEIRTIKIANNFSIAAEQETRNNDLIVGDYIIHNTYGKGLIIDMVDNVIEVKFENDKKIRKLSKTHHSIKKYEDDESLNS", "aa_hexdigest": "e6e2bc7dc17bb9e2d01b11770f6fad6b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEJ6", "query_cov": 0.9642857142857143, "subject_cov": 1.0, "identity": 0.677, "score": 244.0, "evalue": 1.45e-79, "valid": false, "product": "DNA 3'-5' helicase II", "uniref50_id": "UniRef50_A0A0F3QEJ6" }, "pscc": { "uniref50_id": "UniRef50_A0A0F3QEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "product": "DNA 3'-5' helicase II" }, "genes": [], "id": "LMJBGEANGM_1058", "locus": "GCA013368015_01058" }, { "type": "cds", "sequence": "contig_1", "start": 611646, "stop": 611828, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTTTTCAATAAAGAAATTCATGATTTGTCTTATATTCCAACCATTGAAAATTATGAAGGCTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATGCTTTTTTAGATCTGTTTAAAGATTTAGGAAAACAACACAATATTAATGGCTTAGAAGTTGTATATGAATGA", "aa": "MDNVFNKEIHDLSYIPTIENYEGLFFFLNKKHITDINAFLDLFKDLGKQHNINGLEVVYE", "aa_hexdigest": "2f4d50a3afdb6ea7115de8983dfb50be", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7118.981400000001, "isoelectric_point": 4.923245811462402 }, "id": "LMJBGEANGM_1059", "locus": "GCA013368015_01059" }, { "type": "cds", "sequence": "contig_1", "start": 612422, "stop": 612616, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCGGTTAATCTAGAGGTTAGTTCTTCATCTATTCAAATGAATTTAACTCAAGGGGATACCAGAGAAATTATTGTTGAATCAATATCCTTAATTTCGAGACAAAAATTGAGTTTTGTTCCTTGTAGTTTAACAATTTTTTGGGATATGTCTTTTATTCTTTCTGCTGATAATAATTTTATTGATTGA", "aa": "MLGSVNLEVSSSSIQMNLTQGDTREIIVESISLISRQKLSFVPCSLTIFWDMSFILSADNNFID", "aa_hexdigest": "134f2509d5cb5e85eff095020be02482", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7143.107599999997, "isoelectric_point": 4.261810493469238 }, "id": "LMJBGEANGM_1060", "locus": "GCA013368015_01060" }, { "type": "cds", "sequence": "contig_1", "start": 612802, "stop": 612957, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAGTAAATAAAAAGTGGGTAGATAGCCATAAAATCCCTGATTCTAAAAGCTCTATAGGTTGCTTTGCACAATTAGATAAAAATATTACTACATTAACTAAAAAAATATTAAACACATGA", "aa": "MNKKLIPIDFFDAVNKKWVDSHKIPDSKSSIGCFAQLDKNITTLTKKILNT", "aa_hexdigest": "d75554f3e6b5d97968a83f29765b6654", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5806.752399999999, "isoelectric_point": 9.519159889221193 }, "id": "LMJBGEANGM_1061", "locus": "GCA013368015_01061" }, { "type": "cds", "sequence": "contig_1", "start": 613129, "stop": 613296, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAGCTCTTTATTTAGATGTAAATTCTACAATTCTACCAGACAAAAGTTTTTATACCGATCCTGCCAAAAAAGAAAAATTTATTAATGTTTGA", "aa": "MELKLSSLNTPLIFSVSTDFKNSDVQALYLDVNSTILPDKSFYTDPAKKEKFINV", "aa_hexdigest": "ec9a7e7705ffe4e81722b014271906cc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6227.049400000002, "isoelectric_point": 4.983893013000488 }, "id": "LMJBGEANGM_1062", "locus": "GCA013368015_01062" }, { "type": "cds", "sequence": "contig_1", "start": 613429, "stop": 614103, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAATTTGCAACACTATTTCAACAAAAGAATTAGCCCAAAACATTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCTGATGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAGTCTTTAACCAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTTGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACGTTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACAGTTGAACACATGATAAAAAGTATGATAGATGTTTATAAAGAACAACTAACAAAAAACTCTTGGTTAAGTAAGCAAACAAAAGAAAAAGCAATAACAAAACTTAACTCAATAGGTGTTTACGTTGGCTTTCCTGAAAAAATTCAAGATCACTATAAATATTTAATAGTTGAAGAATTTGATGGATACAATGATTTATTTGAAAATGTTTTAAAATTTAACAGAATTTTAGCTGAACACATTTTATCTGAATATGGAAAAACAGAAGACAAAAACCTTTGA", "aa": "MVKICNTISTKELAQNIKTINIENILRQLIKCFPDEINAIDPNSINIAKSLTNEENFEYYKASLIIDAILTYAPYFDYSTIQIASEFNLAINGNKKIESKEKYQIKQAKRFFDIPFGTYYGKTYFGLKNKQTVEHMIKSMIDVYKEQLTKNSWLSKQTKEKAITKLNSIGVYVGFPEKIQDHYKYLIVEEFDGYNDLFENVLKFNRILAEHILSEYGKTEDKNL", "aa_hexdigest": "da1324a1b1a56f1616594d17a8d461e9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26152.692500000026, "isoelectric_point": 7.639296913146972 }, "id": "LMJBGEANGM_1063", "locus": "GCA013368015_01063" }, { "type": "cds", "sequence": "contig_1", "start": 614107, "stop": 614316, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCCTGCTATGGTTAACGCCTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCTGCAATCTTACAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTCGGCGGAATTGGTGCTGTTATTGCCCATGAAATATCACACGGATTTGACAATAATGGAGCAAATTTCGATGAAAAAGGAAATATGATTAATTGA", "aa": "MSPAMVNAYYNPSKNVIVFPAAILQKPFFSADQSSSSNFGGIGAVIAHEISHGFDNNGANFDEKGNMIN", "aa_hexdigest": "6ebac6a6f366996c081c11a5317a49ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7351.097399999999, "isoelectric_point": 5.337602043151855 }, "id": "LMJBGEANGM_1064", "locus": "GCA013368015_01064" }, { "type": "cds", "sequence": "contig_1", "start": 614362, "stop": 614532, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTGTTCGAAGGTAGAGAAACATCTGTTGGTAAATGTAACGGTACATTAACTGTTTCAGAAAACATAGCAGATGCTGGTGGTTTATCGTGTGCTTTAGCAGCGGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGA", "aa": "MINLFEGRETSVGKCNGTLTVSENIADAGGLSCALAAAKLEKDYNPKDFYINFATI", "aa_hexdigest": "8a342e6c8964b587198068ef7141ae43", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5986.715900000001, "isoelectric_point": 4.715044593811036 }, "id": "LMJBGEANGM_1065", "locus": "GCA013368015_01065" }, { "type": "cds", "sequence": "contig_1", "start": 614751, "stop": 615662, "strand": "-", "frame": 1, "gene": "tmcAL", "product": "nucleotidyltransferase", "db_xrefs": [ "COG:COG1323", "COG:J", "EC:6.3.1.22", "KEGG:K27343", "RefSeq:WP_175936822.1", "SO:0001217", "UniParc:UPI001588E499", "UniRef:UniRef100_UPI001588E499", "UniRef:UniRef50_A0A3M0A6W2", "UniRef:UniRef90_D1J8P3" ], "nt": "ATGAAAATAGGAATAATAGCTGAATTTAACCCGTTTCACAATGGGCATATTTATTTGATTAATAAAATTAAAGAAATCTTCAAAGATCCCGAAATAATTGTTGCATTATCTTGCGATTATGTTCAACGTGGTGAAATAGCGTGTTTACCTTTTGAAGTTAGAAAAAACATTGCACTTGAATATGGAGCGACAAAAGTTGTTGAATTAGACTTTTTTGCATCTACACAAGCAGCTCATATTTTTGCTAAAAAATCAATAGATTTGTTAATAAAAGAGGGTATTGATTATTTGGTTTTTGGGGTTTCTGACACTGATGATATAAAAAAATATTTAAACGCAGCAAACGTAATAAAGAAAAATTTTGATCAATATAACAAAGATGTAAGAATGAATTTAAAAACAGGAAAATCATATGTTTTGAGTTGTTTCTTGTCGCTAGAAAAATTAATAGGTGTTGAAAATATACCACAAGATATTTTAGGGTTTGAATATACCAAATACATAGTTTTTAATAATTTGTCAGCAAAACCGTTTTGCATAAAAAGAACAGCTCCACACAATTCATTAATAGCAAATAATAATTATGCTTCGGCAACAATGATACGAAAAATGTTAGAAGAAGGCAAAGATGTTTCAATGTTCACACCGATAAACATTCCTAAAAATTTTTCAAAAATTGAAGATAAATATAATGAGTTTAAACAAAAGGTACAAAATTATTCTAAAGAAGAACTGGCAAATATTGCATTAGTATCCGAGGGAATGGAAAATCTTTTTAAAAAGAATATTGAAATAGCAAAAAATTATAATGAATTTGTTGATTTATGTACTTCAAAAAGATATACAAATAGCAGAATTAAACGAGTGATGCTGTATGTTCTTTTAGGAATAAAGAAAGAAGATTTGTGTTAA", "aa": "MKIGIIAEFNPFHNGHIYLINKIKEIFKDPEIIVALSCDYVQRGEIACLPFEVRKNIALEYGATKVVELDFFASTQAAHIFAKKSIDLLIKEGIDYLVFGVSDTDDIKKYLNAANVIKKNFDQYNKDVRMNLKTGKSYVLSCFLSLEKLIGVENIPQDILGFEYTKYIVFNNLSAKPFCIKRTAPHNSLIANNNYASATMIRKMLEEGKDVSMFTPINIPKNFSKIEDKYNEFKQKVQNYSKEELANIALVSEGMENLFKKNIEIAKNYNEFVDLCTSKRYTNSRIKRVMLYVLLGIKKEDLC", "aa_hexdigest": "040567b564805a51493ebe8b2818d6e2", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001588E499", "ncbi_nrp_id": "WP_175936822.1", "uniref100_id": "UniRef100_UPI001588E499", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588E499", "RefSeq:WP_175936822.1", "UniRef:UniRef100_UPI001588E499" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588E499", "uniref90_id": "UniRef90_D1J8P3" }, "psc": { "uniref90_id": "UniRef90_D1J8P3", "gene": "TmcAL", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_A0A3M0A6W2", "cog_id": "COG1323", "cog_category": "J", "kegg_orthology_id": "K27343" }, "pscc": { "uniref50_id": "UniRef50_A0A3M0A6W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M0A6W2" ], "product": "tRNA(Met) cytidine acetate ligase" }, "genes": [ "tmcAL" ], "id": "LMJBGEANGM_1066", "locus": "GCA013368015_01066" }, { "type": "cds", "sequence": "contig_1", "start": 615723, "stop": 615878, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58", "UniRef:UniRef90_A0A454CA58" ], "nt": "ATGTTTAATAAAAAGAAAACAAGCAAGAAAAAACAAATAAAAAAAGAAAAAGAACAAAGCGATATTGTGATGTTCGAAGATTTTGATTCAAACAAAAATTGTGAAGGCGATCAAAAAAACATTGAAATCCAAGAAACTGTGGAATTAAATTATTGA", "aa": "MFNKKKTSKKKQIKKEKEQSDIVMFEDFDSNKNCEGDQKNIEIQETVELNY", "aa_hexdigest": "b74159c3055ddb16e4b2c8f7ccc2c8b8", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 615663, "stop": 615878, "observations": { "mutation": [ 615725 ], "stop": [ 615725 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA58", "query_cov": 1.0, "subject_cov": 0.7183098591549296, "identity": 0.902, "score": 84.0, "evalue": 2.56e-20, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MLNKKKTNKKKQIKKEKEQSDIVMFEDFDSNKKSEDDQKNIEIQETVELNYWEDSSIEDFCDISLDELKKK" }, "paralog": false, "identity": 0.9014084507042254, "score": 128.0, "evalue": 1.33e-40, "description": "Internal stop codon at 615725. Nonsense mutation around 615725.", "psc": { "uniref90_id": "UniRef90_A0A454CA58", "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA58", "UniRef:UniRef50_A0A454CA58" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6129.858499999999, "isoelectric_point": 6.219856834411621 }, "id": "LMJBGEANGM_1067", "locus": "GCA013368015_01067" }, { "type": "cds", "sequence": "contig_1", "start": 616674, "stop": 617126, "strand": "-", "frame": 1, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013142BC0", "UniRef:UniRef90_UPI0013142BC0" ], "nt": "GTGAAAAATACTGAATTTTTGCCTTCAAAAAATGATGTAATTCAGCTAGAAAATGATGATGCTTATATTAATTTAAACAAGGGAAAATTTATGGTTAGAATGGTTGGATTTCACTCTGGATATTTAAACCAAAATAACCATATGTTATTAAACAAAACTATTATTTTGCAGTTTAACAAAAGCCAAAAAGTTTATCAAAATATAGATATCTTAGCCACTAAAACCAGCGAAATTTTAAACAATTTTTATCATGCAAAAACTATTAATTTATCAGGAGATGGAGCAAGAATAATAACTAAATTTAGCGAACAAATTAATGCTATTAGGTATTATGATAAGTTCCATTTTCACAAAATATTATTTGATGTTTTTGGGCATTCAAAGAAGAAAAATAAGGATAATAAGAGAATATTTGATAATACATTTGGGAATCAATTTTCTCAATTTTTATGA", "aa": "MKNTEFLPSKNDVIQLENDDAYINLNKGKFMVRMVGFHSGYLNQNNHMLLNKTIILQFNKSQKVYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGHSKKKNKDNKRIFDNTFGNQFSQFL", "aa_hexdigest": "6ca4bb5274abc3bef8fcd07d77989b3e", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 616371, "stop": 617126, "observations": { "mutation": [ 616553, 616676 ], "stop": [ 616553, 616676 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013142BC0", "query_cov": 1.0, "subject_cov": 0.5905511811023622, "identity": 0.973, "score": 298.0, "evalue": 5.11e-101, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 150, "reference_sequence": "MKNTKFLPSKNDVIQLENDDAYINLNKGKFMVRMVGFHSGYLNQNNHMLLNKTIILQFNKSQKIYQNIDILATKTSEILNNFYHAKTINLSGDGARIITKFSEQINAIRYYDKFHFHKILFDVFGYSRKKNKDNKRIFDNTFGNQFSQFLWFLNNKKYEDFENKLHENLIYLRENKLSAIKIVEIKSLIKLWKNNKNYLINSYENANYYGGNAETFIGHYLKKYTKTAFSRHNIDYLKLKILLNIPQNLNVVFL" }, "paralog": false, "identity": 0.9681274900398407, "score": 494.0, "evalue": 1.24e-180, "description": "Internal stop codon at 616553, 616676. Nonsense mutation around 616553, 616676.", "psc": { "uniref90_id": "UniRef90_UPI0013142BC0", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013142BC0", "UniRef:UniRef50_UPI0013142BC0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013142BC0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013142BC0" ], "product": "Mbov_0401 family ICE element transposase-like protein" } }, "id": "LMJBGEANGM_1068", "locus": "GCA013368015_01068" }, { "type": "cds", "sequence": "contig_1", "start": 618868, "stop": 619221, "strand": "-", "frame": 3, "gene": null, "product": "Mbov_0400 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C3775F7", "UniRef:UniRef90_UPI001C3775F7" ], "nt": "TTGCCATTAAATGAAAGCGATAAATCTTTAATAATTGATGATTTAGAACGAAGGATTAATTCAAATCCACCCGATTTAAATATAACAAAGATTTCAACCCACTTAAAAAATTATTTAGTTTTATATACAACTAATAGTTTAATTTCTACTCAATTGTCTTCTACTCTTGCATATAAAAATACATCAGTTTTGATTAACCAAAAGAATTTTTACAAATATAATTTTAGCAAATCCCAATTTCTTAAAGATACAAACACGTCAAATATAGAATATACTAAGCAAGCCCTAAAAAACATAAGGTTATGCATAAATAAGGCTAAAAAAATGATTATACTAATTTTGAATATATTTTAG", "aa": "MPLNESDKSLIIDDLERRINSNPPDLNITKISTHLKNYLVLYTTNSLISTQLSSTLAYKNTSVLINQKNFYKYNFSKSQFLKDTNTSNIEYTKQALKNIRLCINKAKKMIILILNIF", "aa_hexdigest": "734f4a09ac3c66154d2b9dc569351615", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 618868, "stop": 619521, "observations": { "mutation": [ 619320 ], "stop": [ 619320 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001C3775F7", "query_cov": 1.0, "subject_cov": 0.5246636771300448, "identity": 0.991, "score": 217.0, "evalue": 8.49e-70, "gene_start": 1, "gene_end": 117, "reference_start": 107, "reference_end": 223, "reference_sequence": "MSLELWKPLKITNPLLEDIFHLNIDTKFKIHPLIIFENIQNKTYYCIRLQTARKNTTKYNLQIDNSSYQTNEYWKNHKGVAITKDIFIIDKEVLEANIDQNIYQETLPLNESDKSLIIDDLERRINSNPPDLNITKISTHLKNYLVLYTTNSLISTQLSSTLAYKNTSVLINQKNFYKYNFSKSQFLKDTNTSNIEYTKQALKNIRLCINKAKKMIILILNIF" }, "paralog": false, "identity": 0.9953917050691244, "score": 428.0, "evalue": 1.75e-155, "description": "Internal stop codon at 619320. Nonsense mutation around 619320.", "psc": { "uniref90_id": "UniRef90_UPI001C3775F7", "product": "Mbov_0400 family ICE element protein", "uniref50_id": "UniRef50_UPI001C3775F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001C3775F7", "UniRef:UniRef50_UPI001C3775F7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001C3775F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C3775F7" ], "product": "Mbov_0400 family ICE element protein" } }, "id": "LMJBGEANGM_1069", "locus": "GCA013368015_01069" }, { "type": "cds", "sequence": "contig_1", "start": 620909, "stop": 621028, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGTACATAATGGAAATATTTTGACCAACCTTAATAAAGGCATTTCAAACAAAAAATTTTATGTTTCAAATGAAAATATTTTGCAAATTTCAGTTCTTAATGCTTTGAATGCTTGA", "aa": "MFVHNGNILTNLNKGISNKKFYVSNENILQISVLNALNA", "aa_hexdigest": "82dd6818bbad470fa00772412e88bc9d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4346.960500000001, "isoelectric_point": 9.52476863861084 }, "id": "LMJBGEANGM_1070", "locus": "GCA013368015_01070" }, { "type": "cds", "sequence": "contig_1", "start": 621557, "stop": 621658, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAGCCTCCATATACAAATGCACAATTAATTGATGTTTATATTGATGAAAATCTTATGGAAAATGGTAATGCATTAACTGAAGAGTTTAATTCATGA", "aa": "MLKPPYTNAQLIDVYIDENLMENGNALTEEFNS", "aa_hexdigest": "117989491d30db8d792cb83d06b6ad59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3788.1720000000005, "isoelectric_point": 4.0500284194946286 }, "id": "LMJBGEANGM_1071", "locus": "GCA013368015_01071" }, { "type": "cds", "sequence": "contig_1", "start": 621695, "stop": 621847, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACATATTTAAAAAATATATTCAATGAAAATCCTGACAAGGAAATTTTAGTAATAGATAGATGCGCAGGAACAGGACAACTTGAATATAATCTACCAAAAGATATTCTAAAATGCTTTGTTTTGAATACATATGAATTTTGTGAATGA", "aa": "MQTYLKNIFNENPDKEILVIDRCAGTGQLEYNLPKDILKCFVLNTYEFCE", "aa_hexdigest": "4650754343dda69ca6293300759698d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5888.720099999998, "isoelectric_point": 4.6703691482543945 }, "id": "LMJBGEANGM_1072", "locus": "GCA013368015_01072" }, { "type": "cds", "sequence": "contig_1", "start": 621920, "stop": 622279, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTAAAGATCTTGTTTTAAAAATAACTCCGACCAAAAATGATGAAAAATATTTATTAACAAAACTTAATATTAATAATTTTCACAACATAAATGACAAGTTTTATGAAAAACTAGAACTAGAAAATGATTCTAAATTTATTCAAACCCCTGATATTTTTATAAAAGATTTATTTAAAGGAATGAAAGATATAGAAAAGTGTGTAGAAAATTCTGGTTTAAATGTACAAAACAATTTAGATAAAATAAATAAATTAAAATCTGCGCTAATGCAATTAAATCAAGAAAAAAAGATTTTATATTGGGTAGAACCTATTTTTATTAATGATTTAAAAGAAATAATTGCAGAAAAATGA", "aa": "MNPKDLVLKITPTKNDEKYLLTKLNINNFHNINDKFYEKLELENDSKFIQTPDIFIKDLFKGMKDIEKCVENSGLNVQNNLDKINKLKSALMQLNQEKKILYWVEPIFINDLKEIIAEK", "aa_hexdigest": "dc346bf15e900f00a38d3d618b34a57e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14072.217399999983, "isoelectric_point": 6.593061637878418 }, "id": "LMJBGEANGM_1073", "locus": "GCA013368015_01073" }, { "type": "cds", "sequence": "contig_1", "start": 623296, "stop": 623649, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCAAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGGCTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAGAAAATTCTTTTATTACCTACAAACAAAACGATCGGGTTTGGATATGGATATATTATCAATAAGGCTAATAAAGAATATAATCCTGCAATTGACCCGGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "c9f7e66eb051396304f5653fddd5b1cc", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.95, "score": 178.0, "evalue": 3e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 623014, "stop": 623625, "observations": { "mutation": [ 623115, 623202, 623205, 623298 ], "stop": [ 623115, 623202, 623205, 623298 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.991, "score": 208.0, "evalue": 3.74e-66, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.9655172413793104, "score": 388.0, "evalue": 2.59e-139, "description": "Internal stop codon at 623115, 623202, 623205, 623298. Nonsense mutation around 623115, 623202, 623205, 623298.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13938.78549999998, "isoelectric_point": 5.6632894515991214 }, "id": "LMJBGEANGM_1074", "locus": "GCA013368015_01074" }, { "type": "cds", "sequence": "contig_1", "start": 623650, "stop": 624177, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAATGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCCGAAGATGTTGTTGATGAAAGATATAGAAAAGCAATTGAAAAAGAAGTAATAAATTTCATTACAAATCAATTATTGCAATATCAGCTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTACTAAATAAAGACGTTGAAATTGCTAATTTAAATGAAGTAATTGCTCATTTGATGAGAGGAAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCGTCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "c78174b483990cbd3b8baaf8733ddac8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20132.023300000004, "isoelectric_point": 8.923278617858887 }, "id": "LMJBGEANGM_1075", "locus": "GCA013368015_01075" }, { "type": "cds", "sequence": "contig_1", "start": 624211, "stop": 624435, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAGAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTCCTAGAAGAGCTTGCTAATGTCTCGATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 623985, "stop": 624435, "observations": { "insertion": [ 624024 ], "mutation": [ 624213 ], "stop": [ 624213 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9932885906040269, "score": 289.0, "evalue": 1.55e-102, "description": "Internal stop codon at 624213. Frameshift due to insertion around 624024. Nonsense mutation around 624213.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "LMJBGEANGM_1076", "locus": "GCA013368015_01076" }, { "type": "cds", "sequence": "contig_1", "start": 624577, "stop": 624930, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358", "UniRef:UniRef50_UPI0027330C60", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef90_UPI001314B421" ], "nt": "TTGGAATTAGTTTCGAAATATGATGATCCTAAACTAAAACAAAACAAGGAATTTTATGAAAAATTACAACAAGCCTTAAAGACTCTACAATTACGCAAAACATTTAAATATGAAGGTAAGGATAAAGAAATTGAATTCGAGCCTAAGGATTTAGATCTTAAAAACCAACTCGAACAATACAAAAACTCTATTATTGAAGAACTCGAAAGAGAAATATATAATAATTCTCTAAATTTAGAAGTTCTATACAAGAAAATTCTTTTATTACCTACAAACAAAACCACCGGGTTTGGATATGGATATATTATCAATAAAGCCAATAAAGAATATAATCCTGCAATTGACCCAGATTGA", "aa": "MELVSKYDDPKLKQNKEFYEKLQQALKTLQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTTGFGYGYIINKANKEYNPAIDPD", "aa_hexdigest": "2441d68643de3e43beeafa22e7dd576b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001314B421", "query_cov": 0.8632478632478633, "subject_cov": 0.9805825242718447, "identity": 0.95, "score": 178.0, "evalue": 4.27e-56, "valid": true, "uniref50_id": "UniRef50_UPI0027330C60" }, "pscc": { "uniref50_id": "UniRef50_UPI0027330C60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0027330C60" ] }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 624544, "stop": 624906, "observations": { "mutation": [ 624579 ], "stop": [ 624579 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005C90358", "query_cov": 0.9316239316239316, "subject_cov": 0.4448979591836735, "identity": 0.972, "score": 204.0, "evalue": 2.48e-64, "gene_start": 9, "gene_end": 117, "reference_start": 1, "reference_end": 109, "reference_sequence": "NPKLKQNKEFYEKLQQALKALQLRKTFKYEGKDKEIEFEPKDLDLKNQLEQYKNSIIEELEREIYNNSLNLEVLYKKILLLPTNKTIGFGYGYIINKANKEYNPAIDPDWKPNPNPDDEDIVSPEDIPKDPNRIKKVVKTWWFPLVVIVGILTFLVASIFIIKSLLRKYGWSFGKRAKRIRRQNPNKQSYQEKRSNKRQEKLAKKEAKNLKRQTKKQEKANFIQAKKDKKTKNKSKTNNEQNSIN" }, "paralog": false, "identity": 0.9666666666666667, "score": 238.0, "evalue": 2.66e-80, "description": "Internal stop codon at 624579. Nonsense mutation around 624579.", "psc": { "uniref90_id": "UniRef90_UPI0005C90358", "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005C90358", "UniRef:UniRef50_UPI0005C90358" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005C90358", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C90358" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13956.757799999981, "isoelectric_point": 5.6632894515991214 }, "id": "LMJBGEANGM_1077", "locus": "GCA013368015_01077" }, { "type": "cds", "sequence": "contig_1", "start": 624931, "stop": 625458, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATATACAAAGTGAATTTATTTTAAATATTTCTAAAAAAACTTTCGAAGAAAAAAACATCCCTGAAGATGTTATTGATGAAAGATATAGAAAAGCAATTGAAAAAGAAGTAATAAACTTCATTACAAATCAGTTATTGCAATATCAACTAAAAACAAAATCTTCTGGCAAAGAAATTGAAATGTTATTAAATAAAGACGTTGAAATTGCTAATTTAAATGAAGTAATTGCTCATTTAATGAGAGGGAAGGCTTTAAGTAGTTTTGTTGGTTTAAAACTAGTTTCTAAAAACGCTAATTTGCATAACTACAAAATAATTAACTTTAAAAATTTAGGCGAAGGAGGTAAGTTTAATTTAAGAAATTTAAGTTACATCTTAATGGATAACAAGAATGTTATTCAAGAAACCAAGCCAAGTCAAATTAGAAAAAAAGTTATTGAAATAGTAGTTAATGCTGCATCTAAATTAGGTCTAGAAATTAACAATGATTATCAAATCTTTGCAATTAATAACGATGCTTATTGA", "aa": "MDIQSEFILNISKKTFEEKNIPEDVIDERYRKAIEKEVINFITNQLLQYQLKTKSSGKEIEMLLNKDVEIANLNEVIAHLMRGKALSSFVGLKLVSKNANLHNYKIINFKNLGEGGKFNLRNLSYILMDNKNVIQETKPSQIRKKVIEIVVNAASKLGLEINNDYQIFAINNDAY", "aa_hexdigest": "16cb6b67b60f78684bdb9d0db65ba5e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20119.024600000004, "isoelectric_point": 8.923278617858887 }, "id": "LMJBGEANGM_1078", "locus": "GCA013368015_01078" }, { "type": "cds", "sequence": "contig_1", "start": 625492, "stop": 625716, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823", "UniRef:UniRef90_UPI001314F823" ], "nt": "ATGTCAAGCAACACATTAACCAAGAATTTAATTGAAAATAAAGATTGGTTTATTTCTGAATTTAGCAACATTACCGAGGAAAATAAATTTCTAGAAGAGCTTGCTAATGTCTCAATTTATGGCACAGAACAAAACCAAAATTATGAAGGTGTTAAACTAACATTAATTGGTAAAGGTTTGTATGCTAATTCTAGAAAAGAAATTTATTTTAGAAATGAAGCATGA", "aa": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEA", "aa_hexdigest": "4cf89e55fbd43bc2c9828114fb6c5739", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 625266, "stop": 625716, "observations": { "insertion": [ 625305 ], "mutation": [ 625494 ], "stop": [ 625494 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314F823", "query_cov": 1.0, "subject_cov": 0.4966442953020134, "identity": 1.0, "score": 145.0, "evalue": 1.93e-43, "gene_start": 1, "gene_end": 74, "reference_start": 1, "reference_end": 74, "reference_sequence": "MSSNTLTKNLIENKDWFISEFSNITEENKFLEELANVSIYGTEQNQNYEGVKLTLIGKGLYANSRKEIYFRNEAWHVYNPPIDLSTLDIQNEFILNISKKTFEEKNIPEDVVDERYRKAIEKEVINFITNQLLQYQLKQNLLAKKLKCY" }, "paralog": false, "identity": 0.9798657718120806, "score": 287.0, "evalue": 1.27e-101, "description": "Internal stop codon at 625494. Frameshift due to insertion around 625305. Nonsense mutation around 625494.", "psc": { "uniref90_id": "UniRef90_UPI001314F823", "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314F823", "UniRef:UniRef50_UPI001314F823" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314F823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314F823" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8600.459199999998, "isoelectric_point": 4.854925346374511 }, "id": "LMJBGEANGM_1079", "locus": "GCA013368015_01079" }, { "type": "cds", "sequence": "contig_1", "start": 626143, "stop": 626376, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGAATGATTTTGGTATTTTTGCAGAGGGTGCTGTCCTTGGTAAAGGAGCCTTAAGAAATCTAATTTTAGATAAAAATATTGCCAAAAAATCTAATGTTACATACTATAAATGCAAACTTTATGATAAAGGAAGCAACAAATGGCTTAGTCCTAGTGAAATTAAGCCTGAACCATTATCTTCATCAAACGGTAATAGTGAAAATGCTTATATGTCTGAAGAAGGCATCTGA", "aa": "MLNDFGIFAEGAVLGKGALRNLILDKNIAKKSNVTYYKCKLYDKGSNKWLSPSEIKPEPLSSSNGNSENAYMSEEGI", "aa_hexdigest": "1867bca5cd9d8bd8af93df2df8149266", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8459.5085, "isoelectric_point": 7.798563194274903 }, "id": "LMJBGEANGM_1080", "locus": "GCA013368015_01080" }, { "type": "cds", "sequence": "contig_1", "start": 627088, "stop": 627585, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGCATTTCATCTACAAAATTCAAGTCAAGAAATTATTATAGATTCTGATACTATGGGAAAATTAACGACAGGTATTGATTTAAATATTTTTAAAGGTGGTAAAAGTAATCTTGAAAAACTACAAGAAATTGCTGAACAAATGGATAATGAAACATCAATTGGCAATTTTATTATTAAATATGGTTATGAAGTCAAACCTATTTCAGATGGAAATCATTTTAATCTAATTTTTAAAATCAAACAATTAAAATATAAGGGAAAAGATATCACTAATTCTTTTGCTTCTAAAATTTTTAATGAAGCAGATACTTATAACTTTGCTAATAATTTTGCTAAAAATCAACAAAGTAAAATTCAAATTAAGAATCTTTATCATATGTTTTATAGTCTTTTTAAAGGCAAGACAATAATGGCTAATGTTCCAGTAAGATTTACTCCAAGCCAAATTTATCAAACTTATGCAGACAAAACAGGATTTGTTAGTAGATTATGA", "aa": "MSAFHLQNSSQEIIIDSDTMGKLTTGIDLNIFKGGKSNLEKLQEIAEQMDNETSIGNFIIKYGYEVKPISDGNHFNLIFKIKQLKYKGKDITNSFASKIFNEADTYNFANNFAKNQQSKIQIKNLYHMFYSLFKGKTIMANVPVRFTPSQIYQTYADKTGFVSRL", "aa_hexdigest": "f15d6edbce881d03bb3eb53d896d5c7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18879.321699999982, "isoelectric_point": 9.260125923156735 }, "id": "LMJBGEANGM_1081", "locus": "GCA013368015_01081" }, { "type": "cds", "sequence": "contig_1", "start": 628363, "stop": 628557, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39", "UniRef:UniRef90_UPI001E2EDE39" ], "nt": "ATGAAGAAAATTAAAAAAACCTTATTGATAGCCGGAGGAATATTTAGTATTGCTTCGGCTTCTCTATTTTCTTTGAGTTTTTCTAGAAATGAACATTATTCAAGCTATAAATATGAAGAAATTGACAAAGACCAATATTCTTTTTTAAACAACAAAAATTTATTAAAAAGTTTTTTTACAAATTTAACATATTGA", "aa": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTY", "aa_hexdigest": "3c30da2aa9a547cf19278edfa2c4f5b6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 628260, "stop": 628557, "observations": { "insertion": [ 628296 ], "mutation": [ 628365 ], "stop": [ 628365 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EDE39", "query_cov": 1.0, "subject_cov": 0.6530612244897959, "identity": 1.0, "score": 123.0, "evalue": 1.85e-35, "gene_start": 1, "gene_end": 64, "reference_start": 1, "reference_end": 64, "reference_sequence": "MKKIKKTLLIAGGIFSIASASLFSLSFSRNEHYSSYKYEEIDKDQYSFLNNKNLLKSFFTNLTYKKNSRQDSFIFNPKSYSGNPLYSIKDLLQTKMIT" }, "paralog": false, "identity": 0.9387755102040817, "score": 174.0, "evalue": 6.97e-58, "description": "Internal stop codon at 628365. Frameshift due to insertion around 628296. Nonsense mutation around 628365.", "psc": { "uniref90_id": "UniRef90_UPI001E2EDE39", "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EDE39", "UniRef:UniRef50_UPI001E2EDE39" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EDE39", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EDE39" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7455.452299999998, "isoelectric_point": 9.477255439758302 }, "id": "LMJBGEANGM_1082", "locus": "GCA013368015_01082" }, { "type": "cds", "sequence": "contig_1", "start": 628544, "stop": 628861, "strand": "-", "frame": 2, "gene": null, "product": "Single-stranded DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66", "UniRef:UniRef90_A0A454CA66" ], "nt": "TTGCCCTTTGTTATTGCACTATTTGAACAAGCAAATAAAACCAATTTTATCGAGATTACTTGTTTTAACAAATTGGCAGAACATGTTATGAAATATGTTAAAAAGGGAGATTTATTAGAAGTTGTTGGCAAATTACACAATGCTAAATTTAGTTCAAAGAATAACGAAACTATTTATAAAACAGAAATAATTGCTAAAAATATTACTTTTTTAACAAAGTCAAAATCAAATATTCAACCCATTTCAAAAGAGACCAACTTAGAAAATAACGAAAAAGATATTTTTGGAATAGATTTTTTTGAAGATGAAGAAAATTAA", "aa": "MPFVIALFEQANKTNFIEITCFNKLAEHVMKYVKKGDLLEVVGKLHNAKFSSKNNETIYKTEIIAKNITFLTKSKSNIQPISKETNLENNEKDIFGIDFFEDEEN", "aa_hexdigest": "58a4eb02bd2fc23367e361be493987a8", "start_type": "TTG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A454CA66", "query_cov": 1.0, "subject_cov": 0.8076923076923077, "identity": 0.981, "score": 199.0, "evalue": 2.55e-64, "valid": true, "product": "Single-stranded DNA-binding protein", "uniref50_id": "UniRef50_A0A454CA66" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA66", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA66" ], "product": "Single-stranded DNA-binding protein" }, "genes": [], "id": "LMJBGEANGM_1083", "locus": "GCA013368015_01083" }, { "type": "cds", "sequence": "contig_1", "start": 629138, "stop": 629302, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD", "UniRef:UniRef90_UPI00138A25BD" ], "nt": "ATGTCTATTATTAATTTAAAACTTAATATGATTATTAATGCTTTATTTAAAGATAATGGAGATTTAGAAAAACAAATTGATAATCCCGGGGCTGATATATTAGCAGAATCTAAGTTCTTTGAACGCACTATAAGAGGTGCTGTTAATAAATTAACTAATGATTGA", "aa": "MSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTND", "aa_hexdigest": "6426191af12777e3dadae3b37578ec71", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 629129, "stop": 629362, "observations": { "mutation": [ 629140 ], "stop": [ 629140 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00138A25BD", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 110.0, "evalue": 1.14e-30, "gene_start": 1, "gene_end": 54, "reference_start": 22, "reference_end": 75, "reference_sequence": "MSALSPYQLQINRRFRSMDIEMSIINLKLNMIINALFKDNGDLEKQIDNPGADILAESKFFERTIRGAVNKLTNDWTKNGK" }, "paralog": false, "identity": 0.987012987012987, "score": 149.0, "evalue": 2.01e-48, "description": "Internal stop codon at 629140. Nonsense mutation around 629140.", "psc": { "uniref90_id": "UniRef90_UPI00138A25BD", "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00138A25BD", "UniRef:UniRef50_UPI00138A25BD" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00138A25BD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00138A25BD" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6063.910899999999, "isoelectric_point": 5.175554084777831 }, "id": "LMJBGEANGM_1084", "locus": "GCA013368015_01084" }, { "type": "cds", "sequence": "contig_1", "start": 629507, "stop": 629773, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAACTAATAAAAACGAATTAGATCAAGATTTTGATAATTTAGATAATAATGAAATTTTATCAAATGACAATCAAACTCAAAATGAAGATGACTTTATAGAAAATATTAATTTAACAAGTGATAATCAAATAAATAACATTATGAATTCTATCACTAGTTCCCAACCTAAAAAACAAAAGGAAAGAAAGCAACAAAACAGTTTTGAAGCACAATTTTCAGGACGATTTACTGATCCTGAAGATGTAGGAATGTTTTATCGTTGA", "aa": "MKTNKNELDQDFDNLDNNEILSNDNQTQNEDDFIENINLTSDNQINNIMNSITSSQPKKQKERKQQNSFEAQFSGRFTDPEDVGMFYR", "aa_hexdigest": "d7af23c1ff69375d467c8d5d18f2c2c4", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10339.962499999996, "isoelectric_point": 4.283920860290528 }, "id": "LMJBGEANGM_1085", "locus": "GCA013368015_01085" }, { "type": "cds", "sequence": "contig_1", "start": 629831, "stop": 630322, "strand": "-", "frame": 2, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9", "UniRef:UniRef90_A0A454C9F9" ], "nt": "ATGTTAAAGAAAAAATATTTGCAAGAACATAACATTAAGTTAATGGATGCAAAGCAAGTAGAAGATACTATTAGATTTTGCGCCGTTATCGTAGATGAAGCCCATTTATATATAGATCCTGATAATCCTGCTATTTTAAAATATATGGTTCAAATGACAAAAACGGTTAGAAAATTCAATGCAGGTATGATATTTACTACCCAAAATCCTGGTGATTTTACTATTAATAATGCCACAAGTGATGATGCATCTAGAATTATTCAAAACTGTCAATATTCTGTATTCTTTGGCCTAAAAGACCAAGATATTGACAAAGTGCAACAATTATTTAAAAACTCTAATCAATTATTACAAAGTGAAATTAGATATTTAGTAAATGCAAGATATGGAAAATGTTTATTTTCAATAAGTTCAAATAAAAGAATTAGAGTTGAAATTTATTACAACAAATTAGAACAAGAATTATTCTTCAAGTTCTTAAATAAAGAATGA", "aa": "MLKKKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTINNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKE", "aa_hexdigest": "78bdbcb7dd1dde5516f8b3f6d42bd74e", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 629831, "stop": 630622, "observations": { "mutation": [ 630337 ], "stop": [ 630337 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F9", "query_cov": 1.0, "subject_cov": 0.5174603174603175, "identity": 0.988, "score": 322.0, "evalue": 3.56e-109, "gene_start": 1, "gene_end": 163, "reference_start": 151, "reference_end": 313, "reference_sequence": "MSWLESFFKVLINIEDHKWILLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNNWTHNEMLKRKYLQEHNIKLMDAKQVEDTIRFCAVIVDEAHLYIDPDNPAILKYMVQMTKTVRKFNAGMIFTTQNPGDFTVNNATSDDASRIIQNCQYSVFFGLKDQDIDKVQQLFKNSNQLLQSEIRYLVNARYGKCLFSISSNKRIRVEIYYNKLEQELFFKFLNKEWI" }, "paralog": false, "identity": 0.9885931558935361, "score": 525.0, "evalue": 8.9e-192, "description": "Internal stop codon at 630337. Nonsense mutation around 630337.", "psc": { "uniref90_id": "UniRef90_A0A454C9F9", "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F9", "UniRef:UniRef50_A0A454C9F9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F9" ], "product": "ATP-binding protein" } }, "id": "LMJBGEANGM_1086", "locus": "GCA013368015_01086" }, { "type": "cds", "sequence": "contig_1", "start": 630335, "stop": 630712, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCAAAATGTAATAAAAGAATTTTATGAAGATAGAGGAATATATAAACTTGCTTCTATTGAGCAATTAAAGGCCGTAGAATGGCCAACAATAAGTGAGTTGATAAAATATATGTCCAACTATAAAACGCTTCATTTAAACAATCCTGAAGCAAAAAGAGCAATCTTAGTTGAACTTTGCGAATCGTTTAGCTTTCTTTTTGAAAATAATGGAAGATATCAAAACTTATTTAATGGACACACTAATATAAAACTTGATAATGATTTTGTAGTATTTAATACGCAAAACTTAATAAATGGAGCGGACAATATCGGCGCTAAATTAGGAACAATGTGTTTATTAAACTTTGTTAGTGAAATAGTATTTAATAATTGA", "aa": "MLQNVIKEFYEDRGIYKLASIEQLKAVEWPTISELIKYMSNYKTLHLNNPEAKRAILVELCESFSFLFENNGRYQNLFNGHTNIKLDNDFVVFNTQNLINGADNIGAKLGTMCLLNFVSEIVFNN", "aa_hexdigest": "b7282ea7acc765500ab8863ad00101c8", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14430.339799999983, "isoelectric_point": 5.328053092956543 }, "id": "LMJBGEANGM_1087", "locus": "GCA013368015_01087" }, { "type": "cds", "sequence": "contig_1", "start": 630764, "stop": 631486, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01656.29", "PFAM:PF01935.23", "PFAM:PF10412.15" ], "nt": "ATGGCATCTATTGATACTACTAATGCAACAGGATCAGTATCGGCAAACTTGGTCGGTTCTATTGATGAACAAGCGACAATGGCAATGATGTATCAAGTAAATAAAGATGAATACAAACTATTTGATAGTAATTTCTTCTTTGTATCAAAAGCCACAGACCTTGCTGAATTAAATAGACAGCATAGAATTGCTAGAAATATAGCAAGTAAAAAGAATATAAGCTTAAATGACTTAAAATATAGACAACTTTTAGGTTTATCTGAGGCTTGCAATTATCCTTTTGAAAGATTAGAAAGAGAATATTATCAAATTACATCTATTAATTTAGCTTTGGGATGGCCTTTTGAAACCGAAAATCACAATGATTCGAATAATTTACTTCTAGGCTACTCAGAAGCCACAAGAAGTCCTCTAAGTTTTAGATTATTTGATATGTATCATTCAAATAGAACTAATTTTAATATGTTTATAAATGGTACTAGTGGAAAAGGTAAAACAACTTTTACCATGAAGTTATTAACTAGTATTTTAGCAGCAAATAATAAAGTTGTTGTTATTGACCCGCAAGGAGAATATAGAGAATTAGCTCAAAAACTTAATGGACAAATTATTGATTTGGGTGCAGGTAAAAACACCATAATCAATCCTCTTCAAATCCGTAATTCATTAAAGGATGCAACGGGTAATGATACATCTTCACTAATTAACAACCATTTGTCGTGA", "aa": "MASIDTTNATGSVSANLVGSIDEQATMAMMYQVNKDEYKLFDSNFFFVSKATDLAELNRQHRIARNIASKKNISLNDLKYRQLLGLSEACNYPFERLEREYYQITSINLALGWPFETENHNDSNNLLLGYSEATRSPLSFRLFDMYHSNRTNFNMFINGTSGKGKTTFTMKLLTSILAANNKVVVIDPQGEYRELAQKLNGQIIDLGAGKNTIINPLQIRNSLKDATGNDTSSLINNHLS", "aa_hexdigest": "cf0d37f42301b4c3b8415fe707399eb9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01656.29", "name": "CbiA", "length": 42, "aa_cov": 0.175, "hmm_cov": 1.0, "evalue": 2.768633856383107e-07, "score": 26.120105743408203, "start": 155, "stop": 196 }, { "id": "PF01935.23", "name": "DUF87", "length": 68, "aa_cov": 0.26666666666666666, "hmm_cov": 0.9705882352941176, "evalue": 2.284801664424129e-10, "score": 36.4718132019043, "start": 157, "stop": 220 }, { "id": "PF10412.15", "name": "TrwB_AAD_bind", "length": 72, "aa_cov": 0.3, "hmm_cov": 0.875, "evalue": 1.4598207798677813e-10, "score": 36.0557746887207, "start": 149, "stop": 220 } ], "seq_stats": { "molecular_weight": 26953.050900000024, "isoelectric_point": 6.912837791442871 }, "id": "LMJBGEANGM_1088", "locus": "GCA013368015_01088" }, { "type": "cds", "sequence": "contig_1", "start": 631580, "stop": 631816, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAAGCAAGACTTCTAACATTTCTTAATAAACTTAATATGTTTGGTATTGATAGCCAAAACATACAAGAATATTGCAATGATTTAGATGCTAAATTTGGTGTTGAAAATTCATTAGATAACTTATTTATTTATAATCAAGTTATTATTAGAGACAAATATTTACAAATCAATGATAATTATGTAAAAATCAATTGTTTAAACAAATTACCATTTGAATTGTCTGATAAATGA", "aa": "MHKARLLTFLNKLNMFGIDSQNIQEYCNDLDAKFGVENSLDNLFIYNQVIIRDKYLQINDNYVKINCLNKLPFELSDK", "aa_hexdigest": "7a39788c74a3b39bfb1c4435ba7fa889", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9232.510099999996, "isoelectric_point": 5.646124076843263 }, "id": "LMJBGEANGM_1089", "locus": "GCA013368015_01089" }, { "type": "cds", "sequence": "contig_1", "start": 632153, "stop": 632290, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTAAATCCTTATTCATGTTTAGTTAATGAATATGTAGTCAAAAATAAAAATTCAAAAATAAGTTTTTATAATCCAAGAAATGATAAAGAAAACTATTTAGCGGTATTTAAACTAAATGGTCTTAATATTTGA", "aa": "MDLNPYSCLVNEYVVKNKNSKISFYNPRNDKENYLAVFKLNGLNI", "aa_hexdigest": "e4028ef62a76857cd24bf8df97f42e76", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5283.9867, "isoelectric_point": 8.852041053771973 }, "id": "LMJBGEANGM_1090", "locus": "GCA013368015_01090" }, { "type": "cds", "sequence": "contig_1", "start": 632627, "stop": 632965, "strand": "-", "frame": 2, "gene": null, "product": "MMCAP2_0566 family conjugal transfer protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A513D7", "UniRef:UniRef90_UPI001314BAAF" ], "nt": "ATGTTGGGTAGTTTTATAGGCTTAGCTAATACATTTATGACTAGTGCTACAAATCATATGATAGGAGCCTTTTCTTCATTATCAAACTTTACTACTATCAAAGCCGTTTTAGCATTATTATCAACTTGTTTCTTGGTAATTGGCTCTGCATTTGCTTTCAAAAAATTATCAGAAATGGCAATTGCTTTTGTTGGAAGTGAAGTTTCTGGTGGCCAATCTGCAAGACTTGCTATGTCTGGAGCAAAATCAGGGGCAGGAGCATATAAAACAGCAAAGGATGCAGCATTAAGGGGCACGAATGTAGGTGCAGCATATAAAGTTGCAAGTTTTCTAAAATAG", "aa": "MLGSFIGLANTFMTSATNHMIGAFSSLSNFTTIKAVLALLSTCFLVIGSAFAFKKLSEMAIAFVGSEVSGGQSARLAMSGAKSGAGAYKTAKDAALRGTNVGAAYKVASFLK", "aa_hexdigest": "cc5a736ee3214dccea3d1f38c7f255d2", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 632627, "stop": 633247, "observations": { "mutation": [ 633025, 633097, 633220, 633226, 633229 ], "stop": [ 633025, 633097, 633220, 633226, 633229 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314BAAF", "query_cov": 1.0, "subject_cov": 0.5384615384615384, "identity": 0.973, "score": 191.0, "evalue": 4.63e-60, "gene_start": 1, "gene_end": 112, "reference_start": 97, "reference_end": 208, "reference_sequence": "MWGLGFPAWWKFTENITGYALTIGFAIAVVGVLISIPLIFVLFDIISRVFINWLLFISFPLIPAFSINDGGKRIRIWKEKYFGNLLSCSIYGVGIMMLGSFIGLANTFMTSAMNHMIGAFSSFSNFTTIKAVLTLLSTCFLVIGSAFAFKKLSEMAIAFVGSEVSGGQSARLAMSGAKSGAGAYKTAKDAALRGTNVGAAYKVASFLK" }, "paralog": false, "identity": 0.9320388349514563, "score": 363.0, "evalue": 4.45e-130, "description": "Internal stop codon at 633025, 633097, 633220, 633226, 633229. Nonsense mutation around 633025, 633097, 633220, 633226, 633229.", "psc": { "uniref90_id": "UniRef90_UPI001314BAAF", "product": "MMCAP2_0566 family conjugal transfer protein", "uniref50_id": "UniRef50_UPI0034A513D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314BAAF", "UniRef:UniRef50_UPI0034A513D7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A513D7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A513D7" ], "product": "MMCAP2_0566 family conjugal transfer protein" } }, "id": "LMJBGEANGM_1091", "locus": "GCA013368015_01091" }, { "type": "cds", "sequence": "contig_1", "start": 633290, "stop": 633556, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314DB3C", "UniRef:UniRef90_UPI001314DB3C" ], "nt": "GTGTTTGTTATTAGGTATTCAAAAGAATCTAATGGATTAAAGGCTAAGGAAATATTTGTTCAGGCCTTAAAATCAACGGTAGGAGGCGTCCTCACGCTAATATTATTCCAAGTAGTAATTCTCTTTATTAATATTATCTTTATTGAAGTAACAAAAGCCTTATATGGAGATTATAACGATTTCTTTAATTTTGGTTTCGGTCAAATTTCAAATAATATTATTAGAAATATGATGCCTCCACAAGCCTTTGTACCTGATAAATATTGA", "aa": "MFVIRYSKESNGLKAKEIFVQALKSTVGGVLTLILFQVVILFINIIFIEVTKALYGDYNDFFNFGFGQISNNIIRNMMPPQAFVPDKY", "aa_hexdigest": "311daec7921bf3dbace72f8ca5aabea6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 633262, "stop": 633826, "observations": { "insertion": [ 633316 ], "mutation": [ 633574, 633607 ], "stop": [ 633574, 633607 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314DB3C", "query_cov": 0.9204545454545454, "subject_cov": 0.4308510638297872, "identity": 0.963, "score": 150.0, "evalue": 2.38e-44, "gene_start": 1, "gene_end": 81, "reference_start": 92, "reference_end": 172, "reference_sequence": "MEFLKAIINPLINVIAAPIRLMWFVPLAAIGLIGFTIFNLLFFLFKLFSLDLNNYLMFNLLPGQSYSQFSVARPWYQFLIISFVLWLINFFVFVIRYSKESNGLKAKEIFVQALKSTVGGVLTLILFQVVILFINIIFIELTKALYGDYNDFFNFGFGQISNNIIRNMMPPKPLYLQNIEIHSLTDLL" }, "paralog": false, "identity": 0.946524064171123, "score": 343.0, "evalue": 7.26e-123, "description": "Internal stop codon at 633574, 633607. Frameshift due to insertion around 633316. Nonsense mutation around 633574, 633607.", "psc": { "uniref90_id": "UniRef90_UPI001314DB3C", "uniref50_id": "UniRef50_UPI001314DB3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314DB3C", "UniRef:UniRef50_UPI001314DB3C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314DB3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314DB3C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10060.749199999991, "isoelectric_point": 9.04899196624756 }, "id": "LMJBGEANGM_1092", "locus": "GCA013368015_01092" }, { "type": "cds", "sequence": "contig_1", "start": 634077, "stop": 634196, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATCGATGATAAAAGCTTTAGCTTTTTAGGAAATTTTAGTGAAACTATAATGAGGTCTATTTTTATAGCCCTTATTCCAGTTGCAATTATTTTATTAATAGGAATGATATTGTGA", "aa": "MKIDDKSFSFLGNFSETIMRSIFIALIPVAIILLIGMIL", "aa_hexdigest": "09fe57378c087bc16488d181ce391d30", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4372.344299999998, "isoelectric_point": 5.876663017272949 }, "id": "LMJBGEANGM_1093", "locus": "GCA013368015_01093" }, { "type": "cds", "sequence": "contig_1", "start": 634416, "stop": 634592, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAATTGAATTTGATAATCTAGAAAGTGGTATGTTAAATAGAAATAGAAAAGATTCATATATTTATGACTATATCTTTAAATATTTAAATGTCATTTTTAAGGCTAAATACAAAGACATTGATGAAATCAAAAAACCAAATGGATTTTTAGATGTAAATAATAAAGAACATTGA", "aa": "MPIEFDNLESGMLNRNRKDSYIYDYIFKYLNVIFKAKYKDIDEIKKPNGFLDVNNKEH", "aa_hexdigest": "f3bcb0baaa97bc2c2c8c4255d44b728f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7048.959700000001, "isoelectric_point": 6.546851539611817 }, "id": "LMJBGEANGM_1094", "locus": "GCA013368015_01094" }, { "type": "cds", "sequence": "contig_1", "start": 634596, "stop": 634688, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1", "UniRef:UniRef90_UPI001E44B7D1" ], "nt": "ATGGGCGACAAAATTGACTTTGATTACACTTATAGATTTGCATATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTAGCAAATGGTTGA", "aa": "MGDKIDFDYTYRFAYKFLYEKLVPTMVANG", "aa_hexdigest": "aec741b8d9ba2cf80992a7ddf680f4ca", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 634548, "stop": 634703, "observations": { "mutation": [ 634598 ], "stop": [ 634598 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E44B7D1", "query_cov": 1.0, "subject_cov": 0.5660377358490566, "identity": 1.0, "score": 65.9, "evalue": 9.65e-14, "gene_start": 1, "gene_end": 30, "reference_start": 6, "reference_end": 35, "reference_sequence": "MKSLTMGDKIDFDYTYRFAYKFLYEKLVPTMVANGWNMPGEFDVLEKNMLDEK" }, "paralog": false, "identity": 0.8823529411764706, "score": 92.4, "evalue": 5.61e-27, "description": "Internal stop codon at 634598. Nonsense mutation around 634598.", "psc": { "uniref90_id": "UniRef90_UPI001E44B7D1", "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E44B7D1", "UniRef:UniRef50_UPI001E44B7D1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E44B7D1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E44B7D1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3597.119100000001, "isoelectric_point": 5.918780708312987 }, "id": "LMJBGEANGM_1095", "locus": "GCA013368015_01095" }, { "type": "cds", "sequence": "contig_1", "start": 634902, "stop": 635084, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAATAAATTCCCACAAGAAACTTATGATATGTATACTGATAAAAATGGTCTAGATAAAACAACTCTTACTCCTGCTTATAATAACTACAATCCTGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAAATTAAATTTCAAAGTTTATAAGAATTTAATTCAATGA", "aa": "MRNKFPQETYDMYTDKNGLDKTTLTPAYNNYNPVAFNFLVMERFDLSEKLNFKVYKNLIQ", "aa_hexdigest": "3c6b481b9c491598c3e290e0cf1544bc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7234.16, "isoelectric_point": 7.961539268493652 }, "id": "LMJBGEANGM_1096", "locus": "GCA013368015_01096" }, { "type": "cds", "sequence": "contig_1", "start": 635148, "stop": 635273, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTCTACTTTTATTAGAGAAAAATTTATTCAACAATCATTTGATGACATAGCTAAAACCAATCAAGATCCTTTTGTTAAAGAATTAATATCTGATTATGAAAAGGCTATGTATAAAGGATGA", "aa": "MPSTFIREKFIQQSFDDIAKTNQDPFVKELISDYEKAMYKG", "aa_hexdigest": "2730798a933e20e8ad170c52ad702dc3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4860.4741, "isoelectric_point": 5.042607688903809 }, "id": "LMJBGEANGM_1097", "locus": "GCA013368015_01097" }, { "type": "cds", "sequence": "contig_1", "start": 635310, "stop": 635426, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTTTTAGATGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTCTAACTGATAAAGAAAGCATAAATGATGTAGAGGAAAATTTCGCTAAAGATCCTAAATGA", "aa": "MRFLDEMDDYLDPDIAFKGLTDKESINDVEENFAKDPK", "aa_hexdigest": "77b08f94d62b2ae5b5aa00c4381596cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4465.874900000001, "isoelectric_point": 4.064465522766113 }, "id": "LMJBGEANGM_1098", "locus": "GCA013368015_01098" }, { "type": "cds", "sequence": "contig_1", "start": 635658, "stop": 635915, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATAAAAATAATCCTAATCCTACCCCAGCGCCAGGTTCAAATCCAGGTTTAATAAATCCAAAACCTCAACCTCAACCAACACCTCCATCTCCCGTTCCTACCCCGGAACCTAAAGCAGAAGAAAAAGAAGGTTATGTTACTTATAAATCAACAAATGAAGATTATTTAAAAAAGAATAACACTTATTATTTTAGCAAATTACTTTATGACCCTGTGGGTTTATCTGATGAGGATTATAACAATCATGAATGA", "aa": "MINKNNPNPTPAPGSNPGLINPKPQPQPTPPSPVPTPEPKAEEKEGYVTYKSTNEDYLKKNNTYYFSKLLYDPVGLSDEDYNNHE", "aa_hexdigest": "9de5b6aabc6888a4f17446a09db9e336", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9532.360099999998, "isoelectric_point": 5.046700096130371 }, "id": "LMJBGEANGM_1099", "locus": "GCA013368015_01099" }, { "type": "cds", "sequence": "contig_1", "start": 636494, "stop": 636811, "strand": "-", "frame": 2, "gene": null, "product": "sugar-phosphate nucleotidyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C", "UniRef:UniRef90_UPI001313E05C" ], "nt": "TTGGGCTATAAAGTAATATATTCGCATATTGATAATGGGCAAGATTTTTATAAATATGAGCCTATTGAAAATTATGATTTAATAATATCAAATCCTCCGTTTAGAAATAAAGCAAATATTATTAAAAGATTGCAAGAACTAAATAAACCGTTTGCTTTAATTTTTGGAGTTCAATGTTTTAATTCTGGCGGTTTTGTAAGCCAATTGCAAAAACTAAAAAATTTAGAGCTTGTGTTCTTAACAAAAAGAATTAAATTTCTTAAAAATTATAAACAAGACCTTAAAAACATTCCTCAGCCTACTTTTCATTCTCTATGA", "aa": "MGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSL", "aa_hexdigest": "3b68e2eb6f24447bacae676d47247993", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 636440, "stop": 636856, "observations": { "mutation": [ 636496 ], "stop": [ 636496 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313E05C", "query_cov": 1.0, "subject_cov": 0.7553956834532374, "identity": 0.99, "score": 214.0, "evalue": 2.72e-70, "gene_start": 1, "gene_end": 105, "reference_start": 17, "reference_end": 121, "reference_sequence": "MPFDTQQSNFVIVLKSLGYKVIYSHIDNGQDFYKYEPIENYDLIISNPPFRNKANIIKRLQELNKPFALIFGVQCFNSGGFVSQLQKLKNLELVFLTKRIKFLKNYKQDLKNIPQPTFHSLWICSGITNKPLSILEGVK" }, "paralog": false, "identity": 0.9855072463768116, "score": 276.0, "evalue": 5e-97, "description": "Internal stop codon at 636496. Nonsense mutation around 636496.", "psc": { "uniref90_id": "UniRef90_UPI001313E05C", "product": "sugar-phosphate nucleotidyltransferase", "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313E05C", "UniRef:UniRef50_UPI001313E05C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313E05C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313E05C" ], "product": "sugar-phosphate nucleotidyltransferase" } }, "id": "LMJBGEANGM_1100", "locus": "GCA013368015_01100" }, { "type": "cds", "sequence": "contig_1", "start": 636970, "stop": 637077, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAACAATTATTCTTTTAATTATCATAATTAGACATTTGAAAAAACGCAATAAATTATTAGCAAATTCTAACCAAATTAATAGCGCTAAAGGAGGTGATAAATAA", "aa": "MITIILLIIIIRHLKKRNKLLANSNQINSAKGGDK", "aa_hexdigest": "c2c0a832c5de1b7bd58e48b167138bda", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3941.776200000002, "isoelectric_point": 11.21964244842529 }, "id": "LMJBGEANGM_1101", "locus": "GCA013368015_01101" }, { "type": "cds", "sequence": "contig_1", "start": 637091, "stop": 637282, "strand": "-", "frame": 2, "gene": null, "product": "DUF1508 domain-containing protein", "db_xrefs": [ "RefSeq:WP_036439043.1", "SO:0001217", "UniParc:UPI000513025C", "UniRef:UniRef100_A0A454C8Y5", "UniRef:UniRef50_A0A9Q9F2Q3", "UniRef:UniRef90_A0A454C8Y5" ], "nt": "GTGTCAAAATACTTTTATAGATTAAGAGGGAGCCAATCTACAGGAAAGAATGCCAAAACATATTCGTTTGGCTCTGCGCAACAATACAATAAAGCATTAATAATGGCTCAAGATGCTATTTCAAAAAATCAATTTAAGGAGGTGCAAATAGAAAGAATACAAGTTATAAAAATTATTAAGCAAAATTCATAG", "aa": "MSKYFYRLRGSQSTGKNAKTYSFGSAQQYNKALIMAQDAISKNQFKEVQIERIQVIKIIKQNS", "aa_hexdigest": "bb61ae58264773743c7974dccf1b1a0d", "start_type": "GTG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000513025C", "ncbi_nrp_id": "WP_036439043.1", "uniref100_id": "UniRef100_A0A454C8Y5", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513025C", "RefSeq:WP_036439043.1", "UniRef:UniRef100_A0A454C8Y5" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C8Y5", "uniref90_id": "UniRef90_A0A454C8Y5" }, "psc": { "uniref90_id": "UniRef90_A0A454C8Y5", "uniref50_id": "UniRef50_A0A9Q9F2Q3" }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2Q3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2Q3" ], "product": "DUF1508 domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_1102", "locus": "GCA013368015_01102" }, { "type": "cds", "sequence": "contig_1", "start": 637291, "stop": 637593, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936841.1", "SO:0001217", "UniParc:UPI0015896B03", "UniRef:UniRef100_UPI0015896B03", "UniRef:UniRef50_A0A6A8PZR1", "UniRef:UniRef90_A0A6A8PZR1" ], "nt": "ATGCATATAGATGAATTTATTGATAAATATCGCAGGAATTTAAAACAATCGGGTTTTAATAAAGACAATATAGACTTCTTTTTATATGGTTTAGAACTAGGATTAAATATAAAAATAAAATCCAGTAGAAATTATTACAACTTTAAAAAATCTATACATTATTCTTCAAATAATTCTCATCCCTCTTCCCTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAGTCATCCCATACCCCCTCGGAATCCCAATCAAAATCAAGAAAGTTATAA", "aa": "MHIDEFIDKYRRNLKQSGFNKDNIDFFLYGLELGLNIKIKSSRNYYNFKKSIHYSSNNSHPSSLSDFDKTPTDLQDSKVINEYNKSSHTPSESQSKSRKL", "aa_hexdigest": "9533f0541c546dadcec9499734919c8d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0015896B03", "ncbi_nrp_id": "WP_175936841.1", "uniref100_id": "UniRef100_UPI0015896B03", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015896B03", "RefSeq:WP_175936841.1", "UniRef:UniRef100_UPI0015896B03" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015896B03", "uniref90_id": "UniRef90_A0A6A8PZR1" }, "psc": { "uniref90_id": "UniRef90_A0A6A8PZR1", "uniref50_id": "UniRef50_A0A6A8PZR1" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZR1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11727.849999999988, "isoelectric_point": 9.300869941711426 }, "id": "LMJBGEANGM_1103", "locus": "GCA013368015_01103" }, { "type": "cds", "sequence": "contig_1", "start": 637584, "stop": 638075, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAATCCGCATTGGCATAGAACAAGACCTTGATATCTCTAAATATACCCAACTAGACCCACAAGGCCATCCAATTTTCGTTGGTAGCCAAATGTCTGAAATTCGTTATGGCCTAAAACAAGGCCTTGTTGTCTCTAAATATACCCAACTAGACCCTCAAGGCCACCCTATATTTGACAGCTTTCAAATGGGTGAAATCCGTAAGGGTCTAAAACAAGGCCTTGATATCTCTAAATATACCCAACTAGACCCTAACGGCCACCCAATCTATGATTTTTGCCAAATGGAACAAATCCGTGAAGGCATAAAACAAGGCCTTGACGTATCTAAACCCACTCAATTAGTCTCTAAATCCTCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAATCCCACTCACAACCTAATCAAAATAAAACCTCAGAATCAAAAAATATAAATAGATGCATATAG", "aa": "MEQIRIGIEQDLDISKYTQLDPQGHPIFVGSQMSEIRYGLKQGLVVSKYTQLDPQGHPIFDSFQMGEIRKGLKQGLDISKYTQLDPNGHPIYDFCQMEQIREGIKQGLDVSKPTQLVSKSSLSDFDKTPTDLQDSKVINEYNKSHSQPNQNKTSESKNINRCI", "aa_hexdigest": "2efa52219f7028b2623fc5fc0096bc47", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18531.64090000001, "isoelectric_point": 6.0690629959106435 }, "id": "LMJBGEANGM_1104", "locus": "GCA013368015_01104" }, { "type": "cds", "sequence": "contig_1", "start": 638178, "stop": 638393, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACAATTTTTGATAATGACCAAATGGAACAAATCTGCCTTGGTCTAGAAGAAGGCCTTGATATCTCTAAATACACCCAATTAAATCATAAAGGTCACCCTATATTTGATAGCTTCCAAATGAATGAAATCCGCGAAGGTCTAAAACACGGCCTTGATGTCTCTCAATATAACAAACTAGACCCTAACGGCCACCCAATCTATGATTGA", "aa": "MKKTIFDNDQMEQICLGLEEGLDISKYTQLNHKGHPIFDSFQMNEIREGLKHGLDVSQYNKLDPNGHPIYD", "aa_hexdigest": "99fc03670438174efefdc08a4cf4a6e9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8249.177599999995, "isoelectric_point": 5.154353141784666 }, "id": "LMJBGEANGM_1105", "locus": "GCA013368015_01105" }, { "type": "cds", "sequence": "contig_1", "start": 638403, "stop": 638597, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193B6F9F" ], "nt": "ATGGAACAAATCCGCAAGGGTCTAAAAAAAGGCCTTGATATCTCTCAATATACTCAATTATCTTCTAAATCATCTTTATCCGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAGTCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MEQIRKGLKKGLDISQYTQLSSKSSLSDFDKTPTDLQDSKVINEYNKSSHTPSESQSKSHSLTK", "aa_hexdigest": "8bbb93071c4397b679a34508918ef634", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00193B6F9F", "query_cov": 0.953125, "subject_cov": 1.0, "identity": 0.836, "score": 91.7, "evalue": 3.03e-23, "valid": false, "uniref50_id": "UniRef50_UPI00193B6F9F" }, "pscc": { "uniref50_id": "UniRef50_UPI00193B6F9F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193B6F9F" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7188.860800000001, "isoelectric_point": 9.005153465270997 }, "id": "LMJBGEANGM_1106", "locus": "GCA013368015_01106" }, { "type": "cds", "sequence": "contig_1", "start": 638796, "stop": 638963, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACTTAATCCGTACAAGTTTAAACCAAGGATTCAATTTATTAAAATATGTAAATACTTCATATAATATAGACCAAATGAAGCAAATTAGTTACGGTCTAAAAAAAGGCCTTGACGTCTCAATTTATGCAAAACAAAACAATTTTAATAGACCTATCTATGATTGA", "aa": "MHLIRTSLNQGFNLLKYVNTSYNIDQMKQISYGLKKGLDVSIYAKQNNFNRPIYD", "aa_hexdigest": "93d05c5cd7fc9babcc10ff4f68e853c3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6470.349900000003, "isoelectric_point": 9.630819129943848 }, "id": "LMJBGEANGM_1107", "locus": "GCA013368015_01107" }, { "type": "cds", "sequence": "contig_1", "start": 639183, "stop": 639467, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3A8E8B", "UniRef:UniRef90_UPI001E3A8E8B" ], "nt": "GTGCCTATAAAAAAGGTTTATACCGAAGAATATATTTTAGATACCTTAGACATGTACAATACAAAATTTCAAATTTACGATTATCTAAAACAAAATAATTTTAATCTTGCTCAAATAAAAACAATTTATTCTAATTATTATGATTACTACAAACATATATTGTTTAGGGATAAAACTTTCGAATATTTAAATAATAAAATACATGTTAATGATTTAAGCGAAATTGCAAAGGATTTTGAAAAGAAACTTGATATTATGGCCTTGCAAAAAGCAGCGGAATCTTGA", "aa": "MPIKKVYTEEYILDTLDMYNTKFQIYDYLKQNNFNLAQIKTIYSNYYDYYKHILFRDKTFEYLNNKIHVNDLSEIAKDFEKKLDIMALQKAAES", "aa_hexdigest": "e032471d2542982d2a86209cb8fff1c7", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 639024, "stop": 639416, "observations": { "mutation": [ 639131, 639185 ], "stop": [ 639131, 639185 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3A8E8B", "query_cov": 0.8085106382978723, "subject_cov": 0.4418604651162791, "identity": 0.895, "score": 134.0, "evalue": 4.5e-38, "gene_start": 18, "gene_end": 93, "reference_start": 1, "reference_end": 76, "reference_sequence": "MQNTKFQIYEYLKQNNFNLNQIETIYSNYHDYYKHILFRDKTFEYLNNKINVNDLREIAKDFEKKLDIMALQKAIEFNKLNPSKISEDEINYRKYWIKQSSDYKNIVQDHFTNKFLNQGLDISKYTQLSSKTSLSDFDKTPTDLQDSKVINEYNKSSHTPSDTQSKSHPLTK" }, "paralog": false, "identity": 0.7615384615384615, "score": 192.0, "evalue": 1.54e-63, "description": "Internal stop codon at 639131, 639185. Nonsense mutation around 639131, 639185.", "psc": { "uniref90_id": "UniRef90_UPI001E3A8E8B", "uniref50_id": "UniRef50_UPI001E3A8E8B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3A8E8B", "UniRef:UniRef50_UPI001E3A8E8B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3A8E8B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3A8E8B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11485.985099999989, "isoelectric_point": 6.074405860900878 }, "id": "LMJBGEANGM_1108", "locus": "GCA013368015_01108" }, { "type": "cds", "sequence": "contig_1", "start": 639799, "stop": 640098, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAATGAAGAATATGTTTATGTAAATCCTTATTTGGAAATAGAGCCAATTAATATTAAAGAAATTAAAGATGATTACATAGAGGCATTAAAAACTTTTGATTTAGATGACCTTTTAGAATCAAGAGATAAAAAATCATTAATTAACTTAGATACTAACAAATCTTCTAAATCATCTTTATCTGATTTTGATAAGACCCCAACCGATCTTCAAGATTCCAAAGTAATCAACGAATATAACAAGTCATCCCATACCCCCTCGGAATCCCAATCAAAATCCCATTCATTAACAAAATAA", "aa": "MPNEEYVYVNPYLEIEPINIKEIKDDYIEALKTFDLDDLLESRDKKSLINLDTNKSSKSSLSDFDKTPTDLQDSKVINEYNKSSHTPSESQSKSHSLTK", "aa_hexdigest": "a24c2fe3e148542075a321749a52e055", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11401.429799999987, "isoelectric_point": 4.7240819931030265 }, "id": "LMJBGEANGM_1109", "locus": "GCA013368015_01109" }, { "type": "cds", "sequence": "contig_1", "start": 640560, "stop": 640748, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6", "UniRef:UniRef90_UPI00158CD6B8" ], "nt": "ATGGAAGCAGATTTATGGGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATGATATTGAATTCGTGGGCATTGACGCCTATGGCAGAGCCTTCGTCATAAGCAGAGAAGATGCAGAAAAATATTATGCAAAGTACTATTTAAAACAAAACAAGTTAGTTGAAAAAGGAGATAAATAA", "aa": "MEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFVISREDAEKYYAKYYLKQNKLVEKGDK", "aa_hexdigest": "b5b290dcb3b0d22f7921df03d005faee", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 640560, "stop": 640961, "observations": { "mutation": [ 640784, 640895 ], "stop": [ 640784, 640895 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158CD6B8", "query_cov": 1.0, "subject_cov": 0.46616541353383456, "identity": 1.0, "score": 128.0, "evalue": 5.05e-37, "gene_start": 1, "gene_end": 62, "reference_start": 72, "reference_end": 133, "reference_sequence": "MTKDKYCAFCGKKIVGMYVKNFWSDFCSREFRAKSEKLQEDLNYWKFHEPQDVYPLNDAWELADKELRKYGMEADLWAFDINNIYEMQNDFYDIEFVGIDAYGRAFVISREDAEKYYAKYYLKQNKLVEKGDK" }, "paralog": false, "identity": 0.9849624060150376, "score": 275.0, "evalue": 2.46e-97, "description": "Internal stop codon at 640784, 640895. Nonsense mutation around 640784, 640895.", "psc": { "uniref90_id": "UniRef90_UPI00158CD6B8", "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158CD6B8", "UniRef:UniRef50_A0A6A8PZW6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7459.247, "isoelectric_point": 4.464725685119628 }, "id": "LMJBGEANGM_1110", "locus": "GCA013368015_01110" }, { "type": "cds", "sequence": "contig_1", "start": 640963, "stop": 641166, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00145.23" ], "nt": "ATGATTATAGATAATAAACCATATTATTTAAGAGTTATTACACCATTAGAAGCAATGCTATTAATGGGATATGATCAACAAGATTATGAAAGAATTAAGAATTTCTGTGATAGTCAAATTTATAAGTTTGCTGGAAATTCTATTGTTGTTAATGTATTGCAAGAAATAATTAAACACATTTTTAAATTAACCAAGGAGGATTAA", "aa": "MIIDNKPYYLRVITPLEAMLLMGYDQQDYERIKNFCDSQIYKFAGNSIVVNVLQEIIKHIFKLTKED", "aa_hexdigest": "01ba26a82d089efa3d2948985b1b2ca8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00145.23", "name": "DNA_methylase", "length": 57, "aa_cov": 0.8507462686567164, "hmm_cov": 0.9473684210526315, "evalue": 7.436793941318637e-11, "score": 37.5190315246582, "start": 6, "stop": 62 } ], "seq_stats": { "molecular_weight": 7972.237499999997, "isoelectric_point": 5.697051811218262 }, "id": "LMJBGEANGM_1111", "locus": "GCA013368015_01111" }, { "type": "cds", "sequence": "contig_1", "start": 641605, "stop": 641970, "strand": "-", "frame": 3, "gene": null, "product": "DNA cytosine methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67", "UniRef:UniRef90_UPI0034A1F568" ], "nt": "ATGAATATAAATAAAATAAAAGTTGTTGAATTATTTGGTGGAATAGGTGCTATTAGAAAAGCTCTAATTAGAACCATCAAACCTCCTAATTTTGAAATTATTGATTATGTCGAAAATGATAAAAAAACAGTAAATGCTTATAATGCAATATATAACGATTTTTACAAACCTTTAGATATAAGAGATTATTCACTTCCTAATAATGTTAATGTTGATTTATTGTTTCACGGATCTCCTTGTCAAGATTTTTCGTGTGCAGGTAAAAATATAGGAGGAAGTAAAGAAAGTAAAAGTCGAAGTAGTTTATTGTGGGAAACAATTAGAATCATTAAAGAAGCTCAAATAAAACCCAAAATTATTATTTGA", "aa": "MNINKIKVVELFGGIGAIRKALIRTIKPPNFEIIDYVENDKKTVNAYNAIYNDFYKPLDIRDYSLPNNVNVDLLFHGSPCQDFSCAGKNIGGSKESKSRSSLLWETIRIIKEAQIKPKIII", "aa_hexdigest": "7a0ac77b447e29b6957d00094f26603a", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0034A1F568", "query_cov": 0.8925619834710744, "subject_cov": 0.9230769230769231, "identity": 0.963, "score": 217.0, "evalue": 3.11e-71, "valid": true, "product": "DNA cytosine methyltransferase", "uniref50_id": "UniRef50_A0A6N2ZW67" }, "pscc": { "uniref50_id": "UniRef50_A0A6N2ZW67", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6N2ZW67" ], "product": "DNA (cytosine-5-)-methyltransferase" }, "genes": [], "id": "LMJBGEANGM_1112", "locus": "GCA013368015_01112" }, { "type": "cds", "sequence": "contig_1", "start": 642249, "stop": 643127, "strand": "-", "frame": 1, "gene": null, "product": "type IV secretory system conjugative DNA transfer family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC", "UniRef:UniRef90_UPI000AF33FFC" ], "nt": "ATGGTTTTAAGGGACACAGAAAGTTTCAATTTAGTTGATATAGTGTCTGATCCTAAACCTTTTGCTATATTTATACATTATCCTGACCATAAACCTACATCTCACTTTTTAGTAAGTATGTTAGTAGAACAAATTTATCAATCGTTAATTGAACAAGCAAACAAAAATGATAACCTAAAATTAGATAGAAAGGCTTTCTTTCTATTGGATGAATTCGGAAATTTACCTGTTGTTGTAAATTTTGATAATAAACTTACAATTTGCCGTTCAAGAAATATTTTCTTTATGTTAGTGGTACAAGATTACAATCAAATTAAGAAATATAACATATATGGAGAAGGAACAGCCCAAGTAATTAAAAACTCTTGCCAAGTAGTATATTTTTTAGCCTCTAATGACGAGCAAACATTAAACGATATTTCAAAAGCGCTCGGAACAAAAGAGGTTAAATCAAAAAGTTATACAACAAGTGATAACCATTCAAGTTCTACTGAAAGTGAAAAATCCAAACCCGTTATGGAAGTAGCAGAACTTAAATCTAAAAAACCTGATGATTTAATAATTATAAAAAATGGTTATAAACCTTTATTAATCAAAACCTTATTAGCTTATAAATATTTCCCAAATGATAATTATGTTTATACCAGAAACGAAAGTAAGCTACTAACTAATGCGGATTTTGAATTATTTGATATTAGAAGTTTAGAAAAACTTATTAAATTTGATAATTCTAAACAAAAAAAACAAAATAACAAAAACCCAAAAGTTTTAGATGATAACAATTTAACTTATTTAGAAGATGTTGAAGATCCTGAGGATTTATCCCGAGAACAAAAAATTCAAAGAATGAAGATGGTTTTTGGAAAATTAGATCTTTGA", "aa": "MVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKFDNSKQKKQNNKNPKVLDDNNLTYLEDVEDPEDLSREQKIQRMKMVFGKLDL", "aa_hexdigest": "098648f50323d25d9a3f823812ca056a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 641982, "stop": 643427, "observations": { "mutation": [ 642098, 642149, 642251, 643247, 643295, 643322 ], "stop": [ 642098, 642149, 642251, 643247, 643295, 643322 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AF33FFC", "query_cov": 1.0, "subject_cov": 0.5983606557377049, "identity": 0.966, "score": 543.0, "evalue": 1.34e-191, "gene_start": 1, "gene_end": 292, "reference_start": 108, "reference_end": 399, "reference_sequence": "QAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHWENDKTISAWILYAKKRALDDNNYYWKKIYNNIEDLSKTPKETFGGILAEAQSVLNVFNNDENLKMVLRDTESFNLVDIVSDPKPFAIFIHYPDHKPTSHFLVSMLVEQIYQSLIEQANKNDNLKLDRKAFFLLDEFGNLPVVVNFDNKLTICRSRNIFFMLVVQDYNQIKKYNIYGEGTAQVIKNSCQVVYFLASNDEQTLNDISKALGTKEVKSKSYTTSDNHSSSTESEKSKPVMEVAELKSKKPDDLIIIKNGYKPLLIKTLLAYKYFPNDNYVYTRNESKLLTNADFELFDIRSLEKLIKLEKSKPKNQDNKNLKVLDDNNLTYSEDVEDPDDLSQEQKIQRMKMVFGKLDLWNNYINLKNDYKRTLIPHKKNQIKEQIEETLSLMWEQYVTNDLESDLLNILWSEAKERKIPNYHDLNVDILLYLLHPTEDISKLIEKSN" }, "paralog": false, "identity": 0.9625779625779626, "score": 908.0, "evalue": 0.0, "description": "Internal stop codon at 642098, 642149, 642251, 643247, 643295, 643322. Nonsense mutation around 642098, 642149, 642251, 643247, 643295, 643322.", "psc": { "uniref90_id": "UniRef90_UPI000AF33FFC", "product": "type IV secretory system conjugative DNA transfer family protein", "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AF33FFC", "UniRef:UniRef50_UPI000AF33FFC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AF33FFC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AF33FFC" ], "product": "type IV secretory system conjugative DNA transfer family protein" } }, "id": "LMJBGEANGM_1113", "locus": "GCA013368015_01113" }, { "type": "cds", "sequence": "contig_1", "start": 643320, "stop": 643460, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32", "UniRef:UniRef90_UPI001E578F32" ], "nt": "ATGTGGATTAGCCAAGCTAAAAAAACATTAATTGTTATTACTAATTTCTTATTGCTATTAGGTATTGATGATAAAAATATTATTCATAAAAATAATTTTAATTTTGAAACAATAGCTTCATTATTAAATACTTCTCACTGA", "aa": "MWISQAKKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSH", "aa_hexdigest": "b1a13d795929f5c842693543b06bcbaf", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 643272, "stop": 643550, "observations": { "insertion": [ 643472 ], "deletion": [ 643441 ], "mutation": [ 643294, 643321, 643484 ], "stop": [ 643294, 643321, 643484 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E578F32", "query_cov": 0.8478260869565217, "subject_cov": 0.40625, "identity": 1.0, "score": 77.4, "evalue": 1.56e-17, "gene_start": 8, "gene_end": 46, "reference_start": 37, "reference_end": 75, "reference_sequence": "MTFEERINKSLEVINNVIESLNWGKALMAICGLAKPKTLIVITNFLLLLGIDDKNIIHKNNFNFETIASLLNTSHRENDKTISAWILYAKKSTWWQ" }, "paralog": false, "identity": 0.9347826086956522, "score": 147.0, "evalue": 6.96e-48, "description": "Internal stop codon at 643294, 643321, 643484. Frameshift due to insertion around 643472. Frameshift due to deletion around 643441. Nonsense mutation around 643294, 643321, 643484.", "psc": { "uniref90_id": "UniRef90_UPI001E578F32", "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E578F32", "UniRef:UniRef50_UPI001E578F32" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E578F32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E578F32" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5284.135999999999, "isoelectric_point": 8.231081581115724 }, "id": "LMJBGEANGM_1114", "locus": "GCA013368015_01114" }, { "type": "cds", "sequence": "contig_1", "start": 643605, "stop": 643772, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCGAATATTGAATTTATGGCACAACTTGATTATTCTAAAAAACCAAATTTAATAATTAGTGATCCAAAGAAAGAAATTATCAAATTTACAGGAGGCTTATTAACTGAAAATGATTACAAAATTTATGTAATTGATTTCTCCGACCCTAAATTATCTCTAAAATGA", "aa": "MPNIEFMAQLDYSKKPNLIISDPKKEIIKFTGGLLTENDYKIYVIDFSDPKLSLK", "aa_hexdigest": "9deac9918115d24a413a97288fdd0f20", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6364.380800000001, "isoelectric_point": 6.098903465270994 }, "id": "LMJBGEANGM_1115", "locus": "GCA013368015_01115" }, { "type": "cds", "sequence": "contig_1", "start": 644130, "stop": 644282, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAACAAGAAAAATTATCAAAATCAAAAATTATAGGAAAGACACTTCTTTCAATAATTGTTAGTACATTTTTATTTTTTGTTCTAACTTTTATTATTAATGTTTTTATGGGCCAGAATAAAAATCCTGGACATTTTAAACTTTTTTGA", "aa": "MEEQEKLSKSKIIGKTLLSIIVSTFLFFVLTFIINVFMGQNKNPGHFKLF", "aa_hexdigest": "ba2bb57e9f1e8664bda2b7f300a10a08", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5777.879399999999, "isoelectric_point": 9.699671363830568 }, "id": "LMJBGEANGM_1116", "locus": "GCA013368015_01116" }, { "type": "cds", "sequence": "contig_1", "start": 644386, "stop": 644790, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8", "UniRef:UniRef90_UPI001313EAF8" ], "nt": "TTGTGCCAAGAATTCATTAAATCTTCAAAGAGTATTTTAGATGAAGTTTTAAACAAAAAAAATATTTCATATTCTCGCATTTCTGATGAAAATAAAAAAAATATTGAAACAATAAAAAAATTCTTATTAAATTATGACAATGATTATTCAAAGCTCTTTAATTCTTATCAAAACCAAATTAATGATTTAAATAATTTAAACATCGAAGATAAATTTCTAAAACAAAGAATTTCAAAAATTATAAATAATGAAATGGATGATTTTCAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAATAATAATGCATTTACTAGCCGAAAGAATTTCGCTTCATTTTTCAATGAATTTCTAAAAATATTTAGCCTAAATAGATCAGAATGA", "aa": "MCQEFIKSSKSILDEVLNKKNISYSRISDENKKNIETIKKFLLNYDNDYSKLFNSYQNQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSE", "aa_hexdigest": "f621a090cf099ffde3b0e4ec39841a80", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 644290, "stop": 644931, "observations": { "mutation": [ 644388, 644868 ], "stop": [ 644388, 644868 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001313EAF8", "query_cov": 1.0, "subject_cov": 0.6291079812206573, "identity": 0.978, "score": 245.0, "evalue": 9.63e-81, "gene_start": 1, "gene_end": 134, "reference_start": 48, "reference_end": 181, "reference_sequence": "MGLSIKYDEDYQKINALTKDIWNTRKQIVGIIANNFANSNEIISKSNLCQEFIKSSKSILDEVLNKKNISYSRISDKNKKNIETIKKFLLNYDNDYSKLFNSYQKQINDLNNLNIEDKFLKQRISKIINNEMDDFQKQVGNKIIKTLLNAYKQNNNAFTSRKNFASFFNEFLKIFSLNRSEWALRNLAEREFLAKDLKELELVEEFYRSKLVH" }, "paralog": false, "identity": 0.9765258215962441, "score": 404.0, "evalue": 4.5e-146, "description": "Internal stop codon at 644388, 644868. Nonsense mutation around 644388, 644868.", "psc": { "uniref90_id": "UniRef90_UPI001313EAF8", "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001313EAF8", "UniRef:UniRef50_UPI001313EAF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001313EAF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001313EAF8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15956.906199999985, "isoelectric_point": 9.361470222473145 }, "id": "LMJBGEANGM_1117", "locus": "GCA013368015_01117" }, { "type": "cds", "sequence": "contig_1", "start": 645319, "stop": 645426, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14", "UniRef:UniRef90_UPI001E466C14" ], "nt": "GTGGTAGATTACATTACAAGACCAGATAAATGCGTTGTATTTAAAGAAGATGAAAATAATTACGATGCTATGAAACTACTTCTTACTAAGGAAGATAAAATAAATTGA", "aa": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKIN", "aa_hexdigest": "4319b445e246145b5e30e86e9d399845", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 645258, "stop": 645426, "observations": { "insertion": [ 645276 ], "mutation": [ 645321 ], "stop": [ 645321 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E466C14", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 1.0, "score": 73.9, "evalue": 7.88e-17, "gene_start": 1, "gene_end": 35, "reference_start": 1, "reference_end": 35, "reference_sequence": "MVDYITRPDKCVVFKEDENNYDAMKLLLTKEDKINWYRQHLKINQNSKQSVFITY" }, "paralog": false, "identity": 0.9636363636363636, "score": 105.0, "evalue": 7.92e-32, "description": "Internal stop codon at 645321. Frameshift due to insertion around 645276. Nonsense mutation around 645321.", "psc": { "uniref90_id": "UniRef90_UPI001E466C14", "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E466C14", "UniRef:UniRef50_UPI001E466C14" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E466C14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E466C14" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4192.786300000001, "isoelectric_point": 4.716124534606934 }, "id": "LMJBGEANGM_1118", "locus": "GCA013368015_01118" }, { "type": "cds", "sequence": "contig_1", "start": 645837, "stop": 646007, "strand": "-", "frame": 1, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013660989", "UniRef:UniRef90_UPI0013660989" ], "nt": "ATGAATAAGGCTGTTGCCAATTATTTTGGAGTTAAATATTTGCAAATGTTTGGTATATTGCATAGTCAAGAAAAAAAGTTTATAACAATAAAAAACGAATTTGGATATTTAAAAATCAAATTTAATGGTTATAAATTTTCTACACAACGTTATTTGTTAATGCAAATTTAA", "aa": "MNKAVANYFGVKYLQMFGILHSQEKKFITIKNEFGYLKIKFNGYKFSTQRYLLMQI", "aa_hexdigest": "619583b08346e36b936d4fe9c2425675", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 645837, "stop": 646157, "observations": { "mutation": [ 646031 ], "stop": [ 646031 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013660989", "query_cov": 1.0, "subject_cov": 0.5283018867924528, "identity": 0.982, "score": 113.0, "evalue": 1.53e-31, "gene_start": 1, "gene_end": 56, "reference_start": 51, "reference_end": 106, "reference_sequence": "LSNAILYAINRHKSKQLNAKKLHCKINSRKVILQNAYYAKFNWSNSKIYLMNKAIANYFGVKYLQMFGILHSQEKKFITIKNEFGYLKIKFNGYKFSTQRYLLMQI" }, "paralog": false, "identity": 0.9622641509433962, "score": 206.0, "evalue": 9.08e-71, "description": "Internal stop codon at 646031. Nonsense mutation around 646031.", "psc": { "uniref90_id": "UniRef90_UPI0013660989", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_UPI0013660989", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013660989", "UniRef:UniRef50_UPI0013660989" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0013660989", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0013660989" ], "product": "MAGa4850 family ICE element protein" } }, "id": "LMJBGEANGM_1119", "locus": "GCA013368015_01119" }, { "type": "cds", "sequence": "contig_1", "start": 646029, "stop": 646628, "strand": "-", "frame": 1, "gene": null, "product": "MAGa4850 family ICE element protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4", "UniRef:UniRef90_A0A454C8Z1" ], "nt": "ATGGGTGCTTATAGTGAATTTATGGTAATGCAGCAAGAGACTAAATTAAACAATGATTATGCCTTTGTGGATGCATATGAAAGAAATTTAAATTGGAGGAGAAATTATAGTAAAAAAATATATGACAAATTAAGCGACATAGCTATAAGCAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATGGTTATTAAATCCTTTAAAAATAAAAAAATCAAAATCATTGATCTAATAAATTTAGGGTTTAAAAAAACTTCTATAAAATCGGCTATAAAAAGACTTATTGAAATAAAAGTTTTAGATGGTGAGTTTTATAAAAAATATAAACTTCTAAAATTAAAAAAAATAGTACTAAAAAACCCTAAGGAAAATTACTGGATTTTAAATGGCTATGATGCCTTAAAAATCTTTCTTCTTAATGGTTTGAGTAATGCTATTTTATATGTAATAAATAGTCATAAATCAAAGCAACTAAATGCTAAAAAATTGCATTGTAAAATAAACTCAAGAAAAGTGATTTTACAAAATGCATATTATGCAAAATTTAACTGA", "aa": "MGAYSEFMVMQQETKLNNDYAFVDAYERNLNWRRNYSKKIYDKLSDIAISKILKSKIVISESYYQKLKTNKVLLEVFMVIKSFKNKKIKIIDLINLGFKKTSIKSAIKRLIEIKVLDGEFYKKYKLLKLKKIVLKNPKENYWILNGYDALKIFLLNGLSNAILYVINSHKSKQLNAKKLHCKINSRKVILQNAYYAKFN", "aa_hexdigest": "82e90832a7e293e8e279c8f15b4fedee", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "both", "pseudogene": { "start": 645837, "stop": 646820, "observations": { "insertion": [ 646718 ], "deletion": [ 646751 ], "mutation": [ 646030 ], "stop": [ 646030, 646739 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C8Z1", "query_cov": 1.0, "subject_cov": 0.6123076923076923, "identity": 0.97, "score": 365.0, "evalue": 1.41e-125, "gene_start": 1, "gene_end": 199, "reference_start": 65, "reference_end": 263, "reference_sequence": "MGLIPNKEKKQDHKGLAFFLSFIKKISLKKRRAQHKHSSSYIYINYTTCSSFFLKNLKIFERNFMGAYSEFMVMQQEAKLNNDYSLVDAYERDLNWRRNYSKKIYDKLSDIAISKILKSKIVISESYYQKLKTNKVLLEVFMVIKSFKNKKIKIIDLINLGFKKTSIKSAIKRLIEIKVLDGEFYKKYKLLKLKKIVLKNPKENYWILNGYDALKIFLLNGLSNAILYAINRHKSKQLNAKKLHCKINSRKVILQNAYYAKFNWSNSKIYLMNKAIANYFGVEYLQMFGILRSQEKKVTTLKLGNLNIKCNVYKFSTQRYLLMQI" }, "paralog": false, "identity": 0.9143730886850153, "score": 565.0, "evalue": 1.08e-206, "description": "Internal stop codon at 646030, 646739. Frameshift due to insertion around 646718. Frameshift due to deletion around 646751. Nonsense mutation around 646030.", "psc": { "uniref90_id": "UniRef90_A0A454C8Z1", "product": "MAGa4850 family ICE element protein", "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C8Z1", "UniRef:UniRef50_A0A9Q9F2D4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2D4" ] } }, "id": "LMJBGEANGM_1120", "locus": "GCA013368015_01120" }, { "type": "cds", "sequence": "contig_1", "start": 646993, "stop": 648069, "strand": "+", "frame": 1, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002359FB52" ], "nt": "ATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGTAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAAGATTCAATTGAGATTGTTAAAAAAGTTTTTAGTGGTAAAGTCGGAAAAGATAAGACAATTACTTACGGAAATATCATTGATAATATAACAGGAAAAATCGTTGATGAGGTATTAGTTAATTGGTTTATTGGTAATAAAAATTATACTGGAGAAAACACCGTTGAAATAAATGCTCATGGTGGAATTGTTGTAACAAACAAAATACTTAATCTATTAATTGCTAATGGTGCAAGGTTGGCAAACAGAGGAGAATTTACAAGGCGAGCATTTTTAAACGGAAAAATTTCGTTAGATAAAGCCGAAGCGATAAATAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATTAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAATCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATCAATGTTGCAATAGTAGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTCTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCTGGAACAACAAGAGACGTGGTAGAAGGTGAATTTCAGATTAATGGTTTTTTATTTAATTTGATTGATACAGCGGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATTGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATAAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATACCAGTAATGAATAAGCAAGATCTTTTAAATAAAGATCAATATCCTAAGAATATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGA", "aa": "MKNNIQDTIVAISSGNVNQAISIIRLSGEDSIEIVKKVFSGKVGKDKTITYGNIIDNITGKIVDEVLVNWFIGNKNYTGENTVEINAHGGIVVTNKILNLLIANGARLANRGEFTRRAFLNGKISLDKAEAINSLIHAKTNKQAEIAISQFNSKDNLIIENLINELLTIISLIEINIDYSDYNDIKEMDANTLTNQLSKFNRKLDSIINKSENACDIYKGINVAIVGRPNTGKSSLLNSLIGSQKAIVTNIPGTTRDVVEGEFQINGFLFNLIDTAGIRQTKDLVESIGIERSKNAIKEAKIVIHMHEPFIKENEEDKIIKELSKGKVYIPVMNKQDLLNKDQYPKNMVLISSKENSL", "aa_hexdigest": "164438ff7228c2700278b0aa2db3d446", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002359FB52", "query_cov": 0.9888268156424581, "subject_cov": 0.9915966386554622, "identity": 0.573, "score": 378.0, "evalue": 1.9e-127, "valid": false, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_UPI002359FB52", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002359FB52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002359FB52" ], "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE" }, "genes": [ "mnmE" ], "id": "LMJBGEANGM_1121", "locus": "GCA013368015_01121" }, { "type": "cds", "sequence": "contig_1", "start": 648100, "stop": 648252, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAACTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCACTAGAATCACTAAGTCAAGGTTTAGGCCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGA", "aa": "MNIDLDDPQIIFNNRKLSLLKQAKISINTALESLSQGLGPEVVILDINEA", "aa_hexdigest": "2500abab43290ee70e2b4b9032c79911", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEF2", "query_cov": 1.0, "subject_cov": 0.9090909090909091, "identity": 0.62, "score": 69.7, "evalue": 7.25e-15, "valid": false, "product": "Catalytic cysteine-containing of GTPase, MnmE family protein", "uniref50_id": "UniRef50_A0A3B0P0P4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5506.285699999999, "isoelectric_point": 4.385492134094237 }, "id": "LMJBGEANGM_1122", "locus": "GCA013368015_01122" }, { "type": "cds", "sequence": "contig_1", "start": 648341, "stop": 648895, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01938.26" ], "nt": "ATGTATAAAATAAATGACAAAATAGTTGTAAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTAAGAAGTGATAAACAAACACTTTTTGTTGAAAATCTCTTATCTGGAGAAGAAGCAGAAGTAGCCATTTATAAATCAAATTCAAAAATTGCTTATGGACGTACAGTTAATTTGATAAAAAAATCAAGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTGTTTGAAAGTGGTGCAGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTCAAAACATTGAACAAATTCAACCTAGTCCAAAAATATTAAATTACAGAAATAAAATAACCTTACATGTGAATTATAAAAACAATAATATTTATATTGGCTTTTATAAAAAAAACAGCAACAAACTAGTTATTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAAGAACTTATTAGTGCCCTAAAACAACAAATTTTATTAAAAGATAATTGA", "aa": "MYKINDKIVVKATELSYEGYGVVRSDKQTLFVENLLSGEEAEVAIYKSNSKIAYGRTVNLIKKSSKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFQNIEQIQPSPKILNYRNKITLHVNYKNNNIYIGFYKKNSNKLVIQNSLPLCHDELDKYYKELISALKQQILLKDN", "aa_hexdigest": "853721fa390db3e2c1d7cb36fa452813", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01938.26", "name": "TRAM", "length": 51, "aa_cov": 0.2608695652173913, "hmm_cov": 1.0, "evalue": 1.6810443067717794e-06, "score": 23.410593032836914, "start": 10, "stop": 57 } ], "seq_stats": { "molecular_weight": 21311.362500000025, "isoelectric_point": 9.256709098815914 }, "id": "LMJBGEANGM_1123", "locus": "GCA013368015_01123" }, { "type": "cds", "sequence": "contig_1", "start": 649004, "stop": 649672, "strand": "+", "frame": 2, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "COG:COG2265", "COG:J", "EC:2.1.1.190", "GO:0008649", "KEGG:K03215", "SO:0001217", "UniRef:UniRef50_D1J8P5", "UniRef:UniRef90_D1J8P5" ], "nt": "TTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAATATATTAAAGATTCTAAAGATATATTCACTTCTTTGTATAAATCAATGCCACAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCAACAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCAGCATGTGAGTGTGCAAAAATTAACATAAACCAAAATTTGATTAATCCAAAACTAGCGGATATAACAAATAGTTCAGCACAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCGATAGTTATATTTGACCCACCAAGAACGGGTTTAACAAATAAAGAAATTGATATGCTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGAATCGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAAATGTTGTCACTTTTGAAAAAATAA", "aa": "MKNNLIFLLKPNVISIQCQYIKDSKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSTKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKININQNLINPKLADITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYRIERLIPYDMFPQTYHIEMLSLLKK", "aa_hexdigest": "e9839ab499227c10f9a34d95b03a23ac", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmD" ], "truncated": "5-prime", "pseudogene": { "start": 648704, "stop": 649672, "observations": { "mutation": [ 648893, 648941 ], "stop": [ 648893, 648941 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8P5", "query_cov": 1.0, "subject_cov": 0.4134078212290503, "identity": 0.973, "score": 434.0, "evalue": 3.84e-149, "gene_start": 1, "gene_end": 222, "reference_start": 222, "reference_end": 443, "reference_sequence": "MYKINDKIVVKATELSYEGYGVVRSDKQTLFVENLLPREEAEVAIYKSNSKIAYGRTVNLIKKSCKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFQNIEQIQPSPKILNYRNKITLHVNYKNNNIYIGFYKKNSNKLVIQNSLPLCHDELDKYYKELISALKQQILSKDNWIKELKLRQITLRHSNWSHNIEVIFIIENPNKKGFDLLKNNLIFLLKPNVISIQCQYIKESKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSTKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKININQNLINPKLADITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYRIERLIPYDMFPQTYHIETVALLSKLDVDKHINIEIELDELDLTSAESKATYAQIKEYVWNKFELKVPTLYIAQIKRKCGIELREHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE" }, "paralog": false, "identity": 0.9751552795031055, "score": 632.0, "evalue": 2.77e-229, "description": "Internal stop codon at 648893, 648941. Nonsense mutation around 648893, 648941.", "psc": { "uniref90_id": "UniRef90_D1J8P5", "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_D1J8P5", "cog_id": "COG2265", "cog_category": "J", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8P5", "EC:2.1.1.190", "UniRef:UniRef50_D1J8P5", "COG:COG2265", "COG:J", "KEGG:K03215", "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8P5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8P5" ], "product": "TRAM domain-containing protein" } }, "id": "LMJBGEANGM_1124", "locus": "GCA013368015_01124" }, { "type": "cds", "sequence": "contig_1", "start": 649940, "stop": 650218, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATCGCCTTGGGTTATGTATCAAGCGCAATTGTTGCAAACGAAATAGGGAAACAAAATTTTGAAGCTGCTCATGAACTTGGAAATAGTGCATTAAAATTTGGCTTCTATGCACAGTCATTATTTTCAATATTAGGAGTTTCATTAACCTGGCCTTTGCTAAGACTTTATTCAATAGATATTCAAATAATTAATTCTGTTGGGTATGGTGTTATGGGAATTATGATGCTAAGAGCTATTCTGGATATAGGAAACCTTACAACCCTAAGGGCTTTATGA", "aa": "MIALGYVSSAIVANEIGKQNFEAAHELGNSALKFGFYAQSLFSILGVSLTWPLLRLYSIDIQIINSVGYGVMGIMMLRAILDIGNLTTLRAL", "aa_hexdigest": "2f6f709c32b0b2cecc21e7188136bfd2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9979.65869999999, "isoelectric_point": 6.526844215393066 }, "id": "LMJBGEANGM_1125", "locus": "GCA013368015_01125" }, { "type": "cds", "sequence": "contig_1", "start": 650331, "stop": 650447, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCAAATTCCTGTTTCAAAAGCAATAGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTGTTTCATGTTTCTTTAATTGAAATTTTATTGTTTTTGGTTATAAAGTGA", "aa": "MNQIPVSKAIGRPNLRMFLTYFVFHVSLIEILLFLVIK", "aa_hexdigest": "701afebf27eef300a3017b460235aed9", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4462.455099999998, "isoelectric_point": 10.277372550964351 }, "id": "LMJBGEANGM_1126", "locus": "GCA013368015_01126" }, { "type": "cds", "sequence": "contig_1", "start": 650567, "stop": 650851, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATCACAACATCACTATAAATGATTTAGCTAGAATGAATCAATTTAATAAGGTGCTATACGGTGGAACTATAAATGGAATTACCCTAGTTAATACAAATCCTTTTAAGGTTTCAGTAACTTCCGAAACAACCATAGCTATAAAATTCTTAAGAATAACCACTTGCGATATATTCATATATTCAATAGCTGTTATTTTTACAGCCAGTTTACAATCTATTGAAAAAAATAAATTTTCAATATTTGGATTAATTACCAGCATATTTGTACGTGCAAGTTGA", "aa": "MKNHNITINDLARMNQFNKVLYGGTINGITLVNTNPFKVSVTSETTIAIKFLRITTCDIFIYSIAVIFTASLQSIEKNKFSIFGLITSIFVRAS", "aa_hexdigest": "b47a869caa80a9c1ac2df0ab7a3ca121", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10494.16859999999, "isoelectric_point": 9.80527057647705 }, "id": "LMJBGEANGM_1127", "locus": "GCA013368015_01127" }, { "type": "cds", "sequence": "contig_1", "start": 650897, "stop": 651376, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAGAAAGAAAACAGTAAACAAAGAAATACAAATCGTTTAAGTATTTTTATTGAAAAATCAAATGAATTATTTAAAGTTAAGCACTTTAAGTATATTTTCAAATTGACTTTACCAATCTTTTTTCAAACACTTTTTGTTGCTTTGGTATCGGTAGTTTCTTCTCTTGCTTCTTCGCATTATATAAAAGTCTATCACGCCGATGGTTCATACAATGGTTTTTATTTTTATATGATTGCAAAAATATTAACTGTTTACAAAATGATAACCTTTATTCCTATTATTTACCAAAGCGGAACATTAGTCTTATGCTCAAATCTTTTTGGACAAAGCAAAATAAGAGACATACCAAAAGCTTTGTGGTCTGCCGTTTATGTATCATTAATAATAAATACTTGTGTCTATTTTATTATGTTTGGACTTGCACCAATAGTTTTACAAGCTTCTGGAACAAAAAACATTCCTCTTTTTGGTTGA", "aa": "MRKKENSKQRNTNRLSIFIEKSNELFKVKHFKYIFKLTLPIFFQTLFVALVSVVSSLASSHYIKVYHADGSYNGFYFYMIAKILTVYKMITFIPIIYQSGTLVLCSNLFGQSKIRDIPKALWSAVYVSLIINTCVYFIMFGLAPIVLQASGTKNIPLFG", "aa_hexdigest": "b2ce13bab3beb987c3729d182f651e72", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18234.496099999993, "isoelectric_point": 9.869481086730957 }, "id": "LMJBGEANGM_1128", "locus": "GCA013368015_01128" }, { "type": "cds", "sequence": "contig_1", "start": 651668, "stop": 652060, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "TTGCAAAACAAGCACAATAAAATTGACGAAGAATTTTTTGAAGATTTAGAAGAAATGCTGATTATGTCTGATATTTCAATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAACGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGATAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATAATAGGTTAAATGTAATATTGGTTGTGGGTGTCAATGGTTCAGGAAAAACTACGTCAATATCAAAAATTGCTAAGAAATTAATTGACGAGGGTAAAAAAGTGTTGATTGCTGCAGGTGATACATTTAGAGCAGCGGCAGTTGAACAATTAGAGATTTGA", "aa": "MQNKHNKIDEEFFEDLEEMLIMSDISIKLVQIIINECKKEVRNENITDPKLINEIIADKLFTIYTSNSVVDTTLNIKDNRLNVILVVGVNGSGKTTSISKIAKKLIDEGKKVLIAAGDTFRAAAVEQLEI", "aa_hexdigest": "32b482f41e01095d3ff1d7245a548d38", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.36923076923076925, "hmm_cov": 1.0, "evalue": 1.7309696207629795e-20, "score": 69.00234985351562, "start": 82, "stop": 129 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 62, "aa_cov": 0.43846153846153846, "hmm_cov": 1.0, "evalue": 5.427153266569074e-15, "score": 50.97073745727539, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14575.721299999977, "isoelectric_point": 5.072220802307128 }, "id": "LMJBGEANGM_1129", "locus": "GCA013368015_01129" }, { "type": "cds", "sequence": "contig_1", "start": 652106, "stop": 652531, "strand": "+", "frame": 2, "gene": "ftsY", "product": "Signal recognition particle-docking protein FtsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "nt": "GTGGATCCAGCGGCTGTTGTTTATAGAGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCAGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATTAATAGGGTTTTAGCCTCTAAAATTCCCGATGCGCCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCTAGGGTATTCAAGGAATCTACACCATTGACTGGAATCATTCTAACAAAAATGGATGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGCGAAAAAGTTGAAGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTAACAAAAGGTATTAACGAATAA", "aa": "MDPAAVVYRAIDIAKSKKYDILIVDTAGRLQNKVNLMNELAKINRVLASKIPDAPHESLLVLDATTGQNGVIQARVFKESTPLTGIILTKMDGTSKGGIVLTIKDEIGLFVKYIGLGEKVEDLAEFDLDSYIYGLTKGINE", "aa_hexdigest": "8f742c1b615280eef70971cd0e04ebab", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH26", "query_cov": 0.9361702127659575, "subject_cov": 0.8407643312101911, "identity": 0.6970000000000001, "score": 189.0, "evalue": 1.68e-59, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_A0A449AH26" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "product": "Signal recognition particle-docking protein FtsY" }, "genes": [ "ftsY" ], "id": "LMJBGEANGM_1130", "locus": "GCA013368015_01130" }, { "type": "cds", "sequence": "contig_1", "start": 652531, "stop": 652755, "strand": "+", "frame": 1, "gene": "ylxM", "product": "putative DNA-binding protein YlxM, UPF0122 family", "db_xrefs": [ "COG:COG2739", "COG:K", "KEGG:K09787", "RefSeq:WP_020002601.1", "SO:0001217", "UniParc:UPI00031E45B5", "UniRef:UniRef100_A0A2K9YTY8", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_A0A2K9YTY8" ], "nt": "ATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTTTATATGAAGATTTGTCATACGGGGAATTAGCCCAAGAATTAGCAATGTCTAGACCAGGGGCTTACGACGCGGTTAAAAAAGCAAAATTACACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAA", "aa": "METIEERDYYVKLFEKYGDLLPQSQKQALQLLLYEDLSYGELAQELAMSRPGAYDAVKKAKLHLKQIDEKLKQS", "aa_hexdigest": "78c4178b8a69d62122a90a1d2b675034", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00031E45B5", "ncbi_nrp_id": "WP_020002601.1", "uniref100_id": "UniRef100_A0A2K9YTY8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031E45B5", "RefSeq:WP_020002601.1", "UniRef:UniRef100_A0A2K9YTY8" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTY8", "uniref90_id": "UniRef90_A0A2K9YTY8" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YTY8", "gene": "YlxM", "product": "Predicted DNA-binding protein YlxM, UPF0122 family", "uniref50_id": "UniRef50_C4XE46", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [ "ylxM" ], "id": "LMJBGEANGM_1131", "locus": "GCA013368015_01131" }, { "type": "cds", "sequence": "contig_1", "start": 652855, "stop": 653022, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGTTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTAGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTTGCGATTGTTGTCGGTGGCGGTAATTTTTGA", "aa": "MSYKRVLIKLSGEGLANKGKHLAIDYELVEKFANQLKTIISRNIEVAIVVGGGNF", "aa_hexdigest": "6d8fef6658cc5c79d62692c6239603c9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.0342, "isoelectric_point": 9.52193202972412 }, "id": "LMJBGEANGM_1132", "locus": "GCA013368015_01132" }, { "type": "cds", "sequence": "contig_1", "start": 653050, "stop": 653583, "strand": "+", "frame": 1, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGCAAAGGGTTAGAGCAGATTATATTGGTATGTTAGCCACAACAATGAACGCGCTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCAAGCCTAACAATGGATCCAAAGGTTTGTGAAGTTTATATAAATGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTGATTTTTGCAGGAGGAACTGGACGTCCATTTTTTACAACCGATACAGCCTCAACATTATTTGCTAGTGAAATTGATGCAGATGCAATATTGATGGGAAAAAACAACGTTGATGGGGTATATGATAGCGATCCAAAATTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTACTAGAAAAGGAATTGAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAGGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAA", "aa": "MQRVRADYIGMLATTMNALALQSGFERLNLHCRVLSSLTMDPKVCEVYINEKAKKYLRDGEVVIFAGGTGRPFFTTDTASTLFASEIDADAILMGKNNVDGVYDSDPKFNKDAKKFPIITYDELLEKELKVIDQTAASMAKENNIDILIFDINEEDSLIRAIENKIPNTIITREREK", "aa_hexdigest": "9d37546a13ad5001476268f5f5e1a856", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 0.96045197740113, "subject_cov": 1.0, "identity": 0.841, "score": 286.0, "evalue": 6.43e-97, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "LMJBGEANGM_1133", "locus": "GCA013368015_01133" }, { "type": "cds", "sequence": "contig_1", "start": 653583, "stop": 654134, "strand": "+", "frame": 3, "gene": "frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "db_xrefs": [ "COG:COG0233", "COG:J", "RefSeq:WP_044329746.1", "SO:0001217", "UniParc:UPI0005C83D5E", "UniRef:UniRef100_UPI0005C83D5E", "UniRef:UniRef50_B3PLW1", "UniRef:UniRef90_D1J8P9" ], "nt": "ATGGAATTAGACAGTTATCTTTTAGAACTAGATGAAGAAATTAGCAAATCAATTACTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGGGCAAATCCTGTCTTAATCAGTAAAATTAAATTAGAATATTATGGTTCACCAATTTCGGTAGAAGAAATAGCATCTATAACTACATCAGGACCATTACAATTAATTGTTAAGCCATATGATATGAGTGTGCTAAAAAATATTGAAAAAGCAATCATGGATAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATCAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTAACAATATAA", "aa": "MELDSYLLELDEEISKSITNYENQLMKVVIGRANPVLISKIKLEYYGSPISVEEIASITTSGPLQLIVKPYDMSVLKNIEKAIMDNKINVSIINEGHQLRLQYPQMTTEKRVEMTKQLASITEQARIGVRQARQDINKKIKADSDLSEDIQKHYLDNIQKEVDKSIEKINEMSKTKEKELLTI", "aa_hexdigest": "55a096ccefd76ab8d64c2c5e0c6bd8a0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005C83D5E", "ncbi_nrp_id": "WP_044329746.1", "uniref100_id": "UniRef100_UPI0005C83D5E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C83D5E", "RefSeq:WP_044329746.1", "UniRef:UniRef100_UPI0005C83D5E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C83D5E", "uniref90_id": "UniRef90_D1J8P9" }, "psc": { "uniref90_id": "UniRef90_D1J8P9", "gene": "Frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "uniref50_id": "UniRef50_B3PLW1", "cog_id": "COG0233", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_B3PLW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLW1" ], "product": "Ribosome recycling factor" }, "genes": [ "frr" ], "id": "LMJBGEANGM_1134", "locus": "GCA013368015_01134" }, { "type": "cds", "sequence": "contig_1", "start": 654192, "stop": 654398, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACCTAAAAATTAGAGCAAAATCTGCAATTATATTATTTAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGGGTGGCAATTTATATGTTTTTTAGCGCGTGGGCTTTGTATGAAGTTATTTCACACAACCAAAACAAGAGATCTGTTAATTTGGTTATATCCTTACTTTCAACATTAATTTGA", "aa": "MKNLKIRAKSAIILFSILIPLLILTYFVKSLGVAIYMFFSAWALYEVISHNQNKRSVNLVISLLSTLI", "aa_hexdigest": "c0ea46aa06fe47485534de8df666caa2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7742.362199999999, "isoelectric_point": 10.214580345153806 }, "id": "LMJBGEANGM_1135", "locus": "GCA013368015_01135" }, { "type": "cds", "sequence": "contig_1", "start": 654492, "stop": 654728, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAATAATAAAAAACACAATATTTTTTAAATCAAATGCCTGGTCTGGTGCTCCGGGATTAGGTTTAATATTGACATTTTTAATAACAACATTCTTACTTATTGTTGATGTAAAGAGTCAAAAAAATGCTAAGAATTGGTTTAAGAACTATTTCATTACAATATTCGCTGTTATATTTATTCCATCAAGTTTTAAACTAATTTTTATGTATAACGTAATGAGTTTGTATTGA", "aa": "MISIIKNTIFFKSNAWSGAPGLGLILTFLITTFLLIVDVKSQKNAKNWFKNYFITIFAVIFIPSSFKLIFMYNVMSLY", "aa_hexdigest": "a38e83c76f105b5a994ca18e142af3eb", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8994.751499999998, "isoelectric_point": 10.000158500671386 }, "id": "LMJBGEANGM_1136", "locus": "GCA013368015_01136" }, { "type": "cds", "sequence": "contig_1", "start": 654903, "stop": 655145, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATTTTGGAAACTCTGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTGCGGCCGTTTTTGTTCTTCCTGCAATTTCTATTATTGGCGACTTAGTATTTTCAGGAATAAAAAGAGCTCAAAATATTAAAGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGACAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAA", "aa": "MGINFGNSDSIKILINQKQIIAAVFVLPAISIIGDLVFSGIKRAQNIKDFSELIAGHGGIMDRFDSLSFNTVFLTFILMI", "aa_hexdigest": "1530833f4c93681f46c9153a297db1f7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8760.249099999995, "isoelectric_point": 6.520194053649903 }, "id": "LMJBGEANGM_1137", "locus": "GCA013368015_01137" }, { "type": "cds", "sequence": "contig_1", "start": 655248, "stop": 655769, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAGCAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAGCCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTGAAATATAATTATTCATGTATAACTACTTCAACAATTGAAATAGACGAAAAAACATTAACACTTTCCTTGCTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGGTTGAACAATAAAAAAAGCGTTGCAAGCAAAAAATTAGATGGTTGA", "aa": "MLDKTFVQLCKEIKFEFDEQQFYGAKVLSVESNLNEWKFKIAFASHIDIEQYKQFREKLVKRFGKVELILAIETIIRNKQIIVDYLNYLIECNPKKYWPFVKYNYSCITTSTIEIDEKTLTLSLLTNEIYEVFKSNKDLFKEALASIGFDFYNVVFNLGLNNKKSVASKKLDG", "aa_hexdigest": "066bdd68cc72a590683ca23d61317a52", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20375.390200000005, "isoelectric_point": 8.278788185119634 }, "id": "LMJBGEANGM_1138", "locus": "GCA013368015_01138" }, { "type": "cds", "sequence": "contig_1", "start": 655872, "stop": 656942, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGATTTAACTACGGCAAACGAAACGTTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTCATTGAAAGCAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTGTTAAAAAATTTTATGATTCCGAAAACGAAATACCTGATATAAAGGTGGGGGACACGATTATTGTTACCGGAAAATTGAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAGCTTATTGAAGATAATGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAACATGAGTGCTCAAGATGGAATTTCAACGCCCACCGACTATATTAATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCCGAATTTTATAATTATGCCAAAAAGCAAAATGATGTTAAGCCTATATACGGCGCAACATTAAGCACCGTTGATTCAGATAAAAAATTCTTCTACAACTTTAAAAATTTCAACTTAAAAGAAGAAGAATACGTTGTATTTGACCTTGAAACAACAGGATTGAGTCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTGCAAACGAAACGTGAACTATCAGCCTTTACTGTTGAAAGCAGCCACATTAATAATGAAATGTTAAAGAACGGTTATGCACAAGATGAAGGCATTAAAAAGATTTATGAAATATTAAAGGATAAAATTGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTAACAAACTCGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTAGCCTTATCTTATTTTTTAAATCCCGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTATTTATGATACAACTGTTGCCCACCGTGGTGATTACGACGCTAATGTTTTGGCTAAGATTTGA", "aa": "MDLTTANETFEEIKITTKGEVFFIESKVLKNKKILYTLKISDYKEAILVKKFYDSENEIPDIKVGDTIIVTGKLSDDNFTKSKVLIAMGKECFIKTEPLQKLIEDNEDNKRVELAARSNMSAQDGISTPTDYINAAKFYGHKAIALTDLDDAQGFPEFYNYAKKQNDVKPIYGATLSTVDSDKKFFYNFKNFNLKEEEYVVFDLETTGLSPRLDDIIEFGATIIKNNTITQKIQFFLQTKRELSAFTVESSHINNEMLKNGYAQDEGIKKIYEILKDKIAVAHNANFDINFCKEKFNKLGLDNSKIFGLDTLALSYFLNPDEFRHSLGYVAKRFNIIYDTTVAHRGDYDANVLAKI", "aa_hexdigest": "40f6abe68fda84f5abe561b7328d4c2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 143, "aa_cov": 0.3960674157303371, "hmm_cov": 0.8321678321678322, "evalue": 3.919476287738733e-08, "score": 28.944557189941406, "start": 201, "stop": 341 }, { "id": "PF00929.31", "name": "RNase_T", "length": 165, "aa_cov": 0.4410112359550562, "hmm_cov": 0.9878787878787879, "evalue": 1.5390727909317254e-31, "score": 105.65760803222656, "start": 200, "stop": 356 } ], "seq_stats": { "molecular_weight": 40667.7374, "isoelectric_point": 5.4840192794799805 }, "id": "LMJBGEANGM_1139", "locus": "GCA013368015_01139" }, { "type": "cds", "sequence": "contig_1", "start": 656949, "stop": 657212, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGACAAACTTGCAAAAGATTACAATATCTTAAACTCACAGGATTTAAACAATGTCGATAGCCAAAAAATACTTTCTCATAAATTTACATACGAAACAAGAATATTAGTAAAAAATCAAGCTGGCCTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCTCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGCAATGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGA", "aa": "MIDKLAKDYNILNSQDLNNVDSQKILSHKFTYETRILVKNQAGLKKLFKIISRSLTSQFKKNPQIYFDQLGNDEDLFFGSATNQSYV", "aa_hexdigest": "7f2aa703e437c38f42ef82ce7b1a8918", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10093.354599999991, "isoelectric_point": 9.222669792175292 }, "id": "LMJBGEANGM_1140", "locus": "GCA013368015_01140" }, { "type": "cds", "sequence": "contig_1", "start": 657243, "stop": 657821, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAATTCACTAAAATATTATGACTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGTAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCGGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAAATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGATAACTCACCTCAAAAATTAAGAGAAGTTGTTTATAGGACTTTAAAAGAAAGATACGGGGATAATCCTGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAATCCAATTATAAAGTATGGATATTCTGTTATTTATTGA", "aa": "MLNSLKYYDYIELPPISSFQYMIDSNHVTLDELKFLYKDLISKAKSLNKMCVAVSDCRYIYNYQRLIHEIYIEAPTLGGGLHWLKKRPSHPEFRYLTTREMLKEFAFLNDSYLINEIVIENPNKIANEISSNIQVIKDKLYVPTFDNSPQKLREVVYRTLKERYGDNPDPIIVKRIEKELNPIIKYGYSVIY", "aa_hexdigest": "3b2a3943ebcbe6e616d5204578438495", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22758.07350000002, "isoelectric_point": 8.329589271545409 }, "id": "LMJBGEANGM_1141", "locus": "GCA013368015_01141" }, { "type": "cds", "sequence": "contig_1", "start": 658014, "stop": 658292, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D", "PFAM:PF07733.18" ], "nt": "TTGCCAGACAAGCATTGTCCAAATTGCAACTCAATATTGTCAAAAGATGGGCATAATATCCCTTTTGAAACATTCTTGGGATTTGAAGCGGATAAAGTTCCTGATATTGATTTGAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAAGAATTGTTTGGAGAAGCGCATACATATAGAGCTGGTACAATAGCAACCGTAGCAGAAAAAACAGCATATGGATTTTGCAAAAAATATGAAGAAGAAAAGAGAGGAAATTATCAACCTTGA", "aa": "MPDKHCPNCNSILSKDGHNIPFETFLGFEADKVPDIDLNFSGEYQPTIHNYVKELFGEAHTYRAGTIATVAEKTAYGFCKKYEEEKRGNYQP", "aa_hexdigest": "e91ce885ba96cabbf188544f2aa38639", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00311D31FE", "query_cov": 0.8695652173913043, "subject_cov": 0.9523809523809523, "identity": 0.688, "score": 123.0, "evalue": 5.56e-35, "valid": false, "uniref50_id": "UniRef50_UPI0023B0427D" }, "pscc": { "uniref50_id": "UniRef50_UPI0023B0427D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 59, "aa_cov": 0.6413043478260869, "hmm_cov": 1.0, "evalue": 1.3472773482169642e-19, "score": 66.29480743408203, "start": 18, "stop": 76 } ], "seq_stats": { "molecular_weight": 10477.550399999993, "isoelectric_point": 5.471401023864746 }, "id": "LMJBGEANGM_1142", "locus": "GCA013368015_01142" }, { "type": "cds", "sequence": "contig_1", "start": 658299, "stop": 658448, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "TTGTTCTTGGACTTTTTAGCATCTAAAACACAAGGAGTAAAGAGAACAACTGGACAACATCCAGGAGGAATTATTATTATTCCTAAAGAATTCGATGTTGAGGATTTTACGCCTGTAAACTACCCTGCCAACGACACAGAAAGTTCTTGA", "aa": "MFLDFLASKTQGVKRTTGQHPGGIIIIPKEFDVEDFTPVNYPANDTESS", "aa_hexdigest": "b044285cd8dda354983023088a65d810", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 41, "aa_cov": 0.8367346938775511, "hmm_cov": 0.9512195121951219, "evalue": 2.2380745574992915e-09, "score": 32.7967643737793, "start": 6, "stop": 46 } ], "seq_stats": { "molecular_weight": 5412.988600000002, "isoelectric_point": 4.646496772766113 }, "id": "LMJBGEANGM_1143", "locus": "GCA013368015_01143" }, { "type": "cds", "sequence": "contig_1", "start": 658503, "stop": 658784, "strand": "+", "frame": 3, "gene": null, "product": "DNA polymerase III polC-type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "nt": "TTGTTGGGTCATGATGATCCTACCGTTATTAGAATGCTTGAAACATTGACAAATACAAACGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTTTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGGGGCGAAACAACTGGAGCTTATGGGTTGCCTGAATTTGGAACAAACTTTGTAAGAAAAATGTTAAAAAAAGCTCAACCGAGATCGTTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGA", "aa": "MLGHDDPTVIRMLETLTNTNAKEIPKFDAEVMKLFYTTESLKISPSDIGGETTGAYGLPEFGTNFVRKMLKKAQPRSFNDLILLSGLSHGTDV", "aa_hexdigest": "2d0c3d1ead0a0adca86ab0ead46ba3d3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBH3", "query_cov": 1.0, "subject_cov": 0.9117647058823529, "identity": 0.624, "score": 118.0, "evalue": 6.6e-33, "valid": false, "product": "DNA polymerase III alpha subunit finger domain-containing protein", "uniref50_id": "UniRef50_A0A3B0PG80" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "product": "DNA polymerase III polC-type" }, "genes": [], "id": "LMJBGEANGM_1144", "locus": "GCA013368015_01144" }, { "type": "cds", "sequence": "contig_1", "start": 659148, "stop": 659336, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAGTGGCGCAGCAAGCGTTCACCAAAAACTCATTGAATTAAAAAATCGCCAAAATTCAACTGGTGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATATGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCCTTGGCTGATAAATGA", "aa": "MSSGAASVHQKLIELKNRQNSTGEKLSVKEEALIPVLEITQELYARGFKIKNVDLKLSLADK", "aa_hexdigest": "b4b10474809773a2b317a662a22fcc8f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6881.9046, "isoelectric_point": 9.09998645782471 }, "id": "LMJBGEANGM_1145", "locus": "GCA013368015_01145" }, { "type": "cds", "sequence": "contig_1", "start": 659382, "stop": 659561, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "GTGGATGGTTTAGGAATTACTGTTGCTCAAACAATAGTATCTGCAAGAAAAGAAGGCTTTTTCCTTTCAATTGAAGACCTTGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCGGAATTAAAAAAAATAGGCGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAA", "aa": "MDGLGITVAQTIVSARKEGFFLSIEDLASRTKVNSRIISELKKIGVLDCLDDTNQVSLF", "aa_hexdigest": "9f00ef4ff60f338034dae1f1abda16da", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 56, "aa_cov": 0.8813559322033898, "hmm_cov": 1.0, "evalue": 6.643304449295384e-11, "score": 37.870018005371094, "start": 2, "stop": 53 } ], "seq_stats": { "molecular_weight": 6457.3866, "isoelectric_point": 5.086430549621581 }, "id": "LMJBGEANGM_1146", "locus": "GCA013368015_01146" }, { "type": "cds", "sequence": "contig_1", "start": 660224, "stop": 660511, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAGAAATTGGAATTAAAAAGGTTAAAGCTTCAGTGTTAAATACCTTAAATTTAAAAACTAACAATAAAACAATAATAATTTGTATTGCAGCAATTGCTAATGCTATTGGAAACTCAACACCTCATCTTGATAAATTTTTTGTTGATTTTATAATTCAACCATCTATGCCTAAAATTAAAAACATTATAAAAGAAGGAATGACAATTAAAAATAAACCCGTTAGAGCTATTTTTCATGAACGTGACAATTCTCTTTTTGGCTTTCCTGATTCTACAACATAA", "aa": "MAKEIGIKKVKASVLNTLNLKTNNKTIIICIAAIANAIGNSTPHLDKFFVDFIIQPSMPKIKNIIKEGMTIKNKPVRAIFHERDNSLFGFPDSTT", "aa_hexdigest": "b345b622f40de8dde75c05050c3cc955", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10539.367099999989, "isoelectric_point": 9.869610023498534 }, "id": "LMJBGEANGM_1147", "locus": "GCA013368015_01147" }, { "type": "cds", "sequence": "contig_1", "start": 660742, "stop": 661998, "strand": "+", "frame": 1, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "KEGG:K00600", "RefSeq:WP_020002605.1", "SO:0001217", "UniParc:UPI00036E4BF4", "UniRef:UniRef100_UPI00036E4BF4", "UniRef:UniRef50_P56089", "UniRef:UniRef90_D1J8Q3" ], "nt": "ATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGGCACTTATCACACGGATATAAAATTTCATTTAGTGGTAAATTTTACGAAGCTCATACTTATTCTGTTAATGATGAAGGTGTATTGGATTACGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGCGATGAAGTAGGTGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCACCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATCTTTAAAAGAGTTAATAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGCTTTAGAGAAGCATTACAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAACTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAACACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTTTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACCAATTCATTTCCAATTAAACGTTCTCATTTAGTTCGTTAG", "aa": "MYKKISLDDKEIEELINLENQRLEDHVELIASENYVSEDVMKANGSCLTNKYAEGYPNKRYYGGCEYVDKIEEIAQERAKKLFNVKYANVQPYSGSVANAAVYMAMVDPGDKVLGLDLNSGGHLSHGYKISFSGKFYEAHTYSVNDEGVLDYDKILEIAKEVKPKMIICGYSAYSQIVDFAKFRKICDEVGAKLFADISHISGLIIAGLHPSPSGYADVIMTTTHKTLRGTRGAIILTNDEEIFKRVNSAVFPGVQGGPLLHQIAAKAVSFREALQPEFIDYQKQLLLNSKIMCQTFLNKGVKIISGLTQNHLFMIDVKTSYGITGKYATEVLGRVNITVNKNTIPNDSEKPTVASGIRIGSAAMTSRGLKEDEFIILANLIDKVLRDPENENLLYVVKKEIAKLTNSFPIKRSHLVR", "aa_hexdigest": "035b405ad96d1838988f80f7a19acbf7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036E4BF4", "ncbi_nrp_id": "WP_020002605.1", "uniref100_id": "UniRef100_UPI00036E4BF4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036E4BF4", "RefSeq:WP_020002605.1", "UniRef:UniRef100_UPI00036E4BF4" ] }, "ips": { "uniref100_id": "UniRef100_UPI00036E4BF4", "uniref90_id": "UniRef90_D1J8Q3" }, "psc": { "uniref90_id": "UniRef90_D1J8Q3", "gene": "GlyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" }, "genes": [ "glyA" ], "id": "LMJBGEANGM_1148", "locus": "GCA013368015_01148" }, { "type": "cds", "sequence": "contig_1", "start": 662009, "stop": 662305, "strand": "+", "frame": 2, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_012855740.1", "SO:0001217", "UniParc:UPI0001BC24B5", "UniRef:UniRef100_D1J8Q4", "UniRef:UniRef50_A0A3G8LGV6", "UniRef:UniRef90_D1J8Q4" ], "nt": "ATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACCAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAA", "aa": "MILAEIKNLIKSSISIIPGIVDFVELTSTNNINIENDGLIIEEDPQNKNIINITIGLRIMSNLSAKNIVEGIYEIIPYNLTSYDIKLGTLTIYIKGTK", "aa_hexdigest": "7f63b54d2cc3e32b5b5d28cdfbd465c3", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001BC24B5", "ncbi_nrp_id": "WP_012855740.1", "uniref100_id": "UniRef100_D1J8Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC24B5", "RefSeq:WP_012855740.1", "UniRef:UniRef100_D1J8Q4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8Q4", "uniref90_id": "UniRef90_D1J8Q4" }, "psc": { "uniref90_id": "UniRef90_D1J8Q4", "uniref50_id": "UniRef50_A0A3G8LGV6" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGV6" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "LMJBGEANGM_1149", "locus": "GCA013368015_01149" }, { "type": "cds", "sequence": "contig_1", "start": 662434, "stop": 663933, "strand": "+", "frame": 1, "gene": "fakA", "product": "DhaL domain-containing protein", "db_xrefs": [ "COG:COG1461", "COG:I", "SO:0001217", "UniRef:UniRef50_N9TTI7", "UniRef:UniRef90_D1J8Q5" ], "nt": "ATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCTGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCATCTAAAAAGTCTGCTAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGTGTTACTGATTCAGGTGGTGAAGGTTTATATGCAATATTGCAGGGAATGAGCGCATACTTTAGTGGTAATAAAGTAGAACCTATTGTTGCAGAAGAGTCGGTTGAAAAATTTATTTCTGATGAAGAGGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTAGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTGGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTCGTAAAAATTAAAATAGATAACATGACTTTACAAGCAAACAATAGTAAGAAAACTGCAAAGAAAAAACAAACCGAAGCGCCAAGCAATGTTGAATTAAAAAATAGCGCCATTGTTTCATGCAATACAGGCCAAGGGATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGCGGTCAAACAAATAATCCTTCAATTCAAGATATTGTTAATGCAATAAATGCAGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGCACAATTATTAGAGATAAGAATGTAATTATCATTCCAACTAGAAGTCAAGTCCAAGGATTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCACGAATTAATGAGCAATAGTTTAAAACATATAAGATATGGAGAAATTGCTGCCGCTATAAAAGATGCTAAATTAAACGGAATAAAGGTTAAAGCATCCGATTATATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTTTCGGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAATGATGCGCTAGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGTGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAG", "aa": "MSSTISSACNSECLEDDCSIESASRIVAQNMLMSARGNSGVILSQIFKGFSNGFASKKSANAFDLVNAFEMAAKSAYGAVLKPVEGTILTVIREVSEGLKNQVVVDTTIEEVFEIAVKLARKSCDGTPKLLKILKEVGVTDSGGEGLYAILQGMSAYFSGNKVEPIVAEESVEKFISDEEVYEGEFGYCTEFILELYKPKKFDKDYLVRKLEKIGNSMVVVNDDKILKVHIHTPKPGDVLNSVNSLGQFVKIKIDNMTLQANNSKKTAKKKQTEAPSNVELKNSAIVSCNTGQGIVTLMKENGVSYVVDGGQTNNPSIQDIVNAINAVNAKTVFVLPNNSNIILSAQQAGTIIRDKNVIIIPTRSQVQGLNVALNYSSENSDEENHELMSNSLKHIRYGEIAAAIKDAKLNGIKVKASDYMVIVEGKLQKTSKSLFNAAQLLVSELVSDDSQVVTIIYGQDVSENDALELQNYIETTYDLVVEIYQGDQGIYPYLISVE", "aa_hexdigest": "6c8c1bc7fbc509481fd43aaeb7750a7d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J8Q5", "query_cov": 1.0, "subject_cov": 0.9206642066420664, "identity": 0.9940000000000001, "score": 927.0, "evalue": 0.0, "valid": true, "gene": "FakA", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_N9TTI7", "cog_id": "COG1461", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_N9TTI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TTI7" ], "product": "DhaL domain-containing protein" }, "genes": [ "dhaL", "fakA" ], "id": "LMJBGEANGM_1150", "locus": "GCA013368015_01150" }, { "type": "cds", "sequence": "contig_1", "start": 663943, "stop": 664422, "strand": "+", "frame": 1, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0008610", "GO:0043811", "SO:0001217", "UniRef:UniRef50_D1J8Q6", "UniRef:UniRef90_D1J8Q6" ], "nt": "ATGAAAAAAATAGTATTTGATTTATTGAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTGCAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAAAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTGAGAACTCAAAATTCAATGTTGGAAGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGTGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCTATTCTGCCAACAACAACGAATAAGAAAATGTTATTAATAGATGCAGGGGCAAACATTGAAATCAAACCAGAATATTTAAGTCAATGGGCTATGATAGCAAGTGAATGA", "aa": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTNKKMLLIDAGANIEIKPEYLSQWAMIASE", "aa_hexdigest": "2e5b95c3362473ab17203bd9dceff479", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "plsX" ], "truncated": "3-prime", "pseudogene": { "start": 663943, "stop": 664725, "observations": { "mutation": [ 664420, 664708 ], "stop": [ 664420, 664708 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q6", "query_cov": 1.0, "subject_cov": 0.4746268656716418, "identity": 1.0, "score": 306.0, "evalue": 1.41e-102, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTNKKMLLIDAGANIEIKPEYLSQWAMIASEWYSILFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKNWKTKLAALILKRHLREFKQKFDYRNTGGAFIIGLEKIVVKAHGGSDELAFYNALNQIKIGIENNISTILNKKFKEADNEE" }, "paralog": false, "identity": 0.9923076923076923, "score": 496.0, "evalue": 5.81e-180, "description": "Internal stop codon at 664420, 664708. Nonsense mutation around 664420, 664708.", "psc": { "uniref90_id": "UniRef90_D1J8Q6", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_D1J8Q6", "cog_id": "COG0416", "cog_category": "I", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q6", "EC:2.3.1.274", "UniRef:UniRef50_D1J8Q6", "COG:COG0416", "COG:I", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q6" ], "product": "Phosphate acyltransferase" } }, "id": "LMJBGEANGM_1151", "locus": "GCA013368015_01151" }, { "type": "cds", "sequence": "contig_1", "start": 664432, "stop": 664710, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGGAAGCAAAAAGCCAATAGTAGGACAATTAAATGTAGGCGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGTACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGA", "aa": "MFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKN", "aa_hexdigest": "59450b628a35b04801e5ffb8ffc405e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9999.448399999992, "isoelectric_point": 8.892075920104983 }, "id": "LMJBGEANGM_1152", "locus": "GCA013368015_01152" }, { "type": "cds", "sequence": "contig_1", "start": 664940, "stop": 665473, "strand": "+", "frame": 2, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_D1J8Q7", "UniRef:UniRef90_D1J8Q7" ], "nt": "ATGAAGATTAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAAAATTAGATGAACAAAAAAGACGTTATTTTTGTAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTCATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCGACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTCTTATCGAGATATTATGGTTTTCCAAACTGTTTGAGGACAGGCAAAAGTGCATTTGCAAACGGAAAAGGAATTAAATCAGATTCAGATCTAATTGAATCATTCATTGGTGCTTGTTATAAAGCCTGCGGCTTGAAATTTACTAAAGAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGA", "aa": "MKINFEENLQNALLKFGINFQKLDEQKRRYFCNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLKFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSN", "aa_hexdigest": "1c71d49bb302f31405077fedf917ffdd", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rnc" ], "truncated": "3-prime", "pseudogene": { "start": 664940, "stop": 665635, "observations": { "mutation": [ 665471 ], "stop": [ 665471 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q7", "query_cov": 1.0, "subject_cov": 0.7662337662337663, "identity": 0.9890000000000001, "score": 357.0, "evalue": 6.97e-124, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKNNFEENLQNALLKFGINFQKLDEQKRRYFCNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLEFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSNWGCRPRYEDQALNNGKFESKVFINNNLYGEGIGQSKKESQTKAANDALKKMAKI" }, "paralog": false, "identity": 0.9783549783549783, "score": 461.0, "evalue": 1.87e-167, "description": "Internal stop codon at 665471. Nonsense mutation around 665471.", "psc": { "uniref90_id": "UniRef90_D1J8Q7", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_D1J8Q7", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q7", "EC:3.1.26.3", "UniRef:UniRef50_D1J8Q7", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q7" ], "product": "Ribonuclease 3" } }, "id": "LMJBGEANGM_1153", "locus": "GCA013368015_01153" }, { "type": "cds", "sequence": "contig_1", "start": 665657, "stop": 668593, "strand": "+", "frame": 2, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0005524", "GO:0007059", "GO:0030261", "RefSeq:WP_044420802.1", "SO:0001217", "UniParc:UPI0005CA2081", "UniRef:UniRef100_UPI0005CA2081", "UniRef:UniRef50_A0A448ZX91", "UniRef:UniRef90_D1J8Q8" ], "nt": "ATGAAGTTAATTCAAGTTGAAGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCGATAATAGGGCCTAATGGGTCAGGAAAATCAAACATAAATGACGCAATCAGATGGGTGTTGGGTGAAACTAGTTCCAAAGTATTGCGTGGAGATACAATGGAAGACGTTATTTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACAAGGGGAAATGGTTCAAACGAATATTACATTAATGGTGAATTAGCAAGGCAAAGAGACATTAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCAAGTTTAGCAATCATAGGTCAAGGAACTATTTCCAACATTGCCGAATCAACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCTGGAACATCAATGTACAAAAGTAAGAAGATTGAAGCGCAAAGAAAATTAGAAAAGACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAGCAATTAAAAGAAGTCGAAGTTGCACTATTGGTTCATGATTTCATGGATTATTCAGATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATCTAATTAAAGAAGAATTAGAAACTAAAATAAGGGTATACAACGAAGCAAGTGAACAAAAAAGCAAGTTTGTTTTGGAATTAGAAAATAACATTAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACCCTAGAAATAAGAAACGCAAAAGAAGCAAAGCACCGCGAAATGCTTTTGAGTGGCGAACTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTAATATTTTAGGGTATAAGACATTCCTAGAAACTAAAAAGAAGGAATCTGAACAATTATCTCAATCTGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCCTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAGACAAATTTGGCTAAAGGAACACATAATATTGTTGAAAACTCTCATTTATTTAGAGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAAATACGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGAGGTAGAGCAACTTTTATTCCTTTATCATCAATTAAACCAAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCAACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTTTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCTAAATTGCTAGAAACTCGTTACATGGTCGTAACGCTAGATGGCGACATCATAAGAGTTGGTGGAGTTCTTTCAGGAGGACAAGCAAGTCAAAACGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCGAAAATTTCACAATTATCAAATGAGTTGTTTGAATTAGAAAAAGACCAAAATAAAGAAACATCATTATTAACAAATATTTCTCTTGAAAGAGCAAAGATTGACAACTTATATAGAACAACATTGGATAAGTTTGATTCATTACGTGAACAATATCGTAGTGCTTCGCAAGAAGAATATGAGGCAGAAAACAATAAAAAAATTGATATAGTAGCAGCAATTCAACAATTAATGCTTGAAAGAAGTGATTTATCAGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTAATTAAAGATAATGCAAACAAAATAGCAGAAAAAGCAAAGGCAGAAACAATTATTCAAAATGCAAAGCAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTATAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTGGATATAAGAGAGTTAGGACATGTAAACCTAGATTCAATTAATCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCGGCAAAAACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACACAAACAGTTACATTAGTCAATGCTGAATTCAAGCATGTGTTTGCAAAAATGTTTGGCGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGCGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGACGAAGTTGAGGCAGCCCTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTGCACACTCTAAAAAAAGATACCCAATTTATAGTGATTACTCACCGCCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGTGCAACTATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGATGCAAAGAAAACTATTGAAAACAACAATTAA", "aa": "MKLIQVEAHGFKSFADKVTLKFDGGIVAIIGPNGSGKSNINDAIRWVLGETSSKVLRGDTMEDVIFSGSKTEKEMDRAEVILTFDNKDRSCDIPYDVFTISRVLTRGNGSNEYYINGELARQRDIKEIAMQSGISKSSLAIIGQGTISNIAESTPEKRREIFEDAAGTSMYKSKKIEAQRKLEKTQEALEQISILIQEQERQLKPLQRQAEKAKIYKTKAEQLKEVEVALLVHDFMDYSDKLEKLNVEGQDYNLIKEELETKIRVYNEASEQKSKFVLELENNIKKISERLSEISEEITTLEIRNAKEAKHREMLLSGELTISPKEKLAAMKEELNALNTNILGYKTFLETKKKESEQLSQSASIRSTKINDIKRELSFKKEELNKIKSKLDVIEEIKSKKTNLAKGTHNIVENSHLFRGYKALVSELIEVNEKYALAIETILSSAMQHIVVDKPETAVEAINFLKSNNGGRATFIPLSSIKPKFVPEQHIVVAQTQKGFLGIASELVSTEEQYNVLKKFLLGNVLVADNIENANKLSKLLETRYMVVTLDGDIIRVGGVLSGGQASQNVSTLAADEQVKKLQDLIPVINAKISQLSNELFELEKDQNKETSLLTNISLERAKIDNLYRTTLDKFDSLREQYRSASQEEYEAENNKKIDIVAAIQQLMLERSDLSAKKSSQEDIYHNLKQELQNLTNQRFQVSEELNNLIKDNANKIAEKAKAETIIQNAKQRLSEQYGMLFEVAKQFYNPDIDFEVARKTVADLKLDIRELGHVNLDSINQLEELETRYKEVKSQEQEIISAKTTIEDAISEMDKIIIERMTQTVTLVNAEFKHVFAKMFGGGMAEIRYTDPENILETGIDVIAQPPGKSIKNLKLFSGGEKALIAISLLFSILKAKPLPLCILDEVEAALDEANVIRFAEYLHTLKKDTQFIVITHRQGTMERVNKLYGATMQKRGVTTFFAVNLSDAKKTIENNN", "aa_hexdigest": "dd5b17cc858c6b6c08383d3b2332d145", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0005CA2081", "ncbi_nrp_id": "WP_044420802.1", "uniref100_id": "UniRef100_UPI0005CA2081", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CA2081", "RefSeq:WP_044420802.1", "UniRef:UniRef100_UPI0005CA2081" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CA2081", "uniref90_id": "UniRef90_D1J8Q8" }, "psc": { "uniref90_id": "UniRef90_D1J8Q8", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_A0A448ZX91", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZX91", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZX91" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "LMJBGEANGM_1154", "locus": "GCA013368015_01154" }, { "type": "cds", "sequence": "contig_1", "start": 668828, "stop": 669832, "strand": "+", "frame": 2, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175936866.1", "SO:0001217", "UniParc:UPI00158DF07F", "UniRef:UniRef100_UPI00158DF07F", "UniRef:UniRef50_UPI00158DF07F", "UniRef:UniRef90_UPI00158DF07F" ], "nt": "ATGAAAAAGTCTAAGAAAATATTATTAACAATAGGAATTGCATTTCCACTTTTAGCTTCAACTCCATTAGTTGCTGCTGGATGTGTAGAAACAAAGAAGCCAGAAGGTGAAAAACCAGGAACAACTCCTGAAGTTAAGCCTGAAGGTGAAACCACAATAGAAAATATCATTAATAAACATAAAGAAATTAAAGATGATGAATTAAACAATACTAAATCATTATTAGATCAATTTAAAAAATCAATTGAAGAAAGAACAAATCAAGCAGCATTAAACGAAGCAAAGAAACAATTAAATTCAGCTTTAAAACATGCAGCAAAAATAAACACAGGGTCGTTTGATTTTAACCAAAAATTTAGTTTTAATAAAGAATATGTGAAGGGATTAGAAGCAATTAAAGGTAACGATTTGAATGCTATTAAAGAAATTACATTTAAATTAAATAGTTTTATAAATTTAGCGACATCTAAAAAACCAGGAACAACTCCTGAAGTTAAGCCAGAAGGTGAAACCACAGCAGCATTAAACGAAGCAAAGAAACAATTAAATTCAGCTTTAAAACATGCAGCAAAAATAAACACAGGGTCGTTTGATTTTAACCAAAAATTTAGTTTTAATAAAGAATATGTGAAGGGATTAGAAGCAATTAAAGGTAACGATTTGAATGCTATTAAAGAAATTACATTCAAATTAAATAGTTTTATAAATTTAGCGACATCTAAAAAACCAGGAACAACTCCTGAAGTTAAGCCAGAAGGTGAAACCACAGCAGCATTAAACGAAGCAAAGAAACAATTAAATTCAGCTTTAAAACATGCAGCAAAAATAAACACAGGGTCGTTTGATTTTAACCAAAAATTTAGTTTTAATAAAGAATATGTGAAGGGATTAGAAGCAATTAAAGGTAACGATTTGAATGCTATTAAAGAAATTACATTCAAATTAAATAGTTTTACCTATAAATTTAGCAACATCACAAAAACCAAAAACAGCAATAAAAATTAA", "aa": "MKKSKKILLTIGIAFPLLASTPLVAAGCVETKKPEGEKPGTTPEVKPEGETTIENIINKHKEIKDDELNNTKSLLDQFKKSIEERTNQAALNEAKKQLNSALKHAAKINTGSFDFNQKFSFNKEYVKGLEAIKGNDLNAIKEITFKLNSFINLATSKKPGTTPEVKPEGETTAALNEAKKQLNSALKHAAKINTGSFDFNQKFSFNKEYVKGLEAIKGNDLNAIKEITFKLNSFINLATSKKPGTTPEVKPEGETTAALNEAKKQLNSALKHAAKINTGSFDFNQKFSFNKEYVKGLEAIKGNDLNAIKEITFKLNSFTYKFSNITKTKNSNKN", "aa_hexdigest": "b62d56354c68c028b8339a5625459047", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00158DF07F", "ncbi_nrp_id": "WP_175936866.1", "uniref100_id": "UniRef100_UPI00158DF07F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158DF07F", "RefSeq:WP_175936866.1", "UniRef:UniRef100_UPI00158DF07F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158DF07F", "uniref90_id": "UniRef90_UPI00158DF07F" }, "psc": { "uniref90_id": "UniRef90_UPI00158DF07F", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI00158DF07F" }, "pscc": { "uniref50_id": "UniRef50_UPI00158DF07F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158DF07F" ], "product": "variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_1155", "locus": "GCA013368015_01155" }, { "type": "cds", "sequence": "contig_1", "start": 670260, "stop": 670379, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGA", "aa": "MYGLVFYLDKEILKKEYSDSNHKEAYEKVRDILRHYGFH", "aa_hexdigest": "3e2077c335083dcd249c2a105b696001", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.4392, "isoelectric_point": 6.817859840393066 }, "id": "LMJBGEANGM_1156", "locus": "GCA013368015_01156" }, { "type": "cds", "sequence": "contig_1", "start": 670752, "stop": 671429, "strand": "-", "frame": 1, "gene": null, "product": "Leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0", "UniRef:UniRef90_D1J8R0" ], "nt": "TTGCTAGATTTATCAAAAACCAATATAACTTCAATACCCGCAGGTGCATTTTCTTATTTAGTTATGCAAAATTTAGCAGACGATGGTGCCGGCTTACCAACCGAATTAAAAACATTAGTTACAACTAATTCTTCAAATAACAACAAAAAAATAAATATTAGAAAAATTATTTTGCCAAAAACCTTAAAATCAATTGGCGCAGGTGCTTTTTATGGATTAAGTAACCTAGAAGAAGTAGATTTTTCTCAATGCAACGACTTATCTCAAATAGATGCTGAAGCTTTTTCAAATACCAATATTAAGAAATTACAATTGCCTGCATCTTTAAATAAATTAGGACAAAAAGCTTTTGCAAACAGTGGCATTACATCTGTAAATTTAGAAGATTTACCTTATTTAAAAACTCTTACACAAGGTGTTTTTGCTTCAAATAAGCTTACAAACATAAACTTAAAAAACATTGAATTAGTTGAAAGTGGAGCCTTGGCAGATAATCAAATAAAAACAATAGAAATTCCTTCAACAATGAAATCATTTAGTCCTGAATCATTTGACTTTATTGGTGCAGGGGAATCAGCTGCTAAAACTCTTATTTCTAAAATTGAAAACAATGAAATTAAATCATTTTTAGAAGATGCTTTGAAGAAAAACCCAAATCATCTATATAAATTAAGCTAA", "aa": "MLDLSKTNITSIPAGAFSYLVMQNLADDGAGLPTELKTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTNINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFIGAGESAAKTLISKIENNEIKSFLEDALKKNPNHLYKLS", "aa_hexdigest": "369829e3a7e32c5111ac5b8d1555fba9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 670752, "stop": 671615, "observations": { "mutation": [ 671447 ], "stop": [ 671447 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8R0", "query_cov": 1.0, "subject_cov": 0.7839721254355401, "identity": 0.973, "score": 390.0, "evalue": 1.38e-135, "gene_start": 1, "gene_end": 225, "reference_start": 63, "reference_end": 287, "reference_sequence": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNHVQGPLKDPKNSTNHNQTWFAESGLLDLSKTNITSIPAGAFSYLVMQNLADEGAGLPTELSTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTNINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFIGAGESAAKTLISKIENHEIESFLKDALKKNPNHLYKLS" }, "paralog": false, "identity": 0.9721254355400697, "score": 515.0, "evalue": 4.4e-187, "description": "Internal stop codon at 671447. Nonsense mutation around 671447.", "psc": { "uniref90_id": "UniRef90_D1J8R0", "product": "Leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8R0", "UniRef:UniRef50_D1J8R0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0" ], "product": "Leucine-rich repeat domain-containing protein" } }, "id": "LMJBGEANGM_1157", "locus": "GCA013368015_01157" }, { "type": "cds", "sequence": "contig_1", "start": 671445, "stop": 671615, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAACAAAAAATTTTTATTTTTAGGCTTTGGTTTAGTTGCTCCATTTATTGCTGGATTAACAGCAATATCTTGTGGAGGAGAAAAGTTCAATGCTTTTGCTCTAAACTATGTACAAGGACCGCTAAAAGACCCTAAGAACTCAACCAATCACAATCAAACTTGA", "aa": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNYVQGPLKDPKNSTNHNQT", "aa_hexdigest": "7feac059743a39b3b7b2d704eccb07de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6088.023000000003, "isoelectric_point": 9.809396553039551 }, "id": "LMJBGEANGM_1158", "locus": "GCA013368015_01158" }, { "type": "cds", "sequence": "contig_1", "start": 671709, "stop": 673679, "strand": "+", "frame": 3, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_175936868.1", "SO:0001217", "UniParc:UPI00158E5D10", "UniRef:UniRef100_UPI00158E5D10", "UniRef:UniRef50_B3PLX9", "UniRef:UniRef90_D1J8R1" ], "nt": "ATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCAGCCGGCGACCAACCTCAGGCAATAAATGAGTTGGTTCACGGTGTTTTAGAAGGAAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACTATGGCCAATGTCATTAATCAGTTAAATAGGCCTACATTAGTTATAAGTCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCTGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCATACATTCCTAAGTCTGATTTATATATTGAAAAAACAAGTAGAAACAATAAAGATTTAGAAGCAATGAGAATGTCTGCTCTTAACGCCTTAAGTATTCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCTGAATTTAACCCAAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTACGCGATCTTTTAACTAAATTGGTTAATTTAGGTTATGAAAAACACAATGGTGATTTATCTTCTGGACAATTTTTTAATAAAGGGGAAGTTATTGAAATTTGTCCCGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTTCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAACGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGTGAATTGAAAGAAAGAGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCCCAAAGGATTCATGATAAAACTTTAAACGATATTGATTCATTAAAGGAATTTGGCTATGTTAAAGGCATTGAAAATTACGCTCGATATGTTGATAAACGTGAACCCGGACAAAAACCATACACAATATTCGATTATTTACCAAAGGATGCAGTAATTTTTGTTGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTACGAAGGAGATCGTTCTAGAAAAGAAACATTGATTAAATATGGTTTTAGATTACCTTCAGCCTTGGATAATAGACCTTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACTCCTGGCGACTATGAAATAAATAAGGCAAATGGTGAACTAGTTACTCAATACATTAGACCGACAGGCCTTTTAGATCCCATTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGACTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTGACTACAACCAAAAAGAATGCCGAAGAGCTTTCTTTATACCTTTCTGAATTTAAAATTAAAAGTGCATATATTCATGATGGATTTAAAACCTTTGAGAGAAATGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTCAGTTTGATTTGTGTATTAAATGCAGATTCTATGGGGCTAATGCGTGATGCTAGAAGTTTGATTCAAATAGTTGGTCGAGCCTCTAGAAATGATCATGGAAAAGTAATATTTTATGCCGATAATATTACAACCAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGAACATTACAAATTAAATATAATAAAGAACACAACATCACTCCTAAAAGTATTATTAAACCAATACCCGCGCCTATTCAAGGTGGTATTTTATTAGAAGATAATAAAGCAATAAAAGAAGCCAATAGGACTATTCATAATAAAAAATACATTGATAATATGATTAAACATATGAAAGAACTAGCGAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGAGATTATTTGATTGAATTAGGAGTCGAATTTGATGATTAA", "aa": "MEKFKLVSNYKPAGDQPQAINELVHGVLEGKEHQVLLGVTGSGKTFTMANVINQLNRPTLVISHNKTLASQLYSELRELFPENRVEYFVSYFDFYKPEAYIPKSDLYIEKTSRNNKDLEAMRMSALNALSIRRDTIVVASVAAIYAEFNPKEYIQSFMPIESHMKIKLRDLLTKLVNLGYEKHNGDLSSGQFFNKGEVIEICPGYTSENNIRIEMFDDYIERISLINPLTKSIVEVRKSLTIFPATTYNVEKGYIENICNSIESELKERVAYFKSQNQLLEAQRIHDKTLNDIDSLKEFGYVKGIENYARYVDKREPGQKPYTIFDYLPKDAVIFVDESHLMIPQIIGMYEGDRSRKETLIKYGFRLPSALDNRPLKFNEYEEYKFPRIYMSATPGDYEINKANGELVTQYIRPTGLLDPIIEIHSKENQIQDIHDLLKLQIEKKERTLILTTTKKNAEELSLYLSEFKIKSAYIHDGFKTFERNEILKGLRMGKYDVVIGINLLREGIDLPEVSLICVLNADSMGLMRDARSLIQIVGRASRNDHGKVIFYADNITTSMQKAIVDNEHKRTLQIKYNKEHNITPKSIIKPIPAPIQGGILLEDNKAIKEANRTIHNKKYIDNMIKHMKELARAKKYEEAIEIRDYLIELGVEFDD", "aa_hexdigest": "ef98cf2c95a266663a1fde1cfdb380a9", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158E5D10", "ncbi_nrp_id": "WP_175936868.1", "uniref100_id": "UniRef100_UPI00158E5D10", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E5D10", "RefSeq:WP_175936868.1", "UniRef:UniRef100_UPI00158E5D10" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E5D10", "uniref90_id": "UniRef90_D1J8R1" }, "psc": { "uniref90_id": "UniRef90_D1J8R1", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_B3PLX9", "cog_id": "COG0556", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLX9" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "LMJBGEANGM_1159", "locus": "GCA013368015_01159" }, { "type": "cds", "sequence": "contig_1", "start": 673767, "stop": 673937, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D75D06", "UniRef:UniRef50_UPI00093FD78B", "UniRef:UniRef90_UPI0002D75D06", "UniRef:UniRef90_UPI00093FD78B" ], "nt": "TTGAAAACTGTTTCTAATAAAAGAAAGATTTGGAATAATGGATATATTGCTAATAAAGTATTGCCAAATGTCAAATATACTGGTGACACTCTTAAAGTTTTTGAATCTAGATATAAAGCAGTTAGAAAATCATTTGCCGAATTGGAAGAGGTACTAAAGAATAATCAATAA", "aa": "MKTVSNKRKIWNNGYIANKVLPNVKYTGDTLKVFESRYKAVRKSFAELEEVLKNNQ", "aa_hexdigest": "3cdfd83f453bf736c783e28c2bccd63e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0002D75D06", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9640000000000001, "score": 105.0, "evalue": 4.28e-29, "valid": true, "uniref50_id": "UniRef50_UPI0002D75D06" }, "pscc": { "uniref50_id": "UniRef50_UPI0002D75D06", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002D75D06" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 673767, "stop": 674066, "observations": { "mutation": [ 673935 ], "stop": [ 673935 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00093FD78B", "query_cov": 1.0, "subject_cov": 0.5656565656565656, "identity": 0.982, "score": 107.0, "evalue": 2.42e-29, "gene_start": 1, "gene_end": 56, "reference_start": 1, "reference_end": 56, "reference_sequence": "MKTVSNKRKIWNNGYIANKVLPNVKYTGDTLKVFESRYKAVRKSFAELEEELKNNQQKSEVFLENILNYLPESEFDLVVTSPTYPFATYFVKYNRLTYY" }, "paralog": false, "identity": 0.9696969696969697, "score": 196.0, "evalue": 1.28e-66, "description": "Internal stop codon at 673935. Nonsense mutation around 673935.", "psc": { "uniref90_id": "UniRef90_UPI00093FD78B", "uniref50_id": "UniRef50_UPI00093FD78B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00093FD78B", "UniRef:UniRef50_UPI00093FD78B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00093FD78B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093FD78B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6562.513700000001, "isoelectric_point": 9.99590358734131 }, "id": "LMJBGEANGM_1160", "locus": "GCA013368015_01160" }, { "type": "cds", "sequence": "contig_1", "start": 674210, "stop": 674392, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_041359654.1", "SO:0001217", "UniParc:UPI000306A4EB", "UniRef:UniRef100_UPI000306A4EB", "UniRef:UniRef50_UPI00093F79AF", "UniRef:UniRef90_UPI00093F79AF" ], "nt": "ATGGCTGTTGTGGATACATACAGACAAAAGGAAAAAGTCTCTTTTGTTGAATGGCTATATGATATATTTCAAAGCAATGGTTCGGTTTTGTTTTCTGATGAAATCAGAGACATTCAAACTCAAACAATGACCCAAAAAAGAATTCAAAAAGAGCATAAATTGGTTTTTAAGAAGGCGGTTTAA", "aa": "MAVVDTYRQKEKVSFVEWLYDIFQSNGSVLFSDEIRDIQTQTMTQKRIQKEHKLVFKKAV", "aa_hexdigest": "9455a520272adc85ed82f2e5bbffbbb4", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000306A4EB", "ncbi_nrp_id": "WP_041359654.1", "uniref100_id": "UniRef100_UPI000306A4EB", "db_xrefs": [ "SO:0001217", "UniParc:UPI000306A4EB", "RefSeq:WP_041359654.1", "UniRef:UniRef100_UPI000306A4EB" ] }, "ips": { "uniref100_id": "UniRef100_UPI000306A4EB", "uniref90_id": "UniRef90_UPI00093F79AF" }, "psc": { "uniref90_id": "UniRef90_UPI00093F79AF", "uniref50_id": "UniRef50_UPI00093F79AF" }, "pscc": { "uniref50_id": "UniRef50_UPI00093F79AF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093F79AF" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7182.1968, "isoelectric_point": 9.099341773986819 }, "id": "LMJBGEANGM_1161", "locus": "GCA013368015_01161" }, { "type": "cds", "sequence": "contig_1", "start": 674633, "stop": 676060, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936871.1", "SO:0001217", "UniParc:UPI0015886DDF", "UniRef:UniRef100_UPI0015886DDF", "UniRef:UniRef50_D1J8R2", "UniRef:UniRef90_D1J8R2" ], "nt": "ATGGATGAAAGAGTGATTGATAAATACAAGATACATTTTATTAATGATAAAAGATACTATGAGTTTGATATGACTAATTTATTACCATCTCTTGATGAAACTATCCCTTATTATTTTAAGTATGATGATATTGAGATATATTCTAATTCTTGGAATAGAATGACGCTTAGTATATTATCTGCTTTGGATAATAAAAATCATAAATCGAATGATGAATTATTGATGATACATTATTTTTGGACCAAAACAGATATATTCTCTTCTGAGAAAAGAACTAATTATACACCATTTAGAGATTTGTACTTGAATACAAATCATACATCTGCACATGCAATGATGAATATTCAAGGATTACTTAAAGCCTATAATATTCCATTAGAAAAATGTTACTTTTTAATAAGACGACATATTTCTGCTGAGCCAGAAGAAGTAAAAAAGGGCATAAGAGCTGATACTATTTTTGCATTTTCACGTTCTTTACAATTAAAAGGATATTCGAGTGATAGGATAGGAGTAATAGTTTCTAATTTTCGAACAATTAATGAGATATTATCTAAGGTAAGTCCAGGTTATAATGATTTTTTCTTATTTGATGATTACTATTATTTTACGAATTATAAAGCTAAACTAGTTGAGTGGTTAGAAAAAAGACATTATTCCGAACAAGATAAAACTTATAGGGCAGTTAAAAGATGTTTAGATTTATTAGGTGATTTTTATAAAAACAAAAATTTTTATAATGATCTTTCGAATACAATTATTACCAATGAGACAATCAAGTTTTTGGGTGATGAAATTGAAAACTTATTTTTATCATTAAACACTGATGTAATTGTATCTAATAAGTTATATGCAAGAATGAGAATGGTGCATTATGAACTACTTAAAAGCATTAACCAATTGAATAATCCCAAAAGCATTTATAAGTTAGCAAGTATCTATTTTGGAAAAAAATATTATTTTAAAGAACCTTTTATTAGCAAAGATAAAAGTGCAAACTTATCAAATGATGAAATAATTTATTCATACGCTTATACAATGGATGAGATTTCAATCTTAAAACTAAATCAATATGCTGATAAAATGCAGCTAAAAAAACTTGATAATTATCTTTTGCTTTTTGAAGATGCTTCAGATGAGTATATTCAAATTGATGAATCAAAACTAATAAAAAAAGATAAAATCGACATAGCTCCAGCTGTTTTAGATAAAATTGAAAAAGAATTATTATATTATCTTGATAGCTTTGGCAGTATAGATAGTGAAACATATGCTGGCTACAATTCAATGCCTTCATTAAATGTAAGTTGGAATAAATACTTATTATTAGGATTGTGTAGAACATATTTTAATGAATTAATTTCTATAAAATATAATCGCAAACAATATAAAAAAACTACCTTTAAATTAGAGTTAAAACAAAAATAA", "aa": "MDERVIDKYKIHFINDKRYYEFDMTNLLPSLDETIPYYFKYDDIEIYSNSWNRMTLSILSALDNKNHKSNDELLMIHYFWTKTDIFSSEKRTNYTPFRDLYLNTNHTSAHAMMNIQGLLKAYNIPLEKCYFLIRRHISAEPEEVKKGIRADTIFAFSRSLQLKGYSSDRIGVIVSNFRTINEILSKVSPGYNDFFLFDDYYYFTNYKAKLVEWLEKRHYSEQDKTYRAVKRCLDLLGDFYKNKNFYNDLSNTIITNETIKFLGDEIENLFLSLNTDVIVSNKLYARMRMVHYELLKSINQLNNPKSIYKLASIYFGKKYYFKEPFISKDKSANLSNDEIIYSYAYTMDEISILKLNQYADKMQLKKLDNYLLLFEDASDEYIQIDESKLIKKDKIDIAPAVLDKIEKELLYYLDSFGSIDSETYAGYNSMPSLNVSWNKYLLLGLCRTYFNELISIKYNRKQYKKTTFKLELKQK", "aa_hexdigest": "32bd99d25fa872341cb3aa56d02d9468", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0015886DDF", "ncbi_nrp_id": "WP_175936871.1", "uniref100_id": "UniRef100_UPI0015886DDF", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015886DDF", "RefSeq:WP_175936871.1", "UniRef:UniRef100_UPI0015886DDF" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015886DDF", "uniref90_id": "UniRef90_D1J8R2" }, "psc": { "uniref90_id": "UniRef90_D1J8R2", "uniref50_id": "UniRef50_D1J8R2" }, "pscc": { "uniref50_id": "UniRef50_D1J8R2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R2" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 56621.06579999993, "isoelectric_point": 8.261897468566897 }, "id": "LMJBGEANGM_1162", "locus": "GCA013368015_01162" }, { "type": "cds", "sequence": "contig_1", "start": 676173, "stop": 676400, "strand": "+", "frame": 3, "gene": null, "product": "Histidine kinase", "db_xrefs": [ "RefSeq:WP_175936872.1", "SO:0001217", "UniParc:UPI00158EAF17", "UniRef:UniRef100_UPI00158EAF17", "UniRef:UniRef50_A0A9X3K6U9", "UniRef:UniRef90_UPI0027337EB7" ], "nt": "ATGAAAAATAAAAATGATAATTTTAAACGTATATCTGAAAACAGAGTATCAAAGATTTTGGCTTTACTCTCTCAGTTAACGAATCTTAGTAATTCATCTTTTTATGAATATACTGATGAGGATATAGAGAAAATGTTTAATGTCATTTATGAAGAAGCAAAAAAATCAAAGGAAATATTGCTAAAGTCAAATGATAAAAAAAGAAAGAATAAAAGGTTTGAGTTATAG", "aa": "MKNKNDNFKRISENRVSKILALLSQLTNLSNSSFYEYTDEDIEKMFNVIYEEAKKSKEILLKSNDKKRKNKRFEL", "aa_hexdigest": "cf873b56695248ee7d14b530dea1db19", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00158EAF17", "ncbi_nrp_id": "WP_175936872.1", "uniref100_id": "UniRef100_UPI00158EAF17", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EAF17", "RefSeq:WP_175936872.1", "UniRef:UniRef100_UPI00158EAF17" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EAF17", "uniref90_id": "UniRef90_UPI0027337EB7" }, "psc": { "uniref90_id": "UniRef90_UPI0027337EB7", "uniref50_id": "UniRef50_A0A9X3K6U9" }, "pscc": { "uniref50_id": "UniRef50_A0A9X3K6U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9X3K6U9" ], "product": "Histidine kinase" }, "genes": [], "id": "LMJBGEANGM_1163", "locus": "GCA013368015_01163" }, { "type": "cds", "sequence": "contig_1", "start": 676413, "stop": 678368, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936873.1", "SO:0001217", "UniParc:UPI00158B7942", "UniRef:UniRef100_UPI00158B7942", "UniRef:UniRef50_D1J8R3", "UniRef:UniRef90_D1J8R3" ], "nt": "ATGTCAGACAATAAGTGGAGATTCCCTGCAAGTAATCATGGTGAAAAAAAAGGTATTAGTAGTGGCGATACAGAAACATTTAAAAAGTCACCTTTCCAAGCGTTCGCGAGAGAAATCTTGCAAAACTCTATTGATGCTAGGTCGAGTGATGAAGAACCAACTAGAGTTGAATTTTATCTTTTCAAAATAAAAACAGATGATATTCCAGGCAAACAAGAATTATATGATGCTATAAGACGTTGTAAAGAATTTTGGTCTTATAAAAAGGATTATGTTGATGAGTATGATTCTATGATGAAGTTACTTGAATCAGAAAACATATGGTGTCTTCGAGTAAGCGATTTCAATACTACTGGCTTAATAGGCGTAGAAACCACTAAGCAAGAGAATAATAAGTTTTTAGCATTAGCAAAAGGAACTGGTGTTTCAGAAAAAAGTGGTGTAATGGCTGGCGGTTCAAAAGGTGTAGGCAAAAATGCAGCGTTTTTAATGTCTTCTTTGAGAACAGTTGTTTATTCTACTAGAAGTAATCAAGATATTGATGGTAATCCTGGAGTCTTTAAAGGCTCCATAGGTGTTGCTGAGCTTGTGTCTGGATATATTGATGATATTGCTATTGAGAATAGAGATTATACGCAAGGCACAGGATATTTTAGCTGCGATGATTTAAACGGCGCGATAGATAAAGTATATAATTTGGATCGTGATTTTACTTCAAGAAATAGTGAATATGGAACTGATATTTATATTATAGGGTTTAATAACGAAGATAATTGGCAAAAAGAAGTTGTTAATAGTTTGTTGGATTCATTTATGGCAACCATAGTCAGAGGTGAATTAGAAGTTAACATTAATGGAAAAGAAATAAATAGGGATACTATAAAGGAAATCGTATATGACAATTCTATTATCAAAAAAAGTAATCGTAGTAATGTGATTTCCCAATATCGATTATTAACTAATGAAGATGGAAAAGTATTGACATATGATATCGATACTGAATATGGAAATTGTGAATTATATATTTTACCATATGATAAATCTGAGGAAGATTTGGCTACTAATATGTGTGCGATGATTAGGTCCCCATTAATGAAGATTAAAGATGAAAAATTGGGACCCTGTTTTAGAGTTTCAGCTATGTGTATTATCAACGAAGGTACATTGGGTATTAAACTTAGAGATATAGAAAATGCACAACATACAAATTGGGAAGTAAACAGAATAAAAGATGTTTATGAAAGAAAAGAAATACAAAGTGTTATTGATAGTATAAAACAACAAATAAATGATAGTGTTATAGATTGCTTAAAATTGGGTGATAATGAACCCTTAGATCCTAATGGTGCAGGAGATTATTTACCTGATGTTGATAATGGTGGTGATTCAAGTTCTGGTGCTAATGGAAAGCAAAAACCAATTGAAAATGTATCTATCTCAAAACCAAAAACCAATAAGGTAGTAGAAAAAAATACTAACCAAGAAAATGACAACGGTGATGGTTTAGAACCTGATATTGGAGAAGTTGATGATAATATTGATGGTGATGTTCAACATCCTATTGGTACAAATGATAAATCAAATGAAGGAAGACACCCAGGAAGTGAATCTAGTGGTGAGCAAGAAGGCGAAAATATTGTATTTAAACGTGCTAAATTATCTGGTGTTAAATATAAAGTAATCTCTACTAATAAAAACGAAGGCAGATTAAGAATTATTTTTACGGCTCCAATTGATTTTGAAACTTGTTATTTGAATATTGCTTTATTAGATGATGCTAATAATTCTGTTGCAGTAGATATTGAAGAATTGAATTGTAATGGAGTTAATGTTTATGGTGAAGATAAAAAAGAATATGGCCCATTCAAAATAAGCCGTAATGATAAGATTATTTTAAATGTAAAGACTAATTTAAACGGCTATTTCGCTAGTGAGGTGAAAGTAATATGCAAGTAA", "aa": "MSDNKWRFPASNHGEKKGISSGDTETFKKSPFQAFAREILQNSIDARSSDEEPTRVEFYLFKIKTDDIPGKQELYDAIRRCKEFWSYKKDYVDEYDSMMKLLESENIWCLRVSDFNTTGLIGVETTKQENNKFLALAKGTGVSEKSGVMAGGSKGVGKNAAFLMSSLRTVVYSTRSNQDIDGNPGVFKGSIGVAELVSGYIDDIAIENRDYTQGTGYFSCDDLNGAIDKVYNLDRDFTSRNSEYGTDIYIIGFNNEDNWQKEVVNSLLDSFMATIVRGELEVNINGKEINRDTIKEIVYDNSIIKKSNRSNVISQYRLLTNEDGKVLTYDIDTEYGNCELYILPYDKSEEDLATNMCAMIRSPLMKIKDEKLGPCFRVSAMCIINEGTLGIKLRDIENAQHTNWEVNRIKDVYERKEIQSVIDSIKQQINDSVIDCLKLGDNEPLDPNGAGDYLPDVDNGGDSSSGANGKQKPIENVSISKPKTNKVVEKNTNQENDNGDGLEPDIGEVDDNIDGDVQHPIGTNDKSNEGRHPGSESSGEQEGENIVFKRAKLSGVKYKVISTNKNEGRLRIIFTAPIDFETCYLNIALLDDANNSVAVDIEELNCNGVNVYGEDKKEYGPFKISRNDKIILNVKTNLNGYFASEVKVICK", "aa_hexdigest": "1cb705598c020b1325a68bf8d1c69893", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158B7942", "ncbi_nrp_id": "WP_175936873.1", "uniref100_id": "UniRef100_UPI00158B7942", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B7942", "RefSeq:WP_175936873.1", "UniRef:UniRef100_UPI00158B7942" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B7942", "uniref90_id": "UniRef90_D1J8R3" }, "psc": { "uniref90_id": "UniRef90_D1J8R3", "uniref50_id": "UniRef50_D1J8R3" }, "pscc": { "uniref50_id": "UniRef50_D1J8R3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 72802.2855000002, "isoelectric_point": 4.787514305114747 }, "id": "LMJBGEANGM_1164", "locus": "GCA013368015_01164" }, { "type": "cds", "sequence": "contig_1", "start": 678359, "stop": 679273, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175936874.1", "SO:0001217", "UniParc:UPI00158CD30F", "UniRef:UniRef100_UPI00158CD30F", "UniRef:UniRef50_D1J8R4", "UniRef:UniRef90_D1J8R4" ], "nt": "ATGCAAGTAAAGAATAAAATATTTCCTTATCCTGTTTTAAATAATAATAAACTATATTCAAATTTTGGAGATTATTCATTTGAAATCATGTATGAATCTTCTGAGGATGAATATGCATATATTTTAAAAGATATTAAATTTTCAACTGAAAGTAAATTTATAAATGATTTGTACGATAAAAAATTGATTAAAGTTTTTTTAGTTGTAGAATGCTCTGATACTGTTTTTAGAAAAAAAATAGAAGTGGATAAGATATCTTCAACGATACGTCTTTATAAAAATGATTTTACAGAAAAGGTAGATATCTCGATGTTTGCAGTTGCAACAAAGAATTTTACAGTTTTTTCTAGTGAAGTTGACGAAGAGTATCAAGATATTGAATTTGAAATTGAAAAGTACGATATCTTATGTGCAAATGATGGATTTAATGTTAGATTTAAGCATGATGAATCAGAGAATAATTTAGTTAAATCAATTTTCTCAATTATTCCAAGCGATAGTATTAACGATGGTACATTTGAAGTTAATTGTGAGTTAGGAAAAAAGATTACTATTTCTATGTCTGACACTGATTTTAAAAATATGAATATAGTTAATACTGTTCCAACATATAAAGAAGTAGTATTTAATATGCTATTAGTTCCCGCATTAATTGAAGGTTTGACTTTGTGTTCGAAAACTGTTCAAGAAGGAACAGATGATTTAGATGATATTGGAAATAAATACGTCTGGTTTAGAAGTATATTAAAGTCATATAAACGTTTACATGGAAAAGATATTACAATTGATGAATTTAAAAGTTTATCGCCTGTTCTTTTAGCCCAACAATTACTAGGGAAACCACTAGGTGCAGCTTTACATAAGCTTGTTGTTGAAACAAATAAAATAGATGAAGGAGGAAATGATAATGAGTAA", "aa": "MQVKNKIFPYPVLNNNKLYSNFGDYSFEIMYESSEDEYAYILKDIKFSTESKFINDLYDKKLIKVFLVVECSDTVFRKKIEVDKISSTIRLYKNDFTEKVDISMFAVATKNFTVFSSEVDEEYQDIEFEIEKYDILCANDGFNVRFKHDESENNLVKSIFSIIPSDSINDGTFEVNCELGKKITISMSDTDFKNMNIVNTVPTYKEVVFNMLLVPALIEGLTLCSKTVQEGTDDLDDIGNKYVWFRSILKSYKRLHGKDITIDEFKSLSPVLLAQQLLGKPLGAALHKLVVETNKIDEGGNDNE", "aa_hexdigest": "22ddbbdf5a8efb62503738d2e51b5c02", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00158CD30F", "ncbi_nrp_id": "WP_175936874.1", "uniref100_id": "UniRef100_UPI00158CD30F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158CD30F", "RefSeq:WP_175936874.1", "UniRef:UniRef100_UPI00158CD30F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158CD30F", "uniref90_id": "UniRef90_D1J8R4" }, "psc": { "uniref90_id": "UniRef90_D1J8R4", "uniref50_id": "UniRef50_D1J8R4" }, "pscc": { "uniref50_id": "UniRef50_D1J8R4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 34909.315700000014, "isoelectric_point": 4.796324348449708 }, "id": "LMJBGEANGM_1165", "locus": "GCA013368015_01165" }, { "type": "cds", "sequence": "contig_1", "start": 679266, "stop": 680030, "strand": "+", "frame": 3, "gene": null, "product": "DUF6339 domain-containing protein", "db_xrefs": [ "RefSeq:WP_175936875.1", "SO:0001217", "UniParc:UPI0015893B44", "UniRef:UniRef100_UPI0015893B44", "UniRef:UniRef50_D1J8R5", "UniRef:UniRef90_D1J8R5" ], "nt": "ATGAGTAAAATCAACATTAAAATGCTTTCAGAAGATGCTTTAGTATATTTAAAAGAAAACGCTGAAAGTATTGCAGAGAGAATTGCAGAAAATGAAGATAATTCTTGGATTAAAGATACCATTCCTCAATCTATACGACTATTTGTTGAAAAGAAATTACAAATTGAAGATTTTGATTTATTAGACAATCCTGAATCTGCAGATAAAAATATTGATTTTCAAAATTCTATTAAAATATACGAATCGTTAAATGAACTTCCTAGATATATTTTATGCGACCAAAGATTCTGGCTTTGGCTTCACTTGGATAAGTTTTACAAAACAGTTAGAAATATGATGAAGATCAGAGGAAAGTCTACATTTACAGACCATTGGCTTCAATCTGGAGGAACTAGAAGAGGATTGATGTTTGGTGTACTATCAAGATGCTATTTCAGAGTTGCTTTAACAGTTGATGAAGGTAAAGCTAATAAATATGAATTAACAAAATGGGTAATTGAAAATCCAGAGAGATATAGAAACCTTACATGGCGTTCTTTTTCATCAGAAGAACATTTAGTTAGAGGCGTTTTGAAGGGTGAAAAGAAGGCGATTGATGAAAACCCAAAAAAGGAAGCAAATAATCTTTATCCAGAAATTGCTAAATATGTATCTATTATTGGAAGCGTTAGGTTGTTAGATTACATATCTGAAGAAGATATAGAAAAGATGATTTATGATAAGATGATAGAGTATATTAATAGTAGTGGCGGAGATGAGAAGTAG", "aa": "MSKINIKMLSEDALVYLKENAESIAERIAENEDNSWIKDTIPQSIRLFVEKKLQIEDFDLLDNPESADKNIDFQNSIKIYESLNELPRYILCDQRFWLWLHLDKFYKTVRNMMKIRGKSTFTDHWLQSGGTRRGLMFGVLSRCYFRVALTVDEGKANKYELTKWVIENPERYRNLTWRSFSSEEHLVRGVLKGEKKAIDENPKKEANNLYPEIAKYVSIIGSVRLLDYISEEDIEKMIYDKMIEYINSSGGDEK", "aa_hexdigest": "cfecaad7ed7c8e2bb2314561e45abf87", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0015893B44", "ncbi_nrp_id": "WP_175936875.1", "uniref100_id": "UniRef100_UPI0015893B44", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015893B44", "RefSeq:WP_175936875.1", "UniRef:UniRef100_UPI0015893B44" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015893B44", "product": "DUF6339 family protein", "uniref90_id": "UniRef90_D1J8R5" }, "psc": { "uniref90_id": "UniRef90_D1J8R5", "product": "Family of unknown function (DUF6339)", "uniref50_id": "UniRef50_D1J8R5" }, "pscc": { "uniref50_id": "UniRef50_D1J8R5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R5" ] }, "genes": [], "id": "LMJBGEANGM_1166", "locus": "GCA013368015_01166" }, { "type": "cds", "sequence": "contig_1", "start": 680073, "stop": 681359, "strand": "+", "frame": 3, "gene": "dam", "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "COG:COG0338", "COG:L", "EC:2.1.1.72", "KEGG:K06223", "SO:0001217", "UniRef:UniRef50_D1J8R6", "UniRef:UniRef90_D1J8R6" ], "nt": "TTGAAAAGAATAAATGAGTTCATTAATGATTCTGTTGAACCTGGTTCAAGTTTTACATATTTAGAACCCTTTCTTGGTGGAGGGGCAGTATTCTTTGATAAAAGACCAAAGAAAGCTATCATTAATGATCTAAACGAAGATTTAATTAACGCCTATAGAGTTATTCAGTCTGATCAATATGAAGAGTTGATTAGACTTTTGGATGTGCACGCAGAAAACTATAGAAAAGAACCGGACGACTATTACTATGAGGTCAGAGCATGGGATAGAAATCCAACTAGATGGCCAAACGGTTATACAAATGTTCAAAGAGCTGCTCGTATGATTTTTTTGAATCGAACATGCTATAATGGATTATATAGGGTAAATAATAGGGGCGAATTTAATACACCTATTGGTAGATATCATAATCCTTTAATATGTGACAGAGAAAATTTAAAAGAAATTCATGATTATTTATCTTCAAAAGAAAATGAAATAACAATAATGAATGGCTCTTATGAAGAGTGTGTAAGATTAGCTAAAGATGGTGATGTTATTTATTTAGATCCTCCATATGATTATGAAGATGATGATGGCTTCACTAAATATCAAATGGCCGGTTTTTCATTTGAAAATTTCCGTGAATTAAAGAAGGAATGTGATAAAGCACTGAGTAAAGGTGCGTTTGTAATTATCAGTAATAATGCAACTCAAAAAGTTACAGAACTATTTGAGCAAGACCCTAAATATAAGATTTTTTATGATGTAAATAAGTTTTCAACATTAAGAATTATTAATTGTAATGGCGCTGAAAGAAGAACTGGAAGAGAAGCTATATTCTGGGGAATGGATAATAATATTCCTTTTCCACAGGCAAATGATATGACTAAAATTATAAAACTTGTTATGGCTGATGAATCTGTGATTAATGATAAGGCAAAAGCCATGGAATTAATCCATGTTAGCACGACTAGACAAGTAGCATATTATTTCTCTGCACTTCAATTTTTAAAGTATTTAAAGCATGATAAGACATTTACTGATAGAGCAAATAGTTTGATGAAAAATAAAAGCAAAATCATTATCGACATTTATGATCAATTATTAAATGATGATATGTTCGGTAATGCTTATAAACAATTTAAAGATTCAAAAAAGGTAAATGTTGAAGTTATTAAAAATGAAATAAGAAAAAATAAGACTAATTTGGCGGACTCAACTGTAAATAGAAGAGCATCAACTATTAAGGCATGGGTTGAATGGATGTATACAATTGATGGTCGTATGCGGACGATTAAATCTTAA", "aa": "MKRINEFINDSVEPGSSFTYLEPFLGGGAVFFDKRPKKAIINDLNEDLINAYRVIQSDQYEELIRLLDVHAENYRKEPDDYYYEVRAWDRNPTRWPNGYTNVQRAARMIFLNRTCYNGLYRVNNRGEFNTPIGRYHNPLICDRENLKEIHDYLSSKENEITIMNGSYEECVRLAKDGDVIYLDPPYDYEDDDGFTKYQMAGFSFENFRELKKECDKALSKGAFVIISNNATQKVTELFEQDPKYKIFYDVNKFSTLRIINCNGAERRTGREAIFWGMDNNIPFPQANDMTKIIKLVMADESVINDKAKAMELIHVSTTRQVAYYFSALQFLKYLKHDKTFTDRANSLMKNKSKIIIDIYDQLLNDDMFGNAYKQFKDSKKVNVEVIKNEIRKNKTNLADSTVNRRASTIKAWVEWMYTIDGRMRTIKS", "aa_hexdigest": "e7140e2ff472a9c12f3139cadde218f1", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8R6", "query_cov": 1.0, "subject_cov": 0.9683257918552036, "identity": 0.9840000000000001, "score": 860.0, "evalue": 2.84e-315, "valid": true, "gene": "Dam", "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_D1J8R6", "cog_id": "COG0338", "cog_category": "L", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_D1J8R6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R6" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [ "dam" ], "id": "LMJBGEANGM_1167", "locus": "GCA013368015_01167" }, { "type": "cds", "sequence": "contig_1", "start": 681993, "stop": 682274, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACCAATATAAAATGGAGTTTTTTTATACTATTAGCAATTATTTTTATAATTGCTTTTGCTTTAATGTTAACTAGCTGTGCCTCAACTGATCCTAGAGATGCACAGTTTTATATTAGGGATGATAGCTTTTATTACAATAGTAGCGCAAACATAACAAAAGTTAATTTTCAAGTAAGTTTTGAGAATAATTCAATATATAAAATTGTTAAGAATTCAATAACATTTGATTTGTATGATAATGGAAAATATGCATTTCAAAACTCTTTTAATTGA", "aa": "MKKTNIKWSFFILLAIIFIIAFALMLTSCASTDPRDAQFYIRDDSFYYNSSANITKVNFQVSFENNSIYKIVKNSITFDLYDNGKYAFQNSFN", "aa_hexdigest": "45e483b558d2323e809415acf4199970", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10858.259499999991, "isoelectric_point": 8.732710075378417 }, "id": "LMJBGEANGM_1168", "locus": "GCA013368015_01168" }, { "type": "cds", "sequence": "contig_1", "start": 682761, "stop": 683222, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35" ], "nt": "ATGAACAAATACACTAAAAATCAATTAAATCAAGTTTATAAACATGTGCTCTTAGATATATGCAAAGCTTCTGAATTTAAAGTTGATGAAAAGATAAAAAAAGAAGAATTAATAAATTTAATTTTGTCAAGAAGACCAGATTTTAAAGAAAGCGACAACACAAAAAATCTTTCTAAACCAAAAAACAATGAAATTAAACCATTATTAAAAAGCCCTCCTTCTAAAAAGCCGATGTTTGAAGACATTGTGGGTTTAAACAAAGCAAAAGAAGCGTTTATTGAAAAGATTATTCTTCCTTTCAAACACAAAGAAATATTTGAAAAGTTTAATAAAAAAATAGGTGGTGGCATATTATTATACGGTTTGCCCGGCACTGGAAAAACAATGTTTGCAGAGGCTACTTCTAATGAATTAAATGCTTTATTTATTCCAATAAAGTGTTCAGATATTAAAACTAAGTGA", "aa": "MNKYTKNQLNQVYKHVLLDICKASEFKVDEKIKKEELINLILSRRPDFKESDNTKNLSKPKNNEIKPLLKSPPSKKPMFEDIVGLNKAKEAFIEKIILPFKHKEIFEKFNKKIGGGILLYGLPGTGKTMFAEATSNELNALFIPIKCSDIKTK", "aa_hexdigest": "0c864622f11ddc387a707f10bd81d222", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 35, "aa_cov": 0.22875816993464052, "hmm_cov": 1.0, "evalue": 3.3538715063174027e-09, "score": 32.822383880615234, "start": 117, "stop": 151 } ], "seq_stats": { "molecular_weight": 17509.457999999988, "isoelectric_point": 9.571508216857907 }, "id": "LMJBGEANGM_1169", "locus": "GCA013368015_01169" }, { "type": "cds", "sequence": "contig_1", "start": 683328, "stop": 683450, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAATGCAATTAGTGAAGGCAACGACATAGTTCCACAATTGCTATCCGAAATGCAAGGAATTGATAATTTAAAAAATGAATCAAATATTATTGTTATAGCAGCAACAAATAAACCATGA", "aa": "MDNAISEGNDIVPQLLSEMQGIDNLKNESNIIVIAATNKP", "aa_hexdigest": "7e0735a41115ad7540abd037d63b9e50", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4310.813000000002, "isoelectric_point": 4.0500284194946286 }, "id": "LMJBGEANGM_1170", "locus": "GCA013368015_01170" }, { "type": "cds", "sequence": "contig_1", "start": 683451, "stop": 683801, "strand": "+", "frame": 3, "gene": null, "product": "26S protease regulatory subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000698C617", "UniRef:UniRef90_UPI000698C617" ], "nt": "ATGATAGATTCGGCATTTTTAAGGCCAGGAAGATTTGATGAAAAAATTTATATTCCACTTCCTGACTACGAATCAAGAAAAAAGATGTTTGAAATTCAATTATCTTCGTTGCCAATTGCTGATAATTTCGATTTTGATTATTTAGCAAGCATAACTGAAAATTTTAATGGTGCCGATATTAAAGAAGTTTGTGAAAAAATAAAAATGAGTGCTATTAAAAAGAATATAAACAATAATCAAGAACATAAAATAGAAATGGAAGATGTAAAACAAATAGAAAAAGACATTAAATCTTCTGTTAATCTTCAAGATATTCAAAAATTAAAAGAATTTGAAAAAAATTTTTATTAA", "aa": "MIDSAFLRPGRFDEKIYIPLPDYESRKKMFEIQLSSLPIADNFDFDYLASITENFNGADIKEVCEKIKMSAIKKNINNNQEHKIEMEDVKQIEKDIKSSVNLQDIQKLKEFEKNFY", "aa_hexdigest": "847a42e731ad701bc06aaa609edad917", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 683151, "stop": 683801, "observations": { "mutation": [ 683220, 683448 ], "stop": [ 683220, 683448 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000698C617", "query_cov": 1.0, "subject_cov": 0.5321100917431193, "identity": 0.991, "score": 219.0, "evalue": 5.98e-71, "gene_start": 1, "gene_end": 116, "reference_start": 103, "reference_end": 218, "reference_sequence": "MFAEATSNELNALFIPIKCSDIKTKWYGESEQKIHKIFERARKQEKAIIFFDEFEAIGTKRMDNAISECNDIVPQLLSEMQGIDNLKNESNIIVIAATNKPWMIDSAFLRPGRFDEKIYIPLPDYESRKKMFEIQLSSLPIADNFDFYYLASITENFNGADIKEVCEKIKMSAIKKNINNNQEHKIEMEDVKQIEKDIKSSVNLQDIQKLKEFEKNFY" }, "paralog": false, "identity": 0.9814814814814815, "score": 412.0, "evalue": 1.73e-149, "description": "Internal stop codon at 683220, 683448. Nonsense mutation around 683220, 683448.", "psc": { "uniref90_id": "UniRef90_UPI000698C617", "product": "26S protease regulatory subunit", "uniref50_id": "UniRef50_UPI000698C617", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000698C617", "UniRef:UniRef50_UPI000698C617" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000698C617", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000698C617" ], "product": "26S protease regulatory subunit" } }, "id": "LMJBGEANGM_1171", "locus": "GCA013368015_01171" }, { "type": "cds", "sequence": "contig_1", "start": 683838, "stop": 684230, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R8", "UniRef:UniRef90_D1J8R8" ], "nt": "ATGCAGGTAAAAAATGCCAAAAAATTTGCGTTAGCTACTATAATATTATATTTTATTTGTGTAGTACTTTTAATACCAATGATTATATTAAATTTGATGCAAGATACAATAAAGGAAAATCTTTCTTTGTATTCGGCGATAGCTTTGCTTTCGTTTGCATTAGAATATTTAGGAGCCGCTATAACGTTCATAACGTTTTATATAATTACAATTGTAAATGGTTTTTATTATAAAGAAACGCTTGGATCAATATTAATGTGCGTAGGAATTATTGTGCCTATAGTAGGTATTATTGGAACATTTTTTGTTTATTTTAATCTTAAAAAAATACCGGTTGTTAAATTAACCACGCCAAACACTCAACCAACAGTTGGTAATCCAAATCCATCATGA", "aa": "MQVKNAKKFALATIILYFICVVLLIPMIILNLMQDTIKENLSLYSAIALLSFALEYLGAAITFITFYIITIVNGFYYKETLGSILMCVGIIVPIVGIIGTFFVYFNLKKIPVVKLTTPNTQPTVGNPNPS", "aa_hexdigest": "21e50bf6adf8ad36d3a3c9a91884be3b", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J8R8", "query_cov": 1.0, "subject_cov": 0.8904109589041096, "identity": 0.992, "score": 238.0, "evalue": 6.83e-79, "valid": true, "uniref50_id": "UniRef50_D1J8R8" }, "pscc": { "uniref50_id": "UniRef50_D1J8R8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14400.310599999977, "isoelectric_point": 9.14582347869873 }, "id": "LMJBGEANGM_1172", "locus": "GCA013368015_01172" }, { "type": "cds", "sequence": "contig_1", "start": 684279, "stop": 684374, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAATAAAATAAAAGAATCTCTAGCGATTGTTATATTGCTCCCTATTATTACTGCCTTGAACTTACTTTTTGTATTTACTAGAGGATGA", "aa": "MKRNKIKESLAIVILLPIITALNLLFVFTRG", "aa_hexdigest": "d9f99be9066e1c128f429feb8c88c941", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3526.369400000001, "isoelectric_point": 11.097023582458494 }, "id": "LMJBGEANGM_1173", "locus": "GCA013368015_01173" }, { "type": "cds", "sequence": "contig_1", "start": 684646, "stop": 684945, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTTATTTTGACCCTCCTTATGATACCTTAGAAAATAAACATTCATTTACTTCTTATTCAAATGAAACCTTTGGAAAAAATGAGCAAATTAAACTTGCTAATGTTTTTAAAAAGTTGTCAAAAAAGGGCGTTTATGTAATATTAAGTAATCACAAAACTGATTTTGTTTGCAATTTATATAGTGATTTTAATATTAACATAATTAAAGCAAGAAGAAACATTAACAGAAATGGTAAAAATCGAGGTGATGTCGAAGAGGTAATCATTACTAATTTTAATAAAGAGGTTCTAAAATAG", "aa": "MVYFDPPYDTLENKHSFTSYSNETFGKNEQIKLANVFKKLSKKGVYVILSNHKTDFVCNLYSDFNINIIKARRNINRNGKNRGDVEEVIITNFNKEVLK", "aa_hexdigest": "f96ca24359100dbf7e0bc6a7b1af5d9d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11581.041499999985, "isoelectric_point": 9.380101585388182 }, "id": "LMJBGEANGM_1174", "locus": "GCA013368015_01174" }, { "type": "cds", "sequence": "contig_1", "start": 685005, "stop": 685358, "strand": "-", "frame": 1, "gene": null, "product": "DNA adenine methylase", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "RefSeq:WP_217702915.1", "SO:0001217", "UniParc:UPI001C37D028", "UniRef:UniRef100_UPI001C37D028", "UniRef:UniRef50_UPI0022AA5C41", "UniRef:UniRef90_UPI00215CA48B" ], "nt": "TTGCCTAAGAATTTTTACATCAATGATATTAACAGCGAACTAGTTCTAGCATACAAGTGTTTTACAAAGAAAGAATCATTTGATAAATTAAAATCACTTTTAGACCTTCATACTAGTAAGGATTCCAACGAATACTATTATCAAATAAGAGATTTAGATAGACAACCCGAATATAAAAACTTGTCAGATGAAGAAAAGGCTGCCAGAATTATTTATTTGAATAAAGCATGTTTTAATGGTTTATATCGTGTAAATAAAAATGGTTTTTTAATGTTCCATCAGGAAAGAAAGAAAAAGTCAATTGCTATAATAAAGAAAACTATGATTCTATTTTTAAATATTTTTCTAATGTAG", "aa": "MPKNFYINDINSELVLAYKCFTKKESFDKLKSLLDLHTSKDSNEYYYQIRDLDRQPEYKNLSDEEKAARIIYLNKACFNGLYRVNKNGFLMFHQERKKKSIAIIKKTMILFLNIFLM", "aa_hexdigest": "fce907ca1a9db0f97cb67f58c2e53cd7", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001C37D028", "ncbi_nrp_id": "WP_217702915.1", "uniref100_id": "UniRef100_UPI001C37D028", "db_xrefs": [ "SO:0001217", "UniParc:UPI001C37D028", "RefSeq:WP_217702915.1", "UniRef:UniRef100_UPI001C37D028" ] }, "ips": { "uniref100_id": "UniRef100_UPI001C37D028", "uniref90_id": "UniRef90_UPI00215CA48B" }, "psc": { "uniref90_id": "UniRef90_UPI00215CA48B", "product": "DNA adenine methylase", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_UPI0022AA5C41", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_UPI0022AA5C41", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022AA5C41" ], "product": "Dam family site-specific DNA-(adenine-N6)-methyltransferase" }, "genes": [], "id": "LMJBGEANGM_1175", "locus": "GCA013368015_01175" }, { "type": "cds", "sequence": "contig_1", "start": 685664, "stop": 685846, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGAATTATCATTATTTGCAAACATTGAATATGATGAAAATAAATCATTACATGAACAAGTTGTACAGTTGATAGAAGGAATAAAAAATACTAATTCGCCCAAAGCAAAATTATTAATTAACGAAATTATTGATTTATACAATTCTTATTTTTTAACCTTTGGAAACAACGGATATTGA", "aa": "MKELSLFANIEYDENKSLHEQVVQLIEGIKNTNSPKAKLLINEIIDLYNSYFLTFGNNGY", "aa_hexdigest": "9b37effcdaed9bcea09c284e29b5dada", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6938.774599999999, "isoelectric_point": 4.83730525970459 }, "id": "LMJBGEANGM_1176", "locus": "GCA013368015_01176" }, { "type": "cds", "sequence": "contig_1", "start": 686468, "stop": 686926, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAACGATGAATATTTAGTCAATAAAGAATTATTTAATCCCAAAAACAAAGAAATACCAGTTTATTCAAAATTTAGTCAATATGACTTATTCAAGCTAGCTAACCTCGCCTTAACAAACAAAGAAGTTGAAAAATACAATATTTTAAACAATAAAAACTTTTATTTTAATAATTCAACAAACAACACAGTTTATTATGCAAATTCTACTAATATTGATCAAATAAAATACTTGTTTTCATTTGAGGAACTTTTTGCAAGAGAACTAGTAAAACTTTCGTATTCAGCACATGAAAATTTTCAAATGTTTGACGGCCATTTAACTTTGGATGATTCTTTGAATTTCTTATATTATAAATTAAAAAATGATGATATTTTTCTATCATCAATTGGCGATGACACATTGAAAATGATTGCCAATTATTATAATAAATATGTAATATATTCAGCCAATTGA", "aa": "MQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQIKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSAN", "aa_hexdigest": "5f049ff731051e5c7e1c3bf5367e4449", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18093.072999999997, "isoelectric_point": 5.147134590148924 }, "id": "LMJBGEANGM_1177", "locus": "GCA013368015_01177" }, { "type": "cds", "sequence": "contig_1", "start": 687155, "stop": 687712, "strand": "-", "frame": 2, "gene": null, "product": "MYPU_1760 family metalloprotease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D", "UniRef:UniRef90_UPI001E491E9D" ], "nt": "ATGAGCAAGCAAAAACAAGGTAGAAAAGCATTTCTACCAAAATGGGCAATATTTATTATAATCTTGGCTTTTATATTAACGGCAGGTATTATTGGCGTCGTAATATATTACATTTTTAATTATGTTAATTATTCAAATGGCAATGGTGGGGGTTTATTTGATGGCCAAAAAAATAAAAATTATTCAATGCCAAATGAAGAAAGATATCTACAAAGTTTCAATGCAAAAACATTTGCCAAAAACAATGTTCAATTAAAGAACAAGAATTATCAATATGGAAATTTAAGTATTTTTGAATATCCTTTTGCTTTAACAAAAAATAATGAACCTATTTATTTTTTGGGTGAAGAAGGAAGAAAATTATTGAATTTAGAGTTTTTAAAAAGAGGCTTTTATGGTCCTGAAATTAATGGTTTAAATCATGTTTATATAAATAAAAAAATACCTAATGTTTCGAATATTAAAGATGTAAATGGTGTTTATTTGCCACAAGCTTTTGACATTTTTATTCAAATAAATCATTTTATCTCTGGGGGAATAAAACAAGTCCTATCATGA", "aa": "MSKQKQGRKAFLPKWAIFIIILAFILTAGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTFAKNNVQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVLS", "aa_hexdigest": "0c5d283c9664955eb0fefbcfaa2bb256", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 686852, "stop": 687671, "observations": { "insertion": [ 687629 ], "stop": [ 687157 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491E9D", "query_cov": 0.8864864864864865, "subject_cov": 0.4039408866995074, "identity": 0.945, "score": 314.0, "evalue": 2.55e-104, "gene_start": 22, "gene_end": 185, "reference_start": 8, "reference_end": 171, "reference_sequence": "MGNIYYNLGFYINGGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNIQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQSWSLQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQLKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSPIEYLMMN" }, "paralog": false, "identity": 0.9816176470588235, "score": 547.0, "evalue": 1.2e-198, "description": "Internal stop codon at 687157. Frameshift due to insertion around 687629.", "psc": { "uniref90_id": "UniRef90_UPI001E491E9D", "product": "MYPU_1760 family metalloprotease", "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491E9D", "UniRef:UniRef50_UPI001E491E9D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D" ], "product": "MYPU_1760 family metalloprotease" } }, "id": "LMJBGEANGM_1178", "locus": "GCA013368015_01178" }, { "type": "cds", "sequence": "contig_1", "start": 687782, "stop": 688234, "strand": "+", "frame": 2, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "COG:COG0742", "COG:J", "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "SO:0001217", "UniRef:UniRef50_A0A077L643", "UniRef:UniRef90_A0A454CAI7" ], "nt": "ATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAGTCAATAACAAGACCAACAATAGATAGAGCGAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGAGGCGCAAAATTCGCGGTATGTGTTGATAAAAATGCCGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGACTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTACTAGTTGAATGTTTAAACAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGA", "aa": "MLRIIAGKYRSRILKQPSKSITRPTIDRAREAIFSSIQFNIENKKVLDLFSGSGAFCLEALSRGAKFAVCVDKNAEAIKIIKENQNSLKEPNLKIFNIDALNFLNNNVEVFDYIYLDPPFVEKKLLVECLNKIIDKKCIDKSGTMIIETD", "aa_hexdigest": "b71f3fdbd296b995342c242285c41994", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAI7", "query_cov": 1.0, "subject_cov": 0.8152173913043478, "identity": 0.993, "score": 292.0, "evalue": 1.81e-99, "valid": true, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A077L643", "cog_id": "COG0742", "cog_category": "J", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L643" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "LMJBGEANGM_1179", "locus": "GCA013368015_01179" }, { "type": "cds", "sequence": "contig_1", "start": 688388, "stop": 689011, "strand": "+", "frame": 2, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0006163", "RefSeq:WP_012855758.1", "SO:0001217", "UniParc:UPI0001BC2503", "UniRef:UniRef100_D1J8S4", "UniRef:UniRef50_Q98PN5", "UniRef:UniRef90_D1J8S4" ], "nt": "ATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTATCAGTATCAATGACAACGAGATCACCACGAGAAGGAGAAATTGATGGTGTACACTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGTGCTAAACAAATTTTAGAATATTATAAAAAACGTAACAGATTGGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGCTATTCTACAATATTTCATCATGTGATAGTTAATGATGATCTAAATGTAGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAA", "aa": "MSKKLIIFTGPSGVGKGTIEKPLFEDKSLKLKLSVSMTTRSPREGEIDGVHYYFVSKDTFNSCLAENKLIEYSMHFDNYYGTLYSEIERISEQGKIPFLEIETNGAKQILEYYKKRNRLDEICSIFLMPPSYGELRKRIEGRNTETEDLIIKRMKKAKEEIGYSTIFHHVIVNDDLNVALEEIKEIIRSEFSDIINKKEVASEGRKA", "aa_hexdigest": "458f5bbf2abd014048df359e386016bd", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2503", "ncbi_nrp_id": "WP_012855758.1", "uniref100_id": "UniRef100_D1J8S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2503", "RefSeq:WP_012855758.1", "UniRef:UniRef100_D1J8S4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8S4", "uniref90_id": "UniRef90_D1J8S4" }, "psc": { "uniref90_id": "UniRef90_D1J8S4", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q98PN5", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN5" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "LMJBGEANGM_1180", "locus": "GCA013368015_01180" }, { "type": "cds", "sequence": "contig_1", "start": 689105, "stop": 689248, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTTTGTGTGATGGTATGGGCGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAACAATATTTCCTAGACACATTTCCTCAAAAGTTGGAATGTAGTGATAAAAAAAACGTTAGCAAATGA", "aa": "MAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSK", "aa_hexdigest": "897f954bd8244134008f6051081b9bdd", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5026.679600000001, "isoelectric_point": 7.717677879333496 }, "id": "LMJBGEANGM_1181", "locus": "GCA013368015_01181" }, { "type": "cds", "sequence": "contig_1", "start": 689333, "stop": 689770, "strand": "+", "frame": 2, "gene": "pTC1", "product": "Protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_D1J8S5", "UniRef:UniRef90_D1J8S5" ], "nt": "ATGGTTGTTGCCCTCATTTTTAATGCAAATGGTTTAGCTTATGTCTTTAATATTGGTGATTCACGCTTGTATGCATACAATGGATTACTTTATCAAATCACAGAAGATCAAAATTATTTATATCAGTTAATGAGAGAATTTAATTTAACATACGAAGAAGCAGCATTAGATCCTAATTCATACAAACTTATAAGTTGTCTAGGTCCCAATAAAAAAACCAATTGTCAGTCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAAGAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAA", "aa": "MVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE", "aa_hexdigest": "065e93331d98713cea8319b7fb60eda0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 689033, "stop": 689770, "observations": { "mutation": [ 689099, 689246 ], "stop": [ 689099, 689246 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S5", "query_cov": 1.0, "subject_cov": 0.5776892430278885, "identity": 1.0, "score": 284.0, "evalue": 2.29e-95, "gene_start": 1, "gene_end": 145, "reference_start": 107, "reference_end": 251, "reference_sequence": "MVDFCIASDQGKFRKENQDRAIVVNGEYWTLAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSKWFNNAISFIKEALVNYAKEYTEFEDMGTTMVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE" }, "paralog": false, "identity": 0.9918367346938776, "score": 484.0, "evalue": 8.34e-177, "description": "Internal stop codon at 689099, 689246. Nonsense mutation around 689099, 689246.", "psc": { "uniref90_id": "UniRef90_D1J8S5", "gene": "PTC1", "product": "Protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_D1J8S5", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S5", "EC:3.1.3.16", "UniRef:UniRef50_D1J8S5", "COG:COG0631", "COG:T", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S5" ], "product": "Protein phosphatase" } }, "id": "LMJBGEANGM_1182", "locus": "GCA013368015_01182" }, { "type": "cds", "sequence": "contig_1", "start": 689763, "stop": 690014, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00069.31", "PFAM:PF07714.23" ], "nt": "ATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTTGAATTAATTGGGACTGGAGGATTTGGCGAAGTTTATTCTGCTACATATAAAAAAAATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGTTGGACAGAGCAGAGAACGAGATTCATTAACGAAATAAACATATTAAAGGAAATTCATAGTTCTAACGTAGTAAAAATATTGGGCTGA", "aa": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKNNKRVAIKILNCIDDSRWTEQRTRFINEINILKEIHSSNVVKILG", "aa_hexdigest": "3129f318d6e5da1aabbfaa3dd80b3c2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00069.31", "name": "Pkinase", "length": 65, "aa_cov": 0.7831325301204819, "hmm_cov": 0.9692307692307692, "evalue": 1.6934123627246594e-13, "score": 46.0883674621582, "start": 18, "stop": 82 }, { "id": "PF07714.23", "name": "PK_Tyr_Ser-Thr", "length": 67, "aa_cov": 0.7590361445783133, "hmm_cov": 0.9552238805970149, "evalue": 9.441459206685791e-12, "score": 40.26829147338867, "start": 21, "stop": 83 } ], "seq_stats": { "molecular_weight": 9613.061799999996, "isoelectric_point": 9.37326793670654 }, "id": "LMJBGEANGM_1183", "locus": "GCA013368015_01183" }, { "type": "cds", "sequence": "contig_1", "start": 690045, "stop": 690779, "strand": "+", "frame": 3, "gene": "sPS1", "product": "mitogen-activated protein kinase kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "SO:0001217", "UniRef:UniRef50_D1J8S6", "UniRef:UniRef90_D1J8S6" ], "nt": "ATGGAGTTAATTAAAGGTCAGGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATCGATCCTGATACTGCAATTATGTATGCAAAGCAAATATGTCAAGGTTTGATTGAAATCCATGCTGCTAAAATAGTTCATAGAGATTTGAAACCAAGCAATATATTAATTGATGAAGAAACAAATAGCCTAAAATTAATTGATTTTGGTATTTCAATAGGTGAATCAACTCAACGTGTAACTAGAAATTATAAAGCTGTTGGTTCTGTTCAATATATGGCACCTGAATTATTGCGCGGAATAGCTGATCCTTCTCCAAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGAAAGGTTCCTTTTAATAACCATGATGATATTAACCAAATTATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCACAAGCATTAGAAAATATTGTAATTCGTTGCACCGCAAAATCTGCAGAGCAAAGATATACTGATTGCATCGAACTAATGCATGATTTAGAGACATGTTTAGATTCAAAAAGAGTAAATGAAAAAAGATTAGAATTAAATCCAAGTAAATCAAAAATGAAAGCCAAAAGAATCTTTAATAGTAAATTATTCAATATTATTTTTTTCACAACAGGAGGCTTTTTAGTAGCCTTTATTGTGATATTCGTGGTTTTATTTTTAAAGGGGATATTGTAG", "aa": "MELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNILIDEETNSLKLIDFGISIGESTQRVTRNYKAVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCIELMHDLETCLDSKRVNEKRLELNPSKSKMKAKRIFNSKLFNIIFFTTGGFLVAFIVIFVVLFLKGIL", "aa_hexdigest": "2ad03677e2f9bc1bbfbc628380854cc0", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "5-prime", "pseudogene": { "start": 689763, "stop": 690779, "observations": { "mutation": [ 690012 ], "stop": [ 690012 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S6", "query_cov": 1.0, "subject_cov": 0.7218934911242604, "identity": 0.996, "score": 468.0, "evalue": 4.51e-165, "gene_start": 1, "gene_end": 244, "reference_start": 95, "reference_end": 338, "reference_sequence": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKDNKRVAIKILNCIDDSRWTEQRTRFINEINILKEIHSSNVVKILGWHYKGNEVFYVMELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNILIDEETNSLKLIDFGISIGESTQRVTRNYKAVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCVELMHDLETCLDSKRVNEKRLELNPSKSKMKAKRIFNSKLFNIIFFTTGGFLVAFIVIFVVLFLKGIL" }, "paralog": false, "identity": 0.9911242603550295, "score": 665.0, "evalue": 3.68e-245, "description": "Internal stop codon at 690012. Nonsense mutation around 690012.", "psc": { "uniref90_id": "UniRef90_D1J8S6", "gene": "SPS1", "product": "mitogen-activated protein kinase kinase", "uniref50_id": "UniRef50_D1J8S6", "cog_id": "COG0515", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S6", "UniRef:UniRef50_D1J8S6", "COG:COG0515", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S6" ], "product": "mitogen-activated protein kinase kinase" } }, "id": "LMJBGEANGM_1184", "locus": "GCA013368015_01184" }, { "type": "cds", "sequence": "contig_1", "start": 690780, "stop": 691631, "strand": "+", "frame": 3, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "RefSeq:WP_175936879.1", "SO:0001217", "UniParc:UPI00158E52EA", "UniRef:UniRef100_UPI00158E52EA", "UniRef:UniRef50_D1J8S7", "UniRef:UniRef90_D1J8S7" ], "nt": "ATGATAGAAGGTAAAATAGTTAAATCGGTTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGAGGAAAACTTCGCTTGCTTGATATAGTGCCAATTGTGGGTGACAACGTTTTGTTTAAGAAAGACGAATTTATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCTTTGGTTGAACCTGAATTCAGTTCATTGCTCGTTGACAAATTTTTGATTATTATTGAAAGTAAGAATGTTAAACCTATTATCGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATTGCTTTTTATAAAGATCAAGGTTATGAAGTTTTTGAAATTAATTACGAAACAGGGCAAGGATTTGAAGGCTTAGGAGAAATATTTAGACATAAGACAAGCTTTTTTGTTGGTCAAACAGGTGTAGGAAAAACTACCTTGATAAATTATTTGGCAAAAACTAATTTTGAAACACAAGCAATTTCTAAAGCCTTAAACCGTGGAAAGCATACAACAAGAGAAGTTAATTTGATTGATTTTAATGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCCATAGAATTTGATTTAACAATTGAAGAACTACCAATTGCTTTTAAATCGTTTAGAGAAGCTGCAAGCAAATGTAAATTTAGAACAAGTTGTAAACATTATTTAGAATCCGTTGAGGATTGCGAAGTTAAAAAATTAGTTTTACAAGGCAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATCAAATAG", "aa": "MIEGKIVKSVAGFYDIKSFEDKQIYRVRGGGKLRLLDIVPIVGDNVLFKKDEFIYQILGRKNFFIRPKIANVDQAIVVMSLVEPEFSSLLVDKFLIIIESKNVKPIIVLTKKDLTSDSKIAFYKDQGYEVFEINYETGQGFEGLGEIFRHKTSFFVGQTGVGKTTLINYLAKTNFETQAISKALNRGKHTTREVNLIDFNGGEIIDTPGFSSIEFDLTIEELPIAFKSFREAASKCKFRTSCKHYLESVEDCEVKKLVLQGKIKQERYDNYISFLKRLTNPIK", "aa_hexdigest": "ee36727fdd8a62df4fa1a2a73c7e6011", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158E52EA", "ncbi_nrp_id": "WP_175936879.1", "uniref100_id": "UniRef100_UPI00158E52EA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E52EA", "RefSeq:WP_175936879.1", "UniRef:UniRef100_UPI00158E52EA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E52EA", "uniref90_id": "UniRef90_D1J8S7" }, "psc": { "uniref90_id": "UniRef90_D1J8S7", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_D1J8S7", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S7" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" }, "genes": [ "rsgA" ], "id": "LMJBGEANGM_1185", "locus": "GCA013368015_01185" }, { "type": "cds", "sequence": "contig_1", "start": 691745, "stop": 692287, "strand": "+", "frame": 2, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A448ZXM9", "UniRef:UniRef90_A0A454CAB1" ], "nt": "ATGGATGGAATTTTTGTAAACAATACTGCTTTAACATTTCAAGAAATCAAGCAAGTTAAAGAAAATTGTAAGAAACATACAATGGATATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCATTTGAAAATAGTGATCTTGAAAAGATGATTATGCAATGCCAAAAAGATAACGTTAAACTTGGCTTGGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGCTTTGGTGGCCAAAAATTTATTGAAAATTCATTTAATAAAATAAAGGAATTAGCAGACATTCGTAAGGCTTTAGATTTAAACTTTATTATTGAAGTGGATGGTGGAGTTAAAGACCAAAATATTGCCAAATGTTTTGAATGTGGCGTTAATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATTGACCAACTTTTAAAATAA", "aa": "MDGIFVNNTALTFQEIKQVKENCKKHTMDIHLMVKDVFKYYEMYKNIGDILTFHYEAFENSDLEKMIMQCQKDNVKLGLAIKPNTQVEEIRQYLKHCSLVLIMSVEPGFGGQKFIENSFNKIKELADIRKALDLNFIIEVDGGVKDQNIAKCFECGVNLAVVGSYLVKNFSKETIDQLLK", "aa_hexdigest": "fba67b4311482a66ecbde7c6b9d42d72", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAB1", "query_cov": 1.0, "subject_cov": 0.8372093023255814, "identity": 0.9940000000000001, "score": 361.0, "evalue": 6.64e-126, "valid": true, "gene": "Rpe", "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_A0A448ZXM9", "cog_id": "COG0036", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXM9" ], "product": "D-ribulose-5-phosphate 3 epimerase" }, "genes": [ "rpe" ], "id": "LMJBGEANGM_1186", "locus": "GCA013368015_01186" }, { "type": "cds", "sequence": "contig_1", "start": 692293, "stop": 693162, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9", "UniRef:UniRef90_D1J8S9" ], "nt": "ATGAAAAAAGCAAATAAAATATTACTGTCTACATTTGCAACTGCAATTTTAGCATTGCCTTTTGCCACAATTTCTTGTAAAGTCGATCAAATTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCAGCTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTAAAAACCCGTTTAGTAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTCTTGAAATTCAACTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAAGGAATTATTAAAATATTATAAATTAATGGCCTTGCTTCAAGATAAGTCTGAAGTAAATAAATACGCAGATTTAATAAAAAAATATTCAAAAAAATTTTCTGATGTTTTGAAGGATGATAGTTCAATATTAAATGATATTTACAATCAAGTTGCAAATTTAATTCCTAGAATTAATTTACTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATTCACTTTTTTGAATAATAATGGAATACTATTCAATTTGGATTTTATATTGAATAACATTGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAAAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTTTATAAAAATTATGAACTAGTTGAAGATTTTAATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGCAAATAATTATTTGGTTAATTGA", "aa": "MKKANKILLSTFATAILALPFATISCKVDQIKEKEKLINNSSQLNKENKKILLNELNILKTRLVNSKLNEKQKNEFLEIQLFLKLITLKELKISKELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDSSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLNNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFANNYLVN", "aa_hexdigest": "cf23901a14b6683b3225ba83a745e192", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 692293, "stop": 693414, "observations": { "mutation": [ 693160 ], "stop": [ 693160 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S9", "query_cov": 1.0, "subject_cov": 0.774798927613941, "identity": 0.9790000000000001, "score": 529.0, "evalue": 8.5e-188, "gene_start": 1, "gene_end": 289, "reference_start": 1, "reference_end": 289, "reference_sequence": "MKKANKILLSTFATTILALPFATISCKVDQVKEKEKLINNSSQLNKENKKILLNELNILKTRLVNSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDSSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLSNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFVNNYLVNWLTNEPNICQIGEDKFLIYYHLPFSIENKKIVQKPSLYISNVVADSTIIKAIASRNVYLLKNLFLLLPKENYNENLKVLNNLMK" }, "paralog": false, "identity": 0.9785522788203753, "score": 716.0, "evalue": 3.09e-264, "description": "Internal stop codon at 693160. Nonsense mutation around 693160.", "psc": { "uniref90_id": "UniRef90_D1J8S9", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S9", "UniRef:UniRef50_D1J8S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9" ], "product": "Lipoprotein" } }, "id": "LMJBGEANGM_1187", "locus": "GCA013368015_01187" }, { "type": "cds", "sequence": "contig_1", "start": 693812, "stop": 694351, "strand": "+", "frame": 2, "gene": null, "product": "Lmp related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8T0", "UniRef:UniRef50_UPI001939F067", "UniRef:UniRef90_D1J8T0" ], "nt": "ATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCATATTATGTGGAGGTTTAGCGGTGGCATCAATTGCTACAGTTGCTGCATACGCATGTAAATCAAGATCAGAATCAAAAATAGAAAAAGAAAATCTTTTGAATGAACTTAAAGAGGCATTAAATCAATTAAAACAATCGATTGCATCACCAGAAGCTAAAAATATTGAAAAATCAATGGAAATCAATATCTTAAATAATGCTAATGTTTCAAAAGAATCCTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAACAAGGAATTTGAAAAATTTGAACAAATCAAAAACGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATCGAAATTAAATAACATATCGCAAAGCTCAAAAGAACAAGAAATTATAGACGCAATAAGCAAATTGTATGATTTAAAGAAATGA", "aa": "MYKKTNRKILISLAILCGGLAVASIATVAAYACKSRSESKIEKENLLNELKEALNQLKQSIASPEAKNIEKSMEINILNNANVSKESSIKEIQSKINQVKYAIDSLNKKIADRNNKEFEKFEQIKNDLQTYINRDLNVVIYNEITKKSKNEISKLNNISQSSKEQEIIDAISKLYDLKK", "aa_hexdigest": "51f7ea9b90ce00a3adbffa84d212c16e", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001939F067", "query_cov": 1.0, "subject_cov": 0.8731707317073171, "identity": 0.8320000000000001, "score": 223.0, "evalue": 2.07e-71, "valid": false, "uniref50_id": "UniRef50_UPI001939F067" }, "pscc": { "uniref50_id": "UniRef50_UPI001939F067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F067" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 693812, "stop": 694654, "observations": { "mutation": [ 694349 ], "stop": [ 694349 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8T0", "query_cov": 1.0, "subject_cov": 0.5507692307692308, "identity": 0.866, "score": 228.0, "evalue": 9.49e-72, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MYKKTNRKILISLAILCGGLAVASIATVAAYAGKSKPESKIEKENLLNELKEALNQLKQLIASPEAKNIEKSMKINILNNANVSKDSSIKEIQSKINQVKYAINSLNKKIADRNKKEFEKFEQIKNDLQTYINRDLNVVIYNEITKKSKNEILKLNNISQSSKEQEIIDAISKLYDLKKWILAQKLLIDNSINEQKRQLKSLIKIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTTSELFRERILLHKCINETLLFEETMFKEKETKINELKKQSETIFEHIGKDPNNSIFDYNSQFYRNIITKIDSLNTNEKSLTLNKKI" }, "paralog": false, "identity": 0.9, "score": 428.0, "evalue": 4.96e-153, "description": "Internal stop codon at 694349. Nonsense mutation around 694349.", "psc": { "uniref90_id": "UniRef90_D1J8T0", "product": "Lmp related protein", "uniref50_id": "UniRef50_D1J8T0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8T0", "UniRef:UniRef50_D1J8T0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8T0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8T0" ], "product": "Lmp related protein" } }, "id": "LMJBGEANGM_1188", "locus": "GCA013368015_01188" }, { "type": "cds", "sequence": "contig_1", "start": 694439, "stop": 694918, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E", "UniRef:UniRef90_UPI001594B72E" ], "nt": "TTGTTATCAAACAAAATAGTTGAACGTGACCTAGAATTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAACAAAACTGCATCTGAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCTATTATTTGAAGAAACAATGTTTAAAGAAAAGGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACAATTTTTGAACATATTGGAAAAGACCCAAATAATAGCATATTCGATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACACTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGTAATGAAATAAATAATCTAGACAAATCATTTTCTAAGTTGGTTTCTGATACCCTTGAAGAATTTGAATATGAGGATGATCTTAAAGGCCATATTGATGTTGCAAAAGATCCCAAAAATTGA", "aa": "MLSNKIVERDLEFKQEISKAEASLSQTNKTASELFRERILLHKFINETLLFEETMFKEKETKINELKKQSETIFEHIGKDPNNSIFDYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEEFEYEDDLKGHIDVAKDPKN", "aa_hexdigest": "1a22901359a66005abfd9b430d33a081", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 694271, "stop": 694958, "observations": { "insertion": [ 694889 ], "mutation": [ 694349 ], "stop": [ 694349 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001594B72E", "query_cov": 0.9937106918238994, "subject_cov": 0.6899563318777293, "identity": 0.9179999999999999, "score": 264.0, "evalue": 1.58e-87, "gene_start": 1, "gene_end": 158, "reference_start": 58, "reference_end": 215, "reference_sequence": "MKLNSISQSSKEQEIIDAISKLYDLKKWILAQKLLIDNSINEQKRQLKSLIEIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTTSELFRERILLHKFINETLLLEEKMLKEKETKINELKKQSETIFEHIGKDPNNSIFYYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEKFEYEDDLKDHMMLQKIPKIELIFKNISDTLGQ" }, "paralog": false, "identity": 0.9473684210526315, "score": 405.0, "evalue": 1.44e-145, "description": "Internal stop codon at 694349. Frameshift due to insertion around 694889. Nonsense mutation around 694349.", "psc": { "uniref90_id": "UniRef90_UPI001594B72E", "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001594B72E", "UniRef:UniRef50_UPI001594B72E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18670.72719999999, "isoelectric_point": 5.042494010925293 }, "id": "LMJBGEANGM_1189", "locus": "GCA013368015_01189" }, { "type": "cds", "sequence": "contig_1", "start": 695201, "stop": 695428, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "ATGCAAGTAGACCAAATAACAGACGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTTTGAAAGCAAACTATTAAGTGCAATATTGTTGCCTACAATATTTAAAAAGCCATATGAAAATCTTTCTAAAATAGTAAAGCCAATTATAGATATGATACATAATAAAGACGAATATAGCATAGAGATTATTGGATGA", "aa": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIG", "aa_hexdigest": "798d9665491a9955e8927b63f637e1ad", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 695201, "stop": 695731, "observations": { "mutation": [ 695426 ], "stop": [ 695426 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 1.0, "score": 142.0, "evalue": 1.12e-41, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9659090909090909, "score": 322.0, "evalue": 3.63e-115, "description": "Internal stop codon at 695426. Nonsense mutation around 695426.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8709.233199999995, "isoelectric_point": 5.721890449523926 }, "id": "LMJBGEANGM_1190", "locus": "GCA013368015_01190" }, { "type": "cds", "sequence": "contig_1", "start": 695510, "stop": 695737, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "TTGACACAAGAAAATTTAAAAAATAAATTAAAAGAATTTAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAAGCATCCGAAAAAGGCATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAGTTCTATTATTAGAATAAAAAAAGAAATAAGATATCTTGAATATAAACTAGATTAA", "aa": "MTQENLKNKLKEFKLVKENLGNLIKKASEKGIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLEYKLD", "aa_hexdigest": "c9404622bd34d607bf244dce9cd721f2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 695210, "stop": 695737, "observations": { "mutation": [ 695426 ], "stop": [ 695426 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 0.96, "score": 133.0, "evalue": 4.93e-38, "gene_start": 1, "gene_end": 75, "reference_start": 104, "reference_end": 178, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9657142857142857, "score": 321.0, "evalue": 1.48e-114, "description": "Internal stop codon at 695426. Nonsense mutation around 695426.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8944.086999999992, "isoelectric_point": 5.102345466613768 }, "id": "LMJBGEANGM_1191", "locus": "GCA013368015_01191" }, { "type": "cds", "sequence": "contig_1", "start": 696170, "stop": 696487, "strand": "-", "frame": 2, "gene": null, "product": "leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A4608", "UniRef:UniRef90_UPI00280AAAA0" ], "nt": "TTGCCAAAAAATATTGTTGGTTTTGGTCGAGATGTGTTTAATTCAGATTGCAAAATAATCTTAGATAGCAATATAACATGCATTTCTCGTGGCATAGCAAAAGCCTTTGAAAATAGTAAAAAAGTAATTATTCCAAATTTTGTTAAAGAAATCGAAGAAGGCGCATTTGAGGGTTTCAAAAATCTAAAAGAAGTAGTTTTAAATGAAGGAATTGAAAGAATTGGTAGCTATGCTTTTAGAAACCCAAGACTAGAAGTAGTAACTATTCCAAATTCAGTTAAAGAAATTGGAACTAATGCGTTTTTAGCTGGAGTTTGA", "aa": "MPKNIVGFGRDVFNSDCKIILDSNITCISRGIAKAFENSKKVIIPNFVKEIEEGAFEGFKNLKEVVLNEGIERIGSYAFRNPRLEVVTIPNSVKEIGTNAFLAGV", "aa_hexdigest": "49e36e387379057217e60bafb878aeee", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 696044, "stop": 696601, "observations": { "mutation": [ 696088, 696094, 696172 ], "stop": [ 696088, 696094, 696172 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280AAAA0", "query_cov": 1.0, "subject_cov": 0.5675675675675675, "identity": 0.981, "score": 135.0, "evalue": 3.57e-38, "gene_start": 1, "gene_end": 105, "reference_start": 39, "reference_end": 143, "reference_sequence": "MEEGAFRNFKELKEVKLNEGLKSIGNYAFLGTSISSVVLPKNIVGFGRDVFNSDCKIILDSNITCISRGIAKAFENSKKVIIPNFVKEIEEGAFEGFENLKEVVLNEGIERIGSYAFRNPRLEVVTIPNSVKEIGTNAFLAGVWNNNVIRSLIPSLKEVYYKGNKNNTPWNWKDIGIDIKIVKEI" }, "paralog": false, "identity": 0.9567567567567568, "score": 272.0, "evalue": 1.12e-94, "description": "Internal stop codon at 696088, 696094, 696172. Nonsense mutation around 696088, 696094, 696172.", "psc": { "uniref90_id": "UniRef90_UPI00280AAAA0", "product": "leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_UPI00193A4608", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280AAAA0", "UniRef:UniRef50_UPI00193A4608" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A4608", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A4608" ], "product": "leucine-rich repeat domain-containing protein" } }, "id": "LMJBGEANGM_1192", "locus": "GCA013368015_01192" }, { "type": "cds", "sequence": "contig_1", "start": 696942, "stop": 697289, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8B9B7", "UniRef:UniRef90_UPI0005C8B9B7" ], "nt": "TTGCTAAAATTAATCTTCTTTAAATATCAACAATCCTTAAAGGTTTTTGCCTTAATTTCTTTAATTGAACTAGGAATAGAAATTGAAGAGATTTGTGTGCCTTCAAATGCACCTTCTCCAATATTTTCAATACCATTTTTTAAAGTTAATTCCTTTAGATTTTTAAAGTTTTTAAAGGCTTCATTTTCTATTGATTTTACATTTTTGGGAATAGTAACAGAGGCAATTTTTTTAAGTCCCTTTGTTAAATCTTTAGTAATTTTGTCAAAACCATTATCAAATTTTATTTTGCAATTTTCAAAAGAAGCATTTTCATCTATTTCCTCTACCTTTTTGCCTTTAAAATAG", "aa": "MLKLIFFKYQQSLKVFALISLIELGIEIEEICVPSNAPSPIFSIPFFKVNSFRFLKFLKASFSIDFTFLGIVTEAIFLSPFVKSLVILSKPLSNFILQFSKEAFSSISSTFLPLK", "aa_hexdigest": "3f6e920b4a49867a665471f9a9b51be2", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0005C8B9B7", "query_cov": 1.0, "subject_cov": 0.9426229508196722, "identity": 0.93, "score": 192.0, "evalue": 1.16e-61, "valid": true, "uniref50_id": "UniRef50_UPI0005C8B9B7" }, "pscc": { "uniref50_id": "UniRef50_UPI0005C8B9B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005C8B9B7" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13041.469599999982, "isoelectric_point": 9.416977500915529 }, "id": "LMJBGEANGM_1193", "locus": "GCA013368015_01193" }, { "type": "cds", "sequence": "contig_1", "start": 697337, "stop": 697609, "strand": "-", "frame": 2, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019398118" ], "nt": "ATGTTATTAACTTTAGGGCTTGTTACAACTTCAACAATTTGCTTTCCATTAGTAGCGGCATCATGTACTAAAAAAACAGACAGCAATTCAAACAATCAAAATATAGATAATAATCCTACAAAGAATAATGACAACAAACCTAATGACAAAAAGCCAAAATCGTTAAAGAATGAGGATTCTTCTGCAAATAACGAAAATCAAAATGTTCAAGATAATAAAGAAAAAGAATTATATGAACTTTTTGATTTTGAAAGAAATTCTTCAAATAATTGA", "aa": "MLLTLGLVTTSTICFPLVAASCTKKTDSNSNNQNIDNNPTKNNDNKPNDKKPKSLKNEDSSANNENQNVQDNKEKELYELFDFERNSSNN", "aa_hexdigest": "f157e0921e3d4ec777e26df20198d295", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0019398118", "query_cov": 1.0, "subject_cov": 0.9473684210526315, "identity": 0.8, "score": 80.1, "evalue": 7.81e-18, "valid": false, "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI0019398118" }, "pscc": { "uniref50_id": "UniRef50_UPI0019398118", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0019398118" ], "product": "variable surface lipoprotein" }, "genes": [], "id": "LMJBGEANGM_1194", "locus": "GCA013368015_01194" }, { "type": "cds", "sequence": "contig_1", "start": 697951, "stop": 698565, "strand": "+", "frame": 1, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "nt": "ATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTAGCTTTTAAAAATGGCGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTTTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATTACAGTTCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAAAACGCAGGAATTATTGCTGGTCTTGATGTTGTAAGAATTATTAACGAACCTACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCGTTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTAGCAACAGCAGGCGATAACCACTTGGGTGGTGATGACTGA", "aa": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDD", "aa_hexdigest": "8ddb54cc4cdd33d560f0071fd07de60e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1EEFC2", "query_cov": 0.9754901960784313, "subject_cov": 0.9660194174757282, "identity": 0.6729999999999999, "score": 264.0, "evalue": 4.31e-87, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A3D2IMT5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D2IMT5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "LMJBGEANGM_1195", "locus": "GCA013368015_01195" }, { "type": "cds", "sequence": "contig_1", "start": 698587, "stop": 698862, "strand": "+", "frame": 1, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "nt": "ATGGTTGAACAAATTAAATCTAAATACAATTTCGATCCAACAACTGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACTCGTGCTACATTTGAATCAATGACTGAGCATTTACTACAAAGAACAAGAAAACCATTATTAGATGTTTTAAGTGAAGCAAAATTAACATGA", "aa": "MVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTRKPLLDVLSEAKLT", "aa_hexdigest": "31f1a5a70ac54c463adff44b9808218f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B89B0", "query_cov": 0.9340659340659341, "subject_cov": 0.8252427184466019, "identity": 0.588, "score": 106.0, "evalue": 3.29e-28, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A223M9D4" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "LMJBGEANGM_1196", "locus": "GCA013368015_01196" }, { "type": "cds", "sequence": "contig_1", "start": 698878, "stop": 699738, "strand": "+", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_Q9ZEJ0" ], "nt": "GTGTTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAACTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCAGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGATGTTACTCCTTTAACATTAGGTATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATCGTTATCACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTCAATCTTGAAGGAATTGAACCTGCACCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGATAAAAAGACAAACAAAGAACAAACAATAACCATTCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGACGCAGAAGCAAATAGAGAAGCTGATCAAAAGAAACGCCACGAAATTGAAGTTATTGTAAAAGCAGAACAATTAAGCAATGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAATCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGAAATAGATGAAATTAAAGAATTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAACGACAACCCTCAAACTAACTAA", "aa": "MLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN", "aa_hexdigest": "21c036ad0da62f0e522370c355b68e2d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_W6TIT3", "query_cov": 0.9755244755244755, "subject_cov": 0.8584615384615385, "identity": 0.509, "score": 243.0, "evalue": 6.33e-76, "valid": false, "product": "Chaperone protein dnaK", "uniref50_id": "UniRef50_W6TIT3" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 698578, "stop": 699738, "observations": { "mutation": [ 698584, 698860 ], "stop": [ 698584, 698860 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZEJ0", "query_cov": 0.9965034965034965, "subject_cov": 0.4789915966386555, "identity": 1.0, "score": 509.0, "evalue": 9.15e-177, "gene_start": 2, "gene_end": 286, "reference_start": 311, "reference_end": 595, "reference_sequence": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDDWDHEIVKWMVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTRKPLLDVLSEAKLTWNDINEVLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN" }, "paralog": false, "identity": 0.9948186528497409, "score": 730.0, "evalue": 3.27e-266, "description": "Internal stop codon at 698584, 698860. Nonsense mutation around 698584, 698860.", "psc": { "uniref90_id": "UniRef90_Q9ZEJ0", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZEJ0", "UniRef:UniRef50_Q49539", "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "LMJBGEANGM_1197", "locus": "GCA013368015_01197" }, { "type": "cds", "sequence": "contig_1", "start": 699959, "stop": 700261, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTAATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATAATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATCTGA", "aa": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAII", "aa_hexdigest": "13c2c510db0c8a1b34ed134faf46f4eb", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11355.716099999987, "isoelectric_point": 9.781288337707515 }, "id": "LMJBGEANGM_1198", "locus": "GCA013368015_01198" }, { "type": "cds", "sequence": "contig_1", "start": 700304, "stop": 700717, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B", "UniRef:UniRef90_UPI001E50136B" ], "nt": "TTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGGCATTGGTTGCACATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATCATAATTTTTGCACCTATGGCAATAGGTGGCGGATTTATATTTGCCAACTCAACATCAACAAATGAAAATTTTGCTCATTATATTAATAGCAAATATAAATACCATAGAGCAGGAAATGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCCTGA", "aa": "MFAFVSSIIVYSQVSKGNMFGYLVLLALVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLA", "aa_hexdigest": "1250f1d1d8da8eb3e0a3ac646024dc7c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 700004, "stop": 700845, "observations": { "deletion": [ 700835 ], "mutation": [ 700259, 700298, 700715, 700760 ], "stop": [ 700259, 700298, 700715, 700760 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E50136B", "query_cov": 1.0, "subject_cov": 0.46440677966101696, "identity": 0.985, "score": 259.0, "evalue": 5.31e-85, "gene_start": 1, "gene_end": 137, "reference_start": 116, "reference_end": 252, "reference_sequence": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAIIWGKILSFNSLGLIWTLFAFVSSIIVYSQVSKGNMFGYLVLLSLVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLAWKYSNSSANEWQKYSWLAMPYQFVDIFNIENQNIFSNLSSRQY" }, "paralog": false, "identity": 0.9821428571428571, "score": 530.0, "evalue": 3.27e-194, "description": "Internal stop codon at 700259, 700298, 700715, 700760. Frameshift due to deletion around 700835. Nonsense mutation around 700259, 700298, 700715, 700760.", "psc": { "uniref90_id": "UniRef90_UPI001E50136B", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E50136B", "UniRef:UniRef50_UPI001E50136B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B" ], "product": "ABC transporter permease" } }, "id": "LMJBGEANGM_1199", "locus": "GCA013368015_01199" }, { "type": "cds", "sequence": "contig_1", "start": 700769, "stop": 701131, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATATCAATTTGTTGATATATTCAACATTGAAAATCAAAATATTTTTAGTAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAACTAAGATTAATAAATACTATGTCCCAGGGATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCTTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGA", "aa": "MPYQFVDIFNIENQNIFSNLSSDSINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLK", "aa_hexdigest": "b6aa917564e8c4b1a5a800e5c70bec1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13912.351099999982, "isoelectric_point": 7.826155662536623 }, "id": "LMJBGEANGM_1200", "locus": "GCA013368015_01200" }, { "type": "cds", "sequence": "contig_1", "start": 701138, "stop": 701725, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247", "UniRef:UniRef90_UPI001E5A3247" ], "nt": "ATGAGCCAAGTTTTAAAATATCATAAATTTACAGAAGCAGCCGAAGAATTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCACATGATATTAAAAAGTTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTGCAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCGTTGCTATATTTCATCTATTTTAAAGATAATCAAGGTCAAGTTATTAAATCATTATTGAAATCTGAAAGCGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTGCAAGAAAATAAAATAACTAAAAATAAACAAGTTGTAGTTAGATATGAATTAATTCCAAGTAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGA", "aa": "MSQVLKYHKFTEAAEEFYSKVKNEISLKKLVKPHDIKKLLLETISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILI", "aa_hexdigest": "1714de7b6c4bf654923db67fee8a3efd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 700838, "stop": 701791, "observations": { "mutation": [ 701129, 701723 ], "stop": [ 701129, 701723 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5A3247", "query_cov": 1.0, "subject_cov": 0.5571428571428572, "identity": 0.985, "score": 369.0, "evalue": 8.5e-127, "gene_start": 1, "gene_end": 195, "reference_start": 134, "reference_end": 328, "reference_sequence": "MNDKNIHDLQCHINLLIYSTLKIKIFLVTFLRDNINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLKWKIMSQVLKYHKFIEAAEEFYSKVKNEISLKKLVKPYDIKKLLLEMISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILIWIVVAIILMMANRFLYLRKDYK" }, "paralog": false, "identity": 0.9779179810725552, "score": 612.0, "evalue": 6.2e-225, "description": "Internal stop codon at 701129, 701723. Nonsense mutation around 701129, 701723.", "psc": { "uniref90_id": "UniRef90_UPI001E5A3247", "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5A3247", "UniRef:UniRef50_UPI001E5A3247" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 22894.838900000006, "isoelectric_point": 8.76139850616455 }, "id": "LMJBGEANGM_1201", "locus": "GCA013368015_01201" }, { "type": "cds", "sequence": "contig_1", "start": 701791, "stop": 702495, "strand": "+", "frame": 1, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_020002508.1", "SO:0001217", "UniParc:UPI000365E072", "UniRef:UniRef100_A0A454CAA1", "UniRef:UniRef50_A0A449B6T2", "UniRef:UniRef90_D1J8T4" ], "nt": "ATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGCGAAAATGGTGCAGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGCGGAATTACAAACTTAAACCCTAATTCTAAAAAAATTCTTGGTTATGTTCCAGAAAATGCAGTGTTTCCTAAAGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCGGGGATAAATAAAACCGAAGTAAAGGCAAAAATTGAAAGTTATTTACAAAGATTTGGAATTATTGAATTAAAAAACGAAAAACCCGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAAGCTTTAATTAACGAGCCTAAATTATTGATTTTAGATGAACCAGCTGCTAATTTGGACCCTACTGCAAGATTTGAATTGTTTTCAATATTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCGTTGACATTAATTCATGGTGGAAAGATCTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAG", "aa": "MDNHILELKNVVKIYNKSDRGIHNISFAVESGNFHAFIGENGAGKTTTIKTIIGSYINFKGEILIGGITNLNPNSKKILGYVPENAVFPKELTTYEYLKALALLSGINKTEVKAKIESYLQRFGIIELKNEKPANFSSGQKKKVLLIQALINEPKLLILDEPAANLDPTARFELFSILHELNEKGTTIFISSHVLSEIDKYANSLTLIHGGKILYSGPKYDSLEKIFYEKVIKN", "aa_hexdigest": "cbe68b00230783e80869ce26faa8a7ac", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000365E072", "ncbi_nrp_id": "WP_020002508.1", "uniref100_id": "UniRef100_A0A454CAA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000365E072", "RefSeq:WP_020002508.1", "UniRef:UniRef100_A0A454CAA1" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CAA1", "uniref90_id": "UniRef90_D1J8T4" }, "psc": { "uniref90_id": "UniRef90_D1J8T4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A449B6T2", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6T2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6T2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "LMJBGEANGM_1202", "locus": "GCA013368015_01202" }, { "type": "cds", "sequence": "contig_1", "start": 702473, "stop": 702865, "strand": "+", "frame": 2, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35", "UniRef:UniRef90_UPI000A573B35" ], "nt": "ATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCGTTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATTGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATCCAAGCAAAAATTAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGACATTTTTGTACAAAACCAAAATAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTTCCTATTTATAATAGAGCCTCAGATAATCCAGGTAGCGAACTTTATGGATTTATAAGGCAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGA", "aa": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRN", "aa_hexdigest": "bf92365f4129e4aec179e2b3aa3b5ef9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 702473, "stop": 702998, "observations": { "insertion": [ 702947 ], "mutation": [ 702863, 702869 ], "stop": [ 702863, 702869 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A573B35", "query_cov": 1.0, "subject_cov": 0.7471264367816092, "identity": 1.0, "score": 243.0, "evalue": 9.04e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRNWYWYLKQIGNFVYSINPYNDRYIETSQTKKQFNLPKKTLVFYIK" }, "paralog": false, "identity": 0.9827586206896551, "score": 330.0, "evalue": 3.12e-117, "description": "Internal stop codon at 702863, 702869. Frameshift due to insertion around 702947. Nonsense mutation around 702863, 702869.", "psc": { "uniref90_id": "UniRef90_UPI000A573B35", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A573B35", "UniRef:UniRef50_UPI000A573B35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35" ], "product": "aromatic motif membrane protein" } }, "id": "LMJBGEANGM_1203", "locus": "GCA013368015_01203" }, { "type": "cds", "sequence": "contig_1", "start": 703007, "stop": 703402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE", "UniRef:UniRef90_UPI000A5C6EDE" ], "nt": "ATGCCTAAGGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTTGCTAAAAAAATTAATGAAAGTATTGATTCTAAAGTTCAAAACCAATTTAGCGATTATTTAGAAGATAGAATTGATGAATCATCGAAGTTAAAAATAGATACAAATTCAGATATAACCAATTTTTTAAAAAGCAAAATTAACTTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAATAATAAGAACAAATTTGAAATTAGTAATCAATATCAAATTAATGATGAAATTTCTAATAAAATATTTAATCTTTTAAATACAAAAGGATCTTCATTTGACAACTATGCTTCAAAGAAAATCTTCTACGTAGCATACGACCACAAAACTTGA", "aa": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDTNSDITNFLKSKINLNDAKKLIENYLNNKNKFEISNQYQINDEISNKIFNLLNTKGSSFDNYASKKIFYVAYDHKT", "aa_hexdigest": "10ec2da999b7df35803b7a749cc0ec9a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 703007, "stop": 703705, "observations": { "mutation": [ 703400 ], "stop": [ 703400 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "query_cov": 1.0, "subject_cov": 0.5282258064516129, "identity": 0.9390000000000001, "score": 234.0, "evalue": 3.42e-76, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 131, "reference_sequence": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDINSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNEYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKTWIRVIVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE" }, "paralog": false, "identity": 0.9612068965517241, "score": 441.0, "evalue": 3.47e-160, "description": "Internal stop codon at 703400. Nonsense mutation around 703400.", "psc": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C6EDE", "UniRef:UniRef50_UPI000A5C6EDE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15265.925599999982, "isoelectric_point": 5.46065845489502 }, "id": "LMJBGEANGM_1204", "locus": "GCA013368015_01204" }, { "type": "cds", "sequence": "contig_1", "start": 703544, "stop": 703753, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCAATTATTGATTCTTTAATTGAAAATATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAA", "aa": "MNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE", "aa_hexdigest": "bcfa9f81827cb6780bcbbaa8e8746bb1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8619.6525, "isoelectric_point": 5.142019081115721 }, "id": "LMJBGEANGM_1205", "locus": "GCA013368015_01205" }, { "type": "cds", "sequence": "contig_1", "start": 703746, "stop": 704051, "strand": "+", "frame": 3, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1", "UniRef:UniRef90_UPI00241181B1" ], "nt": "ATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACTGCTATATCGTGCGTTGATAAAAGCGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGGGACGTTGTAAATAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAACGACTTATTAAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTGGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGA", "aa": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKK", "aa_hexdigest": "670d981eccc8d6508d2c5805232c3907", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 703746, "stop": 704354, "observations": { "mutation": [ 704049, 704121, 704172 ], "stop": [ 704049, 704121, 704172 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241181B1", "query_cov": 1.0, "subject_cov": 0.4855769230769231, "identity": 1.0, "score": 185.0, "evalue": 1.13e-57, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKKWNSKLNNTEKENIANQLNQIYEKNWYIVLTNINKFNLRFYEWLLFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLRELKEGDESGELSNMNMLYLKKR" }, "paralog": false, "identity": 0.9702970297029703, "score": 383.0, "evalue": 2.52e-138, "description": "Internal stop codon at 704049, 704121, 704172. Nonsense mutation around 704049, 704121, 704172.", "psc": { "uniref90_id": "UniRef90_UPI00241181B1", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241181B1", "UniRef:UniRef50_UPI00241181B1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1" ], "product": "aromatic motif membrane protein" } }, "id": "LMJBGEANGM_1206", "locus": "GCA013368015_01206" }, { "type": "cds", "sequence": "contig_1", "start": 704178, "stop": 704441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAAACCTGAATTTTTAAGTCAAATTAGAGCTGATGGAAAACATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAGAAACCAGATAATGTTCCAATTATCAATAATTATTTAAAGGAATTAAAAGAAGGTGATGAATCAGGGGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTAACAAAACCAAAAATTAAACTAACGTGTTTTGTGTGA", "aa": "MFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLKELKEGDESGELSNMNMLYLKKDSMIFRFRVVDTLTKPKIKLTCFV", "aa_hexdigest": "c03b67fc41a90cbb416fdcf9376ff96d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10187.76769999999, "isoelectric_point": 9.01450138092041 }, "id": "LMJBGEANGM_1207", "locus": "GCA013368015_01207" }, { "type": "cds", "sequence": "contig_1", "start": 704475, "stop": 704639, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAGATATTGTCCATAGTGCTTATATTCACGGAGTTCAAGAAGGATTTAATAGATTTGAAAACGATATGGCAAATCGTTATGGAAAACCCGCCAAAATGATCATGGGAATTTTCGATGATAATGCTTCTAGTGCCAATGGAGGAAAAGAAAATGAATAA", "aa": "MISDIVHSAYIHGVQEGFNRFENDMANRYGKPAKMIMGIFDDNASSANGGKENE", "aa_hexdigest": "6afceb24d95b2ddc41a50030defe4c38", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5978.556100000002, "isoelectric_point": 5.140711784362792 }, "id": "LMJBGEANGM_1208", "locus": "GCA013368015_01208" }, { "type": "cds", "sequence": "contig_1", "start": 704596, "stop": 704790, "strand": "+", "frame": 1, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388", "UniRef:UniRef90_UPI001E405388" ], "nt": "ATGATAATGCTTCTAGTGCCAATGGAGGAAAAGAAAATGAATAAAAAAATTTTGATGACTCTATCATTGAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATTGAGGGCAACGAAAATGCAAACGAAAGAGAATGA", "aa": "MIMLLVPMEEKKMNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANERE", "aa_hexdigest": "21146ce2d0741f7348732be854b7152e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 704632, "stop": 704889, "observations": { "mutation": [ 704788 ], "stop": [ 704788 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E405388", "query_cov": 0.8125, "subject_cov": 0.6046511627906976, "identity": 1.0, "score": 96.3, "evalue": 8.78e-25, "gene_start": 13, "gene_end": 64, "reference_start": 1, "reference_end": 52, "reference_sequence": "MNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANEREWKNFLNRDYVKEILRLSFDADVDAKNKYIAEQKN" }, "paralog": false, "identity": 0.9647058823529412, "score": 160.0, "evalue": 1.33e-52, "description": "Internal stop codon at 704788. Nonsense mutation around 704788.", "psc": { "uniref90_id": "UniRef90_UPI001E405388", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E405388", "UniRef:UniRef50_UPI001E405388" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388" ], "product": "aromatic motif membrane protein" } }, "id": "LMJBGEANGM_1209", "locus": "GCA013368015_01209" }, { "type": "cds", "sequence": "contig_1", "start": 705106, "stop": 705312, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGAATCATCAGATGAACAATCAGCATTAACTGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAGGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGAGAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTCTTCAATTAACTATTGATTGA", "aa": "MNKFESSDEQSALTAQGNSLILGHFYKPRSNEIIQYTTQVTKNDKNGERTYDVYLLTKEGFILQLTID", "aa_hexdigest": "6c52ebae7d98fae9c42e6bfd9dff01b4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7817.6195, "isoelectric_point": 5.216534996032714 }, "id": "LMJBGEANGM_1210", "locus": "GCA013368015_01210" }, { "type": "cds", "sequence": "contig_1", "start": 705855, "stop": 706022, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGAAATAATAGGAAGAACAACAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGA", "aa": "MLEIIGRTTKKQRKIMSEQKQQAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQ", "aa_hexdigest": "7461acaa8e4de47d38c08816005aa396", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6180.082899999999, "isoelectric_point": 9.989134407043458 }, "id": "LMJBGEANGM_1211", "locus": "GCA013368015_01211" }, { "type": "cds", "sequence": "contig_1", "start": 706059, "stop": 706160, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGA", "aa": "MGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFI", "aa_hexdigest": "82e2324bb86e16d48affdc20ce88ce1b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3821.5944, "isoelectric_point": 9.602904319763184 }, "id": "LMJBGEANGM_1212", "locus": "GCA013368015_01212" }, { "type": "cds", "sequence": "contig_1", "start": 706359, "stop": 706799, "strand": "+", "frame": 3, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "COG:COG0052", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_D5E5E7", "UniRef:UniRef90_D1J8T8" ], "nt": "ATGGACAAGAAAATTGCTAAATTGCAAAAGAACTTAGGTGGTATTAGAAAATTAGCTGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCAGCAAACGATGATTCTGTAAAGTCAATTACTTTAATTACAACTATCTTGGCAGACGCAATAGCATCAGCACATGGTGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACTGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATCAGAAAATACTAAGAAATCTGAATAA", "aa": "MDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE", "aa_hexdigest": "3faafe1bfebc59bb844b5f86d14e5d0f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 706059, "stop": 706799, "observations": { "mutation": [ 706158, 706239 ], "stop": [ 706158, 706239 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8T8", "query_cov": 1.0, "subject_cov": 0.48344370860927155, "identity": 1.0, "score": 244.0, "evalue": 3.93e-79, "gene_start": 1, "gene_end": 146, "reference_start": 157, "reference_end": 302, "reference_sequence": "MSEQKQQAQPAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQWNPKMKQYIWGKRMGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFIWVGTKKQAKKAIESAAARTNSVYVSERWLGGTLTNSQTIFRSVRELERLEELQKSEYQGYTKKEGLLMDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE" }, "paralog": false, "identity": 0.991869918699187, "score": 433.0, "evalue": 9.52e-156, "description": "Internal stop codon at 706158, 706239. Nonsense mutation around 706158, 706239.", "psc": { "uniref90_id": "UniRef90_D1J8T8", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_D5E5E7", "cog_id": "COG0052", "cog_category": "J", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8T8", "UniRef:UniRef50_D5E5E7", "COG:COG0052", "COG:J", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5E7" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "LMJBGEANGM_1213", "locus": "GCA013368015_01213" }, { "type": "cds", "sequence": "contig_1", "start": 706820, "stop": 706936, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTAGATTTAAAAAAAATTAAAGAATTAAGAGAAAGAACAAATTCAGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAATAACGATATTGAAAAAGCAATTGAATGA", "aa": "MSVDLKKIKELRERTNSGFLDVKNALEATNNDIEKAIE", "aa_hexdigest": "70bdda38816f371176ed85c51c179563", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4333.8732, "isoelectric_point": 5.289686775207519 }, "id": "LMJBGEANGM_1214", "locus": "GCA013368015_01214" }, { "type": "cds", "sequence": "contig_1", "start": 706937, "stop": 707701, "strand": "+", "frame": 2, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_Q98Q37", "UniRef:UniRef90_D1J8T9" ], "nt": "TTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCCGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTGATTTTTGAATTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGAAGATTTATTAGAACAAAAAATTAACGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGTGAAAAGATTCATTTAAGAAGAGCATTAAAGTACGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAACAGAATTGCTACTATTTTAATCGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCCCTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAAGAAATTTATGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAGCAGAAAATAAATTACAATTAATAGATGCAACAAGATTTGAAGTTGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAA", "aa": "MQEKGIVKAAKKAGRIAAEGIVRAVIKDNIAVIFELNSETDFVAKNQLFLDFAKLLEEELPKVNFSNIEDLLEQKINGKTIEEYTTDLTAKIGEKIHLRRALKYEAKEDEVVAGYTHANNRIATILIAKGHNQEALRHVAMHIAALNPAHLFETCLDEEELKEIYERINNDPKLASKPEKIQVSMKSGMLRKEFNERGVLMYQPFVMEESKTVQQYLAENKLQLIDATRFEVGEGIEKKTVDFATEVAEQMSLK", "aa_hexdigest": "a19d50f5f5de7c5f806688862feb0ccb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8T9", "query_cov": 1.0, "subject_cov": 0.8668941979522184, "identity": 0.992, "score": 475.0, "evalue": 1.71e-168, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_Q98Q37", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q37" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "LMJBGEANGM_1215", "locus": "GCA013368015_01215" }, { "type": "cds", "sequence": "contig_1", "start": 707733, "stop": 708560, "strand": "-", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "RefSeq:WP_175936891.1", "SO:0001217", "UniParc:UPI00158F1C60", "UniRef:UniRef100_UPI00158F1C60", "UniRef:UniRef50_D1J8U0", "UniRef:UniRef90_D1J8U0" ], "nt": "ATGGAGAATAAATCTTTTAGTCAACTAGTAAAAGAAGAAATTATGTCTTTTCAGTTAAAAAAACAAGAAAAGTTTTTTTTGATGGCTGGCATAATTTATTGTTGCAGTGTAAAAAACAATGAAGCTAAATTAGTAATAAATAATATTAAGATTAGGACATATATATTAAATCTTTGCAATGAACTTAAAATTTCTTATACATATACTAAAAAAAATGAAATTTCAATAAATACAAATTTCTTAACTAACGTAAGTGTTAAATATCAAGGAAAATTCTTTGCTGGTTTATTCCTTTCTTCGGGTTCAATAATAAATGGAAGAACAAATCATTTAGAGTTAAAATTTAATGATTTAATAAATGCAGAAAATGCTTTATCAATTTTAAATTCTCACGAATTAGAATTTAAAAAAATAATTAGAAAACAAAAAATAATAATTTATTTAAAAAAAATTGAAAATATTTGTGATTTTTTAAAAGCTATTGAAACCATTGAATCTTATTATTTATTCGAAGATGGCAAAATTCAAAGAGATTTTTATAATTCGGCAAATAGAATAACAAATCTCGACGTATATAATCAAGAACGTATTGCAAAAGCAAATTTAGAATTTATTAAAAATTATCAATTTATAAAAGAAAACAATCTCTTGGATGAGTTTAGCGTTGAAGAACTTACACTATTTGAGTTAAAATTAAATAGTTTAGACAGTTCCTTGAATGAACTTAGTTTATTATTAGAAACAAAAAACATTAAAAAGTCTCGTTCTGCATTGAATCATTTCTTAATTAAACTAAAAAAAATAACTAAAAAGTATCAAAAAAAATAG", "aa": "MENKSFSQLVKEEIMSFQLKKQEKFFLMAGIIYCCSVKNNEAKLVINNIKIRTYILNLCNELKISYTYTKKNEISINTNFLTNVSVKYQGKFFAGLFLSSGSIINGRTNHLELKFNDLINAENALSILNSHELEFKKIIRKQKIIIYLKKIENICDFLKAIETIESYYLFEDGKIQRDFYNSANRITNLDVYNQERIAKANLEFIKNYQFIKENNLLDEFSVEELTLFELKLNSLDSSLNELSLLLETKNIKKSRSALNHFLIKLKKITKKYQKK", "aa_hexdigest": "e0bdbafaad59485f43d53fa1dcec78b9", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI00158F1C60", "ncbi_nrp_id": "WP_175936891.1", "uniref100_id": "UniRef100_UPI00158F1C60", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158F1C60", "RefSeq:WP_175936891.1", "UniRef:UniRef100_UPI00158F1C60" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158F1C60", "uniref90_id": "UniRef90_D1J8U0" }, "psc": { "uniref90_id": "UniRef90_D1J8U0", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_D1J8U0", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U0" ], "product": "DNA-binding protein WhiA" }, "genes": [ "whiA" ], "id": "LMJBGEANGM_1216", "locus": "GCA013368015_01216" }, { "type": "cds", "sequence": "contig_1", "start": 708609, "stop": 709136, "strand": "-", "frame": 1, "gene": "pncA", "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "COG:COG1335", "COG:HR", "RefSeq:WP_073673999.1", "SO:0001217", "UniParc:UPI000939710B", "UniRef:UniRef100_UPI000939710B", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_D1J8U1" ], "nt": "ATGAAAGCAATTTTTGTTGTTGATATGGTTAATGGTTTTTGCAAAGAAGGCGCACTTTATAGTAACAATATTGAGTCAATAATTGTTCCTATAAAAAATTTTTTAGAAACTCAATATAAAGACAATGATATATATTTTTTAAATGATGCTCATAGTAGCGATGATATTGAAATGCAATCATACCCAATACATTGTTTAAAAAATAGTAAAGAAAGCCAAGTAGTAGATGAATTAAAAAAATATGCTAAAAATATAATTGAAAAGAATTCTACTAACTCCTTTTTTGCATTAAAAAAGGAAATTTTAAGCAAGTATGATTCCTTTGAAATTATTGGTTGTTGTTCAGATATTTGCATTTTACAATTTGCAATAACTCTAAAAACTTATTTCAATCATTTAAAGCAAAATAAAGAAATTATTGTCTTTAAAAATTTAATTGCAACATTTAATATTTCAAATCATAATTCTCAAGAATATCACGATTTTGCTTTAAATTTAATGGCTAATGCAGGCATAAAAATTAAATAA", "aa": "MKAIFVVDMVNGFCKEGALYSNNIESIIVPIKNFLETQYKDNDIYFLNDAHSSDDIEMQSYPIHCLKNSKESQVVDELKKYAKNIIEKNSTNSFFALKKEILSKYDSFEIIGCCSDICILQFAITLKTYFNHLKQNKEIIVFKNLIATFNISNHNSQEYHDFALNLMANAGIKIK", "aa_hexdigest": "574085cbec88a469dc31ae6d13516613", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000939710B", "ncbi_nrp_id": "WP_073673999.1", "uniref100_id": "UniRef100_UPI000939710B", "db_xrefs": [ "SO:0001217", "UniParc:UPI000939710B", "RefSeq:WP_073673999.1", "UniRef:UniRef100_UPI000939710B" ] }, "ips": { "uniref100_id": "UniRef100_UPI000939710B", "uniref90_id": "UniRef90_D1J8U1" }, "psc": { "uniref90_id": "UniRef90_D1J8U1", "gene": "PncA", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748", "cog_id": "COG1335", "cog_category": "HR" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [ "pncA" ], "id": "LMJBGEANGM_1217", "locus": "GCA013368015_01217" }, { "type": "cds", "sequence": "contig_1", "start": 709308, "stop": 709460, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGA", "aa": "MAEEQLVVTSSPKKKIGFFSAILIVMGSAIGVGVFLRSKNVLQNSANNVV", "aa_hexdigest": "02596721837798f5e42380334bb00f3d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5293.2089000000005, "isoelectric_point": 9.996161460876465 }, "id": "LMJBGEANGM_1218", "locus": "GCA013368015_01218" }, { "type": "cds", "sequence": "contig_1", "start": 710106, "stop": 710261, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACACCCAGAAAAAACCCCTGCTGCTATTGTTATTGGTTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAACAACGGTTCATTCTACGGATTTGGAGATGAATTAAACGCTGTTAAAGCAGGTTGA", "aa": "MKHPEKTPAAIVIGLASMTVLYMLIAVTMTLGGNNGSFYGFGDELNAVKAG", "aa_hexdigest": "9b2780453f473bbd2db75d02ed91c875", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5301.184600000001, "isoelectric_point": 6.514055442810059 }, "id": "LMJBGEANGM_1219", "locus": "GCA013368015_01219" }, { "type": "cds", "sequence": "contig_1", "start": 710463, "stop": 710576, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCTTCACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGA", "aa": "MVIFTAIGAYAYFGGGYSTDYGYQIDNLLTFADLMAN", "aa_hexdigest": "80ebdad5c03bf7b06982cc073cecfbe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4048.5029000000013, "isoelectric_point": 4.0500284194946286 }, "id": "LMJBGEANGM_1220", "locus": "GCA013368015_01220" }, { "type": "cds", "sequence": "contig_1", "start": 710718, "stop": 710963, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCTCAATACGTTGCAGATAAAACACAAGACAACAGAAACGGCTTAGTAGGAAACATTGCTACATCAGTTCTATTTATAATATATGCAGCAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAACATTTGTGCACACGAAGTTCCAGAATGA", "aa": "MFFLAIDPFISIGLSAAQYVADKTQDNRNGLVGNIATSVLFIIYAAVSFLGAPVERQIAKSNKIKFDRVLSGNICAHEVPE", "aa_hexdigest": "2ded1a260e023ea3f24d5555973d742d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8786.054899999997, "isoelectric_point": 6.546226310729981 }, "id": "LMJBGEANGM_1221", "locus": "GCA013368015_01221" }, { "type": "cds", "sequence": "contig_1", "start": 711164, "stop": 711430, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAGATGAAACATTGATTAACACAAATTTAAAAACCGGCCTAAGCTCACAAGAGGCCACCTTAAGACTTAACAAGTTTGGAAAAAATGAGTTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAGCAATTTAAAGATATATTGGTCATTATATTATTATTTGCGGCAATAATATCTTATGCTCTTGCAATTGTTTCAGGAACTCAAAATAATTGA", "aa": "MKNSKEQINNIPYVLDETLINTNLKTGLSSQEATLRLNKFGKNELHKAKKINPFLIYLEQFKDILVIILLFAAIISYALAIVSGTQNN", "aa_hexdigest": "852159568dbcf72484506575f9497b4f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 56, "aa_cov": 0.6363636363636364, "hmm_cov": 1.0, "evalue": 8.383554791872906e-21, "score": 69.02449035644531, "start": 21, "stop": 76 } ], "seq_stats": { "molecular_weight": 9949.52249999999, "isoelectric_point": 9.30196590423584 }, "id": "LMJBGEANGM_1222", "locus": "GCA013368015_01222" }, { "type": "cds", "sequence": "contig_1", "start": 711560, "stop": 712069, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAAATTAAGCCCCTTACAAGCTAAAGTCGTCCGTGATGGTAATTTAATAACATTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGTTATTTGTTGTTTAGTTCAAATTTAACTGTTATCGAGAGTGCATTAACAGGCGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAGAGAAATTTAACATTAGCAATGGCAGATAGACAATTTATGGTTTATTCTTCAACTGTAGTTGCTACAGGAACTGCTTATTTTGTTGTTACATCAGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAATTAGTTAATCAACAAAAAGAATCAATGACTCCTCTACAAATTAAAATTAATAAATTAGGAAAAATATTTGGATATTCAGGTCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCGGTATCAAGAAATTCTTTTGCTGATACAATGTTTTGA", "aa": "MSKLSPLQAKVVRDGNLITLASSDITVGDIVLIESGDVVPSDGYLLFSSNLTVIESALTGESEPATKDYQAKRNLTLAMADRQFMVYSSTVVATGTAYFVVTSVGENNEIGKISKLVNQQKESMTPLQIKINKLGKIFGYSGLALFLASLVTQIVFHAVSRNSFADTMF", "aa_hexdigest": "dc8671917c66c2e4a8f271826dce9f44", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18220.733799999995, "isoelectric_point": 5.791972923278809 }, "id": "LMJBGEANGM_1223", "locus": "GCA013368015_01223" }, { "type": "cds", "sequence": "contig_1", "start": 712169, "stop": 712579, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGAAAGAATGCGATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGCAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAACAAGATGACAATTGTAGATGCTTTTGATGGAAGTCAACTACTATCAAATAATAGTGAAATTAAAAACTTTTTAAATATTATAGAATTAGGTTCGTTATGTTGCGATGCAAACTTGATTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTTTCTAATTTTAAATATAAAAACAACCTCGAAATAGAATATAAGCGTTTGCATTTGTTCCCGTTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACGACAACAATTATTGA", "aa": "MARKNAIVKNLMAVESLGNCAVICSDKTGTLTQNKMTIVDAFDGSQLLSNNSEIKNFLNIIELGSLCCDANLIRNEKDEYVVVGDPTEIAFLYELEKFSNFKYKNNLEIEYKRLHLFPFDSDRKMKSVINQNDNNY", "aa_hexdigest": "91ee42de69a8a2e8e034998a82d7be95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15527.539399999983, "isoelectric_point": 5.193060493469236 }, "id": "LMJBGEANGM_1224", "locus": "GCA013368015_01224" }, { "type": "cds", "sequence": "contig_1", "start": 712589, "stop": 713080, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGGGCGCTCCTGAAATAATAATTGAAAAATGTAATTTTGATTCACCTTCATTAAAAGAAAAATATTTGCAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTTTTAGCAATAGCAAGAAAATCTTTAAAAAAGGAAACAATTGAGAAATTCAATGAATCATCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATCGATCCTCCAAGAGAAAGTGCAAAAGATGCTATTACATTATGCAAACAAGCAGGAATAAAACCTGTTATGATTACTGGTGATAATTTAAATACAGCAAAAGCAATAGCAACAAATCTTGGTATATATAGTGATCAAGATCGCGCTATAACAGGAACTGAACTTTCAAACATTTCTGATGAAGAATTAGTTCAAAATATTGAAAAATACAGTGTTTATGCCAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCTTGA", "aa": "MGAPEIIIEKCNFDSPSLKEKYLQINNQWANNAYRVLAIARKSLKKETIEKFNESSISKLQEHVENDLEIVGLVAMIDPPRESAKDAITLCKQAGIKPVMITGDNLNTAKAIATNLGIYSDQDRAITGTELSNISDEELVQNIEKYSVYARVKPEDKLRIVNA", "aa_hexdigest": "d8e8a92940c2d3589194f1b47045d34b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 137, "aa_cov": 0.8220858895705522, "hmm_cov": 0.8686131386861314, "evalue": 3.804051509627495e-12, "score": 42.520755767822266, "start": 28, "stop": 161 } ], "seq_stats": { "molecular_weight": 18192.565899999994, "isoelectric_point": 5.331520271301269 }, "id": "LMJBGEANGM_1225", "locus": "GCA013368015_01225" }, { "type": "cds", "sequence": "contig_1", "start": 713105, "stop": 713383, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTGGCGATGGTGTTAATGATGCTCCGGCATTGAAAGCTTCAGATATAGGCTGCGCTATGGGTATAACAGGAACCGAAGCTTCTAAACAAGCTGCAAATGTTATATTGGCAGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAATGGAAGATCAATCTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCAATTGCAGAGATCATTTTATTATTTATAGGTTTATTTTTATTTAAAGCTATTTTTTCAAGTGCTGAATGA", "aa": "MTGDGVNDAPALKASDIGCAMGITGTEASKQAANVILADDNFATIVSAVANGRSIYQKIKNVIQSLLITSIAEIILLFIGLFLFKAIFSSAE", "aa_hexdigest": "d5e62797fa58ea977c0fde073636f9ca", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9562.986599999993, "isoelectric_point": 4.842193412780761 }, "id": "LMJBGEANGM_1226", "locus": "GCA013368015_01226" }, { "type": "cds", "sequence": "contig_1", "start": 713819, "stop": 714058, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGCTACACTGAAATTCATTCTAAGCCCGAACTTTTAGCACTAGGATTAGCATTGCCATTAGTAATTATTCCAATATATTCAATTCATAAATTAATCATATATTTAATTGAAAAAAACAAAATTAAAGAAAATAAAATTATTGAATTTAAAATGATATTACCTCCAAGGCAAGTCAAAAGAAATCAAAAAAGACATTAA", "aa": "MIVVFVKNINLVFGCYTEIHSKPELLALGLALPLVIIPIYSIHKLIIYLIEKNKIKENKIIEFKMILPPRQVKRNQKRH", "aa_hexdigest": "295a26bad85e423ffa7a1998dec52df2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9276.29189999999, "isoelectric_point": 9.943490791320801 }, "id": "LMJBGEANGM_1227", "locus": "GCA013368015_01227" }, { "type": "cds", "sequence": "contig_1", "start": 714139, "stop": 714435, "strand": "-", "frame": 3, "gene": null, "product": "DUF4190 domain-containing protein", "db_xrefs": [ "RefSeq:WP_241863100.1", "SO:0001217", "UniParc:UPI001F51A3F5", "UniRef:UniRef100_UPI001F51A3F5", "UniRef:UniRef50_D1J8U5", "UniRef:UniRef90_A0A6A8PZU7" ], "nt": "GTGGAAAAAGATGCATATGTAACAACACAAATGAATACAATGCAACCATCTCAACCTTATTTGCAACCTCAACAACAAATCCAAAATTCAGCAACTAATGAATTTGCTATTGTTGGATTTATATTGTCATTTTTCTTTTCGATATTAGGTCTTATTTTTAGCTGTATTGGACTTAGCAAGTCGAAATCAATGAATGATTCAGGAAGAACTTTGTCAATAGCAGAAATTATTATTTCTTGCTTAACTATGTTTTTTGCCTTTGTTATATTCGTTACTGTAATGAGCGTAAGCCTTTAA", "aa": "MEKDAYVTTQMNTMQPSQPYLQPQQQIQNSATNEFAIVGFILSFFFSILGLIFSCIGLSKSKSMNDSGRTLSIAEIIISCLTMFFAFVIFVTVMSVSL", "aa_hexdigest": "df0fb3be6a3628e6b331a20ca4304e9a", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI001F51A3F5", "ncbi_nrp_id": "WP_241863100.1", "uniref100_id": "UniRef100_UPI001F51A3F5", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F51A3F5", "RefSeq:WP_241863100.1", "UniRef:UniRef100_UPI001F51A3F5" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F51A3F5", "uniref90_id": "UniRef90_A0A6A8PZU7" }, "psc": { "uniref90_id": "UniRef90_A0A6A8PZU7", "product": "DUF4190 domain-containing protein", "uniref50_id": "UniRef50_D1J8U5" }, "pscc": { "uniref50_id": "UniRef50_D1J8U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U5" ], "product": "Zinc-ribbon domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_1228", "locus": "GCA013368015_01228" }, { "type": "cds", "sequence": "contig_1", "start": 714623, "stop": 714712, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTAAGGAAATGCACTTTATGCAACTTGACGGTGAAACTTTTGATATAGAAAATAAAATATATATAAACAAAAAAAATAATTAA", "aa": "MECKEMHFMQLDGETFDIENKIYINKKNN", "aa_hexdigest": "d6cca50a3343cd1146ea7eddf78105b4", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3564.0515000000014, "isoelectric_point": 5.00463924407959 }, "id": "LMJBGEANGM_1229", "locus": "GCA013368015_01229" }, { "type": "cds", "sequence": "contig_1", "start": 714914, "stop": 715504, "strand": "-", "frame": 2, "gene": "lCB5", "product": "Transcriptional regulator", "db_xrefs": [ "COG:COG1597", "COG:IR", "SO:0001217", "UniRef:UniRef50_D1J8U6", "UniRef:UniRef90_D1J8U6" ], "nt": "ATGCTATATATATTTTATAATTCTCTATCAAAGAGTGGAAAATGTCAAAGAAAGATGTATGACATAGTTTCTGACGCCATTAAAGTATTTTTACAACCTGAGGTAAAAATATTTGACATTATAAAAATTAAAGACAGTCAAAATCTAGTTTCTCAAATGGATATAGAAAAAGATATTGTTTTATTAATTGGCGGTGATGGAACATTAACCAATATCACTAATAAAATTCACAATATAAAACCTTTACCCAAAATTTATGCATATAAAGCAGGAACTGGCAATGATTTTCTAAGGTCTTTAAAAATAAAACAAGATGTTCAAATAATACATAAAAAATATTATTTGATATCTCCTTACATTACCAACTTACCAACAATTAAATTTAAAAACGACCAAGATGAAAGATCTTTTTTAAATGGCATTGGTTTTGGTATTGATGCAGTTATTGCAAAAAAAGTTAATGATGAAAAAATGCTTAAAAATAAATCGTCATTTGCTAAAGTAACAATAAATGCATTTAAGACATTCAAAACAATTCCTTTAATAAAAATTAATGTAGATGGAAAAGAATTAGAATTTAAAAATGTTTGA", "aa": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYITNLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNV", "aa_hexdigest": "4d9360ec907b86c58c4ad92e3c18d8ba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "lCB5" ], "truncated": "5-prime", "pseudogene": { "start": 714611, "stop": 715504, "observations": { "mutation": [ 714625, 714793, 714805, 714916 ], "stop": [ 714625, 714793, 714805, 714916 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U6", "query_cov": 1.0, "subject_cov": 0.6068111455108359, "identity": 0.995, "score": 383.0, "evalue": 1.17e-132, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYIANLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNVWLASIMNNCYFGGGMKLAPNCNNTDKLNLIIINNVSKWKLAWLFFSVYSGKHLRYKKYVHEIQGYKFSMECKEMHFMQLDGETFDIENKIYINKKKNQLNKKRPNKNWDVFLLLYCVIFIYKINLKK" }, "paralog": false, "identity": 0.9797979797979798, "score": 577.0, "evalue": 1.2e-211, "description": "Internal stop codon at 714625, 714793, 714805, 714916. Nonsense mutation around 714625, 714793, 714805, 714916.", "psc": { "uniref90_id": "UniRef90_D1J8U6", "gene": "LCB5", "product": "Transcriptional regulator", "uniref50_id": "UniRef50_D1J8U6", "cog_id": "COG1597", "cog_category": "IR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U6", "UniRef:UniRef50_D1J8U6", "COG:COG1597", "COG:IR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U6" ], "product": "Transcriptional regulator" } }, "id": "LMJBGEANGM_1230", "locus": "GCA013368015_01230" }, { "type": "cds", "sequence": "contig_1", "start": 715508, "stop": 716254, "strand": "-", "frame": 2, "gene": "argE", "product": "Xaa-His dipeptidase", "db_xrefs": [ "COG:COG0624", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZY24", "UniRef:UniRef90_D1J8U7" ], "nt": "ATGATCTATGTAACAGAAATTCATTTTCCAAAGGTTCCAGGAGTTGAATTAAATGGGGGCGACGTTGTTAACCAAATTCCTGATTCAACATCTTTAGTATTGAATGAAAAACCTAATATTATTAAGGGAATTGGTGGACACGGCTCAACACCTGAAAAAGGTGACAATGCAATAATTAAAGCCATTAATATAGCAAGTAAAGAAAATAAAGAATTTGCTAAATCTAAATTATTTAAATTTATTAAAGAAAATTTAAATAGCAAATCATTTAATTTAAGTAATATTTTTAAAAACTATAGTGATTTTTCGGGCGAATTATCTGCAAATTTAGGCATTATTAGAACCTTGAAAAATGAATATGTTTTAACTTTTGATTTTAGAGTCCCAGTTTCTCATAAAATTGATGATGTAACTAAAAGCTTAAATGATTATTTAAAAGATGAATTTGGCGATGATGTATTTTCAAAATTGCAAGGATTCAAAGAACCCAAGTATATTGAACAAGATTCTCCAATTGTAAGAATTCTTATGGATACATATAATGAAGCGATGAATTCAAATGAAAAACCTTTAGCAATTGGTGGCGGTACATTTGCAAGATTAATAAAAACTTGTGTCGCTTTTGGATCAACTAAATATATGCATTTAATGCATGGACCAAACGAATATTTTACATTTGATGAAATAAAATCATCACTTGAAATTTATATCAATGCATTAAATAGATTACAAGATTTGGAAAAATAA", "aa": "MIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGDDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEK", "aa_hexdigest": "5a54aa0d90e982c4bb7cec90702eb2d2", "start_type": "ATG", "rbs_motif": null, "genes": [ "argE" ], "truncated": "3-prime", "pseudogene": { "start": 715508, "stop": 716554, "observations": { "mutation": [ 716278, 716389, 716527 ], "stop": [ 716278, 716389, 716527 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U7", "query_cov": 1.0, "subject_cov": 0.5662100456621004, "identity": 0.992, "score": 485.0, "evalue": 3.62e-170, "gene_start": 1, "gene_end": 248, "reference_start": 191, "reference_end": 438, "reference_sequence": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQWRTSAFVPVITNESIIGRGSLDDKGPAIINLYAMKYIHDHGLLSNEWKIRIIFGISEETTMKSMKSYLADFKDPYISYTPDGEWPLIYAEKMIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGNDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEE" }, "paralog": false, "identity": 0.985632183908046, "score": 681.0, "evalue": 7.17e-250, "description": "Internal stop codon at 716278, 716389, 716527. Nonsense mutation around 716278, 716389, 716527.", "psc": { "uniref90_id": "UniRef90_D1J8U7", "gene": "ArgE", "product": "Xaa-His dipeptidase", "uniref50_id": "UniRef50_A0A448ZY24", "cog_id": "COG0624", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U7", "UniRef:UniRef50_A0A448ZY24", "COG:COG0624", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY24" ], "product": "Dipeptidase SA1572" } }, "id": "LMJBGEANGM_1231", "locus": "GCA013368015_01231" }, { "type": "cds", "sequence": "contig_1", "start": 716525, "stop": 716824, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTTATTACTTATAAGCAAAGTAAAGAAGACTTTGATGAAATGATTAAAAATATTGCAAATATTTGTGCAATTCCTTCGATTAGTGAAGAAGATTTTAGCAGTGAATTTCCTTTTGGAAAAGAAACTGACAATGCTTTAAATTATGCATTAAATCTTGCCAAAAGTTTTGGATTTAATATTTATAAAGATCCTAAAAATCGTTATGGGTTTGCTGAATTTGGCAATGGAGATAAGATTATTGGAATATTAGCCCACCTTGACGTTGTTCCTGCAGGAGATGAAAGCCAATGA", "aa": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQ", "aa_hexdigest": "6d5702e55358a66b66de891d7d2c573f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11122.363599999988, "isoelectric_point": 4.749943733215332 }, "id": "LMJBGEANGM_1232", "locus": "GCA013368015_01232" }, { "type": "cds", "sequence": "contig_1", "start": 717075, "stop": 717314, "strand": "-", "frame": 1, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8", "UniRef:UniRef90_UPI00068CCCF8" ], "nt": "TTGCAAGGTAATAAAATATCCAAAAATGGTACAAAAACTGGTTTGTCAGTTGAAGATGGTGGACCTGTTACTGAACCTATTAATGAATTTAAAGGTGATGTTGCTAGGGCATATTTATACTTTTGTTTAACTTATAACGATTTAAATATAACATCAAGTGATCAATCAACTCGTGTATTTAAAGAAATTAATAGAAAAAATACTATAACTCCTATATTTTTAAAAACATTTTTAGCTTGA", "aa": "MQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSSDQSTRVFKEINRKNTITPIFLKTFLA", "aa_hexdigest": "34ca119fdc2de85ce313f0b6e6a95bc0", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 716901, "stop": 717382, "observations": { "deletion": [ 717367 ], "stop": [ 717076 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00068CCCF8", "query_cov": 1.0, "subject_cov": 0.49375, "identity": 0.9620000000000001, "score": 156.0, "evalue": 2.59e-47, "gene_start": 1, "gene_end": 79, "reference_start": 24, "reference_end": 102, "reference_sequence": "MFDQPDKLVNAWHANYPYGTVKELQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSNNQSTRVFKEINRKNTITPIFLKTFLAWNNLDKVSKFDIDRNNGVYKHQGNRNPFIDYPKLIDVVYNNNNDYVFKNKGIAVGLNK" }, "paralog": false, "identity": 0.96875, "score": 320.0, "evalue": 2.62e-114, "description": "Internal stop codon at 717076. Frameshift due to deletion around 717367.", "psc": { "uniref90_id": "UniRef90_UPI00068CCCF8", "product": "endonuclease", "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00068CCCF8", "UniRef:UniRef50_UPI00068CCCF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8" ], "product": "endonuclease" } }, "id": "LMJBGEANGM_1233", "locus": "GCA013368015_01233" }, { "type": "cds", "sequence": "contig_1", "start": 717495, "stop": 718226, "strand": "-", "frame": 1, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAK4", "UniRef:UniRef90_UPI002410E520" ], "nt": "ATGAAAAAAACCAAAAAAATATTCCTAACTACGGGTGCTATTTTACCTTTGCTAATTTCAGCGCCATTAGTTGCTGCTGGATGTGTAAAAACAAAGAAATTTGAAGTTAAACCAAAAGGTGAAACCACAATAGAAAACATCATTAATAAACATAAAGAAATTAAAGATGATGAATTAAACAAAACTAAATCATTATTAGATCAATTTAAAAAATCAATTGAAGAAAGAACAAACCAAGCAGCATTAAACGAAGCAAAGAAGCAATTAAATTCAACTTTAAAACATGCGGCAAAAATAAATACAAAATCATTTAATTTTAACCAAAAATTTAATTTTAATAAAGAATATGTGAAAGGATTAGAAGCAATTAAAGGTGATGATTTGAATGCTATCAAAGAAATTACATTCAGATTAAATAGTTTTATAAATTTGGCGACATCACAAAAACACGGAACAACCCCTGAAAAGAAGCCTGAAGCTAAACCCTTAGATAATAAGATAATTTACGATAATTCAAATGACTTTTATAAATCCTTAGATGGCTTAAGCGGACAAAACCTAAGGGATAACTTATTTGCATTGCAAAAAGCTCATAGAAATCACTCAACAAAATATAAAGACTTATTTGATACATATAAAGATGCTTTTGTTGATAAATATTACGAAAAAGATGGATCTGTTTTAGATATCTATGAAGAAATTCCAGATAAAGAAGATGTTTTTACTAATTGA", "aa": "MKKTKKIFLTTGAILPLLISAPLVAAGCVKTKKFEVKPKGETTIENIINKHKEIKDDELNKTKSLLDQFKKSIEERTNQAALNEAKKQLNSTLKHAAKINTKSFNFNQKFNFNKEYVKGLEAIKGDDLNAIKEITFRLNSFINLATSQKHGTTPEKKPEAKPLDNKIIYDNSNDFYKSLDGLSGQNLRDNLFALQKAHRNHSTKYKDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTN", "aa_hexdigest": "bc7ba02bc29a628a0331687da8c1911f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 717192, "stop": 718226, "observations": { "mutation": [ 717377, 717422, 717497 ], "stop": [ 717377, 717422, 717497 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410E520", "query_cov": 1.0, "subject_cov": 0.5510204081632653, "identity": 0.992, "score": 443.0, "evalue": 8.56e-154, "gene_start": 1, "gene_end": 243, "reference_start": 1, "reference_end": 243, "reference_sequence": "MKKTKKIFLTMGAILPLLISAPLVAAGCVKTKKFEVKPKGETTIENIINKHKEIKDDELNKTKSLLDQFKKSIEERTNQAALNEAKKQLNSTLKHAAKINTKSFNFNQKFNFNKEYVKGLEAIKGDDLNAIKEITFRLNSFINLATSQKHGTTPEKKPEAKPLDNKIIYDNSNDFYKSLNGLSGQNLRDNLFALQKAHRNHSTKYKDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTNWHGKYRDQGKYEGDGMNREHLVPQSWFSKSVPMRTDAHHVWPTDKLVNAWHANYPYGTVKELQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSSDQSTRVFKENNGKNTITPIFLKTFLAWNNLDKVSKFDIDRNNGVYKHQGNRNPFIDYPELIDVVYNNNNDYVFKNKGIAVGLNK" }, "paralog": false, "identity": 0.9796511627906976, "score": 659.0, "evalue": 2.19e-241, "description": "Internal stop codon at 717377, 717422, 717497. Nonsense mutation around 717377, 717422, 717497.", "psc": { "uniref90_id": "UniRef90_UPI002410E520", "product": "endonuclease", "uniref50_id": "UniRef50_A0A454CAK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410E520", "UniRef:UniRef50_A0A454CAK4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CAK4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAK4" ], "product": "Ribonuclease" } }, "id": "LMJBGEANGM_1234", "locus": "GCA013368015_01234" }, { "type": "cds", "sequence": "contig_1", "start": 718384, "stop": 718497, "strand": "-", "frame": 3, "gene": "marR", "product": "MarR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035", "UniRef:UniRef50_O85035", "UniRef:UniRef90_O85035" ], "nt": "ATGAGAAAAGACGTTAGATTAGCTAACAAGGTAAAAGCAGCTAAAAGAGAACATAAATTAGCTTTAGAAAAAGCCAGAGCTCGCCGTAGACTTGCTAAACAACAAGCTGAATAA", "aa": "MRKDVRLANKVKAAKREHKLALEKARARRRLAKQQAE", "aa_hexdigest": "96ab6046e0d54d5a6a1bff54ed7fd2fb", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000B85C5", "uniref100_id": "UniRef100_O85035", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035" ] }, "ips": { "uniref100_id": "UniRef100_O85035", "uniref90_id": "UniRef90_O85035" }, "psc": { "uniref90_id": "UniRef90_O85035", "product": "MarR family transcriptional regulator", "uniref50_id": "UniRef50_O85035" }, "pscc": { "uniref50_id": "UniRef50_O85035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O85035" ], "product": "MarR family transcriptional regulator" }, "genes": [ "marR" ], "id": "LMJBGEANGM_1235", "locus": "GCA013368015_01235" }, { "type": "cds", "sequence": "contig_1", "start": 718534, "stop": 718791, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCGCTGAACATGCTTTGATTAAAAAATCCTATAATCAAGATATTCAACATTTAAATAATGAAGAACAAGAAGAACTATTTCAAATATCTACTGAAGATATTGAATTACCAAATGTTTCAAATAATGTTAATGACAACCAAAAAGATAAAAAAAATATAGCAACAAAAGAAAGCATTAGTCAGAAAATACAAGAAATTGAAAAAATAGATCTTGAAACAATAATGCAAAAAATTAAACAAATTAAGAAAAAATAG", "aa": "MTAEHALIKKSYNQDIQHLNNEEQEELFQISTEDIELPNVSNNVNDNQKDKKNIATKESISQKIQEIEKIDLETIMQKIKQIKKK", "aa_hexdigest": "f6cb0158332383a7042f4fca0d89bd41", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9979.142199999995, "isoelectric_point": 5.371705436706543 }, "id": "LMJBGEANGM_1236", "locus": "GCA013368015_01236" }, { "type": "cds", "sequence": "contig_1", "start": 718969, "stop": 719469, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATTAGGATATGCCAAAAAAGTTAATTTAAGAGATTTTGAAAATAAATCTTCTCTTAAAACAAAAACGTGCATGTCGTTTAAAGATGATAATGATGAATTAATAGATGCTCAAATTTCTAATGATGAAAAAATGCTATTTATTTTACTAAATAATGGTATGTATCATTTAGTTTCAGAAAACGAACTAAAAGTTGGAATTTCTTTGAAAGCAAGAGGCATTAGACTTCTTTTAAACTTATATAAACATCCTCAACTTCAAGTAAGTGGTTTTATAACAGTTTCAAAATACAACAATATAATTTATTTAACACAAGGTGGTTATATAAAATGTTGGGATACTAGCAAATTAGAATTGACCACACGCAATACTCCAAAAATGTTGTTTACGCCACTAAAAAATAATATTTTAGGTCTTCAATCACTTGCTGTTACATTGAGCAATTTAAAAATGTTATACACTGATAATAATGGTAATTTGGCAGAATATGATTGA", "aa": "MSKLGYAKKVNLRDFENKSSLKTKTCMSFKDDNDELIDAQISNDEKMLFILLNNGMYHLVSENELKVGISLKARGIRLLLNLYKHPQLQVSGFITVSKYNNIIYLTQGGYIKCWDTSKLELTTRNTPKMLFTPLKNNILGLQSLAVTLSNLKMLYTDNNGNLAEYD", "aa_hexdigest": "64c66a14de844e0d20ce8cd1b5c11297", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18935.7514, "isoelectric_point": 9.1135892868042 }, "id": "LMJBGEANGM_1237", "locus": "GCA013368015_01237" }, { "type": "cds", "sequence": "contig_1", "start": 719578, "stop": 719889, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAATAAAAAATCAATATTCAAAGCCAAGATTAACGGAAATTTCAGATCAAAAAATCGATAAAGAAATTGATCATAAATTATTGACAAAAAATGAAGATTTTTATTTATATATAACCAAAGATGGATATTATAAAAAAATAAGTTTAAAAGTTTATACTAGCAATGAATTAAACACATTCAAATTAAAAGAAGAAGATAATGTTTTCTATTTTGATAAAGTAAACTCGTTATCAAAGATATTATTCTTTACAAATTTAGGAAATTATTTTATTATTGATTGCCATTTGTTTAAAGATTGCAATTGA", "aa": "MLEIKNQYSKPRLTEISDQKIDKEIDHKLLTKNEDFYLYITKDGYYKKISLKVYTSNELNTFKLKEEDNVFYFDKVNSLSKILFFTNLGNYFIIDCHLFKDCN", "aa_hexdigest": "765b64ecf6ef4be0ec11af47519c0fcb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12428.095899999984, "isoelectric_point": 6.718846321105957 }, "id": "LMJBGEANGM_1238", "locus": "GCA013368015_01238" }, { "type": "cds", "sequence": "contig_1", "start": 719917, "stop": 720999, "strand": "-", "frame": 3, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "nt": "ATGCATGGTAATAAAGGTTCAATTGATGATGACCCTGCTGCTGCAATGCGTTACACTGAATCAAGACTTGAAAAAATTAGTGAACTGATGCTGAAAGATTTAGACAGAAAAGTTGTAAAAATGGTTCCAAACTTTGATGACTCTGAATACGAACCAATTGTTTTGCCGGCCTTATTTCCTAATTTATTAGTTAACGGTGCTAAAGGAATTGCCGCTGGTTTTGCTACAGAAATCCCACCACATAATCTAGGCGAAGTTATTGATGCAACAATTGCATTAATCAAAAATCCTACAATATCAATTGAAGAATTAAGTGAAATAGTTAAAGGCCCAGATTTCCCAACAGGAGCAATTATTAATGGTATAAATGAAATAAAAAAAGCTCTTTCAAGTGGGCAAGGTAGAATTACAATTTCTTCGAAATATCATTATGTTTATGATAAAAAAGATGAATCGAAAATTATTGGTATTGAAATAATTGAAATTCCTTTTGGGGTTGTTAAATCAAAATTAGTTGCCGACATTGATGCAATTGCAATAGATAAAAAAATTTCTGGTATTAAAGAAGTTTTGGACCAAACAGATAGAAACGGAATTTCAATATTTATTCAATTAGAAGACGGTGCAAATGCTGACGCAATAATTGCATACTTAATGAATAAAACCGAACTAAGTATCTCGTATAGTTATAACATGGTTGCAATTGACAATAACCGACCAGTAATTTTGAATCTCTATAGTGCTTTAATTGCTTATTTAAGTCATTTAAAAGAAGTTAATATAAATGGTATTAATTATGATTTAAAGAAGTTTAAATTGAGACTAGAAATAGTTGAAGGGTTTATTAAAGTAGCCGAAATTTCTGATGAAGTTATACATTTGATTAAAGAAAGCGATAACTCAAAAAAAGGTGTTATCCTTGCATTGATGAATAAATTTAAATTTAGCGAATTGCAAGCAACAGCGATTGCTGAATTAAGATTGTACAAGCTTTCAAGAATGGATCAAATTGAATTTCAAGAAGAAAAGAAAAACCTTGAAATTCAAATTGAAAATTGCAATAAATTATTAAATGATAAATGA", "aa": "MHGNKGSIDDDPAAAMRYTESRLEKISELMLKDLDRKVVKMVPNFDDSEYEPIVLPALFPNLLVNGAKGIAAGFATEIPPHNLGEVIDATIALIKNPTISIEELSEIVKGPDFPTGAIINGINEIKKALSSGQGRITISSKYHYVYDKKDESKIIGIEIIEIPFGVVKSKLVADIDAIAIDKKISGIKEVLDQTDRNGISIFIQLEDGANADAIIAYLMNKTELSISYSYNMVAIDNNRPVILNLYSALIAYLSHLKEVNINGINYDLKKFKLRLEIVEGFIKVAEISDEVIHLIKESDNSKKGVILALMNKFKFSELQATAIAELRLYKLSRMDQIEFQEEKKNLEIQIENCNKLLNDK", "aa_hexdigest": "8293e566644e10604a42d201d61b9cc8", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B04E97", "query_cov": 0.8611111111111112, "subject_cov": 1.0064935064935066, "identity": 0.51, "score": 300.0, "evalue": 2.17e-97, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_UPI0021B04E97" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B04E97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "LMJBGEANGM_1239", "locus": "GCA013368015_01239" }, { "type": "cds", "sequence": "contig_1", "start": 721153, "stop": 721335, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAAAAAAGATAGAAAAGAAGAAATACAAGAAGTTGCTGAAAACATTATTGAAAAAAATATGGCCGATATAATGTCTGATAGATTCGGGCGTTATTCAAAATACATTATTCAACAAAGAGCAATTCCTGATGCTCGTGATGGACTAAAACCTGTTCAACGTCGGATTTTATATTCAATGTGA", "aa": "MKKDRKEEIQEVAENIIEKNMADIMSDRFGRYSKYIIQQRAIPDARDGLKPVQRRILYSM", "aa_hexdigest": "73c5c120d7b60924c6b9961694df1bc4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 21, "aa_cov": 0.35, "hmm_cov": 1.0, "evalue": 1.1225635018719734e-10, "score": 36.42316436767578, "start": 40, "stop": 60 } ], "seq_stats": { "molecular_weight": 7187.269900000001, "isoelectric_point": 9.451403617858887 }, "id": "LMJBGEANGM_1240", "locus": "GCA013368015_01240" }, { "type": "cds", "sequence": "contig_1", "start": 721398, "stop": 721508, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCCGAAACTCGTTCAATAATTAAAGTTGATGTAGAAGATGAAATTTTAGTCGAAAGAAATGTTTCAACATTGATGAGTGACGACGTAATAGCAAGAAAACAATGA", "aa": "MNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQ", "aa_hexdigest": "099d210941b061bc58644c32aaae5669", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4144.682400000001, "isoelectric_point": 4.507070732116699 }, "id": "LMJBGEANGM_1241", "locus": "GCA013368015_01241" }, { "type": "cds", "sequence": "contig_1", "start": 721518, "stop": 721712, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTAGTTGAAAACGGTATGGTTTATATTGCTTTGCCACCTCTTTATAAGATTTCAATAAAATCAAAAAATACACAGCCCCTTTATGCTTGGGATGAAGATCAATTAAAAGAAATTACTTCAAAATATACCAACTATGAAATTCAACGCTACAAAGGGCTTGGCGAGATGAATGCTGATCAGCTTTGA", "aa": "MKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQL", "aa_hexdigest": "62171427d03e6284d882c45f788b6bc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7514.605900000001, "isoelectric_point": 7.907514762878418 }, "id": "LMJBGEANGM_1242", "locus": "GCA013368015_01242" }, { "type": "cds", "sequence": "contig_1", "start": 721719, "stop": 722675, "strand": "-", "frame": 1, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "COG:COG0187", "COG:L", "SO:0001217", "UniRef:UniRef50_Q59961", "UniRef:UniRef90_D1J8V0" ], "nt": "ATGCCAAATCTTACTATTGAATTTTATAATGAAAATACCGATGAACATGTAACATTTAAAACCAAAGATGGCATAAAAGAATATATAAATTTTGTTGGTGAAGGCAAAAAATTTATTAGTGAAATTTTTTATGCATCTGGTATGTGCAATTACGGCATTGAAATTGACTTGGCATTAGTATATACTGATGGATATAGTGAATCAATTTACAGTTTTGTAAATAATGTAAAGACTCGTGATGGTGGAACACATGAAACAGCACTTAAAACCGCATTAACTAAGACAATAAATGATTATGCTTCTAAAAATGGTTTAGCTAAAAAAATAACTTTTGAAGGATCGGATGTTAGAGAAGGAATAATTGCTGTTATTTCTTTAAAAGTCCCAGAAGAAATATTAGAATTTGTAGGTCAAACGAAAGATAAATTAGGTACGCCAGAGGCTAGAGAAGTTGTTGAAGATTTTGTAAGTCAAAAGTTTTATTTCTTTTTAAATGAAAACAAAATTGAAGCAGAAAAAATTATTTCTAAAATTAAAAAAGCATACGAAGCAAAAGTTGCAGCTAGAAACGCAAGAAACGAAGCACGTAAGATTAAAAATAAATTTGAGAATAGAAAGATTCTTTCAGGAAAATTAACTCCTGCTCAAAGTAAAAATCCTGCCATTAAAGAATTGTTTTTAGTTGAAGGTGATTCAGCTGGCGGTTCTGCTAAATTAGGAAGAAATAGAGTTACCCAGGCAATATTGCCACTTAGAGGTAAAGTTATCAACACCGATAAAGCAAAATTAAGCGATGTTTTAGCAAATGAAGAAATTGCAACAATAATTAATACAATCGGAGCGGGTATAGGCGAAGACTTCAATTTAAAAAATGCTCAATATCACAAAATTATTATTATGACAGATGCTGACACTGATGGTGCTCACATTCAAACATTACTATTAACATTCTTTTGA", "aa": "MPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFF", "aa_hexdigest": "6e326102553164995e849329219f0ca6", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E534FD0", "query_cov": 0.9842767295597484, "subject_cov": 0.8368983957219251, "identity": 0.534, "score": 318.0, "evalue": 4.62e-104, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A380FFC7" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 721416, "stop": 722975, "observations": { "mutation": [ 721520, 721721, 722756, 722861 ], "stop": [ 721520, 721721, 722756, 722861 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8V0", "query_cov": 1.0, "subject_cov": 0.49765258215962443, "identity": 1.0, "score": 610.0, "evalue": 3.59e-215, "gene_start": 1, "gene_end": 318, "reference_start": 196, "reference_end": 513, "reference_sequence": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTVWRDKKAYFTAFQQDTIIEKTHEISPTTKQGTKVQFWADFSIFKKSKYSPEIIKEKLREASFLMPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFFWRYMKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQLWETTMNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQWINNNVDFSSEDDFKINNK" }, "paralog": false, "identity": 0.9922928709055877, "score": 1002.0, "evalue": 0.0, "description": "Internal stop codon at 721520, 721721, 722756, 722861. Nonsense mutation around 721520, 721721, 722756, 722861.", "psc": { "uniref90_id": "UniRef90_D1J8V0", "gene": "GyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_Q59961", "cog_id": "COG0187", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8V0", "UniRef:UniRef50_Q59961", "COG:COG0187", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q59961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q59961" ], "product": "DNA topoisomerase 4 subunit B" } }, "id": "LMJBGEANGM_1243", "locus": "GCA013368015_01243" }, { "type": "cds", "sequence": "contig_1", "start": 722859, "stop": 723260, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGAGTTTAAATAATTACGAAGCTAGCGATTTAAAAGTTTTAAAAGGATTAGATGCTGTCAGAAAAAGACCGGGAATGTATATTGGGTCTACTGATTCAAGTGGGCTTCATCACTTAATTTGGGAAATTTTTGATAATGCTATTGATGAAGCGTTGAGTGGGTTTGCAAATAAAATAATTGTAACTTTAAAGAAGGATTCTTCTGTTATTGTTGAAGACAACGGTAGGGGAATCCCCGTCAATAAACACGAGTCTGGAAAAAGTGGAGTTGAGCTCGTCTTTACTGAGCTTCATGCTGGGGGCAAATTTGGCGATGGCGCTTATAAAACAAGTGGGGGATTACACGGAGTGGGTTCCTCAGTTGTCAATGCCTTAAGTTCTAAAGTTGAAGTTACGGTTTGA", "aa": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTV", "aa_hexdigest": "5ba540597d72b901175e8134d81c6abe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00037E1229", "query_cov": 1.0, "subject_cov": 0.8012048192771084, "identity": 0.677, "score": 187.0, "evalue": 1.9e-58, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A962E3V0" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 101, "aa_cov": 0.7368421052631579, "hmm_cov": 0.8514851485148515, "evalue": 1.5530339297961585e-17, "score": 59.5620231628418, "start": 32, "stop": 129 } ], "seq_stats": { "molecular_weight": 14056.700999999986, "isoelectric_point": 6.182343101501464 }, "id": "LMJBGEANGM_1244", "locus": "GCA013368015_01244" }, { "type": "cds", "sequence": "contig_1", "start": 723314, "stop": 723772, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTGTTCTTTAAGTGATTTATTAGTAGTTAAATACTACTTCAACGAACCGTATGATTCAATATTTAAATCTAATAATTTTATAAGAGATTTAAGATTAATAGGTAAGGCTAAGGATTATGTAATTGACTTTGCTTTTTGCGACAAAAATGTTATAAATTCAAATAAAAAAAATATATATAAATTAATTAAAAAAGAATTCAACAACGGCAAAGATTTAACATTGATTGTAGGCCACGATACCAACATTGCTACGACCTTGGCAATGTTAAACGTCAAAATGCCAGATCTTAAACAATTAGAAAAATATCCAATTGGGTCTAAATTAATTTTTGCAATTAATGATGACGATAGTTTTGATTTGTACATTGCTTTTTTTGACTTTAAAGACATTAGAAACTTTAATCTTTCAAATCCACAAATTATAAAAATCGCAAGCAAAATTTTTCTAAAATAA", "aa": "MACSLSDLLVVKYYFNEPYDSIFKSNNFIRDLRLIGKAKDYVIDFAFCDKNVINSNKKNIYKLIKKEFNNGKDLTLIVGHDTNIATTLAMLNVKMPDLKQLEKYPIGSKLIFAINDDDSFDLYIAFFDFKDIRNFNLSNPQIIKIASKIFLK", "aa_hexdigest": "628e1f7defe8ba9024473147f3168155", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17611.32629999999, "isoelectric_point": 8.939266777038576 }, "id": "LMJBGEANGM_1245", "locus": "GCA013368015_01245" }, { "type": "cds", "sequence": "contig_1", "start": 723965, "stop": 724141, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCAAAAAATTAGAGAATTTTTCAATATAACAAATGATATTGATGTTAAGGTGATGGCAAATTCAACGCAGAGAACTTTTCAAACTGCACAACTGCTTTCAATTGGTCTTTTTCCTGCTAAAAAAATCGACATTCAATGTCAAGACTATTCATTCAAAAATGAGAATGAATGA", "aa": "MAQKIREFFNITNDIDVKVMANSTQRTFQTAQLLSIGLFPAKKIDIQCQDYSFKNENE", "aa_hexdigest": "70039111a3ebf25bd3b5d10d37bcc4fd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6743.611099999998, "isoelectric_point": 6.154548835754394 }, "id": "LMJBGEANGM_1246", "locus": "GCA013368015_01246" }, { "type": "cds", "sequence": "contig_1", "start": 724416, "stop": 724673, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAACTAGAATTGCAATCAACGGTTTTGGTAGAATTGGTCGTCTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGATTTAACAGATGCTGCTACATTAGCTCACCTATTGAAATACGATACAGCTCATGGAAAATTGAATGAAAATATTTCACACACAGAAAATTCTATTATTGTAGATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGA", "aa": "MKKTRIAINGFGRIGRLIFREIFNDPEFEIVAINDLTDAATLAHLLKYDTAHGKLNENISHTENSIIVDGKEYRVYSEKDPLNLP", "aa_hexdigest": "8a2ede745bc0b33b3a92b3c754c39126", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9529411764705882, "subject_cov": 0.826530612244898, "identity": 0.654, "score": 113.0, "evalue": 5.77e-31, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 81, "aa_cov": 0.9529411764705882, "hmm_cov": 1.0, "evalue": 2.726400086912877e-32, "score": 106.64137268066406, "start": 5, "stop": 85 } ], "seq_stats": { "molecular_weight": 9685.897599999995, "isoelectric_point": 5.858588218688964 }, "id": "LMJBGEANGM_1247", "locus": "GCA013368015_01247" }, { "type": "cds", "sequence": "contig_1", "start": 724908, "stop": 725351, "strand": "+", "frame": 3, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "nt": "TTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTCCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACAGGTGCTGCAATAGCTATTGGAAAAGTTATCCCTTCATTGGATAAGAAACTAAATGGACTATCTTTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCCACTGTTCAAGAAATTAATGAAGCAATTAAAAAATCTGCTTCTGAATCATTAGAATATTCTACTGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGCGTGTTAGAAGTAGATGGCAAAAAACTATACAAAGTTTATGCATGA", "aa": "MEKNFGIEKGFMTTVHSYTADQRLQDAPHKDLRRARAAASNMVPTTTGAAIAIGKVIPSLDKKLNGLSLRVPTITGSFVDLTVELKKDATVQEINEAIKKSASESLEYSTEPIVSSDIIGAKAGSIFDSLLTSVLEVDGKKLYKVYA", "aa_hexdigest": "32d3a0bf25be8bdd953fd582a9364e55", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XW52", "query_cov": 0.9251700680272109, "subject_cov": 0.8553459119496856, "identity": 0.654, "score": 171.0, "evalue": 4.52e-52, "valid": false, "product": "Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain protein", "uniref50_id": "UniRef50_A0A3B0PH09" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PH09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "LMJBGEANGM_1248", "locus": "GCA013368015_01248" }, { "type": "cds", "sequence": "contig_1", "start": 725446, "stop": 725934, "strand": "+", "frame": 1, "gene": null, "product": "Septicolysin", "db_xrefs": [ "RefSeq:WP_036438585.1", "SO:0001217", "UniParc:UPI000512C9D7", "UniRef:UniRef100_A0A454CAF9", "UniRef:UniRef50_D1J8V3", "UniRef:UniRef90_D1J8V3" ], "nt": "ATGAAACTTTCAATAAAGGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTCGAAGATTTAAAAAAAGAAGAATCCAAGGAAAACTTCATTGTTTATTTAGAAAACAAAAGGCCAACTAACATTGAATATGATTATCAAGATTATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAATTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATACAAAAAATATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAAAAAATTTCTAGGGTTACTACAAACGATGGTCAAAACGAATATACGGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAAATTAAACGAAGAAATTTCTGAAATTCAAATTGCAATTGATAGTGCAAACATTAATACATTAGTTGAAGTTAAGTAA", "aa": "MKLSIKEIIKKIKLLELDLEDLKKEESKENFIVYLENKRPTNIEYDYQDYSNKIKNLYNEIFKLRTLVQVTNINTITSYKKYSISELIILLAQKSNQLERLQEMRDCKKISRVTTNDGQNEYTEYLFDPKMVAIELRKLNEEISEIQIAIDSANINTLVEVK", "aa_hexdigest": "7cbe3cf54989aa1c3bfaf47a086add7e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512C9D7", "ncbi_nrp_id": "WP_036438585.1", "uniref100_id": "UniRef100_A0A454CAF9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512C9D7", "RefSeq:WP_036438585.1", "UniRef:UniRef100_A0A454CAF9" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CAF9", "uniref90_id": "UniRef90_D1J8V3" }, "psc": { "uniref90_id": "UniRef90_D1J8V3", "product": "Septicolysin", "uniref50_id": "UniRef50_D1J8V3" }, "pscc": { "uniref50_id": "UniRef50_D1J8V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8V3" ], "product": "Septicolysin" }, "genes": [], "id": "LMJBGEANGM_1249", "locus": "GCA013368015_01249" }, { "type": "cds", "sequence": "contig_1", "start": 726182, "stop": 726364, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACAACAAAAGTATATATTGATTTTGAAGCAATTACCAACCCTTTTGCAAGATTAGTAAATTTGCCAAGTGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGCCAATATCAAATTAAAACATTTATTATTGATTTTAAAGAACATCATAATTTAAATTCAATTTGA", "aa": "MNTTKVYIDFEAITNPFARLVNLPSGTPYAYTLGLLNIHGQYQIKTFIIDFKEHHNLNSI", "aa_hexdigest": "8990b4dae1576a3af97b68c45f302653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6897.818599999997, "isoelectric_point": 6.8117780685424805 }, "id": "LMJBGEANGM_1250", "locus": "GCA013368015_01250" }, { "type": "cds", "sequence": "contig_1", "start": 726416, "stop": 726673, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAATTTAAAGGAAGTTGTATTTGTAGGTCACAATCCTGTTTTAGAAAATAAATGCTTAAAAAAATTATTTCCTGAAAATAATGTTGAACCTTTAATTTCCAATACAATTGTGTCATTGTCAAAATTAACAGGGAAAATTTTTAACATTCCATATTTTAATAAAATAAAAAAAATTATTGCTGATTCTGGTGATAGCGAACTTAATATGAGAATTAAAGATAGAAATGGTGTAATTGCTGCATTTGCTGGATATTGA", "aa": "MNLKEVVFVGHNPVLENKCLKKLFPENNVEPLISNTIVSLSKLTGKIFNIPYFNKIKKIIADSGDSELNMRIKDRNGVIAAFAGY", "aa_hexdigest": "eb894e435dfdfc2f3667dc164b07c035", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9522.076899999993, "isoelectric_point": 9.37423496246338 }, "id": "LMJBGEANGM_1251", "locus": "GCA013368015_01251" }, { "type": "cds", "sequence": "contig_1", "start": 726737, "stop": 726937, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCATGACGATGTCAATAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTTTACTAAAAGAACTTTTATACAAACGTGAACTAATAAAACAAATAAAAAATTTGGAACTTGACGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGA", "aa": "MNKNLLLKELKRYSHDDVNKMLYIVSHPDETETLLKELLYKRELIKQIKNLELDDNLTIKEIKEKI", "aa_hexdigest": "c5f9e23ce5844c46f86b1d1e4514c0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7996.301299999995, "isoelectric_point": 6.728338432312013 }, "id": "LMJBGEANGM_1252", "locus": "GCA013368015_01252" }, { "type": "cds", "sequence": "contig_1", "start": 727064, "stop": 727294, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGACAATTTCATGGTTAGCCATATTGATGGTGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTTTCTATTCAAGCAATCGGGGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGATTTTGATAAAGTAAAGCAGATAATGAATTTAAGAATTTGA", "aa": "MPIIIGEIIFNLNGLLDNFMVSHIDGGIDALAYANTYTGIIYTIFFSIQAIGAMFVGQYYGRKDFDKVKQIMNLRI", "aa_hexdigest": "d3969568ee1c60af6e60a2100fd3071a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8568.956099999996, "isoelectric_point": 5.485781288146971 }, "id": "LMJBGEANGM_1253", "locus": "GCA013368015_01253" }, { "type": "cds", "sequence": "contig_1", "start": 727469, "stop": 727795, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTAAATGAAACCGGACATTCAAAATACGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATACGGATTTCATAAACCTGCATATTATGCTGCTTTTGGTTCAATTATTGCTGCGGCAGTTTGCTTTACAAGTGATTCTTTATTTACATATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAATTGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCTGGAATGTTAACTTTACCTACAAGAATGATTATTTGA", "aa": "MLLNETGHSKYAFVSACLTMLTNASINSICLYGFHKPAYYAAFGSIIAAAVCFTSDSLFTYYKERKIFINVFKLFHITKPIAKQLLKRIPAMLIAIAGMLTLPTRMII", "aa_hexdigest": "dea4ca7d7358ced9b48335dafbb9f039", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12088.445999999982, "isoelectric_point": 9.676914024353028 }, "id": "LMJBGEANGM_1254", "locus": "GCA013368015_01254" }, { "type": "cds", "sequence": "contig_1", "start": 727850, "stop": 728353, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCGTCCGTCGCCTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCGTTTTTTGTTGCCACAAGATTAGGAGAGTCTGATTTTGAAGGTGCAGATAAGCATGCTCATTCATTGCGGGGTTTTCATGCTTTGGCTGGATGTTGCATGTCTATATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTACTACAACAAAAGGTGTTGGTATTGGTGTCTCTCAAAGATTTTATGATGTGCAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAATTCACTTGGCAATGATTTTACATTCACAAAAGGTCAAAATTTGGCTTATGACCAATGGGTGGCAAGCGCAGTACTGGCAACTTCAAAAGAATTCAAAGAAACATTTTTGTTATGTAGTGCAACCTTTATTTTCTTTAATCCTATATGA", "aa": "MGINAVTILGLVESLSSVASAITSVCASNVSFFVATRLGESDFEGADKHAHSLRGFHALAGCCMSILMIAVVFGIAYSPTTTKGVGIGVSQRFYDVQRKDYIIEILNKSDILKNSDLLNSLGNDFTFTKGQNLAYDQWVASAVLATSKEFKETFLLCSATFIFFNPI", "aa_hexdigest": "7f919548b8a1c7851bc8d5526c59b7ed", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18024.49699999999, "isoelectric_point": 5.85478000640869 }, "id": "LMJBGEANGM_1255", "locus": "GCA013368015_01255" }, { "type": "cds", "sequence": "contig_1", "start": 728725, "stop": 729030, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00561.26" ], "nt": "ATGTTATATGATTTAGTTTCACCATATAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGCAAGCCAATTTTGTTTGTCCATGGTGGTCCAGGTGGGGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGTGGACTTAGCATACCTAGTGCCGAAATCAAACAAAATACGACCCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGA", "aa": "MLYDLVSPYNSGHLKVSDIHSIYYEESGNPNGKPILFVHGGPGGGTKPSCRQFFDPEFYRIILFDQRGCGLSIPSAEIKQNTTQDLIEDIEKLRKFLNIDK", "aa_hexdigest": "049e0eec9caeeee4c69317414b102aa2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A1L4BUW4", "query_cov": 0.9702970297029703, "subject_cov": 0.9245283018867925, "identity": 0.633, "score": 138.0, "evalue": 1.23e-40, "valid": false, "product": "Proline iminopeptidase", "uniref50_id": "UniRef50_A0A1L4BUW4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00561.26", "name": "Abhydrolase_1", "length": 71, "aa_cov": 0.6831683168316832, "hmm_cov": 0.9859154929577465, "evalue": 2.0531520957489525e-10, "score": 36.224388122558594, "start": 33, "stop": 101 } ], "seq_stats": { "molecular_weight": 11419.813099999985, "isoelectric_point": 5.849380302429199 }, "id": "LMJBGEANGM_1256", "locus": "GCA013368015_01256" }, { "type": "cds", "sequence": "contig_1", "start": 729154, "stop": 729309, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCAAAAAGGCGCTTCTTATTATTTTCCTGCTGAATATGAAGAATATTCAAACTTTATTCCTGAAAATGAAAGAGGCGATTTAATTAACGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGA", "aa": "MYQKGASYYFPAEYEEYSNFIPENERGDLINAYYKYLNSSDQNVAEQAAYH", "aa_hexdigest": "e9321f42b69b295c7098dd81562c2c79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6056.417700000004, "isoelectric_point": 4.375886344909667 }, "id": "LMJBGEANGM_1257", "locus": "GCA013368015_01257" }, { "type": "cds", "sequence": "contig_1", "start": 729337, "stop": 729579, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTGCCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGCAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAAATATTTTTAAATGACGATAATTTTATTTTAAATAATGCAAGCAAGATTAAAGAAATCCCAACAATATTGATTCATGGTCAATTTGATATGGATTGTCGCCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAGTCAAAGCTTTGA", "aa": "MKKLPNLEEILSDKKSNLELARLENYYFVNKIFLNDDNFILNNASKIKEIPTILIHGQFDMDCRPVGAYLLHKALPKSKL", "aa_hexdigest": "8eff35bcba50ad8ab78d3385e415356d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9289.797099999992, "isoelectric_point": 8.734515190124512 }, "id": "LMJBGEANGM_1258", "locus": "GCA013368015_01258" }, { "type": "cds", "sequence": "contig_1", "start": 729756, "stop": 730301, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09371.15" ], "nt": "ATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCAGAAGGGGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTGTATGATGTTGAATTAAATAAAAGAAAAGAAGCCATATTGAATATATTGGCCGAAAATAAATTATTAAATGATGAACTAAAACAAAAAATCGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAAGCATCAGATGCTATTGCTCTTGGTTTAAGTCCGTTGGCCGATGAAATTTTAAATAATAATGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGA", "aa": "MNSINNVAKKLNLSENQVKNTLSLLSEGATVPFISRYRKNITGGLDEDQITKINDLYVYDVELNKRKEAILNILAENKLLNDELKQKIEAADTKQAVENIYEPFKIGKKTKASDAIALGLSPLADEILNNNDDNYNLYYAAKKYISDKLKTEDEVLQQTKFIIAQNISQDPSTREYVKNQL", "aa_hexdigest": "fda9b6997e32972ffd4ac1a74bff54fa", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09371.15", "name": "Tex_N", "length": 73, "aa_cov": 0.40331491712707185, "hmm_cov": 1.0, "evalue": 2.2282021282203764e-29, "score": 96.57521057128906, "start": 2, "stop": 74 } ], "seq_stats": { "molecular_weight": 20543.994799999997, "isoelectric_point": 5.389893913269043 }, "id": "LMJBGEANGM_1259", "locus": "GCA013368015_01259" }, { "type": "cds", "sequence": "contig_1", "start": 730665, "stop": 730814, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16921.10" ], "nt": "ATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCATAATCAAGAACAATGA", "aa": "MLLSPATKNKRILAIDPAYVNGCKIAAIDQNGNFLKEGIIYPHNHNQEQ", "aa_hexdigest": "738eccb456a93190e2c542b3ed3fb0da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16921.10", "name": "Tex_YqgF", "length": 38, "aa_cov": 0.7755102040816326, "hmm_cov": 1.0, "evalue": 6.942961398859069e-13, "score": 44.69309997558594, "start": 10, "stop": 47 } ], "seq_stats": { "molecular_weight": 5463.2110999999995, "isoelectric_point": 7.928402519226075 }, "id": "LMJBGEANGM_1260", "locus": "GCA013368015_01260" }, { "type": "cds", "sequence": "contig_1", "start": 730926, "stop": 731903, "strand": "+", "frame": 3, "gene": null, "product": "S1 RNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "nt": "TTGCAAAAAAGACTTGAAAAAAATCCTCATGAAAAAATCAAATTTGTAATTGTAAGTGAAGTTGGCGCTTCTGTTTATTCAGCTTCAAAAATTGCTCAAGAAGAATTTCCACAATTACATGTTGAATATAGATCAGCAATTCATATTGGTAGAAAGTTTCAAGATCCCTTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAAGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTTGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAATACCATAGCAAGCAATATTGTAAACTATAGAGAAGAAAACGGTTCATTTAAATCACGCGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACTATTGTCAATAAGCTAAATATAGATTTGAACAATATTGACAAAGAAAAATTATTATCCGTTGATAAAGAACAACTTGCAAAAGAATTAAACTCTGATAAATACAGCATTCAACTAATTATAGAATCATTGATTGCACCTGAAAAAGATATTAGAGATGATAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATCGATGATATAACTCAAGGTCAAATAATTAAGGGTCAAATTCAAAATGTTACCGATTTTGGCGCCTTTGCGTTTATAGGATTAAAACAAAACGTTTTGATCCATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAATATACAAATAATTTCCATTGATAAAGAACACAATAAAATTCAAGGAAAAATTATTTGA", "aa": "MQKRLEKNPHEKIKFVIVSEVGASVYSASKIAQEEFPQLHVEYRSAIHIGRKFQDPLNELIKIDPKSIGIGQYQHDVNQKELSKQLTEKLNKVVNLVGVDLNTATKVILSYISGLSNTIASNIVNYREENGSFKSREELKKVKGLGPKAYEQSVGFLRIHDSNNFYDKTNIHPESYKLADTIVNKLNIDLNNIDKEKLLSVDKEQLAKELNSDKYSIQLIIESLIAPEKDIRDDKPGFLVSDKVLTIDDITQGQIIKGQIQNVTDFGAFAFIGLKQNVLIHITNMKKKENQFIKHPLDVLNVGDNVNIQIISIDKEHNKIQGKII", "aa_hexdigest": "5cb27132aa2d5add4614cd6d4fb50fc7", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003593DDCE", "query_cov": 0.8615384615384616, "subject_cov": 0.9929078014184397, "identity": 0.7390000000000001, "score": 412.0, "evalue": 1.23e-142, "valid": false, "product": "helix-hairpin-helix domain-containing protein", "uniref50_id": "UniRef50_A0A502M9J9" }, "pscc": { "uniref50_id": "UniRef50_A0A502M9J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "product": "S1 RNA-binding domain-containing protein" }, "genes": [], "id": "LMJBGEANGM_1261", "locus": "GCA013368015_01261" }, { "type": "cds", "sequence": "contig_1", "start": 732130, "stop": 732957, "strand": "+", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAGCTTCAAGCAGGAATTGAAGACTTTGATTATGCAACTTTATCAGATGAAGAAGCGCTGACAGGAAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAAAAACGCTCCAACAAACAATTGTTTAAATGAAACATTGGACAATATCTTTAAAAATATTGAATCATCAGCAAAGGGCCAACAAAGTGAAAATGATTTTTCAGGTTTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGAGATGCTTATGAATATTTAATGTCAATGTATGCATCGAATGCTGGAAAATCAGGGGGTGAATATTTTACTCCTCAAGAAGTTTCAGAATTGCTTACTAAAATAGCGGTTTTCAATAAGAAAAAAGTCAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTACTCCTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGTCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATTGGATTTGATAAATTCAACATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATCGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQLQAGIEDFDYATLSDEEALTGRDDLINEKGFFILPSELFINVVKNAPTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "f6336111d18b09d380d467b8a250766d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.662, "score": 369.0, "evalue": 1.35e-125, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "LMJBGEANGM_1262", "locus": "GCA013368015_01262" }, { "type": "cds", "sequence": "contig_1", "start": 732976, "stop": 733578, "strand": "+", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "nt": "TTGTTAATAAATGACCAAAGATTTTCACCAGCTGGAATATTAGCTCCAAAAAGCAAAGCCGATTTTGCTTTCATACTTCATAGCCTTTCATGGCTTGCAACTGATGGAGTAGCTGCAATAGTTTGTTTTCCGGGCATTATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAATTTGTTTTTCGGAACAAGTATTTCAACATGCATAATGGTATTAAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAATAAATTAACTAGTCAAAATATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCAAAATTAGCTAGTGTTGAAGACGTTAAATCTAATTCATATAATTTATCAGTCAATAGTTACGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGCGAAATTGACAAAATAGTTGCCGAATTAGAGGAATAG", "aa": "MLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQRKDISYISKLASVEDVKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "8fb3fafc72858d50430b43bd49db477c", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI0025CE0BCD", "query_cov": 0.995, "subject_cov": 0.9170506912442397, "identity": 0.6629999999999999, "score": 267.0, "evalue": 2.29e-88, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A132HPU2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A132HPU2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A132HPU2" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "LMJBGEANGM_1263", "locus": "GCA013368015_01263" }, { "type": "cds", "sequence": "contig_1", "start": 733581, "stop": 733817, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAATAGAAAAGTTAATTAGGGAACTATGTCCTAATGGAGTTGAATATAAAAAATTATGTGACTCTTCAATAATATCAGTTGGTAGTAGAATAACTAAATCAATGATGAATGAAAGTGAAAAATATCCTGTTTATGGTGGTGGAACAATTCCAACAGGATATTATAATGATTTTAATAATGAACATTCAATAGCTATTTCAAGAGCAGGAAGTGCAGGATCAGTAAAATGA", "aa": "MNKIEKLIRELCPNGVEYKKLCDSSIISVGSRITKSMMNESEKYPVYGGGTIPTGYYNDFNNEHSIAISRAGSAGSVK", "aa_hexdigest": "27cbf52137f0d883e57571aae0cccaed", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8549.637799999997, "isoelectric_point": 8.59861583709717 }, "id": "LMJBGEANGM_1264", "locus": "GCA013368015_01264" }, { "type": "cds", "sequence": "contig_1", "start": 733818, "stop": 733982, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGCCAAAAATATTGGGCTACTGATGTATGTTTTGTTGTGTCTGAAAAATATGAAGTTGCAAATATTAAATTTTTATATCATTTTTTAAAATTAAGAGAAAATGAATTAAAAAAACACATTTATGGAGGAAATTTACCTAAATTAGATAAACAATACTTATGA", "aa": "MSQKYWATDVCFVVSEKYEVANIKFLYHFLKLRENELKKHIYGGNLPKLDKQYL", "aa_hexdigest": "5dccb30f9299e966d077b0056f1ac2f1", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6540.565699999999, "isoelectric_point": 9.099277305603028 }, "id": "LMJBGEANGM_1265", "locus": "GCA013368015_01265" }, { "type": "cds", "sequence": "contig_1", "start": 733986, "stop": 734246, "strand": "+", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E04BC5F", "UniRef:UniRef90_UPI0034D97483" ], "nt": "TTGCAAATTCCGCTCCCCCCACTTGAAATTCAAAATCAAATTGTTAATATTTTAGACAAGTTCGCCGAGTTGACCGCCGAGTTGACCGCCGAGTTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAGAATTATTAAATAAGATAATGAATAAACAACAATGTGGTAATAGTATAGTTGAATATAAAAAATTAGAAGAAGTAGTTGACTATTTACAACCTACTAAATGA", "aa": "MQIPLPPLEIQNQIVNILDKFAELTAELTAELTYRDKQYNYYRNKLLDFDNNKELLNKIMNKQQCGNSIVEYKKLEEVVDYLQPTK", "aa_hexdigest": "7403534f17ad47a67ebdf02cb79f0a93", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D97483", "query_cov": 0.9534883720930233, "subject_cov": 1.0, "identity": 0.902, "score": 127.0, "evalue": 8.65e-37, "valid": true, "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI000E04BC5F" }, "pscc": { "uniref50_id": "UniRef50_UPI000E04BC5F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E04BC5F" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "LMJBGEANGM_1266", "locus": "GCA013368015_01266" }, { "type": "cds", "sequence": "contig_1", "start": 734823, "stop": 734972, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTGTGAAATAAAGCGAGGAAAAGTTTATTCAAAGGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAGACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGA", "aa": "MCEIKRGKVYSKEFIKLNKGEYPVYSSQSLDDGILGKIDKYDFDGEYVT", "aa_hexdigest": "33f01b1b5230704a21430e10d432728a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 1.0, "hmm_cov": 0.9811320754716981, "evalue": 1.1588432219037254e-08, "score": 30.93366050720215, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5671.3654000000015, "isoelectric_point": 5.170609092712401 }, "id": "LMJBGEANGM_1267", "locus": "GCA013368015_01267" }, { "type": "cds", "sequence": "contig_1", "start": 735036, "stop": 735392, "strand": "+", "frame": 3, "gene": null, "product": "Type I restriction modification DNA specificity domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K2KEM7" ], "nt": "GTGTGTGGAATATTGAGTGTTCTAAATAAATCAAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGAAGACAAAAAAATTTGTAAACAAGGCAAGCGGAAATCCTAAACTCATGAGTAATATTATGGAAAATATTGAAATCCCCATCCCTCATATTTCAATTCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATACACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGA", "aa": "MCGILSVLNKSKLNVKYLSTCLSMKTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYLLLLNEL", "aa_hexdigest": "9647872c6b2423720bd2d2624da235e9", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000B075C54", "query_cov": 0.8220338983050848, "subject_cov": 1.0, "identity": 0.515, "score": 98.2, "evalue": 1.71e-24, "valid": false, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_K2KEM7", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_K2KEM7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_K2KEM7" ], "product": "Type I restriction modification DNA specificity domain protein" }, "genes": [], "id": "LMJBGEANGM_1268", "locus": "GCA013368015_01268" }, { "type": "cds", "sequence": "contig_1", "start": 735486, "stop": 735953, "strand": "+", "frame": 3, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A454CAF2", "UniRef:UniRef90_A0A454CAF2" ], "nt": "ATGAACAAAGGGGATTATTTAGCAATAACTTCAGGAAATAAATCATTTTATGTCTCTGATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGTGGGCAAGCAGACGTTAAATATAATCAAGGAAAAACAATGTATTCCGATCATGTGTTTGCGTTTAAAACAAATGAATGCAACACAAAATATCTTTACTTTTATTTATTGAATATTTCTAATTTTATTAACGAAAAATTATTTGTTGGTAGTGGATTAAAAAATTTAAATAAAAAAGAATTCTTGAATCTTAATATTGCAATTCCTCCTATTGAAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAAACTGGTCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAACATTACAGGGATAAATTATTGAACTTTAAAGAAAAGAATACTAAAATTTAA", "aa": "MNKGDYLAITSGNKSFYVSDFLVDGEYIFVNDGGQADVKYNQGKTMYSDHVFAFKTNECNTKYLYFYLLNISNFINEKLFVGSGLKNLNKKEFLNLNIAIPPIEIQNKIVEILDKLQAYTKDIQTGLPLEIDQRKKQYEHYRDKLLNFKEKNTKI", "aa_hexdigest": "bc26592f60119368824511b8924b14e1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAF2", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.987, "score": 305.0, "evalue": 3.75e-105, "valid": true, "product": "Restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A454CAF2", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF2" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "LMJBGEANGM_1269", "locus": "GCA013368015_01269" }, { "type": "cds", "sequence": "contig_1", "start": 735985, "stop": 736224, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction-modification system, restriction subunit R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "nt": "ATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACAGTTGTTTCTGAATATAAGCCTCTAGCGAATAAAGCAAAAGATTATCAAACAGAGAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAACCAAGGATATGAGTATTTGAACATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGTTAAATAAAATAAATTTTTCAGATAATGAATGA", "aa": "MEKYNIIVENENDTVVSEYKPLANKAKDYQTESELESQFISMLKNQGYEYLNIHNEEDLINNLRQQIELLNKINFSDNE", "aa_hexdigest": "cc1b22fe18b7ae4c95a225b4c13a441b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A0F5H0F8", "query_cov": 1.0379746835443038, "subject_cov": 1.0, "identity": 0.524, "score": 71.2, "evalue": 1.14e-14, "valid": false, "product": "Type I restriction-modification system, restriction subunit R", "uniref50_id": "UniRef50_A0A0F5H0F8" }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H0F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "product": "Type I restriction-modification system, restriction subunit R" }, "genes": [], "id": "LMJBGEANGM_1270", "locus": "GCA013368015_01270" }, { "type": "cds", "sequence": "contig_1", "start": 736447, "stop": 736710, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAATGGCATTCCAATGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTCTAAAAGAGGCTTTTAATCAAATCATTAGATATCAAAGAGATTCGTTTTGGGCTAGCAACGGCTTATATGAATATGTACAAGTGTTTGTTATTTCAAACGGGGCAAATACCAAATATTATTCAAATACAACAAGATTACAACATGTTAATAAAAAATCAGATATGATAGATTCAAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGA", "aa": "MVNGIPMVHIELKRRGVALKEAFNQIIRYQRDSFWASNGLYEYVQVFVISNGANTKYYSNTTRLQHVNKKSDMIDSKTSKSFEFTHF", "aa_hexdigest": "c5fc9c08793e399412bd90854a98a26c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10196.490599999994, "isoelectric_point": 9.695351982116701 }, "id": "LMJBGEANGM_1271", "locus": "GCA013368015_01271" }, { "type": "cds", "sequence": "contig_1", "start": 736825, "stop": 736941, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTAGTAATGAGACCTTATCAAATTGTTGCTGTTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCAGGTGGCTTTATTTGA", "aa": "MLLVMRPYQIVAVEKIINKINISHNYKKYGSIAAGGFI", "aa_hexdigest": "1c95e9d0dc4ab6e276ca285323d80814", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4294.1347000000005, "isoelectric_point": 9.873542594909669 }, "id": "LMJBGEANGM_1272", "locus": "GCA013368015_01272" }, { "type": "cds", "sequence": "contig_1", "start": 736966, "stop": 738381, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction enzyme endonuclease subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7GZJ3" ], "nt": "TTGACCTCTTTTAAAACTGCAAAATTAGCATCACAATTGCCTTATATAAATAAAGTAATTTTTGTTGTTGATAGAAGAGATTTAGACTATCAAACAATGAAGGAATATGATAAATTAGAAAAAGGCGCTGCAAATTCAAATCGTTCTACATTAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCTGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTGCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCAGAAGTGATTAAAACAACCGAACAAGCTTTCGGCCAAAAATTGCACACTTATACAATAATAAATGCAATAAAAGATAGAAACGTTTTGCCATTTAAAATTGAATATATTAAAACAATTGATGTTGATAAATCGCTAAACGATAAAGAGGATCAAAAAGTAAAAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTTGATCAAAAAACTAGGAGAAATTCCAATGTAATTTATAGCCATTCGATATTTGATAAATTAGATAAAACTATTAAAAGAAACGTGAGCGGTTTCAATTCTATTATGGCTGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAACCAACTTGCTCAAGATCCTAAAAACAATCTAAAAATTGCTTTAATATATTCATATGGGGTTAATACCGAGGAATCATATGGCGGTTTAATTGATGAAGAGGATAATGAAAATACATTGCAATTAAGTAAGCCAGATAGAGATTTTTTGGATGGTGCTATTAAAGATTATAATAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTAGACATGCTGATTGTAGTAAACATGTTTTTAACTGGTTTTGACGCCCCAACATTAAATACATTATGGGTTGATAAGAAACTAAGAGAACATGGTTTAATTCAAGCTTTTTCAAGAACAAATAGAATTCTAAATGATAAAAAGGTATATGGAAATATTGTATGTTTTAGAAATTTAGAAAAAGAAACTGACCAAGCAATTTCGATATTTGGCGATGGGGAAGCTAGTGGAATTGTTAAAATCAAAAATTTCGTTGACTATTATTATGGCTATAATGATAGTCAGCAAGTGCATCACAATGGCTATTGCGATTATATTCAAAAATTAAATAATGAATTTCCTTTAGATAATTGA", "aa": "MTSFKTAKLASQLPYINKVIFVVDRRDLDYQTMKEYDKLEKGAANSNRSTLILQRQLESNDPNKKIIITTIQKLSIFIKNNRNHPVFDKEVVLIFDECHRSQFGAMHTAVIKNFKKYYLFGFTGTPIFAANINKSSPEVIKTTEQAFGQKLHTYTIINAIKDRNVLPFKIEYIKTIDVDKSLNDKEDQKVKDIDRESVFLDQRRIKLVVEYILKHFDQKTRRNSNVIYSHSIFDKLDKTIKRNVSGFNSIMAVSSIEMAKKYYLEFKNQLAQDPKNNLKIALIYSYGVNTEESYGGLIDEEDNENTLQLSKPDRDFLDGAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTLWVDKKLREHGLIQAFSRTNRILNDKKVYGNIVCFRNLEKETDQAISIFGDGEASGIVKIKNFVDYYYGYNDSQQVHHNGYCDYIQKLNNEFPLDN", "aa_hexdigest": "a895285a335365eda77d622d82de9935", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0026DF2CF8", "query_cov": 1.048832271762208, "subject_cov": 0.903107861060329, "identity": 0.579, "score": 530.0, "evalue": 1.51e-182, "valid": false, "product": "UvrB domain 3-containing protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A7X7GZJ3", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A7X7GZJ3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X7GZJ3" ], "product": "Type I restriction enzyme endonuclease subunit" }, "genes": [], "id": "LMJBGEANGM_1273", "locus": "GCA013368015_01273" }, { "type": "cds", "sequence": "contig_1", "start": 738421, "stop": 739068, "strand": "+", "frame": 1, "gene": null, "product": "type I restriction endonuclease subunit R, EcoR124 family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6", "UniRef:UniRef90_UPI00280A69CD" ], "nt": "TTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGATGAATTTATTGGCAAGGAAATCTTAAACGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACTGAATTGTTGAAAACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTCCGTGGAAAGACAATTACTGAAATTAACAATTTAAGAAATGAAATATCTTTAGCTTCTTCTTCATCGCCTGAATTAAGAAGCAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACAAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAAAAGAAAGAGCTTGACAAAATTATTAGTGAAGAAAATTTAGATTTTGATAAGACCCATAAATTTATGGATGATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCATTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGGCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAA", "aa": "MFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDDSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI", "aa_hexdigest": "dc00a72271e9cca2ee211ea66ebf6ae9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 738121, "stop": 739068, "observations": { "mutation": [ 738379 ], "stop": [ 738379 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280A69CD", "query_cov": 1.0, "subject_cov": 0.5456852791878173, "identity": 0.991, "score": 402.0, "evalue": 7.18e-139, "gene_start": 1, "gene_end": 215, "reference_start": 180, "reference_end": 394, "reference_sequence": "MQLNKPDRDFLDGAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTLWVDKKLREHGLIQAFSRTNRILNDKKVYGNIVCFRNLEKETDQAISIFGDGEASGIVKIKNFVDYYYGYNDSQQVHHNGYCDYIQKLNNEFPLDNWNVISEQKNKEFIILFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDNSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI" }, "paralog": false, "identity": 0.9936507936507937, "score": 620.0, "evalue": 4.37e-227, "description": "Internal stop codon at 738379. Nonsense mutation around 738379.", "psc": { "uniref90_id": "UniRef90_UPI00280A69CD", "product": "type I restriction endonuclease subunit R, EcoR124 family", "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280A69CD", "UniRef:UniRef50_A0A412PBS6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6" ], "product": "Type I restriction endonuclease subunit R" } }, "id": "LMJBGEANGM_1274", "locus": "GCA013368015_01274" }, { "type": "cds", "sequence": "contig_1", "start": 739132, "stop": 739758, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAAGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGATGAAGAAGCAGATCTTGAATTTATTTTCGATCAAGAAAATGACAATTTTGCAATTATTAATCATAATAAAGTTGTCGTTGGTGATCCAATAACAAGCGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCATTAAGTGATGCTAAAAAAATAGATCCAAATGTAGAAATTGGAGATTCTCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGACATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGCGAAGTTGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGACGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAATCAAAGAATAATTTCAAAATTAAATCCAGGCGATTGA", "aa": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNVEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGD", "aa_hexdigest": "fa2b76befdc2bab8bbaee7ee6eb46e62", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 130, "aa_cov": 0.625, "hmm_cov": 0.9153846153846154, "evalue": 5.6565494849613775e-21, "score": 70.53514099121094, "start": 24, "stop": 153 } ], "seq_stats": { "molecular_weight": 23793.965500000017, "isoelectric_point": 5.478733253479004 }, "id": "LMJBGEANGM_1275", "locus": "GCA013368015_01275" }, { "type": "cds", "sequence": "contig_1", "start": 739966, "stop": 740772, "strand": "+", "frame": 1, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "COG:COG0195", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J8W2", "UniRef:UniRef90_D1J8W2" ], "nt": "ATGGAAGAAATTGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGAGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGAATACATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGCAATATCACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTAGCAATAAAAATGCTTCAAATAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTACGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAA", "aa": "MEEIGAIMGRDSERISTISRKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTISNKNASNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW", "aa_hexdigest": "c9ae95f0c23118050856c8f2f044a416", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 739666, "stop": 740772, "observations": { "mutation": [ 739756 ], "stop": [ 739756 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W2", "query_cov": 1.0, "subject_cov": 0.4908424908424908, "identity": 0.993, "score": 499.0, "evalue": 7.7e-174, "gene_start": 1, "gene_end": 268, "reference_start": 279, "reference_end": 546, "reference_sequence": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNAEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGDWIDVVIEEVKEENSNAQIIVSSVESRLLQKLFEIEIPEISQGLISIVNIARIPGERAKVSIKKSNDAPETMEEIGAIMGRDSERISTISKKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNASNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW" }, "paralog": false, "identity": 0.9918478260869565, "score": 710.0, "evalue": 2.23e-259, "description": "Internal stop codon at 739756. Nonsense mutation around 739756.", "psc": { "uniref90_id": "UniRef90_D1J8W2", "gene": "NusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_D1J8W2", "cog_id": "COG0195", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W2", "UniRef:UniRef50_D1J8W2", "COG:COG0195", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W2" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "LMJBGEANGM_1276", "locus": "GCA013368015_01276" }, { "type": "cds", "sequence": "contig_1", "start": 740766, "stop": 741023, "strand": "+", "frame": 3, "gene": "ylxR", "product": "YlxR domain-containing protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "SO:0001217", "UniRef:UniRef50_D1J8W3", "UniRef:UniRef90_D1J8W3" ], "nt": "ATGGTAAAGCCTATACAAAGAAAGTCAATTGTTGATAATAAAATATACGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTCTAAACGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAAAATATAGATATTCAAAAATACAATGAAATAAGGAAAGAGGTGGACGAATGA", "aa": "MVKPIQRKSIVDNKIYDIDLLLRFSKDKNNLISFDPYKKNPGRGAYCLNDSEQIEILFKRRLLNKAFKQNIDIQKYNEIRKEVDE", "aa_hexdigest": "8ccb84de81cd0d1bd880b0995cd8ef72", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8W3", "query_cov": 1.0, "subject_cov": 0.85, "identity": 1.0, "score": 171.0, "evalue": 4.08e-54, "valid": true, "gene": "YlxR", "product": "YlxR domain-containing protein", "uniref50_id": "UniRef50_D1J8W3", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_D1J8W3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W3" ], "product": "YlxR domain-containing protein" }, "genes": [ "ylxR" ], "id": "LMJBGEANGM_1277", "locus": "GCA013368015_01277" }, { "type": "cds", "sequence": "contig_1", "start": 741020, "stop": 741763, "strand": "+", "frame": 2, "gene": null, "product": "Translation initiation factor IF-2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "nt": "ATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCATTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCAGAATTTTCTCTTAAAATAAATAAAAACCCTACTGAAATATTGACTTATTATTTTAAAAAAGGAATAATCAAGACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGACCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGGCATGTTGACCATGGCAAGACTACATTAATTGATAGAATAAGACATAGTAATGTTACTGAAACAGAATTTGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACCTTTTTAGATACACCCGGTCATGAGGCTTTTACCGAAATGCGTTCAAGGGGAGCTAAAATTACTGATATAGTAGTTATTGTAGTTGCGGCAGATGATGGAGTTAAACCTCAAACTATAGAAGCAATAGACCATGCAAAAGCAGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAGGGAGCCGATATTGAAAGAATAAAAAGCGAACTTGCTGATAACAATGTTTTATGTGAAGAATGA", "aa": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKINKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEE", "aa_hexdigest": "602c93818d65bf5a6bd2e13b8c4b98da", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0025CBB306", "query_cov": 1.0242914979757085, "subject_cov": 0.8322368421052632, "identity": 0.534, "score": 263.0, "evalue": 1.58e-84, "valid": false, "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_A0A357QY70", "kegg_orthology_id": "K02519" }, "pscc": { "uniref50_id": "UniRef50_A0A357QY70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "product": "Translation initiation factor IF-2" }, "genes": [], "id": "LMJBGEANGM_1278", "locus": "GCA013368015_01278" }, { "type": "cds", "sequence": "contig_1", "start": 741839, "stop": 742837, "strand": "+", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_D1J8W4" ], "nt": "TTGCAAGCTGATTTACTTGATTTAAAGGCCAATATAAATAGAGATCCTATTGGGACAATTATTGAATCTAGAATTGACAAAGGACGTGGAGTTGTTTCAACTGTTATTGTTGAAAGTGGAACATTGCTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTCTAACCTCTATTGATGGCAAACCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATCACTGGTTTAAGCAAAAACCCTCAATCTGGTGATAAATTTGTAGCTATTAGCGACGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCATATTTAGATAAATGCAAAGAATTAAATGAAAAAAATGCATTTTCTATTCAAGATGGAATAAAAACTGTAAATGTCATTGTTAAAACCGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATAAGAAATGATGAAATTGCAGTTAATGTTATAAGAGCAACCTCCGGCGAAGTAACGAAAGCAGATATCTTGCTTGCTGAAACATCTAATGCAAGAATTTATTTATTCAACAATACAATAAATAACGAAATTAAAAAGGCGGCTGAATTAAAGAATATAAAAATCTTTAAACACAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTACGAAGAAAAATTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGTCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAAGTATACAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAGGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAG", "aa": "MQADLLDLKANINRDPIGTIIESRIDKGRGVVSTVIVESGTLLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN", "aa_hexdigest": "e6f67233f3ec7882845ae4b5e734144f", "start_type": "TTG", "rbs_motif": null, "genes": [ "infB" ], "truncated": "5-prime", "pseudogene": { "start": 741539, "stop": 742837, "observations": { "mutation": [ 741761 ], "stop": [ 741761 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W4", "query_cov": 1.0, "subject_cov": 0.5487603305785124, "identity": 0.997, "score": 634.0, "evalue": 5.29e-225, "gene_start": 1, "gene_end": 332, "reference_start": 274, "reference_end": 605, "reference_sequence": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKISKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEEWGGNYQFVYGSALKNSNINELLEAIFLQADLLDLKANINRDPIGTIIESRIDKGRGVVSTVIVESGTLLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN" }, "paralog": false, "identity": 0.9976851851851852, "score": 832.0, "evalue": 2.02e-305, "description": "Internal stop codon at 741761. Nonsense mutation around 741761.", "psc": { "uniref90_id": "UniRef90_D1J8W4", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W4", "UniRef:UniRef50_Q98R05", "COG:COG0532", "COG:J", "KEGG:K02519", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" } }, "id": "LMJBGEANGM_1279", "locus": "GCA013368015_01279" }, { "type": "cds", "sequence": "contig_1", "start": 742824, "stop": 743057, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0006364", "SO:0001217", "UniRef:UniRef50_A0A448ZXY5", "UniRef:UniRef90_D1J8W5" ], "nt": "ATGAACACAATTAGTCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATATTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTGGCAAAGAATTGA", "aa": "MNTISHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKN", "aa_hexdigest": "3fcfd61932e74de4b902676108094175", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 742824, "stop": 743165, "observations": { "mutation": [ 743055 ], "stop": [ 743055 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W5", "query_cov": 1.0, "subject_cov": 0.6814159292035398, "identity": 0.987, "score": 152.0, "evalue": 1.3e-46, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MNTINHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKNWKYKVLPELIFEIDTVEEHAAKIDKILNKIEEEKNN" }, "paralog": false, "identity": 0.9823008849557522, "score": 218.0, "evalue": 4.65e-75, "description": "Internal stop codon at 743055. Nonsense mutation around 743055.", "psc": { "uniref90_id": "UniRef90_D1J8W5", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A448ZXY5", "cog_id": "COG0858", "cog_category": "J", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W5", "UniRef:UniRef50_A0A448ZXY5", "COG:COG0858", "COG:J", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXY5" ], "product": "Ribosome-binding factor A" } }, "id": "LMJBGEANGM_1280", "locus": "GCA013368015_01280" }, { "type": "cds", "sequence": "contig_1", "start": 743186, "stop": 743458, "strand": "+", "frame": 2, "gene": null, "product": "Glucose-inhibited division protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "nt": "ATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGCGGTTCTGCAAAAGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGTTAAATACATCTAAAGGCCCAGCAGTTTGA", "aa": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAV", "aa_hexdigest": "1a1f7e54074d6f0611af994f07d089c1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AE77E7", "query_cov": 0.9555555555555556, "subject_cov": 0.8113207547169812, "identity": 0.733, "score": 130.0, "evalue": 8.79e-38, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A1T4L5B9" }, "pscc": { "uniref50_id": "UniRef50_A0A1T4L5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "product": "Glucose-inhibited division protein A" }, "genes": [], "id": "LMJBGEANGM_1281", "locus": "GCA013368015_01281" }, { "type": "cds", "sequence": "contig_1", "start": 743537, "stop": 744865, "strand": "+", "frame": 2, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0008033", "KEGG:K03495", "SO:0001217", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_D1J8W6" ], "nt": "TTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGTGTTGGAATTGAAACACTTGAACATGGTAAACTATATTCAAAAGTTGTTATTATGACAACTGGTGTTTATATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCCGATGGAGAAAAGACAAGCTCAACACTTTCAAAAAATTTAAAAAAATATGGTTTTGAAATAATAAGATTAAAAACTGGAACACCTTGTAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTGGAAAAAAATGAACTTTGTTTTTCAAGTCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTTGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAACCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCCGCTCAAGGATTAATAGCAGGAATTAACGCTGCTAATAAACTTGATAATTTGGAACCATTAATCATAAAAAGAAATGACGGTTATATTGGCGTTTTAATTGATGACATTGTAACTAAAGGCACAAATGAACCTTATAGAATGTTAACAAGTAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAGACAAAGAAGCACATGATAGGATTGAAGAAAAATATAAGATTATTGCTGAAACAGTAGAAAAATTAAAAAATGAATATGTTTCTTCTAAAAGTGATGTTGGTCAAAAGTACAATGTAACAAATGGTATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGTTCAAATTTTCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCGGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGA", "aa": "MIQDEASDLIVEDGTCVGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMIDKEAHDRIEEKYKIIAETVEKLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDY", "aa_hexdigest": "98da9c430ca82d94d762d71466d93bbb", "start_type": "TTG", "rbs_motif": null, "genes": [ "mnmG" ], "truncated": "both", "pseudogene": { "start": 743237, "stop": 745006, "observations": { "mutation": [ 743456, 744863 ], "stop": [ 743456, 744863 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W6", "query_cov": 1.0, "subject_cov": 0.7293729372937293, "identity": 0.986, "score": 861.0, "evalue": 8.1e-313, "gene_start": 1, "gene_end": 442, "reference_start": 118, "reference_end": 559, "reference_sequence": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAVWSLRAQIDKEKYSEIILKDIEKQKNITLIQDEASDLIVENGTCIGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVERLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDYWSVDNLATEARQKLDKIRPETIGQAARISGINPADIQMLIFYLNNKR" }, "paralog": false, "identity": 0.9881154499151104, "score": 1145.0, "evalue": 0.0, "description": "Internal stop codon at 743456, 744863. Nonsense mutation around 743456, 744863.", "psc": { "uniref90_id": "UniRef90_D1J8W6", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W6", "UniRef:UniRef50_Q98QV8", "COG:COG0445", "COG:J", "KEGG:K03495", "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" } }, "id": "LMJBGEANGM_1282", "locus": "GCA013368015_01282" }, { "type": "cds", "sequence": "contig_1", "start": 745009, "stop": 745440, "strand": "+", "frame": 1, "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "COG:COG1576", "COG:J", "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_020002370.1", "SO:0001217", "UniParc:UPI00036F4328", "UniRef:UniRef100_UPI00036F4328", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_D1J8W7" ], "nt": "ATGAAATTAAATATTGTAGCAGTAGGTTCTCTAAGCAAGGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTGATTGAAATAAAAGAACAAGTTAATAAAAATATTGATTTAAAAATAGCTGAAGAAACAAAATTAATCCTAGAAAAAATTCCAAAGAACTCTAAAGTTTATTATTTGTCATTAAGAGGCAAGAAACTTGATAGTGGCGAATTTTCGGATTTACTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTAACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAA", "aa": "MKLNIVAVGSLSKEYKTIYDQYLKKISFFCSINLIEIKEQVNKNIDLKIAEETKLILEKIPKNSKVYYLSLRGKKLDSGEFSDLLIEDNITFVIGGSNGVDETYFKNKICFSDLTFPHQLFRIMLIEQIYRGFSIKNNIKYHK", "aa_hexdigest": "715fa86346ee70e9b7f8188ecbabc58e", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00036F4328", "ncbi_nrp_id": "WP_020002370.1", "uniref100_id": "UniRef100_UPI00036F4328", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036F4328", "RefSeq:WP_020002370.1", "UniRef:UniRef100_UPI00036F4328" ] }, "ips": { "uniref100_id": "UniRef100_UPI00036F4328", "uniref90_id": "UniRef90_D1J8W7" }, "psc": { "uniref90_id": "UniRef90_D1J8W7", "gene": "RlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "cog_id": "COG1576", "cog_category": "J", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [ "rlmH" ], "id": "LMJBGEANGM_1283", "locus": "GCA013368015_01283" }, { "type": "cds", "sequence": "contig_1", "start": 745895, "stop": 746014, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTACGACTATACTTGGGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATATATCGCAATGATGGCAAAATGA", "aa": "MLRIDHHPVDPDINYDYTWEDATYAAAAEQVGYIAMMAK", "aa_hexdigest": "a8aae5d778d2622d7d44e34280a6413b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4486.9666, "isoelectric_point": 4.3189905166625975 }, "id": "LMJBGEANGM_1284", "locus": "GCA013368015_01284" }, { "type": "cds", "sequence": "contig_1", "start": 746541, "stop": 746930, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAGAAAATTGAAGAAAGAATTAAAGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATTGGTGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTACCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCCAACTTTAAAGAACGTTTAGAATGTAGAGAATACTTGGATAATAATTGTTTTGATGAAGTAATAAGAATCGATCACCATCCAAATGATGATGATCTAGATGCTTCATTAAGATGA", "aa": "MNKISAEKLAIFKKIEERIKAHKNIVIYHHIRPDGDCLGSQFGMKNLIKVNFPDKTVYTIGDSKGIYSFLEFKMDKLPLEKLDDSLAIIVDANFKERLECREYLDNNCFDEVIRIDHHPNDDDLDASLR", "aa_hexdigest": "0c2a38fe4e5df54da82c9499dd708430", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.751937984496124, "hmm_cov": 0.76, "evalue": 1.5197733166320953e-12, "score": 43.433292388916016, "start": 24, "stop": 120 } ], "seq_stats": { "molecular_weight": 15013.060199999985, "isoelectric_point": 5.709670066833497 }, "id": "LMJBGEANGM_1285", "locus": "GCA013368015_01285" }, { "type": "cds", "sequence": "contig_1", "start": 747159, "stop": 747326, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATATTAATATAAATACATATATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTAGGCTCAATTGACAATACAAGGGCATGA", "aa": "MFDININTYISQNMKIANDVVSFYFPLSEQIKLGIKDPLKANRPFVLGSIDNTRA", "aa_hexdigest": "54c5c29184ba42abae513926e9dce02a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6262.1726, "isoelectric_point": 8.0980188369751 }, "id": "LMJBGEANGM_1286", "locus": "GCA013368015_01286" }, { "type": "cds", "sequence": "contig_1", "start": 747938, "stop": 748480, "strand": "-", "frame": 2, "gene": null, "product": "Phage abortive infection protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6", "UniRef:UniRef90_D1J8X0" ], "nt": "ATGACATCAAAAGAAAAGAAAATGAAAATAAAAAATGTTATATTAAACATTATTGGTTTTCTAACCGTTGCTCTCTTTCTTATTATTGCTATCTTGCTATTTTTAGCAGCAAATGGCATTATGGGAACTATATCTAAAAAATCAAGTATTGTTTGTTATGTTTTTGGTGCAATCTTCTTAGCTATATTCATTTTAATTGTAATTAAAATGATATTAATATTAAAAAAAGAAAATGTATATATAAAAAATGCAATCGATACAGATAAGCTCTTTGCAAATGTTTCTCTAAGCCCAGAAGAAAATGAAATTCATAAGCAATTTATTGAAAAATTTAAACAATATCAACAAAGTAGAAACATATACTTCGGATATTTATTTACTAAAGCATTAAGCTCATATAAAAGAGATAATATCGATATTTCAGACCACGAAATAAATTCATTAATTGAAAAAATGATTATTGACTGCCATAACGAATTTGGAATTTTTGATGTCTATTTAGCAATTGATTTAGCAAATTCATTAAATAAAAAGCTTGTTTGA", "aa": "MTSKEKKMKIKNVILNIIGFLTVALFLIIAILLFLAANGIMGTISKKSSIVCYVFGAIFLAIFILIVIKMILILKKENVYIKNAIDTDKLFANVSLSPEENEIHKQFIEKFKQYQQSRNIYFGYLFTKALSSYKRDNIDISDHEINSLIEKMIIDCHNEFGIFDVYLAIDLANSLNKKLV", "aa_hexdigest": "c115dfb99521affcc1d948c5bac5ac88", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J8X0", "query_cov": 1.0, "subject_cov": 0.8450704225352113, "identity": 0.9890000000000001, "score": 328.0, "evalue": 5.58e-113, "valid": true, "uniref50_id": "UniRef50_A0A501X7R6" }, "pscc": { "uniref50_id": "UniRef50_A0A501X7R6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6" ], "product": "Phage abortive infection protein" }, "genes": [], "id": "LMJBGEANGM_1287", "locus": "GCA013368015_01287" }, { "type": "cds", "sequence": "contig_1", "start": 748588, "stop": 749118, "strand": "-", "frame": 3, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:6.3.4.21", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_D1J8X1" ], "nt": "ATGGCAGATAGGGCAGATCATTATATAAATCAACCAAACGACTCATATGCAATAAGAATAGGTGGTATAGAAAATCACTCGACTTTTCTAAGCTCAAATTTTGATCCAAAGCATACTTTTGGAAGCATGCCTCATGCATTAATTCAAATGTTTGACGGTGATATTGTAGCTGCTTGCAAAGCATATAAAAATGTTTTTCCAAATGATGATCTAGTTGCCCTAGTTGATTTTCACAACGATGTTATAAGCGATTCATTAAAATGTTTAAAGGCTTTTGGCAAAGACTTAAAAAGCGTAAGAATAGACACATCGAAAGCGCTTATCGATAAAATGTTCAAGGAGGGTGAAGCGGAATTTGGCGTTACACCAAATCAAGTAAAAAGGTTAAGAGAAGCATTAGATAATAATAACGGAAAGCATGTAAAAATAACTGTTTCAAGTGGATTTGACGCTCAAAGAATTGCTGAATTTGAAGCCGAAGAGACTCCTGTTGACACATACGGCATCGGTGCAGCGTTGCTAAAAATTTGA", "aa": "MADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKI", "aa_hexdigest": "75d1c7c3d0104449e47eb3857299e092", "start_type": "ATG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 748480, "stop": 749418, "observations": { "mutation": [ 748590, 749157, 749217 ], "stop": [ 748590, 749157, 749217 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X1", "query_cov": 1.0, "subject_cov": 0.5238095238095238, "identity": 1.0, "score": 355.0, "evalue": 1.03e-121, "gene_start": 1, "gene_end": 176, "reference_start": 125, "reference_end": 300, "reference_sequence": "MKKINLKDYISIYFYKTQKISKKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGIWEGIIDGILARQSSIATNAYWAIKAANGKKIVSMADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKIWVNFSADATRLNGHNIAKAGRGYLKNPQLITYKEAK" }, "paralog": false, "identity": 0.9903846153846154, "score": 613.0, "evalue": 8.66e-226, "description": "Internal stop codon at 748590, 749157, 749217. Nonsense mutation around 748590, 749157, 749217.", "psc": { "uniref90_id": "UniRef90_D1J8X1", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "cog_id": "COG1488", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X1", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "COG:COG1488", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "LMJBGEANGM_1288", "locus": "GCA013368015_01288" }, { "type": "cds", "sequence": "contig_1", "start": 749215, "stop": 749490, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02749.22" ], "nt": "ATGAAAAAAATCAATTTGAAAGATTACATTTCAATTTATTTTTACAAAACTCAAAAAATATCAGAAAAGTTCAAAAAAGATCAAAACATTACTCTACAATTCTTTCAAAGACATAATGATGTCAAATTATGTGGAATAAATGAAGCAATCGATATTTTAAAACAAAACACAGATATTTCTAAATACACCATAAGATACATGCCAGAAGGCAGTATAATTCACAATAGAGAAGTAGTTTTAGAACTTGAAGGCCCTTATTATGAATTTGGTATATGA", "aa": "MKKINLKDYISIYFYKTQKISEKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGI", "aa_hexdigest": "25c5857975b38a372459a90621109c0e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02749.22", "name": "QRPTase_N", "length": 79, "aa_cov": 0.8681318681318682, "hmm_cov": 0.8481012658227848, "evalue": 3.9706002207593366e-11, "score": 38.595455169677734, "start": 12, "stop": 90 } ], "seq_stats": { "molecular_weight": 10912.484899999996, "isoelectric_point": 8.625950431823732 }, "id": "LMJBGEANGM_1289", "locus": "GCA013368015_01289" }, { "type": "cds", "sequence": "contig_1", "start": 749815, "stop": 750072, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2", "UniRef:UniRef90_D1J8X2" ], "nt": "ATGGTAAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCAATGATAGCTGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCATTAAGAAAATGCTATTTGATTTCGCTAATTCCATTATTGTTTTTTGTAGCATTGGTAGCAAGCTTTGTATCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAACGTTGCTATAGCAGGATAG", "aa": "MVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG", "aa_hexdigest": "581490f79f53323a0d0b60fb7875800c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 749647, "stop": 750072, "observations": { "mutation": [ 749728, 749785 ], "stop": [ 749728, 749785 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X2", "query_cov": 1.0, "subject_cov": 0.6028368794326241, "identity": 1.0, "score": 155.0, "evalue": 3.73e-47, "gene_start": 1, "gene_end": 85, "reference_start": 57, "reference_end": 141, "reference_sequence": "MAKNQDKKMLSPEEKEIENIKKISKNGWHFSYWSLFFILGVIAILIWAVESESSSSMVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG" }, "paralog": false, "identity": 0.9787234042553191, "score": 262.0, "evalue": 7.72e-92, "description": "Internal stop codon at 749728, 749785. Nonsense mutation around 749728, 749785.", "psc": { "uniref90_id": "UniRef90_D1J8X2", "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X2", "UniRef:UniRef50_D1J8X2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9565.66899999999, "isoelectric_point": 9.555197715759277 }, "id": "LMJBGEANGM_1290", "locus": "GCA013368015_01290" }, { "type": "cds", "sequence": "contig_1", "start": 750243, "stop": 750443, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTACTTTACCTATAATACTTGTAATTGTTGGTTATATTTTAATTACATACATATTAACAACGTCAATACTATTGATGAAAAAGCCACAATTAATAACTAAACGCCACACTTTAATTTCGCAAGTTGCAATAGCGTTTATTCCAACATTAAGTGTTTATGCTATAACTTCATTGGATAATTATTTCATCCTATGA", "aa": "MKITLPIILVIVGYILITYILTTSILLMKKPQLITKRHTLISQVAIAFIPTLSVYAITSLDNYFIL", "aa_hexdigest": "022642b7f95753253b7474b93d30f264", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7463.145299999999, "isoelectric_point": 9.777226829528807 }, "id": "LMJBGEANGM_1291", "locus": "GCA013368015_01291" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Metamycoplasma hominis] [strain=SP10291] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TTATTTTGCTTTTGCTGTAAGCTTTGGTTCAACTTTTTGTTTAAAGAATTTTGTTCTTTGGAAATAGTGAACAAATATATGTTGCAATATTAATATAAATGCTCCAACAATTCAATAAATTTGTAGTCCGGCACTAAAGAATGCTCCAAAGATTATAAATACTAATGAAATAATATTTGACATACGATTTTGTTTTTTCATTGCTGCTTTTTGATAAGCATCAACACGTAATGACTTCTTTTTCTTTCAATTTAGAATCTTTGGCATATATTGCGATAGTGCTTGCATACCGACAGATATTAAAATTATTGGTAGATATATGAATTCTTTGTTAGAAAATACCCGGTTTAGTGAGTTTGCTGATAATTGAATTGAATATCAGGTTGCTTGTTTTATTTCTGGAGAGGTATTTATAACTCTAAATACAACAATTAATATTGGCAATGCAATAAACTGAGGCCAAATCTGTGATCAAGGAGAGATTTTTTCTTTCTTATATAATTCACTAATTTCAGCTTGCTTACGTTGCATCATTTGTCTGTCGCCTTTGTAAGCAGCATATTTGGATTCAATTTTGGCTTTCTTTTGGTTGAATTCTTCCATTTTTGCTTGACTAAACAAAGACTTGAACGAAACACCAAATACTACTAAACGAACAATTATAATAACAACTATTAATGCTAATATAATACTTCATCCTGTTGTTCCAAGTCCTTTTATAATTGAGTCCATAAATAAGTATATAGGTTGAACAAATATACCATAAAATGGTCCTTGTGCTCAATATTCTTTGTATGTTGAAATAGGTTTTTGACTCAATATTGCGCTATTATTAAATATTGTGTTTGTTCAATATTCAGAATTGTAATTTGAAGCAGGACTTAGACTTGATTTTCTATATACAAAACTACCGTCATTTGTTTTAGAAGGTAATTTTACATAATTTACATTTGATAAGTATTGTTGGAAAACACTTTTAACAAAAGTAAATAGTTGAAGTAACTTTCTTCCTTCATCTTCGTTGGTTAAAAGAGTTTTATTTTTTGCTTTTTGTTCATAGCTTCTTATAAATTGATTTCATTTTTCAATTATTTGGTTAGCATTTTCACTACCATTTAATTTTAAAGCTTCTTTAAAGATGTTAACCTTATCATCTTTAAAGGTTTCCAAACTTGCTCATTTTTGTAATGTATTTGAAACAAGAATTTGAAAAATATCTCTTAGAACAATTTCATCAACTGATGAACCACCAGTTAACTTTCCTTCTACTATATCATAGTTAAATTCTTTTATTTTTGTTCAATCTGTTGGTATAATGCCATCCTTATCTTTTGGCTTAGTGTCAAACGATAAAGAATTAGGAATTAGAATTTCACTGAATTTGGTTAATGGAGTGTAATTTTTGGTTTTGTAATTTCCCTTTTCATCACCCAAATTAAAGTAGGCATAATTTCCATTTACATTGTAGATGTAGCCACTTTTATCAGATGTTCCTTTTTCTGTTATATTTGTTCAATCTTCATCATGAGAAGATAGAACTTTTGCTTTGTCGTTTTTTGGCATTTGCAATTGCAATCCAAATGAACGAGAACCATATGCGCCATAATTAGGATCTCTCTTCCTTAACTCATTAATAGCATTAGGTTCTTTTAAAGCAAGATATGTATTGGCGATAATATTTTTATCATTTATTTTGTTTGGTAAGGTAAAGAAATTACCAGTGCTATCGTATCTATAAGTGACAATATTTGGTGAAATGTCACTCTTTTTAGAATATAGCTCTTGTCCAGAACCGACTTTATTACTTGATTTTACAGTAAATGATTGTACACAACCAACTAAACCGATAGCCAAAAATAATATAACTATGACAATTTTTATTCATTTTCAAACTTTTAACCAAATATTTTTCGGTGTTTTGTTTTCTGGTTGACGAACTCGAAAGTCTTTATAGAATTTAGAGACATCCTTATTTGCCATCAGCGATCCTTTCATAAAGTTTTTTTACTTCTTTAACTTTTTGATTCCAGTCAAGACTCAAGAAGCCTTTGCGAGCAATAATCACAGTTTCGTAATTTAAAGTGCTAAAATCAATGTTTCTTAGAATTGATCTGATTTGATTTTTGTAATGATTTCTCAAAACAGCATTTGCAAATTGCTTAGGGATTGAAATACCCACTTTAAAGAAATCGGATTTGTTAAAATAAATTATTAAATTTCTTGAAATCACTTGATTGTGAGAATCAAGAATTTTTTGAAATTCCCAATTTTTTTTAACAACGTTATTTTTATTTATCATAATTTAAAAATATGCAAGGTATTAGACTTATTTATTGTCTGATACAGTTAATGTATATCTACCTTTTGCACGTCTAGCTGCTAAAACTTTTCTACCATTTTTAGTTTGCATTCTAGCCATAAAACCATGAACTTTAATGTGTTTGCGTTTTTTTGGTTGGTATGTTCTTTTCATAACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGTATATAAAATACCTAAGAAAAAAAATTTAATATTTTTTATGTTTTTAATGTAAATTAAAAGAAACACAAAATTCAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAACCTCTTATTTTAAAAACAAAAGCCCTAAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGTTTATTAATTTATTAATTTTGAATTTTAAAATATAATAGAGGTATGAAAACTACAAATCCCTCTGAATTAGATTTAAGAACACATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCTTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGACGTTTTAACATATATTAAGAATAGTCATAACGAAAGTATTAAAAGAGCAATTAAAAACGTTTATGGTGAAGAATATAATTTGGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTGCAAGATAGCAATAATGTTGAATCTGCAACTAATTCATTGACTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAACATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTTATTCATGCTTCAAGTGGGCTTGGTAAAACACATCTATTACATGCTATCGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTAATGTCTTATGATTGCCTAATGTTTGATGATGTTCAACAATATGGTAATCGTGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAGCCGGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATATCTTGAAATTTAAGTTGCAAGAAAACGGTATAAATCCGGAACTTTGAGAGGAGGAAAGCTTAAAATTTATTGCTAGAAACTTCTCTTCATCAATAAGAAATATTGAGGGTGCAATTAACAGAATAAAATTATTTCAAGATGGTGACGACGGCTTTTTTACATATGATATTAATACAATAAAATCAATATTTAACAGCATGACTCAAGTTGCTGAGAATATTACCCCCGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGATGTGGTTAATGCCCGTAGAATAGCAACATATTTAGTGAATAAAAATTTTAATTATACATTGCAAGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCGCTTAATTGAATTGAAAAAAATGCTAACAGTAATCCAACGCTTAAATTAGCATTGCAAAAAATAGAAAGCAATCTTAAAAAAATTTCGTAATAGGTAGGTATAGAAATGAAATTAAAAATTGATAAATTGTTATTGGATAATGCAATTGAAAGAGTATCAAAATCCGTGGATGCTAATCCATTTTTACCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAGCATGAAATAGAAACATCGATTGATGCCCAAATTGAATTGCCTGGAATTGTATTAGTTGAACTTAGCTTATTTAAAAATATTATTAAAAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATAAGTACAAACAACGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATTGATTTTGCATGTTATGGGGAACAAGTAAAAATTAACTGAAATGATTTTAAATCTTTAGTTCGTGATGTTATTTTTGCTGCTTCAACAAACGAAGTTAGTCTAATCTTGTGCTGTGTCAATATATCGGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCCCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAACTTTGATTACAATGGCGACGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAGTCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACTGAACTTAAAATAACCAAAAAAGAACTAAATAACTTATTGAATAAGGCATCAGTTATTATTAATGAAAACTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAACCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGCGAAGAATTGAAACTCGCATTAAATAGTAGATTCTTAAAGGATGCTATTTCTGTATATGAAGATAATTTGAATATTTTATTTACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGTCCAACGGGCGGAATGGCAAAATATTTTGTTCAGGTTCATAGAATATTAATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGAATAGATGACAATGTTTATTTAATAAAAGAACAAAAATAAAAATATTTTTTATAAATATTATAAAATATTATAATTATTAAGCTTAACAATAACATAAAGGAGAAATCATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAGTAGTTTTTTATAAAAATAATATGAAGTTGCCTAGGCAACTTTTTATTTTAAAAAAAATTAAGCAGTTGGCTTAATTTTTATTGTTTATAAATTTGAATAAATTAATAGAAATTTCTTTTGTAATTTTTTCAGCTCTTATATTTAAATCTAGTTCATTGCTTTTTAAAAAATCAGAAATTTTTTGTTTTAAATTGCTTGGAAAATTATTTAATAAAGTTTTACGCTTGTATTGAAACATTTGTTTAATAAATTTCAAGATTTCAACTTGATTGTTTCATATAAAATCATTATTTTTTATTAGATTTAGTTGAATAACTGCCGAATCAACTTTAGGAGCGGGATTAAATTCTTTTGCCTTAACAGTGAATAAATATTTAGGTTCATAGAGAAGTTGTATTGATGCTGCTAATTTACTATATTGAGAATTACCGGCTGTACAGCATATTCTATCCGCTACTTCTTTTTGAACCATTAATGTTATTTTATTAATGTTTTTAGCGTTATCTAATAATTTAAAAATTATATCGCTTGTTATATTATAAGGAATGTTTCCAATTACTTCAAAATCAGTATATTTTAATAAATCTGTTTGTAAGAAATCTTGGTTCAATATTTCAACATTGCTTGAATAATTTTTTTGTAATAAAAATTCGTATAATCTTTTATCAAATTCAATACATACAAGTCTATTTACAACATTTGCCATTTGATCAGTTATTGCGCCCATTCCTGGGCCAATTTCAATAATATTTTTGCCTGCAACGTTCGCAACTTTTACAATCTCTTTTTGAATGTTTTTATTGCTTAAGAAATTTTGGCCCAATGATTTTAATGCTTTAATCATAGATTTTAAAAACCTTTCTAACATTCTTTTTAATTTGTTCAACTAAAACATCAATATCAATTTGTTTTAAATTAGCAATGTATTCATATGTGTATTGAACAAAAGGGCTAATGTTGGGTTTTCCTCTCATTGGAGTTGGGGCTAAATAAGGTGTATCAGTTTCACAAAATATTCTATCTAAAGGAATATTTTTTACTGCCTCTTGTAAAGGCTTAGCATTTTTAAAAGTAACAACCCCACTTATTGAATAATAGATATTTGAATATTTCAAACATTTATTTAAAAAACTAAGATCCCCGCTAAATGAGTGAAATATAAAATTAATGTCTTTATATTGAGGCAAGGTTATTATATCTAAAGAATCATCTAAAGCATCTCTAATGTGCATAATTACTGGTAAATTATGTTCTTTTGCAAAATCTATTTGGGCAATAAATCCTTGTTTTTGAATTTCTTTGCTAGGCGAATCATCATAATGATAATCAAGGCCCACTTCTCCAATTGCAACAACATTTGGTTCATATATCTTGTTTAAAATTTCTCTATCTTGCAAACCATTTGATAATGTTGGATGTATTCCAATAACAGGATAAACAAAATCAACTTTTTTAGCAAGTTCTATTGCTTGTATCGAATCATCTTCGTTTGTTCCACATACAAAAAGCTTTTCCATATTTAGGTTATGCCAATGATTAACTATTTCAAGAGGATCAGAATAGTATTCGGTTGATGGGTGGGTGTGTAAATCGATAAATTTCATTATAGTTCTCCTAATATATCTTTAAAAATATCAATTATTTTTAATTCCCAAATTGCTTTTGATTGCCTATATTCTAGGTTATTATTAGATTCCAAACAATCTGAAATTACCTTGAATGAATAAAATGGTTTGCCAAAAATAAATGATGTATGTGCCAATGAACAACATTCCATATCGCCTAATAAATTATTTGTATTTCAATCTAATATATCTGAATTTATTATGCTTTTATTAATAAAACTGTCGCCGGTTATAATATTGGCTTTTTTAAAATTGCTGCAAATTTTTAGATCTTGATTGCAATGAAATACATCTTGCAAACTAGGCAATTGCCCAATTTTATAATTAGGTAAGGCGGTTAAATTAACGTCATAATATTTGCAATTTTGTATGCAATAAATATCTCCAACGCTACCATTGAAAATTGATATAGCAGGTCCAATGTTAATTACTTTTAATATATTGAACTTAGTCAATAAATAACTTAGCATTAAAGCAGAATTAGCTTTTCCAACTCCACAGTGCGCTAACAAAATTTGTTTATTGTTTCAATTTAATTCATAAAGATTCTGAAAATCGTTTTCAAAATCAATTTTTATTTGTTCTAAATGCTTGCTATATTTTAGCCTTAATTGTTCAGTTTCTAGTGGTTCGGCAAAAATAAATAAATAAAATTTCTTTTCCATATATTTTTATTATTATATTAATAAATTAATAAAAAATACATATTTAAAATAAATTAGTTATTTAAGTCGTTTTTGATTAAAATTAACAAATTAACTCAATATCGTTTTAAAATTAAAATAGTATGTGAATATTAGAAATAGTTATTTCTGTATTAATATGAATTGTTATTATTGTAGGAATTGCTTTTGCAATTCATAAAAAGTCATCTCGACACAATTATAATAAAACAATAAATTTAATCAAAAGAGATTCATCAAAACAACAAAAAGGCAACGATGGAGAATTTTTAGTAGCTTGAAAATTAACATCTTTGAATGAAGGCAAATATAAGGTTTTAAACGACATTCTATTGCAAATTGATAATGATAAAACAGCTCAAATAGATCATATTGTTGTTTCAACTTACGGTATTTTTGTAATTGAAACAAAGAATTGAAATGGATTTATTACAGGCGGTAGGGATTCATACTTTTGATTTAAAAGAAATTCGGGAAATAATATTGAAATAATGAAGAATCCAATAAAACAAAATGAATGACATATTGAAGCATTAAAGCAGATATTTTCAAAATACAATATAAATATAAATAAAGAATTATTTTATTCTCTTATTGTATTTGTCCAAGGCCAAAAACCCAAAATTGGTAATCTGAATAATTGCTCTGTAATACCTCTCAATGATTTAGTAAAAAGAATTCATTCATACAATAAAGAAATATTAGAAGAATCTCAAATTGAAAATCTTATAAAATTTATTCAACAAGAAAATATTGAAGATAAAGAAACAAGGTTAGAACACAACGAACGTGCAAAAAAAGTTTTACAAGTTCAACAAGATAAAATTAAAGAACATATTTGTCCAAAATGCAACTGCAAACTTATATATAAAAATGGTTTACACGGCAAGTTTTATGGTTGCTCTAATTATCCAAAATGTCATTTTTCAATAGAAAATATTGATTAAATTAATGACTTTTATAAGTAGCCTGATTATTTTTTAAATAAAAAATTACAAAAAAATATTTTTTTAACAATTTATTTAAAAATGGCTTATAATAATTACGCTATTAAATAAGAGCCACTGCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCATAAATGCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCATTTCAAGAAATTGATCAAGAGAGCATTGCTCTCTTTTTTATTATTTTTATTTATAAAAAATCAAGTTTATTTTTAAACCTGATTTTTGAATTATTCAACTATTGATTCTAAATATTGAAGTTTATTGTTTTGACTATCGAATTTTATTTTAGCTCCATAGGCAATAATAGTTTTTGGATAACAATGTCCGAAATTAACATTGTAAATTATTGGAATATCAAAAGGAACAACTTCTTTTCAAACTTCTTTATATTCATTATAATAAACGCGATTTTGAGGGCATCCGACTAATATTCCTTGAATATTTTTAAATAAACCAACTTCTTTGAATTTTAGCAACATCTTTTTAAGTAATTGCGGGTTTGGCTTTTCTTCACTTGTTTCAAGAAATAAAACCTTATCACATAGTTCATTAGGTTTCAATATTAAATTATATTTTTTAAATATTTCAGCTTCTTCTTTGTTTTTTTCTTCATCTAACCCACTATAAATAGTTTCAATGCAACCACCTCATAAATATCCCTCAAAAGATTTCTTTTCATTTAAAAATTCATATTTTGTAATGTTTCTATGTTTCTCTCTTGGTATATTCAATGCTTTTTTTGAAAAATCATTTCGTTCTTCATATCAATATGCACTTGGTTTAATTTCAAATTTAGTTTTATTATCAAATAATCATTTAAATGAGTTTTTAGTATAAGGCAACATTTCTTTGTCAAGTTCAGCAATATCACATAAAATGGTGTGCCCATAAAATGTTTTTAGTCCTAATCTATAAAACATTAAATGGTTAACGGTTGAATCAGAATATCCAATAAAAATTTTAGGATTATTTAAGACTAAATTTTTAAAGTTTTCGTCTTCTAATAAAAGTGGAATAGTTTTGTATGTGTCAAATCCACCAATTGCACAAATTATTGCTTTTACATTGACATTGCTAAAAACTTCATTTAAATCCTCAACTTTTAACATAGGATTTTCTTTTAAAACTTTTATTCCCTTAAGTGCGTTTTTAGCAATCATAACGTTAAGTTGAAAATCTAAATTTAGTCTTTTTATTGCTAAATCTAATTGGTGACTAGCAAATTCTTCTCCAATAGTTCCTGCAGATAAACTTACTATTGCAATGCCATTTTCCTTGCTTAATTTTATTGGTTTGATCATATAGAAATTATATTGAAAAGTTTATTAAATTAAAACAAAAAAATTAGACATGCAATTTTGTACAAGTCTAATTTTTAGGGCTAGATTTAATTATTTATTGTTTTGAAAATATTTGAATAATGATTCAATATCTGAAATAAATTTATTTTTATTGATAATTAATTCTTTTATGCCATCAGGAATAGATATATTGACCTCTTTATCAAGTTCTGCATTATCACTTGCCCCTAAAAAATAAAAGTCTTGGTTTTCTTCATCAACATAGCAAATTAAAAACGTGTAATTGTTATTTTTTTCTACGTCATCAACAAATAAAGCATTATCAGGTTTTTTATTGTCTAAATATTTTTTATAGCTAGTAACTATGTTTTTAGTTTTTTGTTGATTATAATCGTGTTTTGCGCTTTTAACTTCAATATATAAATAATTTGTTACTTCACCATTATTTATTTTAAAAACAAAATCAGGATATGAAGACTTAATTTCTCTTTCTTCATCAAAATATTCTAATTTTAAACCATGAAAAACAGGGTTTTTTGTATAAACTTTGACGTTTATTTCATTTTTATATTCACTAATTATTTTCTTTGCTTCGTTAATGAATAATGTTTCGGGATTTGAATCAAAATGATGAAGTCTATCGTTTAAATCTTTATATGCATAATTTTCTGTTTTGTTTAGTTTAACATTATTTTTTTCTATTTGATCAGGGTCAATAAATATTTCATTAGTTTCAGGCAATGTTTTATTGGCTAATATTTTATAAAGTTGGTTTGTTGAATCTATTTTTTCAATATATTGAGCATATTGTAATTTAAATGTATTGAAATATTCTTTTATTAAGATATATCAAAAAATATTTCTTGTTATTGGACTATTGTTTGAATAAATAGAATCATATTTTTCATTTAACGCTTCAATAAGAACTTCGTTGAATATTTTTTTATGCATAAGAAGAAATTTGTTGATAAATAATTCTAATTCAATTGAATTATTTATTCATGAATAAACTCTAATTTTATCTTCAATGTTTCCTTGTTCTCCTATTAATTTCTTGTTGTTTTTAATATATTCTTTATGGTATTTTTTAAAATATTCATCAATCTTTTCTTTAACAATAGAATTTTTAGCTTCTCTAATAAAATCTGCTCCTATTATTGCTTGTTTGATTTTTACTTTATTTATTTGGCCATAAACAAATTTTTCATTTTCAAATTCTCGTTTTAAAATCAATGTTCTTCTTATATTTTCTTTGGTTTGAGCATTTGAATAAATATAGTATCTATTGCCTTCTCTAGAAATTTGATCTTTGTTATAACTTGGATTTGGGAATCTTTTAATTCTTCCTATTGTTTGAATAGACAAACTACTTGATGACACTTTTCTAAGTTGCACAAGCATGCAAGCCCTCGGAATATTTCAACCAGTTGAAGGACCAATTTTAAAAATTATTGCATCAACATTAGAGTCATTTTTTGAAATTTCTTTTAAGTTTATTTTATGATTTTCTTCCTTTATCAAAGAAGAAGATTCCACTTTATCATTTGAAAAGTATTTAACATAACTCAAATTACATTCATTTAATTTTTTAATTATGTTCTCAATGCTTTCTTTAAAATCATCTTTTTCTTTGGCTGTTGATTTTTCACTTGGTTCATTTTCGACTTGAATAAGCATTGCTGGTCTTATATTTTTTAAATCACTATTATTGTTTGCATATTGGATTTGAACTTCTTTAAATGTTTGACATGCTTTATTTAGTAATTCATTATCATCAATTAAATCTTGACTTGAGTCAGCTATACCCTTGTTATAAATTAATTCATCTTTTAACAATTTAATATCATCTTCGGCTAAATCCTTTTCATCAATATAGACAAGTTTATGTGTTCCTTTAGGAGTTGCTGTCATTTTTATTATTAAATTGGCTGCTTTTTGAATTTTATATTCAAATCTAACTTCTTCTTTTTTTAATTTAGATTCATCATGGATTGTATCAATATCTTCAAATTTAAAATTACTTCTACCTTTTATATTTCCACCATGATGCGCTTCATCTCTAATATAAACTAATGTATAGTTTTGATATTTAATTTGATCAAGAAAGCTTTCAAGCAAACCTTGTTCGGTAAATATTCTATTTTTACCAAACGATGAAACACCGAAGATTATTACTTTATTTTTTTGAGGAATTAAATTATATGTTGAATCTTTATTTTTTTGAGAATTCAAGGAAGGAGATTCCTTTCTTTCTACTTCATACAAACCATTAATATATCTTTTATATTCATTTAGGTTTGATTCCATTTGATAAGGTAAATCTGCCGATGAAAGTGTAGCAATAACAAAGACTAATTTATTTGGTTCACCATTGGCATTATTTTCTTTTATTATTTCATTTATAGTGTTGGCCAACATAAAGGTTTTACCGCTACCAGTTGGAGCTTGAAAATCAACTATTTTAGGTTCGCTAGATTGAATTGCGATTTTATATTCTTCAAGCAATTGTTTAACTGCTTTTTCTTGTACACTAGTTAGATTCATTGCTATCTTTTCCTTCAATTGGTTTTAAAGCGGTTAAATTTAGTAATATTTCTTGTTCGTTAATTTTATTTACATTCGATAAGTTAAAATCATTCAACATGTTTTTAAAATCTTTGGCCGTGTAAAAAATATAAGGAATGAACAAAATTTAAAAAAGTAAGTAAAATGAGCTTACTTTTTTTCTTGCTCATGCTCTTTACAAACTATAAGATTTCTTTAATAAAGAAAAAACAAGGCACATGCCTTGAAACCTCAATTAAATTATACCATAATAAATTCTTTATTAAAAAATCTTTAAAAATTTTTTTAAGTAGGAGAGTTTATTGTGAGTAGGAATAAAGAAAATCGAGCCATATGAAGTCAATTAACAGGAGAAATTTATACTGAAAAAGACAAATGACTTGATTCAATTGAATATAAAGGAGATTCAAATTTTTGAAATTTATATAAAAATAAAATTTATTTAAAACTAAATGAAGATAAAATTAATGAAATATTTAAATGAAATATATTAATAAGTAATAAAATTTTTGAATTATTAAAAAAATCAAAATTAACTTTTTTAGTTTATCTAGTTATTATTAAATATTCAAGAAAGAAATGAATTAGTAAAAAATTTTTAATTGATTTTGGATTTAGTAGAACTTCAATCATCTTAGCCATTAATCAATTAAATAAATTAAATTTGATTTTAGAAAAAAATAATCAAATAAAAATCTCAAAAAAGTATTGATTCAAAAAAGGAGAGTCATATTTTAAACTACATGGAATCTTAGTTTGAAGATCTATTCTTCTTTTTCCGTTAGAATATTTAGAAAAAATAAATTTAATTGTATGAAAAAGTAAGGAAGTAGCAAAAAAGAGCTTTACCACAAATAAATCATCATCAATTAAAATTCAAAATAACTTTTTAAAAATGAAAAAATCTTGCGCTTATAAATTTATTAATTCTTTATTAAAAATATTAGGTCAAGAAAAATACGAAACTTTGTTTAAAGTTGAGCATAAATTTGACAAAGTTTCACAAAAATTTATAACAGAAAGAACATTATTGATAAGACTTAATAATAATTATGCATTTTAGGGTTTATGTAATAGAATAGAGGTTTGTTTAACAAATCGTTTAATTATAATTAAGTTTTAATAATTTAGATTTTATTATCTATTTTTGAAAATAGATCTTTTTGATATTGAAATTTTTGGCAAAAATTACTAAATTTCAATAAAAATTATTAATATTGACTCTTTTTTAAAATTAACTGTCAAAAATATGTCCCTAAGATAATTAAGGGTTATGAATAGAATATGAATAACCCTTAATTCAAAAGGGTGAAAGCAACCTTTAAGGGGGCTTGAACCCTAAGAATTTTGTTCCCTTTTTTAAAAGGGACACTTTATTCAAAGATAGAAAAATTTTAATAATAAATTTCTAAAAGTCTTAAAGCGAAGCAAAGATTTAATTTGAAACTCTTTCTAAGAGCGTTTAAAAGCCCTAGCTTGAAAGATTTTAAGAAAAGAATAAATTTATATTACAAATATTTTTTTCAGCAAGGATAATTAAATATTTCGTTCGGAGAAAAATTTCTTAATATATTTTTTAGAAAGTCGAAAATCTTGAATTCGAAGCAAAAATTAAATAATTTTATTTAAAAAGTCAAAAATCTTGAATTCGAAGCAAATTTTTAAAAGATTACTAAAAATTATTTCCGTAATCTTTCTGGTGCTAAAAAATTTCAATCAAGGAATTGTCTTTTTGAAATAGAACAATTAATTAATAGATTCTGTTAAATTAAATTCGGAGAAGAAATTTAAATCTTATTTTTTAATCAAAGCAAAAGTTTAATTGCTATTTATAAATTTGATTAAAAGAAAATTTAAATTTTAAATCCATTAATAATAAAAAAATTTTATAAATAATCGTTTTCATATCTTTCCTTTCATAAGCATATTTTAAAAAAAGTAGTGGTTTTTGAACTTTAAAAATTCTGTTTTATTAATTGTTAATTTTAGCACCAGAAAGATTACGGAAAATATAAGCCCAAATCTATTTTGTAGTAGAAAGCAATTGTTAAAAAATAAAGGAGGTAGATGGCCATAATGACTTTGTCAGAAATAAAAAAAGATTTAGATTATTGAAAAGAACACGAACCGCAAGACGTTTATACATTAAACGATGCCTGAGAATTAGCAGACCAAGAATTAATGAAATACGGAATGGAAGCAGATTTATGAGCGTTTGACATTAATAACATTTATGAAATGCAAAACGATTTTTATAATATTGAATTTGTGGGCATTGACGCCTATGGCAGAGCCTTCATCATGAGCAGAGAAGATGCCGAAAGATTTTTTCAAAATCAAGAAATAGAGGAAAACAAATCCTCTAAATCCCAATCAAAATCAAAAACTTTATAAGGAGATAAATGGCTATAATGACTTTATCAGAAATAAAAAAAGATTTAGATTATTGAAAAACTCATAGTCCTGAATGCGTGTTAGATTTTGATTCTGCATACGAATGGGCAAATCAAGAACTTAAAGAAAAAGGAATGGAGGCGGAGGGGTGGGCATTTGATATTGAAAAAATTTCAAATATAGAAACCTTTAATGTTGAATTTATTGCATTTAATGCTTATAATAGAGCACTTATAATTTCCAGAGAAGATGCACAAAAATATACAGATAACTCTGAAAGAGATAAAGATTATAATGAATAAAAATATGTCAATAGTTAGCAAAAGTTTAAGAAATTTTTTTAATTCTTTACCTGAAAAAAAGTTTAATTCAAAAAAAATCACCGAATATAAAGAAAATCATTTACAAGCGATTGAAGAATTTGTTCAAATATACCAAGAAAAAATACTGTCAGATTTTGAATATTGTGTATATGATGCTTGTGATGTTTTGGCTGGGAAATCATCATGATTTAATTTTGAAAAAGATTGGCCAAAGGAATTTTGCAAAGACTTTTTTGAATATTTTAAGGTATTAAATGAATTACTTGCTAATTGAAGCCCTAAATTTATCTCTGAGAAAATAGTAAAGCCGAACAAATCAATATTGCCTTTTATTATTGATGCATACAATATAAGATTATTGTTTTCTAACGAGGCGTTAATTAAGGAATATGCAAAGTACTATTTGAAACAAAACAAGTTAGTAGAAAGTGGAAAAAATGTCTAGAAAAAATTTTGAAGATCAGTATTTTAATTTCGTTTCAAGAGAAAATGGTGAAAATAGAGTAAATGGATATTTTGTTGATATATTAGATTCTATAAAAGACACTGATTATGAGCAAGATGCATACATTCAATATAGAAGATTTATAGATAAAATTGAAAATACACCAATTACTTACACCATTTATGATGCTTTTATTGAGGATTTGCAACAGAATAAACTATTAGAAAACAAATTTATCGATTATTATAGAAAGGAAATAGATACCGACATATATGATTGATACGATGTAAAAACCAAAGAAATTGATTTCGATAATTTATTAAAAACACTCATTAATAGGTATTCTTATTCTATCCCTCAAAAAGAATTTGAACTATTATTTGAAAAATCTTGAAACAATCAAGAGGGCTATCATGAAGGTTTTTTAATAGAACCTAATATCGGTTTTTATGATGCATTTCGTAATGACCTTGAATATATATTAGATGATTTAGATTATTCTCAGGAAGACAAAAAGCACTTTTTAAACAAATATATTGAAACTATTAATAATGAATGCGAAAGAATTATTAATTCTATTCGAAAGGAACAAAAACCAAATCTGCTTTTTATACATCCCTATGACAATGATAGAGGAATTTATTAGGTAAATAAACACTATTAAAACATAATAAAGAAAAGGATAAATATATGAAACATTTCAAAAAACTATTAATTACATTAACCGCTGCTGTTTCAACGACTGCGTTGCCATTAGTGGCAGCATCTTGCAACAATGAAAAATTAGCATTAGAAAATGGCAAAAAAGAATATGCAAATAACTTGCAATTATTAAAAAATGCTAAATCCCAATTGCAAAATTTGAATGTTGAATCAGAGCATCTAAAAAAAATTGATGAAAATATTGAAAAATCGATTAAATTGACTTCTAAAAATGCCACTTATAAAGAATATGCAAAAGCAAACACAATATTATCTGAAACCTTAAAACTGGCAACTCAATTAATTAACGATCATCCTAAACAAATTTATGAATATGCAAAGGGGATTCCTGAAATTCTTGAAAAATACGAATTGCATTTTTTAGAATTACCATTTTATTCAATGAAGCCAGAGCCATGAATTCAACCGCAAAATCCAGAATTTGACATTAAAAAAGCTTATGAATATTCAACTGGTACTCCTGAAATACACGGTAAAGAAAAAATGGACCTTAAACTTTTATTACCAAGCGCAATAAATGCAGAAAAGCAAGAATTAGAATATTTATCTGAACTTCTATTCAATTTTGCTATGGAAATTAACAATGCTAACGATAAAAATAATGAAAAATTAAATAATTTATGCAGTGTAATTTCACAAAAAGCAGGAGATATAAACGAATATCTAAACGAGCCCGGAACTGATTATTTCTTTTATTTAGAATTGAAAACTATTAAAGAATATACAAAAAATTGATTTTCACAAGTAAAAAATGAATGAAAAGAATTTAAAAAGCAAAATAATTAATTATTTTAAAGAGGGTGATTAAAATGTTATATAAATCTAAAAATAGAGTAAATTCTTGATTAAAAAAATGAAATCATAATTTGAAAAATCTAAAAAGCTGAGCGAAATTTTTTACAAAAAGCAAAAACCTAATACCCAAGGAAAATTGAATAAATTTAGAAAAAGAAATCAAAAATACCGAACAAAGAATATTTAATTTAAAGAAAATTCTAGAAGAAGCAGATTTCTTAGAAATCACAGATGAATTGGATTTAATTGAAAAAATGAATCAAAATATAAAGGAAGAAATTAAATATGGAATTTAATAAACTCCAAGCGGTAGGTTGAATTTCTAATATCTCAGCAATAAAAGAATCAAAAACAGGATTAAAATATAGAAATTTTGAATTTATCTTTTTTGAACCATCAAAAAACTCTTCTTCTAAATGACCAGCGCCTATTAAGGCTTGTTTATTTGAAGAAAATGATACAAATTTATTGCTTTTAAAAGAAAATCAGCTGGTTTCTTTGATAGGCAGAATTAATTCAATATTTTATAAAAGAAAATGTGAAATTTCAATTATAGTTAAGGAAATTAACAATATAGATATAGAAAATCAATAATCTTGTTTTAGTTTTTGTATTCGTTTATAAAGTATAATTTTATTAGTAAATATAATAAATAAAGGATTGTTTATGAAAAAAACTAGATTATTACTAACATTAACTATTATTCCTACAATAACAGCAATGCCGTTAATTTCAGCTAGTTGTGATTGATTGTATGATAATCCCAAACCTACTAAACCAAATAATTCATATCCAAATGATTTGCCAAAGCCAAAATTGCCAAAGGTTCAAGAAGTAAAACCAGAAGATAGATTCGATACAAAACTTTTATATTCTTGTCCTGTTCCAGCAGTTTCTGACAAAAATTGAGATGGTCCTCACCAACTTTGACATTATGTGGTTAATATTCATAAATTAAAAGTCCTTGATTCTAATGGAAAAGAAACAAATGAATATGAATCAAAAGAACAAAAATTACAAAGATATGCCCAAGCAAAAACTGTGATTTTAAATTATTCTAATGCAGTAGAAGAAGCACGCAATAAATTGCTAGGTAATTGATGGGATGATTTGTTTAAAAAATCAAAAGAAGAGTATGTTAGAAAAATTAAGAATAAAAAAGTATTACAAGATGACCCGAATTTTTTAATAAACTATGAAGGAAATTATCAATATTTTACCGAATCAAGAATTAAGGATAAAGATCCTCTATTATTATCCTTTTCAAATGAAACAATAAAAGAAATTAGAGAGCAAAACATTCAGACAGCAACTATTTCATTGGGACTTTCTCAATATATTGAATCATTTATGGAAATAAGAACAATTAGCGATTCTTTATTTAAAATAATTGACTTATTTACGTTTATGATAAACAATATAAATGACCCTGACAAAACCTTCTTGGAGCAACTACAAGCAATGGGTCCAGAGAAATTAGATAAATTAAAACATCTGATAAAAAATAATTTAACAATTGGCCAATTAATAAGCAATATAAAGATAGCCTTAGAATTAGACAATAAACTTTATGTATTTCCTGCTCCTATTGTCAAAAATTTTTATGATAAAGCAGGTCATTACAATAAAATCATTGATGCTTTAAATGAATTTTATAATCCCTTAGCATTTTTACTAGGTTTTATTACTGAAACGAATGATTTTTTTTGAAATAATCCTAATTATTTATTAAAAAATAAAGCCAGTTGATATGACAAATATTTATTTAATAAATATGAATATGGTTCAAATCCTAATGGCTTTAAAATAAAAACATTAAAACACTATAATGCTCAATCTGCATTTAATGCATTAAGCAAATGACTAGATAGTAATAAAACATCATTAGGTTTAAAATATAGTTCTATAAAATAAAAATTAAGAAATCTTATAGTTTGTAAAGAGTATGAACAACACAAAAAAAGTAAGCACTATCAATATAGTGTGTTTTTACTTTATCTATCTTTACAAAAATTAAATTTTATAAGGAGAATTATGAATAGTGGCACCTCTGGTGTAGAAACTCTTAAACAAGCATTAGGAACGTTGTTTGAGCAAATAAATCCATTTGTTTCACTGATAGCAGGTTATGCTGTTGTTATCTTTGCCTTAATTGCTATTGCTATGACATTCTTTGCGATTGCTTCATTGAAAACAGCCAAAACTCAAACTTCTAAGAAAGAAGCTAAATCTAGAATAGTGGGGATATGACTTTCATTTGGTTTCCTAATTTTCTTTGTTGTTTTAATTCCGGTTATTTTAAAAGTAGCCACCACATTTTTAGCAAATTAATTTAAAGGAGATATATGTTTCACTGAACATTTTTAGTAGCAATTGCCTTGTCGCCAGTAGTTTTAGCAATGTTTATTTACCTTGTTGTTCTTTTTGCTCAGAAAACAAAAATAAAAATTCAATATGACAATAAATTAGAATATAAATCGGATGCAAAATACAAAAAAATAGATAAATTAACAAAGATATTTATATCAGTTTTTGCAATCTTGATAATTGCTGCATTAGTATTAATAACTGTTGGTATTGGTAATACTTATTTTATTAAATAGGAATAAATAGTGCTAATTTCAGCAATAACCATATTATTATCCCCAATTTTATATGCAATTTTTGCATTATTTTGGGGAATTTTTATTAGTTTTCCATTCCTTATATTGCAAGGTTTGTTCAAGGTAGCAAGATTTTTTGGTTTTGAATTAATTAACTATATTATTTTCAAAGTTAATCCAGGAGAATATACATTTAGTTTTTCAGATTTACCAGCGGCGTTTTTAGCGTTTTTACTATATGGATTAATTTTTACTATTATTTTTATAATTATTTCATTTGCTAAATATCAAATTGGCAAGAAAAACAAGAATCAAAACATAGAACATTTAAAATTCAAGAATGTTCTAAAACAATCATTTTCGGCTTTTCTATGAGCACTGTTATTGCCTTTTTTAATATTTGTAAGCAATATAGTTTTAGCAATAATTTTTTACTATTTTGGTCAAGCTCTCGAACTTCAAACCAATATGAAAGGTGATATCATATCCCTTTTATACGTAGCATTAAATGATGGTGCTAATTGAGCAAAAGATTCTGTTGCAATGAATAATTTTGCTCCAATGCCTTTTAGTGATTATGTAGCCCAGTTGGTTTCAAATTCTTGGGGATTAGGGTTAATGTTTATTAAAAATACAATAATTTCCTGAGTTTGTGTAATTATGCTAGGGTCTTTATTAGTTAATATTGTTAAAAATTGTTTAAATCAATTTACTTTATTTATGATTTCACCATTAGTGGCAGCAAAATCAGTTGAAGATAATGGCAAAGCAATAATGAATTGAAAGCACAAATTTGTAGAAAGCGTTATAAGTATTTTTATTTCATTATTTGCTTTAAATTTCTTTGCCATATTTATTCTAATCTTGCTTCCGATAAAAGAAAAATTGAGGAACTTAGGAATCTTAATAATACCTGACCTAGTTATCTATATTGGGGCAGCATATGCTACAAAAGCCCTTAATAAGTTAATTGGCGATTTGCTAGATATTCAAGTACCTGACTTAGGATTCAAACAAGCCGGTAGATTTATAAGAACAGGCGCAGGAATTATTGCTGGCGCTACAACTGCCGGAGCAGCCGTGGCAGCAGGCAATGCAACATTAGGTTCAACTATTTCAAAGGGCGGTTTTTCAAACGCAATTGGAGGCTCTATCGGTAAGGCGGTTAATATATCGAGAATTAGACGAAATAAGGCATATGCAAAAGACTTGGGAATGAACAAAGAATTTATTAAATCTTCGTTTGAAAACGTGGGCAAAAATAAAGTAATAGAACAAAGAAAAATGCTTAATAATTTTAAAAATATGGCCACTGATGAAAAAGGAAACCTACACAGAGAATCATTATATGATTTGGACTCTTTGAAGCAATATAAGGCATCAGAATTACAAAAATTCAAAGATAAACAAGAATTATATGATGAATCATATAAGTTTTATAAGAAAACAAATACAGACAGTTATTTAGAACAAAAGCGGGCTGAATTAGAACATAACAGAACTCAAAATAAATATAAATACTCTATTTCTAAAGCAGAAAATCAATTAAAGCAATTAAATGATTTTGATTTAGAAAAAAACGATGTAAGAAAGGAAAATGAAAAAGATGCTTCAAAATAAAAAATTAGCAGATAAAAAATTAACAATATGAAGAAAATATACAGTTTTTGATTTTGTAATATTTTTTGCAATAATAGCATTAGCAATTGTTTTAAGCGGTTTTACTAATAAATTCAATTGAAAATTTAAAATAGCAATATTCATAACATTTATTCTTTCAACATTTTGAATATTGTTATATTCAAAAAAACATAATTGTAGATTTTATGTCCTGGGTTTTAGAGCCTTTAAATATCTTGTGGGAGTAAGAAAATATGATATTAAATCAAAAAATAATACTAGTTTTTTAATACCATACAGTGAAATAATTAATGATGAAATTGTAATTAATAGAAAGAATAATTTTGGAATATTTAACCATTTCAGTGTTGTTAAGATAAAAGGATTTAATGTTTTTGTAAATTCGGAGCAAGATAGAGAAGCGGAGTTTTTGAGATTTACAAATCTCTTAAATTCTGTTGATGATAAAATAACTATTATTAAAACCTCTGCACCAATGGATTTAACTTCTAATATTAATGAAATTGATAAAAATTTAAATACCTCTTTTATTCAAAATAATAATCTAGCAAAAGAATATTGTTTATTGAATAAACAAGAATTAGAAACATTAAACGAAAAAGACAGCATTGATAATTATTATTTAGTTATTTATGGAAATTCTATTGAAGATTTAATGGCTAAAAGAACGAGAATAATTTTGCAATTAACGAATACAAAATTTAATCCTCAATTAGTAAAAGGAATTGATTTACTAAATGTATTAAATTTAATATTTAGAGTGCAATTAAATGACAAAAATTTATCCAATATTGAAAGGCCAGCATCATTACTTGCTCCTAAAAATGTAGAATTTTTTAAAAAGTATATGGTAGCTGATAATAAATATATTAGTTTTCAATCAATAAATGATTTCAATAACATTGAATTACAAGATGCTTGAACTTATAAACTATTTTCTTCAAATTCAACTGTCATTTGAAATCTAAGTGCATTTGATGATGATTTGAAAGAAAAGATATTAAACAAAGCATCAGCAATGGTGGAAATAAACAACAATAGCAAATCAAAATTTAAAACAGTTAAATCAAAGGTTGAAATGGAAGCAATTGAAAATCTTATTTATTTGGCAAATACAAAAAATATGAATATTTTTAATAGCAATTTTATTTTTGTAAATCAGGGCAATAATTTAGACGAAATTAATGCATTAGAAACCCAAAATCAATTAAACGCTTCTGAAATATCAGCAAACATAAACCCTTTATTATTTAAACAAAAACAAGCATATGCAAGCGCTTGTTTAATAAATGGTGATAATATTGGGGATGATTTAGAAATGGTTTCAGAAAATATTTCAAGAGGTTGAGGATTTGTCAATTCTGAATTAAACGATAAAACCCCATTACTATTTGGTTTTGATATGACAACTGATTCACCTTTATTTTTGGATATAAAAACAAAAAATTCGCAAAGAGTTTATTCGAATATGCTAATTACTGGAACACCAGGTTCAGGCAAATCGACACTTATGAAAAAAATGGTATTGCATTATTTAATTTATGGCACTGAAATCATTATTTTAGACCCTCAAAGGGAATATACAAAATTTGCAAAATTATTTGATGGCGAAGTTTTTGAAATGGGAACAGGAATTAACACGTTAATTAATCCTCTTCAACTAGATTTGCAATTTAATGAACAAGTAGAAATTAAAAATCAAAATAAAATTATAATATCTTCAAATATTAGAAAAGTTCAAAAATTCCTTAAAATAATGTGTAATTTTAATGATATAGAAGTTAGATTCATCGGAAAATCCTTGTCAGAGTTATATAATAAATGAGGATTTTATGATGAAAATATCAATTTTTTAGAATTTCAAAACACTGATTATCCGACAATTAATGACTATTTAGCATTCTTAGAAACATATGATTTTGATAATACAAACGATAAAGAAATATACCTTCAATCTCAAAAATTTGTTTTAGCGACAATAAAATCAGAATTTACAAATAATTCACCTTATGAAGCATTATATAATGGCTATACAAATATTAATTTTTCTTCAAAATTCAATATTATAGATACTTATAATCTTTCGACAAAAGATGATTCGCCTGCTTCTAAGGCTGGATTATTCTTGATATTATCTTTTATTCAAAACAAAATTTCTAATAATTACTTTTCAAATCCAGAAGGGAAAAATTGAATTATGTTAGTAATTGATGAAGCGCACAAATTTATTGACCCAATAAATCCGATAGCATTAGATTTTGTTTGAGATACAGCTAAAACAATAAGAAAATATCGAGGAATGTTAGTGTTGGGAACGCAAAATTTTATGGATTTTAATCAAAATCAATTAGTTCAAACTAAATCTGCGGGGATTCTAGAAAATATGCAATATTCAATAACTCTAAGATCTAATTATCAAGATATAGAAGCATTAAAAACTCTATTCGAAAATAGAATGCCTTTAACAGAACAAGAATTGCAATTTTTATCTTCTGCTCCGATTGGGGGTGGAATATTATCAATCGATAACACAACAAGATTTCAATTCGAATCTTATTACAATGAATTAGAAAGAAAATTATTATTTAACAATGGCGATTTAAAAGCAATTGCAAATAATTAAGGAGAAATAATGAAAAAATGAAAACCAATTTTATTAGGTACAGTTTCAATACTTGCGCTTGCCGGAGTAGGTCTAGGCATTAAAGCGTGTCAAGATAAAAAAAATAAGAATAAAAATTTGACCAATAACAATTTTATTCAGTCAAGGGCTTCGGGTTTTCTTCACAATTATTGAACTGAAAATATACCCGGCGCAGATTTCCAAGATAAAGTTTTAAATACTACGGTAAATATCAATATTACTAATCCAGACGAAAAGACCCCAGAATGAAAAGTTGGAAACGTGAAACTGGCACATACAATGCCGGCCCCATGAGCAACAGATAATGAAATAAAAAAAGCCATGCTAAATAATTCAAAAATCAAGGCACAAGTTCAAGAACAAATAGAATCACAAATAGGCGCTTATTTGCAACGTTATTACGGCACATATAACTACAACTGAGATAATAGATATTTGCCTTATAAATGATCCTCATCTTCAATCAATTTTGATACTTTTTGAAAAAATTATGTTAATGACATAAATTTAAAAAATAATGTTGAATTTGGAATTAAATGAGAAGTTGTTATAAGAACAGGGCACGGAAGAGCATCAGCCGATAAATTTCATCAATACATTAAGTCTATAAAAAATGATAAAGAAATAACAATACAAATTAAATTGAGGCCTAAGTCACGCCAAAAAGCATTTGAATACTTTAAAAAAGAACTTTCTAAATTTATAGAAAGAGGAAAAAATGTTCAATTAATTACAGATTATAATCATGACAAAATTGATGATTATAAATTATTAGAGCCTGAATATGAGCAATTTAAAACTAACAGGCAATATTTTAAAGAGTGGGCAAAAAAACTAGATTTATCATTTTCTTATGAAAATTTCACTTGAAAAATTTCTTATAAGCTTGATGAATCCAAAAAATTTATCAACATTTATATGGTTAATACATCAAATGATGAAAAATTTATATTGATTGAAAATCAGGGTTTCGATTTTAAAAAATCTAATAAATTAGAAAAATTAGGAATAATAAATAAATTAAGGTTATCAGAAACTCAAAAATATTTAGCATTCGATCAAATAAATAACAATAATCCTACCGGAATGGCAAATGATAAGCCCGAAAGAATAGCAAGCGATAAAAAGATAGGTCTTGAAGGACAAAATTATTACCTGGGAACTTATAAATTACATGCTCCTGCTGTCTTTTCTTTTACGGCGCCTAATGACAATTATGTTGTTAAGGTAAATGGCCATAAAGTTGATTTAATTAATAACAAATTCAATATTAAACTTGCAGATAAAAGAGAGGATGCAAGCGATGAACAAAAAATATACAATCAAGATTCTGATTCTTCTAAACCACAAGATGAAAAGAATTCGCATAAAAAAAATGAATATACAATTACTGTTGAAGAATATAAAAATATCAATGATCCATCAACAAAGACTATAACATATACCAAAAAATACATTATTGATTCAAAAAGTGGAAGTTTAGATTTTAAATGATATGCATGAGACCCTAAATATAATAAGGACCAAAGAGAACTAATTGAAGAATATTTAAAAGATGAAAAAGGAAATATACAAAAGGACAAGGATGGTAATCCATTGCCTAATCCTAAATATGACCCGTTTATTGACAAAAAGACAGGCACAAAAAAAGAATTAGTTTGATTTGACACAACAGATTGAAGTTCTAATTATCAAGCATATAATTTAGGCGCTCTAAACCTTTCAAATAATAGTTTTGAATATATATATGCAAGAACAACCGGATTGCCATTCTTTGCATACACATTGTTTAATTCAAAAGGCGATAAAATACGAGATAAGGGCTTTATTGCAGAGGCTACCGTAATCTCAAAAGCTGCTCTAAAAATGCTTGCCGGTAAAACAGAAGAGTATTACATAATTAAAATTGATAATAAATCAAAAAAATGAGAGAATCCTGAAAAATTAGATAATTTTGTTTCAGGTGATAGCTATTTGTCAAATGAAGGAACATATCTCTTATATTCTAGAAGTTCAAAAGATTCAATTAATAACTTCAAGATTTTAAAAATTAGCAATGAACAAACAATAAATAAGAAAATGTTTTCAGAAATAAACAAAATTGATTCAATTAAGAATTTATGAAAAACTACAACCGGTTATTTCTTCGCCAAGTTTTTAAACGAAAAACACAACATATCGTTGGATGAATTAGATAAACTAAAATATGAAGAAGTACAAATCTATTGAAATTTATATATTACGTATTTAGCAAATAAAAATCAAGAAAACATTTATATTACTCCTAGATTGGATTTTTCATCAGAATCTAATAAATTCAAGGATAAAACTGAATTTTTAGAATATTTAAAACAAAATAATATCGATATTATTGATAAATATGGAAAATTTGAAAATAAAGAATTAGTAGGAATTGAAAAATATAATTTCATAGACAACAATCAACTAGAAATTACTTATAATCTAAAAACTGCTGATGACAGGTATAGATTAACGGTCTTAAAGCAAACTTATTTAATTAATTTTATTAATGATAAAACAAATAAAACCGAAATATTTATTCAATGAAATAAAGAATTAATCAAAGAAAAGATTAGGAATAATTCAGAATATAGATTTTTTAAATGGCTTTTTTCATCATTTACTTCGGCTATAACTAATAAAGAATCAGAAAAGCAAGCATTAAATTTTAATTATGCATATTTTGACAATAAATGATTAACTATATATGCGGAATTGAAAAAAGAATATGCTGATAAATATTATTTGACTAATAACCAATTCAAAATTGAGGCAATTTTTAAACCAGTTGATTTATTTGATGATAGATTTGAACCTAAAATGATTAATTTAAAAGGTGAAACAAGAGAAGAACAAATAAAACAAATAATTACTCTTGAAATAAAGAAACAAATTATTCAATTTTTTAAGAAATTTGACATTGATGTATCTGAAAACGACATAAAAATTCAAGAAATTACAAGTGAAATTGTACAAAAATTGAAAAATGTTTATAAAACAAAAGAAATTGCGTTAAACAATGCAACAAAACTAAATGTTGATATAAACAATAAAACACATAATATAAAAGCAAAAACCACATTTGAAATTATTAATTTTTCAGATACTTATGTTTCGAATATTATTGATTTAAGCAAATTAAAGCTTGACCCTATTGATATAAATTATGATGTTTCTTTTTCAAATAATAAAGAATATCAAACAAAATGAAATGCTATGGCAAATTTAGTTAAAATAGAAATTCAAAAGCAATTAAATTCCTTAAATCCTGAATTAGTTTTGACTAAAAATGTAATTTATAATGAAGAGGAATTTGAAAACGTAATTACTAATTTATTTTATAAAGACATTATTTCAACATTGAATATATTTCCTAACCATCCAATGATAAAAAATTCTACTTCAATAGAAATTACTAATGGCCATAAAACAACACAAAAATTTAATTTTGGTGATTTAGAACTAGATGATTTAAACATTAAATCAAAAAATTACACCGAAATTAAGAAATTGATAATTCAATGAGTAGATAGTCAAATTAGATCTAAATTGTATATAACCAATTTAGCATACGAAAAAGATTATTTAATCGAAGAACTTAACAACATAGATTACATGAAGACCTTAATATCTAAAAAAGGTAAGAATGAACTAACATTGACCATAAAACCAGTTAATAAAAGTTATGAAGGCGAAACAAAATTAAAAGTTATTAATGATTTTGATGGAAATTTATCGGAAGAAGATAAAAAAACCTGAGAAAAACCAGATGAAAACATTTATATTACTCCTAGATTGGATTTTTCATCAGAATCTAATAAATTTAAGGATAAAACCGAATTTTTAGAATATCTAAAACAAAATAATATCGATATTATTGATAAATATGGAAAATTTGAAAATAAAGAATTAGTAGGAATTGAAAAATATAATTTCATAGACAACAATCAACTAGAAATTACTTATAATCTAAAAACTGCTGATGACAGATATAGATTAACAGTCTTAAAGCAAACTTATTTAATTAATTTTATTAATGATAAAACAAATAAAACCGAAATATTTCTTCAATGAAATAAAGAATTAATCAAAGAAAAGATTAGGAATAATTCAGAATATAGATTTTTTAAATGGCTTTTTTCATCATTTACTTCGGCTATAACTAATAAAGAATCAGAAAAGCAAGTATTAAATTTTAATTATGCATATTTTGACAATAAATGATTAACTATATATGCGGAATTAAAAAAAGAATATGCTGATAAATATTATTTGACTAATAACCAATTCAAAATTGAGGCAATTTTTAAACCAGTTGATTTATTTGATGATAGATTTGAACCTAAAATGATTAATTTAAAAGGTGAAACAAGAGAAGAACAAATAAAACAAATAATTACTCTTGAAATAAAGAAACAAATTATTCAATTTTTTAAGAAATTTGACATTGACATATCGGAAAATGACATAAAAATTCAAGAAATTACAAGTGAAATTGTACAAAAATTGAAAAATGTTTATAAAACAAAAGAAATTGCGTTAAACAATGCAACAAAACTAAATGTTGATATAAATAATAAGAAACATAATATAAAAGCAAAAACCACATTTGAAATTATTAATTTCTCAGATACTTATGTTTCGAATATTATTGATTTAAGCAAATTAAAGCTTGACCCTATTGATATAAATTATGATGTTTCTTTTTCAAATGATAAAGAATATCAAACAAAATGAAGTGCTATGGCAAATTTAGTTAAAATAGAAATTCAAAAGCAATTAAATTCCTTAAATCCTGAATTAGTTTTGACTAAAAATGTAATTTATAATGAACAAGAATTTGAAAACATAATTACTAATTTATTTTATAAAGACATTATTTCAACATTGAATATATTTTCTAACCATCCAATGATAAAAAATTCTACTTCAATAGAAATTACTAATGGCCATAAAACAGCACAAAAATTTAATTTTGGTGATTTAGAACTAGATGATTTAAACATTAAATCAAAAAATTACACAGAAATTAAAAAATTAATAATTCAATGAGTAGATAGTCAAATTAGATCTAAATTGTATATAACCAATTTAGCATACGAAAAAGATTATTTAATCGAAGAACTTAACAACATAGATTACATGAAGACCTTAATATCTAAAAAAGGTAAGAATGAACTAACATTGACCATAAAACCTTCTAATGATAGTTGCGAAGGTGAAACAAAATTAAAAGTTATTAATGATTTTGATGGCGACTTATCTAAAACAGAAGATAAACGCTGAAAGCAAAAACCAAAATTAAATGCATTATCAATAGGATTAATAACAACTTTTTCATTACTTGGTTTTATAGGTTTAATAATACCGTTAATAATATTGATATATAGAAAAAAATCTGCAAAAATTAAATAGATAAGATTTTTTAACTACTATAAGGTAGTTTTTTTATATTTGAAATGAAATTAATATATGGAAGCGATAGAAATAATTAAAGAAAAAATAATTAATGAAGATATAGAATTCTTTAACTCGGTAGCTAGAAAAAATCAGGGTTGAAGAGTAAAAGATTGAATTTATAGAAAATTAACATTTGAATTTGGAACGATCAGAGTCAAAATTAGAAAATATGTAAGAAAAATAGATAACAAATATGAAAATTATAATGTTTGCTTATCTTTGTTTAAAGATATTAAAAATAAGAATATTGGCTTTGAATTACGTAAAAAAGTAATCTCATTATATTTAAACGGGCTAAGTTATAGAAAAATAGCTAAAAATTATGGAATTAGCCACACAGAAATTTTTAAAATAGTCAAAAATCATTTTCCTCATTCTTTAAAAGAAGCAAAAATAATTAATTTTAAGGAATTTATAAACAAAAATGAGAATATTTATATTGCAGTAGATGATACTTATTTAAAAGTACGACAAGGCCAAAATGAAATAAACAAAATTAAAGCAAGAATAATTAATTTCTTTGCATTAGATAAAAATAAAAAGCCTTATTGCAAAAACCATTTGATTTATTTTTCGAGCCCAAATAGAAATATGAATTTAAATGATTTAGTAGAAAAAATTAATTCTGTAATTAGGAATAATTATTCAGAAGAAGCCAATATTATAATCACAGGAGATGGGGCAAAATGAATTCGAACACTTTCTAAAAAATTAAGTGCAAGATATATTCTATGTAAATTTCATTTACATTCTAAATTTCAAGTTATTTTTAACCATTCAAATCATTTAAAAAAAGAGCTCGAAAAATTAGAAAAAATCACAAAATTTAACTTGTTTAATATTTTATACACATATTTAAAAAATCACAAATATATCGAAATTTTTAACCTAGTGTCTAAACATTGAGATTCTATAAAAAAATGTTTAAGTGAAAAAAGAGCAAAACTATTACACGATTTTGCTTATTATGTACATTTAAACATTACTGGTTTATCAGAAATTGAAGAATGCGACAAAAATTATTATGGAAACATTGCCGAATCTTATGTTTCACATATAATAAAATCTAAAACTAAAAAATTTTATTCTATTTGTAATATAAAAACAATTATTCAAAGAATAATCAATCCTTATGATTATATAAAAAACAATATTGAAATTCATATTATTGATTAGCAAAATTCAGATTAATTACTAATAAACAAAAAAATAGGCGGGTTCCGCCATTTTTGCTTTATGCAGCAATTTTTTTTAAGATCTAATTACCTAATAAAAGAAATTTTTTTCTTATTTTTTTAGATTAATATGATAAGATATTTTTGTAAAGTAGTATTACTTTTTTAAAACATGTTAAATTCCTTTAATAATAAACACGACCAAATCTTTTTTAATTATTTCATTGTTGTTGGCAAGTACATCAGTGTCACAATAATTTATTTCAAAGACATCAAGGTTTTGATTATATGGTTCGTTTTTCTTTATTCAATCAAAATCAACTTCGCCTTTGCTTCCCTTGCCATTATTGATTCTATAAAGTCTTTCATAACAAACATCAATACCAATGTTGTTTTCATTGTTTGTAACTAATGTGAATGTTCTATTGCCACTATCTTCTTTATTTAAATCTAAAACTGCATGACCTGTTGTTCCTGAACCTGCAAAGAAATCTAATACACGAGCATTGGGATTAGGGTGAATGTTAATTAAATACTTAATTAGTTCGATTGGTTTGGGGAATGAAAAATCTTTGTTATTTAATATCGATATAAGCAGTCTCTTGCCAGTACTTGTGGATATATTTCCTTCATTAATTAAATTAGAAAAATTGTTGCCTCTTTCTCTAGAAATGATTTTGTATGGTTTATTTCTATCAACTATGACTTTTTCATAAACTTTCCGATATGCTTTTCAATATTTTGTTCCGTCTTTATAGGTTTTTTGTTGAATTTCAATAAATCTATTTGAATTATAAAATTCAAACAATTCCTTACCCAGAGTATAACGGTAAGAACGAGGTTTTAATATATTTCTATAATTTTTAAACATTGTACCATCTGGAGCAAGAATTTCATAATCAAGTGATTCGATATATTGAAAACTGCTTTTGGATGAAACATGGTCCAAGTCAGTTAAATAATATTTTCCTCTTGTTTTTACAAATTCGTCTTCTAAAGTAAAATTCTCAATATCTATTTTTTGACTGTTTCAATTGCTTTTTTCTTTGTTTTTGCTATAACATACAATAAATTCTGTTTCATTATCAATGTTTGATTTATCAGATCCCCCACCTGTATTCTTTTTTCTTCACACAAAATTACATACAAAATTTTCTTCCCCAAAAATTTCGTCCATTAATACTTTTAAATAAGCTTGTTCAGTGTCATCAATTGAAACAAAAATAACACCACCTTCTTTTAAAAGTTGGCGAGCCATTTTTAATCTTTCGTTCATCATATTTAGTCAACCTGTACGACTAAATTTGTCTCTATAAATAAATTTAGACGCTGCAACATCATCTTTTTCAGATAAGTTATTTCCGTCATCTTTTGCACTTTCAGTATTATAAGGAGGATCAATATATATTAAATCGTAATTATAATCCCCCCCCCCCTATTTGTTCTTTTTGTCTTTCTATTACAAGTAGGTTTTTTAATGCATCATAGTTTTCACCAATAATTAAAGTGTTTTTATTAGTATTCAATAAATCATTTGTAAATGATAATTTTTCTTGTTTTTTTAATATTGCAATTTGTTTTGCATCAACACTAGGCGCAACATCAAAAACAAAGCCTGTTTTAACTCTTTGAATCAAAAGTTGGAAAACATTGTCCAAGTTTTCTTTATTTGAATTGTTTGTGTTAACTAAAATTTCTTTTATTAATTTCTTTTGGTCTTGATTTAATAAAAAAGAATTCAAGTTATCTATATCATCTATATAGTTTTTTAAATCCTTCATGAGTTGTTCTCCTTGATATACCTAAATTTAAGATTTATATAATAATTTTAAATTATTTGATTATTATTTCTATGATTTCCGGCTTATTTCTTTACTATTTTTTAGTTAAAAAATTAAATTTAATAATTTAATTAACATATTTAACATATTTTTTTAACCTTAAATTTAAATATATTTGTAAAAAAATAAAAAAATTTGAACTACATTTTAATAAAAGAGTATTATTAATAAAAAGAAACGCAAACATGCGCATATATTTCGATATATGGCATTTTTATTTTATTGCTAAAAAGAAAGGGGGATAATTATGAAAAGCAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGCCAAACACCAATAAAAAAAGCAATTTGAATTGTTGCTATTCCAAGTTTGCTTGCTGCAATCATGGCTGGGCTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTGCATTCAATTACGAAATTAGCTGAAATTACTCCTAATAGTATTGTATCGACAGCTTCGGCAGTATTTGGTCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTGCCTGTTGGAGCTTCAATTTATTACACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACGATGTTTTGGTTTACGGTTGCACTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGAAGTGGATTACTAAATGTATTAGCAGGTGAAACTCATATTGATTATAATATTGCTAGAATAGCCGGCATAGATCCTAATGCCTTGCAAGATTATTACAGCGCTGCATATTCATTAAGCATAAAATGAGCAAAGCAATTTCTATATATTTATGCATCGGGAACAATATTACAAGGATTAACCCTTTATCTATCTTATTTTATAAGAGCAGAAGGCTATAACAGTTATGTAATGATTTGTGCTATAGTTGCAAATGTAATAAATGTTGCTTGTGATGCGCTATTTATTATTTGCTTTAAAATGGGAGTTTTGGGTGGTGTTATGGCAACCATTGTTGGTTGAACATTTAACACAATTACTTATTTTGCTTATATTTCAATAAAGATTAAACAAAATAAATCCTGGTTATCTCTTTCTCACCTATTCAAATTTAAATTTAAAAAAGAATTATTGGGACCAACATTCTTACTTGGTGCAAGTGGATTTTTAAGAATTTTTGGAATAAGTTTTTCATTTTTAATTATGAATATTCTAATTACAAAAACTTCATTTTCAATGCCTGATTATTTCCAATATTATTGAGCAAAAACCCAACCAATTATTATGTTATTCTTGACATCAATATTTGGCATAAATGATGGTGCTAGAAGCTTATTTACATATAATTATTCACTAAGAAACATGGATAGATGTAAACAAGTATATAAATGAACTATATTGATAGCTATTCTATATTCAGTTCTTGTTTATATATTTATTGCCTTAACATCTAATAATTTGTGAATATGAGTTTTAAACATAGACCCTGATAGAATTGCAGGAACTAGTGTATTTATTAAGGTTATGTCGCTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGTTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTCGCCTCAATATTTGAAAACTTTATTTGCTTTGTAATAATTGTTCCTATTGGATATGGAATAGCTTGAGGAATATTTAATCATGATTCTGCTAACAGAGTAATTTCAAATTGAATCATTATTGCTGTGTTTGTTTTCAACTGTGTATTTGCTTCATCAATATTGATGTTTTATTCATGATGATTTGTCTATAAGAAAATGCCTTATATCGATCAAGCAAAAGTTAGTTGAAGTAGAAGAATTGAACATAAGTTTTTTGAACACGCAGAAAAACAAGAAGAACTATACAATGCTCAAATACAACAAGAACAAGTTAATGAAAGTAATAATTTAAAGCAATAAAAACTAATAAGAATTTATATAAAAAAACTTGCAAATTCGCAAGTTTTTTTATTTCTTATCGTTAAACAATTGCTTTAGTTTTATTGTCTTAAATTTTGTTGTTGGGCTGGGTGTGAATTGTTTTTACTATTATAAGTTTTTACTGCAAAACATAATAATGCAATTAAAACACCTAATTGTGCAAGATTAGCAATTACTGAAAGTCCATATATTGCTATAGCAGTTTTAGCAGCGCCTGATAAAAACATTGCTGTAGCAACAATTGCTAAGACAAATGAAATTATAAACAAGGCAACTAATGAAATTGATAATTTTCTTGTTGTTATTTCATTTTTACTTAAACCGCGAGATCCTGTTTTAAATCCATAAACAACTGTTAGAATAAAAAAGACGATGCTTGCTATTGCTAATAATAATCCAAATACTGGTACAAATGAACCGCAAATAATATAAATAACATCTATTCCTAATAAGATTGCTGAAAATATTATTAGAAGTTTTGCATTTTCTTTGGTTTTCAAGTTTTTTGAATTATTTTCCATTACTTATTTTCCTTTCATTTTATATAAAGCATAATAATTATATAAAATTCATAATTTTTAAATATTTTAAAAATAAAACATCAAGCGCTTCAAAACTAAATTTTGTTTTAAATTCGTGTTGCTACTTGATGTTTTTTAAGTAATTTTGTAATTTTGAGCTTTTATTGTTGATTGTCAGAATTTCAATTTTGAGGCTTTTGAGAGTCGCCACCATAGTTGTTTGTTTCATTATTAACATTATTGTTATTTTTAGCACAATAGACCTTAACTGCAAAACATAATAATGCAATTAAAACGCCTAATTCTGCAAGGGACAAAATTAAACCAATAATATATAGTGCAGATGTTGATGACACTAATAAAAATGGATTATATTCATTTGGGTCTTCATAAGTATATGCGCCAAGTGATAAAGATATTGCTATTATTATTGCAACAATAACTATTGCAATTGCTGCTGCAAACAATGCTATTAATGCAATGGATAATTTTCTTATTATCATTCGATTACTATTTTTTGGTTCACGTGATCCTGTTTTAAATCCATACACAACTGTTAAAATAAAAAACACAATGCTTGCTATTGCTAATAATAATCCAAATACTGGTACAAGCGAACCACAAATAATATAAATAACATCTATTCCTAATAAGATTGATGAAAATATTATTAGAAGTTTTGCATTTTCTTTAGTTTTTAAGTTATTATTCACTTCATTCTCCTATTTTATAGCTTAGATATTTTTTAATTATATTAAAAATTAGCGTTTTAAAATTCATTTTTTTAAAAGCATAAAAAAAGTTTGCAAATATTATTGCAAACTTCTTACTTATGAATTAATTATTTGTTTGTAATTCAATAACATTATTATTTGAAACTGTTAATAAGGCAACATCACCTTCAACTAAATTTCCTTTTATGATTTCCATTGCTAATTTGTTTTCAATGTTTTTTTGAATATATCTTTTAATTGGACGAGCGCCAAAGGCTGAATCGTAGGCGTTATCAGCAACGAATTTAACTATTTTGTCATCAAAATTAATTACTAAATTATGGTTATTAATAACTCTTTTCTTTAATTTTGATAATTCTAGTTTTACAATGTCACAAACAACAGCAGGACTTAATGGGTTGAATGGCACTATTTCATCGATTCTATTAATAAATTCTGGTCTAAAACTTCTTAATAATAATGCCCTTACCCCATCAATGGTTAAACTCTTATTTATAATTTCACTACTTCCAATGTTTGAAGTCATGATAATTATTGTGTTTTTAAAGTTTACTACCCTTCCTGTACTATCTGTTAAGGTACCATTGTCCAATATTTGTAACAATATATTTAAAACATCTGAATTTGCTTTTTCAATTTCATCAAATAAAACTATTGAGTAAGGATTTTGTCTAATTTTTTCAGTTAGTTGTCCCCCGTTATCATAACCAATATAACCTGGAGGCGATCCAATTAATTTTGACACGCTGTGTTTTTCCATATATTCAGACATGTCTAATCTTACAATGTGATTTTCATCATCAAATAAAGCTTCTGCTAAACTTTTTGCAAGTTCAGTTTTACCAACACCAGTTGGCCCTAGAAATAGGAACGAACCAATTGGTCTATTTGGGTCATTGATGTTTGCTTTAGAACGTTTGATTGCATCAGAGATTAGTTCAATTGCTTGATCTTGACCTTTCACTCTCTTTTTTAATGTTTCAGACAAATTCAAAATTTTTTGTTTTTGGTTTTCTAGTAATCTATTAACTGGAATATTTGTTCATTTTGAAACAATGTCAGCAACTTCTTCAGCATCAACAACTTCTTTGATTAAACGATTTTTCCTTGATTGTATTTTTTGTTCCAATTCATCTCTAGTTTTAATTAATTCAGGAACTAAAACATACATAATTTTAGAAGCTTCGACATATTTTCCTTCACTTTGTAGTAAAGGCAATTTTCTATTCAATTCTTCAATGTTTTCTTTAATTGAAGAAAATTTTTGAATATCTTCTTTTTCTTGCTTCCAACGTTTTTCAAGGTCAACTTTTTGCAATTTTAAAGTATTTAGTTCTTTATCTATTTCTAAAATTCTTTCTTTATTTTTAACGGCTGTTTGCTTTGTTTCACTATCATTTGTTAGAGCAGCTTTTTCAATTTCTAATCTAGCAATTTGATAATTAATTTTTTCTAGAGGTTCAGGCAAATAGTTCATTTCAGTTTTTATATTTGATGCTGCTTCATCAATTAAATCTATTGCTTTATCAGGCAAAAATCTATCTGCAATATATCTTGCTGATAAATTTGCTGCCGAAACTATAGCAGAGTCATCAATTTTAACTTCATGATATGCTTCAAATCTTTCTTTTATACCACGCAATATAGTTATAGTATCTTCAATTGAAGGTTCTGAAATTAAAATTTTTTGCATTCTTCTTTCAAGGGCAGGATCTTTTTCAATATATTTTCTATATTCATCCAATGTTGTTGCCCCAATTAGATGCAATTGACCTCTTGCCATCATAGGTTTCAATATATTCGCCGCATCCATATTGCTATCGCCAGTTCCTCCGGCGCCAATTATCATATGAATTTCATCAATGAAAAGAATGATTTCACCATTTGCTTCGTCAATCTTTTTCAATACTTTTTTTAGTCTTTCTTCAAATTGACCACGATATTGTGTTCCTGCAACCAATGCTGGTAAATCAATTTCAATTAATTGTTTACCTTTTAGGTTTTCTGGAACTTGATTTTCAACAATTTTCCTTGCTAGGCCTTCAACAATTGCTGTTTTACCTACGCCGGGTTCGCCAACTAAAACGGGATTATTTTTAGTTTTTCTTGACAAAATTCTAACTAGTGATCTTATTTCTTCATCACGGTTTATAACAGGTTCTAGTTTGTTCTGCGTAGCTAACTCAGTTAAGTTTCGACCAAACTCCTTCAAGGGATCTTTTTCTTGTTCATAAGGTGTATAACTTGCTTCCATATATTGTCCTCCTTTTACAATAAAAAATTATACTACAAAAATTAGCACTGTGTTAAAAAGAGTGCTAATTTTTTAATTGATATGTTGCCCATAAAAAAATTTGCTATTAAAATAATTTTATTTTTTTATAAAAAAATATTTTAGTGAAACATTATGTTTTTTTATTCTATAATTAATCCGGTTATTTCGTAACCGATTGAGTTAAGATGAAAGACATTATAAATAAACGATTTTGATTTCTATTTTTATTTATAAATGTTGCTACGCTTTGCATTTTTTTAGGAATTGCTTATCTAAACTGAGCTCTACTAACAGGATATTTGGTAGGAGTAATCAGTTTTCTTATTTTTTTAAGTGGCTTACATTTAGTATTTAAAAAAATGAATGATTGAAAAGAAAACGCTAGTATTAAAAAAAACAAAAACCTCGCAGTTATTATTTTTTTAATTTTAAATTTTCTTGCTTTATTGATTATTGCCTTATTTGTAATCTTTAACTTACTTTATAAAAACAAACATTCAAATGCAAATGTTGCATTTGTACCTTTTAACGTAATCACAATGGCTATTCCATATACATTATTTAGTTTACAAATAATAGTTATGGAATTAATTAAAAAAATTACCACTAAAAGGAATTTAAAGAGAAGGGAGGAAAATGGATAGAATTTTACGTCTTCATTGATTTGGTGGTCCAGTTAATCCTGGCGAAGTAAGCAGTTTCAATGACAACCAATTATTTACTTTAATTATTTTAGTTGCTTTAATTTTTATTATTTCAATACTAATTTTTGTAGCAATAAAAAGACAAAAAGTTGATAAAGCACCTAATACAGTTGTATTAATGGTTGAAAGTATAATAAAAAACAGTGATGAATTTATTTCTGAAACCCATGGCAATAAATTTGACAAAATGAATCCTTATTTTATTTCATTATTTGCCTTCTTGATTTTAGGAAATATGCTTTCATTAGTTGGTTTAGCCCCAATTGGAACTAGTTTGTCAGCAATTTTTGCTGCCACTGTTATAAGTTGACTTGGAACACACATCATTGGATTCATATATAAAAAATTTAAGTATTTATTTAAAATTCTTAATCCCTTAGAAGCATCATCAAACTTTTCACCATTAATAAGTTTAACTTTCCGTATGTATGGAAACATACTTGGAGGAGTTGTATTGGTTGCACTAGTAAGTTTATTCTTAAATAATATTTGAACCAAAATTATTGGCGCTAACTTAGAATCACCTTCAGCTGAGTTAAACCCTATTGGGTTATTGATCTTGCCAGCATTTAATTTATATTTTGATATTTTTGATAGTTTCATTCAAGCATTTGTATTTATGATTTTAACAATTTCATATTGAACGCAATCTGCTGAAGAAGATATAAAAGAAAAACATAAAGACAAAATAAAAGAATGAAAACAAGAAATCTTAAAAAATAAACAAAATAAAAATAATATAGATAATAAAAATAATGAAACCTCAGTAGTAGAGGCAACTATTTAGGAAAGGAAAAAAAATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTAAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTTTAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAAAAATATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTTAATTGACCATTCTTTGTTTTTTCATTAATAAGCTTAGGCCTAGTTGTTTTGATTATTACTTTATTGGTTTATAAACCCATAAAGAAATTAATTAAAAACCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAGGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCAGAAAGTGAAAGAATTGTCAATGCAAGCACGATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCGCAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAATATGCAAGATTTTAATGAAATCATTTATAACTGAGCATATGCAATATATGATCTTACAAATGAAAACAAAAATTTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTATTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCATTTTCAAAATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAGGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAAGGAGGGAGGAATATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATAGGCCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCCGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGTAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTAACCATTTATCTGGCCGTGTGTTAGATGCGCTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAGATTGCACCTGGTGTTATGACTAGAAAAGAAGTTAATCAACCACTTGAAACCGGGATTTTAGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGTAAGACCGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAACGTTTATTGTATTTATGTTGCTGTAGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGAATGGCAAGAGGTAAGGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCTGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCAATAACTGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGAACTAACGAATTAGTTGAAAAACTATATAATGAAATAATTAGCTTTAATGAATTATTTGTTAAAACTATTCCATTATATAATAAAGATTTATATCAACCAGTACCTGAATTACCATGGAAAGTTTGCAAAAAATAAAACATAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTAGCAACTGCTAAGTTTGCTAAGATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACTTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGCGCTTCTTGAAAATTCAAAAGCAAAAGAAATTTATATATTGTATTTGGCAGTGATTTAGGACTATGTGGTCCATACAATTCAGAAATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGCAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCTGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGCCAAAGAAGAACGGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATACAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAAAAAGAAAGGAGTAAAAATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGTGTTATCAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCATTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGTGTATTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATCGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTCGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGGGAAGGTAATGACCTTTATCACGAAATGCAAGCAAGTGGCGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTCAGATTTACACAAGCAGGTTCTGAAGTTTCTGCATTGTTAGGTCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACCTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTAGATCCAAATATAATTGGCCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGATGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGACGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGGCAGGCGCAACTGGGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCACCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCTGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAATTAATTTTAAAAAAGTAGGAATTTAAAATCCTGCTTTTTTTATGCTAATTTTTAAACTAAATTTTTATAATGAAAAATTTATCAAATTAATAGTTTGATTAAAAAGAAAAATTTCTGATTTCAATTTTTTAATTTTAAAATATTTGGGCCATTTTTTATATGTTTAAAATTTTTAAAATTTGTAATTCTTTTTATAAATTTTAGAATTATAATTCCACAGTACAACAAAAAACAAATAAGAAGGAAGATAATAAAATGAAAAAAATAACAAAAGCTTTATTAATAATATGCACAACTATAACACCTAGTTTTGCTATACCGTTAATATCGGCAGGATGTAATAAAACAACAAATCATGAAACAAACAAAGAAAACACAATTATAAATAGACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAACCCCAATAAGTTAGAATCAAATAGTGAATTAGCAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGATTTCAATGTTTGACAAATTAAACAAAGAAAACTTGTCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTCAATGATGGAGATAAAATCGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAATAACGAAAGCTATTTAAATGGATTATTGAATGATAATGACACAATGTTTAATTTAACAACTAATCTCAAAAAAGGTAATGAAATAGATGCATATTATTTAGTTGATGGTCAAGGTTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGAAATGAACAAAATGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACTAAAGACAATAAAACTATTACAAGAAAAAATGTAAAAATTGTTAATAAAATTGCTGAAGGCGTTTCAGATGATTATTTAATAAAATATAAAAAGTTAACTTTAAATTATAAAGACGCTGACAAGACTTCAATAGATTCTTTTAGCAAAGGAAATATTGAACAAACATTAAAAAATAATTTTATTGTTAAATTTGAAAAGAGCAGTGAAGAAGAAACTAAAAAATTCTTAGAAGACAATGGAATTAAATATGAAGTTAATAGTATAACTGAAATTTCTCTTGAAAAGAAAACAATCAATGTTAATCTTCAAACAACATTAAAAAATAAGAAACTTGTCAATGCAAATATTGAAGTTGCAGGATTTAAAGGCATTGAAAAAGTAAGCGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAGTCAAAGTCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAATTAGAAAATCCTTTAGATTTTGCAGAATATTTTGCCAAAAATGGAATTAACAATTTTGATAAAAATTATATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAATGAAGTAGGTAAGGAATTTTTAGGAAATCCAAATGAAATTTTGGCAAGTGATTTCTCAGCTACAAATTTAAAATTGAATAAAAATGTTGGCGATAGTCTTGATAGTTTAAATTTTATTAAATTATCTTCAATAAAAAGACCTTCAAAAAATTCAGAGGAATTAAAGCTCAACGAAGAAGGTAAGGCAACGTTAATTTATGAATTTAGAAATATATATGATAATTCAATATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAAGTAGAAAAAGCTGATTATAAAAAACAACACACCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGTGGTTGGGCAACTGCCGAAGAAATAAACGAATTGGAAACTTTTTTGCCAAGCCAAAAAAAATCTAAAAAAAATAAGAGAATAAACATTGAACATTATATAAACAATGGCAATATAGAAATACTTGTTGGAAGAGTAATTACTAAAAAAAATAAAATTACTTTCGGCGATGCATTTAAAAGACAAAAAGAATTTAAAGAAATAGTATTTGATGAAGAATTTGTTGAAGCAGTTAAGAAATCATCTAATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGATGAAAATTCTAAACATAAAAATCAAAAGAATTGAATAAGGCTTACAAAACATGAAGGAAAATGATGTATACTATTTGCATTCATTGAAGATACAAAGTCAACACCATATTATGTTCAAATAGAAAAATAGTCTTATAAAATTTTATTACAATAATAAATGCAACAAAAGTATTTAAAAAGTAGGGGATTGGCCCTACTTTTTTATATTAAAATAAAATAGTAAAACATAAAAAAATAAGGTCAAAAGTTTTTATTTTTAACCAAGGAGTTTAAAATATTTGTTTTTTGTTAATTTTTAAGAAAAATGCTCATTTTTAATCACCTTAGCAAATAAAATTATTTTAAAATCAAAAAAAGGAAATACAAAATATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGGACAATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCTAAATAAACCAGAAGGTGAAAAACCTGGAACAACTCCTGAAAAGAAGCCTGAAGTTAAGCCGGCGGGTGAAAACCCCGGAACAACTCCCGAAGTTAAGCCAGGATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGGTTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAACAACTTCGATAAGAAATTCAAAGTAGAAGTTTTAAAATTAGAAGTAGATAAGAAAATTAATTCAATATTAATTACATACAGCATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAACAATGAAAGCTATTTAAGTGGATTATTGAACGATAAGGATACAGTATTCAATTTAGGTGATGCTTCTAATGAAGATAGTTTAACAACAGTTGAACAGTTAGATGCATATCATTTAGCTAATGGCCAAGGTTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCTAAAGATCTAATCTTAGAATATACTTGAAATGAACAAAATGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAATACCAAAGACAATAAAACTATTACAAGAAAAAATGTAAAAATTGCTAATAAAATTGCCGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAGTTAACTTTAAATTATAAAGACGCTGACAAGACTTCAATAGATTCTTTTAATGAAGGCGATATTGAACAAACATTAAGAAATAATTTTATTCCCAAATTTGAAAATAGCAGTGAAGAAGAAACTAAAAAATTCTTAGAAGACAATGGAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAACGTTAGTCTTCAAATAACATTAAAAAATAAGAAACTTGCCAATGCAAATATTGAAGTTTCAGGATTTAAAGGCGTTGAAAAAGTAAGTGATGAACAAGTTAAAGAAATAGTAGATAAATTAACTTTTGGCGATGTTTATTTCCCTGAAAAAATAAAAGATTTTAATCAAGAATTTTTAAATGAACTACCTTTATATATCAAAGACAATGATGGAAAAATTAAACCCGAAAATACACTTGATTCTGAATATTTAATTAAAAACGGAATCACAGAATTTGATAAAAATGAATTTGGACAACCTAAGCATGAAGTCAAATATGATGAAAATACAAAGAAATTGTCCGCAACATTAAAATTAAACGGCAAGGAATATAATGTTGAAACAACATTAACTCATGAATTAAAATTAGATGAATGGGTAGATGAAGTCGGAAAAGAAATTGATGGGGATTCAAATACCATTCCCGTAAATGAATTTTCTAATAAAAATTTAAAATTGAATAAAAAAATTAATGACTTCGTCAATGGTTTAGACTTTGTTCAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAGGCAGAAAAAAAATTGAATTTAGAAGGTAAGGCAACCTTAATTTATGAATTTAAAAATATATATGATAATTCAACATATAGAAAAGAAGTCGTATTCGAAAATCTAAAAAAAGCAGAAAAAGTTGATTATAAAAAACAATATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGAGCTTCGCCAGAAGAAATAAAAAAAATAAGTGCCTTTAAAAGCAATAAAGAAACCTATTCTTATAAAGATAATGAAGGTAAAGAACAAAGTGCAGCATTAACTGGTAGATTTAAAATTAAAGGAAAATCAGGTGATTTAAAATTGCATTTTGGAGATAATAGCAAAACTAAAGAATCATTCAAAGATTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAGGATTCCGGACATGGGGAATGAGTTAGATTTACACAACACAACGGCAAATGATGTATAGTATTTGCTTTTAAAGATGATCCAGAACTTACACCTTACTATGTTCAAATAGAACAATAATAAGTTCAAAAATAAAGTTCATTCTATTAAAAGAAAGGACTTTATTTTTTATTTATAAAATAAAATTTATATATTATAATAAATTATTACAAAAAGCAAAAGGTGGGTAAAATGGTTAAGAAAAAAGTATTATTTAAAATATTTGCTATGGGTACTATTCTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAATGATACAGAAAATAAAGATAATAGTTTTGAAAATAACACTAATAACGGTCAAAGCAACCAACATGATTTAGGCACCGAAGATAATAATTCAAGAAATTTGATAGTTCCTGAATCAAATAATAAGCCCAAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTACCCCAACGCCCGAACCAGCGCCTGAGTCAACACCTGAAATAAAGCCTGAGCCAACTCCCAAACCAGCACCCGAACCAAAAGAAAATGATCATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTACTAGAAAAAAAGGAGAATACATTGAACCAAAGCCGTCGATTTTATTGGCTGATAGGTCAAATAAACAGTCGGATTTGAATAAACAAGGAGCCCAAGAAGTAAGCGAATTCCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCTAATTTTGTTATTTACGGTGGCGATACTAATATAAAAAATGAAAATTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAAGCTTAAAATTAAAAAAATATGATCAAAAATTTCTAACATCATTAGGAACAAGAGGAAAATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTCAAACCAAAAATAATTACCAATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAGCTTGAAAAATTAAAAGGATGAAAATCAAAAGACAGTGATAAATCTACAATTAGAAGTCAAATTTCAGACCATGCTCCAGTATTTACAGATGTCGTATTTTCAAATCCAATTGAAACGCTAAAAGTAAATGAAACATTAAGAAAAAACCAAACTTTATCTAAAGAACAAAACACTATAAGAATAGCTCATTGAAATATACTGAATTTTGGCGGTCCTAATCAGGATGCTAAAACAATGGCTATTGCTACTACAATTTATAAGTCTGGTTTTGATATAATAGGCTTAACTGAAATTAATGATGGAAGAGGAAACTATGTGCCAAATATAGTTAACCTTTTAAATAAATATGCAGGAAAAGAAAGATATAAATTTGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCGCAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCGAAGCTATTTAAAACCAATTAAATACTACAAAGATAACTAAAAAAACTTGGATGCTTCCAAGTTTTTTTAGGCTAATATTCACCTTGTTTAATTTTATTTAATCCACTTCATTTGTTAATTGAAGTTTTGTTATTCATATTCATGAATTTTCTATAACCACCTTCTTTGATAGTCATTAGAATATATTCTTTTTTAGAAACTTCATTAGGTTTTCCGTTTTCTTTAACTTTGAAGTTTACGTTTTTTAATTTACTACTTTCAGATTTGTTATATCCACTTGTTACAATTAAATAAGTAATTGATTCAGGACCATAATTTTCTTCTTTATAGTATTCTCAGGAATGAATAAATCATCCTCCAGTTTCATTATAAAGATAGTTAGTAAATTCTCTTCTTCTTTCAGAGTCTTTAAAATAAGCCTGCATTCCTGTAAATTTACTTGATACTCCAGGAATTGCTTTAAGATTAGTTCCTTCTTGAAATTCTCAAAGTATTGCCCGTGTTCCTTGATATAGGCTCAATTTTGGCGCATTTCATTTTGTACCAGGTTCTCTATCTTTTTCCAATGTTTCTCTACCAAACATTTCAAATATAGCAAAATCTACTGCCACATTAAAACGATATACTGCTTTTCATGCTTCAGATTGTGCCCTAACTTTATATGTTGGTGAAGTTGCATCCTTATCATTATTGTATGCCTTTGCAAGCAATTTATAAATTGTATCAAGCAATTTTAAAACATCTGCGGAATATTCAATATTATCAGGGTCATCTTTATCAGTGGCTTTTGCAGATTTGAAAATGCTTCCTAACGCATTTGAAACAGAACTATTTAAGGTATTCATGTTTGAACGTTTTGATTCATCTGAATTGTTGTTTAAATTTCTTAATGCATTATAAATTACAGAGTCATTAAAATTACTTTTTCCAAATACTAAATTGATTAATTTAACCAAAGCAGTTTCATTGATTGGTTTATCTTCGCCTACTAATTCAGATAATACTAAAACATCATTGAAGGTAAATGCCGATTTAATTTTTTCAATTGCATCATTCAATCATTTAACAGGGTTTAGAATATCAAATTGTTTAGCGTAATTGCTTATCAATCCTATTGAACGCTTAATGAATTTCCTTTTAATAAAATCATTATTTAATAATGCCGGCACGATTTTTCCAATTAAGTCTTTTAGGAATTTTTTATCTTCATCATTTCCTTCTATTTTTAGATAATTTAAAATACTTTCAACAATGTGATTTGTAATTTCATTATCAGTTGCAAGATGCTTAATAAGTTCAGAAGAGTTCTTAATTAGATTATCTTGAATTCTAGTAATTGTTTTAGATATTAAGTCCCCAAGGTTGGTCGATTCAATTTTTGATCCCGCTAGAAAATCATTAACTACGGCTTCAATTAAGCTTTTAATTTCTGGAAAATCAATGAATTTTTTACTTATTAATTCTAACTTATCTTTTGGAATAATATTTTGTTTAAGGCCATCTCAATTAAATTTTTTAATTTGATCCTTCAAAACATTATATACAAAATCAACTAATGCTTCTTTTCCATTTTTATTATCTGCTTTTATTGATCCATTTAGAGCTTTTAAGGCATTGTAAATTAATTTTTCTAGATTCTTTAGGAATTGTTCGTGTTCCTTATCTTGTTTAGTTTTATCTACTTCGTTGAATGTTGTAGAATTGCTTTTAGCAGAAAATGCTTGTTTAAATTTATCAAGATTGTCGACTATATATGAATAAGCGAAATTCAATTCATTATACAAGACATTTTTATCAATATTTTTGTCTAATATTGTTTCAAATAATTCACTTAAATTTTCTGTTGTTAAATACTTATGGTATTCAATCTTTTTAATAGATTCAAGAAGTTTGGATGCCAAAGCATTTCCATTGAAATGACCATTATTGTCAAGGAAATCAACATCAACAAATAAATCAATGAAATCTCCCAATATATTGCTAAATACATTCATTGAACCTAGTTTGTTAATTAATCTAGTCAAAAGATTTGCAATTCCTTGAATTTGAGCGCTACTTGAACTAATTGAATATTTCTTGTTGAATAAATTCAATGCATATTCAATAGATTTGTTAATTAAGTTTTTGTTAGAAGTTATTTCATTTACTGCATTTTTAATAAATGCTTTAAATTTATCTTTATTTGCTGACAATAAATGTTTAAATAAACCATTGAAATCACTAATAGAAGCTCATTGATTGTTATCGCCTAAATCTATCAATGAATTTAATAATGTTAGTATAAATTCTTTTGTATTATTTGAATCAAAAATTACCTTCATTAATGTTTCAACTATTTCATTGCCTAATTCAGAAGCTTTTGAATCAATTTTTAGAATTTTTTCTTTTATTTTTAATAAATTGTGTTTGATATATTGTTTAAATTTATTACTTTGTAAGAAAATATCAACTGAATTTTTTAATTCATCTTTAATTTCTTTCTTTGCTTTATTATCTAATATCGAAAATACGCATTTAAACAAATTAAAATAAACATTGATATTTACCTTGATTTGTCCATCAAATCCAACTATATTTGTGCTTGAAGAATCTAAATTTTTATGTTCAGGAAGTACTGGAGATTCATTGCTATAATCTGGAATAATATTTTCAAAGTTTTTGATATTTTTTAATAATAATAATGCAATATTTTTTATATCTTCAGATTTTAAAGATTTAACAAATTCTATAATTTTGTTGTAGTTTAAATTATTAATAATGCTTACAATATTAATTAAATTATTTCAATCGTACTTTTTAAATTCAAATTGATTCTTGTTTAGATTATTGACTGAGTAGTCTAAAACATTTTCAATTAAGCCATCTAGAATATTGAATTCTGGCAATTTTTCAATACCTAGCGAAATTAATTTTAGTAATGTTTCTTTTGCATTAACAGGCCATGTAATATTCTTTAGATTTTGTTCCATCAATGAAATTAATAATTTTGCTATTGAATTTTTAATATCAATACCTAAGGTTTTGTTATTGATAAATTTATTGAATATTTCTTTTATTTTTTTAATAACATATTCTTTATTATTTGAAATAAATTTGCTTATTCATTGAGAAATATTATTTAAATCTTTTAAATTATTATCATCAAATAACTTTTTAATTAGCTCTTTTATTAAAGGTAAAAGATCTTCATTGTAACTTGTTTCAACAAATGATTTTATATATGAAATTATTTGTTGAAGGTTTTCTTGTACATCACCCTTAAAGCTTGAAATATTTATTTCATCAATGTATTTCAAAATGATTTCTTTAATATTTATTTTGCTTGCTATGTAATTTATAAATGTTTCTTTTTCTAAAACAATTTTTAATATTTTAATTAGTTCGACATCTTTTATTTGGTTAATTTCTTGTTCTTCATATTTCTTTGTTAAGAATTGTTGAACATCCTTAATTAATTCACCATTATTAAAACTAAAATAATTCTTAATCGTTTTTGTAACGGATTCAAGAATAGAAGCTCAAGAATATTTATTTATTGACTTCAATTCATTTGTTAATTTAATTTTTAAGTTTTTATATAAATTAGATTTCAATGAAGTAGGTAAAATCTTGTTGAAATAATTAGTTAATTGGTTAATTTCGTCAGAATTCAATTTTGTTTTTGCTTCAATTTCTAAAAACGCAACTAAAAATTCAACTAATTTTTGGGTTATATCTTTATTTGATAAAACATTTTCTAGCAATTGTTCTAACGCATCAAAGCCCTTGTTGTCTGCAAGATTTTTCAATATTTTATTGATAAATTCGGGCAATGTTTGAGCATCATATTCACTGCCGTTTTCAATTAAATTTTCAATAAATTCGAAAACTTTGTCAATTAATTTATTATCAATAATCTTATCGAATTTATCGACAATAAAATTGGCAAGATTATTATCATTTGTATTGACTTTATTTAGAACTTCATTATGAACCTTTTTTAACTCATTATTAATTATTTTTGTTAAATATTGTTTTAAATTTTCATTTTTTAATAACTTTTGAACTTCTGGTTTTAGCTCATTGATAATTGTAGATTTAGATGAAGCTAGTTTATTTCATAATTTTTTAATTAGTAAGAAAATTTGCTCAAAATTTAAATCATTGTCTAATTTAATTGGTTTTGAACCTTCATTTTGTTTTTGTGTTGTTGTGTCGTTGTTTGTTAAAAAAGCATTTGCAAATTCATTTAAATTCTTGATTAATATCAAAAATAGAGTTTTTACATTTTCTTCATTTAATGTATCTATCAATGAATTTAATTGTTCGTAGTTAAATAAATGCTTAATTAGATTTTTTAGATCTAAAGCATTGCTTCAACTAATTTTGTCTAAATTAAAACCATTATTTTCAATGTATTTAGAAATATACGAAGCGAATGTTGATACTAGCAAGTCAACATTTTGTTTTTGCAAGGCAAAATCAATCAATTTTGATACTAATTTTTCAATATTGCTTTCACTATCTATTCCAGCAAGAAATTGAGGATATTGTTTCTTTAATAATTTAAATAAACCACCTTTAATTTCTTCGTTGCTTTTTATTTCTTTATCTAAGAAATTATTTAAAATTTCTTTTACTATTCCTACATTTTTTGAAATTACATTTGCAACAAATTCATTAATGTTTTTAGATTCTATAACCTTATTATCTATTGCAGAAAGTTCAATAATTGATTTAATTTTTGGAAGTAGTTCGTTTTCTCATTGACTATCTATGTAATTTCTAGTTTTGGTGATTAAGGTTTGTAATAGTGAATTTAATTCAGTGTCGTTAGATAGTTTGTTGTCTTTAAATGAATCCAAGTAATTTAATATAATAGATTTAATGTCAAATTTTTTAAAGACGTAGTTAACAAGTTCAGATTTAGAAGCAAGAACAGAAAGTGTTTTTATTAATTCAGAAAATTTTATTCCTTCTGATTTATTATCTTTTTCGAGTAGTAAATCAACAAAATTATTAATTAAATCCTTATTTTTTTCAATGTTGAAGTAGTCACTAAATATTGATTTTAAATAGTTTTTCAATTTATCAAACGTGTCAATTTTTTTATTATCTAAAATTTGAATTTTTTCATCAAAATTTTCAATAAGTTTAGTTACTAAATTGCTTTTAGTAATTGAATTGATTAATTTAATTAAATAATTGCTTATTTCTTTAATTTCTTTATCATTTAGTTCAGTATTTGCTTCTTTTTTTAATCAAATTGCGCCCAATTCAACTGCTTTATCAATAAATTTGGTGTTAGTTGCAATTTCTGATATCACCGTTTTTAATAAATCAATTGAACCATTCTTTTTAGAATTTTTTAAGAATGAAACCAAAAAATTATAGCTAGACGAGAAATCGTATTCATTATTTCCACTAATAAAATCATTTAAAATATTTTTTAAACAACCTAAAACTTTCTCACTTCTTATTGTTGAAATTAGTTGATCTCAAACATTTTCAACAAATTTTTGAATTTCTTCTTCATTGTTGAATATCTTTTTGTAATTAATTGATTTAATGAAAGCAACGATAGGCTGAATAACAACATAATTTCCAATTTGATCAATCAATTGACTGAACAAACTATTATTCTTAAATAATTCATTTACTAGAAGTGAGATAATATCTTTAAATATTTCACTACCAAATTCGTTTAAATTCTTCTTTGCATTTAATGCTATATTTTTATAAAATCTTAATATGTTTTCTCTTGAATCTTTATCAATTAATTTGCCTATGTTTTTTGGCATTAATGCATTATTTTTAATTGCATATTTTAGGGCTAAGTGTTTTAATCCTTGTTTGTAGTCGTTAATATTTTGTATTTTTGGTTCAAACGATAGGATTTTAAATAAAAGCGAGTTATCCCTATTTAGGTCGTATTTATCAAAAAGAATTCCATTTAGAATTTCTGAAATTAAATCCCTATTTTGGTGGTCATCATCCAGTTTAGAATTCATTAATATTTGAATAATAATGTTTGATATTTGAGATAAACCATCAAAGTTTGAAATGTTGTCAAAAAATGTTGAAACAAACAAAGAAATATTTGGTTTTTCAAGTAAATCTAAATCATATGATTTGTATAAAGAATCAACAATTTTCCCGGTCAAGTAGTTTGCAAGCTTTCTATCTTTTGAAACTTCTTTAATTATTAGGCTAACTAAATCATTTACATCTTTGCTTGAATCATGAACCAATGTAGTAATAAAGTCATCAATTTTTTCAAATTTAAAATATTTATCTGAATTATTAATTAACAAATTCAACACTTTTTGGAAAATGGTGTCTAAACCTTTTTCAATATCTTTGTTATTGAAGAGATTTTTGAATAAATCAATAGTAGTTTGAGGCAATAATCTCTTTAATATTTTTTTAATGAATACATCTGAAAATATGGCCTTAAATAATTTTGCATATTCGCTTCTTTTAAGATCTTGACTTAAAGATTTGTTATAAAAAATTTCTTTTAAAATTTCATAAATATTTTGTTTAGAAGTAGTTGTTGAATAAACAATTTTTTTAATTTCTTCAAAGTTTAAACCTGCATATGATTTTTTATTAATAGCATTATTTAATGCGTTATTAATATCTAAAAACGATTTGCTTAAATTTTTTGAATCAATAAAAAGATTCAAAAAATTAATAATAGATTTTTTACTTTCTTTATTCTTTAAAGATTCTAATAAACACTTATTAAAGCTGTTTAAATCTTTTGGGTCTAAGCCAATTAATCCAAATAGTATTGAAAATGTATTCAAAATTTCTTTATCTTCAATGTTTTTGTTTGAATTTAACATTGATTTTAATTGAAGGATTATATGCTTTGAATTGTATGAAGAATTAATATTTTTGATATCTGCATTTTCTTCGAATGTATATTCTTTTTCAAAAGAATCTACATTGTTGCCATTTATTCCTAAAATCATTGAAAAAATTTCGGAAGGAGTTTTGCTAAATTCAAGTGATTGGCTAAATCCATTTAGATTAGAATTATTATCAACTAATTCTTTATATTTATTATTAGGCAATGACATTTTCATAAAAATATCTTGCGCTAATTTTTTGTATAAATTATTAGATGGGAAGCAGTCAAAGAAATTGTTGTTTAGTTTATCGGCATTATCTTTGAGATTTTTAATGTCATTATTAATGTTTAAATAGTTTAGCTTGTTTGTTTTGGCAATGTTTGCTAATGATTTGTTTAATTCTTCTATGCAATCATTAGTCAATGAAATATTGTATTGTTTGTCAATTAATTTTGTTAATTTCAAATAAGGTGATATATAACCCACAAGATTAATATTTGCCTCAGGATTATATTGTTTAATGATTGAAATTAATTTTGAATATGAATCTTCAAAATTTTTACAATTTCTTTTAAATTCAAGAAGAATTTTTGAAAAATATTTCAAAATTTCTTCTTTATTTTTGTTTTCTCTTAAAAGATCAAACGCTTCTTTAAAAAATGATGATTGTTCAAAAAGGTCGTTGAATCCCAAAGAAATTGTAATCAGGTTTGCTTGTTTTATTTTTTTAATTAGCTCTTGTAATTTTTTATTAGAAAAATCACCAAATAGATTATTCAATTTGGTACTATTAAATTTTGAAAGTAAATTTTGATTATAGGAAATAACTTGATTTAAATGAGCTAATTCTTTGCTGTTATCTAGTGAAAGCAATGAAATTCAATCATTAACATTTGAAAGTGGCAAACCGTAATTATAATAAGTTGATAATGTTGTTTGTTTATCAGATAATAGTTTTATAAAATTAGCAATGTATGAAGGATACGATGTTCCTAAAACTAAATCGTTTTTGCTATCATATTCATTGTTGAAATAGTAATTTGAGCTGCTATTATAACCTGCAGCAAAGCCGTCGCCTAATGCAACATAATTAATTTTTTTAGAAACTTTATTTTCACTTTGAGTTATTTTTGTATCCGGCTTCTTAGTTTTGTCTTTGCATGATATAGCAACTAATGAACTAGAAGCAATTGAAGATGTAATTCCTAAAGCAACAATCGCCTTGAGGATTTTTGTACTTTTTTTCATAAATACCTCCTAACCAATAGAAATATCTTCATAAATATATTGATATCTTTGTGGTTTTGATTTTGAAAAAGTAAAAATTTTTAGAAATTGTGATATTCCTAATTGTCCTACTATCATTGTTATAATTAAAAGTATTTTTCCAGTAATACCCAAATTTGGTGTTAATCCAATATTAAGTCCTGAGTTTCCGAATGCTGAGAAAACTTCGAACATTACATATACCATTGGATATGACTCATTATAAACATTTTTTAAATCGCTTGGAAGTGTATGATATATTACTATTGTCATTATTAGAGTTATAAAAATTGCAATTGCAAAAATGTTAACTGCTTTAATTAAAGATTCTTTTCCTATTTGTCTATGAAAGGCATTTATGTTTTTGCGATTTTTAATTAAAGTAATTATACTCATGAAAATTATAGCCACTGTAATATTACGAATTCCTCCAGCAGTTGAAACAGGAGAAAATCCAATAAACATTAGGATACCGTGAACCATAATTGTAGGTTGAGTAAAATTATAATAATTTATAGTTGAAAAACCTGCTGATCTTGTTGACATAGTTTGAAAATATATTGCAAATACTTTGTCGCCTATCGAACCATATTCGCTTGAGTTTCAAAAATAATTTGCACCCTTAGCAGTTGTTTCAAAGATAAATGTTAATAATAAACCAATTAGTGACGTAAAAATGTATGTAATAATTGTAAATTTTGTAAATAATGTAAAGCAATGACGAGGATTGTCTTTCTTGATGCTTTTAATTTTTTGTCAAACATCATAGAATACAGGGAATCCCATTCCACCAATTAGAAATATAATTGATGTTAAAAATAAAAAGTAATAATTATGATAATAAGGTTGCAAACTTTTTGTGCCAATTATATCTAATCCAGCATTATTTATTGCACTTAGTGTATGAAATATTCCAAATCTTAGTGACATACCAAAGTTATGATAAGGATTTCTTATTGATTCTTCATAAGGATTAAATGTTCATGGATTATTTTTACTTAATGCATTTAATTGAGACATCTTTTCATCACTAAAAAGTGCTGTATTATCATAATAAAATATTAAAGAAAATATTAAAGAAAATAATATAGAAATAATAATTAAAAAACTTATTGAAACTTTTATCATTTTTTTTGTTTCATAAATATTAGAACCGCCTCTTTCAGTTTGGTGAATTTGGCCACTAAATAAATTCGTTCTTAATCCAAGTAATGATTGAAAAATATAAAGTTTAAATGTAAAAATTCCAATTCCGCCAAGAATAAAACAGACAGCTATTAATATCTGTCCAAACATGTTAAATACTTCACTAACGGCAACTCCACTTAAACCGGTTGTTGTAAATGATGAGGCTGCTAATAGCAAAGCGTCAAGATAACTAATATGCTTTGAGGGATTATGACTAACTTCTCAATATAACATCAAAGACATTAGAATCGTAAAAAGTATATAAATCAAAAAGATAAATCTTATTGTTCCAAGTTTCTTTAGAAACACAAATAAGACATTTGATTTTTTTGGATTTCCCTTTTTAATCATAGAAAGAATTTTACCACAAAGTAAAAAAATGTTATTTTATAATACTTTACCATACCGCTTCTGTGTAAAAAAACTATAATATTTAAGCTAATATATTTAAAATTCAAAATTATTTTTTAAAATATTAAAATTTTGAAATGAAAAGAGGTTATTATGAGTTTATTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGTCGGTAGCAAGAACATTATTGGCTGATAGAAATAATAATATAAGGATAGTTTTAGTTGATGAAGATGAAAAACATTTGTTGCCTTTTAAAGATGAAGTAGAAATGATCTATGTTGCCGATTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCCGTTATTGTTGGCGCTTCAAATAATATTGAAATAGTCGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGAGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGTCCTGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGTGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGCCAAATTGAAGATATTATTCAAGTTATTGAAATTTGTGCAATGAATGAAAAATAACTTAAAACAAGAACAAAAAAATAGCAAATTTTTTCTAGTTTGCTATTTTTAATTTTAATTTGTAAATATAGTAAAATTATAAATATTATCAATTTACAAAAGGAGATTAAATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAAGCTTCATCAACAAATAATCAAGTTGTAAGAAAACACGATTTTCAATTTTCTGTTGAAGTAAAACCCGGTTCAATAACCAACCAAAAACATTCAGGTAGATGTTGAATTTTTGCAGAACTAAATGCAGCAAGAGCAAAGGTTATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTAGAAAAAGCAAATGCATATTTAACATGAATTGCAACAACTGGTTTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAATCCTATTTCAGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAAACGATATGGTAGAACAAATTAATTGAAGACTTAAAGCATATACATTCAGAATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTAGAAGCAATTCAACAACATGCTTTGGAAGACGTTTACAACATTTTAGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGACAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTACCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCAATTAAACAATTGCAATCTGGTCAATCAATTTGATTTGGTTGTGATGTTACAACTTTTTATTCAAAAGAAGGAATTTTTGACACTAAATTATATGACTATGATTTAACATTAACAGAAACTCCAAAAGAATTTAATAAGAAGGAAAAATTCGAATCTCGTGCTTCAGTTATTAGCCATGCCATGAACTTAATTGGTGTTAATTTAGATGAAAAAGGAAACCCAATTAATTGAAAAGTTGAAAATAGTTGAGGAACTGAAGTTGGTAAAAAAGGCTATTGATCAATGAGCGATTCTTGATTTGAAGAATATGTTTACATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGTGAAGAATAATAAGTTTAAAAAAAGTTGCACATTGCGACTTTTTTTATTAAAAATAATAATTTATATAATTATTAACTTAATACATTTATAATTAATACATTAATGTATTAGAAAGAGGAAAAATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTCTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACTAGATGAGCAATTAAAGAGGCAATCTTTAAAGCCGACAATCAATATTATTCATTCAACCAAATAAATATAACAAAAGATGAAGTTGGACGCTACGTTTTTAAAGATTTTGAAATTTCAACCTCTAATGAAGGAATATACATAATTGCAGTAGTTTTTAAAAGATAGAACAAATAGTAGGAGTTATTTATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGTGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTTAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTCCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGATCAGCATTATGAAACCATAGAGATGGTTCAAAACAAAGTAAATTGGTAAATTTCGTTGCAAGAAAAGAAATAACAAAAAAGAAAATGGTTAAAAGAATAGCAGATTTAGGTCAAACTTATTATATTGATACTCAAAACCCTAGAGAAGCATTAGCAACCTTGAAAGATTTTGGCCAATTTATTAAAAGAAATAAATCATGCGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGTGAATTTCATAGTGGCGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAACGCATTGGATTGAAATAGAAAAGAAAAATTAGAAGTTAAGGTGACATTTCATAAGCCAATGAAGCCAATTCAATTTCAATCACTTGATAGCCGCGTATTAGCAGAACAAGTTAAATCAATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAATGTAAAAGAAGTTAAGAGATAAAATATGTCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACTAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCGCTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCGCATTTATTACAATTAAAAGCTAGAATGGTATTGCAAGACCCAGAAGTTGAAGAAGAAATTAAACAAGAAAAAAAGAGATTGCTTGAACAGATTGCCGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCAAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCGATTCTTGATGGGCATTCCAATAGTTCAAAATTAGTTGTAACTTTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCCGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTAACCTTTAAAGATGTTTTTTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCGGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGCGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTAAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTTAAAACATACTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGTGTCAAAGATTATATTAGCGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCTGCAATTGAAGTTGCGGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGCGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAGGTTGGAGTTGCTCAAACTCCAGGCAACCCCATTTTATATGGAATTACTAATAAATTCTATGATTATTTCAAAATAAAGACCTTAGGAGAATTGCCTCCTTTTCCTGAATTTAATAAATATAGCAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACCAGAAACGGACAATGATGGAGTTTTAATTTCTAATGAGGGGGCAGAATATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATACTTGGTAAAGGTTTGCGATAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATACACGGATAAATGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACTTCTAAACATATTGACAACAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATTGACAAGGCACCAAATATTGCAATGCATTCTGAAGAAAATTGTCTAGATGATCAAGTTTTAAAATATTTAAATTTTAAAAAAGTTTCTTCGTTTAGACCTGCTTCAATTGGCAGAATAGATAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAACAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATGTTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGAGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGTAAAAAGCATCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTACATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGATAAGAACGATTAAGGAGTTAACATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTGCTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCATATCAATGAATCACCCATTTGTTTCGAACAACTAAGAAGTGATGAACCGGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTATGTAATTATGAAGAAGGTAATAAATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCCTTTGTTTTTGAGGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAGGACAATTATGCAGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTCGCAAGAACAAATTTGGACGAATTTGGTATGGGCGGTAGTGGAGAATATTCATGCAACGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGAGGTTCTTCATCAGGTGCCGCTGCAACTTTTTCTAAAAATATCTCGTTTGCAATTGGATCGGATACAGGCGATTCAGTTAGAAAACCCGCATCAAATGTTGGCGTGGTTGGATTTAAACCAAGTTATGGCGCCATAAGCCGTTATGGATTGTCTGCTTTTGCTTCATCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAACTCTATCATCAGTATTGTTTAAGCAAGATTTAAAACATGATTTGACATCAGTTGATTTGTCGTTTAATGCAGATAGTGTTATTGAAACAAAACCACACAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTTGTAAAAATTTCAATAAATGAAAAGTTGCTGCAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCTTCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACTGGCAATTTTTGAGAAGAAACATTTAATTTATCAAGGACAGAAGGTATTGGAAAAATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTAAGTGATTATTTCACTAATGTTCATAAAAGCGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATCATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACAGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTGGCAATTGACGGGGAAATATATAAAGATTGCAAGATGCTAAGTCATGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAATGATTGAGAAATAGTTATAGGAATTGAAATTCATATTGAGTTAAATACCAAGACAAAAATGTTTTCTCCAATTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCTGGAAGTTTGCCTCTTTTAAATTCTAGTGCCATAATTAAGGCAATTAAATTAGCCAAGGCGCTAGAAATGGAAATAGATCCATTAGTTAGATTTGACAGGAAAAATTATTTTTATCCCGATTTAACAAAAGGATATCAAATTACTCAACAATTTCATCCGATTGGTAAAAATGGTTTGATAAGGGCAAAGGTTCAAAATTCATGAAAAACTTTTTCTATTGAAAGAATTCATATGGAAGAAGATACTGCTAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTTCCTTTAATTGAAATAGTTTCTAATCCTGTAATGCACAGCGCAGAGGATGCGATGGCTTACGTTGAAGCTATAAGACAAACAGCATTAGTTTTAGATATTAGTGATGCAAAATTAAATGAAGGAAGTTTAAGAACTGACGTTAATTTATCAGTAAGAAGAAAGGGAGAAAATGTCCTTAATACTCGCACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCAAAAGGTGATGCAATTAGTTACAAATATTTTCCAGATCCGAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATCAAAAAGAATTAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTGTTGAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCAAGAAAGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAAAAAATGAAATCCAAATAAGCTACCTACGGGTTGAAAAGTTGATGAAGATCAAGCAAATAGAAAACCAATAACTATTTCTTCAACCCAAATTATTGCAAGAAAAAAACGAAACAATATTTTAGAAAAAATTCTTAAGTGAATTATTTATGGAATTATTTTTATAGCAATACCTAAATTTATTAAAAGTTCAAAGACAAAAGGAAAAGAAATTGTAAATTCTGCATATGAAAAAATAAATTCAAATGAATTTTCTTTTGTCCCCGCCGGATATGCAATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGCTCGGTTGATGAAAAGTATGAAGTGGTGCTAAAGAATGTAATTTTGGGTCAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACGTTATGAGGTTCTACAGGCAATGCAATTGCTTTAATCTTATTTGCACTATCAGTATTAATTTTAGCATCGAAGGGATATTCAAAATTTATTTTTTCAATAGATGCTTTATATGAACACAAATCTCCATTCAGAGTGTGAAAATCGAGAATTAAAGGATTCATAGTAAGTGTCTTTGTCTGCTTGATATTGTCTTTAATTTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGACTTGGAAAATTTGATGCCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGATTTACAACAATTTTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCTGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCATTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTACATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGAAAAAAACAAAAAATTTCAATTTAAAGTCATTTGATTTTTTCGCTAATTTTTCATTATTTATAGTTATTAACGACATTTAAAATATAATTATTATAATAATTAATAAAGCAAAGGAAATATATGAATAAATTACCCGGAGGGTTAAGACACTATATTTATTGAGCTCGGTCACAGACAAATATTTCATATCGTAAATGAAACTCAAAACGTATTGCGTTTGTGGGTGTTTTGATTGCTATCTCGGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCCATAAGTGCCTTACCAAGTATAAAATTTAGCTTCATTGGTTTGCCGATAAAAATAACTGGTTTTATTTTTGGACCAATTGTTGGTATGATAACTGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGGTTTGTTCCTGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGTAACTCTGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAGCCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTGGGTATTCAAGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTTAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTTTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATTTTATTGGCGTGAGCAGATACTTCTACATTAAAGACAGATTTCTGGGTTAATTTTACTGCTCAAAGTCTCTTTAGCCCCGTTAAAATATTTTTTAATTTAGCAATAATATTAGCAACGTATAAAGTTGTTGCACCTTTAATTAAATCAAAAGAAGGTGGACATTTATAATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACCAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACACTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAGTGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGCTACATTGAATTTCAAACAAATTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGCTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACTGAAGAGTTTGTTTCTCAATGAAAACCAGTTTTTCAAGACTATAAATTATTGCTTCAAAGTGAATTTGCCAAATTGCAATATGGTGTTGAAATTGAACTTGGAAGTAAATTAGACGACTTATTAAATGAATTAAATCAATTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTCTTTTTAAGAGATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCAACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAGATTTATAAGCCTTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAATTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATCTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCATTTATTCACTTTTATAAAAATCTATATTTTAATGGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCGTTTGAATTATTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCAAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATGTTACAAGAGAAATTCAAACGTCTAATAACTACGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACAGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGAAATTATAAAACTGAAGGGAATGCCATTTTAAAGGAAACAAAAAAAGTTTTAAGAAATTTTAATAAATATTCAAAAATAAACTCACATAAATTAATCAAATTAAAACTAAAAATTTTTAATTTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTACAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGCGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCAAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATTAATAATCCTTCACTTATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGTGAAACATTGAATCAATTGAATAAATTTGTTTTAGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGGAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTACAAAGACGAGAAGACTCAAGAGGATTTAAGCTTTTATATGTCTCAAGAAGACAATATGGAAAATATATACAAATATGAAATAGAGCCATACCACTTTGTTTGATGCACATGAAGTGAATTAAACAGGTGAATAAACGCCAATAATGTTAAAAACAAAGAGCTAATGAATTTATTTATTCCACAAAATACTTTTCAAAGTAAAGGAAATAAAACAATAGACAATAAGGAACCATTCGAAGAAACAACGCTTATAAATTTAATCGATGATGAAGATAACGAACTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGACAGATGCAAAAAATGAAATGATATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTCACCATTATTTTGCCGCCCCCAAATGTAACTGGCAATCTCCATATTGGCCATGCCCTAGACAATTATATTTCTGATACAATAATAAGGTTTAAGAAGTTACAGGGATATGATGTATTGTGACTACCAGGAACAGATCATGCAGGTATAGCAACTCAAGCAGTAGTTGAAAAGAAACTAGCTTCCCAAAATATCAATAAATATCAATTAGGAAGAGAAGAATTTATTAAAAAGGTTTGAGAATGAGTAGATGAATATTCAAAAAATATTCATAACCAATGACAAAAATTAGGTTTGGCCCTTGATTATGGTTTTGAAAGATTCACTTTAGATGATAATTCTAATGAAGCTGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAAAAAGCAATAAATTGAGATCCTGTATTAGAAACAGCTCTTTCTAATATAGAAGTAGTACCAAAAGAAACAAAGCAAAAGATGTACTACATAAAATATCCAATTAAGAATTCAAAAAACTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTCTCAGATGTAGCTTTAGCAATAAACCCGCAAGATAGTAGAGCAAAACAATTAATAAAAGAAATAATAATACATCCATTAACAAATAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTTCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAATGATTTAGCAGGTGAATTTAAGGGATTAGATCGCTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGATATAGTGAAAGATCAAAGGCCCCAATTGAAATTTTAGTTCAGCCACAATGATTTGTTAAGATGTCAGAATTTTCAAAGATGCTTTTAAAAGATTTACGCAGTGACGAAGGAGTAAAAATTTTTCCACCACGTTTTGAAAGTACACTAGAAAATTGAATGGAAAATGCTCACGATTGAACAATTTCAAGACAAATTTGGTGAGGGCATCGGATTCCTGCTTGGTACAATGAACAAAATGAAATTAAAGTTCAAATTCAAAAGCCTGATGGAAATTGAAAACAAGACGATGATGTTTTAGATACTTGATTTAGTTCGGGACTTGCACCATTTGTATTTTTAGGATGGCCACAAGACAAGAACAAATTAAAACGTTATTTTCCTACAAGCTTATTAGTAACTGGATATGATATTATTTTCTTTTGAGTAGCAAGAATGTATTTCTTTAGCTTGTATTTTATGGATCAAAAACCTTTTGAACATCTCTTATTGCATGGATTAGTTAGAGATTCACAAGGAAGAAAAATGTCTAAATCGCTTGGCAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGTACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGTGCTAAACTATTTGCTAATAAATTCTGAAATATAGCTAGATTTGTTGAAAATATTCCTGTTTCGATAACTGAAAAGATTGATTTTAATAAGTTGGATGAATATGATAATTGAATTTTGAATGAATATTTAACATTCAAAAAAAATATTGAAGATGCCATGGGTAAATATGAGTTAACTATTATTTATAAGCATATTCAAGATTTTGTTGTTGACAAATTCTCAAATTGATATTTAGAATTTTTAAAATTTAAAGAAAATTCATATTTAATACATTATTTATTCAAACAAATATTGATTTTATTGCATCCATTTATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTATATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCTTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATACAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCGGTATAGATTTAAAAATAATTAACAAATTAAGCAATTTTTCTTGGAAAGAAAATCATGACTTTTCACTAAAAACAGAAAGTTTTGAAGTATTTATAATTCAAAGCAAGCAAGACAAGGAAAATGAAATTCTAGAATTAAATAAATTAATTGAATTTAGTCAAAAAGAAATTGAATTCAATGAAAAATTAATTAATAATCCTAAATTTATGGAAAAGGCTAATAAAAATACAATCAATGAAAAATTAGCAAATTTAAAATTACATAAAGAAAAATTAGAGTTTTATAAAAACCAACTTAAAGAAAAACAAGAAAAAATAAATTAATTAAAAACAAGATAAGAGTTTTTTAATAAACAATTATCTTGTTTTATTTTTTATTGTCATAAAATTACATTAAAAAATTATTATTTGTCTTGAATTGCTTCTATTCCAGGTAATTTCTTTCCTTGGACAAATTCTATTGATGCTCCACCGCCAGTTGAAATAAAACTAAATTTATCTTGATAACCTAATTTGTTTATTGCTGTAACAGAGTCGCCTCCGCCAACTACTGAATAGCAATCTCTATTTTGAGCAATAGCGGTTGCAATTCCTTTTGTTCCAGCTTCAAAGTTACTGAATTCAGTCACTCCTGCTGGGCCATTTCAAAAGATAGTTTTAGCATTTTTAATAATACTTGCGAATAATTTAATACTTTCTGGTCCAATATCTAATCCCATATTTCCGTCAGGAATATCAGGATTTTCTGGACTTGTAAATTTAGGAGTTTTGTCAGCAAATTCATCTACATAAGCATTGTCAATTGGCAAAACAATTTTATCTTTATATTTGTTTAAATATTCTTTTGCCATTTCAACTTTATCTTCTTCAAAAATAGAAGTTCCAATTTTTCTATTCAAGGCTTTTTGGAATGTATATGCCATACCACCAACAATTAAAACCTTATCTGCTAGTTCAAATAACTTTTCTAGAACTTTGATTTTATCACTTACTTTTGCTCCACCAATTATTGCAACAAAAGGACGTTTAAATCCTACTAATAATTTTTGAAGGTGTTCAACTTCATTATTCATTAAAAATCCTAATGCTGATTCCTTAGAATATTGTGAAATTCCATATGTTGATGCATGTGCTCTATGCGCGGTTCCAAAAGCATCATTAACATACACATCGCCTAGACTTGCTCAATATTTTGCAAGATCTTCATCATTATTGGATTCTGCTTTATTATTTAAATCTTGAAAACGAGTATTTTCTAAAACCAAAATTTCTCCAGGTTTTAAGTTTTTAGCAGCATTTGTAACTGCTTCACTTCTATTTTCGTTAATAAATTTAACGTCTCTATTTAATAATTGCGCCAATTTTTTAGCTACAATTTCTAAAGATTTAGTTTTCTTGTCTTCTTCTGTTTTAACTCTTCCTAAATGAGATAGAATGATTAAAGAAGCATTTTTACTCAATATGTATTTGATTGTTGGCAATTCTTCATCAATTCTTTTTGTGTCCATAATGGTGTTGTTTGAAATAGGAACGTTAAAATCAACTCTTAAAATAACCTTTTTGTTTTCTAATTGAAGGTCTTCAATGGTTTTCTTCATTTTATCTCCTTGTGTGTCTTGTATGTTTTATATAAATAAAAATAATATTTTGTTTAAATTTATATAATTATAAAGAAAAAAAGTTATTTTAAAATCAACTTGGGAGATTAAATGGCAAAAATTTTAAATGCTCAAAAGCAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTTCATTATTTTAAAGAATCTAGATGAATGAAAACAATGGATGAAAAATTGCAAGATCAAGTGCAAGAATTTTCAAAAAGGCAAAAAAAATTAAATGCCAAATATAAAAGCTACATTGAATATTCTAAATGGTCTGAATCGCCATATGCAACAAAAACTAAACCGCTTACAAAATGAAATAGAATCATTATTATTTCGGTTTTCATTATTTTAGTAATTATCATGTTGCTAATCGTAATTGGCTTGAATAAATTGTGATAAAAACGGAGGTATTGACATGGAAATAAAACTATTAATTGTTTTACTAGTGTTGCTAGCAGGTGTTATTATTTTCATAATTGCTAATTATTCAATTACTTGAATTAAAAACAAAAAAAATTCAAAAAGTAACAAAAAGGTTTTGGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCCCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATTACTATTGAAAACAAAGATAATCTTGATTTGCTAAGAACTGATGGTGTAATATTAAATGCTAACATTTCTGCTTCTTTAATTATTTCTATTTTTAATAAACACGCTCCACTTCACGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGTGCAAGACACCGTGCTGCTATGGGTATTTCAGAAATTTGCGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGCGGAATTACAATAGCTAAAAACGGAACTTTTAGCCCTGTTTTACTAGAAAATCTACAAGAAAGATTAGTCAATATTTTTAAGGATAATAATTAAAAAAATAAAAATTTTGAAATTATTAAAAAAGTATGGTAAAATGTATTTCGCAATTTGAAAAAAGCGATCGTTTAATTGCTCAAATTGCCGCTTAAAAAAATAAATATTATAGTTATATAATATACTCTCGCTCTTTTCTTATATTTATAATAAACCCTTATTTATTTTATACACATATACATACAACCCTTATTTTTTATATTTCCTTAACTTAGAACTTGAAGTTCAACTTATTATTAAATTAAATAATCAAAAGCTTATATTGTTCGATTTTTAGCGAGAGTAATCTTATTTTAAAAAATATTTCTTTCAAAATAGTAAACAAAAATGTTTGCTATTTTTTAATATTTTTGTTTAAAATATATAAGTACATAAATATCACTATTTTATGGCCAAAATTAAATCATTTACATTAAAAAAATATTTTTTTTGTTAAAATTACATTGCTATAAATGCGCGCTTTTGGACAGGACATAGTTGAAAGGCTATGCTATGGTAGGTTAAACGCGTTTAATCAAAAGATTTAATAAATATTAAAAAAATTAAAATGTAATTTATTGAAAGGAAATTATTATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTACGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCTGACTATGTTAAAAACATGATTACCGGTGCCGCCCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATAGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGATGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGATGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATACGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATTACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTAAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACACACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTGACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGGAGAACTGTTGGTGCCGGCTCAGTTACAAAAATTCTTAAATAATTATCAAAATTACTAAAAGAAATTTTGAATTACAAAATTGGATTTGTATAAATCAAGTCCAATTTTTTTATATTTTATTTAGTATGTTTTGATTGCAAATATTGTCATTTTTGTTTTTTAATATTAATTGTTAATAAAAAAAGTAACCATTTGTTCTTTAAAAAATGCCCCCCCCCCTATTTTTTAATTATTTTATTAACTTTTTAGTTAATAAAAATAAAATTAATTGATTAAAAAAAATAACTAAATTTCCATTTTTAATATAGGAGAACGAATGAATGAAAAAAAGAAAAAAATTGCCATTCCATTGGCAATTTTGTGCGGCGGTTTAGCAATTGCTACAACAGCGCTTATTGCAATAAAGGCTCGCCGTCATAAAATTGCTAATCAATTGCAAAAAGAAAATTTATTGCAAAATTTTAAAAAATTACAAAAGCAATTAAACGAATTGCTAGGATATAAAATTGTTAATGAAATAAATGTTTTTCATGAACAAGAAGTTTTGCAAGGATCACTAAAAATTAATAATAAATCTGAAACCAAAGTAATTGAAGAAGAAACTTTAAGGTTAAAAGATGCTATTACTTTATTAATATCAAAAATAAAAAATCAAATAAATCAAAAAGAATTGGAATTTGCTAAATTTAATGAAATAAAAGATAAATTGCAAGAATATATCAAAAACGAGTTATCGAAACAAGAATACGAACATATTAAACAAAATATCGAAAATGAATTAAATAAATATACTCCAATTAGTCTTGAATCAACTTTAATCGAAATACAAAATGCAACAAATAATCTAATTAAATTATTAAATGAATCAACCAAAGAAAAAGATAATATTGATAATTTAAATGCCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAAGCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCAAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAACGCATTAGATTGTTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGATAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGATAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACCGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAGATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGGTAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTGCTGCAAACAATGAATTAAAACAAGCCCTTGAAGTGGCAAAAGCAGCAAAAGTTAATGCTGATAGTACAAATAATTCAATCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAAAAATCATTAGATGCTGAAGTTAAAAATGCAAATCAAGCAGTTACTTCAAATAATACTGCTTCAATGCAATCAGCTAAATCTTCATTAGATACAAAGATTAATCAAATCAATCAACAATTGCAACAATTTAACAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAAAGACATTGATAGTTTCTTAACTGAAGATGTAAAAAGAAATCCAAATTACACAGCATTAGTTAATACTTTAACTTCTGCTAAAACAGCTAAACAATCAATAAATAAATCTTCAAATAAATCAGACATTATTACCGCAAATCAAGCATTACAACAAGCATTAAATACCGCAAAGGCTAAAAAATCAAGTATAGATAATGAATTAAGGCCTCTAAAGAATGATTTGCAAAGTAAAATTGATGAGTTTGGACCTATAAGAAATACTAATTTTTCATGAATATCTAGCAAGTTAGAAACTGCTAAAAATAAATTAGCAGAAGAATTAACAAAAGCAGATGCAATTAAAAATAATCCATCAAGTTCTAAACAAGCATTAAAAGATTCTTCACAACAAGTTCAAAAATTAGGAAATGAATTATTGAAGACTATAACCGAAGAATTAGGAAAGGTTGAAACTAAAAATAGTAACATCGGATATAGATTGTTTAAATTGGCACAAGCTGAACAATTTAATAACACTGATGTTGATAAATTAAAGAACGCTTGAGAAGAAAAACAAACTCTACTTTCAAAGAAACAAAAACAAGGCAATCAATCAACAAAAGATTATTTAACCCAATTATCTACAGAAATGAGCACTCAAGAATCTACAATTAAAAAAGTTATAGTGGATATTCAAGCACATATAAGAAATAACCTTAACATACAGCATAGATCAGGAGCTCAAAAGCTTATTGACAATATGAAGACATTTTATGGAGATAAAGTTGGTATAGAAAGCCTACAAAAATGACAAGATCTTATGGATGATAGTGTATTAAATGTAGATGATACTTTGAAAGACGACTTTAACAAAGCACTTAGGGTATTAGTTGGAGATTACACTAAAAACCCTCCTGTTTCATCGTGATTTATTAATAAAAGAAATAGAAGTATAGAAAATTATCAAAATCTAATAAATTTAATTTTGGTAAGAGAAAATGAAATTTTATTAGATAAAGCAAAAGACTTAGATAAAAGAGCAGAAAAAACAATTAAATTTGTTGATGAAAATATTAATAGCCTTGACCAAAGAGCAAAAAGATTAAAACAAGAAATATTAAATGCTAAAAATGACTTAAGTAACTTTACACTAAATCATCAAAAAAACCAATTTACTGCAAAAGAAATAAACCCTAAAATTTCTCTATTAGAAAATAAATTAAATGAAATAAATCAATATCTTCTTCCAATAATAAGAGAAAAAGCAAAATCAAAAATATCGGAAATTGAAAAAAATAAAAAAGAACTTGAAGATATTATAAGAAGCAATTTTTATCTTTGAGAAAAAAATGAAATTAATAAATATATTAGTGAATTAACAAACAAACAAGTAGAATTAAGATCTAATATAAATTTCGAAAATAAATGAGAATCTATAAAAGATACATTAGATAACTTAAATATTAAAGAAAATGTTTCTTTATTAAAAGAAGTAATAATAAATAATTCAAATGCTCAATATTCAATTAATAAAATATTGTTAACAGTTCCTGAATTTATTAAAGTTGTGCAAACAACTAGAAGCAATAACTTGCGGTCACTGTCAGAACAATTTAAATTTGTATTGTTAGACATTAATAAGTATTTAAAAGAAGTAAAGAAAATACTAGACGAAAATAAAACATTAAATAAGGATAACATAACTAAATTAAACGAAAAAATAAGATTGTTTGTGGACAAATCCCGTGAATTAAAAGAATCATTAAGATGATTTGACAGGCAAAATGAAAATATAAGTGATCAAACGGTTAAAAACAAGTTGGATGAAGTCCGTGAACTTCTTAAAAACGTTTAGTTTTGTAAGGTCTTTAAATTAAATTTAAAATTAGTGCAAAAAGCACTAATTTTTTATTTTAATGTTAATCAAAAAAATAATTTCGGATTTTATTTTAAAAAAACTTTATTTTTCTAATGTGTTATAATAATAAATTAAATGCTTATTTTTAAGTATTTATATTGCAAGGAAAGGAAAATATGATAAAGAAACGAAGAAGAAAATTAGGATGATTGACCTTTATTGCTGGGGGAGTTGCCATTACTGCTTTTGTTTTAATAATGTGTAGATTTTGCAAGAAACCTAATAAGCAAATAAAAGATCGAACCTCTTTAAATTTATTTAATAATGCGCAAAGTGAATTGAAAGAATTAATTAATTCTCCAATAGCCAAACTATGCCAAACCGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGCTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCTAAACAAGAATTAGAAAAATTCAACATGGCTATATTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTATAGATACCAAAAATTCAATCAATGCTTTAAATAATGCTAAAAAATTGCCTTTTAATTCTATTGAACAAGTTAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCAGAACCTTCTAAATATGTTGATAGTTCTAGTGAATTACAAACATTTAATGATTCAAATGTTGAAGCAAAATCAACACTTGAAGATATTGATAAAAAGATTGATGCCGCAATAAGAGCAAACAATGATTTAAATGAAAAAATTCAAAATTATAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAGATAAAATTAATGAAAAGATTAAAGAAGTAAAGGGCGTTGGAAAAAATTCGACAATCAAAGAATTACAAGTAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAATAAAGTTAGAGAAATTAGAAAATCTAGAATATTATTAGTTGATGCTGAATATATTGCAAGATTTTTAAAATCACAAAATGGTGAACGTTTTAGACAAGAAAATGAATTAGGCGAATTTCTCGGAACAAAATTTTTAAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAGGATTTAAATTCTCTTGATAGTGTTGATTATAAAGTAATATGAAAAGCAAATAAGGAATTAGAAGAATTTATTCCAATAATTAATGGTGCAATTAAGCAATTTGTATATGACAATTTTAAAAATTGGTCTGATGTGTTTTCTATTAACAATTTAAATAGTTGAGCTGAATACTGCTATCCGCCAAAATATACTGGCGCAGGTGAAAAATTAGTAGCATTTAAAAATGAAGTTCAACAAAAAAATGAAGAACTAAGAGCCAAGGCAAAAGATGCCAACTGAATGGATATTTTAACAATTTATAATCCACAATCATGAAAGCTTAACACTAGTGACATTCGTGATAAATTTAGAGAAGTTTATATAACTGAAGAAGCAAAGAAAAATAATCTTGCATATCAAGCTGCTGATTTATTAAACAAATTAGAATCATTTGATTTGAACAATAGCAAATTAAATAAACAAGAAATTAAGAATTTTAAAGAAAAATTAATTGCAAAAAATCTAGAAATTGAAGAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGTATATATAACCCTAGTCTATGAATGCGTGATTTGGATTGAAAAATAAAAGAACAAGAAAAAACTTTAAAAGATATAAATTCTAAGATTTCTCCAATTGAAGATAGTTCATTTACAAATCAAGAATTAAAGAATACTTTCGTTGAATTAAAACAAAAATTATCAATATTGAATAAAAACTAAAGGGTTAGAGTCCTTTTGGGTTAAAAAAAGTATATTAAAAATAAGGCTTCATAAGTGCCTTATTTTTAATTAGTTTAGATTATTGAGCATCTTTTAGTTCGTTTAAAATATCATCTAATTCGCGGATATATTGAGATGCAAAAATTTCTTTTTCATAAAACATAGGAAATTTTAATAGGAACAGTGTTATCTTCATTGTCTTTTTTAACTTTATAAAATTTTCAATTATAGTAGATATTTTCTCAATTTGTTTCATCTGAAAATATAAGTTTATAAGAAATATTTTTTGAAAGCAATTTTTGTATTTTTTATTTTAATAAAAATTTTATTTACTTATTTTTTAGTAATTCCTTTTGTTTTTGACCCATTATGTGGCACTTTCTGCATCTTGCTTCATATTCATTATAGTCACCAATTAAGTTTTGCTCATTTGAAGAATCTTTTCTAAAACTTGTTGAAGCAGCATGTTGACATACTACACAAATTGCTTGCAATTTAGTTACTCGTTCAGCCATTGCCATTAAGGCAGGAATTGGCCCAAAGGGTTTTCTTAGATAATCTTGGTCTAGGCCAGCTACAATTACTAAATATCCCTTATTTGCTAAGTCATCAGCGACTTCAATTAATGAATTTCCAAAAAAATGGGCTTCGTCAATTATTATTGCCTTATATTTATCTTGTTTAAGTAATTCATAAACTTCGTTAATGTTTTTTAATGAATGTGTTTTTCTTCTTACACCACAACGGCTTACTATTTCATCATCAGAAAAGCGATTATCATATTCGGGCTTTATTAAAAGCGGTAGCATCTTAGCATATTCTAAAGTTCTAACTCTCCTTAATAATTCTTCACTTTTTCCACTAAACATTGGTCCAGTTATAACTTCTATCATTCCATCATTAAATGTTTTATACATTTTTATACCTCAAACTTTTAAATAATAATGTTTTAACATTATAATATAATTATTACTTTTTTAAAGGAGTTTTTATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACGTTATTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAGCCTAACGTTGCAATAGTTAATTTAGAAGATCCAAGACTTTGTGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCCGGATTAGTTGCTGGCGCTTCAAAAGGAGAAGGCTTAGGTAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAATAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATTAATAGAATTGGCAAAAAAGCTCAAAATTCAGGTGATAAAGAATTGAAATTCGAATTTGAAGTTGCCTTAAAAATACGTAATGCATTAGAAAATGAAATTATGGTTAAAAACATAGATTTGAGTCCAGAAGAACAAGTCGTGATCAAATCATATCAATTGCTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGTGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTATGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCGGGTAAAATCGAAGCAAGGGCATGAGTATTTAAAAAAGGAATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTTGTAAAAGCAGAAGTCATTAAATATGAAGATTATGTTGCTTTAAATGGCGAAAAAGGTTGCAGGGAAGCCGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAAAAATTAAAATTAGGGGAAAATACCTAATTTTTTTTGTTGTATTTTTTTAAAATCGTATAATAAAAGAGTAATGATACTTATCATAATGATATGATATGAATTAACGAAATATAAAACTTGACCAAATTTATTTGCTATGGTTTTAAAGTTAATTGCATGATTAAAAAGTTTATTGATATACTATCAAAACTAATACTCATTAAAAGTTTCATTACATCATAAAAATTTTATTAGTCTATATATATTGCTATATAGTATTTTTTGGTATTACATTAAGTTATTTGTAGGAAACGAACTTTTAAATTTCAACCTATTTTAAGGAACAATTATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCTCAAGAATCTGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAATTGCTAGTTTTTCTGATCATGATAATTTTTATATTGAAAGTTATTTGAAACGCCTTGAAATAATCAAACAATACAATATTGATATTACATTATGACCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATTGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAATAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGGCAAGATATATGCAATAGCCAAGTTGATGGGCTGGATATAACAGATTTTAATAATAGCAACAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGAAAAAAATATCCTCAATATGGTAAATTTTGTTCTTATGTGGAGGTTGAAAATAAAGATCAAATTACATTTAATGAAGTAAAAAACAATATAAGAAATAATAAAGTTGATTATGACAAAGTATAGAAAGGAATAATATGATAAAAAGCCTAAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAGTCAAGTTAATGTTATTGTTGGTGAAAAAGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAACAAAAAATTATTCAAAAACGAATATGAATGATTCAATGAAAAGGCTAAATTTTTAGTCGATTCAATAAATATTGATAATACAGAAATTAAAACACAGGATTTTTCATATATTTTTGATTCGGAAAATAAGCAAAATTCAAAAGATGCAGGAATTTTAGAAATTCAAAAAAACCTAGGCGGTTATATTAGCCAAAATGATAGCAGGAAAAATTCTCTTGATTCTTCTGAATATGTTGAAAAACAAAAAAGTAATTGTATTGATAATTTCACAGAAAAAATTTACTATGATCAAGATCAAAAAATGTTAAAACAATTAGACGATTTTAAATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGACAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAGAGAAAAAATAAATAGTACAAATTTGGTTGTGAATAATCTTTTGTCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTGGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTCATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAAGATAAAAAAACTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTGAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGACAATATAATTCAATAATTGAAAAAGAAATCATTTTAAACTGAACATTAGAAGAAATTGATAAAAAAAATAAGAATATAAAATATAAAATAGAAAGTTTTGCTTTAAGTGATGACGAAAAAAATTTAATTTTGAATAAATGACTCGGTTCAACAAATAAGAGTAAGACAATCGCTGATGTTTTTAATGGTAATATTAAAGATAACTTTGATATTAAAAAGATGATTAAGACATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGATACATTGAACAATGTTCCTAATGAACAATACTTATTGCTAGACCAAATTGAAGATAATCTAGACAATAAAACAATATATGAAAGAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGGCAAACAAATTTTTATCGTTACACATAACCCTAATATTGGAACATTGATAAAAGGCAATATAATAACAACAGATATTTTTAATACTAATTTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACTAAAGGAGATAAATAATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGAAATAAAGAAAACATTAATAATTTTATAATTAGAGCAATATCTATTAGTGACGAAAAATTAAGTGAACCACAATTAACAGAATCGGCTCAAGAACAATTACAAAAGGGTAATAAACAAATAGAATTTATTAAAAATTTATTCACAAATTTTGTTGAAAGATATAATGAAAATTAATTTTTATTCAATCTTAAATAAAGCACAAGGTTTTTAATAAAAACATTTATTTTTAAGTTGAAAATTAAAAAAACAAGGCCATGAAGGCTTTGTTTTTTTAAAAATTTTACATTTTTTCAGGTGCTTCAATGTCCATTAATTTTAAACCATCAGCTAAAACAATTTTTGTCGCCTTTACTAAGGCTGCAAGGCTTTGTTCGTTAGGTGATCCTAAAACTTTGGTATTTGAATAAAATGAGTTAAATGCCTTTGATAATTTTAATAGATAAGGAGGCAACAAGTTGACTTTATAAGTTTTGGCCATTGTCGAAATTAATTCGGGGTATTCTTTTAATTCTGCAACAAGCTTTTGAGCATATTCATTATCCAACTCATCAGAAGCAATAGGGTTTTCAATTCCTACTTTATCTAATAATTGGCAAGCACGAGCATGAGCATATTTAATGATATACATAGGGTTATTTTCGTTGTTGTCAGTAACTTTGTTAATATCAAAGTCAATTCTTGAATTATATGATCTATCAATTAGAAAGTATCTAATTGCATCATTTCCAACTTCTTCTGCTAGTTCTCTTAACGTTAAAGAAGTTCCTAAACGTTTTGACATTTTAACTTCTTTACCATTCTTCATGATTCTAATTAGTTGGAATAGAAGAATTTCAATTTGGTCTCTTCTATGTCCTAAATCTTCAAATGAGAATTTAACACGTTCTACGTATCCTGAGTGGTCAGCTCCTCAAATGTCAACAATTTTTCCATTTGGATCATTTAATTCCAAAAGTTTTTGTTCGTGGTATGCAATGTCCGCACAGAAGTATGTTGAATCACCATTGCTTTTGATAATAACACGGTCCTTGTCATCTTTGCCACCCTTTGTTGTTTCAAGTCAAGTTGCGCCATCTTTTTTGTATGTTGTCTTTAATCTATGAATTGCTGGTCATACTCTATTTTTGGCATATTGTTCTGCTTCTGATGTGAAAATATCGAATGTTACTCCAAATTTTTTCATATCTTCAATAATATTTTTTAAAGCAAAATCTCTTCCGAAACTTCTAAAGATTTTTCTAATTTCATCAGTATATTCTACATTTTTGTATTCATCTTTGTGAATGTTATAAAATGCTTGAGCGAATTCTATCATATCTCCGCCATGGTATGAATCTTCTGGCATAGGAAATTCAGGATTAAATATTTGTTGGTATCTAGCAAATATTGAATCTGCCATTTTATTCATTTGGTTTCCATAATCATTAACTAAATATTCTCGAACAACGCGGTGACCTGCTTTTTCTAGAATATTGCACAATGTTGCACCAATAGCAGCATTTCTTGCATGTCCAATATGCAAATATCCTGTTGGGTTAGCAGACACAAATTCAACATTAATTGCTCCTTTACCTTTTGGATCAATACTTCCATATGTTGATCCTTCGTTGTTGATTTGATTTATTGTGTTGGCTATAACTGAGTTTGAAACTCAAAAATTAATAAAACCAGGTTCGGCAACCTTTATTTCACTAATTTCTTTAAATTTATTTAAATCAATATTTCTTACTATTTCTTGGGCAATATCTATTGCCTTCTTACCTAAATCCTTTTGCAATGTTAGGGCAATATTGCTTGAATAGTCGCCGTAATTTTTAGATTCAGTAAGTACAATAGGCTTAGAAATATTTAATTTTTCAAGTGCTTCATTTAATGAAGCAATAATAATGTCTTTCGTTGTTTTCATATTCATAATTATAATATAATAAAATTTAAAAACATTATATTTATAGTTATAATATTGAGTATGAAAAGATATTATTTATGTGGCCCAACTGTTTATAACTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTCACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAAACAGAAAAAGAAATTTCAAGTTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAGATTGTAAGAGTTACTGATTCACTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAATGGCAATGTTTATTTTGACGTTTTAAAATTTAAAGATGTGTATGGAAAAATAAGCAACCAGAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAACGCTCACCATATGATTTCGCTTTATGAAAAGACACAACTGAAGGTATAAAATTTCAAAGTCCATTTGGTCTTGGAAGGCCAGGCTGACATACTGAATGTAGTTGTTTTATAAATAAATATTTTGAAGGCAAACAACTTGACATTCATGGAGGAGGTATTGATTTAATATTTCCTCATCACGAAAACGAAAACATTCAACATTTTGCAATCAATGGCCAAGATATTGCCAAGGATTGGATCCACTTTGGTACATTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAAAATTATTCGGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACTGATGAATTACTTGCATCACTTCAAAAACAAATTAATAATATTGAGGTACATTTTAATAAAAGTCAATTGGAAGATATTGAATATACGCTAAACAAACAAACAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCCTTTAAGCAACTTAATGAGCAATTAAAATCACCACTGAATTATGGTAATGCATTTTTTGTATTTAAGGTTTTAGGCTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGAAATATTTTAAAAGAACAAAAACAATACGATAAAGCAGATGAAATAAGAAACATTTTAAAAACAAAAAGGTTGATTTAATGAAAAAAATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAAATTTACTTGCAAAAAGGACTTGTTAACAACAATTTAAAATTTAAAAAGGTTGTTTATTTAGATAGAAATAGTCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGTAGTATTTTTAAAGACGACTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCTCAAAATTTTGGTGCTATTATACGTAGTGCCAATGTGTTTGGCTTTAAACATATAATCATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGTGTTTTAAAAACTAGCTCAGGGGGTTTTAAAAACGTTAAAGTAATTAAAGTTAACAGCTTATTTGATGCAATTGAGGAATTAAAAAACAAAGGTTTTTGAATATATGCATCGGCCTTAAACCAACAAGCAAAAAAACTAAATGACGCAAAATTTAATGAGAAAATTGCAATTATTGTTGGAAACGAAGGGAGTGGCGTTTCAAAAACATTGCTTAAACATTCGGATGAAATTATTTACATTGAGCAAGATAAAGAATCCGTTCAATCTCTTAATGTTTCAGTGGCAACAGGAATTTTATTATATACAGTTTTTAATAAGAAATAAAATAGCAATTTAAAAAAACAGCGGGTCTCGCTGTTTTTTTGTTTATATAGTTTTTTAATATACGCTTAATTTAAAGTAACTTTTGTACCGATTTTTTCTGCTAGAATGTTTTCAAGATCTTTTAGATCACCAATAAATGCTGCTTTTCCGCCTTCTTCAACGAATTTAATAGCTGCTTGTACCTTTGGTAACATTGATCCTGGGGCAAATTGTTTTTCAGCAATGTATTTTTCAAGTTCTGCTTTTGTAGCGTGTTTTAGATCTTTTTGATCTGGCTTGTTGTAATTTACTTTAACAAAGTCAACTGCTGTTAGAACAATAAAGTAATCAGCTTTTGCTAATGCAGCCATTTTTGCTAAAGCGAAATCTTTGTCAATAACACCATCTACACCTTTAATAAAGCCATTTTCATCTTCAACAGTAGGAATTCCACCACCACCACCAACGATAACGGTAGCACCTGATTCAATAGCTTTTTTAATTTGATTAATACCAACAAAGTTTAATGGTTTTGGAGATGCTACAACTTTTCTGTAGCCTCTACCTGCATCTTCAACAATTGTGCTATTTGGGTTTGCAGCAACTGCTTCTGCTTCTGTTGCAAAGAATGGGCCAATTGGTTTTGTAGGATTTTGGAATGCAGGGTCTTTTGGATCTACAAGAGTTTGAGTTAAAATGTATAAAACTTCCTTGTTTTTTAATCCTTCGTTTTTAAATGCATTTGTAATAGCATTAACCATATGATATCCAATGTATCCTTGGCTCATTGCACCTGCTTCAGGCAATGGAACTAAAGGAGACTTATGATTAACTGAGTGAGCACTAACGAAACCATTGAAAATCATTCCAACTTGTGGTCCGTTTCCGTGTCCAACAATAACTTCGTGTCCTGCTTTTACTAATTCAGCAATTTTGGCTGCAGGAACTTTGACAAGTTCTTTTTGTTCTTCGGGGTTATTTCCTAAAGCGTTTCCGCCTAATGCAATAACAATTCTACTCATATTAGTATCCTAAAGTAGCTAGAATAACAGCCTTAATTGTGTGCATACGGTTTCCAGCTTGTTCGATTGATTTGTTGTATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCACCGGTTGAAACAACTGGGTATTTTGCACCTAAGGTTTCTTTAATAGTTGCTGAGAATTGAGTTTGGTCATCGTGGAATGCTGGTAAGCAGTGTAGGAAGATAACATTTTCTTTTGCTGCTTTGATCATTGCCATATCAACTTGGAATGATTGTAATTCTTGAATACGTGGTTCAAATAGTGAGAAGTCTTCTCCTAATGATACTCATACGTCTGTGTAAATAGCATCTGCGTTTTTAGCAGCTTCTAATTTGTTATCTGAAAATGATACAGAACCACCATTACGTTTGAATAGTTCTTGACATGCATCGTAAACAGCCTTGTGGTTTGGTCCATTTTTAACAATTTCTCATTGAGCTTTTGGTCCACATAATACAATATTAACACCAAAGAATGCAGCACCAATCATTAATGAACGAGCAACGTTGTTTTTAATATCACCTGAGAAAACAATTTTCTTTCCTCTTAGGTCTCCAACGTGTTCTTTAAATGTTAAGTAGTCAGCTAGCATTTGTGTTGGGTGTTCAGCATCGGTTAAACCATTTCATACAGGAATTCCTGAGTATTTTACTAATGCATCAACATCTGATTGTTTGAATCCACGGAATTCAATACCATCATAAATTTGGCCTAAAACTTTAGCTGTGTCAATAACTGATTCTTTTTTACCAAAGTTTGATCCTGTTGGGCCAATGTATGTACAGCTTGCACCTAAATCAGCAGCTGCAACTTCAAATGAACAACGAGTTCTTGTTGAGTCTTTTTGGAACATTATAACAATGTTTTTTCCAACTAATGGGCGATTATTTGTGTGTAAACCAGCAAATTTTGCTCTTTTTAGTTCTAGTGATAAATCTAGTAAATAGTTAATTTCTTCGGTTGTAAAGTTTAGAGCGCTGTCTAAACTACGACCCTTTAAATTTAATGGCATATTTTCTCCTTAATTAAAATAAATTTATTTAATAAAAAATAAGTGTGTGTTTTGAATTTTTCAAGTTTTTTAAAACACGTTATAATTATACAATGTTTTAAAAATATAGATTTTTTATTTTTCATTATAATTTTTATAAAAAAAATAAGATTATTTTTCAATAACCTTATCTTTTATTTCTTTTAAAAATGTTTTATTTTAAATCGTTTTTTAAATCTTTTTTATTCCCAGAATTTTTCGTTTCTAATAAATTTTCATTTAATTTTTTTAATTCATTTAAAATTAATTCTTCTGTTGTTGGAATAGGTTCTTCCTTTATTTCTTCTTCAATTGGGTTCTTTTTAGCTTTTGCATATAGAATAGAATTCTTAATTGTAAATACAATCATTAATCCTATAAAAATAAATGTTGAAACAATAATAAATTGAATTAAAGCACCCAAAAATTTACCAATAAAAATTCCACCAGTTTCTTTTGTTGCATGAATGCCAGGTCAAACTACTCAAGCATCAAGATTTTTCATACCGACCGCCTTTGTGATTGCTGACATAATAACATCATTTGCTAGTGATGATACTAGTGCCCCAAATGAAGCTCCTAATAAAAGACCAATAGCTAGCATAAACATGTTTCCACGCGAAACTATTTTTTTTGCATCTATATATGACTTTTTAATATAATGCTTTTTTTCTTCTAATTCTTTACTTGTCATATCAATATTATATAGAACAAAAATAATTTATTTCTTGTTTTCTAACTAACAATAAAAAAATAATAAAAAAATGGCGGGTAAGAGAGGATTTGAACCTCCGCGGGTGTTTTAGCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCACTTGAGTACTTACCCATTTGCGCCATTGCATTAAAATTATATATAAATAATTTAAAAAAATAATAAAAATAAATCAAGGAAATTAATTTTTCCTTGATCTTGGAGGGTAAATTTTATTGATATCATTGCCACTATCAAAAATTCCTAATTTTAAATTTTTAGCATGATTAGAAGCTTCTTGCAATTGCTTGTAGTAGTCAATGTTCTTTGTATAATAATTGCTTTGAGGATCTATGCTAATATAATACATCCTTGCCATTCCAAAGAAAACCAATTGTTCATTTAAATTATAAATTTTTTTAGTTATTTTGTCTTGATAGTAAATAATTGCAACAATTCTTTTATAGTTATCATAAAAATTTTTGTCTTTTGTATTAACCCTTGATTTAGTTTCCTGTGGGACTACTCAAATTTTAGCAGCATTGTTTAGAAAATTCTTTGTATAATTTTCGGCAATTTTTCCATATTTATATTGAATGCCGGATGTTTCTTCGAAATTTCCATTAATCATTTTATGAGTTTCTGGCGTATCAATTCCATTAAAGCGATATTTGTTTCGATAAGAATCTGATATTGTATCTCCATCAGCAATCCTTGCAATATTTATTTCTGTTGCTTTTCCTACTTCAAATTTATTATTTTTTAGTATTTCAAAATCAAATTTAGCTTCATGAAAATTATTGATGTTAAATTTGTCATTATTTGTGTCGTTTTGATTATTTGGCTTAGCTATCTTAGTAAGTTTTTTTATTTTTGATAACAAAATATCAAATTCTTTAATTCAATTAACTTCTTTGCTATTTGAATTTATTAATTTTTTGGCATCAGCAATTAATTGTTTATAATAATCATTTTTTTGCCATTCATTGCTATTTTCAATTTCCTTTATTTTTTTGTCATATTTTTCTTTAAAATTTTGTTGTTCTTCGTCATTAATTTTTTCTAGTTTGCAAGAATATGAAACTATTGAAATTGGCAAAATTGTAGCAATAGAGCCCAATGCAAGATTCAATATTTTTTTAGTTTTTCTTTCCATATTTATTCAATAAGTCCAACGTACATTTATATATTGCTTGATTTGAATTTGCATAAAGGATTCTTGGATTGTAATTTTTGTTTTCCAAAAAATTATTATTTTTTAAAAAATCATTTAGGGCTTTTGCATTGGCGATTTGCAACTCAGTATTGAAATTGATTTTTTTAATGCCTAATCTAATTGCTTTTTTAATATCATCTTCTTTGATCCCACTTGCTCCATGTAAAACTAATGGAATTTTTATTTCTTGATTTAATTTGTCTAATAGTTCAAAATCTAGTCCCTTTCAATTACTTTCATATTGACCATGAATATTTCCAATTGCCACTGCTAATAAATCAATTCCAGTATTTGCTAATTTTTTAGCATCTTCAATGTTTGTTTTTTGAATATCAAGTGATTTATTTTTACTAATATCTAGTCCAATTCTACCTACTTCTGCTTCAACAGTTATATCCATTTTTTTTGCAATGTTAACTATTTTTTTTGTGTTCAATATATTGGTTTCAAAATCTTCTTTGCTACCATCATACATTATTGACTTAAAGCCGGCATTTATAGCATTAATACAATCGTTATAATTACCATGATCTAAGTGCAATATGACATTAGTTTTAATTTCTAAATCTTGAATAAGACCATTAACCAAATTGACAACGGTTTTATAGCCACCCATGTATTTAATTGCAGAAGGAGAAGCTCCGAGTATGATATCTAAATTTGCTTCTTTTGTTGCTAATAATACGAATTTCGCTCATTCTAAATTGTTTATATTTATATGCAAGATTGCATAATTTTCCTCTTTTATTTTTTTAAGTGTTTGATTTAAATTTTGTATTTCCATATTTTTTCTCTTTAATTTTATCATTGCAATAGTCATTTTAAAACAATAAAAGAAATAAAAAAACTAGCATACACTAGTTTTTAAATTCAGGTCTTTTAGAAGTTCAAAGTCCATTTACTTTTTTAAAAAAACGCCCATCTATTGTTAGTAGTTTATATAATTCACCACGTTTTTTTATTAATAGCTCTTCATCAGAAATACTAGATGTTAATTCTTCTTTTCAACGTTCAAGTAGTTCGTCTTTTACATAATTAAATAAAGTTTCAAAATCTAGTGCATCGTGTCTATCTAAAATTTCTTTGGCTATATCAACTTGCGATTTATATTCCATAATTACCTCATAAAATTTGTTTTTTATTATAACACTTTCTTACAATAATCTAATAAGCAATTCTCTATATATTATATAGATATAACTTTCATAATGACAAAAAATGTACATAATGGCGAAATTTTAGTTAACTCTAGTTAGATTTTTTCAAGTAAGTATATTGTATTTTTACTATAGACCATGAAAATATTTTGAATTTTACTTTAGATTCAAAATATGGAAATAAATAATTGTTTTATGTAGAATCTTTATATATTTTAACTTTTTTAACATTAGCCTGCCTAATTTTAAAATATGATGCATTTTATGCATAAAAAATGTTTTAAATTCTTTGCAAATTTATAAACAAAAAAATATAGTATAATTTATTACGTATAAATCATAATTAATGCAATAAAAGTGCATTTGTATTTATACGCTATAACATCTATATTTGAAAGGAATAAAAAATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTTTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGATACTACGACCCAGCTATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATTAATGTTCCTAAATGAACAAACTTAATGCACCTAGATACATGACTAACAATGTTAGACAAGAATAAATTCTTGTACTCACCAATTGCTAACGATGTATTCAAATTCTGAGACTACGATTTAGTTAACGGTGGAGCAGAACCTCAACCACAACTAAATGGATTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTCGATGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTATGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTACCATTCCACGGAAACCAATTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGATAATTTATAAATATTTATAATAATATTTAAATTTATATAAATAATAAACGGACTCACAATAGTGGTCCGTTTATTTTATTAATATACTTTAAATAAAGATTTGTTTTAGATTTGACTAGTTTTAATAATTATTTTTTATTCTTTCATAATTTTTATGATTTTTTTTGGCATAGGCCGTGCAAATCATATCGCGGTCAATATTCATAATTTCGCCTAGTCCCAAATAAACACCGTATGCTTTTTTTATTAATCTTTTATTATGGTGCAAAAACAATTTGGTAAAGCATTGATATGTATAAGCAAGTTGCAAGGTAAAGTCATTATATTTAATTATTGGCTTTATATCAAATGAGGTTATGTTATTTTTTAGCGCAATGCTAGTAATAAAATGAATTCCATCTGCATATTCTTCTAACATTGAGATTTGGTTTATGCTTTTATCTTTTTTTCAATATTTAAATAGTTTTACTTCGTTTGCAAATTCTCCAAGTTCAACAAGTAAAGCAATAACCATTTTGATTTTAATATTCAAAAATTCTGTTTTTTCAATACTATTTGCAAATGCAAGGTCTAATTTCTTTTGGGCTTCAAAAACTTCTGTTAAATTCATGTTTTGAATTATAAACTTAAAAGTTAAAAATAATTCCATAATAAATTATTTTTTTTATTATAATTATTTGTATGCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCATATCTTTTGAGACTATTTAAACTAGCAGCAATGCTAGTTTTTTTGTATCTTTTATATCTAAAAAAAGAATTTGTGAGTCATAACAAGGTATTTTACTAAAAAATCAATTTATCAATGTTATACTTAAATAGTTAATTATAAAAATATGGGGAATTAAAAATATGGATAATAGGAAGCAAGAATTAAAACAGTTAAAAAAAATACCACTACAAGAGAATTGACCTAGAATTTTAATATTGCTAATATCTGCAATTGGTTTACTTGTTTTAATTTATATTACATTGGTCTTTAAAAACAAATATGAAGAATCTTTTGAACTTGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTAGGAATATTTTCAGGAGCATTATTAGTCTTGGCTTCTTTTATATTATTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAATAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAACTTAGTTAATGAAATTAATAGCGATTTTGCCAAACATTCATTTTTAGATCCAAATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGGTTAATTATCTTTGAAATTGTCATTCCTTTATTTATTTCTATTGCATTAATAGTATTAATTGCATGTCTTTTTAACAATGATGATCAACCAATCAGGGCAATTTATAGATTGATAATCATTTTAGTCAACGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTAAAAAATTATCAAAATTTCAATAATTTATATTTGCTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAGCAAAAGTACAATAAATTACAAAAATGTTAATTTATTTGAAATACTTTTTTCTAAAAGTTTTATAATTATACGTTTTAATAGGACTTATAAATGGACAATTTATGGTTTTATTAGTAATAATGAATTTTTGTAGCAATTTAGGTCTAAATTGTTAATTTTTTGTCTCCATATTAATTTAAATATATTAAAATTATCTTTGTGGAAACACAAGATTTATTTAACGCTATTAAATTTTATAAATTAATCTCAACCCTAGTAACAAGCTAGTTATTTTTTGGTTGAGATTTTTTAATTTATTTTAATTGACAAATATTAAATTAAAGAACATTTTTAATTATTAAATTGAAAGGAATTATAAAAATATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCCGCATCAGGACTTTCTGCTGCTTGTGGTCATACGGGTGGCGGTTCAGGAACTAGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCTTCAGGATTTTCTAACACAAATAAACAAGGTATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGAATCAAAGACAATAATAAACAATCAGAAGGATATTTACCTGTTGAAGTTAAAACATTGGCTAATGGATATAATACCAATACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCCATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAATGGATTTAATTTGGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGAGTAGTTCCTTTATCACGTTCATCAGAAATGCTTTCAGTTGCTAAAGCATTAGTAGGAAAATTCATTAAAGAATTAAACGGCTTGGGTGTAAAAATTTCTGACACAGACAATACTAAAGTTAAGGAATATCTAGACTATTACACAACAAATAAAAATGAAGCACAAGGTGTTGATGAAACTTGAGCACACGCTAAAACAGAAAAATTAGATGAAATTAAAGAACAAATAGTTAAAGCATTGCCAGAAATGTCAGATCAAATCTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACCAATGGAGATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGTGGATTTGACTACTCAACATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAAGATAGTTGACTTAATCCTAGAAGGTGTTAAAGAAGGCGCTGTTTGAATTGGTGGTGCAGGTGCTTATGGTTCAAACAACTTACTAAAATATCAATTAGCAATGAGCCTTGGATCAACTGCCGGATACTCACATACATTCTCAACTGCTAAATCATTAACAAACTTAAAAATAAAAGATACAACTACTTCATTAGATCAATCATCATTGGAAGTTTTAGGCCAAAAACCTGGTGAATCAGATAAATGAGTTATTCAAATTAAATCTGGTAAACATACAAATGGCGTATATGGCAAAGATAATTGAGCAAAAGCTTCAAAATATGATAAGAAAATAACTCAAGAAGCTGCTAATATAATTAAAGAAAAAGTTCAAGGCCAAGCAGGATATTTAGTAGAATTATCTTCAACATTTGAATATAAAAATAATAAGATTTCTCTAAAGAAAGGTAATAAGGAATTAGATGCTGTTTATTTAGGAAAAATTTATGAAGGAAATGAAAAAGAATATTTCTTCTTAAAGGAATCGCAAGTTAAGAAAGAAGAAGTTTCATCAAATAAAATTTTAAATGAAACAGATGCTGATTGAATTTCATCACCATTAGCATTTGATAAAACCGCTAAAAAAGCAGTATTTATTCAAGGACCTTCAATTCTTCCAATTCATGCTAACGAAAAAGAAGACAAGGCAACTAAATTATTCATCAACTGAATGTTCCAACACGATTTAGAAAACTTTGAAATTGTTGAAGTTTCTAAAAAAGGAGAAACTAAAACAACATTTGACGGTAAGCAAAAACCTATTGATATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGGTAAAGCAAGCAAATTAAATAAAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTACATTCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCGTTATCAAAAGCAAACGTTAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAATAGATTTTTATTAAATATTAATATTTAAAAAACATAAAAACAACCAAATTTGGTTGTTTTTATACGGAAATTAGAGAAAAATATAAAATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGCTCCGTTACTGTCCGTATCATGCAAGACAGTGGATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATGAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATGAAAAAGGAAATAAAACTATTAAAGAAGTTTCTCAATCATTATGGGATTATTATAATTATATTGAAGGCGAAGTTACTAGAATTTTTGATGGCGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAATGTTCCTACAACAATGAAATTAAGAATTCCAATGATTGATACATTAGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGAAGTGAAAAAACATATGATAGATTTGTTGGATATGTCTTCTTTGGCAAAAATTTTGAAAGACAATTTGATATTGAAATGTTAGCAGGTGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCAGAATTTATTGCTGATTTATCAAAAGAAAGCACTGAAGCAAAGGAAATAAGAAGTTACTTGTTGCCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCATATGAACTAAGTTCAAAATATTTGGAACACGGACAAGGCATGGTTGATTTCTCACTTTCAATACTTCATTATAATTATTGAAAGAAGCCTAAATTGGCAACTAAAAAGAATAATATCTATAGATATTTAGAACAAATTAATCAAAATAAAAAATAAAGGGAAATTCTATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAGGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACAACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGGGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTCCAAAACTATGCACTATATCCTCACATGTCAGTTTACGAAAATATTGCATTTCCTCTATACAATGACAAACATTGACGTGAAGAGACTCTAGAAAAAACTAAATTATCACAATTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTTGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAGAAGTATTTAAATGAATTAATTTCACAATATAACGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACTTCGCTAACTAAAAATGTTTTGCAATATATTAAAAAACTAGGATTGCAACGCCAAGCAGAAATTTCAGAAGCTAGAGAAAAATTTTTGGGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTAAAAAATATATCAGTATTAAAATCAGAATTTTCAGATACTGTTTCTAAAATTAAATCTAAAAGTGCAGAAGAGATTGCTAATGTTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAACACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACGTGAACAAGCAGAATCTCCTTTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACTAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAATTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTGGATCAAGAAACCCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGACGAAGCAATCGTAAAAGATGTATTAGATGTAGCACAAAGAGTTGAAATTACAAAGAACTTAGCAAAACGTCCTACTCAAATGTCTGGTGGTCAACAACAACGTGTTGCCATAGCTAGAGCAATCGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGAATACGTTCAATTCAACAAGAATTAGGAATTACAACCGTTTTTGTTACACACGACCAAGAAGAAGCTATGTCAATTAGCGACAAGATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCTCAAATTAAAGACGGATATATAATTTACAACAATAAAAAACTACTTAAAGCTCCTGAAGGATATCAAAAAGCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAGGAAGAAGAAGGAATATTTGAAGGAAGCATAAAATTAGTTGAACATTTAGGTAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTACGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAATGAAAACCTATTTTTGAAAAAAATATAGAGTTAAAAACACTGGACTTGCCTTAAGCACATTAAATAACAGGGTTAGTTTTTGAAAGCCCTTCTTATTAATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATACCAAGTTGGTGCGACGGTTTATGACAAGGGATTTGGATTAAAAAACTATATTAAGTTGTCAAAAGATCCAATATTCCACATTGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATCTCTATTTTGATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGACAAACAGTATTCTTTCTACCATATGTAACATCAGGAATTGCTGTTTCAATCGCCTTTGCATTCTTATTTAGAACATCAGGCGGATTTATTAACATGCTAGCGGGTAAAAACATCCAATGGCTAGATAACCCAAATGATGGTAATTGAAATGCATTTGTTGTTATTTTAATTAGAGGTGTATGAGGAAATTTGGCCTTTCAAATTCTAATTTTAACAACCGCTATGCTATCGGTTAACCCTGATTTATATAAATCAGCTTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAAGCAGCTGCAAACTCTGGTATGAGTATCATGTTATACATATTCTGATATGTTCAACGTGGAGTAAATGGCGTTGCTGGAGCAGCATCATTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAAGCAAGAGTTAATGTCGAAACCTTACTCTTCAACTAAGGCTAGTTCATTAATAGCATCTTGAGCATTTAAAATAATAATTTTAGTATTCTTCGCGTTAGTAATAATATTCCCATTCTACTTTATGATTGAAATGTCATTTGTAGAAAAACAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGGGCAATAGTAGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGGTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCGATTACATTAGGATATGCACTATCATTAAGAAACTGAAGAGGAAAGAATTCGTTCTTTATCTTCTTTATATCGCTAATGATATTGCCGGAAGTCGCCTTACTAAGTGGTCAATACCGGTTAGTTGTAGCATTGGGCTGAACTATTGGATGAGGTCAATTCTTTGCATTAGTTATGCCGTTTGCTGCTTCAATATTCTTTGGATATATGTATAAAAATGCATTTGAAGCAATCCCAAACAGTGTTAAAGAATCATCAATGCTAGATGGCGCAAATGGAATCAAATACTTCTTTAAAATAGCAATGCCAATGGTTTCAGCAACAACCTGAACAGTTTGCATTCTTACAGCATTTGCATCATGGAACTCATATACATGACCTGCATTAATATTTAAAACCTTAGGTTCACCTTGAAAACCTTTAAACCTATGAGTATTTACTACTGGTAAATCACAAGAATCAGAAATTCAAATTGTTTATACATCAATAAGAATGGCTGCAACTGTAATTGCAATTCTCCCAATGTTTATAATTTACTTCTTATTAAAGAAGAGAATTATGAATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCGAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTATTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTATTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAAAAAACTGTAATAAAAAATATCGCATTTGCGGTATTTTTTATTGATATTTTTTAGTTTGCAAATATAATATTTAAACAAAGTCAATAAAAAATTTGGAGAGATATATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGAAAAAAAATGAGCCAATATGTAACTTATTATTTAAAATCCAGGGGTATAAATGTTTATTTTTTACATAAGAATCATTTAGGAGTTCCTTTTTCATTCTTGGAATTTGCTTTTAAAAAATTAAATACCTTGGATTTGGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAAATTCAAACTATTTTTAAAAATAATCAAGAGATAAATTTTTTTGATTTTAATAATTTCAATCTCAATGAAAATGAATATCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGTTGCTTAATGATTATTTAAGCCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAATAAATATCAATATAATTTTGAATTAATTAAGAAAATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAAATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTGTTAACAAGAGGCATGCAATTTAATTATTTTGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCGTATAAAAAATTATTTAATTATGATTTTAAGCAAATTTTAAATGAGCATTTAGTTGCATTATATATTAAATTTCAAAACACATTCTTTAACTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAATTTATATAAACCAACTTTATCCCACTCCTATTTTAATAAAGGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCAACAACAAGACATTGTTTTAAATGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGCGCTGAGTATAATTATAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAATTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAATTGAAATTAAAATTCTTAACTTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGACAAATTAAGTATTCAAAAATCAATATCTCGTATTGACATTGATCAATTAACAGTTTTCGAAAACAAACAAGACGCATATATAAAATATCTTGCTAAGATAACATTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCTTGCAAGTTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAGTATTTTAACAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAAGCGAATTTTTTAATATTATTCTTGGAACAAAAGCTTAATTTTATTAATAAATTCATCGTAGTCAAAAATTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCTCTTAATTGACCACTTCTTCTAGTTGCAACATCATCGGCAGGTTTGTATATTGATCCTTCTTTGATTAAGTGTTTAAAGTTTGTGAACGCTATTTCTTCGGCAATATTTTTTGTTACGTTCTTTGCATCTTGGCTTAGATATTTATTTGTAGGAGAAATATAACCACTAAAGATATTAAAAGCGTCTATTGCTTTTGTACCATCAGGGAATCTTTGAACATTTATCTTTTTACCCTTACTATCAACTTTATAAAGCTCTAAATTATTCAATGGTTGTGTATACATTCAATTAATAAATAATTTAGTTGCTTTGTCTTCTCTTTTATTTGCATGAACTGGAATTACAGAAGGACCTTGAATAATGATTCCTCTATATTTTTCATTTTCTGAATATGAATAAGGGGGCGATATTATATCTACATCTTCTTGATTAATAAAATTATTAGCATTTAATACATTGACCTCTACATCTTTTTTGTCAATAAAGAAATATTCGTTATTGTCATTGTTAAAGAATTTTCCTAAATAAATTCCTTTAAATTTAGTTATTTTATTTCCAATTTTTAATTCAATTTTATCCTCAACTTTGTTGTATGAATATTCGGGTCCTGAAACAATAAATCCTGCCTTGCCTTGTAAATTTTTAGCTTCGTTTGTTGATAAAGAAGATATTTTTTTAATATATCTAGGATTTTGTTCGGCATCTTTTATATCTTTTTCACTAGCATCTGCTGTTCAAATTGTATTCGAATATTTATCATTCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTGGGAGTATCGTTTAATATTTCAATTGTTGTGTTTGGATTTAACACTCTGTTTGCCTGCTTGATTGTATAATTGTTATTTTTTCCATTTTCACTAACAAAAGTGTAATGATATGCAGCTGTTGAAGTAATAACCATTGCTAATTTATAGGTGGTAAAAATGCCCGTTCCTCAAACACCTTGTTTAGCTACTCAAACGGCACCTTCTTGAATGCCCTTTAAAATAATAGAAGAAACTTTTTTAAATAATTTCGCTTGCGGGCTATCAGGTTTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGTTGGACAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCCGAAGGTATTTCATCTATTCCCAAAACGTTTAATGTAGGGTCCGCGTTGTATAATCTTTTTGCTTGAATTGCAAAATTAATTAAATCGCTGTAGTTGTCAAATAATTTATCTGACATTTCTGGCAATTCTTTTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATACCATCAACGCCTTTTGCTTCTTGATCGTTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTACTATTTTGACTATCTAATTTAACTCCTAGAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTACTGTAGTCATTTCTCCTGAACGAGATAGAGGTATTGCCCACTTTTTATTATATTTGTTATTTGCAATTAAAGAGTTTGCGACATTAAATACTGGTTCAATGTTATATTTTTTAAAATCTTTGTCATCAATTGCTAAATTCATTTTGTGATTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATGTTGTGAAAATCTTTCCTATTTTTTACTTTTAAATCAAGTGCAATTGAAGATGTATTATATCCTGAAGCTAGTCAGGAAATTTTTAAAGGCAAGTACCCTTCAGATTGCTTGTTATTGTCTTTGACTCATTTGTTGTATGTATCAACAATCCCTTGAATAGCAAGCCCTTGCCAATCATTTTTTAAAAATCCCATTGATAATACTAATTGGCCATCATCTTCTTGGTCAAATCCGTATTTCGAATTTTCTGTTGTTGAACATGAGGATAAAATCAATGTAGTCGTAGGAAGTAAACCTATAGTCATTAAAGTGAATAATTTGCCAAGTCTTTTCATATAATCTGTTTTCCTTTAAAAATGTATAAGAAATGAACTACAAATTCATATAACCGTGTTGTTCTCTACTGGTTTTAAAACCATGTAGTAATATAAAATATATTTTAAATATAAACCCAAATTTCTTATTTTAACTAATGAAATAAATTATATATTTTTATTGCATTAATTTTTAGAAAACAAAATTCTTTATGCACTTTTAGAAACAAAAAAATGTCTTATTAAATTAAAAAATTCGCAACTAAGCGAATTTTTAATATTATTCTTGGAACAATAGCTTAATTTTATTAATAAAATCTTTGTAGTCAAAAACTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCCCTTAATTGGTCGCCTTTTCTAGTTGCAATATCATCAGCAGGTTTGTATATCGATCCTTCATTGATTAAATGTTTAAAGTTTGTGAATGCTATTTCATCAGCAATATTTTTTGTTGCACTCTTTGCATCTTGACTTAGATATTTATTTGTAGGAGAAATATATCCACCAAAGGCGTTGAAAGCATCTATTGCTTTTGTACCATCAGGGAATTTTTGAATGTTTGTTTTTTTGCCATTGCTATCAACTTTATAAAGTTCTAAATTATTCAATGGTTGTGTATACATTCAATTAATAAATAATTTAGTTGCTTTATCTTCTCTTTTATTTGCATGAACTGGAATTACAGATGGTCCTTGAAGAATAATTCCCTTATATTTATCATTATTTGAATATGAATAAGGTGCTGCTATTCAATCTGCATCTTCTTGGTTAATAAAATTATTGGCCTTTAATACTTTAACATCCACGTCTTCTTGATTAATAAAGAAATATTCATTTTCATTATTATTAAAGAATTTGCCTAAATAAATTCCTTGTAATTTAGTTATTTTATTTCCTATTTTTAATTCAATTTTATCTTCAGCTTTATTGTATGAATATTCAGAGCCAGAAACTATATAGCCTGCCTTGCCTTGTAATTTTTTAGCTTTATTAATTGACAAAGGAAATACTCTTTTAATGTATGTAGGATTTTGTTTGGCATCTTTTATATCTTTTTCACTAGCATCTGACGCTCAAATTGTGTTTGGATATTTTACACCCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTAGGAGTATTATTTAATATTTCAATTGTTGTTGTTGGATCTAATACCCTGTTGGCTTGTTTAATTATATAAGTACTATTTTTTTCATTATTGCCAACGAATGTGTGACGATATCCAGCAGTAGAACCAATATTTATAGCCATTTTATAACCAGTAAAAATACTTGATCCATAAACGCCTGGTTGAGCTACTCAAACAGCATTTTCTGAGATACCTTTTAAAATAATAGAAGAAACTTTTTTAAATAATTTTGCTTGTGGACTATTAGGATTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGCAGGGCAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCTGAAGGTATTTCATCTATTCCTAAAACATTTAATGTAGGGTCAACATTATATAATCTTTTTGCTTGAATTGCAAAGTTAATTAAATCGCTATAGTTGTCAAATAATTTATCTGACATTATTGGTAACTCTTTTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATACTATCAACACCTTCTGCTTCTTGTTTATTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTACTATTTTGACTATCTAATTTAACTCCTAGAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTATTGTTGTAATTTCTCCTGAACGAGATAGAGGTATTGCTCATTTTTTGTTATCTTTATTATTTGCAATTAAAGAGTTTGCATTATTAAATACAGGTTCGATGTTATATTTCTTAAAATCTTTGTCATCAACCGCTAAATTCATTTTATGGTTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATATTGTGAAAATCTTTTCTGTTTTTTGTTTCTAGGTCCAATGCAACAGAAGAAGTGTTGTAACCTGCTGCTAGTCAAGAAATTTTTAAAGGTAAATATCCTGCTTGTTTTCTTTCTTCTTTGAATTTTGGATCATTTATTCAATTATTATATGTATTAACAATTCCTTGAATGGCAAATCCTTGCCAGTTACTTTTTGAAAATCCTGTTGCTAATACTAATTGTCCATCATCATCTTGGTCAAATCCATATTTTGAATTTTCTGTTGTTGAGCACGAGGCTGAAATTAATGCAGTTGTGGGAAATAATCCTGCTGCTACTGCTGTAAATAATTTGGTAATTTTTTTCATATAATTTATTTTTCTCCTAAAAAAAGTGTATAAAAAAACGAACTACAAATACAAACATTTTGTACAGTTATGTTCTTACTAGTTTTAAAAAACGCTAGTAATATAAATATATATATTTTTTATTTTTTGTTTCAAAAATTTTTATTTTTTTTCAGCCAAAAATATAAATCTAAATTTTAAATTTTTGCTTGTCAAAATAAATTTATATATTGTTATTACATTATTTTTAAAAAATAGATTTTTTTATAAAAATTAGTGTAAAATAAGCATGCAATGACTCAGCATAAAAATATTTTTTAAATAATTTTTGCTACATTGGATTGAGAGTAAGAAAGCTGTAATCCGAAATCATTTAAGCAAAAATCTTAGGAAAGAATTTTTAAAGTCCTTGCATATGAAAGGAACTAAAAAATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGGAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCTCCTGGACAACACGGAGCAAAAAGAAGCAAACCATCAGACTATCAACTACACATGTACGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTACTACAAACTGTTGAATCAAGATTAGACAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCATGGACACTTTACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAATTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGATGCTATTGAAGTAATGACAGTTGCTCCATGATTAACAAAAGAAAACTTTAAAGTAACATTTACAAGATTGCCAGAAAGAAACGAATTTGCTAAAGATATTAACGAATCATTAATCGTTGAATACTACAACCGTTAATAAACAAAAAAACGAAGTTTGAACAACCTCGTTTTTTATTATTTATTTTTTAAATAATTAACTTTTTTAAAAAATATAAATTGTTTTTCTTTTGTTTTTGTAATTAAAACAATAAATAAGATAAATGCGGGTATATAAACTAAATTAATTAGATAAATATTATTGCATAAAGGATTAAGCGTCATTGTTATATTCGAAAGTGAAAACAGCGTATTTAAAATTTCAGGGAAAACTATAGAACTTAAAACAATAATCAAATAGTTTGTGAGTCTATCATGACTAATTTGATAAAAAAGAATACCCAAAATAAGTGATATTAGATATATTAATATAATTACAAAAGTAAACAATATTACTTTTTTGAAGTTATTGGTGATTTTGATTAATAAAAAACTTAATAAAAATCAATAGGTTAATCTAAAAATATAAACATAAAAATATTCAATAATTTTTGTATTTTCTAAATATACAGGAGTTTTTGAATGTCAATGTCCAGAGACGATTTCATAAAAATTTTGTTTAATAAACAAAATCATTACGTTAATTAAAAATATCACAATTAACGATATTGCAGTTGTCAATATTATTATTAAATAGTTTCTTCTTTTTTCTTTGTGATCAAGTTCTATGCTTCGTTTTGATAAATATCGTTTAACAAAATAGAAGTTTAAAAATAGTCCTATTGATAATAATGTTAAAAGAAGCTGGGTAGTATAAGAAGGCGCGATTAAAAATATTTGATTTATTAAAGGAAATAGATTAAATTGGTCGATTTGACCATCTATTTTTAACATTATTGCTGATATTAAAAGATAAATTACAAATCCTGCTAGCAATATTCAATATGGCAGAAATAGTAGTAATTGTTTTTTATTTTTTTTCATTAACAAATTTCTCCATTTCTCTATGCATGGTAAATAAATGAATATATAGTTTTAGCCCATATGCGATAATTGATTTTATTTTGGCTGTTGTCAATATCTAATTATTTTTTAGTTTCTTAGATATAATATCATTTGCTTTTTCAATTCATAAACTCATTTGGTTATGAATGTAATTGTAAAATCAACTAGATTTGTTTAAATATTCTTCATTAAATTTTTTTAATTCGAAACTAAAAGTATTTGCTGCTTCTAATAGTAATGCTTCGTATTTTGTATTATCCAATGTTTTAAATAGTTGTTCAAGTTCTTTTTTTGCATCTTCTGGGTTGCAATATATATAAAAAGGTTTGATAATATTATTTTGGATTTTTGTCATAATAATAAAGCAATAATTAAGGTCTTCATCATTGACAAAATTTAATAGTTTTTTTATTAGTTTTTGAATAATATAACTTGCTTGGTAATTATCTTCATCATTTGAGTTGCTGAATTTAAATTGGTACTCTGTTTCTTCGTCAAATAATTGATTATCAGCAACAAATGCTTGGTTTATATCGCAATATAAACCTTGAAAATTGTCAATGTCATATTGACTTAAATTATTTGATATTGAAGTTAGAAGTACTTCGTTATCGTGTTTAATTTTTGAAGCCTTTGTATAAATTGTAAATTCGATTGTTCTAACAAGAGGAATTAATTTATATTCATCAACATCATTCAAAGGCAATAGTAATCTAGAACAAATCATATATTATCTCCAATCTAATAAAAAAACCACAAGACATAATACTTCTCGATATGGCTGCTATATTTCTATCCTGACCAAGTTACAAGGTTATCATTCTCATGGCATAATAATTATACTAAAAAAATAACTTTAATTTTATAATTGAAAAAATAATAAATTAATTTAATTCTAAACTTTGCTTTAGTTTATTTATTATCATTTTTTATATTTTACGAATTATGAGATTAATTTCTTTTTTTGCAAATAAAAACCTCCCTCATAATATTTTTTATAAAGGAAGGCTTCATAAGTTTATGTAAATTTTATAGTTTCTTATTTTTAATGGTATTAAATTTCTTAAAAAATATGAAATTTTTTTCTTTGATTTTTGCGATTAAAACAATAAATAAGACAAATGCTGGTATATAAACTAAATTTATTAGATAAATATTATTGTATAAAGAACCAAGTGTCATTGTTATATTTGAAAGTGAATATATAGAGTTTAAAATTTCGGGAAAAACAATGGAACTCAAAATAATAATTAGGTAGTTTAAAAATCTATCATGGCTAATTTGATAAAACAATGTGCCTAAAATAAGTGATATTAAATATACTAATACAATTACAAAAGTAAATAATATTGCTTTTTTAAAGTTATTGGTGATTTTTATTAATAAAAAACTTAATAAAAATCAATAAGCTAATCTAAAAATATAAACATAAAAATATTCAATAATTTTTGTATTTTCTAAATATACAGGAATTTTTGAATGATCCCTTCCATCCCTTATTCTATAATAACTTTGATTAATAAATAAAATCATTATGTTAATTAAAAAAACACTAATTAATGCAATTATAGTTGTTAATATTATTATTAAATAGGTTTTTCTTTTTTCTTTGAAATCTAGTTCGATATTGTGCTTTGTTCAATATCTCTTAGCAAAATAAAAGTTTAAAAAGAGTCCTATTGATAACAACATTAAAAGAAGTTGCGTAGTATATGACGGGGCAATCAAAAATATTTGATTAATTAAATTAAATAAATTAGTATTGTCAATCTCACCATCTACTTTTAATATTATTGATGATATTAAAAGATATATTGTAAAACCACCTAGTAATATTAAATATGGTAAAAATATTAATAATTGTTTTTTAGTTTTCATTAATAAGTTTTCTCATTTCCTCGCCAGTATGCATATTATTTTTATAATTAAACAATTTTTTAGTTACTAAATTTTGGTTAGCCTTTGGCCTCATAGTAAAAATTATTTTTAATGGTGAGCCAGAGGCTGCTAATTTTCAATAATTTACTAGTACTATAGAGTCATTATTTCCTCATAATCAATTTAGTAGAACCATCTGAGTTTCTTCATCATATCCATATATTATAAATGCGTGCATAGTTTTGGCGGTACTTACAATTACGGGGTTATTGTTTTTTATATTTTCTGTTGCCTTATAATAATAAGCACCTTTTTTGTAATCATACTCATACTTATTTTTTGCTTCCTTATTATTTAAAAACTTATCTATTGGATATGTATAAGAATTATAAGCATAAAAATCTAGTCACCTTTTATTAAGCCTTCATAATTTAGCAACTAATGAATAATCAGGATGATCATATTGATAATATCTAAAAATAGGCGATGAATTTTCTATAGAATCGTCATAATTATCATATTTAAAAAAATCATTAAATTCTTCATTTGTAAAAATACCACTTTGATTAAAAAGTTCATTATAAAGTAAAATATTTGATATTGCAACATATTCACATAATCCAGAAGTATCATTGTATTTATCACTCAAAAAAGTTTGATCTACATATCCTACTTTTTGCATTGTGTTTCTAGTTGCTCACCACCAACTATTTTTAACCATATTGCTTATATTTGGCAATGTATCTAGTTTGTCTTCCAAAACTGGAGAAAAATAATCTTTCTTATATGCCATTCTTAATTCATTAAAGTTTTTATTTTCTTTTTCTAAATAATTAAAATTATATTTTATGTTTTCATTATAAGAATGAAAACCTTTTTTGATTTCAGTAATTGAAAAATCGTTTTTATCAACGATAGCATAATAGTTAGAACATTGAATGTATAAATATTCATTATTTAGATTTTTTATATATTTAAATTGTTGATTTTTTCCAAAAAATCTTTGAATAGTATTGGTTACTTTTTCTTTTAATTCTTTATAGTTTTCATTGTTGTTAATATTGCTAGACAATAAGCAACAAGGCATTGCTGCTACTATGGTTAATGCAGGAATTTTTATTTTCATCATTTGGCCTCCTAAAAAAATTATAAAATTTTTTGCTTAAATATTAAATTAATTATGAACACATTTTATAAAATCAATTGTCTTTTTTAAAAATGTTTAATTAATTTTTTGTTGTCCTAGTCATGCTGAAAGGACACTATTTACTTTTTTTAAAATTTACTAAGTGCAATAAAAAATGCCATTCAATAAATGCATTTTTAGCAAAATATTGAATGGCATTTATTACAATAAAATTTTATTTTTTGTTGCCCTTGTAGTTGCTATTGTTTGGGTTCAATTGATTAGAATGATTGTTTAAATTTGATCTGTATGCTGAATTATTAGAATTATTTTGATTTGAATAATTATTCATTTGTTGTCTTGTATGTGTGTTTCCACTTACTTTATTATTTTTGTCATTCATAATTTACTCCTAATTTTTATATTCATTCGGCAATCCATAATGATCACAAAGCATATTATATGCTGCCATTTTTTTAGCAAGTTTTTTTGATGTGCCATATCCTTCTGTGCATTTAGTCCATAAGCCCATTCATTGAAGATTTCTAAAATTAACATTACATCTGCAATGTCATCATTGTTTACCGTTATTATCAATTATAGGTTTTTCTGATATTTGATATATTGGCATTGAGCATTCACCACGCTCTGCTAATTCTTTTAATGTATTGATAGCATTTTCTAATTTAAATTTATCCGGTCTTTTTTCTCTGCAATCAACATCTCTAAAAAAATGTTCAATATCTAACATCTTTATAATGATGTTTTTTAAACGCTTAATATCTCAACCACTGTCAATTGCTATTGCACCAATTATAGCTTCAAATAAATCAGCTTTCACACTATCTTGATTAGCAACATTATTTCTTTTATCATTATCTCCTAAAACTAAATACTTAGCAAAACCTAATTTATTAATTTTTTTAGCTAGTGTTTTATTGCTTACGATTTGATTTTTTAAATCAGTAAAATCGGATTCATTTTTATTGGCTATAAGGCAATATTCTTCAAGATCTTCATATTTGTTGTAATATTGCGAAGCCGATTTTAAAAAGCCAAATTCTTCTGCCATTAGTTGAACTACGCAAAAGCTCAAAACTGAATCTCCTATAAATTCAAGAACTTCATTATTTTCAACTCCATATTGATTTGAATAAGAACTTCTAGTAAATGCTTGATAAAGTAAAAATTTATTTTTGAAGAAATATCCTATTGTATATTCAATTTGATAAATATATTCTTGCCATTCTTCTATTCCCATATTTGAATTTTTCCTTTCTAAAATAATTATTGTTCAATAGTTAAAATATGCTTTTGTTGTATATTTTAAATTCTATTTAATTAAGGTAAAATAATTTTTATAATATAGTCCATCTAAACTTCTTTATTTTTAAGTGGCAAAAAAGTTTAAATAATACTTATTATTCAACTACTTTAACATAACTAAATTATTGAACAATTTAATTATGTTTTATATTAAACTATGACTTTCTTTTATTTAAAAATAAATTTAAGCGTATATATTAATTTTTGCAAAATAATATTGCAATGTGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTGCCAAACTCAAAGCCAATGCCTGCAATATTAAATGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGATTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAGTAAAGAAGAATTATTTTTAATTGATAGATGAGAATTATTTCCTCAATATCAAAGATTTCAAGTCAATGTTTTATTAACCGAAACAAATGAAATTTTAGAAAATGTTTATGTATATACAAAATTAGAAGTTGGAAAATATTATTTAGCAACAGATGATATGGGATTTTCTAGAAATCCTAATGCCAATGAAAATAATTTAAATGCTTTTATTGAAATGGAAAAGGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGATATAAACGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCTTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCACGGATCAATTTTTGCAATTAAAGAAGATGGTAAAATGTATGCAGCCTTAACTTCGTTTAGCCAAAAATCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCTGAAATAACGTTAAAATCGTTATACGATAATACAAATATAGATTTTTTAATTAATAAAAAGCCTGTTTATTATTTAAGTTCAAGAGAAGACAAAACAATCAATGAAACTCAAGTTGGTTGATACGAAAATAAAGCCTTTGAATTAGTTGAAAAAGATTTTGACATTGATCCATTTATTCGCTTTAATAAAATGTTAAAAGCGTTTTTTGATACAAAACAAAAAAATGATAAATAAAGAAATAATTATAAAAAATGTGCCACATAAACCCGGAGTTTATTTATGAAAAAATAAAAATGGCAATATTATTTATGTTGGCAAAGCAAAAGATTTGAAAAATAGAATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCCGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTACAAGCCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGATATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCTGACAATAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAAGATGGAATTATAATTGAAAAACAAAGTCCCGAATTTGCAAAGCAGAGATTTGAACAAGCCAAATCAATCATGAAATTTGGTCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAAATGAACCTGTTTGAGCAGGCGAGATTTTATGACCAAATTTTAGATTTGTTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAATATCAAAACTATATGTTGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTATGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTTATTGATGAATTTTATGTTAATAATCAATTGCCCGATCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAATGACAAAGTAAGCGCGCCATTGAATAAATTAAATAAAAACTTGATCGATATTGCTATTGAGAATGCATCAAATAATATTGAACCAAAAATAAAAAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCGATAAATTATGTTCATATTTGAATGTTTATGCAGAAAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGAGCAGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCAGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTTGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGCTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAATAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAAGTTGATAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATTAATTATTTTGGCAGTTATAAAAAAATTCAACAAGCTTCTTTTGATGAAATATCAAAGGTGGCAAACAAAAAAGTTGCTAAGCTAATTTGTGAAGACAAAGGCGAATCGTTTTATTAAATTATTTTTCAAAAATATTAAAAAAATTTTTAAATGGTGTAAAATAATAAAGCAACAAAATAATGGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCATTTTTTTTATTCATTTTTTTAAAAAAATAAAAAATATGTATATAATTATTAACGCATACAATTAAATACGCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCATTGCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCATTTGAGTAAAGTTCACCAAAATTGACATAGGCTAAGGCTTGTGTCAATTTTTATTTATAAAATCTCATGAATACTGTTATTGCTATATTTCATAAAATCTTAGGTCTAACAGTTATTATGGTTAATAAACATCGGCTCTTTTTGTTATTATTTTCTTTATTTTTTATTAAATAATAAGGTGCAATAAAAAAATTCAAATTAAAAATTAAAGTTTGAAATATGAATAAAAAAAGTATGCAAAGACGTACTAAAACCATTATGATATTATTTTTATTTTATATTGAAATAAGCATTTAATTATGAGTTTAAAGTTTGCTCTATTTTAAGGCTATACATCTTTTTTGTTTTTAAAACATATTGATGTTTAATTTATTATAATTAACTTAATATTTAGCAAATACATTAATTTACTAAATATTAACCTTAAAAATATAAAGGAAAATTATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAAAAATAATATTAACGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCCTTAACTGACAAGAACTTTAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTGCAATTAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATTGCAACGCAAGGAGCTCCATTAGGTTGATATCCATGAGCAAGTCATATTAATTATCTTGAAGGAATGTGAAAGTTTTTAGGTGCAAATCTTTCAGAAAGCATTTTATTAGCTGGCACAAAAGTTGAACCTTTAAATAAAATGCAACCTCAAGAAATAATTGAAACTATTAGCAAAGAAATTATTAAAAAAGCAAGCATATTTTAATTATTTTTTGTTATAATGTAAATGCAATTTTTATTGCCACATTAAAGTGGAAGAATTTGAAACAAATTCGTTTGAACCACTATAATCGAAAGGATTTTATTTATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCCGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATATAAATTACCTGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGACTAAAAGGAAGTGCTAACTAATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTCCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCCGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTACTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCTGATCCTAAAATGATGTTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAGGCCGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGCGGAATTGTTCATGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTTTAGCAGAAAATGCTGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAGGGTACATATGTTTTAAATATTACCGTTTCAACTTCTATGGGTCAATCTGTTAAAGTAAGAATTGATTAATATGTTGAAAAAAAGCAGTCTTAACACTGCTTTTTTATTTGTTTTTAATAAAAAAACAAAATTGTTAAAATAAAAAATACCTCAAAATTAGAGGTATTTTTTAAATTTTGTTAATTTTTTAGATTATAAAATGAGTTTTTGCCTTCAAATTTAGCTTCATTGCCTAATTCTGCTTCTATTTTTAACAAGCGATTGTATTTAGCAATTCTATCTGTTCTTGAAAGAGAGCCAGTTTTGATTTCCCCAGTATTTAATGCTACTGCCAAATCTGCAATAAATGTATCTTCAGTTTCACCTGAACGGTGAGAAATTATTGCTACAAAGCCAGCTTTTTGTGACATTGTTATTGCTTTAAATGTTTCAGTTAATGAACCTATTTGATTTATCTTTATTAAAGATGCATTGATTGCATGTTTGTCAATAGCCATTTGAATGTATTTTGGATTTGTTACAATTAAATCATCTCCAACTAGTTGAACTTTGCTGCCAAATCTCTTATTCATTTTTATAAAGCCATCTCAATCGTTTTCATCATGTGAATCTTCAATAGAAATTATTGGGTATTTTTCAATTAAATGGCCATAATATTCAACAAATTCATCGCTAGTAAATGAATATTTAACATTTTGATATTTTTCAAATCCTGGGCGTTTTTCTTCAATTGCTTTCTTGAATTTTTTAAATACATATAACTTTGTATCTGAATGATAAAGTTCTGAACTTGCAGCATCCAAACAAATTGCTACTGCTTTTTCTCCTGAAGTGGCTGGATTATATCCTGCGACTTTAATTGCATTTACCAAAAAGTCCAATGCTTCTTCATGAGTGTGTAGGTTTGGTGCAAATCCACCTTCATCTCCAACTTGTGTTCCATGACCTGCTTGTTTCAATAATTTTGCTAATGTATGAAATACGAAGTTTGCCATTTGCAATGATTCTTTAAATGTTTTTGCCCCAATAGGCATTATCATAAATTCTTGGAAGTCAATAGTATTTGAAGCATGTTCTCCACCATTAATAACGTTTAACATTGGCACTGGCATTTTGTATGCTACTTGGTTGTTTAATTTTGCCAAGTATTTATATAATGGAAGATTTGCTTCAATGGCACCAGCTTTTGCAATTGCTAATGAAACAGCTAATATTGCATTTGCCCCTAATTTTGATTTTGTTTCAGTTCCATCAAGATTAATCATTAAATTATCTAATTCTTCTTGCTTAGAAACTTCCTTGCCAATAATAGCTTTAGCAATAATATTATTAACATTATCGCATGCTAATTGCACACCTTTACCGCCAAATCAATTATTTTCGTATTTTGTTCCTTTGTCGCGTAATTCAAGTGCTTCTTTTGATCCTGTTGAAGCTCCTGATGGTACTAATGCTTCAGAATATGCAAGTTCAGTATTTAATTCAACTTTAACTGTTGGATTTCCTCTACTATCTAAAACTTCATAGGCATTAATCTTAATAATTTTTGACATAATTCTCCTATATGATTATTTAAAATAAAAGCTAAAAATAAATGAACATCATTTATGTTAGTTTACATAACAATTATATTATTAATTTTTAAAGGTAAAAAAATAATTGTTATTTTGTGTGTTCAATCTAAAAGAAACATAATTTTAAAAATATTTTTATGTAATGAAAAATTGCTATTTTAAATTAGTGTAAAATTTTATTTATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAAATTGAAAAAATTGCAAAAGAAGACCCACTTAAAGCAATTTATATTTTAGATGAAGACAAAAAACAATTCTCTCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAGTTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAATAGTTTAAAAAACAAAAAAATGGGTTATGCATTTATGCTAAGCTACAACGAACTTAAAAAACGAGATGATATAGCGAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGCAATATTGATTTTGAATACGATATTCAAAATAAAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAGAATGCTGAAATTCAAAAGATGCAAGATGAAATTATTGCAACTTTTGATAAAGATATTTCAAAAAACAAGGTTGCAAGGCAAGTATTTTCAGCGTATTTATTTCAAAATTGAGTTGCAATTTTATTGCACGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACGCCAGATTCACTTTCCAAAGAAGAAAAAGAGTTATATAACATTTTTAAATAAATTATTATGTATAATTTATAACATTAAACTAAAGGAGCATTTATGTCAAGAACATTAAATAAAAAAGAATCAGAATTAACTATTGAATATACAATTCAAGGTGAACAATGAGAAAATGCTAAAGAAAAAGCAAGAAAAGCATTAAGAGCCAATGTTCAAATCGATGGTTTTAGAAAAGGCCACGTTCCCGCTTTTGAAGCTGATAAAAGAATAAATCCAATTGAAGTTTATCAAAAAGCATTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGACGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTACAAAAAATTGGGTGTTAAATTAGGATCATGTGAATTAACTAAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGACGGCGGAGAAGCTGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATTGGCCTAAAAGCAGGAGAAGAAAAAGATTTAGATTTAGTATTTCCAGAAGATTATCATGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTTGTTAAAACTCCAAATTATCCAACAATCGATGATAATTTTGTAAAGGAAATTAATTTGCCAAATGTTAAAAACGTTGCTGAATTTGAAGAATATGTAAAATTTATTGCTTTAAAGAACAAATTGGCAAATGTTAAAAATGAATTTATTAATGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAGGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTACTGAAAAAGATTATTACGAATTTTCAAAGAGCACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAACAAATGTTTGTATTAGGCGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAACATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGCGAAATTTTACTAAAAGAAAATGACACAAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGATGAAGCTAAACGTAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAATATAATTTAAAAAGTGCCATAAAAATTGGCATTTTTTATTTATTATTTTTAACAATCATTAAAAATAAAATAATATTGTAAAATATTAACTATGAAAATATTTGAATTTAAATCAACTGAAATGAGAATTGCAGAGGCAACCCTTAAGAGGATAAATGCTTTAGAACCAGAAATATCAAAATTAAGCAACGAGGAATTGCAACAAAAAACTATTGAGTTTAAGTCGCGTTTAAAAGCAGGAGAAACACCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGTAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGATTTAGGTTCTGTAGCAGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCAACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTAAGTGTTGGGATAAACAAATCTCAAATGTCAAGCGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTGGGTTTTGACTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGTGGGCTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGCGATAATGACGAAAGCAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGCCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTATTTAACAGATAAAGGAATTGAAAAAGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTGCAACAAGCAATTCAAGCAAAAGAACGCGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATTACTTATCAAAATTTTTTCCGTCTATTTAAAAAAATAAGTGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGGGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCACAGCAAATGAAAGGCAGTTACTGCAGAGGTTAAACGTGTTTATAAAAAGAAACAACCAATATTAATAGGAACTGCTCAAGTTGAAGATTCTGAAATATTGCATGAATATTTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCAGAAGCAGAAATTATTTCTAAAGCAGGACAAGTAGGAGCAGTTACAATTGCCACAAACATGGCCGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGCGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCAAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGCCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCATTAGATGACCAATTAATTTTGCGTTTTTCAGTTCAAGATCACTGAAAAGAAATTTTCAAAGAATATGGCGAAGATCCAATTCCTGGTGAAGCAATTAGAAAGGCCTTTTTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTTGATGACGTAATTAGACAACAACGTGATTTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTCTTTATAAAAAACAATAGTTTGGATTTAATAGAATTTGTAAAGCATCTAAATAACAATTTTATGATTTTAACAAACAAAGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGTGAAGAATTAATTCAATACTTAATTGAAACCTGAAATAAATGTTATGACCAACTAAGACAAAATATCATTGATAAATATGGGATATCTTCATTAATAAATTCTGAAAGAAATATAATATTAAGCGTTTTTGATGCTGCGTGACAAGATCATATTAATATAATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCATATCAAGTTTACACCCAATTGGGTTCAAAGAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGCACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAACGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAATCAGCAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAACGTGATCATTTGATTAAAGAGGGTTTAGGCGAACAAGAAGTGGATGATAAACTAAAGGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAACGAGTGTTTAAAACTCGTTATTTTTTGTTATATATTCGCAATAAAATTCAACAGCCTTTTTCAATATTTCACAATCACTTTCGTATGTTATGCGATATAACAATGCATTTGTTGTATCTTGCCATTCAACTACCTTAATTTCACTATCTTGCTTAATTAAATCAAAAGATGTTGGCCTAGCAATAAAATAAACAACTTCTTTAGATATTAATTTTCCAATAATATATTTATTGACTTCTCTAAAACCCTTGAGCAATTTTATATCTATGTTAGTTTCTTCTTTTACTTCTCTAATCGCGATTTCCTCTTCTGTTTCGTTTTTTTCAACATGGCCTTTTGGGAACCCTCAATGGCCTGCTGTTTGTTCAACTAATAAAACAAAAAGATTATCATTTTCTTCTTTGAAAACAATAGCGCCACACGACTTCTCTTTTTTCATTAGCGAAATTTCCCTTACCTTTCTTAACTACAATTTATTTATATAAATTATATAACAATAAAGAAAATGAGCGCCTTGCCAAAAACTTAATTATTCATCTTTTTAAAAATAAAACTACATAATATTAATGATGAAGCAATTGCAACTAAAAATATCGTGCTTGAAATTCACATTTCAACTTTAATTACTTTGCCTTGTCAAAGTATACTTTGTAATAAATTATTATTTTTAGTCATTACTGCTTGAATGTATGTTTTTCTAATTGAAGGTAAAATTAAACCATTAACAATAAAACAAATTCCACATATCAAAATAAAAATGCTAATTATTAATAGAGTTTTTAATATCTTTTTGTTTGCAATTTCTGATTTAAATCTATATACTAAAAAGAAATTCAAAATATAATAAACAACGTAAAAACCGCAAATTATTTGTCCAAAGCAAGATATTGCAAGACGGCCATTATTTGAAAGCGATTGTATTGAAAATATCCCGGTAATCAAAAAAGAAATCAAAATCGCTAAAAAGCCAAACGCTGTTGTTATTAATAATCTTTTTATATTATTTTTTATAATATTGTTAATATCCATTATGCCTCTTTTGAAATATTATAAATGATAATAATAACCTTATTTTTGAATTTGGTAAAATTAAATTATTAAAAGGAGAAAATGTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGTCCCCACGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTACATAACCAAAGCGATATATTAAAACCATGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCGGCCTTTAACGCTTTATTGAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCTCAAAAAATACCGGAAACTATTTTGAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATTGCTAAATTAGGAAATGGTAGTCTTAGAGATTCATTGAGTATTGCAGACCATGTTGCAATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTACATGTCAATGCATGATATTAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGAATAATTTTTAATAAGACAAGAGATCAAAGTCTTGTTTCAACTGATTTTAAAATTTTATTGCAAATTAAAATTGATGATGTTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCTATAAGCGATAGTCCAGAAGATTATTTTGAAATTTTGTTATTAAAGTTATGCAACTTTTTAAGCCCAAAAGAAGAATATAAAAATTCTGCAGTTATGCCAAAAATTAGTAATGAGGAATTTAATAATTTTAATAGTCAAGAAAAGCAGGCCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCACAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAAACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGCAATAATCAAATTAGCATAGATTCAAATGTTGATAACCAAGAAACAACCGATTTACAAATATCGGAAAATTGAAAAGATTTAGTAAATGAGGATTTAATTTCAAAAGAAAATTCAGAAAATAAACATGTTCCTTTAGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAACAATATGTTAAATGATGGTCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAATATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACGTGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGCTTATGCCATTACAAAAGATGAGATATTAGAAGCAAAGAAATATTGAAAGGCAAATATAAACGAAATAAGAGCCAAAAAAGTTAAACCTTTGGAAGATTTAAATTTAAAATACAATAAAACAGTTCAAAAAGATGTTGCTTGAGCGCAAGAAATTTTTGGAGATAAGTTCAAGTTGAACAAATAAAAATTAAATAAGGAGAGAGTAATATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGACGAACAAGAAATTCAAAAGAAAGAATTTACTATTGAAAAGCAAGGTATAACCGTTGTTATGAATGGTTTGAGAAAATTAGTGTCTATAAAATTTAATCCTGCATTAATCGATCCAGATGATCCTGAATTATTAGAAGATTTATTAATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAATGAACAATATTGAGATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAAGTGAAGATATAGATCAAATTTGCAATCAAATAAAAGCATTGAGAAATTCAATAAAGAAGTGTAATATTTGCAATAGATATAACGAAAATACAATTTGCAATATTTGTTTGGACACTAATCGAACAAAACAACTTATGTTAGTTGGCAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAGCTTAAAGAATGAAGATTTTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGTCAATTTAATATGCATTATATTAAAAAATTCTTGAAAAACAAAATAAATTTTACAAATGTTTTTCAATTGTCAACTGGTATTCCTTTGGGCTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATCAAAATGTAGGAGGAGAAACATTTATGTCAAAGGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAAATTAAAAGAATATTTAGAGTCTAAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCCTTATTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGTGTTTGGCCAGGATTAGCAAAAAATAAAACGGTTATTTCAGATAGGTATTGAACATCATCATTTGTTTATCAAGGAGTTTTAGGCGGTTTAGGACTTAAAAAAGTAAAAGATATCAACAATATTGCAACTGAAGAAACAACACCTGATTTAACTATATTCTTTGATATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGATATGTTATTAATCAAGGTAATGAAAAATATGAAATTATAAAATCTGATTGTAATATTGATGAATTATTTAAAAGAGTATTAAATGTATTAAAAGAAAATAAAATAATTTAATTTGATTTTGTCTTTTTATATTTAAGGTTGTAAAAATATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAACAATAAGCTCCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTGTTAGATTAGTTAATGATGAAAAACAAATAAGTTGGCATGAATGTTTGCAAAAAGAATATTGTTTTATTATTGATGGAAGCATAGATGAAATATCGAAAGAAAATTTACAGGAATTTTTAAATCGTGTTTCCAAAAGCTCTCTATTTGCTAATAAGTATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTGAAATTTTTAGAAAATCCTCCATTTAATACAATAATATTATTACATACAATAAAGCCTCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAGTTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATATTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTATGAATTAAAAAATAGTATAAAAAAGGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAAGTTAATTTTAAAATTAAAGGAAGAATATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTACGAAATTAATAACAAGCGCCTAATTGCATACCATAAATTTAATGAAAAAGAAATGACACCTAAAATTATTGAATTATTGCAACAGGGGATGATTATTTCATTGATTTCTGATGCCGGCATGCCTTGTGTTTCTGATCCTGGTTTTGAAGTTATTAAAGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGCGGCCCAACTGCTCTAATACATGCAATAATAAAGGCTAATTTAGACAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCAGGTGCAAGACAAAATCAATTAAAATCCTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAATGCAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAAAAAAAGTTCGGACGCGAACTTTTTATTATTTAATTTTTTAAATTTAGTTGTTATTTTTAGCAATAAATGCATCTAAACGGCTAGCTTTTCTAGAACCATTGTTTTGATGTAAAACGCCCTTTGAAACAGCTTTGTCAATTTCATGGTGAGCATTAGCTTGAAATTCAGCAGCTTTAGGATCTTTATTAACAGCAGCAATTTTTGCTTTCTTAATAGCTGTTTTAACTGATGATTTAACGGCTGAATTACGGATGTTTGCTTTTTCGTTAGATAATATTGCTTTTTGTTTTGATTTAATGTTTGCCATTTTTTGCCTTTCATGCTTAATAATTAGCAATAGTATTTTACAATAAAAAAGCGTTTTATTTAGAAAAATAATATTTTTATTAAAATATTCTATATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTGAACAAGGAACAAGAAATCGTAGCCTATGTGCCACGTAAGTATATGAATACAATAATACTGGATAATTTTTTAAAATCTTCGGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAAAATAAATTTTATTTATGAGGCAAGGAATCAAAATTTTTAATTCAAAAAAATATATTAAATTTATTTGAATTAAATAAGAATTTAAAAATCTTAAAACAAGGTATAGAATTTACTCTAAATAAATATTTAAAAAAAATATTACTGGAACTATCAACTTTATATGTAGAAAAATTTTGTAAATTATTATGCATACCATCTGAGTCAATAAAAGTAAGAATCTTGAAGAAAAATAATGCCTGGGGAACAAATCATTTTTTAAAAAGAACAATAACTTTTTCAGATCGATTGATTTTTTATAGAAAAGAAGTTTTGGAATATGTTGCTTGTCATGAAGTTGTTCATTTTATTTATCAAAACCATTCAGTTGAATTTTGAAATATGGTAAAAAAATATATTCCTAACTTTAAACAAATAAAAAAGGAATTAAAAGATAAAATTTTTATATAAGCAGGAGATTTCATGGAAAATAAAACAAAATTTATAAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCTATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAAGGATTTTTAGATTCATACAATGCACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCCAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTTTTATATTAAAGCCTTATATGTATGACTCCGACTCTTCTATTCAAGGTGACTATTTTAGCAACGAATGAATTTTAACTAAATCAAATGGTAAAAAATCTAAGATACCCAATCAATTAATTAGTTTAAAAAAATCTATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTACATAATTTAAGCACGGAACTAAATATAGAAGATTTGCCATCGTTTCAAGGAATTTGCAATGTTAAAGATTTGTGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTGTTTCAACTATTTTCAATGTTTTAAATAATAAAATTGCAAAATAATTGTAAAAATTTAGTATTTTCAATAATTTTTCAATAAAACAACAATATCTTTTTGTTATTTTAGTGTTTTTGTGTAATAATTTAATTGTAAATATTAATATTTAATTTATTTATAAAATTTTTTTAAAAAAATAACCTTACGTAAGGAGTATCTTAAATATGGCAAAAGAACAAAAAACAGTATTGGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGATTCCACCGTGATGGCGCTTACCGCGGATGCGTAAACATTGATGGTGCCCGTGTTATTGTTGAAAAAGTTGCTGAAAAACAAGTTAAAAATGAAGAAGCTATTGAATTCATCGAAAGAGAACAATTGGTTGAAAAACCTGCAGCAAAAGCTAAGAAAGTTGAAGAACCAAAACAAGAATGTGAATGCTCATGCTCTTGTGATTGTTTAACCGGTATTGAAAACAACAAACATTGAAAAGAATATTGAACAAAGAAATCTTCAAATAACATTGTTTGTTCAGGTATACAAGACCACAAAGGCAGAGAAAAACTATTTATTGGTCTAACAGTGTGTTTATCGCTTCTAACAATCATCAATTTAATCTTGATTATTTTGTTAGCAACAAATGTTATTTAATCCAATAAATTAAAAAATAAAAACTGGAATTCCAGTTTTTATTTTTTAATTTATTTTAAATTCTTTTTTAATTGTTTTAAGTAATTTACAAATTCTTCTTTGCTGTATGTTTTAGAATCATCTTTTCCATATTCTCTAACTGATAAGGTATTTGTTTCAATTTCTTTTTTTCCAATTACTACTATAAATTTGGTCTTTAAAATTTGAGCATCACGAATCTTTTTATTTATTCTTTCTGGTCTTATATCAATATTAACATTAAAATCTTCATCTAAAAATTCGTTATACAAGTCTTGAGCTTTTTCGTTTAATTCATCATTTATAGGAATAATTGTTATTTGTCTTGGACTTAATCAAAATGGCAATACACCTTTTGTTTGTTCAAGTAGTGTTGCAATAAATCTTTCATAAGTTCCAATTAAACCTCTATGAATTAATACTGGTGTTACAAATTGTTCATTACTATCAACAAATTTCATTTCAAATCTTGATGGCAATAAGAAGTCTAATTGTAAGGTTGACATAGTAATAATTCTATTTAGTGCAGTTTTTACTTGAAAATCAACCTTAGGTCCATAAAATGCAGCTTCTCCAATAAATTCTTTATATTTAATATTTAATTCACCTAAAACTTCACGCAAGTCATTTTCTGCTTTATTTCACATTTCATCATTTTGGAAGAATTTTTGAGTATCTTGTGGATCTCTTAATGATAAAGAAATATGATCTATTTCAATATTAAAATCTTTTAATGCTTGCAATATCATTTTATACAAGTGTTTAAATTCTGATTTTATTTGATCTGGTCTAACAAAAATGTGACCTTCTGTTAGATCCATTCCCCTAACTCTTTCAAGTCCACTTAAAGCGCCCGATGCTTCGTAACGATATAACCTTGATTGTTCTGAATATCTAATTGGCAAGTCTTTGTATGAACGTCTTGTTGAATTGAATAATATTATATGGTGAGGGCAGGTCATAGGTCTAGCAACTAATGTTTCGTTATCCATTTTAATTGGGGCAAACATAGTATCTTGGTAATGTGCTCAGTGTCCACTTATTTCATAAAGCTTCTTTTCACCAAAGTGTGGAGTTAGTACTTCTCTAAAACCAAATTTTTTATCAAGTTTTAAAACATAATCTCTAATTGCATTATGAATCTTCATTCCGTCTTCTAATCAAAATGGAAATCCTTGTCCACCTAATTGGTTAAAAGCGAATAAATTTAAATCTCTTCCTATTTTACGATGATCTCTTTCTTTTCTTTCCCTTAAGATATCTAAATATTTTGCTAATTCATCTTTTGTTTCCCAACAAGTACCATAGATTCTTGTTAACATCTTGTTTTTAGAATCACCTCTTCAATAAGCACCGGCAATTCCTAACAATTTGAAGTATTTAATTTCTCTAGAATTTTCAATATGGCCACCTGCACATAAGTCTGTAAATAAAATTTCTTTTGTTTTTGGATTTCAATATTGAAAGAATGTTATTTCTTTTCCTTCTTTTTTAAATTCTTCATAAAGTTCTTTTTTATATGGTTGATTTTCAAAAGAATATTGTTCAATGCCAACTTTTCGCATTTCATAACCTTCTTTTGCTAATTCTTTCATTACTTTTTCAATATTTGGAAGATCTGATTCTAAAATTGGATTTTCAAATTCAAAGTCGTAATAGAACCCTTCTTCAATGGCTGGTCCAATTGCCAATTTAGCATTTGGATAAAGTTTTAATATAGCAGCCGCTAATAAATGGCTTGCTGAATGATTTAATTTTTTATTTGCTTCCATTATCTTAATCCTATTCCTTTTAATAACATATCTAAGTTAATTTGGGCAACTTCACTTGCCTTTTTTGCTCCCTCTTTACTCAATGTTTCAATTTGATTTAAGGCATTTTTATACTTATCTTGGATCTTAATTAAAAATTCCTTTACACATTGAGCGACAGCATTTTTAAATTCACCGTAATTGCTGTTTTTAAATTTTTCAGCGCTTTCTTGTAATGAAATATCATTTAAAGCACTATATATAGTCAAAAGGTTTTTTATGCCCGGCTTTTCATCTGACAAGTATATTTTTCCTTCTGAATCTGTAACAGCTTTTAATATTTTTTTGTATGCATCTTCTGGATCATCTAATAAATAAATTGCTGATTTAGGATTTGTATCAGATTTTGACATTTTCTTACTTGGGTCAGTTAAAGACATTATTTTGGCCCCAACTTCTGGTATATATGGTTCAGGTATTTTGTACGTTGTATTGAATTTATTATTCAATCTAATTGCTAAATTTCTAGTCAATTCAACATGTTGCTTTTGGTCAATACCAACGGGAATAATATCTGCATTATATAGTAATATATCGCCAACCATTAAGGTAGGATACATCAAAATTCCTGTTGGAATTGTTTCCATACCGTTTGCAGTTTTTATTTTAGCTGATTTATCTTTAAATTGCGTCATTCTTGATAATTCTCCGATTGTCGTATTTGTCAAAATTAATCAATTCATTAATCCATGTTGAATGACATCTGATTGAAAAAATAACACACTCTTTTTAGGGTCAATTCCACATGCAATATAAAGTGCAAAAATATCCTTTTTATTTTTTGATAATTGCTTAGGATCAATTGGCAACGTTAGAGCATGTAAATCTGCTATAAAAATGTAATTGTTATATTCTTCTTGCAATTTAACCATATTTTTTATAGCGCCAATATAATTTGCTAGCGTTAATTTACCAGTTGCAGTAATTCCGCTTAATAACGTCTTCTTTTCAGAATTTTGCATAATTAGCCTTTCTTTTATTGTTTAATACAAATAAAATTTTATAACTTTCTAAGATTATAAAATAATTTTATTTTACATTTATAAATTAAATAATATTAATTAATATTAATATATTTTTATTAAAATATAATTATATTACTTGCATAAGTTACATAGGCAAAACTATAATAGGAGAAACAAATGCTAAACAAAAATAAATTTTTATACATATTTTTAATAATCATTACTCTTGGGTTAATCTTAATATTTTGAAGAAAAAAATACAAGGTTTTAAAAACTAAGAATTATTTAACAACAGACAATGAAATAACCTTTTCAATGCAAGATTTAATTTTGTATTTGGGTGGAATTTCAAATATAGTTTCTTCATCAGCAACTCAAAAAATTGTAAGAATTTCATTTAAAAATAAAGCAAATATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCAGGTATTGCATTTCAATCTTCAAATATATCATTAGTTGTTGGAAATTGCGCAAATTATTTATCTACATTACTTAATAAGGAATTAAACAATCATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTATAACAATTTTGGCCAAGAAATATTTGATCAAATTTTCAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAAGAAATAATAACGATTTATAAAGATAATAATGTCAGAATCGAACAATCAATATTTAAGATGCTATCAACTGATTTTGAAAAAGCTAAAACCTTATTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGAAAAAAAATAATAAATAAGGCTGAAAATATAAACATTGAAACAAATATCTGACCTTTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCTTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCCTTGGTTTATTTAAAATCGAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGCTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATCGAATCTATATTTGAGCATTTAAAAAAAATTTGATCTCCTATATACAATATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCTGGATATTTATGAAATCAAATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATCAACACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCTACAGTGTCTGCACTATGTCAAGATACAATAATTTGAGGACCTCCAGGTACCGGGAAATCTCAAACTATTTCTAATATTATTGTCAATATAATTGCAAGGGGATATACTGCACTAGTTGTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAGAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAAAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAGGGCTATGTCGATACATTGAATGAAATAGAAGCACTGCCAAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATTTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATACGAAGTAAATTTAAAAAGAAGACCGTCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATAAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTATTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCGTTTGCAATGTCTATAAGAACAGGTAGCTTAAAACCATATATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGAGGTAAAGAAGAATTTGATTATGCAATCCTAGACGAATCAAGCCAAATCTTTATTGAAAAAGGAATTCCAATTTTATACTTAGCTAAAAGAAAGATACTTGCTGGAGATTCAAAACAAATGCAACCAACACGTTGATTTTCGGTTTCATATAATTTTGAAGATGAAGAAGAATTGGGTGATATTGAATCTCTTTTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTAGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAGACCAATTGATGTAATTCAAGTTGATGGCGAATGAAATAATAGTTACAATGAAGCTGAGGCTAAAGAAGTATTAAGAATAGCAAAAGAAAATCTAATAAAATATGAAAAAATAATTATTCTTGTTTTTAATGTTAAACAACAAGAATATTTGACAAATGCAATATTTAGCGTTGAACCAGAATTGGAAAAAGCCTTATTTTCTGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGACGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGTGGGAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTCAAAGAATGGCTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCAAAATTAAACGAATTATTGAAAGAACTAAATGCTGACAATTTCGAAATTATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGACGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGAATAGTAGAAAACAAACAAATTTTAAATAAGATTAAAATTTTAATTAAACACGAAGAAGAGTTAAATTCTCAAATTTTATTGTCTGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTTCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTAATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAGAGCCGAATACTCAAAGGACTATGAAAAAGATTTTTTGATTAATTATAATGATGTTGAATTGGATAAAACGCATATTGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGTCATAAAGATTGAAGATGTCACTTCATCATCGGCTAACGATGAATTAAAAGTAACTAATTCTAATGAACAAAAAATTTCTGCAAAAACAGCAAGTGAAGATATTGAATTGGGATTTTTAGTTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACGTTCGATGATCTTGATATAAATATATCACCTGAAAGCGAACAACAAGTTGAAGCTAAACTCAATTTGGATTCAGGCATTATTCAAGATAAGTTGCATGAGCAAGACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACAAAAGAATGATGATGTTCAAAGTCCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAACGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGATGAAAACGAAGAAACTTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATACATGAAAAATAAAGATATTGAACAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTCAACGTGGAGGACAACAATGTCAAAAAATAGCGACCCTCTTGATCAATGAGAGCAAGAAATAAAAAGAAGAGTTTCAATAGAAGATCAATTATTGCATAAGGATATTGGAACTAAAATCGCAAAGCAAAAAAAGATTAAAAAAATTTTATTCATAACTCTTTCTGTTGCTGGTATCGTTACAATCGTAGGGGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTATATTGATGTTTCTCTATATTCACAAATAAATTCTAAATTTAAAGAATTGTATCTCGATGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGAAATGTAAACACTAAACCAATTTTAGGTGAGCCTTTTGAAATTCTAAAAAAAATATTAAGTGAGGGTGCCAATTTAAACAATGAAATCTTTAAAGAATTCAATTTTAAATATGATGCTAGTCAATGAAATCCTAGATTAACTAACTTCTTTTTAGATGCAATAAATAGAAAGCATTTGCATTTTGATATTTATTTAGAAAACAAGAAAAATTTAAATCAATCATATGTGGTAAGAAATTTTACTATTGAAACAACGAATGTTTTAGATAATGAAATGTTTTCTGAAACACAAATTGCCAAGTGAAACGAGCAAGATCCTAATATTTCTAAGGCGGCTTTAACTAAAAATTGAAATTTCTATTATGTTACTAAAACAAATGCAAACCTAAATTTTGAAAATGATAAAACATTCGAAGAACTTGGAAACAAAATAAGTAATTTTAATAATAAAATTTTGAATAATAGTGATTACAATTACGTTGATAAATCGCTTTGGTTAACATCTCATCCTAAAAGAACCGATGAATCCGAATTAAATTATTTTAAGTTGTTTCAGTCAGATGTAAGAGACTGGTTTGTTCCTTTTAATTTCTTAAGTCAAGGAATTCAATTGAAAAAGATTGAAAAAAATGAAAATAATATTTTTACTAAGACGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAAAAAAACCGTTTAAGTCGGTTTTTTATTTAACTAAATTAAATGAGAATTTTTTTCTTGCTTTCAAGGTTGATTTTTATTGATTCTGTCTATAAATAATTTTCCTTGCAAGTGGTCATATTCGTGTTGCAATACAATTGCCGGATAGCCGGAAACATCATAAATTTTTTCTTTTTTTTCAAAATACGAATATGCTTTTATTACAACTCTTGAATATCTATATACAAAACCTTCTTGACCTGCATCACTTTCCTTAACACTTAAACATCCCTCCCCTTCTTGCAATGATATTTTATGGTTTGAATGTGAAAGCATTATTGGATTTATTAAAGCATCTCTAAAAATATAATTATTTTGATCGTCTTTTACCATTACATAAAAAATATTTTTCAATATTCCATATTGAACTGCAGCAACTCCTACGGCAGGTCTAAATTTTGTTTGAGGAGTTTGACTATCATCAACATGAAAAATCATTTTTTGTATTAATAAATCATCTTCTGCGGTCAATGGTAGTTCAACGTTCTTTGATTTATTTCTCAATACTTTTTCTGGTAATTGAACTAATTTAACATCTTCAAATTTAAACATAGTTAAATTATTACATATTTAATTATATTTAAATGAACTTTTTAAAAATTTTTATTATAATTAGAGATTATTTTAAATATAGGAGAAATTAATGCCAGAGCCTCTATCCGGTAACTATAATACTTTAAATTCAGCATCAGGAGCTTCAACTTCAACCGGTGCACCATGACAATACATTTTATATTCAATAATTCTTTTTTTATTAATATTATTTAGCGCTATATTTTCAGCTTCAGAAACAGCATATACAACCGTTTCAAGGGCTAAGATTGAAAATATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTGTCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTGCCAAAGCTAATTGCAAAAGCATATCCCGAAAGAGTTATTAGATCAACGTGTTGATTTATTGAAGCTTTATATTGAATATTTTTTCCAATTACTTATCCAATATCTAAAATTGGCAAAAAGATTTATATAACAAATACAGAAGAAGATATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAACGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGCAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCTGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGGAAGGCCAGGGCACAAATGGGGTTCGTCATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATTATTGAAGAAGTTGTAGGCGAAATATACGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGAAAGCGCTTTGAGTGAGATGAAATTTTAAATTTAGAAGCTCCTAATGATGAGGATTTAAATTTATCAAATTGACTAAAAAACCGTGTTGGCCACGAGCTACATGTTAATACAAAATATACTTACGAAGATAAAATTGATTTTCAAGTGATATCATTAAAAAATAAAGATCAAAAATTTGATATTATCGATATAGATTGAAGTAAATAAAACCTGTTTTTCTTAACGGGTTTTTTATATCTTTATTATTTATTTTATAAATAAACATTCATTTTATACAAAAAAACTATTTTGTGTATATAATGTAGAAGTTAAAAATATTTTTAACAAGGTTTATGACTCCTTGACCGTTAGGTCCAAATGTCCAAAAGGAGAAAAATTATAATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTTCAACAAAGGCAGAACCTCATCTAATGTCTGAATTAACAAGAAAGCTTAATATTGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAGTTAAAAAATAATAAGATCGGTAGGAAAAATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGAAGATCTAACGCAACTTTTATTAATAAATATTTAGATAAAGGTTCGCTTCTTCTTGTTGAGGGTACATTTCAATCTTCACGTTTCAATAACGGCGATGGTCAAATTTCAACATCATATGTTGTTAGTGTAGAAAAAATTCAATCACTAGAAACACGTGAAATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGAGATTTATAGGAGTTAAAATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGCAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCGGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAGCAGAATAAAATAATAAAAAAATCAAGTGTATGTATTTGTTATGTAACTTGATTTTTTTATTATAATTTTTAGTAATCATGAATATAAAAACCAAGCAAAAACTAGCAATATTTATTGGCGTTGTCAATTTTTTAACAATCGTAATTTTTTTATGTTGATGTTTAACTTATTTTTTAGAAAAGAAAATAGATTTTAAATTATTTGGTTATATTTGTTTAGGCTTTACATTGGTTGCTTTTTGTGTAAATATAGTTGGATTAATTTACACTACAAAATATGATATTAAAAAACCTTGAAGAGTTTTTTTTATAAGCCTTTTAATAATACCTTTAGGATTTTTTGGGGCAATAAGCTTAGCGTTGAATTATCCAGAAATTAAAAAAAATAGGAGTAAGAGTTAAATTAATATGAATAAAAAAATAAGTTTTAGTCAGATGATCTTAAATTTGGTGGCATTTGTTTGCTTCATCGTACTTATTTGATCTATTGTGGCCAATATTTCCTTTAAATTTACATATGAAAAAAATTTTCAAAATCCAATTTCGGTTTATGAAACTAATAAATTTAATATGATCAATAGAGTCTTAACGACTTTTTTAGTATTGTATATTTTATTTACTATTGCTTCTATTTCACTGAATATTTATGTTTCAATAATTTTATTCAAAAACAAGTATGCAAAACTCTTTTTACTTTCATTAGCAAGTGTTTTAGTTTCAAGAATAATTGGTATTTTTTGTGGAATCATTTTGCTTTTAAATAAAAGACTTTCAACAAGCAAATGCTAAAAGTCTTTTATTTTTTAATAAAATAAAAAAATTAAATATTTTATAATTTAACCATATGGATTCTAACTTAGCAAATTTAATAAGGCCAAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTACGGAAAGCCAGGTACTGGAAAAACAACTATTTCCTATATTCTTGCCAATGCCTTGAAAGTAAATTACGAATATTTTAATGCGGCTATTGAAAATAAAGAAGATCTTGTACATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGCTACCTTATCTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCCGCATTACGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGAGTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTAATTATTTAGCCTTGCAGGCTAATGGCGACTTTAGATCAGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCATTGGAGGCAATTAAAAAGATTATTCCCTCAATTCAATTTAGTTCAGATGAATCAGGTGATAATCATTATGATTATTTAAGTGCTTTTCATAAATCACTACGCGGTTCGGACATTGATGCAGCATTATATTGAGGATTTATCATCATTAAATCTGGAGATTTTGATGGTTTATTTAGAAGAATGTTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCAGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGCTCATCAACATTAATAAAATCTATAATTGATAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATATTGAGATTTAATAAAAAATAAAGGAGAAAAATAAAATGGAATTTGATTTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTCAACAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAGAACTTGGTAAAAAAATTCAACTGTTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACTGGAGGAATTCATCCAATTAATTTGGTTGCAAATAGATTTAGAGAATGATTATTGGCAAATGGATATTTTGAATATACAGGGCCAGAAATAGAAAATGAAGAATATAACTTTGAACGTTTGAATATTCCTCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACGGGATTTAGTGCAAGGGCATTAGAAGAACATAAAAATCAAGAGTTCTCACAATTTGCTATTGGAAAAGTTTATAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGCAACCATTCATTTGGAACATTGATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCTTATTTCCCATTTACTGAACCTAGTGTTGAGGTTGATGTTTTGTTTAATGAAAAATGAGTTGAAGTATTGGGCGCTGGTATGTTGCATGAAAATGTTTTAAAAATGGCTGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGACCAAAGATTTTTAAAACAATTTAAATAAAAGGAGCAAAAATGTTATTTTCTTATAAAAAAATAGTTAAATTGGCAAATATAACTGATAAAAGTGTAGAAGAGGTTGTTGGAGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTTTAGATGCTGAAGGTATGAAATTCTGTCATGTATTAAAAGCATACAAAAATCCTAATTCTGATCGTTTAACGGTTTGTGAAGTTGAATTTGGCAATGGAAATCATTCAATAATTCAAACTAATGCAACAAACATGCATGAGGGCGATTATGTAATGGCTTATGTTCCTGGATCGAGATCTGGAAAAAATATATTTGCTGCCAAGGAATTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCCTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTGAATATTTTGATCTTGATGATTATTTGATTGATGTAACAATTTTATCCAATAGAGCTGATGCTTTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCTTATATTGAACCTCTTTCAAAACCAAAACCTACCTTGGAAAGCAATATTGTTGTAAGCAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCTATTACTAAACAACCTGCTATTTCTCTTCAAGATGAATTTCTTTTATGAAAACACGGAATAAAAACATTTAGAAATGCAGTTGATATAACCAATTTGGTCTTATTATACGCAGGAGTTCCTTGTCACGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGTAATGAAACATTAAATGTTTTAGGCAATAAACAGGTTACATTAAACAACAATTTGGTTGTAAAAAACGGAGATCAAACCGTTTCAATAGCGAGTGTTATAGGCTTAGAAAATTATGAATATAGACCTGATGATGGACATTGCGTCTTTGAATTAGCATCATTTTCATTAAAAGAAGTTAGAAAAAGTATAAAAACAGTCAAATTAGAAACGTTAGCATCTTTACGTGGTTCAAAAGAAATATCAAGCGGTCAAGTAGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCGCCTAAGCTAAGAAAAACAAATATATTAATTGATAGCCATTATATTACTAAATATGCAGGCTTCAATATTACAAAAACAAAAAAATATATTCAAGTTTTGAATCAATTGACTCTTTTGGGCTTTAAAATAAAGGAAGATTTATCATCAGTAATTTTTCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTGTTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAACAAAGAATTTAAGTTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTTAATCCATTTGATTTTAAAACTAATTTCAATGCAATTGCAGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTGGATACAAATATCAAACAAGGCTTGCCGGCTGCTTCTTATTTTGAAATTGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGACATTTGTTCAATAACTAATGAAACATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAACGAATTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGCGAAGTCTTTTTGAATAAAATTAATAACAACGTAATTAAATATAAAAATTACAAGCACGCACCTCTTAAATCTAGGGATGTTACTATATCCGTTTTAGATAAACAATCTATTGAAGAAACAATTAAAAAAATACAAAGTATTTTTGGCGTTTACAAAATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGAGCAACCAAAAAATTTGATTCAATTTTTAATAAATAAAATTAAAAAATGGTAAAAAAAGCCATTTTTTCTTTATTTTTTCTCTATTTTAAATTTTTAGGATATTTTATGAATATACTTAATTGCAGGAGTGAGAATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTCGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCTGAAACTTTTAGCAGTGGTTCGTTGACTATCGATAGAGCGTTAGGAATAAATGGATGACCAAAAGGCAGAATTATTGAAATATATGGTCCAGAAAGTAGTGGAAAAACAACATTAGCGTTGCACGCAATTGCTGAAATACAAAAAACTGGCGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCCGGAGCAATAGGACTAATCGTTGTTGATTCGGTTGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCTTTAAGAAAAATAACTGGATCATTATCAAAAAACAAGACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAATGGAGACATCACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCATATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAAGGCTCGTGATATAGTTTTAATGGCGAAAATATAGCACAAGGGGTATTAAATTTACAATTTATTTTGCAAACTAATAAAGAATTATTCGATAAATTAAAACAAGAAGTTTTAGACAAATTAAATTCGATAAATTAAAATAGAAATTTAAACATATAAATGTTTATAATTTAAACTATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCGGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCGGGAAGAAAGGGTCTTGAGCCTAAAGATTATGAAAGATTAAAAAGAGCGGGAATAAATGCATTTACATTAGGAAATCACGTTTGAGCCAAAGAAAAGATTCATCAAATAATAAACAATGGAGATTTAATAAGACCAGCAAATATAAATAGTTCATATCCTGGTAAAGGCATAAGAATATTTGATATTAAAGGCCATAAAGTCGCAATTATGTCTTTTATGGGCATAACATTTAATCCTCTATTAGAACCATGAAAAGAACAAAGCGCAAATAATTTCTTTGATGAATTTGATGAGTTATATGAAAACAATGGCGCAGATTATTACATAATAGATTTCCATGCTGAGACAACATCTGAAAAAGCGGTATTTTCACTATACGTTGATGGTAAAGTTGATGCAATTTTTGGAACGCATACGCACGTTCAAACAAACGATGCAAGAATTTTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGTCCAAGAGATTGCGCTATTGGTTCTGACTTTCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAAGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACTAAAGATCCGACAGCAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAATATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGAATCTTTCAACACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCCAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATATGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGAGAATGAAATAGAATTTTACCACTATCGAGAGCCATAATAATATACGGTGAATATGAATTATTATTTAATGACCCAAGGGTGGTAATTAATGAGATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAAATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTCGAAGAAACTGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAAACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGCGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCTTGTGATGTTTCATTTATAAGTTTGAAATATGTATTTGAGGTTTGCAATAAATTATTAAATAATAATGTAAATGTAGTTTTTTTAATTAAACCCCAATTTGAGGCTTCTAGCAAATATGTTGAACCTGGAGGGTATGTTAATCAAGAAAATCATAAATATATTACTGATAAAGTAATTTCATATGCGAAAAATAATGGCTTTGTTTTGATTAATTTAATAAAAAGTCCTATTTTAGGAGAAAAATCAAAAAACATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGGTATTGTATATTTCGATAATTCAGCACTCACTTTTAAACCACAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGCGTTTTAGGAATACAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACCATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTTAAAAACAATAATATAACATTTAAATATGCAAAAGACAACGAGGAAGTTCTAGATTTAATAAATGAAAAAACAAGGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTAAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCTTATTTGATAAATGATGCCGCACAGGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGCAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAAAAAAAATAGTTCCTCAAAAATGAGGTGGTGGTCAAGTTCAATCTATTGGTTCTGGTTGCGAATGAAGTGCCAAGAATGATATAAAAAAATTTGAACCTGGAACTCCTAATTTTGCCGGGATTTTTCAATTTAACGCTGCAATTGATTTTATAAATAGTCTAGGTTATGAATTAATAAATAAATTAGAAACAGCTGTTGCAAATTATTGCTATGATGAATTAAAAACAATAGATGGAATAGAAATAGATTCAAAAAGAGGGGATAAAATTATTTTATTTAATATAAAAAATATTCCTTCTCAGGATATTGCATCATATTTAGGACATAATAATATTTATGTAAGATCTGGGGTTTTTTGTGCACATAAATTTCATGAATTAAAACAATATAGCAATAGTTATGTAAGAATTTCAATTTCATTTTATAATGATTATAATGATGTTGATAAATTCATTAAACTTTTAAAAGAAATGGGGGCTAATTTTCTTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGACGATATTAAGCTAAATTTTTTATTTGATGAAAAAAATATTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCTGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATGAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGTGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACCTGATAGACAAGCCTGTTGTAATTGCTAAACGATACAAACGTTCAATAATTAGCGCAATGTCTTACGAGGTAAAAAACCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTGCTTATAGTAGAACCACACTATGATTTATATTCTCTAATTTCTAAAAAAATTTTTGATTATTTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTGTTAGCAATTGGGAGGAAGCTAAAAATCTTGCCCAACAAATTAAAGATGATATTTTAAAAACGTTTATGATTCCTTGTTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTTAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCACAACAATTAATAAAAAACAAAATTTCTACTTATAAAGATTTGTTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATCAATGCAACCAACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGAATGTCAGAGCAAAAAAAGATTGATTTTTTTACAAATGATATTGACATTATTTTTAAAATTGCATTGAAACTTTTTTTAAATTTATGAAATGAAAAAGAATGTTTAAGAGGTCTTGGAATTAGAATCTGCGATTTGAGGTCGATATTTCAAGAAAACAAACAAGTTAATTTGTTTGAAGACAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATACAAAATTTGAGGGTAAAATTTTAATAACAGGGAAACAATATTTGTTAGAGAATTCAATCGATATTGATAATATAAAGTTTTTAAGAAAAAACAATCAACCATATGATAAAAAAATAAATTTAGAGGAGAAAAAATAATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCCGTTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTGGATAAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAGGATAAGCAAGATTATGCATCTAATGAGCATAGAATAAATATGATTAATATTGTTATAAACAAGAGTAATATGGAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAACGTTGGTAGTTTAAATAAATGAAAAGATATAGAAGAAATTTCAAAAATTGTAAAAATTGTTGTATTTAATAGAAATAATATTTATTCAAAAATCAATATTAAAAAATACAATTGTTTAGTTTTAAAAAATTCTTTTCATCCTTTTTCTTCAACTTCTTATAAAAATGGAAATATAAACGTAGTAGATGAAAGGGTTCAAGAATACATAGGAAAGAATTGTTTATATTTAGAAAATATTGCCAAAAATACTCTTTCTATTCCTAGATTTAAACATTTAAGATATACTGCTGAATTTTCTGCACAATTGGCTAAAAGAAATAATTTTGATGTCGAATTAGCGTATAGAACTGGTTTTATGCATGATATTACTAAAGAGTGAGACATAAATAAAGCATATAAATTTTTAAGTTCATATGGGTATAATGAACAAAACTTGCCAGAATATATGCTACACCAAACTACGGCCTATTATTGGTTGAGGGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTGAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGACCGGGAATTCAAAAAATTAGAGAGCTTGCCTTAAATAATTTAGAAGAAGGTTTTAAGGAAGTAATTAGAATATGCGGTCTTAAATTTAATGAACAAAAGGGCATTATTTTTGATGAAAAGCAAAAAGAAATATATAAGTTATTATTAAAATAAAATGTGTTATAATGTATATGCTTTAACTAGCATAAAGTAGATTCATTTCTCACCTTAGTGATTTTTTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATTAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTACCGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAACAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAAAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAACAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACGAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTCCGTGGTAGAGAAAGAGCAAGAATCGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAAACCTTCAATAGCATAAGGAGAATAAAATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAGAAAGGATAAATTATGCGTACTAGAGGCGGAATTGTAACAAGAAGAAGACGTGCAAAATGAATAAAATTAGCTAAAGGATATTGAGGACACAAATCAATAGGATATAAAGTAGCTAAACAAGCAGTTGTTAAATCATGGACATATGCATTTAGAGACAGAAAACAAGTTAAACGTGAATTCAGAAAATTATGAATTGCTCGTATTAACGCAGCTGCAAGACCACTAGGAATTACCTATTCAAGATTAATTGAAGGTCTTAAAGAAGCTAACGTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAAATCTGTAATGGATTTATTTTTTATTATATTTATTAATAATTTATTAATAAATTAATTAAGTTCTTTATAATTTTATTTATATTGCGTAATGGAGGAAGATGAAAAAAAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGCTACAAAAACAAGGATATGATGTTGTTGGTTTGTTCATGAGAAATTGAGATTCATTTTTAAACAATGATATTTTAGGAAATGAAAATATAACTCAAAATATTTGCCCACAAGAGCAAGATTATTTAGATGCAAAGACGGTTGCAAGCCAATTAAATATTCAATTATATAGAATTGATTTTGTTAAGGAATATTGAGAAAATGTATTCAATTACCTAATTGATGAATATAAAAAAGGTAGAACTCCCAACCCTGATATTTTTTGTAATAAATTTATAAAATTTAAAGCTTTTTCTGATTATGCCTTTAAAGAATTAAAAGCCGATTATATTGCTATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAATCTTACTTTTTAGCGCAATTAAGCCCTGACCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTACGAGAAATTGCAAAACAATTAAACTTGTCAACCGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACATTTCACAAAATTTTTAGAAAATTATATTCCTGCTCAACCGGGTAATATCATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCGATGTATTACACTATAGGCCAAAGAAAAGGTTTAAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGATCATATTGACTATTTAATGTCAAATAAATTAATTGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAAATTTTAGATGATAATAAAATAGAAGTTTGCTATCCAAATACCGAAGCAGTAACTCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTCGTTGGTGGAGCAATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAAAGGAAAGGAAAATAGAATGCTAACTTCAAAAGAAATTAGAAAAATGTGATTAGATTTCTTTAAATCTAAAGGACATTTAGTGGTACCTTCAAAATCTTTGATACCACAAAATGACCCTTCTTTGTTATGAATTAACTCAGGAGTTGCAACATTAAAAGATTATTTTAGTGGTAAAAAAATACCTCCTGCTAAAAGAATTACCAATTCTCAAAAATCAATTAGAACAAATGATATTGAAAATGTTGGAATAACAGCAAGACACCATACATTTTTTGAAATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTCATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGAATTTCAAATGGTATTGATCCTTCACATATGATAAAGGGCAGTAGAGATACAAACTTCTGAGATGTTGGCCAAGGACCATGTGGCCCTGATACCGAAATTTTTTATGACCGTGGGACAAAGTATGATACCCGTGGCATTGAATTGCTAGAAAACGATATTGAAAATGATAGATATATTGAAATTTGAAATATCGTATTTTCACAATTTAATAATGATGGTGAAAACAATTACACCGAATTAGCAACAAAGAACATTGATACTGGCGCTGGACTTGAAAGAATAACATCAATCTTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCGACAGAAAAATTAACAAACAAACGTTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTCTAATAAGAAGAGCATATAGAGCCGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATTTATGACATCGATGTTGAAGAAGTTAAAAAAGTTATTTTGGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGCGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACTATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTACAAGAAAAAAACATTGACATCGATATGAATGAATTTAACGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCCGGAATGCAAAAAGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTGGGATATGATCACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGCGAAGAAAAAATAGATAATGCGGGCGAAGATGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGAAATAATAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGAAATCATGTTCATGTTGTAAAAGGAAAAATAAATTCAAATGAACCAGTTCAATGTTTTGTTGATGAAGATAAACGTATTAATTTTGCAAGAAATCACTCTTCAACTCACTTGATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAGCAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCGGTCTTGATCATAGACCAACTAAAGAAGAGATAAAACTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATTAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTATAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTAACTTCATATGATTTGGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAAAAATAATCAAAAATTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAATGCGAAGAACAACTAAGAAATGATTATCGAGCATTATTAAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATTTCATTAAGAGAAGCAAATCCTGATTCAATTATTATTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGTGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAACAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAACCATTTTAACTTAGTTATTGAAAAAGTGCTACATTATTTAAATAAAAGTGAATATCAAGGTTCAATTGGCACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACGTATATGGTCGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAATTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAAAGTGCTGGATGAAACACTAAAAAATCTAAATCAAAGAAAGATTCACTAGCAGCGCAACTTATATTAGAAGATTATTTAAAAGGAATTAAATATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACAATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGATGGAAAATCGGATTTAATGAAGAAATTAATTGGCAACGATCAAGGTGAAAATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACCATTTTAGCACGTAGACATTTCTTAAAAAGTACAATAACATCAAGTGAAGAACGAGAACTACAAAATTTAAAAACAAAAAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGATAATGTTTTAGGCGACCATTTGATTGCGTTTTTAATTAATACAATATATTTGAATAATTTTTTAAACGTTAGTCTTATTGAAAAAAATGACTATGCTTCAATTTCAATAGCAGCATTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCAAGATTTAGATTTATCTAGATTAAATTATGCTGATGAAATTTATAATCCTTCAGATATGATAATATCTTTATGTGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAATGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAATTTAAAAATATTTGCACTGTGCAAATATTTTTTATTGTTATTTTATAAAATAAAATATTTTATTTAATGAAACAATTTCACACATTAATTTATAATATCTAATAATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTATTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAATAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGCGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAGCTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGCAATAAATTAGCCAAAAAATACGATTTAATTGTTGCTAATCCGCCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACACATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGTTAAGAAAATACAATTTTGAACCAAAAAGAATTCAATTAGTTTATCCCAGAATCACTGATTTACCAAAATTCTGTTTAGTTGAAGCTAGGTTTAATTCAGGTTGAGGAACATATTTTGAAAGTAATATTTATTTACATAATGAAGATAAAAATGATCACAGTTATAGCCAAGAAGTAAAAGAATTATATAAACCAAAAAAATCAAATTTTTAAATATAAACATTGAAAGGAAAATATGAATAATAATAAAAAGACATTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTGACTACTACATTAGCTTCAGTATATAGAAATTACAAAAATCAACAAGGATATGAAACCTTGTTTATTACTGGAATTGATGAACATGGTCAAAAGATTCAAAAAAAAGCTCTTGAACAAAATATTACTCCAAAAGAATACGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGAAAATTATTAAAAATTGATTATGATAAATATATTCGTACAACTGACGATTATCATATTGAAACAATTCAAAAAGTTTTTTATTTAATGGAACAAAAAGGCTATATTTATAAATCTAAATATGAAGGTTTATATTCAATTAGCGACGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGATGGAAAATTTTACCACCCAATTTCAGGACATGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACTCCTTGAATAAAGTCATATTTCGAACAAAATCCTGATTTCATTACCAATAAAGCAACATATAAAGAATTAATGAATAATTTTATTAATAAAGGTTTAGAAGATCTTTCAATAACAAGGGTTTCTTTTGATTGGGGAATTAAAATAGAAACTAAAGATGCTGAAAAGCAACACATTATTTATGTTTGATTAGATGCTTTATTTAACTATTTATCAGCATTGGGATACAACAATTACCTAGGCCAAGAGTTATATAATAAATTTTGGGTAAATGGCAATGAAAGAGTTCATTTATTGGCTAAAGAAATTTCAAGATTTCATGCGATATATTGACCTATATTTTTAAAATCTTTGGATCTTAATTTACCAACAAAATTAGTAATTCACTCATGAATTGTAACTCCTGAAGGAAAAATGTCAAAATCAAAAGGCAATGTTATTGAGCCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGATAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTAGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTCCAAACCAATTGAATTCATTAGATAAAAATGTTTATTTAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAAGCTTTAAAAAGAGCAATTGAATTAAGTAAGTTTTTAAATGAATATATCGATAAAAACGAACCTTGAAAATTAAAAGAAAATTTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAACATTTTATTAAATGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTTTAAATTTGATAACAAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAAATTATTGGTAAAATTAGTTCATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGACGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGTGGTCCTGACGGTGGCGACGGTGGCGACGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTTGCTGAATCAGGAGAAAATGGAAGGCCAAAAAATCAATATGGGGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAGGTTTTAGCAGATGTTGGCTTTGTTGGAAAACCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCCGATTTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGCCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGCGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAATAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAATGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGATGCTTGTTTGAAACATTGCAATTTGCTAAAGATTCAATCGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAACCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGCGATGATATAGATAATATATATAATAAGATACCAGTGATTTCATTAGATAATTTATGAAGATTTAATGCCAAATTAAAAGCATTAGGTGTATTTGAATTAATAAAACAAAATAATATTAAAGACGGTTCCACAATAAAAATTCATGATTATGAATTTATTTGGAATGCTGAAGAATTTTAAAATTTGTCAAGATTTTATTATTTTTTAAAAATTGCATATTACTATGTAATAAAAAAAATTTTAATTTTAAAACTTTTAAAAAAATAATATATAATAATTAAGCATTAAGCAAAAGCAATAAGTATTTATTTATAAACAACAATTGTGGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCATTTATGGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCATTATGGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCAAATGCAACAAGCCTAACGGGCTACGGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCATTGGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCATTATATAGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCATGGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCATGGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCATTTCAGAGATGACTAACACCTTAGGGTGTTTTTTTATTTTTTATGTTTAATAAAAGATAAAAAAAGTAAAGTATAATAATACATGTTATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGAGTTTTTTATTATGGAATTTTAGCGTGACTAGTTTTAGGAGTATTTACTTCTAGCGAAAATTCATGATATTTATTCCTATCAAACTTTGCATGTGTTACAATAACATCCTTTACAGTGTTTTACTTGTATTGATATTATGAAAAATTAACTAAAAAAATGTTAATAAGGGCTATCGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGTCTATTTTTTGTTACGGTATATCAAAAGTTAAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCCGTTCTTCCAGGTAATGCACAACTTGCATTTGGACTAATATGTCCCGCCTTTACAAGTGCAGCATTTTTCCCTGGATTAATTGTTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGAATGGCTCTTGTTTATATGCTAAATAATACTTTTTGAATATTGTATTTCTCATTAAATATTGTTTATTCGAAGTTTGGCGTGGATCAAGCAGCTCTTAATAGTATGCCAGGATTTATCGGGGGACTAGTTTGACAATTACTAGCATTAGGAATGTTTGCTTTCCAATTTGGAGTTACATTACACTTTGATATTAAGCACAAAAAACAACAAAAGCAAGCAATGGTTAACGTTATCAATCAAAATTAATCAAATTAAAAGTAGCAAATTACTGATTGCTACTTTTAATTTCATCATTTGCAACCTTAGAATATGCTCTAATTAATTTACTTGCGCCTAATTTTATGCCACCAAAATATCTAACTACAAACAACACACAATTATTTAATTTATTTCTTTCTAGTAAAAAATATAAAGGTTTGCCTGCTGTTCCTGATGGTTCACCATCATCACTCATTCCACCACATTGAACGCCATTTTCAATAACTAAATAAGCATAAACTATATGTCTTGCTTTTTGATGTTGATTTTTTAATTGCTCTTTTAAAAGTTTCACATCATCTTTACTTTTAATATTAAATTTATAAGAAATAAATTTAGACTTTTTAATTTCTAAAATTGCCATAGTTTTATTATAATAAAAATTATTGTATATAATTACTAACATGTTAAAAATTATTTCAAATTATTCAGACAGAATAGATAAATATGTTGCTAATAATAGTGAGATTTCAAGAAACGATATTAAGGAATTGATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAATAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAGGTTGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGCTTAGTTGTTCACCCAGCTCCTGGGCATCATGACAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGTATGGGGGTTGTTCACAGAATCGATAAAGATACTAGCGGCTTATTATTAATTGCTAAAAACAATAAAGCTCATAACTACTTTGCTTCTCTTTTAAAAACACATGAAATTAAAAGGAGCTATTATGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCCGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCGTTAGTTAAATGTAATTTAAAAACTGGTCGTACTCACCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGAGATCCAATTTACGGTCACAAAATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAAAAAAACCTAAATTAAATTGCAATTTAAAATATAATTATTAAATATAAAAGGAGTTTTAATGAAATCAATAAACATAAATGATTATAATTCTACAAATGAATTATATACAAAGAACAAAAAAGTTTTTTGGGCTTGATTCATTGCATGAAGCGTTTTATTTGCAACAATTTTAATATTTGTAATTGCTTCTTTTATTCAATTTGCAGTTGAAAAACAAAACTATTTGAATAAATATGCTGAACTTTTTGGCCAAATCCAAAAATTAAATCCAAATTTTCAAGGAGATGTATTCGCACTAGCCAATAATGAATATGTCAAATCTTTGGCAATGTCAGGCGTTTTACTTGTAATTTTAATTGCGGTTTATGTTTGACATATTGTTTCCATAGCATTGGTTGCTAAAAACAAAGATTTTTCTGCTTATTCAAGAATTTTAACTACATTATATTTATTTGTAATTATTTATGAAATAATAACATTAATTTTTTCTGGATTTCAATCATTTTCATTGGCAATTTGACAAGTAAATAAAATTTTAAATTTAGTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTATTTTTAAAATGCTCTTATGTCGCAAAACTATTTTTAATTTTAAAGAGAAATTTAGCATTTAAGGCATTAAACAACTCAGGCTCAATGGATGATATTTTAAGATCATATTTCTATGGTCAAGAATCTCCTGAACCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGATGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAAATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCTAAAAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAAATCAACAATCAAAACTAGATAATTCCGATGAAGACAACAACGATGGAAGTCAAACCAGCAATTAATTTTTTGTAATTTTATAATTACTATTTAATAAGGAAAAAACAATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATCCTCCTGCTAGTTCAATAAAAAATGGTGATCAAAAATTATGAATTCCTGCATTAGTTTGCATTATATTAATTTTATTTTCTCTATATCTAGCAATAAAATTTTTTGTTAATTTTAAACGTAGTATTGCTAAAACTAAATCTATTTTAAAAGATGATCTTGTTAGTGCTTATGTACAAGGCTTTAATATTAAATCCTCAGCAGTTTTTAACTTTATATTAGCTAACATAATTTCTTTAGTTTCAATTATCATTTCTATAATATCAATAGTTAAACATTTTTCAGCAAGCTCAACGTTAGGTAAGTCTTCGTTTCTAGCGTTGTTTATAGCAGTGATTGCGCTTTCAATTGTTTTAATAATATCAATTAATTTATCACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATCCAAAGAATTAAATGATTTAAATGCCCCTGAAAATATTAAACTGGATATCAAAGCAATGGAATCACACAACCCATTAGCCCCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAGTATTGAATTATTTAAACGTTATAAAAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCTCTTGAAGTAATTGAAGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATCTAGACAACATAAATAATAAAGATCCTGAATCACAAAAAAATTCTCTAATTTTAAAAGAAATTGCTTGAATGAGTGATATGGATAAAAAGGTTAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATATGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGACTTACATATCGTGATTCAGATATTGAAGATCTATTTTATAACACAAATACATCAGTTATTTATACCATTGATAACGGCAAGACAATTTTAGAAAAACCAATGAATGAATTTTTAAATGAATATGGACAATATTTAGAAACTAAATTTTTTGCATCAATTAAAGAAAATGAAGAACTTAAATAAGAGATTGTAATGACTAAGAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCCGTAGCCCTTGTGCCAGCAACTATGGTTTCATGTTCAAAAAAAGATATTGAAGAAATAAAATTGGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAGAAATTTAGAAGTAGAATTTAATAAAATTATAATTTCATTAAAAAAAATGGATATAACACCTGATTTTAATATGGATCAATTTATTCAAGAAATGACTAAAAAATTTAGCGCTGTAGCAAATAATTTTTTGAATATAAAATACAATAAGGCCAAATTAACAAGTGAATATTATATTTCAAAAGTAAAAGCTCAGCTAATTTTGGAGCAATTTAAAAATTCTGGTATTCTTCCAGAAAAAAATGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCGACTATTAAAACCTCTGAAGAAGAGTTAAAAAATTCAATCAGATTTTTAGATGATTTAAACAAAAAATATAATGAAATTTTAAAAAAGCAAGTAATGATTTCAAAATAAAATTTCTTGCTTTTTTTATTTTTTGTTTTTATTTAAAAAAGTTTAATTTTTTTAAAAAACACCGGGTTAGTTGTTGTATAATTGTACAGTATTTATCTATTAAAATACCAAAGACCATATAAAAATACAATATATAATTTAAATTTTAGAAAGGAAAAAATATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCTGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTGCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATTGTTCGTGGAACACAAGATGCTGCCGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAATTATAGTTAGAAATTAGAAAGGAAAATCATATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAACGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGAATTATTCAATATGCAAGATTAAGAAACGATAAAACTATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGATAATAGGTTGGTTGTATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATCATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGAATGGTTCAAGAACAAGAAAGAGGTATAACTATTACCTCAGCTGCTACCACAGCTTATTGAAAAGGAAAAAGATTAAATATCATTGATACTCCAGGACACGTTGATTTTACTATCGAAGTTGAACGTTCGTTAAGAGTATTAGATGGTGCAGTTGCTGTTTTGGATGCCCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCAACAAATTATCATGTTCCAAGAATAGTTTATGTTAATAAAATGGACAAAATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGCGCTAATGCTCACGCTATTCAATTAAATATCGGTGAAGAAAACCATTTTGTTGGAATCATTGATTTAGTTGAAATGAAAGCATATTCATATGATGGTGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGAGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGACATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAGGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGATGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGAAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTGAAAGTAACAACCACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCTGCTGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGCGCACCACAAGTTTCATATCGTGAAACTATTACAAAGACTGCCGATGTTGAAGGTATGCATAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGAATTAAATATGAACCAAACCCAGATGGAGGATTTGTATTTATTGACAAAATCGTTGGCGGTAAGATTCCTAAAGAATATATAAAATCTATTGAAAAGGGACTACGCGAAAAAATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGACAAGTTAGAGATAACGAAACAAGAAACGATGGAGCCCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGATTCGATCACTATGAAAAATTACCACGTAATTTATCAGAAGAAATTATTAAAAAACGTGGTGGAAAATTAGTAGCTGACGAAGATTAATAATATTAAAAAAGATGCTGCATTTTGTAGTATCTTTTTTAATAACTAACTTTAAAAAATTTATATAATTGTTTTATGCCTTCAATTAAAAAAAATACTAATTTAAATGATTATCAATTAAATAATAATAAAAAATTGCTAAAAGCAAGACATCGTCTAACTTTTTGATTTGGTGTAGTGATTTTATGTTTATTTGTTATAACTTTTGTATGTTCATGAATTAGCGATGGACATGAATTAAAAGTTGCTTTTTCTAAATTGAGTCAAGAAACTCGAGATAAATTAATCTTAGCCAACGCCTTGCCTTCTGTTTCGGGTGCAATGTTACAAGTTAGATTTACATTTACCTATATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTATTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTGGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGAGGTTTAATAATACCTTTTGTATTCATTAATCCAAATATATGATATGAAATTCCTTGAAATTGAAAAGTTTTAACCGTTCCAGTTCATTTAATAAATCCCGTTATAGGAACAACATTTTTTATAATAAATAGACGAGAATTGGTAGTCTCTCATAAGACAATGCTTTTAAGTTCAATTTTTATGCTTGGATATTATTTATTTGCTGTCTTAATCTTTTGAATTGGTGTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGACTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGCTATCATGGCAATAATTTGTTTTATAAAATTCTATTAAATTTATTTATCCCAATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCATTTTTAAGAGAAAATAAACTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAATAAGGAAAATCATGACGGCTTTTAATTTAAAAAAATATTTTGAGTTACAAACAATTGAAATTAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGGGGCAAAATTTTTGACGATTTACATGCCTCAAGAGTATTACCCGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTAAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCAAATGACATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGATGATGTATTAAGATTAATTGACAATTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAATCAACCTTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGCTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAACAATATAGATTCTGGTTATGCAAAATTTGAAACATTTCCTGTTTGAAATTTAGCAGTTGATCATCCATTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATAAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGCGATATTCAAGCATTTCCAATTTTAAGTCAAATAATGAACAAAATTCAAAAGCGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGATGAAATTAAACAAGCGGCAAATAAAGAAATAATTCGTCGCTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTGATTTTTCAATTAGACCTTGCATTGAAATTGCTAACAAATTAGCAAATCAATTGAATATGAATATCACAACTTTTACCTTAAACAACAATATAAATATTACAGGTAAGACATCAGCTCTTCTTGGCTCATCATCAGCTGCGTTATTAAATGCATTAAAATATTTAGCAAATATTCCTAAAGAAATCCATTTACTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGCAAAAATCCCCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAACCAATATGCAAAATTAGCCTATGATCAATTAAAAAATTTAGCAGGTATCGAAGCACATTGCAGTGCAATACTTTCTATGCAAGATATTGAAACATACAATCATTTAAATATTAACATTACCCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAGAATATTAAAAAAACATCAAGTTGTATTGTTAACCGCTTGATGTTTTTTAAATATTATTTTATATGAAAATCTAAATCAAAATCGTTTTCTATTGCTTTGCATTCAATATTGAAGGCAGCAAAAATTTTATTTGAAGCTTTAAAATCATCTGAATCAGGCTTATATGCAACTGCATAAACAACTTTAGAAATTTTAGATTGGACAATTAGCTTAGCACAATGGTAGCAAGGTGAATGTGTTACGTATAATATAGATCCCGGTATAATCTTTGAATTTGTAATATTTGCATTTATTATTGCATTTGCTTCGGCATGGACAACGTAAGTATATTTGGAATTTAAGACATCGTTTTGGGTCGCAGGTCTATTCCAAGTGAATAAATCATCATTGTTAACTTCTTTATTTAAATAACTTGTTGGCATACCATTATATCCTAGCGATATTACATAATTATCTGGACTTACAATACAAGCTCCCACCTTAGTTAAAGGATCTTTTGAACGTAATGCACTTAATTTGGCAAGTGACATAAAATAAATATCCCATTTTAAATTATCTTTATGGTTGTTAAAAATTAATTTTCTTGTTTTATTATTCATAATAAAATATTTTAACATAATAAATACAAAAACAGGCCCCTCAAGTCCTGTTTTTAATTATTCAGTGAAATAAGCTATAAGCTCTGTTAAGACATTAACTATGTTAATCCAATTTACTTGAAATTGAATTGCTATAATTATAATAAAAAATTAAAAAAATTTTTATTTTTTGCAATATTTTGTTTTTAATGGCTAATTAATGCAAAAAATACACCTTAATGTTAAAAAAGTATACAAATTTAAAAATAAAAACTTAAATTGTTGTAAAATAAGTCTATAAAAAAAAGAAGGAAAAAGAAAACATGAATCAGAGTTTGAAAAACCAAAGAAACTTAAAGAATATGTTTAAACCAGCTATGTCTGGCTTTTTCGCTATATCAATTATTAGCTTTATTATGAAGAATGTTATGAATTGCGTTATGTCTAGAAACATCAACTATATTATGACAAACATCATTTGAGGAGCGAATAATGACTATCAATTTTTCAACTATAAATAATTTTTTTAACAATCAGTATATTAAGGCAAAAAATAATCTTAAAAACTGAATAATGGCTTTTAGAGGTTCATAGCTTCGTTTAATATTTTTATATTTTGTATTTAAGAATATAAAAAATATTAAAAACATAAATTTATTAAACTAGCTAATATTAATATCAAAAGCCAAATATTTCAAATATTAAGTTTATTATTCAATTATTTAATAAAATTACTAACATTTTTAATTACTTAATGACCTTTAAAATTAAAAATGTTGGTATAAATTACAATAACATTCTTTAAACAATTATTGTTTAAATATTTACAAACAAGATATTTGTATATCAACAAAGATATGCAAAACAAATATATAAAAATTTAAAATTTTAAGGATAAAAATGAAAACTAAATCAAAATTATGATTACTTGGTCTTGGGTCCTTACTAGCAGTAGGAGTACCAGGATTAGTAGCCGCTTCATGTAAAATAGACCCGGCATATGTAAAAGGCGAATTCGTATATGAATTCAACGCAGTAAACAGAGAACCTATATTTGATGCAGACACTTCAAATTCATATGGTCAATATATAAACTCACTACCTGCAACATATACAGGTAGTGAACTTGTTAGAAAACAAGCCTTAACTCAAACAAAAACTATAACAATAACAGAAAAAGACACTAAAAAAACTACTACCTATTTAGTAAAACCTACTTTTGCTAAATATAGATTAGAATTAGCAGAAGCTATTGTTTTAACTGAAAAAGGAACTAATAAACAATATGTATTTGATTCAGATGAAATTCCAAGTGAATCTGAATATCCACAACCAACTAAAACCGTAACAGACGAAAAAGGCAATGAATACAAAGTTTATGAAAAAACAAACGTTTTTGTTAAAACAAAAGGTGACAAGGATGTTGTATCAAGATCAATTAATAGTGCATTCTTTTTAGACAAATTAGCTAATGCCTCAAAATTACAATTTGTAATTAGAAAAGGTGTTAAATGAGTTGATCACGACGGAAAAGACACTGGATATGAAGTTAAAGCAAAAGACTTCTGATATTCATGACTAAGAACATATTCAAGAAACTTAGACTATCGTTTAAGCACCGGTGGATCAAAAGAACTAGATGAAGCATTAAAATCAAAATTAGCTGAAGAAAATTCACCAGTTTATGGTGACCAATGATCATTTGGTAACATATATACTTATGCATTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGTAATTTAAAAGATAGAGAAGCAATTACTTTTGAATCAATTACTGGAAAAGAAAATGATGCTAACATTTCAAGCTTCTTTGAAAGTCAATTTGCATCAGGAACATATGACTTTATGCCAGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAATGGTAATGCTTTATCAAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGAAAGCTTACAATACAAAATTGGTCAATACTGATATGGAACATCTGCTAAATCTACATTATACTCAGGATACTACTACAACAAAGGTATAGAAAACCAAATCCAAAAATTACCAAAGAATACACTATACTGAGATAAGAAATGAGTTGAAGATGATTCAACAATCAAAACAATTGTTTTCAACTTCCAATCAAAAGCAGTTGAACCCGCATTATACAACCAAACAGCATTTAGAAAATATAGCCAAGGAACAATTAGCCAAATTGGTTTTAGTAGTTTAACAGAACCACAAAAGAAAGATGTTTCTTCAGAACCAGAAAAATTTGGATTGAAATTAAATGAACGTTTGAATGTTCAAACTTCAATCTTTGGATTTGTTAGAACACCATTCATTTCTTCAAAATTAGGCAAAAATTACAAATTCAATGAAAACTTTGCTAAATTAGTATATGGATCTTCTTTAGAAGATCTTGACAGTGGAAAAGCAGACACTTCAAAATCATATCTATTTGGTAAAGGTTTGAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGAGATACATATGCAGCCCAAGCATCTTCTGGCCAAGCAACTCACTGACTTGCAAAAGTTCCAGAAGGTTCAAATTTAGGTGGTTCAGATCAAGGTACTGCAAAATTGAATACCATTGTAGACTTCAGAGACCAAGCTAACTCGCTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTTGGTGCTGAATTAGGTAAGGAATTAAGTCCTTCTGAAAACGCCAAAGCTTCAGAAACCGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTAAAGAAAAAATGAAAGAATTACTAGATGAATTCTTTAAAGAACAAAAAATTACCGATCCTGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATTAATGCATGAGAAAAAGTTAAAAACGTTTTTTCTGAACTAGATCCAAGAATTAAAGTTACAGTTAAGAGCTTTGATGACCCAAATGATCCAAACTTCAACGCATATAGATTTGATGGTTTGACAGGTGAAAACTTATTAGCATGATCAGCAGACTATAACGGTGTTGCCTCAACATACGACGGACTTTCATGAGGAGCTGCATTAGTTCCAACATTGGTAAAGGTTGCGCACCAAACAGGCGCTGAAAAAGATAAGTTTGAAAAAGCCTTCCCAGAAATTGCTAAATTGGCTAAAAAATTGTTAGAATATGCAAAAACAAATAAACCAAATCTATCATTGGATTTTGACGAAATTGATCAAATTGACAATAGATATTTAAATTCAAACTTACCTGCAATTTTGGCATCATATAAATTAGAAAAAGATTCTAAAACTGGAAAATATGTAATTGCTAAAAAAGATGTTAAAGGTTCAAAACCAAAACCAATCGAATTCGATTTTGGAAAAGACAGTTCAGGAAAATCAATAGAACCAACCGATCTATATCAATGATCTGCAATATTCTGAGCTAACACATTTAAGAACTTTACAAATGATGAAATTTCTAAGATTGTAGAAGAATTTACAACATTTGTAACCTTAGGATATACTAGTGACGTGTTTGTTGGCCGTGATAAATTTGTTAAAACTTTAATAAACAAACACTACGTTTCACCAACTATTGCAGGATCAGCAGTTGATTCATACCAAGACTGAAGAATAATCAAAGATACTAAAAAATAATTAATTCAATAAAAAATGACAAAATATATATTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTGGTTGCTGCATTTGGGAACAACCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATACTGAAATATTGCATAAACTTGAAATAGAAAACGGATTAAGATATAAAGATAACCCGGACGTTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAATAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATATTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCCGGATATAAGCGCGGAAAATGATTTGATGTGTCTTCAAATGTTTTTGTATTAATATTTGTTGCTATACCATCATTTATATTGGCTCCTATTGTAATAACTATTTCGGTGGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTTGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGTTATGTAACATATACAAGAAATCAAGTTATTACAGTATTGACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCCTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGACAAGTACCAGGAACATCACAAATAATAGTTAATGCCTTCCCTACAGGAGAAATTAATGTTGTTATGTTCTCTACATTATTCTTTACATTTATATCGCTAATTGCCGATATTATTATTGACGTTTCATATGCAATATTAGATCCTAAGATTAAATATTCTACTAATAGCAAGAGAAACTATTGATTATTTTTCTTGGCATATAGAGAACGTTCTAAACTTGCAAAAGATTTGTTTAAATCACAATTAATAAACAAAGCAAGTCAAAATACAACTGTTAAAGGAGATAACTAATATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCGCCAGAAGATAGAATAGTAATCTCTTCTGTTGCAGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCTCCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAAGTTAATGATGTTGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATATTTAAAAATATTTAATGAACAAAAAAACTTCTATTCTCATGTCTTATTAGGAACATCATCAATAGGCTACGATATTTGAACTACTGCATGATATGCAACATGAAGAGCAATTAAAATTGCTTTAATAGTTGTAATATTGGAAGCAATTATTGGGATATCAATAGGAGCATTTTTAGGATTCTATGCTGGAAAATTAATTGATACCATTATAATGCGTGTTATTGAAATATTCCTAGCACCTCCTACATTAATATGAATCTTATTATTTGTTTCAATTATTGGAGCAAATGAATGGTCATTAGTTTTAACGTTAACAATAGTTGGATGACCAGGATTTGTTGGAATAACTAGAATGTTCATTATTACAGTAAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTTGTTCACGCATTACCAACTATAATTGGAAAGATTGCAAATAATATAGTAAGATCAGTACCAGGTGTTATTCTATGAATAGCATCACTGGCATTCCTTGGATTCTTTAAAGAAAAGAAGGATACTAACCTAGGACAAATGTTAATTGAAGCTTCACAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATCGCACTAGGTGTGCATGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAAAATGGCAAAAATTATTAATAAACACGACAATTCAGACAAAAACAACTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATTTACATGTTAGTTTCCCTCAAGGTCATAAGAAAAGTATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGCGAAATAATTGGTCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACCTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGACAATATATAAGGGGACAAAAAATTGGTTATATTCCACAAGACCCCTTAACTAGTTTAAATCCAACAAGAACAGTTGGAAAGCAATTATTAGATGCATTAAATAATAATGAAGATTGAAAAAACTCAACTTATACTGAAAAGAAAAATTATTTAATTGGTTTATTAAAAACATTTGGATTGCGTTCGGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCAGGGGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATCGCTGATGAACCAACAACAGCATTAGACCCAACAGTTCAAGCTAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTACATTTATGTTATGTATGCTGGTAGAATTGTCGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGAGCATTAATTTCGGCCATACCTGAATCAAAAGATGAAAAACTATACACCATTCAAGGAACCCCTCCTGATATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTACGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGATTATTATCTCCTGAAGCACCAAAAATTGAAAAATCAAAAGAATTACAAAAACGTTTAGACTTATTTAAGAAGGCATTTAACAATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATCTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACCGTTGGTTCAACAATACTTAGACTATATGATGATTACAATGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGCATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCTCATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAAGATATTTTTAAAGATTGATTAAATGTTAAAAAGAATTTTAAATACACATTTCAAATCGAAGCAATGAGATTGGAATTACAAAATTATTTAGAAATTAACAAATTAGCTGAACCTTTATTTAAAAAGTGAACAGAAAACCTAAATAAGCTAGAATTCAATTTTGACATTTCTAGAGAAGATAACTTTAGCTTATTCTTTGGATATTTAGAAGAAAAGCAAAACGTTGAAAGCATTATTATTAATAATATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGCCCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTCAATGTTTAAGCAAAACTTCAAAGATTGCTTATGAAGCTTCGCTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAGAATACATTTGCAAACTATATTCATGAATCAAAAAATGAAAAGAGTTTAATTGACATTGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAGTAAAAGGGGTCAAGTATTAGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGATATTAAAAACGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCCAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGTAGATCTAAATATTTAATTTCATATAAAGCTGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTGAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGAGTAGAAACTAATTTAAAAACAGCTGATGCTATGGCTGGAGTTGACGCTAAACATATGAAGTGGGTATGACGTAAAAGATATTTTGATAGTAAAGTTTCATATCCTATTGCAAAATTATTAATTTCTTCAATATTGTATAAAACAATAATTTATAAAGCATTGGAAGACGTTGGATTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCGCAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTTGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTCTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGTGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGAACACATAATGCAAAATTAGAGAAAGAACATAAAGAATTATAAAAATAATTTATCATTAAACTAAATAAAAAAATTAGACTCTTGACTAAAAACTTAAGAGTCTAATTTTTTTATTTCTTTATATAAGCTAATAATTTATTAATTTCGCTTGAGGTTAAATCTCTATATTGACCTATTTTTAAATTACTATCATCAATGCATCCAAAAGATTTTCTATGTAATTTTTTAACATAATTATTTACTAACAAAAATAGATTTTTAACGTGATGGTTTCTGCCTTCATATAGTAGAACTTCATATTTAAAAGCACTTAATTTTGTAACTTTTTGTTTTGAAATTTTGCCATTTAATAATACGTGATTTGAATTTAATAAGTTTAATTCGCCATCAAGCATTTCACGTTCTAATGTAGCAATATAAATTCTAGGAATTTCAGAAGAAGGATGGCTTAATAAATTAGCAAATTCTCCATCGTTTGTGATTAATATAATTCCTGTTGTATTAAAGTCTAATCTTCCTATTGAAAATAAGCGTTCTTTTGCTTTAAGATATTCGTAAATTGTTTTTCTATTTTGAGGATCACTTGCTGTACAAATTGTATTCATGGGTTTATTTAAAACAATGTATTTAAAATTATTTGCAACATTATTATTTAATTTGATAATTCTATTGTTTATTTTAATTTCGTCATTTATAGTTACACATTCGCCTAATTTTGCAATTTTATTATTTATTAAAATCTTCCCCGATAAAATTAATTCTTCTGCTTTTCTTCTAGAACAAATGCCTTGCTCACTTAAAATTTTTTGAATTCGTTTTGCTTTTTCTTCCATTTTTAAAACCTTACTAAAAAGATATTTATATATAAATATATCTATTGTCGAGTGCTAAAATTATAACTTTTTTTTGAATTTATTTTTTAATTGGAGTTGGAGGAAAAATGGAATATTTACAAAATATAGACTTAAGCAAGATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGTTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGATATTTGGCATATTGATCAAAGAATGCATATTACAAAAAACTTAATTATATAGTTAATTTATTTATTGATTTTATTAACCAATAATGTTTCCTATTGTTATGCAGGCAATCAATGATCAAGAACCAACAAAATGGATTGAAAACATAAATGCTTATACAACCAAACAATTATATTTGGATAAATGAACAAATTCATTTCAAAAGGTTGATTTTAAATTACAAAGGTTAATTGAAAATAATAAATTTATAATTGAATTTAAATATTTGAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGATAAACATGAATATACATATACTCTTATTTCTGAATTTAATGAGGCAACAAAAACTAAAAAAGTTCAGGATTTAAAAAATATTAATTTTGAAATTTTTTATAAGGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAGATATATAGCAATAACAATTCAAACAGAGAACTAGTTTCAAATAAAAATGGCGTTGAATATGATTTATGAACTAAATTAAGAATTCTATCATTAGCAGATAGAGATATTCCGACAGATATGGAACATTATTCATTTGTTGAACAAGTTAAATACAAATTTATGCCAACTACTCTAATGTTGGGAAAACATAATAAAAAATTATTTGATGTTGAAATATCAAAAAAAGAAATTTCTAACAAAAGTGGCATAATAAGCGATTTTTCTCAACTAATTCAAAGCGATATTAATGGTTTTAATGTATCTGTTGATATTGATAAACAATTTATAAAAGGCAACTTGAATGCAAAAATTAATGATTTCAGTAAAAATGAGCATAAGCAAATTTCAATAGATTCTTATTCATATTATGACAAGGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGCGCAGCTAAAGGCTTATTGTTGCCTCTTAATTATTCAGGAAATTATTCTTATTTACTAAATATTAATTTTGGTGAGGATTTGAAAGGTTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAGTAAAATTAAATGTAAATAATATATTTAAACAACAAAATTTCATAAAATGAAGTGTCGTAAAAGCAGAGGATATAAGAAGTTTGATTAATAATTCCAATACTCTAGAAGAATTAATGAAGGGAGGGAAATAATGTTGATTGCAAAAAAGAAAAAAATAATTTTCGGTTTATCTCTTGGATTAGCTTGTGCCGTTGGCGCTGGCGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACATCAATGACAACAAAAACATTAATTCTGGCAATGAAAACGAAAAAGATAACACTATTGATGGCCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGCCAAGCGAATGATGATGATAAAAAGCTTAAGCCAGAAGCAAAGCCAAGGCCGAATATAAAAACAGAGATTAATTGAAGAGAAATATTTCCTGATGTTAAAACTTCAGATTATTACAATCAATTAAATTTTAAAGATGGCCAAGGATTTATTGACAAAGAAATGATTACTTATATAATAAAGGATATTTTAAATAGGATGACTATTTTAGATGGCAATGTTTCTTATGCTTATAAACAAGAGGATGATCAAAATTTATATATTGCTTTTAAATGAGAAAATAAAAGTCAAAAATCTAATATAACTTATAAATTTAGTGTTAATAGAATATAATTAATTTTATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAATTTAATAAAGAAACTTTTATTCGTGATTTAAATATTGATAGTTTAGATTTGGTTATACTTGTTAGTGATTTAGAAGAGGCTTACAATATAAAAATAAGTGATGAAGAATTGCTTTCATTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAAAGCAAAAATAATGTAAAATTATATTGCTTTATATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCATATCAGAAATGATTAATAAAAGTAGCAAAATTTTGCTACTTTTTTTAATTTAAATTATAAAAAAAGTAGGGATTTTAAATCCCTACACGACATAAAAAAATGATATGGTGCCGTCAATTGGAATTGAACCAACGACCCCTTCCTTACCATGGAAGTGCTCTGCCCCTGAGCTATGACGGCATTAAAAGCAAAATTATTATAACAAAAAAACTAAGTTTTTGCTTATAATAATAAAAATAAGTTATTAATAATATTAATAAATTAATTTAAATTTAATTATAGAGGCTATATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATACGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAGATTTATGATGCTTTTACAAAATATCAAAACATTGAAGAACTATATTTCTTGTCAAAAGAAATTTTAGCAAATGAAAAAGATCAAGAATTAATTGAGCTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGCGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATTAAAGGTTTTAAAATCAAGATAATTGATTCTACATATGGCGCAGCCGGAGGATATAGCCAAATTGTTTTTAGTATAAAAGGCGAAAAAGTTTTTTCACAACTAAAATTTGAACGTGGTGTACATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGACGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCCGAAGTTGATGAAGACGTAAAAATCGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGCTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCGACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCCTTAAAAGTTTTAAAAAGTAGATTGTATGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAACTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTATCCTCAAGATAGAATTACAGATCATAGAATTGGCTTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATCATTGAAGCAGGAAATTAATGATAGATAAAGAAATTCTACTACGCGAAAAAGAAAGATATAATTTACCCCTTTATATTTCAAAAAAAGAATTAAGAATGCTTAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTGAATTTAAAATATAAAACCTTGATTCCTAGATACGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTAGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGACGCTAATGTTACATTAAGTGATATTAGCCGTTTTGCAATCAAACAAACAAAAATAAATGCCAAATTAAATAATTTAAATGTAAATGTAATTCGTTCAAATTGATTTAATAAAATAACATCTAAATTTGATGTAATAGTTTGCAATCCTCCTTATTTAAAAAAACAAAAGAAATTAATAAAATCATTAAAATGAGAACCCAAAAGAGCCTTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCATTTAACTATTTAAATGAAAAAGGAATAATTATTTTTGAAGTCGACCCTTATTGCTCTTTATGGTTTAAAAATAAATTTCCAAATGTACTAATATTAAAAGATATAAACAATAAAGATAGAATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAGCACTCTTTTTAATTTTGTGCTAAAATTAAAAATTATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATACCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATATGAAGTTTTATCTGACCCTGAAAAAAGACAAAAATATGATAAGTTCGGACATTCTGCATTCGACCAAAGTTCATTCGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCGAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGTTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTGTAGATTCAATATTTGGAAAGAATGTAACTGTTCAATTAAAGAAATATAGCGAATGTTCACATTGTCATGGTTCAAGAGCGGAAAGCGATAGTGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTCTTTTCAACAATTAGTCCTTGCAACAAATGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGATATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGCGGAATTAATAATGGCGAATATATTAAATTGTCAGGATATGGTAATCCTTCTCGCAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACCATTAATGTTCCTACACCATACGGAAATAAAGATATAAAATTAAATAAAAACATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATTACATTAAAACCTTATATTCCTTCGCTTAACAAAGAAGATAACAAAAAAGTTCAAGAAATATTTAGCAACTATAAAGATGTCGAATTTCAAAAATGAATAAAAGATTTTAATAATTAAATTTAAAAATGCATTTATTAATTTAAATAATTAATTGCATTTTTTTATATTCGAATAATTAAACTTGTATAATATTTAATTATTTTTTACTTTTTAATAAAAATAATAAATCAATGTATCCAAAAGAATATATTCAAAATAGCAAAATTATTTTTCAAAGGAGCAATATGGAAAATAAAATTATGATTGTTGAATCGCCAAATAAAGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAGGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATTGGAGGATTAGGTATAGATTTTGAAAATTGAGAACCTAAGATGGTAATTGATCCAAGTAAGAGAAAAATAGTATCCGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGCGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAAATAAATATAAAAGAGTGAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGATAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATTGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTATTCCAACATTATATTCAAAAATTGAAGCACATACTGCTGATGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGTGAAAATGAATGAATTAAAAGAGAAAAAGTAGATGATATATTAGCTCAATTAAATCAAAATAAATCATTGCAGGTAATAGACTTTAAAAAATCTATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTGTATAAAGAATCTCAATATAGTTCGGCAACGGTTCAAATTGCTGCACAAAAACTATTTGAAATGGGGTTAATTTCTTATCCCAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCTCAAAAATATGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTGCTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACACCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTTAAAATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTATTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTAGAATTTGTTAAATTAGACAAAGAAACTAAGGCTCCTGCAAGATATAATGATGGTTCATTAATTAAGATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACGACAGTTAAAATTATAAAAGATAGACTATTTGTTGAAACTAAAAATAATAGTTTATATCCTACAGATTATGGAAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATTAATGAAGAATATACAGCTAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGGTTATCAGATTATAAAGTTTTATTGAAAGATTTTTGAAATAATTTTAATTTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTCTTGCCTCAAGAAATTTTGCAAGAAAAATGTCCTCTTTGTGGACACGACTTACTTTATAGATATACTAAATCAAAACACCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATTGACAATGAAAAGCCTAAATTTTTTAAAAGAAGATACAATAAATAATTTTTATTTTTAAACTTTACACAAAAAACAAATGTATTAAAAAATTAGATTTTATTACAAATCTAATTTTTTATTATTTATCTTTATTGAGAAATTATTTTAATTTATTCCCGAAATTCTATCAATGATTATAGGATTATAATTTTGGCCCTTGTCATTGCTTCCTAATTTGTATTCTCAGCTAAATGTCTTTTTGTAATTTTCTCCTTTTGTTGAAATAACAAGATTATATTCATAGTTTGAGTATATTGATACAATACCTACAAATCTATATCTATTTTTTGCACCATCCCATTTTGCTAATCCTTTGAAATTTTTTTGTCATCTACCTGGAACATTTTCGGTGCTGAATTCAAATGTAACATCTTTATAGCCCTTAAAAATTTTGTCTTTATTTCTTAAGTCTAAATCGTTAGCATCTGCTCTTCCGGTTTTTGAATAAATATCAATATTGTAAACATTCAATTTTCTATTAATGGTTCCGTAATATTCTTCAGTTTTATGACTAAATAAATTTTCACATTTTTTAATAAAACTAGTTTGAAGAGTTCTAACAAATGCATTTTCTAATATATCATCAAATGCACTTCACTTTACAGCAGGCCCTTCTAATTCATTTTGTTTTCTAATTTCTTCAAGATATTTTGGATCGTTATAATTTGGATTACCATCAGGTTTTAATGGCGCATTACCTTTGTTGTAGAATGTATTATAAAATTCTTCAAAATGAGGCATATTCATTTTTTCTAATTGATCAAACACTTTTTGATCAACTAAATTATTGCTTAAATTATCAAAAAATTCATTTTTTTCTGTTTGGTTATTTTGTGTTGATGTATCATCGAAAAATTTATATGCAGCATTCAAAATTCCTTCATATCTTGAAATTAAAGCCTTGTCTAAAAAGTTTATTTTGTTAATGCATTCATCTTTTGCTTTATTGTCAGCATTTTTATTAATTAATTCTTTTTCCATTTCGGTTATGAAGCCTTGGAAAATTGTCTTTATATTTTTATTTTTGTTTGAAAGACTACCTGAGGTGCCAATGTTTTCAGTATCTAAAAATTTTAACTTTTCTTCAGTTTTTTGTTTTTGGGAATTGAATTCTCTAATATTATTTATTATTCTGCCAAACTCGTTTTTAGCAGAATCTAATTTAGTTTTAGCATTTTCTATATCTTGAATTTTTGAATTTTTATTTATGCTTGAAACACCTTTTATTTTATCTTCAATTTTTGTTCTAATTGAATTATATTTTTGATCCTTCAAATCTTTATTAATAAAACTTTGCAAATCGTTCTTTATTGATTCAAATTTAGAAAATTCATTGCTTTTCTTTTCTTAAATTTTAGTTTCTATTATTTTTTTAATACCATTTATTTTTTGGGTTGCCTGTTTAATTTCTTCATTTTTGGAAGCATCCGATATATTTTTATAATGGGTTAAAATTGTATCTGCTTCTGTAGTTCCTACTTTTTTAGCATCATCAGTTTTTATCAAATCTTCTAATTGTTTTTTGCTGTTGTAAAAATCAGTAATATCTTTTTTTGTAATCTTTGCATTATTTAATTCATTTTGCAATGATTGATATGCATCTTGAATTTCTTTTTTATTATTAGAAAAAGTTATTGAATCTTTAGCAATTTTTTTATTTTTTAAGTTTTTAACTATATTTAGATAATTGTTTTTGGTTTGATGATCATTTTCAATCTGTTTTATAAATTCATCAATATCTTTTCTTGTTTGTTCTAACTTTTTATATTCTTTAAATTTTTCAAACGCTTCTTTTGAATAAGAAATTTTTGATTCTAATTTATTATTTTCATCGCTAATTATTTTTGCGTCATCAATTTTAATATTTTTAGCATCTTCTATTGCTGAATTTAAATCATTCTTTATTGTCGAAAGTATATGATCATTATTATTTTTCAATTCCGATTCTAATTCTTTTTTAAAAGATTCAGCTTTTTCAATAGTTTTATGCAATTTTGTTAATTCACGGTGTTTTTCCACTTCCTTGATTGCATTAGCTTTTGTGTGTTTTAATGTCGATATTGATGTTTCAATTTCTTCTATTGTCGAATTTTCATTTATTGTTCTTACATTATTAAATATATCTAAAACTTTTTGAATACTATCAAAGAATTTAATTGGATCCAAACCATCTAATGAAGAAACATCAACATCTTGTTGATAATTTTCCAAATCTTCTTTTGCCTTATTGAAATCTTTTAATAATTTATCTTTCTTATCTTTAATTTTTTGCTCTAAATTAGTTTTGGCATCATTCAATTCTTTTGTTGCGTTTTTGATTGTTTCTTTATCTGTGGCATCATTTATGGTTTTGTGTTTGTCTAACAACTTTTGGGTTTCATTAATTCCTATTTTTTTAGCATCTGGTTTTGATATTAATGTTTCCAAATCTTTTTTAGCAGCATAAAACTTATCAATTTCTGATTTTTGTTCTTTTGCTTCTTTTAAAGAATTTTTAAGTTCAGTATTAGCATTTACTATGTCTTTTTTATCAGAATTTTTATTTATTGCGTTTTTTTCAGTCTTTTTATTCTTTAATTTATTAATTATATTCGAATAATTGCCTTTAGTTTTAGGATCCTTTTCAATTTGACTTATAAAATTATCAATTTCTTTTTTAGTTTCATTGAAATTTTTAAATTCTTTATTTTTTGATTCTATGAATTTATCAAGTTTTTCTTGAATTTCATTAGTTTTCTTTGCTAATGAATCATTTGAATCAGTCAATGATTTTGTATTTTTTGAAGTAATTGCATTATTTGCTTTTTCTATTTCTTTTTCAAGTTGTTTTTTATAATTTTGAATATCAGCATCTGAATCAACTAATTTTTTTAATAATTCATTTGCAGTAGATAAACTGGCGGTTAATTTATTATTAGCTTCGCCATTTGCTCTATCTGCTTGTTCTTTTGCTACTTTTGCTTCTTGTAATGCCTTAGTTAATTCATTATTTGCATTGATAATATCTGCTTTGTTTGAAGTGTTGTTAATTTTTTCTTGTGTTTTTAAAGAATTTTGTAATTTTTTAACAAGTTCAGCATAATTTGGATTGTTTTTAACATTATCTGTTAAGAAATTATTAATATTATTCCTTGCTTTTTCTAATTCTTTAAATTTAGCATCTTTATCTTTGTTAAACTTGTCAAGACTTGATTTAGATTCATCAATTTTGTTTGCTAATTGTTTTTTTGCTTCAATAATTGCCTTAGTATCTTTTTTAAGTGCTTCTTTAGCATTAGCAATTTCTTTTTCCAAATTAGTTTTTAGCGTAGTTATTGTAGAATCACTATCAATTAATTTTTCTTTAAGTTTATCTGCCTCTTGAATTTTTGCAGTAAGTTGTTCTTTGGCATCAATTTTTGCTTTTTCTTGTTCAGCGTTAGTTTTAGCGTTGATTAATGCAGCTGTTGCATCTTTAATTTCTTGAATTTCTTTAGTTGTTAAAGATTCTTCTTTTATTGTACTAAAACTATCTATCTTATTTTGCGCTTCTTTTTTAATTGAATCGTATTTTTGATTATTTAATTCATCATTAATGTATTTTTCTAATTCTTTTTTTGCTTTATCAAATTTTGAATATTCTTGTTTTTTAGCATCTGAGATTTCTTTATTTATATTATCTATTAATTTTTTAATTTCTTGTGATTTTTCTTGAATTTTATTCTTACTTAATGTTTCTAAATTCAAGACTTCATATTCTTGTTTTTTATTATTGAATTTAGTTTTTTTAGATTCTTCTAATTGTTTAAAATTATTAGAATTTGTCAAAGAATTTAATTCGGTTTTTTGCTTATTGTAATTGTCTATAGATTCTGTTCTTTGAGTTGAATAATTATCCAACGAACCTAATAGTTTTCTTTGTGTTTCATCGATACTATTTTTTGCTGCTAAAATATCAGAAATTTTTGAATTTTCGTTTATTTTGTGTTTTTTAATTTCTTCATCAATTTGTTTTTTTATTGATTTTATTTCATCATCGATATTAATAAATTTATCTAAACCTTTGGTTTTGATTATGTTTTCTAGATGTTCTTTTTCTGTTTTAAATTCTCCGAATTTAGCTTTTTTATCTTCTTTTATTTTTTTAATATCTTCAGCTAATTTGTCTATTGCTGTTTGTAATTTTTGGTTAACTTGTTCAATATTTTCAATAGTTGAATTATTGTTTATGGCCTTAGCGTCAGCAAAAGCTTGATCTTCAGAAGATAAATCAATATTTATGGATTTTGCATCATCTTTAAGATTTGCAGTTTTTAATAAATCTTCAAGTTTTGACTTCAATTCATAAAACTTATCTAATATTGCCTTGTTGCTTCCAATTTCTGTAAATTTTGCATTTAAATTATTTGTAGCTTCTTTAATTTTTGAAATTTTGTCGTTTGATGTAATGTTTTTATATTCATCAATAACATTATTAAATTTATCTTGAATAATTTTATATTTTGAATCTTTAATATCGTTTGTAATGTAGTTTTGCAATTCTGTTTTAGATGATTCAAATGCATTAAACAACTCTTGTTTTTTGTTTTTAATTTCTTCTTTTAATTTTGTTTTTGCAGTTGTTAAATCATCAGTAGCTTTTTTAATTTCAGTGATTTCGTGTGAATCATCTATGCTACTGAATTTTGCTAAAGCTTGTTTAGCATCGTTTAAATTTGAAACCAATTCATCACTTTTAGTATCTTCATCATTTTCAATAAATTCTTCTAATTCTTTTTTAGCTGAAACAAATTTATCAATTTCTTGTTTTTTATCATTTGCTTCTTTTAAAGAATTTTTTAGCAAAGTATTAGCGTCTTCAATTTCCAATTTATTTGAAGATTCAGAAACTGAATTTTTTTCATTTTTAGTCGTGGTTAATTTTGAAATTATGTTTGAATAAGTTGAATTATTTTTATTTGAATTAATAAATTCATCAATGTCTTTTCTTGTTTTTTCTAATTCTTTAAATTTAGCATCTTTATCTTTATTAAATGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAGAGATTCTTTTGCTGATTGCATTGATGTTGGGTTATTTAAATTAAGCGCTTGATTTGCACTTTGAATTTCTTGATTTAATTCAGTTTTTGCTTTTTGAATGTCTTTGTCTGAATCTATTAATTTATTTAATAATTGTTTTGCATTGTCAATTGAATCGCTTAATTGTTCTTTAATTGATTTATTTGCATCATCAATTTGTTCTTTTGCTACTTTTGCCTTATCAAGGGCTTGTTTTAATGCTTCATTTGCAGCAATAATGTCTGATTTATTTGAAGATTTAGTTACTGATTTTTTAGCATCTTTTGCATTGGTTAAATTCTTAACTAAAGTTGCATAATTTGGATTATTTTTAACGTCATCTGTTAAGAAATTGTCAATATTCTTTCTTGCTTGTTCTAATTCTTTAAATTTAGCATCTTTATCTTTATTGAACTCAGTTAATTGATTTTGAATTTTAGTAACTTTGTCATCTAATGAAGATTTAGCAGATTGCATCGAAGCATTGTCATTTTGGTTAACTGCTTTATTTGCATTGGTAATTTCTGCATTTAATGATTCTTTAGCTTTTACAATTTCACTATCATTATCATTTAATTGAGGCAATAATGTGTTTGCTTTGTCAATTAATGCATTCAATTGTTCTTTAATTGATTTATTTGCTTCATCAACTTGATCTTTTGCTTTATTAGCATCTGCTAATGCTTGTATTAATGCTTCATTTGCAGCAATAATGTCTGATTTATTTGAAGATTTAGTTACTGATTTCTTATCATCTTTTGCATTGGTTAAATCTTTAACTAAAGTTGCATAATTTGGATTATTTTTAACGTCATCTGTTAAGAAATTGTCAATATTCTTTCTTGTTTGTTCTAATTCATTGAATTTTACTTCTTTTTCTTGATTGAATTTATTTAAGATTTCTTTATATTCAATAAGTTTAGTATTCAATAAATCATTGGCTGATTCCATTGATTCTTTGTCTTTTGAAAGAGATTGTTCTGCTTTAGAAATTTCATTATCTAAATTAGATTTTAAATCAACTATTTTTTGGTCATTGTCAATTAATAACTTTTTAAATTCTTTGGCATTATTTATAGTTTCTTTAAGAGTATCTTTTATATTTTGACCATCTAATTTTTCTTTTTCAAGGCCTGCTTCTTCTTTTGCCTTTATTAAAGCATTTTGTCCAGCTTTTATTTCTTTAATTGTTGAATTTTTATTTATTCCATTTAAACTATTTATTTTATCTAAAGCCTTTTGTTTTATTGATTGATATTTTGCATCATTTAATTTTGTGTTAATAAAATCTTGTAAGTCTTTTTTAATATTTTCAAATTCTTGAAGTTCTTGTTGTTTGGTATTTAAAATTTTTGATGTTAAAGAAGAAGTTGCTTTTTCAACTTCAATAATTTTGTTTTTTATATTTTCAATTGATGATGTTTCACTTATATTTGTTTCGATATAAACTTTTTGTTCTTTTATAGAATCTACTTCTTTTGCATCTTCTAAATCAAGAACTCTTTTTAATTCTTGTTTCTTATCATTAAAAATTTTTGATTGTAATTCTTTTTCAGTATTTATTTTATTTAGAAGTTCTTTTTTTGCATTTTCAAGATTTTCCTTTGTCTTTTTAATTTTATCTATTGGCAAAGAATCATCTATTTGATTTTCATTTAATGCTTTTTTAGCATCAGAAGAATCGACGTTTTGACCGTCATTTGAATTAATTAAGTTTTGTAAATCTAATCTTAATTTATTTAATTTATTTAATTCTTCTAATCTTTGTTTTTCATCTTTTTGTAGATTTTCTAATATTTTTTTACTAAAAATTTCTATTTCATCAATAAGTTTTGATGTGTTTGAATGATTTTTAAATGCTGTACTATTAAATTTTTTATCTTGTCTTGCAATAAAATCATTTGCTTTCTTTTGACCTTCATTATCTAGTTTCTTAATTAGATTAAGTAATCTAGATTTTGCTTCGTTATACTTTTTCGTGGAGTTATTGCTTATTGAACACATAGCAGCAATAGCTGACGTTGATGCAGCAGCACAAACGATTGAAATTGACATTAAAATGTTTATTCTTTTTTTCATAATTTTTTATTATCCTTTCAAAAGTTAATATTTAAATAACAGAAATAATAGTTATTATTATATTTAATAGCTAAAGAATTTGAAATTATACAATTAATTAGTATTTTTTTAAACCATTAGGTGCATTAAATCTGTTTTACAAAAAATATTTTAAATTAAGGAATATTTTTTATTCTTTTAACTACTAAAAATATGTAAAAAAAATATTGTTTTAATTTTTGAAATAGAAAAAATACTTATAAAAGCATAATAAAATAATGGTTTTAATATAAAATTAAGTAAATATGAAAAATCATTCTAATACTAGACGAAAGCATAGATCATTGGCGCCCTTATATTATACCCTTGGTTCAATTGCAGCCGCTGCTACAATTGCGGGGGCTATATATTTTGTAGTAAAAAACGGTAGAAGAGTTAGCAAGCAATATTGAAGTGTAGAAAAATTTTTGCAAATTAGTAAAAATATAAAAATTGATCCGGTTGTAATTAATTCAGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACACCCTGAGTTGAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCTGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGACGCTCATAATTTCATCTTTTCAAAAATTTTAACAAATCAAACATTTACTGAAATGAAATTCTTAAAAATGAGATTTTCTAATTTGGAATTAGACACATCTGATAAAGAATCAAATAAACTAAAGGTAACATATGAAGTTTATTTAAATTATGAATATGCAACGGGAAGCTTCGAACTTAATAACGTTAAGGGCACTTCGCAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCTGAAAAGTGAAGTTTGAATTCTAAATTTGCTAATAATCTAGAAAAAAATTTAGACGATGCTAGAAAGATTATAAGAATTGGAATAGTTAAGAATGATCCAAAGTGAACACAATCAAATGAATTTAGAGAAATGATTTTCAAATGATTTAAAAGAATTTTTATAGAAAATGATGCCTTTCCTGACACATATAGCAAAGATGAATATGAAATAATGCCTTTTAATGAAACAAGTAGCGGTGATGACTTTGTTAGATGAAATCCTAAGAAAGGATTAAATAACCTAACAATTAAGTTTTTTTATGTAAATAAAAAAAATCCAGATATTAAATCACCTGGCAGGATTAAGATTTTTACTATATTAAAGGTTTAGAATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTGAAAACCCTTTATTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCCAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTACAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAATGTGTCTATAATTAGTATTAATTTAGTTATATGCATCAACACGCTACAAATGTAAAGAAAGTAGGTTATTATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAACAAACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGAAATATAGAAGATATTCATGTAACAGAAGACGATAAAATTATTTTTAGAGGTCAAGATGTTAGTAAATTAATTAGGCAAGAAGAAGTTTCTATGGCCGCATCAGCAATAGCAAAATTTCCTTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATATAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTACCGCAGGCAGAGGTTAAATTCTTTTTGTGAGCTACACCTGAAGTAAGAGCAAATAGAAGAGTTGCCCAAGATTTAGAATTGGGTTTGAAGGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTATAATGACACAAACAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCTGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGAACTGGCAAAAAATTTAGAGTAGTTGATACTGGCGGTATAACTATTGAAGATGAAGATTTTAAAAAGCAAATTAAACTTCAAGCACAAATAGCTATTGATGAAGCAAATGTAATTGTTTTTTTAATAGACGGCAAAGAACCGCTTACAGTTGAAGATTATTATGTTGCTAGTTTATTAAGAAAAAGCAATAAACATGTTTTGTTGGCAGTAAATAAACTTGAAGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCACTTGGATTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAACTTGTTGGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAATCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACCTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGTACAACGCGAGACTCTGTTTCGTCTCTAATAAAAATTAACGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATTGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGAGATTTAATTGAAAAAGATACAAACACAATGGAAAAATATAAAAAAGACTTGTTGAAAAAAGTTAAATTTGTCGATTGATCTCCTATTGTGTTTATATCAGCCAAAGATAATTTGAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAATAAAAACAAAAAAGAAGAGAGAGAATAACAATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCTGTGGCAACTATCTTAGCAAATAATGGTCATAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGAAAAGATTCAAATAAATCTTGCCACGAAATAATGACACAAGAAGCCCAAACCAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGACATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGTGGGGGTAAGCTGAAAACGATTTTTGGATTGTCGTGCCTGGGTGATTTAATGCTAACAGCATTGTCGGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCACTTTATCAAATAATATACAAGAATGATAACCCTAATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAACATAAGGAATAACAATGAACAAAAAAGAACTACTAAGTAAAACTAGCAATGAAACAGGATTTGCACATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGACGAAATAGAGGCAGGAAGAGAAGTTACAATTTCGGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCACAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTCAAATTCTCAACAGCTTTTAAAAAACGTATTAACAGCAAAAATTAATTTAAACGCCCTTTATATGGCGTTTTTATTTTAAAATGTTCAAAAAATCTAATATTCCAGGAAAAATAAGCTTTATTTCTTTACCTAATGATTTTATTGTCTCGAAAAAAATATATGATTTTTTTTCTTCAAATAGGCATTTTTTAAAATTAGCATGTTCAATCAATAAAAACTCGTTTTGAAATTTAAAATAAATTATTCAAAACGCAATTCCTTGATGCAATACAACTTTATTTAAATATTCAATTTGATGTTTTTTAATATTCTTTTTTTCAAAATTTTTTTGTTCAGTGCTTTTAACTTCAAATGCTAAAAATCTTCCTTTTCAAACTCCATAATAATCTACTGTGCTTTTTGAATAGATACTTGCATCGTTTAAAAGCAATTTATTTTTTTCTAATTTAACATTTTTAAATTTAATATCCAAATTTTTTTTATGAATTAAAGCAATTCCATTTTTAAAATAATACTCGTTAGTGTTATTTATTATAGATTCTAAAAGCATACCTCTATTATTTTTATTTTTCATATAAAAATTACAACATATTTATGCCTTGATTTTTTCGCTATTTTTTCGCTTTTAAGTCTTTATATGTTTCGAAGTTTTTAAAATAAATATTTTTTGATTTTAATAAATATTCAACTAAGTCAAATGCCATAATCATTGCCAAAACATCATTATGGCAATATCTTTTCAATTGAGCTTCTTTGACTGTCCATTCATTATCTCTTATTTTGTTTAAGGCCCTTAGTGTCGCTATTTGTAAGGCCATTCCACCATTTTTTACATCAAGTTCGTGATATGGAAAAATTTTATGTTTTAAATTTAATTTATTTTCATTTACAAAAAGCTCAATCTTTTTTATTGAATAGACATTTTTTATTTGCCCTAAATTTACAATAGAGCTTTTTATATTGCAATCCATTGCTGGTCTAAAAAATAAATTTTCTATATCAATAATCTTGCTTAATATAAATTGAGATTTTTCTTTTAAAATGTCAAATTCATTTTGGCTAATAAATCCATCATCTTTGTAATATTCCAAAATTTCTTGCATTTCTTTTATTCTAGATGCTTCATATGTGTCGTTATATACGACATAATAATCTGCTTTCTCATCATATAAATCATTTATTACTTTCATAATTGTTTTGTAGTTGTAATTTAAAGGGTCATAAACAAAATCATTTGAACTATATATTTCACCGTTTTGAGTTTTTATTATAGATGTTTGACTTATTAATTGATTTCATGCTAGAGAGTGGTTAATCATTGGAACGGGCAAGGTAACGCCTTCGAAATCAAACCAAACTATTTTTTTATTAAATTCTTTTAATAATAAATATTCAGGGCATTTATCCTTGTTTAAAATATCGGGTGAAATTGAAATTAAATTGTTTTCATAAAAATCTAAAATTTCTTTGGTTTCAATTGAATTATCAGTTAATGATTTAACTTTTAATTTTAATAATTCCTTTTGACAAATAATGTATTTAGGAATAACCTTTTTAACTAATAATTGGTAGAATTTGATTCCTAAATTTAAATTATCTTTTAAGTCATCCATTGTTATATTGCAATCTAGTTCGTGTATTTCTTTATTACTATCAAAGTTTTCAATAATTTTTAAAAAATCAGAAAAATTGCAAGTGTATTTACTTATCTTGCTTTTTGCATCGTTATCTTCAATTTCAAAACGACTATTAAAATTAAATGCATTATTTAAATTTAATTCATTTTTTACATAATCAATTATTTTGTCATTTTTTATTTTTTTATTATCTTCATTAAGCAGAGGATTTTTATCAAAAATAGCATTAATTTCAGTTTCTGATAAATTCTTTTTTTTATCAAATGTTGCTTTTGATTTTTTATAATTGCAATATTCAGTCTTTATTATTTTTAATTCATTTTTTTTCGAGTTTTTTCTTGTTTGATATCAAGGTTTCACAATGAAAATTTTATTGATTTCAATTCCTAAGGATGAAAGAACGGAATAATCATAATATGCTCTTAATAAATCTTTTAGGCTAGTTTTTGATGAATATACAATATTTCCCATTTCTTTAGTTTTACTATCGTAGTAAAAAGGCTTTGATGTGCATTTTTTATATTCAAAAACAGGATTTATTATTAGCGAATTTTGCATTAAAAATTCTTTTGTTTGTAAAATTTTTTCATCGGTTGAAGATTTTTTAGAAATTATATTTACTTTATTTGAAGAATTTTTTGTTAAAAAATTTATAATATGTTCCTCAACAAGGGCTATTCCACTATCAACCATTTGAAACTGAAAAAAATTATCCAATATTTTGTTAAATAATTCTAGTTTACCATCTGAATTAATTTTTTCAAACAGTGTTGAATCATTTTGCATATCATGTTGAATTTTTTTTATTTTTTCAATAGTGTTTAGCTTTAAAAAGTCTTTGTTTTTAGTAGAGTTATTTTCAAAGTATTGATTAAAATTTTTGTCAAGAATGTTTTCAATATCATCATTTGCAAATTCCAACATTTCATAACCGTTGTCTTCTTGATTGTCGCTTTCATAAACTTCACTAAAATCATTCAATAATTCTTCTTCTCAATTATTAAAAGCAAAGTAAGGTCTGGCAGTATTTAGAATCAAAAAATTTTTATAATTAAAGTATTTAGGCTTGTTCATATTCGGGAACAGCCTTTCCTTGATCTACTCAATATAAATATAAATTTTTCCATTCTTCTTGGTTATTACATTCAAGGAATTTTAATAAATCTTGGTCAATTTGTTCATTTCATTTTGGACTTATTCCAATTTGAGTGTATTTCTTATATCATTGCATACAATAATTAATATCGTCTTGGACTTGTTCGTTGTTTAAGATATATGTTCAATTCTTTATTATTCTTTTAGTTATGTCTACGTCGTTTGGATTTAAATAATCTTCTTCTTTTAAAAAACAAGCAACGATACTAAATGAAGTTGCTCCAATCAAGTATGTGTATAATTGAGCTTGCTTTATATAGCCAGGATTAACTCCATATTTTATTCAATTTTCATAATTTTTTTCCCCGACAGTTTTAATTTCAATAATTAAATGTCTTTCGGGACTATAGCCATCAGGCAACCCTCCAAATAGTTCGTTATCTTTAAAATAATCATAATTATATTGATTGGCTTCATATCTAAGCAATTTGGAATTTAATTTTTTTTCAATAATTTTTACTATTTTTGGTTCTAATGCAATACCTGCATCAACATATTTCCTGTTCAATATTGGTTTTCTAAAACCGCATATATTACAAAATGCTTCAAATTGGCTGTCAAATGAGCTCAATCCCATTATAGCTCCCAACGCCGAACCGGTCATTTTTTTAAATCCTCCAAAAGTACCAGGTTTTTTTTCTTGCATAGATTTTAAGATTTTTGGATCTACTATTAAAGTCTTATTTTTATAATCCAAAGTGTAATTTTTATTGTTAAAAAATGTTCTTTTAGGAATTATTATTTTTTTTTCCATATTATTGCCCTTCTTAATTAATTACTATTAATGTAATGAATATTTAAATTATATATTTTCAATAGTTAAAAACTAACAAAATGGGAAAAAATAGCGAAAAATTAGTTTTATCAAAAAAAATAAACAATTAATGTTTAATTTAAAGTAAATAAAAAATGTTTTTTTTATTAATGTTCTAACACTTAAAATTTAATTAGTATATAATTTTTTTTGTAAATAATGAAAAGAGGAATGATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTAGTAGCAGCAGGATGCAATGATCCAAAGAATAAGAAAAACCCAAACAATAATGATCAAAGTCAAGATAGCCAAATTGAGTTTCAAGAAGGCTTGGGGTTAAAAATTGCAAAAATCGCCAAAGATCAAGAAAATGTTAAAGTTGATGAATTTGTAAAAGAAGCAAAGGGTGCAAAAACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGAAGCAAAATATGAAGTTGCTGCTGGTACACACGGTCATGCCGATGAAGGACTAGTACACTTAGATATTATTCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAATGGAAACTGTTGAAGACACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGGCTTTAATTCTTTACTTGAAAAATTAAAAGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACGAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAATTAGTTTATAATTAAGGCAATAATGAAAAACAATATTTTAATTTTATTTAAGCACATTATTGTTTACTTTTTTGTCTTGTTTGTCATTGTTAGTATTATCTTTTTTGTTATTAACTCATTGATTGAACCACAGATAAAAAAGAATATTGCAGTTTATTCACAGATAAATTCAAGCCCATGAATTAGATATGGCGAATTTTTGAATAATTTGTTTTCATTTAGAGGTGGCAAGATTTATTCAAAAGAGCTTGCAGCAACTAATTTGTCAATAATTACATTGTATTTTATGCAATTTAAATGAACAATCTTATTTACTGTAATTATTTTTATTTTTTCTTTGATTATTGGAAATTTACTAGGAATTTGAAGTGCATATAAATTTAATAAAGCACCCGATTTTGTCGTAAATATAATTGTTAGTGCTTTAGCCACAATACCTTTAATAGTTCTTGCAATATTAGCATTAGGTACATCAATTTTTCTAGGATATCCAAGTCAATTTATAAATAACGGAAGTTTAACTTTTACTTCTATAATTACACCAATTATTATTACGGCATTTGGCACAATTAGTTTATTTCATGCAAGATCAAGAAAAATAACAAAAGAAACTCTTTCTTCTAATTATTATTTGTTTGGTATTTCACTAGGGAATTCAAAAACTAAATTATTTAAACAAAACATTATTAAAAATTTAATTATTGCTGAATTACAAGTCATTTTGCCTTTTTATATATTGCTTTTTAGTACTTCATTAATAATTGAGAGAATTTTTTCAATACCAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTGAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCTATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTCTTTAATAAAAAGAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTGCTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCCTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGTATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGCGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATTAATATTTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAATCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTCTAGCAGCATTTTATGTTTTTAATCGTTACAAATGATATGGAATTGTTATCGATAAAATTGCTGTTACACTTAATGCAATACCCGAAATAATTTGAATATTTTTGTTGTGTATATTTTTGGGAACAAATTGATATGGTATTTTAGTTGCTTTCATATTGATTAGTTGAATAAGTTTTTATGAACTTTCAAAAAATGAAATTCTTAATTTAATTCAAAAAGAATACATAAAAGCAGCTAAAATAATAGGTTTAAATAATTTTCAAATTACTTATAGACATTTATTTAAAAATGTCCTACCTTCTTTAATTATTTTAATTGCTGATCGTCTTGCAATAAATATTCTAATTGTTGCATCCCTTGCCTTTCTAGATTTCTTAACTGCTGAAAGTAATTTAAACATAGGAACAATTTTTAAAGAAGCATTATCTTCAATAAAAGTAAATCCTCAATACTTGATAATTGTTGCTGTTTATATTACTATATTCTGTATTTTACTTAAACTTTTTTCAATAGGTCTTTCAATGACATATGATCCTAAGATTTCTAAAAATTATTAATAGAATTTAATTAGTTTTATAAAATTATTGTTATATATCGCTAATTATTTAATAATATAACGAAGCTAGATTTCTATAAGGAGTTTGAATTAGTTATGTTGAATTTTATTTATAAAAACGATTGCAAAATCATATTTGGAAAAGATTCAATTCAAAATTTAGAAGAAGAATTAAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAGTAATTAAGGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGTAATGTTGTTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATCGGTGGCGGTTCGGTAATCGACACCGCCAAAGCAATAGCAGTTGGGGCAAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGCGCATTACCTATTGGAGTTATTTCGACAATTGCATCTAGCGGTTCCGAATCGTCAAATTGCTCGATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTTGCAATAATTGATCCTTGATATACAAAAAGCCTCCCTATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATTTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAATGCTAAATTATTAATGGAAGATCCAAATGATATGAATGCAAGGTCAGAAGTATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGAGCTTCGCATAGAATTGAACATGAATTAAGCAATTTTTATGGTATAACTCATGGCGAAGGTATGGCGATTGTCATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTAGACAAGGGTTTGAGCCAAGAAGAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGATAGTCAAACCATTGGTCATTATAAACCCCTTTTATCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAATAATTAATTTTTATATTTTATAAAAAAACATGCCTTTTTTTAAAGCATGTTTTATTTTTTTACTATTGAAGTTAAAACTTTTTCTACAATATACTCATCATTTTTTTCAAGGTTGCAAAATTTATTTAATGCAATGTTATATTTAGCAATTTTTTCATGAGATAATGCCTCATAAGATTCAATTAGTATTGAATTATTTCTATCGTAGTTCAGGCTTTCAATGCTTTCAATATTTGATATTGAAATAATAGAACTATTTAACCTTCTAGCAATAATCTCTGCTATTCTAAGATCAGATCCTGCTGCTATAATATTTGTTGTTTGACTAATTTTTCTAATGTAATATGGGCCATCTTCAAATTCAACTTTTGAAGTATCCTTTATTTCTTTAATTTCTTTATTTTCTAAATAAATTATTGATGACTTTTTTGTGTGATTAAAATGAAATTCAAATGCTCCTTTTAATTCATTGAAATCGCAAGGCCTCAATAAATTTATATGGTCATATGAATATAATATTTTAAGCTCATTGCTTAGGCTAAAATTATTTCTATTATTGATTAATAGTATAAGAATTTTTGCATTTGAATATTTTAAGACTTGCAATGTTGGCAATAAATAACCTAAATAATTAATATCTACAATGACAATTGTTTCTATGCTTGAATGCAAACCTAATCCCATTGCCATATTAATCATTGTAAATTCTCTATTACCTAAGAAAATTCCTCTTCCAAGATTGTTACTTGCAAAAATCCCATTTAACGATTTAATATTTTGAAAATAAGAAATATTATTTGATAATACTAGTAAATTTTTGTATTTATCACATAAATTATTTATTATTTTTAAAGCATTTTCAATGTATGTTTTTTCTTTAAATTCTTCAACATCGTTAATATTTTCACGAATATTGTTTTTTAAAAAGATTTGCAAATTATTTGAATTACTAAATGTTTTTATTTTGTTTTCTATTTTTTGCAATCTATTTTCATAAAACTCAACAACCTCAGAATACAAATCACTCAAATTTGTTTTTTGAAAAGAGGTTTTGTCTTTGAATTCTTCTAATTTTTCATGAGTTATTGAATTATCAAAGTTCTTTTTAAATAATTGTTCGCTTTGAGGACTTAAAATTTTAATAAACACAGGTTGATTAATTTTTTGTGCTTTTTTTATTGCTTTTATTATTGAACGACATTGACCATTTGATACTTCAATGTATTTGAAATTCATCGACTCAACTTTTTTTGCTATATTTTCTGGATTAATACTTTTACTATCTACTGTGTATTCAATTTCATTTGCATCATAGATAACAATTAATTGCCTTAAATTATGAGTTCCAGCATATGCTAGTGCTTCTTGAGTAGATCCTTCTTGAAGTTCACGTTCAGTTACATATCAATAAGTAAAGTGATTAATATTCTTATCTAACCTATTTAAATAATTTTGAGCGATTGTGTTTCCTAATGCAAAACCGCCTGATATTCCTAAAAATATTTCGCCGCTTTTAATTCAATCTAATGTTCAATTCGAATATGAAATGTTTTGATTTCTATTGCTTATTAAGTTAAGGTATTCATTTTCACTAATTAAACTATATAATCTCAAAATTTCATAAAAAAGTGGCAATATGTTGGGATTAAAAAAAACAAATCTATCCCTATTGACATCAGAATTATTTTTTCTATTTATATTGAAAAAATAGCCAAAAAGAGCATGAAATATTTTTGCCCCACTCATGATTGAAGAAAAATCACTATATTTTCAACTAAATGCCATATTTAAGGAATTGATTTTTATATTGTCAATAGCTATTTGATCAATTTTTTTATTTTTCATCTTCATAACCTTGGAATATTAAATTAATATTGTTTGGTTTGGCATTTATTAATGAAAAAACTTTATGTTCAATCTTAAATATTAGTTTTTTTGTTTCATATGCAAAACTAGTTTTGTTTATTAATTTGTAACTTAAATTAATAAAAACATTTGTTTTAGTGCGATTTGGTTTAATTTCTATTTTTCCAATTAATTTTATATTTTTATTTGAACTAAAAATATTGTTTAATTCTTCATATATTGCATCTTGTTCAACAAAATAACGAAAGTTCATTTTTGTATTAACTTCAATTGCTCACATATTTTACGCTCTTCCAGTGTATTTACCAGTTTCGGTTGAAATAGTAACTATTTCACCTTCTTTTATAAATAATGGTGTTTCAACAACATAGCCGGTTTCTAAAGTAACCTTTTTTTGTGCTGCTTGAGCTGTGTTGCCTTTAACTGCATCTGGTGCTTCGACAACTTTTAAATTTACATTGGCAGGTAATTCAATGTCTAAAACTTCATTTTGAAATTTTCTTACTATTACCTTTTGTCCTTCTTTAAGAAAGTTTAATTCTCAATTAAGATGTTCCGAAGGTATTTCAATTTGTTCAAAAGTTTCTTGATCCATTAAAACTACAACGTCTCCTGCATTGTATAGATAATCCATATTTGATTTTTCAATCATTGCTGGTTCAACTTTTTCGCCACCAGTAAATGACTTAATTGTTGTTGAACCTGTTCTTAGATCTTTAACTTTTGCTTTAACATTTGCTTGGCCGCGGCCTTGTTTTGAATGTGCTGCTTCAAGCACTACATAAATATTGCCTTCTAATTTAAATGTAACTCCTGGTTTTAAATCGTTAACATTTATCATAACGTACTCCTTTAAATTTAATTAATAAGGTATTTAATCTATAAACTTAATAAATATAAATTATAATTAATTTATGGAATTTTACATTTATTATTGTTAAATTTAATGATAATTGAAATAAGGCAGGTTAAAATGGAAGATTTATTAAGAAAAGTTCCTAAGACAAAAAAAGAATTGAAGATATATTCTATATTAATCAATAAAATAGTTTCTTGATTAAAGCAAAAAATAAACTCCTCAAATTCTAAAGGTATAACATTAGGAATATCTGGTGGAATTGATAGTTCAACATTAGCGTTAATTTCTGAATTAGCTTTTCCGAATGGTTGCAAATTTTATTATTTAAAAACAGAAAATGATTCATATACAGAAAGCCATATTAAACTTTTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAATTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAGACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATATGGTCTTGCATTCCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAGGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGGTTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAGGGTCCAAAATTAAAATAATGTTCAATATAAAAATTCAAGCATCCTTAACCTAAATGCTTGAATTTTTATATTGTTTTTTATGCTTTTAAATTGTTTGCTTTTGTTCAATTGTCAAAAAAATTATCTAAGTTTAATGTTTTTATTGTTGAAATGTTTAGTTTAATTAATCCTTTGTTTTCAATATTAAATAAGGGGTTGCAAATATTCAAGGTATAATCATTAATTTTTTCATTTCACAATTTAAAATTACTATTTAACTTTAAATTGATAATGCCATTAAAGTGACTTGGAATGTAAAATCCTTTTTTGATTGAATTATTATATGATTTAATTTTTAAAAAATCTTGTGTTTCTTTGCTAAAAATATATGAATCATTTAGAAAGTAATTAAAAGTATAAGAAAGGGGTCCATTTTTTTCTAAAGTATATTCTAATTTGTTAGTAATATTTAATTTATTATTTTGACTATAAAAATTAATTGAATCAAAAATTGGATTTTTTAATTCTGGATAATCATAAATAATTTTTTTGTTGTGTTGGTTTGTTTCAATAAAGCTTTGCGAATATCCAATGTGATTTCAGGGCATATTTTGTTTCAGCTGATTGAATTTAAAATATGTAACAAAAAATTCTTTAACTACAGTTGCAGAAGAATTATTAACATCTTCTTTAAAACTCTTGTCAGTAAGAAAAATATCAATATTTTTTAGGGTTCTTGTTTTAAAATCTGAGTCTATATTTCTATTATCTGATAGGTTATTTGCTAAGTCATAGTTAATAAGAATATTGGCTCCGACTTTATAGCTCAAATAATCTCTAATTTTTGTATAAGCATCAATTTTTATTATCAGTTTTTTTTCTAAAAATATAGGATTTCCATCTTCATATTTATCAATTTTAAAGTGAATATATTCATCATTTGTTTCATGATAAATCGACAAATTTAGAGATTTTTTATTTATTGAAATTTCTTTTTTTGTAGCAACTTCATAAATCAATCTATGAATTGAGAGAGTGATTGATAATTTATTGCTTTTTTCATCTCTATTTATTTTGATAAATATTGGATATTTTTTTAGCATCGTATTAGATACTAAAGTATTTGATTTTAAAGATTTTGAAGCAGATATTTTAAAAGTTACATCTTCACAAATATAGTTTTTTGCTAGGATATCATTTGCCTTTTTTTCAACAACTATTTGGTACAATCTTTCTTTTCTTCTTTAAAAATATTTTCTTTGAATTCACAAATCAATCTAGTTTTTGCTATAAAATACAAATAATAAAAGGGGATTCAATATTTATATAAATCGTGTTGCTTCTTTTCTTTGTCTATTTTATATTTTTCCATTGATCCTCCGGTGTCTAGAATTTGCCTTCTAGACATAATAATGTAACCATGAAATTTAAGGATTTTGCCAAATTTTGGGAATTGTGAAATCTCTTATTTATTACATAAATAAAGAAAATTTATTGTCTAAAAAATCAAGAATCAAGCAATTGCTAAAATTAAATAATTTAATTTACGATAATATGATGATTTAGAAAAGAAAAAGTCTGTTCACTTAATATCATCTTTAAGTTCTTTAAAATATTTAATATAAATACATTTCAAATCATCACTCAATGAAGATTCAAACAATTTTGCTGTTTTTAAAACATATTTTTGAATTAAGAATTTAAGTGAAATTAAATTCTCTTTATTAAAAATTTCGGGAATTTTATTTATATCTATTTTAATTTTTCGTTCCCTTAACTCTTTTATTATTTCCTTAAAATAATTAAAACATTTTAAAAAGAATGCTCTGTTGCTACTACGACGCATTTTTTGCCTCCATTTAAATTGCGTTTTGTATGATTTTATATCTTTATTTTTAGTAAATAATTTCAAAAAAAGTTAAATAAAAATCTAAAAAAAAGTAAGTTATACAAAAAAAACTTACTTTTAGGTTTTGTATTTTTGATTTTATAAATATTTGTCAGGATGTTCAATTTTCATTTTTTTAATAAATTCTTCTTTATCAAGAGAACCATATTTTTTCATTAATTCTTGACATTCTTTATATTCATTTTCAGCTCAAACAACATCTTCAAATCCTGTTACTAATGTAGCATTTTTACCTTTAAATTCTTTATAAGTAGAAAAGAAATTTCTTACTTGAACTAATCACATTTGATCGACATCTGCCAATGATTTAATATTATCTAATCTATAATCATCTGCGTGGACTGCAATTAATTTAGTATCTGTTTCGCCACCATCAATCATCTTCATTGCACCAATTAGTCTTGCATTTAATGATGTTCCGGGTAAAAAACTTTCTGAAGAATAAACTAATACATCTAATTCATCTCCGTCTCAATCTAGTGCATCAGATAAATAGCCATAATTTGCAGGATATTTAAAATCACCTCTTAAAATTCTGTCAACTTTGATTTTGTTGCTTTTGCGGTCATATTCATATTTGATTTTTGAATCTTTACTAATTTCAATGTTTACTTCTAAATTTTTCATATGCTTATTTTATCACTTTCATAGAAATTCCTTATTGTTTGGGAAAAAAATGAGAGTTTTCGCTTATTTTTCCCAAATGAAATAATAGTCAGAAAAATCCAATAAACTTAAAAGTATGAGGAGTAAAAATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACTAACTATTTAATTTTAGATCATAATGGTGAGGGCGAATTGATATACGTGGCGGATATAAAAAGATTTAATAAGGATGATTTGAGAAAAATATTTATTTACGATGTGGTAAATGAATATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCTAAGACAGCAATTTCTCTTCTAAATTTAGGATGAGAAAATTTAATTCAACTAATTGCAAACGAAGATAAAGATAAATTATGTGAAGCATCTTATGTAAGTTTAAGGATTGCAAATAATATAATTTTTGCCTATAAACAAAAATATGCAAAATTTTTATCAAAATTAAGCGATGAAGATTTACTTAAAATCAAGAGCAAAAACACAAGCGACTCTTTAAGAGAAAAATTTGAATATACAATGAGAATGCTCGGTTTTAAAAAACAACAAATTTCATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAACAAATTGATCATTTACTATTTTACGGCCCGCCAGGTTTAGGAAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGCAATATTGTTTATGTTCAAGGTCCATTAATCGAAAAAAAGAGTGATGTATTGACTATTTTTTCTTCAATAAATGATAATGACATAATTTTTATTGATGAAATACATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAGGGTACAATTGACGTTGCCTTAGGGGTTGATGGTGATAAAAAAATCATGAGAATGAAATTAAAAAAATTTTGTTTGATTGCGGCCACGACAAAAATCAATTTGTTATCCAAACCCTTAAAAGATCGTTTTGGTTATATAGGAAAATTAAGCGATTATAGCGATTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATAAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTAGAAACTGTTAAGAAAGCCTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTACATAAAGTATTTAATGACAAATTTTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAATTACTAAGTAATTTTAATATTTTAGATAACATGCATTTTTTAGTATAATTATTAACTATATGAAGAAAACAAAAATTTTAAACAATAAATTTAGATGAGTTATTAGCATTTTTTTAATTTTAATTTTATTGATTGTGACAATTTTAGGATCGATTTTTTATTTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAAACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGAGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGCGGTAATCCATTATTTTATAAAGGTGAATATGTTTCTAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTAAAAAATGGACCTCAAAACTACAATATTAGTGTAGAAAAAAATCCAGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACAGGCTTGCAACAAGTTGATCTTTACAACGTAATAGAAAGGCTTCACGTGAAGAAATTATAAATAACCCAGGCAATAAAATTTACTTTTGAATGAATTTGGCGGAATTCGTTCATAGAGCAAAAACTGAGTTTCCAGATGAATGAAAAGCAGCGGGCGAAAATCCTATAAATTTTGCTTATGTTGGAAATTCTGCTTTACCAGAAATAAAAAAGGACAAAAAAGGGAATATCATTGATATTAAATATCCTGTTTTAAAATCAAAAGAAATTAATGCAAGAAAATATTTGATATCAGCAGTTAACCCTGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCATCATTTTATTTGGCAAACGAAAATCTTGAAGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACTTACAATTATTTTTTGACTGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTTTATTTTCATTGTTTGCAGTATTTTTGATTGCAAAATATCGTTTATATGGAATCGTATCATCAATATGCTTGGCATTTATGATATTTGTGTTTTTATCAATTATTACTTCATTTAATATATATATAACGGGAGCGGTAGCATTTACTTTCTTAGTTTCTACATTCTTAACATTTTTAATGATTTGACAAATATTAAGACGTTTAGATAAAGAAGTAAATTCGGGTTCTAACGGAATAAAAGCAACAAATAAATCATTAAGGTTTGGAATAATATCAACACTTGATATTGCCTTTGCATTAATTGTGGTTTCAATATTTACTATTTTTATAAAAACTTCATATTTAAATGCGATGGGCATAATGCTATTTATTGGATTGGTTTCTGGATTGATTATTGGAGTATTGGTCAATGCGTTAATAATGTGAAATTTAATAAAAACAGAAACGTTTGATAAAAGAACAAATTGAATTGTTTGAAATAGCAAAAGAGCAAATTCTTTGATAGCAAAGATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTATGGAATATATAGCGGTGTATTTGGAAGTACAAAATTAGGAATTAATGCTAGTGAAACATTAGCAAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGTGATTGAAATAAAACTGAAGTTGATCAAATTATTAAATATTGAAAAGAATTAAATATTTCAAATGCAAATATTTTCTTTGAAAAATCAAGTTTAACTAACGACTCATATAATTTATTAATTTCTTCAGCGGACAAGATTGATTCAACTAAATTAGAAGCTTTAAAGACATTTATTAATACTAAAATTTCAGCTATAGGTAATGAATTTGATTTGAACATACACGAAATATCATTAAATGCAAATGGTGAATTAATCAATTTTGGATTTACTATTTTAATAATTGTGGTTTCATTAATAATTTGTGGAATATATAGCATTATAAGATTTGGTTTAATAGCGTTTTTTGTAATGTGAATAAACGAAATATTAGCAGTTATAAGTTCAATAGTTTGATTAATAATTTGCCATGGACAATTTAACAATGGAATTTTAGGAGCATTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAAATGACCAAATAAACAACATATTTAAAAAATATGTAAAGGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCTTCTACATATACATTGATGACGGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTACCAAACATTTGAAGATTGATGTATATAAAGAGATATTCAATTAAGCAAAAGAGAATTGAAACTAAATATTGAGAAACAGAAAAGATTAGCGAACAAACATTTGTTGGTATTAATGATTTTAATATCTAAAAAAGGAGGAACCAATGTTTCAAAGACTTAAAGGCACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTGATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAAGATAATGGCCAAAGAGACATTGCCTTGAGGCCAGAAGGAACAGCCTCAACCATAAGAGCTTTTGTGGAAAACAAAATAAACAATTTGGAGCCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGTCCTCAAAAAGGTCGCTATAGACAATTTATTCAAGGAGGAGTTGAATTAATTGAAAATTCAACTGTTGAGAATAATTTTGAAATAATAAAATTAGCATATGATTTTTTAAAGTCATTAAAAATTAGTAACATTACTTTAGAAATAAACAATCTGGGCTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAGGAATATTTTTCTAAATACAAGGAACAATTGAGCGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTGAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCTCCAAGATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAACAATTTACTAGATTTACTAGATGAATTTGAAATCAAATATGTAATAAATCCTTACTTAGTTAGGGGGCTTGATTACTACAATGATGTTGTTTTTGAATTTGTTTCAAATTCAGACGCTCTAGGTTCACAATGTACTGTTCTTGGCGGTGGAAGATATGATGGCATGATTAAAAGTTTTGGCGGACCTGATATAGGTTCTATTGGGTTTGCTTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAGTTTTAATGAAACGATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAGGAATTTTCAAGTAATTTTTCCAAGTCAAAAACAATCAATTAAGAGTTTGTTTAAACTTCAATATAAATTGCAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAATGAGTTGAAAATAAAATCACTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAAAAATTATTGTGGAACATTATGTATTAATGATTTAAACAAAGAAGTAGAGCTTTGTGGATGAATTGCAAACAAAAGAAAATTTAAGCAACAAGTTTTTATTGACTTAAGAGATTCAAGTGGCGTAGTGCAATTAATATTTCAAAATGTATCAGATCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAACAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTCGATAAACAAGGTTATATTGAAGTGGAAACCCCAATTTTAGGTAAATCAACTCCCGAAGGAGCTAGAGATTACTTGGTTCCTACTAGAAGAAAAGGAAGATTTTTTGCATTACCTCAATCACCACAACTATTTAAGCAATTATTGATGGCTGCTGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTTAGAGATGAAGATTTAAGAAAAGATAGACAACCAGAATTCACTCAATTAGATATTGAAATGTCATTTGGAAGTCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGAAAGGATGTACTTTCACAATTGGGGTACAAAATTGAAACTCCATTTCCAAAAATGGACTTTTTCTATTCAATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATTGAAGATTATTCAAAACAATTTGCGGCCAAATATAATAAGAAATTTGTAAAAGCTATTGTTTTTGACAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTAGACATATTCGACTTAAAAGAAGCAAACCAAAATGAATTCCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGAGCAATAATTTCAGCTAATGATTTAGAAGAAGATGCATTGAAATCACTTGGAGCAATTAGAACAATTGCAAATGATTTATACAAATTAGCAGATCCAAATCAATTGAATTTCTTATGATTGGTAAATTGACCTATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATACGATTTAGTGCTAAATGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTTGGATTCTTTTTAAAAGCATTTGAATATGGTCTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATCAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGTATTGACTTGTTGACATCAGCTCCTAGCGATGTGACAAAAGAACAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAATAATATATAAATTAAAATATATGGGTAATAGTTAAATTAATAATTTGATTTTAATTATAAACAAAACAACATAAAAAAAAGAAAGGGATTTTATGCATGCTGCTTCAATAGCCTTAACAGTTATATTGGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGCGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATTAAAAAAGTATTAAAGTGATCAATGATTACTTTAGGTATATTACTTTTTATATTCTTGATCGCTTTAAGAGTTGTAATACAAAATGGTTAGAATTTTTAAATAAAAATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCTAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTTTTTAGTGCCTTTCTTGCACCTTTTCAATTAAAAGCGGTATTAGACAAGGATTTATTGCTTGCAAATATTTATTATTATTATGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGCAAGAAAAACTATTGTTGGAACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGTGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAAAATAATTGAAAGCTATTTATTCATATTGCCGATGTTTCATACTATGTTAAGGAAAATGATTTAATAGATAATGAGGCTTTATTACGTGGCACAAGCATTTACTTACCCGATAGGGTTATACCAATGTTGCCGGTTGAATTGTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAACAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTAAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAACGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATTGGACATTTCGGTTTAGCTTCAAAAGCTTACTCGCATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTGATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCTGATTTATCAGAAACGCTAAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGTGAATCTGAAAAAAATGCAATGGTTGTTGAACGAAGTGTTGTTGATGTTAGAAAATCGGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAATATTGATGAATACCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTTGCGATTCCAACTAATAAAGACAACGATTTTCAAGCATCATCTAAAAATAAAATCTATAAAGCAGGCAATAAGTATTTAATTACTATCACTTCGATAGATCACGAAAAAGGAAATGTAAATGCAACATTAGCTTAGCATTGACATTTTTTTATTAATTAATTAAAAATAATTTTAATAGTTATATTTTTTTATAAAATAATAGTATGCCCAAATTGATAATTTCAAATAAATTTATAAAATCAAATTATGAAATTGATTCCACATATGAATGTGGCATTTCGTTATTAGGTTGAGAAGTCAAGAGTTTAAGGGCAAAAAATGCAAAACTTGATAATTCTTTTTGTTCAATTAGTAATAACCTAGAATTATGAATCAATAATTTAAATATTGCTCAATATATGCAGGTAAAAGGCGATTTAAAAAGAAGTAGAAAATTGCTAATGCATAAATCAGAAATATTAAAATTAAAAAACAAGCAAGAAAAATTTTCTTTTGTTATTGTTCCAACTTCGATATATTGAAGCAATAATCATATTAAGGTAGAAATAGCTTTAGCTAAAGCCTTAAAAAAATATGATAAGCGAGCTAAAGAAAGAGAAAACGAAATAAAGAAACAAATAAAATCAAGTTATTAAGGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAAACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCTAATGAATAGCTCGGTTCATTGCTAGCCTTTGGAGAGCTCAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGATTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAATGAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCATATGAAAAATAAAGAGATCAGCGTTTTATGATCTCTTTTTCTTTTAATTTGGTGATATTTTTTTTAAAATAAAAATAAATAATAAAAATTATTTTTATTTTTTATAAAATTAAAATAATCTAGTTATTAAAATTAATAAATTAAATATAGTTTTATAAAACTTATTTAACTTTAATATAATTTTAACTATAAATATATAAGGAGGGGAAATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGAGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAAAAGTTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATCGCTGAAGCAATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTACTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGCAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCACAAAAGACCCAATCACATACGACCAAGTAAAACCTGGGGCGGTTATTATCGATGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAATGAATTTTCTAATGTTGCCTCATTTATAACCCCTGTTCCCGGAGGGGTTGGTCCAATGACTATTTCGGCATTGCTCATTAATCTTTTAAAAGCAAGTTCCTTGCAAAATCCAATTATTGTACCATATCTTAAATTTTTAAAATCATATTTTAATTAAAAATTTTTTAGCTGATTTTGAAGGCTAAAAAATTTTTTTAAATTTGATTTATATTATTATTTTTTTGTAAAATAATATCACATAAAACAGGAGGGAAAAATGATTATAGGTGTTAGTGGTATGATTGCTGCCGGTAAAAGTTCATTAGCTGAAAAACTTCATCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGACGACGAAGTATTTAATACATTTTTGAAATGACTTTATGAAGGTAAACCTAATTTAACAATAGGATTTCAATCATATATAGTCGAAAATCATTCTACAAAATTTAATAAAATTAAAGAAGAGTTTGAAAAAAGCAAAAAAGATCCAATTGAAGATATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTAATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATATTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGGCAAAGTGAAGTAGATAACTGAGATGAAAACTATGAATACTTCCGTAATTTACATGAAAACTATTTTAAAATTTTTAAAGAAATTGCTCAAAAATTTGGCTTAAAATATGTTGTAATTGACACAAATAACTTAACAGAAAAAGAAGTTTTTAACAAAGCTTTGGACATTATTGAGAGTGAAAAAGAAAAATGTCGCAAATAGACTATAGAATAAAAAAAGTTCTTTTTGATCAAGCAACATTGGAAAAGCGCATTAAAGAATTAGCCAATTGAGTCAATGAAACATATAAAGATTCAAAAAATTTAATCATAGTTGGTTTATTAAAAGGCTGTTTGCCATTTTTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTGGATTGCATGATAACTAGCTCATATGATGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGATTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCGCTGCTAAAATCAAGAAATCCTAAATCCCTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTGGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAAACGACTTTGTTGTTGGCTTTGGCTTCGATTGAGAAGAAAAAATGCGTCAATTACCATACATTGGTTTATTGACAGATAAAGAAATTAATAAACACAAAAAACACTAATAATTTTTCAATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGCATGCCTTTAGCTATTGGCTCTGCGTTTGTGGCATTTATTTTAATAGTTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAATCAAAAACAAGAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAATTAAATTCCTTAAAAATTGAAAAACAAATCGCTTACTTATTTGACTCGCAAAAATTTTTTAAAGAAAATTTAAATAATTTATGAGTTAAAAAATATTACGAAATTATTAAAAGATTTAAATTAGAAAAATCGCCTACAAGTAAATTTAATAAATTAAAAATCAATAAATATTACAAAAATTATCTTAATGAAGTGGGCCAATGAAATTATTGCAGACACCACATTGATGAAATAAAAATAAGTGGAGCGATTTATAGTGCTCATCCTCAAACAAAAGAATATTATAAAACTGGTGATGTTATATTGGTAACATATGAACAACACTTATTTTTACATTATTTAATTGTAATGGCAAAAACTACAATGCCAAACCACGGAATGATAGTGGGACTTAAATTTCATTTTAATGATGCTAAGAAATGTATTGAATATTGAGATAAAGTTATTCAAAAAATGTGCGAAAAATATTCAGTAAAATATGACAAAAATTGAAGTGAAAATTTAACTTAATTTAAATAGTGAGTATTCACTATTTTTTATTTTAAAAAAACTAATTTTCTAGATTTATTATAATTATTAATATAAAATATCAAAAAATATTAATTGCAATTTTAATTAATAAGAATTTTTAATTTATCAATAATTTTTAAGGAAAAATATGAAAATAAAGCTTTTTAATAAAATAATAAATGATGAAGATAAACCTAAAAAAACTCATTTAAGTTGAACAGAAAAATTTAAACTTTCGTTGCAAAAAGAAAAAGACAATTTTAAAAATTCAATTCAAAAAACAAAAGACTTTTTTTGCAATCCTAAAAAGTTTTTTAAAGATTATAAAGAAAATGTTTTGAAACCAGAATGAAAAGGATTTGTAGAAGGTTTTAAATTTGAAAATTGGCCAATTGTTCAAGCAATAAAAAGACTAAAAAATCCTAAAAATAACATTAAAATTAAAGAAGAAAAAATTAAAATAGCAAGATGAAATTTTACTGAGAATAAAGACGAAGATCAAACAAATGATAAATAAAAATCTGCTAAAATTTTAAATTTTTTAGGTTTTAAAAAATATTTTATTACCTAGGCTTTTTATAGCTAATTACCATATTTATAAATGTTGCATAAAATATTAAAAAAATTATTTAAAATTAATTTTTTAATAGTGATAAAATCACTATTTTTTATATTTTAAATAAAATATATTAACCTTAATTTATTATAATTATTAACATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGTTCATCGAAGCTGGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGTCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACAGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAGGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTCGACGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATAAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGTGGTCATGTTATTGCTCAAGGCAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAACCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCCATTATTGGCCCAACTGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTACAACTGGAAAAATTATCTTTAATGAGTTGCCAATAAAAATTAAAACTCCAAAACCTAAAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGAAAAAAAGATCTAAAAGATTGAAAATTAAAGAAAAAATCCGAAAGGTTTGAGTGAGTTACAACCGATTTAGAAATTTCTAAAACCATAAGAAAAATAAAAAATATTAAATCATTAAGAAAACAAGTGGGAGTTGTATTTCAATTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGATGAGGCTAAAAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGATTATTTGCAAAGAAGTCCATTTAATTTATCAGGCGGTCAAAAACGTAGAGTTGCGTTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAACCGACTGCCGGGCTTGATCCTCAAGGTGTCGAAGAAATGTTAAACTTATTTCACAATCTCTATTTAAATGGAAAAACGATCATTATTGTAACGCATGACCTTGATAATGCTCTAAAATGAACAAAAAGAACAATATTTGTAAAAGATGGAAAAATAATTAGAGACGGAAATACATATGATATTTTGTCAGACGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCATTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAAGATTTAGCTGAAAAAATTAATTATTACTTAACCATGAAGCGCAAAGGAGGAACAAATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACGTTTATTCACAAAATTGATCCTCGTTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGAGTAGCCACAAAATCATTAAAATCACTATTTTCTAAGTTGCGCTTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGAGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCACTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGAGCCCCATTTGGAAGTGAAAATATATTTCAATTAACCAAATTTACAATTTATAGAACGTTATCAATATTTTTAAGAATATATGGCGTTATTTTAACAACAACAATTTTGACAGTTTCAACAAAACCAGTCTTACTAACCAGAGCAATTAATGATATTTTAATCCCTTTAAAGTTAGTTAAAATTCCTACAGAAATTATAACAATGATTATTTCAATATCATTACGTTTTATCCCAACATTGCTTGAAGAATCCAGTCGTATCATGAAAGCACAAGCAAGTAGAGGTATTGATTTTAAAAATGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCTAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAGAGATCTCACTATAGAGTGTTGACTCCTTGTTGAAGAGATATAATTGCTGTATTAGTCATAGCTAGTTTAACAACGTTAGTTTTAGTAGCTCAACTTGACGCTGTTCCGTTGCCAAAATGATGATTAGTAACATTTCAAAGAGTTTAATATGAATAGCAATAACGATATAGAAAAAATAAGAAAAGAAATTTTTGATTTGCAGGCTAAAATTAGTGCTTGAGATAATGCATATTATAATTTAGATTCGCCTGAAGTTCCAGATGAAATTTATGATGTTGAATTTAATAAATTAAAGGCACTTGAAGAAAAATATGCCTCGTTTTTTAGTAATGAAGAATTAAAAAATTCTCCAACAAAAAAAATTAATGCATTGGCTTCCGATCTATTTAAAAAAGTAAAACATAAACGTCCGATGCTTTCATTAAATAAAGCATATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACGTCTGATTTTTCTTTTTTTATTGAGCCTAAAATTGATGGCCTTTCAATTTCTATTATTTACGAAGATGGCAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTATTCAAATGTCTTGCATTCCTAAAACTATTAAATATAAGAAATCATTAGAGGTAAGAGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGGCAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCAGCAGCAGGCACATTAAGGCAATTAAATTCTCAAATTATTAAAGAACGCAATTTGTCAGCATTTTTGTATTATGTTGTAGATCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAATTCTTAAAAACTTATGGGTTTCCCGTTACTCCCGAAGTTCAACATGCTAATACATTGCAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAATTATTAATAGATTATGAAACAGATGGAGTTGTTATAAAGTTAAACGAGGTTCAATATTTTGACAAGTTGGGATCAACAGCTAAGTTCCCCCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATATGCGACAGTAGGAAGAACTGGATTGGTTACATACAATGCAGAATTAGAACCTGTAGAAATTTCGGGTAGTCTTGTAAGCTTTGCAACGTTGAATAATTTCCAATACATAAAAGATTTGAATTTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAACAATTTTGCTCAAATGATCAATGCCCTGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAGGATGGTTTAGATATTAATACTATGGGTGAGAGAAATATTGATCTATTTTTAAAAAATTCTTTAATTTCCAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAGTCTATTGAGAATATCTTAATTTCTATTGATGAATGTAAGAATAAACCTTTATATAAAATGATTTCTGCTCTTTCAATCCCACTAATTGGTGTAAAATTAGCTAAATTCGTAGCCTCAAAAATTAAATCGTTACATAATTTATTAAATTATGATTTTTCACAATTAATTGATTATCATGAAATAGGAGATAAAATTATAAATTCTATAATTTCTTGAATATCTAATGATTTTAATAAAAAGTTAGTTGAGGACATTATTAATCTTGGATATGATTTAAAAGATGAAAATGAATCAATTACTAATTTATTGGAAAATAAATCATTTGTAATAACAGGAACATTAGGACATCCTAGGTCATATTTTGAAAGGTTAATTGAAAAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAATGCCGGATCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAAATAGTATTCTCTCTTTATTTTAATATATGTATATATTGAAATAATATAAAAATTAATAGAATATCCTAATATTGAATTTTTTTCTACTTCAATAATCAAACAACCATAGCTTAAAGTATTTATTACTATTTCAAATATTGAAACATACATTTTTATTATAGGTTTAACTATAAAAGCAAATAAACAAAATACATAGTTAAATATTGAAATTGGTAGAAAGAATAATTGATAAAATATTCCTAAAATGTTAAATTTTGGATTAAATGAAATTACTATTGAAATAGAAGAAATATAAGGTAATATAATAAATAGAAAATTTACAAATATATTGTTTTTGTAAATTTTTTTAATGTCTAAAATTAAAAAAATAATCAATGTTATTGTAAAAGATAAAATAAAAGAATAAGAATACATGGTTCTAATATCAAAGAATATCAATAATAGTGCTGCAAAACTCAATATGTCAATGTTATTAAATTTGCCTTTAAAAATGTTTTTGCTTGCAATTTTTAATATCAAAAACAAAAACGCCCGACTTGCAGATATTGAAAAATTTAATAACTTTAGGTAAAAAAATAACATTAATATTGAAATCAAATCAACAACCAATTCCTTTGAATTCAATCATTTTAAAATTTTTGATATAAGCAAATATATTATTCCAAAATGAAATCCGCTTATTGTAAAATAGTGCATAACACTTAGTTGTTTTAATTGATCTACAAACAAATCATTAGCTTGTTGAATGTTGAATAAAACCAGTGCTAGAAATTTTTTTGTTTTATAATCATAATCTTGCAATATTGGAATTTTTTCTTGATATGCATATGCTTTTGAAACCAATTTAACATTTAACAATTCAAAATCGATTTTATTGCTCAAACTAAACATATTTTGATTTTTAATTATTGATAAAGTGCCTGACAATTCGATATTATATTTGTAAAAATTATTGAATTCTCAACTGCTTCAATTTTTAACTAAGACATTATAATATTGATATTTAATTAATATTGATTTATTAAAAAATCTCTTTATTTCAAACTTACCACAAATATTCTTTCCATTTATTTTAAATAAATCCAAATATCACAAATAATGAACTAAAAATATTAAAGGAAATACTATTGCAATTTTTATTCAATATTTTAAATTAAAAAAATTCACTGCAATTATTAATGACAACATTATTGATGATATAACAATATTGATTTTATAAATAATTAAACAATATAAGCAAAAGCTCAGCGCTACAAATAAATTTGTATTTAAATTATTAAATGATTTTTTAATATAGTTAAAGTTTTTTCACCTACTCCTGGTAAATTTTCCAATTCCTGTCATGTTATTAAATAATCCTTGGCAATGAAGTAGCTTAAAATAATTTTTGCTATATCTTTTTTTATTCCTAAATCCTCTAATATGCCAACGTTATTGACATCTCTAATAGAAATTGAATTATCTTTTTTAAAAGGAATATTAATTGATTGATTTTCTTCTAATTTTAAATTTAAATCAATATTGTTTAAATCTGCGCCCGCTTTAAGATGTGCTTGTTTTATTATTTCCAATAATTTAGTGCCTTTTTTAATTTCGTATGAGTCGGGATATTCTATTGCACCCGATATTCCTATTGTTATTATTTCATTTTTGTTTTTGATAAATAATTTATTGTTATTTTTTATATTTGAAATGTATTTATTAATTCCTATTGACATTAGTATTATTATTGATGCTGATGAAAATAATCCTAGTCCCAAAAAAATTTTTCGTTTATTCATTCATCCCTCCATCTTTATCTTAAATTAAAGAATTTTATTATTGATGAAAATGGGAAAAATTAGCGAAAAAACCGTTTTTGATTTTATATAAAAAAATAAAAAACGCAAAGCGTTTTTTATTATTCTCAGATTGCGTTGTGTCAAACTTCTTGGACGTCTTCATCGTCTTCTAAAAAGTCAACAAATTGTTCAATTTGGCTTGCTTTTTCTTCATCTACTTCAATTTCGGTATTTGGGATATATGTAACTTCAGTTGTTGTATAATTTTCAATTTTGAATGTTTCATCTAATGCTTGTTTTACTTTTTGAAATGCTGAAGGGCTTGTAATTATTGTATAAGTTCCATCTTCATTTCTAAAATCTTCTGCTCCCGCATCTAAAACTGTCATCATGGTTTCTTCTTCACCAGAAACATTACTTGGAAATTCAATTAAACCTTTTTGATCAAAAACATATGGTATTATTCCTTGTTTTCCTAATTGACCATTGTGTTTTGAAAAGAAATGTTTGATATTCGAGCTTAAACGATTGAAATTATCTGTTAAACAACTTACAATAAAATTTACACCGCCAAATGCTGTACCTGAATATAAATAAGATTGATAGTTTGCAGCTTCTTTAGATCCGCCAGCTACTTTTGCAATTGCCTTTTCAATGTTTGCTTTAGGCATACTTTTTGCCTTAGCCTTAGCAATTGCTAATTTTAGCGATGGGTTAGAGTCTGGATCTGGTCCGCCTAATGTTGCAGCAACAATAATTTCTTTTGAAAATTTTTGAAACATTTGGCTTCTTTTAGCATCGACTGCAGCCTTGTGATTTTTTGTGGTAGCTCACTTTGAGTGTCCTGACATATTTTCTCCTAAATTTGATAGATAAATATAGGATATTATACCAAAAATAACACTCTTATTTTAGTTATTTTATATAATAAGAATATGTATTTAATAAGAGGAGAAGATAGTTATTTAATAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGATGATTATTCAGTAATTACTTACAATGAATCTATTGATTTAGAACAACTATCAGATGATATATTTAACCAATCATTATTTTCTTTTAAAAAACAAGTCATAGTTTTAAAAAACATTGATTTTTTTAATTCTAAGAAAAAAAATGATAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGTCAAGATAACAATTTAATATTTATTTTGCAGGATATTAAAAAAAATGATATTTCTTTTAATCCTTCATTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAATATATCTTCAACTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATCATTAATGAAGTAGATAAATTGCTATTGCAAAATAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAACAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAGATTTATTGCACGTATTTGTTGATGGAAAATACTAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAGAACAAACAAGCATAAATTAAAAGAATTAATAGGGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAAAGTTGGTGTTATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCATTGCAATTGATGATATATTATTGAAATGCTATGAAATCAATAGAAATTATGAATTTAATGATTGATCGAATTTGCAAGATTTTAAAAAATTAGTTGAACTTTGCTTGGCAAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAGCATTTAATTTCTATTTATCGAAACAAAAGCAGTGAAAATGAATCGAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTCTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTCTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGCATTAAGGGATTTATTCTTGAAGATTTTGAATTTTTAATTGACAAAAAAACAAATAACATAAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTAGTTCTATGGTTATTTGAAAAACAAAGCAATATAATGATTTTTTAAACATAATTGATATTGCCAATCAAAACGATTTAATATTTTTTGATTATATTTATTTAACTCATTTATCAGACATAAATTTAAAAAATTCATGAGATTGAATTAAATTCAATAAACTAAATGTAAAAAAATTATTTGAATTGTGAAAACCTTTTTGCAATAATGAACAATATATACTTTCTTTATCATCAAATTATAGCGGCTGGGTTGTAAACAAATACGGAGATAATTTATCATTTTATAAAGAAAGTGCGAAATCTCTATTAATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAAGAATATTTTTAATTTCAATAACATTAATTTTAATGAAGAAAAAAGATTTTATCAATCCAAAAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCGTGGACTAATAACATTCAAAATTTAGATTTTATACAGCCAAAATTGCGTAACAAAATTAATGTTGAACAACAGTTAACCAGTAAAGATAGCACATTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCTAAATATTCAATTTATTTTAAGGGTTTTCCCAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGTGAAAATAGAAAACTTTTGATTTTATTAAACTTGAATTCATTTCCAATAAAATCATACATATGAAATCAATATCTAATTCTTTCAAGTTCTTATACAAATAAATACTATTCAAGTATTCCGGAATATTTAAGTCCATATGAATGTTTAATTTTAATTAAAGATAATAATTAATTAGTTTATAATTTTTAATATATTTTATTTATTTAATAATAAAGGAGTCTTATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCAGCCGTCTTAGCTTTTTTAATTTGAAAATTAATTAAAAATAAAGTTTTAAAGAAGAGAGCTCAAAAAGTTGAAATACAAAAACAAAAAGAAACATTAGCATTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACATTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGAGATAAAAAAATACCTGAAACAATTAATTATTTTAAAGAACAATATGAATTAGTACCTTTAGGAAAAACAAGAGATGACTATATACAATTAGTTCAAAAATCAATATCTGAAAGATTTTATAATGAAAAATAAAAGTCAAAAGAAACAAAAGATAATAGTCTCTAAATTTGGTGTTGCAAATTGATTAACAGTTTTAAGAATATTGCTAATGATTCCTTTTATTGTTTTTCTTTCAACTTCATTTGGTTTAATTTTAAATGGTGTTCAACTTTCTTACAGAACCAACAAAGGAATAGTATTATCCATTTTATATTGGATAAATGTTGCAATTTTTATCATTGCAATGATAACTGATTTTTTAGATGGATTTTATGCAAGGAAAACAAAGACCGTATCTTCATTTGGAAAAATATTTGATCCAATTGCAGATAAGGTTGCAACTACATTGATGTTATTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCCGTAAAGAAAAACATTGAAGTATCAGCAAATTGATTTGGCAAAATTAAAACTATTTTAGTTTCATTAGCGTTAGTAGTGATTTCAATAGGAGTTCCTTTTATATCAGATAAATCAGATTATAAATATATACTATTTTATTTCAATATACCTTTAATAATAGGACTATTATTAGCGTGAATTAGTGGCATTATCTATATTATTAAATATACAAAGGGAATTAATAAAGAATTGCTTCAATCATATACAAAAAATGATTCAAATGCAACAAATAAAAATAATCAAGATCAAAATAATAATAATAATAAATCAAATCAAGATTCTAGCCCATCGCCTAATTGCTAATTTCAAATAAAATTTAAAATGTAACTCAATTGTTACATTTTTTATATTCTTTTCAATAAAATCTCTCAATTATTATTGATTAAATTTATAATTTAATCAAAATACGTTTAAAAAATTTATAATTCTAAATATATTTTAAATGTGAGGTACTTGCAATGAAATCAAAGAAAATAAACTTATTAAAATTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAAAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCGTTTGTTAATGATGGCAATGTTCCATATGTCAATTTGCTACAAATGATAAATTCCTTAGATGGATTTTTTGATAGAAACTATATAAATTTTTCGACAAATATTCTATTTTCTGAATTAAAAATGACAACTAAATTTGGATATATTTCGTTTAACTGAAAAACAAATAAAATTACTATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAAGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATAAAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTACCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATAAAATCTAATGTGCAAGAAATTCCTCAAATTAGAAATGGCAAATTTCAAAACTCACTTATTCCCAATGACATAGTTGAAGCAAAATTTAACTTCACTGCCTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTCTACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCGACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACTTGAGTTTCAATGCCTTCATTTTATAATCACAAGGATTCTAGCATTGCAAAAGATTTCTATGAATATACCACAAATAAAAGAGAAAGAATTTATCAATCTACAATAAAATATTTAAAAAATAATAAATTAAATAATTGAGTAATTCAAACAAGCAAAGAGAACAGAGAATTATTTGAAAAATATGGTGTTAGATTCATCTCAAACAATACCGCAGTAATTTCGTTTAACTCATTTAAAACCTTTCAAAATTTAAATGATAATTCTACTAATAAAGGATTTAGCGAAGACACATATTTAGGGTTTGCTAATGCTATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAATATTATTGTGGATTTATCAACTAATAGTGGTGGCAATATAGGTGCCATGCAAAGAGGATTGGGTTTTATTACAGATGAACCAATATTAAACAAAACATATAATCGTTTAGATGAAGAAATAAATATTAGATCTGTTGAAGTTGATACAGATGGCGATGGAGATTACAAAAACGATGCTTATTCTGAATACAATTGATATCTTTTAACCGGAGTAAACACTTTTAGTGCTGCAAATACCTTTACTTCAATTTTTAAAGAAATGAAATTAGGCAAGATAATAGGACAAAAAACAGGCGGTGGTGCAGCATCAATTTTACCTGTTGTGTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATAATTTATCTGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGCGGAATAGAACCCGATATTTATTTAGAATACAAAGATTTTTATAATGACCAAAAACTATTAGAAATAATAAAAAATAAATAAATCAATACTTATCATCATTTTTATAAGGATTAAATTCATATTGTTCAATGTTTAAAAATATTGCAACAAAAACAAGATAAGAGTTTCTAAGCAATTATCTTGTTTTTTTGTATAAGAATATATTACAAAAAAATAACTAAAATAGAAATGTATATTTCACAGTAAATAAATTTTTCAAATTAATCAACCTTGTTGAATTTTTAAAACATCAAACCTACCTAAAACATTAGGTCAGTCAATTACCAAACCATAGTTTTGTTTAATTTTTTCTAATTTCTTAAAAAATTAAAAATAGAATTTTAACAGGCTATTGCAATTTAAAAATGCTTTTTTAGCTACAACGCATTTTTAGTAATGTAAAAATGAATTACATCAATATAAATAATTAGATAATAATTAAAATAAAAAACCTTTCATAGTAATTTTATCTACTAAGAAAGGTTTTTTAATTACTAATTTTTAATAATTAATGTTAGCAAAAATTTATTTAGCACTTTTTTTAGCATCATTTATTGCTCCAAAAACTTTTGGCAGTTTAGACATTAAAACATTTTTTAAATCTTTACTATAAATATTTGTGATTAGTTGCTTAAATATCAATATTATTCCCTTAAATTCATTAAAAATAATTTCATATTCTTGTTTTGTTATTTTATTTGAATTTACCATATCATTTAATTTCTTTTTTAAATCATTGCCGGATGGATCAGAATATTTTGGCTCTATAAATTTTTCTACATCAAATAATATGGGCCATCCACCCCAAATTAGCAATACCTTCTTTCCTGCACCTTTATTATCATATTGTGTTTGTTTTGGATCTTTACCTTCGAATAAATAATCAAATTTAAGATTGCAAAAGTAATCTAAAATATATTCTAAATGTTTTATTAACATGTTTTTTAATTCATTATATTTGCTTTCATTTGTTTTTAAAAATTCATGTTTTTCTTTAAAGCTATTCAATATTGGAGTTAAATATTCAATGAAAACATTAGTTCCATTTTTAAATTCTTTAAACATTTTTATAGCTCAGGCTCCAACTGTTTTTGATAAAACTCAGTCTGCTGTTAACTCTCTTAAACCAGTAGAAACTTTATCATTAATGCCTAATTCTAATAATACATTTAACATTTCATCATTGGTGTTAATAATTAAATTAATTAAGATGTTGTAAAGTTTTTTAATAGATTCTTTGTCGGAATTAAATTGACCAAATACATTAATTGCTTTATTTGCTAGTTCAAATAACGTTTTTTCTATTTCTTCGTTAAACTTTTTATATTGTTCAATTATTGCTTTAATTACGTTTTGATGAGCGGCTTGTTTTTGTTGTTTTTTTCTTGCTTCATTAATCTTTTTTTCAATATTTGTTTCAATATTTAATTTAACATTTTCTTGATATTTTTTAAATTCATTTGAATTTTTTACATCCGATTCTAAATTTTTAGTTATTTCACTTATATCCTTAGCAAATCCATAAAATTCAAATGTTCTTGATTTAGTAGACTCTATTGGATTTTTGCTATATTCTTTCAATAAACTTTTCTTCGTTTTTAACTTAATTTTATAAGTTAATTTTCCTAATGTTTTATCATATTCAACTGCCTCTATTTCATAGATATCTGCAAAATCACTATCTTTTAGATTAAATTTATAATCATTAATTGTAATATCTTTAACATCAATAGATTGCTTTTCAAAATCATTATTTTTAGTGAATTCCATTGTAAGTTTATCTAATAAATCGTCAAGCCTTTTTGGTTCATTTTTTAATTCTTCTTTTATTTTTTCAATGCTTGCTTTGTCATTGAAACCTGATTTAGTAAAGGTTTTTATTTCACTATAATCGTCTAATTTGAATGTTATTTGTATATTTATATCATTTGCATTTTCACTTGCCAGTAGTTTATAATCTTTTTGAATTTTAAAATCATCAATAATATAAGATTCTTCTCCGCTACCAAAATTAATTTTTTCTCTAAAAAAGAAATTGTAGCCTTTTTCTATTGATTCATCTATTGTCATTGATTTTGCTAATTCTTTTTTGGTGTAATCTTTAAAATCAAAATCAAAGTTATTTGCAAAATTTTTTAAATTTCGTTTATTCATAATATTAGCAATAGAAATTTCACCCGAGAATTTTTTAAAAAGTTTATCATTTGCTGATTTTATCTTTAAAATAAATTCATATTTTACAGTAGTAGTATTTACAAATTTATTTAATGACACAATAGCGTTTTCATTTTCTTGATTCAATTCAATAATTGAGTTTTGATTTAGAAAATCAGAAATACCTAATAACTTAGCAATTTCTTGTTTATTGTTAGCAATTTTATTTTTAAAATCTGCTTTAGTATAAACACCATCAGTTACATAAGTTGATTCTTTTGAAATGCTTAAGTTATTAATTAAATTAAATGTGTAATTTTCATCACTTAAGAAATTGAATGTAACTGTCTTTTTGTTTGATTCTATTGTTCCATGTTTAAATGTTTTATTCAATAACGAAAAGGCTATTTCTAATTTTCCATTGGCATTGGCATTGATTTCTGAAATATTTAATTCCAACCCTGCTAGTTCATTACTTTTTTGCTTGTTTAATTCAAAGTCATTTTTTGAAATCATTGAAGATTTTTTAGATGTTAAATATTCATTATTTTTTAAAGATGTAATGTTTTCTTGTTCAACTAATGTATCCAATCTTTTTCGTTCAATATTGATTTCATTTTGCTTTTTAGTTAAGAAATTCGTAATTTTTTTTGAAATTCTCGATTCAGAAACAATAAAGGTTTTAAATTTTTCTTCTTTAACTTTTATTGTTCAGCTTACTGACAAAATTCCTTGATCATCATCTGCAATAATTGATGGATTTCCTTCAATAGTATATTTTGAATCGCTTATATTTAGATGAATTTCTTTACCTTCAACATTTGAAGGTAATATATTTGACTTATCATTCTCTTTATCAAAAACTAAATTTGCTTCTATCTTATCATCGCTAATTAATTTATTCAACTCTTGCTTGCAAGCCTTTAAGTATTGTTCTTTTGTATAAAAACCTGACAATGTTATAGTTTGTTCATTTGATTTTAAATCTTTATATTGCTTTTCTTTTGTTTTAACAACAAATTTAATTTTTAAGCTTCCTTTATCATCATTTGATTCACTTATTTCTAAAATTTCAATATAAAAATCATTGCTAATTTCTGTTTCAAAATCGTTTTTTTGAACATCAGAAGCAAATGTTTTGGCAATATCTTTTGAAGAAATTAATCTTAATGTTGTTGTTTCTTTGGCAACGTTATTTAAAGATTCTATTTTTTCATTTTTATCCATTGTTTTAGTTTTATAACCACTTAATTCAACTTCAATAAGTTTGCTTGATTCGCAATATTCATTAATTTGTTTGTTCAATTGCTTGATTGCTAATCTAATACTTAATTTTCCACTTTCATCGTTTGGAATTAATTGCTTTACAACAACTTCAATATTTTTAAAATTATTATTTGGCTTTGGAATTTCAATATTTAAATCTGATTGTTTGATTGAAGATGCAAATCTTTTAATAGCAGTTTCAGGTTTCTTATCGCTTACTTTTATATTTAAATTTCCTATTAATTCATTTAAATAAGCAATTGTTTCATTTCTTTGGTTATTAATAAATTGTGCATTTGTTAAAAAATCTATCAATTTTACTGTTTTGATTTCCGTTTTTAAATCGCTGAATTTTGGATTAGTACTTTTTAAATAAAAACTAATTAACAATGTTCCTTCTTCGTCATTGGCTTGCATTTTTTCAATAACTAACATTGCTCCATCAATATTTTTTTTATTGAAATCAACATTTTTGATTTCCGAAGGTTTTTTGCTGTGCTTTTCAGTCGCTAAAAATTCGTTCAGATCAATTGAATTAACTAAATTAGAAAGTTTCTTTAATGTTTCTTGTCTTTCTATGTTAAGTAATGTATTAAATCCCACAAATTTTTTAGTAACAATATTTGATTTTAGATTCTTTTTATTGTCATTGTATCAATAGCTAATAGTTAATTCACCTTTTTGATCGTCATAACTAAATTTTTTTTGCAATTCATATTCATTATTTAGATTATCGAATTTGAAATTATCTAAATTTTGAATATATTGAGATGGCAATATTGTATTTATATTTTTTTCATTTATTGATAAATTAACTTTATCAACTGCGATATTAAGATTTTTTAAAAATGATTTAAAGCCCGTAATTTTAGTTGTATAAACCTTTGATTTAATTCAATATGAGGCGCCAGTTTTCTTATCAATATATTGTTTTTCAAAATAATAATTTAATTTAACTTCCCCATTTATATCATCAAAAATTAATTCACCATTGCTTCGTAAATAAGTTGAATTTAGTTTTTTAGACGTTTCAAAATCACTTCAATTCAATGTTGAAGCATATGTTTCTGATTTATTAATTTGTTTCTTTAATTTTAAAGATATCTCATTTCCTAATTTATTTAAAGTGTTAGCTTCTTTTTGCATATTAGCTATATTATTCAATGAATTAAAACCATAAAATGTTTTTGAAATAATTTTTGATTTTATATCTTTGTCTTTTTGTACATAATAATATTTAACTTCTATTTTGCCTTGTTCATCGTTTGCGATATATTCAGTTTTTAATTCATATTGGGAATCGATGTTTTTAAATGAAAAATTGCTTTGCAATTTAATATATTGCGAAGGCAAAAAGTCTTTTTTATTAAATTCATTTATTTCTAGATTAACTTGTTCAATTGACGAATTTAAAGGAGCAACCAACGCTTTAAATCCTGTATATTTTTGAGAAAACATATTTGATTTTATTAACAGTGGTTGTTTTGTTTTAGAATCGATTATTGATTTTGTAAAATAATAATTTATAATAACATTTCCGTTTACATCATCTGCAATAATATTAGAGATTTCAAATGATATTTCTTTATCAAGATTATCGACATAAAAATTTGATGAATTAATTTCGCTTGGAAGTATCTTATCCTGTTTAATAGAAAGTTTTAAATTTGGTTTAATATTTCTTGTTTGTTCTTTTAATTTTGTTGTTTCAGTTTCTTTATCTTGCGTCTTGTTGCAGGCTGATGAAATGACTCCTGATACAAGTAAAATGGCAGTAGCACTTAAAATGGAAATGCTTTTGATTTTTGTATTCTTTTTCATATCTATTTTTAATATTTCTTTCTTTTTTAATTAGATAAAAAAAGCATAAATGGAAGACTCCCATTTATGCTAAAAGTAAAAATTATTTTAAGATTTCTAACACTTTTGGTAAATCTCTCAATGATTGTTCAAATAAGTTAATTGATTTTTTTAGACCTTCATTTAAAACGTTGTAAATTGGTCTAATTACGCCTTTTACTTTTTCAATATCTTCATCAGACATTTTTACGGATTTTAATACCGCAACTGCTTCTACCTTAGCGTCAGATAATAATGTAATTAATTTTTCAAATGTTTTTACATATCCTTCTTCAGCCAATTTGCTAAAATCAAATTCTTTTAATTTCTTAGCGGTTGTTTCAAATAAATTATCTTTTCCAGCAAATTTATTGTCAACAGCTGTAATTAAGTCATTCAATTGAGCTATTGCTTTTGTTGATTCGTTTGTTTTTAGTGTTTCAAAAATTGATTTTGCATATTTAGAAATATTTTTTCCAAATGCAACCAAGAAATCTGCCAACCCGTTTTCTTTTTTAATTAAATCAGAAATTTTTTCTTTTGTTAAATAATTTTTAATTGTCATTAATCCATCAGAAACTTTTGTTTTATTTGTGTTGTATTCTTTTAATGTTGACAAAATAATATCATCTAAAATTTCATTTAATTTAGAAATTGCTTCATTATATGTTTTTGTAATATTTGGCAATTCTTTTTCAGAAACTGCAGCTTTAATTTCTTTATTTTCAATAATTGGCTTTAAAATGGCTTCTGATTTAGTTTTTAATTCTTCACGATATTTCTTTAATTCACTTTCAAAATTTGCTTTAACTGTAGCATCCGCAATACCTTCAGTTGCTTTTTGAAGTTCGCTTTTTTCTTCTGGTTTAACTTCTGGTGTTGTTCCTGGTTTTTCACCTTCAGGCTTCTTTTCGGGTGTTGTTCCTGGTTTTTCACCTTCAGGATTCTTTGGATCTTCTGGTTTAACTTCTGGTTTAGTTTTTACGCAACCTGCAGCAACCAATGGAGTTGAGGCTAATAATGGAAGTGCTACCCCCATAGTTAATAAAATTTTTTTGGATTTTTTCATTTTATAATTTCCTTTCTATATAAAAATAAAATATATTAACTTCTTATAATTTACCATATGGATTGTTTAAGAATAATTTAATAAATTGGATTTCTGAAAAAAAAAAAAAAATGAACCAAAAAGTAATTTTTTACTGGTTAATTATTAAATTACTTTTTAATTTAAGATACTTTTTATAAGCAGGAAAAAATTTTATTATTTTTAAATTTAATGAATAGCTATGCTTTAAAGATTTGTAATATTTAAAATATTAAAAAATGTCTATATAAAAATTAATTTTTTTTAATTTAACTTATAATTTATATTAATTATTTATATATAAATATTTTAAAACACTATATTTTATAGGAGGACACTATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAAATTTCTTTATTAGCATTAAAGCTATTAATATAAATAAGGATTTAACATGATTAGAGAAAAATTAAACAAAATTTTAAAAGATAACGGGATTGATGCAATTATTTCAGAAGCACCTCAAACTCGTTTGTGATATGCCGGAGTTCAAACAAGTGATGGATTTCTTGTTATTGAAAAGGAAAAGGCATATTTATTTGTTGATGGAAGATATATTGAATATGTAACCAAGAATTCAAAAAATGTAGAAGTTGTATTATTAAAAGGCACTTCATTTAAAGACTTTTTGGCTAAAAAGAAATACGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTCGAAACATTTGAAAACTTAAAGTCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGCAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGCACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGAGCAAGTGATATCACTAGAACATTTTTCTTTGGAAAACCAAAGAATCCAAAACTTGTTGAAATCCTAGATATTGTTAAAGAAGCTCAAAAATTAGGAAGAGAAGCCGTAAAACCAGGTATTGCTACAATAGAAATTGACAAAATTTGTAGAGATTATATTGAAAGCAAAGGATATGGTCAATACTTCACACACTCAACAGGACATGGAGTTGGAATCGATGTTCATGAACTACCTGGAGTAGGAAGAAACCGCGGAGATGCAATATTAGAAGAAGGTATGATTATTACTGTTGAACCTGGTATATATATCGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAAAGTATTAAGCAGACCCGAAGATTATGAAAAATAATTAAATAAAAAAATGAACAGCTGGAAAATCCGGCTGTTTTTTTCGCTATATTTTCTTTATATTTAAATATTTCTAATTATCAATGATAAAGTTAAATCAAGGAGAATAAATTATATGAACAAAGAAGAATATTTAGCTTATTTAAGTTATATAAATAATGGCAAATGGCACCAAATCTATAATGATTTAAAATCACAAATAACATGTCAAAAAACAAAATATGCTGATTTTTGTAGTGACCAAGCAATTAAAAACAAATCATACATAACAATTTTTGATAGTGATTATCCGCTATATTGCAATAATGTTTGAAATCCACCGTTTGTCCTTTATTATTATGGAAATAAACAACTATTAAAGCATAAAATTATTATGATAACTGGAAATATTTCTATTTCTAATATTATTGAGGTTAAAGATTCTATTGAAAATTTTAGCAAAGAATATGTCTTCTGCAATGAAGCATGAAAAGGCTTAGACATACAATTAATTGACTATTTAATTTCAAAAAATAAAAATATTATTATTATTTTAGCATGCGGAATTGAATACGCTAAAAATTCTTTGCCTAAGAAATGATTCGATTATAGCAATTTGCTATTCATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGAGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGCTATAAAAGCACCATGAATAACCTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCAAGAAAAATAACTTCAATAACTGATTTAATATTAAAATAGAATATTATCTTTTTTATTATTCTCTAAAAGCATTATTATTTCATCTTTGTAAGGACTTTTATTATTAATAAACGGTGTTTCTAAAATAATAGGAATATTATCGAAGTCTTTATCATGAACAAATTTTTTCAATGTTTCTAAACCTATATGGCCTTTATCAATGTTTGCATGACGATCTTTTTTTGATCCGAGTTCATTCATGGAATCATTTAAATGAATAACTCTAATTAAAGGCAATATATTTTGTTGTTTTATATAAGATTTGAAATTTTCATAATCTTTTATATCATACCCCGCATCTCAGGCATGGCAAGTATCAAAACATATTGCAATTCTTTCGGAATTAACGGATTTTATCACGTATAATAAGTCATTGAAATTGCTACAAATTTGACTGCCTTTACCTGACATTATTTCAAGGCATATTATTACGTCCTTAGTAGATTCAATTATCTTTTTAAGAGATTGAATTAATGATGCTAATGCTTCGCTTCTGCTATATTCAACTGATGAACCAGGGTGTAAAACTAGCAATTTAGCGCCAAAACTATTCATTCTTTTAATTTCACTACATAAAAATTTAATCCCAAAATCTGCCTTTTCTCTATTGGCTAAATTTACTATATATGGGGCATGAACCACAATATCAGATACAGGAATAATACTATTGCATTGCTTTAAATATTTTTCAATATTATACAATGAACTATCAACTCTAAAAGCACTTTGTGGTGCTCCTAAGTAAATCATAAAACAATTCTCGCCATTATTAATAGCTTCATTAAATGAACCTATTAAATAATCAGGTTTTTTAAATGATATATGGCTTCCTAATTTAATCATAAACACTCCTAAATTTATAAAATTGTCTAATCTATTAATATTTAATTATATTAAATTATAAAGTTTAAATGCTCTTTTTAAATCATTCATAAATTTAAAAATAAAAAAATAAGGCTACACGTAATTTTTGATTTATAAACAGCCTTATTAATTGTATGTGTTTGTTTTAGTCTAGATCATAGCTATCGCTATTAAAGAATTCCTTCAATTTTCTACGTTTTTGAGGATGCTTCAATTTACGCAAAACTTTTGTTTCTAATTGTCTAACTTTTTCTTTCGAAATGCCTAATTCTTTTGAAAGTTCATCTAAAGTATGAGGTTTGTATTTGTTTCCCTTTAAGTCGTTAATTCCGAAACGTTTTCTAATTAAATCCCGGTCAGCTTGATCGCTTAACCCTTCTTCAATCATTTCATTTATAATAACACTTAACTCTTCATGACTTGCATACGAAGTTGGACTAATTACGCTTTCGTCTTTAACAAAATCTGAAAAACTAGAATTTTCTTCTTTTCCAATGTTTTTATCCAAACTAATTGGATCGATATTAATTTTTCTAATATATCTTACTTTTTCAGCAGTAAAATCTCCACCGTATTTTTTAGCAATTTCTTCATCTGTTGGAGGATATCCATTTTCTTGTTGCAATTCACGTTCAATCTTCAATATTTTATTTATTGTTTCAACCATATGAACTGGCACTCTCACAGTTCTAGCTTGATCGGCTACTGCCCGTGTTATTGCTTGTCTAATTCATCAAGTAGCATATGTTGAAAATTTAAATCCTTTTGTGTAATCATATTTTGAAACTGCCTTCATTATTCCGGCATTTCCTTCTGATATCAAATCAATAAAATTCAATCCACGATTTTTATAACGCTTTGCATTATTGATTACTAAGCGGAGATTTCTTTTAACTAACATATCTCTAGCTTTTTTATATTTATAATAATTATTTGCTTCTTTAGCTTCTTGCATTTTTTTTGCTAGCGCTTGTTCTTCATTTGTAGTTAGCAATTTACCATATTTGCCAATTCATCTCATATATCATTTAACAATGTCGTTGGTTTCAGTTAGCTTATTAGTTAATTTTATATCATCTTGATCATAAACTTTTTCTTCACTCAACTTTATATCTTCGTCATTGTATTCTTTTAGCAAATTGTTAGCATCGAAGTCATCGTATGATTGTTTTTTACCTTGCTTGTGGTTTGTTTGATAATCGTCATCTTCATATTCGTCAAAATCTTCGCCGTAATAATCATTATATTCTTCATCATCAAGATCATTAAATGCCTTTTTAACAAAATGATTATCATCTTCGTCAAAAGACATTTCCGAGTCATCTTCTTCCTTGCTTCTAATTGAACTATCGTCAAATTCTTGATCAATATTTATATCTTCGTCAAGATTTTCTTCTTCCAACTCTTTAATTTTTGAAGGGTCAAAATCTTTTTCTTCCTCTTCTGCGTCATCGTCATCATTATCATCATCTTTAGTTGTTGCGTGAGCAACTTCATCATCTAACGAATTTATGTCAATATCTTCAATATCTTCGTCATCAGTATCAGATGAGACTAGATTAGCTGCAAAAAGGCTTTCAAGTAAAGCTTCTGCTTCTTCGTCATCAACGTAAAGGTTCTTTTTGTTTAGGAATTCAAAAATTTCATCTTGAGTAAAAGCCTTTTTATCCTTATTTTTCTTTTGGACTTTTTTTAACTCGTCTGCAACAATTTTAGTTATACACTTAACTGATGATTCTACATTATTTTTAGTATCTTGCATAATTATTTCCTTCTGTCCAAATTAATTATTAGGTCTTTGGATTGGCCTGTTTCTTTCATTCAATTATTTAAATTTTTATCTAGTTCTTCTTTTGTATATTCTTTGTTTTTAAGACTCATAATTTCCTTGAATAAATCTAAATATATTTGTTTAGCATTATCTTCAAGATCAATATTGTTTGTAATATATGATTCAAAATTTGTTTCATCTATTGATTTATTATTTTTTATGCTGTTTATTAAAAAACACAAAAACATTTTATAATTTTGACATCCGTTTGGAAATGCCATTCTAGTAAAATCAACAAGTTGATAGTTAGCAATGTATTGTGGGTGCTTTATAATGGTTTTTATTATTAAATAAAATACTAAATTTCTACCAGTTATTAATTTAGGCTGTTGTATTAAAGGTTCTTCGAAAAAATCAAGTCTATCAAACGAGGGATCAGAATATGCAAAATTAAGATCTTCGTATTCTTTGTAATTTGAGTTTTTAGAATAATTTTTTTTATTATATTCATTATCTAGACTAGGTTTTAAATTATTTTTTGAAATAGGATGAAGTGGGAAGTTTTCTTTACCATATAAGAATAAATATTTGTTTCAAGTTTCAATTGAAAAAATTTTATTTTCAATCAATTTTTCTTTTAATTTCAATATTAATGGTTCTTGCAAATAAATCACAAATTCAAACAATTCAGCATTTAATTCAACCTTTCTTGATTCGTTGGTTTCCAATTGAAATACTTGTTTAAATTCATTGAATAAATACATAAATAAATCGACAGGACAACATGCTATCTCAGACAATGTTTTTCCTTCGTCAATATTATATAATTCGTCAGGATCTTTGCTATGTTCATTCAAAACAAAAGAAACTTTTAAATTATATCTTTTTTGATTTGCTAATATTATTCTTAAATTTTTCTTAGTAGCATTTTTGCCGGCAAAATCATTATCAAAAAACAAAACAATATTACAATTTCTTAAAGATTCTAAATTTTGTATAGATAAAGCTGTGCCTAAAAGTGCTACTGCATTTTTTATGTTTATTTTATAAAGAGCAATGCAATCAAATTGCCCCTCAACTAAATAAACACTTGATTTCTTTATAATTTCATCCAATGCATGATAGTAATTAAACATAACTTCATGTTTTTTAAAAACTATTGTTTCAGAACTATTTAAATATTTTGGTTCAACGTTATTATAAATACTTCTTCCAGAAAAAGCAACAATTCTTCCATCCTTATTTTTGATTGGAAACATCAATCTATCATTAAAAAAGTTATCAAAATTGTTGCTTGCTATCAATGACGAATTGGCTAAATTAAATGATGAACAATTTTTAGCAATTAGCATATTACACAAATTTTTATCTTTATCAGCAAAGCCAATTTCAAATTCCTTAATAAGTGGTTTTGTTAACCTTCTTTTTTTAAGAAATAATTTTAATTGCTCATTTTGATTAATTTCTAAAATTTCAAATTGAAATATATCATTTGCTATTTGATTTATATTGTATAAATCTTCTTGTTCTTTTGTTGTAGTAGTTATACTATTACTAAACCTTGTTAAATCGATATTTAAATTGAATTTTGAAACAAGCTCCTTGATACTTTCAAGAGGGCTTTTTTTAGTATAAAGTTCAATGAAAGTTATTAAATTTCCGCTTTTAGAGCAACCAAAACATTTAAATATTTGTTTTTCTTGTGACACAACAAAAGAAGGAGTTTTCTCACCATGAAATGGGCAACAAGCCACATAATTCTTTCCTTTTTTTGTCAAAGAAATATATAAAGAAATAACATCAACAATGTTTAACTTTTGTTTAACAATTTCAAATATATTATCTTCTGCCATTTAGCTCCTTTTATTTTTTATAATTTTCAAAAAACTTAATTAAGTTGTCTAATTCAACTACATGTTGTTTCATAGAATCGCGTTCTCTGACAGTTACTGTATTTTTTTCTAATGTGTCAAAATCTATTGTTACTGCAAATGGTGTACCAATTGCATCTTGACGACGGTAACGTTTTCCTATCGAACCGCTTTCATCATAAATTAATCTTAATTCAGTTTTTTGCAATAAATTATCAAATATTTCTTTAGCCTTAGAGTTAAGTTTTTTCATTAATGGAAAAATCGCAACTTGATATGGTGCAATATTGAAATCAAGTTTTAAAACAATTCTTACATCATTATTTTCTAGTTCTTGTTCTTCATATGAATCTACAAGCAACGCAAGCAATAAACGATCTAAACCAATTGAAGGTTCAATAACATGAGGAATTATTTTAGTTCCATCGTCTAACAAATATTCAAGAGATTCATTTGAATATTTCATATGTTGGTTTAAATCATAATTGCCACGATTTGCAATACCAAGCAATTCTCCTCAACCAAATGGAAACAAATATTCAATGTCGCTAGTGCCTTTTGAATAATGGCTTAATTCTTCTTTTTCGTGTGGTCTAATTCTTATATTTTCCGACTTTAATCCCAGAAGTGTAACAAAATCAACGCATTTTTTAATTCAATAATGGTAAAACTTTTCATCTTCACTAGGATTGCAGAAATATTCTAATTCCATTTGTTCGAATTCCCTTGTTCTAAAAATAAAATCCCTAGGTGTAATTTCATTTCTAAAACTTTTACCAATTTGGCCAATTCCAAAAGGTAACTTAGCTCTAGTTGTTCTTAAGACATTTTTAAAATTTATAAAAATTCCTTGGGCTGTTTCAGGACGCAAATATGTTTGATTTGCCGCTGTATCAATTGCACCCATTTGGGTTTTAAACATTAAATTAAATGGCCTAATTGAAGATCATTCAGTTTTTGAACCATCATATTCCTTAACTTTTTCTTTAATTATTTTTTCAAGTTCTTCAAATGTTGCTTTTTCAACATTAATTTCTGGAAATAAATCTTTAACAATATGGTCTGCCCTATAACGTTTTTGATTTAGTTTGTTTTCAATTAAAAAATCTGAAAAATTGTCTAAATGCCCACTGGCTTTTCAAACTTTTGGATTCATAATAATTTTTGAATCAATTTGAACATTTCCTTCTTTGGCAATAAATTCTCTTACTCAATATTTCTTAATCGCAGTCATAATATTAACTGCTAGTGGCCCATAATCCCAGGTGTTTATTAAGCCTCCATAGATTTCTGAATTTTGATAAACAAAACCAGAAACTTTTAAGTGATTTATTATATTTTGAATATTTTTTTGTATTTTATCTCCCATAAAAACTCCACAATTTTATTTTATATAAAATAACAATGAAATTTTACCATATTTTTCCTTTTTCATTATTCAAAAAATAAAAATGATCATTGCTTTTTTTGAAATTGTTTCAATAATATATTGCTTTGTTAGTATAATAATTACATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAATTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCGAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAAAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCCGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACAGATTTCTTTACTATGGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAGGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATCACAAAATTAGATAGTGATGCTCGTGGTGGCGCAGCATTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATGGGCGATGTTCTAACATTAATTGAAAAAGCCTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTGATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCTGGAATGGCAGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTTGAGAAAAAAAACCCACGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAAGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAACAAATGAAAGAATTGGCAAACGGAAATCTAAAATTAAAAAATGGCTTGGATGAATAAAAAAAGTGCCTATTGTTTGGCACTATTTTTTTAACTAAATAATTAGCCATTTAAAATATATAATTGCAAAATAATAACTAAAAAAATTCTTTAAAAATAAAAAAGCACTTATGGGTGCTTTTTATAATTTATTCTTATTTTTAATTGAAATTAAGACTAATATTGCTTTTGATATTTTTAAGCTTGATAAAACATATCTATCAAAAATAATAATATTTCTTAATTTAAATATTGAATTTAATTTTTTAGTTTTTACAATTAGGTTATATGGAGAAATAGTTTTAATTTTATTGATGAAGTGATTAAATAAATTATTTAAAAAATATGAATGGTTTTTAAATGTTTCTAAAATATATTTGATATTTTCAGAGGCTATTTTTTTATCGCGAGGTTTTAATTCTTGACAATTTTGTTTTGAAAAATAAGTATTTAAAATGAAATTGATTGTTTCTTGCATTTCTTCATCTCTTGAAATTTCAATAAATGCCTTATCTAATAAATAAATTAAATTGTATTTATTATTTACTATTAAATTAGTTCATAATTCATCAAAGGTTTTTAATTTCGCATAAATATCGCTATAGTTTAAAAGTGTTTCTACTATAAAATAAATAAATTCCTTAAATGTATTCAATTGAGACAAAAATTTCTTAATTTCTATATATTTTTGATTATCGTTGAACGAAAATAAAAACATTAGCAAATCTGTATTTAATGATTCTTTTGCAAACGTTAGAGCAATTTTTTTAATTTCTTCTTTGTTTTCTTCTATTATTCCTGCACTAAAGAATTGTTTAAATACATCATATATTAAATATTGATAATTGCCTAGAATATAATTTCATTGTTCACGCAAATCAAAATTAATAATTGTTTTTTTAGTTAAAAATACATCTTCAAGTCTTGATTGTAATCTAAATAATATATCATCAATTTTTCCATCTTTAAATAATGTTAAAAATATAGCTTTGCTATTTGTTTCGTTGCTTAAAAATGCATCAATCAACTTATAAAATTCATACTTAGTTTTTGCACCAAAAAATTTATTTTTTAAGTAATGTCAAGCAATGTTATGAATATCATAAGAGGAATATCTATTTATTAATTGAATAAATTCTGAATTATTTAAATGTTTATATAAATATCTTTTTAGAGATATTTCTAATTTGCTATCTATTAATATATTGAAATTCTTATTAATTCCATAGATATTTTCTAGTAGGTCTAAATCATTGTTGTATAGTGAGAATAATTGTTGATCTTGGTTTTCAAGTGTTTGATTTCAATATTTAGAATCACTACAATATTGAATTAAATTAGACATTTTAAATTCACTAGTTAAACTTAATTTAGCAAGTAAATCATATGCAACTTTTTTATATCCTTTTTCGGTGCAAAACCAACTAAATAAGTTTTCATACAAATAATTTTGGTGTTCTTTTCAATATTTTTCTTCATAAATATCTACATATAAACATTGGCATTTTTTAGCTACTTCTTTAGCGACATTGTTTAATAAATCTTGTATCATTTCAACCAAATCAAATTCATTGTTATATGAAGAAGAAATGTAAATCGAATTAATTAAATCTTGCAAGGCCATAAATGGTTTGGCATAATTTGTTACCACGATATTTGCTAGTGGATTAATTTTTTGAATATATTCAACAAGCTTTTGATATTTTGAAATAATTTCTTTGCCGATATTTTCAAAATCTTTTTTTATTTCAATAATTAAATTGGTTTTTGTTTCATTTTTTGCTGTTATTAAATTAGTTAATTTATAAAATGGCATTTTAAAAATTAAATCGCTAAATCCAGCTGTAATTGTTATAAAATTTGCCTTTGAAATAGTTTCTTTATAAAAAGAAAAATTTTTTTTAGAAATGTTTCAATTTAAAAAAAATTCATCAAAAAAATTCTTTGAAGGATTTGAAACATGCCAATCAATTGATTGAAGCAAATCAAGACTTTTTGAATTGGCAATTAAATCATTTTCATTATTTGAAGTTAAAGCATAAATTATATCTAAATTACCTGCTGGTATTGCTAGATTGTAAAAACTTGTTAATCTATAGTTTAAATCTCGCAATTTTAAGGCTAAAAATGATGGATAACCCAAACCATCTATATTACCATTTGATAATTTTCCATTAGTATTAAACCCTACTTTGCTATTAAATCCCGCTGCAAATGAATCTCCTAAAGCAACATATTTAATTTCGTCATTTAAATTGTATTTTTGATTTTTAGCCATTTTGTTCCTTTTCTTGACATTGATCATAAACAATATTATTTAAATTATTAAAATAAGAGATTAATTGAACTATTGCTTTTTTTATATCGGGTAATTGATTTTTTAATATTCAATTGTTATTTTTAGCATATAAACTCAATTGGCCAAAGATTTCTTCTTCATCTTGACTTGGTTGCAATTTTATATCAATAATTTTTTGAGGATAATACTTAGATATTAGGCGGTAAGTAGCAACAATAACTTCATTTATATTTAAAGCTTCTAACTTTATTGAACTAACTACAGCTAATTTGGCGCCGGTTTCTTCATCTTTTAGTTTTGGCATTAAAAGTCCCGGTGTATCCAAAAGTAAATAATTTTTGCAATTTATTCATTGCTCTGACTTTGTTACTCCTGCCATTGCACCCACTTTTGCAACTTTGGAACCAGCCAATAAATTTATTAAGGTACTTTTACCAACGTTAGGAACGCCAAGCACGACACATTTTAATGCGGGTGTTAGCTTATGTTTCTGTGCATTTTTTTCTTTTACTTTTAAATAAATATTATTAAGAGCTGAATTTATTTTTTTATAGGCTAATTTGTCTTTCAAATTAGTTGTTACAACAACACCTTTCTTCTCATATTCATTTATAAAAGGTTTTAATTTAGATAGATCTGCTTTGTCGCTTTTGTTTAATATAAATAAAATTGGCTTATTATTAGCCATCTTATAAATATCGTTATTAAACGAAGTAAGAGGTAAGCGTGCATCTAAAACAATTATAAATAAATCAAATAATCTTATTTTTTCATTTAATAATCTAATGGTTTTGGCCATATGACCAGGGAATCAATTAAACATAAAATTTATCAATCTTTCCTTAAAATAAAATCCTTCCCTTTTTGACTTTCAAATTTTGATAGTCCGGCGAAATCATTTTGTCTTAATACTTCAAATAGTACTATTGCAACACTATTGGCTAAATTTAAACTACGACATTCATCAAACATTGGGATACGCAAACAATTAGGAATATTTGTTTGCATAATTCTCTTAGGAATTCCAGTACTTTCAGTTCCAAACATAATTCAAAGTTCACCATTTGTTTGCAATTCTTGTTTGTAATCAACATCTACGTAATTTTTTAAACCGTATCTTGTTACATAATAAATATTTTTATCACCATATTTTTTAAAAAATTCATCATAATTTTCATGAACTTCATGTTGAATTTCAGTTAAAAAGTGTCCAGCAGCCTTTCTTTTCATTCAATGAGGATGAAGATCAAAGGCAATCGGTTTAATAATATGCAATTTTGCATTGCCAGCAAAGCATGTACGAATTATATTGGATGTATTTGGGCTTATTTCGGGTTGGTATAAAATAATGTTAATCATGTAAAGTATTTTACTACAAATTGGCATTAAAAATAGTAGATATTGTAATTTTAATTATTTTAATAATTAAAAAATAAATTTATATAGATCAAATTCAAAAAAGCAGATTTTAAAAAATTTATAACTAAATATTCATTTTTATTGTAGCTACATTTTTATCTTAATATATAAAACAAAATAATTACAGAAAATAAGAAAAAATAGCGAAAGAATCGCTTTTTTTCTAAACTAAAATAAAAAGCACATTGATTGTGCTTTTTGCCAAAATGAAAATTCAAGTGCAACACAAATTTGAAATAAAATTTAGTTGTGGGGCACTTGAATTTTTTTAAAATAAAAAGCTCCACAAACGTACCAAGGGAGCCTTTATGAATTATAACATACATAAATATTTTCATTTGTCATTAGAAGAGAGATTTTTTATTCAAAGATCGCTGGAAAATAATTTATCATTACGAGCAATTTCAAGACAATTGAATAGAAGTGCATCTACTATTTCAAGAGAAATAAAAAGAAATTCAAATTTTAACGAATATATATTTTTGAGAGCTGATAAAAAATCAAAATTTAGAGCATATCATAAATACATGTTTAAGTTCAATATAAATTTTGAATTTGAGGAATTTTCAAAAATATTTAAGTCTAAATATGACAAAAAATATTTTGGCGTTAAAGCAACAATGAATTATATTGAAGAAAACTTTAATGTTAAAAAACCAACAATGCGAACCGTTTTCAATTGAATAAACACAAATAAATGAAAAATAAGAAAATCCGATAAATTAAGGCAATATTATAAAAAAGGAGGCAAAAGAACTGCTTCGGTTCTTAGTCGTCTAATAAAAAGTGCAAATTATGTATTTCCAATATGAACAAGACCAAAAAATATTGATTTAAGGTTGGAATATGGGCATTGAGAGGCAGATTTGGTTCTTGGAAAAAGAGCAAATGGCTATAAGAATATATTAACTTTAACAGAAAGAAAAACAAGAGTTGGATTTGCTACTTTTGTGTCATCTAAATCTCCTTTTGAAATCAATAGCAAAATCAAAGAATTAATAAAAAACAAAAATCTTATCGTTAAAACTATAACAATCGATAATGGTATAGAATTTGAAAAAATTGGCTTTCTAGCAAAATCATTAAATATTATTATTTATAGAGTGGAACCTTATGCTTCATTTCAAAGAGGTTCAAATGAAAATTGAAATGGCATGATAAGAAGAGAATACCAAAAAGGATTTAATTTTAATATTCTTACCCAAGAAGATTTAGACACTACAATAGCTAAAATAAATAATATGCCAAGAGAAATATTAAACTGAAAATCTGCATATGAATTGTTTATGTATGAAAATTTTGGCGAAATAATAAACTAGAAAAATTTAAAAAATATAACATGTTGTTTATCACAACGTTTTTTCTATGCAAAAATTTTAAAAATCGCATAGAAATTTTAATAACTTAATATAATAAAAACATCAAGCACAAAAGTGTTGCACTTGATGTTTCATTCTGGGAAAAAGCACATTGATTGTGCTTTTTATTTGCTTTATTTTCTGATGTTTAAAGCTTTGATGGTTTCTAAATATAAGTTGTAGTTTTCGTCTTTTAAGTGGTTTAGTAATGCTTTACGGTGATTAACTTTAGCAATGAATCCTCTCATTGAATGTAAATCTTTTTTATTTACTGCGAAATGTTCTTTTAAACTTTCAATGTCTTCAGTTAAAATTGCTATTTGTACTGGTAAATATCCAGTGTCTTTTGCATTACGTCCGAATTTAACAACTAATTCAGCTTTTTTTTCTTTTGATACCATATTTTTCCTTTCATAGCCTAGCAATAATCTTAATTATTACCAAGGGATAAAATTGTTATGAATATATTTTAACATAAAAATAAAAAAAATACGCAAATGCGTATTTGGTCAAGTTAATGAAATGGTGCGAACGAATGGACTTGAACCATCGACCTCACGATTATCAGTCGTGTGCTCTAACCAACTGAGCTACGCTCGCATATAAGGAGTTTTAAAAAACTCCATAATATTATAACACAAAAACAAATTAACGTTTTGAAAATTGTCTTGCACGTCTTGCTTTTCTTAAACCGAATTTCTTACGTTCTTTAACTCTAGCATCACGTGTTAAAAATCCTGCATCCTTTAATTTGTTTCTATATTCATTATTAGATGCTTCTAATAATGCTCTAGCAATACCTAATCTAATTGCTCCAGCTTGTCCTGTTAAACCACCACCATTAACATTTGCAAAAACATCAAATTGGTTAGCAGTTTCAGTTACTGCAAAAGGACTTAAAGCATCTTTTATTAAAATTTCTGAATTTAAGTATTCTTTTGCAACTATTCCATTAATAATTAAATTTCCACTACCTGGTAAAATATAAACTCTTGCTACTGAAGATTTACGACGACCTAAACCATAGTATCTTACTTTATCTGCCATAATTATTTAACCTCTAATTTAACTGGTTGTTGTGCTTCTTGTTTGTGTTCAGAACCTGCATAAACATATAAATTTTTAAATTGTTTACGACCTAATTTGGTGTGTGGAAGCATTCCTCTAACAGCTTTTTCAACTAATAATTCTGGTTTTTTTGCTCTTAAATCAGCAGCAGTAATTTGTTTTAATCCACCTGGGTATCCAGTGTGGTGGTAATATACTTTTATTTCATCTTTTTTAGCAGTTAAAAGTACATCTTTAGCATTGATCACAACCACATTGTCGCCCATATCAACATTAGGGGTAAAAGTTGGTTTATTTTTACCTCTAAGTACAGAAGCTACAAGTGCTGAAAGTCTACCTAAAGGTACGTTGGCTGCATCGATAATGAATCATTTTTTATCAACGATTTCTTTTCTAATAATTGTTGTTTGACGCATACTTTCTCTTTCTTATAGTTTGTTATTTTTATAAAATGCTTACCTATTATACCATATATAGTTATTATTAAAACATTTTTATTTAAGTTTTAAATATTTAAATAAAACCTGTTTAATTTAATTTTGAAAGCATGTAAATTAAAGCGGTCTCAGCCCTTAAAATCGTTTTTGTTAGTGAAACAACTTGAACATTGCAAGATATAGCATAATCAATTTCTTGTTGGCTAAATCCGCCTTCAGGGCCAACAATAATTAAAGCATCTTGGCAATCTTTAATTAATTCGTTATTTTTTTTGTTTTCATAAGCTAATACTTTATTTTTAATATCCAAGACACAAAGTTCTGAAAATGAAATTGGATCATGAAGTCTTGGGATACAATTTCTAAAACTTTGTTGGCAAGCTTCGAAAATTATTTTTTCATAACGTTGTTTTTTAGAAGCAAATGTTAAATTAGATTTATCAACATATTGGCTTTGAAATACATAAATTTCATAAGCGCCAAGTTCAACTGCTTTTTGCAATGCTACTTCAAAATGATTTTGTTTAATTAAAGGAATTGCAACACATAATTTATAGTCAAGTTCATTATTAATATCTAACTCTCTAACTAATTTAGCACTATTAGGAAAAACAAATTGACATTCATAAAACTTATTTTCATAATTTACAATAATATTTTCTTTATTAAAGCGCAAAACCTTAAAATGCTTTTGAATTTCATCACTAAAATAAAAAATTCCATCTTCTAATTTGTTAGCAAAAAATTTGTACATATAACTATTTTAATATAACCAACACAATAAAACAAAAAAACCCCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTACTAAACAATTAATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTCAAATTAATTGTTAGATGAAATAATTTTTGTATTTGTTTCGTCTATACCAATATATTGTCAACCTATATTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTTTTTGCAGCCAAAAAACGCACTATAGCCAAATTTTTTTACTGAAGCAGGAATAGTAATAGATTCAATGTTTGTATTGTAAAATGCCTCATCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTGACAGCCAAGAAATGCACTATCATCAATTTCTTTTATGTTGTTTGGAATAGTAACTTTTGTGGTTTCTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTATTGAAAAATTAAATGCCCTAGCGCCGATTTCTTCAAGACCTTCATTTAAAAATACTTCTTTAAGGTTTTTGCAATCATAAAACGCATAATCGCCAATTTCTTTTACTGACCGCGGTATAACAATATATTCAATGTTTATAGTTTTAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACCAGAAAATGTACTTTTGCCAACTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTATTAAATGCATAATCGCCGATTCTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACTAGAAAACGCACTTTTGCCAACTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTCTGTATTATTAAATGCATAATCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTTTTTAAGGTTTTTACAACTAGAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATTATAAAATGCCTCAGCGCCAATTTCTTTTACTGTACTAGGAATAGTAATAGATTCAATGTTTGTATATTTAAACGCCTGAGCGCAGATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATGTTTCTGCAGCCAGAAAACGCACCTTCACCAATTTCTTTTACCGAACCTGCAATAGTAACTGATTGAATAACACTATTAAAAAAAGCACGATTACCAATTTCTGTAATATTTTTGCCTTGATATTCCTTAGGAATTGTTACTTTAGAATCATTTCCAATGAATTTTGCAATTTTTCAAGTATTTGATTTAGTTAGTTCGAATTTGAATTTTTTATTTAAGTTTGATGAATCTTCTTCTTTATTAGGATTTTCTGGTTTAGGTTCTGGCTTATTTTGATCGGGCTTTAAATCATCTTCTTTGGTAGGATTTACTGGTTTGGGTTCTGGTTTATTTTCATCAGGCTTTAAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGATCGCAAGAAATAGCTGCTAATGGAAGTATAGTAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTTTTTTTATTTTTCATATTCTTTTCCTTTGTTATATTAATTATTATATTTTATATATCTATTTAAAGCAAATAAATTATTAAAAAAATAAAAAAATTCTTTTTTTGAATGAATTTGGAAGCATAAAAAAATGTGTGGTTAATTTCAAATAAGAAAAAAATAAGTTTAAAAATTTTTGAAAGGAATTTTATGGGTGGCAATAAAGAATTTATGTTAATTCAGCAAGAGGCTAAATTAAACAATGATTAATAGCCTTGTGGATGCATTTGAAAGAAATTTAAATTGGAAGAAAAATTATAGTAAAAAAAATCTATAACAAATTAAGCAACATAGCTATAAACAAAATTTTAAAATCAAGAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATAGTTATTAAATCTTTTAAAAAATTCCATCTTCTAATTTGTTAGCAAAAAAATTTATACATATAAGTATTTTAATATAACCAACACAATAAAATAAAAAAAACTCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTGCCTAATAATTATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTCAAATTAATTGTTAGATGAAATAATTTTTATCTTTGTTTTGTCTATACCAATATTTTTTCAATTTATATTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTTTTTGCAATTAGAAAACGCACTTTCACCAATTTCTTTTACCGAGCTTGGAATAGTAATAGATTCAATTTTTGTATTTGGAAATGCACTAGCGCCGATTTTTTTAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAATTACAAAACGCTCTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGTCTCTGCACCAATTTTTTCAAGACCTTCATTTAAACTTACTTCTTTAAGGTTTTCGCAATTAGAAAACGCACTTTCACCAATTTTTTTTACATTGTTTGGAATAACAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAACTATGTAAAATTATTGAAAAATCAAATGCTGAATCTTCTAATATTTTTATTGAATTAGGAATATTAACTAATTTAATGTTTGTATCTGAAAAAGCCTTAGCACCAATTCTTTCAAGGCCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCAACCATAAAAAGCACTTTCACCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATATTTGTATTTAAAAATGCATCAGCATCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGATTTTTGCAATTAGAAAACGAACTATACTCAATTTCTTTTACTGAGCTCGGAATATAAACCAACTCGATAGGTAGATTTTGAAATGCCTCAGCGCCAATTCTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAACCAAGAAACGCACTATCGCCGATTTCTTTTACATTGTTTGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTGAATTGGACAATGCGCCTTCTCCAATTTCTTTTACCGAACTAGGAATAGTAATAGAATCAATGTTTGTATCTCTAAATGCCTCATAGCCGATTTTTTCAAGACCTTCATTTAAAATTATTTCTTTAAGGTTTTTGCAACCATAAAACGCATTATCACCAATTTCTTTTATTGAACTAGGAATAGTAATAGATTCAATTTTTGTATCTTCAAAAGCCCAAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTCTGCAACCAGAAAACGCACAATCACCGATTTCTTTTACATTGTTTGGAATAGTAACTTTTGAAGTTTTTTTATCCAAATATCCTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATCAATTAAAATACTTGAAATATTTCAAGTAGGTAATGCACATTCCTCAATTTCTTTTACTGAACTAGGAATAGTAATAGATTCAATGTGTGTATTTCAAAATGCCCCATCGCCAATTTTTTCAAGACCTTCATTTAAAATAACTTCCTTGATATTTTCACAACGATTAAATGCAACATCACCTATTTTTTTAATTCAAGTTGGAATAACTACCTTTGTAGCATTTCTGTCTAAATACGCTAATATTTTTCTATCTCTCTTGTCAATTAGAAAATTATGTCTTATTTCAAAATTTGCGTTTTTTATATTAATAAAAGGATTTCAATAATCGAATGCAGCACCACCAATTTTTTTAGTTGAATTAGGAATAACAACAGAAGTTAATGAAGAACCAAAAAAAGCATATTGGCCTATTTCTTCAAGACCTTCATTTAAAACTACATCTTTAAGATTTTGAGAATATTCGAATGCATAATCTCCTATTTTTTTTATTGAACTAGGAATAGTAACTGATTGAATAACACTATTAGAAAAAGCACCATTACCAATTTCTGTAATATTTTTGCCTTGATATTCCTTAGGAATTATTACTTCAGAATCATTTCCAATATATTTTGCAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAAGTTTTATTTAAATTTGATGAATCTTCTTCTTTGGTAGGATTTTCTGGTTTGGGTTCTGGTTTATTTTCATCAGGCTTCAAATCTTCTTTGGTAGGATTTTCTGGTTTGGGTTCTGGTTTATTTTCATCAGGCTTTAAATCTTCATTGTTTTCTTTTGAATTTTGTTTTACTTTATGATCACAAGAAATAGCCGCTAATGGAAGTATAGCAGTTAATGTTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATATTCTTTTCCTTTGTTATTTTTAATTATTATATTTTATATATCTATTTAAACCTTAAAGTAAATAAATTATTAAAAAAATAAAAAATTATTTTTTTGGATAAATTTATAAAAAAATGGCTTATTTTCTATATTTTTTGTGTAAATTTTTGGAAGCGTAGAAAAATGTGGGGTTAATCCGAATAAGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTTCTATCATTCATAAAAAAAATAAGTTTAAAACTTAAAAATTTTTGAAAGGAATTTTATGGATATTAATAAAGAATTTATATTAATGCAGCAAGAGGCTAAATTAAACAATAATTATAGCCTTGTGGATGCATTTGAAAGAAATTAAAATTGGGGAAAAAATTATAGTAAAAAAATCTATGACAAATTAAGCAACAAAGCTATAAACAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAGTTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATAGTTATTAAATCTTTTAAAAAATTCCATCTTCTAATTTGTTGGCAAAAAATTTATACATATAACTATTTTAATATAACCAACACAATAAAACAAAAAAACTCGTAACTTTTTTATAAATTACGAGTACAAATTTTTTACTAAATAATTAATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTTAAATTAATTGTTAGATGAAATAATTTTTATCTTTGTTTTGTCTATACCAGTATTTTCTCAATTTATATTACTTGCATCGCCCTTATAAATTGCTTCTTTAAGGTATCAGCAACCAGAAAACGCACCTGCGCCAATTTCTTTTACTGAGCCAGGAATAGTAATAAATAAAATTTTTGCATACCTAAATGCATAATCGCCAATTCTTTCAAGGCCTTCATTTAAAACTACTTCTCCAAGGTTTTCGCAACCAGACAATGTACTTTCGTCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGCTTGTATGATAAAATGCCTTAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCATTAAGGTTTTGGCAACCAAAAAACGCTCTTTCGCCAATTTCTTTTACTGAATTAGGAATAGTAATGGATTTAATGTTTGTATGGCGAAATGCCTCAACGCCAATTTCTTTTACTGTGCCAGGAATAGTAATAGATTCAATGTTTGTATATTTAAATGCCTCGGCACCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTTGCAACCATAAAACGCACCTTTGCAAATTTCTTTTACTGAATCAGGAATAGTAACTTTTGTGGCCTCCTTATCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTATCTTCAATTTCAAAATTTTTATTATTTGAATTAATTGAAATACTTCGAATATCTCAAAAAGATAATGCACCTTCGCCAATTTCTTTTATTGAACCAGGGATAGTAATAGATTCAATTTTTGTACTTGAAAATGCCTCATCACCAATTTTTTCAAGACCTTCATTTAAACTTACTTCATTAAGGTTTTTGCAATTAGAAAACGCACCTTTGCCAATTTCTTTTACTGAATCAGGAATAGTAACTTTTGTGACCTTCTTGTCCAAATATGCTAATATCTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATATGAAATTATTGAAAAATCAAATGCCTCAGCGCCAATTTCTTTTACTGAGCCAGGAATAGTAATAGATTTAATGCCTGCATCTCAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTCAAAGGTATATGCGACCATAAAACGCCCTTTCGCCAATTTCTGTAATATTTTTGCCTTGATATTCCCTAGGAATTGTTACTCTAGAATCATTTCCAATATATTTTGTAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAATTTTTCCTAAAATTTAATAAATCTTCTTCTTTGGTAGGATTTTCTGGTTTTGGTTCGGGTTTATCCTCTGGTTTAACTTCAGGAGTTGTGCCAGGTTTTTCACCTTCTGGTTTCTTTGGATCCTCTGGTTTAACTTCAGGAGTTGTGCCAGGTTTTTCACCTTCTGGTTTCTTTGGATCCTCTGGTTTAACTTCTGGTTTGTTTGGCTCCTTGCATCCAGCAGCAACTAATGGCGCTAAAATAACCAAGGGAAGTATCCCCCCCATGGTCAACAAAATTTTTTTTGATTTCTTCATATTCTATATTTCCTTTTTTTGATTTTAAAATAATTTTATTTTTATATATATTAAAAAATAAAACCTCTTAATAAAAAAAAAAAAAAAAAAAAACAGATTTTTAATATAACTGGTTGAAAAAATGGCTTATTTTCTATATTTTTTATGCAATTTATATATTCTTAAGGTCAAAAAAATGCAATAAAAAAACTCAGATTGAACTGAATTTTTTTATTTTTTAAATGGCAATAAAGACAATTAATTGCATTTTTTTAAAACAGATTTCTTACATCATCTTAGTGTTTTAATATATCTGCAAGTTCATCGTTTGAATCTTCATCATTATCCATTGAAAAAAATGATTGTGTTTTAAATGTTCCTTCAGATACAAAGAATTCCTTTGTTTCATCGTTAACATAATTTAAATCGTCTTTTTTTATTACATTGATTACTTCGTCTTGAATTTGATCAATGTCTTTAGGAGCGTCTTTTCTTAATCCCGTGGCGATTACCGAGACAAATAGCTCACCTAATTTGTTGCTTTCTTCAGATTCTAGAGTTGTTATACCAAAAATAATGTTAATTTCATTACCAACAATTTCCCGCATAGCTCCCAGAGCACTTTGAATTTCATTTAAAGTTACCTTTTGAGAAGCACTGAAATTTACTATTGCATCACTAGCCCCAACAACACTTGATTCTAAAATTGGGCTTGTTATTGCAGATGTGATTGCTTTTACAGCCCGATCTTGGCCATTTGCTTGGCCAATGCCGATTACTGTTTCACCTTTATTTTTAATAATGGTTGATAAATCTGCAAAATCAAGATTAATAATGCTTGGGGTGGCAATAACATCAACTATTGTTCTAATAGTTTGCTTTAAGACATTATTTGCGCAAATAAATGCATCTGAAAAAGAAATATTTCCATATTGTTGTAATAGTTTATTGTTTGAAATAAGAATATATGAATCAACACATTTTTTAATTTCTTGTAAGCCTTGCTTTGCAATTCTCATTCTCTTTGGACCTTCAAAGTCAAACGGAGTAGTTACAACTGCAACTACTAAAGCATTGCATTCCTTGGCTATTTGTGCAATCACGGGCGCTGCACCTGTGCCGGTTCCACCGCCCATTCCTGCAGTTATAATTACTAGATCAGCTCCTTGCAATCTACTTTTGATTTCTTCACGCGAAGATTCAGCAGCAGTTTTGCCAATTTCAGGGTTAGCACCCGCGCCAATTCCACGTTCATCACCTAAATGTAAAACGCATTCTTGAGGAAATTTTGCTAATACTTGTTTATCGGTATTGGCAGCAATAATTTGAAAACTATCTAAATGGCTATTTATCATAGTTTCAACACTATTATTTCCACCACCACCTACTCCAATAACAATTATTTTTGCCACCGGGTTATAATTATTTAATTCTTTTGCCATAGAACTCCTAATTTATATCGTTTAGCATTAAATTGTAATACTTTTTGGAATTACTATAAACTTCAATTGTAAATAATTCCCTACTAATTTCATTATTTAAAGCATTTTGCTTGATTTGACATTCTAAAATATTATCTATTGCAAGCATTTCGCAACTTAAATATTCATTTTTGATTTCATATTCAAAGTCACTAAATAAGTTAATTTTTTCATTTTGAATTAATATGCTATTAAAAAGTTTTAATTCGTTGTTGAAATATAATTTGCAAACCTTTGAATCAATTTTATAATTATTAACAAAAGTTGCAACGCTAGTTAATAATTTATTTATAAATTCGCCTAATATATTCTTTATTATTGGAGCAAAATTGCATTTGTAGGTTAAACAATTTACAACTTCACTAAATCAATTGCTTTCTGTACTTAATAAATTGCATTGTAACATTCTTGCTTTTAATTTGTTTAGTCCTAAATCTAATACTTGGCTTACTATATTTGATTTATTAATGTTTAAAATAAATTTAGCTTTATCATCTAATACATCAACAAAAATAGCTACTTTTTGTTTTGAATTTTTAAACAAAACTGAATTTGTCTTTAAACTATCTAAGCAAAATATTTTGGCAATTTTTAAATCGTTGCTACTAAAAATTTCTTGCAATCTTAGACTATTAGCATTTGATATTGTTTTTAAAGAATAAACTTGCTTAATATTAGTTTTTTTAACTAGTTTAAATTCCAATATTTTTTGGCCTATATTATTGAAGCTTGATCTAATATTTTTTAAATTACTAATATTAAAATTGTCGTTATTTAAGGTTAATAGTTTACTATTTATATTTCATTTGCTATCTTCAGACAACATAACAATAACTTCATTTATGCTTGCTGATATTAATTTTTGAATTTCATTCTTTGTTTGCTTTATAAAATTTTTTATATCGCTTTCAGTTAAATAAATATTATTAATTGCTTTTTTATAAATGCAAAAAGATAAAGTATTAGTTTTTTCCATAACTTCTACTTCGATACTATTTTTTAAAAATTTATATGCGGCAATAATAATTTTTTTCATTTAACTAATCCATTAATTTTGTAATAACGCGTAATTTTGCGCTTCTTGATCTTTTGTTGATTTCTTCTTCTTTACTACTTGGAAAAATTACTTTTTGATGTCACTTATGAGTTTCAACAATTAGTATTTTTTTTAACTTAGGGTCAAAATAATTTAAATTTTGATAAAACTTTTTAACTATAACGTCTTCCTTACTATGAAATGTTATAATCGCTATTTTTCCGTCTTTTTTTAATAAATTAGGAAGTTGGCTTAGCATTTCACTTAAGGCATTTAGCTCATCATTCACTGCTATTCTAAAAGCTTGAAAAACTGCTTTAGAAGGATTTTTTTGTCTTACTATTTTAGCTGGCAATGAATCCTTTATTATTTGATTTAACGTAAATGAATCAGATATTGGTCGATTTTTTACAATAGCTTTCGCAATTAAATTACTTAATTTTACATCAGCATTAGATTGCAATATATCTATAATTTCTTTTTCAGTTGCATTATTAATAATTTCTTTTGCAGTTAGAGTATTATCTAAATCCATTCTCATATCTAAATCAGAATTGATTGAATAAGAAAATCCTCTATTTGGATCATCTAATTGAGGACTAGAAACTCCTAAATCTGCTAATATTCCATCGACTTTATCGATATTTAGTTTTTTTAGTTCACAAGTAATGTTTCTAAAATCACTTTTTATTAAAATAAAGTTTTGGCTTATTTTTATTAATTTTTCTCTACTTTCGTTTATAGCTTGAATATCTTTATCAAAACAAATTAATTTTCCAGTTGTTAGTCTTTTTAAAATTTCTTGGCTATGACCACCACGGCCTAATGTTAAATCAACATATATTCCGTCTGGTTTTATTGATAATTGTTCTATTACTTCATTTAATAGAACTGGAATATGTTCCATTTAGAACTTAACTCCTGCTTCAAATAATTTTTGAGCCAAGTCATTTACATTATCATCATTTTCATAGTAACTTTCTTGTTCATCATAAACTTCTTTAGCAAATAATTCACAAGTATCACCAGTTCCTAGGAAAACAACTTCTTTGTCGATAGCGGCTTTATTTAAAAGATGTTTGGGAATCATGATTCTGTTATTTTTATCAGGAGATACAACTTCAGTATTTCCAAAAATGTAGCGAATGAAATCTCTTACGTTTTTGTTAAGTGTGTTTCCTTCTTCCAATTTAGTTTTCATTTTTTGAAACTCAGAAACCGTTCTTAATACTAAGTTTTTATCTATTCCAATTGTGATATAGAATTCTTTGCCTAATTCTTGTAGTAATTTAGGCGGAACAACAACTCTATTTTTATCATCAATTTGGCGATAATATTTACCAAACATTCCCACATCCTCCCACTATCAACTTAATTCTAACATATAAATCAAGTATTTTTTAATTTTTTATAAATAAAAATAAAAAAGAAGGCAAAAAAGCATTAGCCCTCTTGCAATAGAACCATAGTAATTATTTTTTTAATTGTTTTATTAAAAAATAAATAACTGTATAAGTTTTTTGTTAAAATTAAAATATGCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCAAATGCACAAAGTAAGCGCCAAAAATAAAAAATAAAAAATTTATTAAAAAATTAATTTTTTTTAGTTATAATAATTAAGCATTTATTAAGTGCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCATTATGGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCATTGGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCATTTGTTTAAATACACCGCAAAAGGCCAACCGGCCTTTTTATTTTTCATTTAGATTTAAATTAATCGTAATTTTTATATAAAAAACTCGTAACCTTTTTGATTACGAGCTTTTTATATCTAGTTTTTAATCATCAAATTGTGAGTAATATAACCTTGCATATTTTTTATTTAATTCTAGCAATTGCTTATGGGTTCCTTGTTCAATAATACGACCATCATCTACAACTAAGATTAAATCAGCATTTTTAATCGTGCTTAGACGGTGGGCAATAACTATTGATGTTTTACCAACCATGATATTTTCTTTTAATGCTTGTTGAATTATTTTTTCTGTATTAGAATCTACGTTCGATGTTGCTTCATCTAATATTAAGATCTTTTTGTTTCCTAATATTGCTCTAGCTATTGAAAGAAGTTGCTTTTCTCCTTTAGATAATATTGAAGTGCTATTATCAATTATAGTATCATATCCATGTTCTAACTTTTCAATAATATGATGAGCAGATGCTAATTTTGCTGCCCTTATTACATCTTCTTTGGTTGCTTTTGGCTTTCCTACTAAAAGGTTATTTAAAATACTTTCATTAAACATAAAGCTATCTTGTAAAACAACTGCCGTAAAATCTCTTAAACTGTCTTTTGAAATTTCTCTTAGCTCTATTCCATCTATCTTTATAGAACCTTTTTCATAATCATAAAAACGATTCAATAAATTTATAATAGTAGTTTTACCTGCTCCAGTTTCTCCAACTAAAGCAATGGTTTGTCCTGCCTTTGCATTAAATGAAGCATCTGTTAGTTGATATTTAGGCGAATTTTCATTATATCTAAAATATACATGATCAAATGTTATATTGCCAACAGGATTTTCTATCACAATTCCCTTTGAGTGGTTTTTTATATGTGTGTTTGGTAGGCTTAAAAGCGTATTAACCCTAACAGCAGATGCAATACCAATTCTAGCATAAACTAATATAGTAAGCATTGATTGAAGTGTTGATAAAAAACTATATATAAGTGTTAAATAACTTATTAAAAATCCATGTTCTGCATTTGTATTAAAAAGCCCATAAACATGAATATTATTTCCTTTTAATACAAGTGTTACGGTAACTATTAATATAACCAAAATATTGTTAGTAAAAACTATTCAAGGGTTAATTATTTGGTTATTAAGATCCCCAGTAAATGCTGTGTTAAATATGTTTCTTGCTATTTGTTTACCATGATTATTAACTTGTTCTTGTTGTTCAAAAGTTTTAATAATTTTAGAATTAGTTAGCATTTCTTCGACAAAAGCATTTAATTCCCCAAAATGTTTTCTGGTTTGAATAAATGCTTTTTGAGCTTTTTTAGTAATTAATATTCCTATAAATGAAAATAAGAATAGAACAGGTAAAACAACAGCAGATAAAACCAGACTAACCATTGATAAAAATATTACAGATAAAATAACATTTATAGCACATTGGATTAATTCATTTAATAGTTTAAATATAGTTTCAGATAAACTATTAATATCATTTACTAAAATAGAAATAATATTACCTGATTTTTGATTATCAAAATATGAAACGGGAATATATAAAAGGTTTTTCATGGCGGTTTTTCTTATATTCTCGCAAACTGAAAAAGTTATTTTTACAAAGAATACTCCTGAAACATATCTAAAAATTCCATACAATATAAACGATATAACCAAACATGAAGCCATACCTATAAATAATTTTAAATTAAAAGGTGTTTTATGATCCAATACATCTTTTGTTAAACATATAGTAAAAATATATCCAATTAAAAATGAACCTATTGCATAAAAAATTGCATGAAAAAATGAAAACAAAACAGCCAATCAAAATTTTGTTTTTGATTCACCTGAATATTTAAAAACTAATTTAAACAAAGTAATAAATGAAAGTTTTTGCTTTTCTTTAGTTCTTTTTTTCATCATTGCCTCCTTTTCTATTGGCTTAATTGGTTATTATATGATTCATTATAATATGAACATGATTTCATTAATTCTTCATGATTTCCTTTATCCAACATGTTTCCATCAGATAAAACAATAATTTCATCAGCATTTTTTATTAATGGCAATTTTTGGCTAATTATTATAGTTGAACATGAATATTTATTTTTTATATTTTGTAAAACTTGGTGTGCAGTAATATTATCTAAAGCACTAGTTGAGTTATCTAATATTAAAATTTTTGGTTTAGTTAATAAAGTTCTTGCAATTGAAAGACGTTGTTTTTGGCCACCTGAAAGGTTTGTTGCCCCTTGAGTAATGACATGATCTAAATTTTCTTCAAATTTATAAACAAAATCATATGCACAAGCGTCACGTAATGCTTGATTTATTTCTTCATCTGTTGCATCATTTTTAGCAAAAAGCATATTTTCTTTTATTGTTCCTGTATTTAATAGAGCATCTTGGTAAACAATTCCGATTGTTTCATGTAAATTTTTTGTATTAACTTCTTTAATTTCATTATCCCCAATTAAAATCGATCCTTCATTATATAGATAGTTATTAACCAATAAATTCGCAAGTATGCTTTTACCACTTCCAGTAGGGCCAACTAATCCAAGAGTTTTTCCGAACGGTAGGCTAAAACTTATATTGTGTAAAGTATAATTTGGATTTGAAGAATAATATTTAAAGCTTAAATTTTCAACTTTTAAATCGAAGTTTTTTGTTATTTTTTTGCCATTTGATACATAAATTTTATCTTCATTAGTATTTAATACTTCTATAACTCTAGTAGCAGAAATTTTTCCTTTTACAAATGCTGAAATAAATACTGCCCCCAATATAACACCAAATGTTACATAAAATGAATAGTCAATAAAAATATTAAATCTTACAATTGTATCATATGTTGCTACATTAGAAATAACATCATGAACAACTAATGAATAAAGCACCACAACAAATAAATTAAATATTCCAAAGAATAAAGGAGCAATACCTACAAAAATGCTACCAGCATTAACATTAAAATTCTTTCATGTTTTATTTATTTTTGCAAACTTTTCTTCTCTATTTTTATATAAATCATATATTTTTATTAAACGAGATCCTAATATATTTTCATCAACTTGCTTAGTTATTTCTTCAACGGCTAATTGAGCTTTTTTAATTGAAGGACTTGCTTTTTTGGCCATAATAGAAACTATTGTTAAAATTATCGGAACTAACAAAATTAATCCTATTGCCATTTTTACGTTATTCATGACGGCTAAAATACATCCACCAATTATCATTGAAATAGCCCTTGTTAAGACATTTGTAGCGCTTATTAAAAATTCTCAGAAAACCGCAATATCATCACTCACCCTAGTAATTAATGATTCAGGTTTCAAATCAGATATATTTTTTAAACTTAATGTATTTAATTTTCTAATAAAGTTTTCTCTAATATATCTACTACTTCTTTCTGCTGCATATACAATTAAGCTAGTGCAGCCAAATGTTAAAGACATTGAAATTATAGTTAAAACTATTATAGAATAAATCATATAATTACGAGCATTAATAGCATTATTAAATGTAAAGTTTATTAATCCATTAAAAAAACTTAAATCTACTTGGTTATTTTTATTTTCAATAAAAAGCAATCTAATGAATTGAGAAATCAATGTAGGAGTAATCAATTCAAATATAACTGAAATTAAAGAAATAAAACTTCCTACAATAAACAATATTTTAATTTTTCATGGTAAAAATTTAATAAGCTTCAACATAATTTCTCCTTCTTTTAAATGTTTTTTATTGCCACTTTCAAGGCTAATTAATAAATTATAATGAAACATAAAAAAAAGTAATAATTTAATGCAAAAAAATACGAAAAAAAAGCGATTTTTCGCCTTTTTTTGTTTAATTTATTTTTATAAACTAATTTTATTTATAAATTCAACTTCTATTTCATTTCATTTAGCAGTGATTGTATATTTTATATATTCTTTACCTTTGTTTGTATATTTTTTAACTGAGATTTTTTCAAATGTTAATCCTTCTGGTAATTTGTATTCGAATTTAGAAATATCATCTTTAATTGCTTCATCAACTGTTTTTTTATCGCCAATGTATTTTACAGTTATTAATTCTCTAAAATGTTCTTTTAAAACTATTTTTTGCATAAAGTTTTTATCTTCATTTACCACTTCATTAACATTGACACGTTTAGATGACATATTGCTAAAATCATTATTTTGAAAATCTTGAATAGAAACTACAGAAACTCTAATTGATTTTACTTTGTTATTTTCTATTTTAGGAAATGAAAAGATATAAACATTTTGTACATTAACCATACTATTTGTATATTGAAACGCAAACATTGAATGTTTCAATTTTAATGTTGTTTTATCATAATCAAAATTTTGAACCGAAATGTAATTATCACCTTTTTCAATTATTTCTTTTTCAATGTCTTTGCTTAAAAGACTAATATTTTTCAATTTTTTTATAAACATAAATGTTGAATTATTTTTAACTTCATCTTTAGATAATTTATCTTTGTTTTCTTTGAAATATTTCAAGAATGCTTCTTTGTTTAATTCTTTTACGTCTTGGTCTTCAATAGTTGATGTTGGGGCTTGTTGGTCATCTATTTCAGTTTCATTATTTTGTTTTCTAATCTTGTTATATTCATCAACACCCAATTTACTTAAATCAATTTCAACAAATTTTGATTTCTTAGTACTATTTTCATAATCTTTAAATGAAACAGTAAGATTAATCAAACACTTATCACCTTCAAATTTTAATTCACCTAATGTGTAATTAATTTCACTGTTTTTATTATTAATTCAGTATGATTTATTTTCTAATTTGTCAATAGATTTCTTTGAAATGTAATATTTATCTTTAGTATAAAATGGATAAATTGAAGTTTCTAAAGCTTTGTTTAAAAGTTCATCTTGTTTTTCTGGTGATAGCAATGCAATATTATCTAAACCATTTGCAAATTTATCTAATTTTACTTCAATAGTTTTAGATTCACTTATTTCTTTATTTAAAAGACTTGACAAGGTTAAAGTCAATGTTATTTTTTTATCTTCTTCAACTTTTAAATTGCTTAATACTACTGAATATGAAGCTTGATCATAACCTTGAACTGCAAAATTTGGTTTTTTATCAAAATTATTTATTTGTTTATTTTTTAAAATATCTTGATATGACTTGGCATTTGCAACTAAAACATTTGTTTTTTCAATTTGATCTTTTAGTTCTTTTCTTGCCTTATCTATTTTTTCTTGCAATTCTGGAGTTAATTTAAAACCTTTGATTTCATAAATATATTTTTGACTTGAGATTTGTTTATTCTTGTATGATAAATAGTAAAGAATTGTCAAGTCATATGTTCCATCTTCTCCTGTATTTTTAGTAATTTTTAACGAATCTTTAACAATTGAAAAGTTTTCTTTAATAGTTTTTAAAAATACTTTTGATGTGTCTGCGTCTTTTATGTATGTTTCTTGTTTATTAGGATAGTCAAAGACTAGATTTTTTGAAACTTCTTTAAAATCTTCTTCTGAAACTTGATCTAATCCAGAATCTAAGTTTCCTAAATTACTTTCATAAACTTTGTTTGAAATAATATGTTTACCATTTTGTTTAAAAATTGCAACTTTATATTTCAAAATAAAGTTTCCTTTTTCATCTTTTGTTAATAACAATTTAGAGTTTAACTTGTCTCCACTTCTAAGAGGTTTTTCAGGATTTACCAATTGAACAGATGTGCCTGATTTAGAATTTCATTTAGCAATAGCAAATTCTTGATTTTTAAACTTATATTCTCCTTCAAAAGCCATGATATTTTTGTTATTTCTTTCAAAAAGAATTTTGTATTTGTCTAGATTATTTAAAACCTTTTTAATAAATTCTAGATTTTCAGCATTTCATGTTATTTTTGAAATTGATTCTTCGCCATATTTATCAGCATCATTTTTATTTGTTTCTTCTTCATGACTTGGATCTTCCGGTTTAACTTCAGGTGTTGTTCCTGGTTTATCTTCTGGTTTATCTTCTGGTTTAACTTCAGGTGTTGTTCCTGGTTTATCTTCTGGTTTAACTTCTGGTTTTTTTGTTTTTACGCAACCTGCTGCCACTAATGGTATTGCAAAAACCGAGGTTGCAACACTACCTAAGGCTAATAAAATTTTTGTATGTTTTTTCATTTTTTATATTTCTTTTCCCTTTTTCTTAAATTAATAATTTAAAAATTTATCACACGAATAAAGCCTCAAGTTCAAATGACTGGTTTTTTCTTTAAAAAAGAAAAATAAATAACCGTTTGGTTATTTTTTATTTAAAGATAATGTTTTTAAAAGAAACAAAAAAATAATAAATTACAAAAAATGTTACGTTATTTTTTGATTTAGCAAATATTATAATGTGTTTTTTTGGCCTATAAAAATATTTAAAAAACTAGCATTTTAAGCTAGTTTTTTAATATGGTACATCCTGTGGGGCTCGAACCCACGACCCAGGGATTAAGAGTCCCTTGCTCTGCCAACTGAGCTAAGGATGCGTGTTTTTGCGTTATTTATTATAACATAACTAAAACATTTTTTTAAAATTAAACAAATAAAAAGTAGCGTTTGCTACTTTTTTGTTACAACTTGATGATATGGTACGCGAGGAGGGACTTGAACCCTCACGCCGCAAAGCATTAGTGCCTAAAACTAACGTGTCTGCCAATTTCACCACTCGCGCATATGGTGCCGTTTATAGGACTTGAACCTACGACCTACTGATTACAAGTCAGTTGCTCTGCCAACTGAGCTAAAACGGCTTTTGTAAATGCTTTATTATTATATAAGGAAATTTTAAAAATTTTTACATTTTTATTCAAATTTAAAATTTTAATTATTCATAATATCTTAAAATAAATGTTGAAATATTATTTTTTACTATTACTATATGATTTTTTTTATTAACTTTAATTTTTAAAGATAATAAATTAATATTTTTAATGTATGATTACATGGCAAGGACAAAATTATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAGTTGGACATTGTGGAACATTAGATCCCGAGGCTGAAGGTCTTTTAATCGTAGCAACAGATGAAGATACAAAAGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCAAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTGCCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATCGAATTTAATAAAGTTAATCAATCCATCATCATTAAGGCCAATGTTTCAAAAGGCACATATATTAGATCATTGATTCATGATTTAGCTAATATATGTAACACAGATGCAGTAGTAGTTGTTTTAAAGCGAATAAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTCAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATAACATAGTAGGATGAGGAAAATCAGAATTTGGAAATGTAACTATTTTCAAGCGATTTTTTAAACGCCTTACAATGTTAATTTCTAGCTAGGAGTAAACATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTGTTGCCGTTCGGAGTTACTCAATTAACACCAAGAATGCAAGAAATGTTTGAGAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATTGATAACCCTTATGTTGATTATTTTATAGGTGCAAATGGAACTTTTATTCTTGATTTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTGTTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGATTTTACAGACCATATAAAGACAAATTTATTCCAGAAAAAGAATTCGAATCGCAACATGATAAAAATTATTTAATTACTATTTCAACTCCAACACCATCTATAGTAGCAGCTGATTTAGAAAAATTCTTCAAAGAAAAAAATCTTGATTTATGAATTCTAGCTCAATGAAATGGTGGCATATTCATCTCTGCTAAAGGAATAACTAAGGCTGTAGGTTTAAAAATACTAGGAGATTTATTAAATATTTCTCTTGATGAAATGGTAGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTAGGATTAGGTATTGCTATGGGCAATGGAGAAGATGTTACTAAAGATGCTGCAAATGAAATTTGCTTGCCAGTTACTGAAGATGGTCCATATTGAAAATTAAGAGATAAAGGATTTTTTGACTAATAAATTTCAACCTTATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTCTAAAATATAAAAAATTACTTACTCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGTTATCATTTAGTAAAAGAAGCAATCAAGGCAAATATAGCTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAAACATTATCAACAACGCAAACACCTCAAAATATCATTGCAATTTGTAAAATACAAAATTTAAATATTAAAGCAAATAAAATAATAGCCCTTAATAAATTACAAGATCCCGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGCTTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATGACATTGATTTTAGAAATAAAAAAATTATTGTTGTTTTGGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTATATTCCTATTGAATTTGAAAGCCTCAATGTTGCTTGCTGCGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAATGCAAGTTTTTGATTGAGATTTTAAAGCAAAATTAAACATAAACGAACCTTTAACAATTTGTTTAGGTTCGTTTGAAACATTACATTTAGGGCACAATGAATTATTTAAAATAGCAAAAGATTTAAAGCAAAGTTATCCAGATCAAAAATTAGCAATAATGATGTTTAAAAATCCAGTTAAAAATTCTCGGGTATTAGCCAAAAAGGCAATGCAACCAAAAACCAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGCGATTTTATTCAAGGATTAAAAACAAACAACGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGATTTGGTTTCAATAGAGAGGGAAACATTGATAGATTAAAAAAATATTTCAATGTATATGTTGCTAATGAATTAAAGGTAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAGTGGCTTGAATAAATTACTAATAGACAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTCAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGAAATAACATTGAATATCACGGCTTAATTTTTATAAATAAAAATCAATTTGACTCAAATAATAGCCTACAAGAAAGCAATATAATTTATTTATTTGATATTGATTATTTTATTTCGAAATATCAAGAACTTTACATTGAATTTTTGCAATGCATTAGACATATAAATAATAATAGTGAAAATCAAATTTCAACAAATGATATTGAAATTGCAAAGAATTACTTCATTGCCAACAAAAAATAATATTAAACAAAAATAGCAAGTTTGTAACACTTGCTATTTTTTAACAAAATATATTATTTTAATTTTAATCTTTGTTGTTCAAGTCAATATATTCAATATCACCGTTTGAAATAACTTTAATTAACCCTAAATTATTTAAATATCCATATAGATCGTTTAATTTTGAAATATTATTTTCTGTTCTTGCTTGTAGTAAGATTTTTAAAATTGTATTAGTTAAATTATTTTTTAAAATAGAACTTTTTGAATTGTTTAAAACTTGTTTTATTAAATCAAAGAATTCCAATGAACTTATTTCTGATATTTTTCTATTGTCGCAATCTTTTGGCATTCTAAAAGTATATGGCTTTGAAACATCATGATAAAAGATTTCACCATCAGGATTTATTAATTTTTCTAACATTTTATTGTCAACAAAATAATTTATAGAATTTTCACAAAGGTATTTATAAGTATATTCGTTTTTATTTAAAGGTAGGTTCATGAATATATCTTTTATCATTTTATTGATTTCTTTTTCTCTATAAGTATGGCCATAATTAAACATTTGTTTAACAATTTCATAGCCATTTAACAAAGGATTTCTTTTGTTTGACTTTTCTATAAAACATGATATCTCACGATATACATAACTAAAAGGAAGCCATTTTTCAAAATTAAAATTATCTCTTTTTTCAATAACTTCAAATTGACTATTTTCAATTGTTTTATATTCATTTTTCTTTACAACAAATTTTTTTTGTTTAATTTGATTTACTTTATCAACTATTTTTTCTAATTCTCTTTTTTTGTTTTTAAATCAATTTAAAGATCAAATTCTGTGAATTATTCAACCTCTTGACTCAAGTAGCATTTGTCTAATCGCATCTCTATCACGAGCGCTTTTTGATGAATGATATGTGGCGCCATCGCATTCAATACCTAAAATATATTTCGATGGATTTTTATCATCAACAATTGCTAAATCTATTTTAAATTTCGAACATCCTACTTGAGTATTTACTTTATATCCAAGCTTCTTAAGTTCATCGTAAACTTGGATTTCAAACTCACTATCAAATGTTTTAGTGTCATCTTGTAATATATCATCTGATTTGCTGTCGTAACCAAATTCTGCAAATTGTAAATATTGCTTTAAAAATCTTAGTCCTTCATTTTTTAAATCGTCTACTTTTAAATCAGAATATGATATTGAAGATACAACAATAGTTGCTTCCTTGGCTCTACTTATTGCGACATTTAGTCTCTTTTCACCGCCAACATTATTTAATGGTCCAAAGAATCTTGACAATCTTCCGTTTTTATCCCAACTATTTGTAATTCCTAAAATAATGATGTCTCTTTCATCACCTTGAACACTTTCAATGTTCTTAACAAAAATTTTATTTTCTGTGTCTAAATCCATATATTCAGGATTTTCATTCATAAATAAGAATAATTTGTGATCAATTAAGTTAGCTTGTTTTAAATTAAACGTTACAACCCCAAATGTTTTTGTATTGCCATAATAATCTTTTAATTGCTTTATTAATTCAATGATTCTATTTGCTTCAATTTCGTTTGTTTGATCTTCATAAATTCCATTGACCTTAATAAATGAAACGCCTTCAAAAGGAAGCAATTGCCCGTCTTTCTTGGCAGTTTTTTGATATGTTGGAAAAGTAATTAATTTATTATCATAAACAAACTTATTTGAAGGCAAAATTAATTCTTCAAATTTTGAGCGATAGTGTCATTTTAATGTAACTTGTCTAAATGAACTTTGCATTCTTTCAAGAAGTGAATCATAATCATTTTCTTCATTGACTATTTCTTCATCTTCGTTTGGTTCTTGCTCGCTAGACATAGTTTTAAAGAAATTAGTTGGTGGCAATTGATTTATATCACCGGCAATAATAATTTGTTTAGCCTTAATCATAGATATCAAGGCATTTTCTGTCGTTAATTGAGAAGCCTCATCAAAAATTACACTATCAAATGTAATGCTACTATCCGCTAAATATGTACTTAGCGAAGAAGGAGACATCATAAAACAAGGCTTAATATTTTGAATTAAATTTGGAATTTCTTTAAATAGTTTTCTAATCGGTTTTTTATTTCTAACTTTTGAATATTCCTTCTTTAAAATTTCTAATTCATCATTGTATATTGTCGAATTTAACGTAGGCAAATTGTTTGATAAATTTTCAATTATTTTGAATTTAGCAATTTCATTTAGCTTTAAACAAGAGTTTTTAAAAACATTGATAATTTTTTCATATTCTCTATCGTTTCAATCTGCTAAATCAATTGATAAATAGTAATCAATTAAAAGTTGATAAAATCTTTTATTAAAAATATTTTCAATACTATTTTGTTTTAAACAATCAATATCTAGCAATTCAATAGCATCCACAAAATCATTAGTAAAGGGATTGTTTTTTAATTCTTGTCTTGATTTTATATAACTACAATATGCTTCAGGATATTCATCAGAATCAATTAAAAAATTTAGTTTTTTTATAAAATCATTAACTTTATAATTTGAATTTGAATAATCTACATATTTATTATTAAAGTAGTCATTGAATTTATCTTCAAAAGTCATTAAATTTTTATATGAATTTATGTAATTATTTGTTAATTGAATATGGCTTAATAAAAAGTTAAAAAAGCTAATTTTTAAATCTTGGTCAAATTTTAATTCACTTAATTGATTATTAATAATTTCGTATTTAGAAAATATATTTAGTTGTTGCTTTAAACTATTAAGATCCATTTCATTATAGTTTTCTTTAAATGAAAAAGGAATTTTATTTGTTAAAAGCGTAATTGCATTTTTAGCAAGTTGAATGTTATTTAATGCTACTAAATCTTTTAACAAGTTTTTGTAATTTAAATTAGAAGATATAAAATTAATTTTTAATTCTTTTTTTATTGCTCTATATCTCTTTGAAAAAATACGCTTAAAAATATTATTTCGAGATTTCACAAAATCTAAATTCTTATAAATTTCACTATTAAAAATATTTTGATTATATCTTGAAAGAATTCAGTCAATATCAGGTTGAATTCTTTCTTTTTGCAATAGTATTTCATTTAGGGTTTGTGTTGCATTAGTTTTGTTATACGAAAAAATTTCTAAATATTTTGGATTTATATCTATATCTTGTATATTTGAAAAAATTTTAAAAAATGAATTAAATAATGAATAATTGCTATTTTTAATATATGGTTGATAATTGTTTGGAATTTCAAGATCTAAGAAATTGATTAGGCTTTGAATTTCATTTATTAAATGGCTAGAAATCTCTTTAATGTTTTCTAAATCTTGAAGCTTTACATCCAAAATCGAAAGCCCGTATCATAAACTTTCCTTAGCGTTAAAATTTATATTTTTATAGTTTAAATTAAACTTATTTAAACTACTTTGAATTTCAACAAACTGTTCATTATCAATTTTTTCAAAATCTTTAATATCAAAATTTATACCTTTTGTATTGGCAAGCAATTTTTGATAATCACCAATCATTAAATATAATGAGCGACCTAGTGGTTTTCTATTTGATAATATCTTTCTAACAAAATCATTCATTTTTTCTAATGAATCAGTATATAAATAATCATTATTAGCAATAACTTCGTCACTTACTTTTTTAAATTTTTGACCGTCTTGCAATGTTTCATACAAATCATTAGAAAAATCTTTTTTATTGCTGTTATTGCTATGAATAAGCAAAATAAAATTAGCAAATCCTAAATTTTGGATTGATTTATAAACAACATCCAAAGCAGCCTTTTTTTCAGCAACAAATAGAACCTTTTTATTTCTTGCAATTAATTCACTTATTATATTTGCTATAGTTTGAGATTTTCCAGTTCCTGGAGGACCTTGAATAATAAAACTGTTTCCTTTAATTGCTGATTGAATAGCTGCTTCTTGCGAAGAATTAGTCATTAGGCAGTGATAATAGCCTTTAGGAGAAACTATATTATCAACTTCTTCTTCAGAATACAAATATTCAGAACTAAAAACATTGTTAATCCCTGTCATTGCATTCATAATTTCTGAATTAATGATATTATCTTTGTTTTTATCAACATCTAAATAAATGTCCATAGAAGAGAACCTAAAAGCAGACAATGAAGCTTCATCAACTATTTTTCAATTTTGTTCTATAGAATCATTGAAGGAATTTATTTTTTGATTTATAAACTCAATGTATTGTGAATATCTATCATATAGAGAACCTTCAACATTATCAAATGCTTCAAATTTAATATCAAGTCCAAAATCATCATTTAATTTCTTTATCAATGAATTATTTACAACAAAGTCATTGCTTTGAATAATTATTTTTTTATTTTGCAATCCATTTTCACTTTCAATTGAAACAGGCAAAAACAAAAGTGGGCTATAAACGTTTTTTTCTGTTTTATCCTTTCATTGAAGCCCGCCAAAAGCCAAATATAGAATATTAATTCCTTGTTCTTCTAATAAAGTCTTTTGCTTTCTTTGCAATAACGACAATATTGCTAATAATTCTTTTTGAGAAACAGAGGACAAAAATTCATTGCTTTCAACTTCAATGTCATCATCAGAATCTTGAGAACCAATAAATTTAAATTCACTTCTATTTTCAATTTTATCTAAAATTGATTTTATGTCTTCCTTTTCAATTGTTATAGTTGATGATTTATTTTTTTTATAATTTATCAATATATTTCTTAATGTTGTGTCTAATAGCTTATTCTTTAAAGACTCATATGCCAAAGTTTTTGTAATTAATTCTTTATTTTCCATTTCTAAATTATATTATTTTAATTTTAAAATAAATGTTGTTTAATATAAGATTTTATTAGCATATTTTAAAAAACAAAAATTTTAACGCTTGATGAATTAAAAATTGAAATTTTTACATTTATTGATCGGTATAATAATAAATCCATTAAAAAAAATGATTCAAAACATCCCAACAATGTGGGATGTTTGTAAAAAAACAAGACTCTTTTTATTTTGATGTGAGTCTTGTTTTTTTTACTTCTAGTTGCTTTTTTCTATTATTGAACCCATTTCTAAAAAACCTTGAGCATCTAATGCGGCTCCGCCAACTAAAAAACCATCCAAATCCTTAATTTCAAGCAGTTTTTCAATATTTTTTTGTGAAACTGAACCACCATACAACACCGGAACTTGATTATTAGTAATTTTTTTAATTACAGATATTGCTTTTTCGATGTGGTTATTTTGAGGAATTTTTCCATTTCCAATGCAATAAGTAGGTTCGTATGAAATAATTAAACGATTATAAACATCAACATCTTCTAAAGCCTTTAATATTTGGTTTTTTATAATATCCTCAAAGACTTTATCGCTACAATTTTCTTGATCTTCCCCTATACACAAAATAGGAATAATGCTAGTTTTTTCAATTTGTTTAATTTTTAAATTAATCAATTGATCAGTTTCATTAAATAATTTTCTACGTTCACTATGTCCTATTAAAACAAAGTCAATGTTCAATTCAACTGCTGATCTAAATGAAACTTCCCCAGTGTATGCACCTTTTTCTTTATAAAAAATATTTTGAACTCCAAACAAGAAATTTCTTTTATTATATTGTAAAGACAGAGACATTGCATCGAATGACAAAGCCGCTCCAACTATTACATTTTTAAAATTCTTAATTTTATTTAATGATTGTAGTTCATTTAAATAATTCTGACTTTCTAAATAAGATAGATTCATTTTTAAATTACCAATTAAATATTTCATATTAACCTCTTTTATTATCAAACTATTTTTAATGCTAAAAATGGAATCAATATAATTAATATAATTAATCCATATAAAATTAATAAAAGAAATGATAAATTATTAATTGATTGATAGTTTCTTTGCTTTTCAGCTTTGAGTCTTTCACGTTCAAATTCTTGGCGTTCTTTAGCAATAATAATATCCTTTTGATGAATTGTCATTCCTGAATAAAATTTGTCTTTTGCGCGACGCATTATGATTCTATTTCTAATTAAATTAGCAATAGGATTGACAATTCATTTTCCCATTCCTTTTCTATATACAACAATTATAAAATTTATAGTAATTGCAAAAATCATTGATACAGTCAATGAATTTTGCCAAGAATGAACATATTGACCATCAACAGATTTTTTTGAATATCTAACTATAAAAATTAATGCAATATAAATCACTAATGAAACAAAAAAATAACTAATCGTTGCTTTATTTCAAGCATTCTTTCAATATGATATTGATTTCTTTTGATTTCTAAAATTTTTTTGTTTTAATGTCATAACATTATTTTATATTATTTTTTAACAATGCAAAACAAATTTAAAATATATCTGATTAATGATTTAAAAAATAAAAATTAAATATTATTTTGTTTCTTTGCATACAAGGTAAATTAATTTATTTGATTAGTGAATTTATTTTTTTGTGATAAATTTCGTTAAATTTTTGTTTATCAAGCTCAACTTGTTCGAAATTTGTATATGAACTGTCTTTTTCGTTATTAACTAATTTATTAATATATGGGTTTATAAATTTTTCAATAAAATCTTGGCCATCTGGTTTTTGAAATATTTTATTTGAATTAATTTCTGCATCAGTCAAAGTTTCTACAAACTCTCAAATAGACTCGTTTAATGCTAACTTTTGACTTATAGAATTAATTTGTTCATTTAAAGATTCTATTAAATTATCATTTGCATGACATTTGTTAATTTTTTCAATAATTTGTCCCTTAAAATATTGATATTGATTTATTAATGGTACATATTTTTCTTTTAAATTGTTTTTTGCAATTTTATCGATAATATTTTCAATTACTTGGTCTTGGAATTTTTGTTGCAATGCTAAATTAATAAATTTAGTTTTTTGAATTCTAAGCGAGGTTATTTGCTTAATCTCGTTCAACTTATTTAAATAACTAGCAATATCTTTGCTATTAATATTGTTCAATAATGCTTCTAGATTAATTGCCTTTAAAGTTAAAGATAAATTATCAGCAATTGATTTTAATTCGTTTCTTATTAATTTATCTATTTTAGAATTGTCTGCATCTAATTTAATTTTATTTTCAAGATTATTTAATTCGTTTACTAAATTATCAATTTTTGTTAAGTCTTTTAAAAGTGGTTGAATTTTAAGTATTTTTTGTTTAGCAACGTTTATTTTTTGTATTTTATCGTTTAAACTTTCTATTGATGATTCAGGGGATTCTTTATTAAAAGATTCCAATAATTTCTTGGCATCCTGAACTTCTTTTAAGGTGTTAAATTGTTCAGCTCCTTCTACCAAAAAGTTATCTTTTAAATAACTAAAGAGATCAGTTTTTGCATCATTAAATTTTTTTAATAATTCTTCAGATTTTTGTTCATTTTCATCTTTAGTTTGTGTGGTTTCTATATTTTGTTTAGGTTGCTCAGGTTTTTTAAAACAAGAAGCTGCAACTAATGGGGTTATTAAAATAGTTGAAGATAACGGCAATATATAAAGTAATTTTTTTCTCATTTTTCTCCTATAAGGTGTGCAATATTTTTTTAATTTTATATTCTTAAATTGTACTATTTGCTTGTATTTGTTATAAATCACGATTATTTTACCAAAATGTTCAATAAATTTAAGTAGTTTTATCAAAACAAATTATTTTCATAAACCTAAATAATTAAAAAAATAGTCTAATTTAGGCTATTTCTTCAATTAAAAATTTTTCACTATTTTATATTAATCAATTTCAAGTGGTACTTCGCTATCACCATCACTACTAAAAATTCCATTATTTGATTTATCAGGGCATATTACACTATAAGAAATTACTGGCAAATCTCTTAATCTTCCCTTTGATTTTAATTGTATTGAAATGGGGTGGCCAAACGGAGTAAGTATCTTAGGCAATGTAACCTTAAAATATTTTACAAAATCTGCATCATTCATTACATTTTTATATGCTTTATAAACTTGCGAATTTATTCCACTAGTTTGTTTATTTTTTATGCTGTTTTCAATTTGTTCATCAATAGATTTATACTTATTGATTATTCCTTCTAAAATTCTTTGTAATTCTTTTGTTCCACGTGCAGGGTCATTTAATATTTCTTTACCTTCATTAGTTAGAGTGAAACCAAATTGGTCTCTAATATATGACAATTGGCTTTGACCTAATTGACCTTTTGCTCTCTTATTAGGATCTATAATGTCGCCAGATTGAGATTGTCCAGTCGAATCTTCACCTGTGGTTGTAGAATCTTGAGATTGTCCAGTCGAATCTTCACCTGTGGTTGTAGAATTTTGACTATCATTTGTTGTTTTGCATCCTGCTGCAACTAATGGCATTGCAACAACTGAAGTTGCAACGGCACTTAGTATTAATAAGCGAATTTTATTTCTCATATTATTCCCCCTGAATATAAACATATCGTTCATCTTCAATAACTTGTAAAAATTGGTTACTTCTTAATTTACATATTATACTATATTTTATAAATATAGTATCTGATTTTAAATTTGTATCACCATATCCAAGTTCTAATTTTAATGTATGATTTAATAAGTATTTCGAATCAGGCAATGAAATTTTAAAATATTTTAAAAATAACTCATCACTGAATATGTTTGACTTTTGATCATCTTTTTTGTATTTATCATTGAGTTTATCAATGATTTTGAATAAAAATTTCTTATTCTCTTGATTAAGCGGTATTTTGGATTCAAAATTAAAACTATCATAAATTGCTGCAAGCATAGTACTAGACATGTTTTGATTATTATTAGATAAAACATTATATTTAGTATGACATGGGCAATTAATTCCTTCAATAATCTTGGTTTTTAATAAATCATTTTTGGCTTTTAAATTAGAGGGTTTTTGGCATGAAACTGCTGCAATTGTTGGAATTATTAAAGCTATTGGTAAACAAAATAGTGTAATCTTTTTATTCATTATTCTAAATCTAATTTTATTTCTTGATCTTTGGCATCATATGTTTTTACATCTTTGCAATATATGTCATAGATTAATGAAATATTGTTATTATTATCAATTTTAAAATTAATTATCAATTGGTGATTTACACCTAAATTCGGCTTAACCACTTTAAACAATTTTAAAAAGTTTGCATTATTAATTAACCTCAAACCTGCTAATTTATTATTTTCAGAAGGGCTAAGATTAATTTGCAATTCATAATCTTTACAAATCTTTTTTAGCTCATCTGCCAACGCCTTATTGCCATAATTATTTTTTAGCATTTTGCTCTGTTCAGTTAATTCGAAAACAAAATCATTTTTAATTGCTTCTAATTGAGAAGAATTAAAAATTCCACTTTTATTAGTGTAAATTTCATTTGCTTTTGCTGTATTTTCATCTGTTGAAACACAAGATGCTGAAATTATTGGCGAAGCTAAAACTAGAACTGCCCCTATAGATAATATTATTTTTTTCATATGTTTTTCCTATTCTATTACAATATCTTGCAAGTAAGACAACCCGTCATAACTAATTGGTTTATTAATAAATTTTTGGTATATTACTTTTGAAAAACCAACAGTTTTAATGATATTTTCAAATGAAAAGGTATAAGAAAGTAAACTGTTAATATCATTAATTATATTTAATTGGTCTTGGTAAGAGATATTTTCTAAGTATTTTAATATAATTGATTTTATTATAAAGAAATTTTTGTTGTAATCTTCTAAATTTTTGTTAAAAATTTCATTATTTGTAATTAGTTTTGTTTTATTTAATTCATTTAATATTTTTTTGGTATTTTCTAGAATAAAACTATTGCTTTTAGTTAAGCTAGAAATTTTATTAAAGATTAAAAACAATTTATAGTTTTTAGCAATGGCTGTTGCTATAAATTCTTTAGTATTTGATTTTAAAAGTTCAAAGCTATTATTGCAATATATTGAAGCATCTTGATTAAAATACAAGTCATATTTTGCTAAATCATCAATAACTACAAAATTTGGCTTTAAATGTTTATTTACTGTTTTAAATATTGTTTTGATATGTTCTAAAACATTTTGCCTTTGGTTATTAAATTGCAAATTCAAAAAATTTAGTGGAATATCAAATTCAATATCAGCAGCATCTGGATATTGTTCATAAAACTTTTTTATTATATCATTTAGTTGTTTAGATATTTCTTGAAAATAAGCTGATTTTAATTTGTAATCAAGATTGCTTGATTGAAGATCGTTTAATATTTTTTTGTTTGCAAGATAATAACTATTGTCAATAAAGTCAAGACTATTTTCATGAACACTTTTTGGAATCAATGGCTTAAAAATGTTTACCATAGAATCTTTTAAAACATGATAATTAATTCCACTATCAAAGCTTGAAATATCTAAATCGCTTGGCGCTTGAGATGTTCAAATATCACCTTGGTTAGCCAACATCCCATATTGATTAATCAATTGGTGAATCAATAATAAAAAGGCAATATTTTTGGATTTTAATATTTTATTTATATTCGTATTTTTATTTAATTGGTTTGCTTTTAAACCATATGCTAGCATTGAAAAACTATCATTAAAAAATTTTTTATTTGCAGTCGGCATTCAATTAATGGTAATATTATTACTCAACTTATTTTTAGAAACATTACGAGTATAGCTTATATTAAAATCATTATATTTATGCAAAATTTCTTGTTGTTGATTTATGTCAAGATCGTCAAAATTCAATGTAGTTACAATATTTTGCAAGAATGCATGATACATTTGCATACCAAAAGTATTTTTTGATATCGGCAATGTATTAAATTGAAAATCAATTTTTGAAATTGATTTTTTATTTTTATCGTAAAAGAAATTCTTTAATAATTTTATTTCATCTACATTTACTTCATTAATAAAATAATAAGATGCATAATATCTATCTTTAAAAGTTTTATCATATTCATAAAATCAGTCAAGGTTTGTTAAAAAATCTTTATATTTTTCATTAATGATTTCATAAGGCATTGCATCAATGTAGTTTGAATGTATAAACAAATTGTTAAATAAATCAGCTCAATCATGTTCAATATTATCAGCACTACAAATACTTAAATTTTTGTCATTAAAGTCTAGTAATTTATAAACATCAACGTTGTAACTTTTTAAATATTCAAACAAATCAAAATTATTCAATTGATTTTGCTTTAAAAAATCTTGTTCTTTATCTTTATTAAGTGTTATATTATTTTTTGATGCTCATGATCTTCTAAAATTTTTATTTAAAAGTAGCATTCTTAAAAGACCTAATTTGAAATCTTTTACATTTACTCAATGAACTTTATTGTTTATATTTCTAATTCCTTGCCAATTAATTCAATAATTTTTTCTTTGATTTATACTGATTGAAATATTTTTTGCTTTTCTTAGAGATTCAATGAATTGTTTGCTATTTATAGACATTGAATTTTTTGAAGGAACGAACAAATATGGATTATTAAAACCATTGCCCTTGTCTTTGCTGGGGTAATATTTGTTGGGATCTTGCTTATTCAATTCAGTGTTTTGATAGTCAACATTTAGTTTATCATCGCTATTGAAAAGTTTATCATTTATTTGAATACTATTACCATATTCAAATTTATAACTTAATTCAGTCGGGCGCAAAACTATATTATCCCTAAAGTCTATTTTAGGTTGATTTTTTGAATAAATTCTAAGAACTTTGGCTCCTGTCATTAAATTTATTTCTTTTAAATTTTGGTTTTTGAAATTTGGTATTGAACTGCCATATTCTAAATAAGGATTTGCAATATTAAGCTTTTGAATATATTTTTTCTTATCTTCATTAAAATTATTAAATTTACAAGATATTGATATCGCAGATGTCATTGTAAATAATGCTAACATTGAACTTAAAATAAGAATGTTTTTTTTCATTTTTACCACCTTAAAATGCCGTTGCTGTATGAGTGTGAAAACATAAAATAACTATAGTTAATACTAGTCCTAAACTAAATAAAGCAAATGAAGTATATAAAACTTTCGGAGATTGATTTCGAACATGGATAAATTCCGGAACCAATTCAAATATGGACATAAATGTAATAATTCCTCCACCTAATGCCAAAAACGAAGGGATTATTCAAAACACTTTACTATGTTCAATATATTGACCAAGCAATCCGCCAATTAACATAAATGGGATAATTAATAAAAATGTTAAGAAGTTGTATAGTATGGCTTTTGATTTCTTTTCTCCATATTGAATTTGACGATAATAAACAACTAAAATTTCAAGTAATATATGAATATTAAATGTTATTATTAAAGGAATATTTGCTTTATATGTTCCACCAATTGATGTTAATGTATAAACATTATATCCCAAAAATAGTCCATCAATAATTCTATGACTCATTAGCATTAAAATAGCGCCTCATGCTGCTCTTGGATTGTCGAAATCTTTTATTGAAAATAAGTGGTCCGAGTGATTGTGTTTATCGTGATCTGCATGAACGTCTGTTTTTAAATACTTTACGAAAATGAACCTTCCTAAAATAACTAAAATTAATCCCAATAGTGCTGATAAACCAATTATTGCAGCTTGGATTCAAGGAATATTAGTTTCAGAATAAGCTCTTAATCCATGTAAATAACTATTGGATCAATCAAAACTTTCTTTAAAGAAACCTACAGTGCCAATCATTAGAAACATTCCCGTAGCAAATGCATAAATATAAATATTTTTCTTATCAGTAATTTTTAATTTAAATATAGAAATTACGCCCAAAACTAATGAAGGTACGCCCAATAATATTAAAATATAGATTATTAGATTTACAAATGTTGCTAAAGTTATATTTCCATTAAATGCACTTGTTCCTATAAACATGAAATTCCTTTCTATTTCGTTTATTATACAATAATTTTTAAACAACTATACAATAAAAAATGATAAAATTGTTATATATTATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCTCACTTAGCTTTATGCCTTTAATTATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAGTTTTTTTGCATAGGCTAAGCATAGGTCAGATTGCTGCATTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGCGATATTAATAATTTTTTAAACCAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGATATAGCATATTAGGCGATAAATGAAAAAAAGACTTTAATAGAAATAATGATCCTTATTTTGAAGATATGCAAACTGTTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGATTAAATGATGAGGAACATGAGCAACATCGTCAATGATTCAAAAGATTTTTAGTAATATATTTGAATAAATTTAATGTCAATGTCGGGGATGTAGATATAGATTGGAAAAATGCAAAAATTGTAAGGTCTTTTTCTGGAACCACTGATTATATAAAATTTAAATTCAAAGATATTAAAGATTGAAATGGAAGATCATTATTAAGAAATGATAAAAAAAATATTGAATATTACATAAATGGTTTTAGAACATATGCAACTAATCAAAAATTCGGTGTCGGCAATCAAGGTTTAAAAGAAGAACTACCATTGTTCAACGATTACGTGCCAAATCCACTTCTTGAAATTGATGGCATGAAATTTATGAACATTATTGATAATATTAACTATTTTGTTAAAGGCGCAACTGATATTAATTATTGAAATGCAAAAGGATTAATGTATCTTTTTCAAAGCTTCAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCTGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACTCCATATTTTAAAACAAATCAAATTTTTAAAGCAATAGTAAGAGTATTTAAACTAAATGGTACATATAAAGATTATGTTTTAATATCTTCCAATTTTGATGATCACGGTCACAGATTAAAAGGTTTAATTACTAAAAATGCTTTGCCAAATGAACTTAAAGCTTCTGATATATATTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTAGATGATTTCATTGATAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGAAACAATAATAATAAGCAAAATTATGAAGTTGCTAATTTGTTGCTTGCCAAAGATCCATTCCAATTAAAATTGTTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCACTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTACCAAATCAACCAATTGGAAGATTATATTTAAAGATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGAAATGGATTTAAAGGATATGACCAACAAAAATATGGAAATGAATATTTTACCATTGATAAAATTACAGACGGAGATAATTAAACATGAAAAAATCAATAAAATTGCTATCCTTGCTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCACGATAACGAAGACAAGGAAAATTTAGATTTTTCAAGTGATTTTAGTGGCAAAAAGCAAAAAAATGAATTCAATTTCAAGAATAAGATTTCCCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAACACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAATTGATTAGAAATAAAATCATCAATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCTGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAAACAATTGATGACAGACTTGAAGGTAAAGACATTGATTTAGGTCAAACTCAACATGCATTACAAGCGGTTTGAGATTTTATTAAAGCACATATATTCAATCCAAATAAATTAACCAAGAGAGAAATGCTCGATAAAATTAACATTGAAGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTAGCAAAAAATAAACTTCAAAATTACGAAAAATTTCAAGAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAAATTGAAGTTGGGAAAATTCTTACAACTTATTAAATGATTCATTTTTTACTAGAGCAAAAGAAATTTTAAAAATATTAAAAGAAAATTTGATAAGTATTGCTAAGATTGTGGGTTTAACTGACAAAATCAATTTAGACTAAACCTTTAAATCATAATTCCTTTTTAAACAAAAATAAACGTAACTTGTTATCTAGTTTAATTATTTTTAGTAAAAATAAAAAATATTTCTTAGTCTTATATTGCTAAGAAATATTTTTTTAAATTATTAATCTATTTAATTTTTGCTCTTTTGATCGCTTAATGGATCGAAAGCAACGTAAGGATATTTATCTTTTAATGTAGATCATATGTATGAAGTATCAATGTTTTTGTTAGCTTTTTGAATAGCTTCGCATTGTTGATATGAATTTATTGCATCATATATTGCTTTATATGTTGTGGGGTTGTTTTTTTCTCATGCAAAATATTCTTTGATAACAACTTTATCAATTTTGTTTTCAGTATAATTTGAAAAAAAGCAATGAACTATTTCATGTAACAATGAATAAAATATATATGCTTCAGAATTAAACATATCAGAAAGGAAAATATACCTTTTTGCTCCTTTTTTCAAAGCAATAGCCCTTATCAAACTATTTTCAATAAAAGGCATCGTTACTAGATTAATTCCTTTGCTTAATAAAACTTTTTTAATTGTTTCCATTTTTTCTTCAAATGAACAATGTGTATTAGACATGATCGTTAAAATTTTATGATAAAGTGAATTAAATGAAGATTTTCTAAAAATTCCCACGTTTTCGTCAATATTTTTATCAATTGAAAGCAATTCGCAAAATCTTATTCAAACTTTTGTATTTGGGTTTGAATATGTTCATTCTTCTGCTGAAACATTATCTTTTAAATATTTATCATAGTTAGCCAAGTCAGCAACACCAAAAAATCTTTTTAGCATTATTAATTTAACATGTAGTGGACTATTTTCATGAATGCTAATGTTAGCTGCCAATGCAAGTTCTGGATGATTTATTAAAAAATCAATACCATGTTTCTTTAAATCATCTTCAATATTCTTATTTTTCTTTAGTGTTTTTAAGTTATTATAAATATGATAAACTTCCGTTAATGTGCCAATTTCTAAGTGAAATGCATATTCAAGCGCTTCAATAATAGTTGCACTAACGTTATTAACTTCATTATTCATAATTGAATTGATATGTTTTATTGATAATCCAAGTCTTATAGATAATTCTTTTTGAGTCATTGAAAACATTTTTAAATAGTATTTTATTTCATCTGTAAAATCATTGCCATGAATTTTAACGTGCTTCATATCACAGATGTGCTCTTCAATCATAAACCCGCCTTAACTTAAACTATTTTTTTATTTTTTATAACTATATTATAATTTTTTTTAGTTAAAAAAGTTTATTTTTGATACAAAAAAATATACAAAAAAGTATTTTTATATTGTTTTTTTGTATATTTTTTTAAAATGATAATTATTGTTAATATATTAAATTTAATATTCCTAATCCACTATATCTTTTGGTGCAACTGCAAAAGGTAGAATATCTTTCATTTTATAATGCTTTTGTTGCTTTAAATCAAAACTGTATAAATATACATCGCCATCTGGTTTTAAAAATTGACTAATAACTTGAGCACAAGCACCGCAAGGATATAGCAATTTTGATAAATTGCTAATTATATGTATTTCTTTAAAATTACCAACTCTAGCGCCATATGCTACAGAACCAAATAATGCACTTCTTTCAGCACATAATCCAGATGGAAATGCAGCGTTTTCAGTATTGACTCCCCAATATTCTTTACCATTTTCTTCAATTGCACAACATGCAACTTGAACATGTGAATAAGGACTAAATGAATATTTTAATTTATTTTTTAATTCTTCTATTGTCATACATATAATTTTACAATATATTTGCAATATAAAATTTATATTAAAATAAACATTTTTTAAAAAGTGTTAAGTATTTTTGCTTAACAGTAAAAAATGTGCTAAAATATATACTAACTTTAAAAATAAAATTATTAATTAGAAAGGAAAAACCATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCTGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGGACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAATTTTAATAAATTATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAAATTATTCATTAGATAAACATCATAAAGTTGATGATAGCCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATTGAACCAATAGATTTAGCACTACAACCTTTAAAAAATAGTTACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCAAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTAGATGAAGAAATATCAATTGGAGATTTTATCTTAACAGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATATACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGAAGATTAAAAAAAGCTCGGGAAATAACCAGAAAAAAACGTCCTGAATTATTAAAGAAAGGGAAATAACATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGTACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGGGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGTAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCAAGATTAAAAGAAATTAAAAACTCAAAATAATAAAAAAATAGGGCGTAAGGCCTTATTTTTTTATTATTTAAGGCAATAAATTATATTACTTTAATTAACAAATTATTAACATTTTATTAACAAAAAAATTCAATATAATTATTGGTAAAAAATTTTGCCATTTAATTAAATTAAATACAATTTTTAATATCTTTTTTTTCGACTTAAACTATAATCTTGTTCGAAAGGAAATATTAAAAAATGAAAAAACATACAAAAATTTTATTAGCATTAGGAAGCATTGCAACTTCAGTTGCCGCAATACCATTAGTTGCTGCAGGTTGCGTAAAAACAAAAAACAGAAGAACCTAAAAAACCAGAAGATCCAAAGAAACCAGAAGGTGAAAAGCCAGGAACAACACCAGAAGATCCAAAGAAACCAGAAGGTGAAAAACCGGGAACAACACCTGAAGTTAAACCAGAGGATAAAAGATCAGAAGAACAAAAAGAAATAGATAATTGAATAGAAACTATTAAAGACCAATTTGAATTTTCTCCTTACAACAAAGAAGAAGTATTAAAATCATTAAAAGACAAAACAGGAATATTAGTATATAGTTATATATCTCATAATATAGTTTTAAAAGATAATAATGATAGTAAAAATTGGTCTAAAATTGTCTTTTCTCCTAAGGATAAATCTTTATTCTCACAATATCAATTAGCCAATCCAACAAATCCTACTTATTATAATAAAAAACGCAATAATAATTCAATAAGTGGTTACATTGACTATGAATTAAAAGACAATAAGATAACATTAAAATTTAAAGGGGCTAAATTTGTTGTAAAAAAAGACCCTATTATTGGAACTAAAATTGTAAGCAATGAAATAGATCTATCAGAAACACAATTAGATAAAATTATAAAAGATCTTAAATTTGATATCAAAGACAAAAACAAAGAAATTAGCAAATTAACTGCCGATGATGTTAAACTAGAAGGCAATCTAAATGGCGCAAATATTACTTATAATTTTGCAGCAAAAAAAGGCGATGAAACAACACTTGTTTTAACTGCTAAAATTTCAAAGAATTCCGTAACTCAAACTTTTGAAAGAGAAATTAAAGGATTTAAAAAACAAGAAGCAAAACCTGGAACTAAAAAAGAAGACGAAGAATTAGATCCAAAAGTAAAATTGATCAACAAAAAAATTGAACAAAACAAAGACTTATTTACATTCAAGGTTTGAGATCAATCCAAAAAACAAGATGTAATTGAAAAGCTAACAAAAAAAGAAGCTAAATTATTCTATCTAAAAGGAAATCATACTATTATTATTGAATTTAATGGTCAAAAACCAGATGAAACCTTAAGCCAAGTTGTATTTAAGGGAGAATCTGGAGCATTTTATTCATTTGGTCAAGTTCAATTAGTAAATCACCTTCACCACTACAAAGATGAAAAAGTTGCAAAGAATAAATTAGAATTATCTTACTACTTGGAATATGAAGTAAAAGATAATAAAATCACCATAACTTGAGCTGGTGGACAAAAAGATTATGGAGTAGAACCTTTTATCTTTGGAACTAAAACTGCTTCACAAACAATTGAATTAAAATAATAAAACAAAAAAGCGGGCAACATTGCCTACTTTTTTATATGTTTTTTTAATAGGAATTTATTAAATTCTTTTAAGTATGTAAAAGTCTTTTTTACCTTTTTTTAATAGCGCATATTGACCATTAAATTTATTAAATGTAATTGAATAATTTTCATCGTTTATTATATTGTTATCAATTAAAAAAGTATTTTTTGAAATAAATTCTCTTGCTTCTCTATTCGAAGAAACAATTTGATTATTTTTTAAAATTTCAATAAATTTTTCGCCTTCTTTAACTCCAATACATGGTAAATAGCCTAAAAGCATCGAAATATCTTCTATGTTTAATTCTTCGTTACTTTTGTTGAATAAAATCTCACTTATTCTAATACATTTGTTTGCTATATCAACTCCATGAATGTCTTTAACCAATTCAAAAGCTAATTTTTTTTGGGCAATTCTTTTGGTTTTATTTTCATTGTGTTGAGTCATAATTTCATTTATTTCTTCAACTTCTAAAAATGTTAATCATTTTAAATATTCTTCGATTTTTACATCACTTTGATTCAACAAGAATTGATATATAGAATATGGACTAGTCATATTTGGATCAAGCCATAACGAACCTCCGCCAGTGCTTTTACCAAATTTTTTACCATTTTCATCAACTAATAAATTGAATGTCATGGCAACAGCATCAGCAGATTCTCCATGTAATTTTCTAATAATTTCTAGTCCAGTTGTAATATTTCCTCATTGGTCTGAACCACCCATTTGAATTTTTACATTGTGATTTTCATATAGACATTTAAAATCTCAACCTTGAAGCAAGGTATATGAAAATTCTGTAAATGTTAAACCATTTGCAAGTCTAGTAGTAATAGAGTCTTTTTCAAGCAAATAATTTACATTAATTAATTTACCAATATTTCTTAAAAAATCTACTACTGTTCAATGCTTATAAAAATCTAAATTATCAATTACTTCTAAACCAAATTTTTGTAATTGATTTTTAATTGCATTTTTGTTTTGTAGTAATGTTTCTTGATCTAGAAATTTTCTTTCAGAATTTCTAAAACTAGGATCACCAATCATTCCGGTTATTCCACCCAATATTGCAATTGGTTTATATCCTGCTTTTTTAAAACGTAACAAACTTAATATTTGTAGATAATTTCCAAGGTGTAAACTTTTAGCAGTTGGATCAAAACCTGTATAAACACCAGTTCCTAGTGATATATTATTAAATTTTTCTTCACTAGATATATTATTTAAAACACCCCTTGATTTCAAATCATCTAATATATTTTTTTGCATATCTGTCCTTTTTGTTATAATGTATTAAATTATTTATTAAAAATATAAGTAATATAGTAATTATATATTAATTTTGTTTATTTTATGGCTATGAATCTTAAAATAAACAAATAGAAGGACATTATGAAAATTAAGATTATTATAGACTCATCAGCAGGGATGTCGCAAGAACAAGCAAACAGCTTTGGATGAGATTTAATTCCAATTCAAATTGAAGTAGGAAATGATATATATAAAAGTGGTATAGATTTTGATACAGACAAATTTGAAGAACTTTGAAGAAATGACAAAAAAATAGATGCCAAAACTTTTGCAACTCCTCCAGGAATAGCCATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAATCTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTCGATGAGGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCAGAGTTTAACGATGCCTTACTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAATGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAAACAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCGTTTAAACAAATAACCAATAATTTTCCAAGGATTTATACAATTCAATTGAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCTGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATACTTAAAAAAACAATAAATTAAAAATACCAGGTTTTTACTTGGTATTTTTAATTAGTTTATTTCTTGCTTTCAATTGGGTAAACTGATGCAAATTTTCTGTTAATTCTGCTTTCGAATTTTACAATACCATCGATTAAAGCAAATAAGGTGTCATCTTTTCCTCTACCAACATTTTGGCCTGGGAAAATTTTTGTTCCTCTTTGTCTATAAATAATGCTTCCTGCTAAAATAAATTGGCCGTCGGTTGCTTTTACGCCTAATCTTCTTCCCGCTGAATCACGGCTATTGGCGGTTGTTCCACCTGATTTCGTATGTGCCATAACTATTTTTCCTTAATTTCTGTAATTTTAACTCTGGTAAATGGTTGACGATGTCCTAATTTACGTTTATGAGTAGATTTAGCATTATGTCTATAAACAACTATTTTTTTTGCTTTTCCTTGTTTTTCAATGGTTCCTAAAACAGAAGCTTTTGGTAGATAAGGTGAGCCAACTTGTTCATCAATAACTAAAACTTTATCAAATGAAACTTTGTCACCTTCATTGCCATCAATTTTTTCGATGTAAATTACATCACCGCTTTTAACAAGTAATTGTTTTCCGCCGGTTTCAATAATAGCAAACATTATGAAGCCTCCTTATATTTTGTCACATCCTTAAGGTACTATTGATATAGTTTTAAAACCTTTTTAGTATGGTTACTCTATAAGTAACAAATACATTATAGCAAAAAAATATTTTTTTAATCAGTAAAATTTTTTTCAAAAAATTTCTTTTATTTTTCAAGCAATTTACTTACTTTTTTAATTTCAATATCTAGTTTTTCTATTGAAGCAAATATTTCATAAATTGTTGAACTTTTATCCTCTTCGGCGTTTTCTAGTAAGAATATTGTAAATGCATCGACTATAATATTTTTCAAACTTAATAAATTAATTTATTAAAAAAACTTCCTATGTTAATTAATAGTAGTATTGTTGAATTAAAAAACAATATATTTTGGCAAATAAAAATATTATTTCAAACCTTTTAGTTGATTTTAATTGCTTTTTGAAAGCATAGAAAATGTGGGATTAATCAAAATAAAGAAAAAAAACAAGATCATAACTGTCTTGCTTTTTTCTATAATTTATAAAAAGAAAGACTTTACATATTTTTTTATAGAGTATGAAAATCTATTTTATAGATCTTTCTTTTTTATTTTTTAATTTGCTCACTGCCTATTTTAAGACCTTCTTTTATTCCGTTTAATTTCTCTTTAATTTCCATATTTCAAATTTCTTTGTCTTCATTTTACAATTTTTCATATTCTTCAATAATATTTCTTGCTTCATTAACATAATTTTCTCTTAAATACGTTATTAAATCGTGAAATCTTCGATCTTTTGTATTTTTATAATTTTCTTGTAAAATTAGTCTTAATTTTCTATTGCATCTATATATGCTTGGATTAAAGATTTCACAATTGCTTTTATTTGCATTTTAAATTCTTTAACTTTGCTTCTTAATTTCCCAAAAACAGATTTATTTATTTTTTTGTATAATATAAGTGTAAATAATGAAATATAAAAGATAAGGAGATAATGTAATAGAAATAGTTGAATATTTACAAAAAAGACAATTTAGATAAATTTTTAAAAAGAGAAGATTTGCCAAACTTTTGACCTATTTATGTTTATTGTGGGTCTGCCGGTTGAATTAAACCTTGAAAGTGAATTAGGGATGGATATCCTTGCTTAATATTTGGACTTACATACCCGATACTTGGGATGACCAAAAAATAGGACATGCCGTTGTTGCATATGGTATGTATGATAATGGCAATAAACTTCTTTGCCATTATGGGTGAGCAGGCAGTTCTCAAGTTATTGTTTCTTCGAGTCTATCTGGCCAATTATTCTTATTAGGTATGAAGCCAAATGGCGAAATTAAAAAAACAAGACAACATTTTACTAAAAATGGAATTAAATATTCGGGAATGGATTTTAAATAATGAAGAAGATTACATTAAAATGGTTAACAATTTTAGGGGCAACAACGCTTACTGCTTTGCCTTTTTGTGCAATTTCTTGTAGTAATGAGAAAAAAGAAATCGAAAAAGCAGAGAAAATTATTGGCGATAAAGAACTAACATTTAAAATAGATGATTTAAAATATGAAAATTCTGATAATTCTATATTGCAAAGTGCTAGAAGTATTGCAGGACCATGGCGCAAGGACTTTCTTAAGGTTGTTATAACGTCAAAATCAAATATTGAAGAATATTGAAAAGATAATTGAATTGTAGGAAAAATAGATTTAAAAAATAAGGCTATATTATTTTTAATACCTAGAGAAGTATTATTAGATGCTGATTTTATTAAAGAAAAAAATATGGATCTAAAATCTTCTTACCAAAAAGCAAACAAAGCATATTTAATAGAAAAACCAAAAAAAGTATATTCAAATCTAAAAAATAAACAAATATTAATAAATAACTACACTATATTTTTTGATGATATAAATAATGATAAATCTCCAAGAGTTTCAATGGCGATAAAAGAATTAAAATTTAATTTTGATCAAGACCGAAAAACCATAGAAATTAAACTTGGTTTAAAATATGAATTATCACAAAAACATAATAGGAAGTCTAATATATTTTTTCTTTCTCCTAAAGCAACCTTTAAATACATTTTAAGCGAAGAAATCAAATAAATTTATTTAATCAGCAAATATTGATTTAAAGCATTAAAAAAGGGAATCATAGAAAAATCTGTGGTTAATTCAAATAAGAAAAAAAGCAAGATCATAACTGTCTTGCTTTTTTCTATTATTCATAAAAAATAAGTTTAAAAAAATTTAAAAATCTTTAAAAGGAATTTATGGGTGCTAATAAAGAATTTATTTAATGCAGCAAGAGGCTAAATTAAACAACGATTATTCAAAGCTCTTTAATTCTTGTCAAAACCAAATTAAGAATTTAAATAATTTAAACATCGAAAATAAATTTCTAAAACAAAGAATTTTAAAAATTATAAATAATGAAATAAATGATTTAAAAAACAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGTAAAATAATAATGATTTACTAGCCAAAAGAATTTCGCTTCATTTTTTAATGAGTTTTTAAAAACAAAGTTAACTATTTTATTAAAAACAATAACAAATAAAAAACACCCTTATAATAGGGTGTTTGTGTCAATAAAATTAAACTCTTATTTAAATTTTAATTATTCAAAATCGTTTTGATTTTCTTCATCATCAAATGTTTCTTCTTCAGCAACAAGTTCTTCGTCGTTTGGAGAATAATCTTCTTCAACTGATTGTTTATCATTGTTATATTCTTGCGCTGTATTAACAACTGAATCAATATCAATAGGTTCAGAAATTTCTGTGCCGTCAATAGGAGTTATTGTAAAGTAATTAATTGATGGTTTTATATCATATTTTGAATGTTCTTCAAATGGGAATAATGCTGTTCCTGATGGAATCTTTTTACCAAGAATAATATTTTCTTTTAAGCCAGATAAATTGTCAATTCTTGATGAAATAGCAGCGTGAACTAATATCTTGCTTGTTTCTTGATAAGAAGCAGCGGCAAGGAATGAATCAGATAACAATGGAACTTGTTTTGCACCTTTAATAACAACTTTACCATATGCTGGCTTCAAACCTTTTGAAAGTAGAATAGCATTTTCTTCTTGATAGTCAAAAATATCTACAATAGCACCAGCGAAGAATTTAGAATCTCCGGGTTCAGAAATTACTAATTTAGACATCATTTGACGAATTATAATTTCAATATACTTATCGCTAATTGCAATACCTTGAATACGATAAATTCTTTGAATTTCTTTCAATAAATAATTTTGAACAGTAATTACGTCTGTTAAAGCTAATAATTCTTTTAAGATAATTGGTCCTTCAGAAATCTTTTGACCAATTCTTAATTCATCACCTTCTTGAACACGTAATTTTTTGGTTGTATTAACAAAAATTTTGTGAGAAACTGTTGTGTTTGAAGATCCTTTGCTTTCAATTGTTACTAAATAGTTTGTATTGTCTTTTTCATCAACTTCAATCTTTGTAACAATACCTTTATATGGTGAAATAACAGCAGGACGTCCTCATGGTTGTTCGTGGGCATCAATTAATTCGATCAATCTAGTGAAACCACCAGTAATATCTTCAACCCCGGCAACACCACCAGTGTGGAATGTACGCATAGTTAATTGAGTTCCTGGTTCCCCAATAGATTGAGCTGCAATAATACCTACTGCTTCACCGATATTAACAATTCTATTTGTTGCAAGGTCTTTACCAAAACACATCTTACATACACCGTTACGTGTATTGCAGCCTAAGATTGATCTAATTTCTACTTCTTCAATACCACTTGCTATTATTCGTTGAGCAATTTTTTCAGTAATTAATTCATTTCTAGAACAAATTTCTATACCTGCGCTTGTAACAACAGGTTTGTTTGCAAATCTTCCAACAATTCTTTCTTTCAAAGGAACAATAATTTGTTTAGTTTTTGTATCAACTATGTTTCTTGCTTTATAACCATAGTCAGTGCCACAGTTTTCTTCTCTAACAACAATATTTTGAGCAACATCAACTAAACGTCTTGTTAAATAACCAGATTTTGCAGTGTTCAAGGCAGTATCGGTTAAACCTTTTCTAGCACCGTGAGTTGATGAATAGAATTCAAATGCAGTAAGTCCTTCAATAAATGAAGACTTAACAGGAACTTCAACTGTTGATCTAACAACACGGTCGTTTTTAGCATCGGCCTTAGTAGTTTTAGTATTGTTAGCCATCAATCCACGCATACCTGCTAATTGAACATAGTTAGAAATGTTACCACGAGCACCTGAGTTAATCATGGTAATAACTGGGTTTTGAGGATTTTCTTTAATTATTTTTTGTAGGTCTTCTTGAATTAAATCCTTAACTTCTGCTCATTTTTTAATAGTTAAAGAGTAACGATCATCATCAGTTATTCAACCTTGTTCATAGTAGTGTTTTAATTTAGCAATATATTCATCACCTTGAGCAATATATTCTTGCTTTTTATTAGTTTCTAGAATATCACTAAACGAAATAGAAATTCCAGAATATGTTGAATAGTCAAAACCTAATTGTTTAATACTATCCAATATTGAAGCAACAATGTTTGTATATCTAAATCATACAGTGTCTAGAATTTCTGCTTTTTCAGCTGGACTAAGTGGAACTTCTTGTCCATTTGCCCTAGCAGGATTTAACATTTCGATCTTTTGCTTTTCTTGACTTGTAAATTGAGCTAGCAACATTGCATGTTCATAATCAATTTTTCTATTTTCATAATTTTTAACATGCAAGAATTGAATTATTGAATCATTAGCATCACTTGATTCAGTTAAATTATTAATAACTTCTGCAATATCAGACATTGAAATTTGAGCAACATATTTATCAAAAACATAACGTATTATTTTAGAAATATTCTTTTTGTTAAATGCTTCTTCAATAGGCATATTTTTGATATATTCAGGAATATTAGTACCTGCTTGAACGACATATTTTGCATTGAATGTTTGATTATAATCTTCCAATGAATTTGCCTTAGCAACTTTATTGTCGTAAATAAATGGAAATGTTTTAGGAAATATATTATTAAAAATAAATTTACCTACTGTTGAAATTATATATTGAGGACCATCAAATGGGAAAATAAGTTTTGGAGTTTTAACTTCTGAAGCAGGTAATGCTATTCTAGTGTGCAATGTAACTACTTTATTTTCTAATGCTCTAAGCATGTGTTCATAGTTATCAAAAACACGTCCTTCACCTTTAGCCCCTGGTTCTTCAATAGTTAGATAATAAATACCTAAAACCATATCTTGAGAAGGGTTAATAATAGGTTCACCATCTTTAGGTCCTAGAATGTTTTTATTAGCTAACATTAATTCACGACTTTCAAGCAATGCTTGTTCAGAAACAGGAACGTGAACAGCCATTTGGTCACCATCGAAGTCAGCATTAAATGGAGTACATACTAATGGGTGTAATCTAATAGCCTTACCTCTTACTAAAACAGGTTCAAAGGCTTGAATAGATAAACGGTGTAATGTAGGTGCACGGTTTAACAATACTAGTCTGCCCTTAATTGCTTCAGCAACATGAGGTCAAATTACTGGGTTTTGGTCTTCAATAATTTTTTTAGCACTTTTAATTGTTGTAGCTACTTCTTTTTCAATTAGCCTTTCAATTATTCATGGTTCAAATAATTTAGCAGCCATTTCTCTTGGTATACCACATTGGTGCATTTTTAATGAAGGTCCTACGGCAATAACTGAACGTCCAGAATAGTCAACACGTTTTCCTAGTAAATTTTGTCTAAAACGACCTTTTTTACCAGTTAATGCATCTGAAATTGATTTAAATGGACGATTGTCTTTTGATAAAACAGGGTTAGGTGTTCTTCTTTGGTTATCAATTAAAGCATCTACTGCTTCTTGAATCATTCTTAGTTCGTTTTGAATAACCAATGTAGGGGCATCTTTTTCTTGTCATTGTTGCAATCTATTGTTTCTAATAATTACTCTACGATACAATTCATTAATATCACTAGTTGAGTGTCTACCACCATCTAATTGAATTAAAGGTCTTAAATCTGCTGGAATAACAGGCAAATTATAAATTAACATGCTAGTTGGATCTTGCTTAGATTCAATAAATGCATTAATTACTTGTAAACGTTTATAAAGTTTTTCTCTTGTTTGTTTTGCAGTTGTGGCAAAACGTTCAGGATTATTAATTTCTTCTGCATTTAAACGATTAATTTCATCAGTTACTTTTTGCTTTTCTTCAGCTAAATTTATGTTTTTTAATAAATATTCAATAGCTAAAGCACCAGTACCTATTTTAGCGTCTGAATAATGTTCAATTACTTCGTTTAATTCATAAAAATCAATTCCATATTCTTGACCGATTTTTGATGTTGCTTTTTCAATTAAAGAATCTAGGGTTTCTTTAATATCATCATATTCTTCTGTACCTTCTTGAAATCTTTCAAGCAATTCTTCTAGAGCATTGCGGTAAATGATTGCCGCTTCATTGATTTCAATAATTTGATTTTTAGGTAATGATTTTAAGCCACCATCTTCTAGAACAATATGATTTTTGTAATAAATTAAATTTTCTAGTTCTTCACGTTTAACAACGTCAGAACCATTTGAAGATTTTAAACCAAGCAATTTATAAATAATTGAGTGATCAACTTTGAAATATCAAAAGTGAACAACTGGACTATTTAGTTTTATGTGTCCCATATGGGTTCTACGTGCTATTTTAGGCAATATTTGAACATGTTCTTGTTTACATAATTCAGTTCTTGTACAATAGCTTCCTTCATTAACTTTTTTGTATTTGAAACCACATACAGGGCAACGATAATCAATCATTGGTCCAAAAATTATTTCATCAAATAATCCACCACGTTCTGGCTTATATGATTTATAGTTAATAGTTTCTGGCTTAGTAACTTCACCATGAGACCAAGTTTCAACATCTTGAGGAGTTGCTAATGCTAATGAAATTTTTGAAATTTTTTCTTCATCTAATGATTCGTATTTTGAAATTTTTTTCATATTATTCTAAACTCCCTTCAAATTCTTGGAAATATTTTGTGTCTACTCTTTGATCTTCTTCTGAATCATCTACAACATTATCAGATTTTTGATGTACTTCTAATTTAATTCCTAAACCACGTAATTCATATGCTAAAACATTAAACGATTCAGGTGTTCCTGGTTTAGGCAATCTTGCATCTCTAGCAAGAGAGTTATATAGTTTATTACGTCCAACAATATCGTCAGATTTATATGTTAATAATTCTTGTAGAACATTTGAAGCTCCATATGATTCAATAGCTCATGTTTCCATTTCACCAAATCTTTGACCACCATTTTGACTCTTTCCACCAAGTGGTTGTTGGGTAATCAATGAATAAGGTCCTACTGAACGTGCGTGCATTTTGTCATCAACCATGTGATACAACTTCAAATAGTACATAATCCCTACAGAAATTGGATGATCAAATGGTTCTCCGGTAGCCCCATCGTATACTATTGATTTTCCTGTTACATCAATATTTGCTTCTTCAAGAATATCACGAATTTGGTTGTGTGAAATTCCATCAAATATTGGTGTAACAAATTTGGTATTTAATTTTTTAGCAGCAAGTCCTAAGTGCAATTCTAGAACTTGACCAATGTTCATACGTGAAGGGACACCTTGAGGGTTTAAAACAATATCTAATGGTGTTCCATCTGCTAAATAAGGCATTTCTTCAACAGGCAATACTATTGAAACAACACCTTTATTTCCGTGACGACCAGCCATCTTGTCGCCGACTTGAATTTTACGTTTTTGAGCAATTGATACCTTAACTATTTTTTCAATGCCATCTTCTAATTGGTCGCCAAATTCTCTACTTAGAATATCAACAGCAACAACGGTTCCATTGTGGCCATGTTTTACTTTTAGAGAAGTATCTTTTCTTGAGGCAGTTTTTTGACCAAAAATTGCAGCCATTAATTTTTCTTCTGGAGTTGGGTTTTCTTCACCTTTTGGAGAAGTTCTTCCGACTAAAATATCACCTGCTGTTACTTCTGAACCAACTCTAACAATTCCATTTTCACCTAAGAAACGTTTAGAATAGTTTGAAACGTTAGGAATATCTGCAGTTAAAATATCTTCGCCAGCCTTTGAATGTCTAAATTGAATTGATTGTTCTTCAATGTGAATTGATGTATAAACATCATCTTTAACCAATCTTTCAGATAAAACAACGGCATCTTCATAGTTATAACCATGTCAAGTAGTAAAACCTACTAGAACATTTTTTCCTAATGCTAATTCTCCATCTTTCATCGAAGGACCATCAACTAATAAATCTCCCGCATTAATTTGTTGGCCAACTTTAACCAAAGGTACTTGGTGAATTAATGTACCTTGGTTACTTCTTTCAAATGTTCTTAAATGATAAACATCACTTTTTGAACTATTTTCAGGAGTTACTTTAATAACGTTAGCATCTACATAGTTAACAACACCTGATCTGGTTGCTCTAATATTGGTTGATGAATATTTAGCAATATCAGCTTCAACTCCTGTAGCAACTAATGGTGCTTCTGGGAATAACACTGGAACAGCTTGACGTTGCATGTTTGAACCCATCAATGCACGGTTAGCATCATCGTTTTCCAAAAATGGTATAGCCGAAGCAGCTATTGAGGTCATTTGACGGTTTGAGACACCAATAAAATCTACATCTTCAGCATTTACTTCTAAATATTCATTGTCACGTCTTACCATTACTTTTTCATCAACAATTACATTACCTTCAATGTGAACAGTTGATTGTGCAAATGTATATCCTGTTTCTTCAATTGCAGTAATGTAAACTGGTTCTGAAAAATCTACAACTTTTTTATTTACTCTGAAATATGGTGATTGAATAAAACCATATTTATCAATTTTAGCAAATGATGCTAAATTTAAAATTAAACCAATGTTTGGTCCTTCGGGTGTTTCAATAGGACAAATTCTTCCATAATGGGTAGGGTGAACGTCACGAACTTCAAATTGTGCAGTATCACGGTTCAACCCTCTTGGTCCTAGGGAGGTTATTCTTCTCTTGTTTGATATTTCAGCAAGAGGATTACATTGATCCATAAATTGAGAAAGTTTTGATGTGTTAAAGAACGATTTAAATTGATTAAATATTGGTTTATTATTTGTAACGTTCTTAGCAGTAATCTTATCAATATCTTTTGCTGAGATTCTTTCTCTTGTATTTTTTTCTAATTTTAATAGCCCTATTCTAAATTGATTTTGTAATAATTCGCCTATTGTTACAATTCTTTTATTAACTAATGAGTCAGGATCATCATCAACCCCAACCTTTGAAAGTAAATTAAAATAATACGAAATCGTTGCAATTAAATCAGGAATCAACAAGAAATTTTCTGTTGATGTAGGATCATTACCTAAAACCAAAACTTTTTGGTCATGCAACATATCATTTTCAGTTGGATATATTCAAATGCTTGTTACAAAGATTCTACTTGCAAGTTGTTCATTTCCTTCAACTTCAAGTTGAGATCCATATGCATTTTCATCAATATCTGGCAATTTTCACATAGGAATAATTCCTTGTTTAAATTCATCATGAATCTTTCTTGCGATATTTCAAGTAATGAACATTCCTTTTTCATAAAGAAGTTCACCTTCACTAGAAAAAATATCTTCTGCTAAATAAGAATTAGCAATTCTTTCAATAATGTTTAATTTTCTATTTAAAGTGAAACGTCCTGTTTCAGTTAAACTATATCTTTTTTCATTAAACAAAGTTGTAGGAATTAAATTTCTAGCAGAATCAGCTGTCATACGATCGCCTTTTCTGATGATTCTATAAATTGCTTCTTGTGCTTCTCTTGTAACTTCTTCTGCATTAATTCCAGTGATTTTATCACGTTTAAATGTTTCTTCCAATTCTGGAATATTTTCGCCAAACATTCTTCTAATACCTGATTCACTAAATCCTAATGCTTTTAAGAAAGTTACAACTAAAAATGATTTGTTTTTATCGATATGTATTTTTACAGTATCTGGATTTTGAGAAGTTACTTTATGATAAATTTCTACTCATGAACCAATTTGAGGAATAATTTCTACTTTATTAAACAAGTCGTTGGCTTGTTTATTTCTAACATTAATTCCAAAATAAGCCCCGGTAGATCTAATTAATTGCGAAACAATAACTTTTTCTGAACCATTGATAATAAATGATCCACCATAAGTCATTATAGGAATTTCAGCAAATACTACTTCTGAATCTTCAACTTCACCTGTTTCGATATTGATTTGTCTTAGGTGTGCTTTTAACTTTCCTTGATATGATGAACCTTTTTGTTTAGCTTCTTTAATGCTTTCATATTCAGTTTTTTTAGGATATTCAAAATGTGCTGAATTATGAATGTATTCAATTCTAACTTTTCCATGTGCTTCAATTGGATAGATATTTCTTAATTCTTCTTCAATTCCAACTGTTAGAAATTTTTCAACTGATTCACGTTGCATTTCAAGAAAGTCAGGAGTTTCGAATTTCTTTTGACTAATTGAATAGTCACGTCTTAGGGTAATAGGTCCAAATTTTCTAATTTCGTATTTATCCTTATTCACCATGCTTTACCTCAATTTTTATTAAATTTTGTTTATATTTTTATTTTTTGTTGTATATACGCACACGTGCATAATATACGACAAAAAACACAAAAAAGGCTTTGTGTATTAAAAGCCTCACTTGTGTTTTTTGTAATTAGTCTAAAGTAACTTCGGCTTTAGCTTCTGTTAATTTAGCTTTTAGTTCTTCAGCTTCTTCTTTTTTAGCATTTTCTTTAATTACAACAGGTGCTGATTCAACAAGTTTTTTAGCATCCATTAATCCTAGACCTAATAAGTCTTTAACAACTTTAATAACTGCTAGCTTGTTTCCACCATCTGATTTTAAAGTAACATTAAATACAGTCTTTTCTTCTGCTGCGTCAGCTGCTGCTGCTGGAGCTGCTGCTGCTACAACTGCTGTTGGATCAATTCCAAATTCTTCTTTTAATCCTTGAATTAATTCCATAACTTCTTTAATGTTCATTTCTTTTAATGCTGAAACAAATTCTTCTTTTGTTAATTTTGCCATGATAAATCTCCTTTAAAAATAATTATTCTGAAATTTTTCCTTGTTTTATTAATTCATTTAAACCAATTGATAATTGTTTCAATGGACTCAATAATGAATTTCCAAGAATCATAAGTGATTCTGTAAATGAAGGAAGTTTTGATATTTCGTCTAATGTTTTTTCATCAACTACTTTATTTTCATAAATTCCACTTACAATTCTTAATGCAGGGAATTTCTTCTTTAAGCCTACTAAGGTCTTTAATGCTGCTAACGAATCGCCTTTAACAAAGGCATAAGCATTTTGTAAAACTAAACTATCATTAATATTTGCATATTCTGTATTTTTTAATGCTTGTTTAACTAGTCTATTTTTGTAAACTTTTAGCAATGCATTATTTGGAGCTAATTCTTTTCTAATTTCTTGAAATGAAACAACATCCAAAGTAGCATAATTAATTACATATAATGCTTGTGATTCTTTTAAATTGTTTGAAATTTCTTCAACAACTAAAACCTTGGCATCTTTTAATGCGCTCATTATTATTCCTCCTATAAAGATTATGCAATGTACAAACAAGTATAAACATACTAGGTAACATTATTAAGCACTACGCCGTTACTGTCTTCATATATTGCGTTGAAATTATAACAAATTTTTAAAATTTTTATAATTTTTAAATAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATAACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCGTCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGGTCCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGCCATTTTCTAATTGGCACGTTTATAATTATACATATTTTTTTGTTTTTTTATAAAAAAATAAATTTATTTTATTGTTTGTTTTTTTATAAAGTTTTTAGCAATCAAAATTGCTTGATTTATTATATAACTTTAATTAATTTTTTTTGTAAAAAATAGATATTTTTTTATTTGCTTATATATTTTTTAAGAAATGCTTCAATAATTACTTATTAGTAATTTTTAGTAATTTAATTATTTATTTTTTTAAATTGAAGAGTTTGATAAATAAAAATTCTCTCGAATAAATCAAGAGAATTTTTTATTATTTAATATTTATTTAATTTTGTCGTTAATTGCTTCTTTAATAACTTTTCTTCCTCTATAAAAACCGCAGTTTTCACATGCTTGGTGAGGAGTAATTAAATTCTTACATTGTGAACATTCAACTAATGTTGGAGCCACTAGAGCGTGATGTGATCTTCTTAAATGTTTTCTTTGTTTTGAAGTTTTTCTTTTTGGTACTACTGCCATAATTTTCTCCTTTAAATAATCAAATTATACCAATTCGATTATTGCCATTTTAGCATTATCACCAGCACGATTGGCTAATTTTGTAATTCTTGTATATCCACCATTACGTTCAACGTATTTTGGTGCCACAACATCAAATAAATATTGTAATGAATTTTTATTTTTGTTTTCTGTTGCAATTAATCTTAAATATTTGGCTGCTTGTCTACGCGAAGCTAGGGTATTTTTCTTACCTAGAGTAATCATTTTTTCTGCATTTGAACGAATTCTTTTAGCACGTTCTAATGTGGTTGTTACTTTGCCATTAATCAATAAATCTGTTACAAGTGATCTTTCAACGTGGTCTCATCACTCAGTATTTCTACGAAATAGTTGTTTTGGATTTGCCATTGTTATTCTCCTTCTTCGTTAGCAGCATATTCTTTGTTAGAATCTTCATTATTTAATTGACTATCAAGTCATTCTCTACGACGGTCAATAATATCTTGTACTGATTTTTTACCCAAATTTTTAATATTTGAAAGTTCTTCATCGCTTAATTTCATTATTTCATCAACATTATTAAATCCAGCACGTCTTAAAGCATTCAATGAACGAATTGTCAAATTCAATTTATCAATAGAAACTGAAACTTTTGGAGTTTTTTCATGTTTTTCTTTTTGATCATCGAATAAATCAATTGTTCCTAATGCGTCAATGTTTCCAATGATTTGGAAATGAGCAACTATAATCTTTGCTGCTTCTTCCATAACGTTTTTAGCTTCAACAGTTCCATCGGTTTCAACTTTAATTTTTAATCTTTCTTCAATTATGTTAGAAGTAGAATTTAATTCTTCAAATAAAATCGCAACTTTTTTAATAGGACTGAAGTCACTATCCATTGCTAAAAATTGACCATTTTTGATTTTTGATTCTAATTTTGGACCATATTCTAGAATGGTCTTTTTATTGTCTTCAAATGAAACATAGCCTCTACCGGTTCTTAAATATAATTCAAATTCCAATGAACCATTTTTTGATATTGTTGCTATGTATTGATCTTTATTAACAATGTCAAGTCCAGGGATAGTTTCAATATCCGAAGCATGAACTTCCCCTTCTTTTGTTGATTTAAATGAAATTTTAGCTAAATTATCTTTTTCAAATAATTCTGGGTCATATGCAAAGCGAATTTTTCTTATGTTTGCAACTAATGTAATAGCATCTTCTCTTAATCCTTCAATTGCAGTAAATTCATGTTCAACATGACTAATTTTTATTGCAAATGGAGCAACAGAAGTAATCGAAGAAAGTAATGTTCTTCTTATTACAGTTCCTATTGTGTTACCATAACCACGCATTAGTGGTTCAATAACGAATGTTGTATTAAAATCTGATACTTTTTCTGCTACTAATTCTTTATATGTAATTTTTTGTATTTTCTTCATATTTATTCCTTAAAAAACAAAATAACAAATTATCCACGTTTAAGAATCTTTTTAGGAGGACGAGTTCCATTGTGAGGAGTAGGTGTTACATCCTTCACTTCTTTAACACTTAAACCACTTGTTTCTATTGATTTTCTAGCAGTTGATTTTCCAGGACCTACACCTTTAACTAAAATTGTAACTTCCTTTAAACCAAATTCTTTTGCTTTTTCAACTGCAGCAGCAGCAGCAAGTCCTGCTGCATATGGGGTTTTCTTCTTTGTGCCTTTGTATCCTATTGCACCAGAAGAACTTCAAGCAAAAACATTTCCTTTTAGATCTGAGAAAGAAACAATTGTGTTTTGGTAAGTTGAGTGAATATGTGCAATACCTTGAGTAATAACTTTTTTATTTTTCTTTGCCATTATTATTTACCTTTCTTTCCTGCTATTGTTTTTCTTGGACCTTTTCTTGTTCTTGCATTTTTCTTTGTAGATTGACCACGTACAGGTAATCCCTTACGGTGTCTAATACCACGATATGATTTAATTTCCATTAATCTTTTAATGTTTAGATTTATTTCTCTTCTTAAATCACCTTCAGTGGTGTATTTTTTAGCTTCATCTCTGATTCTAGTTAATTCTTCTTCGCTAAGATCTTTAACTCTTTTTTCGCCGTCAACATTAGCATCTTTTAAAATTTCTTTTGCTAGTGAAACGCCAATACCAAAGATGTAAGTAAGTGAAATACGAACTTTTTTATTATTAGGAATTTCAATGTTTAAAACTCTGGCCATGTTTTATCCTTGTCTTTGTTTATGTTTTGGGTTAATGCAAATTACGCGATTAACACCATGTCTTTTAATTATCTTGCAATTATTGCAAATACGCTTAATAGAAGCTCTGACTTTCATATTTCTCCTTATTATTTGTGTCTAAAAACAATTCTACCTCTGCTCATATCATATGGACTAAGTTCAACATCAACTAAATCCCCAGGGATCATTTTGATATTGTGAAGTGACATTTTACCTGAAATAGAAGCTTTAATAGTAAGTCCATTTTCTAATAAAACATCATAGTCTCTTGATGTGTGCATTTTAGTAATTTTGCCTGACATTTTGATTGCGTCTTTTGCCATTAAATATTGTCTCCTGAGAGTATATATGCTTGGTCATTGTCAATTAAAACCGTTTGTTCATAATGAGCAGTGTTATAACCGAGAGGATCATAAACTGTTCATCCATTTCCCTTGACCAATACATTTTTAGATTTTTGTAAAATCATTGGTTCAATGCAAATTACCATACCATTTCTAATTAGCTGTCCAGTTCCTGGATAGCCATCGTTATAAACATATGGATCTTCATGCAACTTTCTGCCTATTCCGTGACCTGTATATTCATCTGGAGTAAAATATCCTCTATCACGAATTAAATTTCCTATGGCTGCAGAAATATCACCAATGTGTTTGCCAACTTTAATTGCATTAAAGCCGGCATAAAAAGCGTCTTTTGCGCAGGCAATTAATTTTTTGTCTGCTTCAGTTATGTTGCCAACTCCTTTTGTGAATGCTGAATCAGAATAATAGCCATCATAAATTGCTCCAGTGTCAATTTTGACTATATCTCCATCCTTTACAACATAATCACTAGGAATTCCGTGAATTAATTCATTATTAACAGAAATGCAACATGTATTAGGGAAACCATATTGTCCTAAAAACGCTGGTTTTCCACCCCTTTTTCTTATTTCTTTAAAAGCTACGCTATCAATTTCTTTTAAAGAAACGCCTGGTCTTATAAAGTCGTATAAAACAGTTTTCACTTCTGCCAGGATTTTACAAGCTTTTTTGATCTTTTCAATTTCAAATTGATTTTTTATTATGACCATTTTAAAGCCTCTTCTACATCATTATATACTTTATCAATTGTTTGATAACTATTTATCTTTAAAAGTATACCAAGTTCTTGATAAAAATTGATCAAACATTTAGTTTGTTCATTATATATTGATAAACGTTTCTTTATTACTTCTGGTTCGTCATCTGGTCTTTTTGTTACTTTTTCGTGGCATTTAATACACAAATCACCAGGTAATGGTGGAAATGATTGCAAATGATAAATGGTTTTACAATTTAAACAAATTCTACGTTTTGAAAGACGGTCAATAATTTGTTTATCAGTAATTTCTAGTAAAATTACTTTATCAATTTTGATTCCAACATTTGCTAACGAAAGTAGAAACTTTGCTTGAGCTAATGTACGGGGATATCCGTCTAATATGAAGTGTTTATTTTCTCTTACTAATTCAGTTAATTTTTTTTCAACTAGTTGATTTGTTATACAATCATCAACATATGCTCCTGAATCTAAAATAGATTTTATTTTTAAGCCAAGGGGAGTTTGATTTTTAATTTCTTGTCTAAACATATCTCCAGTTGAAATCTGAACATAATCATTATTTTGCACCATTTTTGTTGCAATAGAGCCTTTACCAGCTCCTGGTGCGCCAAGCATAATTATATTAGGAAATTTTTTTGCGTGTTCGCTGTACACTTCTTGAGATTTTTCTATCATATTAAATCTTCTTCTTCCTCTCCATACATTTCTTTTAATTCTCTTATCATTTTTCTCTTTCTTGATAATTCTTGTGACTTGTAGCGGGCTTTAATTTGTTGAACAGTTTCATAAGCCGTTGAAACTAGTATCATTATACTTGTACCGCCAAATGCTATTGAGGCTGGCATGCCTAGCATTTGTTGAATAAATTGTAAAGCGCCTAGCAACAATAAATATCCTGAAGAAAAGAATGATAAACGTAAAATTACATTCAATAAATAATCTTCTGTTTGTTCTCCGGGTCTAACGCCAGGAATAAAAGTACTATTTTTAGCAAAATCTTCACTAATTTTATCAATTCTTGATTGTTGGATTCCCATTAATATTGTTAATCCAAATGTAATTAAAATAAATAGAAAGAATCCAAGTGGTTGAGTAAATTGCAAATTGTTGTAAACTCATTGATAATACTTGCTTGTATTTGGATCAAATAAGTTTGCAATCATTGTTGGCACACTCAATATCATCATTGAGAAGATAACTGACATGATACCAGCAGGATTTGCTTTAATAGGTAAATAACTCAATTCTTTTTCGTTTTTAGAAAGACCCGCACCAACTTGTTGAATTGGAATATGTCTTTCTGCAATTGTAAATATTACAACAATAAATATTACAAGTAAATAGCAAAAGATATATACTGCGTAATTTATTGCTTCTTGTAAAACTAAATTATTTTGTTGAGCTGATGGAACATAAAATTTAAATGCACTCTTGAATGAAGGAATTAAAGCACTTGCTATACCAACAAAGATTATTAAACTCGTTCCATTGCCAACGCCTCTATTAGTTATTTGTTCTGATAAGAACAATGAAAAGAATGAGCCAGCTACTAATATTGCAGGCAAAATGAAATATTGATATGCTACCATTCCACTTTGTCCAAATAATTTTTCTAATTTTGGTGAAAAAGTAATTCCAAATCCTTGACGAGGATTAACTAATGCTTTTGTTATCAAATATGCTTGAACTATTGCAAAAACAAAAGTAACTCCGTAAGTAATGAAATTTATTTTAATCCGGCCTTGAGGACCAGATTGACTTAAACGGTGAATGGCCGGGAATGCTTTAGTTTGCAATATCATCATAACTAAACTAGATGATATAAACGGCCCTATTCCTAGCGCGACAACAGAGAATTGTCTTAATCCACCGCCACCTACTAAATTCAAAATCCCAAAGAAATCTCCTTGGGTTAATGAATCAGGGTTTGCTAAATTGACACCTGGAATTGTAATTGTTCCAGTGGCTAGAAAAAGAGTAATAATGAATAAGGTGAAAAGAATCTTTTTAAAAAGATTATGATTTTTTCATCATTCATTTCAAGTGTTTTTTTTATCAACTAAAAACTTATCTAGTGCTAATTTGCGCTCGATCTTTTCTTGACTAATCTCTTTTTCCAACTTAGTTTTTCTAGCCATTATTTAACCTCTATTTTTCCTCCGACTTTTTCAATAGCTTCAATAGCGGATTGTGAAAGTGTTGGAATTTGTACATTAAGTTTTTTAGTTAAAGAACCCTTAGCTAAAATTTTAACTGGTAATGTTCCTTTAATCAAGTTTCTTGCAGCAAGTGTTTCGTAAGAAACTACTTCACCATCTTGATATTTTGATTGTAAATCTGCTAGATTTACTACTTGGAATTCAACGTGATTTACATTGCTAAATCCACGTTTTGGAATTCTTCTAAATAGTGGCAATTGGCCACCTTCAAATCCTAGTCTTACTTTGCTTCTTTTTGTTTGACCACTTTGTCCTCTACCGGCTTGTTTTCCTTTTCCAGCAGCATGACCACGACCAACACGGTGTTTGTCTTTTCTTGCCCCTGGAGTTGGTTTTAAATTATGTAATTGCATAATCACTCCTTATTTTGATAATAGATGATTTACATCAATATCGCGTAATTTTGCGATTTCTTCAACAGTTCTAACTTTTTTAAGTGCCTTAATAGTAGCTTGGACAATGTTTTGTTTTGTTCTCGAACCATAAGTTTTTGTTGAAATGTCGGTGTAACCTGCTAATTCAACAACAGCACGAACGGTGTTTGAAGCAATGATTCCGGCACCTTTTGGAGCTGGACGTAATTGGATTCTTGATGCTAAGAATTTATCTTGAATTTCATGAGGGATTGTTCCATTTGAAATTGGAACTGAGAAAACATTTTTTTGTGCATCTTTAACTGCTTTTTTAATTGCATCTTGGACTTCATTAGCTTTTCCATGTCCAAATCCAACTTTTCCTTTTTTGTCACCAACAACAACATAAGCTGAGAATGAAAATCTACGTCCACCCTTAACAACAGTTGTAACTCTTGCAATGTCAATAACTTTTTCTTCGAATTGTTTTGCTTGAGCTTGATTTGCTGAATGATCTTGATTACGATTTTGGAATTGTTTTGGTTTATTTTCGCTTGATTTTAGATCTTTTGAATTTGATCTTTCTCTTTTAAAAGAAACAACCTTTGTGCCTTCTGTTTTATTTTCTTTTAATTCAGGTTTTTCTGAATTTTTAATATTTTTTTCTACTTCAGCCATTAGAATTTAATCCCTTCTTCTCTTAGAGTATCAGCAAAAGCTGCTAATTTTCCGTGGTATATGTATCCTGAACGATCAAACACTATTGATTTTACATTGATTGCCAATAATTTTTTAGCAAAATCTTTTGCAACTAATTTAACTGCATCAATATTGTTTGCTTTATTTTCTAGTATTTGTGTTGATGAAGAAGCAATTGTAGTGTGCAATTCATCATTTACTGCTTGTGCATAAAATGCATGTAAAGATTTAAAAACAACAACTCTAGGAACTAATGCAGTTCCTTTTGACAATTTGTTAGTAATCTTTAAGTGTTTTGCTTTACGTTTTAAATTTCTTGATAACATAATTTATAACTCCTTAGGCTACTATTTAGCAGCTGTCTTTCCTTCCTTACGTCTAATAACTTCATCTTTATACATAATACCTTTTCCAGAATATGGACTTGGACGTCTTACATCACGAATATTTGCAGCAAATTCACCAACAGCTTGTTTGTCAATTCCTGAAATAATAATATTGGTTGGTTTTGGTAATTCAATTTTTAGATTTGATGGAACTTCTAAATTTACAGCATGTGAATAACCAGCAATAATTTCAATTGTATTTCCCTTTAAGGTGGCTTTGTAACCTACACCTTTAATTTCAATTTCTTTTTTGTAACCACTTGAAACACCTATTAACATATTTTTAATGTTTGCATTTGTTGTTCCGTGTAATTGTTTTGTAGATTTTTCTTCGTTAGATCTCAATGTCTTAATTTCGTTGTTTTCAACAACTACTGAAATTAATGGTGAGAATGTATAACTCAATTCTCCTAATTTGCCTTTTACTTCGATATGGTTTTTATCTAATTTAACTTCAACATCTTTTGGTATTTCTAAAACTCTTTTTCCAATTCTAGACATAATTACTTCTCTTTAAGTTTATTATCAAATGAAGGCGATAACTTCGCCACCAACATTAGCCTTACGAGCTTCTTTATCTGTTAATAAACCTTTTGATGTTGAAATAATAGCTGTTCCATATCCACTTAAAACTCTAGGCAATTTTTCAGCAGATGAATATACTTTTAAGCCAGGTTTTGAAACTTTTTTAAGACCAGAAATTGCTGATTGATTTTGATTCATACCTTTGTATTTTAAAGTAACTTTTATTTCTTTAAAATTGGTTTTTTCGTTTTTTACAACTTCAAAATCAACAATATATCCTTCTCTTTTAATAATTTCTAAAATTTTTGCTTTCTTGTTTGAATGTGGCAATACAACTTCATGATATTTACGAGAAATTGCATTTTTAATACGTACAAACATATCTGCAACTGGATCTATAATAATAGCCATAATTATCAGCTCGCTTTCTTAACACCAGGAAGTTTTCCTTCATGTGCTAATTTTCTAAAACAAATTCTACAAATTTTATATTTACGCAATACAGCGTGAACACGGCCACAAAGTTGGCAACGAGTATATTTTCTAACCTTAAATTTAGGTTGGCGTTCGGCTTTAACCTTTAATGATGTTCTTGCCATAATATCTCCTAATTATTGCCCTTTGCAAAAGGGATTCCTAAGTGTTTTAATAATGCAAATGCTTCTTTATTGCTTTTTGCAGTTGTTACAAGAATAACATCCATACCTTTTAATTTACGGATTTTATCAAATGAAATTTCAGGGAAAATAATTTCTTCTTTAATGCCGATAGCATAATTTCCACGGCCGTCAAAGCTTTTTAGTGATGTTCCATTGAAGTCACGTACACGAGGTAAAGCAACATTAATTAATTTTGTTAAAAATGATCACATCTTTTCTCTTCTTAGTGTAACTTTCCCTCCCATTGGCATTCCTTCACGTAATTTTCATGAAGCCAATGATTTCTTAGCAGTTGTTTGATATGGTTTTTGACCTGTAATTTGAGCAAGTTCAACCATAACTTCTTCGATTGCCTTTGAGTTTGAAACTTCGTTACCGGCAGTCATGTTAATAACGATTTTTTCTAGTTTAGGAGCTTGCATTACACTGGTGTAACCAAATTCTTTAACTAGCGCGAAACGAATTTCGTCTAAATATTTTTGTTCTAATGCTAATTTAATTTGTTTTGCCATATATTTGCTCCTTTCTAGATAGTCTTATTAATTTTCTTCATCACTCTTTGTTTTTTGTTGGTTTTAGGATCAACTTTTCAACCAATTTTTGAACCGATTGATTTTTGACCTTGAGCACCTTTTTTAACCAAATAAGCAACGTTTGAAATATGAATTGGGTATTCTTTCTTTTCAATTTTTCCATCATTATTTTCTTGTGATGGTTTATGGTGTTTAGTTTTAATGTTTACGCCTTTTAAAACAACAGTTTGTTCTTTTTGATTTGTTGATAAAACAGCAGCAAATTTTCCTTTGTCTTTACCAGCAATAATTAATACCATGTCATTTTTTCTAATTTTAGCTTGAGCCATATTATAAAACCTCCGGAGCTAATGAAACAATCTTTAAAAAACCTTTTTCACGTAATTCTCTTGCTACTGGACCAAATACCCGAGTTCCTCTTAATGAGTTATCTTCCTTAATTAATACAACAGCATTGTCATCAAATCTTATATGAGAACCATTTTCACGCATTAAACCACGTTTTGTTCTAACTATAACCGCTTTAACAACTTGGCCTTCTTTTACAATTCCATTTGGAATAGCTTTTTTAATAGTTACAACAACAATATCACCAATATTGGTTGATCTAACAATTGATCCACCTAGGTTTTTAATAATCATAACTTCTTTTGCGCCTGAATTGTCAGCAACGTTACATCTTGAAAATTCACTAAGCATTTTCTGCTCCTTCAATTGCGTGTGATTTGATTTCTACTAGTCTAAAGTGTTTGGTTTTTGATAAAGGTCTTGTTTCTTGAATTTTAACAATGTCACCAACTTTAGCTAAATTTTGTTCATCATGAACTGCAAATTTCTTTGATTTTTTAAAACGTTTTGAATAAAGAGGGTGTTTTTCGTAAATTTCTACAACAACAGTAATTGTTTTTTGGTTTTTAGTTGATACAACTGTTCCAACTAAAGTTTTACGACGGTTTTCTCTAACTGTAGTTTCCATTATTTATTCCCTTCTAAAGTTTTTGCTTTAATTGCTGTTAAAGTTCTTGCGATATCTTTTTTAACTAATTGGATTTTGTGGGTTTCGTCTAATTGTCTTGTAGCATTTTTGAAACGTAATGAGAATAGTTCTGCTCTTAAATCAGCAACCAACTTATTTAGTTCGTCAAGTGATTTTTCTTTTAATTCTGCAAATGTCATAGTAATTATTCTCCTTCAACTTCATTAGTTTTAGTTTCTAAAGATTGTTCTTTTGAAATAACTTTTCAAGTTACAGGTAGTTTGTGACCACCTAATCTCAATGCTTCCTTCATGGTATCTACTGTATTGCCTAAAACTTCAAACATTACTGTTCCTTCTTTTACGACTGCAAATCATTGATCTGGGCTACCCTTACCAGATCCCATACGAACACCAATAGGTTTACTAGTTTTTGACATGTGAGGGAATATTTTAATAATAACTTTTCCTTCACGTCCCATATGTCTAGTAATAGCAATACGGGCTGCTTCGATTTGTCTTGCACTAATTCATGCTGATGAAGTTGATTTTAAACCAAATTCGCCAAATGCAACTGTATTATTACGGTGTGCAACAACTTTGTCGTGGCGGATACGGAACATTTTTCTATGTTTAGTTCTTTTTGGTTGAAGCATGTCTTTCACCGCCTTTTTTAAATTCTCTTTTACGTGGAGCTTCTTTTACTTCTGCTTCACTTGTTTTAGGTGATAAAATTTCGCCTAATGAAACTCATACCTTAACTCCTAAGATACCATATGTTGTTAAGGCTGTTGTTACAGCATAATCAACATCTTGTCTTAAAGTATGTAATTTCATTTCACCTTCGGTATATCCTTCTGTTCTAGCCATATCAACGCCATTTAGACGTCCAGCTACTGAAGTTTTAATACCTTTTGCGCCAGCTTTTAATGCTGCTTTAATTGCAAATTTTTGTGCTAGTCTAAAACTACCACGATTTTCTAATTTAATTGCAATTCCTTCTGCTAGCAATCTAGCATTTAAATCAGGATTTTTTAATTCGATTACTTCAATAGTAATGTCAGCTTGTTTTTTAGCAATAACCTTTTTTAATTGTGCTGTTAAGGCTTTGACATTTTCACCATTTGTTCCAAGGAATGCTGCAGGTTTTGCAGTGTGAATATAAACAATAGTATGTCCTGCTTGATCACGTTTAATTTGTACTTTACCAATTAAATATTCACGAACTTTTTTGTCAAAGAAATCATAAATTTTTTGATCTTCTAATAAATAAGTTGCAAATTTATCTTTATCTGCATATCAAGTTGTGTTATGAGATTTTGTAATTCCATAACGAAATCCATTTGGATTAACTTTTTGACCCATTATTTATCTCCTTTTTTGTTGATTTCTTCAACATTATTGATTTCTGTTAATTCAATAAAGAAATGACTTGTTCTTTTTAAAATTGCATAAGCACGTCCACGTGAGTGAGGTTGGAATCTTTTTAGTGTAGGTCCATCATTTACTAAAATGGTAGTTACATAAAGTTTAGTTGCATCAAGTCCAGCATTATTTGTTGCATTAGCAATTGCTGAATTTAGAAGTTTTAGAATAATTTCTGACGCTTTTTTGTGTGTATGTTTTAGAATAACTATTGCTTGAGTTGCTGATTTATATCTAATTAAATCTGCTACTAATCTTGCTTTACGTGGTGAAATTCTTTGCATGCGAACTGATGCATGTGCTGAATTTTGTAAAATTTCTTTAGCCATATTCTATTTCTTTCCTTTATCTGCACCATGACCATTGAAGGTTCTTGTTGGAGCAAATTCACCTAGTTTGTGTCCTACCATATCATTTGTAACAAATACATCAATAAATGCATGTCCATTGTGAACTTGGAAGGTTAAACCAATAAATTCTGGATAAATTGTTGAGCGTCTTGATCATGTTTTAATTGGACGTTTTGGTGATTTATTTTCAATAATAGCCAAAACTTTCTTCATTAAATGATCATCAACAAAGGGAGCTTTCTTTAATGAACGAGCCATTATTTAGTTTCCTTTCTTCTTCTAATAATAAATTGATTTGATGCTTTTTTAGTTGCACGAGTTTTAACGCCAAGTGCTCTTTTTCCTCATGGAGTCATAGGACTCTTTCTACCAATAGGTTGTCTACCTTCACCACCACCATGTGGGTGATCATTAGGGTTCATAGCTGAACCACGAACTGTTGGTCTAATTCCTCTTAAACGGTTACGTCCTGCCTTACCAATATTAACAAGTGAGTGTTCTTCGTTTCCTACTTCACCAACTGTTGCACGATTAATTGCTAAAACTTTTCTAACTTCGCCTGATTTTAATCTTAGAATTACATAACGACCTGATTCATCTTTACCTAAGATTTGTGCTGATGATCCAGCTGATCTAGCAATGATTCCACCTTGTCTTGGTTGTAATTCAATATTGTGAACAAATGTACCTTCAGGAATGTTGGCTAATGGTAAATGGTTACCGACTTGAATGTCAACATTATCACCTGACAAAATAACTTGTCCAATTTTAATACCTTTAGGGCTTAAAATATATCTTTTTTCACCATCTCTATAAACAACTAATGAAATATTAGCTGAACGATTAGGGTCATATTCAATAGTTTTTACAGTAGCTGGAATTCCATCTTTTTGACGTTTAAAATCAATGATACGATAGAATCTTTTCAATCTACCACCTTGATGACGGGTAGTGATTTTACCTTGATTGTTTCTTCCTGCTTTTGTTGGAAGAGCAACAAGCAATGATTTTTCAGGAGCATGTCCTGTTAAATTTGCTTGATAATCAAGAGAAGACATATTACGACGGCCATTGGTATATGCTTTAAATTTTTTAATAGCCATGTTGTTTTCTCCGTTCTTAAAAATATTGTTATCTTTTAAGAAAATATTAATTATTTTTTTTAATTTAGTTTCATCTTATATTTATGCAAGATTAACTATTTGTTTTCAGCAGCTTTTTTAGCTTCAGCTGCTTTAATTTTTTCTGCTGCTTTTAATTCTGCAGCGGTAGGTTCAGCAGGTTTTTCTTCTTTAACTTCTTTTTCTGCTGTTGCTTTTTCAGCTTCTTTGTTAGCTTTCTTTAATTCTTCAGCAAATAGAATAATTTTTGAATCTTTTGCTAAATATACAGTTGCTTTTTTAACTGAATTTTTAAAACCTGAGAAACGTCCTAATTTAGCAGGTTTTTTATCGTAATTAGTAATGTTAACTTTTAATACTTTTACATCAAAAATAAATTCAACTGCTTTTTTAACTTCGATTTTGTTTGTTCTTTTATCAACTACAAATGTATAAACGTTTTTATTTTCACGTGATAATTCACTTTTTTCTGTTAAAAGTGGGTATTTAATAACTTCATTTATATTCATTATTTAACCAATCCTTCTAAGTATTTAACATCATCTGAACTAATAACTAAAACATCTGCTGCCAATAAAGATTCAACTGATAAACTAGTTACTTTTGTTACAGTTACATTTGGTAAGTTTCTTGCTGATAAGAACACTGTTTCATTATTTGAAACTAATAAAACCTTGTTTAGACTAGCAATTTTATCTTCGTTTAATTTAATTAATAATTCACGAGTTGAAATTTTTGATAAAGAATATTCTTTTACTAAAACAGCATGGTCTTTTGCTAATAAAGTTAATGCTGATAATAAAGCGTTTCTTCTTGTTTTTTTGTTGATTTTTAGATTGTAGTTTCTTTCAGTTGTTGGGCCGAATACCTTACCACCACCAACAAATATAGGTGATCTTAGTGATCCAGCACGAGCATTACCAGTATGTTTTTGATCTCATGGTTTCTTACCACTTCCACTAACTTCTCCACGAGTTTTAGTTTTGTGAGTAGCAAGTCTTCTTGAGCCACGTTCTGATAAAATTGCATCAAAAATTGCTTGGTTATAAATTTTTTCTAAACCAAATACTGATTTAGGAAGATTTTCACTTGAAAATTCAACGTGTTCTTCATTATCTTTTTTAACTGATATTTTTGATGGTTTTTTAGGAAGAGTTTTTTCTACTAGATTTTCTTTTTCTAAGTAGTCGATTGCTTCTTCATTTTTAACTTTTTCGCCAGTTACTTTTTCTAAAATTAATTTAGCTTTTTCTAATCCAACTGATCCGCGGTAAGCGCCATCACGGTGGAATCATACTCTAGTATTGTTTTTTGATTTTTCAGAAAGTTCAATAAATTCTGTTAATGCTTCTGAAAAATTTTCAAAATTCTTGAATGATTTTTGGTTAGCCTTTTTTAAATTAAATGAAATATTACGATTTTCATCGAATTTTTCAGAAATGTAGAATTTTTCTAATGTTACATTTTTTGTTGCCATTATTTATTTTCTCCTTCTTCTCCGGCCTTAGCAATTGCTTCATCCAAAGCAGCTTTCATTTCTTTTAATGACATATCCATGTTTAATTCAAGATTGTATTTTTTAGCTTGAGTAAAGATTTCATTTTTAGCCAAAGCTTCTTTTAGATTCAATAATTTAATAACTGGTTTATTCTTAATTGATTTCTTAGCAGTTTTAATGATTAAGAATGCTCCTTTAGCACCAGGAACTGATCCCTTGATCAATAAAATATTGTTTTCTGCATCTTCTTTAACAACTTCTAGGTTTTGAACAGTTACTTGTTCAGCTCCTAAATGTCCTGGCATGGTCATACCTTTTCAAACCCTATTTCCAGAAATATCTCCTAGAGAACCGGTTTGTCTTACAGGTTGTGAACCACCACCACCACCGTGTGATTTAGGGCCAATATGTTGATTTCATCTCTTGATGGTACCCGCAAATCCTTTACCTTTAGAAATAGCTGTTACGTCAACAACTTCTCCAGCGGTAAATAATGAAGCATCTACAGTATCACCTAATGAAAATCCTGACATATCACGGATTTCTTTTACGAAGCGCTTAGGTGTTGTATTTGCTTGTTTAAAGTAATTAACTTCAGGTTTAATGATTCTTGAAGATTTTTTATCTTCTGTTGCTAATTGAAGAGCAACATAACCATTTTTTTCTGATGTTAAAACCTTAGTAACAACATTTGGTTTAACTTCTACTACTGTCACAGGTATTAATTTACCTTCTGAAGTAAATAGTTGAGTCATCCCAACTTTTCTTCCTAAGATTCCTTTCATGATTATCCTTTCAAATTTATTAATTTATTAAATATATATACTATATTTATAAATAAAAAATAATTACTGTTATTAGTTAACAGTTTTTAAACTTATTTGTACACCAGCAGATAAATCTAATCTCTTAGCCTTATCAACTAATGATTCAGAAACTTTATCTAAAACGATTAAACGTTTATGAGTTCTGCTTTCAAATTGTTCACGAGATTTCTTGTTAACGTGAACAGATCTTAAGATGGTAATAATTTCTTTATGTGTTGGTAAAGGAATTGGACCACTAATAGTTGCTTTTTCAGCTTGAGCTAATAAAACTATTTTTTTAGCAGCTTCATCTACTAAGTGGGCATCAAAAGATTTAATTTTGACTTCTAGTTTCATATTTGTTCTCCTTCTTCGTAATTCGAAGTAACTCCACACAAGTTCTCAATATGCGAGATGAACAACTAAGCTCGGCATATCGTAACCTCGTGTTTCTTCGTTGTAGTTGCAAAAGAATTATAACAAAATTTTTATTTTTTAAAAAAATAATTTTTTTTGATGAAAAAAACATGCTTTTTACTTTTGATTTTATTAAAAAATTAACGATAAAAAATAATCGACTTATACTAAAAAATATTGATATTAAAAAACAATATAAATAATTAGTTTCTTATAAAAAATATTAAAAATAGTTGTGTAAGGTCAATAAGTATATCATAAGGTCTATAAAAAGCTACGTTGGCTCTGTTTTATATCACTAAAAGAATTTGTTATCTGTAAGTATAATAGTTCTTTTTTGTTTTTAAGTTTTTTATAAATAAATCAAACTATGCTATAATACATTCATATGAACCTAGAGAGATACACGTAAGTAATCGAACAGTAGCGTGCTTGACGTGAAGCTACAAATACTCTAGGCTGGGTCGTCTTAAAAAGAGGTTTAGCCTCTTTTTTTGTATAGTTTTTATATATGTTAGAGCATGAAAACCATTAAAAATTACAAATCCTTTATTAAAAGGTTTTTTTATCAACCAATTGATGGCAAAGTTTTAAATAATGTAAAAATTAAAGCTCGCCCACTGATAACCTTTGAGAATGTTACAAAATTAAATTTATTTGTAGTATAATTTTTGTAACTTAAGAAAGGATATTTATAGGTAATCGAATAGTAGGCAGCCTATAAATTTTAATTTTTTGTAAGGCCTAATGATATGTCTTAAGTCTCGGTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATAATTTTTAAACGTTTTTACATCTTATTTAATTTATTTATGATAAAATTTAATCGAATTTAGGGCAATACACGTATGCAATCGAACAGTAGCGTGCTAGTTCGTGTAGCTACATTCTCCCTAAACTGGTTCGTCTTAAAAAGAGGTTTATACCTCTTTTTTTGTATAGTTTTTATATGAATTTAAAACTATGAAAACCATTAAAAATTACTAACTCTTTATTAGAAGATATTTTTAAGTTGCCAATTGATGACAAAGTTTTAAATAATAAAAAAATTCAAGCTCACCCCCTTATAATCTTTGAAAATATTATAAAATTAAATCGTTTTACGATATAATGAATTCATATGAACCTAGAGAGATGCATATAAGTAATCGAACAGTAGCAAGCTTATATGTAGCTACAATGCTCTAGGCTGGGTCGTCTTAAAAAGAGGTTTGTTCCTCTTTTTTTGTATAGTTTTTATGTGAGTTTAGAATTATGAAAACCTTTAAAAATTGTTAACTCCTTATTAGAATATTTTTGGATTGTTTGTTAATATCAAAATATTGCCGAATAAGAAATTTAAAGTCATTCCTTACTAAATTTTAATAAGCATTTTATTAAAAAACAAATTTATTTATATTAAAAACAATCCTCGAATAAACAAGTAATCTACTTACATTATTTAATATTATCGCTAATATGTATTCACCATTGTATTTTAGACAGAAGAAGACAAAATCTATAAAATTTTTTGTTTGGTTTTATAATTAAAATATGCTTAAAATAAATAAAAAATAGGTACAAAAAGGTAAATAAATAATAAAGAATAAAAATTGTCATTATAAGACTTAAAAAATATATAAATAATTAAGGAAGTATATGAAGAAATCAAAAAGAATTTTATTAACTATAGGGGCAGTAATACCTTTATTAGCCACAATACCATTAGTTTCATCAGGTTGCATAGAAACAAAAAAACCCGAAGTTAAACCAAATGAGCCAAAGAAACCAGAAAATAAAACATCGGGTTCAGATTCAGGTTTAATAAATCCTAATCCAAATCCTTCAAATCCAACTCCAGCTCCAATTCCTATTCCAGAAACTAAACCAGAAGAAAAAGAAGGTTATGTTGTTTATAAATCAACAAATGAAGATTATTTAAAAAAGAATAACATTTATTACTTTAGCAAATTACTTTATGACCCTGTGGATTTATCTGATGAGGATTATAACAATCATGAATGAACACGTTGAAGACCTAAAATGTGGTTTTCTTTAACTAATAGAATAGAATTAGAAGAGCAATTAACCAAATGAAGTATGGTTTTAACTGATGAACATTTTACAAATAACTGTGAAAGTGCTTGTGGCATTTGAAATATTATTAGAAAATTAGCAGAAAGTGATGAAAACAAAGAAGGTATCAACCAAGGCGGTCGTTTTAAACCAAAAGATAATGAAAGTTATAATGAAGCCTTAATGAGATTTTTAGATGAAATGGATGATTATTTAGATCCTGATATTGCATTTAAAGGTTTAACAATGGATGAAACTCATATAAATTATGTAACGGAAAATTTAAAAAAAGATCATGATTGAATGAATAAAAAACTTATTTACTCTGCTGATTCAAAAATGCCTTCTACTTTTATTAGAGACGAATTTAACCACCAATCAATTGAAGACATGGATGGATTTGTTAAAGAATTAATATCTGATTATGAAAATGCTATGTATGAAGGATGAACCGTTGAAAATAAAGCAAATGATTGGTTTGTTGCTCCTTTTTCATTTGGATCTAACCCTACAATGAGAAATAAATTTCCACAAGAAACTTACAATATGTACACTGATAAAAATGGTCTAGATAAAACAACTCCTACTCCTGCTTATAATAACTACAATCCAGTAGCATTTAATTTCTTAGTAATGGAAAGATTTGACTTATCAGAAAGATTAAATTTTGAAGTTTATAAGAATGTAATTCAATGACTAAAATCATTTAATAATATAAACTCTGAAAAAAAATCAAAATGAAGTGAAATCGAAAGTGATAAAAAAAGAAAATTAATGTACGCTTCAACTAATTTAGTAAGGGCGTTAGTTAAATTTATTGATTGACATCATTTACTACTAGGAGAATTTGAAAATCCCGATGATAAACATTCTACTTTAGCTGATTTGTTTGCAATGAAGAGTTTAACAATGGGCGACAAAATTGACTTTGATTACACTTATAGATTTGCATATAAATTTTTATATGAAAAATTAGTGCCTACAATGGTGGCAAACGGTTGAAACATGCCAACTGAATTTGATATTAATCTAGAAAGGAGTATGCTAGATAAAAATAGAAAAGATTCATATATTTATGACTATATCTTTAAATATTTAAATGTCATTTTTAAAGCTAAATACAAAGACATTGATGAAATTAAAAAACCAAAAGGATTTTTAGATTTAAATAATAAAGAACATTGACAAAAATTGTTTTTAGACAATGTTTTAAAAAAATGAAATTCAGCGTTTAATATAAATGTTGATTTAGAAGAATTTTAACTTATATTAGGTGTTTAAAACACCACAACTTATTTTTAATTTTGAATGATAAAAAACAAGCAATTTGATTTTCAAATTGTTTTTTTCTATTTATTTTTTTAATTTTTAAAATTAATCCTATTAATCAGCATCCGAAAGATCTAAGTAAATATATCCTTCTGATTCTTTACCCATTAAATTGTTTTTAATCATATCTGGACAAAGAATTTGTATATAAATACAAGGCATATTCTTTTCTTCACCTTTTTTTAAAAATTCAATTAAAAGTTTGTGACCTCAAGGCATAAGATTTTCAGGAATTTTTATATCAAAATATTTTTTAATGTCATTAGCTTCCATAAAATATGTATAAGCAATTCTTTCTTGACTTTTTAACATTTCAATAGTATCGTATTTTTTAGTTTCATCAATTGCTTTATTAACAATTTCAATTAAGGCTTTGGCTCCATTATTGGCTACAAATTTTTTGCCTTCTTTGGAAAGTTCAAATGAAAATTGTGTTGTTATTTCTGAAATTTGAGAAGCGGATAATAGCTTTCTTTGAAACATTCTATGAAGTCTATCGGGTTGTACGGGATCTGTATCATCAGTATTTTCGTTATTTGTATCTTTATTGTCATCTTTCTTGTCGTTAGTATCGGCGGTACTGGTATTTGTGCTGTCTGTATTTTTGTCATTAGTATCTGCAGTTCCAACACTAGAATTACCTATATTCTTATTATCAGTATCAGTAGTACCAGTATTTGTGCTATCTGTATTTTTGTCATTAGTACCTGTAGTGCCACTATTATCTATATCAATAGTGTTATTACTTGTATCTAAGGTATTATTAATTGTGGTAGTTTCCTTGCAAGATATGCTTAAAAGAGGTAATAATGACACAGCTGGAAAAATAAAACTTAATAAAATATTTTTTTTCATATTCCCTTCACCTTTTAATAACTATGATTATACAACAATACGGCAAAATATATTAGTCAAAAAAATTTTAAATTTGAGCAAAATAATGTTTATATTTCTTTACATTTAGTAAAATTTAATTACTAGATAACAATATTTTGTTTTTTAGTTTAAATTTCAAATAATAAAATAATAATAAAAATTAAATCAAGGAGAAATATGATTTCTAAAATTTATGATGATAAAAAATATCTTGAAAAAATGGATAAATGATTTAGAGCTGCTAATTATTTAGGTGTTTGTCAAATGTATTTAAGAGACAACCCTTTGTTAAAAAAGCCTTTAACATCAAATGATATTAAACTATATCCTATTGGACACTGAGGAACAGTTCCTGGACAAAACTTTATTTATACACATTTAAATAGAGTAATTAAAAAGTATGACTTAAATATGTTTTATATTGAAGGTCCCGGACATGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCTGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCACCCGAAACACCTGGTTCAATTCACGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGTGAAGCAGAAACAGGCCCACTAGCAACTAGCTGATTCTCAAATGCTTTTATTAACCCTGTAAATGATGGAGCAATACTTCCTATATTACATTTGAATGGTGGAAAAATTTCAAACCCTACACTTCTTTCAAGAAAACCAAAAGAAGAAATTAAAAAATACTTTGAAGGTTTAGGCTGAAATCCAATTTTCGTTGAATGAAGCGAAGATAAAAGTAATCTTGACATGCATGAATTAATGGCAAAATCATTAGATAAGGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCTGCTGAAGAAGCAACAAGACCAACTTGACCTATGATTGTTTTAAGAACTCCTAAGGGATGAACTGGACCAAAGCAATGAAACAATGAAGCTATTGAAGGAAGCTTTAGAGCTCACCAAGTTCCTATTCCTGTTTCAGCATTCAAGATGGAAAAAATTGCTGACTTAGAAAAATGATTAAAATCATATAAACCAGAAGAATTATTTGATGAAAACGGAACTATAATAAAAGAAATCAGAGATCTTGCACCAGAAGGTCTAAAAAGAATGGCTGTTAATCCAATTACAAATGGTGGAATCGATAGCAAACCTTTAAAATTACAAGATTGAAAAAAATATGCTTTAAAAATTGACTATCCTGGAGAAATAAAAGCCCAAGATATGGCAGAAATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGACTAGAACCAGTTTCTAAGAAATACGATGAATGAATTTCACCTGCAGGTAGAATTATTGACTCGCAATTAAGTGAACACCAATGTGAAGGCTTCCTAGAAGGTTACGTATTAACCGGAAGACATGGTTTCTTTGCATCTTATGAAGCATTTTTGAGAGTTGTTGATTCAATGCTTACTCAACATATGAAATGAATTAAGAAAGCTTCAGAATTATCATGAAGAAAAACCTACCCTTCATTAAATATTATTGCAACTTCAAATGCTTTCCAACAAGACCACAATGGTTACACTCACCAAGACCCAGGACTTTTAGGACACTTAGCTGATAAAAGACCAGAAATTATTAGAGAATACTTACCAGCTGATACAAACAGTTTACTAGCAGTAATGAATAAAGCCTTAACCGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGCTGAGGATGCCGAAGAATTACTAGAAAAAGGTTATAAAGTTGTTCCTTGAGCTTCAAATATTTCAGAAAATGAAGAACCAGATATCGTATTTGCTTCAAGTGGTGTTGAACCAAACATTGAATCATTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTTTATGTTCTTGACTTATTAAAATTGAGATCTAGAAAAATAGATCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGATTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGTGATATTACAACATCATTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAATTAGACCAAAAACTAGAATATCATAGAAACTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGAAAATGAAAGAACATAAACAAAGAAAATTAATTTTAGTTTAAATAATTTCTTATATTTATGGTAAAATTGACTAAGTTAATTAACGAAAAATAACATAGGAGTACACATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAATTAATTAGCATTAATAAATAGGCGAAATGCCTATTTTTTTATTATGTGCAATAATGCATACCAACTGCATAACCAATGTTTTCATTGCTATTGTCTCATTTCATTGAAATATTCCCGGCTTTTACTTCTCCTTTATTTGCATTTAAAGTCTTAGCCAAGCCTATTGAAGTTCCAATAATTCCGACAGCGCTTGTTACTACAGACAAAATAGTTGAAATTAAAGCAGTAATTGCAAAACCTCCTACAATATTTTTTGTTTCTTCTTCGCTCATTAGTTTGAAATTTATATTCATTTTTATTTCTCCCTTCAATTTCAAATATAACAAATATTCCATTACGCTGAATGTATTGAAGAAAAAATAGCGAAAAGTAACAATCTTTTTCGCTATTAGTAATTTAAAATTGTTATGCTCTTGTTATTCGCTAATATTGCTAATAAATCTAAAAATATTGCATATTTAATCTCGTTGAGTTTTCAATAAATGTTATATTCATTCTTTATCACAATTTTCTTTAGTAATACTTATTTCTTATTTGATAAACAAAATTTAAGGCCAATATTATTTATACACTATATTTAGAATTATTAGTTCTTCAATAATTATTATTGCATTGACTTTGATAAATAACTTTGTAAACTATTTTGTAACCTTTTCAGTATATTCATTACTAATATTAATATGTAGAATCTTTGCGAACGAAAAATTAATTTAAAAATTAAGCAAGTTAAAGAACAAAAAAAATACATTTTAAAATTCTATGAATAAAAAATTAGACTCATATAACTTAAGGGTTGCGAGTCTAATTTTTATTTTGTATTTTTATTATTATTTTGTTGAATCAAAAGTATTTTCACTATCAACGTTTTTATCATTGGCTAGTTGTTCTTCTTTACTTTTATTTTGTTTATTATGAAAGCATAAACCTATTATTATTGCAGAAATTATTGTTCCAATTCCTCCACAAATTAAAAATGAATCTGCGAAATTAAATGTTCCTTTATCAATTCATGGAAAATATATAATATCTCTTACACCTTGAAACTGAAATCTATCATACATATTTCCAAGCGAACCTGCTGCTGTAACTGCTAGTCCTGCAACTACAAAAATATATCAATGATTTTTAAAAAATAAACTGCCAATGAAAGTTGCAAAAATTATAATAAAACTTATTATGTGTAATCCAACGTTGCCCAATCCTAATTCCAAAGTAGTTCCAGCATGGAATACAGTTCTATATCATAAAAATGCATGGGCTCCGGGTTGTCCAAATGAATAAATAGCTTCAGAATCTCTATAATCTGCCGGAATATAATAATTTCTTTTAGTTAATAAATCAATTAAAGCAAATATTATGAATATTCCTATATAAACAGAAGCATTTATTAAAGCATTTTTTCATTGGCTCTTCCAATATTCTTTAGTAAAAATGGAAATTTTTTTATTTTGCATCAATTACATCCTTGCATCTTAAACAAATTTGCAATTCATCATCATAATCTGAATCTTCAAAATGGTTTCAACATCTTAAACATTTTTTGGTTTCTAATTCTCTTACTTCAATAGTATTGCCAAATGTTACTTTTGCCACCATTAATAATCTATTTAATGGCAATGATTTTATAAAGTCTGAATCGCTTTTTATTACTACTCATGCTTCATTTGGTCTCTTTATTGCTTGCGATTTAATTTTTTCTTCAATTAATTTGTATACTTCATCTTTTATTTCAAAGAATTCTTTTCATTGATCTTGTAATTTAAAATCAAAATCTTTTCTTTCTAAAAATGACAATAGATGAACAGAAGGCAACTTGTTAGGTTTATTGTAATATTTATAAATTTCTTCGGTTGTAGTTGGCAATATTGGGGCAAGAGCTTTTAATAAGACCTCAATAATGTTGCTAAAAACATATTGAACCATTCTACGTTCAGGATCATCTTGTTTTTCAAGATATAAAATATCTTTGGTTATAGAAATATAGTAACTACTTAAATCAATAACAAAATTGTTAATATCTTTAATAACATTAACAAATCTATATTGTTCGTAATTCAATAATATATTTACTTCTAGATTATGCAATTTTTCTAACATTAAAGCATGAATTGAAGTTGGCGTAATTTCTTTAAAATCAAAGTCACTAATTCCACCAAGCATGAACTTAATTGTATTTCTAACTTTACGGTAAATTTCAGTATTTTGGTTTAATATCTTGTCGTCAATTGTAACATCAGCAGTATATTCAGAATTTGCTGCTCAAAGTCTTAAAATATCTGCTCCATATTTATCAATTACAGCTAAAGGATCAACAACATTGCCTTTTGATTTTGACATTTTTTGACCTTTGCCATCTAAAACAAACCCATGAGAAACCAAAGCTTTAAATGGACTAGTATTTCTTCATGCCACTGAGTTAATTAATGAACTATTGAATCAACCACGATATTGATCTGAACCTTCTAAATAAAGATCAAATGGAGCTTTTATTCCGCCTGGTTCAACACTTATTGAAGTTGATCCCGAGTCAAATCAAACATCCATAATGTCTGTTTCTTTTTTAAGACCTAAATTTCTAAATTTTTCTGGCAATAATTCATCAGCTGTTCGTTCGTATCAAACATCGCTGCCATGTTCTTTAACTAGATTAATTACATGTTCAAAAATTTCATCATCTAGAACAGGATTTTTGTCTTTATCATAAAAAATAATTATTGGAACTCCCCAAGATCTTTGACGAGAAATGCATCAACTTTCACGATTTTCCAACATTAAACTTAAACGTTTTTTGCTTCATTCATTGAATGTTTTAACTTCACTTAATGCCTTAATAATTTGTTGTTTTATTGGTTTTAATGAAACAAATCATTGTGGCGTTGCTCTATACATAATTGGCAAATGAGTTCTTCAATCATGAGGATATGAGTGTTTAATTTTCTTAAACGCAATTAATTGATTATTTTCTGCTAAAAATTTGCCAATTAAAGGATTTGCATCATCGTAAAAAACACCGCTATATTCAAAGCCTTTATCATTGATTGTTCCATCATCGTTTATGTGCATAATCATTTCTAGGTTTTCTTTTTTGCCAATAATAAAGTCATCTTCACCAAATAATGGGGCAATGTGAACTAAACCTGTACCAGTATCTAAACTAACATGATGACCAAAAACAACTGGCCCCACTAATGGGGTTATAGGATTTTTATATTTAATTCCTAAAAATTCTTTACCTTTGAATGTTGATAGAACTTGATATTCTTCTCATTTAGCAACTGCAGCAACTGATTCAACTAAATCTTTTGCAATTATATATTTATTGCCATTTACTAACACTTTTGCATATTCGAATTGATCGTTAATTGCAACGCCAGAGTTAGCCAACAATGTTCATGGAGTCGTTGTTCAAATAATTAAATAATCTCCATTTTCAACGTATTTGTTTCCTTCGATCACTTTGAATGCAACATATAGAGAAGGTGAAATGTGATCAGCATATTCGACTTCTGCTTCAGCGAGTGCAGATTGAGAAGAAGGTGATCAATAAACTGGTTTTAAATCGCGATAAATTAAGCCTTCTTTAACCATTTTAGCAAAAAGTTCTAATTGTTTAAATTCTAGGTTTTTATCCAATGTCACATAAATTTCTGAAAAGTCTGTCAATAAACTTAATTTTTTAAATTGAGCCTTTTGATGTTCAACTTGACTTAAAGCATATTCTGCTGCCTTCTTCCTTAATTCAATAACACTAAAATCTTTAGCGTTCTTTTTAGCTTCTAGCAACATTTTATGTTCAATAGGCAAACCATGTGTATCTCAACCCGGAACATATGGAGAATAATATCCCTGCATACTTCTAAATCTTACAATTATATCTTTTAAAATTTTGTTTAGAGCGTGTCCAACATGAATATTGCCATTTGCATATGGAGGACCATCATGTAATACAAATGATTGACTGTTCTTATTTCTATTTAAAATAGTTTGATACAACTTAATATCTAGCCATTGTTTTTGATATTCCAATTCTTTTTCAACTAAATTTGCTTTCATTGGAAAATCAGTATTTGGCATCAATAACGTATCTTTATAATCTTTCTTTTCTTCCATTATTAACAAACCTTTCTTTTTCTATTTAATTATGAAATTTAAAATTTTAAAATCTTTATATATTACTTTAACTATTTCATGACCTTGTAATCATTTAGCAACATTGTCTAGTTTCTTTGCATTTTCAATAATTTCTTCATTGCTTCAATTTGGCAATATTTCAATTTGAGAACGAACTTTTCCGTTAATTTGAATACCAATTTGTATATTTTTAATAATTATCTTGGTTTCATCAGCATAAGGTCAAGTTTGATATTGAATTGGTTTTTCATTCAACATAAACAAAAGTTCTTCAGCTAAAAATGGCGCAAACGGACTTAATAAAATAGCAAATTGTTTCAAAACAAATGATGATGGAATAGTTTTTAAAGATGTTAAGTAATTAGTAAACACCATCATTTTGCTTATTGCAATATTAAACTTAGTTTTTTCAATATTTTCGCTAACTTCTAAAATCAATTTATTAATGTTGCTTATTAATTCGTTATTACTATCGTTTATATCAATATTTCCTTTGACAAAATATTCAAATATATTGTAGATTTTTTCAATTCATCTTCTTATACCATTTAATGAATCTGTACTTCAAGATTTTGATTCCACCAAAGGACCCATAAACATTTCATAAACTCTTAAACTATCTGCTCCATGAGATAAAATAAAATCATCAGGATTTATAACGTTGCCTAGAGATTTAGACATTTTTTGACCATCAGGTCCCATAATTAATCCTTGGTTTATTAGCTTTGTAAATGGTTCTTTTGTATTAACAATACCCAAATCAAATAAAAATCTATGTCAAAATCTTGCATACAATAAATGCAAAACAGCATGTTCTTGCCCACCAATATACAAATCAACCGGCATTCATTCTTCAAAACGTTTTTTTGCTTCCAATGAATTTAGTGGAGTATATGAGCCATCTGGGTTTTTTAATATATAAGCCAAATAATATCAACTTGAGCCAGCCCATTGCGGCATAACATTTGTGTCATGATGATATTTTTTACCATTAATTTCAACATTCATTCAGCTTTCAACATTTGCCAGCGGACCAGAGGTTGTACCTGATGGTTTTATGTTTTTTATCTTTGGCAATTCAACTAATTCTTTTACTAGATAAATATTATTTTCTTCATCAAATAGAACAGGAAATGGTTCTCCCCAATATCTTTGCCTTGAAAAAATCCAATCGCGTAATTTATAACTTACCTTTTCATCTGCTAAATTATTTGCTTTCAATTCATTGAAAATACATTTTGTTGCGCCTGCATTATCTAAACCATTTGCAATATCTGAATTGATATGAATACCATCTCCTAAAAACGGTAATTCTTCACATTCAATAATTTTTTTAATTTTTAAATCAAATTTTTTAGCAAATTCATAATCTCTTTGATCATGGCCGGGCACACACATTACTGCTCCACTTCCATATGACATCAAAACGTAATCAGAAACTCAGATAGGAATTAATTCATTAGTCAATGGATGAATTGCATACGAACCAGTAAAATATCCTGTTTTGTCTTTATTTAAAGATGTTCTTTCTAATTCATTTTTTAATTTAACTTCTTTTAAATAGTTAGAAATATCATCATGATATTTTGTAGTAGTTATTTTTTGAAGAAGTGGGTGTTCGGGAGAAATTACTATTGCAGTTACACCATAGATAGTATCCAATCTAGTAGTAAATGTTGAGAGTTGCAATTTTTGTTGTTCACTTGAACTAATATTTCATTTGACAATATGTCCTTTACTTCTTCCAATTCAATTTCTTTGCAATTGTTTTAAACTTTCAGGTCAATCTAAATCTTCTAGGCCTTCAAGTAATTTATCTGCATAGGCTGTTATTTTTAACACTCATTGTTTCATAGGCTTTTTAACGACAGGAAAATTACCCCGTTCGCTAACTTTAATACCGTTTTTTTCTAAAATTTCTTCATTAGCTAAAACAGTGCCTAGCCCTTCGCATCAATTAACATCAATTTCTTCAATTGAAGCTAGTCCTTGTTTATACATTTCTTTGAATATTCATTGAGTTCAAATATAAAATTTAGGATCAGTAGTATTTACTTCCTTATCATAATCATAAGAAAATCCCATTGCTTGCAATTGTCTTCTAAAATTAGCAATATTTTTTAATGTAAAAGGTTCAGGATCATTTCCTGTTTTTATTGCATATTGTTCAGCAGGTAAGCCAAAAGCATCTCAGCCAATAGGATGCAACACATCAAAACCATTTAGGCGTTTATACCTAGCAATAATATCAGTAGCGGTATAACCCTCTAAATGCCCAACGTGCAATCCCATTCCTGAAGGGTAAGGAAACATGTCAAGTATATAGGCTTTTTTGTTGCTATTATTTCCTGTGGCAAACGATTTATTTTCGGCTCAAAATTTTCTTCATTTTTGTTCTATTTCATTGTGCTTATACATAATATTTAAATTTTACATTAATATTAATTAAAAAAATAAGAAATAAAAAAAGTTGCGAAAATTTAATTGTTCCAAAAAATAATTAATTTAAAAATAAGCAAATGTATTGAAATTTCTAATTAATATGTTTAAAATTTTTCAATATTTAGAAAATATTACAAATTTTTATATCTATCCTGTAAAATTTACTTATTTAAAGACTAAAAACAAAATAAAGTTAAAAAAGTTTTAGTTTTATTAATAATAGAAATAAATTTAATCAAAGAGGTATAAACATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAAACATTTAATAATTTAAAAAATAAATTGCAAGATTTATTGAATTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTACAAACACAACAATTGATGATAATTCTCAAATACAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCCTAATAAAACTAAGACAAGATTTCAACAAAGCAAAAGAAGATCTAAAAAATCTTTTGGAATCAAAAGAAGTAGCGGATATTGAAAAAGAGATCGAATTAAACACTCTAAATACCACTGATATTGCCAAAGATTCTTTACAACAAGAAATTCAAACCAAAATACAAAAAATTCAAGATGCTATACAATCAATAAACAAAAAAATCAATGACAAAAAGCTATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAAGACACTTGAAAATTTAATTGCTACAGAAGATGCTAAAGCAGTTGGAATTGATGAAGCAAAAAAAGCTCTTGACAATAATAAAAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAACGATTTGAACGATATTACAGCCAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAACTTTCAAAAAATCTAAGCAAGATTTAGAAGAATATATAAATAAAAATTTAAATGAAAATAAATTTATTAAAGTTAAAGAAAAAGCCCAAAAAACCATTGAAAAATTTTCTAATATAAACAAAGATAATTCCTCAATCAAAGAAATTGAAGATGCTACTAGCTCGATAAAAGAGGCTAAAGAAGTTGCTTTGAAAGAAGAAGAAGGAATCAAAAAATTAAATGAAGTCTTGGAATATGTCAATAAAAAAAATGAACTAGAAGAATTAATAAAATCATCTAATACTCAATCAAATGAAGATGCTATTAAAAAAGCCCAAAAAACTTTAGACGATAATAAAGCAACAAATACCGATGAATTATCTTCCAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAAAAGCAATAATTGAACTAAAGGCAGCAATAGAAGCAACAAAAAAATCTAAGAATTAAAAATTTATATATGTTGAATTATTCATTCAACATAGTTAAACCCAAAATAATTTAAGTTGTTGTAAATTATTTTGGGTTTTAATATTAAAATGATTTTTGTACATTATATTTTATTAAAAAAATATATATGCCGACATTAAAAAGCAATTAATTGCATAAAAAATATAAAAAATAAGCCATTTTTTCAACCAATTATATTAAAAATCTGTTTTTTTTTTTTTTTATTAAGATGTTTTACTTTTTAATATATATAAAAATAAAATTATAAATAATAATGAAATGTTGAAATTACAAATATAAATTAATTTATTTTAAAAATTATGGAGTGTAGAAATCTATGGGTAAAAAAGCAAAATTATTTGTCTTTGGTGGGATTAGTTTTTTAGCAGGAGCTTCAATTATTGGTTCTGTTGCAGCATATGCTTCAATTAACAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTAGCAATTTAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACCTTAAGTGAAGTTGAAAAAATAGATAAAAATTCAGATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAACAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTAAATAAAGAAAGCCAAGGACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGACTATTAAAAGAAATAAATGAAAATCAAAAATATTTTTCAAACATAACGATTGATTCGGATATAGATAGCATAGAATTGGCAACAGCTAAACTGGTTGAAATAAAATTTTTAATTAATGATAAACAAGAAGCATATAAATTTGCCAAAGAATATAACGACTTATTAAAAGAAATCAAATGATTTATTTCAGATTGATATGAATTATTAAAAGATCCTGATATAAAAAAACACGAAGGTCCTTATGGTTACAACACGCCAGAAACTTTATTCGTATATTTTTTATTTAAATACAAAAATGATGACAAAAAAATATTTACAAAATTTAAAAATTATGCACAAGAAATGTATGACAAAACTGCACTTCTTGAGGAATTCGACATTGATTTTTTTGGATTCAAAATCAAAGATAAAAATTTCTATAAAAATTATGTTGAGTTGTTGACACCATATAAAAAGGCTATTGATTTGTGAAAAAATTATTATGATTCATATTCTATAGGGCTAAAAGAACTAAAAGAAATTATTTGAGACAAATTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGATAAATCAACCTGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTATTCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAACGACAATCAATAAAGGGGGCACGAAAATGAAAAAAAGAACAAAAATTATAACTTCATTAAGTCTATGCTTAACTACTGCTATTTCAACAATAGCAATCTATTCAATAATCGCCGTTAAAAAAAATATAGAACTAGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAGATTGCAATCGCTTATCTCAAATAATCAACACAAAATTCCAAATAGCTATTTAAATTCAATTCAATATTTCCATAATGTAACTAGAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAATGAAATTATTGACGTAAATGAAAAAATTCTTGCCTTTGATGTAGAAATAAAAAATAAACTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTACCATAACAGACTATATAAAATATGAAGAGCAGATGCTGGAGAACCAGATATGATAGTTGATTTTGGTTTTTCAGAAGAAGAAACTCATTTTCAAAAATATCTTGAAACAATTAAAAAATATTCTTGAATTTCTAAAAATTCATCATCATTTGAAATAAAAAGGGCTATCTTTGATTTAAAAAATATTGAAAATAATATTGATTATTTTTTAATTAAAAAAGAAGTTGAACATTTATTTGAAAAAATTGAAAGCACTATTTCAATAGTTGAAACTAGACTAGAAAAAAATAAAATCAATAAAATATTTGAGGATAATGAAGTAAAAGAAATTTTAGATTTAATTTCACAATTTAAATTAGATTTACAAAAACAAAAAGTTTTATATAACTCAGTAGAAAACAAAACTGATAACATCAAACACGAAACTTTTAAAAAAATATTTAGCCATTATAATAAGAACAATTTCTTTAAAATTTTTGATTGCATAGATCAAAGAGTTGAATTATATTACGACTTTATGTTTTTTTACTCGCCTTTATTCTTTTATGAACAAACAGAAGAAGTCAAAAAAGTTTTTCAATATCCTGATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAGATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCATTCAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACTAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTTGAAATAAAAAAAGCCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTCGCAATATTCCACAAGAGATAAAAAGAGTATTTGAGAATTTAGAACAGTAAAAAATAATTTGCAAGAATTAATAGACAAATATTAAAAATCCTTGAATTGTCCTGCCCTTGCAATAATCAAGAGATTATTCATACACAATACTAAAAACAAGATAACTAAAAATCATTATCTTGTTTTTATTTTTAATTTGGCAATATTGAAATTAAATTTAATTGACTTTTTGTCTAATTGAATGTAATTTATTTTGTAATTTAAGTAATATTTCTGTTAAATATTTTAAATTTGGCTGTGATTTCTTAACATAAAAATCTTCTAATTTTTCGTCGTGTTCTTCTATAAGTTTTCTAACTTCTTGTATTTCGTTAGAAATTTTTTGATTTTTAGAATAGACATCATTTGCAAGAATATTAAATTCCTCAACATTTGATGATATCAATTTCAATAAATTTCACCTATTTAAAATAATTTCCAGGTTTAATTTTCTAGAAAGTTCGTATGTCATTTCTATATTTTTATTGATTTTGGCAATATCTTTTGAAGAAAATAGCTCAGAATTTTCTTTTAATATTTTGGCTATTTCATTATTTAGAAATTTACTTAAAAAATTTCCATCTTCTGAATTTTTTTGTTCTTTTAAACTTTCATTAAGCGTTTTTAAAAGTTTAATGCTTTCTTCTTTATTAGTAATGTTTAAAAAATCCTTATTTATATTTTTATAAATTTCTTCTTGGACAGTTAGAATTTTTTTAAATTCTTCATGTATAGATTTTTTTATTTGTTGATCATTTCTATTATTTTCCAATTTATTAATTGTTGCTAAAAAATTTGTAATTTTATTTTTTGTTTCTTGACTAAATAAATTAAATAAATCGCTATTTTTTTGTTGCATTTCAAATTCATTCTTTGCATTATAAATAATTTGTGAATATAATTCAATATATTCGGCCAATAATTTATTTGATTCTTCAAATTGATAAATAACTTCTGCAAATGTTTTTGAATCAATATCCTTGAAATCATTAAATAATTCGTTCAACATTAGCCTATCCACAACATCTCATGTTTTGCTATTTTTAAAATTATTAAAGTTAATCAATTCTTGTATAAGTGGATTTAAATCATTCTTAACATTATTTAAATGTTTTTGTATATCTTTTGATGAATTTATTTTTTGATAAATATCTTCAATTCTTTTTGAAATTTCACTAGTTAATTTCGCTATATCTTCGTTTTTAAAGCTAAAATTAACATTATTAATCTTTTTAAGAAGTTCTATTTCCTTGCTCATATCTGTTTTGTTTCATTTTTTATAAATACTAAATAGATTTTCTAATTTTCTTTTTTGTTTCAAAAATTCAACATCGATTTTTGATTTTGGACTTAGCGACTCTTTAGTATATCTTTGGTCAATTAAATTCTTTTGAACGCTAATGTTTCAAAGAATTTCTTTAGATTTCAAAATAAATTCATCAGCCTCATTTTTATCTTTTTCAATAAAATCTTTAATTTCTAATTTTAAATTTTCATAAATAAAATCTTTAAGATTATTGGCTATATATGTTTGCAGTTCATTTTTAACTTCTTGTAGCCTACTAGGACTAAAACTTGCTACATTATTTTTATCTTCTTTTGGTTTTTGAGATTCATTTGGAACAGTTGGCTTTATATTAGGACTAACTATGTGGCTATTATCTTCTTGTTTTTCTTTAGATATATTTGGTTTAACATTAGGCGTATTTGCTACGTTGTTGTTTGAATCTTCTTTTCTATCTTTAGTTTCTTCTTTTTTATCTTGTTTATTAGAACATGAAGCACTAGAAATTACTGTAAATACAGTTGCAAACGATCCTAAGATAGTTGATAGAATAAATAGTTTGTTTTTTCGTTTCATATGTGTTTCTTTTGATTTGATATTAATAAATTATTTTTAAATAATTATTAATATAATATAGAAATATCAAAAACTTATATTTAATTTAATTGATTTTTAAAAAATTAATAAATTATTCTATTAAAAGTACAAAATTAGATTTGCTAACAAAAAATATATTTTTTTAACTTTTGTTTCTAATATATTATATAATTATTAAGCAATTTAAAGGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCATGTTGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCATTTCATTAAATTGTCCAAAAAAGCGCAATGCGCTTTTTTATTTTATTAAAAACCTTCCGATTTTTAAGTTTTACTATCGGAAGGTTTTTAATAAATTATTTATATTATTTGTTCATATTATATTTATTTTTATATTCAAGTAATTTGGCTTGTATTTCAGCTTCTTTGTTTTTTAGTTCTAAATTGCTATGTTTTTCTGAGTTGTCCAAAATTTCTTTAGCTTCTTGTATTAATTTATTTAAAGTATCGTAGCCTTCTTTATATTCAGCTTTTCCTTCAAGATTTTTTTTAATAAAAGAAGATGTTTCATTTTTTGTGTTACTCAATTTAGTTCAGAAATCTGCAAATTCACCAAGAATTTCATTCTTCTTATCTTCAATTGGTTTTTTTAATGCTCTAAAATCAGATTTAAATTTTTCTAATTTATCTTCCTGTATTCCTTGTTTTTCTAAATCAAGAGCATCTCATCTAATCTTACTAATTTCTTTTTCTGCTTCTTCTCAATATTTTTTGCTATCCAAACAGTTTGAAGAATCTGTTCGCATAGCTCCTGTAAATTCAATTACCTCTTTATTTACAAAGCAATATATATCGCAACTAAATTTATCTTTCTTTGCCTTTTTTAGGTCATTGGTTAATTGTCACTTAGCCCCAGATATTTGTTGAATGTCCGAAGCAGAAGTAACGTTTTCAAAGAACTTAAGTTGTTTGTTTAATTGGTCTATTATATTATCATCATAATCATAATATTGACTACTATATTTTGTATAATATTCATCCTCTTTTACTTTTTCAATAAATTGTTTAATTTGTTCCCTAATTTTTTCAAGCCCATCATATTCAATGTTTTCCTTTGGCTTTTTATCATTAATTTTATTGGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTGCCAGTAAGATTTGTGTTGTTAAAGATGTTGGTTTCTTCTTGTTTATCAACATCATTGGCTTCATTTGATTTAATTAAATTATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTAGCTTGTTCTTTGGCTGCGTCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAGCAATTTGATCGACTGTTGATGAAGCATCTACGTTGTTATTTTGCAATGATTGATTTGCTTTAGAAGTGTCAACTTTTTGGCCATCTTGTGAATCAATTAAATCCTTTAATTGCTTTTTAGCGTCGTTAAAGTCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTCAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCTTTGTTAGTAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTTGCTTCATTTGATTTAATTAAATTATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGTGGCTTTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCATTAACAATTTGATCGGTCGTCGATGAAGCATCTACATTGTTATTTTGCAATGATTGATTTGCATTAGATTTGTCGACTTTTTGTGCGTCTTGTGAATCAATTAAATCTTGTAATTTCTTTTTAGCATCATTAAAGTCTTTAAGAAGATTATCTTTTTTATCATTAATTTTCTTAGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTGGCTTTGTCTTTGGCTGCATCAATCAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATTAACAATTTGATCGGTCGTCGATGAAGCATCTACATTGTTATTTTGCAATGATTGATTTGCATTAGATTTGTCGACTTTTTGTGCGTCTTGTGAATTAATTAAGTCTTCTAATTGTTTTTTAGCGTCAGTAAAGTCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTGGTCAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATGTCGGTTTCTTGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTGTCTAGTTGTTGTTTCTTTGAATTAAAGTCTTGTTTAGCTTTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTTGATGAAGCATCTACATTATTATTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGACCGTCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCGTCGTTAAAGTCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATATCAGTTTCTGCTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTTTCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTAGCTTTTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATTAATAATTTGATCAGTCGTTGATGAAGCATTTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGAAGTATCGACTTTTTGGCCATCTTGTGAATTAATTAAGTCTTCTAATTGTTTTTTAGCGTCAGTAAAGTCTTTAAGAAGATTTTCTTTTTTATCATTAATTTTCTTGGTCAATGATTTAATAGCATCTTCAATGGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGCGGCTTTTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTACATTAATAATTTGATCGGTCGTTGATGAAGCATCTGCATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGACCGTCTTGTGAATCAATTAAATCCTTTAATTGCTTTTTAGCGTCATTAAAATCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTGGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCTTTGTTAGTAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTTATTTGATTTAATCAAATTATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGTGGCTTGTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATCAACAATTTGATCGGTCGTTGATGAAGCATCTACATTGTTATTTTGCAATGATTGATTTGCTTTAGAAGTGTCGACTTTTTGGCCATCTTGTGAATCAATTAAATCCTTTAATTGCTTTTTAGCGTCGTTAAAGTCTTTAAGAAGACTGTCTTTTTTATCATTAATTTTCTTAGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGCGGCTTTTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATCAACAATTTGATCGGTCGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCGACTTTTTGGCCATCTTGTGAATCAATTAAATCTTGTAATTTCTTTTTAGCATCATTAAAATCATTAAGAAGACTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGCTTGTTTGTCAACATCTTTAGCTTCATTTGATTTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTGGCTTTGTCTTTGGCTGCATCAATCAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATTAACAATTTGATCGGTCGTCGATGAAGCATCTACATTGTTATTTTGCAATGATTGATTTGCATTAGATTTGTCGACTTTTTGTGCGTCTTGTGAATTAATTAAGTCTTCTAATTGTTTTTTAGCGTCAGTAAAGTCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTGGTCAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATGTCGGTTTCTTGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTGTCTAGTTGTTGTTTCTTTGAATTAAAGTCTTGTTTAGCTTTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTGATTTCATTTGTTGCATTAACAATTTGATCGGTCGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGACCGTCTTGTGAATTAATTAAGTCTTCTAATTGTTTTTTAGCGTCGTTAAAGTCTTTAAGAAGATTGTCTTTTGGTTCTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCATTACCAGAAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGCTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTTTCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTAGCTTTTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCATTAATAATTTGATCGGTCGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGATGTATCAACTTTTGGCCATCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCGTCAGTAAAGTCTTTAAGAAGATTGTCTTTTTATCATTAATTTTCTTGGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTTATTTGATTTAATTAAATTATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGTGGCTTGTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATCAATAATTTGATCGGTCGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCAACTTTTTGGCCATCTTGTGAATCAATTAAATCCTTTAATTGCTTTTTAGCGTCGTTAAAGTCTTTAAGAAGACTGTCTTTTTTATCATTAATTTTCTTAGTCAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTGTTAGAAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATCATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTGCGGCTTTTTCTTTGGCTGCATCAATTAATTTTTGTAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATCAACAATTTGATCGGTCGTTGATGAAGCATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCGACTTTTTGGCCATCTTGTGAATCAATTAAATCTTGTAATTTCTTTTTAGCATCATTAAAATCATTAAGAAGACTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTGTTGTTAAAGATGTCAGTTTCTGCTTGTTTGTCAACATCTTTAGCTTCATTTGATTTAATCAAATTGTCTAGGTTTGATTTTAATCTATTGAATTTTTCCTTTAATTTGTTAATATTATCTTCTTGTTCATATTGTCTTCTTTTAAATTTGCTTTTTAGCGATGTTGCAACTAAGGTGGCCGTTGTTGCTAGAGCAATAATCAACGAAGTAGAAAGTAAAATTCTTAATTTCGATTTTTTCATATATCCCCTTTTTTTATTTTTCCCTTATTTTTTATATAAAAACTTATAAACAATTATTAAATTCTTATATTGTGATAATTTTCAAACAAGACTTCGAAAAAAAAAAAAAAAACGCTTTTCGTTCTATTACAATTTTTTTATGCACCTTTTAGTTTATTTTAATTGCTTTCTGTTAAAACATGCTTAAAATAAACTAAAAAATCAAACTCATATTTTTAGTTGAGTTTGATTTTTATTTGCTTTGGATACGTTATTAATCTTGCTGAATATCCTTATTTGAACCTATATAAGCAAAAATTTCAGGATTTTGTTTTAAGTATCTTCCTTCCAATATTCTTATAACGCTTTCAACTGTTTCGTTTAATGGCACAAATTGACCAGGAGATTTAGTAAAGTGTTCAGTCATAAAGAAATTTTGAGTAAAAAACATTTCAAGTTGAAGAGCTTTTTTAACAGTGATTTTGCTTTCTTCATCAAGTTCATCAAATCCCAATATCAGAATAACATCTTCAAGATCTTTATATGCCTTTAATATTCTTTTTGCTTCAATTATTGCATCATAGTGACGTTTGCCAATAATGGTTGGATTAATTGCGTTTGTAGATGAAGCAAGTGGGTCAAAGGCAGGAAAAATGTTTTTAGCTGCTTGAGATCTAGATAAGACCAAATTACCATCTAAGTGATTAAATACAGCTACTGCAGAAGGGTCGCTTAAATCATCCATTGGTAGAAATATTGTTTGGAATGAAGTTATTGAGCCCTTCTTGTTTCTAAACAATCTGCTTTCAACTTCGGTTACATCACTTTCTAAAGTTGATTGGTAACCTCCGATTGAAGGCTTTTTGCCTAATGCTGAACTTACTTCATTTTCTGCTTGAATAAAACGATAAATATTGTCAATAAATAACAAAACGTTTTCGTTGCATGTGTCTCTTAAATATTCTGCAGCAGTAATTCCGATTGGAACAATTGACGTTCTAGCTCCTGGTGCTTCATTCATTTTCGAAATAAACATTATTGAGTCCTTCATTAAATTAGATGAAGAAAGTTCATTGTATAATTCTATTCCTTCTCTTGAACGTTCACCCGAACCAATAAAGATATTAGAATTTGCTTCATGTTGTTTATTAAGGTTAAAAATAATTTCCTTCATTAGAACAGTTTTTCCAACTCCGGCTCCTCCAAATAAACCGATTTTTGCGCCCTTAACAATTGGCATAAAGAAGTCAATTGCTTTTATTCCGGTTTCTATAACTTCTTTGCTATTTTCTAGATATCTTTCATTGTTTATAGTTGAATTCATTTCTACATATTCAATTTTATTCTTTGTTTCTAGTAAAGGTTGACCTCAAAAGTTGTAAATATTGTTTTTTGAATTTTGCCCTATTGGAACTAAAAAACTCTTATGAGTGTTAACAACTTCTTCGTTAATTGAAATATCTTTACTTGCATATACAACAATTGCTCTAACATGTTTTTCGTCAATTAATCTTTTTACTAGTAAAAAGGTTTTTCCTTCATGCAATGTCAACAAGTGATTGATAGCAGGTAAATCTTTATTAAATTCTACTTCAACTATATCTGATCAAATTTTTATAATTTTTCCATTCATTATTTATCACCTAAAACCTTCGCTGCCTTATCTAATTGTTCTTCAGGTATTGGCACAAAATTATGTTCCAATTCAATATCTCAATTTAAATTTAAATAATCTGAATATGATTTCAAGACAAATGCAAAATAATTCTTCATAATATTATCATCGTAGTCTAAATTATCTAAAATTGTTTTAAATGCTTTTATGCAATCATTATAATTATTTATTAAATAATCTAGTAGCATGAATGCTTTTTGTTCATCTTTTATTCCTTGCAAAATACCTCAAGAAATTAATTTTGTTGTTAGCACTACAAAACGATATGAGTAAAGTGAAAAACCCTTTTGCAAGAACATTTTGTCAATTAATTTTCCTTTGTGAAGCAAAATAGAAGTTTCTTTATTAAGCTCGTAATCTAACATTGCTAATTTTAAATGTCTCTTGTAACTTCTAAAAATTTTATTAATTTCACCAGCTATTTTTGTCATCATTCTTGATTGAACACTTGAACCTGTTCTTGATACAGATAAATCAATGTTTATAGCAGGTAATTTCCCTGAAGCAAATAAGTCAGAAGAAGTTACGATTTGACCATCAGTTATTGAAATTATATTAGATGAAATTAAACTAGTTATATCGCCGTCAACTGTTTGTAAAATTGGAAGTGCAGTAATTGTTTTTCTATTGACAAAAGATCCAGCTCTTTCAAGCAATGATGAATGGGCAAAAAAGATGTCGCTAGGCATTGCTTCTTTTCCAACTGACTTGTTAGTTAATAGGGCAATTTCTCTAATAATATTAGCGTGTTTAGTTAAATCATCAAATACTATTAAAACATCATTGTCATATGAAAGATTTTCTGCATGGGCCATTCCAATGTAAGGCGCTAGATATTGTTCATAAACGTTTTGAGGTGCATCAATAATCATAGTGTTTTTCAATGCATCGTATTTTTTTAATGTTTCATAAATATTAGTTAATGATTCACGCTTTTGGCCAATTGCAACATAGATACATTTTGTACCATTTTTTGCTTGATTTATAATTGCATTCAATGCCATATGAGTTTTTCCAGTTTGCCTATCGCCAATAATTAGTTCACGTTGACCTTTTCCAATTGGAATTAATAAATCTATTGCAATAATTCCAGTATAAAGTTGTTCATTTAATGTTTTAACTGTCATCAAATTATGAGCTAGGCCAAATATTGGATGATGATTGCTCAACATTGAATTATTTGATCTATTTTTTTCATTAGTTGGTCAAATGACGCCACCATTAATATCAATAATTTTGCCCAAATAATCAAGACTAGTATTTATATTTTGGGATTCATTTAATTCAATTAATTCACTATTTATATCTAAAGATACGTGTTCACTATTAATCAACATAAATGCCTTATTAGTTGTAGCAGAAATAACTATGAATTTAACATTTGGGTTACTTGCTAAATAAAATTGTTGATTTTGACGGTAGTTGTATTCACCCTCAATTGTTGCTATGCAATTGAATATTGATTTAATACGAGGATTTTTAGTTGTGTTGTTCATGTGTTCTCCTTCTAACTATATAAATAATAGCCCTCCAATTATTAATATTAGTAATGCGATCGCTAGTATTACACTATTGATAATAATTACCTTTTTCTTTAATTTTAAGTTTTTTAACAATGCAAAAATTATTATGTTTGTAATTATTATGATTAAAGAAATAACTATAAAAGAAGTCCCTAGTTTTTTAATAATCTTCTTTTGTTCAAGATAATTTTTATTTAAAACATTGACTGCTTCTAAATAAGCTTTATTGAAATTTTCTTTTTCTTCTTTATTAGCTTTTATTGAAATATTACTTGCTAAATTTGATAATAAGTCAAAATTATGTTTTATACCAGACAATAGTTCAAGGTAATCAGAATATCATTTTTCTTGGTTGAAATCTCTTTGGCTAATTAAAGCGGTAAATTTGTAGTTTAATTGTTTATTTAATAAGAATAATTCTCTTAAAACATTCTCATTGCCTTTGATTTCTGCTAAATTTGTTTTAATGAATTTTTCATTGAATTTAAGCGCTTCACTAAATTGCAATTGTACAAGATTATATGCCTTTGCTATTGTAGTTCAAAAGTATATATTATTTACTACTAGACTATTTAAATAAGAAAAAAATGTATAACGAATTGTTTTACTAAACTCTTCAAGTTGTTTTTTTGTATTTGTTTTATCTCCTTGCAATTTAACTCAATATTTTTGCAATATTTTATTGCTAATTGCACTATATGAATCTTTATCAGCCTTAAATGACATTTGTAACGCCGAGTCAATCATTGCAAGCAATGCTTTTCTTAAATATCCATTTCTTAGCTTATTGTAATCTAACTTTTCATCTAAGCCCAAACCTTTATATAATGGCTTAAATATTTCTGAAATTGCTTTTATTTCAGTTTCTTTTAACAGATTAAAATATTTGTCTTGTGACGGAGTATATTTTTCAATTAAATATGTTCTTTCTAACTTAGGATTTATATTATCTGAAATATGTACTTTTAAATTAATAATATCATTGCCACTAATATCAAATGAAAAAACAGAATATTTATAACGAGTATTTATTGGGTTATTGAAATAAAATAATTCTTGACGTTTGCTTGCATCTAATGAATCAAATTGGCTTTTAATTTGACTTAATGAAGAAGGCAAATTGTTGCTTGATATAAACGGAACCAAAAGGGGCAATGATTGAATTAAATTTTTTTCTTGTTCTTTTGTTAAGTGGCTTGTTTTATCACCATCAACCAATGGCTTTAAATCAGGCAAGGAATCAGGATTTTTTTCTTCTTTTTGTTCAGCTTCGTTTGTTTCTTGATTTTGTTTTAAATCAAACTTTGTAATTCTTACTTTAAACTTTGAAATAAAATATTCATCTCAACTTTTAAAGCCTTTTGGCAAGGTGGTAGAAAATACTCCATTTTTATCCCTAATTAGTTCAACATCTTTGTCAATATTTAAAACATCTTTTTCATTAAAAAATAACTTATTGACTTCTTTTAAGAAATCAGATTTATATTTCTTTAAAAATTGAGAATACTTATCTTTTTTAACAGTATTTAATTGATCTTTAGCAACTTCTTTAACACTTCCTTTTGGCAAAATTGCTTTTTTATAATCTAATGGATCGGATTTACCAGTTTTTATTGATTTAAATAAATCGCCTTCGTAATCAATATCTTGCAAATCATGGTTTTTTTCTTTTCCTAATATTAGGGGAGTATTTAAATAAATTCCATACTTATTAGGATTTTTTTGAATCTCTTCTTTATTTTTTGTTAGAAATGTTTCTAAATTTTTTAAATAAATTAATTGTTGAATTTTTAATTTAAATTCTAATTCTTTATTTTCTAGCAACCTTTGTTGTTGAACTTGCAAATAATTGATTATTGCTTTTAAACCATCTTCTATTGTTGAAGCAATTGTTTTTTTAGCTTCACTTTCAAATTCACCAAAATTTGGATCTAACTCAGGTTTTTTATTATTTGGATCATTGTTTGGCGCTGCCGAAACTACTATTGGAGATAATAAAGGAACTGTTGCAAATGATGATAATAATAAGATTTTCTTTTTATTCATTGTTAATCCTATCTTTTTCAATTAAATTTAATTCTTGTCTCAAAGATAACAAGTAATATTTTTCTTCAAGAACTTCTAATTTTAAAAATTCTTCTAACTTTAAGCCTAATTGTTGTTTAGCCTTTCAAAATTTAATTTCGTCTGAAAGTTCAGCTATTGCTTTAACTAAATTGACTTTATTGAGCGATTTTATATAAAAATGAGGTTGGGTTAATGTTGAAATTGTAGCTATATTATTATTTACAACAATATTTGAGTTTTTTAAAAAAACAAAGAAGGTTTCGTTTTGTTTTGAAATATCAATAATTTTTAGTAAAAGGTTTTCATAAGAAGCAATTGATGTATTTGCTATTTGTTCTCATGTATTTTCTTCATTGACATTAATATAAACTAAGCCTTTTTCAATATTTAGCTTCTTATTTTCTAAAAAAAGTAATTCAATTTTATATAAATTATTCATAAATATCACCTTCATCAGGGTCTTTTTTAGTCAATAAATTTAGTTCCTCAATTTGTAATTCACTTTTTGCTCTTGATATTTGCTTTTTAAGTTTAAGAATACTTTCGTCTAAATCTTTTAAAATTGTATTAGTTGAAACTAAACCTATTTTTGCTTTATAAAATGCAGATTCAGATATTAACATATTTATAGTGTTTAATAAATAACAATTAATTTGAGAATTAATAAAATCATTAATATTAGGATATATTTTTGCTTTTTTAAAATCTATGTCATATAAATTATTATTGCTTTCATTTAAAGGAAATTTTTCAAACGAGAATTTAGACATTGGCAAAATTGTAAAATATTGATTAAAATTTTTATTGGAGTTAACTACGAAATTGACTTCTTCGTAATTTTCATATTCATACAAGATCATTACTATTTTGGTTAATTTTTCTGCCAAATTTTCAATATTTGAATTTCCATAAGATTGCAATACTTGTAGGTTATTATCTTGACCAAATTTAATTGCATTTTTGCCAATTAAAATGAACTCATTATTAGATTTTTTTATATTACTTAGAATAGTTTTTTCATATCTCGAATACGAGTCGGTGCTATATTTTTGTTCTTCTGTTAAATAAATTCACAATATGTTTCTATTTATTTTTCTAAATAAATTAAACCTATGATGACTTTCTCTCCTTAACATATCATTCTTAATATTGTAGATTTCTTTTAAATGCAAAATTATTTGCCTTGATACCAATGCATTTTTATTAAAAAAGCTTAATCTTTGGTTAAGTTTCATAATATTTATTAAATAAATATTTTTTTCATTATTTACTTTTAATGAAATATTGTTTAAACTTTTGCGTTTATTCTCTAAATCTTTTAGTTGCATGTTATTGTTCCTTTGTAACAACTGCTACTACTTTTTCACTAAATAATAAAACTAAATCATTTTTTAATTCTGAATGAAATAAAATTACATGTGGAAATATCTTAATTGATTTTTTAAGGGCCAAAAGTTTTTTTATTTCAGCATTATATGAATTCAAAAATTCTTGGTATTTTATATCGCTAGAAGTGGTTAAATCAATTGCGTTATTGGCAGAATAATTTTTTGTTGTAATTATAGGTACTAATACCTCTTGTGAGAATTTCAAATTCAAATTAAAAGTAATGTTAAAATCCTTTAGTAAAATGTCATATTTATTTTTAAAAGCCAAATCAATTTTGCTTATAAAATCTAAATAAATAGGAGAATTAAATCCTAGGTTGTTATTAAACAATACTGTTGATATCATTTCATCAAAAGTAATTAAATCTTGTGAAGACTCGTGTTCTCTATATTCTTTAAATACATCAAAATAACTCAATAATGGATTATTGTCTTTCTTTTTCATATTCATTCTCCTTATTTAATTTTCTTATATAATAAATTGCCAAAATAAATTTAAGCAATAAAGATAATGCCATAATAAATGCAATGCTAAAGTTTAAAAGTAAAATAATTTGAATAATATTCATTCTACTTTTAATTATTGAAAATAAATAATTTTCATTGCCATCAGCAGTTAAAATATAATTAAATCCAATAATTGCAAAGATTAAAATTGAAGAGATTATTGCTAGTTTAATTATTATTAATAGTAGCAATGAAATATTCTTGTTTTGTTTAACAAACACAAATAATATTACTATTTGTGTTGTTGCCGTTACAGCAACGTTGATTAAAGAAACAATGCTATTGGTGTTAAATAATGTAGTCAATAGCAAACTTAACGCAAGCACTATTTGAACAATTGCTAAATTCAATCACGATCTAAATGAACTTTTCATTTCAAAACGCTTTTGAAATGAAACAACTATATATGCAATAAATACTACAATTGCAAAAACGCTTTGAAAAATGTAAATTCATGTTGTTTTTGAAGAGCCTATTGAAATGTTTTCATTATTATGCTTATATGCAAAAATTCTTAGAATTCAAATAAATACAGCCCCAAATAATACTAATAAAAATAGTAATTGATTTTTTAAATAGAACTTATCATATTTTTTATTTATCAATAAAGCAAAGAATTTAATATTAGATAATACTATAAAAGTAATTAAAACTAAAAATGTCAATACAATAATCACAAAATTGCTAGCAGATTTAATTGTTAAAATATCTCTAATTGTTTCATAAAATTTATTGCCTACAAACAATATGTCTTTATTAATTCCTAAATTAATTCAAACAAATGATATTGCAACAAATAATATTGTTAAGATTCCTTGAAATGTTAAAGAAATTATTAAAATATTTTTATTCTTATAAAGCAATGGATTTGTTTTTCTTTGAATTAAGAAGTTAAATATTTCATAGCTAAAATTAATAATATATAAAGCAATCAAAATAAATGATATTGTAAATAATTTGCTTGGAAGAATTTTAAAATAGCTAAAAAATAATGTATAAGAAGTTAATGAAAGCAATAAATACAAGAAGTAAAATGGAATATATTTTTTAATAGTAACCTTATGTCAATATAAATTAGCAAAGCCTTTTACTAAACTATAAAATAAAACAAAGGATAAAATTAAGAATCTATAAATTATTAAAAAGTTTAATTGTTGATTTCTTGGACTTGAAAAGTCAAAAAATGAATTTAGTGAATTAGAAAGCAACATTTTATTAGCAAAAAGTGTCTTTTCGCTAAAAATTAATAAAGTTGTAATAAATACAAGACTAATTACAATCAAAAGGTATTTCAACACTTTATTTAAAATGTTGTTTTTCTGCTGATTTTTTATTGAATATTCATCATCATAAATCAACTTTTGATTTATATTATTCGAATCGATTTGTTTTTGTTTCATCTTATTATTTTCCTATTAAATAAATTCTCCATCAGAATCAGAAGAAGAAGAGGTTTTTACACTATATCCCAAACCTTCTAATTGAGATTTTAAAGCTGAGGCATTATTATCAACAATTATTTCTTTTTTCAATTGTTCTGCAAACATATATAAACGCGAAAGGTTGTCAATTCCTTCTGAATTTAGTAATGAAGTTCCTGTGATTCTTACTTTTGTTGTTAAATTTCCATTACTAAATAAAATCTTGCTTTTTTGACCAGGTTCGCCTGAAACAATATGTCTATTAAAGCCTGCTTCATTTAATTCTTCACCTGATATATTGAATGCTTCACTGTTATTATATAAAGTAAGTCTTCTTATCATTCTTGATTTTGAATTACTTGGTTTTAATTTATCATGAAATTCTAATCCCCTTAAAGAACGTATCTTTGGTATTCTAGTAAAATCTAGGCCTTGTGGATAACTATTACCATCTTCTTTATGATCTGGTTCTAAGCCCGGACCAAATCCACCTTCAAATATTGGTTCATTATTTCTAACTCAATAAACCATTCTCATTCCATCGTTAATTCTCTTTAATTTTTCTTGTAAAGTAGAAGCATTTTCATTGTAATCAACAGGATCAAAGGCCAATGTATCAAATGTTATTCTTGTGGCGATATCAGCGCCTTGTTTATAGTCTCAAGAAACATTGTAATCGTTTGAATTTATTCATTCAACTTTTCTTAATGCATTAGGGTTAATTGATCATTTATCCAATAGCGAATTTCCTAAAGTGTAAATTCCTAGTTCCTTTATGTGTTTATTTTCTAACGCAATTAAAGAAGAAGTGTTTGAAGAACCTGCTGAGAAAAATAGTTCTAGTAGTTGAATTTCATCAGGAATTGCCTTTAAAATATCCATAAATTTTTGGCTAGAGTCTGATTTTCCCATATTTCTAATTCTGTAACCAGTAATATTGACCTTTTTAACCTTTAAATCTTCAATTAATTTTTTAGTTTTTGCATAACCATCTGGGTTGTCAGCATCAATATCAACAATATAACCTTCATTGATCTTTCCGTCTTCTTTTTTTTCTCTAGTATATTTATTAACAGTTATACCATCGCCATCTTTAATACCATATTGTTGAAATTCCTTAGTTACATTAGTTTTCTTTCAACCAGGATAGTCGCCATTAGCAAGACCATATGGGCTTCTATTTTCAATACTATCATATCCAAAAACTCTTCTATTTTTATTGTCGTTTATTATTCTATTTGCAACACCATTATAGCCTTTGGCTGGAGAATATGCATATGAATCTAGCTCACCTTTTTCGTTTACATAGGCGTTATCTTGATCTAATGCAAGGCCTTCAGCTAATTGCTTTTCAGCATTAGAAGTAATCTTTGTAAATTTAGAGAAATCTAAATGAGAATACAATCAAATATATTTGAAGTGTTTCCTGCCTTCAAATTTAGGCTTCATTTCTGGATATTTTGCCTTGCCTTCTGCTGTTAAGAAATTAACAACATTTTCTGAATCAAATAATCTTTGGAACTTTAATAATTGGTTTGCGGTGTAACTTAATGAACCACGATCAAATTCTCTTTTAAGATAATCTTCGGCTTCATCTATTCAATTTGCCCCTCAACTTTTTTCATAATCTTCAAGATCTTTTTTGAAGTATTTTTCATCTCTTTTTAAACGTTGAGTAGCCGCTTGGGCTGCTGCTTGCTTCAATTCTTCAGTAACCTTAACTTTTTTAATTTTTGGAGCACTATCATTGATGTATGGGTTTAAATTAGTTAGATGATTTAAGATATCATATTGATTATATTCACGTTTCTTTTGATCTTCAACTTCCGCTTCAACTTTGACACCGTTTAGAATAATTTCAGTTGTTCTAGTTGAAGGTTTTGATGAAGGGGGAGTAGGCTTAGGTTCGGGTTTTGGTTTTGGCTCTGGTTTTGGAGCTGGAGGGGTTGGTTGAGGTTGAGGTTTTGGCTCTGGCTTAGGTTCGGGTTTTGGTTTTGGCTCTGGTTTAGGAGCCGGCGGAACTGGGATTGGTTCAAGCTCTGGTTTAGGGGTTGGAATTGGTTGTGGCTTTTCTAATTCTTTTAAGTTGTTATCCTTTATTGAAGGCAACGCATTTTTATAATCTAGGTTGCTTTTATTTATAAAATCATTTCCTGTTGCTGAGCTGGTGGTATTAAAACGAGAATAATTTTGCGAACTTGTACTATAAAATATAGCCCCAGCAATTATTGAAGCTCCTATAACAACACCTGCCGTCATTAAAATTAATTTGTTTTTTCTTTTCTTTAATAAGTTTTTCATTTCACCACTTTTTTGAGTTTTATTATTGATTATTTTTTATTTTCTATAAATTTAAATTCATTAGGAACTTTATCATCACCTAAGCCATTAGGGAATAATGCTGTTTTAAAATCGCTATTATTTTTATATAGTTCTTTTAATGTTTGTCTAAATGAATTTTTTTGGTCTTTACCGCCGCCATAAATTAAATCATATTGAGGCAAATTGTAATTTCCATACAAGCCTTTGTAGTCAAATCCTTCCGATCTAAAAGCAGCTGCTAAACCGGTTTTTGCACCTAAATTTGATAAATAAAATATTCCAACTAGTTCGTTATTTTGATTTCTTAAGCTTGTACCAGAAGCACCACCAACTGGAGCAAAATGTTTAGGCATATAATTTAGCCCCATACGCATATATGTTTTGCCATCGCTACTTTTATAAAATGAAAATACTGATTTTTGATATTTTTGGCCATTTATTTCTTCTTGAACTTGCTTCATTCCACCTGGTTGTGCTAAAAACACATCAGAAATACCGGGTTTATCAATAAATGTTCTATATCCTAAACTATAAGATAAGTAATTTCCTCTTTCTGTTTTTGATGGAGGAAATGAAGGGGTTTCATTTTCGCCAACTGCTAATTGATCATAAAATCTATAATCCGAATTTGTTCACAAACTGTAATTAAATTCATCTCTTCTTTTAATTTGGTCATCATCTACATATCTTCTTAAAAAGAAATCTTGGGTTGAATTTGGATAACCTAATGCAAAAAGTTCATCATAATTCTTTCATCATTCCTTATTTGCTTTATTTGCCTTTAAAGGAACATCAATTTTTTGGTAATTTGATAAATATGATTTCGACAAGAATTTAATTTGTTTTGCTTTATTTTGAGCATAATTACTTGTCAAGGCTTTTGCTAATTCATGGGCATCTAAATTGCTGAATTTTTGAGTAATATCGCTATTTTCGCTTAGATCATTTTTAGAAACGCTTTGTAATGTTAATTTTTCAAAATCAACTTCAAAAACTGCAAAATCAGCATATTCTTCAACATCTTTATACTTTTCTTTTTGTGATTTAATTAAATAATCAGAAGGTTTTAAATTTAAGAAATCTCTTGCAGTATAAACTGTCTTTATTCCATTAGAAACATTAGTAGAATCTTTACCAGTAAATGAAAATCTATATGTATTTTCATCAAGATTTGCTAAACGCAATGTTGTTTTAATTTTTGCACTATCCATCAATTTTAGAAGTGTAAATGAAGTTGTAAGTTGTTTCAATGCATCTGCAACGTGTAAATTTGTTGCAAAATATCATTTTGTTGGATATTTTCCATCACTTCTTTGTTGATAATCCATGATTCAAGTTGTTCCAGCTGTATTAGTAAATTTTCTTTCTTCGCCTTCAATAGGAGTTAAGAATCTTATTCCAAATGTTTGTGAAGCAATGTCACGGTAATGTTCATTTGGCAATGTTCTTGCTAAACCTGTAATTTTATATGGATCTCTACCAAATGCATCTGTAGGTGAAGGCCCTTGACCTCCTGGAGTTTCATTAATAGCAAGGCCTTCTACATTGCCTTTTGAATCGTAGCTTGGCAATGTAAAACCTTTTCTTGCAGCTGATTCATACGAATCTTGATTAATTTCTTTTGCCTTAGCATCAAATTTTTGAATGTTAGATTGTGAAGCATCCAAATTTGGTCTAATTTCTTTTATTGATTTTTGTTCTTGTTGACGAGTTAAAATGTCTAAATATTTTTTATTATCTAGCTCATATCTTTTTGTTTGATTTCCATCAAGATATTGTTCCAACTCACTTTTATTCAATCTTAAAGCATCAGAACCTGTATTAGGAATTGTTCCATTTTCATCAAAACCATAAGGATTTGTTTTAAATTTTCCTACAGTTATTGTTTCAATAAATGTTTTGTTTGTCTTCTTGTCTTTAATAGTAATATTAAAATTCAATTGACCGGTTTTGTTTGCAACTGATCAATCATCAGAATTGGTTGAAGTTTGTACGTATAAAACATTGACTATTTTTTTAATTTCAATTTCATCATTAAATTTTTTTTGTTTAAAATCAACACCTTGATTGAAATTTCTAACAATTTGAGATGGAAGCAAATCTTGTGAATTTTGATTTGAAAAAGCCTTGAATTTCAAATCAAATAATTGACTTGGATCATTTAATGCTAATTTTGAAACTTCAGAAATTATTTTTTTATATTCGGTAGTTTTTTGTTCTTCAACTGATAGTTCATCTGTAGAATTATTATTTTTGTTGTCGTTGCCTTTTGAATCTTGATTATTTTGGGGTTTTTCATCAGGATTCTTTTTTTCGTCATTTTGATTATTATTAGTCTTTTTATTTGTATTTTTACATGAAATAGCTACAGCAGACATACTAGAAAGTGATGTCGCTGCTATTAATAATGATATTATTAATTTAGATTTTTTCAACCTTATACCTTCCTTGTTTTTTAATTTTAAACTTGAAAAATAGGAACTAAATTTCCTTTGATTTTTTATTGATTTCCCTATCTTAAAAAAACAAATATTTCCATTTTTCGCAATAATTTTACTATATTAAATTTAAAAAAAAAAAAAATACTTCAATGTTTAAAAAAAATACTTTAAACCTTTTAAAATCAATATTAAATTCTAGGGATCTAATTGAAATTAAGATAATAAATGCATATCAAGATATGCTTTAAAAAATGAAATTTTTCATTTTAATGTATAATTTAAAAATTAATATTTGAGGAGGCAACATGTTGGATGTTTGCAATTTAGCTAAAGTTTTTCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAATGGGGTTGGAAAATCCACTTTTTTAAAAATATTAAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGATGATTATATCGCAACTGATGTTGTAATAATGGGAAATAAAGAACTTTATCAAATTAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAAAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGAAATGCTGAAAACGACGCCCAAACATTATTATCAAATTTAGGAATTGAACCTGAAAAATGAACTATGAAAATGTCGGATTTAAAAGCCAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCTGATATTTTAGTAATGGATGAACCAACAAACAGATTGGACCTAAAAACAATTAAGTGATTAGAAAACTTTTTAATGGATTATCCAAATATTGTTATTGTTGTTTCTCACGATAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGATATAGATTACTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGAAAACAATCAAGCCAATTGTTAATAGAAATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCCACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCCTCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCCGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGATACTTTATTTGAAAATGTAAGTTTTACAATCCACCCTGGAGAAAAAATGGTTATTATTGGCAATGATGACATTGCCAAAACTAGATTACTTGAAATACTTATGGGTAAGCGTAAACCAACATCAGGAACAGTTGAATGAGGAATAACTATTAAACCAAATTACTTCCCAAATAATAACTTAGAATATTTCAAAGAGCCAATGTCAATAATGGATTGAATTAGCAAATGGCCTCTAAATAATACAACTCAAGAAACAAAAGATAATAGTGATTCAAGAATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAAAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATTTTCGACCAACCTCTTGACCACCTTGATAGTGAATCTATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGAGCAATGATAAAAGAATGTGCCTCAGTTATTTTAGAAATAAAGCCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAAATAGCTAATTAAAAAACTCGTAATTAAAACTACGAGTTTTTATTTACTTTTTAAAGTTTAGCAACTTATCTCTATAATATTCGTATTGTTTTTTACGTTGATCAATTTCAAGAGGAAGGCCGGATTGAATATCTTTGGTATATGTTTCAAGTTTGTCAAGAATTTCAACTATTTTATTTTGAATTGAAATGTGGGGAATGGGGAATAATAAATTCCCAAGTTCTTCTTTTTTAATTAAGGGTAAATTTACGTTTTCATATCTTTGCTTTAAGGCATTTGAAAAGTTAAAAAAATAATAAAACATAAACATCGGGTTTATTTTAGCCACGAATTGATCTTTGATAGTAAAAATTGAAAATTGTTGATTGCAAAAAAATGGTTCTAATACAATAGCATGTTCGCCAATTGTTGCAGTCGTAGATAATATAATTGAATTTGCTTTAAAAATTTTTTTTATTTTTAAAACACTATTATCTACCTTAATCGTTGAGCTTTTTAATACTCGACCTTTTTGTCTAATATCTTCAAGTTTAAATCAATTTATATTTTCATTTTTTCAGTATTCAGAACGATTTTTTAGAGGAGTAAAACCACCAAATATGTCGCAAATATCCTGAATTTTTCTATATTCAACTATATTGACAATTTTGTCTCAAAGTTCATTTAAAAGCAGTAAATAATTTTTACTTAATACCCCCCCCCCGCAAGGTCTTTGAAGTCTAACAACTTATCTCTATAATATTCGTATTGCTTTTTATGTTGATCAATTTCAAGAGGAAGGCCAGATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGGATTTCAACAATTTTATTTTGGATGGAAATGTGAGGGATAGGGATTTCAATATTTTCCATAATATTACTCATAAGTTTAGGATTTCCACTTGCCTTGTTTACAAATTTTTTTGTCTGCATAGATAAGCAAGTGCTTAAATATTTCACATTTAATTTTGATTTGTTTAGAACATTCAATATTCCACACACATTAGTTATTGAAAATCTACCAATTCTATAAAATACTGTCCCGGCATATGCTCCATCTGTAGTTCATGTTACATATTCACCATCAAAATCATATTTGTCAATTTTTCCTAAAATTCCATCGTTTAAACTTTGAGATGAATAAACAGGGTATTCACCTTTATTTAATTTTATGAATTCCTTTGAGTAAACTTTTCCTCGCTTTATTTCACACAAGTTTTTAATTAAATCATATTCAACTATATTGACAATTTTGTTTCAAAGTTCATTTAAAAGCAGTAAATAATTTTTACTTAATACCCCCCCCCGCAAGGTCTTTGAAGTCTAACAACTTATCTCTATAATATTCGTATTGTTTTTTGCGTTGGTCAATTTCAAGAGGAAGGCCAGATTGAATGTCCTTGGTGTATGTTTCAAGTTTGTCAAGAATTTCAACAATTTTATTTTGAATCGAAATATGAGGAAGTGGAATTTGCAATTCAATAATATTTTCCATATCAGGGTGTTTTATTGAGGAACCCCTAAAATTTTGTTCAATGTTTTCTTTGCTAGCAATTAAAAAATAATATAAGAATTTGAGACTATATTTTTTAACATCTTTTGATGATAATAATATATTTAAACTATCAATAAAATATCCTTTGTAGTATTTTATTAATGGCGCGCCACCAGAAGGTATTGAAATAATCTCACCATAATTAATGTTTTTATCATTTTCAACATAGGAGGTAAAACCATCATATTGACCTGTTGATAATAATTTTACATTTCCCGTTTCTTCTTTTTTATAGAGCTTAAGTTCATTTGCAGAAACATGCTTAAATGACAATATATTTTGTTGTTTGTTTTTATCTACTCCTTTAAATTTTTTGTCAAAATCAACAATTTCTCATAACCTCTTATATTCAACTATACTATTACTGCATTGTTGTTTATTCATTATCTTGTTTAATAATTCTTTATTATTATCAAAGTCTAATAATTTATTTCTATAGTAATTATATTGTTTATTTCTATAGGTCAACTCGGTGGTCAACTCGGTGGTCAACTCGGTGAACTTGTCTAAAATATTAACAATTTGATTTTGGATTTCAAGAGGGGGAATTGGAATTAAAATTCTTTTAAATTGCTCTTTTGATAAATTAAATCTAGTTACTCCATTAACACATTTAATTATTTCCTTTCTTATATTTAAATCCTTAAATAAATATTTAAAATAATTTACATTATAATTTTCGATATTGAATAGTCTATAACCAAAACAGAAACTATTTAAATAAACATCATTATTGTCGAAATATTTATCATTAGCTACAGAAGCGTAACCGCACTCATTTGCAGTTTCGGATGAAGTAGTAAAAAGAATATCTCCATATAACACTCTATTTTGCTTCTCATTTTTTAATACGGAAACTTTTTCTAAACTTTTAATATCAATAGATAAATTGTTAAAAATATTTAAATATGTAATATATTGTTTATTGGCATTATTCAAAAAATCATTTTTATTTTTGCCTGACAATCCATTATATAGAATGCCTAAATCTCCAATTTTTTTATATTCAACTCCATTAGGACATAGTTCCTTAATTAACTTTTCTATTTTGTTCATAACTATTCCTCTAATTCAGCAACTATTTTGTCAATTTCGCTTCTCAATGTTTCAATCTTGGAAACTGTTTCTTTTATTTGAGAGTTTAAGACATTAATATCTATTTTTTCTGAAGTGTCTTGTTTTTCAACGTAACTATTGACTGATAAATTATATGAATTGGACCTAATTTCTTCAACGCTGGCTAATTTTGAAATATAAGAAATGTCTTTTCTTTGGCCATAATAATCAATAATATTGTTAATATTTTGACTAGTTAATTTGTTGTTGTTTGTTACTTTCAAAAATTCTTGAGAGGCATCAATAAATAAGATATTGTTGTCAATTTTTGATTTTTTCAATACCATTATACATGTTGAAATACTTGTTCCAAAAAACAAGTTGCTTGGGAGTTGAATTATTGCATCTATGAAGTTATTTTCAACTAAATATTGTCTAATTTTTTGTTCAGCGCCCCCACGATACATAATGCCCGGAAAGCAAACTATTGCAGCTACTCCATCAGTTGCAAGTCAAGAAAGGCTATGAAGTATAAAAGCAAAATCAGCTTTGCTTTTTGGAGCTAATATTCCAGCAGGCGAAAATCTTTGGTCATTTATTAATAAAGGGTTGTCTTCACCTTCTCATTTAATTGAATAAGGAGGATTTGAAACAATAACATCAAATGGTTCTTTGCGTTGATGTTCAGGACTTGGAGATAGCAATGTGTCGCCGTTATAAATATTGAATTTATCAAATCCTATATCATGCAAAAACATATTTATTCTACATAAGTTGTATGTTGTTAAATTGACTTCTTGACCATAAAATCCATCCTTGATGTTTTCTTTGCCAAGAACCTTGATTGTTTGTAGAAGTAAAGAACCCGATCCACATGCGGGGTCATATACTCTATTAACTTTTTTCTTATTGAAAACTGCTATTTTAGTAAGCAATTCTGAAACTTCTTGGGGAGTAAAATATTCACCCCCTGATTTTCCGGCATTTGATGCATACATTGACATCAAATATTCATAAGCATCACCAAAGGCATCTATTGAATTATCCTGATAATTGCCTAATTTCATGGCTGCTATTTCTTGCAATATTGCTGCTAATTTTTTATTTCTTTCATCAACACTACGACCTAATTTTTGAGAATTAACATCAACGTCATTAAATAGACCTGAAAAATCATTTTCACTTTGTTGTCCCTTTGCTGATGATTCAATATTTTTAAAAATATTGTCCAACGTTTCATTTAGACAATTATTTGTTGTAGCATTTCTAACAACATTAATAAACAATTCAGAAGGTAAAATAAAAAATCCTTTTTCATTGATTAAATCATCTCTTCCAGTCAATGCTTCTTCATCTGATAAAGTTGTATAATCAAAATCTTCAATTCCTGCTTGACGTTGGCGATTGTTTGCATATGTTGCAATATTTTCAGAAATATATCTATAAAACATAATTCCTAAAACATATTGTTTAAAATCTCAACCATCAATTGAACCGCGTAATCTATTTGCTATATCTCAAATTTTTTTATGTAATTCGTCACGTTCTTGATTATTAATAGCCATAATTTTTTCCCCCCCAGTGTAATAAATAACTTTTATATATAAATTTTACAAAAATAACCCCAAAAAAGGTTTATTATCACTTTAAACTTAAAACCTTTTGAATCTTAAATATACGATGACAATGTTTCTACAAATTAATATACAAAAAAACTCGCAATAAATGCAAGTTTTTTTAATATATCAAACATTTTTTAATCAATAGGTTTTATTGCTTGAAGAACTAATTTAGCAAGTTGATTGCCTCTCAAAACTTCAATAACAGCTAATGCAAAATCAAAAACAGCAGCCGCGCATTTTCCTGTTATTAATTTTTTATCTGGGCTTATTGATACTAATTCTTTACTACGATTAGATATTTCTTTTACACTGTCGTTTATTGGAAATGAACTAAATTTAGTTTCACTATCAATAAACTTATTTTCATATAAAACATTGGGAGCATCGCATATTGCTATAATTAATTTGTTTTGATTTTTAAACTTAGCTATTAAGTTTAAAAATGTTTGGTTCTTTCTAATACTTTTAGCAGCGGGTCCTCCAGGAATAAATAAAACATCATATTCATCAACATTTATATTTGAAGTTGTTTGCAATTTTACAATTTTATGTGCACCAGTTGCTTCAATTCCTAGAGGATTGTAAAATGTGGTCTTATTAAATAAATTATTTGCTCTATGAAATAAATCTAATGTTCCTGTTAATTCAGTATCTTCAAATCCTTCTTCAACAATTACTAATAAATTCATAATTTTCACCTCTTGTTTGATTATATACTTTTAAACAAAAAAGAATAGATAGTTTTAACTAAAAATCTTTTATTTTGTTAAATAAATTATTTTAAATTTAAAAAATTCAAGTGGAATATTTTTAACACTTGAATTTTTATATTTTTTAACATTTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGATTTTGAATATTTTAAGCTAAAATCCATTTCAATTGTTTTAGGATTTGAATTCATATGAATTGTTAATTTATGAATATTAATATATAAACCAGGCATTTCTTTTCAATCTTTTGCAGCACCTAATTCAAAATTTTTAATTGTGATATCACCACTTTTTAAATTTGCATAAATTTTAGTTTTAGAGTTGCGATATAATGAATTATATTTAAAGAATGCTTCTTTTAAATCAACATTTTTATTATCAATAAATTTTGCTTCGAGTAAAACTTTTTTAGGAATCAAGAAAAAAATACAATTAAATTCTTTATCTTCTAAGGTGAATCTATCTACAATTCAATAGTCTTTTCAATATTCAAATCATTTTGAATCTGGCCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCTGCTATACTTTTATTGCTTTGCAATACTGAATTATATGATTTGGCACTGAATAAATTTTCTATATAAAAATGTGGATTACTATTTCCAATAATGTTTAAAACTTTTTTATGTTGTTCTTGTTCATTATAGCAAGATGTAACACATAATGGCAAAATTGGTAATGTTGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATCATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTTATAAAAATCATAAGGATCTTTATTTAGTATCTTCTTCAAAAATAAGTTTAAATTTTGAATATGGACCAGTTTTAAAATCATATTTGCTTTTTGGGTGTTGAATATATCATAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGACTGCAATCTAACATTAATTGTTTAATGTAAAAAAAGCGCAACTCGGGTTTACTATTCTTATCATCTAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTTGAAATTTTTCTAGGTTGTTCATTAAACACGAAATTAAGGTTTTTACTATAAAAGTCTGCTAAATTAAAATCTTCGCTTGAAATAAATCCTGTTTCTAATAATACCTTTTTAGGAATTAAAAACAAAATGGAATATGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATGGTTGCGTCGTTATTTAAAACTGATTCTTGTTCTTTTAAAATATCAACCATATTAAAAACTATTTCTTTATTGCCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAGGTTACAAATAATGGCAATATTGGTAATGTAGAAATCCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCGTTCCCGAATATTTAATTCCGTTTTTAATAAAATGTTTCCTTGTTTTATAAACTTTTCCATTTGGCTTCATGCCCAATAAAAATAACTGACCAGAAAGACTTGAAGAAACAATTACTTGAGAATATCCTCCCCAACCATAATGGCAAAGAAGTTTATTTCCATTGTCATACATTCCATAAGCAACAATTGCATGACCAACTTTTCCTCTCCCCATCGGATCAGGCATATATGTCCCAAATATTAAACAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCCGCAGATCTTCGATAAACATAAACAGGTCAAAAATTTGGTAAATCTGGTTTTTTTAAAAAATCATAAGCAACGTTCTTTAAATATGATGAGGTTGTTACTATTGCTCCTCATCCGTGTTTTGTCCATAAATCTGTAACAAATTCATCAGAAATAGCAGGAGAATATGGATAGCCATATTTTTTAAATGTTGAATAATCCGATGAATCCTGATAATCTACAAAGTATTTTTTCACTTGGTCATCTGAAAAGACTCCATAATTTTGAAAAAACTCAGCATATAATAAAAGCATACCTAATGCAACATAATGACAAAGACCTTGGTGTATATCAGAACATTTATATTTGTCTATTTTGTCTTGAATAAATTTTGCTTCTCCCTCATCGCCTCTTAGTTTAGCTTCCTCTTTTTCTTTTTCTCATCTTGCATTAATTGTAGAATAAGATTGATAACCAAAATTTTTATCTTTTATTGCTAGCTTAAATCATCAAGAATACGGAACTTCTTTTTCCGCATGTATCATTTCAACATCAGTATTAATTTCATGAGCCTCGCTTGAATTTCTATAAGAAATAGACTTTTTTAGTGAAAACTTTAAATTTTGTTTTTCTTCTATTAATTTTTTATTATTTAAATTATCACTAATGTCGTAATATTTACCTTTGAAATTTTTTAAAACAAAATTTTCTTGATAAGAACGAATTTGAGAAGAGTTATTTTTTGGCTGAATTCTTAAGGAATCTAAGTCTAAAAAATATTTTTTATGAAAATCGCTACTGTTAAAATTTTCATTTTTATTCAAAGGATCAATGATGACACTTTCATTATTTATTAACGAAACAACCATATATCCGTAAGGATTAAAATTAACATAAATATATGGATTACCAAAATTGTCAAAAATAATTTCCATATCTTTTATTTGATAATCAATTTTCCTATTGTATTTAAATATTTCCAATTCCAAAAACTTTAAATAAGCTTTTACATCAGAAATATTATATTTTTTTAGTTCATTTATTTTTTTTGCATTATTTTGAACAAATTTATTGAAATTGTCAATTTTTTGCATATATTCAATTAATTCAAACATTTTATTCCCTCTTTGTATATTTAATTAACTTATATTTTTATTATACTTTATACAAAGAAACCCCCTTTAAAAATGTTTCATAAAACCAGTAATCTAATAAAAAATAAGTAATTTTGGTTTTTTTATTTGAAATCCTTTTTTAAATATTTAATTTATTTTTAGTAATTTTTTTTGGTTCTTGTAAAGATTATAATTCATTATAAAAAGAATTTCTTGCATTAATTTTTTGAAACCTTCAACAAAAAAATGGCCGGTGAGAGCCATTTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGTTTTAAAAGAAACTTTTGCAACCTCGCGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGTGCCAAGTTTGAATTCAAAATTATATTTTTTATTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGAAGCTGCTGCAACAGCCAGGGATTTTTCTTCGCCTTTTGTTTCAATAATTATTTCATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAATTTTTTGAAATGCTTTTGCAACATTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGATTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATTAATTTATTATAACCACTTTGACTAAGGTAAGTTTTTTGATAATAAACTAAATTTTCAATTTGATTAAAAATTTCAATTATTTTTTCATGAAATAACCTCAAAAAATTTGTTATCACTTTAAATTTAAAAAATTCAAGTGGAATATTTTTAACACTTGAATTTTTATATTTTTAACATTTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGATTTTGAATATTTTAAGCTAAAATCCATTTCAATTGTTTTAGGATCTGAATTCATATGAATTGTTAATTTGCGAATATTAATATATAAACCAGGCAATTCTTTTCAATCTTTTGCAGCACCTAATTCAAAGTTTTTAATTGTGATTTCACCGCTTTTTAAATTTGCATAAACTTTAGTTTTTTTAAAGTTCGAATATCCTGAATTATATTTAAAGAATGCTTCTTTTAAATCAACATTTTCATTATCAATAAATTTTGCTTCGAACAAAACTTTTCTAGGAATCAAGAAAAAAATAAATTTTTTGGAATTGGATTCTTTATCTTCTAAGACTAATCTATCTACAATTCAATAGTCTTTTCAATATTCAAATCATTTTGACTTTGGTCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCTGCTATACTTTTATTGCTTTGCAATACTGAATTATATGATTTGGCGCTGAATAAATTTTCTATATAAAAATGTGGATTACTATTTTTAATAATATTTAAAGCTTTTTTATGTTGTTTTTGTTCATTATAGCAAGATGTAACACATAATGGCAAAATTGGTAACGTTGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATCATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTATAAAAAACATAAGGATCTTTATTTAGTATTCTCTTCAAAAATAAGTTTAAATTTTGAATATGGACCAGTTTTAAAATCATATTCGCTTTTTGGGTGTTGAATATATCACAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGACTGCAATCTAACATTAATTGTTTAATGTAAAAGCGCAACCTGGGTTCACTATTCTTATCATCTAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTTGAAATTTTTCTAGGTTGTTCATTAAACACGAAATTAAGGTTTTTACTATAAAAGTCTGCTAAATTAAAATCTTCGATTGAAATAAATCCTGTTTCTAATAATACTTTTTTAGGAATTAAAAACAAAATGGAATTTGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATAGTTGCGTCGTTATTTAAAACTGATTCTTGTTCTTTTAAAATATCAACCATATTAAAAACTATTTCTTTATTGCCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAGGTTACAAATAATGGCAATATTGGTAATGCAGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCGTTCCCGAATATTTAATTCCGTTTTTAATAAAATGCTTCCTTGTTTTTTTAATTTCGCCATTTGGTTTCATACCTAATAAGAACAATTGGCCAGAAAGACTCGAAGAAACAATAACTTGAGAATATCCTCCCCAACCATAATGGCAAAGAAGTTTATTTCCATTGTCATACATTCCATAAGCAACAATTGCATGACCAACTTTTCCTCTTCCCATCGGATCAGGCATATATGTCCCAAATATTAAACAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCTGCGGATCTTCGATAAACATAAACAGGTCAAAAATTTGGTAAATCTGGTTTTTTTAAAAAATCATAAGCAACGTTTTTTAAATATGATGAGGTTGTTACTATTGCTCCTCATCCGTGTTTTGTCCATAAATCTGTAACAAATTCATCAGAAATAGCAGGAGAATATGGATAGCCATATTTTTTAAATGTTGAATAATCCGATGAATCCTGATAATCTACAAAGTATTTTTTCACTTGGTCATCTGAAAAGACTCCATAATTTTGAAAAAACTCAGCATATAATAAAAGCATACCTAATGCAACATAATGGCAAAGTCCTTGTTCTGTATCAGAACATTTATATTTGTCTATTTTGTCTTGAATAAATTTTGCTGTTTCCTCATCTTTTCCAGCTTCCTCTTTTTCTTTTTCTCATCTTGCATTAATTGTAGAATAATCTTGATAACCAAAATTTTTGTCTTTTATTGCTAACTTAAATCATCAAGAGTACGGAACTTCTTTTTCCGCATGTATCATTTCAACATTAGTATTAATTTTATGAGCCTCGCTTGAATTTCTATAAGAAATAGACTTTTTTAGTGAAAACTTTAAATTTTGTTTTTCTTCTATTAATTTTTTATTATTTAAATTATCACTAATGTCGTAATATTTACCTTTGAAATTTTTTAAAACAAAATTTTCTTGATAAGAACGAATTTGAGAAAAGTTATTTTTTGGCTGAATTCTTAAGGATTCCAAGTCTAAAAAATATTTTTTATGAAAATCGCTACTGTTAGAATCTTTATTTTTATTCAAAGGATCAATAATGACACTTTCATTATTTATTAACGAAACAACCATATATCCGTAAGGATTAAAATTAACATAAATATATGGATTACCAAAATTGTCAAAAATAATTTCTATATCTTTTATTTGATAATCAATTTTCCTATTGTATTTAAATATTTCCAATTCCAAAAACTTTAAATAAGCTTTTACATCAGAAATATTATATTTTTTTAGTTCATTTATTTTTTTTGCATTATTTTGAACAAATTTATTAAAATTGTCAATTTTTTGCATATATTCAACTAATTCAAACATTTTATTTCCTCTTTGTATATTTAATTAACTTATATTTTTATTATACTTTATGCAAAGAAACCCCCTTTAAAAATGTTTCATAAAACCAGCAATCTAATAAAAAATAAGTAATTTTGGTTTTTTTATTTGAAATCCTTTTTTAAATATTTAATTTATTTTTAGTAATTTTTTTGGTTCTTGTAAAGATTATAATTCATTATAAAAAGAATTTCTTGCATTAATTTTTTGAAATTTTCAACAAAAAAATGGCCGGTGAGAGCCATTTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGTTTTAAAAGAAACTTTTGCAACCTCGCGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGCACCAAGTTTGAATTCAAAATTATATTTTTTGTTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGAAGCTGCTGCAACAGCCAGGGATTTTTCTTCGCCTTTTGTTTCAATAATTATTTCATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAATTTTTTGAAATGCTTTTGCAACATTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGGTTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATTAATTTATTATAACCGCTTTGACTAAGGCAAGTTTTTTGATAATAAACTAAATTTTCAATTTGATTAAAAATTTCAATTATTCTTTTATCAGAAAGTTTTTTGCTGTCTTTAACACCAATTTGTTTTAGTTTTTCAATGTTATCTTTTGGTATATAACAGGCAACTGAAATCACGGGACTGAAATAATCTCCTACTCCCGTTTCATCAACACCAATTAATTCATCTAAGTTTTTATTCAATTTCTTCTTCTTTACTAAAAAAGTTATCTGCACCTACAGTTTTAATATTATTTTTTTGTTCGGCCTTTTGTTTTTTACCTAATTTAATGTATTTTTCTGGGTTTTTTAGGAATTGATCAATAAAGTATCCGACTGCGCCTTCTTTATTTGTTTGCTTCATAACAACTGTTGACATCTTTTTAATTAAGGGTTCAGCATTCGCAGGTGATACACAAACATTAGCAACATTATACATGCTTAAATCATTATATGAATCTCCCATTGCAACAGTATCATCAATGTCAATGTTGTAATATCTCATAATTAGTGAAATAACTTTTCCTTTATCAATACCTATTGAAGTAATATCAAATACTGGGCTTAATCCTTCACCTTTTGATCAAGAAGAAAATTCTCCTAAATCTCCATAACGTCTTTTTAAATATGTCAATAATTCCAAAACATCTGTATCAGGTTTAACATCAAATACAATTCCAGTTGGTTTTAGTGGAATTTTTTCTAGGTTAATACATTCTCTAAATTTTGTTGCTTGGTTAAATCCAAACACTCTTTCAAGGTTAGGATCTCTATGCATTAATTGAACCCAGTCAGGGCCTTCTATTGCATAATTAGTAATTTCTTTTTTTACTTTTTCATCGCCTAAAATATAAAGAACTTCATTTAAGTCCAAATATGTAATAGCAGGTATAAAGAATGGGTCAGCAGGGTTGTGGATGTGAGCACCATTGTAATTACCTACAATTGCATTAAGTCCAAGTTTTTCATACACAGGCAATGTACTTCTTCAAGGTCTTCCTGTAATTATTGAAACAATGTGTCCTTGGGCAACAGCTTTTTTAATTGCTTCTTCTGTTTTTGGATGAACTGTGCCATTGGCTGAATCTGCTAATAGTGTTCCATCTAGATCAATAGCAAATAAAAATCTTCTTTGTTTTGATTCTTTGTTGTACGCCATAATTTCCTTTCATAATATATAAATATTTTACAATATTTTTTATATCTTCTACATTTTGAACAAAATTACTAAAAAATATGTCTCAATTTTCAATGTTTTATTGCAAAAATTTACACGGAATTAAATTTATTGCAAAATAAAAACTTCAAGTTTTTTTACTTGGAGTTTTTTAAAAATTTATTTATTTTATTTTTGTTCCAAATATACGGTCACCAGCATCTCCCAAACCAGGCAATATATAATTGTGTTCATTCAATTTTTCATCTTTTGAAGCTAAATATATATCAACATTAGGAAATTGTTTTGTTACTTTATCAATACCTTGTTGAACGCCTACTAAACATACTAATTTAATATTGCTAAAGCCTAACTTATCCAATTTTGCTATTGCATCGCATGCAGATGAGCCGGTTGCAAGCATAGGGTCAACAATAATAATATAGCTATCATGTGGTACTTCTGGAATTTTAAAGAAATATTCAACAACTTCGTTTGTTTCTTCGTTTCTATACATTCCAATATGACCAACTCTAGCTTGAGGAACTAAATCCATAATACCATTCAACATTCCAAGCCCCGCACGTAAAATAGGAATAATTACAATTTCTCTATCAAGAGTTTCACCTTCATATTTAACATTCATTGGTGTTGTTATTGATATTTTCTTAGTTTGATAGTCCCTCAATGTTTCATATACCATCAACGAAGCAATCTCGTTCAAATTATCTCTAAAATCTTTGTAAGATGTTTCTTTTGAGCGCATCGTTGTTAATTTAGCATTAATTAAGGGATGCTGAAAAATTTTTACCATATATTTGTGTTATCAACTTTCTTTTTATTATTTTTCTAATTTAAATAATTCTTCCATAACTGGAACTGATTTAGACATACCTAAACGGTTTGCACCTAATCTAATCATTTCTTGGGCATCTGCTAGATTTTTAATACCACCTGATGCTTTAATTAAAAGCTTGTCACCACAGGTTTCCTTCATAGTTTGAATGTCTTCAAATGAAGCTCCTCTATATGAAAATCCAGTTGATGTTTTAATAAAATCAGCGCCAGATTGCATTACTAATTCAGTAATTTTTATTAATTCAGCTTTTGTTAACAATGCTGTTTCAACTATAACTTTTAAAGTTTTGCCATTGCATGCTTCTTTTATTGCTTTGATTTCGTTTAATACGTATTCATATTGTCCATCTTTAAATCTTCCAACATTGATTACCATATCAATTTCGTCAGCACCATGTTCAACAGCAATTTTTGTTTCATATACTTTAACACTTGTTGCATTATAGCCTAGTGGAAAACCAATAACTGTGCAAACAAGCACATCACTGTTTTTTAATGCTTCTTTAGCATACTTAACATATGATGGTGCAATACATACTGATTTAAAATCATATTTAATTGCTTCTTGAATTAATTTATCAATGTCTTTGCTTGTTGCAGAAGGTGATAAATTTGTGTGGTCAATATATTTGTTTAATTCTGTCATATTCTTCCTTAAGTTAATTTAATTAATTACATTAATTTAGCAAAAACCGTTTGAATGTCATGCTTGCTTTCGCTAAATTCTATTGCAGACAACAATTCTTTTTGAAGTTCTGGATTTATAGGTTTTGACGAATACAATGTAAATAATACATCTCCTTTATTTACATATTCATTTGAACTCTTATTCAAATAAATTCCTGCTTCATAGTCGATAGAATCTTCTTTTTTAGATCTACCTGCTCCCAATTTCATTGCTACTAATCCAAAATCAATTGCAGATTTTATTTTTAAATATCCACTTTGGCTTGCAATAATTTCTTGTTTATATTGAGGATTAAATCATTTTGAATCTTTTTCAAAAACCTTGATGTTTCCGCCTTGTGCTGAAATTCATTCCAAAAATTTGTTATATGCTTTTTTATTATTGATTACTTCATCAATCATTTTTTTTGCTTCAACTTCATCTTTAGCTTTTTGTGCCAAAACTAATAATGTTGAACCTGATGAGTAAATAATTTCTGTAAAATCTTTAGGTCCGTGTCCATTTAATGTATCTATTGCTTCCAAAACTTCAATTTTATTTCCAATAGTTTTTCCTAAAGGTTGTTGCATATTGGTTATTTCAACGGCAATCTTTCTATTTAATTTCTTACCGATTTCAATCATCAATTTTCCAAGTTTTTTTGCTTCATTAATATCTTTCATAAATGCACCATTGCCACATTTAACATCCAATAAAATGCAGTTTGAACCAGTTGCAAGTTTTTTAGACATAATTGATGAGGCAATCAATGGAATTGATTGAACGGTGCCAGTTACATCACGTAATGCATAGATTTTTTTATCTGCAGGCACAAGTTTTTGATTTTGTCCAACTATTGCAATACCATGCTTTTCAACTAAGTCTCTAAATTGTTGGTCAGTCACTGAAAAATTATAACCGTCAATTGATTCTAATTTATCAAGTGTTCCTCCTGTATGTCCAAGTCCTCTTCCCGACATCTTTGCAACAGTTAAACCTAAGGCTGCTAAAATTGGACATAATGCAATACTTACTTTATCTCCAACTCCTCCTGTTGAGTGTTTATCAACGATTACTTTATTTAAATAACTTCAATCAAGTACTTCGCCTGAGTGCATCATAGCGTCGGTTAATTGTGCTGTTTCAGCATCGCTTAAACCATTTAATCTAATTGCCATTAATAAAGCAGCAGCTTGGTAGTCAGGAACATTTTCTGCTACATATTCTTTAATGAAAAAATCAATTTCTTCTTTGGTAAGAGTTTGATTTTCTACTTTTTTGTTAATAATATCAATAATTCTCATAATTATTTCTTTCTATTTATTCAAAGTCTTCTGATATTGATAATGCTAATTCCATCATTTGATTGAATGAATTTTGTCTTTCTTCAGCAGTTGTATATTCATGTGTTATTAAATTATCAGAAATAGTTAAAATAGTTGCAGCTTTTTTGTGCAATTTTTCTGCTACTGTAAATAACCCATATGCTTCCATTTCAACAGCAACTGCTCCGCCTGAATTTTTAACTCTATCTTCAACAGAAAATACTGAATAAAAGGCATCTTCCGATAAAATTCTTCCTTCTTCAAGTTTTAAATTCAATTCCTTAGCATGTTTTTTAATTAAATCATTTAATTCGCTATTTGGAAGCGCAACATTACTTTCATCATTGACTAATAAATTTTTACGGAATGAAGTGCTATCACTATATGCTGAAGTTGCCAAAACTAATGAATAATTTTTTAAATCAGCCTTGAATGAGCCACCTGATCCAATTCTAATTATTGCTTCAACTCCATATTGGCTAAATAATTCATATGAATAAATTCCAATTGAAGGAACGCCCATGCCTGAAGCACAAATAGAAATTTTTCTTCCTTTATAAAAGCCAGTATACATTAAAACATTTCTAACTGTTGACACTAATTTAGCGTTTTCTAAATAGTGTTCAGCAATATACTTTGCTCTTAAAGGATCACCTGGCATTAAAACTAATTTTGCAAAATCATCATCTTTTGCATTTATATGAGGTGTCATATTTTCTCCTTATTCTATTGTTAGTTTTTTATACATTATTATTTTATAATAATTAAATAATTAATAAACATTAAATGTCTAAAACGCTAATAAGTCAATACATAAAAAAGCAAAAAGATTAACTTCTTGCTTTTTTATGACACTATAAACCGCTTTCTGTACTATTTTAATAGCGCCAACTATTTATCTAAGCCAATTGGCTTCCGGGTTCTTTGTTCGGTTCCTTGTTCCCAGTTCCCCTACCAAAATTTGGGTTTCTAGCTCATGGAGTTTACATCGTTGCATCTTAACTAAATAAGCTCGTCTCTGTTGCACTAGTCGTCAACTAAATTGTTGCCTAGACTTATTGGGTCTAGCATGAACACTACTCGCATCACAGCGAGTGCTAGAGCGGATTTTCCTCTACTTTGTAGTAGCAGTTGGCCATGCAATTTAATTTTACCTTAAAACACAAAAAATATCAATGTTTTTTTAGTAAAAAAATATAATTTTAATATATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATGTTAATGAATTTGATGAAATAATATCATTACTAACAAATAAAGGCATTCAAAGAATTTTTTGCCCCGGGGTAAATAAAACTAATTCAAAAAATGGCCCCTCAATTTTAATTTTAAACTTAGTTAATTGCGAAGTCATTAAATCAGAAACAAGAAATTTATTGCCTAGACTAAAAACTGCTTCGTTAATAAAAAATTTTCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAATTGTCTTGACAATTGCAATAATATTAATCTTATTAAAGAATATGGCCATTGCTTAGATTTTATATTGTCAAAATCTGATAAAGTTGAAGCATATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCTAACTTAAATTCTTGTATTGAATGTAACAATGTTGAAAATATTGTAGATTTTAAGCTATACAAAGGGGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCGTTATTGAGGTCTTGATATTGAATAAATAAAAAATTTGAACAATTTGATTTAAATTGTTCAAATCTAAATGCTATTAAAATCAATAATGAACTTAAAATGTTTTTGTATGATAATAACATTATTTATCGTTCATAGATATTTTTTTTATTCCATCAAACTTACGTTGCAATGTTTTAAAATCAATGTTATTTGTTTTATTAGAACGTAACATGTCTTCAAAATCATTTAATTTTATTAAATCTTCCTTTGATATATTCTTTTTATGTAAATTAATAATTAAATTAAATGCTTCATTATAAAATAGTAAGTATTGCTTAAAATTATCTTCTAAATATTCAAACAGATTTTTAAAATCATCAGAAATTTTAGCCAATGTTTCTTTGTCTTGTTTATCTTCAATATATTGACTAAAAATATGTTCATTTTTATCCACTATTTTTTTCTTAAAATCATATTGAGCAACTCTTTTTTCAAAATAATCTCTTAAGTTTCAAAATACACCATAATCAGGCAATGAATGAATTAAATTTAATGAACGTAAATATTGCTTATAAATTTCTTTACGAATTATTATTAATTGACTGTTTGAATTAGTTTTTTTATGATTATTAACAAGAACATAAGTAATACCTAGTAAAAAAGGCAATCCAATAACTGCACTAATTCCTGAAATGATGCCTATTTTTGATCTTCTAGTTAAAAATCTATGTGGTTTTTTATTTTGAACTTTTTCTTGATTTTTTGATAATTTCATAACAAAATTATATAGTTTAAATTAGTATTGTTATTTTAAAAATAGTAGAAAAATTTATTATTTTTTAGTAGATTTTGCCAATAATATAACATTTTCATCAAGGTTTGGATCTTCTTCAATACTTGCAGTAGTATGATTTGTAAACAAAATTGTAGGAATAATAACAACATCTCTACGTTTATTTTTATAAAAAAATGGGGCTAATCTATCAATTATTAGTTGCTTTAATTGAGGCACAGTTCAATCATTTGAGTTTTTAATTGTATATAATATAGCGCCATGAACCAATCTCTTAGCTTCTTCAATTAAATGCAATGAATTTTTTACATAAAATGCACCACGAGAAACTATTCTTGGTTTACCAATAATTAGATTCTTTTCTTTATTAATTGCTAAAACAACATGCATAAATCCATTTTGGCCTAATTCACTACGTTCTTTAATAATTTGAGAGTTAGTTTTTGATAAAATATTGCCATCAATAAAAACAGGCCCGAAATAAACCTTTTCGTTTGACAAACGAACTTGATGATTTTCTAAATAATAAACATCCCCTAATCTAGCAATTAAAATATTTTTAGCATCAACACCGCTTTCAATAGCAGTATAGCCGTGAACAACGCTCATTCTATATTCACCATGATAAGGCATAAAATATTTTGGTCTAGTCAATTCAAAAATTTTTCTATGTTCATCTTTGTAGGCGTGGCCCGAGGTATGTAAATAACCGTCTACTCCATTTTCACGAATTATTGCGCCTAATTTATATAGTCTATTAACTAATAATTCAATTTTAGATCTATTTCCGGGAATTGGGCTAGATGAAAAAATAATAGTGTCTTTTGGTTTTATTGTAATATTTGGATGTTTTCCTCACGACATCTTTGCAAGTCCAGCTAGTTCTTCACCTTGAGAACCAGTTGTTAAAATTAATAATTCATTATCTCCAAATTTACTTAATGATTTTTTGTCAATAAACACTTCGTCTGGAACATTAATATAACCTAGTCTTCTTCCAATATCTATTCCATCAACCATAGAACGGCCGAAGGCAACAACCTTTTTATTTAAAGAAGCACCTAATTCAATAATTGCTTTAATTCTAGTCAAATTAGAAGCGAATGCAGTTACAATGATTTTCTTTTCCGCTTCCAACATATATTTTTTAATATCTTTTAAAATATCTGACTCTGATGGTGAATGATTTGGACGCATAGCATTTGTTGAATCAGAAAATAAAACATCAAGGCCTTCGTCGCCAATTTGTTTAAGTTTATTAAAATCTGTAAAATTTCCAATAGGTGTGTAGTCAAACCTAAAATCACCTGTCATCATTAATGAACCATTAGGGGTTTTAATTCTAATGCCAAAGGCATCAGGAATTGAATGTTGGGCAGTTCAAAAATCTACTTGACAAGGGCCAAAAGTTCAAACTGCATCTTTTTCAGTTTCAATAAATTCAATATTACGTTTTATCTTCATATCCTCAAATTTTAGTTTTAGATATTGAATAGCAATTCTAGGTGCAAATATTTTTTTAACATCAACTTGTTGAACTAAATATGGAACACCTCCAATATGATCTTCATGTCCGTGGGTTATGAAAAGACCTTCGATTTTATCTTGATTTTCTGCTAAAAAAGTGAAATCTGGAATAGAACCATTTACACCTGTTACATATGAATCGCAAAATTTAATTCCGGTATCAATAATAAAAATATGATTTTCATATTGAATAACCAAAGTACTTTTACCAATTTCTTGCATTCCGCCTAATCCAAAAATTTTAGTTGGGCTCATAAATCCTCCAATTTTTATAATTATTATAATGAATAAATATTCAGCTTTTTATTGTTAATATTTTAGCAAAAAAATACGAAATTAAAAAAAATACAAATTTTTTATTAAAATTTTAGATATGAATGATAGTAGGGTACAAAAAACAAAGATTCCTAAAGTATTAGCAAGTTTTAGTTTAATTTTAGAACTTATTGCATTAGCATTACTTATTTATGTTATATATAGTTTCTCTTCAATTTGAAAAGAAACACTTGCAGATATAAAAGGAATTATAAAAAATAATAAAGACAGTTTTGAAACATTTTGAAAGGAATTTTCTAAATATTGAATAGACTTTTATAAAAATAATAAGGTCTTAATTAGTGCAATTTTAGCATTGCCAATATTATTATATGTTTGCTTTATATTTAGTATGTTAGTAATAGCAAAAGCTAGTTGGTCATTACACAAAGTTCCAATAATACTTATTATTGTTACATTATGTTTGCCAATTCTATTATTATGTGAAATAGTTAGAATAGTATCACTATTTTTAATAGTAATAAAAAAGCAAAAAAATTTTAATAACCAAGTATATTATGGACCACAAGTTGCTAACATACAAAATTAATAAAAAATTCGTAATTATTTCTATTACGAATTTTTATTTATAAAATAAGAAAACAAAAAGAAATCTTATTTATATAATTTATCGATTTTATTGCTTATTGCTCTTGCAGCAATTGTCCCATCAGCTGTTGCTGTTGTAATTTGTCTTACATCTTTTACAATAACATCGCCTATTGCAAAAATATTAGGAATTTTAGTTTCCATATTTTCATTAACTTCAATAAATCCACGGCTATTTAATATTTCATCATATTTACCTTTTATAAATGCTGTGTTAGGTTTAAAACCAACATAAGGAAAAATGCAACTTATATTCATTTTTGTTTCTTTTCCATTTACATTAGCAATAATTTCTTCAACTTTTTCATTGCCCAAAATTTCTTTAATAGTTGAATTTTCATGAATATAAATATTGCTTTTAGACATTGCTTCATTGACAAGTTTTTTTTCTGCAATAATTCCATCACGAACAAAAATATGAACCTCAGAGGCAATTGATGCTAAATAAGACGATTCTTCGAATGCTGAATTACCTCCACCAATGATTGCACAAGGTTTATTTTTATACAAAGGACCATCGCAAATTACGCAATATGATACGCCTTTGCCTCTATATTCTTCAATGCCTTTTACGTCCAAGGGAACAATTTCTTTCATGCCGGTTGCAATAATTATTGCTTTTGATTTTATTGACATGCCATTTTCCAAAATGACTTCTTTTTCAATATCTGATATAGAATTAATTTCCTTTACATTACCGTAAATAAATTTTGAACCATTATCTCTTGCTTGTTTAAGCATGGTTTTAGAAAGTTCAAAACCACTTATAAAACTAAAACCTGGATAATTTTCAATTTTAGATTGAGCAGAAACTTTTCCTCCTGGTACTCCGCCTTCAATAAAAGCAACATTTATGTCATTTCTTGCTAAATATATTGCAGCTGTTAATCCTGCCGGTCCTGCTCCAATTATTAAAACATCATATAAGTTATTATTTTCCATATATTTCTCCATTACTAAAAAAGCAGCAAATTTATTAAGCTGCTGTTTTAGTTTCTATTTGATTTTTAGTAGTAGATTTTTTAAATGCAAATGAGTTTGAATTTTTAGGTTCATAAACTATAAAGTAAAAGAATTTTGAAAGCTTTTCGTTATTATATGTATTAACTTTATAAACATTGTAATTCAATTTTATAACGAATTCAAACATTACAGTCAATACCAAACCAACTATTATATATAAAGCAGAAATTACTTTATAAATAGTATAACTGCCATCACGTAAAGGTTCCATTCCGAAACGAATTATTCCATAATAAACAATATATAAACCACCAGTTGTTCCTGGACGTAATCAATTTCAATTATTCAATATTCAAACTAAGATAATATAAGCAGCTAAGTTTGCAATTGATTCATAGAAGAATAAAGGATAACGGTATTTTCCATCAATAAACATGTGATCCCTAATTAATTTTGGCAATATTTCAAATGCAACAGAGCCTTTTTCCATAACGCCACCATAAACTTCATGGTTTGCGAAGTTTCCCCAACGTCCAATTGCTTGTCCAATTAATACCGCTGGTAAAATTATAGTAATTGCTTTTTTCAAATCAATTCTATTTTTATTTTTTGAAAATAGAATATAAAGAATACAACAAATTGTAGCAGTTATGACACCACCATGTATTGATAGTCCACCCTTTCAAATAAAGAATGCATTATACCAAGGACCATCTGCCCAACCTTTATGTTCAATTAGTTGTTGAATAACGAATACAAATCTAGCTCCGATAATAGCAGTAGGAAGTGCTATAAATACTAAAATTGTTAGTTGTTCGAAAGTATATTTTTCTCTTCTTCAGAAAAAATAAACAGTCAATATAGCAGTGAGCATTCCTAACATCATTGTTAAGGAATAAACATTCATTGGGCCTAATTTTAATTGAATTCCTGCTCAATCTTTAACTTGCATTTTCCCTCCTTATAAATTTGCCACTTACATTAAAATATAATTATAAATTATACAAATCAAAAAAATTTTTTTTAATAATCACTATAAAAGTTTTGCTAAATATTGCCCCGTGTACGAATTTTCACACTTTGAGACCTGTTCAGGTGTTCCAGAGCAAACAACCTTTCCCCCTTCATCCCCACCATCAGGGCCTAGGTCAATAATATGATCAGCGCATTTAATTAAATCTAAATTATGTTCAATTACAATTACTGTATCGCCATTGTCTACTATTCTATTTAATACTATTAATAAATTAGAGATATCGTAAGGATGTAATCCGGTTGTTGGTTCATCTAATACATATAAAGATTTGCCTGTTGGCTTTTTTTGTAGGTACGTTGCTAATTTTACCCTTTGAGCTTCACCACCGCTTAATGTCGTAGCATTTTGGCCTAATTTAATATAGCCAAGTCCAACATCTAATAAAACTTGCAATTTTTCTTTAATTTGGCTCTTGTTTATAAATAATGCATAGGCTTTTTCAATTGTCATATCCAAAACATCGCCAATACTTTTACCATGATATTTTATTTCTAGCGTTTCTTGATTATAACGTTTCCCGTTGCAATGGTCGCATATCACATAAACATCGGGCAAAAAATGCATAGCAATTTTGATTACACCATCGCCTTCGCATTTATCACAACGCCCACCTGGAACATTAAATGAAAATCTTGATTTTGTATATCCTCTAATTTTACTTTCTTCGGTATTTGCAAAAATTTCTCTAATATCGTCAAAAACACCAGTATATGTTGCAGGATTTGATCTAGGAGTACGCCCGATTGGATTTTGGTCAATTTGAATTAATTTATCAATGTTAGTTTGACCAATTAATTTACTATCTCTTTGAATATCATAATATTGATCTAATAAATATTTATTAAGACTTCCAACTAGTTCATCGTTTATTAATGTAGATTTTCCAGAACCACTAACTCCAGTAATTGCAATAAATTTTCCTAATGGAAAAGTTACTTTAATATTTTTTAAATTATTTAATTTTGCATTTTTTAATGTTAATGTCTTGCCATTTCCACTCCGCCTAAATTTTGGCACCTCTATTTGTCTTTCTTTTGACAAATATTGGCCAGTTAGACTTTGCTTACATGCAATTATATTTTCAACATTTCCACATGCAACAATATTTCCACCATGTTCGCCTGCTTGAACACCAATATCAACTATATAATCTGCTTGTCTAATTGTTTCTTCATCATGTTCAACAACAATTAAACTATTGCCTAATTCAACCATTTTTCTTAAAGAGCTTAGCAATTTTTCATTATCTTTTTGATGTAAGCCAATTGAAGGTTCATCTAAAACATATAAAATGCCTGTTAGATTAGAACCAATTTGTGTTGCTAATCTAATTCTTTGAGCTTCACCACCGCTTAATGTCTCAGCTTTTCTATTCAATGTTAAATACTTTAAGCCAACATCAATTAAAAATTTAATTCTATGTTTTAATTCAGACAAAATTAAAGAGGTAATTTCTTTTTCTAAATCACTTAACTGCAATCTTTCTAAAATAATTGCTAAATCTCCAAGCGACTTATTGCAAATTTCGTATATATCTAGATCTAGAATTTTGCAAGACAATGCATATTTATTCAAACGCTTTCCATGACATGAAGGGCAAGTAATGTCTCCAATGTATTTGCTATAATAAGCCCTAGCTTCTAGTGATTTTGTATCAAAATACTTCCTTTGTAATTTATCAACAATACCTTCAATAACTTGAAACTTCTCGCGTTTTGTGCCACTTTCGGATAATAACACATATGATATTTCTTCTTGCGACCCATGTTGAATAATATCTAGTTCAAAAGAAGATAAAGAATCAATAGGTTGATCTTTATCAATATGATAATAATTTAATAGCTTGTCAAATTCTTGCCATTCTAAATTGTAAGTATTCATGAAATTTCTATAATACTTTATTGCTCCTTCGTTTATAGAAAGCTGCCTATCGGGACAAATTAAATCAAAGCTTGCCTTTTGAATAACGCCAAGTCCTTTGCATTCAGGACACATTCCAGCTGGAGAATTAAACGAGAATAATTTAGGTTCAATTTTTGGCATATCAAAGTCGCCAAAACTGCAACTATGATGTATTGAATAGGTATTCTTTTTGCTTTCATTAACAATAGTTGAAATTAAACCATTAGATTGCTCCAATGCAACCTCAATTGCACTTGATATTCTTGATTTTATATCATTATCATTTGATAATTTAACTCTATCAATAAACAAATCAATATTTCAACGTTTATTTTTATCTAGATTAATCGAATCAACATTTTCTAAGAAATAAATCTCATCATTTATTAAAACTTTTATAAAGCCATCTCTTTTTAGTTTATTAAGTAATACTTGGTGGCTTCCTTTTTGGTTATTTACTAATGGGGCTAATATGTATATTGTTGATCCTAATGGGTTTTTATAAATGTTAGAAATAATGTCTTTTGTTTTTTGAGCAGTAATTTCAATTTTGTGATTTGGGCAATAAGGTTTTGCTACTCTTGCATATAGCAATCTTAAATAATCATAAATTTCAGTAACAGTACCAACTATACTTCTAGGATTAACATGTACTGTTTTTTGTTCAATAGAAATTGCTGGGCTCAATCCTTCAATGCTATCAACATTGGGTTTTTTAGTACCACCTAAAAATTGTTTTGCATAATTAGAAAGGCTATCAACATACCTACGTCTGCCTTCTTCATAGATTGTATTAAATGCTAAAGAACTTTTACCAGAGCCACTGACACCAGTAAAAACCACAAATTTGTTACGTGGAATTTCTAAATTAATATTTTTTAAATTATTTTCTCTAGCACCTTTTATTATTATTTTATCGTTCATTTTCTTTGATATAACCCTTTTCTATTAAATATTGTTCTGCTTTCTTTATTCTTTTTTTAAAAAATTTATTAATGAATTTTAAGTATTTCATATCCATTTTAATCTTGTTGGGTTGCCTAAATTTAAATTTCTTAAGTTGTTTAATTCAATCTAAATGTCTTTTTCTAATAAAAATTTTTAGTTGTTGATTAAAACTTTCAAAAAACCTGGTCTTGCGATTCTCATAGTCATTTAAAAATGCAGTTTTTGCAATTGATTTTAAATAAATAGGATAATCAAAAATTAAACTATTGTTGAAATCAAATATTGGAGCTGGCTTTGTTAAAATTCCGCTATTATTATCCAACATCATGCCAAAATTATTTAAATGCCTATCCCCATTACAAATTAAAGCATCAAAAACCATTAAATCTTCAAATAACTCTTTGCCATAATAAAAAGCAATCTGACTACATAATTTTAAATTATTACCATTAATATTGGGATCTATGATTTTAGAAAATGGGATGAATGAAAAATCTTTGTTTGTAAATAATTTGCAATCACTAATATAATGCTTTCCATACTTAGTAATTTTATAATCAACCGCCGGCAAATTCAAAAATTTGGCAACTTGACTTGCAAAATGTTCACTATAAACATCGTATAAGTTTTTATTTGCAAATTTTGAGTTTCGTTTATATAAATGAATTTCGCCATCCACCTCAACTCATGCTTTTAACATGTCGCCATCAATAAATCAACTAGGGCTTTTAATTTGTAGTCTGTTAGTTTCTGTTAATTTTGCTTTATAATCTAAAAAATAATTGCAAGAATCATTATTGAAATGATCAAAATAATTTACATCTTCTCAATTTAATTTATTGCTTTCTTCCTGGCTGCATATTCAAAATGAATTGCACAAATTCAGATAATTATTATTTAAAATTAATTTCTTATAATTTAAAAAGTTTTCAATTAATCCTTTGTAATTAGGCCTTTGCATTGGAATTAATGAAATATTTAAAATGAATTCAAGTCATTGTCTTGGTGAATATTCATTATATTTTTGCTTTATAAAAATAAAATCATCAAGCAATTTAACATTGCTCAATGTTGTTAATTTTTCATCTGCTTCAAAACTTAAACAAATCTTGTTTTTACATTTTAAATAATACGTTTTCATTACTAAACTAATTTATTTCGCCATCAAAATCGTTTTTATTATCTTTTGATTTTGGTTTTGGATCTTCCTTGTGGTCTTCCTTTGCTAGATTTTTATACTTTTCTTCAACTTCTGCAATTTCTTTTGAAAGATCACGATGCAATATTTTTTGTAAATTTTCTAGCATACTTTTTTCTCATGAACCGTCATTAGGAATATTATTTATTCCCATTCCTCTTAATTCATCGAATGCACCCTTGAAATAATCAACCATTTCGTCATCACGAACATTTCCAATTGCTTTGGCAGCTTTATACATACTATCAACTAATTTTTCCATACTTAGATCTTCATAATTCATTTCAAATGGAGAACCAGACATAAATTTATTATCAGCAATTTGTTCTTTTAATTTTTTTGCTTGTTGTTTTAAATCATCAAAACCTTCTAAGAAATTTACAAATGAATTTTTAAAATCATCCATACTGCTCATATCTATTTCTTCATCATTTGAATCGCCATCATCTGATTTTTCATCAGATTCTTCATCTTCTGATAATTCAGATTCAAGAGAAAATTCTTTAGTTCCAAACCCGCCATCATAATCTTCGATTGACATTCTACATTCAATAGGGAATGCTTCTTCTATTGCTCAAATTTTGTTTCCTAATAAATTTTCTACTGAGTTTCATGCATCAATACCATTTTCTATTTTTGTTAATTTAAAATCAAAACTAGATGGTTCGGTTTGCAATAAATTAAGTTTTTCTGATAATTCAAATAATTCGTCTCATTTGCTAAAATCACTTCTAGTTATATTTTTAATATCATTTGAAGGGTTTGTGTGAAATTTTTTAAATTCATTTTGCTTTAATTCTTTTAAATATTTTAAAGTTTCTTTTCTTTCAGAAATTACACTTTGACTAATTAATTCAGGATCAATTTGAAAATCTTCATCAAATTCTCTTTTATCATCATAAACAAAATCAATTTTTAATGCTAATGAAATAATTTCTTTTGTAGGTAAAATTGCAGAGAAAATAATTTCATTTTGTTCAAGTCCATATTTTTTAAAGTCAAAAGAATTTTTATTTTCTTCATCGTCGTTTATTAAAAAATCATTAAAAATATTTAAGGAATTAAAAGTGTTTAAATCTGACAAAAATTCTTGTGATGCTTGTAAATCACCAAATACAAATTCAAACGGAACAATTGTTTTTAAATAATTTGGAATTGTATATCAATGAAATACAACACTCTTTTCAAAGCCAAAAAATGCGCCTAAAACATCCACTAATTCAGAAAAAGAAATCCCTGAAGTTATTGGAAATGATATTGGTTCAAAATTAATTTTTTTAACTGCTTCTATTGGCTTAAATTCGTAGTCAAAAGCTACATTTAATTCTCTAACTGTTAATTTTAGATTTATTCCTAATTGCTTCTTAATCATTTTCTACTCCTCTATTGTTTTCAAATTCATGCAACATATATTTTTTAGTTATATTTTTTAATCTATATATGTAATCAAGTATTGCTTCTCCCAAACCATTTTGTTCAACAGCACTTGTATGAAAGTGGCTATGTTCATGTACAGTTTGTGTTGCATTTGCCATTGCATACGAATATTTTGATATTGACAACATTGGTATATCATTATTTCCATCCCCGATTGTCATTATTTCATCTTTAGATGTTTCTACATTCTTATAAACATTTTCTAACATTCAAAGAACAGCAGACCCTTTTGAAATATTTAAAGGTGATATTTCAATATTGCAATTCGTTGGAATCATTGCCAAATTTGTTATTGACTTAAATTTTTCAAACATTTGCAATGCTAACTCTTCCGTTTCAGTTCCATCTGCTGAATAAATTTCAATCTTAGTTGGTTCAATAAATTCACCTTTGTATAATTTAGGAATCGAGTGTAATAATTCCTTGCTTATAAAGTGATATGAGCAAATATATTCAACTGATTTTTGATTATCTTTTGTGTAATAGTAATTATTATTGTCTCATAAGAGAACTTGGAAATCATTCTTTTTAGCAATGTCAAGAATCAATTTTGTTGTATCAAAATCTATTTTTCAACAGGCAATATATTCTCTTGACTGCATATCAAAAATTTGAGCGCCAGAAGATCCTATTAAATATCTTGCTTTTAATGTTTCGGCTAGTTTCAACATTCTTTCTTGAATTGGATTTCCAGTACATATATTAAAATCTAACCCATTGTCTACTGCAAAATGGATATCGTCGATTGTTTCTTGCTTTAAATTAAAATTTTTATATATGGTACCATCAACATCTGAAAAAGCAATTGGCTTCTTGTTGTTGGTTACTTTTAAAATTGTTTGACTCATAATTCTCCTTGTTTTTTTATGCTCTAATCTTTGATTTATGTTTCTTTTTTAATACCATTATAATAATATAAATTATAATGCCAGAAATGGCAACTGGTGGCACAATATAGCTTAATAGTCTAAATGTATAATGCATTGTGGTTTCAGGTTCTTCTGATTTTAGAAATTCCGTATAATCTAATAAAAAATGATTTATGGCATATGTTTTTAAATTGAAATAATTCTTTACATTTAAATCCGAGGCATCCTTCTTTATTTCATTGCTTAATTGTTGTTGCATTTTTTTTGACACCAATTCAAACACAAAAAGTATTTTTAAAAGTTCGTCATTTGTTCTTAATCTTATTTGAAAATATTTATTAAAAACCCTATAAAGATTAGGATCAGGATCATTTTTTAATAAATTAATAAAACTATTTTGATTCTTTATATCAAAGAAGGGATATTTAAGATCATTATCCAACTCCAAAGCTCTATTGTATTCAGACTTTTCTTTAAAATTCCAATCTTTTACTCTATTGTTATAAAAATCTTCAAATCTCATTTTTTTAAATGGTTTTTTAGGGTCTTCTATATTTAGATTTTGCAAGAATAATTTTTGATTCATTTCATCAAATGAAAATCTGTGTTCTTTAAATATTTCAACTATTCTTTTGGCAACACTTAAATCTTGTTTTTCAATTCCGTCATCATTGCCTAATTCTAAATTATCTTGATTATCTTTTGATTTTTTGCACGATAATGAAACTAATGGGATAGTTAGTAAAGTCGGAATAGCCAATGAAAAACTTAAAAATTTTTTTATTTGTTTTTTATTCATAATATTTATTTATTATATTTTACTAAAATTCCAAACTTAAAAGATTTAAAAGAGAAATAAATGCTAAAAACTACACACTAGAAGTTTTTAAAAAATAACAAATTAAATCAAATTTCAAACTTTGTAATTCCTATTCTATTATTCTAGATACTTAAATCCCAAAAGTTTTCAACAAAAATTAACCTTTTAGTTCAAAAATATAGTTTTTTTTATTTTTTTCAATATATCGAATATAAACCATTTAAAAAAACAATAAAATATTCCCGGAGATTATTAAGTCTCTTTTTTATATTTACTAAATTTAAAAAAGGATAAAAAATTAGAAATGAAAAAATCAAAGAAAATATTTATAACATTATGTGGAATAGCAACTACTGCTATTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCTTTAAAACAAGCAAACACATTAGCAGAAGAATTGAAAAAAAATCCTGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCCGAAGCAACCAAATCATTTAAAGAAGCAGGTTCATACAGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAATAGAAAATGCAAAAAGTGAAAAGAAAACAATTGACGACAAGAATGCTCAAATTGCAAAAGAATTAGCAGAAAGAAATGCTAAAATTCAAAGTAATATTGAAGAACTTAAAAAAATCAATAATGAAGCTTTTGAACTTTCAAAAACTGTAAACAAAACAATTGCTGAAGTAGAAAAGAAATTTAAAATAGAAGCATCATTCAAAGAACAACTAGAAAATTTTGCAGATGATTTATTAGACAAAAGCCGTCAAATTGATGAATTCACCACAGTTACTTCAGCTCAAGAAGGTTTTACTCTAGCAGAACTAGAATCATTTAAAGAAATAACCACTACTTGATTCAATGGAATGAAATCAGAATGAGCAAGAGTTCTAGATGCTTGAAAAAAAGAAATTGAAGAAATTAACGAAGATAAGCAAATTAAAAGCGGTATTGAAGAATTAAATAAATTAAATGTTGAAGTTGAAAATCTAAAAAACGAAATAGAACAAACTATTAAAAAAGTAGAAGATAAAAAATTTAAAATAGATGAAGAATTTAAAAAACAATTAGATCTTCTTGTAGAAGATTTATCAGAAAAAAGCCGTCAAATTGATTCATTCATCACAGTTACAGCAACTCAAGAAGGTTTTAATTTATTAGAATTAGAAACATTTAAACAATTCAATACTTCATGATTCAAAGAAGTTAAAGCAGAATGAGCAAAAGTTCTAGATGCTTGAAAAGAACAATTAAAATAAGTTTTAAAAACAAAAGAATATAAATTAAAAATACAAAAATAGTGCTACTAATGGGCACTATTTTTAATTGTCAAAATTGAAAATGAATTTTTTATTAAAAGCAAAAATTTATCTAAAAGTTGTAATGTTTTTTAACAATATCAAAATCTAATTCTTCAAAGAAAATCTTGGCAGCAATGGTGGGGTTTAATTGTTGTACATTTTTTAGGTTAAATGAAATTAAATTACTATTTTTATCAATTATGCAGTCTTTTGAATAAACAAAAAATAAAGGATTTATTTTGTAACCAGATATTTCATTGTTCCCTCCCCTTTGCTTTCTAACTTCCAAATTATCCAAAGATAATGTGGCTATATTATTTATTATTGAGGCATTTTCGGTTTTCAATATTTTTTTTAAAATGCCAATTGTCCATACATCATAATGATTTCTACCGGCAGGTCTAATTTCTCCCACGATGTTCCCTCACAAAGAAATTTGATTTGCAGGAATCATTACTAAATTTCCGTTTTGATCTTCTGTACAAATTACATCAGCAAACTTTTTAGTAGGCTCTAAATAATTATTTGAAATTGCATATTCTATACTTTTTAAAGAAAAATTATTAATATTTTTACCAGCAGATTCAACAAAATTTAAGCCAATGTGTTCACTCACCATTTTAGAATATTGTTGATTTTTTTTAAGTCATTTGGGGGTCACAATCATCTTACCATCTTCACCATATTTTGGAAGATCTATTTCACTCATTTTGGCTGCTGATTCTTTAAATTCTATCGTATATATAGTTAAATCAATAACAGTTATTAAGGCACTATCAATGCCCGCTGGTCCTCCTGCCTGTATTAACAATCTAGTATTATTGTTATTAAAATAAGCATATCTTGGGACTAAATGGTATGAATTTAATAATTTAATTATATCCGCTGGAATAAAGTTTTCATTCTCCAAACAATTAACAAAATTTGTTAGTTCTAAAATATTCGCAAACGTTTGAGCAAACACACATTCACTAAATATAGCAGACTGTATTTTTTTATTGGTTTCAATACTCTTTTTTATTTGGCAATAAAGAGACGGACAATTTTTATTGATTAAATCTAAATCAAAGTTGTAATTTTTATGTTTTACTTGATTCCAAAACAAATTACGATCTGCTTTTGAAAAAATTACTTTCATTGGAGAATGTTGATAAAATTCTTCCATTATTTCTTTTTGATTTTTAGTTAAAGGTATATTTTTCATTTTTTATCTCACAATTAATTTTATTTAAATACTGAATTACTATGAATAATGTCGAAAATTTTTTCGTCTTCTTCTATTTCTAGGGTGTCAAGAAAAATCCTATTTAGTAAATCTCTGTATTTTTTGGGAAATTTTATATTTTGTTGTTCGCCAATATTATTTTTTATAATTATGTATTTATGTGATTCTTCTAACATCTTATTAGTTAATTTACTTAAAAATTCTCTGTTTTCAATTGATAATGCGTCAAAAAATATAGGCATATTATAAAGAATATTCTTAGGATAAGTAATTCCACCATCATATAATCTTCAATATCAATAAGCAAATGAAGAATTAATCATGGCATATACATATTCAAAATATTCTTTATTTGAGAATTTTAAAATAATTTTTCCATTTCTTTGTATATCTTTATTAGATGCAACTGTAAAATAGCGACAAGTATTAGGCATGCAAATAATATAATTACCTTTTTCATCTATTAACTCTTGCAACGTATGATTGTCGGATACTTTCTTTCATTTCTCAAAAATTTTTTCAAATCTGGTGTCACATTTATAAAACATTTGGTTGTTTTCATTAACAATTTGTTTTTTAGCCGATAAATTTGATTCTAAAAATTTATTATCCAAGATGATTTCACGTTCAGATTGTTTAAAACGAATCAAATGTGTAAGTTGAAAACCATGTAAAGATTTTGATTTATTAAATACAGTTATTCCGGGCCTTACAGAATTTGATTTATTGGAATTAAAGACTCCATATTTTTTGCCTCAAAAAATATTTCCGGGAACGTTATCAAATGAAACAATAAACCCATTGCCTATTTCACACATTGTTTTCCTTAATGAGTAAAATTTTTTGCCCGAAATAAAACTATATGGCGTAATAATAACAGCCGATTCAGATTGCAAAAATACTTTTTCCATAAAAGCCGAATAAAATTCTCTTGAATCCAATAAAACTGGAGTAATTTTTCAGTTTTTATTGAACTCTTGTATTTTAAAATATGGAGGATTAGAAATAACTTTACAATCTGATGGCAAAATTATGTTATTGTCAAGAAAATCACAACATATAGCATTTATTTTATCTACAATATCTCACCCGAATTTTAGCGCAATAGAAATTTTGCAAATGTTCAATGCTGTTTCATCCAGGTCATAAATATAAAGTTTGCCATCGATAATTATTTGCCTTGCTTCCTTATAGCCAATTAAATCAAGATATGACAATATCAATCTACCAGTACCGCAACCCACATCACAAATATTAGTTCCTTTAAGTTTGCAAAATCAGTTAGCCATTGTTTTAGCCACGTCGTTTGGTGTGTAATATTGGCCGGATTGTTTCTTTTGAATTTTATTTTCAACTTCTAAACCAATTTCATACATTTCGCTAAAGTTAGAAACATTTAAAAATTCATTATGTGTATTGAACAATTTAATATATTTAAAAATATAATCTCACGTTTGTTTTAAACCGTTTTTATTCACATCTAAAACATATTGATCTAAACTATATTTAGAAAATTTTTCAATATCTTGCTTTGATAAATTAAAAAAATCATCTTTATGCTTATCTACTTTTTTTGCTTCTTTTATTTCACTCATAAATTTTTTCCAACAAATAAAATGTTCTCTTTTATTTAATATAATAAATTATAAAAAAATTAATAATAAATATATTAAAAGAAAATTGAAAACTATTAACAATCAAAATTGTAAATGGTTTTAGTAATAAATTTAATAATTTTTAATTTATGCTAAATAATTGAAAAATTAACATAAAAAATTGCGATTTCTTGTTTACACTTATTTCAATCATTTCTAGTTGTTAACTAAAAACAAGATAGTTGCTAAATAAACTATCTTGTTTTTTTAAATTTATTTATATATAAAATTTAATCATTTAAGCAAAATTTATGTCATAAACTAATATATGCTTTATGTAATTCTTTTTTAATATCAATCACATTTTTAGAAATTGATTCTTTATTGCTAATATCTTGTGACATCAAATCATTTAAAGCATTTTTAGCATATTCAATGGCCTTTTTAGAAACTTCTTCTTTTTTATTATCATCATACATCATTGATTTAATAGAAGCAATTTCACTCAAATATGAAGCAAACAACGTTTTTATTAATTTATAAAAATTTTCCATCTTGATTATTTCTATTTTTAATGCATTCTCATCTTTCTTTTCTAATGCCGAATTATATTTATCTTCAATTGTTTTTAATGAATTATTCATTCATGTATGAAAATTATTAGATTCACTATCTGGCGATTCTTTAACATAAATTTGTACAAATTCTTTTAAATCATTTGTTTGCTTTTGAAGAATGGTAAATGCGATTTTTGGATCTTCAGCTATCATTAACTCTATTGATTTATTTTCTAATTCAGTTATCTCAGAATCGCTAGGAAAGCTATCTTTATCTAATATTTTTTTAAATTCTTTAATAAGCTTTCCTTGTAGAGTTGCTGGTAATTCACGATAATATATTTTACAATAATAGTTATATTGGAACAATAGTATAGAAAGTATTGAGAGATTTTTTAAATATTCTGCTACTTTCTTTCGATTTTGACTTTTAGATTTTTTATATAATTCCTTGATTTTTTCTACTTTTGTTCTAAGTTTGGTAACAAACTCTCTATTATTTTCATTAGATTCAAATATTTCATTAGATTCAAATATTTTTAAATATCGATCGATAGCATCCTCATGACCATCAATTTTACGCATTAAATCTTCAAGTTCATATTCTATGGCACGTTCTCAGAGATCCGTCTTTTTATTTAACTCTTCTATTTCACCAATTTTTTTATTTATATTAGCAATTTTTGAATCATCAGTAAGTTCGGTTGCTTCTTGTAATAATATTCTAGCATCTTCGAGATCTTTTTTAATTGTATCTAGGGTTACGTTTTTAGGTCTTTTATCAGGATCAAGAGCAGCGAAATTTTCTTCTGAATTTTGACCACCATTATTTAGCCATCTATTTAAATCATCTACTTTTCTTTTTAAATCACGTATCAGTGGTTGTTTTTCTGTTTTAATTTTTTGGTCTAATTTAGATTTTGCAACTTCAAGTGCCTTAGTTGCCTTTGTTATTTCTTCAATAGATGAATTTTCATTAATCTTGTTATTATATTCTAATAACTTAGGGGCGTCATCTGTTCCTACTTGAATTGCATCATCATCTTTAATTAAATCTTCTAAAGATTTTTTTACTTCATTGAATTTTTTCAATTTATCAGTTTTTTCTATTTTTGCTTTTTCAACTTCTTTGGTTAGTTGTGTTTCTGCATCCTTAATTTTTTGAGTTGCTTCTTTCAATGATTTTATTGAGGAATCGTTATTTAGACTTAAATTATTAATTTCTTCTAACACCTTTTTAGCCTTATATATTTCTTCAGCTGACACTTGATTACTTGAGGAGGTAATTAAAGTATTTAGTTTTTCTTTTGATTTATTAAGGCTGTTAAGTTCGGTTGCTTTTTGAGTCTTAATTTTTTGGTCTAATTTAGATCTTGCTTCATCAAGTGCTTTGGTTGCCTTTGTTATTTCTTCAATAGATGAATCTTCTTTGACATTATTGTTAGTTATTGCCGTATTTGCTTCATCTGTGCCAACTTCGTTTGCATCTTCTGTTTTAATAAGATTTTCTAATTCATTTTTATGTTCGTTAAATTCTTGAAATGTTTTTTTATGAATTTCAGCCATTTTGGCTTGTAATTCATCTAAAGCATCTTGCATTTTTTTATTAGCATTAGCAATATCTTTTTTGTTTGAAGATTCATTTATTGAACCTAGGCTATTTTTTATTTGAATTGATTTATTAATCAACTCGTCTAAGTTGAATTTTGAATAAATTTCATTATTTTTGTTTTTCTTTTCAAAATCATCTATTTTATTTTTTAATTCTTGTAATTTATTAAATTCCACTTTTTTGTCATTATTAAATTTATCCAATTTATTTTGGACTTCAGAAATTTTATTTAATAATGAAGTATTTGAACCTTCCATTGAAGCTGTATTATTTAAAGCAATTGCTTGATTAGCTTTTTCAATTTCATTGGTTAGTTCAGTTTTTGCTTGTTGAATTTCACTATCACTATCAGTTAGGTTTTTATCTAATTCTTTTGCCTTGCCAATAGTTTCACTTAATGTATTTTTTATAGCTTGATTTTGGCTATTTGCACTTTCTTTTTCGTTTTTTGCAATAGCCAATGCTTCTTGCAAGGCTTGATTTGCAGCGACAATGTCTGATTTATTTGAAGATTTAGTAATACTATTTTTAGCATCCAATGTTGCTTGCAATTTTTGAATTAATGCTGTGTAATTTGGATTGGTATTATTTTCGTTAATAAATTCATTAATTGCACTACGAGATGCTTCTAATTTATTAAATTCTGCTTCTTTTTCATTATTAAATTTATCTAATTTATTTTTAACTTCAGAAATTTTATTTGATAATGAAGTATTTGAATTTTGTATTTCTTTTGTATTGTTTGAAGTAATTGCTTGATTTGCTTTTTCAACTTCTTTTGCTAGTTCGGCTTTTGCTTGTTGAATTTCACCGTCACTATCAGTTAGGTTTTTATCTAATTCTTTCGCATTAGATAAACTTGCAGACAATTTAGCTTTTTCTTCACTAGATGTTTTATCGGCATCATTTTTAGAAGATTGAGCTGTTTGCAAAGCTTGTTTTAATTCGTTATTTGAAGCGACAATGTCTGATTTATTTGAAGATTCAGAAACTGATTCTTTTGCTTTTTTAGCAACTTCTAATTCATTAACTAAGGTTGTGTAATTTGGATTTGTCTTAACTTCGGGAGTTAAGAATTTATCAATATTTGATCTTGTTTCTTGCAATTTAGTAAATTCTAATTTTTTAGTTGCATTAAATGCTTCTAATTTTTTGGCAATGTCGTTAATTTTTGCATCAAGTGATGCTCTTGAAGAATTTATTGCCTTTGTATCATTTGAATTAACTACTTGACTAGCTTTTTGAACTTCTTTTTCTAATTCAGTTTTTGCTTGTTGAATTTCGCTATCACTATCAATTAATTTTTTAACTAATTCTTTTGCTTTGTTAATTACTTCGTTAAGCATATTTTTTTGTTGCTTTTGATCGTTAATCTTGTTGGTTAATGATTCAATTGCTTTTTCAATATCTTTGGTTTTATTTTGAATATCTCCAATTGAAGAATTTTCATCAATCTTTGTTTTAGTTAAAACTTGACTTTCATTAGAACTATCAACTTTTTTGCCATCTTCTGAATCAATTAAATCTTTTAGTTGTTTGCTCTTTTTTAAAAAAACTGCTTTGGCATTTGCTTTTTCTTGTTCCTTTTTGAATTCATTAATCTTGTTAGTCAATGATTCAATTGCTTTTTCTATTTCATTGGTTTTAGTTTCAATTGTTTTAATTGAATCACCCACTACAACATTTGTATTTTTTAAAATTGCAGTTTCATCCGCTTTTTTAGAATTATCGATTTCGTCTGATTTGATTAATTTGTTCAATTGTTGTTTCTTTTCTTCAAAAACTTCTTTTGCTTGGTTTCTAGCATTATTAATTTGACTTTGAAGTTCTTGTTTAGCTTTATTGATTTTAGAAACTGCCTGAATAATTTGTTCTATCGATGATTTATTGTTGACTGTATTATTTTGTAATGATTGTTTTGCATTTGAATTGTCAACTCTTTGGCCATCTTCTGAATTAATTAAATCTCCCAAAGCTTTTTGGGATTTTCTAAATTCTTGCATTGCTTGCACGAGTTTTTGATCTTCTTCGTGTCTTTTGTTTTTATCATTAATTTTTTTAGTTAATGATTCAATTGCTTTTTCTATTTCTTGGGTTTTATTTTGAATATCTTCAATTGAAGAATTTTCATCAATTTTAGAATTATCTAAAACTTTTGATTCATTTGATTTATCAATATCACTTGCTTTTATTAGTTCTAGTAATTTCTTTAAATTCTCTTTTGCTTGATTTATTTCTTGTATTGCTGTTTCTTTATTGTATTTTCTTTGCTTTCTAAATTTATTGTTTTTTGAAGCACATACTGATGCTATAGCAGCAGTCGAAGCAGCTGCGGCTATTAGTGAAATAGAAAGTAAAATATTTAATTTCTTTTTCATCTTTTCTCCTATTTAATTTCCCTTTTTCATATAAAAACTTAATAAATACATTTATTAAAAAATTTCCTATATTCCCTAATGGTAATATTGTTAGATAAAAAAAAAAAAAACGATATATTTTAACAGACAATAATATTCTTTTAAACCTTTTGGTTGATTTTAATGACTTTTTAATTAAAAACTGGCTTAAAATAAAAAGCAAAATTTTCACATTTTATTTTAAAAAAATAAATAAAAAAACGCCCCATATAATACAGGGCCTGCTTAAGCAAAAAAACATATTTATTTAATGATAGTTTGCTAAATAATTTGACTATTATTTAGCTTTTTTAATTATTCATTAATAAATTAATTTTAAATATTAAAAATATTAAACATAGAATTTAAAAAAATAAGATAAATAAATGATTAAAAAAATTAATAAAAAAATGGGGCGTTTGATGGGGATCGAACCCACGTATGCCGGAGCCACAACCCGGAGCGTTAAACCACTTCGCCACAAACGCCATTTTGTTAAATTGCTTATATATTTTAACATAAAATACAAATATTATTGTAATTTAGTGCCAAAAAAACTTAAACTCTTACTTTATATTTATTTTAATAACCCTTATTAATTAAACAAAAAAACAAGATAATTACTTTTGAGTAGCTATCTTGTTTTTAAATTTATTTATTTATTTATTTGTAAAATACAATGGTTTAACGTTTGAAATGATCTTTCGTTAAAGATTGAGCCGTTGCTAATTTTTCTTCAATATCTTTGATTTTTTCTTTCATTAATTTATCATTACCAAGGTCTTGATTTAAATTAATTTGTCCCAAGGCATTTATTGCTTCATCAGCATCTTTAATTCCTCTTTTAAAGACTTCTCTTTGATCATGTTTATCAGAAATTAAATATTTAAATTGTTCCCAATATGTATAAAACAATTTAATTAATTCATTTAAATTGTTTATTTTGCTTGTTTCTACTTTTAGGGCATCCAAATCTTCTTTTTGTAATGCTGAATTATATCCATCTCCAATCATTTTCAATGCATCATTAATACATGATTGAAGTTTTTTGAATCCATCAAGTTCTTCTTTAACAGATTGTGGGACTAGATTTTCTATTAATTCCTTAGTATTTTTTGTTTCCTTTTGAAGATTACTAAATGCTGTAGTAAATGGCGTAGTTGGATCTTCTTCAATAATTTTTTCTAATTTGTTTTCCAATTCAGTTATTTCAGAATCACTAGGTATTGTTTCTTTATCTAATATTTTTTCAAATTCTTTTACAAGTTTTTTTTGCAGAGACGGTGACAATTTATCGTCATTTTCATATTTTTCTTGTAATTTGACAAGCCTTAAAAGATTTACTGAATCTTCTTCTATTTTTTTAAGATCGGCTAATTTCATATTTCTCGAAAGATTATTAAAAATATTACTGTTTAATTTTTCATTAAGTTTTGAAATAAAATTATTATTAAATTTTTTTGCCTTAAATTTCTTAAGTTGCTCTATATTTGAATTAATTACTGAAACTAAACCACCTTTTATTTTAAGCATATTACTTATTCAAGATTCAAGTTTTCCAACAAATTCATCTATCTTTTGAATTTTTTGTTTTATATTAGTAATTTTTGAATTTTCAGAAAGACCTTTTGCTTCTTGCAATAATTTTTTAGCATCTTCAAGTTTATTTTTTATTTCATCTGCACTTAAGCCATCAAGCCCTTCAATAGACATAAGATCGTCTAAATTTTCAGTATTTAATAACGCTTCTAATTTTTTATTCTTTGCATTTAATTTATCCATCAATCTATCTTTTTGAACCCTAATTTCTTGATTTAATTCAAATTTTGCATCTTCAAGTGCCTTAGTTGCTTTTATAATTTCTTCAATAGATGAATTTTCATCTACTTTATTAGTTTTCAATGCTTCTTTAGCCTTAGAAGTTTCAACTTCTTTTGCATCATCGTCTTTAATTAACTCTCTTAAAGATTTTCTTGCTTCGCCGAATTCTTTTAATTTTTCAGTTTTTTCTATTTCTGCGGTTAGTTGTGTTTCTGCATCCTTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGTTGAAACATTAGTTAAGTTTAATTTATCAATTTTATCCAATGTTTTTTGAGCATCAGATATTGCATCTGCTGGTGCTTGTTTATTATTAGATTTAATTAAATTACTTAATTCTGTTTTTGCGTCAGTTAAATTATTAAATTCAGCTTCTTTTTTAGTCTTGATTTCTTGATCTAATTTAGATTTTGCATCTCCAAGTGCTTTAGTTGCGCTTGTAATTTCTTCAAGAGTTGAATTTTCATTAGCATTATTATCTTCTAAGGCTTTTTTAGCTACATCTACTCCAGTTTGAATTGCATCTTCTTCTTTAACTAAGTTTTCTAAGGCATTTTTTGCTTCGTTGAATTTTTGCAATTCTTTGGTTTTTTCTGCTTCTAATTTTTCTATAGCTTGTTTTAATGCTTCATTTGCAGCATTAATTTTTTGAGTTGCATCTTTCAATGATTTTATTGAGGAATCATTGCTTAGACTTAAACCGTTAATTTCTTCTAATACTTTTTTAGCATTAGATATTGCTTCAGCTGGTGCTTGTTTAGTAATTAAATTACTTAATTCTGTTTTTGCTTTAGTTAAATTATTAAATTCAGCTTCTTTTTTAGTCTTGATTTCTTGATCTAATTTAGATTTTGCATCTCCAAGTGCTTTAGTTGCTTTTGTAATTTCTTCAAGAGTTGAATTTTTATCAGCGCTGTTTTTAGTTAATGCTGCAGTAGCTTCGGTTGTATCAACTTCTTTTGCGTCATCAGTATTAATTAATTGTTCTAGCGCTGTTTTTGTGGTATTGTATTTTTCTAATTCTTGTTCTTTTTGTTTTGAAATTTTATTAGTTAATGATTCAAGAGCATTTTGGATTTTTACTATTTTTTCTTTTATTTGTTTAATAGAATCGCTATTAGTAATGCTAGTATTGCCAAATATGCTTAATTCATCCTTTTTATCAACATTATTTGCATCTGGTAAGGAAATTAATTTTTCTAATTCAGATTTTTTAGTTTCAAATTCGTTAGTTAATTGTTTTTTTGTATTGTCAATTAATTTTTGTAGTTCTTCTTTAGCGTGAGTTAATGCTTCAGTTGAATGTTGAATTTTTTCAATAGTTGTAGTTTTATCTACATTGTTTTCGGATAAGGTTTTTTGAACATCAGCTATGCCCACTTGAACTGCGTCTTCTTCTTTGATTAAGTTTTCTAATGATGTTTTAATATCATTATATTTATTAAATTCTTCATCCTTTTTATTTTTTATACTTGTAGTCAAAGAATTAATTGCTTCTGTAATTTTTTCTGTTTTGGTTTTTATTTCTTTTATTGGAGTATTTGAATCAATTGAATTATCTTTTAAAACATCAGATTCTTTTTTATTATCAACACTTTTGGCATCACTTAATTCTAGTAATTTCTTTAATTCATCTTTCTTGTCATTGAATGTTTTTGTTGCATTATTTTTAGCATCAATAATATTTTGTGTTAATTCAGTTTTAACACCTTCAAGAGCTTTAGTTGCTTTTGAAATAGCATCAATAGTTGAATTTTTGTCTACTACAGTATTTTTCAATGCTTCTTCAGCTTTAGAAGTTCCAACTTCTTTTGCATCTTCTTTTGCAATCAATTCTTCTAATTGTTGTTTTACTAGATTAAATTTTTGTAATTCAACAGCCTTATCTCCTTTTACTTTGGCTATTTTTTCTTTTAAGGTTGTTATTGCTTTTTCGATGTCACTTGTCTTTTGTTTAATTTGTTTAATTGTAGAATCATTATCAATTGTAGTTTTATTGTATTTTTCTTCTTCAACTTTTTTATCAACTTCAGATGTTGCAGAATCTAAAAGGCTTTTTAATTCTTGCTTCTTTGAATCAAATGTTTCTTTAGCTTGTTGTTTTGCACTTTCAATGCTACTTTTTAATTTTGTTTCTTCACTAGATATTTTTGAAATAGCATTTTTAATTTCTTCAATAGTTAATTTTTCATCAACTTCTGTTTTTAAAGCTTCGTTTGCACTATCAATGCCAACTTCTTTTGCATCTTCTTCTTTAACTAGTTTTTTTAGACTTTCTTTAGCCTTTTCAAATTCTTGAATTTTTTCGTTCTTTTTATCTTGAACTTTTTTATTTAAGGTCTTAATTGCATCTTCAATAGTTGAAGTTTTTTGTTTAATTTCTTTAATAGAATCTGTTTTAGAAATTGAAGTATTAGTTAAAACATTTAATTCATTAGTTTTATCACTAATATTATTTGCATCACTTGAGCCAATTAATGTTTTTAATTGTTCTTTTTTAGTATCAAATTCTTGTTTAGCTTTTTCTTGAGCATTTGAAATTTTTTGTTCCAATGCTACTTTTGCTTCTTGCAATGCTTTGATTGCCTTTGTTATTTCTTCAACGGCCGAATTTTCATCAGCCTTATTATCTTGCAATGCTTTTTTGGCTTCTTTTACTCCAGCTTGAATTGCATCATCTTTTGCAATCAATTCTTCTAATTGTGTTTTTATACTACTAAATTCAACTAATTTGGCTTTTTTGTCTTTTTCTTGGGCTACATTATTATTTAATTTTTCTATAGCGTCCTTTGTTTTTATAACTGCATTTTTTAGTAAATTAATTGGTGAGGAGTTATTTAATTTTTCAACTTCTGTCAATACTTTCTTGGCTTCTTCAATTGCTTTTGCTGACGCATCAGTTGATTTAGCAATTAATTCATTTAATTTGCCTTTTTCAACTGTTAAATTATTAAATTCAGTTTCTTTTTTAGCCTTGATTTCTTGATCTAATTTAGATTTTGCATCTTCAAGTGCTTTAGTTGCCTTTGTTATTTCTTCAAGAGTTGAATTTTCATTCACTTTATTATTATCAAGAGCTTTTGATGCTTCTTCTGTTCCAACTTCTTTTGCATCATCAGTTTTAATTAATTGTTCTAAAGCAACCTTAATATCATTAAATTTTTGCAACTCATTTTTTAATGCCTTAACGTTTGCAATTTTTTTGTTTAAAGATAATATTGCTTTTTTAATTGCTTCAGTCTTTTCTTTAATTTGTTTTATTGTATCTCTTTCAAGAATATAAGTGTTTGCCAAAATACCAATTTCAGTTCTTTTTTCAATTGGTTTTGCATCTTCTGAGCCTACTAAAACTTCTAGATCTTTTTTAACTTTATCAAATTCTTTTATTGAATCTTCTTTAGCACCATTTATTGCATTTTTCAATTCAACGATTGCATCTTTAAGAGTTTTAATTGCTTGGTTTATTTCGTCTATTGAAGAAGTGCTTGTTATTTTAGTATTTTTTAATGTTTCGGATGCTTTATTAGTATCGATGAATTTTGCATCTTCTGAATTGATTAAATCTTGCAATTGTTTTTGAGTTTTTTCAAATTTTTCTTTTGCATATGAAGGATTTAATTTATTTTTTTCATCCATTTTTTGGATCAATGAATCAATAGCACCTTGAACTTCTTTTGTTGCTTCCTTGATTTCTTTTATTGAATCGCTATCATCAATCTTTGCATTATTAAAGACTGTTGTTTCATTTTTCTTGTCAATATCTTTTGACCATTTTGATTCTAGCAATGTCTTTAATTCTTGTTTTTTTGCTTCAAATTCTTGTTTTTGAATATTCTTTTCATTTTTAATTGCTTCTTTAAGTTCTTCTAGAGCGTTTTCAAGATTTATTCTTCTTGTCTTGAATTCTTCAGATGATAATGTGGTATCCTTTGTCGTGTTTTCTGCTAATATCTTTTTAGCTTTTTCATTGGCACTTTTTATCATTTGAGCATCTTCAGTTTTAATTAATTGTTCTAATTTACTTTTAACAATATTATATTTGTCTAATTCACTACTTTTATTGATTTTTTCTACTTCTATGATTGCTTGTTTTTTAGCATCAATTAATAATTTAGTTGCTTGTTGAATATCGCTAATAAAAGAACTATTGATGTTTATATCGCTAACTTTTTCAATTGATTTTTGTGCCTTTGCCTTAATATCTTTGTATTTCAAATCTTTTAAATCGTTTGCAATGTAATCTGTTAATTCTTGCTTTATTTTATTAAATTCATTAAATTCTTGTTGCTTCTTAAATTTAATTGCTTCTTGCAATTCTTTTGTAGCTTTTTTAATTGTTTGAACTGCTTGAAGAACTTGAAAGGTAGCAGATGAATTTGTTATTTTAGTATTTTTTAATACTTCAGTGGCCTTGGTGGTATCTACTGATTTTCCATCTTTTGAATTAATCAATGCTTGTAATTCTTCTTTGGCGTTTGCAAAATCTAATCTTAGTTTATCAAGAGTTTTTTTATCGTTTTGATTTTGTTGTTCTTTTTGTTTTCTTTCAAGAATTTTTTGAGTTAATGAATTAATTGCTTCTTTTACTTGCTTCGTTTTATTTTTAATGTCTTGAAGTGAAGAATTTTCATCAATTTTAGCATTATCTAAAATTTTAGATTCTTCTAATTTATTAACATTTTTAGCATAATCTGATTTTAGCAATTTGTCTAAAATAGATTTGGCCTTATTTAGTTCGGCCTTAGATTTTTCAATATTTTGCTTTAATTCTTGTTGTTTTTTAAATTTTCCTTTTGCGGCAATGGCTGCTATAGTAGCAGTTGTAGCTGCTGTGGCTAACAAAGAAATTGAAAGTAAAATACCTATTTTCTTATTCATGTTATTTATTTCTCCTTATCCTATATAAACTTAATAAATAATTTATTAATTTGCTTATATTAAGTTATTTTGGAGCAAGAACCCTAAAAAAAAAAAAAAAAAAACGGTTTTCTCCATCCAAAGAGATTTTTTTGAACTTATTGGTTAATTAAATGTGATTTTTTTATTTTTTTTAAAATTATTTTTTAAACAAAATAAATAATAAATATATAGATGTTTTTTAGCATAAAAGCCTTTGGACTCCATAATAAATTAATCACAAAAATTTATAATTTAAAAACTAAATAAATCATTTTTTTCAATAATTTTAAATAATAAAAAAGGCTTGCTATTATTGCGCGCAAGCCTTGTTATTTTTTTTAATTATTATTAATATCTTGGGATTATATGAACATGAATTCTGAATACTGTTTGATCTGCTGAAAGATTATTATTTATTATTAAATTAAATCCAGTTGCACCTAATTCCTTAACTCTTTTTAAAGCAAGTTCTCTCGCCTTTAATATTAGTGATGACAATGCTTGGTCATCAATATCGTATAAATTTGTGGCGTGGTTTTTTACCGTTACTAAAAAATGGCCTTTATCTACTGGTTTAATATCTTCAAAGGCAATAAATTTTTCATCTTCATATATTATTTTTGCTGGGATTTCTCTTGTTATTATTTTTTCAAAAATATCATCATGCATATTATACCAGACCTTCAACTTTCAATATATCATAAACGGATTTATTTGTTTCTGAAACATTTAATTTAAACCCTAGTTCATTAATATATTTAACTGCAGTTTTATATAATGACTCAAAATTAGCAGTCACTATATTATTTGCAATAATTTCTTGTTTTTCTTTTGTTGCTCATGGAGTTTCATCAAATGTTAAAACTTGTTTTGTAACTTCTTTGCCGTTATAATCTTTAACTTTCTTAGTTAAATATAATGGTGAAATTTTAACTACATATTGGGCAGTAATTGAATTGTCTTTTGTAACATTTATTTTTTGAGGATTTTGTTTTAAATTGTATGCAACATCTTGTTTTATTGACTCAAATTTCTTTGTATTTTTATTGTATTCTAGTTTAGCATTGAATTCATAGAAGTTTCTTTTTCCTAAACCATCAACTGTTAAATTCATAGGAATTGTTAAAAAGTTTCCGTCAAATGATATTGAACCTTTTTGGCTAAATCTCAATGTATATTGCTTGTTATCAAGAGTTATTGTCTTTGTATATTGATAATTATCCTTGCTATCTTTTGTTAAACCTTCAAATTCGTAATCTTTGTAATCAAATGATTTTAATTCGCCCTTTGAAGCTTTAGATTGCAATGTTTCGGTTGCTTTTGCCTTTGGATGGTTTATTTCTTTGGCAAGAATTTTTGCAAGTAAAAAGCTTTCTTGACTATAAACTTTTTTGCTATATGGATCTACAAATCCATATACTGAACTTAAATCTTTTTTTATACTATCTAAATTGCCACCTAAATCGCCTGACGTATTTGAATTTTTAACAATGCCTTTATATGCCAATAATGATTTTAATACTTCGCCTTCCATTGAACCGCTATTAATCAATTGTTCTGGGTTTTTAGCTGCTTCATTATGACCATATTCTGGTTTAGTTGAAGGATTGTAAGAAGCTAGCTTATTAAATCCTTCAATTGAATTTATATATGATTTTGGTCATCTTAAATTCATATCGCGCTTGTCGTTAAATTCTCAATTTTCAATAATTGGATTTTCAACTAGATATTTCATTAAATACAATGAATCATCTGCAAAATTCAATGCTTCGTATAATTCTTTTTCATTAATAGGAGCATCTTTCTTAGACATTTTGTTGTGTAATGCAACTTGTGCTTTGTCTAGACCGTTGCTATCATTTGCTATTTTTTTAAGTTCTGGTCTATTTTTTAATAAATAAAGTTTAGCAAGCAACATTTTTTGAAGTTCTAATCTAATGTTTGCCGCGATTTGATTTGAGTGTTTAAAAATAAATTTAAATTCTTGTTCACTTGGTATTGAAAATGCAGCAGGATTATATTTAGTTGTATCAATCTTTGCATTGATAAACAATGATTTTAAATTTCAAAAGAATTGAGGATCGGAAGCAACTCTATTAGTTGCATAATATTTGAATAATTCATAAAGATCTTTTGTTAATGCTGAATTGCTAAAATTTGAAACAATATATTCAATTTTGTCTTTATCTTTAGAATTTTTAGCATAGCTTTCAATTTCACTTTTATAAAATTCTGAAATATATATATCAGTAAGTCAAGTTAAAACCCTATTTTTTGTTAATGAATCTTTTAAAACACGTTTTTGGTACCCTGAATTTTGTTGAGAATCTTCCTTTTCTTTTTTACATGAACTAGCAATCAAAGGAAGAACCGCAAGTGGCATTGCAATAGCTGTTAATAATCTAATTTTTTTAGTCATTGTATCTCCTTTTAATTTTTAGTATTTATGTAATTTACAATTGTTGTGAATAAATAAGATAAGATCTCTTTGTCTTCGTGGGGCGATAGAATAAACTTATTGTCTTTGAACACATAATCTTTGTATAAATTACTATTAAATTTTTTAATTATAAATTGTTTTATTTCGTTTGCTTTGCCATTAATTAAAACGTATTTGCTTGTTTCACGAATTAATTTTTCATAGTTGATTGCATTTAAAATATCTTGATCATTGAATTTACGTTTTGTTCCTTCAAGACTTAAATATTCTTTTGATTTAATATAATTTTTAAAATTATCTTGTTGTAATAAATCATTAACCATATAGTCACGAGTTAAAATTTCAGAAAATATTGAATCTAAATTAAATAATTTAGTATTCAATGTATCCGAATGATTAGCCTTAGATAGTATTGACAAATCGTTTGTTATCAATCTAGTTACAACTTCTTCTTTGTCTAATGAAAATACATTTTGAATCAAAATTCCACTTTCATTGACTTGAATGTAATTTCCATTACTTTTAATTAATGTTGAAACCTTATATCCACTTTTTTCATCACTAAATGCATCTCGAAATGCCAAGCCAAAAGATTTATTAAAATCTTTTTCACTTAAATCGTTGATAAATTTTTCAATAGCTGCATTGTATCTAGTGTATTTTTCAGAGAAGTTTTCTTTGTCAACACTTTCAGTTCCTTCATTAAAAATATCAGCAAATTTCTTTGTTTTCTTTTCATGATCAATAAATGTAGCATCGCCAAAGATCTTTTTGAAGTTTTCTTTTAATTTTTTAAACAAATCATTTTTATCTTCAACTTTAAAAATGCTTTTATCTTTGTCAGCATCTCCCATTAAGATTGAAAGAAGATTCATATACTTATTAGGATCTTTTTCTTTTAAACTATTCAATACACTTTTAAAGCCGTCGAAACCGTATTTATCAGCTGACTTGCTATCGCTTGAAACATATTTCAAAAGGTTGCTATCATTTTTTGCCGCAATTTCTTGTTTTTTAGTAATTTCATTTGTTTCAAGAAGTGAAGATATACCTTTAAATGCTGAAATGGCATCAATGCCTTGTTTAATTGAAACCTTGTCATCACTTTCATTCAAATAAGCAGAAAAACTTAATAATTTAATAATTTCTGCTTTTGTTACGACTCATGGATTTGAGGCATCTTTTGGATCTGGATGTGCCAATAAATTTAAATTAGATGTTATTGCTTGCTTTCTTTCAATTCAATTTTTAATGAATTTTAAAGGATTGCGATATTCTTTAACAAATGATTTTGTAACAAACATCGGAATTTTAACTTCATCTTTTGTTCCAATTTTTAATGACTTATCATCAGATTTTGGCAAGACATAATTTTTGTTTTCTATAGCAAAAGGAAGAGTAATTTTTTCACCCTTCTTAGCAATGTCAACTCTTTTGCCTTGATATGTGTAATAAACATCTTTGTTTGCAATAATTCCATTTTTTAATTCACTATAAGTAAAATCACTATTTACTTCGGTTGTATATCTATTAAATGCATATCTATTTAATTTTTGTGCTAGAAGGTATTCAATTGCTTCTTCTTTTGACTTTGCTTTTCCATATCTATCAGTTGCAAGTTCTTGTAAGAATTTTTCTTCTCAATTTTTTTGTAGGCCAAATTGTTCTTGATATTTCTTTTCAAGATCTTGAATTTCTTTTGTTATTTCTTTTTTAACTTCTTCGATTGGTTTTAGTGCTAATTTTTTAATATCTTTTTCTGCCTTATCAGCATTATAAACAGCTTCATATCACAATGAACCTTCATATTCTTTTTCATAAAGGTATTTAATTAATTGCTTTTCAACACCTTCAGCAGCATGTTTTGATGAATTTGCTATTTCATGGATTTTATTAATATCACCATAGTTCAAATTTAACCTTTGACCATCTGGCCCGGTTATTGATAAAATTGAAGAAGAATCTTTTAATAAAGGTGAAGGCTTAGGTAAGGAATGATTAGCTTGTGCTAACGGAACGCCAATTCCTGCAGCAACTGCGGTTGCTATTGCTGTGCCTCAAAAAGCACCTCAAATAATTTTTTTACGCTTAGCCTTTTGTTCGGTTGTTTTGGGTTGATTTTGATTTGACATAGTAAACCTTTCTATTATTTAATATTAAATAATTATACTATTTTTGGTTATTTTTCTTTATTTATGGATATTTAACCAGTATTATTTTATAATTTAAAACATGAATAAATTACTTTTAAATAAGGATAGAATATTGCTTGAATTTATTGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTGAGCTTGTATGGCGCTATGTCAAAAGAACATTCTTGGAATAATTTAGATCACCATGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTGCATTCTTTGTATCACGACTTACAAATAAAATTTGTTGATGATAACAAAAATATATTTGAAAACCAACCGTTTTTGAATATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATTGCAATCAAGCTAGATCGTTTATTGGCCACTATTTAACAATGACAAATAGCAACGATTGAAAAACAAAAAATAATATTATTAAATCTCATTTTCTATCAATCTTTTATAAGCCAATTACATACAAAGAAGTTGCTAATTTATTAATAAAAAATGATCACCAAGGTTTTTTGATCTCAAGTCCGATAATTAAAAAGGATTCATTGAAATATTGATTGCCAAATATTAAATTTGAAACAAAAAAAGTAATTATAAACAATATTGAAGTTGATGTTTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATTCCGTTCCACAAAGAAAGATAGAATATATTAACCCCATTGAATATAAAAAAATATTTAGCTAATTTTTATAAAAGAGAGTTCACAATTGCTTTTTATTGAAGAAATTTTAGAATTTCTAATGTGTATTGAAATGTCTTTGTTTTTTAAAAAATAAGCAAATAAAAAAGTAGGAAGTTTCTACTTCTTACTTTTTTAAAATTGACTTTCTCTATCGATTAATCATTTTCTTTCTTGAAGTTTTACTTCTTTAATTAATCCCAACTCCAAGTATATAACTTTATCAGCAATTTGGGCAACGTCAGGGTCGTGGGAAACAATGATAATGGTTGTGCCATATTTTAAATTGATTTGTTGCAAATTTTTCAATACAATAATTCCTGTTTTTTCGTCCAATGCTCCTGTTGGTTCATCAGCTACAAGAATTTCGGAATTTTTTGCAATGGCTCTTAAAATTGACACTCTTTGTTGTTGACCACCAGACATTTGAGAAGGATATTTATACATACATTCAGAAAGATCAAAATCCTCAAATAATTTTTTAATATTTAAACGCTTGTTCTTATCTCTTTGCAAATATGAACCAGTCTCAACATTGTCATAACTATTCAAATTTTGAAGTAAATTATAATTTTGAAAAATGAACGAAATGTTTTCTCTTCTAAATAAAGTTTGCTTTGCATCAGATAAATAAGGTAAATTTATGTTGTTTACAATTACATTGCCAGAGGTAGGCCGGTCAAGAGCACTAATTATGTTTAATAATGTTGATTTACCTGACCCAGACTTTCCATATAGAACAACAATTTCTCCTTTTTTAATTGAAAAAGTTATGCCTTTTAAAACTTCAGTTGCGACATTGCCAGATAAATACGTTTTTTTAATATTATCAACTTCAACAATGTTATCGTTAGAATTTTGCAATTTAGCATACTCGTCTTTTTTTCTTGGTTTGTCATTAGCAAGTCTATATTTCTTTAAAGTTGCATTATCTAAAACCTTAATTGCAATGCCTTCATCTTTAATTTCAAATGGCTTTTTTTGCTTAGAAATATTTATCAAGTTCAAATCTTGCAATGTTTGTTCATCAACAAAATTTTTATTCTTAGAAATTTTATTTGTTTTAGCTTTAATTTCTTTTTTGTTTTCTTTTATTTCTTTTGCCATTTTATTTTCCCTTCAAAGCATCTACTGCTTTTATTTTATTTAAACTATTTCATGTTGCAATTGATGTTGCTATAAATACTGCCAAGCAAATTAATGCACTTAATATTACTGTTGAAATATGTAAAACCAAAGGTAATACAATCTGGCTAGTTACCATCATAAACGAGTTAAATATTGCTATTATTGCAAGAGTTGCAGGAATCATTAGTAAGCAAGCAAATACAATTGCAGGTACAAAATTAAAGAAGAATAATAGAATTTTTTCTTTATTTGTATATCCTAATATAGAAAATGTAGCAATTGATCTTCCATTTGAAGCAATCATTATATTAGTAATAACAATTAATATAACTATTGAAATTATCAATGAAATTATTATAAATGCCAGCAATATGGTATTAACTGTTGAAGAAATTCCTTCAATAAATCCAGCTTCAATGTCTTTACTATTAACATCAAATGATGCACCATACATTATATTCTTGCCATATATTGAAGATGCTTGTGTGCCATAGAATTTAGTTAATGTTTCTCTTACTAAGATTTTATAACTTTCTAATGGGCTGTCTGAGTCTATCATTTCTTGTATGCTTTCATCGTTTAGATTTCCAAGTAATTTCTTTAATTGAGTTTTATAATCTAATTTTGCCTGAGGATGTGCTTCATTATATGATTGAATGTTATGTTCATATACAGAAATATATTTCAATTTTGGATTGCTTATGAACATGCGTTTAAAGAAATCTCATGCGCTATTGTCATTTGCTTCGGCAACATCATATGAACTTGCTGCACCTCAGAATCCTGTTGCTGAATAAGTTGTTAATGTATCAATAGTTTGAACTGGAACTTTATCAATTGATAAAATACCATTAAATGCATCATATTTTTTATTGAATTTTTTAATATATTCATCTTTTAAATCAGGGTGTAAACTAATTGCTTTTTGTAATTCGTATTTTCTTGCATCTTTTAATCTTTTTGAAAGAGTATCATAGCCTAAAATGTGGTCTAAAATATCCTTCCTTGTACTAATTTCAGAGTTAATATAAGTATCTGATATGCCAATTACTTTAAACTTATAATCAACTCTTGGACTTTTTTGCATGAAGGCTTGATATGCAAATCTATCAACGTGATTTAATATTTTGGCATCAAATACGGAATTAACTTTTAATCTATAGCTTCTTGCTGTTACTTCATTTACAACAACAGGAATTGGGTCATTACTATCAAAATTGTAATTATAGTCTTGTAATAATTTTGTTAAATTATTGCCCCGACTATCTTGCAATCTCATGAATTTACTATCATTATAATATCCATAAATTTTAGTTTCTTTTCCATTTAAAGTTACTGAAGCATATGTGTATTTTTCGTTAGTTGTATCGTTTCAATAAACTCCCCCAAATGAAACATAGAAATCTATACCATTTATTTTTCTATAAGCATTTACTAAGAAATTACGATATTCTTGTCTGAATTTTGCTGTACTTACTTTGGCGGGTTTTAAATAAATTTCATCTTCTGCAACTCATTCAACAAATCTAAATTGTTTTCTATTTCCTGATGCTGCTTCTGAATAATTACCAGCGCTAAACAAGAAGTAGCTTGTTCTATTTGATAAATCTTCAGGCCGTTTAGCATTATAGTCATCAAAGAATTTTTTAATATCTTTTACAGCGATTATTTTTGTTGGATCTTTTACATCTTCTTCATCTATTATTCTATAATCTCCACCATTTTTCAATCTACGTAAATTTATTAAGTTTTGAGTTGATTGCATCATTGAACTTACGCGGTTAAATATTTGGTTTACTCTTGCCCTTTGTGTTTCAGGCATGTTGGCATAAGTTATATCTCAAGGACTTAATTCAACTGATAAATCCATTAATAAATCAAGCGATGATTTTGTAATAACAGTAGGCGCAAATTTTCTTTGAATTACATCAGTATTAAATGAAGCTCCAGGTCTTAAGAAATTAGGATTATCATAATCTAGTTGACTACCATTGGTTGATGAATCACCTGCAAGATCATTAGGAACATACAATAAATCATTTATATCGTTTTGATTGTATGTAACATAAGGACCACCTTCTGTTGTTGGACTTTCAAGATTTAATTTATATTTATATAGCCTATTTTTATATGTCTTTGAAATAGTTTTATTAAACACGTTATTTGAAGATAAGAAGAACATAGAAATTAATGAAGTCGTTGAAAATGCTAAGAATAACGAGAACATTTTTCAGAAATTATTCAATGCCATCGAGGCAATAAATCTTGCTTTAACTGGCATTTTTCTAAACATAGCAGAAACTCTTTGGGCAAAATTACCAACACTAACTTCAGTTAACCCACTCATTAATTCTGTAGGTTTATTTCTCACAATAACTCTAGTAATTATATAAATAACTAGTGAAACAACAATCAATGGAATAATAACTACTGCTAATATTAATAATATATTAAATGGAATAATATTGTTCTCTAACGTTCAATAAGAAGATAATATTGACATTGCAGGTTTTTGTAAGGCAAATCCCACAATATAACCAAATATTCCGCCAACAATAGTTGGAATTCAACCAAAAGCACAGAATGATAAAGCGATTTCTGATGTTTTATATCCTTGAGCTCTTAATATTCCAACAACCTTATTTCTTGCTTCAATATATCTTTTGATAATAAAGTAAACAATCAAAATTACTAATATTGCCAAAATTGTTATTAAATAAAGTGTTGCATTTTTAATTGATGTTACTAATGTTCTTATTGTTTTTACTCTGATGTAACGTTCAGGGTTGATTGCATCTGGTTCATCTTTTAAAAATACTTTATTTTTTGAATTTGGAGAAATTGATGAAACAATTCTTTGAAGTTCTTTCTTTAAGTTTTTAGGATCTTTGCCAGCTAAATAATTTCCTTTTTTGTCAACAGGGGATTTTACTAATGCATAACTCTTTAAGGCAAATGTAGGATATGCCGACCTAATTCTGTCAAAACCTCTTGAATTAACATAAACAACAGATTGAGTTTTTGTATCAACTTGTAAGTTTTCTTCATTAATTACAGGATATAAATAATCAGCGGTACTATCAATACCTGTTATCAAATATTCTTGAGTATTTACTTTAATCTTATATTTATCATCTAATGAAGCAATGAATTGTTGCATTTCAAATGGATTATTTAGATATTTTGAAATATCGCCATTATATACTTCTTTTTTATTTTTGTTTAAATATCCATAATTTACCTTGGCTAAATATGAAGCAGCTTCGGAATATGCAATTAAATTATAAACTGTGCTTGGTTCTATTATTTTTGAAAATACTTCCTTAACAGTTTTGGTAATATTTATTCCAAAAAAGCTAATATCTTTGTTATCGGGTGTTTTTAATAAATGGTTAAACATATAATTGAATTTAGAAATATCTAAATCAATTCTGCTATCGCTTTTTGAAGTAACAACATTTTCAACAAAATTGAATTTTTGAGTTGCACCTTCGATTTGTTTTTTCAACATTGTTGCATTAGTTTCATCAATATTTGATAAGAATTTTTTGATTATATAAGCAATATTTCCACTATCTGGGTTTGGACTTAATACGTAATATAACAATATTTGTGGGAAACTTAAAATATTGAAACCATTTAGAATATTTCTTGGTACGTTTAACAACGAAATTATTGAGGCGGTTTTTGAAGCTGCAATTGCATCCTTATATACTAAGCTAAAAGTATATTCATCATTTATTAACGGTTTAATTTTTTGGTTTTGATCATTGACATTAATAAATGATTGTAAAAATAATTTTAAGGCTTGAAGATAATGAGTTGGCATTTGATTTGAAGAGGCCATATAACCAAAATTGTAGGTTATTTCATTCATATTATTTTTTAATATCTCTTTAATTGCTTCAATGCCCTTATTAGAAACATGATTCTTAGATAATTGGTCAAATAAATTTTTAAATACTGCTGAATTATTCATTAAATCCATAATTTGTTTTGTTGCTTTTGCTGTATGTGCGTGAGCAAAAGGCATTTTAAATAATTTAGCAGAATAGTCATCGTCCGAGCCTAAAATTCCAAGACTAGGAATTGGGTTAATTACTTTTTTGTAATTTAATACTATATCTTTAATTTCCTTTGATATTCCTATGCTGTAAAGACCTAACAATATCTCTTTAATTTGCTTGTAATCTAAATATTTTGTAACTTTTCGTTCAATTATTCTCTTATTAGTTGTTGGATCAATAACAATATTTTCTTGAATATCTGCAATTTGGCTTAGTTGATATGCAAGTTTAATTCATAATGAATATAGAGATAAGACATTAGAAGCAACATTTTGTGGATCATTATTCAACATTAATTTTGCTAAATTGTCTTTTATTAATGAATGAATAGTTTTTAAAATTTCCAAATCTTTTGCATGTTCAGGCGCTATATTATTAAATGAAGCTGAATAATATGCAACATCCAAATAAGATTTTAAATCATTTAATTCAACTGCCGAATTTATACCGCTTTGCTTATATTGAGAGTTTGATGTTACAAGTTTGTCTATATATTCATGATATTTAGTTAATTTTGAAATTATTAAATCAAGGTCATTAAATTCGTCTTTGGTAACTAAAACATTGTTTTTTAATACATCAAATTCGTTTTGAGTTAATTTCAACTTTTTGTTATTTATTTTTGCATCTTTTGCCTTTAATAATATTTTTTTAATATCATCAACATCAAAAGGATTTATTGGAATATTAGTCAATACATTTGTTTGCTTTTGAGATTGAGGGAATGAGAATTTTGACATGTTTATTAAATCAAACAAAGTAATTTTATTATTGTCATAGTCAGGAATAGTTATATTCAATTTACCCAAAATTCTCTTTGTCTTATTTGAAAAATTAAACATTTGAATAACGGATTCTTGAATTTGGGAAATTTTTCCGCTATTTATTTTTCCATCTATGTTTGAACTTATTGCACCAAAAAATGATGTTAAATAATCTGAGCTAGTTAAACTTTCTGTGTTGAAATCTTTATAAATTTTATTATTTGCAACAATTGGATAATGTTCATGTTTAACTAGTTGCTCCAAGATTTGAATAAATCTATCAAGATCAAAATTAGCTATTATATTTTTTAATCCTTGTTTAAAATTATCATATTCTTGGCCGGCTTTTGTTGCACCGTTTAATTTTCTAAAAAATTCTTTAATTCCATCTTTTGTTTGTTGGTCTATAAAATTGTTAGCTTCTCTGTTTGCATTAATTCATTTTGAATACAAACTATTAGGCGATTCTGGATTTAAAAAGCCATTAAAGTCAATATTATCAATCAATTCAGACAATGTTGATTTTAATTTGTAAACATCAATAGATTTTAAGAAACTTATTAAAATTCTTTCCGATGATAGTATTCAATATGTATCATCAACACTAGTAAAAGGCTTATATGGATGTTTTTCAAATCAATCTTGAATAATTAAAGAAAATTTTTCAAAATCAATAGAGCGAATAACATTTCTCAATACTTCAAAAATTGTTGATTTATTTTTAACTAAATTAATTATGTCAAATAAACTTAATTTCAAAGAAGGAATCAAATATAGATTATTAAGATTTAATAGTTTGCTTATGTTGTAAACTTGTTGCATTAAATATTGATTTCGTTCATTGTCATTGGTTCCTGAATTCATGTACATTTTTTCTAAATAGTTTATTATTCCAATGAATACATTTTGAACAAAAGCATTAACATTAACATTATTAATATTTAAATTGTTACTATTACTTCTATCTACTGGTCTAATAATGTATTTTAAAATATCTAAAAGAACTTTTTGTAAAATCGTGCCATTTACCTTACCAATATTTAGCAATGTATGGAAATCATTATCCACAACACTTTGTTTTGCTGCATCCACGATTCTCAATAAATCGTCTTTATTGAACATGTTAATAATTTCACTATTAAATAAGGCGTCTCTAATAACATTAACTAAACCATTTATTGTTTTATTTTGAATAATTTCGTTAGTTAAATTGTTGTTTATTGCCCCGAAACTTATAGGCAAACTAAATGAGTTTTTAAACTCCTTATCAGCAACTGCTCAACCGTTTGGGCCGATTTCTCCTCTAATACTATAATTATTTCTAATCATGTATTCATAAAGTTCTTGAGATGTTAAATATGCCTTTCCATTAACATCAACTCTATTTCAAACTTTTTTATCACTAAATCCGTCAATAGTTGAATATTTTAATAAAATGTATTTTTCATCACTAATTCTTGCAACTGCACCAACTATTGAAGAGCCAACGGTCTTGGGTTCATCATTAGCAACAGTCAACATCAATATTTTTCCATCGATTTCATAAGGTATTTTTGTGTTTGGCAAGAAATCATAATATTTTAGAAATCTGATATCTGCATTGAAATAATTAATAGACGGCGTTCATTGCTCAAAAATATATTTAATTATTTGTTTTGTATAAACTGGTGGAATTTTTTTGATCATTGGATCGTTTGGATCGGGTTTCAAAATAAATTCATTAACATTTTTATCTGATACAGAAGAATTTAGTAATGTAGGATTTAGAGTTTCGTTATATAATTTACCAACGTTATTTTTTACGCCATAAATTTTTTGGTTTCCATCGCCTGCATTAACAAAGTGAAAAACGTTTTTCTTAGTAGTTTTTTCATCAACGGATTCCACAGTTAGAGTTTGTCTAATTCCTAAGTTTTCTTCTCCAACATTATTTTTTATTTCATTAACTAAGGCATTTCTAGCATATGAAACAGACCCTTTTCTAATAAATTCTTGATATTTTTTTAGATTATTAGGATCTGATATTGCTTTAAATTGGTCTTCTGTAATTAATTTATCACCGGATTTAGAATACTCAATTTGTTGAATGTTTCAACGGTGTTCTTTAGAAATGTTTTCTTTTATAGCAGGAATTTTGGAATTATTAAATCCACCATTTCAACTGTTTTTAAAAGTTTGCAATAAATCTTTTTGTTCAATTGGCAATGTTGTAGTTTTTTCTTGTTTCAAAGAAGACGAATTTAATTTATAAATTGTTTTAATCGTCTTATTTCAATATGAAAAATATTGAATATTTTTATAAACATTTGGGTCACTTTCTTTTAATTTGGCAATGTACTTGAAATATGATCCTTTTCAATTTGAGCTATATTCGTCACTCTTTTCGTTTCAATTTAATGCAAATCTATATCAATTATATGTTGTTAAAACCTCGGTAGATTTTATTCATTCATTTGGAATTTGATATATTTTATTTGCTGCTAGTTTTGTAAATTCATTAACATCTTTTTCAATACTTTTATTGCTTAAAAAATTTTGAAGTTTAAAAGTTCTTGAAATATTAAAGTCTTCATTAATAAATTTTGATTTTTGACTTGTAAAATCCGAATTTTCGATTTTTAAATCAGAGTTTTTATTATAAAGAGATGCATCAAATTCATAATGGTTTTCTGAAATTTCTTTAAAACCAAGCATCTTCATTACATCTTTACCATCAATAGTGTGCAATGAACCACTATTTTTTCACGCTTCATAATCTTTAGTATCAAATGATGCTTGTTTAGTTTTAACATTTAGAAATAATGGAGAGGGATTGTATAAATAATCAACTACTTTTTGATCGTTTAGTGAACCTCTTGGACCATATGCAATAGAAGCTATATTTTTTAATCTAACTTCATTTAAAAAAGTAATGTCATCATTTTTTGTCAAATTATATCTTTTAGTTAACGGTTTTATATTTCCTTTGCTATCTTTTATATAAAGTTTAAATTCCCTATCGTGACCTGAAAAACTAAAATTCCTAATTTTAGAAAAATCAGGGTTGGATTTTAAAATTTCAACGTCTACATCACTTATAGATTCCTTGCTAGAATATAATAAATTTAATAAATCATAAGTATTTATAAAATGGTTTTCATTATCATAATTTAAATTATTAAAAGTATTTTTTAAACCTACATAATTTCTTTGATCTTTTGGTAGATCTATTGAATATGAAATATCACTTCCATTATTAGAAGACTCAAAAACAACAGGTTTATCAACAATTGATTGATTATCAGATCCTATTTGGTTATAACCTGATTTTGGTATCACACCTGATGGGTTAACGTCAAAATCAACAGTTGCATCATGCAATTTTGAAACTTTTTCATACGAATTAATCGTAGATGAATAGCTAGTAGTAACATCATTTAACAAAGTAAATAAAAATGTTGTTAAGAAAATTAAAATTGTTAAACAAATCAAGGTAACTTTATTTCTTGCCAATGATTTGAAAACCTCTTTAAAAAGTCTTCTCATATTTCTTCTCCAGTTTAAAAAATATAATTTTTTAATATATTAAAAATATAAAAATATGTGTATATTATAAATGTTTAAATCTGAAATAACAAAAAAACAGCACTTTTAGACCAAAAAATGGCTTAAAAATGATGTTTTTTGTTAATTATTAGTTATTTTTTAGATTATTTTTGATAATTAAAGATCATTTTCTAGGAAATCCTGAGGCTGTTTTTATTAATTTTTGGTGAAAAATTAAAACATTTTCAATATTGAAATATATTGGCAAAATCCTTGTTTTTAAATTAATATTTAATTTGTTAACTATAAAGTTTGAATTAGCTATTTCTTGCTGATATTTATTTGATTTTAAAAAGCAAGCAATTCCATTAATTTTTAAAAGATGGTGTGAAATTTCTAATAAATTTTTTAATTCACTCACCGCTCTTGCACTAATAAAATCAAAACATTCTATTTGATTTGAATCTTCAGCACGAATTTTTTGTATATCAATATTTTTCAATTTCAACTCATTTTTAACCAAATTTAAAAAATTTACTCTTTTTGATTGGGGTTCATAAATTGTTAAAGAAAAATCTTGTTTATATATGAAATATGGAAGGATAGGAAGCCTGCGCCCGAGCCAATGTCGAGTACAGATTTGTTAGTTAGATTACAAATATTTTTTTCAATGTCTAAAAAAATTAAAATTGATTCAATTATTGCTTCTTTTAAAAGCTTTTCGTTATAGAAACCTGTTAAATTATATTTGCTATTTTCTTGTTCTATTAATTGATAATATTCATATAATTTTTTTAAGACTTCTTTGCTACATTGTGGTAGACTTTGCAATATTAAATTATTACTTTCTTTTTCCGTAAGCATTATATACTTCTGTTATAGATTTGCTTTCAATGTTTGCATTGATTACCATACTTAAAAATTGAGCCAAAGATAATTCTCTTAATTTTTTAAAGTTTGATAAATGGCTGTGATCTATTGAATTAGTAATAATAACTTCATCAATCATTGCATTATTTTCAAACATTTCAAATCCTTTTGAAAATAAACCATGAGTGGCTGCAATTATAATATTTTTTGCGCCGTTTGCTTTTAACGCTTCTGCTGCATGAATAATTGTTCCACCTGTGTCAATAATATCATCATAAATTAAAACATTTTTATCTTTAACATCACCTAAAACACCCATAATTTCGCATTTATTAGGTCCTGTACGACGCTTGTCAATAATTGCAATTTGTTCTTCATTGTTTAGTAATTCTGACAATTGTCTTGCTCTAACTGCACCCCCGTGGTCTGGTGATACAATTACTAGTTTTTCTTTTCTGTTTAGCAATTCATTTGAAAAAATAAATTGTCCCCTTAAATCATCAACAGGAATGTTGAAAAATCCTTGAATTGAAGCGTTGTGCAAGTCAATAGTAATTATTTTATCAGCACCCGCTGTTTCATACATCTTAGCAACTAGTGCAGCTGATATTGGATCTCTTCCACTTGCTTTTCTATCTTGTCTTGCATATCCATAGTAGGTCATAACGCAATTAATGCTCTTTGCTGATGAACGTCTTAATGAATCGATTAAAATTAGCAATGACATTATGTTATCGTTTACTGGTTTTGAGGTATTGCAAATAATGTAAACATCCTTATTTCTAACAGTTGTTTCTGATTTTAAAAGGCATTCTCCATCTGCAAATACTGTAGTTTTAACTGGCAATATTTCTTTATTTAAATACTTTCCAATTTCTTGCGCTAATTTTTGGGAATTTTCCATGCCAAAAATTACAACATTATCTTCTTTTTTTGCTTTCATTTAAATCCTTATATATATTCTCTTTAATTATTTATAGTTAAATTTTACATTATTTTATTTAAAAATAAAATATTAATAAATTGCAATTAAAAAAGTGCAAGTTGCACTTTTATAAAAATTCTTTATATATACGTTTATATATTGACTTAACACTGACAACTAATAAGACATATATTCCTAAAAATAAAACTAATCAAGCATAATAAATTCCTGGCAATGGCATTAGACTTAAACCTTTATTAATTCCAGGTATATATGGCACGGCATTAATTATTGCACATCCGATTAATGTTGAAATAAATAACGAGGTCGTTGCATTTGATTTAATAAATGGTGTTTTATCAGTACGCAAAAAGTGAATTACAAGGGTTTGAGTATACATAGAAACAACTAATCATCCTGCTCAGAATAGTTCTTTAAATGCTTTTTGTTTTTCTATACTTAGTTCATCTTTTAAAGAGGCAAAACTCTTATATGCTCCTATTTGTACTCAATCACCCTTACTAGTCATAAATGACGCATTTGGAATTATTGCAAAGAATAAGACTACATATGAAACAATATCTACTATTGAACTCGTTGGACCAAACCAAAACATAAATCTTCAAATTGATTTATACTCTCATTGCCGAGGTTTAAGAATAAATTCAGGATCTACATTGTCTCATGGCATTGTTGCACAGCAAACATCATATACCAAATTCAATAATATTATTTGTACAGGGTTTAATGGAACAAAAGGAATTAAAAATGAAGCAATTAAAATACTAAATATATTTCCAAAGTTTGAAGATGCAGTCATTTTTATGTATTTGTTAATATTGGCATGTGTTTTTCTACCTTCAACAATTCCTTTAGCCAAAACGTTTAGGTCTTTTTCTAAAAGAATTATGTTAGCTGATTCTTTTGCAATATCAACTGCAGTATCAACTGATATTGAAACATCGGCTGATTTCATAGCGGGTGCATCATTAATTCCGTCACCCATATAGCCAACAACATGACCATTATCACGCAATATTGAAATAATTCTTGCCTTTTGGTCAGGGCTTAATTTTGCAAAAATTTGAACTTTTTCAACTTCAATTTTAAGTTCAGCATCGCTTAATTCTTCAACTTCTTTGCCTAGTAAAATTCTATTATTTTTTATTCCTACTTGGGCACATACTGCTTTTGTTACAAGTGCACTATCTCCCGTTAATATTTTTACATCAACTCCATATTCATGCAATTTTTCAATTGCAGCAGCAGCAGAATCTTTAGGAGGATCAAGAAATGTTAAATATCCAATCAAAGTCATATCGCATTCATCATTTACCGATATTTTTCCAACTGCATTAATTGTTGATTTTTTAGAAGCGATTGCAATAACTCTTAGTCCTTCTGAATTGAATTCTTCAACTCTTCTTAGAACCTTATCTATTGTTTTTTTATCAAGAGGCTTAACTTCCCCATCAATGTAAATATTTTTACATACACTAACAATTTCTTCAACAGCACCCTTGGTAATTAAGGTTACTTTATTTTGTTTTAATGATTTAACTAAAACAGACATTCTTTTTCTTTTGAAATCAAAAGGAATTTCGTCAATTTTTTCATAAAATCTTTGAAAATCCTCAAGTTCTTTGTTTTTTGACGCCATTTCAAAAGTCTTATTAATTATTGATTTATCAAGTAAATTTTTCAATCCTGTTTGATAATATGAATTAATAAAAGCATATTTTAAAACATGAAAATCTTCTTTGCCGGTAACATCCATATGACGTTCCATAATTACTTCATCTTGTGTTAATGTTCCAGTTTTATCTGTACATAAAATGTCCATTGAACCAAAATTTTGAATTGAATTAATATTTTTAATTATTGTTTTGGCCTTACCTAGCGAAACTGCACCTTTAGATAAACTTGCTGTCACTATCATTGGAAGCATTTCCGGTGTTAAACCAACAGCAATTGATATACCAAACAAAAATCCTTCTAATCATTGATTTTCTCCACCTATAACACCTTTAATTCCTAAAATTAAAAATACTATAGGAACAATAATAGCCATTATAATAGTGAACATTATTGAAATTTTTTTAATTCCTTTATCAAAGTTGGTTGGTATTGGTTTTTCATTGATTTTAGAAGCAACTTGTCCAATAATTGTATCATTGCCGGTTGCAATTACAATAGTTTTAGCAGAACCTGAAATAACATTGGTTCCCATAAAAGCTAAATTACTACGATCAGTTATTGCTTCAAATTCTTTAGAACTGTCGATGTTCTTATATTTTTCAATAGCATCGCTTTCTCCGGTTAATGAGGATTGAGAAATAAATAAGTCTTTTGCTTCTAATATTCTTACATCCGCCGGAATAATATCTCCAGCTGCTAAATACACAATATCGCCTACTACAATCTCATCCATTGGAATTTCCTTCATAATACCATTCCTTTGAACAAGCGAAGTAGTTTCAACAGTCTCAACTAATTTATCAGCGCTTGATGCTCCACGTTGTTCTTGAACGAAGTGAATAATTCCGCTTAAAAAAACCATAAATAAAACAATGATGACAGTCATTCCATCAATTCATGTTATCTTTTTACCTTTTGATGCTGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCGGCTTCAATTCTTTCTTCATCTTGAAATCCGTTTTGAGAGGTTTGATATTTTAAAAATAATTCTTCTTGACTACAATTAGAAGCTTCAACTAACCTTGTATTGATTTGCTTAATTTGTTTTTCTTTTCTTGAAGAATTTTTAAGTTTATTTTTTGTTATATTAACTATCATGTTATCTCCTTTTTATTTGGGTATTTATTATAGGAGAGCGAAATATAATTAAATACTAAAAGTTACTATGTAATTTTTAGTTAAATTGCTATATCGCTCACAACTTGGTAATTCCATAGTACCTCCATATATTTAAGTTTATAAAAGTATTTTAACATATTTCAAAATTAATTTCATCTAAACTTAAAAATTAAAAATTATATAAATCTTTTATTTTTAATTCATTTAAGTTTTTTGTTTCACCAATTTCAATAATCTGTCCATCTTTGACATAAATGTATTTCTTAAAATAAGTTTTTATTAATGAAATGTCGTGAATATTTACTAAAATTGTTTTTTTGTATTCTTGTGATATTGAAGACAATAATTCCAAAACATCTCTTGCGTTTGCTATATCAAGACTATTGGTAGGTTCGTCTGCTAAAATAATGTCAACTTTTTGTAATAAAATAAGTATCAACCACAATCTTTGCCTTTGACCCCCGCTTAATTCATCGACTCTTGTAAATAACTTATCAAATATATTGAATTTTACGAAATATTCATATATTTCTTGTTTTTGAGTTTTGCTTAAAACATGAAATCAAGAAAAAAGAAAATTTTTATATTTTGGATAAAAATGTAGAATGTTTTCATAGCAATTTAAACTTTCAATAAAATTATTGTTTTGACTTAAAAATCCAATGTTTTTTAATATTGCCTTTCATTGTTTTTTTGAAGCACTATAAATATCAATATCATTAATTTTTACATTGCCTTTTTTTACAAATAATTGCCTCAACAAAGCATTGAAAATTGTTGATTTTCCAGCGCCGGATTTTCCTATTATCGCTATAAATTCACCCTCTTCAATAGAAAAATTTATGTTCTCTAAAACTATTGGGCTTTTTTTATAATAACTTGCATCAACATTGATGAATTCAATTTTTTTCATATTTAAATTATATTTGGAATGTATTAATACAAAACAAAATATTGCAAAAATAAAAACATTAAGCGTGTTCTGCTCAATGTTTTTCAGCGAGGCTTGTATCAACCTCATTAATTTTTATTTAATAGTAATTTCAACATCCAAATTTTTTAATACTTGAATTGCTGTTTCTTTAATTTCTTTTGAATATTCATCAAATTGTTGTTGTTCATCTTCATCAAGTCTAATTTTTGGCAAGTGTGTATAACCATTCTTACCCAAGATTACTGGTAAACCAAAGTAAACTCCGGCATGTTTATATTCCTTTGGCAATTGAACTCCAAGAGAATAAATTCTTTGTCTATCATACAAAATTGCTTGTGTAATTTCAGCAAGGTTTTCGCCAATACCATAATTGGTAATGCCTTTACCGGCAATAATTTCAAATGCTTCTTTTTTAACCATTTCGTTTAATTGAGAAAGTCATTCATTTGTTATAGTACCATTAGCTAATCAATCTTTTAATGAACCCAAACCAATTCTTATTTTTGAAAATGGAATTAAGCATCTTGCTCCATGTTCGCCAATAACAAATCCAGTAATTGAGTGTGCTTTAATTCCAATTTTTTCTGAAAGAAATTTTTTCATTCTGGCAGTTTCTAGAATAGTTCCCGAACTAATAACTTTATTTGGAGAAAACTTGGTTACTTGTTGATAAATTGTTGCCATAATGTCAACAGGGTTTGAAGCAATAATTGTAACACCAGCAAATCCTGAATCTTTTATGCTTTGACCGATCTTTCTCAATAGTTTTGCATTATCGGTCAATAATTCCATTCTATCTTTTAAAACGCCTTCCTTTGGTCTAATTGATGCAGTTATAATAACAATATCAGCATCTTTCAAATCTGAATAATCTCCAGCCTTGAATGTTGAACCATTATTTGCCATTGAACAAGCAGCATCATTTAAATCCTTTGCTTGCGCAATTGCGACTTGTGGAAAACTATCAATTAAAACATAATCTGCTTCTATGTTTTTATTTAATGCAGCATATAAATATGAACTACCAACAGCACCAACGCCAATTAATCCTATTTTAGTCATTTTTTTTGCCTCCTAATAACTCTTTAAAATTTTTTGCCAAATTAAAATTGTAAGATTCCAAAGGCTTTCTTTCGAATGGATTCTGTTCATTTAAATAGGCAAATAAAATACTTGAATGATGTAAGAAAGACATTATTCAACCCAATGTACTATCGTTATAATTGTCAATCATGATTTGAATGTTAGGTATTTTATATACATTAACATGGTTTTCAATAAATGTTTCCATTACAAGATTACATAAATTATTATAAGTTAAATTGCTTAAATAATTCAAACCATCCGTCTCATTTTCGTTATTTTGTGAAATTTTGCAGTCCATAATAGGTTCTTGAAATTTTAAACAAGTATCAAAGCACTTAAATGGAGTGTTGGTGTAATTTGAGGCCAAAGTTTTGTTATCGACGCCCCCTAAGCAAGTTTCAATATAAATCCTATTGTTCATAGATGAAATGGTCTCACTGAAAAGCATTTGTCACAATTTTGCTAAGGATTCTTGATTTTGCTCGAAATAGCAAACATTTTCAAATATAAAATTATTATCATGAAGAATTTTTCTAGTAATTGCATATAAAACTGCTGAGTTTTTTTCTAATGGTGCATTTAAAAAAAATTCATTAGCCTTATGAGCACCAGATAAATATTTTTCAATATTAATTCCTATACAAGCAATTGGCAATAAAATTGCAGGTGAATAGCTTAAGAATCTTTCGACTGTATTATCTAAAATTGTTAAATGAGTATATTGATTTATCTTGGCATCATTAAATAATCTTCCATAATTATTGTTTGTCGTTACATAGATATATTTAGAAGCGCCTGCTTTGCCTAACTCTTTATATAATAAGTTTTTTAATTCTCTAAACATTATTAAGGTTTCTAAGCATTCGCCAGTTTGCGAGATTACATTAATAGCAAACTTTTTATTTTGAAGTTTAATAAGCAATTTGGCAACCTCTAAACTATTAAAATTTTCATTAACATAAACCAATTTAATTGATTCATTTTCTTTTCTTAAATCAAAGTGTAGATCAGCAAGCGCCTTAATTTGATTAATTATTGTTTGAGTTGCTATTATTACTAAATATTCAATACCATCTGACAATAATTCGCTTGATATCTCTTGAATTTTTTCATAGTCAGATTTCTTTATATTTTTAAAAACATTGAATACACCTAAATAATTTGAATTTTTAACCTTTGAGTTTTTTATATCTAACAAGATTTTTTGAACCGTCTCATAATAACGGTTTGGCGCACTTTCTTTAATTGCTTTTTTTAAATTTATGTCAATAATTTGCTTATTTTCCATAATTAATTCCTTTGTTAGTAAATTCTAATAATCCATCAAATTCTGCATCTTGACTATCTAGTTTAAATTCAAAAGGGTTTCTTAATTCCTTGGGGTGAATTCTTTTAAATGAAACTACTAAATCATTATTTGGCATTATTTCAATTACTTGAACTTCCTTAATATCATTGATTTTAAACATTAATTTTAAATCGCTAATGTAATAATCACTAACTTCATTTATACTCAAAAAGCATTTGCATCCTTCTTTTGTTTGCAAAATAATATTATTCTTTCAAACCTTTATTATTTTGGCTTTAAAAATATCTCCTATTTCATAATTCATTTTTGCCTCTATTTTTATATATATTTATAATAAAATTTCTAAATTATATTTTTGTATAAGTTATTAATTAATTTAATAATCATGAATATATAAATTTAAAATTTAATATATATTAATTATTAATGCTTAAATTATATAATATATAAAATTTAAGTTTATACATTTAAAATAAAATGTAAAAATTAATATAATTAGTTTATGAAAAATAGTTTTTATAAATGCTTAAACATTGCAACATTGACAGTTTCCTTGCTTTCAACTAGTCCTATTTTGATGATTAGCTGTGATGTAAAGAAAGATGTTGATTTATTGCATTTTAAAATGACTGTTAATAAACCTTGAAATGGTTCAAAAAATGATGAATATTTTAATCAACTAATCAATGATATTAAACAAAATAATAAGAATTTAAAATTTGATTTTAACGTTTCATATATTTTAGACAATGTCGATATTGCCTCTGCTATTAAAGCTAAAAAAGCAAATATGGCATTTATAACATCAACTTTATTAAAAAATTCATTGCTAAAAAATGATGTTATTCCTTTACTCCAAACAAAAACAAGGGCTTTTTATTTCGACAAATTAGATGAACAATATGTTGATGGTTCTTGCAATGATCCACTAAGAAAAATAGCAAAAATAGCAAATGATTTATTTAACAAAAAGCCTTATAAATCATGAACAGATAACGAATATCAATGAAATGGAAAAATTTATGAAAAATTTTATGACAAAGAAGGCGTTAATGTAAATTATCAACGAGGGTCAATCATGATTTTTGGAACCGATTCAGAAATTAAATCAATTAAAGAAGCATGAGAAAATAAAGACTGAAATGCATTTAGAAATTTTGGAATTGTAATCGGAAAAAAATCAAGTGGAACAAAATACATAGCACAAGAGGATTTATTTAAAAAACATTTCAATTTAGCAAATAATAAATTTTCTTCTTTTGCAAATGATCAAATAAATAATGCTAATAAATACATTACTGAAAGACCCAGAAACATAGGTAAGGGTGCACTGTCAAAATACCACATTGTTTTTGATGAATTTGCATCCTTTGCATATACACACAATCTAAAAAAATCAAAAAGCATAAACTATAATTTTTATTGCCCTGATGGGAATAAAAAGATTGAATTATTAACAGTAACAGATCCAATAAAATTTAATGTCTTAGTTGTTGATAAATCAATACCAAAATCCGCTTATCTTGCTTTTGCAAATTCAATAATCAATTTAGCAAAAGAAAAAAAAGATACATACGGACCAAATGTTGGTTTCAATGAATATGAACTGATTAACGAATCTGACTATTCAAAATATTTTTAATTATTAGCAATTAAATTAAGTATATAATTTTTTTATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGCATCCTCTTTTTAGGAGGATTTTTTATGCTAAAATTTAAAAAATATTTACTTATAGGGGCAACGATATTGCCTACAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGAAGTGAAGCAAGCAATAAAGATAATTTCTTTTATGAAGTTATTAATAAAATTAATGAAAATAAAAAAGACTATAAAGTCAACAACGCAACGTTACATTATTTAGACGATAATGTTGATTTTATAAAAGAATTACAAAAAGGAACTGCTGATATTGCATGTTTAACACCAACGGTGTACAATCTAGAAAAAGATAAATCAAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGATTAGATGATGATCCGCTAAGAAAAATAGCTCAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGAAATGATAAAGAATATAAATGAAATGGTTCAATCTATGAATCATTCTATGCAAATAAAGACAAATTAGTTGATTATTATAGAGGATTGGTTGCTATTTGAGGAACACAAGAAGAACTTAAAGCAATAAAAGAAGCCTGAGATAAAAAAGATTGAAAAACATTTAGAAATTTTGGAATAATGCACGGAAGTAGTTCTAGTGGTTCAAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAATGAAACAAAATATATTAAAGGCAAGATTAAAAATATTGGAAAGGGTTCATTTTCTAAATACCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACTCACAGCAAAGGTAGCACAATCTATAAATTAGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACCGAACCTTTAAAATATAACATTTTTGCTACATCATCAAATATTCCAAAAGAATTAAGAGAAATAATTGCAAATGCAATGGTTGAAGTTTGAAGAGAAGGCAAAGATACATATGGACCTACTGTTGGATTCAATGGTTATAAGATAATAAATGATCCAATGGAGGAAGTTATATTGCCTTATAAAAATGTATTTAATATAAATTAGTTATGAATAATATTTCTTTTCCAAAATCTTTCTATTTTGAAAAAATAAAATACGATAATTTAACGTTAAGGGGACCAAAAAAAATTATTTGAAGAATTTTAGGTCTCGTTTTTGTTTTAGTCTTAATTGCTTTGTCAATTTATTTTATTGACTTCTCGTTTGCACCAAACGGAAGAAGCGTTTTTAGTCAAAATATAATTGATTTTTTTAGACCACATTTGATTAGCAAATTAATTCCTGGTCAAAATTTATTTCTATTATCAATTAAATATTTATGAGAAAGCATTAAGGTTGCTTTTTTAGGAACAATTATTGGGACATGTTTAGCATTATTTACAGCATTTTTTTCTAATTATAAAATGAATAAATATTGAATAGCAATTCCAATAAAATTCATAATAATAGTTTTAAGATTACTACCAGAATTATTCTTTATTTATTTATTTCAAATTTCAGCAGATAAAGCGTTGGCTATAAATTTGATTTTCTCTTGATTTACTTGGCTTTGATTACATGAATATTTTTCTCAAGTAATTGAAAATGCAAATTTTGAGATATTCTATTGACTAACAAAAATAAAACAAAATAGATTCACTGCATTCTTTAAAGAAATTTGGCCTCAAATATCTCCAAAAATTCTTAATTATATATTTTATGCTTTTGAATCAAACATTCGTTGATCTACAATTCTTTCAAAACTTGGTTTTTTAGGAATTGGAATATTGATCTATCCAAATTCAATCAATGTCAATTACTTTCATGAGCTTCTAATTCCACTGCTAATATTAGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTGTTGTTTTATTCAAGGACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAGCAATTGCAATGGGATTAATAATAACTATCTTTGCATGTTTATCATTAAGAGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAAGAGTTATTTAATGGTGAATGAAAAAATGTTTTTCTTGGATGAAAAACAAACAGTAATTTTGGACTTCTATATATGCTTTTGCAACTTGGGGCATTGGTGCTTTTAAGCATTTTACTAACATATTTTATATCGTATTTTAAATTGCTATTGCTTTCAAGACAATTATTCAAACGCTCATATTGAATTGAAAAAATTTGAAACACTATTTTAAGATCAATTCCTGTAATTTCATTATTTTTATTAATCTCTAATTTGTTTAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTAGAGAGCTCCATTAAAAAAATCTCGACAAACAAGATAAATGAACTAAAAATAAAAGGATATTCAAAATTTTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATACGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCTGATATTGCGCCATATCTATGAATTGAATTTATTATTATTGGATTAATTAATATGGGTAATTATTTATATAGATTAAGACTAAATAAAGGCAGCATTAAAAAATAAAATAATACTTATTTAAATATCACTAGATAAATTTATCTCTAGTGATATTTTATTAATTACAAACATTGAAATTTAATTAATTAACTTAGATCTTTAAAAGAAATGTATTACTTAAAAATTATGTAATTTATATAATGTTTGTTATAATAAATATTAGACCTAGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCATATAAAAAAAGTTTGTTAATAGCAAACTTTTTTTTAAATTCAATATTTAAAAAATTTGCTTTGTTAGCAAATTTTTAATTTAAATTAATTTTGATATTTTTTGGCAACATATTCTTTAATTTGATCAACGCTATCAAGCTTTTCTCAACTTAAATTAAGGTCGTTTCGTCCAAAATGTCCATATGTTGCAGTTTGTAGATATATTGGCTTTAATAAATCTAATTTTTTTATAATTTGTTCAACGCTTAAATCAAATACATGTTCAATAATGTCAGCAATTTGTTCATCATTATATTGTGATGAATTAAATGAATTTATATAAATAGATTGAGGACGTGGCAAGCCTATTGCATAAGACAATTGAATTTCAATCTTGTCTGCTATCTTAGCAGCAACTAAGTTTTTAGCAATATACCTTGCCATATAAGCAGCGGACCTATCTATTTTTGTAGCATCTTTTCCCGAAAATGCTCCTCCACCATGGTGAGCAAAACCACCATATGTATCAACAATTATTTTTCTTCCTGTAAGCCCAGTATCTCCAACAGGACCTCCAATTACAAATCTACCAGTTGGATTAATCAATAAATCATAATCCAAATTGAAATTAAATTCTTTTAACACATAATCAACAATATTTTTCTTTATAAAATCTTTAAATTCTTTTAAATTTGCATTTTCAGTATGTTGAATTGACATTAAAACTTTTTTAACAGTTATTTTATTGCTTTCATCATCATATTCCAATGTTACTTGGCTTTTCATGTCACTTTTTGCATATGGAAATTGATTTTGTCTTCTTAAAAATTCAGCGCGTTTAAGAAGCTCATGTGCTAAAACTATTGGTCAAGGCATATAGAATTCATTTTCATTAGTTGCATATCCAAAGATAATCCCTTGATCACCAGCACCCAATTCATGGTCTTCTTCTTTATTGACACCCAAAGCAATATCGGCACTTTGTGGAGCAATACCATTAATTATATTAAAATCATTTTCATCATATCCTAATGGTTTTAATACCTTTCATGCTTCTTTAATTGTGTCAGAATATGCCTTTGTAGTTATTTGACCGCCTATATAAATGATGTTGTTGTTTGCTAAAACATCACAAGCTACTCTTGCATATGGATCATTTGTCAACAAACTATCTAATATGGCGTCACTAATTTGGTCACATATTTTATCAGGGTGACCTGCCCCTACACTTTCTGATGTTAATAATTTTTTCATTTAAAAACCTTTCTAACACAGAGAAAGGTTTTTATTATCTTTTTTTGTACATGTTAGATATAAATCCCTTACCATCCACCTTGCTTTATTTAAGTAGGTTGGCATGCCTTAATATGGTTAACTGGCAACTCTCTATGTATTTTTTGTTTTATTATTATACTAAAAGTTTAGTATATTATTAAAAATTAATAATTATAAAATTATAATGTTTTCTTTTGCTGATTCATCCAAGGTTTTTTGATCTCATGCAGATGCTTGAACTTCACCAATATGATGTTTTTCTAATAGAAACATTGCAATTCTACTTTGGCCTAGGCCACCACCAATCGTATATGGCAATTTGCCTTCTATTAAATCACAATGATATGGACTTAATTTTTTAATTTCTTCTTCGGTTTTATTTGATTGTTTAATTAAAGAATCTTTATCAACTCTAATTCCCATACTGCTAATTTCCAATGCTTTTTTATTTACTGAATCATAAACAATTAAATCCCCATTCAACATTCAATCATCATAGTCGAATGCTCTTTTTGAATGTGGAATTCCATCAGGCAAATTGTATCCTATTTGATAAATAAAAACAGAACCATATTCTTTGCAAATTGCTTCTTCACGTTCTTCGGGACTTAGGTTTGGATAAAGATTATACAAATGGCTAGAACTAATAAAATAAATATTTTTAGCTAACTTTTGACTCAATACAGGATAAATTCTATTAATTGATTTTTCAGTATTCTTTAATGCTCCATAAATTAACTTAACAATATGTTTTAAATATTTTAAATTTCTATCTTCTTTTTTTATAATTAACTCTCAGTCTCATTGGTCTACATATATTGAATGTTTATAATCTAATTTTTCTTGTTTTCTTATAGCATTCATATCTGTATATATTCCTTCATACAATCCAAATTCATACTTTTTCAAAGCATTTCTCTTTCATTTAGCAAGTGAATGAATAATTTGTAATTCATCTTCAACTTCGTCTGCAAAAAATACAACTGGTTTTTCTCCGTTCAAACCGTCATTTAAACCACTTTTAGTTTTTACAAACAATGGCGCTGAAACTCTGACTAAATTCAATGTTCTATGAAGTTGATGTGAAAATAAATCTTTTAATTTTTCAATAGCAAATTGGGTATCTTTCAATGATAATTTTGATGATGGCATATTTTATGCTCCTTTTTTAATTAATTTAATAAATTATAAATTAATATAACTCTTAAATATAAATAATTTTTTAATACAATAAAAATCAAGCCTAATGGCCTGATTTTTATTACTTATATTGCCAAATTTGAAACTATTTCTTTTCAACAGTTAGGCCATATTTAGCACTTACTGATTTAATTAAAGCATCCATTGGCAATGCACTATTACGTAATAAAGCATCAAAGAATTCTTTTACATATTTATGATTATCTTTTTCAACAAATTCTCTTCTTGTTAATCCTAGTTTCTTTTGAACTTTTGTATATAGATCCATCATAACTGCTTTACCTGAGTTATATGAGGTTGCTTGACCAACATATGATAGGTAACGTCTTGATTCTGATGTTATGTCACCAATTCCTAGTGATGAATTCTTCTTCATGAATTCTCTTACTTGGTTGATTGAAGCACCTGCTGGCAAGTCTTCATGTCCTTTTACACCTTTACCATGGTAAGCAGTGTCTAATGCTAAACGCATGTTACGCAATTGAGCTTCGTTTAATGCACCTTGGTATTGCAACAAGTTAGCCAATTTTACAGCATCCATTGCATGCTTTTTATCTTGGTTTTCATATTTATTAACTTGTGCAACTTTATTTCAATATACACCATTGTGTAAGTCTTTAATTTGTTTAATCATTTCATCTGTAACTTCTGCTTCTGTTTTAGCAGTAAAGAAGTTTGTTATACCATGTGCTGTTGAAAAGTCAACTGGCATTGCATATAAATCGCCATCTTTATTGTCATAATCTGGTGTGCCATAGAATCCAGCTTCAATTCCAAATCATTCCATAAATAGAGCTCAACCTTCAACGTATGAAGTGTAGTGGAATACATTTCCTAATTTATATTTACCAAATTCTCCAACTGATGATAGATACTTTTGAGCATACATTAATTGGTTGTGGTGTCCCATCATTGATTCATGGTTTGCAAATGAAGTAACTGATCATTTTGGTAATGAGAAGTATGGGTCAACATTAAACATAAATTTTTTAATGCCCGAGTTGTAAGCTCCAACACCTACAAATTCTCTTCTAGAAAAGTCATATGTTTGAATATCATAATCAGGAACTTCATTTGCAAAGAATTTACGTCCATAATTTTGAGTAGTCTTCTTAAATTGATCATATGCTTTAAATGCTGACAATCCACCATAATAGAATTCTTCGTTTGTTATTGAACCATGTTTTGTAGCTTTATTTTTTTCGCTTAATAATTGATCATAATTAAAGTTTGTTAATTCGCCAACGTATTTCTTTAAATTAGGATCTGTTTTAAGACCATTTTTTATAGTATCTGTTCAATAGGCACTACCTTCACGTCCAAAGAAGAATTCTTCATCATTTAATCATTTAGTAAATGAAGGAATATCAATGTTTCCTTTTTCGTCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCGTATTTAATTTTAGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAACCAATATTATTTACTTGATCATCTGTTAAATCAGAAGTTGTAGCCATCTTTAGAATTTGACGATACATCGATTGACCATTTACGGTAGCATCTCCTTCAGCAAAACCCAAACCAACATTTCTTTGATTTAAGTCTTTTTCAGTTAATCCTAAACCATATAATTTAACAATTTCCCCACTAGCATTTTTTGCATCAATAGATAATTCTTTTTCATTAACTTTATTTTCTTTATAAAGTTTTAATTCTTTAATATCTTCACCCAATCCATGAGTTGCAGCATTGTAATATGTAGTTGCATAATATTTTAATAATTCTTTTTTGGCTGTTGGCAAGGTAGAAGAATTAATTAATTCTACAACTGAAATTTCGTTTTTAGACTTATCATTTACAAAATTAGTTAGTTGTGTTTGAAATAAACTTCCCACAAAGGCATTGATTACCATTTTTGAGAAAACTTTCGACATAACAACTTTATGTTGCACTGCTTCTTTGAAGTTTTCTAATAATTGAGTGTAAGGTAAACTGCCTCATCTTCCTAGTTGATTACTAAATGCATTCATTGGATATACAGATGCATTTCCCCAAGAAAATGTAGGATTTAGTAACCTTAATTGATTATCGTGATGTCCTTTTGCAATTTCTCAATCATATTTTACGGCATATTTTCATGCTAAAGAATCGCTTGTTAGTGAAGCGTCATTTATTTTTGAAATTTTTTCAATATATTTACCATAAATTTCAGAAATTTTCTTTGATTGTTCGGCAGTAGGAGCGGTTATTCCATTTAATTTTTCTTTCTTTAATTCAGATTGTAGATCATCTTTTAAATCCAAGATGTGTCTTGCGGCTGCTTCTTGACTAGCTGGCGAATTATCATTTTCTTTTGAAAGTAAATTGCTTAATTCATCGCTTTTTTTAACGCTAGCATTATATTCCTTTTCTAGCGCATCTAATTCCTTTTTATTAGCTTCAATTTCTTTAGAAATATTTTCTTTTTTTGCTTTTAAATCTGCAACTTTAATTTCAAGTTCTGACTTTGTATTAGTTTCTTCAATGATTTTATCTTTTTGAGTTTTTAATTCTGTTTGAAGACCTTCAAACTTTTCTTCTTCTTGCTTAATTTCTAAATTCTTAGCTTTAATTTCTTCTTTTTTTGATGATATTTCATCATTTTTTGAACCATCAGAATTTTGTTTTTCTAATGCTTCTAAATTCTTGTTTAATTCTTCCAAAGCTTTTCTTAATTCAACTAATTTTGTTTTAGATTCTTTAATGTTATTTTCTATTTCTGTTGATTTTTTCAAATAATTTTCTATTTTTTTTCCTGAATTTTTAAGCTTGCTTTTAGAAGACATTAGCTTAGTTTCTAATCCACTTAATTGGGATTCAAGATCATCATTTTTTTGTTCTAGTTCCTTATATTTATTAGGAATTTCAACAATAGCCTTGTTTGCAGAATTTAATTCTGCTTGATATTTAGCTGTTCTTTCTTGTTGGATTTTATTTAAATCTTCTTGCAATTTTTTAACTAATTTTTCTTTTTCTTCAATTGTTTTTGAATTATTTTTTGTTTCTTGCAATTGCTTCATAAGATCATTAATTTGTGCTTGATCTTTTTGAGAATTTGCTTTTGCTTCATTTAATTGCTTTTCTAAAGATTCAATTTTTTTGTTTAATTCATCATTATTTGTATGTGTACATGATGCTGCCATAGGCATTAAGGCTGCTACACCAAAAATTGAACCTAATGAAATTAGAATTCTAGTTTGTTTTTTCATTTTTCCCTTCTTTAACTTTTAACTTATATTGTTCTTTTTTACAACAAAGTTTTTATTTTTATATAGTCATAATTTTAGAAAAAAAAAAAAACGCTTATTTTGTAATTTTTTTGCATATAAAATACAAAAAAAATTAACATTTTCTTATAGTTTTAAGTACAAAAAAGCATAACAAAAGCACAATAAAAAATTTTTTGCTAACTTTAAATATTAGAATTTATGCTTTATTTTTGTAAACTATATATTTTTTAAAAAAGTGGATGAAAATCAAAAAATGATGATGCTTTTTTTAAAAAATCTAACAAAATAAAAAATTTTTAAAAATTTTGAAACATATTTAAAAATAGTATATAATAATACACGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTTTTATATCTTTTTTTTAAAAAAAATAAGGCTTTTTCAAACCTTATTTATATATAGATTAATATAAGTTGCAATTAACGATTATTATTTTTAAAAATTTTTTTACCTAATTTAATATATTTATCGACTTTTTGATTTAAAACCTCAAATGCTAAATTATAAATATCTTCATTATAAATATCAACACCAGCATCTTTTAAAATGTTTGCAGGTCAATCGATATCTCCGGCACTTAAAAATTTATTTATATAGTTTTTTAATGCTTCTTGGCCTTCTTGTTTGTATTTTTGGAAAAATACATTTGCTACGATATATCCTAATGCATATTTATATACATAGAAATAATAATAGAAATGAGGAACCATTACGCTATAAACATTTCTAGGATCGCCTAGTTTTATTGATTTTGATGTAGTCGAGTATTTCTTTTCGTTTTCAACATATAATTTTTCTATTTCTTCATATGTATTTAATGGTTTATTTTCATCTATTGCATTATATAGATCGAATTCAAAATTAGATCACATCGTTTGACGCAAAACTGTGCCAATAAAATCATTAATGCTTGAATCAAGTAGATAAAATTGTTGTTGAATATTTTTTGATTTGCTTATTAAATAATCATTTAGTAATAATTCATTAAATATCGAAGCAATTTCTGCTAAAAATATTGGATAAGAGCTTCTAAGTGGAGATTGGCATTTATTAGAAAAATATGAATGCATTGAATGTCCCATTTCGTGGCATAATGTATTGACTGATTCCAATGTTCCATCAAAATTCATCAATATATAAATTTTTTTCATTCCTGCTGCCCCGCCTATAGAATAAGCGCCGCTTCGTTTAGATGGAACATTAATATAATCAATTCATCGTTCATCAATAGCTTTTTTAACAACTTCTGGATATTCATAAGGCATTATTGAAATAACATCTAATAAAATCTTTTGTGCTTCTTCTATTGTATATGAAGATTTAACATTAACTAAATCTAAAAAGTAATCTCATTTTTCTAATTTTTTATTGAATTTAGCTTTAAAAAATTGTTTTTTAGCATTGTTATATTTTTTAAATATATACATATGTTTTTGAACTGCATCATAAATAATGTTCAATAACTTTTTATCAACATGATCTTGCAACAAAATAGATTCAAGAGATGAATTATATTTTCTATATAAAGCATTAACTGATATTTCTTTAATATGTTGATATAGCAATCTCGCTAATGATTGTTTGTTTTTTAAATATCCATTTAAATAATTAAAATAAACATCTTTTCTAACTTGTTCATCTTTATTTTTTAATAAAGCTATTCTATTTCCTTCGGTTATTTTTATTTTTTTATTTTTAATTAAAACATTGCCAAAATCAACTTCACTATCGCTTATTATTGAAAAAATATCTTCAGGTACAGGCGAACCACTTGCAGTATCATTTAAATAATTTTCAACATCATCCGACAATTTATGATCAAGTTCTTGCAAAGTTGCTTCTAGGTCTTTTTTTACTTCAATTAGTCTGTCATCATTAATTCATGTTTTGATTTTGTCTTTATGTTGAAAAATTCTATTGGCTTCTGAACCTAACTTTTTAGCATATTCTTGGGATTTTGTTTCAAATAATGAAATTGTTTTATTTATTGTGGGATCAACAATATTTATATTTAATTTATTATCTAAATAATTAGAAATTCTATTGCCTAAAATTAAGAATTTCTTTGAAAGCTCAATATATTCAACATATTGGCCTAATGAATTGTATTTGCTATCTTTTATTTCAATTATTTTTGAATACAAATCAAAATATTCATCTTCTAATTGTTGATAGGTTTTATTACCTAATATGTCTTCAATATCTCATTTATATTTTTCTTCAACGTCACTATATGAATTGTATTTTTTTAACATTATTCCCCTTTATTAATATAGATTAAACTTTTTATATCGTATGGCAATCTTTCATGAGAATTAAAGCCATCTAATTCCATTAAAACAATTATTCTACTGATAATTACACCTTGTTCAGTTAATAATTTAGTAATTGCTTCCAATGTACCCCCAGTTGCCAAAACATCGTCAACTATGACGGCTTTTTGTCCTGGTTTAACTAAACCTTTTTGAATTTCTAAAATATTTCTACCATATTCAAGTCCATAATCGCAGCTTATAACTTCGCCGGGCAATTTGCCTTTTTTTCTTACCATAATAAATGGTTTTGATAATTTAGCAGCTACTGGTGTTCCGAATAAAAATCCTCTTGCATCAGGACCCACAATAATATCTGCACCTTCTGCTAATTTAGCCATTTCATCAATTGTATAATGTAGTGCTTGTCCATTGGCTAATAAAAGCGAAATATCTTTAAATTTGACGCCTTTTTGTGGAAAGTCATCAACAGTTCTTATATACTTTTTTAAATCCATAAATTTCTCCTGATAATAAGTTGATAAATAACGAATAAATTTTAATATTATTTACAATTTTATTTTATAAAAAAATGATTTTATTTCAAACATTTGAATAAAAAACTAGTTAAAATAAATATATTAATTTGCTAAATTTTTTTTATTTATTGATAAAATAAAATTAACTTAAATTCTTTTATTTTAAATTTTAATAATTACTCGGAGGATAGAATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTAGATGAAAACGGCAAAAATTGTTATGTTTTAGAAATAAATAACAAACTATTTGTGATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGATTATTAATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAATAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCGTTTGGCGGGGTAAATGTAACTCCTTTTGATCTAGCCGGAGGATTACCTGGACAACTTGGGTTCAATTTTGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAATAAAAAGGAATTACAGCTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGAGTTTCAAGAAAAATTGAATACAAATTTAAAGAAACATCAGATAACCAAAGAATTATTGTTGGTTTATATGATGAAGATATGATAACTGCACATGAAATTTTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAACCCAAATTCTCAATTTCCAGAATTCGTTGAATATCAAACTGCAAATAATGTTAAAAATGCCGTTATTCTTGTAACGGGCGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGACAATGATGTTTATTTAAAATTGAAAAATGATGATGCAGTCATAATAATTGCTCCACCAGTTAACGGATTGGAAGTAAATTACGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTTCTAAATTAGATATTTTTAATATTATTAAAGCATTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCTAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCTGAATTCAACGATACAAATTTAATTTCTCAAAAACATGGAATTAGAAATGTAGGTGATGTTATTATTGATGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACTTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTTGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAAAGAAAAATCGCCAAAACTATTGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAACTTTTAAAAATTATAATTATAGTGATAATTAGGAATATTTATGAAAGTAGCAAAGGAAAAACAAACTAAAATTAAGAACCAAAAACAATACTTTTTTTCAAGAGACAGGGTGCTTTTATGAACAATAATACTTTTAGTAACATTGTTTTTTTTAGTTATATCTTTCCTTAATGTTCCTTGATTAACAACATTGCATTCATATTCATTTAATGTATTGTTTGGAATGTTCTCAATATTATTTTATTTATTAATTATTCTACTTTCAATATGAAAAATCTTTAATTTAAAATCAACGTATAAATATTGAATATTTAATTTTAACCTTGGAAGATTAATATTCTTTTTTATTAATATTATAATTTTGGGTTCTGTTATTTATTACACCATTCATCAAGTTGAACATAATTCAAAGAATTTTTTTACTAAATATTTTAGCAGTTGATTTGTTGATTTTAGTAATAGCCAAAACTATTTACTACCTCAAAAATACACCCCCGGAGTGATTGGAACATTTATTAATTCAATATTAAATATTCCTGGAAAAAAACTCGGTTTCTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGCTTATCTGTCATTTCATTTTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAGGAAGAGGATATTGAAATAATTGATAACAAAGAAGTCGTAAATAGCAATATTCAAATTTTAGAGGCTAATTCTAACTCAAATAAAAATATAGACTCAGCACAAAATTTAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGACGAAAAAGAAATTGAAGAAGATTCAAAGCAAAAAGACAACAATGAGCTAGAGCCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGGTCCTACACTACAAGAACCAGATAATTCAAATAAAAATAAAGAGGTTAAAAAAGAAGATAAGATCTATAAAATCATTGAAGATGAAGAAAATTTGTTCTAATTAAAGTTATTCAGTTTTTATAAACCTTATTTAGAAAAAGAAATTTCAATAACAAAATAATTTAAGCGTTTTAGCTATAAACAAAGAGCATTTAAATTGCTCTTGTTTTTTTATATACAAATTATAAATTTACATAAATATTTTTATAAAGGCTATTTTTATATATAATTTCTAAGTAAAAAATTTATGATTTAGTTTGCTAAAAAAAGCAAAGTATAACTGATAGACTTCATATTTAACAAATAATAGTTTATTAATTTGAAATAGTTTTTTATTTTTTTAGATGTATTGGTTCAATATATCTTAGTTAACTTTTAAATATACACACCCAAGCACAATAAATAATATAGAAAGGTGCTAGTAATGAAAAACAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCATTAACATTGATTGCATCTGCCATCACGATACCCGTTGTAATTTCATCCTTAATTATAAATAAGAATAATTGGGCTAACAAATACTATAGCCAAAGATCAGAGGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCTTAGAGGATCAAAATAATTTAAATAAGTTATTGAATAAAATTAATTTAACTAGCAAAAATAGTTCTGCTGATTTTAAAAACTCATATAATTTGCTTAACAAGACAAATCTTGATATCAACAAAAAGATAAATATTAACAAAAAAGAATTTGACAAAACTTATGCTGATATTTTGCTTTTAAGCCCACAGTTGCAAGATTTATTCAATAAAATTATTGATTTGAAATTGAACAACAATATAAAAGAAGATGTTGATGGACTATTACAAAGAGTTGAAAAAGATCTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGATATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTAAATTCATATTATAAATTAATTAACAGCAAAGATAAAACATATAAAGGCATTGTCTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATGGCAATGAAATAAACCATCTTAAAAAGAATTTTAAAAATTTCCAATCAGTTGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAGGAAATTAAAGAAGAAATTGTTGAACATAGAAAGCTGAGCCAAAAGATAAAAAAAGATGGAAATGATATTTTAAATAATGATTTTGATAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCGAAATCATTTTTATTAATGTGAAATACCTTCACTAAATTAGATATGTTAATAAAGGCAAAATTTATTAATGAACGCCCTAAACAAATAAAAGAATTCCTAAAGCAATATGAAGAAAGACAAGAATACTTTTATAGAATTAATGAAAATGTTCCAAACCTTTTTTACACATGAGCAATTTATAATAACAAAATTAAAATTATAAAAGAATTCTATTTTAAAGATAAAAAAGAAAACGAAGAATTTAATTTATTTTCTTACAATGATACAAATGTAGACAATCAATTAAAGTCATTCCTTGACGAGGAAAGAGAATTTAAAAATAGGCAAGAAAGACTAGTAAGATTGTTTTATGACAAGGTTATTCAAGACCCAAAAACTAACGAAATTGATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCGGAACAAGATATTAGATATTTACACTACCATTTTGGAAGTCTCCTAACTCAAAAGGTTAATGAAATAATTAGGCATTATAATCAAAAAGCAAAAGCCTTATTAGAAAAAATAAAAAACAATAATAGTTACGAAACACAAAAAAATAGAATAGAAAAATTACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGCGATTTCAATGATTACGAAAAATTCTATTGGTGAACATATAAATTTCAAAAAGACAGCAATGGTGATATAATCACTTCATCAAACAGCGAAATCCCTGATTTTTATAAAAATGAAATTGAAGTAGCCCAATACCTACATGAACTTGATGAAATACTTGATAATTTAAAAAACATTTAAATATTTGTTTCTTTAATAAAATGGCATTATATGTGCCGTTTTATTTTTTAATAATTAAGTCAAAAAATAAATTAGATTTTTATAATGCTTAAAATAAGTTACTTTTTAATTAAAACTTAATTTGCTTTTGCAATAAAAAAACTAAAAATAATAAAAAAATTTTACATATTTGTTAAAATTAAAATGTTTTTTTACAACGAATTAAAAAAATAATACTTTTAGAAAAAATTAAGGAGAATATTATGAAAATGAGAAAGAAATTAATTGCTTTATCTGTGGCTTTTGGACTTTTAGCGCCTATTTCAGGAACTCTTATGATTTCATGCAAAAACGAAAAAAGTAATGCTGAATATCAAAAAGAACTGCAAAATAAATGAACAGAACTTACGAATCTTTTAAATTCAAATTCATTTAAAGAAGTTGAATCTAAGCTAGATCTTTCAAAATTAAACGCTCTTAAAGAAGAATATAAAACAATAAACAGCAAAAGCAATGTTTCAACATTGAAGAATTTTATTGCAAAAATTAATGAAACTACACAAGATATAAATGCACAAATTTCAAAATTAAAAAACGAAAACGGAAATACAAACCCTGAAAACAATAGCCAAAATGATTTATTAAAAAAATTAAAAACAGAATTGGAAACGGCAACTAAAAAACTTAACATGCTACTTTCGGCAAATGAAAGTAAAAACATCTCAACAAAAGAAGAAGCTAAAAACATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAACAAGAATATAAAAATCAAATTCAAAAAGTAAATGAAACTATCAATAAAATTGAAGAAGAAATATCAAATTTAGAAAAAGGCCCAAAATCAAAAGAATTTACTAAATTAAAAGAATTAAGAGAAAAGCTAACAAATTACTTAAATAGTAATTTCTCTGAATTTGCTTATAAAGATTTAAGAGCTGAAATTAAAGAATTAATTCAAAAACAAGAAAACGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTATGCAAATAAATGATATTTTTTGAATTGCCAACATCAAAAAATCAATACTAGATAAACAATTTCTTTATTTAGAAAATAAACCTAAATCTGAACTAGACGTAAAATTTTTAGCAGCGTATGATGAATTAAGTCAAACCTTGAATACCTCGATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACTAAAATTTCTGATGTAGAAAAACAAAATTATATTGAAGAAGTTAAAAATACTATAAACTCTTTGTTGAACAAATATAATAATAATATAAATAACAATGAAGATAAAATTTCTGAATTAAATCGAAACATAGAACAATTAAAAAAATTAAATCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAATAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCCAAAAAAGCAATTTTACTAGAATCATTAAGAGATACCGTTTCGGAATTTCTTGAATTTTACTTACAATTAACCCATTATCTAAGAATCTTTTTTGATAAAAATAATGACATTATTAAATTATTACCAACAGATTCACAAACAAAAATTGATAATTATCTAGACGAAATTAAAAAAATTGCAGAAAAAAATAGTCAAACAAGAGGAAGCATACCAACATTTGACGAATATATTTCAGCCTTTAAGGCACAAGAAGAAATTTTTAAAATTATAAAATCTAGTAATCTAAAATCAGCAGATGTTAACATATTAAAAAAATTGACAGATAATTTAAAAGAAATTAAAAAACAAAAGAAAAATGATTATGGCACGATTGTTACTTCTTATTTAAAAGAAAAAATAGAAGAAACAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATGTTGAAAGTCAAAATATTATTGAAACTTTTGCTTTATTGCAAAAATTAGAACTTGAATTTGCTTATATTAATTGAACTGAATTAAATTTGATTGCTAAAATTGAAAATAAATTAGAATTGTTAATAAAAATCAATAACATAAATTCAAATGAAAAAGAAAAAATATTAAAACCTTGAACAGATTTGCTTAATAGTCCAAAAAAACCAGAAATTTTCGAATTAGAAAACATCTTAAAAGAATTAAGCAAATCTTTAAATGAACTAATTAACAAATAAAAGTTATTTATTTAATAAAAACATTGTAATAATTTTGTGCAATGTCTTACTACTTTCTCAATAGAAAGAAACTTACATTACACAACCTATAAAAATAGTGTATAAAAAACAAGATAAAAGTTCTTGGACAATTATCTTGTTTTTTATATTATTAATTTTGTTGCTATGAATATGCAATTTATTGGGCTTTAATTGTTATTTCATCGATGGTTATAGGATTTGCCGTTCCAAAATATTTATAGCAGGAAATTTCTGAGTCATAAAATTGAATAAAACCTTCAAATCTTAATTTCCCATCTTGTTCATAAATTCCTTTAGTTTTTTTGGCTCCCCCTAAAAGTGTATTAAAATTTACACTTACTTTGCTAAATAATTTAAATAAAGGAAGACTTTTATCAAAATATAATTTTCTTTCATCACGAGTAGTTTTATCGGGCAAGAATGTTTTTATTTTATAAACACTAGCTTCTCTCGTTTTTTCAAACATATTATCAATGATTTTATTAAAAATTTCTTTTTTAACTTTTACAAGTGAATTGTCAAAAACTTTCTTTTTTTTAGTAATAAATTCATCATTAAAATCATCCCTTCCAAACCTACTTCAATGTAAATATTCAATTTCGCTTCTGCTATCTTTGGTAATCTCTATAACATCTTCTTCCATTATCTTTCGTTTTATTTCATCATTTAATTTTGCATCTTCTGATTCAATATATTTAAATATTTCTTTTGAATAATTAGCATACATATCAACTATTTTATAAATATTTTTAAGTTGATTTATATATTTCTTACAATCTTCTTTATTTCTTGCATTATAGGCTTTTCTATAATATAATCTTTCTTCAGTTCCCGGATATTCACCATAAACTTCGTTATTAACTTTTTTATTAATTGCGCTCTTTATAGATTCATTTGATTTAATTAATTGTTCGCTGTATGTCTTATTAAATTTTAGAGAGCAAATTTCATCTAGTTTAGATTCAATTTGCTTCTTTTGGTCTTGAAATTCTTCTTCAATTTTTAAAAATTCATCACTTGGTGGCACAACTTCATGGCTTTTAGCTTCATCAATTTTTTTAGTTAATGAATTGATTGCATCTTCAATATTCTTTGTTTTTGATTTAATGTCTGAAATATTTGATGATTCAGTAACATTTGTAGCATTTAATACTTTTTTTGCTTCAGAATTATCAACATTTTTTGCCTTTGCAGAATCAATTAATGTTTCCAATTCTTGCTTCTTGGCTTCAAAATTTTGTTTTACTTGATGTTTGGTTGTATTTATTTTATTTTCTAATTGTTCTTTTGCATTTTTAAGATCGTTTGAAGCATCTTGTATTTTTGAAATAGTTCAATCTTGGGCAATGTTTGCTTCATTCAAGGTTTTGGTTATATCATCAATGTTTCCTACTTCGTTTGCATCAGGGGTTGCAATTAATTCTTGTAGTTCTTTTTTTATTTTTTCAAAATCTTTTTTTGCTTGTTCAAATTCTTGTTTATTTTCTTGTTCATTAATTTTTTTAGTCAATGAATTGATTGCTTCTTCAATAATCTTTGTTTTTGATTTAATGTCTGAAATATTTGAAGATTCAGTAACATTTGTAGTATCAAATACATTTTGTGCTTCAGAATTATCAACATTTTTTGCCCTTGCAGATTCAATTAATGCTTTTAATTCTTGTTTCTTAGTTTCAAAATCTTGTTTTGCTTGTTCAAATTCTTGTTCATTAATTTTTTTATTCAATGATTCAATTGCTTTTGAAATTTCATTAGTCTTATTTTTAATGTCTTTAACTAAATCGTTATCTTTAACTTCAATATTTTTATATTTTTCTTCTTCGTCTTGTTTATTAATCTTATTGTCTATTGAATCTAAAAGTGCTCTCAATTCTTGTTTTTTAATATCAAACTCATTTTTTGCTTTATTTTTGGTATCTTCTATTAAATTTTGAAGATCTTGTTTGGCATTATTAATTTTGTTTGTTTTTTCTTTAACTTCACTAATTGAAGAATTTTCTTCAATGTTTGTTGAATCTAAAATTTCTTTTGCTTTTGAAGATTCAATTCCTTGGTCAGCGCTAAAATCAATAAGATTTTGTAATTCTTTCTTAGCCTTTGTTAATTGTTCAATAGCAATGTTAAGTTCCTTATTTTCGTCTTTCGAAGGCGAATTTTGTTTCTTTTGTTCGTTAATTTTTTTCGTTAAAACATTGATTTTTTCTTCAATGTTTTTAATAATTTCATTATCTCCTTGACTATTATCCCCTTTAAATTCTTTTTCTTGGTTAACTAGTCATTCATTAAGTTCTTGTTGTTCTTCATCACTTAGTTTTGAAATTAAATTTTGAAGTTGTGATTTTAGAGCATTAAACTTTTCTTTAACTTTTACAGTTACGGTACATTTAGATGCTAAAACAGGCACAGAGCCCGCTGCTACACACAACGCTAAAATTTGTAATGATAAATTTTTTTTCTTCATTTTAGTTATACCTTTCGTATTTATTTAAGCTTAGATATATAAAATTATATATATACATATATATAAAAATATAAAAAACAATAAAATTATAAAAATATTTTTAAACATTGCAAAAAAATATTTTTATCCATATTAAAGTTAAAATCAATATTTTTCGCTAATTTTTATTCTTATATTGAGTTTTAATATGATTATGAGATAATTTTAAAGTAGTTAAGAGGAAAGGGAATATATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAATGATATTTGTAAAGAAGTAATTAAACAAGAATTTTTAGAAAAAAATTCAAATAACAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTATTGCCGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATAGGTTTTTGCTTTTTTTCATTTTTTGCTAATAATAAGAAAATAATTAAATCATTTGCAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCTTTAGACAAAGCAAGACAAGCATACATTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAGACAAAATGGGGATAAAAAAAATATCACAATACGATGCTGAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGAAATTTTAATAGCATAAGTCAATATAAATTAAGAAATTCTTATATTTATGACATTTTTTATACTTGCAAATGAATGAAAAATGTTGTTACTAAGGCTTCAATTACCGTTACTGAAGAAGATGCTAATGGTAATACTTATTGAGAAACATATTGAGCATATCATACCGAACCTACACCTGTTGTAGAATATTTTAGAAGTTTAACCATACCTTCAAATTACAAGCAAAAACTATACTTTGAAATTATAGAAAATAATCGAAGTGAAAAAGAAGTAAAAAGACTTTTAAAAAATGAAGGCTTCCACCTTGAAAATAAAGAAGTCTATCAAAATTTTGATTTTGCATTTTCTGATGACCAAAAAATTGATTTTTTAACTTATTTCGATGTTAGAACTCAACAAAACCTAATCAATTATGCCCAATATATGAATCAAGAAAACATGCCGGTGTTTTCGATGATAAAAAATGGCAATTGGCTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAGGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAGTTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATGTCCAAAATTACCCTCTAAACATTTACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTCATAATGACCCAGAATCTTTGATAATATCAGCTACATTTGATTTGATTTCATATACAGCCAGAGACGCAATTGCAATTGAATATACACATGGTTACGCCGTTGAAGTACCATATGTGGCATATGATGAATTTAGAGAACATAAAGCAATAATTGTTGTTCCTTATAATATAAAAACAGAAAATGCCAAAATATTTGCAAATAGTCTTATCGCTAATAATATTTATGTAAGCGACGATCGCATAGATGAAGAATATAGCAAAATTATCCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAAGCTTATAGATGAATTATTTAGTCAGATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAATTAAAAAGCTTAATAATTTTTATTAAAATTATATTAATCTCTATTTTTGTAATTAAAATAAAAATAGATTACATTATAATTTAATTATTAATAATAAGCAATAAAGGAGCAATATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAACCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATCTTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATCGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATCAGAGGTGCTTTGAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACCGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGAACAAATTGTGTTGCTCAAAAATTAGATTTGTACAATGTGGCTATTTTTCAAGCATCCGAAATAGAAAGAGCAGATGTAGATACAAGCGAACTTAGAAACTAAATGAGCCGAAAGGCTTATTTTTTTGCTTTATTTTGCTATAATATAATATATTAATTAATTAATATATTACAACATGCGAGGAAACATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGCTCAATAGGGTTCAAGGGTTTGCACCACTTGCTATGAGAAATAGTGGATAACTCAGTCGATGAAGCAATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAATAATGTAATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACTGGAATTCACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGTGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGATTACATGGTGTTGGTGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGAGTTAAAAGAGATGGCAAGTTACACTACCAACAATTTAGAAATGGTGGAATTCCTGTTAAACCTTTGGAAGTAATTGGAAATGTTTCTGAAGTTGAAACAGGAACAACAATTAAATTTCACCCTGACTATACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAGGGAGTTTTTAACGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATCGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACATACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATTGCCACAAATAAAATTCTTTCAGACTCACTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCAGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCAGCCGGAGGTTCTGCTAAAATGGGAAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGCACAGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCACACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCACTATATAAAATAACTAGCGGAAAAAATGTTGAATATGCATATAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGAGAAACAACAATGGATCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTATTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAATATGCTAAAGCCTCTAACTAATCAAGGTTTCACAAACAAAGTATTTTACGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCGTTCTTATTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGAATTAATGGAATCACATTAAACGAAAGTCTTTTGACAGATGACATTTTAAAAACTATTGGAAAAAATTTAATCACTTTGCATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAAAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATTGACAATTCCGCCCCTGTCCATAATGACCTTTGACTATTCAATATGATAAAAGTGAATGATAAAATTTATTTTACAGATTGAGAGTATGCCACAATGGGCGATGTTCATTTTGACCTTGCATATTTTATTGAATCAAGCAATCTAGATAAAAAACAAGAAAAAGTGTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTAGTAAATGCATTAATTGTGCTATGAATAAATGCCCATGAAATACCTCCATTTGATGATAGTCTATATCTAAATAGAGTTGAAAAATACATGGAGCAATTAGAAAAAGAAAAAGAGTAATATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGATGATTTACATAGTAGCACATATATTGGAAAACTAGATGATGTTTGAGTTCAAATAAGAATTCCTTCTGACAGTAAAATAAATTATGATAATGAAACAAAACTAGTTGAAAAGTTCAAAGATTATTTTTATTACAAAGATGGATATATTATAAAAAAATGATTCCCCGGAGTAGACTTGTTTAAAGTTAAAATTGATAGTGGAATTAAAAAAGCAATATTTAACTGCGTCAAAAATTTTCAAAACCTTAATGTAGATAAGATAGAAAAATTTGATTGATTCAAATATCCTATTCAAGATGCTGAATATAAAGCATTAGTTAAAAAATATTCAAAAGAACCTTTAGTACTAAGCCATAATAACCTAAAACGTCAAAATATTCTAGTTAATAAATACGGCTTCATTAAACTAGTAGATTTCGAATATGTTGCTTTAAATAATAAATATGTAGATCCGGTAAGTCTTTATTTATTTTTGGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTGTATTTGATGATTTTATTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATTCCAAATCGCTAAGCCCTTTTGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCTCTTTGTGTTTACGAGGATGAAGATAGAACAATATGAAAATGAATCAATTCTAAACAATTAAGCTCATTCAACCACCATCAAATTAAAGTTCTTGCAAAAGCAATGAGAACACTTCATGATTCTGATGTAGAATTTCCTGAATACATTTTATCAAAGAAAATTAATTGATATTTAGATCATATGGAAATAAAAACCTTATTAGAAGATTTAAAAGGCAATAAAAGAATCAATGAAATTATTAAATGAATCAAACAAATTAAACCAGATGCAAATTGTCATAATAATTTAAATTTCAATAACATATTCTTTAATAGTAGCGACAATTTATATATTATTGATTGATCAGTTGCATATAGAAACAATCGTTATTTAGACATAGCTTTCTTATTTGAAAATACTCAAATGACACCTGAATTAGAATCTTTATTTTGAAAATCTTATGGTATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTCACAGCATATTTATATAACAAATTATTAAACACTGATTTTAACGCCGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAAGGACTAATAAATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGATGTCGACAAGCAAGAAAAAAAATCAGTTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAGCGAACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAATAAAACTTATAAAGTAATTACCCTAACATCTAAAAGTCTTAATGTTTCTGATATTATGAGCGAAAGCGCAAGATACATGAATATTCCACAAAAATCATTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAATACCAACTTGTTAAAAAATACAAAAATTAATGAGACAAAGGTTACAAAAATAACTAATAGTAACCAAACTATTTTTAAATTAGATTTAGTTCAACTATGAAATGAAATAAACTCAAAATCTAATAATAAAATATCTGAGTTTGATTTTTTAAGCAATATCGAATCAATAAATAATGCCATGACTTACTCTTATATAAAAAATAACAAACTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAGTTCTTTGAAAATTTTGATGTTGAGATTCGTAAAGAAAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATTGAATTAAACGAATCATTAACCGAAATTAAAAAAATCACTTTTGATACTAAAATTACATTAAAATATGAAATATAAAAACAGGCAAACGCCTGTTTTTATATTTATATATTCTTTGAATTATTTTATACCGTAGTGAATCTTTGTAACTTTTTCTAAAGTATCTAGAGGTAAGGCACTATTCTTTAATAGAACGTCAAAGAATTCTTTTATGTATTTGTGGTTTTCTTTTTCAACAAAGTCTCTTCTTGATAGTCCTAAGTGTTTACGTACTTGGTCGTATAGAGATAGCATCTTTTCTTTACCAGCATTGTATGAAATTGCTTGTCCAGCTAGATTTAGGTAACGTTTTGATTCAGACATAATGTCACCAACACCTAATGCTGAGTTTGCTGACATAAATCTTCTTACATCATTAATTGATGCATTTGCAGGTAAATCAGATTTTGCTTCAATTCCTTGTCCATGGTATGCAGGGTCAACTGCTCTACGCATATTACGTAATTGTGCTTCGTTCAATGCTCCAAAGTATTGTAACATGTTTGTTAATTTTACTGCTCTTTGTGCATGTTCTTTATCGTCTTTTTCACCTTTTTTATCAGTTAATGTTCAATATACTCCACCATGTAATTCTTTGATTTGTTTTAGCATGTCGTCTGTAATTTCATCTTTTGTTTTTGCTGTAAAGAAGCTTGTTATTCCTTTTGCATGGCTAAAGTCAGTTGGTTTTGCATAGTAATCGTTATCTTTGTTATCGTAGTCAGGAGTTCCGTAGTATCCTGCTTCAATTCCAAATCATTCCATAAATAATGCTCAACCTTCAACGTATGAAGTAAAGTCAAATGCATTTGCACCTAGATTTTCGCCGTTTTGACTTGCTAAGTGTTGACGTGCGTAGTAAATTTGGTTGTGGTGTCCCATAATTCCTTCGTGGTTAGCGAATGAAGTAACTGATCATTTTGGCAATGAGTAGTATGGGTCACAGTTGAAGTTAAATGCACCTTGAACAGAAGTTACATTTCCGTTTGCATCCAAAATACGTGTATCTGAGTTGTATGCTCCAACACCTTCTTGATCACGTCTTTCATATAGATATGTTACTGCTTTGTAGTCAGGAACAGTTTTACCGAAGAATGATTTTCCGTTCTTGATGGTTGACTTCATGAATTGGTCATAAGCCTTAAATGCTTCTAAAGCTCCATAGTAGAATTGTTCGTTTGTGATCGAACCATATTCCTTATTTTTGTTTTCATCTCTAGTTAAGATGTCATATCCGTAAGCACTTAAATTGGTCTTGTATTCTTTAAGAGTTGGATCTTCTGATAATGACTTCTTGTAATCTTCAGTGTAGAAAGTTTTATCTTCACGACCAAAGAAGAATTGTTCTTGGTTTAATCATTTTGCAAAGGCTTGTAAATCGATGTTACCTTCTGCATCACGAATTGTTAATTTAAGATCTTTAACTGGTTCTGGTCCAATACCATCAGCGTCATATTTGATTGTGGTTTCTCATGCTCCATTTTCGCCTGCTATTAATTTAGCAACTTCTGATGCAATAGCAGTCATGTTCTTTGAAACACCTTTTGATGTTTTGTATCCTGATTGATATACATCGTTTGGAGTGTCTTCTGATGTGGTTGCCATTTTTGTGATTTGGTCATAAATGGTCTTTCCACTTTCAGTTTCGTCGCCACCAACTTGTGAGAATCCAACACCCTTAGCTTCTAAGTCAAATTTGGTAAAACCTAAACCATAAACTGATCTTTCAATAAGTTTTCCGTCTTCTTGTTTTGAAACTGATATTGTATTTTCTGTTTCTTTGTAAACATTTTCTTTATACAATTTAAGTTCTTTTAGATTTTGACCTAAACCATGTTTTGAAGCGTTATAGTATTCTGTAGCATAGTATTTTCAGAATTCTCTTAGATCTTTCTTGTCTGTATCTGTGTTTAAATCAATTAAATCTGGAACACTAATTTCTGTTAAAGTGTCGTCGTCTAAGAATCTATTTAATTCATCTGCAAAAAATTCTCTAAGTGCTTCTTTAACATTGTTTTTGATAACAACTTTTGATAAAACTATATCTTTTTTAACAGCTTCTTGTAATCTTGCTTCTGCTTTTGCTCAGTCTTCTTGATCTAAAATTGTTCCATAGAATGAGTTTGCAGCATAAACATTTCCTGCTCCTCATCCATATTTAGATAGAACATTACGTCCACCTGCTAGATAGTTCTTTTGAACCATTTGTCAGCTGTAAATTGTTGCATCTTTTCATGCTGTTGTAATAGCATTTAAGTTTTCTTCTTTGATTAATTCTAGCAAGTTAATGTATTTTTCATAGAAGTTAGCAATTGCTTCAACTTGTTCTCTAGTTGGTTTTTCAAAATCAACATCTTTTAATTCTTCAACATCAGCAGGATTGTTTAATGCTGAGTCTAGTTGGTTTCTAACTTCAACGATATAATCAGCTACAATGTCATATATTTTTTGATCTTTCTTTGCTGATAAGCTTGAAACTACAACCTTAGAAAGTTCAAATTTTTCTGTAACTTTATCGTTTATAAAGGCATTAGTTTCTAGAATTTTACGTTCTAAAACTTTTATTTCTTTGCTAATAGCTTCCTTTTTATCTTCAGCAGCAGAAGTTAATTCATTTTCCTTAGCTGTTTTTTTAGCTTTTAAGTCTTCTAAATCTTTTTGATAAGCTTTAATTTGGTTTTCAACTGACTTGATGTTTAAAACACTTCTTCTTACTTCTTTATTGTGGACACCAGTTGAAATTCCTCATCCAATTAAAGGAATTCCGGCAGTTACAACTGCGGCTCCAAGAACAGCAGCGGCCACAAAAATTTTAGATTTTTTTGTCACTTTTTTATTTCCTTTCACTTAATATAAATGTATATACGTATATATTTATATTTGAGTTTTTTTATATACATATATATCTTAAAAAATTGTTGGTTATTTTAAACCTATAGAAAATTATAGAATGTATTAAATCAATGTTTAAATATATAATGTAATAATAAAATTTAGTAAAAAAAAATAACCATAAATACTTTTAAAAATGAAAAATAAAAAACAGAAGATTTTCTGCTTTTTGGTTTACTTGGTATGGCGGATAGTAAGGGATTTGAACCCTTGAATGTTAAAAACATTATTAGTTTTCGAGACTAACCTCTTCAGCCTCTCGAGCAACTATCCGTTAGTTTAATATTTAGGTTTTTATTTTTTTAACTAAATTAATCTAATTATTAAAACAAAACAATTATAATAAAAATGAGTTAATATACGCAAAAATAGTAAATGTTTATTTTATTAACTTATCAAGTATATAAAATAAATTCAAATAATATTGTGAAAGTAAAATTTTATATATTTATGGTTTAACTTATTATTTATAAGTTTGGCTTAATCAACTTGCAATATTCATTATTGCAAATAATAATGTAAAAGAGGAAAAATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGTCTAAGAAAAGAAAGGCAAGGAAATGCTTATTTACAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATACGTTGGTTTGCCAGAGGGGCAAATTGGCAATAGCGAAGTAGGACATTTAAATATTGGCGCAGGTAGAGTTGTATATACGGGATTATCTTTAATTAATAAGGCAATTCAAAATGATACTTTTAAGGATAATGAAATTTTAAATGATGTCATAGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATCCTGAAATTAGCGCATAGTAAAGGATTAAAAAACGTAAGTGTTCATGTGTTTGGTGATGGAAGAGATGTAAAACCACAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGGTTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAAGCATATGATGCTATTTTAGGGCAATCAAAAAATGTTATTAAAAACATTGATGACTACATTGAAAGTCAATATAAAAAAGGAATATTTGACGAATTTTTTGAGCCTGCTCAATTAAAAGACGGCGCATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGATAGAGCAAGACAACTAAGCCATATGTTTATTGGATCAAGCTTATACACATACAAACCAAAAAACCAAGTACAAATTGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAAATAGCCTTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAGTAATGACATTAAACAATTAAGACTAGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGGGGAGTTGACATCGAATATAAAAATGAAAATAGAATTCTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCTAAAGAAATTACAGATTCACTATTGAACAACGTTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGTCATACCGGTAATTTGAACGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTTCAAAGAATATTAGAATATGTATCATTAAATCATGAAAATGTAACTTGATTTATTACAGCAGATCACGGCAATGCTGAAATTACAGAAGATGAAAATAATAAACCAGCAACAAAACATACAACAAGCCCAGTAATGTTTATTTGCACAGATAAAAATGTTAATTTAGAAAATGGTAGTCTTTGTGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAACAAACCAAAAGAAATGACTGGAAAATCATTACTTAAATAATAAAAAAATAGATTCATTATTCGTTTGAATCTATTTTTTTATTATTCATTTTCTTCACTAAAAGATGAATGATATAGCTTTTCATAAAAACTACCTTTAGTCAATAGTTCTTTGTGTGTACCTTGTTCAATAATTTTTCCATCTTTAACAACTAAAATTTGGCTAGCATTTACTATAGTTGAAAGTCTATGAGCAATAACAAATGAAGTTTTTCCTTTCATTAACTCTAACATTGCATCTTGAATATTCTTTTCTGTTCTTGTATCTACATTAGATGTTGCTTCGTCTAATATCAAAATATTTTTATTAGCAATTATTGCTCTTGTGATTGCAAGCATCTGTCTTTCACCTTGTGAAAAATTGCTTCCGCCTTCTTCAACTATTTCTTCGTAACCATGTTCAAGTTGTCTTATAAAATTATCGGCCTTTGTAATTTTGGCAGCATTAATGATTTCTTCATTGCTTGCACTAGGATTAGCATATCTTAGATTTTCTAAAATTGTTGTTTTAAATAGAAATGTATCTTGCAATACTATTGAAATTTGATTCCTTCAACTAGATTCATTTATTTCGCTTGATTTATAATCATCAATTTTTATTTCCCCTTCAATAGGCAAATAAAATTTGCTTAGTAAATTTATTATTGTAGTTTTGCCTGCCCCGGTAGGTCCTACAATAGCAAATACTTGACCTTTTTTTGCTTTGAATGAAATATTTTTCAATTGTAAATTATTTAAAGAATTATCATATGAAAAACTTACATTTTTAAATTCAATATCACCATTCACATTTGATATTGTAGCAAGTTTGCTTTCATCAACGTCCGGATGCATTCTAATTAAATTTAATACTCTTGTTGTTGATATTGTTGCTGTTTGAATCATTGAAATATTTTCAAAATTTTTAATTATTTGACTAGAAGTAATTTTTATATACAAAATAAATGATGTCAATGTACCGACTGTAAAATTACTATTTGAGCCAGTGCCAATTCCAGACAATGCAAATGCTCCGCCAACAAAAATTATTATTAATTGCATTATGAATGTTATTGCATTTCCATATGGATAAACTATTCCGGCATATATATTTGCTTTTAATGAAGGTTTATATAATTTTTCATTATATAAATCAAATTGTTCGTTAATAATTTGTTGACTATTCAATGAATTTATTAATTGGTGTGCTGCCAAATTTTCTTCAACAAAACCATTCAATAAACCTATATTTCTTTGAGTCTTGCCAAATAATGGAATTGCTTTTTTAATTAGAACAAAAGCCAATATCAACAAAACAGGAAATAAGGCAATATATATAAGTGTTAAATATGTAGAATAGTAAAACATAAATCCAAGCGATGTTAAAGCACTAAATATCGCTGCTAATAACGAGTTTAAGGTTCCACATATTCCATATGAAACATTATCAATATCATTTGACAATATTGACATTAATTCTCCCGAGCTAACAGTATCAAAAAATTGGATTGGCATTTTCATTATTGATTTATAAGCCTTACTCCGCATTATTTCTGAGCCATAAATTCCTGCCTTGACTGCCATTGCTGAACATATAAATGAAAAGACTTTTTCTAAAATATAGCCTAAGATCAATATGCCGATTGCAATACCAAATTCAAATAAACCATTAGAATTACTATTTCAAAACTTAAAGTTTAAATAATGGTCGATAATATAACCCAAACCATATGTGGTAGCAGTTAAACACAATGAAGAAATTAATGTAATTATTATTATTAAAAAAACATATCCACGTTTGTAATTTAAATATTTAATTAGTTCTTTAAAACTTTCTGATTTTATTGCGTGTTGTTTTTTTTGAAATGCTTTTCTTTCAAATTTTGATACTTTTTTTAAATCAATATCAACTTTTAGCGGATCAAGAATATCCCTATTCATTATTACCCCCTAACTGTGAATATGCAATTTCTTTATATAGACCATCTCTAGAAATTAATTCCTCATGATTGCCACGATCAATAATTTTTCCTTGGTTTAATACTAAAATTTCATCTGCATCAATAATTGCTGAAATTTTTTGGGCAATAATAACTAATGATATTTTTTCATATTTAAATTCTTCTTTAATATTGTTTCTTACCATAGCTTCTGTTCTAGCATCCAATGCTGAAGTAGAATCATCTAAAATCAATATTTTAGGTCGTTTGATCAAACCTTGGGCAATAGATAATCTTTGTTTTTGACCGCCTGATAAATTTGCGCCTTTTTGTTCAACAACATGTTCAAATTTTTCCTTAAACTTATTGATATATTCAAAGGCACAACCATTTTTTGCAGCTAATTCAATCTCTTCGTCAGTAGCATTGGGTTTAGCAAGCAATAAATTGCTTTTTATTGTGCCACTTAATAAGCAAGGGTTTTGATAAACGTGGGAGATATTCCTTCTCAATGAGTCTGTATCAATTTCTTGAATATTATGCCCATCAATTGTTACTAATCCTTCTTGAGTTTTAAAATCTAAATTTAATAACTTTGCAATAGTTGATTTTCCAGAGCCAGTAGGGCCAATTATGCCTAACACCTTGCCAGGCTTTAAAGTAAATGAAATATCTTCTAAAACATTAGATTTGGCAGATTCATAATATTTAAAAGATACATGGCTAAATTCAATTTCTCCATTAACAATTAGCTTATCAGATTTGATTTTTGCAATATCTGGCTTTTTGTTCATAATTTCAAAAATACGTTTTGAGGATACATTTGCCTTAAAAATATTAAATGTAGTGAATGATGAAGCAACAACTCCAAATGTAACAGTCATAACGTAATTCATAAATGTTACCCCATTGCCTATTGATTTTGAGAAATCAGCAATATTTTCCTTGCTAGTTTGCTTTCCTACAAAAAATACAATGAAAGCAATTGCTATATTTGAAATTATTTCAATAAAATTAAATGTTATAGTGAATATTATTCATGATTTGCTTGTTATGGTTCCTCAATTTTTATTAACATTTTGGAATTTATTTCATTGAATTTGTTCCATGTTATATGATTTAATAACTTTGGCGCCTAAAATATTTTCTCTACTTTCAATGTTAATTTTGTCATACATTTCTTGTTCTTTTTTTTGAATTGGATTTCAAATGACACCCGAAATTACCATAGTTAAAGTTAATAGTGGAATAACTACTAAAAATATCATTGACAATTGAAGATTTGTTAATAGGGCAAATACTAAACCTAAGACTAGATTAAACGGCCCTATAATTAGCATCCTCAAAAAAGACATTAGTCCGGTTTGAATTCTACTAATGTCTATTGTAAATCTCGTTAGGATCTTGGCATTTGAGAAAGCCTCAATATCTTTTTGAGAAAGTTCGCCAAGATGCTTTCATAAACAACTTCTTATTTGTTTTGATCCTGCTTGAGACACATATGATGCAAGCAATGAAGCACACATTGCTGAAATAGTTCCTATTATTAAAAACAATGCAAAATATCCCCCCAAATGCATTAATGCACTCTGGTGGCTTTGACATAAAAAATTAATTCTTAGAATTTCAATTGTTATTGGTCTGTTTGTTGACAAATTATTTTGAATTAAATATGCATTTTCACCAATTAACCTTGTCAATTGTCCCAATAATATTGGCAATACTAAAAATGAAACTACTTGTAGCAATGTTAAAGATATAGTTAAAAATGACAAACATTTGAATTTGTTGGCCATTAATTTATAAAGTTTAAACATTGTTTCCTCTTTCTTTTTAAATTTGTTTTAATTATTTAATTTAAAAAGTTACCTTATATTATCATCAAAAAATTGTTTAACTTCTTTTGCGATATAAAGTTCTTCATCTGCCCTTATTATATAAATAGGAATTGAAGAGTTTTTAGTTGAAATTAATTTGTATGAATCAAAATCTCTTCCTTTATTTAATTCACTATCAATTTCTAATTTAACTAAATGAATTTTATCAACAACTGCTTGGCGAATATATTCATCATTTTCACCCACACCCGCCGTAAAAACAATAGCATCAATTTTTCCTTCAATCTTATTCATGTAATTAATTAAATAATCTGCTATTTTTCTTACATATAAATCTATTGCAAATTTTGCTCTTTCATTTGTTTTTTTAGCTTCTTCAACGTCACGAATATCAGAAGAAATTTTTGAAACTCCTAATAATCCTGATTCTTCATTTAACATTTTTATTGTTCTTTCAATATCTCAATTTTTATGTTTCATAATATATGGAATAATTGAACAATCAACATCACCAGAACGAGTGCCCATCATAACTCCCGCTAGTGGTGTAAATCCCATTGAGGTATCAATTGATTTACTATCTTGAATGGCTGCTAAACTTGCGCCATTTCCGATGTGCAAATTAACAAAATTAACTTTGTCTTTATTTAAAATTTCTTGCAATTTTAATGTAATAAAGTGGTGATTCAAGCCATGGAAACCGTATTTTCTAACATTTAATTCATCGGTTATATTTGCATCAATTGCATAAGTATATGCTTCTTTTGGAAGGGTTTTATGGAATGAAGTATCAAAGTGGAATGTCATTTTAGCAAATGGTAAAATTTCTTTAAAGGCTTTTACAGCTTCAAGAGCACCAGGATTATGAACTGGTGCTAATTCAATAGCATCTTCTAAATATGCAATAGTTTGAGGGGTAACTTCTGAAGTGTCTTGTAGATGTATTCCACCATTAACAATTCTAAAAGCAACATATTTAATTTCATTAAAATCTGAAATAATATTCTTATTAATTCATAAATTAACCAATTGTTTAACTGTTTCTAAAAAACTAGGTAATTCAATACTTACTTCTTCTTTTTTGTTATTGTAATTTAAAATAATAGAACTATTTTTAATTTTAATTCTTTGTGCAATTCCTTTGGCAATAATATTTAGATCTTTTGAATATAAGGTTCATTTAATTGAACTTGAACCTGCATTAATAATTAAAATTTTTGACATAATAGCTCCTTTATAATTTATAAAATTATATTAAAAAAAATATCTTTTAAACTTAATATGATTAAAAGATATTAAAAAAGATATAAAAAAAAGCAACGTCCTATTTTCCCATTGAGGTATCGTCGGCACTGAAGGGCTTAACTGCTGAGTTCGGAATGGTTTCAGGTGGACCCCTTCGTTATGGTTGCTACGTGTATTATTATATACTATTTTTAAATATGTAATAAAAAATTTTTATTTTTTTATTACTTTTTAAACACTCTTATTTTTGCAAGACTATTACCAATATTTTTTCAATAATATCCTTCTTTGTCTAATAAGTCTTCTGTTATTTGGCGAATAATCCCACTACCAATGCCTACAATGATATCAAAGTAATTATTATATTCATCTTGTTTAAATTCATACAAAGCGTTTAAAATTGCTGCTGTTGCAGAGTTTACATCATAACCATGAAGATCTAACACCATTGGAAAAAAATTGAACTTCATTTAATATACCTTCTGACAATTAGGGCAAACATATGTACCACGTTGGTTAACTTTTAATTTAAGTGTTTTTTGACCACAAACCTTACATTTTTTTATAGTATCACTATGAATATTTAAAAAGTCTTGATATTTTCCTTCTTGTTTATTTAATGATTCATAAGAAAATAACGTTGTTCCACCTAATTCATAACTTTTTTCCATTATTTCATTTGCATTTTTTATTATGTCATTAAGTTGATCTAGTGATAAATCAGAAGCTTTTGAAAGAGGATGAATTTTGGAAGCAAACAATGCTTCGTCAGCGTAAATATTTCCTATACCAGCAACAAAACTTTGATCAAGTAATTTTGTTTTAATGGGCATTTTTGAATTTTTTATTTTTTTAAATAATGCTTCAATGTCAATATCCTTTGGCTCAGGTCCAAGCAAAGAAATTGGAGGAATTTTGTTATAATTTTCTAAGTTTCTTAGCATATATGTGCCAAATAAACGTTTATCTAAAAAGTGCAATTCGCTGTTGTCGCTAAAAACAAATCTAGCAACTAAGTGCTTATCAATTTCTTTTGGAGGGTTTAAATAATAACGATATTTTCCCTCCATTCTTAAATGAGAAAGTAAAACTAATTTTTCAGATAAAAAGAAAATTATATGTTTGCCAATGTTATCTATTTTTTCAATTTTTTTATCTCTTAAAATTGAAATGAAATATTGAGGGCTAAACTCTTTAAAAATTTTGGGTTTGTATATAATCAAATTTGTTATTTTTTTATTTAATATAGTATTATTAAGGGCTTTAACAACAACCCTAACTTCAGGTAATTCAGGCATAGTAAAATTATAATACTTTCGAATGACATTACTCATATGATTAACTGCAAAAATAAAAGTGTTATATAATTTAAATTATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGTGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGAAATTCGCAAGCAGATGAATTTGCTAAAAAATATTGGCCTGGTGCAACTACCATTGTCGTAAATAATCAAGGTTTTAGAATGCCAAACCAAAAAAAATTGCTTGAATATATAGAAGTAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAATGCACCTGTATGCAAAACTATTGAAAATGCAAAGCAATTATTCCCCGAAGTTACTAATGTATATAATTTTGGTCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAATATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGACGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCAGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGCGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAGTTGAAAAAATTTTTCATAGCTGAGAAAACACCGAATTTGAAGGTGGCAGACATCTTGAAAGAATTAAAAAACTAAGCTCAATTGGTGAAAACTAATAAATTTATAACAAAAAATAGGCTCATTTTATTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTAGAAGTATTAAATCTTCCAGATACATTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCAGCAGCTAAAATTTCTTTCTTAATCATATTTTGTTCCAAGAATAAACAATATGTTTCAATTTTAACCGTTTCTGTATTGGTATAACCTCCCAACATACGATCAATAGTGTAAGCGTGTCAATAGTTAATTTCTTTGAATTTGTTTAAAATTAAATCAGGATTCTTGCTGTAAATTTCTAGTTTTACTTTTCTGTATTTTGGATAAATTGATGCCATAATAAATGTATATAAGAATATATATACAAATGTACAAATAATTCTTAATAATAATGCTGCATGGTTTATTGGATTTCCATTTTGAGCATGTGATATTACCCCAGTAAGTTCGCAAATTGAAATAACAACTATTGAGAATACTGCGAAGCAAATTGCGACTATTAAAGAAATTAAGCCTGGTTCTTTTTGCTTAGTTCTTGAAATGTAGTAGACAACAAAGTCTCCCCCTGCTGTTGAAGCAGAATTTTTTCAAGCAATTCCCCCTGCACATCCTCCACATAATGCTCCAAGCACTGCAAAAATGATAATTGGTCAAGTAGGATTGTTATAACCATGTATTAAATGTTGATAGAAATATCAATAAGACATTGTTTCTTGGTAGCTTAGTTGCACGCCGTTTAGAAGTATTCCATTATTTCAAGAAGGATTAAATCCAGTACTTTTTATAGTATTTCAAACGTTTGAATAATGTGTTAGATAGTTTCCATAGACATCCCATGGTATTAATACATTTAGAGAGTTAGCAACTTCGCCATGTGCTGGAGCTAATGTTGCAGAATGAGGAGCATACCAGTTTACATAATAAAACGTTATTTGACTAAAGAAGTGGCCAACTTGTGGAATTAACAATATTGATTGAAAGATAACTTGTCAAAGTAACCAATAAGTTGTTAATATCATAAATAGTCTAGAATTTTTCTTTCAAAATGCAATTACAAAAGGTAAATTCATTGCTATATAAATATAAGCAAAATATGGTGCGGTTTGAGTTAATGTTAGAGTAATGGCTTGAACCAATGCAGATAACCCAGTTGCAACAGTGGCAGCCTTAGCCAAGAATGTTGCAACACCAATATTGTATAAAACGGCCGAAATAATAATCATGACCATTTTTTTAGGAAATTTCTTTCAAATATTAAAGAAATTTACCCCATAAGGATTTATGTCAATTTTGCCGACTGATCCCTTTTCTTTTTTGGTTTCTTTCTTTAAACGTTTACGTTCTTTAATCTCAGCAAGTTCCTCAGCTGTATGTTTCATTTTCACCTCCTTTTTATTATCGTGTATATTCTAACACTTTTTAAGTGTTTATATTTTTTATTAATTATTTTTTTTAATAAAAAATACTTTTATATGACTTTAATAATTAATCTACGTATGAGGTATCAAATGAACCAATTAATCCATGAATATTTGAGATACTTATTCAGGCTTTTTTATCAACGCTTCTAACTTCTTCTAAAATTGATTCTTTTTCTAAATATAGGGCAATTGTTTCAATTTTTCCAAAATCTTGATGGTTATATCCACCAAATCCGTTGAATACATTGAATGAGTGAACATATCCAATTTGTTTAAATCTTTCAGCAACTAGTTCTGGTTTTGTGGTGTAAATATCAATTCTTACTTTCTTGTATTTTGGATAGAACTTATTTACTAACATTGAAAATACTAATAAGTAAATTGCAGTCCCCATAATTCTTACTAAAAATTCTGAAAAATCTCATGTATGTCCAATAATGTTTCTTGTATATTCTAATGAACCTATTACAATTGTTGAACATGATGAAAAGCAAATTGCAACCATTAAAAATACTGTTCCTACTGGTTTTTTATTTTTATATGCAATGTAATTAGAAATAACAGATGTACCGCCAATTGAACCACGTTGTTTTCAGGCAACTATTGATGCAAGGCCTTCCATTATTCCCCCAAGAATTGCATATATCAAAATAGGTCAATTTTGGCCATTATATATTCCATAAATTTTAGTATTTTGACCGCCTACTAAATCAATACCTGTTAATAGACTAGGAATATTAGCAAGTGATAAAGAATTAGATTTTTCAAAAATTAATTTGGCATTATCAAATACCATTTTTAAATCATTAGTTTTAAATAAGTTGGCTAGATACTTAGCAATAGCCAAATCGTCTCTGGACAATGAACTGCCAGTTCAAGCTGTTTTAGTTGATATACTTAAAGCGTAGTTAAAAATATCTTGATAATTTTCTTTATTAATATAATTTAATACTCTTGTATCGAAACCATATGGATCTCAATAAAATTTGTATGTTAATCCGTTATTTGTAAAAGTTTGTCCTATTGGATCATAGAGATTAATTCTTTGATTAATAAAGTTAACAATAGGATTTGATGCTCTTCCTTGATATTCAATAAATATACATTGTACAATTAATTGGAAAAACATTCAATAATAAGTTATTACCATAAACGTTCTAGGATTTAACCGTCAATAAATAAACATTAATGGTAAGTTAACTAATACATAAAATAAACCATAAAATTGTGCGGTTGCAGGCGCTGTAAAAGTAATTATTTGAGATAAAGATGAAGTTCCTGAAGCTATTGTTGCTGCTTTTTTTAAAAATACTGTAATTGCAATATTATATAAAAATGCACTTACAAGCATTCAAAACAACTTTTTAGGATATTTTTTTCAGACATTTATTAAATTTATTTTTTGTGGATTTAGAATTTTCATTTTTTCTAAATACCGTTGGTGTTTTTGCTCTTTGGTAAGAATGATTTTATTGATTTTAGTTATTTTTTTATCTTCTAAACTCATATTACCCCTTTCTTTAGATTTATTTAAACTATTAAATTATTAATTTTTCTTTTTTTCATAAATAAACCTGTTGCATTCTGGTTGGAATAATAGTTGTACAATTCCAATAGCCCCATGTCTATTTTTTGCTACAATGACATTGGTTAAAGAGGAACTGCCGTTAACAATTTCACTTTTGTCAATGGCTTCTACTTTATCGTTATCATCGTTAATGTTGTGCTTATTATAATAATCTTTTCTATGTAAAAAAATAACCATATCGGCATCTTGTTCAATTGTTCCTGATTCTCTTAAATCTGACAAAATAGGAGTTTTATCTTCCCTTTTTTCAACATTTCTACTTAACTGGCTTAATGCTAAAACAGGACATTCTAATTCTCTAGCAAGTTGCTTTAAGGATCTCGAAATCTTGCTTACTTCCAATTGACGATTTTCCATACTTGCTTTTGAAGAATCTGACATTAGTTGTAGATAATCAACTACAACTAGATCAACTTTATTGTTTCTAGCAAATCGTTTTGCAATGGTTGTTATATCCGAAATTGTTGATGTAGGTTTATCATTTAAATACATTTTTCATTGTTTCATTTCTGAAACAGTTCAGGCTAGATTTTTAGCGTTTTCTGTTGAGATTTCATTTTTTCTCATTAAACTAATCGGAACCAAGGAATCAATTGACATCATTCTTTGAACAATTTGTGAATTTGACATTTCAAGAGAAAAAAATAGCACCGTTTTATTGTTTTTGGCAACATTATTTGCTATATTTAAAGCAAAAGCAGTTTTTCCCATAGCTGGACGAGCCGCTAAAATTATCAAATCTCCGGGATTGGCGCCTAAAATTAATTCATCCAATGGTGGAAATCCAAAAGAGAGACCAACGCCAATATCTGATTTTTCTTTGCTCATTATTCTATTAAATAGTTCATCTGCTTTATTTCCCACTTGTTCATAAGCAGTATTTATTTCTGAAATATCTAGGTTGATTAAATTCAATTGTAAATTGGATAATAAATCTGAAATTTCATAAGGCTTGTTTTCCATAATATTTATTGAACTATTTAATAAATCTCGTAAGCGATCCTTCTTGTATTGATCAATTATTTTCTTTGCATATGACTCTAATTCGGCGCTAAAGCCCGCATTTTTAGTAAGTCAGCTAATATATTCACTACCATTATAGCCAAACGAACCAATGTGATCAATTAATTTTTGGTCATTTAAATAACTCAATAAGTTGGCTATATCGAATTGTTTAGAGGCAATATGAACTTCTGAAATAGATTTAAAAAGAATTTGATTATTTTTAAAATGAAACATTTCAGGTTTTAAAATTTCAGCAATTGTTAAATAACAATTTGGTTCCCTAATTAATAAACCTAATAACGATGTTTCATTATTAATAATTGCTAATTCTCTTTCTTCTTGAATGTTCATTTTTGGCCACCTTATATTTTATTTTGATTATTTCTCTTCTATGATATTTATTTTTAGTATTGCAGTTACTTGGTCATGTAATTTAATTTTTACATCATGCAACCCAAAAGTATTATATGTTACCCCTGATTCAAATGCATGGCTTGGGATTTTAATGTTGTTTTTTATTAATTCTTTTTCAATTGCTTTTGTTGTAACGTTTCCGTGCACTATATTTCCATTAGATTTTAAATAGAATTTTAAAACTAATGATTCAATTAATTGTTTGATTTCATTAGCCTTGGCTATTTGATTTGCTAAATCTTCTGCAATATTTGATTTAACTCTATCTAAATTTGCCAATGTTTGTTTGTTAATTGGTTGAGCATAACCATTTTTAATTAAAAAGTTTTTTGCAAAACCGTCTGTAACTTCAATGATTTCATTTTTTTTATGTTTTTGGTATTCTTTAATTAATATTACTTTCATTATCTTTCACACTCACTATTGCTTGTATTATATTATCAACAAATAAATTCAAATTTTCAGTTGTTTCAGCAGCGGCTGCCCCAAAATGGCCACCGCCGTTAACTGCTTCTGCTATGATTTGAACATTTGTATTAATTCCTCTTGCTGACATTTTGTATGTTTTTGTTCCTGGTAATTTAGCAACAACAAATGCAGCTTTCCTACCATTAACTCTTAGTATTTCATCTGACGCTATTGAAATAATATCTGTTGATGCTTCAATATCTTTGTATGCCAAGAAATATCCTTTTTTAATTTCTTGCAAATTAGCTAATAATTCTTGAACAATCTTAAATACTTTTTCATCGAATTTTAATGTATCAACAGATTTATCGTTTTGTGCTCCTCATTTTTGCAATATTGATGCTGCTGCAAAAGTTTTAGCACTTGTTTGTTTTTTAAATGTATTAGTGTCTAAATAAATTCCATCTAATAAATATTGCGCTGTATCATTATTAATTTTATCTTCTTCGTGTAACAATGTGATGATTTCTGTTAATATTTCAGAAGCAGATGATGCCGAGGAATCAATATATACATTTTCTCTATATGCTATTTCATCAGTATTTTTATTTATTCTATGATGATCAATTATCATGATATTTTCTTTTTTTATGTTTTTAAAGACATTAGCATTTTCAATTCTAGTATCATCTGAAGTATCGCAAATTACAACCAACGTTTTTTCGTTGTTGATGCTTTCTGCTTCTTTAGGACTAATAAAATATTCTTGAGTTTCCTTGGACATTTTATCAATAACCCTTTGAGTAGTACTATCAAATGTCTTGTTTTGAATATAAGCATTCTTTTTATAGGCTAATGCTAATTGCAAAATTGCATATGTTGAACCCAAAGCATCAAGGTCTGCATTCTTATGGCCATAGGCTATAACATTTTCAATATCTTTTGATTTTATCTTTTCTAATAAGTTTTGTGCTATATAGTTTACATTTGTTCTTGATAGATTTACATCAATTTCCGAGCTTGAGCCATAGTGTCTAGCTTTTTCATTTTTTGTTAAAACTGTAATTTGATCGCCACCCCGCATTTGTGATTGCATTAGAGCATCTTTTGCTAATTTGACTAACGTATCGTATTTAAAAATACCATAACCAAATCCACCAGAAATTGATATTAAAACATCTTTAACAATTATTTTGTTTTTTATCGAAGTTATAACATTAAAACTATCTCGTTCTAGTTCTTCTAATGTTTCTTTATTTGTTATTAAAAAGAATCTTCCGTTTTCGTATTGTCTATAAATAACATTGTATTTTTTAGATATTTCATCTAATAAATTTACTACAGAAGCATAAATTTTAAAAATTTCTTCTTGGGATAAAAATGTTTGATATAAATTAATATTGTCAATGTCCAATTCCCCAAACACAATTCTTTGTTTTTTATAGTCATCTAATAAATTTTGTTGAGCAGTAACATCTCTTAAAATAACTAGATTTTTTTCAAATGATATATGAACTTCGTAATTGCATCCATCTATTTCATACATGAATTCTTGATTATCTGAAGTTCATTGCTTTATATTAAAAATATCTTTAATGTTTTTGTTGATTAAATTGTTATGAAATTTTATTTTTAAAGATTCTGATGCTCATATAATTTTTCCACTATTTAAAAATATCAAAATTCCCGTACCAAATTGATTGAATTCGCTTTGTAAATAAAATTCCAATGATTTTTTAGCTAATATTGAGTTTTTAAAATAACGTTGCAAAATAATAGTTAAATAAATTGCAAGGCCGATTAAAAATAAAGTGTAAATTGCCATTAACGCTATTTTTGACTGTTGAGTCTTTAAACAAAAAATGCCAATAAAGAATAATATTGTTGGCAAAATAAATAATAGGATAACTTGTAAAGCTATATAAATTTTTTGAATATCTTTGTTTTTCATTTTATATATATAAAATTATAATAGAAAGTTAACAATAGATTCAATTAATGATACTACAAGTCATAAAATTGCATTATTTCTTCAATACCTTGTTTTTTTTCGGATGATACTAAAAAAATATTTTCAGCTGTTTTTAAAGATAAGGCTTGATTTTTAACGCAATAACGTTGAGATTGATTTGCCTTGTCGATTTTTGTAACAACTATATCAAATTTTATTTTAAGATCGTTAAGATATTCTATTATTTCTAAATCACTAGCGCTAAAACCAATTTTAGCATCAATTAATAGCAACACTTTTTTATTGTTTTTATCGTTTTTAAAATAAAAGTCAATCATAAAAGAAATTTTATCTTGCAATTTCTTTGAGATTTTTGCATAACCATAGCCCGGTAAATCAATAATTATTTTATTTTTGTTACTTTCAAAGTAATTAATTAATTGTGTTCTTCCTGGATAAGATGATGTTTTTGCAATATTGCAAGAAGCCAGAGAATTAATTAATGATGATTTTCCAACATTGGATCTTCCTCAAAATACATATTGTATATTGTCAGAATTAATAAAATCATCGTGGCTCTTACAACTTTTTAAAAATTTTCACATTATCTATCTCTCCTTAATAGTTCATATGCTAACCTAGCAATCATCATTGCATTATCAGTTGTATATTTCATTTCCGGAATTATGGCATTTGAATGGACATTTAAAAACATTTCTCTAATTGCTTTATTAGCACTTACTCCGCCTGCCAAAACAATTACTCTTGGATGATATTTTTTAATTGCAATTTCTAAATGATTTTTTAAATATAAGACTACTGTATTTTCAAACATTGTTGCATATTGATCAACATTGATCTCTTGTTTTCTATTGATTTGGTTATTTATAAGATTTATAACTTGTGTTTTTAATCCACTAAATGAAAAGTTCAATTGAGCTTCAGTATGTGGAATAGTAAAATGTCCTGTGTATTTATCTCTAAATTCCATTCATTTTTTATCAATAATAGGTCCTCCAGGAAAACCCAAATTTAATTTTCTTGCTATTTTATCAAATACTTCTCCGACGGCATCATCTTGAGTTTGGCCTATAACTTCGTATTCATTTTTTGATTTATATAACATTAATTGTGAATGCCCACCTGAAACAATTAAACCTAAAGCAGGATATTCAACTTCTTTGCCTATAAAAGCAGAGAAAAAATGCCCTTCTAAATGATTCAAAGGCAATATAGGTTTTTCAAGTAACATTGCTATTGCATTAGCAACAACATATCCAACATGTAAAGAGCCAACTAATCCCGGTTCTTTTGTATATGCTACATAGTCAATCTTTTTAATATCAATTATTTTTTTTAATTTTTCAATTAAAATACCTATGTTTTTTACATGCAATCTTGATGCTAATTCTGGAACTGTGCCTCCATATTTTCTATGAATGTCTATTTGGCTAATTGTTTCCATTCAAATTGGTTTGTTATCTTCTAATAAAGCAAATGATGTATCATCATGAGAACTTTCTATGGCAAAAATTAACATTTTATCTCTCCTATTTGTGGATCATGCAAATAAATTAATTCGCAATTTTCAATATCATTTACTTGTTTAAATTCTTTTAAATATTGCTCTATATTTTTTGAAAAAAGCTCTTTATTGAATAAATCAAAATTGCCTAAGTTTTCTTGCATATGACATTCAAATGGATCAACAATTATTGAATAAACATTGTATTTATTGGCTTTTATATTTATTAAATTGCAATCTTCATGTTGCAATTTTTTTTGTGTATAAAATAAAGAATATGTTGGGGCTACAAATAATTTGATATTTGATAATTGTGCTACTGTTTTGGCAAAAACTAAGCCAATTCTTGCACCTGAAAAACTCCCAGGTCCTAACGTTGTATAAATTTCTGAAATTGAACTTAATTTAAAATTATTTTTATTTAATAAATAATTTAAATTTTCAAAAAATGCATCGGTTTTTTTAACTAAGTTAGCAATAAAAATACAATCAATCTCGCAATAATTTTCATCAATTAAGCTTATATAAAAATCGCTTAATGAAGTTTCAATGAATAATTTCACTATTGTTCCTCCATAACTTCAAATACATGGTTTTGTTCATTGTCAAAATAAACTTTTATTTTTATATAATGTTTAAATTTGGGCAATGTCACATTTTCTGCTCATTCAATAATTGCTAATTTATCTTCAAAATAATCTTCATAAGCAAATAAATTTGTAGTTAATTTATAAGCATCAATATGTACAAATTTATCATACACTAAAATCGTATTGAATGTGGGTGATATTACAGTCTTTGGTTCGTTTAATTTTTTTGCTATTTGACTTGTTAAAGTTGTTTTGCCTGCTCCGAGTTCGCCTATTAAAAGCAAGGCTTCTAAATTTTTAAATGACAAAATATAGTCAACTAGTTTATCTAATGGTTCGTTATTTTTATAAATAAATTTTAGATTTGACATGATTTCTAACCTTGATAATAAAATACATTATTATTTTACCATTTATGCACCTTTTTAAGGCTTGTTACAAATAAAAAATGTATTTAAAATTTAAAAAATAAACATTAAGTATTTAAAACACAAACTTAATGTTTATTTATTTTTTTAAGCAAGATGTTATTTTATTTATATTGAATATTTTCTCAATCAGATTGTTTAAAATCAGTTAAAAATTTTGTTAACAAATCAACAGCTGCTAGTAAATCACTTATGCAACATACTCCGATTGGTGAATGCAAATATCTTTGAGGTAATGAAATTGTTAATGTGGCTGCTCCACCTTTTGAAAATTGCAATTCAAAGGCATCAGTGCCACCACCCATGGCTACATATTTAAATGTTGGAATATTATATTTCTTAGATAAATCAACAAAAAATTGTACCAATTTTGGATCCATCATTGTTCCTCTATCTTTTAATCTTAAAGCAGCTCCTTTATATAAAGAAACATTCCCAGGAATTGTATTAGGTGTATCGTGAGATGCAGAAGTATCAACTGCTATTGCAATGTCTGGATCAATCATAGACGCAACTGTTTTAGCTCCTCTTGTTCCAACTTCTTCTTGAACTGTACCTACTAAATATAGATCAATGTCAAGATCTAATTTTGCAACAGCTTTAGCAACAAATTCTAACACGGTTACACCAGCACGGTTGTCCATTGCTTTTCCGCCAATTAAATTTTGATCATTAAATAGTATTGTTTCTCCACTCATGTAAACTCTATCGCCAACATTAACACCATGAGCGATAGCATCTTCATCGCTTTTGAAACCAAAATCAGCAAATAATTCATTTTCTGTAATTGCTTTCTTTCTTTCTTCTTCTTGCATTATATGAATACTTGTATGTCCAAATAATCCAATAAATGTTTCGTTATTTGAAGTAATTAATTTTGCTTTAGTTCCAATAACTGCACATGGTCATACACCACCAACTGTTGATAAATATAATTGTCCTTTTTTATCAAAATTTCTTACTACATATCCAACTTCATCCATGTGTGCTGCTAATAAAACCTTAGGAGCATTTGGATTTTTAGATGGCTTGTGCATAATTAAACAGCCAAAATTATCTCTGCTAAATTCAAATCCTTCATTAGCTAATTCTTTTTTTAATTCATTAACAACTGGTTCTTCGTAACGAGACATTGCTTCAATTTCCATATATTTTATTAATCTTTCTTTGAATTGATTTTCTCTTGTCATATTAATTCCTCCTTTATTTAATGTAAATGTATAAGATATATTTTATAACAAATTGTATAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTAAATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATAACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCGTCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGTAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTGCAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGTTTCAAAACAACCTTTAAATATTATATACACTTTTTTTTATTTTTTCAAAAAAAATTAAATTTTTTAGCGATATAAAATTGCTGTTTTAAAACAGAAAAATAAATATGTATATATTATAACTGTTTTATTTTTTAAATAATAAAAAAATCAGGTTTTTTTCCTGATTCTAATATCTATTCATTATTTTATTGTTAACCTACTATTTCTAGATCTGCATAATCAAATTCAGTTGGAACTTTTCTACCAAATTGTTCAATATTAACAAAGGCTTTTTGGGTAATATCATTATTTTTAATTATTTCGCCAACTTCATCTTTGAATATTCCACTAGAAATTTTTACTAAAGCCCCTTCTTTAAAAGCAGTTTCAATGTCCCCTGCTTTAAATTTAGCTAGCAACATTGCTTCTTTATCAATCATTAATTGGAATTTTTTATCACTTATTGGGGTTGGTTTTGTTTTGTTACCACTTGAACCAATTAGCCCGGTTACATATTGAGTATTTCTAACAAGATATCATGTATCATCAGTCATTGCCATATTTAAGAAAATATAACCTTTGTACATATTTACAAATTTGACAGTAAATTCTTCGTTTCTTTGCTTCTTTTCTAATTCTTTATTTGATAAATGGGGTTCTTTAAACATCCTTATATCTTTAAAGTATTCATCCATTCTTTGAGCAGCAATTTTATTCTTTAATGATTCTATAACATTTTCTTCTTTGCCTGAAACTGTTGAAATCATATATCATTTAAAATCATTATTTGTTAATTCCATATTCTTTCCTTACTAAGCCTTTATTCCTACTGAATTTCATAAACTACTAAATCCAAGGGTTATTGCAAAACAAAATGCAGCCATTAAAACTAGAAATAAAATTGTAATACCAAATCATTTTCAAGATTTTGCAGATTTTGGCCAGATAACTCTCTTTATTTCCTTTATTCAATTTCGAAATGTATATAGCTTAACTTGGCCTTTTTCTGCTTTTGCTTGCAATTTAGCTTGTTTTTTTTGTTCTCTAGTCATTATTTTTCCTCTTTGTGCAATGTTTGTTGATTACAAAATTTGCAAAATTTGTTAATCTCTAGTCTCTTTTCATTGCTTTTATTTACCGAGTAATTTTTATGTAAACATACTTGGCAGGCTAACGTAACCTTCTTTGACTTTGTATTCATAATTAGATATTATAATATATTAATTTCAAATTTTCAATCATTTTTAATAGTAATTTATATTAAATAATTGTTGCGTAAGAAATTTTATATAATTTTAAAATATGAGTAAATGAGCAATTTTGTTTTTAGTAATCTTAATTATGCTAATTGCAATGTATTTAACATTTATTTTTTATTATTTTATAAAAGTATATCCCCAAGTTTTAAAATCAAAATTGGATAATTTTATTAATAAAATTTGAACATTTATTAATAAAGCAAAAAATTCTTATGACAAAATTTGCCAATTAAGTTTGAACAATGATGAATGTAAGGAACTAGAAAAACAAATTGGAATTAATTTGCATGAAGTTTGTAGTTTATACAAGGCATGTAAAATATTAAAAACCAAAGTAGACGATATTGTTTTTTTGCATATGAACAAGCATGCTTTTTGGCATTTAAAATGCACAATTCTTATTAAAAAGAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAGCCATCTTTAAAAAAGCGGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATAATAAATAAATACAAAGTTAGTTTAAATGATTTGTCAAATATAATGGCAGGTGAACATGAACATTTTCCTCAACACTTTATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAACGATGAATATCTAAAATGAAACGAAAAAATATTAAAACAAAATTTATTAGAAATTAAAAATTTAATCATTGAATATGCAACCTTTTATTTTAAATTCAAAAACAATTACAAGAATTTAAATTATATTGCTGAAAAATACAAAAAAAATCAACAGCTTATTAAAGAATTAAATAATATTAAACTAGAACAATTAATAAAAGAAATGTTGGAAAAGAATTTAAATTTAATTATGAGATAGAAATTGAAAAAATAAAAACTTTGATTAACAAATGTCAAAATTCAAAAGACATTTCAAATATCGAACAAATTATTAATAAACTATTGGCTTTGATAAATACAACTAGTATAAAAATCTATAACTATATTAAAACTTTCAACTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGACGTTCAAAATAAAAAAATTTCTGATTTTTTTGCAAGTGAAACTAAAAAGAATTTATTAAAAAGTGAATACCTTAATACTTATAAGCCAATATTGAATAGTTTTATAGATATTAAGAATAATAATTGAATGGATATAGTTGAATTTATAGAGTTGAAGAATTGGTTAGAATCTTTATTGAATTTGCTAAGGATTCAAAATAAAATAATAAATAAAATAGATGAACAAGAATATCGAAACAATCAGATTGAATTCTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAATATTAAATATTAGTTTAAGTTCAGAAGACATTGAAAACATAAATAACTTATTTGCTTTTATTTATAAATTCAAAAATAATAATTTTTCTGCAAACGAATTGAATGACTTTTTAAGTTATTTATCTAATGAAATAAATTTTTTAAATTGCAAAATTCAATATTTTTTAATAATTAAAAATGTAGAATTTTCTTTAAGAAAAGATGTAAGTGAAAATTCAAATGCCCAAAATTTATTTAATGAGTTGCAAAAACTTAAAGGTTACAATGATGATAAAAAAGCAATTGATTTAATATATGATTATATAGAAGAACAATACAACTAATGGAGTAAACATGGACTACAATAAAATTTTAATTAGATATGGTGAATTGACTCTTAAAGGCGAAAATAAAAAAGATTTTATTAGGCAATTGCATAACAACCTATCTTGATATATAGAAAACTCAGAAATTAAAACCGAATATGACCGAGCATTTGTTGATTATTCGCAAAAAAATTTAGACTGCTTAAAATATATTTTTGGAATTTCTTCATATTCGCCTGTTTATGAAACAGAAACCAGCCTAATTAATATTGAAAATATAATAAAAGAAATAATTAGTAAAAAACAATTTAATAGTTTTGCAATAAATGCACGAAGACACAACAAGAATTTTTCAATGACTTCAAGTGAACTAAATATGTATTTTGGAGGGGTTGTTTTAAAACAAATGCCAGACAAGGAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGATGGCTTAGGTGGTATGCCGGTATTGAGTTCTGGAAAATGCATACATCTAATTAGTGGAGGCATTGACTCGCCTGTAGCAGCGAACTTATTACAAAAAAGAGGGCTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACAGATGAAAAAACAATTGCAAAAATAGATGAAATTGTCAATATATTAAAAAAATACCAAGGTAATGCTATGTTATATCAAATTAACTATAGCAAAGTAATGGAATATATTGGCCTTATAAGTAATCAAAAATACAAAATAACTTTAATGAGAAGATCATTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGTGAAAATTTAGCGCAAGTTGCCTCTCAAACCCTTGAATCTATTTATACTATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCCTTAATTTCTTTTGACAAAAATGAAACTATTGCTATTGCAAAAAAAATTAACACATTAGATGTTTCAATAGTAAGGGCATGTGAAACATGTGAATTATTTGCTCCTAAAAATCCTGTAACCAAGCCTAATAGGCAAGAAGCTCATAAACTCGAAGAAGAAGAATTACCTTTAATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAATCGTTTTAAATGTTTAATAATAAAAAAGGTTTGATTGAACAAACCTTTTTTATTATTTTAAATCTTTTAATTGAGGGAATAAAACTACATCTCTAATTGAATCATTTTGAGTTAATAGCATAATTAAACGATCAATTCCTATACCAGAACCGCCTGTTGGAGGCATTCCATATTCCAAAGCATTAACAAAATCTCAATCAATTTCATTTGCTTCTTCATTACCATTTTCTTTTTCCAACAATTGGTTTTCAAAACGTTGCAATTGGTCAATTGGATCATTTAATTCAGTAAACATATTTGCAAATTCTTTTGTTCCAATAAATAGTTCAGCACGTAGTGTAAATCTTTTATCGTCAGGGTCAATTGCTGCAAGAGGAGAAATTTCAATTGGGTGACCATATACAAATGTTGGTTCTATTAATGTTTCTTCAACATATTTTTCAAATAATTCATTGATAATGTGACCATATCTAAAGAATTTTTCAACTTTAATACCATGTTTTTTAGCTAATTCAAACATTTGTTCTTCATTTAATTTTCTAATGTCAACATTAATCTTTTCAGATATAGCATCAATCATATTAACTCTCTTAAATGGAAAATTAAATGTTATATCAAAGCCATTAAATTTAACTTCTTTAATATTGAATTTATTTATTAAGTGTTTGAAAACGCCTTCAGTTCTTTCCATCATTCCTCATAAATCTGAATATGCTTCATAAAATTCAATTGAGGTAAATTCTGGATTATGAGTTGTATCTACGCCTTCATTTCTAAATATTCTGCCGATTTCATAAACTCTATCCATTCCACCAACAATCAATTTTTTTAATGGCAATTCAGTTGCAATTCTTAAATAAAAGTTTCTATTAAGTGCATTATAGAAAGTAATAAAAGGTTTAGCAGCAGCACCACCTAATATTGGATTTAAAATTGGTGTATCAACTTCAATATAGTTCAAACTATCAAAATATTCTCTAATGCCTTTGATAATTTGGGTTCTCATAACAAATGTATTTCTTGTTTTTTCATTCATTATTAAATCTACATAGCGTTGACGACGTCTTTCTTCAGGATCAACTAAACCATGAAATTTATCAGGCAACGGTTTCAATGATTTTGTCAATAGAACTATGTCTTGAGCCTTTATAACTAAAGCATCAGTATTAGTTTTTGACAATGTTCCTCTTACACTTAAAATATCACCAAAATCCATTAAAGAAAAGGTTTTTGCAGAAGACTCATTCAATGTTTTTTTATTTGCATAAATTTGCATTTGTCCGCAATTATCTTGTAAAACAATAAATGGGCCACGAATAGTCATAATTCTTCCATTAAATACAAATTCTAGATTTTGTTTGTCAAGATCTTCACGGCTTAAATTTTTATATTTTTCATTTAATTCTCTTGAATTTGCTGTTGGTTTTATAGTTTTATGAAATGCTTGTAAATTGTTTTTTTCTAATTCAGCAATTTTATTACGTCTAATTTCTTCTTGTTCACTAAGTTTTCTTTCCATAAATATTTATCACTTTCTATTTCTTAAAATATCAAAAAAATTATATATTATATGCTTTATTTTTTTTATAATTATTAATGCATAATTGCGTGGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCATTTTTTTTATACATTTTTAATTAATTAAAAATAGTCCAAAAGGACTATTGGGCATTCTTCATAGAACGCATAACTTGTCTAATTTGTGCTTCCGAAGCTTTTCTTCCCATTTGTTGAAACATTACACGAATCATTTTTTCAGTAACTGGTGGATTTTCTTTTAATTGCTTCTTGAATTTTTTTTGGGTTATATATACCGCAATTATTCCACCGATTATGGCAGTAATTATTGGTAATATAACTAATAACGCTATTCCTCAACTTTGCATATTCTACTTTCCTTTCAATACTGTCTTCTTTAAAATAATATATATTATAAAGGGCAATACAATTAAAACTGCGATACTTATAAAGAAAATTTTGGCATAAAATATGTGAGCTTTTCTTTTACTTTCATCAAGTTCAACAAAATTCATTCCTTGTTCATTGCCAAAGAATGATTGGATGTCCACCATTCTTTTCTTTTTAAAAATTGTACTGAATATAAATAATATTAAAACTATTCCAATTATACCACATAGTATGTAAATTAGTATTTCGGGTTTTGGAAAAGTATTATGAATTCATTTTTGTCAATCCAATAAACCCCATTTTGCATCTTTTAAACCTCAAAAAACGCCTATAAACACACCGATATAAAACACAATAGCAACAACTGCTCATGTTTGTCTTACTTGTCTTATCATTAATTTCTTATAAAGTAAGGCAATAAATGGCGGTGTTGATGTCGCACCATTTTTAATTGTAATCCTATACATATCTATGCTCTTATGTATTCCTGAAAGTTCAATCAATTCAATAGTAAATCTAGTTAATGAAATTACTATAAATATTGCTGGAATTATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTGAATAAATTTTGTCAAAAGTAAAGCAATAATATTGCTATAACGCTCAAAAGAAGGATACCAAAATTTCAAAGTATTGATATTTTTTTAATATTCATTTCTCTTTGAAAAACTGAATAAATTCCATTAGGAATTATTTTCTGAGGATCTTTTATTTTATAACTTAATTGTTGCAAATTTTGAGTATTCATTGAATCTTCTACCATGTGATTTTCAAAGGTACGTGTTGGAATTTCAATGTTTTTACTACTATCGCTATTACTTCCTGTTGTTGTGGGTTTAATATCCATTATTTTCTAATCCTTTCGAGAATCATCTGTTTTTAATATTGCCAAAAATGCTTCTTGTGGGACTTCAACGCTTCCTATTTGTTTCATCCTTTTTTTACCTATTTTTTGTTTTTTTAATAGTTTTTGTCTTCTTGTAACATCACCACCATATAACTTAGCTGTAACATCTTTTCTATATGCCTTAATTGTTTCTCTTGCTATAATTTTAGCACCAATTGCAGCTTGAACGGGAATTTCAAAATTTTGTCTTGGTACTACTTCCTTTAGTTTTTCTGTTAATGCTCTACTTTTATTATATGCTGAATCTCTGTGAACAATCATTGCTAAGGCATCAATTTTCTCACCATTTAATAAAATATCAACCTTTACTAAATCGCTTTCTCTTAAACCAATGTAGTCATAGTCAAATGAAGCATAGCCTTTTGAATAGCTCTTCATTAAATCAAAGAAATCAAATATTATTTCATTTAAAGGCAATTCATAAATTAATAATCTCCGGGTTTCATCTAAATATTCAATATTTACATATTTGCCACGCTTAGATTGGCATAATTCCATAATTGGGCCCAAATATTCTTCGGTTAACATTATATTAGCCTTAATATAAGGTTCCTCTATCATTTTTATATAACTTCTATCTGGAAATAGTGATGGATTTGTAATATATTGAACATCTCCATTAGTTAGTGTTACAACAAACTCAACTGAAGGAGCAGTAGCAATAATATCTAAATTAAATTCTCTTTCGAGTCTTTCTTGAAGAATATCCATATGCAATAAACCTAAAAAACCTATTCTAAATCCAAATCCTAAAGCCTTCGATGTCTCTGACTGTCATACCAATGAAGAATCAGACAAACTAATTTTTTCTAGGGCATCTTTTAAATCATTGTAATCTCTAGTATCAACGGGATAAAATCCAGTATAAACAACTGGTTTTATTTTTTTATATCCTGGCAATGGTTCAGGGCATTGATTTTCAAATAATGTGACAGTATCACCAACTTCAACATCTTTGATATCCCTTATTGATGCTGAAATTCAACCAACTTCGCCAGCTTGCAATTCATTTTTTTGAACTTCATTAGGGTTTTTAACGCCTAATTCAGTTACTAAATAATGCTTTTTTGTTTGCATAAAGTAAATTTCGTCGCCTACTTTTACTTTGCCGTCAAACAATCTCACATAGATAACAACGCCACGATAATTGTCAAAATAGCTATCAAAAATTAAAGCTTGCAATGGTTTATTTATATCTGCTTCTTTAGGAGCAGGAATTTTGTTTATAATTGCATCAATTACTTTATCAATATTTAATCCTGTCTTTGCAGAAATCATAATTGCGTCAGAAGTATCAATTCCAATCATGTCTTGAATCTCTTTTTTTACTCTTTCAGGATCTGCACTAATTAAATCTACCTTATTAATAATAGGAACGATTTCTAAATTATTTTCCATAGCCATATAAACATTAGCCAATGTTTGTGCTTGAATTCCTTGAGTTGCATCAACTAGCAACAATGCTCCTTCACATGCAGCAAGTGATCTACTAACCTCATAAGTAAAATCAACATGACCTGGTGTATCTATCAGGTGAAAAATGTAATCTTTATATTTAATTTGAATAGCATTTAATTTAATAGTAATTCCACGTTCTTGTTCTAGTTCCATTGAATCTAAAATTTGAGGTTTTGCTTCCCTGGCGGAAATTGTATTGGTAAATTCCAAAATACGATCAGCTAAAGTACTTTTACCGTGATCAATGTGTGCTATTATTGCAAAATTTCTTATTTTTTCGTTTGTCATATTATTTTAAATTATATTTAAAAATGTCATACATTTAAAGAAAAATAAAAAATAACCATAAATTAGATACATTTATTAAATATCTCTATGGTTATTTTTTTTAATTATTTTTTGTTTCTTTTACACCAAATATTTGTTCAAATATTTCGTCATAATAATGAACTGGCACAAATTCAACAACATTTTTAACTTCAACAGGAACATCTTTTAGATTCTTATAATTATCATATGGAATAAAAATCTTTTTAATACCAAATTGAGCAGCTGCCAATGATTTTTCTTTTAAACCACCAATTGGTAGAACTTTTCCACGCAATGTAATTTCTCCAGTCATACCGATATTAGCAGGGACTGGTCTTTTAGATAAGGCACTAATTATTGAGGTTGTGAATGTTACTCCGGCACTTGGGCCATCTTTTGGAATAGCGCCTGCTGGAACGTGAATATGAATTGCTGTATTTTCAAAATCAAAGTCAATATTGAATTTGGCAGCATTTGCTCTTACATAAGCTAATGCAATTTGAGCAGATTCACGCATAACATCTTTTAGTTGCCCTGTCATTTTTAAGCCTTCTTTTCCAGGGAATGTTGTAACTTCAATAGCTAATGTTGAACCACCAAAACTTGTATATGCTAACCCATTAACAACACCAATCTGTGCTTTATTTTCGTTTTCATCATCATCGAATTTAATTGGTCCTAAAAATTCAATTACAACTTCTTTTGTAACAACAAAACTATCATTAATTTTTTTGTCAAGAATTTCGACAACAATCTTTCTTGCTATACTATCTAAAATACGTTTTAATTCACGAACTCCACTTTCCCTTGTGTAGTAGCGAACTAAAAATTCTAAAACTTCGTCAGAAATTTGAAATTGTTCAGGATTTAAGGCATTTTGTTCATACACTTTTGGAAGTAAATGTGATTTAGCAATTTCTACTTTTTCTTTAGTAGTATATGTTGAAAGTTCAAGCAATTCAACACGATCAATTAGTGCTGCTGGTATTGAATCATAGTAGTTAGCAGTAGCGATGAATAATACTTTTGATAAGTCGTATTCTAAATCCAAGTAGTGATCTTGAAAATTAACATTTTGTTCAGGGTCTAACACTTCAAGCATTGCGCTTGTTGGATCGCCTCGAAAATCTGATGACATCTTATCTATTTCATCCAACAAAATGACTGGGTTTGAAACTCCGGCTTTTTTAATTGCTGATATTATTTTTCCAGGCATTGCCCCAACATAAGTTCTTCTATGTCCTCTTATTTCGCTTTCATCTTTAACACCGCCCAATGAAACCTTTATAAATCTACGATTTAATGATTCAGCAATTGATTTAGCAAGTGTAGTTTTACCAGTTCCTGGAGGACCAATTAATGTTAAAATAGGAATATTATTTACTGGTTTGTTTGTGGTTTGACCCGTTTTATGAACGAATAATTTAGTATCAATAAATTCTTGATCATTTCCTTTTATTGGTAAATATTCAGTTTTTTCTTCCTTAATTTTAAGATGAGTTAAAACCGAAATAAATTCAAGAATTCTTTCCTTTGGCTTTTCAAGTCCATAGTGATTCTTATTCAATACTTCTCTAACTTTGTTAATATCAATTAATTCTTTACTTACTTTTCTTCAAGGCAATGCCAAGATTAAATCAATATATGTTTTAGATATATTAGCTTCCGGGCTAGATGGCATCATTGAGGCCATTCTTGATTGTTCGGATCTTAATGCTTCTAGAACATATTCAGGATATTGTTCTTTTAAAGTGCTGTCTTTTTCAATGTCGTCAGCAGATTTTCCGCCTTCATCATCCTTCAACATTTTTCTAATTTGACGCATTTTTTCTCTTAGGAAGAAATCTCTTTGTTGGTCTTCCATATCTTCACGTAAATTACTATTAATTTCGTTTTCTATTTTTTCAAATTGAACTTGTTTATTTGCCTTTTTAATTAGTTCATTGTATCTAGCTAAAACGTCATTGCTTTCAATTAAAGAAAGAAGTTCATCACTCAAAAAGTACTTTTTCAAAAACTTTATAAGTTGCCATGATTCAGTTTCATATTCAACTAATGATTGTTTAGGTTTGTTAGTTTCAAATTCTTTTTCTAAATCTCGAAATAAAACTGTTCCTATACCTTCGTTAATAACTTGTTTAGATAGTGAGAAGAATTTTTGTCTTATTTCTTCAATTTTTGGTAAATATGAATCATATGAATTGACTTGAATAGGCGAAATATCAACATAAAAATTATCCTCACTATTTGTTGATTTTTCATCAACTATTGAGATTTTATTAATATTAACCGGTTCTTTTGCTTCTAAATTAACATGATAAATTCCTGATTCTTCACTAATGTTAGTAATATTAACAAGTACGCCTACAGGAGCAATATCATTAATTGTATGTTCCTCATTTGATTGTTTATAATTTGGATTTAAAAATGTAATAACTATATTTTTATTTCCATTAAAAGCCTTTAATAAAGCTGACACTGATTTAGCTTTTCCAACTCTAATTTTGTCAGAAGTATATGGAATAATAATCTGATTTTTTATTGCTATATAAGGTAATTTCATTGTTATCTCCTTAGAAAAATGAATTAATTATTTAATATTTTATATTAAATGTTGTTTTTTATTAGTTTATTTACTAATTATTACTGAGGCGGGTTTTAAAATTCTGTTAAATAATGAATAACCATTGCTCTTTACTTCAAGAATTGTGCCAGAGGGTTCTTTTGATTCGATGCTTTCAGTAATTTGTTGACATTCAGGGTCAAATAAATCTCCTACTTTTGGTTCAATTATTTTTATTCCATTATCTAACATTAAATTTTCAAAATCATTCTTAAGCATATCAAAGCCTTTTGCAAAATTATTAACAGCGATATTATCAGATTTTTTTGCAAAATTTAAAGCACTGTCATATTTTGTATATATCTTAATGAACTCTTCTAAAAAGTCTTGTAGAGCATATTTTTTTAATTCTGTTTTTTGTTGTAATAAATGCTCATCATTTTTTTGACGATGTTCATTTATTTTTGATTGTGCTTTTGCTTCCAATTCAATTACTTGATTTTTAAATAAAATTTCTTGTTGTGTCTTTTCTTGTTTTATTTTTTCATTAAGTTCGCCTAATTCCAAAAAATTTAGTTGCAATTTCTTAATATTTTCATTTAATTCTTGATTTTTAGCAAAAACTTCTAAATACTTAGGACTTGTCTTTTGAAAATCAATAATTATTACTTTAAAAATTTCATTATCAATTTTTAAAACTAACTCACCTTTTTCGTTTTGCTTTCCTTCCAAGATAGTTTTTTCAATATTTTTATCTAAATCAAAGCCAACAAATCCCTTATATTCAATTTTTTCATTTTTGTTAATTTTTTTAATACACTCTATATAATCATATTTTGAAATATTTTGACTCATTTATTCTCCTTTTTCTTCTTCTTTTTTAATAATACCTTCAAATAAATTTATTGCTTCTAGTGATTCTTCAACGTCTAAATTTTTAGGACCTAATATACTTATTTCTTTAATATTTTTATGATTAATAAATTGAATTCTTTTTGAAATAATATTTAAATTAGGATGTGAAAAATCTAGTTTTATATTTTTTTCTTCATCAAGATTGTTTTCGATCGCTTCTCAAACTGAGTGGTTTTCAATTAATTCTAATGTCTTAGCGATCTCTTCTCTGCTTATTTGTTCTGATAATATTATTACATTTTTATTAAACGATTTATTCTCCAATTTTTCTTCAAAACAAAAAATATTTTTGATAAATTTCTGCATTATTAATTCAAAATGTTTAACCTGTGTAGATAATATTGGGGCTAATGATTCAGTTTTTTTAGCAAGATTAATTAATGGAGTATCAATCAATCTTTCTTTAAATAATCTAATTGCAATCCTTAAATCATTTAATTCAATCCCACTTTCAAACTTAAACATTTTGTTTTGAACATTTCCTGAACTTGTAACAATAACAACAACTGCAGATTCTTCATCAAGCGGTGTCAACTGAATACTTTTCAATAATTCAGATTCATTGTTTGAAGTTGCAACTACGGCAAACCCTGCCATTTGGCTAACGACATTTGCTGCTTCTTCTAATGTTGTGTTGATGCTTAACCTACGCTTAGATAAAAGATCTTCAAGTTTTTCATTGAAATATTTTTTAGCATTATAAAGTAAATTTTTTGTATAGTATTCAAGACCTTTTGAAGAAGGAATTCTTCCCCCTGAAACATGTTTTTTTTCTAAAAGACCTAGTTTTTCTAATTCTGCCATTGCGTTTCTTATGGTTGCAGAGCTACAAGATAATTTAAATTTTTCAACTAATCTTTTTGATCCTACTGGTTCGCCGGTTTTAATGTACATTTCAACAATGTATCTAAAATATTTGATTAGTTTCTTATCATAATTATTGCATATAATTAAAGACTTATTTGTGTTTGCATTAGAAGAATTTTGCTCCATGATTGCCTCTTTCAAAAAAATTTTAGCACAATTTAGCACTTTAATGCGTTAATTGCTAATTTTTTAAAGATTAATAGTTTAAAAAAACTAGGAATTAACCTAGTTTTTAATTCAGTTTAACTGATATGGCGCGCCCGGCAGGAGTCGAACCCGCAACCTTTTGGGCCGTAACCAAACACTCTGTCCAATTGAGCTACGGGCGCATTGGAGCCACCAACCGGATTCGAACCGGTAATCAAGGTGTTGCAGACCTATGCCTTGGCCGTTTGGCTATGGTGGCAACACTGGCATGCATGTTTGCATATAGAATTATAATACATTTTATAAATTTTTAAAATAAAATTAGTAAAGAAAAAAGCGCAAGAGGGGACGCTTTAATACTATATATTTTCTTTAAATATGCAATAATTATATTATTTTTTTTAAAAAAATCATTTTTTATTATTTTTAATGCTTTATTTTAAACAAAAATTAACAATTTTAGAACGAAATGAACTTTTTCATCAATCTTAAGACTGTCAATTTAAGCAATGATTCAATCCTAGTTATACCATCATTACCAGGCATTTTATTAAATATTATATGTTGCTTTGAAGAAGTAAAATCAATAGGCGAGCTCATAAAAGTAATTTTATTTTTTTGTTCACGTTCGCTCAATATATTGAATATATTTGTAATATATCATTCAGGTTTTACTCCGATCGCAAAGCGATCTATGAATAAATAATCAACAGAAATCAAATTTTGAACAATTGAATTAATTTCTGATGAGTTTTTTAAATTATTAGACATGACTTCAAACAAATTATTGTTGTCAACAAATGCTACACTTTTGTTTTTCTTAGTGCCAAATAAGAAAGCTAAAAATTTTAAAATATTTCTTGCATTAACGAGATTTTGATCAATGAGCAATAACCCTTGATTTGATTTCATTGAAGTAATATCTTTGATTTCATTTTTTAGTTCTGGTGCAAATTTTTCCAATGATTGGTTTGTATTCAATACTATATATTGAGGTTTCTTTGCTTTTGCGTTATTAAAATAATAAATTCATTCATTATCCATGCTTGTTATATTTGTGAGTCAAAAGTTATTCATTTCTTTTTGTTTTCTAAAAAAACCATCGTTGCTTAAATCAATTTGCAAAATTCCATTATCATCAACAAAGAGGGTCCATGTTGGAAGTTGTTTATGTGCTTCAATATAGCTATAATATTTTTGCAAATAATTCATTCCTTTTTGGATTTGTTTAGAATTTAAATTCAGTTTTTCAATAATTGCTTTAACATTCGGATCGGCTAAAATTTTTGCTTTTTCAGCCTCTAAATCAATATTTTTCAAAAGAGAATTTTTAAACAATTCATCAAATATTTTGTTTTCCATTTTTTTGCTTTCTTATTTTTTAGTTAGTTCAATCATGTTCATTAGCGAGATCTAAAAAACAATTTAAATCTTCTCTAGTAACTGATTCATCTTCATTTTTTTTGATATTTTTTAACTCTTTATTTGCATTTTGTTTTATTAGATAAACATTTTCTAATTCTTGATCAATTCTTTCAGCATCATATAAATTTCTATGACTAAAATCTTCGGCTATTTTTTCAATATATTTGCATACAACTGAATGGTTAATTTTAATTGAATAATAAATGAAGGCATTTATTGAAAAATTATTGAATTTCATTCTTTTCAATTTTCTAATCATTTCATTTTGGCTAAAACTTAAATTTGTTTTTGTATATTGACTAATAAATTGTTCGGGTTCTATCTTGTCCTTTAATTCATCTAGAGTAATTGAATTAAAATTCAATTCTTCATTTTTTGCATTTAAATCAATTATATTTTCTAGATTAAATACTTCATAAAATTTTTTAGAAACATCATTAATTGTTGTTTTATCAAATTGATAATTTGCATTTAATTTTAATAATTCTTCATATTTTGATTTAGTGATTAATTTAATTAACATATTAGAAATAATTTTATTATTGCTTATTTCATTTGCATTCAAAGGAGCATTTAAACTAAAAATATAAACACCATCCTTGTTTAAATAACTTTTTAGTAAGCTTAGCGCTTCTAGTTTACTACGACATTCCTCAAATTCTTGTTCATTTAATAATAAAATATCTTTAATTTCAGAAAAATTAAAAAGTTTATTTTTTGTATAAATATTTTTTAAATTATATAAAAATTGATATAGAAATATGCTACTATGGCCTAAAATCGGAGCATAAAAAATTACTAGATTTTTAAAATCGTTTTGTGTAATTTCCCTCTCGCTTTCAATAAAAAAATTTTCAACCATATTTAGCCCCTTTCTAATTAGTCGTGTACAAATATGATAGATTGCTTAGGAAATAAAAATTAATTTTTCTTAGGTTTTTTAACAATTTAACTACTTATTAAAAAGTTATTAACAATGCACCTTATGCCTTTTAGCCTTAAAACTAGATACTTTTTAAACTATTTTTATTTTCTAACAGTAAGAAGGAATTTTTTAATGATTTTTTGGTTAAAAAATGTAATAAAAAATAGAAAAATTTTTTCTTGCAAAATAACTTTCACTATGTTTTCTTATAGTTTCGAAAAATGTTTTATTTAATAATATAATTGAAATATGAAATTAATTAACCTTCACTTAAATACTATATTTTCTTTTTTAGAAAGTACAATAACCATTAATGATTTTATTCAAGAATTACTAAATAATAATATAGAGTATTTTGGTATTACAGAACATTGCAATTTTTATAGCATGCCACATTTTTTAAATGAAGCTAGAAAACATGATTTAAAGCCTATTTTTGGTCTTGACGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTATGGAAAAAAAGGCTATATAGATTTAGCTGAAATTAGAGATTATTCTAATTTAATATGAATAGAACATCCAATTTTTGGCTATTATAAAAAAACTAATAATATTTTAGAAATTGAAAACCATTATTTTGCAATAAGCCAATATGATATAGACAATAGTGAAATACAAAATATTTTTGATAAGTGTTTATTAATTAATCACAATTCAATATTAAAATATGATGATAATTCAATAATTAAAACAATGTCAAATATTTCAAATAATAAAATATTTGAAAGCATTTACGAAGAATTTAAAGTTGATATTGAAACAAAAAATAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAAAGGTTTCAAAATGCCCAAATTCAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTTGATAAATATTCATGAACAGATGAATACCAACAAAGGTTTTTAAAAGAACTAAAAGTTATTGAAGATCTTAATTTTGCTGATTACTTTTTAGTTGTTCAAGATTGAGTTAATTGAGCAAAAAATAACAATATTAAAATAGGACCAGGAAGAGGTTCAGCAGCCGGTTCATTAATAGGCTATCTATTAAATATAACCGAAGTAGACCCTATTAAATATGATTTGATATTTGAAAGGTTCCTAAATTCTAATAGAGTTACTATGCCTGATATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTCGTGGCAAATATTGTTACTTTTTCTTCACTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTATTAGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCATTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACACATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCAATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGCATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAGACATTGACAACTATTTCAAATATAGTTGATCTAATTTATCAAAATCAAAATCTTAAAATTGACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAATTATTGAGCTCTGGCCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGACAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCAGGTCCAATGAAAAACGTTTCTGTTTATGCAAAAAGAAAATTTGGACTAGAGTCGATACCAAAAATAAATAATGAATATGATAAAATCCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATTATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCTGACAATCTAAGAAGAATTATCTCTAAGAAAAAAATAAATGAAATGGAAGATATTAAATCTATTTTTATTGAAAGAGCAATGAAGAATGGCTATGAAAAGTCTTTGGCCACGAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTCAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCATTACCCCTTAGAATTTTATACGTCATGTATAAGCTCGGCACACGGCTCTCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGTGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAAGCTATAATTAAAAATAGAAAAATTATATTGCCACTTACACTAATTAAGGGTCTTGGTCCCGAAATTGCTAATTCAATTGTTGAAAATAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTACTAATAAAAGCAAACACTCTTAGATCATTTAATTATAATCAAAACACCTTGTTGAATGAGATTCAAAAAAACAATACTGATACAATGTTATTTATAAAAATGTTTAAGAATAACATTGAAGGTGTTAGCTCTAATATAATAAACTCTTATAAACCAAGTGAAATCCTTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTGTTAGGTCAAGTTTATAATTTTAATTCAAGCGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATTGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATGTTAAGGGAAAAACTATCAAAGTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCCTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCAGAAGGCTTTGAAACCAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATTTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCGCCAGAAAGTTTTTATCTACAGCTTGATTTAATTCAAGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAAGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAATTAATAGATGAACAAACAGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTACCAAATCAAGTTATTGATTATAAAGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGTCCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACATTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAGAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTTTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAACAAAAGTAGGGTTTTTCCTACTTTTGTTATTTTTGAAGTTCTTGCAATGTTTTTGCTACTCCAGATTTTTTATATGAATATCCTACATAATCTGCTTTTTGTTTTATGTTCTTTGTAGAATTTTTCATCGCTACAAAGCCACCTAAATATTTTTGAGTAACAACATCATTGCCCGAATCGCCTAAGTGAACTGCTTCTTTATAATCAATATTTAACAAATCACATACAAATCGATCGCCAATTCCTTTATTAGCATTAATATCGCTAATTTCAATTGAATATCCTTTTGATACAATATGTGTTGAAATATCATGAAAAGCATTTAATAATTCATCTCTAAGTTTTATTATTCCTTTTTTTGACTTCCCAAAAGTTAAAATTTTTGTTGTTGCTGGCAATGATTGAATTTCATCATATGTAACTTTGTTTAGCTTCTTTAATCAAGCTCTAGCGATTCTAAATTTTGTTTTTGTTCCATATATTGTATCGCCACTATTAAAAATAAAAAACATTTTTTGTGCAATTAAAATTTCAATTATTGATTTCAATGTATCAGGGTTAATTTCATGGCGTTTCAAAACATTATTATTTTTATCTACAATTACTCCACCATTTTGGCAAACAGCAAACGGTGCATTTATTTTATTAGTTAAATCCAAAACAAACTTTGAATTTCCTCTACCTGTTGACAAAATAACAGGGATTCCTCTATTATTGAAGTCCTTAGCAACCTCAACATTTTTTTCGCTTATCATAATTTGTTTTTTGCGAGGCAAATCTAGATATGTGCCATCCATATCCAAAAAATATGCCTTAGGTTTAAAATTAATCATAATTTTCCTACTTATTTAAATTGTTTAAAATTTCTTCAACTGGCAAGTATCCTACACTTTGAGAAACTAACTTACCGTCTCTATAATAAAATAGCGATGGAATATGAGATACATTCATTTCTCTTGCTAAATCAATGAATTTATCTACGTCCAAATCAAAAACATTAACATCTGAATTTTCTTCTGCTAATTTTTCTAATTCTGGGCCAATCATTTGGCATGGACCACATCAAACTGCTCTAAATGCAACAATTGATTTTCCTTCTAAATATTCATTCTTTTTGAATTCTTCAACTGATTCAATAGTTTTATACATAATATTTTCTCCTTTAATATGCTCTTGCAAATAATACAAATCTTTTTGTTTTTCTATTAGTTCAAAAACAAGTATCATTTGAAACTTTTATTGGTTCTTTGAATGGAATACACCTTGTTGATGCCCCTGTTTCTTCTTTAATTTTTACTTCATCATCACCGTTACCATCAAATGCAGCATACACTCAATGACCCGCAGCAATTGCTTCCTTAAATTCTTGATAATTATTAACAGTTACAATATTATTTTGTAAGCGTTGTTTAGCACTTTCAAATAAATTGTCTTGTATTTTTTGTAATAAGAAGCTGGCAATATCTGCTGCTTCAGCAATTGTTGAAGTAATTTTTTCTAGGTTATCACGTCTAACGAATAATACACTATTATTTTCAATATCCTTTGGACCAATTTCTATTCTCAATGGTGTACCATGAATTTCTGAATTTGCTGCTTTAAAACCTGGACCTTTGTTTGAAGAATCTATGTTTGCAACAATATTTTTTGCTAATAATGCATCTTTTAATTCAAGGGCAAGCTTGTGAACTCGTTCATCCTTTGAATTAAAAATTTCTAATATATCAACTTGAGTCCTTGCTATTTTTGGTGGAATGATTATTCCTCGATCATCACCATGAGTCATAATTAGCGCACCAATTAATCTTGTTGTTGTCCCTCAACTGGTACCAAAAGGATTAATTAATTTATTTTCATTATTTTTGAATGTTATATCGAATGCCTTAGTAAAATTTTGTCCCAAATAGTGGCTAGTACCCGATTGAAGTGCCTTTCCGTCCTTCATCATTGATTCTAAGGTGTATGTTATATCAGCGCCAGCAAATTTTTCATGATCCGTTTTGCAGCCTTCTACTACTGGAATTGCTAAATTTTCTTCAATAACATCTTTATAAACCTTGTGCATAGTCTTGGCAAATTCAATGGCCTCTTCACTACTTGAATGGATAGTATGACCTTCTTGTCACAAAAATTCCCTTGTTCTTAAAAATGGATTTGTTGTTTTTTCTCATCTTAAAACATTAACTCATTGATTATAAATTATTGGCAAATCATTGTAAGATGAAACTTCATTTTTGAATAAATCAGCAAACAATGTTTCTGAAGTTGGCCTTATAAAAAACTTTTCTGCCAATTTTTTGCCGCCTACTTCAGTAACAGTTGCAAGTTCAGGATTAAATCCTTCAACATGATTTTTTTCTTTTTGTAATAAGCTTTCTGGTATTAAAAGAGGTAAATAAACGTTTTGAACGCCTCTCTTTTTAAATTCAATATCCATTTTTGCTTGAATATTTTCTCAAATAGAATATGCCAATGGTTTAAAAATAATGCATCCTTTTGTTGCACCATATGCCATTAAATCCCCATTTTGGATAACGTCAGTATATCAACGCGCAAAATCTTCATTTTGTGGTGTTATTTTATCTAATTTTTTCATGTAATTAATTATAAATAAAAAAGATAAAATTAAAAGCACAATGTGCTTTAATTTATTTTTTAGATTAATTTATCAAAAATATCTTCACCTGTTTGAACGGTTGGAACATTGAAATTTCTTGCAACAATATTTCCTGCAGTTCCCAAGCCTTTCAAAGGTTCTTTAAATACACATGGTGATTTGAATTTCTTTGAAAATATTGTCAATGGCAATGCTTCTCTTGTATGATCAGGGCCTTTTGTTGGGTCATTTCCATGGTCAGAAGTTATAATTAACAAGTCATCTGATTTCATTGCGTTTATCAATTTTGTTAATTTAATATCTAGCCTTGAAATATTTTGTGAATATCCTATTGGGTCTTCTCTATGTCCAAAATGGCTGTCAAATTGAACAAGGTTTACAAATACAAATTGGTTTTTGGTATTTGAAGAAGCAATGTCAATTGCAACGTCCATATTTTCATCATCAGAAGCTGGTCCATAAACTTTGTCAATACCAACATGACTGAAAATGTCGTTAATTTTACCAACAGCAACTACTTCAACACCTGCTTTTTGTAAATCTTCTAGAATAATATGTCCTGTTGGTTTATTAGCAAAATCATGACGATTGAATGTACGTGTGAACTTTCCATTTTCATAAACAAATGGACGTGAGATAACTCTTGCTACATTTCATTCAGGTCTTGATGAACAAATTGCTCTTGCTCTCTTTGCATATTCAGTTAGTTTTTCAACACCCAATGTTTTTTCATCCCCAATAATTTGCAATGTCGAATCAGGAGAAGTGTAAATTATCATATCTCCTTTTTCAATGTGTTGCAAACCTAATTCGGCCAATATAACTGTGCCTGATGCATTTCTATTTCCAATAATTTTTCTATTATCAAACGCTTTTGATAATTCTGCGACTAAATCAGCAGGGAAACCTTTTTCAATGAATTGTGGGTTTGGAACTTTTGTATAAATTCCCATCATTTCTCAATGTCCTGTCAATGTATCTTTTCCATTTGACATTTCATAAATTTTTCCCATATATGCCAATGGTTTTTTATTTCTTGGTACATGTTTTGAAGTTTCTGCTATTTCAGTAATTCCTAATTTTGTTCATGTAGGAATTTCTAGTGGCAATGCTTCTGATGCATGCAACATGCTATTTGCACCTTCATCATGAAATTCAGCTTGGCGTGGTTCATATCCAATGCCAAGACCATCTGTAACTATAAAAAATATTCTATTAAATTTTTGTTCTTGCATAATTAGTACTCCTTTTTAGCAATCACAATAATAATTGGAATCTCATACTTATTTTTTAAATTGATTTTAATTTTAAATAAAAATAATTAATGTTTTAATAAGTTAGATAAGTAATTTTACTATTAATTTACTCACTTATTATTTTATTACAATAATGAATTTAAATTTATTAACTTTCAATGCATTAATAGACTTTATATTGTCCGGCGGGCATTCTATTGATTTTTTTAGCCTCCTGTGTGATAAAATTAATATGTTAGATTTATATATTTATTTAACAAAAGAGGTAATATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGCGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACATTCCAATCTCATTAATGCTTGATACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAAAACGGATGAAAATTCATACAAGAATTTTTTGGGTACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAAAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAGGATGTTAAAAAATTCGAAATTATTGTTGAAACAGTTAATGAACATTTCTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAGGATATTGACGATATTATATGGGGTGCAAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAATGCAAATGGCGGAGAAAACATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATTAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTAGCAAGAGGGGACCTTGGACTTGAAATTCCTTACTATGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTCGGCAAGGAAGTTATAGTTGCAACTCAAATGTTAGATTCAATGGAAAACAACCCTATTCCAACAAGAGCGGAAGTTACTGACGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCTGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTGGCAAATCATCCTACTTCAAGAATGTTAATTGCTTATGAAGTTGCAAAAATCCTTCAAAGCGGTGAATACAAATTTGCTATTATATTAAGTCACACCGGAAAATTATTAAAAGAAGTTGCTAAATACAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTGGTTTGAAGTTTTGGAATAACTGCTGGTGTATTCTGTGAAAAACATTCAGAAGCAGTAAGAGCATTAATCAAAAACAATCACAATGCTGCTTATAAAATTTTAGGCGAATATGGTGCTAAAAAAGGTGACAAATATATTGTAGTGGATAGTTTAAATATCACAACCCATGAATACTAATAAAAATTATTAATTTCAAAACGAAAAATTAGGCTTTAACTAGGCCTAATTTTTTTAGTTATATTACTTTGAAGATTTTAAAAAGATCCTTGATGTTATTTGATATAGAAAGAAATAAAAAATGTTGTCTATAAAAACAACATTTTTAAAATATTATTTAATAACTTCTTTACCCATGTATGGAACTAAAACTTTTGGAATTGTGATTGAACCATCTGGGTTTTGATAATTTTCTAAAATAGCAGCAACAACTCTATCAATAGCTAAGCCTGAACCATTCATTGTGTATGCATATTTTGTGTGACCATTTTCATCACGATATCTAATTTTTGCCCTACGACCTTGGAAATCTCTACATATAGAAATTGAGCTTGTTTCTCTATATCTTTGTTCTGATGGAATTCATAGTTCTAGGTCAATAGTTTTTGCTGAGCTAAATCCAATATCGCCAGTTGAAAGCATCATTTCACGGTATGGAATTTCAAGTAATTCAAGAATGCTTTTGGCATCATTTACCATTGATTCTCATTCTTTCATTGCATCTTTTTCACTGGTTATTTTTACAAGTTCGACTTTATGGAATTCGTGTTGTCTAATCAAACCTTTTGTATCTTTTCCACTGCTTCCTGCTTCTGAACGGAAACATTTTGTATATGCAACAAATTTCTTTGGTTTAGACAAATCTAAAATTTCGTCATAATAATAATTTGTAACCGGAACTTCTGCAGTTGGTATTAATCAAAGGTCATTGCCTTGCATTTTAAATAAATCTTCTGCAAATTTAGGCAATTGTCCCGTTCCATAAAGCATATTTGAATTAACTAAATGGGCAGGCATTAATTCTTTATATCCATTTGCAATGTGCGTATCAAGCATAAAAGAGGCTAAGGATCTTACTAATTTAGCACCATCATTTTTGTATAAAACAAATCTTGTGCTTGCTAACTTCACTGATCTAACAAAATCAACAATATCTAATTTTGTTGCTATTTCATAATGTGGCAATACGCCTTTTACTAAGCCTCTTCCTAAATTTGGATGTTCTTTTAAAACTACGTTGTCATCTTCGTCTCTTCCTACAGGAACTTCATCATATGGCAAGTTAGGAATTTGGTATAGCAATGTGTTGACTTTCTTTACCAATGTGTCAGCTTCTTCATCAATTTTTTCAATTTGTTCTTTAACTTCAGCAACTTGTTGTAATAATTCTGAGCAATCTTGCTTGTTTCTTTTATTAATTCCAATTTGTTCACTTAATTTATTTCTTTTGGCTTGCAAAGTTTCTAAATTATGAATTAATTCGTTTCTTTTTTGAGCGTCTTCAAAAATTTCTTCGATAATAGCAATATTGAAATTACGTGTTGATAGTTTTTTAATAACTTCATTTTTGTTACTAATAATATATTTTAAATCTAACATTTTTAATCCTCTTTTATTTTTTTAAATGCTATTATATTTTACAATATTATTAAATAAAAGAATTACTTAATTTTTGTTAAATGTTATTTGACTTTTGCTTATTTTATATATAAAACAATAATTGATTATTATTGATAACCAAATTCTTATATTTGTTAAAATTTTAAATATGTTACAAAATAGCAATATTCAAGGTAAATTTAATAAAATAATGTGAACGTCTACTGATGGCCGAGTTGTTATAGTTTCATTTTTAGTATCTTCTAATGATGGTTTGAATCCTGTTAAAGTTAATAAATACAATAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACCTTAAACAGCCACATTTATGAAATATCAGTTTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAACTATGTTATTAAATTTTTAAAAAGTAGTCATTTTCCTGGTATAGGCGAAGAAAAAGCAAAATTAATTGTAAAAGATTTAGGGATCGATGCTTTAAGAAAAATGGTTGAGGATAATTCAATTGATTATTCAAAATATTCAATATCAGAATTGGCATGAAACTATGCTGTTGAATTTATTAAAAATAATCCAACATTAATTGAGGATCAAATGTTCTTTTTAACAAATAATTTAACGCCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTATAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGTATCTCACTTGAAGATATGGACAAATTACATAATATCTTTAACAAATTTGAACACCCCTTTAAACCTGCAACCCACTTGTACAAATCATTTTTAAAATATCTATATAATTCTGGAAACACTAGAATTTTAAAAGATTATTTATACGACCAGCTAATAAACTTTGCAAGCCAAGACACTGAATGACCTAGGGATGTTGAAACATTCAATAATAGCGTTGAATTATTGGTTCAAAATAAATATTTAATCGAAATTGAATACGAAGATGGTACATATCTTAGTGCCAAAGACGTATTTGATATGGAGAAATATATTGTCAAAAGATTGAAACATATTGAATCTGCTTCAAACCCAAAATTCATTAATTTCAAGCCAAGTGAAATGTTCCACCCTTTGCAACTTCAAGCAATTGAAACAGCATTAACTAGCAAATTGACATTGATTACAGGAAACCCCGGCACTGGAAAAACATTAATAACTAATGAAATAATTAAACAACTGCTTTCGGTCTATGATGAAGATGATTTGGCAATAGTTACACCAACAGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAAACAAATGCAGTTACTATACACAGTTTTTTAGAATGGGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTGGATTTATTTTATTCACTACTTAAAGGAATAAAAAAGAATTCATTAAAACGAATCATATTAGTTGGCGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGATGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCTCAGTTTATCGAAACAAAACAGAGTGATCTAAAGTCAATGTTACTCTTTAAATTAAATGACTTATTGCAACAAGGCTATAGCAAACGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGATCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAATATAGAGACCATATTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCTTTTGAAAAAATTTTGGCAAGTATCTCTGTAGATTTTGAAAACGATTCAAAAACTGTTTCTTATTCTAGAAAAGATTTTTTTGCTAATACATATCCTGCATATTGCACAAGCGTTCATAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCAAAAAAACTTATTTATACCGCTATTACTAGAGCAAAAAAATATTGCGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAATGAATAATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCACAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATTGACAAAGAAGTTATTTTAGCCAAACCGTTAACATTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTGCCAGTTGGAAAAGCTGTTATTAGACAAAAAGGTTCGTCTGGTGGACATAATGGAATGAAAGATATAATTGCTAAATTAAAAACTGATGAAATAAAAAGATTAAAAATAGGAATTGGACGTGGTCAGAATGTGATTAATTATGTTCTAGGTAAATTTAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGATATTGCTTCTGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAATTTAGTTAAATTACAACGTGACTTATTAAATGAATTTAATAAATGCAATATTGACGTCAATAAAAAATTTCTAATTGGGGTAAGTGGTGGCCCTGATTCAATGTGGCTACTTAATTTACTTAAATCATTGAATATTGTTGCTGCGCATGTAAACTACAACAAAAGATATGATAGCAATTATGATGAATCATTGGTTTCACGATTTTGTGCTAATAACAATATCCCTTTAGAAATACTTTCATTACATAACAAATATTCTAGTGGTAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAATTTCTAAAAAATATCAATGCGACTATATCCTCCTTGCTCACCAAAAAGATGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCCTTATTTTGAGGTATTAAGCAACTTAACATTCTAAATAATAATATTATTTTTAGACCAATGTTAAATTTATGAACTAAACAAGAAATCATAGACTTTTGCAATGAATATTCAATATTATTTGCAACTGATTATACCAATGCTGAAACTCATTACACTAGAAATAAAATTAGATCGAACTTAAAGAAATTAGATCCAGAGCAAAAACAAAATAAATATCAATTTTTTGTCAATAAAAATATTGAAAATGAACATATTGAAAAACAAGTTTTAGAAAGTTTTGAAGATTGAAAAAATAATCAATTCAATGTTGATAAATTAGATAAGAATTTTGTTTATATTGACCAGTTATTGTTTTATACATTAAATCATTTTTATAACAATTTAAAACTTTCATTAAACAAGATACACGGAATTAAAGATTTTTTATTTTCACAAAATAGAACAAAAAATTTTAAATTAAACGATGAACAATATATTCATAAAATTAAAAATTTACTAATATTTAAGTAATTTTATTATAATTTACATATTAAATTATAAACAAATAGAGAGGACAAATGAACAATCCAAAAGAAAAAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTTATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAATGTTATTAGAGGAACTGCTAACAAAAATGATGGAAACTATATTTCTTCATGAGTTGAAGACCCTTATAACGATACAATAAATATCACTATTAGAGGCACAAATAGTTTTTTAAGATCCTTAATTGGGAATAATTATTTTATTGGCGATTCTTATAGTTTTATAGTAAAAAATGGAGGCTATATAGGTCGTTCAATATTTCAAGAATTGCCTGTATGAGCAGGTTTAAGTGGAAAGTCATATATAGAAGCATTGAATTCATGTGCAACATTTAATGGTCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAGCCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTTGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGTAAGGGAAGAGGAATGCTAACTGGCGGTATTGATACAAATAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCTGGCGCTAGAATTCCAAAAGGAATATTACTAGGAGGCCCTCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAACGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTGGGTGCAAAAAGAGTTCGTGACATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGATGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGATGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAGATGGATGGTATCAAAGAAAATAGCGGTATTTTAATAATGGCTGCTACAAATAGAACCGATGTTCTAGACCCTGCTTTATTAAGACCAGGACGTTTTGATAGAACCATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATCTTAAAATTACATGCTAATGGAAAAAGAATTGCAAACAATATTAATTTTGCTATGGTGGCAAGAAGAACTCCTGGTTTCTCTGGAGCACAACTTGAAAACGTAATTAACGAAGCCTCTTTATTAAGTGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACCATTTCTGAAAAAGAAAACATTGCAGTTGCCTACCATGAAGCGGGACACGCAGTTGTTGGAATAAAAATTAAAGGTGGAAATAAAGTTCAAAAAATAACCATCATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCTGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCGTTTATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGACGATATTGCTAAGGCAACAAATATTGCTAGAAAAATGGTTACTGAATGAGGTTTATCAGACCTAGGACCAATTAAATATGAAGAAGATTCTTCAAATCCTTTCTTAGGAAGAGACTACATGAAAAATTCTTCATTTTCAAGTAAAGTAGGTCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACTGCTGAAAAGAAGGCTCATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTTGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTACCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAATGATTCAAGCAACCATTCAGAAGATTCTAATAATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAAAATAAAAAAGTGGAATTCCACTTTTTTATATATCTCCGCTTTCTCGCAACATCTTTTCAAGGCCGTTAACAAGTTTTTTAGAAAAAACTCCTGTTCCTTTTTGCTTTTGTTCATATAAAAAATCTAATTCTTCATACAATTTATAAATTAATTTCAATGAATAATTGTATTGACTAATTGAATTTTTAAAATCTTGCCAATCTATAACGCTAAAATCTTTATCATCATAACTTTTAATATCGATATCAAAATCAATATATTTAATTACTTCATTTTCAATATAAAAAGGGCTTGCTAAATTAATATAAATATAGTTTTCTTTAGATTTATTTAATGTAATAGAAGCATTATAAAATTTGTTTCTAGCAAAGAAAAACAAAATAGGTTCAGAAACAACTCAATTGATAGATTTTTCAGAAACTTTTGTTTTCATTAACAAAACAACTACAAAGTCAGTTAAATTTGCAACTATTTTTGCCCCTTCATATTGTCTGTATAAAGTTCCATCATATTTAAAAACTTGAATGGTTGTTAGTTGTCCCATCTTAAAAAACTTTTTATGTTTTTTTATATTTTTTGAGTTTGGTGTATCAATTGTTTTTAATAAATCATCAAAACTCTTTATACTTTCTTCTTCCATAATACTATTTAAATGGTATCAAATAAAAAACAAAAAGATTAATTAATTAAATTTTATTTTTAATATATTACAAATTTAACAACTTGCGATTTGCTGTATATGTTATAATAATTACGTGATTTAAACAAATTAAGCATTTTAGAATAAATCTATGTATGCTTTGAAAAGTAACCTTTGGTTGCTTTTTTTGTTTATTTAGTGTATTATAATTAATATGCGACTAAGATTTAATAAAAATGCAGAAACATCCTTAATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAAAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAATGCACCCTGACATAGAATATATCGGACTTGAAAAATACAGCACGCCCGCCTATTCTGCTTTAAAAAAAGCAATTGACCTCAACCTTGAAAATTTTCACATAATTATAGGAGATGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGATTAACATTTTCAGATCCTTGACCTAAAAAAAGGCATTATAAAAGAAGGTTAGTTTATAGAGATTTTTTAAAAATTTATCAAAACATTTTGGATAAAGATGGAGTGGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATCAATGCAAAAATCATTTATCAAACTTCTGATTTACACCATTGCAATTTTGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCGTTTACTTTTAATTAAAAAGGCCTGTAATAGAGGCCTTTTTTGTTATAATTATATAATAATATAATTATATTGTGTAAATGGAGTAAATTATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAATAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGACGAAGAGGACGAAGAAGAAATCCCTCAAGACAAAGAAGGCTATAAGGTAGAACCCCAACTTTTAGATAAAGAAATTAATGGTTTAAGACCCGCTAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCACGTGCTCTACCTGATGCTCGTGATGGTCTAAAGCCAGTACACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCAGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCTTTAATTGATGGACACGGTAACTTTGGATCTGTTGACGGAGACGAAGCTGCTGCAATGCGTTATACAGAAGCTAGAATGAGCAAGATTGCTGGCGCAATGGTTGATGGTATTAAGAAAAATACTGTTGACTTTATGGACAACTATGACGCAACTGAAAAAGAACCAGTTGTTTTACCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTAGGTGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTAATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATTATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCAAACAGAGATGGCATTAGAATTGTGATTGATATCAAAAAATCTTTTGTTCCCGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGCTGGTAAATAATGAACCAAAATTGTTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGATTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTCTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCAAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTAAGTGAAATACAAACAAAAGCAATTGTTGATATGAAGCTAGGAAAATTAACTGGATTAGCAATTGAAAAAATGAACGAAGAACTAAATGAAGTACATGAAAGAATTGCTAACTACATTAAGATATTAGGCGACCATAACTTATTAATCAATTTGATAATTGAAGAATTACAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGAGATGAATTGAGCAGCATTGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACATTAACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTTGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTAATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGCCACGAAGTTCCCGTTGGCACCAAACAATCAAAGGGTATTCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAAATTTTGCCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACAATTTTATTTGAAAGTATTAACAGAAATGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTTAAAGACGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGGTCAATGGGAAGAATGGCCTATGGTGTTAAGGGAATTAAAATTGACGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTTGGAAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACTAGACGTGGAGCAAGTGGAGTTTATTCATTAAATGAAGAAAAATCTGGTAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTAATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAACATTGTTGCAGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCCAAAAATAACCTTAATCAAAAAAATGAATAATTATTAATAACAAAATAAACTATAATTATTATGTATGCGTTAAAATGCATACAGATTGTAGTTGCACAGAGAGGTTTTGAAAAATAATCTCTCTTTTTTTATATTTTAAAAACCACCTTATAAAGGCGGTTTTTAAAATATAGATATATGTTTAATTTTCTGGGTTTTGTTTTAATTCTGTAAGAAATTTCTCTAATTTTTTATTTAAATCAATCGATTTCAATAGTCAAGGAGTTTTATCAACTCCCTTAAAATATTCAAACATTCCTTCAGGTGCATCTTTAAGTCCAAATCATTCAACTCATTTATTGATAGATTCTAATTGATTTGAATTGTTTGGTAATTTTAACGTTAATTCGTCTAATTTAGTTTTTCATTCAGAAATTTTTGAGGCATATTTTCCACTTTGTTCTAAGCGTTTTATAGTATCATTAACTTCTTTGTATATTGATTTATTTGGATCAATTCTGGTCATTGTCGTAGAATAAGGTCCGATCTTGCGAACTGTTGTTGTGGCTTTGTGTTCTTTAAAGTTTGATAAAATCTTTTTTCAAACAACATCGCAATAAAATCATAATCTTTTTTCAACCTCTGACATTGTTTTATTAAAGTTAGATACATATTCATCAAAGGTCATATTCTTAAATAATATTTTCTTTTCTTTTGTTTCGACTACAGTGTTTTTAGATTTGTCAATTTCGTATTGGGATTGTTCAAATTTAACAGCATTTTTAGCAAATTTATTATTTCATTCGGATGAGATACCTTCAAAATAAAGTCCATCGATTTCTTTTAAAACCTCACCACGAATATATGGAGCAGTATTTCAATCGTGAAATAGTCAATTAATCCTATCTGTGTTTATATTTCCGTCAAATATTTTTATTAAATCTTTAACAAATTTTTCTTGTACTTCTTGTATATCATTTTCATATTTTGACGCTTCTGCAATAGTTGCCAATTGACTAGAATTATTTTTAATATTTGTTAATTCTTGCAATGCCTTTTGTTTTATTTCATTTAGTTGTTGTGTTACACCTAAAAAATCTGGATCTTTTACACTACTATCTAGCTTATTAAATTTTTGCAAACTATTATTTATATCATCTAGACTATTATTTATTAATAATTTTTTAGCATTAAGATATTCAACTGAAATTTCATTAATTACTTGTATTATTTCTTCTCTTGTATGTAGAGTACTAATATTATGTTTTCTAATAGATCATTCTATTCTTTCAATGTATGCGTTATATTTATCTCTAGGGCGCATTTTATTTTCAATATATTCTTTTGCTTCTATAATTAAAGAAATTAATCTGTCCCTTAATTTTTGCATATCATTTTCAACGAATTCTTTACTTTGCCTTAAAATTTCGTTCAAATGAATATTTGTAGCTACTATTTCTGATTTATTAGAACTAATTGAAACGTTTCCTCGTCTTGAAACTTCTCTATTAAGCTTTCTAACTATATTTGCATAATAATCAGATTTAACAACATCTTTGGCCAAAAATTCTTCAACTAATTTTCTAGTTTTTTGTAATTTTTCAAATTCTTGTTCTTTTGATTTTTTAAATTTGATCAATATATCTTCAACTTCAGAAATTTTAAAAGTTAATGATTTATTTAAATTATTTATTTCGTCTATATTATTTTTGTTATAAACCTCTAATGCATTAATAATTTGTGAATTTAAATCTTCCTTAGTTTTTTGAATATCATCATCATTATCAATTAATTTATTTTCCAATATCCTCATAGCATGAATAATATTTTCCAACTGTTTCTTTGCTGCAAAAAGTAGTTTTTGCATGCTTTGCTTTTGAATTTTTGCATTTAATGAATCAATCGAACTTTTAATCTTAAAAATTTCTTGATTAATATTATCAATTGGCGATTCAATATTGATCATTTTGGAATTATCAAAGACATTTTGTTCTTCAATTGAATAAATTCCTTGAATGTTTTTATTTTCTAGCAATAATTGCAATTCTTCTTGAAAAATTTTAAATTTTTTAAATAGTGATTCTTTTTGTGTCTTTTTATCCACTTTATTTTGACACGATCTTATTGCTACGGTAGCGATGGCAGCTATTGATAATCCGCCACAAAAAATTGCTAATGGAAGAACGATCTTTTTGCTTTTATTGTTCATTTTTTTTCTCCATTTTTTTATTATTTTTAATATTTGTAAAATTATTTTATGTTATTTAGTGCATAAGAATATTTTTATATTAAAATAGCAGGAATGAGATCATATAAATGGAAGAAAAGGTTTATTTTTTAAAAAAATACATATAGATTAAGCAAATAATTTATTATCGATTAATATGAATATATTTTACTTGTATAAAATAATATTCTTTGCCTAATCATAATTATTATTGGATAGAGTACGAAAATAACTGCGGTATATATTGGTATTTCAATAAGAGTTTTTGCTATTATTGGAATCATAAATATTGGATAATAAGTTTTGTAATCTCAATTGCCTGATCTAAATCTATTATGATAATTTATATATGCAAATGGTCCTCAAAATCATCTACACAACATTAAAGTGAAAAACACAACCAATAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAGGTTATAGAAGATATTCAAATAAATGCCAATCCTACAATAGCAATAGATAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGAATTGCTAAAACTATTACAAAAATTGCTGTTATTATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAGCCACTTATTAAAGCAATAAAAACAGGCACTAACGCATATTCAATCATTCATGTACTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAATATACCTAAAAAGGCACCCTTTCACGGTCCTAATGCCAACCCAAAAATAATGAATACTGCATAAGTAATATTAATATTCATTATTCCTTTGAAAACAAATCTTTCTAAGATTGCAGCAATTATATAAATAGCTAAAAGTAAAGCAAATAAAGAAATTTCAAAAACTGTTAATTTATGTTGTTTTGTTTTTTTAATACGTCTAATTTTTTGAATAAATTCAAACATGTTTCTCCTAAAATAAACCCTCAAAGGGTTTTAAATTTTGATAAATTATATTTTTAAATACGAGTTTATATATACGAGAACAGTTTTTTAAATCATAACTATTAAATTTTAACTATTTTTTAAAAAAATAAACAAAAATAAAAATTTCGGTTCTTTGTCCGAAATTTTTAAGCCTTATTTCAACACTAATTCTGTCAAAGTTTTTGTCATTGCAAACATTGGTTTTTCATCAATATCGTTAGTTCCTGCAACATCTAAGTGAATATATTCAATTCCTTTTGTAAATTCTTTTAAGAACATTGCTGCTGAACAAGAACCTGCATGTCCGGTATAGTCTGTATTCTTCAAATCTGCAACAATTGAACCCTTCATAAATTCTTCATAGTCCGAGTCAAAAGGCATTCTTCATATCAACTCATGTTGAATGGTTGCTGCATCACTAATTTCTTTTCATGCTTTTTCAGTTGTTGATCAAATGCCTGTATATGTGTCTCCTAGAGCTGCAACCATTGCGCCTGTTAATGTAGCAACATCAATTAATCTTGTTGCTTTTAATACATCAGCTCCATAGTACAAACCATCAGCCATAACTAATCTACCTTCAGCGTCTGTATTATTTATTTCAACAGTCTTTCCGCTCATTGAAGTCCAAACTGAGTCAGGTAATGATGCATCTCCATTAACTCTATTGTCAGTAATACACATAATTGCTGATACATTTGTTTTTGGTTTTAATTGAGCAACAGATTTTAAAACAGATGCAACTATTGCAGAACCTGACATGTCATATTTCATTCCTAACATATTACGGCCTGTTTTAATGTTGTAACCGCCAGAGTCAAAGGTAATTCCTTTACCAATTAAAACTGTTTTTTGATTTGAATCAGGATTTCCTTTATATTCAATTATTACAACTCTAGGTTCATAAACTGAACCTCTATTCACAGATAGTAATAATCCCATACCCAATTTTTCAATTTCTTTCTTTTCAAGAACTTTAACTGATAAGTTTTTATATTCTTTAAAGTCTTCAACAACTATTTTAGCTAATTGTTCAGAATTTAATTCATTAGGAGGGGTAATACCCAAATTTCTTGCTCAGTTTTGAGATTCAATTAATATTTTTGCTTTATTGAATGAATCAATTACTGATTGAGACGCTTGTTCTATAAACAATTCTATGTTGTTTGGATTTTCTTTGTATGTAAATGTCTTTTTATTGTATAACTTTGCAGCTGAGAAATTTATTCCTTTTGTGAATGCATCAACTACATCATTAATTGTTAAATTTTCTGTTACAAAAGAAGAAAGATCAATTTGATAATCGTAGGCAGCATTCAATGCTAAATCTTGTGCCAATGAATATAAAGAATTGTATTGTAATTCTTGCTTTTTGCCTAGATAAATATACGAAACTTTTTTATCAATAAAATCTGTTATTGCTTCTGTTTCTTTAACTAAAAATTCTGGCAAAATTTCATCTTCAAATATTGCTTTCAATAACAAATGATCATTGCGTTTTGTTGATAATTGTTTAATTAATTGCATACTATTCTCCTTATTATTTTTGATTTGCTGCTAATTCAACAAAGGTAGAAACTAAAATTCCTAGTCCTACTCCTTTATTGTCAGCGGTTCCTGCAATATCGCAATGAATATAGTCAACATTATTTGTAAAATGTTTTAAGAACATTGCTGCTGTATTACAATCTGATTTTTCACTGTTGTTGTAATTATTAAGATCAGCTACTTTAGATTCTTTATTTGGTTTATCATAATCATCATGTAGGGGCATTCTTCAAACCTTTTCATGAGCAATTTTTGCCGCTTTGTCGAAATCTTCTCATCTTTGTTCAGAAGTTGATCAAATTCCTGAATACGTCTTTCCTAATGCTCTTTCCATCGAACCGGTTAAAGTTGCAGCATCAATCAATAATGAAGCATTCAATTTCGAGGCACCGTAGTATAAACCATCAGCCAACACTAATCTTCCTTCAGCATCAGTATCAGTAATTTCAACTGTTAAACCACTCATTGATTTGATTACAGATTCAGGCATTGTTGGGTGTGTATCTATTGCGTTATCTGTAATCATCATTATTGCTGCTACGTTTGCTTTTAATTTTAGTTGAGCAATTGCTTTAACTGCATATGCAACAATAACAGAACCAGACATGTCAAATTTCATGCCATCCATGTAGTATCCTTTGGTGTTATATCCTCCGGTATCAAATGTAATTCCTTTTCCTACATAAACATATTTTTCTTTACTTTCAGGATTGCCATTATATTCAGCAATTAAAACTCTTGGTTCATAAGAAGAACCCGAATTTACTGCCAACATAAGTCCCATGTTATTTTTCTTGATGTTATCACGATCTAAAACTTTTATTGATAGTCCAGGAATATTTTCTAAATCTTCTTTTACTACCTTAGCGATATATTCACTAGTTGCAACATTAGGGGGTGTCATTTGCAAACTACGGGCTTTATTTGTTGCTTCAAGAATTATTGACAATCTTTCAACTAATTCTTCATACGATTTATCAGCATAAAACAATGTTATTGTCTTTTCTTGTTTGTTTTCTTTGGTTTTTGCTGAATAAAGATTTGCATTTTTAAATGCTAATTTAGATACAAATGCTCTAAAAACTTCTTCTGTTGACACAACATTTTCTTTAACAAAAGAAGCAATATTAATTTGATAATTTCTTCTATTTGAACTTAGAATTTTGTCAACTACTTTTAAAAAACTAAAGTAGTTATCTACAGTTTTTGGTATGTAAATAAATGCTTCATTTTTTTCAAAATCTTCTGTAATATGATTATCTTTCTTGGCAATATTTAAACTAGCGTTTGAGCCTTCATAAACTGCTGTTAAAAGCATATCTTTATTACGTTTAGTTTCATAATAAATAGAATTCATTTTATTCCTTTCTAATTTTTAAATAAATATCTTTTAGATAATTAAATTATAATGATTAATATTTTTTAGAATGTAAAAACATAAAATAAAGAGAAAATTTTTAGTATTTTTAATTAAATTAAAAATAATAAAAAATTAGCCTTGCTAAAAGCTAATTTTATTATTTAATAGTTTTAAGTTGATTAATAACTAAATCTATGCTAAATAAGATTGCGTCGCATTGTTGATCATTTAGTTTTGCAATTTCTTCATCTTTTATCATTTTTTCATATTGGTTCATAATTTCACCGGCAGATGGTATCTTTTTTGCTATATCAAGTAATTGCTTTATTTTTGATTTAACTTGTTGTTGCTTTGATGTCAATTTTGAACACGATATTGCTACTAGTGGTAATGAAATTAAAGAACTTGCTCCTAAAATAGACAAAATTATTTTTTTACTCTTCTTCATAGAATATCCTTTATCTTTGTTTAATGAATTGTTAGATATGATAATTATAAATATAAATTTAATTATTTTTTAGTAGATCAATCGATTTCATCTTTTGATAATGATTTAAGTTTAAAATTTATTTTTTTAAAAACGTCTGAGCCAAAAAACCCTTTATATGCAGCAAGAGGTGAAGGATGTGGCGCATAAACTATTATTTGTTTAGACATATCAATGTTTTTTATAAAATCTCTGGCGTTATTTCCTAAAATTAAATAAATAATATTGCTATAACACGAATTAATTTCTGTTAATAAATTTTGATTGAATAGTTCTCAACCTAAATTCTTATGTGCCAATGTCTTTCCTTCAATGTTTGTTAAAATTGAATTTAAAAGTAAAACGCCTTGGTTTTTTCAATAATTTAGTGAAAAAGTTTCAAATGTTGAATTTGGATATTCATTTTTTATTTCAATAAATATATTCTTTAAAGATGCCGGGCATTTTGTTGCATTGCTTGAAAAACACATACCATCTGCATCACCTTTGTTAGGATAAGGATCTTGGCCAATAATAATAACCTTTAGGTTGTCAAAATCAAAATTTTTAAATGCAAAAAATACTTTATTTGGCTTTGGCGTTACATTAGGCAACATAGTTAATTTTTTTAAGACTTCAAAATATGGTTTTGAAGTTTCTTTTTTTAAAAATTCTAAAAAAGCAAATTTTGACATCTTTAAACTCTTACTCTTTTTTTCCCTTCAAATTGATATTCTATTAAGTACAAATATCCATCATTAAATTTTATTTTCAATGGGGTACTAATTTTTGTTGTTGAAGCCCGGAAGATTTTTAATCTTTTATTTTGAAAAATTAAATAAGCACCTGGTTCATCATTAAAAGCTCTGATTTTGTTTACAACACTTGAAAGATTTTCGCTTTGATCAATCCTTTCCATACCATTTAATATCTTAGGAGCAAATGTTACTTTAGATTCATCCTGAGCCACTCGATTATATTGGCCGTTGTAAATTTCAAACAATCAATTACCAAGATTTTTTGAGGCCACATCTGCTAATTTATCAAATACTTCAATTGCTGTATCTGCAGAATCAATTTTCACTTTCGAGGATTCTAGGATATCTCCTGCATCCATTTGTTTTACCATTTCAATTAATGTTATTCCTGTTTCAATATCACCATTGAGTAATGCATATTGCACAGGCGCAGCTCCACGATATTTTTCAAGCAAACTTCCGTGAACGTTCAAGGGCATTTTTTTAGGTAATTTTAATATTGAATCAGGTATAAATTGTCCAAATGAAGCTGTGATAAAAAAGTCAAAATCCATTGCACTAAGTTCGGGCAAAATTTCTTTTATTTTTTGAGGTTGAAAAACTTTTATATCATACTGTTTGGCAAGTTTAAAAATTTCTGTTTCAATTACATTTTTGTTTCGATCTAGTTTCCTATTTGGCTGAGAAATAAATGCCAAAACATTGAACCTTTCGTCATTTAAAAGAGGTTCAAAAGTTTTGGCGGAAAATTGTCCAGTTCCCGCTAGAACTAAATTAATTTTGTTATTTATATTTTTCATATCAAAATTATAAAATAAATTATTTTTTATAAACTTATTATCTTGTATTTCAAGCAAATGTTTTTATTGACAATAAACTCAATATTACAATTCAAATTCAAGGCAATATGAAGTTCAATAAATTTTCTGTTTTTGAACCTATTTTTGTTAAACTATCATTGCTTAAAACGTTATTTGAAAATTGAATGTAACTACTGTCTAAAAATTTAAAATAGTTTGCAAATTCATTATTCTTTAACAATTGATCTAAACCATTTTGATTGAATGCTTCAGCAATGGTTGAATATTTTAAAATTACAGGTTGATAAACATTTAAAATATTTAGTGTTTTATATTCGTTGTTAATATTAAATATATTATATTGACTATATTTTATTCCAATTGGTGTATTGGCTGGCAATGCATTATTATTTGCTAGAAATAAATCTTTTTGTTGATTCAAGAATTTCAAAATTGTTAACCTTGCAATTAGATCTTTGCTATTTGCTTCTTTCAATAAATTTGCATATTTTGTAACACCTTCAATGTATTTTTGAAGTTCTTCAATTGTATAGATTTTATTGCTTAAATCAGCACTTGAAAAAGTCTTTAATAAATCTATATTTTTTATTTCATTTCCGATGTTAAGAGTTGCCTTTATTGAAGCAATATTATACTCATTTGATAAGGATGCTTGTCCTTGAAAAGAAATTGTGTTTTGAATTATTGCACTCTTAAAGGGCGTTAAATAAGAGACATATCACATTCCTTTTATATTTGAAATTTGAGAAATAGGCAACACTTGGCCCGATAAGAACATTGTGATTATTAATAGCGAGCAACCGATTGCTTGAATAGTTAATACTGATTTTGAAACGCTTATAATGAATAGCCCAACAGATATACTTACAAAAATTATAATGACAAAACTAAATATATAACCTGGCCAAAATATCCTTGAAAACATCTCTTTAATACTACTTCCGTATAAAGTTATATTTAGACTTGCTTCTCCAATTTTTATATTATTAATTGTTCCTAAAGAACGACCGGTTTTAAAATATTCGCAAAAAAACATTAAGGCAACCAGCATTGTTCAAAGGCCAGAACAAATCATTATAATCAAATAAAATATTGCTGTATATGCCAAGAACGATTTAGGCCTAATGCTAGAGGCACCAATTCTTTTTAAAATTGTGGATTTTTTAAATTCAAATATTGCTGTTGGCAGTGAAATACAGGCTATTGCCACCGGACCAATGGTAATTGTACTTCCTAAAATCATTTCATAACCAAATATCGTTCCTAAAATAGCAACAAAAATAATTGGATAGCCGAATGCAAAAAATGGTCCTACAAATGATTTTCAAAAATGTTTATTAATAATTGCTAATATTCCTAAAAACTTATTTTTGTTAGCCTCGGTATTATTGTTTTTTTTAATAATTTCTTTATTTTTCATAATTTTAAATATATTCCTTAAGACATAGGGATATTGATCCGTATTTTTCAATTATTTCACTTTTCAAAGCATCAAGGACAATTCTACCTTTATTCATAATAATAATTCTATCTGCCAGAATTTCAATTTCATTTATATCGTGAGATATTAAAATCAAAGTACAATCAATTTCTTTGATATAGTCATTCAAAAATGATACCAATCTTGTTTTTATTTTTATGTCAAGACCAGTGCTTAATTCATCTAATATTAACAACTTTGGTTTATTAATTATTGCAAGCAATGCATTAAGTCTTTGATGTTGGCCCCCGCTTAAGGAAGAAGCTTTATTATTTATAAATTCATCAACCCCAAAAATCTTTATAAGTTTTTGTAATTCTTCCGGTGAACTTTTATTGCCATAGATTTTGTTCATTGAATAGATGACATCATTAACCGATAAACCTTGAGGATATGAAGAATCCTGAAATTGAATTCCTATGTTTGTTTGTGCACTTTCATTATGATTTGATCCATCATTAAAATAATATTCAATATTGCCTGAGGTAGGTTTTAAAATTCCTATAAGCATTTCAACTGTTGTAGTTTTTCCGGCTCCATTTCCACCTAATAGAGCAATGTTTTGATTCTTATAAATTTCTAGATTTAAATTATCTACAACTTTTATTTTTTCTTTTTTTATTTTGAATTCTTTTGTCAAATTTTTAATTTTTACCAACTTTTTTAAATTGTATTGGCGCATTTCAATTGAATTCGCTTTAATTGTGTTTTTTTCATGATCTTCTAAATTACTTAAAAAATTGTCTATTATTTCTAGACTTTTATTTTTACAAACTTTTTCATTTTCCACTATTTTAACTCCTTATTTTATATATTTCCCTTTTTGCCTAAATTATATCAATAACTTAAAGAATGAAAACAAAAACTCGTAATCAAAGAATTACGAGTCAATGTTTTTATGCTTTTTTGTTTTGCTAAAAATTTTTGGTTCTGTATGTTTGAATATTCGTTTAAAATTGGGTATATGTTTAACAACTATTATTAGCGAAGCCAATATTAAAATAATTACATGCGCGGGATATACAACATTTTGTTGCATATAACCGAATATTGTTCCTGCATAAAATATATAAATTAAACCTAAATAAGGAATTATTCCACAGCTTATTGATGAAGCAAGTGAAACATATTTTGTTGAAAAAACTAGTATTACATATATTGCTACAAAAATTAAAAATAATAGAAAATTATATGCAAGCATTATTCCAAAAAAGCAGGCTACTCCTTTGCCACCTTTAAATTTAAAATAAACTGGAAAGACGTGCCCAATTACTGCGCCAAGCCCTGCAAACATTGGAAAAATATATTCAAGATTTTTTAGATATTTTTTAATTAAAAATGCAATGATTGTTGCCAAAAAGGCCTTAAAAATGTCAAAAGCAAAAACTAATGCTGCAAATTTTATTCCGAAATTTCTTAATGCGTTTGTTGCCCCAGGATTTTGCGAGCCAATTTTTCTTATATCATTCCCTCTTAATTTAGTTATTATTATTGCCAAATTAAAAGATCCAATTAAATATCCTAATATTAAAAATATTATATTTAATCACACATATTCACCATAAAACATAAATAAATGATACACACAAGAGAAATTATTTTATAAAAACTTACAAAAAAATAGCAAAACTCTATTTTGTTTGCTATTTTTCGGTCTTTGTTTCAATGTTAATTATATTGCTTGCAATAACATCACTACCAACTAAAATATCATAATGAGCAGATATCTTGTTTCCTTCAATAAAAACATCTTTTAAATAAAAATCTAGAGAAAATTCTTGAATTTTATTGCTTACTCTTTTGTTTTTTTGTAACTCGATATATGAACCAGAATATTTTATTTTTAAAGTATCGCTATTGACTAATAAATCACATTCCTGATTATTATTATCAATAAAATTTATATGAATAAATTCATCAACAACTTCTTCGGTATATGGAACAAATTCTGTTTCTATCGTTTTAGAGGGTTCATTATTGAATTCGTTTTTTGTAATTAAACTATATTTTAATAATTCCATTTAATCTCTTAAAAACTAATTCAGCCCCAAAAAGATAGATTGCCTTTGCAAGTTCAGGGCCATGTTCCTCAAATGTTGTTGCCTTTCTAATTGGCATGAACAAATCTCTTCCCTTAACATTCAAGATGGTTTTTGTTTCATCAATTGCTTTTTGTATATTTTCTATATTGAATTCTCTTGAAATTAGCTCTTTATAAAAAACTTTTACAACATCTAATCTTTCTAACTCTAAGTCGCAATTTTCTTTTGGGTTTAAATAAATTTCTAAATCTTTCTTTAATGTTGAAATCGTTGGACTTGATTGTTTATAAGTTTCAACAAATAATTCATTTCATTCATGTTCTTTTGGAGAGTTTATTTTTTTAATTAACTCTTCATTAGAAATATTTTTCATATATTGCTTTGCAAATCAATCCATTTTTATAATGTCAAATTTAGATGGTGATTTGCTCAATCTTTCTGGATCAAATTTTGCAATTAATTCTTCTCTATTCATTAATTCTTGCTTATCTCCGGAAGTTCAACCTAATAAGGCTAAAAAATTCATTATTGCTGCTGCGGGATAGCCTTCATTTTTATAGTCTTCAATAAATTGCTTTAAAGTTTTATCTCTTTTTGAAAGCTTTTTTCCTTCCATATTCGTAATAATAGTTAAATGTCCAAATAATGGGGGTTCTCATCCAAATGCATCGTATATTGCTAATTGCTTTGGTGTGTTTGTGATGTGTTCTTCACCTCTTAGTACATGTGAAATTTCCATTTGGTGGTCATCAACAACAACTGCAAAATTATATGTTGGATAACCATCACTTTTCATAATAACAAAATCTCCTATATCATCACTATTAACAGAGATTTTGCCTCTTACTAAGTCATTCCAAGCATAATCTTTATTTATTGGCAATTTTAATCTTATTGAATATTCTTTGGCTTCTTCTCTTCTTTGCTTTTCTTCATTAGAAATTTGCAATCAATTTGGATTATAACGAAATGATGCAACGCCCGCTTCCTCTGATTGTTTATGTTGCATTGCTAATTCTTCCTCTGAATCGTATGCTTTATAAGCGTGTCCATTTTGAATTAGTTCTTCAACAAGTTTTTGATAAACTGGTAATTTTTCGCTTTGACGATATGCACCATATTTTGAATTAGGTTTTAAAGGACTTTCGTCAGGAATGATACCAAGTCACGCTAAATTATTTAATTGGCTTTCTTCACCTCCAACAACGTTTCTAGCAACATCAGTATCTTCTAGCCTAAAAACAAAAGTGCCATTGAAATGTTTTGCAAATAAATAGTTAAATAATGCAGTTCTTGCACCACCAATGTGCAAATACCCAGTTGGGCTCGGTGCATATCTTGTTCTTATAGTTTTCATAATCTACCTTCTTTTATGACTTTTCAAAAATAGCAGTTATGGGTTCTGAAGCGTTGCTTCAAAACGCATCCTTATTTCCCTTCATAATTCCTTTATAAAAAGGTCAAGGTGCTTCCTCAATAGAAGTATTAACATTTCTATTTTTTCCCTTAGCTAACAATGATTCAATACTTGGATCTAGGAAATCTGATTTTCAATTAGGAGCTGCTTCATTTTGTTTTAAAGTTCAATATAAAACTAAAAATAATGCTCTTGGATTATCTCGATAAGGTCAATATCTGGAATCATTTTTTGTAGCAGGAAAATAATTAATTAATTCCTCTCATGTAAAAGAGAAAACTCTAAAATCGTAATTTGGAGAAATATTATTAAAATCTAAAAATCTGCTAAAAAATTCTCTATAAACTCTTTGCACTGGTTTTCCGTCAATTACTTCGAGTTCATTATTCAATACTTCTTTCAATCTTGGATATCCATTTTCTTTGTCATAAATGTAGTTTCCGTTGGCATCTCTATCGAATTCTATTTCACCATTTTCCAATTTTTTACCCTTAAATTTTAATAAATTTCCATCGGCATCTCTAAAGTAAATTGGATTTCCTTTTTCATCTTTTAAATTTTTTGTTAATTTGTACAAGAAATCTCTTGGCGATATCGGGTTACTTACTGAATAATTATTTTTATTAAAATCAAGTGCAGAACCCTTAACGTGATAACCATATTTTTCAAATGATTCTTTCGCTTTTGAATTGAGAGTTATGGTTGCTGTTTTATTACAGCTTGCCGCAACTAGTGGCAATGCAAACACAGGAGCAACACTTCCTATACAAAGTAAAAATTTTGTCATAATTGCTTGTTTTTTCATGTTTAAAATTATACAAGTTTTTTTAATATATTAATAACCATAAAAATAAAGTTTCTCTAACTTTTCGCCATTTCAAAAAAAATAAACTTTTTCAATCAATAATTTAAATATGTGAATAAATAAACAAGATAGCGTTAAAGATTGTGCAATTTATACATTGCAAGCAATGATTTGCCACTTTTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAATACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAAAAAATTTCTGATTTTGTGGGGTTTGATACTAACATTTACAAAGTAGATTTTTTAAGTTTTATTTCAAATTTGAATGATGTTTCATTTCCATTTGTCACAATATTGAAAAATGATAATTTCTTGCATTATACAATTGTTAAAAGAATTGATAAAGCTTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAGATAAATTCATAAATGTTGTAGTTATATTAAGCAAAAAATTTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAACACAAAACACAAAGTTAACAATAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTATATTAAATATGTTTTAAGCGTTTTATGATCGGAAAACGCTAATAATAGATTGCTGTCAATATTAATTATTTTTAGTTGAATTGCAATATTAAAGGCAATTACTTGATTATTTAAAAAGATTATTATTAATAAACTAACTTTTTATTATGAAAAAAAGTTATATTTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAAAAAGACCTTCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTTCAACCATTGCAGAATATAAGGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACACTGCTTTTAGGTCTGTTTAATTATAGAATATTATTAATTTGCTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATGTTCAATAATTATTTTTTAAATTATGAATATAGGACGAACGATTTAAATAATTCGAACTCTAAATTATTTAAATCGTTATTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGAGATTTTATTCAATTAACCATTATATTTACTGGCGCTTGATTATTTATAAAAAATAAAGATAATTTTACGAATATTACATTATATGGATCAATATCAATTTTTTTGAATTCCCCTATTGTAAATTTAGTCTTCCTGATTTCAAATTCTTCAATAAATAAACAAGGAATAATAAGAATAAAATATTTATTAGAAATCACGCAAAACAAGGATGGAAATATATCATTCAAACCCCGGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAAAAATCTTAATTTAACAATTAAAAACAATCTAGAAATATCAGGGAAAAACGGAGTTGGTAAAAGTTTAATTTTGAATGCTATATTTAAAGTTTATTGATTAAATCAAGGCCACATTTTTGCCAATAATATAAATTTAAAGCATATTAGTCAAAAGTCATGATTAGATGTCTGCTTTTTAAATAAAAACAATGAATATTTTTGCTATGAAAATCTAATGGATTGCATATGCAAGAAAGACAATATAAAGGAAAATATTTTTCTTAAAAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGGGGAAATAATCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAATATTACAAATGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCTATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTCGCTTTAAAAAAATAAATATTTTTTTGTTTTTATTTAGTTTAAAAAAGAATATTATCTTTATAATTAATAAAATATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAATCAAAAATAGAATTTGGTGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCTAAAAAGATGAAAAAGTTAAATAACTTACACAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCGTGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGAATTGCTCACCTATTTGGTTTTGATCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTAGATAATATAATGAATAAATTAAACATAAAGAGAATATAAAAATTGGAGAAAAATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGTTAAATTTTAACCTATCAATAATTACGGATAAACCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTATCAAATCATTTTTATTGATACTCCAGGAATTCACAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAGCAATCGGAACGGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCAGTCATTTCAAAAATTGATTTATTAAATACAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAAGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTTAGATTTGATTAATGAAATTAAAACCTATTGCTACGAAGATGAGCCATTGTACCCTGATGATCAAATTGGGGATATAACATTGAGGTTTATGGCAAAGGAAATAATTAGAGAAACTGCTATAAACAATTTAAAATTTGAATTGCCACACTCAATTGCGGTTGAGATCGAACAATTTGATGATAAAAACGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATTGGAAAAGACGGTCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAATTTTTAATCACAAAGTCTTTTTAAATATTAAAGTAAAAATTAGCGAAGATTGAGTAGATAATGAATTCAAAATAAAACAGTTAGGATATTAAGGTGAAACGATTAACTACACATAGAACGAGAGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTTTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAGAAAATAATTACCAAATTAAAACAAAATTATTCATATCACGCCTTTACAAATCTAGATGAGTTTACTAGAGCGATAAATAACGAAAAAGCCGTTGCTGGAGTTGGAAGTGATTATCAAATTGCCCAACTAATTATTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTGTTTAATGATTCAAAATTTAATGATTCATCCCAAATAAAGAAGCTTTATCCAGAAATTGTTAATAAACATTTTGATCTATTTGAAAAATTTATCATTAAAAAAATAAAACAAATAAATCCAAAAAACATCAAGCAAACTGATTGACAAAATATTAGTCACTCCAAATTCTTGCCTTTTATTTATTATGACGAACATAACAATATTTTGGGTTTTGAAATTGATCAAAAAGAGGGAATTGACCACTTTTATGAATTTTTAATTCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAAGTCCAAATAAATTTACTGAAAGAAGCAATTTAAAAAATAATGAAAACTTTAACGATTTAGAAAATTTAGATTCTTGAAAAGATATCATTAAGACGCTTGTTTCTAAATACAAAAACCCTAGAGTATATTGAACAAATTGATTTCAAGATAATGCTATGATTGGTCAATTTTATGGTTATGAATCTCTTAACAAATTAAAATATTTTTCTAAGCAAAACGAATGAGCAGATATAAATCACGAAAATTTTAAAGAGATTATTAATGATTGAGTTTCTTTAGTTAAGGATGCAACAGGAGAAAGTATAAAAAATTCTAAAACAAATAAATTAGCTACTGATGGACAAGAGTTGGTTAGTTCAATAATAGAGCCGATTAATGGCAAGGCAGATGTTTCAATTATGTATAACGGAGATGCCTTAGATGCTTATTATGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCACATATTGGTTTTAAAAGGCCAAAAAATACTTATATGAATATTGATGCTTGAATAATTTCAAGAGAAACATCTGAAAATGATGCTAACAATTTATTAAAAATATTATATGAAAATGTTATAAAGGGAGCAGAATATTCACTCCAAGATATTGAAATAAAATATTTAAAAGAAGTGTTGAATCTAATATCAGAAGGCAACAAATTAAAAAAAGATGAAATTGTTAATGAGCTATTTGCAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTACAGATTATTTTGACTATTTCAAAGAAGCATTTGAAACAATGAATTTACCCATTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTTTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTGGAGATATTCAATCCAGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAATATTATTTAATTTCATGTTAGGAGAAAAATGGAAAATAAAATTAAAACACCAAACAAAAAAGAAGAACCAATAATTCAATTAGTAGACGTGGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTATTAGGTCCGTCAGGTTCAGGAAAAACAACCATATTGAGAATTATTGGCGGTTTTGAATGAGTTACCAGAGGTGAGGTTAAATTTTTTGGCAAAGATATCAAAGACTTAAGTCCACATAAAAGAGATGTTTCCACAATTTTTCAAGATTATGCATTATTCCCTCACTTAAATGTAGAAAACAACATTAAATATGGTCTTAGATTAAAGAGAATACCAAAAGATGATATCCCTAAAAGCTTTTACAATAAATTAAAATATCAACAGAAAAAATGAACAAAAAAAGCTTCTATCGAAATGAAAAAGCTTGATAAAACCATGGCACAATATGAAAAAATTCTTGAAAACCAAAAACTTCCCGAAAAGAAACGGAACAAATACCAATCTTGAATTGATGACATGGATTTCAAATACTCATATTGAGAAAATTTTAAAGATTTAAAAACTGAGAATTATGAAAAAAGATATCTAACAAGAAAAATAACAAATAACGAAATGAACCAAGAAGTATCAGAAATCATGGATCTTGTTGGTTTAAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGAGGTATGAAACAACGTGTTGCGCTTGCTCGCTCGCTTGTTATTGAACCCGAAATTCTTCTACTAGATGAACCATTAAGTGCTTTAGATGCTAAAATTAGACAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTCATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATCAGAGATGGTAAGGTTGAACAATTTGCTTCGCCAAAAGATTTATACGATTATCCAGTAAATAAATGAGTAGCAAATTTTATAGGCGAATCTAATTTCTTTGATGCTGTATTTATTGATGAAAATAAAGTTAAATTTATGGGCAAAATTTTCGATACTATTCACAAAGAATTTGAACCAGGAGAAAACCTTGATGCATTAATTAGACCCGAAGATGTTTATGTTACCAAAGTAAAAGCCAAATCTAAATTTATTGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTGGGTAAGGAAGTATATTTAGATTGATACAAAGATTCAATTCATTTGATGAAAAAGGATATTAAATTTGCCAAGGAACTGGCAGAACAAAACGAGATTAACAATGGAAAAACAACTGAAATCAACTTCTTATAAAGAAATAGACAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTGGGTGCAAACAAATTGCAACTTTCTTTGCTAATTCCTTATTTAATATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGTCATCTTGCAAACTGGGAAATAGCAGGTAAATCATCAACTTGAATGATTATTCTTAGATCAATCGGAACCGGAATATGTGCAGCGTTTTTTGCATTAATTATTGGTTTTCCGTATGCCTATATTATTGCAAGAAGCAAATCAATGATATTTAGAACCTTAGGATTAACGTTAATTCTTAGCCCTCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTGTTTTCGGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCCAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACGCAAACTGTTTTTAAAGTTATATTACCCTACTGTATGAAGGCTATATTGAGCGGTTTTGGAGTTGTCCTTATGATGTCAGCAACATCAATAATTCTTTCAGATAAATTGTTACCAAATGGTAGTCAAAAACAACTTATAGGAAATCTTATAAATCAGTTTGCTAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTGGCTGTTTTGTTAACAATATATGGAATTATTTATGGAATACCTTATTTAATTTCAAAAAGAAAGCAGGGAGGAATATATGAATAAATTTAAAAAATTTTTGAAATATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATTATTTTTGGAACAATATATAGCTTCAACGCACCTTCTGACAAGGGAATATTTTCTGTAACCTCATGAAATAGAACTTCTTATGAAGCGTATGTGGAACTTTTTTCAAAATCACATTGACTTGCATTTGTTAATTCTTTTTTACTAGGAATTTCAACATCTATTTTTGTTATATTACTTTCATTGATGACAGTTTTTGCACTTTGAAGGCATAAAAACAAAACAGTTAGAGCATATGTGCAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACTGGTTTAACGCTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGAGCAATAATTTCGCATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACCTGATTTAAAACTTATTTTGTTTATATGTTAGGTTCAATAGGATTTACATTTGTAATCACAATGACACTTTCTTTTGACGACTTCATAATTACTAGAATTGTTTCAAATACAGAAACTTTGGGTACTCAATTATATGAAGGCCAATTTCAAGCATGATCGTTAGCTATAGGTGGAATATCATTACTAGTAGTTTTAATGGCCAATACAATATATGTAGCTATTAAATCTTCACAATTTTCACATAAAAAAATATCATCAATCAATATTTCTTCAATAAAAAAATAATATAGGAGGTAATTTTATAAATTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCTTTATTCCTTGCTTTTTTAGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCTCCAACCATTATTAATAAATTAAAATCAAAATACAATTTCAAAGAATTTAAAGAAATTAGTGAGTTTACTCAAGCATTATTACAAGATAAGGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGGAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGAAAAGAAAGACAATTTCTATATACCCCAAATGTTAGAGAGCATATTTTAAGATTTGATGCTCTGATTTTAAATATTTTAGAATCTGATAAATCTAATAAATATAAAAACTTCGAAATAAAGAAAAATAGTGAAGGCAAACCTATAGCATTTAGAGACAAATTAAACAATAACAGCGAAGAAGTTTGGGATCATTTTTATGATTATATCATTCCTTATTTTACCCAAGATAAAGGTATAGCTTACAATATAAATCCAAAGACTAGACCAAATTTAAATATAAACAACGCTATAGAAAAACTAAATAAAAACGACAAGATTTTTTCTTGAAATGAAATCTTACAAATTCTAAGTAAAAATAATTACACACATTTTGGTTGAACTCATGCTTATTTAGATAACATGATGATAGGAGCGTTTAACTCTGGAGAAAATTGAGAAGAAGTTTTTACTAAAAAAATCGGTGACAACAAAATTTTTGATTTTAATGAATCAAACTATAAATTAGCTATTGATTCATTTATAAATTTTGTTAAAAATTCTACCGGAAACGATATAAAAAACAATAAGAAGCATTACTTTAGTGGCGATGGTCTTGAATTATTAAATCATCTAATTGAACCAAAAACTTCTAGATCTGATGCCTCAATAATGTACAACGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCTAAAAACACATATATGCTAATGGATAATTGAATAATTTCAAAAAGCCTTTCAAAAAATGAAACAACAACCTTTTTAAAAGCCATAAGAGACCATGTTATCAATATTGATAAACACAAAAATAATATTCAAAATATTGAAAAAATATTTTTTAATCAATTTAAAAACAACATGACAAAACAATTAAAACAATCTGCTGAAGCTTCTTTTTTTGAAACCATACATAATATTGACAAAACTTTAAAAAATAAGTTAATTCAAGAATTTCTAAAAAATAAAGAATTTTTCAATATAAATGAAAAATCAACTAGCTTCGAAGAATGAAATAGCAAAAAGTTTAGAGAATTTTTAAAAAATCCTACTATTCAAGAAAATTATGAGTGGTTTTTAATTTGAAGGAACAATTACCAAGACCATAATGAAGCTTTCTTATTTGATGATCTTATGTCTGATGCTTTTGCAAATAGTGAAATAGGAAGCATATCTAATTTTGATTATGTATCATATACTCCAACTGAAAAAATAATTTATGATTTTATTAAAAAATGATATTTTGGTTCAAATAAGGTAGCAATATCAATATTCGATCAACCCGAATCAAATAACAATTACCAAGTTTTTTCATATCCAATTATTGACAATAATTTAAGAACAAAAATAATTACTTATTATTATGAAAAAACAAAGTCCTAAAATTAAAAGATCTATTCATAAATGTTTAATAAGATTTACAAAATTTAAATAATATATAATTTTTAGTATAAATTAAAAATTGAAAGGAAAAAACGTCATGAACATAAAGCAATTATATAAGCAAATAAGCCAGTTGCAAGAAAATGTTGAACTTACAATTGAAGGTTGAATTATTTCAAACCGTGGAAACGATAAAATTCGTTTTATTACAATTAATGATGGTAGTACAATTGCTAACATGCAATTAGTTGTTAAGGGAGAACTAGCATCAAAGAAAGAAATCTCTGAAATTGCCATGGGTTCAGCTGTTAGTGTAATAGGTAAATTACACTTAACACCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCTCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCAGGAGAAACTCTACTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTCTAGGGGTAACCGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGGCCAACATTTAGAGCCGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGAATGATGGAACCAGAAGTGGCCTTTTGTGATTTAAAAGGTATCATTGAAATTACAGACGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATTTTTGGATTCATTACAAAACAATAAATTAATCGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGATGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTTGAAGAAAAAGATATCTTTTTCGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCCGAAAACATCATAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTATTAGTACCAGGTATTGGCGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATATCAAAAGCTTGTTGATAGAATCAATGAACTAAAAATCACTCAAGAAACATTACAATGATACTTAGATTTAAGAAGATTTGGTTACGCCCCAAGTAGTGGTTTTGGTTTGGGCTTTGAAAGACTTGTAATGTATGTTACTGGCACAAGCAACATAAGAGACGTTATTCCTTTTCCTCGTGTTGCTGGCTCAATTAAAATGTAGGTTTCTATGCAAGAAAATAATTTAACAATATTAATTCCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTGTATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCCAAAACCTTTTTCTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATAATGTATTCATATATTGAAATTGAGTATGATTTTATTTCAAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGAATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCTAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGATGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGAAATTATGATGTCCTATTGTGAAGCGTTAAATCTTTGTTGGAATCTTGAAAAGACATAGAGTCTGAGTATTCTATGCATGAATTTACAACTCCTTTTTCAGAAAGTTATTATTATCTTGCAAAATTTTTAAATATAGCATATTGTTTCGCAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATTGATAAGTGAAAACATTGAAGAATGAAAAAATAGCAAAATTATCAATGATTTTTTAGAAAAAAACCGTATTCAACAACTTTTTGAATTAATACCAAATAGTAAAAAATGATCACTTATTTTTAAAAAAATAATATGATAAAAATAAATATTAAAGCTAATTTTTGAATAAGACTTGCTGCTACTTTGATAGATTTCTTGGTATTCATTGGTTTTTTAATTGGTTCTTCGTTTGCAATATTTAACTACAAGGAAGCAAAATTTTATAATTATTGATCTTATTATCTTTGAATAATAATGATAATTTTAGTCCAAAATATAATTTGAATAATCATTCCGATTTTTACTAAGGGCTATACTTTGGGTTTGTTTATTTGTAGACTAAAAATAATCAAACAGCAAAACAATAAATCGAAACTTAGCAAAGTTATTTTTGATAGGCAAAGGTTATTTAGTTTTATATGAATGATAATTTTTGCTTTATTTATGATTTTTATATCTCCCCAAACTTTTGAACAAGCAGCAAATATTTCTCAAACAAAACCTTTAGACCAACTTCAAAAGGCTTTTCTTTCAATTCCTAGTGTGCTTTCAGCATTAGCCACATTTTTGGAATTCTTATTTATTTTAAGCAATGCTAAGGGAGATAGAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGAGCTAAAAAATATGAAGATATTGAAGAAAACAATATTGATAATTTGATTATAAAACCCGTTGTTAGAGAATTACCAAAAATTGATTACAAATAGCAAAAAACAAATATTTGAATTTTGGTTAATTATAATTGAAATATGCACACCACAGAGGGGGTGTTGCTTTTTTATATTGCACTTTTGTGATATAATTTTATAATAAAATTTGCGCATAAAAGGGGAATAAAATGATTGATTTAAAAGATTTAAACGAACAGCAAAAAAGTGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGTGCTGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCACTAACTTTTTCTAATAAGGCTGCAAATGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCTTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTATTCAGAACTTGATATTTCACCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAACGATAATAACGAACATTTTAATGAAGACTCAGATTCACAAGAAACACAAGATGAAAAAATCAAGGCAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTTGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTGTTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCCGAAAGATGAAAAAAGAGATTTGATTATATTTTAATAGATGAATTTCAAGATACTAGCATTTTGCAATATAGAATAATGCAAAAACTTGCAAATGAACATTTAACCATTGTTGGTGATCCAGATCAAACAATTTATTCTTGAAGAAATGCTGACATAAACATTATTATGAATTTTGATAAGGATTACCCGAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATCTTAAGAGCAGCTAACAACTTAATTTCGCATAATAAACTTAGGTTAGAAAAGAAATTATTCACTGAAAACGAAGAAGGCGAAGATATTGAGTTTTATTGTGGCTTTAATGACGAAGCAGAAGCTAGATGAATAGCAACAAAAATTTCCGAATTAAAAAGAAATCGAGTTCAATTAAAAAATATTGCTATTTTATATAGAACCAATAGTTGTTCAAGAGCAATTGAAGAAGCTTTAATCAAAGAAAATACAATATATAAATTATTTGGCTCTATTAAATTTTATCAAAGAGAAGAAGTAAAAGATGCCCTAGCATATTTAAGAGTTATTCATGATGGAAGTGAAATTTCGTTATTAAGAATAATTAATAAGCCTTCTCGTAAAATAGGGAATGTTACAATTGAAAAACTTCTTTCTGCTTCTAAAAAATACAACTTAGGACTTTTTGAATATCTAGAAACTCACTTTAACCAAATTCAAAAAGAATTACATATTTCCGGCGATACATTGAAGAATTTAGCGAACTTTATCAATGAAATCAGATGAGCTAGAAGAGCAATTCAAACAAATGCAATTCACTTAACCATAAAAGAATTAATCGTTAATAAAATAAAATACTTTGATGAAATTAAAAATTCAGAAGAAGAGTATGAAAATAAAATCGACAACTTCAATAGTTTAATTGAAGCCATTGCTGAATGAGAAAAGAAAAACCCGCAAGGTTCTATTGATGAATATTTGCAAGAAATAACTCTAATAACAGATAGAGATGTTGAAGATGATGCCGCAAGTTTCGTTTCACTAATGACTGTTCACAACTCAAAAGGGCTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAACATCTTCCCACTAAAGAGGGCTATATCGATTTCTCCACAAGATGACTTTACTTTTTTAAATTCAAAAAACTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGGCGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCAGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTCTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATTTAATAGTCGGTGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGACGATGAAAGCCTAAATAGTTAAAATGTATAAATACATAGCAATATGAATCTTTCTAATAATTTTATTCTTGGCCGTATCAACCGTATTAACAATATATTACGTTATTATTAGACGAAAGAAGATAAATGCTCAAACAGGATTTTTTGTATTTAAAATAGACAGTACAAAAAACAGAATAAAAATCGAAGGCGATATAAATTCAATTAACAAAATACCTAAATATTTATGAAAGATTTTAAGTATAGATGGTCAATGGGCAAAACTAAATAACTTCTTAAATATTTTTGATTCAGGTGATAAGCGCCTATTACTAGATAATATAAAGAAAAAAAACTTCTTTAAACTAAAAACAAAGATAGCAGAAGAATCATGATCTAACTATTTTTCAAAAGTAGATATTGAGTTTAACAATGTTGTAAATGATGAAATTATTGGAACATTTACATGAAGAGACATTGACATTAAAATGGATAATGTTTTCAATAAAGAAATTCATGATTTGTCTTATATTCCAACCATTGAAAATTATGAAGGCTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATGCTTTTTTAGATCTGTTTAAAGATTTAGGAAAACAACACAATATTAATGGCTTAGAAGTTGTATATGAATGAAATGTTCTTTGCATTTTGTTTAATCATGATAAATTAGGTATTGAGAAAGCAAAATTTCTTGCACAAAAATTCATTGATATTGCAAAGCTATACAACAAACTTTATTTTAAACTTTGTTGATTTGATTCGTATATAATGAATAAAAAAACATTGGATAACGTATTTACATTCTTTGATTATTTAAAATTAGAAACATTTGAAAAAAATGCATATAACTTTCAAAAAATCCCAAGCAATATTTGAACCGAGCAAAACTTTCAAAAATTTTGTTTAACATACGAAAACATTATTTATAATCTAAGAAATAATTTTTCTATTTCGCTAAAAAATATTTCGCTAAAAAATGCAATTGGCGATAAAACCGAACTTAGAAGGTTTGGACTTCCTAAGATTTTTAATTCATTAAAATTAAACAAAGATTATGACTATTACCTAGAATTTTTAGACATAACTAGACTATTTTATAATGTCTTTTATGAAAAAATTGGTGAACTACATCCTAAAAATGCAATATTAAAAATTAGTGATTCAATATTTGAAACATTAGATATAAACGTTATTAAAAAAATCGGGGTTGAATTTCCAACATTTATTCAATCAATAAAATTATTATCAGCAGAAAGAATAAAAGACATATCCCAAAAAATTGTTAAACTACAAGGAACAAAACTCAATTTTTGTCTCGAAATTAAGGATATTGATTCAACAATAATTTCTCTGGTATCCCCTTGAGTTAAATTCATTTGAATAGATGAAGAACTAACCTCTAGATTAACCGAACCTAACATTGTTTTATATCTAAATACCTTATTAGATAACGTAAAAAATCTAGGTATTAAAGTTATTTTTGAAAAATTAGACTACAAAACATACAGGAAATTATTTTATAAACACACATCGATTATTTTATATACGACCAATTAGCGTTTTAATATAAAATTATTAAAAAATTCAAAACAAAAAAGGACAAAACATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAGTAAATAAAAAGTGGGTAGATAGCCATAAAATCCCTGATTCTAAAAGCTCTATAGGTTGCTTTGCACAATTAGATAAAAATATTACTACATTAACTAAAAAAATATTAAACACATGAAGTGAAGACGCTTCAACAATTCCAAACGACCCTATTATCAATCAATTAGTAAAATTTTACAGCTTAACCAAAAACTGAAAATTACGTTATCAAGCACAATTAAAACCAATTAACAAATTGCTTTCAAAAATTAACTCTTTAAATTCTTGAAAAGATGTTGAAAACAATTTTTTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAGCTCTTTATTTAGATGTAAATTCTACAATTCTACCAGACAAAAGTTTTTATACCGATCCTGCCAAAAAAGAAAAATTTATTAATGTTTGAAAAAATATGGTTTTAAAACTTTTAAATAAGATCGACAAAAACGAATCTAAAAATAATTTAATCATTGAAAATGCTTTAAAATGAGATGAATTAACTTCTTCATTTTTAAGAGATTCAGAATTCTATTTTGATATGGTTAAAATTTGCAACACTATTTCAACAAAAGAATTAGCCCAAAACATTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCTGATGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAGTCTTTAACCAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTTGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACGTTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACAGTTGAACACATGATAAAAAGTATGATAGATGTTTATAAAGAACAACTAACAAAAAACTCTTGGTTAAGTAAGCAAACAAAAGAAAAAGCAATAACAAAACTTAACTCAATAGGTGTTTACGTTGGCTTTCCTGAAAAAATTCAAGATCACTATAAATATTTAATAGTTGAAGAATTTGATGGATACAATGATTTATTTGAAAATGTTTTAAAATTTAACAGAATTTTAGCTGAACACATTTTATCTGAATATGGAAAAACAGAAGACAAAAACCTTTGACATATGTCGCCTGCTATGGTTAACGCCTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCTGCAATCTTACAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTCGGCGGAATTGGTGCTGTTATTGCCCATGAAATATCACACGGATTTGACAATAATGGAGCAAATTTCGATGAAAAAGGAAATATGATTAATTGATGAACAGAAGAAGATAAAAAAGCTTTTGAAGTTAAGACAAAAGAAATGATTAATTTGTTCGAAGGTAGAGAAACATCTGTTGGTAAATGTAACGGTACATTAACTGTTTCAGAAAACATAGCAGATGCTGGTGGTTTATCGTGTGCTTTAGCAGCGGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGAAGAACAAAATACCGTCCAGAATTACAATCATTATTGTTAACTATTGATTGTCACGCTCCTGCCAAATTAAGAACAAATGTTCAAATACAAAACTCTGATGATTTTTATACAACTTTCAATGTCAAAAAAGGCGACCCAATGTATTTGCCACCTGAAAAAAGAGTAAAAATTTGATAATTCTAAACACAAAAAAAGCAACCGTGCGGTTGCTTTTTTAATTAACACAAATCTTCTTTCTTTATTCCTAAAAGAACATACAGCATCACTCGTTTAATTCTGCTATTTGTATATCTTTTTGAAGTACATAAATCAACAAATTCATTATAATTTTTTGCTATTTCAATATTCTTTTTAAAAAGATTTTCCATTCCCTCGGATACTAATGCAATATTTGCCAGTTCTTCTTTAGAATAATTTTGTACCTTTTGTTTAAACTCATTATATTTATCTTCAATTTTTGAAAAATTTTTAGGAATGTTTATCGGTGTGAACATTGAAACATCTTTGCCTTCTTCTAACATTTTTCGTATCATTGTTGCCGAAGCATAATTATTATTTGCTATTAATGAATTGTGTGGAGCTGTTCTTTTTATGCAAAACGGTTTTGCTGACAAATTATTAAAAACTATGTATTTGGTATATTCAAACCCTAAAATATCTTGTGGTATATTTTCAACACCTATTAATTTTTCTAGCGACAAGAAACAACTCAAAACATATGATTTTCCTGTTTTTAAATTCATTCTTACATCTTTGTTATATTGATCAAAATTTTTCTTTATTACGTTTGCTGCGTTTAAATATTTTTTTATATCATCAGTGTCAGAAACCCCAAAAACCAAATAATCAATACCCTCTTTTATTAACAAATCTATTGATTTTTTAGCAAAAATATGAGCTGCTTGTGTAGATGCAAAAAAGTCTAATTCAACAACTTTTGTCGCTCCATATTCAAGTGCAATGTTTTTTCTAACTTCAAAAGGTAAACACGCTATTTCACCACGTTGAACATAATCGCAAGATAATGCAACAATTATTTCGGGATCTTTGAAGATTTCTTTAATTTTATTAATCAAATAAATATGCCCATTGTGAAACGGGTTAAATTCAGCTATTATTCCTATTTTCATTTATTTTTTCTTCAATTCGTCAAGGTTAATATCACAAAAATCTTCAATAGAGCTATCTTCTCAATAATTTAATTCCACAGTTTCTTGGATTTCAATGTTTTTTTGATCGCCTTCACAATTTTTGTTTGAATCAAAATCTTCGAACATCACAATATCGCTTTGTTCTTTTTCTTTTTTTATTTGTTTTTTCTTGCTTGTTTTCTTTTTATTAAACATATTTAAATTATATCATTGCAAAAAATTACTTTTGTTTTTTTAATTGTGTGATTAAAATGTTAATAAATTTTTGGAAGTGTTTGAAAATCAGGGGTCGCAAAATCATAAAGAGATTTTTCTATCACCCTAATTATACTGAATTAAAGATATTTTTTAAAAAATTCGCATTTTTTTGATGCAAATTTCATTGCAAACTTAAAAAATGCTCTACGCGCCCGCGTACGTACGGGCGCACCCTTGAATAGGGCAAAGGAAGAACCACGGAAACTACTTACCGTAGTGTGGATTTTCCTTTTTTAATATATTATTACTTGTAATAATATATTAAAATAGTATGCACAAAAGTTTGCATTATTAAGGTTTTTTAAAAAATAGCATGCTAATTGCTATCAATTCAATTTTTTATATAATTATAAATAATTATATAATAATAGCGTAGCATTAGCAAACAAGGGATTTTAAAATATATAAAAAAAGTGGTTTTTATAAAAATACCACATTTAAATTTTGGGGAATATTTAACAAGATTTTTAATTTTAAATAGTCAATATTATGTCTTGAAAACGCTGTTTTTGTATATTTTTTGAGGTAATGTCCAATAAAAGTTTCGGCATTTCCTCCATAATAATTAACATTTTCATAGCTATTAATTAAGTAATTTTTGTTATTTTTTCACAATTTTATTAGACTCTTTATTTCGACAATTTTTATAGCAGATAATTTATTTTCTCTTAAATAAATCAAATTTTCATGTAATTTATTTTCAAAATCCTCATATTTTTTGTTATTCAAAAATCATAAAAATTGAGAAAATTGATTCCCAAATGTATTATCAAATATTCTCTTATTATCCTTATTTTTCTTCTTTGAATGCCCAAAAACATCAAATAATATTTTGTGAAAATGGAACTTATCATAATACCTAATAGCATTAATTTGTTCGCTAAATTTAGTTATTATTCTTGCTCCATCTCCTGATAAATTAATAGTTTTTGCATGATAAAAATTGTTTAAAATTTCGCTGGTTTTAGTGGCTAAGATATCTATATTTTGATAAACTTTTTGGCTTTTGTTAAACTGCAAAATAATAGTTTTGTTTAATAACATATGGTTATTTTGGTTTAAATATCCAGAGTGAAATCCAACCATTCTAACCATAAATTTTCCCTTGTTTAAATTAATATAAGCATCATCATTTTCTAGCTGAATTACATCATTTTTTGAAGGCAAAAATTCAGTATTTTTCACTAAAAAAGTAATGTATTTTTTCTTTAAATAGTAGTAGTAAATATCATAAGGTATTTTGGCTTTTAAGGCTTCGTTTTGATTTGGATTTCTGCGCCTTAAATTTTGGGTTTTTATTGCCGTTAAAACATCACTTAATCAATATCTTTTTTTACCCATTATTTTAAGAATTTCGTTATGATAAAAAATGAATTTTTTGTTAGTTTTTTTGTCTAAATATCTAGTAATATTAATTGTTAATTTTTCACCATTTATTATTAATGTTTTTTGCTTCCTTTCAAATACAATTCAATGACTATATTGATTATTTTCAAACCTTGTTTTAGTACGAAATATTTCTTCTTCGATGTTTAATTTTTCTACTATTAATTGGCCTGAGGATAGGATACTTTTTTCACTTACACCATTAATATAAAAATCTATTTCCATATAAAAATTAAAACAAAAAAGCAAGAATATTCTTGCCTAATGTTTTAGATTATGGGATTTTGAGCGGAATTGGCTAGCTTTGGATTCTTTCTGAGATTTGTTATATTCGTTGATTACTTTGGAATCTTTGAGATCGGTTGGGGTCTTATCAAAATCGGATAAAGATGAGGGAATTTGATGAAATGTATATGTATTTGTTTGAACATCTAAAATATATTCAAAATTAGTATAATTAATTTTTTTAGCCTTATTTATGCATAACTTTATGTTTTTTAGGGCTTGCTTAGTATATTCTATATTTGACGTGTTTGTATCTTTAAGAAATTGGGATTTATTAAAATTATTAACATAATTATTTTTTATAGTTTTGTCGATATTAGAATAAGATATTGAATTTAATTGGTTATTAATTAACCCTTTAGTTGTATATAGGACTAAATTATTTTTTAAGTTATTTGATATTTTTAAAATATTTAGATCGGGAGGATTTGAATTAATCCTTCGCTCTAAGTCATTAATTATTAAGTATTTATCGCTTTTATTTAATGTTGAGGTTTCTTGATAAATATTTTGATCTACATTAGCTTCTAAAATCTCTTTGTCAATAATAAAAATATCTTTTGTTATGGCTACTTCATTAAATTTATTTCAATAATTTAACTTTTGATATGTACCATTATCAATTTTTATATTGTTCTTAATCGTGGAAGAATTTGCGGTTTGTAGTCTTATGCAATAGTAAGTTTTATTTTGAATATTTTCAAAAATTATCAATGGGTGGGCTTGGACTTTTCCATTATTTAAAACTTTGCCATCAATTGGCAACTTAAAAACATCTTCTAATAAAGGGTTAGTAATTTTTAACGGTTTTCATAGTTCTAACATATATAAAATTATACAAAAAAAGAGGAACAAGCCTCTTTTTAAGACGAACCAGTTTAGGGGGAATGTAGCTACACGAACTAGCATGCTACTGTTCGATTACATACGTGTATCGCCCTAAATTCAATTAGATTATATCATAAAACGATTTAATTTTATAATATTTTTATAAACTTTTTGGAAATTCACAAAGTAATCTCAATTATATATTTTGTTTTTTGTTTTTTATCCTTTTTAGCTTTCTTTAAGCTTGATTTGCTTGCCTAATGTTTTAGATTATGAGGTTTTGAGCGGAATTGGCTAGCTTTGGATTCTTTCTGAGATTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTTTTATCAAAATCGGATAAAGATGAGGGAATTTGATGAAATGTATATGTATTTGTTTGAACATCTAAAATATATTCAAAATTAGTATAATCATTTTTTTAGCCTTATTTATGCATAACCTTATGTTTTTTAGGGCTTGCTTAGTATATTCTATATTTGACGTGTTTGTATCTTTAAGAAATTGGGATTTGCTAAAATTATATTTGTAAAAATTCTTTTGGTTAATCAAAACTGATGTATTTTTATATGCAAGAGTAGAAGACAATTGAGTAGAAATTAAACTATTAGTTGTATATAAAACTAAATAATTTTTTAAGTGGGTTGAAATCTTTGTTATATTTAAATCGGGTGGATTTGAATTAATCCTTCGTTCTAAATCATCAATTATTAAAGATTTATCGCTTTCATTTAATGGCAAGGTTTCTTGATATATATTTTGATCTATATTAGCTTCTAAAACTTCTTTATCAATAATAAAAATATCTTTAGTTATTGCTACACCTTTATGATTTTTTCAATATTCGTTGGTTTGATAAGAGCTGTTATCAATTTGTAAGTTATATTTAGTTGTGTTTTTTCTCGCGGTTTGTAGTCTTATGCAATAGTAAGTTTTATTTTGAATATTTTCAAAAATTATCAATGGGTGAATTTTGAATTTTGTGTCGATATTTAGATGAAAAATATCTTCTAATAAAGGGTTAGTGATTTTTAATGGTTTTCATAATTCTAAACTCATATAAAAATTATACAAAAAAAGGGGAGCAAACCTCTTTTTAAGACGAACTAAGACTTAAGACCTATCATTAGGCTTTACAAAAAATTAAAGTTTATAGGCTGCCTACTATTCGATTACCTATAAATATCCTTTCTTAAGTTATAATAATTATACTACAAATAAATTTTTAATTTTATAATATTTTCAAAAAATATAAGGGGGTGAGCTTAGATTTTTCCGTTATTTAAAACTTTGCCATCGATCGGCAACTTAAAAATATTTTCTAGTAAAGGATTTTTTATCTTTAATGGTTTTCATAGTTCTAAACTCATATAAAAATTATACAAAAAAAGAGGAATAAACCTCTTTTTAAGACGAACTAGAGCTTAGAGGGGTATCAATAGACATTTAACACATAAGCCTTATAAGCTTGCCTATTTTTCGATTACTTATAAGTATTGCCTCAAGCTAATTAAAATAATTATATCATAAAATGATTTATTTTTATAATATTTTCAAAAATTATCAAAGGATGAATTTTAAATCTAGTGTCGATATTTAGACAAAAAATATCTTCTAATAAAGAGTTAGCAATTTTTAATAGTTTTCATAATTCTAAACTCATATAAAAATTATACAAAAAAAGAGGAATAAACCTCTTTTTAAGACGACCCAGCCTAGAGTATTTGTAGCTTCACGTCAAGCATGCTACTGTTCGATTACTTACGTGTATCTCTCTAGGTTCATATGAATACATTATATCATAAAACGATTTAATTTTATAATATTTTCAAAAATAATTAATGAAAGTGTCTTTTTTTAAACAAATTGATAATCTTTTACTAAAAAAATTATAAGTTAATTTAGTAAGAGATTTTTTATATTTTTATTTCAATAATTCATATTTTTCTAATTTGGGATAAATGTATTTATCATAAAAATCATTTAATTTAGTCTTAAGAGTCTTAATAGATGAATTTATTTCAGGAAATTCATAATCTTCTTGTTGCTTTCCAAAACGAGTTGTTCAAACTTTTTTGGTATTAAAATCATTCATTATTTGTTTTAAACCATAATTAAATTTGGGTAAAATTTTTGATTTGCAATTTTCAACAATTTTCTTATTTAATTGATTTCAAATTTTTATTAATTCTTTATGATCGTTGTCAAGCAAATTCTTGTCTAATTTTTCTATTGCTTTGCCAGCATAACAAATTTCATTTAAAATTAATTCATTACTATCACTCCAATAAGAAAAACATTCGGTTTTGTCAGTTAATAGTGTTCATAATAAACAATCATTCAAAAATTCTATATCTTTTCAAGCGCTATCTTGTTTATCGGCCGATTTCATTATAGTGAGAGCATCTCTTCTATAATCTTTTCCCTTTCAAGCATTCAAAGCATTAAGAACTGAAATTTGCAAAATATTTTCATTTGAAACATAAAATTTTTTGTTTGAAATGCCTTTATTAAGGTTGGTCAAAATATTTCCATTATGTACAAACATATTGCCTATGGAGAGAATAGCATAAGAAGCATCTTTTTTAACTATTGGAATATTAAATTCATTAACTCTCTTATTAATTTGTTTTATTTCTATTTTTTCATTATCTAATTCATTTGATAATGAAATAGAACTGCATGCATAGGAATTAGGGGAATTTTTCTCTCATCTAATTATAGGAATCCCCCCTTCTGTAGCGTTGTATTTTTTTCTATTTGATAAAAAACCTTCAACAAAATTAAATGATTTTATATCTCTTCATTTTCAATATTTAATAGGAGAAATTAAGCAATATTCATCGCCTACATTTAAAAATTGATTAGCGCTTCATATAAATTGAAAACTCAAATCTCTAATAGTTCCATCTGCAATTTTTGTTTCTTCTTTTAATTTATTATAAATATAACTTTTTGTGATTGAGTGTCCATTGCCATGAGAATTTGCAGTTTCGTCCCTGAACGGAGGGTTTTCAAAGAAAACTATTTTCCCGTTAGGAAATTGATTTCTTCAATTATCTATGTATTTTTTTAGTCATGAATTAAACTCTTCAGTTAATGCATTACCATTTTCCATAAGATTTTCATCAATATAAACATCAATTAATTGTGCATTTGTATATGGAGGCTTAAGCATTCTTACTTTATTTTGAAACTTGCTTCCTAAATTTACTCATTCACAAAATTCATATGTATTCAAAACAAAGCATTTTAGAATATCTTTTGGTAGATTATATTCAAGTTGTCCTGTTCCTGCGCATCTATCTATTACTAAAATTTCCTTGTCAGGATTTTCATTGAATATATTTTTTAAATATGTTTGCATAATTTTTGCATATTCTTTGGGGGTAAAAAATGCGCCTCTGTCTTTTCTATAATCCTTACCTCCAACATGATCTCATTTTTCTGCAATTATTTCTTTTAAATCATTAATAAAAATAGGTTCTACCCAATATAAAATCTTTTTTTCTTGATTTAATTGCATTAGCGCAGATTTTAATTTATTTATTTTATCTAAATTGTTTTGTACATTTAAACCAGAATTTTCTACACACTTTTCTATATCTTTCATTCCTTTAAATAAATCTTTTATAAAAATATCAGGGGTTTGAATAAATTTAGAATCATTTTCTAGTTCTAGTTTTTCATAAAACTTGTCATTTATGTTGTGAAAATTATTAATATTAAGTTTTGTTAATAAATATTTTTCATCATTTTTGGTCGGAGTTATTTTTAAAACAAGATCTTTAGGATTCAATCATTCTAAAGATTGTTCGTTCTCTTTTAAAACAACTGGTTTGGCGCTCGAAGGAGCCGTATTTAAAGTATTGTTTTCATAAATAAAATTTTCATAGTTTTTAGCATCAATAAGTCAATATTTATGGTCAGTAAAAGTTGCTATACATATTCACCGTGGAATTCGTATTAGACCATTATTATTAAAATTTTTTAAATAAGATAAGGCTTCGCCTAATGCTTTTTCCAATGTTTGAAATTGATCAAACTTTTTTTTGAATTCATATAAAACGCCTTCATTTATTCCATCAGTACCATTTATGATGTATTTTTCATAATATGCTTGATCTAAAATTTTGTGTGTGGAATCTTCATAAACGTTTTTTTCATAAAAACTTCAAAACGCCCTTTGACATTCTAATTCACTATATTCTTGTCAATTAAATTCTTGATTTTGAGAGCTCTCTCTCTCTCTCTCCATTATTTACTATTCAGGTTCTTTTATTAAACATTTTTGCTCCTTAAATTAAATAAATATAACTTTTAAAGTTTCATTTGACAATAATTATATATTTTTAAAGGATTTAATTAATTAAAAACACCAAGTGCAAGAACACTTGATGTTATTTGCTAATTTATAGAATTTTGCTCATTATTGGTTTTTGGTTCGTTTTTTGTTTTTTTATCATTTTTAGCTTTATTTAAGTTTGCTTTTTCTTGCTTTTTTGTTTGGCGTTTTAAATTTTTTGCTTCCTTTTTGGCTAGTTTTTCTTGACGTTTATTTGAGCGTTTTTCTTGATAAGATTGCTTGTTTGGATTTTGACGTTTAATACGTTTAGCTCTTTTTCCAAATGATCAACCGTATTTCCTCAATAAAGATTTAATTATGAAAATAGAAGCAACTAAAAATGTTAATATACCTACAATTACCACTAAAGGAAATCATCAAGTTTTCACGACTTTCTTTATTCTATTTGGGTCTTTAGGTATATCTTCGGGACTGACAACATCCTCATCATCGGGATTTGGATTTGGTTTTCAATCCGGGTCAATTGCAGGATTATATTCTTTATTAGCCTTATTGATAATATATCCATATCCAAACCCGATCGTTTTGTTTGTAGGTAATAAAAGAATTTTCTTGTATAGAACTTCTAAATTTAGAGAATTATTATATATTTCTCTTTCGAGTTCTTCAATAATAGAGTTTTTGTATTGTTCGAGTTGGTTTTTAAGATCTAAATCCTTAGGCTCGAATTCAATTTCTTTATCCTTACCTTCATATTTAAATGTTTTGCGTAATTGTAGAGCCTTTAAGGCTTGTTGTAATTTTTCATAAAATTCCTTGTTTTGTTTTAGTTTAGGATCATCATATTTTGAAACTAATTCCAATCAATAAGCATCGTTATTAATTGCAAAGATTTGATAATCATTGTTAATTTCTAGACCTAATTTAGACGCAGCATTAACTACTATTTCAATAACTTTTTTTCTAATTTGACTTGGCTTGGTTTCTTGAATAACATTCTTGTTATCCATTAAGATGTAACTTAAATTTCTTAAATTAAACTTACCTCCTTCGCCTAAATTTTTAAAGTTAATTATTTTGTAGTTATGCAAATTAGCGTTTTTAGAAACTAGTTTTAAACCAACAAAACTACTTAAAGCCTTTCCTCTCATCAAATGAGCAATTACTTCATTTAAATTAGCAATTTCAACGTCTTTATTTAGTAACATTTCAATTTCTTTGCCAGAAGATTTTGTTTTTAGCTGATATTGCAATAATTGATTTGTAATGAAATTTATTACTTCTTTTTCAATTGCTTTTCTATATCTTTCATCAACAACATCTTCGGGGATGTTTTTTTCTTCGAAAGTTTTTTTAGAAATATTTAAAATAAATTCATTTTGTATATCCAAGGTGCTTAAATCAATAGGTGGATTATATACGTGTCATGCTTCATTTCTAAAATAAATTTCTTTTCTAGAATTAGCATACAAACCTTTACCAATTAATGTTAGTTTAACACCTTCATAATTTTGGTTTTGTTCTGTGCCATAAATCGAGACATTAGCAAGCTCTTCTAGGAATTTATTTTCCTCGGTAATGTTGCTAAATTCAGAAATAAACCAATCTTTATTTTCAATTAAATTCTTGGTTAATGTGTTGCTTGACATATTTGTTCTAATATATTCAAGAACTTTGTCTTTTATGGCTTGTTTATCAGTTAGACCATTAAAATTTTGTAATTTACTTCATTTAAAGTCGTTAAAAATATTAACTGCCTTATCCTCATCATCGGGATTTGGATTTGGTTTTCAATCTGGGTCAATTGCAGGATTATATTCTTTATTGGCTTTATTGATAATATATCCATATCCAAACCCGGTGGTTTTGTTTGTAGGTAATAAAAGAATTTTCTTGTATAGAACTTCTAAATTTAGAGAATTATTATATATTTCTCTTTCGAGTTCTTCAATAATAGAGTTTTTGTATTGTTCGAGTTGGTTTTTAAGATCTAAATCCTTAGGCTCGAATTCAATTTCTTTATCCTTACCTTCATATTTAAATGTTTTGCGTAATTGTAGAGTCTTTAAGGCTTGTTGTAATTTTTCATAAAATTCCTTGTTTTGTTTTAGTTTAGGATCATCATATTTCGAAACTAATTCCAATCAATAAGCATCGTTATTAATTGCAAAGATTTGATAATCATTGTTAATTTCTAGACCTAATTTAGATGCAGCATTAACTACTATTTCAATAACTTTTTTTCTAATTTGACTTGGCTTGGTTTCTTGAATAACATTCTTGTTATCCATTAAGATGTAACTTAAATTTCTTAAATTAAACTTACCTCCTTCGCCTAAATTTTTAAAGTTAATTATTTTGTAGTTATGCAAATTAGCGTTTTTAGAAACTAGTTTTAAACCAACAAAACTACTTAAAGCCTTCCCTCTCATTAAATGAGCAATTACTTCATTTAAATTAGCAATTTCAACGTCTTTATTTAATAACATTTCAATTTCTTTGCCAGAAGATTTTGTTTTTAGTTGATATTGCAATAACTGATTTGTAATGAAGTTTATTACTTCTTTTTCAATTGCTTTTCTATATCTTTCATCAATAACATCTTCAGGGATGTTTTTTTCTTCGAAAGTTTTTTTAGAAATATTTAAAATAAATTCACTTTGTATATCCAACGTGCTTAAATCAATAGGTGGATTATATACGTGTCATGCTTCATTTCTAAAATAAATTTCTTTTCTAGAATTAGCATACAAACCTTTACCAATTAATGTTAGTTTAACACCTTCATAATTTTGGTTTTGTTCTGTGCCATAAATTGAGACATTAGCAAGCTCTTCTAGAAATTTATTTTCCTCGGTAATGTTGCTAAATTCAGAAATAAACCAATCTTTATTTTCAATTAAATTCTTGGTTAATGTGTTGCTTGACATATTTGTTCTAATATATTCAAGAACTTTGTCTTTTATGGCTTGTTTATCAGTTAGACCATTAAAATTTTGTAATTTACTCCATTTAAAGTCGTTAAAAATATTAATGGCATTTTCAATGTTGTTATTATCGTTATCAGCAAATTTTCAAGATGAATTAACGCATTCGTTATAAAGAGGCAATAATTCATCATATGTTAATTTGTCTAATTGTTCTTTATCATAATGTTTAGATTCAATTAACCATTTTTTAAAATATATTCCAAGTGTTGATTGTCAAAATGGCTCAAATTTTTTCATTAAATCAATGCCATAATTATCTTTTTTATTTTCTAATTCTTGCCAGAAAAGATTTTTTGGTGAATTTGATTCATCGATAAGCACTAATTTAATATTTGAAATTGATGTAGAAGTAGCAGCATAAACTATTCAGATGCCTTCTTCAGACATATAAGCATTTTCACTATTACCGTTTGATGAAGATAATGGTTCAGGCTTAATTTCACTAGGACTAAGCCATTTGTTGCTTCCTTTATCATAAAGTTTGCATTTATAGTATGTAACATTAGATTTTTTGGCAATATTTTTATCTAAAATTAGATTTCTTAAGGCTCCTTTACCAAGGACAGCACCCTCTGCAAAAATACCAAAATCATTCAACATTTTAATATCGGGATATCAAAATTTTAAATTTTTTGTTATTTCTTTAGCTAAAGTTCCGTTTTTTATTCATATGAGTTGATTTTTAGTACCGGTTTTAGGGTCTATTGTAGGATCATATTTAGGGTTAGGTAATGGATTACCTTCTTTATCCACTAAAATTCTTCCATCTTTTGTTATTTCTTTAGTAATTAGTTCTTTTTGTTTTGGGTTATTTTCGGGGTCTCAAGCAAACCATTTAAATGATTGGTTGATTGGTTGTTCAACTATAACAAATTTAATTTTATAAGTTGCTATTGGCTCTCCGCTGTTATTTGTGCCAGGTTTGAATTTCTTTATTTCAACTATATACTCGTTTTTCTTTCTTGAATTATGGTCATTTAACTCTTCTTGAGGCTTATTTGAATCATTTTTCTCTAATGCTATTTTTTCTTCATCAGATGCCGAACGTCTTAAATCTTTTAAATGATAAACAAAATGTCTATTTAAAACATCAACTTTTTGACCATTTATAAATAAAACTTCATTTTCTTCTAATGTGGTGTCAAAATTTACTTGTAGTGGAGTATGTACTAACCATTTTCCCCCGTAATTACCATTTGCAGGCTCTAATTGTATAGGTTTGTCTTCTTGCAGGTTGTCCTTGAAATTGAAACTATGATTTTCTTGAATATTAGATAGGTTATTTTGATTAAGAACTACACCATATTTAAATCATAATCTACTAACAAATCCTGTTTTGTCTGCATAAGTTTGATAAATTTGGCTTGGAGTAAATCTTACTGGAACATTAGCCATTATTGTCTTGCCTTTAAAAAGACTATAAAACATATGATAAAGATTCTTAATTTGAATTTTACTTTGTTGATTTTTAGCAAAATTATTAGCAAAGTTATAAGTATCTGCTTCATTAAAAATTTTAGAAGCAAAAGAATTAGTGATATCTTTTCCCTTATATTTTAATTGTTTGATTTTAAAAATTAGATTAAAATGATTTCCATCTGAAATAGGTTTGACTTCATAACCATATTTAATAATAAAATTGCCAATTGATGTTTCATTATCCATTTGTTCAGCAATTTCTTGTAGTTTTTCAAGATTACTTTTACCACCTTTAAAAATATTTAAATCAATACCTGTCGTTAATTTTCCCATAGTATCAGAATCTATAATAATTTCTTGACTTGAATTTTGTAGATGAAATGCTGACATGAATTTTTGAGAATGCTTAAAATTATTCGAATTTGCTAAAAAACAAATTAAAGAATAAGCATCCTCAAGATCTATGCCATGTCATTTTGTTAAATCATAATTAATTTCTAAATGATCATTATATGACAATTTAAATTTATTATAAGCAATATATGCATTATCAAAATACGCAATATCAGACGAACTAATTTTTGGATCACTAAGTCTATTATTAAATGTTTTAATTTTTATATCATAATCTTTGGTGAATTTTTTAAAAGATTGGGCATTAGCTCAGTTAATACTATAATTAGATTTTTTAATAGTTTGAGTAATTTTACCTGGCTCTCAAATGGGGTCAAAGTTATAGCCAGGATTATCGGGATCTTCGTCTTCTCTTCATTCTCCTCCCGTTAAACTAGAAACGTATTTTTTATAGCCTTGCAATCAATAAGGATTTTGAATGTATTTGATTTGATCTCTTAGGCCTTTTGTGGCATTATCTATTTTTTCATCCAATTCTGGAGTTAAATATTTGTGCTCCTTTGCTCATTCAGCTTGCATGTCTTGCTTTATTTTCATTAACTGACTTGCCTCATTTTTTCCGGCATCATTTAGTAAAGTAATTTTATAATCGCCAAAAGGTTGATTATTTATTTCATTAATATTATTAATAGATAATTCTTTTAAAAATTTTAAGTTATCATCACTATTTGCAATAAATCTTTGATCAGAATAAAGAGGGTTATCTGAATAACTTTTGGAATCAAAAATAAAACTATCTTGCCTGCTATTTTTTCAATATGTTAAATTTGTAAAAAAACTTTTTAATAAATTTTTGTTGTTTAAAAAAGAATATTGGTCTTTGTCAATTTCTTCATATTTATAGCTTGAATAATGTTCATTTCTAGAAAAACTCAAAGAAAATAGAGAAGCCGAAGCAATACTAAATATTCCTCCGGCTATCAATAAGGTTTTTTTAATTTTCTTCATCTTCAAAAAAATCTATTCCAAAAATATCTTTTTCGTTATTTTCTAAGTTGGTCTCTTTTGAAATGGGTTGAATATTTGATTTTGACTTTGTTAAAAAAGTAATATTTTTAGCAATTATTTCTGTTTTATAAATAGTTTCGTTATTCTTTGAACTAAATTTAGCATTGTGTAATTTGCCAACAACTTCTAATAAATCTCCCTTTTTAACATATTTCATAACATGTTCTGCCAATTTGTTAAAACAAGTAATCTCGATAAAATTGGTTTTATTTGCTTGTTCAAATAGTGCAATAACAAAGGGCAATGCTTGTCCTTTTTCGCTTTTAAAAGGTTTTCAATTATCATTTACTAGCCTACCAGTTAATATAACTTTATTCATTTATCCTCCATTGCACGCGTGCGTGTATTATGCACGCACATGTGCTATATATTTTTATTTTTCTTCTATTTGTTCTTGGGCAGTAGAATTAGTTGCCCCTATTTCTTGTAAAATGTCATCAAATTCATTTTCACTCAAACTTGAAAGAAAATCATTTCAAGCACGTTCTGAATTTTGAATTTTATTTGCCATTTTTTTAGTTCAATCATTAGTTAATTTATTAACAGCACCTCTTATAGTGCGTTCAAAGAACTTAGATTCTGCTAATATATCAGCCCCGGGATTATCAATTTGTTTTTCTAAATCTCCATTATCTTTAAATAAAGCATTAATAATCATATTAAGTTTTAAATTAATAATAGACATTTCTATATCCATGCTTCTAAATCTTCGATTTATTTGCAATTGATAAGGACTTAATGCACTTCAACTAACTTTTCTTAGCGCTTGAAACAAATCATGTTTTCAATCTCGCATAATTTCTTTCTTTTCATTTGCTAATAAATAACTTCTAATAACATTATTTAATTCTTTATAAACAGTTTTTCCATTTTTTTCTAATTCTTTTTTTCAACGATAAAACATTCCTACATCTTCAGGATCAGTAAATCGTCCTGAAAATTGTGCTTCAAAACTGTTTTGTTGCTTTCTTTCCTTTTGTTTTTTAGGTTGGGAACTAGTGATAGAATTCATAATGTTATTTATTTGATTATCACTTGTTAAATTAATATTTTCTATAAAGTCATCTTCATTTTGAGTTTGATTGTCATTTGATAAAATTTCATTATTATCTAAATTATCAAAATCTTGATCTAATTCGTTTTTATTAGTTTTCAACTAAACCTCCTTATGCACGTGTGCGTGTATTATGCGCGCACATGTGCTATATTAAATTCATTCTTTATTTAAGAACTTGAAGAATAATTCTTGTTCTAATTTGTTGTAATAAATTTCAACTCTAATTCTTTTATTTGAACTTATTGAAAATAAACATTTTCCATATCTTGCATTTACTAAATATCTAATTTCACTTTGTAATAATTGATTAGAGTTTTTAAATAATTGTTGCACTTTGTCAATATCTTGGTCTTTTAGGCCAAAGAATACAGAATATTGACAGTTTTGAATAATTCTAGATGCATCATCACTTGTGGCATTATTAATAGTAAAATCACCAGGATTTTGGGTAGTAAATATCATACCTGCATTGAATTTTCTAACCGTTTTTGTCATTTGAACCATATATTTTAAAATAGCAGGATTATCAGGATCTATATATAAATGGGCTTCATCTACGATAACGGCGCAAAATCTAATAGTATCTTCTACTTGCTTTGCATCCATTAACTTAATGTTATGTTCTTGCAAATATTTTTTCTTTAACATTTCATTATGCGTTCAATTATTAAATACTATTTCACTAACAAAGTTTAATAAACACATTGTTCCTAATTTAGCGCCGATATTGTCCGCTCCATTTATTAAGTTTTGCGTATTAAATACTACAAAATCATTATCAAGTTTTATATTAGTGTGTCCATTAAATAAGTTTTGATATCTTCCATTATTTTCAAAAAGAAAGCTAAACGATTCGCAAAGTTCAACTAAGATTGCTCTTTTTGCTTCAGGATTGTTTAAATGAAGCGTTTTATAGTTGGACATATATTTTATCAACTCACTTATTGTTGGCCATTCTACGGCCTTTAATTGCTCAATAGAAGCAAGTTTATATATTCCTCTATCTTCATAAAATTCTTTTATTACATTTTGAAGCAATATTCACTTATGATCTTCTATATTAATTAAAACCTTAAAAAAACTTTCTAATCACGACAAATGGTTGTTAATTAGTGAAGATGTATCATTACCCGTTGCATCCTTTAATGAATTACGGATTTGAAGAGGATTGATTATGGTGTTTTTACCTGCACCCAAATCAATAATTTGTCCATTAAGTTTTTGAGCTAATTCTCTATATTCTCCTTGCGGGTCAATAACAACAACTTTATTATTTGCTGCTAAAATACTAGTTAATAACTTCATGGTAAAAGTTGTTTTACCTTTTCCACTAGTACCATTTATAAACATATTAAAATTAGTTCTATTTGAATGATACATATCAAATAATCTAAAACTTAGAGGACTTCTTGTGGCTTCTGAGTAGCCTAGAAGTAAATTATTCGAATCATTGTGATTTTCGGTTTCAAAAGGCCATCCCAAAGCTAAATTAATAGATGTAATTTGATAATATTCTCTTTCTAATCTTTCAAAAGGATAATTGCAAGCCTCAGATAAACCTAAAAGTTGTCTATATTTTAAGTCATTTAAGCTTATATTCTTTTTACTTGCTATATTTCTAGCAATTCTATGCTGTCTATTTAATTCAGCAAGGTCTGTGGCTTTTGATACAAAGAAGAAATTACTATCAAATAGTTTGTATTCATCTTTATTTACTTGATACATCATTGCCATTGTCGCTTGTTCATCAATAGAACCGACCAAGTTTGCCGATACTGATCCTGTTGCATTAGTAGTATCAATAGATGCCATCATGGATTTGTCCATTTTTTTATTGGCAAAATTGTTATTTCAAGGGAAATTATTTCATATACAAGTTCCGTTAATAGAAAATAAGGTTTTGATTCATTTATCAGACAATTCAAATGGTAATTTGTTTAAACAATTGATTTTTACATAATTATCATTGATTTGTAAATATTTGTCTCTAATAATAACTTGATTATAAATAAATAAGTTATCTAATGAATTTTCAACACCAAATTTAGCATCTAAATCATTGCAATATTCTTGTATGTTTTGGCTATCAATACCAAACATATTAAGTTTATTAAGAAATGTTAGAAGTCTTGCTTTATGCAATTTTTCTAAACCTATTCCAGTATCTTGAAATTTATCAATTATAACTTCGAGATATTCATTTAAGTCATTAAGATTTTCACCATTTACTATTAAATAAAAGTTATTACTTATTGCTTCTTTATTTAAATTAGAGAAATCTTTTATATTAAGGTCATAATAATTGTTTCACATTTCATTATTATTTATTTGCTTTGCTTGTTTAGCTTCAATATATTTAATATTTTCTTCATAATTTACTTTTTCTTCAAGTTTAACAAAAGAAACTTTGTAAGTAAGTATATTTAAAGTTTTTGCAAAACTATCAATAAATGCTTCACGAGTTAGTTCATCCTCGTTTCAAATATTAAGACCATTTAGTTTAAATACCGCTAAATAGTTTTCTTTATCATTTCTTGGATTATAAAAACTTATTTTTGAATTTTTATTTTTGACTACATATTCATTAACTAAACATGAATAAGGATTTAAATCCAAACTACTTCTAAATGAATTATTTTGAATGTTTTTTACAAACCGTTTTGGTATAGTTCAAAAAACAAACATTCTTCATATTAAAACATATACCTTGCAATTATTCTTTCGATTTGGCATTATTAAAATAATTCCTAATAAAAAAGTAGGTATTCAAAATGCAAAGAAAATTAATTTCACATTTATTAATTGGCTAAAACCAAAACTAATAAAAAATGATAATAATAGAATTACAACAAATTCAATAAGATCCTTTGTTTTGAAAGTTTTTCAAATTTTACTACTTCTATGTTTTATTCTACTTGGCTGTAATTGCGTCATATTACTCCTTATACTTTGCTATTTTAGAAAACTTGCAACTTTATATGCTGCACCTACATTCGTGCCCCTTAATGCTGCATCCTTTGCTGTTTTATATGCTCCTGCCCCTGATTTTGCTCCAGACATAGCAAGTCTTGCAGATTGGCCACCAGAAACTTCACTTCCAACAAAAGCAATTGCCATTTCTGATAATTTTTTGAAAGCAAATGCAGAGCCAATTACCAAGAAACAAGTTGATAATAATGCTAAAACGGCTTTGATAGTAGTAAAGTTTGATAATGAAGAAAAGGCTCCTATCATATGATTTGTAGCACTAGTCATAAATGTATTAGCTAAGCCTATAAAACTACCCAACATCATAATTCCTACACCATAAATCGAGCAGGATAATAAATTACCAAAATATTTTTCCTTTCATACTCTTATTCTCTTTCCTCCATCATTTATAGAAAATGCAGGAATTAGTGGGAATGAAATAAATAGTAATCAATTAATAAATACTCTTGAAATAATGTCAAACAATACAAATATTAAAGGAATTGATATTAATACACCTACAATAGCTATTGATAAGCCAATAGTAAGCGCATAACCTGTAATGTTTTTTGTTCAATCTCATCAGGCAGGAAAGCCCAAACCTCACAAAGGATTTGCTATAGGAGATCCGTAAGGGAATGGATCTCAATATTTATCAGGTACAAAGGCTTGTGGAGGCATCATATTTCTAATAATATTATTTGAAATTTGACCGAAACCAAAATTAAAGAAATCGTTATAATCTCCATATAAGGCTTTTGTTACTTCAATAAAGATAATATTAATAAAGAGAATTACTACTTGGAATAATATTAGCGTGAGGACGCCTCCTACCGTTGATTTTAAGGCCTGAACAAATATTTCCTTAGCCTTTAATCCATTAGATTCTTTTGAATACCTAATAACAAACACAAAAAAGTTTATTAATCATAATATAAAACTAATAATTAAAAATTGATATCAAGGTCTCGCTACAGAAAATTGGCTATAACTTTGACCTGGCAATAAATTAAACATTAAATAGTTGTTTAAATCGAGGCTAAACAATTTAAACAAGAAAAACAAAAGGTTAAATATAGTAAATCCTATTAATCCAATAGCAGCAAGAGGTACAAACCACATAAGCCTAATAGGCGTTGCGATTAAATTTATTATAGGATTTATTATCGCCTCTAAAAATTTACCCATATTACACCATTAAATTAAAATTAGTCAATTTAGGTATTGAAATTGTAAAATTATTTAGTATTGCATGTGTAAATAATGATGCTTGTGAGCCAACATTAGATGGTTTTTTAACATTAGCCTTAATTAATTCAATTAATGGTCACATTACTACTAAAATAATTAGTGCAGATAAAGCACCTCATACCTTTAAATTATTTTGCTTTCTTTCATTAGGGTCTTTTGTGGCAGCATTTTGACTTCTTGCTCACAATATCATTCCTATTAATAAAATAATTGCAACTGGAATAAGGGCTATAAAAATAGACCTCATTATAGTTTCACTAAAATTTCCTAAAAAGCTAAAGCTTTTATCATCGATTTTCATTTTTTTCCTTTCATACTCATTAAAGAGTTATTTTAAAAATTAAAAAAATAAATAGAAAACTAACAATTTGAAAATCAAATTGCTTTTTTCTATCATTCAAAATTAAAAATAAGTTGTGGTGTTTTAAACACCTAATATAAGTTAAAATTCTTTTAAATCAACATTTATATTAAATGCTGAATTTCATTTTTTTAAAACATTGTCTAAAAACAATTTTTGTCAATGTTCTTTATTATTTACATCTAAAAATCCATTTGGTTTTTTGATTTCATCAATGTCTTTGTATTTAGCCTTAAAAATGACATTTAAATATTTAAAGATATAGTCATAAATATATGAATCTTTTCTATTTCTATTTAACATACCACTTTCTAGATTATCAAATTCAATTGGCATGTTTCAACCATTTGCTACCATTGTAGGCACTAATTTTTCATATAAAAATTTATATGCAAATCTATAAGTGTAATCAAAGTCAATTTTGTCGCCCATTGTTAAACTCTTCATTGCAAACAAATCTGCTAAAGTAGAATGTCTATCATTAGGATCTTCAAATTCTCCTAGTAGTAAATGATGTCAGTCAATAAATTTCACTAATGCTTTTGCTAATTCAGTTGAAGCATCTCTTAATTTATCTTTTTTATCACTTTCGATTTCACTTCATTTTGATTTATTTTCAGAGTTTATATTATTAAATGATTTTAGTCATTGAATTAAATTCTTATAAACTTTGAAATTTAATTTTTCTGATAAGTCAAATCTTTCCATTACTAAGAAATTAAATGCTACAGGATTGTAGTTATTATAAGCAGGAGTAAGAGTTGTTTTATCTAGACCATTTTTATCAGTATACATATCATAAGTTTCTTGTGGGAATTTATTTCTCATTGTAGGGTTAGATCCAAATGAAAAAGGAGCAACAAACCATCTTTTTGCTTTATTTTCAAAGATTCATCCTTTATACATAGCCTTTTCATAATCAGATATTAATTCTTTAACAAAAGGATCTTGATTGGTTTTAGCTATGTCATCAAATGATTGTTGAATAAATTTTTCTCTAATAAAAGTAGAAGGCATTTGTGAATCAGCAGAGTAAATAAGTTTTTTTTCAATTCATTTAGGATCTTTAGCGAAATTTTCCTCTACATCATTTATGCTTTCTTTATCAGTTAGACCTTTAAATGCAATATCAGGATCTAAATAATCATCCATTTCATCTAAAAATCTCATTAAGGCTTCATTATAACTTTCATTATCTTTAGTTTTAAAACGACCGCCTTGGTTGATACCTTCTTTGTTTTCATCACTTTCAGCTAATTTTCTAATAATATTTCAAATGCCACAAGCACTTTCACAGTTATTTGTAAAATTTGCGTCATTAAAAACAATACCTCATTTGTTTAATTGGTCTTCTAATGTTTTGTTAGTTAAAGAGAATCACATTTTAGGTCTTCAACGTGTTCATTCATGATTGTTATAATCCTCATCAGATAAACCCACAGGGTCATAAAGTAATTTGCTAAAATAATAAGTGTTATTCTTTTTTAAATAATCTTCATTTGTTGATTTATAAGTAACATAACCTTCTTTTTCTTCTGCTTTAGGTTCCGGGGTAGGAACGGGAGATGGAGGTGTTGGTTGAGGTTGAGGTTTTGGATTTATTAAACCTGGATTTGAACCTGGCGCTGGGGTAGGATTAGGATTATTTTTATTAATCAATTTATCAAGTCATTCGCAACTCATTGCCATAATTGGTAAAGTAGTTAAGGCAATTGTAGAGCCTATTGTTAATAAAATTCTTTTTGATTTCTTCATATACTTCCTTATATTATTTATATATTTTTTAAGTCTTATAATGACAATTTTTATTCTTTATTATTTGTTTATTTTTTGTATTGAAATTTGCTTATTTTTTATTTATTTTAAGTATATTATAATTATAAAACTAAACAAAGGATTTTGCAAATTTTCGTCTTTTTCTACCTCATATAAATGGATAAATTAATGTTGAGGCTAATATTAAACAAATTATAAGTAGTGCAATATTGAATTGTTTGTTTGTCCATGACCCTTTTTCAACAGAAATAGTCATACCCGAAAAATAAAATGCTAAAAATAATCAAACGACAATTAAAAATATAAGATATCAAATAAAACTTTTTCAAGTTAGCCAAAAAGGCAATAGTAATTTTTGTTGTTTAGTAATTTTTAATCCTCCTTATATTTAATATTTATAATTTGTTTTATTTAGCGCCTTCTAAAATAGAAAGAGGTTTATTTGTAATTCCACTACAAATTCATAGAGAATGAAAAGTAGGCTGAGGAATGTTTTTAAGGTCTTGTTTATAATTTTTAAGAAATTTAATTCTTTTTGTTAAGAACACAAGCTCTAAATTTTTTAGTTTTTGCAATTGGCTTACAAAACCGCCAGAATTAAAACATTGAACTCCAAAAATTAAAGCAAACGGTTTATTTAGTTCTTGCAATCTTTTAATAATATTTGCTTTATTTCTAAACGGAGGATTTGATATTATTAAATCATAATTTTCAATAGGCTCATATTTATAAAAATCTTGCCCATTATCAATATGCGAATATATTACTTTATAGCCCAATGATTTTAAAACAATTACAAAATTACTTTGCTGTGTATCAAAGGGGCATCAAATGGTTTTTTTCTTGTCTATTTTAAAATTTTCTAAAAAGTATTTAATATCTTTTTCTTGAGTATATCATTCATCATCTATTGACATTTGAAATAATTGAGAATTATTTATTTATCACCTCCTTTAGCGCTATTAATTTGGTTAGAATTTGCTAATAATTTATTGCGTTTTTTCAAATGTCTAATTATGATAATTAAAAGAATAATTGTTATCATTGTTTCTCCTACACTATGAATTTTGCTTAATAATTTTTATAACTTGTATTCTTTCTATTTGCACCTCCTTAAATTGATTTTTTGAAATAGCATCTTGAGCCATTATTAATGCTTTATTGTATTGTTGCGCAGAGCCAAACGAATATGTTTTGGCATTCTTTCCTGTAGATTGGCTCCCTCTTAATCTATAAAAGTATTTTGACACGTTGTTCCTTATAACTTTCTTGATTTTGATTGGGATTCCGAGGGGGTATGGGATGACTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTCTTATCAAAATCGGATAGGGAAGAGGGATGAGAATTATTTGAAGAATAATGTATAGATTTTTTAAAGTTGTAATAATTTCTACTGGATTTTATTTTTATATTTAATCCTAGTTCTAAACCATATAAAAAGAAGTCTATATTGTCTTTATTAAAACCCGATTGTTTTAAATTCCTGCGATATTTATCAATAAATTCATCTATATGCATCTATTTATATTTTTTGATTCTGAGGTTTTATTTTGATTAGGTTGTGAGTGGGATTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTCTTATCAAAATCGGATAAAGAGGATTTAGAGACTAATTGAGTGGGTTTAGATACGTCAAGGCCTTGTTTTATGCCTTCACGGATTTGTTCCATTTGGCAAAAATCATAGATTGGGTGGCCGTTAGGGTCTAGTTGGGTATATTTAGAGATATCAAGGCCTTGTTTTAGACCCTTACGGATTTCACCCATTTGAAAGCTGTCAAATATAGGGTGGCCTTGAGGGTCTAGTTGGGTATATTTAGAGACAACAAGGCCTTGTTTTAGGCCATAACGAATTTCAGACATTTGGCTACCAACGAAAATTGGATGGCCTTGTGGGTCTAGTTGGGTATATTTAGAGATATCAAGGTCTTGTTCTATGCCAATGCGGATTTGTTCCATTTGTAATCAATCAAAGATAGGTCTATTAAAATTGTTTTGTTTTGCATAAATTGAGACGTCAAGGCCTTTTTTTAGGCCTTTGTGGATTTCCTCCATTTGTCATCAATCATAGATTGGGTGGCCGTTAGGGTCTAGTTTGTTATATTGAGAGACATCAAGGCCGTGTTTTAGACCTTCGCGGATTTCATTCATTTGGAAGCTATCAAATATAGGGTGACCTTTATGATTTAATTGGGTGTATTTAGAGATATCAAGGCCTTCTTCTAGACCAAGGCAGATTTGTTCCATTTGGTCATTATCAAAAATTGTTTTTTTCATATTACCTTCTTATTTTGTTAATGAATGGGATTTTGATTGGGATTCCGAGGGGGTATGGGATGACTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTCTTATCAAAATCGGATAAAGATGATTTAGAAGATAATTGAGTATATTGAGAGATATCAAGGCCTTTTTTTAGACCCTTGCGGATTTGTTCCATTTGTTTTCAGTCAAAGATTGGATGGCCGTGAGGGTCTAGTTGAGTATATTCAGAGATTTCAAGGCCTTGTTTTAGACCGTAAAGAATTTCGCGCATTTGAGATTTATCAAAGATGGGGTGGCCGTGAGGGTCTAGTTGGGTGTATTTAGAGATATCAAGGCCTTCTTCTAGACCTCATCGGATTTCTTGCATCTGCAATCAATCATAGATAGGTCTATTAAAATTGTTTTGTTTTGCATAAATTGAGACGTCAAGGCCTTTTTTTAGACCGTAACTAATTTGCTTCATTTGGTCTATATTATATGAAGTATTTACATATTTTAATAAATTGAATCCTTGGTTTAAACTTGTACGGATTAAGTGCATTTGGTCTGCATCATAAAATTTTTTAACATAGTAAGAATCATCTATTTTTTTGAATTTACAAAAATACTTTTCAAAGTTTAATTTGCTATCACCTTGTTGTAATTCAAATAATTTATTTGCAAAATGGTCTTGAACAATATTTTTGTAATCAAATGATTGTTTAATTCAATATTTTTCAAAATCAATTTTATCTTTAGAAATTTGGCTTTGATTTAGTTCTCAAGATTCCGCTGCTTTTTGCAAGGCCATAATATCAAGTTTCTTTTCAAAATCCTTTGCAATTTCGCTTAAATCATTAACATGTATTTTATTATTTAAATATTCGAAAGTTTTATCCCTAAACAATATATGTTTGTAGTAATCATAATAATTAGAATAAATTGTTTTTATTTGAGCAAGATTAAAATTATTTTGTTTTAGATAATCGTAAATTTGAAATTTTGTATTGTACATGTCTAAGGTATCTAAAATATATTCTTCGGTATAAACCTTTTTTATAGGCACAGAAATTTCTATTTTAGAATTTTGAGAAACTTCTATACTATTTTTATTACTATAGTCTCGGATTTCAAAATCATTATTATTTTTAAAACTAAAAATAGTGTTTTGCTTGTTAATCTTATAATCTACATTAGATCAATTAACACCTGCATCATAAAATATTTTAGAAAGGTTTTGACTATATATTCTAAAATCATCAAATCCTTTAAATTTATATTTATATTTATTATCATTAATTTTTATTAAGAAAATTTTTGGTTTTGCTCACCCTATTGTTTCTAGTGGAAATAAAAATTTTTCTAATTCTTGATTTTCATTTATCATTTTTACCTTCTTATTTTGTTAATGAATGGGATTTTGATTGGGATTCCGAGGGGGTATGGGATGACTTGTTATATTCGTTGATTACTTTGGAATCTTGAAGATCGGTTGGGGTCTTATCAAAATCAGATAAAGATGATTTAGAAGATTTGTTAGTATCTAAGTTAATTAATGATTTTTTATCTCTTGATTCTAAAAGGTCATCTAAATCAAAAGTTTTTAATGCCTCTATGTAATCATCTTTAATTTCTTTAATATTAATTGGCTCTATTTCCAAATAAGGATTTACATAAACATATTCTTCATTTGGCAAATTGTTTAGAACATTAAATTTTGTTGTTCCATTAATTCATTGGCTATCATAACCATTAGCTTTTAAATCTTCATCAATTATTTCTGTTAATTCATCTTTGCGTATAATGTCATTTTCTTCCATGAATTGACTTGCGGGATATTTAAGCCAATCACTTTCACTTATTCAATTATCCGAAGATAAAGTATTTATTATATTAGAACAACCAGCCAAATAATCAGGGCGTGTTTCAGAGGTTTTTAGCTTATCATAAATTCATTGATTTACAAAATCTTCTGATTCTTGCATCTGTTTAGCAATTTTACAAATTTGTTCTTTATATAGTTTTTGTTCTTCTTCTGAATTTAAACTAAATAAAGTATTATTAAAATAATTTAGTACCTTATGATCGTTGTATCAAATGTCATCATATTGAGTCATATATGATATATCATTCATTTTAAATGGTTTTATTTCCATGGTTATTTATCTCCTTTTTCAACTAACTTGTTTTGTTTTAAATAGTACTTTGCATAATATTTTTCTGCATCTTCTCTGCTTATGACGAAGGCTCTGCCATAGGCGTCAATGCCCACGAATTCAATATCATAAAAATCGTTTTGCATTTCATAAATGTTATTAATGTCAAACGCCCATAAATCTGCTTCCATTCCGTATTTTCTTAACTCTTTGTCTGCTAATTCTCAAGCGTCGTTTAATGGATAAACGTCTTGCGGTTCGTGGAATTTCCAATAATTTAAATCTTCTTGCAATTTTTCAGATTTAGCTCTAAATTCTCTTGAACAAAAATCAGATCAAAAATTCTTAACATACATTCCTACTATTTTTTTTCCACAAAAAGCACAATATTTATCTTTTGTCATATTAATCCTCCTTGGTTAATTTAAAAATGTGTTTAATTATTTCTTGCAATACATTAACAACAATAGAATTTCCAGCAAACTTATAAATTTGACTATCACAGAAATTCTTAATTCTTTCATAATCTTGTTGATCATATCCCATTAATAGCATTGCTTCTAATGGTGTAATAACTCTTAAATAATATGGTTTATTATCTATAATCATTTTCGCTAAATTGCTATTATTAGGAACTTTGCTAATTTCTACCTTAACAACCCCTTGATTATTTCAACGCATTTGTTTAGTTGTTATAGTAAAGACTTTGTTCTCAATTATTTTTATTTTATGGTTTGTTATAGCATTAATCATTGAGGGGCAATTTGTTCTAATAAAGTATTTTGGATTTATTACATTTGGCTGTAAAAAATCATTTATATTTTTATATTTATTGTTTAGACAATTAAATGTATCTAAATTATTGAATTGTTTTAAATAGTTTTTATTAATAGAAACAGTAAAAACTCTATTTCTACTTTGGGGAATTCCACAATCTAATGAATTAATTATTTTGTAATGGCTAACATATCCTAGATCATTTAATGCCTTAATGTATGCATCAAATATAGGTTTATGTTTTTTACTTAATACATTTTTGACATTTTCTCAAATAATAATTTTGGGTTTTATTTGAGCTTCTTTAATGATTCTAATTGTTTCCCACAATAAACTACTTCGACTTTTACTTTCTTTACTTCCTCCTATATTTTTACCTGCACACGAAAAATCTTGACAAGGAGATCCGTGAAACAATAAATCAACATTAACATTATTAGGAAGTGAATAATCTCTTATATCTAAAGGTTTGTAAAAATCGTTATATATTGCATTATAAGCATTTACTGTTTTTTTATCATTTTCGACATAATCAATAATTTCAAAATTAGGAGGTTTGATGGTTCTAATTAGAGCTTTTCTAATAGCACCTATTCCACCAAATAATTCAACAACTTTTATTTTATTTATATTCATAGTAAAATTCCTTAATTAGATTTTTCAATTAACTTAGAGACATCTTCAAGAGGATGCAACAAGTAAAGCAATATATCAACATTCAAATCGTGATAATTTGGTATTTTGCGCTCTTTTGCCTCAGATCAAAGAATATTAAGCAAATCGCTTTCGAGATCATTGGTAACATATTGCTCTCACATTAAACTTAATGTTTCTTCTATTTGTTCTTTAATTTGATTTTTTTTATGAGGTATTAATGTACGTTTATAATCATTTTTTAGATTTATATAATTGTTTCAAAGATCTAATTTTCCAAAAACCATCTTCATTCTTTGAATTTTTTGTTCTCGGGATAAATCCTCAGGATCTTCAACATCTTCTAAATAAGTTAAATTGTTATCATCTAAAACTTTTGGGTTTTTGTTATTTTGTTTTTTTTGTTTAGAATTATCAAATTTAATAAGTTTTTCTAAACTTCTAATATCAAATAATTCAAAATCCGCATTAGTTAGTAGCTTACTTTCGTTTCTGGTATAAACATAATTATCATTTGGGAAATATTTATAAGCTAATAAGGTTTTGATTAATAAAGGTTTATAACCATTTTTTATAATTATTAAATCATCAGGTTTTTTAGATTTAAGTTCTGCTACTTCCATAACGGGTTTGGATTTTTCACTTTCAGTAGAACTTGAATGGTTATCACTTGTTGTATAACTTTTTGATTTAACCTCTTTTGTTCCGAGCGCTTTTGAAATATCGTTTAATGTTTGCTCGTCATTAGAGGCTAAAAAATATACTACTTGGCAAGAGTTTTTAATTACTTGGGCTGTTCCTTCTCCATATATGTTATATTTCTTAATTTGATTGTAATCTTGTACCACTAACATAAAGAAAATATTTCTTGAACGGCAAATTGTAAGTTTATTATCAAAATTTACAACAACAGGTAAATTTCCGAATTCATCCAATAGAAAGAAAGCCTTTCTATCTAATTTTAGGTTATCATTTTTGTTTGCTTGTTCAATTAACGATTGATAAATTTGTTCTACTAACATACTTACTAAAAAGTGAGATGTAGGTTTATGGTCAGGATAATGTATAAATATAGCAAAAGGTTTAGGATCAGACACTATATCAACTAAATTGAAACTTTCTGTGTCCCTTAAAACCATCTTTAAATTTTCATCATTATTGAATACATTTAAAACACTTTGTGCTTCGGCTAATATTCCACCAAATGTTTCTTTAGGAGTTTTTGATAAATCTTCTATATTGTTATAAATTTTTTTTCAATAATAATTATTGTCATCAAGTGCTCTCTTTTTAGCATATAAGATTCAAGCAGAGATAGTTTTATCGTTTTCTCAGTGAGAAGTATTTAATAATGAAGCTATTGTTTCAAAATTAAAATTATTTTTATGAATAATATTTTTATCATCAATACCTAATAGCAATAAGAAATTAGTAATAACAATTAATGTTTTTTTAGCTTGGCTAATCCACATATTGCCATTAACTGCCTTACCTCAATTTAAGCTTTCAATAACATTATTTATAACTTCTAATGATTTATTAATTCTTTCTTCAAAAGTCAAATTCTTGAATTTTTCGCCATCAACCGCTAAATCTCATGCATATGAAAGAGGATTTCATTTTAGAGATAATTTAGGGTCGGAGAAATCAATTACATAAATTTTGTAATCATTTTCAGTTAATAAGCCTCCTGTAAATTTGATAATTTCTTTCTTTGGATCACTAATTATTAAATTTGGTTTTTTAGAATAATCAAGTTGTGCCATAAATTCAATATTCGGCAAAATTATTCTATGAGTCTTTCCTGAGCCAGGAGGGCCATTTACAATAGAGTTATCATCTTTATCATTTACATATCAATATTTTTGAGTTTTATCAAAAGTAAATAATCAATTTGGTTCTGTTTCTGCATTAATCATTTTTCTAAATAATTTATTGTATTTTGTTTTAGAGCCTTCTCCTAATTGATAATTTCACAATCAATTTTGGCTAGCCTTTGCACCAAATAAGTATTTTGATATCTTTTTGCCAAGGGATTTTATTTGCCTTCAAAAAATGAAACAGAAGACTAATATAGAAATTCCTAGACCTATATAAACAAAAATATCAGGTTTGGTTTTTAATAAAGTAACAGTAGAGTTTCAAAAAAGTTTAAAATGTCCAGGATTTTTATTCTGGCCCATAAAAACATTAATAATAAAAGTTAGAACAAAAAATAAAAATGTACTAACAATTATTGAAAGAAGTGTCTTTCCTATAATTTTTGATTTTGATAATTTTTCTTGTTCTTCCATAAAACTCCTAATGGACAAGTTTGCTACGATAAAACTCTTCTACTAGTTCAAGTTCTTTTAAATCTTTAGCTAAAAATTCTCTTTCCGCTAGATTTCTAAGGGCTCATTCTGATCTATTTAGGCTAAATATTTTTAGAAATTCATTGAAAAATGAAGCGAAATTCTTTCGGCTAGTAAATGCATTATTATTTTGCTTATAGGCATTTAGTAAGGTTTTGATTATCTTATTACCAACTTGTTTTTGAAAATCATCCATTTCATTATTTATAATTTTTGAAATTCTTTGTTTTAGAAATTTATCTTCGATGTTTAAATTATTTAAATCATTAATTTGGTTTTGATAAGAATTAAAGAGCTTTGAATAATCATTGTCATAATTTAATAAGAATTTTTTTATTGTTTCAATATTTTTTTTATTTTCATCAGAAATGCGAGAATATGAAATATTTTTTTTGTTTAAAACTTCATCTAAAATACTCTTTGAAGATTTAATGAATTCTTGGCACAAATTACTTTTAGAAATAATTTCATTACTATTAGCAAAATTATTAGCGATTATTCCTACAATTTGTTTTCTTGTATTTCAAATGTCTTTTGTAAGCGCATTTATTTTTTGATAATCCTCATCGTATTTAATAGATAAACCCACTTTATAGCCAAAAACATTAATTGAATTTAAATTAAGTTTGGCTTTTTTGTGTTTATGCTCCTTTTCATATCAAAACATATGAACATGAGGATAATCAGTATTTCCATGTATAGAATAAAAAACATTAACATTTTCTAAATCAAAGGAATTAGCTTTTAAAAATCTTTCAAAATTAGGTTTTAAAACTTCATGTCATTTATCCTTACATAAAACATTATTATCTAAGCCTAAATCACCAGGATTAACGATTAATTCTCAATAATTACCTTTTAAATTTTTTAAATCATTCTTAACATTTTCTAAATTTACATCCTTTGGATCATTATCTAATAAGTTATAAAGACCCGATTGTTTAGAATTTTGATTTATTTTTAAATGCTGTCTATATCAATTTATTTTATCTTCCTTAGTAAGAAGTAGTTTCATAGCATCGTAATTATTTTCATCTTCTTTAAATACAACGCATTTATCTGGTCTTGTAATGTAATCTACCACAAGTCCAGAATTAACTCAACTTTTAAAATCGTGAGTATTACCAAGTTTGGTTTTATACATTGTGTTAGGAGTAATAAAATTAAATTCAGTTCATACTTTATCCATTTATTCTCCCTTCTAAAGCATTAGTAATTATTTATATTTATTACTGCTATATATTTATCTTTAAAATTTTGACCGCATATTCGCTCTTATTAATTTGTATTAGTAATTTAACTAATTTATTTATGACTAAAATAGACACCTAGTGCCAGTAACTATCAAAACTTAAACTTAAACGTTTTTAAAAGTAGTAATTTAAAATGTTAAATATTATTTGGTGGCTCGCGTAGGATTTGAACCTACAATGCAGAAACATTAGCCCAATGCTGGGAGCCATTTATTACTGCTTATAAAAACCTTTAAAACTTTTAAATTTGCATTAACAAATAACGTTGTGTAGAAAATTTATAACCATTAAATTTGATTTTTAAATATCCAAATTCGTTTTTTATTGTTATAAACTTTTTTTCTTGACTATGCAATATACCAAACATTTGCAAATATTTAACTCCAAAATAATTGGCAACAGCCTTATTCATTAAATATATTTTGCTATTTGATCAGTTAAATTTTGCATAATATGCATTTTGTAAAATCACTTTTCTTGAGTTTATTTTACAATGCAATTTTTTAGCATTTAGTTGCTTTGATTTATGACTATTTATTACATATAAAATAGCATTACTCAAACCATTAAGAAGAAAGATTTTTAAGGCATCATAGCCATTTAAAATCCAGTAATTTTCCTTAGGGTTTTTTAGTACTATTTTTTTTAATTTTAGAAGTTTATATTTTTTATAAAACTCACCATCTAAAACTTTTATTTCAATAAGTCTTTTTATAGCCGATTTTATAGAAGTTTTTTTAAACCCTAAATTTATTAGATCAATGATTTTGATTTTTTTATTTTTAAAGGATTTAATAACCATAAAAACTTCAAGCAACACTTTATTTGTTTTTAGCTTTTGATAATAACTTTCACTAATAACTATTTTTGATTTTAAAATTTTGCTTATAGCTATGTCGCTTAATTTGTCATATATTTTTTTACTATAATTTCTCCTCCAATTTAAATTTCTTTCATATGCATCCACAAAGGCATAATCATTGTTTAATTTAGTCTCTTGCTGCATTACCATAAATTCACTATAAGCACCCATAAAATTCCTTTCAAAAATTTTTAAGTTTTTTAAAAAAAACGAAGAGCACGTGGTATAATTTATGTAGATGTTTGAGGAGCTCAGAGTATTTGTGAGCTCTTCGTTTTTTTTAACTTATTTTTTTATGAATGATAGAAAAAAAGCAAGACCTTTACGATCTTGCTTTTTTTCCTTATTTGGAATTAACCCCACATTTTTCTACGCTTCCAAATTTTTTCGAAATTTCTTTTTAATTTTAGTTGTTTTTTAAATTGTTAACATTATAAACATAGCTTATTATATTTATTAATTTAACAAAGCAAACCAATAACACCTAACATTAAACAAAAACATTTTTATATATTATTTAATATATAATTAAAATATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGTAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAAGATTCAATTGAGATTGTTAAAAAAGTTTTTAGTGGTAAAGTCGGAAAAGATAAGACAATTACTTACGGAAATATCATTGATAATATAACAGGAAAAATCGTTGATGAGGTATTAGTTAATTGGTTTATTGGTAATAAAAATTATACTGGAGAAAACACCGTTGAAATAAATGCTCATGGTGGAATTGTTGTAACAAACAAAATACTTAATCTATTAATTGCTAATGGTGCAAGGTTGGCAAACAGAGGAGAATTTACAAGGCGAGCATTTTTAAACGGAAAAATTTCGTTAGATAAAGCCGAAGCGATAAATAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATTAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAATCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATCAATGTTGCAATAGTAGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTCTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCTGGAACAACAAGAGACGTGGTAGAAGGTGAATTTCAGATTAATGGTTTTTTATTTAATTTGATTGATACAGCGGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATTGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATAAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATACCAGTAATGAATAAGCAAGATCTTTTAAATAAAGATCAATATCCTAAGAATATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGAGAATTAAAAAATGCGTTAGTAAAAAATTACTTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAACTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCACTAGAATCACTAAGTCAAGGTTTAGGCCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGAGGGTCATTGAGCGAAATACTAAATAAAAAGCACGATAATGAAGCTCTACTAGATAATATATTTAGCAAATTTTGTTTAGGAAAATAATATGTATAAAATAAATGACAAAATAGTTGTAAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTAAGAAGTGATAAACAAACACTTTTTGTTGAAAATCTCTTATCTGGAGAAGAAGCAGAAGTAGCCATTTATAAATCAAATTCAAAAATTGCTTATGGACGTACAGTTAATTTGATAAAAAAATCAAGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTGTTTGAAAGTGGTGCAGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTCAAAACATTGAACAAATTCAACCTAGTCCAAAAATATTAAATTACAGAAATAAAATAACCTTACATGTGAATTATAAAAACAATAATATTTATATTGGCTTTTATAAAAAAAACAGCAACAAACTAGTTATTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAAGAACTTATTAGTGCCCTAAAACAACAAATTTTATTAAAAGATAATTGAATAAAAGAACTTAAACTTAGACAAATTACATTGAGGCATAGTAATTGAAGCCATAATATTGAAGTTATTTTTATTATTGAAAATCCAAATAAAAAAGGTTTTGATTTATTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAATATATTAAAGATTCTAAAGATATATTCACTTCTTTGTATAAATCAATGCCACAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCAACAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCAGCATGTGAGTGTGCAAAAATTAACATAAACCAAAATTTGATTAATCCAAAACTAGCGGATATAACAAATAGTTCAGCACAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCGATAGTTATATTTGACCCACCAAGAACGGGTTTAACAAATAAAGAAATTGATATGCTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGAATCGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAAATGTTGTCACTTTTGAAAAAATAAAAATTAGGTTTAATTAAATTACCTAATTTTTATTTATAACTTTTTAGCATATTTATACCATTTTCTTGTACTTCAACGAATATTGAATAGCAATGTTCTTGTGAATGCATCTAATAATGTTGCTCCCGTCATCAAAAACATGAAGGTGGCAGGACTGAAATAATGATGTCCTAATTGAATATACACAACAAGATATACGGCGCTCAATTGAACACCTAGCATAGTTATTAAAGAAACTAAATTTGGCATTCAAACATCGTTGGCACCTCATAAAGCCCTTAGGGTTGTAAGGTTTCCTATATCCAGAATAGCTCTTAGCATCATAATTCCCATAACACCATACCCAACAGAATTAATTATTTGAATATCTATTGAATAAAGTCTTAGCAAAGGCCAGGTTAATGAAACTCCTAATATTGAAAATAATGACTGTGCATAGAAGCCAAATTTTAATGCACTATTTCCAAGTTCATGAGCAGCTTCAAAATTTTGTTTCCCTATTTCGTTTGCAACAATTGCGCTTGATACATAACCCAAGGCGATCATTACTGCATTAAATATGTCATAGGAACTATTTACAGCTCTTCACAAACCAATGAATTGTTCGTTCTTTCCTTCAAATCCCATCGGAATTGCTCTTGCAAGAAGATATTGTCCTATGAATCAAATTCCTGTTTCAAAAGCAATAGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTGTTTCATGTTTCTTTAATTGAAATTTTATTGTTTTTGGTTATAAAGTGATAGGAAATTGAATAATCAATTGTTAAATGAAATATTGCCCCCAAAATTGGTTCAAGTGCAACCATTATAAAAAGATCTTCATTTGAAATCTTTCAAGGTAAAAACATAATTAAATAAGTTCAACTTGCACGTACAAATATGCTGGTAATTAATCCAAATATTGAAAATTTATTTTTTTCAATAGATTGTAAACTGGCTGTAAAAATAACAGCTATTGAATATATGAATATATCGCAAGTGGTTATTCTTAAGAATTTTATAGCTATGGTTGTTTCGGAAGTTACTGAAACCTTAAAAGGATTTGTATTAACTAGGGTAATTCCATTTATAGTTCCACCGTATAGCACCTTATTAAATTGATTCATTCTAGCTAAATCATTTATAGTGATGTTGTGATTTTTCAATAATTCAATGTTTGATTTAAAAAGATCTACACCTTCCTTGTTTTTTCAACCAAAAAGAGGAATGTTTTTTGTTCCAGAAGCTTGTAAAACTATTGGTGCAAGTCCAAACATAATAAAATAGACACAAGTATTTATTATTAATGATACATAAACGGCAGACCACAAAGCTTTTGGTATGTCTCTTATTTTGCTTTGTCCAAAAAGATTTGAGCATAAGACTAATGTTCCGCTTTGGTAAATAATAGGAATAAAGGTTATCATTTTGTAAACAGTTAATATTTTTGCAATCATATAAAAATAAAAACCATTGTATGAACCATCGGCGTGATAGACTTTTATATAATGCGAAGAAGCAAGAGAAGAAACTACCGATACCAAAGCAACAAAAAGTGTTTGAAAAAAGATTGGTAAAGTCAATTTGAAAATATACTTAAAGTGCTTAACTTTAAATAATTCATTTGATTTTTCAATAAAAATACTTAAACGATTTGTATTTCTTTGTTTACTGTTTTCTTTCTTTCTCATAACTCGTTAATTCTAACATATAAATTAAAATCCATTTAAACAACTTTATTATAATGTAAAATTTATATAAATAACGCTAAAATTTGCAAGGAGATTTACATGGGATTTTGATCAAACTTAAAAGATAAATTATTTGGTACCAAAGAAGAAAGAATAGCCAAAAAACAAGCAAAAATTGAAGCAAAAGAACAAAGACAATTAGAAAAAGAATTAATTAAAAAAAATAAGTTAAATACTTATATTGCAGGTCTTTCAAAATCAAACTCTTCATTTAATGAGCATATAAAAGAATTGCAAAACAAGCACAATAAAATTGACGAAGAATTTTTTGAAGATTTAGAAGAAATGCTGATTATGTCTGATATTTCAATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAACGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGATAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATAATAGGTTAAATGTAATATTGGTTGTGGGTGTCAATGGTTCAGGAAAAACTACGTCAATATCAAAAATTGCTAAGAAATTAATTGACGAGGGTAAAAAAGTGTTGATTGCTGCAGGTGATACATTTAGAGCAGCGGCAGTTGAACAATTAGAGATTTGAGCAAAAAGAGTTGGGGCTGATATTGTAACACCAAACGAAAATGAAGTGGATCCAGCGGCTGTTGTTTATAGAGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCAGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATTAATAGGGTTTTAGCCTCTAAAATTCCCGATGCGCCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCTAGGGTATTCAAGGAATCTACACCATTGACTGGAATCATTCTAACAAAAATGGATGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGCGAAAAAGTTGAAGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTAACAAAAGGTATTAACGAATAATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTTTATATGAAGATTTGTCATACGGGGAATTAGCCCAAGAATTAGCAATGTCTAGACCAGGGGCTTACGACGCGGTTAAAAAAGCAAAATTACACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAAAAAATTTTCTATTTTTTGTTATTAAACAAATAATTTATGAAAATAATTAAATTTAATATATAAAATATATATAAATTATTAACCAATAAAGAGGTAAGAATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGTTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTAGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTTGCGATTGTTGTCGGTGGCGGTAATTTTTGAAGAGGAACAAGTGCTGAAAAAAATGGTATGCAAAGGGTTAGAGCAGATTATATTGGTATGTTAGCCACAACAATGAACGCGCTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCAAGCCTAACAATGGATCCAAAGGTTTGTGAAGTTTATATAAATGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTGATTTTTGCAGGAGGAACTGGACGTCCATTTTTTACAACCGATACAGCCTCAACATTATTTGCTAGTGAAATTGATGCAGATGCAATATTGATGGGAAAAAACAACGTTGATGGGGTATATGATAGCGATCCAAAATTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTACTAGAAAAGGAATTGAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAGGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAATGGAATTAGACAGTTATCTTTTAGAACTAGATGAAGAAATTAGCAAATCAATTACTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGGGCAAATCCTGTCTTAATCAGTAAAATTAAATTAGAATATTATGGTTCACCAATTTCGGTAGAAGAAATAGCATCTATAACTACATCAGGACCATTACAATTAATTGTTAAGCCATATGATATGAGTGTGCTAAAAAATATTGAAAAAGCAATCATGGATAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATCAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTAACAATATAAAATGCTTCGGCATTTTTATTTTTAATTAATCTTTTATTTATTTATAATTATTAAATAATGAAAAACCTAAAAATTAGAGCAAAATCTGCAATTATATTATTTAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGGGTGGCAATTTATATGTTTTTTAGCGCGTGGGCTTTGTATGAAGTTATTTCACACAACCAAAACAAGAGATCTGTTAATTTGGTTATATCCTTACTTTCAACATTAATTTGAATAATACCAATTAATTGATTTTTAAAAGATTGAGGAACATTTTGAGAAATGTATAGCAATGGGCATTGAGTCTATGGCCTTGATAGAGATACATTGATTTCAATAATAAAAAACACAATATTTTTTAAATCAAATGCCTGGTCTGGTGCTCCGGGATTAGGTTTAATATTGACATTTTTAATAACAACATTCTTACTTATTGTTGATGTAAAGAGTCAAAAAAATGCTAAGAATTGGTTTAAGAACTATTTCATTACAATATTCGCTGTTATATTTATTCCATCAAGTTTTAAACTAATTTTTATGTATAACGTAATGAGTTTGTATTGACTATTCATATTATTTTTAATTCCTATTGTGTGTGATAGTTTTGCTTATTTAATTGGTAGACTTATAGGAAGGAAAATAATTAAGTTTGGCTTTGCACCTCACATAAGCCCTAAGAAAAGTTGAGAAGGAGCCATTGCCGGATATTTTATAGGGACCTTGTTTGTCTTTATATTTATGGGAATTAATTTTGGAAACTCTGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTGCGGCCGTTTTTGTTCTTCCTGCAATTTCTATTATTGGCGACTTAGTATTTTCAGGAATAAAAAGAGCTCAAAATATTAAAGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGACAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAAGTAAAATAAAATAAAAAAATCGTTATTACTTTTTAACGATTTTTTTATTAATATTAATATAATTAATAATAAAACTATTTAATGTAAATGAGGGGATAATTTATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAGCAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAGCCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTGAAATATAATTATTCATGTATAACTACTTCAACAATTGAAATAGACGAAAAAACATTAACACTTTCCTTGCTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGGTTGAACAATAAAAAAAGCGTTGCAAGCAAAAAATTAGATGGTTGAATTGAACAATTTAACAATTTAAAAGATAACGAAAATAACTTAAAAGAAAGCTTTTCAAAATCTAATTTTTATTCTAAAAAAGAATCAGCTAGACCAATTGAGATGGATTTAACTACGGCAAACGAAACGTTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTCATTGAAAGCAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTGTTAAAAAATTTTATGATTCCGAAAACGAAATACCTGATATAAAGGTGGGGGACACGATTATTGTTACCGGAAAATTGAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAGCTTATTGAAGATAATGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAACATGAGTGCTCAAGATGGAATTTCAACGCCCACCGACTATATTAATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCCGAATTTTATAATTATGCCAAAAAGCAAAATGATGTTAAGCCTATATACGGCGCAACATTAAGCACCGTTGATTCAGATAAAAAATTCTTCTACAACTTTAAAAATTTCAACTTAAAAGAAGAAGAATACGTTGTATTTGACCTTGAAACAACAGGATTGAGTCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTGCAAACGAAACGTGAACTATCAGCCTTTACTGTTGAAAGCAGCCACATTAATAATGAAATGTTAAAGAACGGTTATGCACAAGATGAAGGCATTAAAAAGATTTATGAAATATTAAAGGATAAAATTGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTAACAAACTCGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTAGCCTTATCTTATTTTTTAAATCCCGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTATTTATGATACAACTGTTGCCCACCGTGGTGATTACGACGCTAATGTTTTGGCTAAGATTTGAATAAAAATGATTGACAAACTTGCAAAAGATTACAATATCTTAAACTCACAGGATTTAAACAATGTCGATAGCCAAAAAATACTTTCTCATAAATTTACATACGAAACAAGAATATTAGTAAAAAATCAAGCTGGCCTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCTCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGCAATGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGAGATCAAGTTTTCACTGGAACAAATGAAAATTTGCTAAATTCACTAAAATATTATGACTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGTAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCGGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAAATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGATAACTCACCTCAAAAATTAAGAGAAGTTGTTTATAGGACTTTAAAAGAAAGATACGGGGATAATCCTGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAATCCAATTATAAAGTATGGATATTCTGTTATTTATTGAATAAGTCATAAATTAGTTAAAAGAAGTAATGAAGATGGTTATCTAGTAGGCAGTAGAGGAAGCGTTGGTAGCTCAATTGTTGCCAATCTAGCGGGTATTTCAGAAGTAAACCCCCTACCTCCACATTATTTATGCAAAAATTGTAAATATTTTGAATGAAATAGTAATAAAGAAATTTATTCAGGTTGAGATTTGCCAGACAAGCATTGTCCAAATTGCAACTCAATATTGTCAAAAGATGGGCATAATATCCCTTTTGAAACATTCTTGGGATTTGAAGCGGATAAAGTTCCTGATATTGATTTGAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAAGAATTGTTTGGAGAAGCGCATACATATAGAGCTGGTACAATAGCAACCGTAGCAGAAAAAACAGCATATGGATTTTGCAAAAAATATGAAGAAGAAAAGAGAGGAAATTATCAACCTTGAAGTCATTTGTTCTTGGACTTTTTAGCATCTAAAACACAAGGAGTAAAGAGAACAACTGGACAACATCCAGGAGGAATTATTATTATTCCTAAAGAATTCGATGTTGAGGATTTTACGCCTGTAAACTACCCTGCCAACGACACAGAAAGTTCTTGAAAAACAACACACTTCGACTTCACTTCTATTCATGATAATGTCTTAAAATTAGATTTGTTGGGTCATGATGATCCTACCGTTATTAGAATGCTTGAAACATTGACAAATACAAACGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTTTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGGGGCGAAACAACTGGAGCTTATGGGTTGCCTGAATTTGGAACAAACTTTGTAAGAAAAATGTTAAAAAAAGCTCAACCGAGATCGTTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGAACGGACAATGCCGAAGACCTTGTAGCAAAAGGAAAAAAATTAATAGAATGTGTATGTTGTCGTGATGATATAATGCGTGGGTTGATTGAATGAAATATTGAGCCCTTAACAGCTTTTCAAATTATGGAAAAAGTTAGAAAAGGAAAAGGCTTAACGCCTGAGTGAGAAGAACTCTTAAAAAGTCATAACGTTCCTGAATGATATATAGAGTCGTTACAAAAAATTAAATACATGTTCCCTAAGGCCCATGCTACTGCGTATGTAATTATGGCTTGAAGAATTGCTTGATATAAGTTATATTATCCATTACCTTTTTATGCATCGTTTTATACAAACAGGCCAGATGCTATTGATATTCAAATAATGAGTAGTGGCGCAGCAAGCGTTCACCAAAAACTCATTGAATTAAAAAATCGCCAAAATTCAACTGGTGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATATGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCCTTGGCTGATAAATGAATAATAGATGAAAAAGATAAGGCGCTAATTCCTCCATTTAATGTTGTGGATGGTTTAGGAATTACTGTTGCTCAAACAATAGTATCTGCAAGAAAAGAAGGCTTTTTCCTTTCAATTGAAGACCTTGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCGGAATTAAAAAAAATAGGCGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAAAAATATCTTAAAATAAAAAGAAAATGAAAATTTTAATAAATTAAAAGTGATATAATAAAAGAGCTCCAATTGGAGCATAAAGATCATTGTTTATTTAAATATATAGTTCGTTTATGTCAAATTAACTAAGATTTATTTGTTAATCTTTATATAAAAGTATAAGTTCAATAATTAAAAGTTTTATATGTTTTTCAACCTCCTTCATATTTTTTATCTATATACTTAAAACATAAAAAATGTGCGTATTTTAATTCAAAGAAATCTTATACAAAAAAATTTTTGTGTAGTTGCTTTAATTTGTTTTTGTTGTCTTATTGAATATATTTTAGGTTTTTGTAAATCCTTTCTTAAAAATGTTATTTCGTATTTGTGTGTTTGCAAAACCAAAGGGGTAAATTTATATTTAATAATATTTTTCTTCAAAAGCATCTATACAAAAATAAAGCTGTTTAAAAGTTTATATTTTTTATTAAAAAAATGTGTAGTTATTTCTTAAACAACGTCAGATAATAAGGCGTTGTTTTCATTTGCCTATTTTTTGTTTTTTAAAAAATGCTATATTTGGTAAAATTTTAATTAAATTAAATAATTATTTATATAAGAATGGAGCGTATTATGTCAAATAATGACAAGTGAAAAAATAAGAAAGTTAATTTAAAGAATTATGTTGTAGAATCAGGAAAGCCAAAAAGAGAATTGTCACGTTCATGAAAAATAGCTCTAACGGGTTTATTTTTAATTGTCATTCCTTCTTTTATAATGTTTTTAATTTTAGGCATAGATGGTTGAATTATAAAATCAACAAAAAATTTATCAAGATGAGGTGTTGAGTTTCCAATAGCATTAGCAATTGCTGCAATACAAATTATTATTGTTTTATTGTTAGTTTTTAAATTTAAGGTATTTAACACTGAAGCTTTAACCTTTTTAATTCCAATTTCTTTAGCCATTAATTCATTTTTGGTATCTAGTGGTCAAAGACCGGAAGATTGATATATTAGGGTTTTACCCGCTGTAGGTTTGGTATTCCTTGCAATTCCGATAATATTAATAAACAAGGCAGTTGCTAAAAGCCAAGAAAAACAAAGAAAAATTAAATTACTTGAAGAAGAACAAAAAAATAAATCATTACTTGACTAAACTAAACTTCGAGTTTAGTTTTTATATTTAAGGAGTTTAGATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGGCACTTATCACACGGATATAAAATTTCATTTAGTGGTAAATTTTACGAAGCTCATACTTATTCTGTTAATGATGAAGGTGTATTGGATTACGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGCGATGAAGTAGGTGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCACCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATCTTTAAAAGAGTTAATAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGCTTTAGAGAAGCATTACAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAACTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAACACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTTTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACCAATTCATTTCCAATTAAACGTTCTCATTTAGTTCGTTAGAAGTAGGCATATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACCAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAATGAAAATTATTACAGGAAAAGAGTGAAATAAAGCCGTATATAATGGTTCGATAAACATAAATAATAAGAAAAACGCAATTAACGCTTTAAATGTTTTCCCTGTACCTGATGGTGATACAGGAAGCAATATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCTGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCATCTAAAAAGTCTGCTAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGTGTTACTGATTCAGGTGGTGAAGGTTTATATGCAATATTGCAGGGAATGAGCGCATACTTTAGTGGTAATAAAGTAGAACCTATTGTTGCAGAAGAGTCGGTTGAAAAATTTATTTCTGATGAAGAGGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTAGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTGGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTCGTAAAAATTAAAATAGATAACATGACTTTACAAGCAAACAATAGTAAGAAAACTGCAAAGAAAAAACAAACCGAAGCGCCAAGCAATGTTGAATTAAAAAATAGCGCCATTGTTTCATGCAATACAGGCCAAGGGATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGCGGTCAAACAAATAATCCTTCAATTCAAGATATTGTTAATGCAATAAATGCAGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGCACAATTATTAGAGATAAGAATGTAATTATCATTCCAACTAGAAGTCAAGTCCAAGGATTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCACGAATTAATGAGCAATAGTTTAAAACATATAAGATATGGAGAAATTGCTGCCGCTATAAAAGATGCTAAATTAAACGGAATAAAGGTTAAAGCATCCGATTATATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTTTCGGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAATGATGCGCTAGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGTGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAGGAAAAAACAATGAAAAAAATAGTATTTGATTTATTGAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTGCAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAAAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTGAGAACTCAAAATTCAATGTTGGAAGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGTGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCTATTCTGCCAACAACAACGAATAAGAAAATGTTATTAATAGATGCAGGGGCAAACATTGAAATCAAACCAGAATATTTAAGTCAATGGGCTATGATAGCAAGTGAATGATATTCAATATTGTTCGGAAGCAAAAAGCCAATAGTAGGACAATTAAATGTAGGCGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGTACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGAAAAACAAAATTAGCGGCATTAATTTTAAAAAGGCATCTAAGGGAATTTAAACAAAAATTTGATTATAGAAATACAGGCGGAGCGTTTATTATTGGGCTTGAAAAAATTGTTGTAAAGGCTCATGGGGGAAGCGACGAATTAGCATTTTATAACGCCCTTAACCAAATAAAAATAGGTATAGAAAACAATATTTCAACAATTTTAAATAAGAAATTCAAAGAGGCTGATAATGAAGATTAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAAAATTAGATGAACAAAAAAGACGTTATTTTTGTAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTCATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCGACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTCTTATCGAGATATTATGGTTTTCCAAACTGTTTGAGGACAGGCAAAAGTGCATTTGCAAACGGAAAAGGAATTAAATCAGATTCAGATCTAATTGAATCATTCATTGGTGCTTGTTATAAAGCCTGCGGCTTGAAATTTACTAAAGAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGAGGTTGTAGGCCTAGATATGAAGACGAAGCCTTAAATAACGGCAAATTTGAAAGTAAGGTCTTTATTAATAATAACTTATATGGTGAAGGTATAGGTCAAAGTAAAAAAGAATCCCAAACAAAAGCAGCAAGTGATGCTTTAAAAAAAATGGCAAAAATTTAATTTGGAAGGAAAGAAGTATTTATGAAGTTAATTCAAGTTGAAGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCGATAATAGGGCCTAATGGGTCAGGAAAATCAAACATAAATGACGCAATCAGATGGGTGTTGGGTGAAACTAGTTCCAAAGTATTGCGTGGAGATACAATGGAAGACGTTATTTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACAAGGGGAAATGGTTCAAACGAATATTACATTAATGGTGAATTAGCAAGGCAAAGAGACATTAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCAAGTTTAGCAATCATAGGTCAAGGAACTATTTCCAACATTGCCGAATCAACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCTGGAACATCAATGTACAAAAGTAAGAAGATTGAAGCGCAAAGAAAATTAGAAAAGACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAGCAATTAAAAGAAGTCGAAGTTGCACTATTGGTTCATGATTTCATGGATTATTCAGATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATCTAATTAAAGAAGAATTAGAAACTAAAATAAGGGTATACAACGAAGCAAGTGAACAAAAAAGCAAGTTTGTTTTGGAATTAGAAAATAACATTAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACCCTAGAAATAAGAAACGCAAAAGAAGCAAAGCACCGCGAAATGCTTTTGAGTGGCGAACTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTAATATTTTAGGGTATAAGACATTCCTAGAAACTAAAAAGAAGGAATCTGAACAATTATCTCAATCTGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCCTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAGACAAATTTGGCTAAAGGAACACATAATATTGTTGAAAACTCTCATTTATTTAGAGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAAATACGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGAGGTAGAGCAACTTTTATTCCTTTATCATCAATTAAACCAAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCAACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTTTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCTAAATTGCTAGAAACTCGTTACATGGTCGTAACGCTAGATGGCGACATCATAAGAGTTGGTGGAGTTCTTTCAGGAGGACAAGCAAGTCAAAACGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCGAAAATTTCACAATTATCAAATGAGTTGTTTGAATTAGAAAAAGACCAAAATAAAGAAACATCATTATTAACAAATATTTCTCTTGAAAGAGCAAAGATTGACAACTTATATAGAACAACATTGGATAAGTTTGATTCATTACGTGAACAATATCGTAGTGCTTCGCAAGAAGAATATGAGGCAGAAAACAATAAAAAAATTGATATAGTAGCAGCAATTCAACAATTAATGCTTGAAAGAAGTGATTTATCAGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTAATTAAAGATAATGCAAACAAAATAGCAGAAAAAGCAAAGGCAGAAACAATTATTCAAAATGCAAAGCAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTATAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTGGATATAAGAGAGTTAGGACATGTAAACCTAGATTCAATTAATCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCGGCAAAAACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACACAAACAGTTACATTAGTCAATGCTGAATTCAAGCATGTGTTTGCAAAAATGTTTGGCGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGCGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGACGAAGTTGAGGCAGCCCTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTGCACACTCTAAAAAAAGATACCCAATTTATAGTGATTACTCACCGCCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGTGCAACTATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGATGCAAAGAAAACTATTGAAAACAACAATTAATTTTTTTAAAAAACGTTAGCTTAAAAAAGAGCTAGCGTTTTTTAAAATTTTACTTAAATAAAAAATTTACCGAAAGGTTATATTTTTTGATTTTTTTAAAAAATTATTACAAAATAAGGCCTTTTTTTTTTTTTTTAAGTTTCTAATTTTTAATATACAAAAATATACAAACATTATATTTCTAATATTTAAATTTATCCTTAATTTAAATTAACAATTAAAAAAGAGGTAAGAATGAAAAAGTCTAAGAAAATATTATTAACAATAGGAATTGCATTTCCACTTTTAGCTTCAACTCCATTAGTTGCTGCTGGATGTGTAGAAACAAAGAAGCCAGAAGGTGAAAAACCAGGAACAACTCCTGAAGTTAAGCCTGAAGGTGAAACCACAATAGAAAATATCATTAATAAACATAAAGAAATTAAAGATGATGAATTAAACAATACTAAATCATTATTAGATCAATTTAAAAAATCAATTGAAGAAAGAACAAATCAAGCAGCATTAAACGAAGCAAAGAAACAATTAAATTCAGCTTTAAAACATGCAGCAAAAATAAACACAGGGTCGTTTGATTTTAACCAAAAATTTAGTTTTAATAAAGAATATGTGAAGGGATTAGAAGCAATTAAAGGTAACGATTTGAATGCTATTAAAGAAATTACATTTAAATTAAATAGTTTTATAAATTTAGCGACATCTAAAAAACCAGGAACAACTCCTGAAGTTAAGCCAGAAGGTGAAACCACAGCAGCATTAAACGAAGCAAAGAAACAATTAAATTCAGCTTTAAAACATGCAGCAAAAATAAACACAGGGTCGTTTGATTTTAACCAAAAATTTAGTTTTAATAAAGAATATGTGAAGGGATTAGAAGCAATTAAAGGTAACGATTTGAATGCTATTAAAGAAATTACATTCAAATTAAATAGTTTTATAAATTTAGCGACATCTAAAAAACCAGGAACAACTCCTGAAGTTAAGCCAGAAGGTGAAACCACAGCAGCATTAAACGAAGCAAAGAAACAATTAAATTCAGCTTTAAAACATGCAGCAAAAATAAACACAGGGTCGTTTGATTTTAACCAAAAATTTAGTTTTAATAAAGAATATGTGAAGGGATTAGAAGCAATTAAAGGTAACGATTTGAATGCTATTAAAGAAATTACATTCAAATTAAATAGTTTTACCTATAAATTTAGCAACATCACAAAAACCAAAAACAGCAATAAAAATTAAAAACGATGTCGAAGGCGGAGTTCATGAATCAAATGATTTAATAGTAAAATTAGATGATTTTGTTTCTGATTATGTTGAAGAAACATTTAATGACATAGATGAAGCAGGTTTAATTAGTTCAATAGATAATTGAATCGGCATGATTAAGATTTTATTGAAGAATCTAAAAATCCTTCTGAATCGCTATATTTTAAATTAAAAATATTTGAAACAAAAAGGTAACAAAAAACCTTTTTGTTTTTATTAGTTAATTTTTAGTAGTTTAAATATAAAGGAATTTTGTTAATATTTTTGTTATTAAAACCTAAAAACGCAATATTTTCAATATTTTTTAGTTAAAAGTTAGATAATTTTTATAGTGTATTGTTAAACAAATACCATTTATGTCAATGACTAATGTTATATTACAATAAACAAGGAGAGCCTTATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGATTATCAAATAGCTTTTATTTCTCGAGGTCAATTAATAATTTATTACAAGTCTATAAAGCAATAGAAGAATTAAAGACAATTTCGTGATTTATTAAATCACTAGTTAGCCTACATATTTTTAAAATGGAAGATTTATCAGATTTTACTGAATATTTAACAGGCGAAAGCGAAAAAATAGAAGAATAATTAAAAAAACTTGTAATGTATTAAATTGCAAGTTTTTTTAATTAATTGTAGTTAATCATTTGAATTAAAATTAAATAAAAGATAGCAAAAGATCTCAAATGAACAATATTTTTTATAGTTAGTTTGTTGGAATATTTTAATATTTAAAAAGTAGCAATTGTGCTACTTTTTAAAATTGATAGATTATTAGCTTAATTTATATAGATGATTTGGGTTTTTCTTCAAAGCATCTTCTAAAAATGATTTAATTTCATTGTTTTCAATTTTAGAAATAAGAGTTTTAGCAGCTGATTCCCCTGCACCAATAAAGTCAAATGATTCAGGACTAAATGATTTCATTGTTGAAGGAATTTCTATTGTTTTTATTTGATTATCTGCCAAGGCTCCACTTTCAACTAATTCAATGTTTTTTAAGTTTATGTTTGTAAGCTTATTTGAAGCAAAAACACCTTGTGTAAGAGTTTTTAAATAAGGTAAATCTTCTAAATTTACAGATGTAATGCCACTGTTTGCAAAAGCTTTTTGTCCTAATTTATTTAAAGATGCAGGCAATTGTAATTTCTTAATATTGGTATTTGAAAAAGCTTCAGCATCTATTTGAGATAAGTCGTTGCATTGAGAAAAATCTACTTCTTCTAGGTTACTTAATCCATAAAAAGCACCTGCGCCAATTGATTTTAAGGTTTTTGGCAAAATAATTTTTCTAATATTTATTTTTTTGTTGTTATTTGAAGAATTAGTTGTAACTAATGTTTTTAATTCGGTTGGTAAGCCGGCACCATCGTCTGCTAAATTTTGCATAACTAAATAAGAAAATGCACCTGCGGGTATTGAAGTTATATTGGTTTTTGATAAATCTAGCAACCCTGAATCAGCAAATCAAGTTTGATTGTGATTGGTTGAGTTCTTAGGGTCTTTTAGCGGTCCTTGTACATAGTTTAGAGCAAAAGCATTGAACTTTTCTCCTCCACAAGATATTGCTGTTAATCCAGCAATAAATGGAGCAACTAAACCAAAGCCTAAAAATAAAAATTTTTTGTTCTTTTTCATAGTCAAAATTTTACCATAATTTTGTTATTTTAGTTTTTTATATTAAATGTCAAAAATAAATTACTTAATTTATATAATTAAATTAATAAAATCATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCAGCCGGCGACCAACCTCAGGCAATAAATGAGTTGGTTCACGGTGTTTTAGAAGGAAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACTATGGCCAATGTCATTAATCAGTTAAATAGGCCTACATTAGTTATAAGTCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCTGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCATACATTCCTAAGTCTGATTTATATATTGAAAAAACAAGTAGAAACAATAAAGATTTAGAAGCAATGAGAATGTCTGCTCTTAACGCCTTAAGTATTCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCTGAATTTAACCCAAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTACGCGATCTTTTAACTAAATTGGTTAATTTAGGTTATGAAAAACACAATGGTGATTTATCTTCTGGACAATTTTTTAATAAAGGGGAAGTTATTGAAATTTGTCCCGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTTCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAACGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGTGAATTGAAAGAAAGAGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCCCAAAGGATTCATGATAAAACTTTAAACGATATTGATTCATTAAAGGAATTTGGCTATGTTAAAGGCATTGAAAATTACGCTCGATATGTTGATAAACGTGAACCCGGACAAAAACCATACACAATATTCGATTATTTACCAAAGGATGCAGTAATTTTTGTTGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTACGAAGGAGATCGTTCTAGAAAAGAAACATTGATTAAATATGGTTTTAGATTACCTTCAGCCTTGGATAATAGACCTTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACTCCTGGCGACTATGAAATAAATAAGGCAAATGGTGAACTAGTTACTCAATACATTAGACCGACAGGCCTTTTAGATCCCATTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGACTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTGACTACAACCAAAAAGAATGCCGAAGAGCTTTCTTTATACCTTTCTGAATTTAAAATTAAAAGTGCATATATTCATGATGGATTTAAAACCTTTGAGAGAAATGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTCAGTTTGATTTGTGTATTAAATGCAGATTCTATGGGGCTAATGCGTGATGCTAGAAGTTTGATTCAAATAGTTGGTCGAGCCTCTAGAAATGATCATGGAAAAGTAATATTTTATGCCGATAATATTACAACCAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGAACATTACAAATTAAATATAATAAAGAACACAACATCACTCCTAAAAGTATTATTAAACCAATACCCGCGCCTATTCAAGGTGGTATTTTATTAGAAGATAATAAAGCAATAAAAGAAGCCAATAGGACTATTCATAATAAAAAATACATTGATAATATGATTAAACATATGAAAGAACTAGCGAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGAGATTATTTGATTGAATTAGGAGTCGAATTTGATGATTAAAAAAAGACTTTGTTGTCTTTTTTTTAATCATTTTTTTGTTGCTTAAAAACACAAAAAAATAAAAAAAGAGTTATTTTTTCTTCAATATTGAAAACTGTTTCTAATAAAAGAAAGATTTGGAATAATGGATATATTGCTAATAAAGTATTGCCAAATGTCAAATATACTGGTGACACTCTTAAAGTTTTTGAATCTAGATATAAAGCAGTTAGAAAATCATTTGCCGAATTGGAAGAGGTACTAAAGAATAATCAATAAAAGTCAGAGGTTTTCTTAGAAAACATTTTAAATTATCTACCAGAATCTGAGTTTGATTTAGTTGTGACTTCTCCGACGTATCCATTTGCTACTTATTTTGTTAAATACAATAGGTTGACTTATTATTAGTTTGGCTGGGTGTTGATACTAAGGCTGTAGAAGAGCAGAATCACGTACTAAAAGAAGCAGAGAAATGCTAGTGAGCAATTTTTGAAAAAATGGAGATTATATAAGCATATTCTCTCGCTGATTAAGAAAAAGGATATTTTTGTATGGCTGTTGTGGATACATACAGACAAAAGGAAAAAGTCTCTTTTGTTGAATGGCTATATGATATATTTCAAAGCAATGGTTCGGTTTTGTTTTCTGATGAAATCAGAGACATTCAAACTCAAACAATGACCCAAAAAAGAATTCAAAAAGAGCATAAATTGGTTTTTAAGAAGGCGGTTTAAATGGATAAAAAATTCATTTTTCAAAATATGATAATTTAAATGCTTTAGACATTGAAGATAAAACTTGTGGCTGTAGATGATATAATTTCAATATATGCAAGTATTATGAGATAAGTCTTGTGCATTTGGTAACAATGAAAAAATATGTTTAACTCTACCAAGAGGATGGGGAAAACTTTTTAAAGTTGAAGAGTGAGAGCAAAGAAAACAAGTAATTTTGTAAAATATTGAGGTAATTTTATGGATGAAAGAGTGATTGATAAATACAAGATACATTTTATTAATGATAAAAGATACTATGAGTTTGATATGACTAATTTATTACCATCTCTTGATGAAACTATCCCTTATTATTTTAAGTATGATGATATTGAGATATATTCTAATTCTTGGAATAGAATGACGCTTAGTATATTATCTGCTTTGGATAATAAAAATCATAAATCGAATGATGAATTATTGATGATACATTATTTTTGGACCAAAACAGATATATTCTCTTCTGAGAAAAGAACTAATTATACACCATTTAGAGATTTGTACTTGAATACAAATCATACATCTGCACATGCAATGATGAATATTCAAGGATTACTTAAAGCCTATAATATTCCATTAGAAAAATGTTACTTTTTAATAAGACGACATATTTCTGCTGAGCCAGAAGAAGTAAAAAAGGGCATAAGAGCTGATACTATTTTTGCATTTTCACGTTCTTTACAATTAAAAGGATATTCGAGTGATAGGATAGGAGTAATAGTTTCTAATTTTCGAACAATTAATGAGATATTATCTAAGGTAAGTCCAGGTTATAATGATTTTTTCTTATTTGATGATTACTATTATTTTACGAATTATAAAGCTAAACTAGTTGAGTGGTTAGAAAAAAGACATTATTCCGAACAAGATAAAACTTATAGGGCAGTTAAAAGATGTTTAGATTTATTAGGTGATTTTTATAAAAACAAAAATTTTTATAATGATCTTTCGAATACAATTATTACCAATGAGACAATCAAGTTTTTGGGTGATGAAATTGAAAACTTATTTTTATCATTAAACACTGATGTAATTGTATCTAATAAGTTATATGCAAGAATGAGAATGGTGCATTATGAACTACTTAAAAGCATTAACCAATTGAATAATCCCAAAAGCATTTATAAGTTAGCAAGTATCTATTTTGGAAAAAAATATTATTTTAAAGAACCTTTTATTAGCAAAGATAAAAGTGCAAACTTATCAAATGATGAAATAATTTATTCATACGCTTATACAATGGATGAGATTTCAATCTTAAAACTAAATCAATATGCTGATAAAATGCAGCTAAAAAAACTTGATAATTATCTTTTGCTTTTTGAAGATGCTTCAGATGAGTATATTCAAATTGATGAATCAAAACTAATAAAAAAAGATAAAATCGACATAGCTCCAGCTGTTTTAGATAAAATTGAAAAAGAATTATTATATTATCTTGATAGCTTTGGCAGTATAGATAGTGAAACATATGCTGGCTACAATTCAATGCCTTCATTAAATGTAAGTTGGAATAAATACTTATTATTAGGATTGTGTAGAACATATTTTAATGAATTAATTTCTATAAAATATAATCGCAAACAATATAAAAAAACTACCTTTAAATTAGAGTTAAAACAAAAATAAAACATCAAATTTACACAATAGTTGAAAAATATTACGAAAATATTGCAAAATTATTGTGTTTTTTTCTTTTTATTGACTATAATATAAGAGAAATCAATGAGAGGTATAAGATATGAAAAATAAAAATGATAATTTTAAACGTATATCTGAAAACAGAGTATCAAAGATTTTGGCTTTACTCTCTCAGTTAACGAATCTTAGTAATTCATCTTTTTATGAATATACTGATGAGGATATAGAGAAAATGTTTAATGTCATTTATGAAGAAGCAAAAAAATCAAAGGAAATATTGCTAAAGTCAAATGATAAAAAAAGAAAGAATAAAAGGTTTGAGTTATAGGAGGTAACAAATATGTCAGACAATAAGTGGAGATTCCCTGCAAGTAATCATGGTGAAAAAAAAGGTATTAGTAGTGGCGATACAGAAACATTTAAAAAGTCACCTTTCCAAGCGTTCGCGAGAGAAATCTTGCAAAACTCTATTGATGCTAGGTCGAGTGATGAAGAACCAACTAGAGTTGAATTTTATCTTTTCAAAATAAAAACAGATGATATTCCAGGCAAACAAGAATTATATGATGCTATAAGACGTTGTAAAGAATTTTGGTCTTATAAAAAGGATTATGTTGATGAGTATGATTCTATGATGAAGTTACTTGAATCAGAAAACATATGGTGTCTTCGAGTAAGCGATTTCAATACTACTGGCTTAATAGGCGTAGAAACCACTAAGCAAGAGAATAATAAGTTTTTAGCATTAGCAAAAGGAACTGGTGTTTCAGAAAAAAGTGGTGTAATGGCTGGCGGTTCAAAAGGTGTAGGCAAAAATGCAGCGTTTTTAATGTCTTCTTTGAGAACAGTTGTTTATTCTACTAGAAGTAATCAAGATATTGATGGTAATCCTGGAGTCTTTAAAGGCTCCATAGGTGTTGCTGAGCTTGTGTCTGGATATATTGATGATATTGCTATTGAGAATAGAGATTATACGCAAGGCACAGGATATTTTAGCTGCGATGATTTAAACGGCGCGATAGATAAAGTATATAATTTGGATCGTGATTTTACTTCAAGAAATAGTGAATATGGAACTGATATTTATATTATAGGGTTTAATAACGAAGATAATTGGCAAAAAGAAGTTGTTAATAGTTTGTTGGATTCATTTATGGCAACCATAGTCAGAGGTGAATTAGAAGTTAACATTAATGGAAAAGAAATAAATAGGGATACTATAAAGGAAATCGTATATGACAATTCTATTATCAAAAAAAGTAATCGTAGTAATGTGATTTCCCAATATCGATTATTAACTAATGAAGATGGAAAAGTATTGACATATGATATCGATACTGAATATGGAAATTGTGAATTATATATTTTACCATATGATAAATCTGAGGAAGATTTGGCTACTAATATGTGTGCGATGATTAGGTCCCCATTAATGAAGATTAAAGATGAAAAATTGGGACCCTGTTTTAGAGTTTCAGCTATGTGTATTATCAACGAAGGTACATTGGGTATTAAACTTAGAGATATAGAAAATGCACAACATACAAATTGGGAAGTAAACAGAATAAAAGATGTTTATGAAAGAAAAGAAATACAAAGTGTTATTGATAGTATAAAACAACAAATAAATGATAGTGTTATAGATTGCTTAAAATTGGGTGATAATGAACCCTTAGATCCTAATGGTGCAGGAGATTATTTACCTGATGTTGATAATGGTGGTGATTCAAGTTCTGGTGCTAATGGAAAGCAAAAACCAATTGAAAATGTATCTATCTCAAAACCAAAAACCAATAAGGTAGTAGAAAAAAATACTAACCAAGAAAATGACAACGGTGATGGTTTAGAACCTGATATTGGAGAAGTTGATGATAATATTGATGGTGATGTTCAACATCCTATTGGTACAAATGATAAATCAAATGAAGGAAGACACCCAGGAAGTGAATCTAGTGGTGAGCAAGAAGGCGAAAATATTGTATTTAAACGTGCTAAATTATCTGGTGTTAAATATAAAGTAATCTCTACTAATAAAAACGAAGGCAGATTAAGAATTATTTTTACGGCTCCAATTGATTTTGAAACTTGTTATTTGAATATTGCTTTATTAGATGATGCTAATAATTCTGTTGCAGTAGATATTGAAGAATTGAATTGTAATGGAGTTAATGTTTATGGTGAAGATAAAAAAGAATATGGCCCATTCAAAATAAGCCGTAATGATAAGATTATTTTAAATGTAAAGACTAATTTAAACGGCTATTTCGCTAGTGAGGTGAAAGTAATATGCAAGTAAAGAATAAAATATTTCCTTATCCTGTTTTAAATAATAATAAACTATATTCAAATTTTGGAGATTATTCATTTGAAATCATGTATGAATCTTCTGAGGATGAATATGCATATATTTTAAAAGATATTAAATTTTCAACTGAAAGTAAATTTATAAATGATTTGTACGATAAAAAATTGATTAAAGTTTTTTTAGTTGTAGAATGCTCTGATACTGTTTTTAGAAAAAAAATAGAAGTGGATAAGATATCTTCAACGATACGTCTTTATAAAAATGATTTTACAGAAAAGGTAGATATCTCGATGTTTGCAGTTGCAACAAAGAATTTTACAGTTTTTTCTAGTGAAGTTGACGAAGAGTATCAAGATATTGAATTTGAAATTGAAAAGTACGATATCTTATGTGCAAATGATGGATTTAATGTTAGATTTAAGCATGATGAATCAGAGAATAATTTAGTTAAATCAATTTTCTCAATTATTCCAAGCGATAGTATTAACGATGGTACATTTGAAGTTAATTGTGAGTTAGGAAAAAAGATTACTATTTCTATGTCTGACACTGATTTTAAAAATATGAATATAGTTAATACTGTTCCAACATATAAAGAAGTAGTATTTAATATGCTATTAGTTCCCGCATTAATTGAAGGTTTGACTTTGTGTTCGAAAACTGTTCAAGAAGGAACAGATGATTTAGATGATATTGGAAATAAATACGTCTGGTTTAGAAGTATATTAAAGTCATATAAACGTTTACATGGAAAAGATATTACAATTGATGAATTTAAAAGTTTATCGCCTGTTCTTTTAGCCCAACAATTACTAGGGAAACCACTAGGTGCAGCTTTACATAAGCTTGTTGTTGAAACAAATAAAATAGATGAAGGAGGAAATGATAATGAGTAAAATCAACATTAAAATGCTTTCAGAAGATGCTTTAGTATATTTAAAAGAAAACGCTGAAAGTATTGCAGAGAGAATTGCAGAAAATGAAGATAATTCTTGGATTAAAGATACCATTCCTCAATCTATACGACTATTTGTTGAAAAGAAATTACAAATTGAAGATTTTGATTTATTAGACAATCCTGAATCTGCAGATAAAAATATTGATTTTCAAAATTCTATTAAAATATACGAATCGTTAAATGAACTTCCTAGATATATTTTATGCGACCAAAGATTCTGGCTTTGGCTTCACTTGGATAAGTTTTACAAAACAGTTAGAAATATGATGAAGATCAGAGGAAAGTCTACATTTACAGACCATTGGCTTCAATCTGGAGGAACTAGAAGAGGATTGATGTTTGGTGTACTATCAAGATGCTATTTCAGAGTTGCTTTAACAGTTGATGAAGGTAAAGCTAATAAATATGAATTAACAAAATGGGTAATTGAAAATCCAGAGAGATATAGAAACCTTACATGGCGTTCTTTTTCATCAGAAGAACATTTAGTTAGAGGCGTTTTGAAGGGTGAAAAGAAGGCGATTGATGAAAACCCAAAAAAGGAAGCAAATAATCTTTATCCAGAAATTGCTAAATATGTATCTATTATTGGAAGCGTTAGGTTGTTAGATTACATATCTGAAGAAGATATAGAAAAGATGATTTATGATAAGATGATAGAGTATATTAATAGTAGTGGCGGAGATGAGAAGTAGATGAAACCTTTTGTAAAATGAGCAGGCGGAAAACGTCAAATTTTGAAAAGAATAAATGAGTTCATTAATGATTCTGTTGAACCTGGTTCAAGTTTTACATATTTAGAACCCTTTCTTGGTGGAGGGGCAGTATTCTTTGATAAAAGACCAAAGAAAGCTATCATTAATGATCTAAACGAAGATTTAATTAACGCCTATAGAGTTATTCAGTCTGATCAATATGAAGAGTTGATTAGACTTTTGGATGTGCACGCAGAAAACTATAGAAAAGAACCGGACGACTATTACTATGAGGTCAGAGCATGGGATAGAAATCCAACTAGATGGCCAAACGGTTATACAAATGTTCAAAGAGCTGCTCGTATGATTTTTTTGAATCGAACATGCTATAATGGATTATATAGGGTAAATAATAGGGGCGAATTTAATACACCTATTGGTAGATATCATAATCCTTTAATATGTGACAGAGAAAATTTAAAAGAAATTCATGATTATTTATCTTCAAAAGAAAATGAAATAACAATAATGAATGGCTCTTATGAAGAGTGTGTAAGATTAGCTAAAGATGGTGATGTTATTTATTTAGATCCTCCATATGATTATGAAGATGATGATGGCTTCACTAAATATCAAATGGCCGGTTTTTCATTTGAAAATTTCCGTGAATTAAAGAAGGAATGTGATAAAGCACTGAGTAAAGGTGCGTTTGTAATTATCAGTAATAATGCAACTCAAAAAGTTACAGAACTATTTGAGCAAGACCCTAAATATAAGATTTTTTATGATGTAAATAAGTTTTCAACATTAAGAATTATTAATTGTAATGGCGCTGAAAGAAGAACTGGAAGAGAAGCTATATTCTGGGGAATGGATAATAATATTCCTTTTCCACAGGCAAATGATATGACTAAAATTATAAAACTTGTTATGGCTGATGAATCTGTGATTAATGATAAGGCAAAAGCCATGGAATTAATCCATGTTAGCACGACTAGACAAGTAGCATATTATTTCTCTGCACTTCAATTTTTAAAGTATTTAAAGCATGATAAGACATTTACTGATAGAGCAAATAGTTTGATGAAAAATAAAAGCAAAATCATTATCGACATTTATGATCAATTATTAAATGATGATATGTTCGGTAATGCTTATAAACAATTTAAAGATTCAAAAAAGGTAAATGTTGAAGTTATTAAAAATGAAATAAGAAAAAATAAGACTAATTTGGCGGACTCAACTGTAAATAGAAGAGCATCAACTATTAAGGCATGGGTTGAATGGATGTATACAATTGATGGTCGTATGCGGACGATTAAATCTTAATTGTTTTCTAAATCTAAAGACTAATTTCTTTATAAGATATGATTTTTTCACGAAGATTTTAGCAGGTGATAAATATATGCGTTTAATAATAAAACAGAAGAGTTTATGCATAAATTATTTAGCAAATATGAGCATGTAATTAAAAAATCTCATTTTGTTAAACAAAAATATATGAAAAATTGGTCCCATGATAAAAATAGGATTCTAGCAAGTATGGATGGTGAGCCTTATTTTTTCACTACAACAGAAAACATATGCCAGAGCGTTATATGTATAAACTTGAACTTTTAGCAGACAATGAACACAAATTAGAAAATTTTATTTTAAACCGTCTAAAGTAGTTAGAGATATTAATAATAACATTCTTATTGAATGGCAACTTTAATTCGTGTTAATGAAAAATATTGAAGAAACTATTGAAATAAGAGAATATTTGATTGAATTAGGTGTTGAATTTGATGATTAACAAAAGACTTTTACGTCTTTTTTTAATCATTTTTTTGTTATTTAAAAACATAAAAAAATAAAATAAAAGTTATTTTTTTTATCAAATTTTTAGTTTTTTTAACCAAAGCGTTTTTTTTTTTTTTTTTTAAAAGAGCAAAATTATTTTTAAGACAAGTAAGGGAAGTGATGAAAAAAACCAATATAAAATGGAGTTTTTTTATACTATTAGCAATTATTTTTATAATTGCTTTTGCTTTAATGTTAACTAGCTGTGCCTCAACTGATCCTAGAGATGCACAGTTTTATATTAGGGATGATAGCTTTTATTACAATAGTAGCGCAAACATAACAAAAGTTAATTTTCAAGTAAGTTTTGAGAATAATTCAATATATAAAATTGTTAAGAATTCAATAACATTTGATTTGTATGATAATGGAAAATATGCATTTCAAAACTCTTTTAATTGAGATATAAACATAAAGCCCTATAGTACATTAACAAAAGAATTATCGTTTTCTGTTAATGGAAAAGTAGATAGGCTTGAAGTATTAAATTGAGAGATAGTTAACGCTAGCATATGGCAAAGTTATAAAGCTTATTTCATAACCATTATTGTTATAATTTCAATATTACTAGTTGTTTATGCAATAGCACAATTGGGTTATAACATTAACGAAGGATGTGACCCTGAAGATTCATTTGCGGTAATTATTTGAATTCTTACTAGTGGTTGATGAAGTTTTTTAATTGCAATAGGTGTTGCAATATTACCTTATTTAATTTATTCAGGTGTTTGAAAAACTTGAAGTTGAGTACCTCCAATAATCATAGTAGGAGGAGTAGTTGTATTAATAGCGGCTTGTTTTTTAGTAAGTTTTATATATTTTATTATATTTGCAATTCATAACAGAAGAGTCGAAAATCAAGCGATTAGTTATAATTGTGATGAAGTAATGAACAAATACACTAAAAATCAATTAAATCAAGTTTATAAACATGTGCTCTTAGATATATGCAAAGCTTCTGAATTTAAAGTTGATGAAAAGATAAAAAAAGAAGAATTAATAAATTTAATTTTGTCAAGAAGACCAGATTTTAAAGAAAGCGACAACACAAAAAATCTTTCTAAACCAAAAAACAATGAAATTAAACCATTATTAAAAAGCCCTCCTTCTAAAAAGCCGATGTTTGAAGACATTGTGGGTTTAAACAAAGCAAAAGAAGCGTTTATTGAAAAGATTATTCTTCCTTTCAAACACAAAGAAATATTTGAAAAGTTTAATAAAAAAATAGGTGGTGGCATATTATTATACGGTTTGCCCGGCACTGGAAAAACAATGTTTGCAGAGGCTACTTCTAATGAATTAAATGCTTTATTTATTCCAATAAAGTGTTCAGATATTAAAACTAAGTGATATGGTGAATCAGAACAAAAAATACATAAAATTTTTGAACGAGCAAGAAAGCAAGAAAAAGCAATAATTTTCTTTGATGAATTTGAAGCGATTGGAACTAAGAGAATGGACAATGCAATTAGTGAAGGCAACGACATAGTTCCACAATTGCTATCCGAAATGCAAGGAATTGATAATTTAAAAAATGAATCAAATATTATTGTTATAGCAGCAACAAATAAACCATGAATGATAGATTCGGCATTTTTAAGGCCAGGAAGATTTGATGAAAAAATTTATATTCCACTTCCTGACTACGAATCAAGAAAAAAGATGTTTGAAATTCAATTATCTTCGTTGCCAATTGCTGATAATTTCGATTTTGATTATTTAGCAAGCATAACTGAAAATTTTAATGGTGCCGATATTAAAGAAGTTTGTGAAAAAATAAAAATGAGTGCTATTAAAAAGAATATAAACAATAATCAAGAACATAAAATAGAAATGGAAGATGTAAAACAAATAGAAAAAGACATTAAATCTTCTGTTAATCTTCAAGATATTCAAAAATTAAAAGAATTTGAAAAAAATTTTTATTAAAAAATAATCTATATAAATTTTAAAAAGGAGAATATTATGCAGGTAAAAAATGCCAAAAAATTTGCGTTAGCTACTATAATATTATATTTTATTTGTGTAGTACTTTTAATACCAATGATTATATTAAATTTGATGCAAGATACAATAAAGGAAAATCTTTCTTTGTATTCGGCGATAGCTTTGCTTTCGTTTGCATTAGAATATTTAGGAGCCGCTATAACGTTCATAACGTTTTATATAATTACAATTGTAAATGGTTTTTATTATAAAGAAACGCTTGGATCAATATTAATGTGCGTAGGAATTATTGTGCCTATAGTAGGTATTATTGGAACATTTTTTGTTTATTTTAATCTTAAAAAAATACCGGTTGTTAAATTAACCACGCCAAACACTCAACCAACAGTTGGTAATCCAAATCCATCATGAAATGTCTCGGATAATAATGAAGACGAAGATATAGAAGAGGATGAATAAATGAAAAGAAATAAAATAAAAGAATCTCTAGCGATTGTTATATTGCTCCCTATTATTACTGCCTTGAACTTACTTTTTGTATTTACTAGAGGATGAATAATTGATTATAATGCATATCATAATACCATTCCTCTTTGATTAACTTTATTTTTAGCTTTAAATGTAGCTGTTTTAGTTCAATACATAGTTGTTATTGTTTATGGATTTAAATTGCCCAACAAAACAGGTGCAATATTAATGTGTACAGGAATTTTTTCATTTGTAATAGCATATGTTGGATGCATAATGGTTTATATTGAACTTAGGAACAATGAAGAATTAATTTAAAAATAAAAAATAGACTCGTAATTAAAAAGGAATTACGAGTCTATTTTAGAACCTCTTTATTAAAATTAGTAATGATTACCTCTTCGACATCACCTCGATTTTTACCATTTCTGTTAATGTTTCTTCTTGCTTTAATTATGTTAATATTAAAATCACTATATAAATTGCAAACAAAATCAGTTTTGTGATTACTTAATATTACATAAACGCCCTTTTTTGACAACTTTTTAAAAACATTAGCAAGTTTAATTTGCTCATTTTTTCCAAAGGTTTCATTTGAATAAGAAGTAAATGAATGTTTATTTTCTAAGGTATCATAAGGAGGGTCAAAATAAACCAAGTCGCCAGCTTTTGCACCTTTTATCGCGTCTTCAAAATCTAAATTTGTTATTTTGTAATCTACATTAGAAAAATATTTAAAAATAGAATCATAGTTTTCTTTATTATAGCAATTGACTTTTTCTTTCTTTCCTGATGGAACATTAAAAAACCATTTTTATTTACACGATATAAACCATTAAAACATGCTTTATTCAAATAAATAATTCTGGCAGCCTTTTCTTCATCTGACAAGTTTTTATATTCGGGTTGTCTATCTAAATCTCTTATTTGATAATAGTATTCGTTGGAATCCTTACTAGTATGAAGGTCTAAAAGTGATTTTAATTTATCAAATGATTCTTTCTTTGTAAAACACTTGTATGCTAGAACTAGTTCGCTGTTAATATCATTGATGTAAAAATTCTTAGGCAAGATACTAAAAGTAATGAACCCCCGCCAATAAATGGTTCATAGTAGTTATTAAATTTTTTAGGTATAAAATTTAATAATTTTTCTAAAATTTGTGTCTTGCCACCTGCTCATTTTACAAAAGGTTTTATCACTTTTTTCCTTCCTTAAAATTAATTATATCTTTTAAATTCAAAATTAACTTCATCATTATTATTTACTAAATTTAATGAGTAGATTAGATTCTTTGTAGTTTGCTTTTCTGTGTCGTATCATTCCATTGTTTTTCTAAAATTTGTTACATTGCGTTTGTTATTTTGTTGGTGGGTTCAATATCCGTTGTTTCCAAAGGTTAAAAAATAAGAATTGTATAAATCAATAATTTCGTTAATTAATAATTTTGCTTTGGGCGAATTAGTATTTTTTATTCCTTCTATCAACTGTACAACTTGTTCATGTAATGATTTATTTTCATCATATTCAATGTTTGCAAATAATGATAATTCCTTCATTTTTTTATCTAAAATATTTTTGCTTGAAACAATGCTTGATATTTCATCTGATTCAATTTTATTATTATTATTTTTATCAACCCAAAGTTTTAAATTTAAATAGTTATTTTGATTTGTATAATCTTTTATTTCATTAATTCCAATTGAATCAGAAAAATATGAAACATATTCATAATTTTTAACATTATTTGAATATATGCTTTCTTCATTTAGCAGATTCTTTGAATAAATTTCATTTCATCTGCTTTTGGCAATAAAGTTGTATTTTGAAGTATGAATTCTAAGTTTTAACGGATTTTTATTTTCTTTTTGGAATAGTAAATAAGTTGGATATTTTGATTTCTTGCTATTTGGATCTATTAATGAATTTTCTAAGTTTAGAAATCCTCCAATATTTATGTTTGAATATTGAGAGTTAATATTGCCATTATGATCAATATATCAACTGCTAATATCAGATCCCATATAACTAATAGGTTGACCAAAACCCATTAAATCAATATATGCTTTTAATAACGCCTTTACCTGTTGATTTGGTTTATAAATGTATTTTCCTTGCTTATTTCTAAACAAGAATCTATTATTAATATTTTTATAATCTTTTAATTCATCATCAAATACTCAATTGGCTGAATATATTACATATTTATTATAATAATTGGCAATCATTTTCAATGTGTCATCGCCAATTGATGATAGAAAAATATCATCATTTTTTAATTTATAATATAAGAAATTCAAAGAATCATCCAAAGTTAAATGGCCGTCAAACATTTGAAAATTTTCATGTGCTGAATACGAAAGTTTTACTAGTTCTCTTGCAAAAAGTTCCTCAAATGAAAACAAGTATTTTATTTGATCAATATTAGTAGAATTTGCATAATAAACTGTGTTGTTTGTTGAATTATTAAAATAAAAGTTTTTATTGTTTAAAATATTGTATTTTTCAACTTCTTTGTTTGTTAAGGCGAGGTTAGCTAGCTTGAATAAGTCATATTGACTAAATTTTGAATAAACTGGTATTTCTTTGTTTTTGGGATTAAATAATTCTTTATTGACTAAATATTCATCGTTTTGCATTGAACCATCGTAGTTTAGCGTTTTTCTGAATTCGGTTAAAAATTTGTTGTTATTGCCATTGAATTTATTGATTATTTTATTTAATTCAGGAGTTGCTAATTTATAGCTTAAATCATTATTGGCAAATTCATCATTTTCCCTTATACTTCGGTTGTATGTATTATCAATATGGTGTGCATATTCATGAAATAAAGTATTAAAAACTAGTTCAACTCTCTTTTGTAAACTTCATGATAGGACTTGTTTTATTCCCCCAGAGATAAAATGATTTATTTGAATAAAAATGTCAAAAGCTTGTGGCAAATAAACACCATTTACATCTTTAATATTCGAAACATTAGGTATTTTTTTATTTATATAAACATGATTTAAACCATTAATTTCAGGACCATAAAAGCCTCTTTTTAAAAACTCTAAATTCAATAATTTTCTTCCTTCTTCACCCAAAAAATAAATAGGTTCATTATTTTTTGTTAAAGCAAAAGGATATTCAAAAATACTTAAATTTCCATATTGATAATTCTTGTTCTTTAATTGAACATTGTTTTTGGCAAATGTTTTTGCATTGAAACTTTGTAGATATCTTTCTTCATTTGGCATTGAATAATTTTTATTTTTTTGGCCATCAAATAAACCCCCACCATTGCCATTTGAATAATTAACATAATTAAAAATGTAATATATTACGACGCCAATAATACCTGCCGTTAATATAAAAGCCAAGATTATAATAAATATTGCCCATTTTGGTAGAAATGCTTTTCTACCTTGTTTTTGCTTGCTCATTACTAAACTACGCTCCTAACTAATTTTAATAACTAATATTTTATAATTTTAATATTATAATTAAATAATATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAGTCAATAACAAGACCAACAATAGATAGAGCGAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGAGGCGCAAAATTCGCGGTATGTGTTGATAAAAATGCCGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGACTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTACTAGTTGAATGTTTAAACAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGAGAAGACTTCAATTATAGCTATTGCGGTTTTGAAATAACTAAAAGCAAAAAATATGGTAAAATTTATTTCTACTATATTAGCAATATAGATGATATGGATTAATGAATATTAAACATTTTAAAATCTTATCAACTTAAGATTAGGAGTTTATATATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTATCAGTATCAATGACAACGAGATCACCACGAGAAGGAGAAATTGATGGTGTACACTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGTGCTAAACAAATTTTAGAATATTATAAAAAACGTAACAGATTGGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGCTATTCTACAATATTTCATCATGTGATAGTTAATGATGATCTAAATGTAGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAATTAATGGTAGACTTTTGCATAGCGTCAGATCAAGGAAAGTTTAGAAAAGAAAACCAAGATAGAGCCATTGTTGTTAATGGAGAGTATTGAACATTGGCTGCTTTGTGTGATGGTATGGGCGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAACAATATTTCCTAGACACATTTCCTCAAAAGTTGGAATGTAGTGATAAAAAAAACGTTAGCAAATGATTTAACAATGCAATAAGCTTTATAAAAGAAGCTTTAGTTAATTATGCAAAAGAGTATACTGAATTTGAAGATATGGGGACTACAATGGTTGTTGCCCTCATTTTTAATGCAAATGGTTTAGCTTATGTCTTTAATATTGGTGATTCACGCTTGTATGCATACAATGGATTACTTTATCAAATCACAGAAGATCAAAATTATTTATATCAGTTAATGAGAGAATTTAATTTAACATACGAAGAAGCAGCATTAGATCCTAATTCATACAAACTTATAAGTTGTCTAGGTCCCAATAAAAAAACCAATTGTCAGTCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAAGAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTTGAATTAATTGGGACTGGAGGATTTGGCGAAGTTTATTCTGCTACATATAAAAAAAATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGTTGGACAGAGCAGAGAACGAGATTCATTAACGAAATAAACATATTAAAGGAAATTCATAGTTCTAACGTAGTAAAAATATTGGGCTGACATTATAAGGGAAACGAAGTTTTTTATGTAATGGAGTTAATTAAAGGTCAGGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATCGATCCTGATACTGCAATTATGTATGCAAAGCAAATATGTCAAGGTTTGATTGAAATCCATGCTGCTAAAATAGTTCATAGAGATTTGAAACCAAGCAATATATTAATTGATGAAGAAACAAATAGCCTAAAATTAATTGATTTTGGTATTTCAATAGGTGAATCAACTCAACGTGTAACTAGAAATTATAAAGCTGTTGGTTCTGTTCAATATATGGCACCTGAATTATTGCGCGGAATAGCTGATCCTTCTCCAAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGAAAGGTTCCTTTTAATAACCATGATGATATTAACCAAATTATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCACAAGCATTAGAAAATATTGTAATTCGTTGCACCGCAAAATCTGCAGAGCAAAGATATACTGATTGCATCGAACTAATGCATGATTTAGAGACATGTTTAGATTCAAAAAGAGTAAATGAAAAAAGATTAGAATTAAATCCAAGTAAATCAAAAATGAAAGCCAAAAGAATCTTTAATAGTAAATTATTCAATATTATTTTTTTCACAACAGGAGGCTTTTTAGTAGCCTTTATTGTGATATTCGTGGTTTTATTTTTAAAGGGGATATTGTAGATGATAGAAGGTAAAATAGTTAAATCGGTTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGAGGAAAACTTCGCTTGCTTGATATAGTGCCAATTGTGGGTGACAACGTTTTGTTTAAGAAAGACGAATTTATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCTTTGGTTGAACCTGAATTCAGTTCATTGCTCGTTGACAAATTTTTGATTATTATTGAAAGTAAGAATGTTAAACCTATTATCGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATTGCTTTTTATAAAGATCAAGGTTATGAAGTTTTTGAAATTAATTACGAAACAGGGCAAGGATTTGAAGGCTTAGGAGAAATATTTAGACATAAGACAAGCTTTTTTGTTGGTCAAACAGGTGTAGGAAAAACTACCTTGATAAATTATTTGGCAAAAACTAATTTTGAAACACAAGCAATTTCTAAAGCCTTAAACCGTGGAAAGCATACAACAAGAGAAGTTAATTTGATTGATTTTAATGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCCATAGAATTTGATTTAACAATTGAAGAACTACCAATTGCTTTTAAATCGTTTAGAGAAGCTGCAAGCAAATGTAAATTTAGAACAAGTTGTAAACATTATTTAGAATCCGTTGAGGATTGCGAAGTTAAAAAATTAGTTTTACAAGGCAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATCAAATAGGAGAAACTATGAAAAAAATAAGTCCATCAGTTTTAGATGTTGAAAAGAATAAATTAACAAACTATATTGACCAATTAGTTGATTGAGGTGTTTGCAATGTTCATTATGATGTAATGGATGGAATTTTTGTAAACAATACTGCTTTAACATTTCAAGAAATCAAGCAAGTTAAAGAAAATTGTAAGAAACATACAATGGATATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCATTTGAAAATAGTGATCTTGAAAAGATGATTATGCAATGCCAAAAAGATAACGTTAAACTTGGCTTGGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGCTTTGGTGGCCAAAAATTTATTGAAAATTCATTTAATAAAATAAAGGAATTAGCAGACATTCGTAAGGCTTTAGATTTAAACTTTATTATTGAAGTGGATGGTGGAGTTAAAGACCAAAATATTGCCAAATGTTTTGAATGTGGCGTTAATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATTGACCAACTTTTAAAATAATTATAATGAAAAAAGCAAATAAAATATTACTGTCTACATTTGCAACTGCAATTTTAGCATTGCCTTTTGCCACAATTTCTTGTAAAGTCGATCAAATTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCAGCTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTAAAAACCCGTTTAGTAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTCTTGAAATTCAACTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAAGGAATTATTAAAATATTATAAATTAATGGCCTTGCTTCAAGATAAGTCTGAAGTAAATAAATACGCAGATTTAATAAAAAAATATTCAAAAAAATTTTCTGATGTTTTGAAGGATGATAGTTCAATATTAAATGATATTTACAATCAAGTTGCAAATTTAATTCCTAGAATTAATTTACTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATTCACTTTTTTGAATAATAATGGAATACTATTCAATTTGGATTTTATATTGAATAACATTGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAAAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTTTATAAAAATTATGAACTAGTTGAAGATTTTAATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGCAAATAATTATTTGGTTAATTGATTAACAAACGAACCAAATATATGTCAAATCGGTGAAGATAAATTTTTAATTTATTACCATTTACCTTTTTCAATAGAAAATAAAAAAATTGTTCAAAAGCCTTCATTGTATATTTCAAATGTTGTTGCTGATTCAACAATTATTAAAGCAATAGCAAGCAGGAATGTATATTTATTAAAAAACTTATTTTTGTTGCTGCCAAAAGAAAATTACAATGAAAATTCAAAAGTCTTAAATAACTTAATGAAATAAGCATTTGAAAATTAGACTAACAATTAAAATACTGGTCTAATTTAGCTTTGTTAAAAGTTTATGCAAAGTTTGAATTGAAAGTATTGACATGTACATTGTTAAAAATATCAAATTAAACAAGATAATTATTAAAAAACGCTTATCTTGTTTAATTTGATTTTTAATTTTGTGTATTGAAATAAAAAATCCTAAGCAAAGGCAATGTCAAAATATAGTAAACAAAGTAATATAATTAAAATGTATAAAAAAAGTGCTAAAATTTAAATAAATTTTTGAATAAATTAAAATAATAGATTAATAAAGAATAATTATTTTTTGCTTGTGCCTACACTTTTTAACTAACTTTTTAAATTGATTGTTAAGGTTCATAATTCTGAGCAAATTAGAAAGGAAAAAAATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCATATTATGTGGAGGTTTAGCGGTGGCATCAATTGCTACAGTTGCTGCATACGCATGTAAATCAAGATCAGAATCAAAAATAGAAAAAGAAAATCTTTTGAATGAACTTAAAGAGGCATTAAATCAATTAAAACAATCGATTGCATCACCAGAAGCTAAAAATATTGAAAAATCAATGGAAATCAATATCTTAAATAATGCTAATGTTTCAAAAGAATCCTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAACAAGGAATTTGAAAAATTTGAACAAATCAAAAACGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATCGAAATTAAATAACATATCGCAAAGCTCAAAAGAACAAGAAATTATAGACGCAATAAGCAAATTGTATGATTTAAAGAAATGAATTTTAGCCCAAAAATTATTAATAGATAATCCAATAAATGAACAAAAAAGACAACTTAAGTCCTTAATTAAAATTGCTAATCAAATATTGTTATCAAACAAAATAGTTGAACGTGACCTAGAATTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAACAAAACTGCATCTGAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCTATTATTTGAAGAAACAATGTTTAAAGAAAAGGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACAATTTTTGAACATATTGGAAAAGACCCAAATAATAGCATATTCGATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACACTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGTAATGAAATAAATAATCTAGACAAATCATTTTCTAAGTTGGTTTCTGATACCCTTGAAGAATTTGAATATGAGGATGATCTTAAAGGCCATATTGATGTTGCAAAAGATCCCAAAAATTGAATTAATATTCAAAAATATATCTGATACATTAGGACAATAAGCCAAGTTAAAAAACTAAACTTAAAATCGTTAGTAGATTTTTATAGCAATGATCAATTTGCTAAATTTATTTGCAATACAATTTCAGACTTTTTCGATTATACAAGAGCAAAGAAAGATTTTGATTTTACAAATTCTGTTATTGCAAATTATGTACTTAATAAAATTATTAAGATCAGGGATTTTGAAGAATTTAAAAACTTAAATAGTTATTTTGCAGATAAAAAAAATGATATTTGAGCAATGCAAGTAGACCAAATAACAGACGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTTTGAAAGCAAACTATTAAGTGCAATATTGTTGCCTACAATATTTAAAAAGCCATATGAAAATCTTTCTAAAATAGTAAAGCCAATTATAGATATGATACATAATAAAGACGAATATAGCATAGAGATTATTGGATGAGAAAATGCTTCACAAGAATTAAATCTGGATATATTTAATATAAAAGAAGCAGAAAAAAAGGCTTTAGAAATAAAAGCAAAATTGACACAAGAAAATTTAAAAAATAAATTAAAAGAATTTAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAAGCATCCGAAAAAGGCATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAGTTCTATTATTAGAATAAAAAAAGAAATAAGATATCTTGAATATAAACTAGATTAATAATCACTAAAATATAATAAAAAAAATCCTTTTGATAGCATTAAACCTGGACAAAAAAATTAGACTCGTAATGAAAAAATACGGGTCTAATTTTTTTGTCCTAGTTTAGTAAAAAGCGTAATTTTAATCTTAATAAAAAAATTTGAATTGAAAAGAAGTTCAAAAGTTAATTTAAAAATTGCAAAATTTTGTGTAAATTTTAATTTTTTTATTTTTTAGATATTAAAAAGCAAAAATTAAACCAATTTTTGCTATATGTTTATTATTTTCTAAGAATTTTTTGACCTTTAAAGGTAAATCTTAAATTTATATTTCCTTTACAATATTTATATCAATACCAATATCTTTTCAATTTCAAGGCGTATTGTTCTTATTTCCTTTATAGTAAACTTCTTTTAAAGAAGGAATCAAGCTACTGATGACGTTATTGTTTCAAACTCCAGCTAAAAACGCATTAGTTCCAATTTCTTTAACTGAATTTGGAATAGTTACTACTTCTAGTCTTGGGTTTCTAAAAGCATAGCTACCAATTCTTTCAATTCCTTCATTTAAAACTACTTCTTTTAGATTTTTGAAACCCTCAAATGCGCCTTCTTCGATTTCTTTAACAAAATTTGGAATAATTACTTTTTTACTATTTTCAAAGGCTTTTGCTATGCCACGAGAAATGCATGTTATATTGCTATCTAAGATTATTTTGCAATCTGAATTAAACACATCTCGACCAAAACCAACAATATTTTTTGGCAAAACAACCGATGAAATACTAGTTCCTAAAAAAGCATAATTGCCAATAGATTTTAATCCTTCATTTAATTTAACTTCTTTAAGTTCTTTAAAATCCCTAAATGCACCTTCTTCAATTTCTTTAACTGAATTTGGAATAGTTATTGATTTTTGATTTTGAAAAACCCCTGTTATATTTCTATTAATTTTTTCAAAGCCATATAAAGCAATCTTACAGTCTTTATTAAATATATCTCAAGATAGTCGAGTTACTGAACTAGGAATAGTAACAGAAGAAATTTTAGTGCCTAAGAATGCTTCGCTTCCAATACTATTAAGTCCATCATTTAAAGTTAATTCTTTTAAATTATCGCAATAATAAAATGCATTAGCATAAACATCTTTTAAACTATTAGGAAAAATAATAGTAGTAACTCCCGCGCCTTCAAAAGCGTTTTCACCAATTTTTTCAATTCCATTGCTAAAATTAATCTTCTTTAAATATCAACAATCCTTAAAGGTTTTTGCCTTAATTTCTTTAATTGAACTAGGAATAGAAATTGAAGAGATTTGTGTGCCTTCAAATGCACCTTCTCCAATATTTTCAATACCATTTTTTAAAGTTAATTCCTTTAGATTTTTAAAGTTTTTAAAGGCTTCATTTTCTATTGATTTTACATTTTTGGGAATAGTAACAGAGGCAATTTTTTTAAGTCCCTTTGTTAAATCTTTAGTAATTTTGTCAAAACCATTATCAAATTTTATTTTGCAATTTTCAAAAGAAGCATTTTCATCTATTTCCTCTACCTTTTTGCCTTTAAAATAGCTAGGTATAACAACATCGATTTGTTTGCCATTATAATTGGTAATTTTTCAATTATTTGAAGAATTTCTTTCAAAATCAAAAAGTTCATATAATTCTTTTTCTTTATTATCTTGAACATTTTGATTTTCGTTATTTGCAGAAGAATCCTCATTCTTTAACGATTTTGGCTTTTTGTCATTAGGTTTGTTGTCATTATTCTTTGTAGGATTATTATCTATATTTTGATTGTTTGAATTGCTGTCTGTTTTTTTAGTACATGATGCCGCTACTAATGGAAAGCAAATTGTTGAAGTTGTAACAAGCCCTAAAGTTAATAACATTTTTATTCATTTTTTCATATTTAATTCTCCTTATTTTCTTCTTTATCTTCAATAAAATTTGATTTTGAATCATATTATTACTTTATTTTAAATTATAACTATTTTTAGTGGCTTTCTAGTGCTATTTACGTTTAAAAATATTATTAATTTTTATTAGTCATAAAAAATATAAACAATTTTTTGCTTTATTTTTTATATCTAAAAACATACTTTATTAAATAAAGATATATGGAAATAAAAAAATAAAAAATTTTTAGCACTCTTATGCTTTTAGTGCTAAAATATTAAATGTAAATTGAAAAAATTCAAATTAAAAAATATATTTTAAAAGGAGATTAATTATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTAGCTTTTAAAAATGGCGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTTTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATTACAGTTCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAAAACGCAGGAATTATTGCTGGTCTTGATGTTGTAAGAATTATTAACGAACCTACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCGTTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTAGCAACAGCAGGCGATAACCACTTGGGTGGTGATGACTGAGACCATGAAATCGTTAAATGAATGGTTGAACAAATTAAATCTAAATACAATTTCGATCCAACAACTGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACTCGTGCTACATTTGAATCAATGACTGAGCATTTACTACAAAGAACAAGAAAACCATTATTAGATGTTTTAAGTGAAGCAAAATTAACATGAAATGATATTAATGAAGTGTTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAACTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCAGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGATGTTACTCCTTTAACATTAGGTATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATCGTTATCACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTCAATCTTGAAGGAATTGAACCTGCACCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGATAAAAAGACAAACAAAGAACAAACAATAACCATTCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGACGCAGAAGCAAATAGAGAAGCTGATCAAAAGAAACGCCACGAAATTGAAGTTATTGTAAAAGCAGAACAATTAAGCAATGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAATCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGAAATAGATGAAATTAAAGAATTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAACGACAACCCTCAAACTAACTAAAAATAATAAATTTAAAATAACGGTTTTAAGCCGTTATTTTTTTGTTTTTGTCTTTAAAAAAAATTCTTCAAAATAAAAAGCAAAAATGGGACTAAATATTGCATTTTTCCCAAAATAACACTTTTTTTATAACTTGATTAATATTTTTTTTGTAAAATTTTTAACATAATTGAAAATTTTAATAGAAGTTAAAGTTTAAAATAAATGGAGGAAAATAAAAATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTAATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATAATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATCTGAGGGAAAATTCTAAGTTTTAATTCCCTTGGACTTATTTGAACATTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGGCATTGGTTGCACATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATCATAATTTTTGCACCTATGGCAATAGGTGGCGGATTTATATTTGCCAACTCAACATCAACAAATGAAAATTTTGCTCATTATATTAATAGCAAATATAAATACCATAGAGCAGGAAATGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCCTGAAAATATTCAAATAGTTCAGCTAATGAATGGCAAAAATATTCATGACTTGCAATGCCATATCAATTTGTTGATATATTCAACATTGAAAATCAAAATATTTTTAGTAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAACTAAGATTAATAAATACTATGTCCCAGGGATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCTTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGAAAAATAATGAGCCAAGTTTTAAAATATCATAAATTTACAGAAGCAGCCGAAGAATTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCACATGATATTAAAAAGTTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTGCAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCGTTGCTATATTTCATCTATTTTAAAGATAATCAAGGTCAAGTTATTAAATCATTATTGAAATCTGAAAGCGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTGCAAGAAAATAAAATAACTAAAAATAAACAAGTTGTAGTTAGATATGAATTAATTCCAAGTAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGAGTAGTAGTGGCAATAATCTTAATGATGGCAAATAGATTTCTATATCTAAGAAAGGATTATAAATAATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGCGAAAATGGTGCAGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGCGGAATTACAAACTTAAACCCTAATTCTAAAAAAATTCTTGGTTATGTTCCAGAAAATGCAGTGTTTCCTAAAGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCGGGGATAAATAAAACCGAAGTAAAGGCAAAAATTGAAAGTTATTTACAAAGATTTGGAATTATTGAATTAAAAAACGAAAAACCCGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAAGCTTTAATTAACGAGCCTAAATTATTGATTTTAGATGAACCAGCTGCTAATTTGGACCCTACTGCAAGATTTGAATTGTTTTCAATATTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCGTTGACATTAATTCATGGTGGAAAGATCTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCGTTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATTGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATCCAAGCAAAAATTAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGACATTTTTGTACAAAACCAAAATAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTTCCTATTTATAATAGAGCCTCAGATAATCCAGGTAGCGAACTTTATGGATTTATAAGGCAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGATATTGATATTTAAAACAAATTGGTAATTTTGTATATTCAATAAACCCTTATAATGATAGATACATCGAAACCCCTCAAACAAAAAAAACAATTCAATCTGCCCAAAAAGACATTGGTATTTTATATAAAATAAAAGATGCAATGCCTAAGGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTTGCTAAAAAAATTAATGAAAGTATTGATTCTAAAGTTCAAAACCAATTTAGCGATTATTTAGAAGATAGAATTGATGAATCATCGAAGTTAAAAATAGATACAAATTCAGATATAACCAATTTTTTAAAAAGCAAAATTAACTTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAATAATAAGAACAAATTTGAAATTAGTAATCAATATCAAATTAATGATGAAATTTCTAATAAAATATTTAATCTTTTAAATACAAAAGGATCTTCATTTGACAACTATGCTTCAAAGAAAATCTTCTACGTAGCATACGACCACAAAACTTGAATAAGAGTAATTGTTTATACTGAAAATAACGTTAAAAAAATTTCTATAAATCCTGATGTTTATATTTTAAATCAAAGCAATAGCTATGAACAAAGCAAAGAAATTTTAAAAAAATTAAATAAACAAATTTTTATTGAACGGTTGAATTCAATTATTGATTCTTTAATTGAAAATATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACTGCTATATCGTGCGTTGATAAAAGCGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGGGACGTTGTAAATAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAACGACTTATTAAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTGGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGAAATTCTAAATTAAGCAATATAGAAAAAGAAAATATTGCCAATCAATTAAATCAAATTTATGAAAAAAATTGATATATTGTATTAACAAATATAAATAAATTTAATTTAAGATTCTATGAATGACTTTTGTTTGATAAACCTGAATTTTTAAGTCAAATTAGAGCTGATGGAAAACATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAGAAACCAGATAATGTTCCAATTATCAATAATTATTTAAAGGAATTAAAAGAAGGTGATGAATCAGGGGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTAACAAAACCAAAAATTAAACTAACGTGTTTTGTGTGATATTTTAGTAAATTTAAAAATATATCAATAAATTTGATTTCAGATATTGTCCATAGTGCTTATATTCACGGAGTTCAAGAAGGATTTAATAGATTTGAAAACGATATGGCAAATCGTTATGGAAAACCCGCCAAAATGATCATGGGAATTTTCGATGATAATGCTTCTAGTGCCAATGGAGGAAAAGAAAATGAATAAAAAAATTTTGATGACTCTATCATTGAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATTGAGGGCAACGAAAATGCAAACGAAAGAGAATGAAAAAATTTTTTAAATAGAGATTATGTTAAAGAAATTTTAAGACTTTCGTTTGACGATGATGATGATGCTAAAAACAAATATATAGCCGAACAAAAAAAATTAAATAATAATATAGAATATTCAAAAAAATTAAAAGAAGCATTGATATATGGAAATTCATTGAAGGCTTCTTCTAGTTACAATAGCGATGATTTTTTAACACAACCTAAATTATTGTTTCAAAATGGAAAAAAGATTTTTAATGAACTTAAAAGTAAGAATTGACTTTGATTTCTTTACAATCTTCCAAAAATAATTTTTGGTTATTTTCCTCCAATGAATAAATTTGAATCATCAGATGAACAATCAGCATTAACTGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAGGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGAGAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTCTTCAATTAACTATTGATTGAGCCAAAGACGACGAAGATAATAAAATAAAGCCTAGTGTTAAACTTTTTGGTTATATTTACACCTATCCAAACATAGTAAAAGACAGAAATAATTTAAATATTTTCAATATTCAAAAATATGTTATTGCCAATACCGAATCATTTGATGATATTAACAATAGAACATTGCAAATATTATTTCAAGATCAATATGGGGGTAGCCCCTTACGTTATACAATAATTGACGTTAGTTTAAAATAAAATGTTAATAAGATAAAAATTCAAGTGTACACGAATTGAAACAAATTAAGTGTTGACTTGATTTTTTTAAATTAAAATTTCAAATGATATGAGCAATAAATCAATATAAGCATCTTTTTTTGTTTTTAAAATAAAGTTTTTTAGCATATTGTGAATTTTAAAATTTAAACAATTATTTTTTTATATTTTTTGATACAATTTTATTGTATTTATTACAATCGGTAAATTATTCAATTATATTAATTTATCAAACACTATTACGGATTCATTTAACTAGTGTTAGTAAAGATTACTAATGTTAAATGTTAGAAATAATAGGAAGAACAACAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGAAATCCAAAAATGAAACAATATATTTGAGGAAAAAGAATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGAGTAGGAACAAAAAAACAAGCTAAAAAGGCTATAGAATCAGCCGCAGCAAGAACAAATTCAGTTTATGTTTCAGAACGTTGATTAGGTGGAACATTAACTAATTCACAAACAATTTTTAGAAGTGTTAGAGAATTAGAAAGATTAGAAGAACTTCAAAAATCAGAATATCAAGGATATACTAAAAAAGAAGGTCTTTTAATGGACAAGAAAATTGCTAAATTGCAAAAGAACTTAGGTGGTATTAGAAAATTAGCTGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCAGCAAACGATGATTCTGTAAAGTCAATTACTTTAATTACAACTATCTTGGCAGACGCAATAGCATCAGCACATGGTGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACTGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATCAGAAAATACTAAGAAATCTGAATAAAATAATTGAAAGGGAATGTTATGTCAGTAGATTTAAAAAAAATTAAAGAATTAAGAGAAAGAACAAATTCAGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAATAACGATATTGAAAAAGCAATTGAATGATTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCCGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTGATTTTTGAATTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGAAGATTTATTAGAACAAAAAATTAACGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGTGAAAAGATTCATTTAAGAAGAGCATTAAAGTACGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAACAGAATTGCTACTATTTTAATCGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCCCTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAAGAAATTTATGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAGCAGAAAATAAATTACAATTAATAGATGCAACAAGATTTGAAGTTGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAATAATTAAGTGTTAAAAAAATAGGCTTTTTGCCTATTTTTTTTGATACTTTTTAGTTATTTTTTTTAGTTTAATTAAGAAATGATTCAATGCAGAACGAGACTTTTTAATGTTTTTTGTTTCTAATAATAAACTAAGTTCATTCAAGGAACTGTCTAAACTATTTAATTTTAACTCAAATAGTGTAAGTTCTTCAACGCTAAACTCATCCAAGAGATTGTTTTCTTTTATAAATTGATAATTTTTAATAAATTCTAAATTTGCTTTTGCAATACGTTCTTGATTATATACGTCGAGATTTGTTATTCTATTTGCCGAATTATAAAAATCTCTTTGAATTTTGCCATCTTCGAATAAATAATAAGATTCAATGGTTTCAATAGCTTTTAAAAAATCACAAATATTTTCAATTTTTTTTAAATAAATTATTATTTTTTGTTTTCTAATTATTTTTTTAAATTCTAATTCGTGAGAATTTAAAATTGATAAAGCATTTTCTGCATTTATTAAATCATTAAATTTTAACTCTAAATGATTTGTTCTTCCATTTATTATTGAACCCGAAGAAAGGAATAAACCAGCAAAGAATTTTCCTTGATATTTAACACTTACGTTAGTTAAGAAATTTGTATTTATTGAAATTTCATTTTTTTTAGTATATGTATAAGAAATTTTAAGTTCATTGCAAAGATTTAATATATATGTCCTAATCTTAATATTATTTATTACTAATTTAGCTTCATTGTTTTTTACACTGCAACAATAAATTATGCCAGCCATCAAAAAAAACTTTTCTTGTTTTTTTAACTGAAAAGACATAATTTCTTCTTTTACTAGTTGACTAAAAGATTTATTCTCCATAGATGATTTCTTTCCTTTAATATATTAAATTATATTATATAGGAGTCTTTATTTAATTTTTATGCCTGCATTAGCCATTAAATTTAAAGCAAAATCGTGATATTCTTGAGAATTATGATTTGAAATATTAAATGTTGCAATTAAATTTTTAAAGACAATAATTTCTTTATTTTGCTTTAAATGATTGAAATAAGTTTTTAGAGTTATTGCAAATTGTAAAATGCAAATATCTGAACAACAACCAATAATTTCAAAGGAATCATACTTGCTTAAAATTTCCTTTTTTAATGCAAAAAAGGAGTTAGTAGAATTCTTTTCAATTATATTTTTAGCATATTTTTTTAATTCATCTACTACTTGGCTTTCTTTACTATTTTTTAAACAATGTATTGGGTATGATTGCATTTCAATATCATCGCTACTATGAGCATCATTTAAAAAATATATATCATTGTCTTTATATTGAGTTTCTAAAAAATTTTTTATAGGAACAATTATTGACTCAATATTGTTACTATAAAGTGCGCCTTCTTTGCAAAAACCATTAACCATATCAACAACAAAAATTGCTTTCATATTGTTTACTCCTTCTTTTTCATTTTTCAATGTTTTTGTATTGTCATATAAATTGTTAATTATTGTATAATTATAACGGATACATCTTAGAGGGACATTTATTTTTAATTATATGTTGGTGTATCTAAAGTTCGAATTTTATTTATTTTAATATATAAGAAAGGATTTCTAATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGAGCATTGATCGTTTGATTAATTGCTGGATTTGCCGTAATTACCATGGGTATTGCATTAGTTGAAGTTGCATCAGGACGTAATGACAACTTAGGTGTTATAGGTTGAACCAAGGCATTTAGTACATTAAGTCTATACAAAGGTACTAAGTTCTTTATGACCTATCTATATCTACCATTTACTTACTTCTTTATGCCTTACTACGTTATCGTTCAATTCCAAGACGGTATCGCAGGATTCGTAGGATACAGCAAAGTAGGATTTGGTGGTTCTACAAGTGCTCCATGAATCTACTTTGCAATAGGTCTAGCACTTACATTATGAATGATTTTTTCAGCCGGTATTAGTGGCCGTGCTACAAACATTCAAAACTGAATAGTTACAGTTGCAAAATTAATTCCTATTTTCGTAGTTATTATTTTGGGTATTATTTTTGCTGTATACAAAGGTACAGGAAAAGAAGGATTCGTTGCTGGAGTTGATGATGGTGTTACACCACTATGACACAAAATAACATCAGCTGACCTATTCAACAAGAGTTCATCATCATTCTTAGGATTAACACCATTCCTAACTGTATTTAGCTCACTAGGTGGTATCTTCTTTGCATTCGACGGTTTCTATGTAACCGCTGGTATTCAATCAGAAATGAAACACCCAGAAAAAACCCCTGCTGCTATTGTTATTGGTTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAACAACGGTTCATTCTACGGATTTGGAGATGAATTAAACGCTGTTAAAGCAGGTTGAGTATTTGGTGTAATTAACATTTTAATCTCAATCGGTATTATTGGTATTTTAAACGGTTTTACCGCTTGAGCTACAAGATGAGTAGAAGACTTAGTTAGAGAAGGTGAAATGTTCATGCCTGTAAAAGCTTACAGATACATTAAACATTCACAAGTTCCATGAGTTGGAGCAATCTTCGTATTATTAATTTCTCTACCATTTATGGTTATCTTCACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGAATGGCTGTATTCGCATTCGCATTCATTGCAACATCAATTATCGGAATGTTACAAAACAGAAAGAAACACTTTATTGCTGTTGTTGAAGGAAAACACAGTAAATGAACAGGATACGTATCAGTTATCTTCATCTTATCAGTTATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCTCAATACGTTGCAGATAAAACACAAGACAACAGAAACGGCTTAGTAGGAAACATTGCTACATCAGTTCTATTTATAATATATGCAGCAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAACATTTGTGCACACGAAGTTCCAGAATGATGCCCATGCAAATTAGAAGAATTAAAAGAAAAAGCTGAACTAAATGACATGGTTCTAAAAACATTTGCTGAAGCAAGATAATAATTTGCTATTTCACCAGTGGGTTACTCCCACTGGTTTTTATTTATATTTTTAATATTCAATCAAAAACTTAATTTTAATGTTAAAATGTAAAGATAATTATTTTATAAAGGTAACCAATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAGATGAAACATTGATTAACACAAATTTAAAAACCGGCCTAAGCTCACAAGAGGCCACCTTAAGACTTAACAAGTTTGGAAAAAATGAGTTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAGCAATTTAAAGATATATTGGTCATTATATTATTATTTGCGGCAATAATATCTTATGCTCTTGCAATTGTTTCAGGAACTCAAAATAATTGAAATTGATCTATTAATGGTAATCGCTTATTAATCGAATTTATTGAACCTACAATTATATTATTTGTAGTTCTTACTAATTCGCTAGTAGGTGCTATTCAAGAAATTAAGAGCATACAGGCGGTTGAGGCATTGAGCAAATTAAGCCCCTTACAAGCTAAAGTCGTCCGTGATGGTAATTTAATAACATTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGTTATTTGTTGTTTAGTTCAAATTTAACTGTTATCGAGAGTGCATTAACAGGCGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAGAGAAATTTAACATTAGCAATGGCAGATAGACAATTTATGGTTTATTCTTCAACTGTAGTTGCTACAGGAACTGCTTATTTTGTTGTTACATCAGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAATTAGTTAATCAACAAAAAGAATCAATGACTCCTCTACAAATTAAAATTAATAAATTAGGAAAAATATTTGGATATTCAGGTCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCGGTATCAAGAAATTCTTTTGCTGATACAATGTTTTGAGGCAATGCAATAGTTGGAGCCATTTCACTAGCAGTTGCTGCCATTCCTGAGGGATTAATTGCCTTTACTTCAATTATTTTAGCAATTGGTGTTCAAAGAATGGCAAGAAAGAATGCGATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGCAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAACAAGATGACAATTGTAGATGCTTTTGATGGAAGTCAACTACTATCAAATAATAGTGAAATTAAAAACTTTTTAAATATTATAGAATTAGGTTCGTTATGTTGCGATGCAAACTTGATTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTTTCTAATTTTAAATATAAAAACAACCTCGAAATAGAATATAAGCGTTTGCATTTGTTCCCGTTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACGACAACAATTATTGAGCAACGGTTATGGGCGCTCCTGAAATAATAATTGAAAAATGTAATTTTGATTCACCTTCATTAAAAGAAAAATATTTGCAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTTTTAGCAATAGCAAGAAAATCTTTAAAAAAGGAAACAATTGAGAAATTCAATGAATCATCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATCGATCCTCCAAGAGAAAGTGCAAAAGATGCTATTACATTATGCAAACAAGCAGGAATAAAACCTGTTATGATTACTGGTGATAATTTAAATACAGCAAAAGCAATAGCAACAAATCTTGGTATATATAGTGATCAAGATCGCGCTATAACAGGAACTGAACTTTCAAACATTTCTGATGAAGAATTAGTTCAAAATATTGAAAAATACAGTGTTTATGCCAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCTTGACAACAAAGGAAACAAGTTGTAGCAATGACTGGCGATGGTGTTAATGATGCTCCGGCATTGAAAGCTTCAGATATAGGCTGCGCTATGGGTATAACAGGAACCGAAGCTTCTAAACAAGCTGCAAATGTTATATTGGCAGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAATGGAAGATCAATCTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCAATTGCAGAGATCATTTTATTATTTATAGGTTTATTTTTATTTAAAGCTATTTTTTCAAGTGCTGAATGAAAAGTAGCATTAGGAAAAAGCGATTTATATATAATGTCAGCTACTCAATTGTTGTGAATCAATTTGTTTACTCATGGTTTTCCGGCAATTGCATTAGGGCTACAAGATTCAAAAGAAGACTACATGAATAGAAGGCCTATATCGAAATATGAGTCAATCTTTGCAAATCAAATGGGATTCAACACTTTGTGAAAAGGAATTTTGATTGGAATACTATCGTTAATAACTTATTATTTAGCAGCGTTATATGGCAGAAATAATGGATTTAATGCAGAAGATATATTAAAAGCAGCATCTACTTGTTCATTTTTGGTTTTAGGTATATCTGCTACATTCAATTCTATAAACTTAATGACAAAGAAATCAATCTTTATATCTAATCCATTATATTATTGAAAAGTTTATGCCTCAGTAATTTTTTCATTAGCATTTCTATTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGCTACACTGAAATTCATTCTAAGCCCGAACTTTTAGCACTAGGATTAGCATTGCCATTAGTAATTATTCCAATATATTCAATTCATAAATTAATCATATATTTAATTGAAAAAAACAAAATTAAAGAAAATAAAATTATTGAATTTAAAATGATATTACCTCCAAGGCAAGTCAAAAGAAATCAAAAAAGACATTAAAAAAATGTAGATCTATAGAAATCTACATTTTTTATATTGCAATATAAATTATACAATTTCTTTTTGCTTCAATAGCTTCATTAAAGGCTTACGCTCATTACAGTAACGAATATAACAAAGGCAAAAAACATAGTTAAGCAAGAAATAATAATTTCTGCTATTGACAAAGTTCTTCCTGAATCATTCATTGATTTCGACTTGCTAAGTCCAATACAGCTAAAAATAAGACCTAATATCGAAAAGAAAAATGACAATATAAATCCAACAATAGCAAATTCATTAGTTGCTGAATTTTGGATTTGTTGTTGAGGTTGCAAATAAGGTTGAGATGGTTGCATTGTATTCATTTGTGTTGTTACATATGCATCTTTTTCCACTTTTTTTCCACAATTTGGACAATAATTAGCATTATTTAATTTAGTGCCACAATTTGGACAATAATTTATACTACCCCCTCTTATTAAAGTACATAAAAATTATTTTTTTTAAATTAATTCTATATAAATATAACACAATAAAGCAACAAAAAACATCCCAATTCTTGTAGGGATGTTTTTTATTTAATTAATTATTTTTTTTGTTTATATATATTTTATTTTCTATATCAAAAGTTTCACCGTCAAGTTGCATAAAGTGCATTTCCTTACATTCCATGCTAAATTTATATCCTTGAATTTCATGTACATATTTTTTATAACGTAAATGTTTTCCACTATATACTGAAAAGAATAATCATGCTAATTTTCATTTAGAGACGTTATTAATAATGATTAAATTTAATTTGTCTGTATTGTTACAATTAGGAGCTAATTTCATTCCACCCCCAAAATAACAATTATTCATAATTGAAGCTAATCAAACATTTTTAAATTCTAATTCTTTTCCATCTACATTAATTTTTATTAAAGGAATTGTTTTGAATGTCTTAAATGCATTTATTGTTACTTTAGCAAATGACGATTTATTTTTAAGCATTTTTTCATCATTAACTTTTTTTGCAATAACTGCATCAATACCAAAACCAATGCCATTTAAAAAAGATCTTTCATCTTGGTCGTTTTTAAATTTAATTGTTGGTAAGTTGGTAATGTAAGGAGATATCAAATAATATTTTTTATGTATTATTTGAACATCTTGTTTTATTTTTAAAGACCTTAGAAAATCATTGCCAGTTCCTGCTTTATATGCATAAATTTTGGGTAAAGGTTTTATATTGTGAATTTTATTAGTGATATTGGTTAATGTTCCATCACCGCCAATTAATAAAACAATATCTTTTTCTATATCCATTTGAGAAACTAGATTTTGACTGTCTTTAATTTTTATAATGTCAAATATTTTTACCTCAGGTTGTAAAAATACTTTAATGGCGTCAGAAACTATGTCATACATCTTTCTTTGACATTTTCCACTCTTTGATAGAGAATTATAAAATATATATAGCATAAATTATTTTTCCAAATCTTGTAATCTATTTAATGCATTGATATAAATTTCAAGTGATGATTTTATTTCATCAAATGTAAAATATTCGTTTGGTCCATGCATTAAATGCATATATTTAGTTGATCCAAAAGCGACACAAGTTTTTATTAATCTTGCAAATGTACCGCCACCAATTGCTAAAGGTTTTTCATTTGAATTCATCGCTTCATTATATGTATCCATAAGAATTCTTACAATTGGAGAATCTTGTTCAATATACTTGGGTTCTTTGAATCCTTGCAATTTTGAAAATACATCATCGCCAAATTCATCTTTTAAATAATCATTTAAGCTTTTAGTTACATCATCAATTTTATGAGAAACTGGGACTCTAAAATCAAAAGTTAAAACATATTCATTTTTCAAGGTTCTAATAATGCCTAAATTTGCAGATAATTCGCCCGAAAAATCACTATAGTTTTTAAAAATATTACTTAAATTAAATGATTTGCTATTTAAATTTTCTTTAATAAATTTAAATAATTTAGATTTAGCAAATTCTTTATTTTCTTTACTTGCTATATTAATGGCTTTAATTATTGCATTGTCACCTTTTTCAGGTGTTGAGCCGTGTCCACCAATTCCCTTAATAATATTAGGTTTTTCATTCAATACTAAAGATGTTGAATCAGGAATTTGGTTAACAACGTCGCCCCCATTTAATTCAACTCCTGGAACCTTTGGAAAATGAATTTCTGTTACATAGATCATTTTTTCAGCATATATTAAAGGTCATTCACCATCAGGAGTATAAGAAATATAAGGATCTTTGAAGTCGGCCAAATAAGACTTCATTGACTTCATTGTGGTTTCTTCTGAAATTCCAAATATTATTCTTATTTTTCATTCGTTAGATAATAAACCATGATCATGAATGTATTTCATTGCATATAGATTAATAATAGCAGGTCCCTTATCATCTAAACTACCGCGTCCAATAATTGATTCATTTGTTATAACTGGGACAAATGCGCTTGTACGTCATTGGCTTTCATCTCCTGCAGGAACAACGTCAAGGTGGGCTAATATTCCAATAATCTTATCTCCATTGCCAAATTCAGCAAACCCATAACGATTTTTAGGATCTTTATAAATATTAAATCCAAAACTTTTGGCAAGATTTAATGCATAATTTAAAGCATTGTCAGTTTCTTTTCCAAAAGGAAATTCACTGCTAAAATCTTCTTCACTAATCGAAGGAATTGCACAAATATTTGCAATATTTTTAATCATTTCATCAAAGTCTTCTTTACTTTGCTTATAAGTAATAAATTTTTTCATTCTTACCTCTTATTAATTTTTTTAATATATTATACATTTTAATCCAAATAAAAAAAATAAATAAGGCGTAGCCTTATTATTTGTTTAATCCAATAGCAATACCTTTGTTTTTAAAAACATAGTCATTATTATTGTTATATACAACATCAATTAATTCAGGATAATCAATAAAAGGATTTCTATTTCCTTGATGTTTATATACTCCATTGTTTCTATCAATGTCAAATTTTGAAACCTTATCTAAATTATTTCAAGCTAAAAATGTTTTTAAAAATATAGGAGTTATAGTATTTTTTCTATTAATTTCTTTAAATACACGAGTTGATTGATCACTTGATGTTATATTTAAATCGTTATAAGTTAAACAAAAGTATAAATATGCCCTAGCAACATCACCTTTAAATTCATTAATAGGTTCAGTAACAGGTCCACCATCTTCAACTGACAAACCAGTTTTTGTACCATTTTTGGATATTTTATTACCTTGCAATTCCTTAACTGTTCCATAAGGGTAATTTGCATGCCATGCATTAACTAATTTATCGGTTGGTCAAACATGGTGAGCATCAGTTCTCATTGGAGCAGACTTACTAAATCATGATTGTGGAACTAAGTGTTCTCTATTCATTCCATCGCCTTCATGTTTGCCTTGATCTCTATATTTTCCATGTCAATTAGTAAAAACATCTTCTTTATCTGGAATTTCTTCATAGATATCTAAAACAGATCCATCTTTTTCGTAATATTTATCAACAAAAGCATCTTTATATGTATCAAATAAGTCTTTATATTTTGTTGAGTGATTTCTATGAGCTTTTTGCAATGCAAATAAGTTATCCCTTAGGTTTTGTCCGCTTAAGCCATCTAAGGATTTATAAAAGTCATTTGAATTATCGTAAATTATCTTATTATCTAAGGGTTTAGCTTCAGGCTTCTTTTCAGGGGTTGTTCCGTGTTTTTGTGATGTCGCCAAATTTATAAAACTATTTAATCTGAATGTAATTTCTTTGATAGCATTCAAATCATCACCTTTAATTGCTTCTAATCCTTTCACATATTCTTTATTAAAATTAAATTTTTGGTTAAAATTAAATGATTTTGTATTTATTTTTGCCGCATGTTTTAAAGTTGAATTTAATTGCTTCTTTGCTTCGTTTAATGCTGCTTGGTTTGTTCTTTCTTCAATTGATTTTTTAAATTGATCTAATAATGATTTAGTTTTGTTTAATTCATCATCTTTAATTTCTTTATGTTTATTAATGATGTTTTCTATTGTGGTTTCACCTTTTGGTTTAACTTCAAATTTCTTTGTTTTTACACATCCAGCAGCAACTAATGGCGCTGAAATTAGCAAAGGTAAAATAGCACCCGTAGTTAGGAATATTTTTTTGGTTTTTTTCATTATTTTATATATTTCGCTTTCCTTTTTTGTATTTAATAAAAATTTATCATATGAATTAAATAAGTCAATTCAAATTAATAATAATTTTCAAAAAAACAAATAAAAAAACACCTATAAGTGTTTTTTAATGTTTAACAAAGAGTTTGCAAAGAAAATCTTATTCAGCTTGTTGTTTAGCAAGTCTACGGCGAGCTCTGGCTTTTTCTAAAGCTAATTTATGTTCTCTTTTAGCTGCTTTTACCTTGTTAGCTAATCTAACGTCTTTTCTCATAATTTCTCCTTTGCTTTGTTCAAGCAATAATTATATCTATTTTTTCTTAATTTGTTTAATTTTTTGCATTATTGTTTCAAGATCTATTTTTTCAATTTCTTGTATTTTCTGACTAATGCTTTCTTTTGTTGCTATATTTTTTTTATCTTTTTGGTTGTCATTAACATTATTTGAAACATTTGGTAATTCAATATCTTCAGTAGATATTTGAAATAGTTCTTCTTGTTCTTCATTATTTAAATGTTGAATATCTTGATTATAGGATTTTTTAATCAAAGCATGTTCAGCGGTCAATTCAATATTTTTATCAATATATCCTTGATATTCTTGTCTTATTTTTTCCTGCTCTATTTCATCAGCTTCAATTAATTCATTAATTTTTATTGGCGTAATAAAATACCCAGGGTTAGTAAATGAATAATCTAATTTAAGAAGTTTACTTTCCTTAGTCTTATCTTTTAATATAAATTTTCAATCATATTCTGCCAAATTACCATTATTATCAGTGTATAACATTTTTAAATTGCTCAATGTAACAGCAAGTGATTGAAGACCTAAAATATTATTTTTTAGTGGCGTAAACAACATTTTTGGAGTATTGCGTGTGGTCAATTCTAATTTGCTAGTATCCCAACATTTTATATAACCACCTTGTGTTAAATAAATTATATTGTTGTATTTTGAAACTGTTATAAAACCACTTACTTGAAGTTGAGGATGTTTATATAAGTTTAAAAGAAGTCTAATGCCTCTTGCTTTCAAAGAAATTCCAACTTTTAGTTCGTTTTCTGAAACTAAATGATACATACCATTATTTAGTAAAATAAATAGCATTTTTTCATCATTAGAAATTTGAGCATCTATTAATTCATCATTATCATCTTTAAACGACATGCACGTTTTTGTTTTAAGAGAAGATTTATTTTCAAAATCTCTTAAATTAACTTTTTTGGCATATCCTAATTTTGACATTAAAATAAAGTTTGCATAACTATTGAATGACGTAATTTCTATAACTCTAATAATTTTTTCTGAGCTTTCTAAAGCTACTATTGATGAAATATGTTGGCCAAGATCTTTTCAATTGCAATCTTTAAACAAATGGCAATCAATAATAAAATAATTTCCTAAATTTGTAAAGAATAATATCTTTGATAACGAGTTTACTTTATCAAAATAGAAAACATTATCTTCTTCTTTTAATTTGAATGTGTTTAATTCATTGCTAGTATAAACTTTTAAACTTATTTTTTTATAATATCCATCTTTGGTTATATATAAATAAAAATCTTCATTTTTTGTCAATAATTTATGATCAATTTCTTTATCGATTTTTTGATCTGAAATTTCCGTTAATCTTGGCTTTGAATATTGATTTTTTATTTCAAGCAATTGTTTTATTAAATATTGATTAAATTCTCATTTATCATTTAATAATTTATTGCAATTTTCAATTTGAATTTCAAGGTTTTTCTTTTCTTCTTGAAATTCAATTTGATCCATTCTTGAAAGCTTGTACAATCTTAATTCAGCAATCGCTGTTGCTTGCAATTCGCTAAATTTAAATTTATTCATCAATGCAAGGATAACACCTTTTTTTGAGTTATCGCTTTCTTTAATCAAATGTATAACTTCATCAGAAATTTCGGCTACTTTAATAAACCCTTCAACTATTTCTAGTCTCAATTTAAACTTCTTTAAATCATAATTAATACCATTTATATTAACTTCTTTTAAATGACTTAAATAAGCAATTAAAGCACTATAGAGATTCAAAATTACTGGTCGGTTATTGTCAATTGCAACCATGTTATAACTATACGAGATACTTAGTTCGGTTTTATTCATTAAGTATGCAATTATTGCGTCAGCATTTGCACCGTCTTCTAATTGAATAAATATTGAAATTCCGTTTCTATCTGTTTGGTCCAAAACTTCTTTAATACCAGAAATTTTTTTATCTATTGCAATTGCATCAATGTCGGCAACTAATTTTGATTTAACAACCCCAAAAGGAATTTCAATTATTTCAATACCAATAATTTTCGATTCATCTTTTTTATCATAAACATAATGATATTTCGAAGAAATTGTAATTCTACCTTGCCCACTTGAAAGAGCTTTTTTTATTTCATTTATACCATTAATAATTGCTCCTGTTGGGAAATCTGGGCCTTTAACTATTTCACTTAATTCTTCAATTGATATTGTAGGATTTTTGATTAATGCAATTGTTGCATCAATAACTTCGCCTAGATTATGTGGTGGGATTTCTGTAGCAAAACCAGCGGCAATTCCTTTAGCACCGTTAACTAATAAATTAGGAAATAAGGCCGGCAAAACAATTGGTTCGTATTCAGAGTCATCAAAGTTTGGAACCATTTTTACAACTTTTCTGTCTAAATCTTTCAGCATCAGTTCACTAATTTTTTCAAGTCTTGATTCAGTGTAACGCATTGCAGCAGCAGGGTCATCATCAATTGAACCTTTATTACCATGCATTTCAATTAATGGGAAATTACTTTTTCAATCTTGAGCCATTCTGACTAATGCTTCGTATATTGAACTATCTCCATGTGGGTGATAACGTCCGATAACATCCCCAACGATTCTAGCTGATTTTTTAAAAGGTTCGCTATTTTTTAAATGTAAATTTCACATTGAATATAAAATCCGACGTTGAACAGGTTTTAGTCCATCACGAGCATCAGGAATTGCTCTTTGTTGAATAATGTATTTTGAATAACGCCCGAATCTATCAGACATTATATCGGCCATATTTTTTTCAATAATGTTTTCAGCAACTTCTTGTATTTCTTCTTTTCTATCTTTTTTCATACGACCTACTTATTATTTATTTTAAAATCATCTTCTGATGAAAAATCAACATTATTGTTTATTCATTGTTTTCTTGCTATTACGTCGTCACTCATCAATGTTGAAACATTTCTTTCGACTAAAATTTCATCTTCTACATCAACTTTAATTATTGAACGAGTTTCGGGGTTCATTGTTGTTTCTCAAAGCTGATCAGCATTCATCTCGCCAAGCCCTTTGTAGCGTTGAATTTCATAGTTGGTATATTTTGAAGTAATTTCTTTTAATTGATCTTCATCCCAAGCATAAAGGGGCTGTGTATTTTTTGATTTTATTGAAATCTTATAAAGAGGTGGCAAAGCAATATAAACCATACCGTTTTCAACTAGTTTTTTCATATAACGTCAAAAGAATGTTAATAGTAATGTTTGAATGTGAGCACCATCAGTGTCAGCATCTGTCATAATAATAATTTTGTGATATTGAGCATTTTTTAAATTGAAGTCTTCGCCTATACCCGCTCCGATTGTATTAATTATTGTTGCAATTTCTTCATTTGCTAAAACATCGCTTAATTTTGCTTTATCGGTGTTGATAACTTTACCTCTAAGTGGCAATATTGCCTGGGTAACTCTATTTCTTCCTAATTTAGCAGAACCGCCAGCTGAATCACCTTCAACTAAAAACAATTCTTTAATGGCAGGATTTTTACTTTGAGCAGGAGTTAATTTTCCTGAAAGAATCTTTCTATTCTCAAATTTATTTTTAATCTTACGTGCTTCGTTTCTTGCGTTTCTAGCTGCAACTTTTGCTTCGTATGCTTTTTTAATTTTAGAAATAATTTTTTCTGCTTCAATTTTGTTTTCATTTAAAAAGAAATAAAACTTTTGACTTACAAAATCTTCAACAACTTCTCTAGCCTCTGGCGTACCTAATTTATCTTTCGTTTGACCTACAAATTCTAATATTTCTTCTGGGACTTTTAAAGAAATAACAGCAATTATTCCTTCTCTAACATCCGATCCTTCAAAAGTTATTTTTTTAGCTAAACCATTTTTAGAAGCATAATCATTTATTGTCTTAGTTAATGCGGTTTTAAGTGCTGTTTCATGTGTTCCACCATCACGAGTCTTTACATTATTTACAAAACTGTAAATTGATTCACTATATCCATCAGTATATACTAATGCCAAGTCAATTTCAATGCCGTAATTGCACATACCAGATGCATAAAAAATTTCACTAATAAATTTTTTGCCTTCACCAACAAAATTTATATATTCTTTTATGCCATCTTTGGTTTTAAATGTTACATGTTCATCGGTATTTTCATTATAAAATTCAATAGTAAGATTTGGCATTAAAAAACTTGCTTCTCTTAATTTTTCTTTAATTATTTCAGGTGAATATTTTGATTTTTTAAATATTGAAAAGTCGGCTCAAAATTGAACCTTTGTTCCTTGTTTTGTAGTTGGAGATATCTCGTGCGTTTTTTCAATAATTGTATCTTGCTGAAAGGCTGTAAAGTATGCTTTTTTATCTCTTCAAACCGTAACTTCAACTTTAGAACTTAAGGCATTGACAACTGAGGAACCCACTCCGTGTAATCCCCCACTTGTTTTATAAGCGCCATCGCCAAATTTGCCCCCAGCATGAAGCTCAGTAAAGACGAGCTCAACTCCACTTTTTCCAGACTCGTGTTTATTGACGGGGATTCCCCTACCGTTGTCTTCAACAATAACAGAAGAATCCTTCTTTAAAGTTACAATTATTTTATTTGCAAACCCACTCAACGCTTCATCAATAGCATTATCAAAAATTTCCCAAATTAAGTGATGAAGCCCACTTGAATCAGTAGACCCAATATACATTCCCGGTCTTTTTCTGACAGCATCTAATCCTTTTAAAACTTTTAAATCGCTAGCTTCGTAATTATTTAAACTCATATCTAAATTATAAAGAAAAAAAATCTCTTGAACTATTTAAAGTAATAAGAGACTTATTTTAGAAAAATTTTGCTTGCGATTTTTATAATTTGTGGATTTGAAAGATTAAAGTTTCTAATGTCTTTAAAGTCAAAAAAAGCAATGTACAAATCAAAACTATCGTCATCATTAATTGCAAAAATTAATTTAGACCCAATTGGATATTTTTCTAATTGTTTAAGATCTGGCATTTTGACGTTTAACATTGCCAAGGTCGTAGCAATGTTGGTATCGTGGCCTACAATCAATGTTAAATCTTTGCCGTTGTTGAATTCTTTTTTAATTAATTTATATATATTTTTTTTATTTGAATTTATAACATTTTTGTCGCAAAAAGCAAAGTCAATTACATAATCCTTAGCCTTACCTATTAATCTTAAATCTCTTATAAAATTATTAGATTTAAATATTGAATCATACGGTTCGTTGAAGTAGTATTTAACTACTAATAAATCACTTAAAGAACAAGCCATAAAGAACTTGCCTCTTCAAAAAACACCATCTTTATTTTCATTCAATGTAATATTGGTTTCTTCATTTGTAAATAAACAATTTTTTAGGTCAAAAAGGTCAATAATTTTTTGATATATTTTTTTACTTTTTGCTTCATATCTTCATAACAAATCATAATCAACGAGATTAATATCGTCAGTGTTAATGTCATATCATTCATTCTCATTTTTGAATGAATAGTCTTGACATTGAATGTCGATTTTTTTAGCAGGAAAAAGACCAATTGAAAGCAGTTGTGCAGTTTGAAAAGTTCTCTGCGTTGAATTTGCCATCACCTTAACATCAATATCATTTGTTATATTGAAAAATTCTCTAATTTTTTGGGCCATTTTTAGCTCAAAAAGTGCTCCTTTATCTGTTAAAAATGCACTTCCTAAATTATCAATGTTTCAATTTACAATATCTTTATTAAAATATTTAATCGAACGTTCTTTTGTAAAAAATGGATATCTTAAACCATGTCTACTTAAAATTAATAGTTTCACAATGTCACCTCATTTTTTCAATAATTACGAGTAATTATTTTTTACACAATTGCATAATCTTATATAATTATATTGTTATATAAATAATTTTTAATTATATAAATTTTAAGGAGAAAAGATGAAAAAAACTAGAATTGCAATCAACGGTTTTGGTAGAATTGGTCGTCTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGATTTAACAGATGCTGCTACATTAGCTCACCTATTGAAATACGATACAGCTCATGGAAAATTGAATGAAAATATTTCACACACAGAAAATTCTATTATTGTAGATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGAAAAGAACTAAACGTAGATATAGTTATTGAAGGAACTGGAAGATATGTAACAAAAGAAGGTGCTGAATTACATATTAAAGCAGGCGCTAAAAAGGTATTTATTACTGCTCCAGCTAAAAGCGAAGGCGTTAAAACAGTTGTTTATTCAGTAAATGAAGATATAATTACACCAGAAGATAAGATTTTATCAGGTGCTTCATGTACTACTAACTGTTTAGCTCCTATTGCCAACGTATTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTCCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACAGGTGCTGCAATAGCTATTGGAAAAGTTATCCCTTCATTGGATAAGAAACTAAATGGACTATCTTTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCCACTGTTCAAGAAATTAATGAAGCAATTAAAAAATCTGCTTCTGAATCATTAGAATATTCTACTGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGCGTGTTAGAAGTAGATGGCAAAAAACTATACAAAGTTTATGCATGATACGACAATGAATCTTCATTTGTTGCTCAATATGTAAGAACATTAAGACATATTGGAAAATTAACAAAATAGTTAAAAAAATATTGGATAAGAAATGAAACTTTCAATAAAGGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTCGAAGATTTAAAAAAAGAAGAATCCAAGGAAAACTTCATTGTTTATTTAGAAAACAAAAGGCCAACTAACATTGAATATGATTATCAAGATTATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAATTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATACAAAAAATATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAAAAAATTTCTAGGGTTACTACAAACGATGGTCAAAACGAATATACGGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAAATTAAACGAAGAAATTTCTGAAATTCAAATTGCAATTGATAGTGCAAACATTAATACATTAGTTGAAGTTAAGTAATTAACTTTCCTTAATAAATTTGCTTTATTTTCTATAATTTTTTGTTTATTGTTATAGATTTCTAAATTATTAACCTTCCGTTTTAAGTTTGTGTTTATATGATATCGAATTTACATACTTAAATTAGCTAATTTATTTAAAAACTTCTACATTTAGTAAGGGTTAGAAAATGTAAACCACCATTTGAGGTGGTTTTTATTTATATAAATTTATAAATATTAATAATTAATAATATAATTAGATAATTATGAATACAACAAAAGTATATATTGATTTTGAAGCAATTACCAACCCTTTTGCAAGATTAGTAAATTTGCCAAGTGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGCCAATATCAAATTAAAACATTTATTATTGATTTTAAAGAACATCATAATTTAAATTCAATTTGAACTGCTATAAAAAATGCAATAGTTTCTGACATATTAGAGATCAACAATACTGTGAATTTAAAGGAAGTTGTATTTGTAGGTCACAATCCTGTTTTAGAAAATAAATGCTTAAAAAAATTATTTCCTGAAAATAATGTTGAACCTTTAATTTCCAATACAATTGTGTCATTGTCAAAATTAACAGGGAAAATTTTTAACATTCCATATTTTAATAAAATAAAAAAAATTATTGCTGATTCTGGTGATAGCGAACTTAATATGAGAATTAAAGATAGAAATGGTGTAATTGCTGCATTTGCTGGATATTGATTATATACAAAATCAATTAAAAATTTACGAGCAAATGACAGAAGAAAAAGATTTTTAATTAAGATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCATGACGATGTCAATAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTTTACTAAAAGAACTTTTATACAAACGTGAACTAATAAAACAAATAAAAAATTTGGAACTTGACGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGAACATTATAATTTCTAAAAATAAAGGATATTATGAAAAATAAGCTTATTGAAAAAAAAGGTAAGTTTTCAATTAAAAAATTTTTCCCTCAAAGCAAGTTTGATTGAAAAATTTATATTATAAAAACATTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGACAATTTCATGGTTAGCCATATTGATGGTGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTTTCTATTCAAGCAATCGGGGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGATTTTGATAAAGTAAAGCAGATAATGAATTTAAGAATTTGAATGAATCTAATTATTACCTTAAGTTTTGCAATTCCTGCTTGAACATTAGCAAAGCAAATGGTTCACTTGATTGGTGGGCAAGGAATAAAACCTCAATCTGAATTAGCATCTACATATTATTTAATGTTTATTGCCATAAACTGAGTTTTAACTTCTTTTAATTTTAATACAAATATGTTGCTAAATGAAACCGGACATTCAAAATACGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATACGGATTTCATAAACCTGCATATTATGCTGCTTTTGGTTCAATTATTGCTGCGGCAGTTTGCTTTACAAGTGATTCTTTATTTACATATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAATTGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCTGGAATGTTAACTTTACCTACAAGAATGATTATTTGAAGCCATGCATATCCTGATAATTTATCAGGTGGTTCAGGAATTGGTGAAAAATGAATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCGTCCGTCGCCTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCGTTTTTTGTTGCCACAAGATTAGGAGAGTCTGATTTTGAAGGTGCAGATAAGCATGCTCATTCATTGCGGGGTTTTCATGCTTTGGCTGGATGTTGCATGTCTATATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTACTACAACAAAAGGTGTTGGTATTGGTGTCTCTCAAAGATTTTATGATGTGCAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAATTCACTTGGCAATGATTTTACATTCACAAAAGGTCAAAATTTGGCTTATGACCAATGGGTGGCAAGCGCAGTACTGGCAACTTCAAAAGAATTCAAAGAAACATTTTTGTTATGTAGTGCAACCTTTATTTTCTTTAATCCTATATGATGCTGATTTTATACAAGCGCTGCTTTACCTGCTGCGGGCGGTAGAAATATAATTGGTTCTATTACAATATTAGCAACACAATGAGCATCATTTATATGATTAATTATTATTACTTATGGAATTGTTATTCCATTGCATAAAACTTATACTGGATATAGTGATTTATCAATTCCTCTAGAACTTGCTTATTTTATGTTATTTGCATTTGACTTTATTAGATGATTGATGTTTGAAATAGTAGCATTAAAGACTGATTGAAAGAGAAATATTACAAATGAAATTGAAAAACAAGATCCAAACTTTCCAAGTAATATAGCAGAAGCAACAGCAACACACGATATTGTTCATAAACAATAAAAAGGAGATTTAAGATGTTATATGATTTAGTTTCACCATATAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGCAAGCCAATTTTGTTTGTCCATGGTGGTCCAGGTGGGGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGTGGACTTAGCATACCTAGTGCCGAAATCAAACAAAATACGACCCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGAATATTGTTCGGGGGGTCATGAGGTTCAACTTTAAGTTTACTATATGCAATTAATTATCCTCAAAACGTTTCAGGAATAATTTTGCGGGGAATATTTTTAGGTAGGGATGAAGACAATTATTGATTGTATCAAAAAGGCGCTTCTTATTATTTTCCTGCTGAATATGAAGAATATTCAAACTTTATTCCTGAAAATGAAAGAGGCGATTTAATTAACGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGAAGCAAATGGGAACTTGGATTGGTAACAATGAAAAAATTGCCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGCAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAAATATTTTTAAATGACGATAATTTTATTTTAAATAATGCAAGCAAGATTAAAGAAATCCCAACAATATTGATTCATGGTCAATTTGATATGGATTGTCGCCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAGTCAAAGCTTTGAATAATTCCAGAATCAGGCCATTCAGGTCGTGATGGACATATTAACGAAGCATTAATGCAGGCAACTGAATACTTTAAAACAATTAAATAAGCGTTTGCTTATTTTATTTTACTAAATGATTATGAAACAATAAATGCAATATTTCTTATAATTTAAACAGACATTTAAGGAGAGAAATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCAGAAGGGGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTGTATGATGTTGAATTAAATAAAAGAAAAGAAGCCATATTGAATATATTGGCCGAAAATAAATTATTAAATGATGAACTAAAACAAAAAATCGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAAGCATCAGATGCTATTGCTCTTGGTTTAAGTCCGTTGGCCGATGAAATTTTAAATAATAATGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGAGATTATGGAGTTATTGAAACTAAATTAAAGAAGAATGCAATTGATGAAAATGAAACTTTCAAACAATATTATAATTTCTCTGAAAGAGTTAATAAAATTCCAAATCATAGAATTTTAGCAATATCAAGAGGGGAAGATAAAAAAATTCTTTCATATGATATTACTTATAATGAAAAGAAAATTATTTATGATCTAAATCAAAAGTACTTCAAATCAAAACGAACTGTTAAATGTATTCAAGAATGTATTTTAGATGCATTAGACAGACTAATTATTCCTTCAATAATTAGAGAAATTAAAACCGAATTATTTGAAAGAGCTGAGGCAGAAGCTATTAAAGTATTTGCTTCAAATGTTGAACAAATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCATAATCAAGAACAATGATTTGAGGCAATTAATACAATTAATAAATTTATTGATGAATACAAAATAGATTTAATTGTTATTGGTAATGGAACAGCAAGTAGAGAAACTGAATCGTTTATTGATAAATTATTGCAAAAAAGACTTGAAAAAAATCCTCATGAAAAAATCAAATTTGTAATTGTAAGTGAAGTTGGCGCTTCTGTTTATTCAGCTTCAAAAATTGCTCAAGAAGAATTTCCACAATTACATGTTGAATATAGATCAGCAATTCATATTGGTAGAAAGTTTCAAGATCCCTTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAAGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTTGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAATACCATAGCAAGCAATATTGTAAACTATAGAGAAGAAAACGGTTCATTTAAATCACGCGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACTATTGTCAATAAGCTAAATATAGATTTGAACAATATTGACAAAGAAAAATTATTATCCGTTGATAAAGAACAACTTGCAAAAGAATTAAACTCTGATAAATACAGCATTCAACTAATTATAGAATCATTGATTGCACCTGAAAAAGATATTAGAGATGATAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATCGATGATATAACTCAAGGTCAAATAATTAAGGGTCAAATTCAAAATGTTACCGATTTTGGCGCCTTTGCGTTTATAGGATTAAAACAAAACGTTTTGATCCATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAATATACAAATAATTTCCATTGATAAAGAACACAATAAAATTCAAGGAAAAATTATTTGAGATTAATTAATAATCAATATTGCAACATTGCTATTGAAAAAAATAGCAATGTTTTTTGTTGTAATTTTGTAAAATTTATATATAAAAGTTATTTATTGCACTGGGGGGAATTATGGCTATTAATAATCAAGAACGTGATGAATTACATAAAAAAATTTGAGATATAGCAAACAAATTGCGTGGTTCAATTGATGGTTGAGATTTTAAACAATATGTTTTAGGAATTATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAGCTTCAAGCAGGAATTGAAGACTTTGATTATGCAACTTTATCAGATGAAGAAGCGCTGACAGGAAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAAAAACGCTCCAACAAACAATTGTTTAAATGAAACATTGGACAATATCTTTAAAAATATTGAATCATCAGCAAAGGGCCAACAAAGTGAAAATGATTTTTCAGGTTTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTACCAAGATAATTCAATAGATGCCTTTGGAGATGCTTATGAATATTTAATGTCAATGTATGCATCGAATGCTGGAAAATCAGGGGGTGAATATTTTACTCCTCAAGAAGTTTCAGAATTGCTTACTAAAATAGCGGTTTTCAATAAGAAAAAAGTCAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTACTCCTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGTCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATTGGATTTGATAAATTCAACATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATCGTTTCAAATCCTCCTTATTCAATTAAATGAGAAGGTGAAGACAATCCATTGTTAATAAATGACCAAAGATTTTCACCAGCTGGAATATTAGCTCCAAAAAGCAAAGCCGATTTTGCTTTCATACTTCATAGCCTTTCATGGCTTGCAACTGATGGAGTAGCTGCAATAGTTTGTTTTCCGGGCATTATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAATTTGTTTTTCGGAACAAGTATTTCAACATGCATAATGGTATTAAAAAAATCAAAAATTGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAATAAATTAACTAGTCAAAATATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCAAAATTAGCTAGTGTTGAAGACGTTAAATCTAATTCATATAATTTATCAGTCAATAGTTACGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGCGAAATTGACAAAATAGTTGCCGAATTAGAGGAATAGTTATGAATAAAATAGAAAAGTTAATTAGGGAACTATGTCCTAATGGAGTTGAATATAAAAAATTATGTGACTCTTCAATAATATCAGTTGGTAGTAGAATAACTAAATCAATGATGAATGAAAGTGAAAAATATCCTGTTTATGGTGGTGGAACAATTCCAACAGGATATTATAATGATTTTAATAATGAACATTCAATAGCTATTTCAAGAGCAGGAAGTGCAGGATCAGTAAAATGAGTGAGCCAAAAATATTGGGCTACTGATGTATGTTTTGTTGTGTCTGAAAAATATGAAGTTGCAAATATTAAATTTTTATATCATTTTTTAAAATTAAGAGAAAATGAATTAAAAAAACACATTTATGGAGGAAATTTACCTAAATTAGATAAACAATACTTATGAAATTTGCAAATTCCGCTCCCCCCACTTGAAATTCAAAATCAAATTGTTAATATTTTAGACAAGTTCGCCGAGTTGACCGCCGAGTTGACCGCCGAGTTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAGAATTATTAAATAAGATAATGAATAAACAACAATGTGGTAATAGTATAGTTGAATATAAAAAATTAGAAGAAGTAGTTGACTATTTACAACCTACTAAATGAATTGTTGAAAATACACAATATAGTAAAAAATACACCACTCCTGTTCTAACACCAGGGAAAAGTTTCATATTAGGCTATACAAATGAAACAAATAATATTTTTGATGCGTCTGAGAGCAATCCAATCATTATATTTGACGATTTCACTTGCAATTTTCATTGAGTAAATTTCAAATTTAAGATTAAATCTTCTGCTTTAAAAATTTTAATTTCCAAGACTTCGAATAATAATTTTAAATATATTTTTTATTATATGAAATTTTTAAATTTTTCATCAGATGATCATACAAGGATGTGAATTAGCAAATATTCTCAGTTTAATATTCCACTTCCTCCTCTTGAAATCCAAAATAAAATTGTTGAAATTCTTGACAAACTTGAAACATATACAAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTGCTGCTTTTAAATGAACTTTGAGGCAAAATTGTCAATATAGTTGAATATGATTTAATTAAAAACTTGTGTGAAATAAAGCGAGGAAAAGTTTATTCAAAGGAATTCATAAAATTAAATAAAGGTGAATACCCTGTTTATTCATCTCAAAGTTTAGACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAATATGTAACATGAACTACAGATGGAGCATATGCCGGGACAGTATTTTATAGAATTGGTAGATTTTCAATAACTAATGTGTGTGGAATATTGAGTGTTCTAAATAAATCAAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGAAGACAAAAAAATTTGTAAACAAGGCAAGCGGAAATCCTAAACTCATGAGTAATATTATGGAAAATATTGAAATCCCCATCCCTCATATTTCAATTCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATACACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGAGACAAAATTGTCAATATAGTTGAATATAAAGAATTAGGAACCATAATCACAAAGGCTCCCAAATCTCCATTTGGAGTTGCAAAACTCTTAAAAATGAACAAAGGGGATTATTTAGCAATAACTTCAGGAAATAAATCATTTTATGTCTCTGATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGTGGGCAAGCAGACGTTAAATATAATCAAGGAAAAACAATGTATTCCGATCATGTGTTTGCGTTTAAAACAAATGAATGCAACACAAAATATCTTTACTTTTATTTATTGAATATTTCTAATTTTATTAACGAAAAATTATTTGTTGGTAGTGGATTAAAAAATTTAAATAAAAAAGAATTCTTGAATCTTAATATTGCAATTCCTCCTATTGAAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAAACTGGTCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAACATTACAGGGATAAATTATTGAACTTTAAAGAAAAGAATACTAAAATTTAATTAGTAGCAATATTAAATAAGGGTGTAAACTATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACAGTTGTTTCTGAATATAAGCCTCTAGCGAATAAAGCAAAAGATTATCAAACAGAGAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAACCAAGGATATGAGTATTTGAACATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGTTAAATAAAATAAATTTTTCAGATAATGAATGAAAAAAATTTTTTAATGAAAATATTGCAAATAAAAATTTTTCAATAAAAGATAAAAGTAGAATAATACAAGAGGATTATCGCCAACTTTTAACTAGAGATGATGGTAGTCATAAAAATATTATTTTAATTGATAAAGACAATATTCATAATAATAAACTTCAAGTTATAAATCAATATGAAGAAGAAACAGGCAACTATAAAAACAGATATGATGTTACTATATTGGTTAATGGCATTCCAATGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTCTAAAAGAGGCTTTTAATCAAATCATTAGATATCAAAGAGATTCGTTTTGGGCTAGCAACGGCTTATATGAATATGTACAAGTGTTTGTTATTTCAAACGGGGCAAATACCAAATATTATTCAAATACAACAAGATTACAACATGTTAATAAAAAATCAGATATGATAGATTCAAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGATCTGATTCAAAAAATAACCCTATTCATGATTTAATTGATTTCACAAGTACCTTTTTTTCAAAACATACGATTTTAAATATATTGACCAAATATTGTATATTTACATCTGAAGAAATGTTGCTAGTAATGAGACCTTATCAAATTGTTGCTGTTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCAGGTGGCTTTATTTGACATACTACAGGAAGTGGTAAGACTTTGACCTCTTTTAAAACTGCAAAATTAGCATCACAATTGCCTTATATAAATAAAGTAATTTTTGTTGTTGATAGAAGAGATTTAGACTATCAAACAATGAAGGAATATGATAAATTAGAAAAAGGCGCTGCAAATTCAAATCGTTCTACATTAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCTGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTGCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCAGAAGTGATTAAAACAACCGAACAAGCTTTCGGCCAAAAATTGCACACTTATACAATAATAAATGCAATAAAAGATAGAAACGTTTTGCCATTTAAAATTGAATATATTAAAACAATTGATGTTGATAAATCGCTAAACGATAAAGAGGATCAAAAAGTAAAAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTTGATCAAAAAACTAGGAGAAATTCCAATGTAATTTATAGCCATTCGATATTTGATAAATTAGATAAAACTATTAAAAGAAACGTGAGCGGTTTCAATTCTATTATGGCTGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAACCAACTTGCTCAAGATCCTAAAAACAATCTAAAAATTGCTTTAATATATTCATATGGGGTTAATACCGAGGAATCATATGGCGGTTTAATTGATGAAGAGGATAATGAAAATACATTGCAATTAAGTAAGCCAGATAGAGATTTTTTGGATGGTGCTATTAAAGATTATAATAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTAGACATGCTGATTGTAGTAAACATGTTTTTAACTGGTTTTGACGCCCCAACATTAAATACATTATGGGTTGATAAGAAACTAAGAGAACATGGTTTAATTCAAGCTTTTTCAAGAACAAATAGAATTCTAAATGATAAAAAGGTATATGGAAATATTGTATGTTTTAGAAATTTAGAAAAAGAAACTGACCAAGCAATTTCGATATTTGGCGATGGGGAAGCTAGTGGAATTGTTAAAATCAAAAATTTCGTTGACTATTATTATGGCTATAATGATAGTCAGCAAGTGCATCACAATGGCTATTGCGATTATATTCAAAAATTAAATAATGAATTTCCTTTAGATAATTGAAATGTAATAAGTGAACAAAAGAATAAAGAATTTATTATATTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGATGAATTTATTGGCAAGGAAATCTTAAACGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACTGAATTGTTGAAAACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTCCGTGGAAAGACAATTACTGAAATTAACAATTTAAGAAATGAAATATCTTTAGCTTCTTCTTCATCGCCTGAATTAAGAAGCAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACAAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAAAAGAAAGAGCTTGACAAAATTATTAGTGAAGAAAATTTAGATTTTGATAAGACCCATAAATTTATGGATGATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCATTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGGCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAAATTCTAATGTTTTTGGTTTATAATATAGTTACGTTTTATATTGATATATAAAGGGTAGGCTAAATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAAGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGATGAAGAAGCAGATCTTGAATTTATTTTCGATCAAGAAAATGACAATTTTGCAATTATTAATCATAATAAAGTTGTCGTTGGTGATCCAATAACAAGCGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCATTAAGTGATGCTAAAAAAATAGATCCAAATGTAGAAATTGGAGATTCTCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGACATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGCGAAGTTGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGACGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAATCAAAGAATAATTTCAAAATTAAATCCAGGCGATTGAATTGATGTTGTAATTGAAGAAGTAAAAGAAGAAAACAGCAATGCTCAAATAATTGTTTCGTCGGTTGAATCAAGATTACTTCAAAAATTATTTGAAATTGAAATTCCTGAAATTTCACAAGGCTTAATTTCAATAGTTAATATTGCAAGAATTCCTGGAGAAAGAGCAAAGGTTTCTATTAAAAAATCTAATGATGCTCCTGAAACAATGGAAGAAATTGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGAGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGAATACATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGCAATATCACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTAGCAATAAAAATGCTTCAAATAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTACGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAAAGCCTATACAAAGAAAGTCAATTGTTGATAATAAAATATACGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTCTAAACGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAAAATATAGATATTCAAAAATACAATGAAATAAGGAAAGAGGTGGACGAATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCATTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCAGAATTTTCTCTTAAAATAAATAAAAACCCTACTGAAATATTGACTTATTATTTTAAAAAAGGAATAATCAAGACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGACCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGGCATGTTGACCATGGCAAGACTACATTAATTGATAGAATAAGACATAGTAATGTTACTGAAACAGAATTTGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACCTTTTTAGATACACCCGGTCATGAGGCTTTTACCGAAATGCGTTCAAGGGGAGCTAAAATTACTGATATAGTAGTTATTGTAGTTGCGGCAGATGATGGAGTTAAACCTCAAACTATAGAAGCAATAGACCATGCAAAAGCAGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAGGGAGCCGATATTGAAAGAATAAAAAGCGAACTTGCTGATAACAATGTTTTATGTGAAGAATGAGGTGGAAACTACCAATTTGTTTATGGATCGGCTTTAAAAAATTCTAATATAAATGAACTACTTGAAGCAATATTTTTGCAAGCTGATTTACTTGATTTAAAGGCCAATATAAATAGAGATCCTATTGGGACAATTATTGAATCTAGAATTGACAAAGGACGTGGAGTTGTTTCAACTGTTATTGTTGAAAGTGGAACATTGCTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTCTAACCTCTATTGATGGCAAACCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATCACTGGTTTAAGCAAAAACCCTCAATCTGGTGATAAATTTGTAGCTATTAGCGACGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCATATTTAGATAAATGCAAAGAATTAAATGAAAAAAATGCATTTTCTATTCAAGATGGAATAAAAACTGTAAATGTCATTGTTAAAACCGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATAAGAAATGATGAAATTGCAGTTAATGTTATAAGAGCAACCTCCGGCGAAGTAACGAAAGCAGATATCTTGCTTGCTGAAACATCTAATGCAAGAATTTATTTATTCAACAATACAATAAATAACGAAATTAAAAAGGCGGCTGAATTAAAGAATATAAAAATCTTTAAACACAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTACGAAGAAAAATTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGTCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAAGTATACAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAGGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAGTCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATATTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTGGCAAAGAATTGAAAATATAAAGTTCTACCAGAATTAATTTTTGAAATTGATACTGTAGAAGAACACGCAGCTAAAATCGATAAAATTCTAAATAAGATTGAAGAAGAAAAAAATAATTAATTAGAAAGCAAAGGAAAAATATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGCGGTTCTGCAAAAGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGTTAAATACATCTAAAGGCCCAGCAGTTTGATCATTAAGAGCCCAAATCGATAAAGAAAAGTATTCGGAAATAATTTTAAAAGACATTGAAAAACAAAAAAATATTACATTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGTGTTGGAATTGAAACACTTGAACATGGTAAACTATATTCAAAAGTTGTTATTATGACAACTGGTGTTTATATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCCGATGGAGAAAAGACAAGCTCAACACTTTCAAAAAATTTAAAAAAATATGGTTTTGAAATAATAAGATTAAAAACTGGAACACCTTGTAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTGGAAAAAAATGAACTTTGTTTTTCAAGTCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTTGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAACCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCCGCTCAAGGATTAATAGCAGGAATTAACGCTGCTAATAAACTTGATAATTTGGAACCATTAATCATAAAAAGAAATGACGGTTATATTGGCGTTTTAATTGATGACATTGTAACTAAAGGCACAAATGAACCTTATAGAATGTTAACAAGTAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAGACAAAGAAGCACATGATAGGATTGAAGAAAAATATAAGATTATTGCTGAAACAGTAGAAAAATTAAAAAATGAATATGTTTCTTCTAAAAGTGATGTTGGTCAAAAGTACAATGTAACAAATGGTATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGTTCAAATTTTCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCGGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGAAGTGTTGATAATTTAGCAACAGAAGCAAGACAAAAATTAGATAAAATAAGACCTGAAACAATTGGCCAAGCAGCAAGAATAAGTGGAATAAATCCTGCTGATATTCAAATGCTAATATTTTATTTAAACAATAAGCGTTAGTAATGAAATTAAATATTGTAGCAGTAGGTTCTCTAAGCAAGGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTGATTGAAATAAAAGAACAAGTTAATAAAAATATTGATTTAAAAATAGCTGAAGAAACAAAATTAATCCTAGAAAAAATTCCAAAGAACTCTAAAGTTTATTATTTGTCATTAAGAGGCAAGAAACTTGATAGTGGCGAATTTTCGGATTTACTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTAACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAAAATAGGCTTTTATTTTAAAAAACAAGTCTATTTTTTTATTTCTTATTATTTTATTTAAAAGGAATAAAACATTTATAATTTAAATGTAACCATGTTAAATTTAAAAAATAAATTCTTGAAAGGAGTTTACATGGCAAAAAATACATGAGAAATTGCTAAACAGGCAATTGAAGAACACGATAATATTTTTATATTTCATCATATTAGACCTGACGGAGATTGTTTAGGCTCTCAATTTGGTTTGAGAGAATTAATAAAGGAAAATTATCCTGAAAAAAACGTTTTTGTTTTAGGGGATAACGGAGGAATCTTTCCATTTTTAGAATGAGATTTTGATAATTTTGAAAATATTGATAAAAAATATCTTAAGAATTCCTTAGGGGTAGTAGTTGATGCAAACTGCTCAAATAGAATTCAATTTGCTAATTATATTTTAGATGGTAATTTTACCCATATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTACGACTATACTTGGGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATATATCGCAATGATGGCAAAATGAAGAGTTACAACAAAAGCTGCTAAATACGTTTATTTAGGCATAAACACAGACTCTAATCGTTTTCAATATGACTATGTTCAAAAAAGAACATTTGATGTAGTAAGTTATCTTCATGAAGGCAATGATTTTAAAGTTTGAGACATTAACTTCCCTCTTTCAATTCGTGAAGAAAAGAAAGTTAGATTCAATGCCTATGTTCTTTTACATTACAAAAATGAAGGCAAAGTACTTTGATTCTATGCAAATAAGAAAATTCAAAAAAAATTTGGTTTGAATGAAAAAGAAGCAAATGATGTGGGAATTTTAGCAAATATTGGTGATGCTAAAATTTGAGTGCTATTCATTGATGAACCAAATGGCAAAATTAGAGCGAGAGTAAGAAGCAATGGAATATGAATAAATCATATTTGTGCAAAACCTGAATATTACCCTGGTGGTGGTCACGAAGTTGCTTCAGGAGCAACTTGTGCTAATAAAAAATCTATGAAACATTTAATAGAAGACTTAAAGGCAGAGGTATTAAAATATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAGAAAATTGAAGAAAGAATTAAAGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATTGGTGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTACCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCCAACTTTAAAGAACGTTTAGAATGTAGAGAATACTTGGATAATAATTGTTTTGATGAAGTAATAAGAATCGATCACCATCCAAATGATGATGATCTAGATGCTTCATTAAGATGAGTTGAACCAGAAGCACCAGCTGCTGCTCAACAAGTAACTGAAATAGCATACGAATTGGGTTGAAAATTAAACAAAGAAGCTGCAACTTATTTATATTTAGGTATATATACTGATTCAGTTAAATTGTCAACCAATACGACAACCGCAAAGACAATGTTGTTAGTAAGCTGATTGTGAGACAATGGTAGCCAAAAAGACTTAATTCATACAGAACTTTCTAAGCGTACATTGTTTGATATTAATATAAATACATATATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTAGGCTCAATTGACAATACAAGGGCATGAGTATTCTTTACAGAAGAAAAACCTGGCCAAATCCGTTGCGAATTTAGATCAAATGGTTGCAATGTAAGAAATGTAGCAATTAAATGAGGTGGGGGCGGACACATACGCGCATCAGGCGCTCAAATCTCTGATCCTAATTTAATTCCATTAATTATTAAAGACTTAGAACAAGAAACAAAAAATCTTGCTGATTATGAATAAAAAATAGAAATAATTTGTTAAAAACGGCGATTCTTATTTTATAGGATCGTCTTTTTTAAAAACTACTCTGAACGCAAACAAGGCTATGAAGATTTATCTAAACTAATTAAAGAGATGAAAAATGAATTAAATTCTTTAGATATGAAGGATATAAAGAATTTAGATTGAGTAAACGCAAAACAATCAGAATTAAATAGAAAACATAAACATTATTTAAAAGAATATAACCTTCTTAAAGAAGAAAAAAATTAAAAGCTAAAAACCAAGATTTGACAATACAACAAATCTTGGTTTTTAACTAGAATTAAATGCTAGCTATGAATAAAATTATCTAATATATAATTATCAACTTTCTTGTTTATTGATTTAATAAAAGAAAAATAAGTTTTGTATTTCTTAAAATCACCCTTTCAAACAAGCTTTTTATTTAATGAATTTGCTAAATCAATTGCTAAATAGACATCAAAAATTCCAAATTCGTTATGGCAGTCAATAATCATTTTTTCAATTAATGAATTTATTTCGTGGTCTGAAATATCGATATTATCTCTTTTATATGAGCTTAATGCTTTAGTAAATAAATATCCGAAGTATATGTTTCTACTTTGTTGATATTGTTTAAATTTTTCAATAAATTGCTTATGAATTTCATTTTCTTCTGGGCTTAGAGAAACATTTGCAAAGAGCTTATCTGTATCGATTGCATTTTTTATATATACATTTTCTTTTTTTAATATTAATATCATTTTAATTACAATTAAAATGAATATAGCTAAGAAGATTGCACCAAAAACATAACAAACAATACTTGATTTTTTAGATATAGTTCCCATAATGCCATTTGCTGCTAAAAATAGCAAGATAGCAATAATAAGAAAGAGAGCAACGGTTAGAAAACCAATAATGTTTAATATAACATTTTTTATTTTCATTTTCTTTTCTTTTGATGTCATTATTTAGCCTCCTTATAAGTTATTAGTTGAGGATTTTTTAAATATCCCCTTCCAGCTTTAGCGATATTATGGCCATTCAATCTTGTTGCATCGGCGCTAAAATTTACTCAAATTTTTAGCAACGCTGCACCGATGCCGTATGTGTCAACAGGAGTCTCTTCGGCTTCAAATTCAGCAATTCTTTGAGCGTCAAATCCACTTGAAACAGTTATTTTTACATGCTTTCCGTTATTATTATCTAATGCTTCTCTTAACCTTTTTACTTGATTTGGTGTAACGCCAAATTCCGCTTCACCCTCCTTGAACATTTTATCGATAAGCGCTTTCGATGTGTCTATTCTTACGCTTTTTAAGTCTTTGCCAAAAGCCTTTAAACATTTTAATGAATCGCTTATAACATCGTTGTGAAAATCAACTAGGGCAACTAGATCATCATTTGGAAAAACATTTTTATATGCTTTGCAAGCAGCTACAATATCACCGTCAAACATTTGAATTAATGCATGAGGCATGCTTCCAAAAGTATGCTTTGGATCAAAATTTGAGCTTAGAAAAGTCGAGTGATTTTCTATACCACCTATTCTTATTGCATATGAGTCGTTTGGTTGATTTATATAATGATCTGCCCTATCTGCCATGCTTACGATCTTTTTGCCATTTGCTGCTTTTATTGCTCAATATGCATTGGTTGCTATTGATGATTGCCTTGCTAAAATGCCATCAATGATTCCTTCTCATATACCAAATTCATAATAAGGGCCTTCAAGTTCTAAAACTACTTCTCTATTGTGAATTATACTGCCTTCTGGCATGTATCTTATGGTGTATTTAGAAATATCTGTGTTTTGTTTTAAAATATCGATTGCTTCATTTATTCCACATAATTTGACATCATTATGTCTTTGAAAGAATTGTAGAGTAATGTTTTGATCTTTTTTGAACTTTTCTGATATTTTTTGAGTTTTGTAAAAATAAATTGAAATGTAATCTTTCAAATTGATTTTTTTCATTATTCTCCTTTTTATAATAATTTAATAAATTATTATTAGCCTCTTATTAAAATTTTAAACAAAATTAATTAATAAAATCATATAAAGTTATAAAATAATAAATCATTGTTAAAATATCTTTTTTAAAGACATGTTTTATAATAAAGGAGAATAAGTATGGCAAAAAACCAAGATAAAAAAATGCTATCTCCAGAAGAAAAAGAAATAGAAAATATAAAGAAAATATCAAAAAATGGTTGACATTTTAGTTATTGGTCGTTATTTTTTATTTTAGGAGTTATAGCTATTTTAATATGAGCAGTTGAATCTAAAAGTTCATCTTCAATGGTAAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCAATGATAGCTGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCATTAAGAAAATGCTATTTGATTTCGCTAATTCCATTATTGTTTTTTGTAGCATTGGTAGCAAGCTTTGTATCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAACGTTGCTATAGCAGGATAGAAAAAAACCTTGAATTAATTTTCAAGGTTTTTTTGTTTAATTTCTTTTGTTATTTTGTTTGCATTTCTTTGGCGATGAGTAAATAGAATTATATAAAATACTAATGTAGGAATGGCAATGCTTATAAAGATTATAAAAAAGCAAGATAAGGCTATGTAACCATTTTGATTTCATAGGATGAAATAATTATCCAATGAAGTTATAGCATAAACACTTAATGTTGGAATAAACGCTATTGCAACTTGCGAAATTAAAGTGTGGCGTTTAGTTATTAATTGTGGCTTTTTCATCAATAGTATTGACGTTGTTAATATGTATGTAATTAAAATATAACCAACAATTACAAGTATTATAGGTAAAGTAATTTTCATAGTTAAAATTTTACTCCATTAAAAAATAAGAGCATTTAAAAAGCACATGTTTTAAACATGGCTTTTTAAAATTAG", "length": 750518, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_013368015.1|CP055149.1", "orig_description": "HMT-632 Metamycoplasma hominis SP10291" } ], "run": { "start": "2026-01-28 09:36:38", "end": "2026-01-28 09:44:35", "duration": "7.95 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }