{ "genome": { "genus": "Metamycoplasma", "species": "hominis", "strain": "SP3615", "taxon": "Metamycoplasma hominis SP3615", "complete": true, "gram": "?", "translation_table": 11 }, "stats": { "size": 715990, "gc": 0.27096188494252715, "n_ratio": 0.0, "n50": 715990, "n90": 715990, "coding_ratio": 0.7368301233257448 }, "features": [ { "type": "cds", "sequence": "contig_1", "start": 472, "stop": 621, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAGTCAAGCAAAAATGGAAGAATTCAACCAAAAGAAAGCCAAAATTGAATCCAAATATGCTGCTTACAAAGGCGACAGACAAATGATGCAACGTAAGCAAGCTGAAATTAGTGAATTATATAAGAAAGAAAAAATTTCTCCTTGA", "aa": "MFSQAKMEEFNQKKAKIESKYAAYKGDRQMMQRKQAEISELYKKEKISP", "aa_hexdigest": "7588ca6ca96a15cae5b57833b385386d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5875.7765, "isoelectric_point": 9.646807289123537 }, "id": "GLJLFKCOHC_45", "locus": "GCA013368035_00001" }, { "type": "cds", "sequence": "contig_1", "start": 1300, "stop": 1434, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATGAAAAGGGAAATTACAAAACCAAAAATTACACTCCATTAACCAAATTCAGTGAAATTTTAATTCCTAATTCTTTATCGTTTGACACTAAGCCAAAAGATAAGGATGGCATTATACCAACAGATTGA", "aa": "MGDEKGNYKTKNYTPLTKFSEILIPNSLSFDTKPKDKDGIIPTD", "aa_hexdigest": "00a76826a52447d9c1fa25fe72451b42", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4961.553500000002, "isoelectric_point": 6.104928398132324 }, "id": "GLJLFKCOHC_46", "locus": "GCA013368035_00002" }, { "type": "cds", "sequence": "contig_1", "start": 1513, "stop": 1854, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTATCGGTTTAGTTGGTTGTGTACAATCATTTACTGTAAAATCAAGTAATAAAGTCGGTTCTGGACAAGAGCTATATTCTAAAAAGAGTGACATCTCACCAAATATTGTCACTTATAGATACGATAGCACTGGTAATTTCTTTACCTTACCAAACAAAATAAATGATAAAAATATTATCGCCAATACATATCTTGCTTTAAAAGAACCTAATGCTATTAATGAGTTAAGGAAGAGAGATCCTAATTATGGTGCATATGGTTCTCGTTCATTTGGATTGCAATTGCAAATGCCAAAAAACGACAAAGCAAAAGTTTTATCTTCTCATGATGAAGATTGA", "aa": "MAIGLVGCVQSFTVKSSNKVGSGQELYSKKSDISPNIVTYRYDSTGNFFTLPNKINDKNIIANTYLALKEPNAINELRKRDPNYGAYGSRSFGLQLQMPKNDKAKVLSSHDED", "aa_hexdigest": "200f4ab9d28dac35760f2bb7d475a211", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12536.983699999986, "isoelectric_point": 9.211774635314942 }, "id": "GLJLFKCOHC_47", "locus": "GCA013368035_00003" }, { "type": "cds", "sequence": "contig_1", "start": 1970, "stop": 2230, "strand": "-", "frame": 1, "gene": "rnpA", "product": "ribonuclease P protein component", "db_xrefs": [ "COG:COG0594", "COG:J", "EC:3.1.26.5", "GO:0000049", "GO:0004526", "GO:0008033", "SO:0001217", "UniRef:UniRef50_A0A448ZZD2", "UniRef:UniRef90_D1J7D9" ], "nt": "GTGATTTCAAGAAATTTAATAATTTATTTTAACAAATCCGATTTCTTTAAAGTGGGTATTTCAATCCCTAAGCAATTTGCAAATGCTGTTTTGAGAAATCATTACAAAAATCAAATCAGATCAATTCTAAGAAACATTGATTTTAGCACTTTAAATTACGAAACTGTGATTATTGCTCGCAAAGGCTTCTTGAGTCTTGACTGGAATCAAAAAGTTAAAGAAGTAAAAAAACTTTATGAAAGGATCGCTGATGGCAAATAA", "aa": "MISRNLIIYFNKSDFFKVGISIPKQFANAVLRNHYKNQIRSILRNIDFSTLNYETVIIARKGFLSLDWNQKVKEVKKLYERIADGK", "aa_hexdigest": "6452ad081c7390eca0bc21ce527ec9ca", "start_type": "GTG", "rbs_motif": null, "genes": [ "rnpA" ], "truncated": "3-prime", "pseudogene": { "start": 1970, "stop": 2299, "observations": { "mutation": [ 2266 ], "stop": [ 2266 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7D9", "query_cov": 1.0, "subject_cov": 0.7889908256880734, "identity": 0.965, "score": 169.0, "evalue": 6.59e-53, "gene_start": 1, "gene_end": 86, "reference_start": 24, "reference_end": 109, "reference_sequence": "MINKNNVVKKNWEFQKILDSHNQVVSKNLIIYFNKSDFFKVGISIPKQFANAVLRNHYKNQIRSILRNIDFSTLNYETVIIARKGFLSLDWNQKVKEVKKLYERIADGK" }, "paralog": false, "identity": 0.9724770642201835, "score": 213.0, "evalue": 7.14e-73, "description": "Internal stop codon at 2266. Nonsense mutation around 2266.", "psc": { "uniref90_id": "UniRef90_D1J7D9", "gene": "rnpA", "product": "ribonuclease P protein component", "ec_ids": [ "3.1.26.5" ], "uniref50_id": "UniRef50_A0A448ZZD2", "cog_id": "COG0594", "cog_category": "J", "go_ids": [ "GO:0000049", "GO:0004526", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7D9", "EC:3.1.26.5", "UniRef:UniRef50_A0A448ZZD2", "COG:COG0594", "COG:J", "GO:0000049", "GO:0004526", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZD2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZD2" ], "product": "Ribonuclease P protein component" } }, "id": "GLJLFKCOHC_48", "locus": "GCA013368035_00004" }, { "type": "cds", "sequence": "contig_1", "start": 2327, "stop": 2473, "strand": "-", "frame": 1, "gene": "rpmH", "product": "50S ribosomal protein L34", "db_xrefs": [ "COG:COG0230", "COG:J", "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904", "KEGG:K02914", "RefSeq:WP_012855284.1", "SO:0001217", "UniParc:UPI00006920BF", "UniRef:UniRef100_D1J7E0", "UniRef:UniRef50_P05647", "UniRef:UniRef90_B3PNJ7" ], "nt": "ATGAAAAGAACATACCAACCAAAAAAACGCAAACACATTAAAGTTCATGGTTTTATGGCTAGAATGCAAACTAAAAATGGTAGAAAAGTTTTAGCAGCTAGACGTGCAAAAGGTAGATATACATTAACTGTATCAGACAATAAATAA", "aa": "MKRTYQPKKRKHIKVHGFMARMQTKNGRKVLAARRAKGRYTLTVSDNK", "aa_hexdigest": "c12d043f3061d758a33db3c492b0b7ea", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00006920BF", "ncbi_nrp_id": "WP_012855284.1", "uniref100_id": "UniRef100_D1J7E0", "db_xrefs": [ "SO:0001217", "UniParc:UPI00006920BF", "RefSeq:WP_012855284.1", "UniRef:UniRef100_D1J7E0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E0", "uniref90_id": "UniRef90_B3PNJ7" }, "psc": { "uniref90_id": "UniRef90_B3PNJ7", "gene": "rpmH", "product": "50S ribosomal protein L34", "uniref50_id": "UniRef50_P05647", "cog_id": "COG0230", "cog_category": "J", "kegg_orthology_id": "K02914", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P05647", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P05647" ], "product": "Large ribosomal subunit protein bL34" }, "genes": [ "rpmH" ], "id": "GLJLFKCOHC_49", "locus": "GCA013368035_00005" }, { "type": "oriC", "sequence": "contig_1", "start": 2474, "stop": 2804, "strand": "?", "product": "origin of replication", "nt": "AACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGTATATAAAATACCTAAGAAAAAAAATTTAATATTTTTTATGTTTTTAATGTAAATTAAAAGAAACATAAAATTTAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAAACCTCTTATTTTAAAAACAAAAGCCCTAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGCTTATTAATTTATTAATTTTGAATTTTAAAATATAATAGAGGT", "id": "GLJLFKCOHC_1228" }, { "type": "cds", "sequence": "contig_1", "start": 2805, "stop": 3734, "strand": "+", "frame": 3, "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "db_xrefs": [ "COG:COG0593", "COG:L", "KEGG:K02313", "SO:0001217", "UniRef:UniRef50_A0A448ZWT6", "UniRef:UniRef90_D1J7E1" ], "nt": "ATGAAAACTACAAATCCCTCTGAATTAGATTTAAGAACACATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCTTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGACGTTTTAACATATATTAAGAACAGTCATAATGAAAGTATTAAAAGAGCAATTAAAAACGTTTATGGTGAAGAATATAATTTGGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTACAAGATAGCAATAATGTTGAATCTCCAACTAATTCATTGACTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAACATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTTATTCATGCTTCAAGTGGGCTTGGTAAAACACATCTATTACATGCTATCGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTGATGTCTTATGATTGTCTAATGTTTGATGATGTTCAACAATATGGTAATCGAGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAACCGGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATGTCTTGAAATTTAAATTGCAAGAAAACGGTATAAATCCGGAACTTTGA", "aa": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESPTNSLTNNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINVLKFKLQENGINPEL", "aa_hexdigest": "a5db714504fa377c389873082fcd119e", "start_type": "ATG", "rbs_motif": null, "genes": [ "dnaA" ], "truncated": "3-prime", "pseudogene": { "start": 2805, "stop": 4037, "observations": { "mutation": [ 3732 ], "stop": [ 3732 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E1", "query_cov": 1.0, "subject_cov": 0.6673866090712743, "identity": 0.99, "score": 600.0, "evalue": 2.35e-214, "gene_start": 1, "gene_end": 309, "reference_start": 1, "reference_end": 309, "reference_sequence": "MKTTNPSELDLRTHNMSFQKELVNTASDPLIYDQFLSSLEIVKENNKMIYILVKGPEDVLTYIKNSHNESIKRAIKNVYGEEYNLVYIRNLDEIKSLQDSNNVESATNSLANNSKENFSNIKKNLTFDDYAIGKFNNMALKAAKAICNSEKILFSPLFIHASSGLGKTHLLHAIGNELLKHGRSALYINPDSLTRRLVEQLKSKNQEQINKIIDELMSYDCLMFDDVQQYGNRDSTLNVLFNIIDNMIMNDKQIIFCGDKKPDDLGGFEQRFITRFNGGLTVEILKPELNDVINILKFKLQENGINPELWEEESLKFIARNFSSSIRNIEGAINRIKLFQDGDDGFFTYDINTIKSIFNSMTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLNWIEKNANSNPTLKLALQKIESNLKKIS" }, "paralog": false, "identity": 0.9878048780487805, "score": 800.0, "evalue": 2.48e-295, "description": "Internal stop codon at 3732. Nonsense mutation around 3732.", "psc": { "uniref90_id": "UniRef90_D1J7E1", "gene": "dnaA", "product": "chromosomal replication initiator protein DnaA", "uniref50_id": "UniRef50_A0A448ZWT6", "cog_id": "COG0593", "cog_category": "L", "kegg_orthology_id": "K02313", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E1", "UniRef:UniRef50_A0A448ZWT6", "COG:COG0593", "COG:L", "KEGG:K02313" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWT6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWT6" ], "product": "Chromosomal replication initiator protein DnaA" } }, "id": "GLJLFKCOHC_50", "locus": "GCA013368035_00006" }, { "type": "cds", "sequence": "contig_1", "start": 3885, "stop": 4115, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08299.17" ], "nt": "ATGACTCAAGTTGCCGAGAATATTACTCCTGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGATGTGGTTAATGCTCGCAGAATAGCAACATATTTAGTAAATAAAAATTTTAATTATACATTGCAAGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCGCTTAATTGA", "aa": "MTQVAENITPDKIIDAVSKYYKIDRKKILSNVRTNDVVNARRIATYLVNKNFNYTLQEIGKVVGNQSHSNVIVSLN", "aa_hexdigest": "28ae5fe87d266549f489ee290f7117cb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08299.17", "name": "Bac_DnaA_C", "length": 66, "aa_cov": 0.868421052631579, "hmm_cov": 1.0, "evalue": 2.323607120532523e-21, "score": 71.04753875732422, "start": 10, "stop": 75 } ], "seq_stats": { "molecular_weight": 8637.7939, "isoelectric_point": 9.753437995910645 }, "id": "GLJLFKCOHC_51", "locus": "GCA013368035_00007" }, { "type": "cds", "sequence": "contig_1", "start": 4212, "stop": 5309, "strand": "+", "frame": 3, "gene": "dnaN", "product": "DNA polymerase III subunit beta", "db_xrefs": [ "COG:COG0592", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360", "KEGG:K02338", "RefSeq:WP_175949764.1", "SO:0001217", "UniParc:UPI001588828C", "UniRef:UniRef100_UPI001588828C", "UniRef:UniRef50_A0A2Z4LLI0", "UniRef:UniRef90_D1J7E2" ], "nt": "ATGAAATTAAAAATTGATAAATTATTATTAGATAATGCAATTGAAAGAGTATCAAAATCTGTGGACGCAAATCCGTTCTTGCCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAACACGAAATTGAAACATCAATTGATGCTCAAATTGAATTGCCAGGAATTGTATTAGTTGAACTTAGCTTGTTTAAAAATATTATTAAGAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATTAGTACAAATAATGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATCGATTTTGCATGTTATGGGGAACAAGTAAAAATTAACTGGAACGATTTTAAATCTTTAGTTCGTGATGTTATTTTTGCCGCTTCAACAAACGAAGTTAGTCTAATCTTGTGCTGTGTCAATATATCGGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCTCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAACTTTGATTACAATGGCGACGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAGTCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACCGAACTTAAAATAACTAAAAAAGAACTAAATAACTTGTTAAATAAGGCATCAGTTATTATTAATGAAAACTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAATCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGTGAAGAATTGAAACTCGCATTGAATAGTAGATTCTTAAAGGATGCTATTTCTGTGTATGAAGATAATTTGAATATTTTATTTACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAA", "aa": "MKLKIDKLLLDNAIERVSKSVDANPFLPVMKGILIQAEDNKITLIGSNGETSIKHEIETSIDAQIELPGIVLVELSLFKNIIKKLDGEIVLEVVNNILKISTNNENYNLNIYDVNEYIAIDFACYGEQVKINWNDFKSLVRDVIFAASTNEVSLILCCVNISAANKKIKFITTDRFRYAQAVQDISEDVTFNISILAKNLRDITNFDYNGDVILNISDQKISFKLDSTTIESKVVDQLYQDVSRIVPQEFDTELKITKKELNNLLNKASVIINENYNKIKLHIVDNVLIISSAREEIANAEIKTNNFSYNGEELKLALNSRFLKDAISVYEDNLNILFTKDKLRIVIKSDSNPNVLQLITPQKGF", "aa_hexdigest": "b64a4b5f01e6656a504614b60976eafe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI001588828C", "ncbi_nrp_id": "WP_175949764.1", "uniref100_id": "UniRef100_UPI001588828C", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588828C", "RefSeq:WP_175949764.1", "UniRef:UniRef100_UPI001588828C" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588828C", "uniref90_id": "UniRef90_D1J7E2" }, "psc": { "uniref90_id": "UniRef90_D1J7E2", "gene": "dnaN", "product": "DNA polymerase III subunit beta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A2Z4LLI0", "cog_id": "COG0592", "cog_category": "L", "kegg_orthology_id": "K02338", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LLI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LLI0" ], "product": "DNA polymerase III subunit beta" }, "genes": [ "dnaN" ], "id": "GLJLFKCOHC_52", "locus": "GCA013368035_00008" }, { "type": "cds", "sequence": "contig_1", "start": 5309, "stop": 5482, "strand": "+", "frame": 2, "gene": "ybcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "db_xrefs": [ "COG:COG2501", "COG:J", "SO:0001217", "UniRef:UniRef50_N9UZE0", "UniRef:UniRef90_D1J7E3" ], "nt": "ATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGTCCAACAGGCGGAATGGCAAAATATTTTGTTCAGGTTCACAGAATATTGATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGA", "aa": "MEIEITGEFIKLSQFLKKIDVCPTGGMAKYFVQVHRILINGNLANGRNAKIYPGDTV", "aa_hexdigest": "c366bbf7c6e153c1c5ccd4e3f3610b33", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7E3", "query_cov": 1.0, "subject_cov": 0.8142857142857143, "identity": 1.0, "score": 119.0, "evalue": 4.16e-34, "valid": true, "gene": "YbcJ", "product": "Ribosome-associated protein YbcJ, S4-like RNA-binding protein", "uniref50_id": "UniRef50_N9UZE0", "cog_id": "COG2501", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_N9UZE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZE0" ], "product": "RNA-binding S4 domain-containing protein" }, "genes": [ "ybcJ" ], "id": "GLJLFKCOHC_53", "locus": "GCA013368035_00009" }, { "type": "cds", "sequence": "contig_1", "start": 5590, "stop": 5787, "strand": "+", "frame": 1, "gene": "rpmB", "product": "50S ribosomal protein L28", "db_xrefs": [ "COG:COG0227", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855288.1", "SO:0001217", "UniParc:UPI0001BC1FC5", "UniRef:UniRef100_D1J7E4", "UniRef:UniRef50_Q98PM6", "UniRef:UniRef90_B3PLS0" ], "nt": "ATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAG", "aa": "MPGRDQLTGQKALSGNKRSHALNTAKRTFDLNLQKVTVLTETGSKKTVRVTAKNARTLKKYGLVA", "aa_hexdigest": "be79e6e221684802b48e19dfde4c6d71", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC1FC5", "ncbi_nrp_id": "WP_012855288.1", "uniref100_id": "UniRef100_D1J7E4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FC5", "RefSeq:WP_012855288.1", "UniRef:UniRef100_D1J7E4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7E4", "uniref90_id": "UniRef90_B3PLS0" }, "psc": { "uniref90_id": "UniRef90_B3PLS0", "gene": "rpmB", "product": "50S ribosomal protein L28", "uniref50_id": "UniRef50_Q98PM6", "cog_id": "COG0227", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PM6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PM6" ], "product": "Large ribosomal subunit protein bL28" }, "genes": [ "rpmB" ], "id": "GLJLFKCOHC_54", "locus": "GCA013368035_00010" }, { "type": "cds", "sequence": "contig_1", "start": 5998, "stop": 6153, "strand": "+", "frame": 1, "gene": null, "product": "Core-binding (CB) domain-containing protein", "db_xrefs": [ "RefSeq:WP_175949765.1", "SO:0001217", "UniParc:UPI0015885065", "UniRef:UniRef100_UPI0015885065", "UniRef:UniRef50_A0A6A8Q154", "UniRef:UniRef90_UPI0015885065" ], "nt": "ATGAGAAATATAGATCAATTAGAAATCTTTTATGCTGGATTTGAAACGAAGGTTCAAACACATAACAGAAGTGCTAAAACACTTAAAAATTATACATTTGTTTTAAGACAATTAAGAGACTGGGACACCCCTCGAAGAATTAATAGAATAAATTAA", "aa": "MRNIDQLEIFYAGFETKVQTHNRSAKTLKNYTFVLRQLRDWDTPRRINRIN", "aa_hexdigest": "2ecbd919ce8f8604eee55ccf949d54a8", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0015885065", "ncbi_nrp_id": "WP_175949765.1", "uniref100_id": "UniRef100_UPI0015885065", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015885065", "RefSeq:WP_175949765.1", "UniRef:UniRef100_UPI0015885065" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015885065", "uniref90_id": "UniRef90_UPI0015885065" }, "psc": { "uniref90_id": "UniRef90_UPI0015885065", "uniref50_id": "UniRef50_A0A6A8Q154" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q154" ], "product": "Core-binding (CB) domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_55", "locus": "GCA013368035_00011" }, { "type": "cds", "sequence": "contig_1", "start": 6183, "stop": 6350, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAATACAAACGTAAAACTTTATTAAATAATTTTCCAAGCAATTTAAAACAAAAAATTTCTGAATTTTTAAAAAGCAATGAACTAGATTTAAATATAAGAGCTGAAAAAATTACAAAAGAAATTTCTATTAATTTATTCAAATTTATAAACAATAAAAATTAA", "aa": "MFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN", "aa_hexdigest": "d061f8f47610aed152b4b48fc3d66d05", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6618.659699999998, "isoelectric_point": 9.996741676330569 }, "id": "GLJLFKCOHC_56", "locus": "GCA013368035_00012" }, { "type": "cds", "sequence": "contig_1", "start": 6387, "stop": 6956, "strand": "-", "frame": 3, "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "db_xrefs": [ "COG:COG0030", "COG:J", "EC:2.1.1.182", "GO:0000179", "GO:0006364", "SO:0001217", "UniRef:UniRef50_D1J7E5", "UniRef:UniRef90_D1J7E5" ], "nt": "ATGATTAAAGCATTAAAATCATTGGGCCAAAATTTCTTAAGCAATAAAAACATTCAAAAAGAGATTGTAAAGGCTGCAAACGTTGCGGGCAAAAATATTATTGAAATTGGCCCAGGAATGGGCGCAATAACTGATCAAATGGCAGATATTGTAAATAGACTTGTATGTATTGAATTTGATAAAAGATTATACGAATTTTTATTACAAAAAAATTATTCAAGCAATGTTGAAATATTGAACCAAGATTTCTTACAAACAGATTTATTAAAATATACTGATTTTGAAGTAATTGGAAACATTCCTTACAATATAACAAGCGATATAATTTTTAAATTATTAGATAACGCTAAAAACATTAATAAAATAACATTAATGGTTCAAAAAGAAGTAGCAGATAGAATATGCTGTGGAGCGGGCAATTCTCAATATAGTAAATTAGCGGCATCAATACAACTTCTCTATGAACCTAAATATTTATTCACTGTTAAGGCAAAAGAATTTAATCCCGCTCCTAAAGTGGATTCGGCAGTTATTCAACTGAATCTAATAAAGAATAATGATTTTATTTGA", "aa": "MIKALKSLGQNFLSNKNIQKEIVKAANVAGKNIIEIGPGMGAITDQMADIVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAKNINKITLMVQKEVADRICCGAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLNLIKNNDFI", "aa_hexdigest": "67ca3b9c51e5610eb304ebe95996878d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rsmA" ], "truncated": "5-prime", "pseudogene": { "start": 6183, "stop": 6980, "observations": { "mutation": [ 6389 ], "stop": [ 6389 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7E5", "query_cov": 1.0, "subject_cov": 0.7132075471698113, "identity": 0.9840000000000001, "score": 365.0, "evalue": 1.18e-126, "gene_start": 1, "gene_end": 189, "reference_start": 9, "reference_end": 197, "reference_sequence": "MLERFLKSMIKALKSLGQNFLSNKNIQKEIVKAANVAGKNIIEIGPGMGAITDQMADVVNRLVCIEFDKRLYEFLLQKNYSSNVEILNQDFLQTDLLKYTDFEVIGNIPYNITSDIIFKLLDNAENINKITLMVQKEVADRICCGAGNSQYSKLAASIQLLYEPKYLFTVKAKEFNPAPKVDSAVIQLILIKNNDFIWNNQVEILKFIKQMFQYKRKTLLNNFPSNLKQKISEFLKSNELDLNIRAEKITKEISINLFKFINNKN" }, "paralog": false, "identity": 0.9849056603773585, "score": 508.0, "evalue": 3.8e-185, "description": "Internal stop codon at 6389. Nonsense mutation around 6389.", "psc": { "uniref90_id": "UniRef90_D1J7E5", "gene": "rsmA", "product": "16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase RsmA", "ec_ids": [ "2.1.1.182" ], "uniref50_id": "UniRef50_D1J7E5", "cog_id": "COG0030", "cog_category": "J", "go_ids": [ "GO:0000179", "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7E5", "EC:2.1.1.182", "UniRef:UniRef50_D1J7E5", "COG:COG0030", "COG:J", "GO:0000179", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7E5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7E5" ], "product": "Ribosomal RNA small subunit methyltransferase A" } }, "id": "GLJLFKCOHC_57", "locus": "GCA013368035_00013" }, { "type": "cds", "sequence": "contig_1", "start": 6949, "stop": 7716, "strand": "-", "frame": 2, "gene": "tatD", "product": "YchF/TatD family DNA exonuclease", "db_xrefs": [ "COG:COG0084", "COG:N", "EC:3.1.21.-", "KEGG:K03424", "RefSeq:WP_175949767.1", "SO:0001217", "UniParc:UPI001588A61E", "UniRef:UniRef100_UPI001588A61E", "UniRef:UniRef50_N9U095", "UniRef:UniRef90_A0A6A8Q279" ], "nt": "ATGAAATTTATTGATTTACACACCCACCCATCAACCGAATACTATTCAGATCCTCTTGAAATAGTTAATGAATGGCATAACCTAAATATGGAAAAGCTTTTTGTATGTGGAACAAACGAAGATGATTCGATACAAGCAATAGAACTTGCTAAAAAGGTTGATTTTGTTTATCCTGTTATTGGAATACATCCAACATTATCAAATGGTTTGCAAGATAGAGAAATTTTAAACAAGATATATGAACCAAATGTTGTTGCAATTGGAGAAGTTGGCCTTGATTATCATTATGATGATTCGCCTAGCAAAGAAATTCAAAAACAAGGATTTATTGCCCAAATAGATTTTGCAAAAGAACATAATTTACCAGTAATTATGCACATTAGAGATGCTTTAGATGATTCTTTAGATATAATAACCTTGCCTCAATATAAAGACATTAATTTTATATTTCACTCATTTAGTGGGGATCTTAGTTTTTTAAATAAATGCTTGACATATTCAAATATCTATTATTCAATAAGCGGGGTTGTTACTTTTAAAAATGCTAAGCCTTTACAAGAAGCAGTAAAAAATATTCCTTTAGATAGAATATTTTGTGAAACTGATACACCTTATTTAGCCCCAACTCCAATGAGAGGAAAACCCAACATTAGCCCTTTTGTTCAATACACATATGAATATATTGCTAATTTAAAACAAATTGATGTTGATGTTTTAGTTGAACAAATTAAAAAGAATGTTAGAAAGGTTTTTAAAATCTATGATTAA", "aa": "MKFIDLHTHPSTEYYSDPLEIVNEWHNLNMEKLFVCGTNEDDSIQAIELAKKVDFVYPVIGIHPTLSNGLQDREILNKIYEPNVVAIGEVGLDYHYDDSPSKEIQKQGFIAQIDFAKEHNLPVIMHIRDALDDSLDIITLPQYKDINFIFHSFSGDLSFLNKCLTYSNIYYSISGVVTFKNAKPLQEAVKNIPLDRIFCETDTPYLAPTPMRGKPNISPFVQYTYEYIANLKQIDVDVLVEQIKKNVRKVFKIYD", "aa_hexdigest": "bb21ab66590fc298e7df5c3f000c5d4a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI001588A61E", "ncbi_nrp_id": "WP_175949767.1", "uniref100_id": "UniRef100_UPI001588A61E", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588A61E", "RefSeq:WP_175949767.1", "UniRef:UniRef100_UPI001588A61E" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588A61E", "uniref90_id": "UniRef90_A0A6A8Q279" }, "psc": { "uniref90_id": "UniRef90_A0A6A8Q279", "gene": "TatD", "product": "YchF/TatD family DNA exonuclease", "ec_ids": [ "3.1.21.-" ], "uniref50_id": "UniRef50_N9U095", "cog_id": "COG0084", "cog_category": "N", "kegg_orthology_id": "K03424" }, "pscc": { "uniref50_id": "UniRef50_N9U095", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U095" ], "product": "TatD DNase family protein" }, "genes": [ "tatD" ], "id": "GLJLFKCOHC_58", "locus": "GCA013368035_00014" }, { "type": "cds", "sequence": "contig_1", "start": 7914, "stop": 8249, "strand": "-", "frame": 3, "gene": "mtnN", "product": "Nucleoside phosphorylase domain-containing protein", "db_xrefs": [ "COG:COG0775", "COG:FH", "SO:0001217", "UniRef:UniRef50_A0A454C9F7", "UniRef:UniRef90_A0A454C9F7" ], "nt": "TTGTTAGCGCACTGTGGAGTTGGAAAAACTAATTCTGCTTTAATGTTAAGTTATTTATTGACTAAATTCAATATATTAAAAGTAATTAACATTGGACCTGCTATATCAATTTTTAATGGTTGTATTGGAGATATTTATTGCATACAAAATTGCAAATATTATGACGTTAATTTAACTGCCTTACCTAATTATAAAATCGGGCAATTGCCTAATCTGCAAGATGTATTTTATTGCAATCAAGATCTAAAAATTTGCAATAATTTTAAAAAAGCCAATATTATAACCGGTGATAGTTTTATTAACAAAAGCATAATAAATTCAAATATATTAAATTGA", "aa": "MLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGCIGDIYCIQNCKYYDVNLTALPNYKIGQLPNLQDVFYCNQDLKICNNFKKANIITGDSFINKSIINSNILN", "aa_hexdigest": "bd91378164adbee8804865bd09de6553", "start_type": "TTG", "rbs_motif": null, "genes": [ "mtnN" ], "truncated": "both", "pseudogene": { "start": 7716, "stop": 8402, "observations": { "mutation": [ 7766, 7916, 8267 ], "stop": [ 7766, 7916, 8267 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9F7", "query_cov": 0.990990990990991, "subject_cov": 0.4824561403508772, "identity": 0.9359999999999999, "score": 214.0, "evalue": 1.28e-68, "gene_start": 1, "gene_end": 110, "reference_start": 52, "reference_end": 161, "reference_sequence": "MEKKFYLFIFAEPLETEQLRIKYSKHLKQIKIDFENNFQNLYELNWNNKQILLAHCGVGKTNSALMLSYLLTKFNILKVINIGPAISIFNGSVGDIYCIQNCKYYDVNLTALPNYKIGQLPSLQDVFHCNQDLKICSNFKKANIITGDSFINKSIINSDILDWNTNNLLGDMECCSLAHTSFIFGKPFYSFKVISDCLESNNNLEYRQSKAIWELKIIDIFKDILGEL" }, "paralog": false, "identity": 0.9473684210526315, "score": 440.0, "evalue": 3.07e-160, "description": "Internal stop codon at 7766, 7916, 8267. Nonsense mutation around 7766, 7916, 8267.", "psc": { "uniref90_id": "UniRef90_A0A454C9F7", "gene": "MtnN", "product": "Nucleoside phosphorylase domain-containing protein", "uniref50_id": "UniRef50_A0A454C9F7", "cog_id": "COG0775", "cog_category": "FH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9F7", "UniRef:UniRef50_A0A454C9F7", "COG:COG0775", "COG:FH" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9F7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9F7" ], "product": "Nucleoside phosphorylase domain-containing protein" } }, "id": "GLJLFKCOHC_59", "locus": "GCA013368035_00015" }, { "type": "cds", "sequence": "contig_1", "start": 8265, "stop": 8402, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGAAATTTTATTTATTTATTTTTGCAGAACCACTAGAAACTGAACAATTAAGACTAAAATATAACAAGCATTTAAAACAAATAAAAATTGATTTTGAAAACAATTTTCAAAATCTTTATGAATTAAATTGA", "aa": "MEKKFYLFIFAEPLETEQLRLKYNKHLKQIKIDFENNFQNLYELN", "aa_hexdigest": "3a72260bc2d88e82e977ff4ee9401616", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5682.503699999999, "isoelectric_point": 6.5483293533325195 }, "id": "GLJLFKCOHC_60", "locus": "GCA013368035_00016" }, { "type": "cds", "sequence": "contig_1", "start": 8714, "stop": 8839, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGAAGGCAAATATAAGGTTTTAAACGACATTCTATTGCAAATTGATAATGATAAAACAGCTCAAATAGATCATATTGTTGTTTCAACTTACGGTATTTTTGTAATTGAAACAAAGAATTGA", "aa": "MNEGKYKVLNDILLQIDNDKTAQIDHIVVSTYGIFVIETKN", "aa_hexdigest": "fc16c759b92a451d72e0b936a87dbd2a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4694.318299999999, "isoelectric_point": 4.889369773864746 }, "id": "GLJLFKCOHC_61", "locus": "GCA013368035_00017" }, { "type": "cds", "sequence": "contig_1", "start": 8912, "stop": 9367, "strand": "+", "frame": 2, "gene": null, "product": "nuclease-related domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8U8", "UniRef:UniRef90_UPI000695A672" ], "nt": "ATGAAGAACCCAATAAAACAAAATGAACGACATATTGAAGCATTAAAGCAGATATTTTCAAAATACAATATAAATATAAATAAAGAATTATTTTATTCTCTTATTGTATTTGTCCAAGGCCAAAAGCCCAAAATTGATGATCTGAATAATTGCTCTGTAATACCTCTCAATGATTTAGTAAAAAAAATTCATTCATACAATAAAGAAATATTAGAAGAATCTCAAATTAAAGAACTTATAGAATTGATTCAACAAGAAAATGTTGAAGATATACAAACAAGGTTAGAACACAACGAACGTGCAAAAAAAGTTTTACAAGTTCAACAAGATAAAATTAAAGAACATATTTGTCCACTATGCAATGGCGAACTTACATATAAAAATGGTTTACACGGCAAGTTTTATGGTTGCTCTAATTATCCAAAATGTCATTTTTCAATAGAAAATATTGATTAA", "aa": "MKNPIKQNERHIEALKQIFSKYNININKELFYSLIVFVQGQKPKIDDLNNCSVIPLNDLVKKIHSYNKEILEESQIKELIELIQQENVEDIQTRLEHNERAKKVLQVQQDKIKEHICPLCNGELTYKNGLHGKFYGCSNYPKCHFSIENID", "aa_hexdigest": "0bb86670c541694065f9e9bc9d175ceb", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 8612, "stop": 9367, "observations": { "mutation": [ 8837, 8876 ], "stop": [ 8837, 8876 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000695A672", "query_cov": 1.0, "subject_cov": 0.5392857142857143, "identity": 0.894, "score": 273.0, "evalue": 1.39e-90, "gene_start": 1, "gene_end": 151, "reference_start": 130, "reference_end": 280, "reference_sequence": "MWILEIVISVLIWIVIIVGIAFAIHKKSSRHNYNKTINLIKRDSSKQQKGNDGEFLVAWKLTSLNEGKYKVLNDILLQIDNDKTAQIDHIVVSTYGIFVIETKNWNGFITGGRDSYFWFKRNSGNNIEIMKNPIKQNEWHIEALKQIFSKYNININKELFYSLIVFVQGQKPKIGNLNNCSVIPFNDLVKRIHSYNKEILEESQIENLIKFIQQENIEDKETRLEHNERAKKVLQVQQDKIKEHICPKCNCKLIYKNGLHGKFYGCSNYPKCHFSIENID" }, "paralog": false, "identity": 0.9163346613545816, "score": 464.0, "evalue": 2.42e-168, "description": "Internal stop codon at 8837, 8876. Nonsense mutation around 8837, 8876.", "psc": { "uniref90_id": "UniRef90_UPI000695A672", "product": "nuclease-related domain-containing protein", "uniref50_id": "UniRef50_A0A454C8U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000695A672", "UniRef:UniRef50_A0A454C8U8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C8U8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8U8" ], "product": "NERD domain-containing protein" } }, "id": "GLJLFKCOHC_62", "locus": "GCA013368035_00018" }, { "type": "tRNA", "sequence": "contig_1", "start": 9487, "stop": 9573, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(taa)", "amino_acid": "Leu", "anti_codon": "taa", "score": 74.5, "nt": "GCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 9520, 9522 ], "id": "GLJLFKCOHC_1", "locus": "GCA013368035_00019" }, { "type": "tRNA", "sequence": "contig_1", "start": 9579, "stop": 9654, "strand": "+", "gene": "trnK", "product": "tRNA-Lys(ttt)", "amino_acid": "Lys", "anti_codon": "ttt", "score": 80.1, "nt": "GCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 9611, 9613 ], "id": "GLJLFKCOHC_2", "locus": "GCA013368035_00020" }, { "type": "cds", "sequence": "contig_1", "start": 9892, "stop": 10077, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17676.7" ], "nt": "TTGAAACCTAATGAACTATGTGATAAGGTTTTATTTCTTGAAACAAGTGAAGAAAAGCCAAGCCCACAATTACTTAAAAAGATGTTGCTAAAATTCAAAGAAGTTGGCTTATTTAAAAATATTCAAGGAATATTAATCGGATGCCCTCAAAATCGTGTTTATTATAATGAATATAAAGAAGTTTGA", "aa": "MKPNELCDKVLFLETSEEKPSPQLLKKMLLKFKEVGLFKNIQGILIGCPQNRVYYNEYKEV", "aa_hexdigest": "b15eabb9ad23773cd2413a12e5e79506", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17676.7", "name": "Peptidase_S66C", "length": 50, "aa_cov": 0.7704918032786885, "hmm_cov": 0.96, "evalue": 5.331724037261905e-10, "score": 35.3817253112793, "start": 4, "stop": 50 } ], "seq_stats": { "molecular_weight": 7176.442300000001, "isoelectric_point": 8.597842216491703 }, "id": "GLJLFKCOHC_63", "locus": "GCA013368035_00021" }, { "type": "cds", "sequence": "contig_1", "start": 10330, "stop": 10818, "strand": "-", "frame": 2, "gene": "mccF", "product": "Microcin C7 resistance MccF related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "nt": "ATGATCAAACCAATAAAATTAAGTAAAGAAAATGGCATTGCAATAGTAAGCTTATCTGCAGGAACTATTGGAGAAGAATTTGCTAGTCACCAATTAGATTTAGCAATAAAAAGACTCAATTTGGATTTTCAACTTAACGTTATGATTGCTAAAAACGCACTTAAAGGAATAAAAGTTTTAAAAGAAAATCCTATGTTAAAAGTTGAGGATTTAAATGAAGTTTTTAGCAATGTCAATATAAAAGCAATAATTTGTGCAATTGGTGGATTTGACACGTACAAAACTATTCCACTTTTATTAGAAGATGAAAACTTTAAAAATTTAGTCTTAAATAATCCTAAAATTTTTATTGGATATTCAGATTCAACCATTAACCATTTAATGTTTTATAGATTAGGACTAAAAACATTTTATGGGCACAACATTTTATGCGATATTGCTGAACTTGACAAAGAAATGTTGCCTTATACTAAAAACTCATTTAAATGA", "aa": "MIKPIKLSKENGIAIVSLSAGTIGEEFASHQLDLAIKRLNLDFQLNVMIAKNALKGIKVLKENPMLKVEDLNEVFSNVNIKAIICAIGGFDTYKTIPLLLEDENFKNLVLNNPKIFIGYSDSTINHLMFYRLGLKTFYGHNILCDIAELDKEMLPYTKNSFK", "aa_hexdigest": "b2122f356082fbd59cdc6becf04b7887", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00308136E8", "query_cov": 0.9938271604938271, "subject_cov": 1.00625, "identity": 0.565, "score": 185.0, "evalue": 1.97e-57, "valid": false, "product": "LD-carboxypeptidase", "uniref50_id": "UniRef50_X0PHG8" }, "pscc": { "uniref50_id": "UniRef50_X0PHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X0PHG8" ], "product": "Microcin C7 resistance MccF related protein" }, "genes": [ "mccF" ], "id": "GLJLFKCOHC_64", "locus": "GCA013368035_00022" }, { "type": "cds", "sequence": "contig_1", "start": 11818, "stop": 12219, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTGCAACTTAGAAAAGTGTCATCAAGTAGTTTGTCTATTCAAACAATAGGAAGAATTAAAAGATTCCCAAATCCAAGTTATAACAAAGATCAAATTTCTAGAGAAGGCAATAGATACTATATTTATTCAAATGCTCAAACCAAAGAAAATATAAGAAGAACATTGATTTTAAAACGAGAATTTGAAAATGAAAAATTTGTTTATGGCCAAATAAATAAAGTAAAAATCAAACAAGCAATAATAGGAGCAGATTTTATTAGAGAAGCTAAAAATTCTATTGTTAAAGAAAAAATTGATGAATATTTTAAAAAATACCATAAAGAATATATTAAAAACAACAAGAAATTAATAGAAGAACAAGGAAACATTGAAAATAAAATTAGAGTTTATTCATGA", "aa": "MLVQLRKVSSSSLSIQTIGRIKRFPNPSYNKDQISREGNRYYIYSNAQTKENIRRTLILKREFENEKFVYGQINKVKIKQAIIGADFIREAKNSIVKEKIDEYFKKYHKEYIKNNKKLIEEQGNIENKIRVYS", "aa_hexdigest": "a2a73c52ca3c5a9f8a6ba8e9f05905ef", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15897.126499999988, "isoelectric_point": 9.932660102844238 }, "id": "GLJLFKCOHC_65", "locus": "GCA013368035_00023" }, { "type": "cds", "sequence": "contig_1", "start": 12238, "stop": 13362, "strand": "-", "frame": 2, "gene": null, "product": "DEAD/DEAH box helicase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "nt": "ATGAATCTAACTAATGTACAAGAAAAAGCAGTTAAACAATTGCTTGAAGAATATAAAATTGCAATTCAATCTAGTGAACCTAAAATAGTTGATTTTCAAGCTCCAACTGGTTCTGGGAAAACATTTATGTTAGCTAATACTATAAATGAAATAATAAAAGAAAATAATGCCAATGGTGAACCAAACAAGTTAGTCTTTGTTATTGCCACACTTTCATCGGCAGATTTACCTTATCAAATGGAATCAAACCTAAATGAATATAAAAGATATATTAATGGTTTATATGAAGTAGAAAGAAAGGAATCTCCTTCCTCAAATTCTCAAAAAAATAAAGATTCAACATATAATTTAATTCCTCAAAAAAATAAAGTAATAATCTTCGGTGTTTCATCGTTTGGTAAAAATAGAATATTTACCGAACAAGGTTTGCTTGAAAGCTTTCTTGATCAAATTAAATATCAAAACTATACATTAGTTTATATTAGAGATGAAGCGCATCATGGTGGAAATATAAAAGGTAGAAGTAATTTCAAATTTGAAGATATTGATACAATCCATGATGAATCTAAATTAAAAAAAGAAGAAGTTAGATTTGAATATAAAATTCAAAAAGCAGCCAATTTAATAATAAAAATGACAGCAACTCCTAAAGGAACACATAAACTTGTCTATATTGATGAAAAGGATTTAGCCGAAGATGATATTAAATTGCTAAAAGATGAATTAATTTATAACAAGGGTATAACTGACTCAAGTCAAGATTTAATTGATGATAATGAATTACTAAATCAAGCATGTGAAACATTTAAAGAAGTTCAAAGCCAATATGCAAACAATAATAGTGATTTAAAAAATATAAGACCAGCAATGCTTATTCAAGTTGAAAATGAACCAAGTGAAAAATCAACTGCCAAAGAAAAAGATGATTTTAAAGAAAGCATTGAAAACATAATTAAAAAATTAAATGAATGTAATTTGAGTTATGTTAAATACTTTTCAAATGATAAAGTGGAATCTTCTTCTTTAATAAAGGAAGAAAATCACAAAATAAACTTAAAAGAAATTTCAAAAAATGACTCTAATGTTGATGCAATAATTTTTAAAATTGGTCCTTCAACTGGTTGA", "aa": "MNLTNVQEKAVKQLLEEYKIAIQSSEPKIVDFQAPTGSGKTFMLANTINEIIKENNANGEPNKLVFVIATLSSADLPYQMESNLNEYKRYINGLYEVERKESPSSNSQKNKDSTYNLIPQKNKVIIFGVSSFGKNRIFTEQGLLESFLDQIKYQNYTLVYIRDEAHHGGNIKGRSNFKFEDIDTIHDESKLKKEEVRFEYKIQKAANLIIKMTATPKGTHKLVYIDEKDLAEDDIKLLKDELIYNKGITDSSQDLIDDNELLNQACETFKEVQSQYANNNSDLKNIRPAMLIQVENEPSEKSTAKEKDDFKESIENIIKKLNECNLSYVKYFSNDKVESSSLIKEENHKINLKEISKNDSNVDAIIFKIGPSTG", "aa_hexdigest": "817029b2dae9ecb8f080c21750f06fba", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0001981E5C", "query_cov": 0.8903743315508021, "subject_cov": 1.021472392638037, "identity": 0.598, "score": 366.0, "evalue": 8.38e-123, "valid": false, "product": "DEAD/DEAH box helicase family protein", "uniref50_id": "UniRef50_UPI001E2BAF28" }, "pscc": { "uniref50_id": "UniRef50_UPI001E2BAF28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2BAF28" ], "product": "DEAD/DEAH box helicase family protein" }, "genes": [], "id": "GLJLFKCOHC_66", "locus": "GCA013368035_00024" }, { "type": "cds", "sequence": "contig_1", "start": 13349, "stop": 13462, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAATGATTTTGACTCATCGAATGTAAATAAAATTAACGAACAAGAAATATTACTAAATTTAACTGCTTTAAAACCAATTGAAGGAAAAGATAACAATGAATCTAACTAA", "aa": "MLNDFDSSNVNKINEQEILLNLTALKPIEGKDNNESN", "aa_hexdigest": "16016a7433588c60b88e2454c0d26669", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4175.542200000002, "isoelectric_point": 4.196388816833497 }, "id": "GLJLFKCOHC_67", "locus": "GCA013368035_00025" }, { "type": "cds", "sequence": "contig_1", "start": 13574, "stop": 14044, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01555.25" ], "nt": "ATGATTAAAAATGAAATAATTGTTGAAAATTCAAAGGGTATTTTGTATGTAAAAAATTATAAAAACCTAGAAATTAAAAAAGAAGGAAAAGAATACAAATTATGTAAAAAAGATGGAGGAATTGATTGGTCTTCTTCAAAATTAATGGATAATATCTATTCAAATTATTATGGAACATTAGATTTGAAACAAATTTTTAATTGCGATAAATTTTCATATCCAAAACCCAAAAATTTAATAAAACGAATAATATCTTCTTGTCCTAATCCTAATGCTCGTATTCTTGACTTTTTTGCAGGTTCAGGAACAACAGGTCATGCAGTTTTAGATTTAAATAAAGAAGATGGGGGTAATAGAACCTTCACATTAGTTACAAATAATGAAAATAATATTGGCATAAATGTTTGCTATGAAAGGCTTTATAGAATTAATAAAGGCAAGGGAAGTAAAGGTGAAACCGACTTTGATTGA", "aa": "MIKNEIIVENSKGILYVKNYKNLEIKKEGKEYKLCKKDGGIDWSSSKLMDNIYSNYYGTLDLKQIFNCDKFSYPKPKNLIKRIISSCPNPNARILDFFAGSGTTGHAVLDLNKEDGGNRTFTLVTNNENNIGINVCYERLYRINKGKGSKGETDFD", "aa_hexdigest": "24a1b6892b1656307b4e29ecffc1783a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01555.25", "name": "N6_N4_Mtase", "length": 125, "aa_cov": 0.7564102564102564, "hmm_cov": 0.848, "evalue": 5.039697527553751e-16, "score": 54.68551254272461, "start": 15, "stop": 132 } ], "seq_stats": { "molecular_weight": 17737.00339999999, "isoelectric_point": 9.045704078674316 }, "id": "GLJLFKCOHC_68", "locus": "GCA013368035_00026" }, { "type": "cds", "sequence": "contig_1", "start": 14210, "stop": 14482, "strand": "-", "frame": 1, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6E811" ], "nt": "ATGATGAACGAAAGATTAAAAATGGCTCGCCAACTTTTAAAAGAAGATGGCGTTATTTTTGTTTCAATTGATGACAATGAACAAGCATATTTAAAAGTATTAATGGACGAAATTTTTGGAGAAGAAAATTTTGTGGCATGTTTAGCAAGAATAACCAAAAAAGGTGGCGGAAGATTTGGAAATTCAAATATTCAAAAAGATACAGATTATATATTAGTTTATTGCAAAAATATTTATAAAGCCTTTAAATTTAAAAAAATAGAAAGTGATTGA", "aa": "MMNERLKMARQLLKEDGVIFVSIDDNEQAYLKVLMDEIFGEENFVACLARITKKGGGRFGNSNIQKDTDYILVYCKNIYKAFKFKKIESD", "aa_hexdigest": "2c110c3f639fc5568795caa10ee28f93", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0035C6E811", "query_cov": 0.8111111111111111, "subject_cov": 0.9605263157894737, "identity": 0.562, "score": 80.1, "evalue": 4.71e-18, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0035C6E811" }, "pscc": { "uniref50_id": "UniRef50_UPI0035C6E811", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035C6E811" ], "product": "DNA methyltransferase" }, "genes": [], "id": "GLJLFKCOHC_69", "locus": "GCA013368035_00027" }, { "type": "cds", "sequence": "contig_1", "start": 14489, "stop": 14983, "strand": "-", "frame": 1, "gene": "rsmD", "product": "RsmD family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CF3C247" ], "nt": "ATGAAGGATTTAAAAAACTATATAGATGATATAGATAACTTGAATTCTTTTTTATTAAATCAAGACCAAAAGAAATTAATAAAAGAAATTTTAGTTAACACAAACAATTCAAATAAAGAAAACTTGGACAATGTTTTTCAACTTTTGATTCAAAGAGTTAAAACGGGCTTTGTTTTTGATGCGGCGCCTAGTGTTGATACAAAACAAATTGCAATATTAAAAAAACAAGGAAAATTATCATTTACAAATGATTTATTGAATACTAATAAAAACACTTTAATTATTGGCGAAAATTATGATGCATTAAAAAACCTACTTGTAATAGAAAGACAAAAAGAACAAATAGGGGGGGGGGATTATAATTACGATTTAATATATATTGATCCTCCTTATAATACTGAAAGTGCAAAAGATGATGGCAACAATCTTTCTGAAAAAGATGATGTTGAGGCATCTAAATTTATTTATAGAGACAAATTTAGTCGCACAGGTTGA", "aa": "MKDLKNYIDDIDNLNSFLLNQDQKKLIKEILVNTNNSNKENLDNVFQLLIQRVKTGFVFDAAPSVDTKQIAILKKQGKLSFTNDLLNTNKNTLIIGENYDALKNLLVIERQKEQIGGGDYNYDLIYIDPPYNTESAKDDGNNLSEKDDVEASKFIYRDKFSRTG", "aa_hexdigest": "72bfa69430d571335cb01f24815ee870", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00046621B4", "query_cov": 1.024390243902439, "subject_cov": 0.9081081081081082, "identity": 0.5539999999999999, "score": 159.0, "evalue": 6.86e-47, "valid": false, "product": "RsmD family RNA methyltransferase", "uniref50_id": "UniRef50_UPI001CF3C247" }, "pscc": { "uniref50_id": "UniRef50_UPI001CF3C247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CF3C247" ], "product": "RsmD family RNA methyltransferase" }, "genes": [ "rsmD" ], "id": "GLJLFKCOHC_70", "locus": "GCA013368035_00028" }, { "type": "cds", "sequence": "contig_1", "start": 15291, "stop": 15380, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGTCAAACACCAATAAAAAAAGCAATTTGA", "aa": "MKNKEIDAKQAKQERAEKLFGQTPIKKAI", "aa_hexdigest": "64c0078cc5b697f9a98ccb8247f59a16", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3342.9089000000004, "isoelectric_point": 9.873542594909669 }, "id": "GLJLFKCOHC_71", "locus": "GCA013368035_00029" }, { "type": "cds", "sequence": "contig_1", "start": 15414, "stop": 15779, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTGGACTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTACATTCAATTACGAAATTAGCCGAAATTACTCCTAATAGTATTGTTTCAACGGCGTCGGCAGTATTTGGCCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTACCTGTTGGAGCTTCAATTTATTACACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACAATGTTTTGA", "aa": "MAGLYGFIDQIFILNLVPKNRNIFGNNESEIVQFLNPALHSGGFFSDYKIMIEQYNLHSITKLAEITPNSIVSTASAVFGPLILFSNAIVFLVPVGASIYYTKCVSKQLKRAGQDLWATMF", "aa_hexdigest": "4beee402e996a0841af802bd2b8d8f2a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13453.499499999984, "isoelectric_point": 7.86406307220459 }, "id": "GLJLFKCOHC_72", "locus": "GCA013368035_00030" }, { "type": "cds", "sequence": "contig_1", "start": 16674, "stop": 16826, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGTTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTCGCCTCAATATTTGAAAACTTTATTTGCTTTGTAATAATTGTTCCTATTGGATATGGAATAGCTTGA", "aa": "MSLRIIAVSFVVVSLLAFQGSNDIAKSIFASIFENFICFVIIVPIGYGIA", "aa_hexdigest": "7e3886a0f6f4f97d10d162cf23a13065", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5395.421199999997, "isoelectric_point": 5.820676612854004 }, "id": "GLJLFKCOHC_73", "locus": "GCA013368035_00031" }, { "type": "cds", "sequence": "contig_1", "start": 17179, "stop": 17640, "strand": "-", "frame": 2, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "RefSeq:WP_047207547.1", "SO:0001217", "UniParc:UPI000640192E", "UniRef:UniRef100_UPI000640192E", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_D1J7F6" ], "nt": "ATGGAAAATAATTCAAAAAACTTGAAAACCAAAGAAAATGCAAAACTTCTAATAATATTTTCAGCAATCTTATTAGGAATAGATGTTATTTATATTATTTGTGGTTCGCTTGTACCAGTATTTGGATTATTATTAGCAATAGCAAGCATCGTCTTTTTTATTCTAACAGTTGTTTATGGATTTAAAACAGGATCTCGCGGCTTAAGTAAAAATGAAATAACAACAAGAAAATTATCAATTTCGTTAGTTGCCTTGTTTATAATTTCATTCGTCTTAGCAATTGTTGCTACAGCAATGTTTTTATCAGGCGCTGCTAAAACTGCTATAGCAATATATGGACTTTCAGTAATTGCTAATCTTGCACAATTAGGTGTTTTAATTGCATTATTATGTTTTGCAGTAAAAACTTATAATAGTAAAAACAATTCACACCCAGCCCAACAACAAAATTTAAGACAATAA", "aa": "MENNSKNLKTKENAKLLIIFSAILLGIDVIYIICGSLVPVFGLLLAIASIVFFILTVVYGFKTGSRGLSKNEITTRKLSISLVALFIISFVLAIVATAMFLSGAAKTAIAIYGLSVIANLAQLGVLIALLCFAVKTYNSKNNSHPAQQQNLRQ", "aa_hexdigest": "14444f6586ee2263f6b38f2740978bc1", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000640192E", "ncbi_nrp_id": "WP_047207547.1", "uniref100_id": "UniRef100_UPI000640192E", "db_xrefs": [ "SO:0001217", "UniParc:UPI000640192E", "RefSeq:WP_047207547.1", "UniRef:UniRef100_UPI000640192E" ] }, "ips": { "uniref100_id": "UniRef100_UPI000640192E", "uniref90_id": "UniRef90_D1J7F6" }, "psc": { "uniref90_id": "UniRef90_D1J7F6", "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_74", "locus": "GCA013368035_00032" }, { "type": "cds", "sequence": "contig_1", "start": 17822, "stop": 18355, "strand": "-", "frame": 1, "gene": null, "product": "DUF996 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1", "UniRef:UniRef90_A0A454C8X1" ], "nt": "GTGAATAATAACTTAAAAACTAAAGAAAATGCAAAACTTCTAATAATATTTTCATCAATCTTATTAGGAATAGATGTTATTTATATTATTAGTGGTTCGCTTGTGCCAATATTTGGATTATTATTAGCAATAGCAAGCATTGTGTTTTTTATTTTAACAGTTGTTTATGGATTTAAAACAGGATCACGTGAACCAAAAAATAGTAATCGAATGATAATAAGAAAATTATCCATTGCATTAATAGCATTGTTTGCAGCAGCAATTGCAATGGTTATTGTTGCAATAATAATAGCAATATCTTTATCACTTGGCGCATATAATTATGAATACCCAACAAATGAATATAATCCATTTTTATTAGAGTCATCAACATTTGCACTATATATTATTTGTTTAATTTTGATCCTTGCAGAATTAGGCGTTTTAATTGCATTATTATGTTTTGCAGTTAAGGTCTATTGCGCTAAAAATAACAATAATGTTAATAATGAAACAAATAACTATGATGGAAGCTCTCAAGGCCCCCAAAATTGA", "aa": "MNNNLKTKENAKLLIIFSSILLGIDVIYIISGSLVPIFGLLLAIASIVFFILTVVYGFKTGSREPKNSNRMIIRKLSIALIALFAAAIAMVIVAIIIAISLSLGAYNYEYPTNEYNPFLLESSTFALYIICLILILAELGVLIALLCFAVKVYCAKNNNNVNNETNNYDGSSQGPQN", "aa_hexdigest": "f77eeb90fedf167478bd514dc3bc30d2", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A454C8X1", "query_cov": 0.9943502824858758, "subject_cov": 0.9411764705882353, "identity": 0.972, "score": 279.0, "evalue": 9.05e-94, "valid": true, "product": "DUF996 domain-containing protein", "uniref50_id": "UniRef50_A0A454C8X1" }, "pscc": { "uniref50_id": "UniRef50_A0A454C8X1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C8X1" ], "product": "DUF996 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_75", "locus": "GCA013368035_00033" }, { "type": "cds", "sequence": "contig_1", "start": 18481, "stop": 19365, "strand": "-", "frame": 2, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "TTGTCTGAAACATTAAAGAAGAGAGTGAAGGGCCAAGATCAAGCAATTGAACTAATCTCTGATGCAATCAAGCGTTCTAAAGCAAACATCAATGACCCAAATAGACCAATTGGTTCGTTCCTATTTTTAGGGCCAACTGGTGTTGGTAAAACTGAACTTGCAAAAAGTTTAGCAGAAGCTTTATTTGATGATGAAAATCACATTGTAAGATTAGACATGTCTGAATATATGGAAAAACACAGCGTGTCAAAATTAATTGGATCGCCTCCAGGTTATATTGGTTATGATAATGGGGGACAACTAACTGAAAAAATTAGACAAAATCCTTACTCAATAGTTTTATTTGATGAAATTGAAAAAGCAAATTCAGATGTTTTAAATATATTGTTACAAATATTGGATAATGGTACCTTAACAGATAGTACAGGAAGGGTAGTAAACTTTAAAAACACAATAATTATCATGACTTCAAACATTGGAAGTAGTGAAATTATAAATAAGAGTTTAACCATTGATGGGGTAAGGGCATTATTATTAAGAAGTTTTAGACCAGAATTTATTAATAGAATCGATGAAATAGTGCCATTCAACCCATTAAGTCCTGCCGTTGTTTGCGATATTGTAAAACTAGAATTATCAAAATTAAAGAAAAGAGTTATTAATAACCATAATTTAGTAATTAATTTTGATGACAAAATAGTTAAATTCGTTGCTGATAACGCTTACGATTCCGCTTTTGGCGCTCGTCCAATTAAAAGATATATTCAAAAAAACATTGAAAACAAATTAGCAATGGAAATCATAAAAGGAAATTTAGTTGAAGGTGACGTTGCCTTATTAACAGTTTCAAATAATAATGTTATTGAATTACAAACAAATAATTAA", "aa": "MSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN", "aa_hexdigest": "28a3a307c9f5b3ecd2df9033bc508995", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_U4KPN6", "query_cov": 0.9829931972789115, "subject_cov": 0.8376811594202899, "identity": 0.5539999999999999, "score": 315.0, "evalue": 1.22e-103, "valid": false, "product": "ATP-dependent Clp protease ATP-binding subunit, 2", "uniref50_id": "UniRef50_A0A356L436" }, "genes": [ "clpA", "clpB" ], "truncated": "3-prime", "pseudogene": { "start": 18481, "stop": 19665, "observations": { "mutation": [ 19422 ], "stop": [ 19422 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.4106145251396648, "identity": 0.997, "score": 563.0, "evalue": 2.76e-196, "gene_start": 1, "gene_end": 294, "reference_start": 423, "reference_end": 716, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.9974619289340102, "score": 754.0, "evalue": 1.2e-273, "description": "Internal stop codon at 19422. Nonsense mutation around 19422.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "GLJLFKCOHC_76", "locus": "GCA013368035_00034" }, { "type": "cds", "sequence": "contig_1", "start": 19420, "stop": 20631, "strand": "-", "frame": 2, "gene": "clpB", "product": "Chaperone ClpB", "db_xrefs": [ "COG:COG0542", "COG:O", "SO:0001217", "UniRef:UniRef50_I5D648", "UniRef:UniRef90_D1J7F8" ], "nt": "ATGGAAGCAAGTTATACACCTTATGAACAAGAAAAAGATCCCTTGAAAGAATTTGGTCGAAACTTAACTGAGTTAGCTACGCAGAACAAACTAGAACCTGTTATAAACCGTGATGAAGAAATAAGATCACTAGTTAGAATTTTGTCAAGAAAGACTAAAAATAATCCTGTTTTAGTTGGTGAACCTGGTGTAGGCAAAACAGCAATTGTTGAAGGCCTAGCAAGGAAAATTGTTGAAAATCAAGTTCCAGAAAACCTAAAAGGTAAACAATTAATTGAAATTGATTTGCCTGCATTGGTTGCAGGAACACAATATCGTGGTCAATTTGAAGAAAGACTAAAAAAAGTATTGAAAAAGATTGATGAAGCAAATGGTGAAATCATTCTTTTCATTGATGAAATTCATATGATAATTGGCGCCGGAGGAACTGGCGATAGCAATATGGATGCGGCGAATATATTGAAACCTATGATGGCAAGAGGACAATTGCATCTAATTGGGGCAACAACATTGGATGAATATAGAAAATATATTGAAAAAGATCCAGCTCTTGAAAGAAGAATGCAAAAGATTTTAATTTCAGAACCTTCAATTGAAGATACCATAACTATATTGCGTGGTATAAAAGAAAGATTTGAAGCATATCATGAAGTTAAAATTGATGACTCTGCTATAGTTTCGGCAGCAAATTTATCAGCAAGATATATTGCGGATAGATTTTTGCCCGATAAAGCAATAGATTTAATTGATGAAGCAGCATCAAATATAAAAACTGAAATGAACTATTTGCCTGAACCTCTAGAAAAAATTAATTATCAAATTGCTAGATTAGAAATTGAAAAAGCTGCTCTAACAAATGATAGTGAAACAAAACAAACAGCTATTAAAAATAAAGAAAGAATTTTAGAAATAGATAAAGAACTAAATACCTTAAAATTGCAAAAAGTTGACCTTGAAAAACGCTGGAAGCAAGAAAAAGAAGATATTCAAAAATTTTCTTCAATTAAAGAAAACATTGAAGAGTTGAATAGAAAATTGCCTTTACTACAAAGTGAAGGAAAATATGTTGAAGCTTCTAAAATTATGTATGTTTTAGTTCCTGAATTAATTAAAACTAGAGATGAATTGGAACAAAAAATACAATCAAGGAAAAATCGTTTAATCAAAGAAGTTGTTGATGCTGAAGAAGTTGCAGATATTGTTTCAAAATGA", "aa": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSK", "aa_hexdigest": "e7cfa570cca2fe16147fef4315f4855e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001C99829F", "query_cov": 0.8933002481389578, "subject_cov": 0.975609756097561, "identity": 0.531, "score": 370.0, "evalue": 1.55e-123, "valid": false, "product": "AAA family ATPase", "uniref50_id": "UniRef50_A0AA38HIJ9" }, "genes": [ "clpA", "clpB" ], "truncated": "5-prime", "pseudogene": { "start": 19117, "stop": 20631, "observations": { "mutation": [ 19422 ], "stop": [ 19422 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F8", "query_cov": 1.0, "subject_cov": 0.5628491620111732, "identity": 1.0, "score": 753.0, "evalue": 3.45e-269, "gene_start": 1, "gene_end": 403, "reference_start": 1, "reference_end": 403, "reference_sequence": "MEASYTPYEQEKDPLKEFGRNLTELATQNKLEPVINRDEEIRSLVRILSRKTKNNPVLVGEPGVGKTAIVEGLARKIVENQVPENLKGKQLIEIDLPALVAGTQYRGQFEERLKKVLKKIDEANGEIILFIDEIHMIIGAGGTGDSNMDAANILKPMMARGQLHLIGATTLDEYRKYIEKDPALERRMQKILISEPSIEDTITILRGIKERFEAYHEVKIDDSAIVSAANLSARYIADRFLPDKAIDLIDEAASNIKTEMNYLPEPLEKINYQIARLEIEKAALTNDSETKQTAIKNKERILEIDKELNTLKLQKVDLEKRWKQEKEDIQKFSSIKENIEELNRKLPLLQSEGKYVEASKIMYVLVPELIKTRDELEQKIQSRKNRLIKEVVDAEEVADIVSKWTNIPVNRLLENQKQKILNLSETLKKRVKGQDQAIELISDAIKRSKANINDPNRPIGSFLFLGPTGVGKTELAKSLAEALFDDENHIVRLDMSEYMEKHSVSKLIGSPPGYIGYDNGGQLTEKIRQNPYSIVLFDEIEKANSDVLNILLQILDNGTLTDSTGRVVNFKNTIIIMTSNIGSSEIINKSLTIDGVRALLLRSFRPEFINRIDEIVPFNPLSPAVVCDIVKLELSKLKKRVINNHNLVINFDDKIVKFVADNAYDSAFGARPIKRYIQKNIENKLAMEIIKGNLVEGDVALLTVSNNNVIELQTNN" }, "paralog": false, "identity": 0.998015873015873, "score": 964.0, "evalue": 0.0, "description": "Internal stop codon at 19422. Nonsense mutation around 19422.", "psc": { "uniref90_id": "UniRef90_D1J7F8", "gene": "ClpA", "product": "Chaperone ClpB", "uniref50_id": "UniRef50_I5D648", "cog_id": "COG0542", "cog_category": "O", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F8", "UniRef:UniRef50_I5D648", "COG:COG0542", "COG:O" ] }, "pscc": { "uniref50_id": "UniRef50_I5D648", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D648" ], "product": "ATP-dependent Clp protease ATP-binding subunit" } }, "id": "GLJLFKCOHC_77", "locus": "GCA013368035_00035" }, { "type": "cds", "sequence": "contig_1", "start": 21309, "stop": 21542, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATTTATTGCCATGGGTTTCAGAAATAAATTCATCACTGTTTTTTATTATACTTTCAACCATTAATACAACTGTATTAGGTGCTTTATCAACTTTTTGTCTTTTTATTGCTACAAAAATTAGTATTGAAATAATAAAAATTAAAGCAACTAAAATAATTAAAGTAAATAATTGGTTGTCATTGAAACTGCTTACTTCGCCAGGATTAACTGGACCACCAAATCAATGA", "aa": "MSNLLPWVSEINSSLFFIILSTINTTVLGALSTFCLFIATKISIEIIKIKATKIIKVNNWLSLKLLTSPGLTGPPNQ", "aa_hexdigest": "3a7ae195b9c7dd9ef93ba2e382f97518", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8432.02649999999, "isoelectric_point": 9.748022651672361 }, "id": "GLJLFKCOHC_78", "locus": "GCA013368035_00036" }, { "type": "cds", "sequence": "contig_1", "start": 21552, "stop": 21959, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACTATCAAAAATATCAAAATATAAATTAAATGCTGGCAAGATCAATAACCCAATAGGGTTTAACTCAGCTGAAGGTGATTCTAAGTTAGCGCCAATAATTTTGGTTCAAATATTATTTAAGAATAAACTTACTAGTGCAACCAATACAACTCCTCCAAGTATGTTTCCATACATACGGAAAGTTAAACTTATTAATGGTGAAAAGTTTGATGATGCTTCTAAGGGATTAAGAATTTTAAATAAATACTTAAATTTTTTATATATGAATCCAATGATGTGTGTTCCAAGTCAACTTATAACAGTGGCAGCAAAAATTGCTGACAAACTAGTTCCAATTGGGGCTAAACCAACTAATGAAAGCATATTTCCTAAAATCAAGAAGGCAAATAATGAAATAAAATAA", "aa": "MKLSKISKYKLNAGKINNPIGFNSAEGDSKLAPIILVQILFKNKLTSATNTTPPSMFPYIRKVKLINGEKFDDASKGLRILNKYLNFLYMNPMMCVPSQLITVAAKIADKLVPIGAKPTNESIFPKIKKANNEIK", "aa_hexdigest": "cd316ae9f96adac2d24ff4871f142a36", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14983.714699999984, "isoelectric_point": 10.005122566223147 }, "id": "GLJLFKCOHC_79", "locus": "GCA013368035_00037" }, { "type": "cds", "sequence": "contig_1", "start": 22157, "stop": 22462, "strand": "+", "frame": 2, "gene": "atpE", "product": "ATP synthase F0 subunit C", "db_xrefs": [ "COG:C", "COG:COG0636", "EC:7.1.2.2", "GO:0015078", "GO:0015986", "GO:0045263", "RefSeq:WP_175933360.1", "SO:0001217", "UniParc:UPI00158D3D58", "UniRef:UniRef100_UPI00158D3D58", "UniRef:UniRef50_B3PLV3", "UniRef:UniRef90_D1J7G1" ], "nt": "ATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTGAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTATAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAA", "aa": "MDKLENIVNAFNTASAGVNSKEAGVNYPLAYGLTMLGAGIAVLGSGLVSIGQGIAVAKACEAVGRNPEAFSKIRTLLILGLGIVETGSIYCLVISLLLIFV", "aa_hexdigest": "c879cecb8f47ebfc100ac9fe218b5340", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D3D58", "ncbi_nrp_id": "WP_175933360.1", "uniref100_id": "UniRef100_UPI00158D3D58", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D3D58", "RefSeq:WP_175933360.1", "UniRef:UniRef100_UPI00158D3D58" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D3D58", "uniref90_id": "UniRef90_D1J7G1" }, "psc": { "uniref90_id": "UniRef90_D1J7G1", "gene": "atpE", "product": "ATP synthase F0 subunit C", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_B3PLV3", "cog_id": "COG0636", "cog_category": "C", "go_ids": [ "GO:0015078", "GO:0015986", "GO:0045263" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV3" ], "product": "ATP synthase subunit c" }, "genes": [ "atpE" ], "id": "GLJLFKCOHC_80", "locus": "GCA013368035_00038" }, { "type": "cds", "sequence": "contig_1", "start": 22467, "stop": 22592, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTCAATTGA", "aa": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFN", "aa_hexdigest": "14512e228ffe99de195f5f6ed3e87fe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4401.906899999999, "isoelectric_point": 8.254934883117677 }, "id": "GLJLFKCOHC_81", "locus": "GCA013368035_00039" }, { "type": "cds", "sequence": "contig_1", "start": 22635, "stop": 23048, "strand": "+", "frame": 3, "gene": "atpF", "product": "ATP synthase subunit b", "db_xrefs": [ "COG:C", "COG:COG0711", "SO:0001217", "UniRef:UniRef50_B3PLV4", "UniRef:UniRef90_D1J7G2" ], "nt": "TTGATTATTACTTTATTAGTATATAAACCAATAAAGAAATTAATTAAAAACCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAAGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCGGAAAGTGAAAGAATTGTCAACGCAAGCACGATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCGCAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAA", "aa": "MIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED", "aa_hexdigest": "be3df468688ee76264db8a0e355bc9c6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "atpF" ], "truncated": "5-prime", "pseudogene": { "start": 22467, "stop": 23048, "observations": { "mutation": [ 22590 ], "stop": [ 22590 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G2", "query_cov": 1.0, "subject_cov": 0.7098445595854922, "identity": 0.993, "score": 228.0, "evalue": 2.8e-74, "gene_start": 1, "gene_end": 137, "reference_start": 57, "reference_end": 193, "reference_sequence": "MFQNFANLLSQSQNAIAAAKPSASAKIAEELNKTFNGLTFNWPFFVFSLISLGLVVLIITLLVYKPIKKLIKNRQELIQKNIDTSILAKENALKVQEENDKKIIEANNQAALIINHAKSESERIVNASTIAAKKQSELLLDQAQILINKKQSELEQAQKKIIVENAVELAKKIIGREIRNEDNIKLINEVLED" }, "paralog": false, "identity": 0.9948186528497409, "score": 344.0, "evalue": 1.82e-122, "description": "Internal stop codon at 22590. Nonsense mutation around 22590.", "psc": { "uniref90_id": "UniRef90_D1J7G2", "gene": "AtpF", "product": "ATP synthase subunit b", "uniref50_id": "UniRef50_B3PLV4", "cog_id": "COG0711", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G2", "UniRef:UniRef50_B3PLV4", "COG:COG0711", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLV4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLV4" ], "product": "ATP synthase subunit b" } }, "id": "GLJLFKCOHC_82", "locus": "GCA013368035_00040" }, { "type": "cds", "sequence": "contig_1", "start": 23122, "stop": 23589, "strand": "+", "frame": 1, "gene": "atpH", "product": "F0F1 ATP synthase subunit delta", "db_xrefs": [ "COG:C", "COG:COG0712", "EC:7.1.2.2", "SO:0001217", "UniRef:UniRef50_D1J7G3", "UniRef:UniRef90_D1J7G3" ], "nt": "TTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTACTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCATTTTCAAAATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAAGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAA", "aa": "MALVSNEAAEITRLLKKNKDYLYVLNSYDIDENEKYRLIDEAFSKYNFYLVNTLKLAAKQHVIKYIIVILTKFVELSNEKRNIKYGFVYTISPLSERELKRLEVKLSNELKANIHLTNEIDPKLIAGIKIKVDDYLIDNSIVNKLNDFKKVVENN", "aa_hexdigest": "18d7ccde6f5b49b1e88707f70275fc6a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7G3", "query_cov": 1.0, "subject_cov": 0.8659217877094972, "identity": 0.9940000000000001, "score": 295.0, "evalue": 1.6e-100, "valid": true, "gene": "AtpH", "product": "F0F1 ATP synthase subunit delta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G3", "cog_id": "COG0712", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_D1J7G3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G3" ], "product": "ATP synthase subunit delta" }, "genes": [ "atpH" ], "id": "GLJLFKCOHC_83", "locus": "GCA013368035_00041" }, { "type": "cds", "sequence": "contig_1", "start": 23602, "stop": 24348, "strand": "+", "frame": 1, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "nt": "ATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATTGGGCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCTGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGTAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTAACCATTTATCTGGCCGTGTGTTAGATGCACTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAGATTGCACCTGGTGTTATGACTAGAAAAGAAGTTAATCAACCACTTGAAACCGGGATTTTAGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGTAAGACCGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAATGTTTATTGTATTTATGTTGCTGTTGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGA", "aa": "MSLEPNDLSAIIKAQIKHFNEDISKQEIGRVITVGDGIALVSGLSNVEYGELVKFESGVIGMALNLEEDLVGIVVMGDDRNIVENSTVYRTKQVISTPVGNHLSGRVLDALANPIDGKGPIQYEYTRPIFKIAPGVMTRKEVNQPLETGILAIDAMIPIGKGQRELIIGDRQTGKTAIAIDAILNQKGRNVYCIYVAVGQKNSTIAQIVDQLAKHDALKYTTIISASASDSAPLQYIAPYTGITIAEE", "aa_hexdigest": "9c6688dc667927208fb85f72a3d4e291", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A223M9E1", "query_cov": 0.9717741935483871, "subject_cov": 0.9450980392156862, "identity": 0.598, "score": 292.0, "evalue": 6.86e-97, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9E1" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9E1" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "GLJLFKCOHC_84", "locus": "GCA013368035_00042" }, { "type": "cds", "sequence": "contig_1", "start": 24349, "stop": 25050, "strand": "+", "frame": 1, "gene": null, "product": "ATP synthase subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "nt": "ATGGCAAGAGGTAAAGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCCGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCAATAACCGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGA", "aa": "MARGKDVLIIYDDLSKHAVAYRTLSLLLRRPPGREAYPGDVFYIHSQLLERAARVNSDYGGGSITALPIIETQAEDIAAYIPTNVISITDGQIFTKEALFNSGQRPAIDIGYSVSRVGSAAQTKLTKKVVSSLKLELAQYNEMLAFAQFGSDLDQATRTVLDHGAKVYELLKQPQYSPYSSIEQIIILFCTKYRLINVIPKEQILKYKTDILNYFRTNPNAINTLRAVSEAND", "aa_hexdigest": "b468ffcffd554434878d00167a9a7f5b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A223M9F0", "query_cov": 0.9141630901287554, "subject_cov": 0.841897233201581, "identity": 0.653, "score": 274.0, "evalue": 7.09e-90, "valid": false, "product": "ATP synthase subunit alpha", "uniref50_id": "UniRef50_A0A223M9F0" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9F0" ], "product": "ATP synthase subunit alpha" }, "genes": [], "id": "GLJLFKCOHC_85", "locus": "GCA013368035_00043" }, { "type": "cds", "sequence": "contig_1", "start": 25164, "stop": 25382, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00231.24" ], "nt": "ATGGAAAGTTTGCAAAAAATAAAACACAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTTGCAACTGCTAAGTTTGCTAAAATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACTTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGTGCTTCTTGA", "aa": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSAS", "aa_hexdigest": "04b8f1f836b6c54b2edfbab0ccaf8896", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00231.24", "name": "ATP-synt", "length": 64, "aa_cov": 0.8888888888888888, "hmm_cov": 1.0, "evalue": 1.7290341005741182e-18, "score": 62.676998138427734, "start": 3, "stop": 66 } ], "seq_stats": { "molecular_weight": 8315.497499999998, "isoelectric_point": 9.85201015472412 }, "id": "GLJLFKCOHC_86", "locus": "GCA013368035_00044" }, { "type": "cds", "sequence": "contig_1", "start": 25458, "stop": 26048, "strand": "+", "frame": 3, "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "db_xrefs": [ "COG:C", "COG:COG0224", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "KEGG:K02115", "SO:0001217", "UniRef:UniRef50_D1J7G5", "UniRef:UniRef90_D1J7G5" ], "nt": "ATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGTAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCTGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAACAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGTCAAAGAAGAACAGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATACAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAA", "aa": "MYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSTNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN", "aa_hexdigest": "67bdb602024a60fcd1860d1fa69632fe", "start_type": "ATG", "rbs_motif": null, "genes": [ "atpG" ], "truncated": "5-prime", "pseudogene": { "start": 25164, "stop": 26048, "observations": { "mutation": [ 25380 ], "stop": [ 25380 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G5", "query_cov": 1.0, "subject_cov": 0.6666666666666666, "identity": 1.0, "score": 368.0, "evalue": 3.7e-127, "gene_start": 1, "gene_end": 196, "reference_start": 99, "reference_end": 294, "reference_sequence": "MESLQKIKHRINSVNSTKKITKAMELVATAKFAKIKNQFENINNYYQSVERVFLNLIKNSDQNIDKLLNSASWKFKSKRNLYIVFGSDLGLCGPYNSEMYKKITQTVGKEDILIVVGSKLLSLIQKDNSHHIIQILTQIGDNPSYEIAQILSNKIYHVLEISWLKSIKLIYTQYINPIKSEPIVKEIFPISIDTLSTNNDNNLAGEESSKSIDPSCFEPNPTVILQNSFSIFFEASIYNALANAKLAEMSQRRTAMEQASDNAQDLINELNVQYNSSRQAKITQEITEIINGSN" }, "paralog": false, "identity": 0.9965986394557823, "score": 567.0, "evalue": 3.64e-208, "description": "Internal stop codon at 25380. Nonsense mutation around 25380.", "psc": { "uniref90_id": "UniRef90_D1J7G5", "gene": "atpG", "product": "ATP synthase F1 subunit gamma", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_D1J7G5", "cog_id": "COG0224", "cog_category": "C", "kegg_orthology_id": "K02115", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G5", "EC:7.1.2.2", "UniRef:UniRef50_D1J7G5", "COG:COG0224", "COG:C", "KEGG:K02115", "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7G5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G5" ], "product": "ATP synthase gamma chain" } }, "id": "GLJLFKCOHC_87", "locus": "GCA013368035_00045" }, { "type": "cds", "sequence": "contig_1", "start": 26065, "stop": 27552, "strand": "+", "frame": 1, "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "db_xrefs": [ "COG:C", "COG:COG0055", "EC:7.1.2.2", "GO:0015986", "GO:0045261", "GO:0046933", "RefSeq:WP_020002442.1", "SO:0001217", "UniParc:UPI00036A1184", "UniRef:UniRef100_A0A2K9YSL9", "UniRef:UniRef50_Q5FKY0", "UniRef:UniRef90_D1J7G6" ], "nt": "ATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGCGTTATTAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCTTTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGCGTGTTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATTGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTTGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGAGAAGGTAATGACCTTTATCACGAAATGCAAGCAAGTGGTGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTCAGATTTACACAAGCAGGTTCTGAAGTTTCTGCATTGTTAGGCCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACTTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTAGATCCAAATATAATTGGTCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGATGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGACGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAA", "aa": "MANKKTNENQEIQEEQNPVDESKNSENYGVISQILGSVIDVRFEEGHTPKILNALKIIDEDKKFSNEQILEVAQHVGNDTVRTIAMNLTIGLKRGMKVLDLGSPIKAPVGKEVLSRMFNVLGEPIDQKGGVFKHKMPIHRAAPSYEEQKSTLEIFETGIKVIDLLVPYIKGGKIGLFGGAGVGKTVLIQELINNIAKQHGGLSVFAGVGERTREGNDLYHEMQASGVLEKTALVFGQMNEPPGARMRAPFTALTIAEYFRDVMHQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLAVEMGQLQERITSTNNGSITSVQAVYVPADDLTDPAPATTFTHLDAKTVLDRDIAALGIYPAIDPLASSSRMVDPNIIGLEHYNTARAVQNILQRFKELQDIIAILGMHELSEEDKKVVARARRIRNFLSQPFFVAEKFSGIKGQYIKLSDTIRSFKEILSGKYDELPEEAFLYVGTIDDVIEKAKRLGVNVEK", "aa_hexdigest": "4a19b542e91cd0c2c735d0c3c4fad8a1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036A1184", "ncbi_nrp_id": "WP_020002442.1", "uniref100_id": "UniRef100_A0A2K9YSL9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036A1184", "RefSeq:WP_020002442.1", "UniRef:UniRef100_A0A2K9YSL9" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YSL9", "uniref90_id": "UniRef90_D1J7G6" }, "psc": { "uniref90_id": "UniRef90_D1J7G6", "gene": "atpD", "product": "F0F1 ATP synthase subunit beta", "ec_ids": [ "7.1.2.2" ], "uniref50_id": "UniRef50_Q5FKY0", "cog_id": "COG0055", "cog_category": "C", "go_ids": [ "GO:0015986", "GO:0045261", "GO:0046933" ] }, "pscc": { "uniref50_id": "UniRef50_Q5FKY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5FKY0" ], "product": "ATP synthase subunit beta" }, "genes": [ "atpD" ], "id": "GLJLFKCOHC_88", "locus": "GCA013368035_00046" }, { "type": "cds", "sequence": "contig_1", "start": 27552, "stop": 27929, "strand": "+", "frame": 3, "gene": "atpC", "product": "ATP synthase epsilon chain", "db_xrefs": [ "COG:C", "COG:COG0355", "RefSeq:WP_012855306.1", "SO:0001217", "UniParc:UPI0001BC1FD9", "UniRef:UniRef100_D1J7G7", "UniRef:UniRef50_A0A0C6FPC1", "UniRef:UniRef90_D1J7G7" ], "nt": "ATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGGCAGGCGCAACTGAGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCGCCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCTGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAA", "aa": "METPKIHIIISTPLGIYFDKEASICTFTTTEGQIGIMAGATEFMAALVPSKIMVNFLNSPDSKVFYIDKGIVQFKNNVLSIIVNQIDTKEIELDHKFSPEDQKKYTIIEEVTLKKSILKSQTNNK", "aa_hexdigest": "0a62504168c594b2b20b89e019f93a7b", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC1FD9", "ncbi_nrp_id": "WP_012855306.1", "uniref100_id": "UniRef100_D1J7G7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC1FD9", "RefSeq:WP_012855306.1", "UniRef:UniRef100_D1J7G7" ] }, "ips": { "uniref100_id": "UniRef100_D1J7G7", "uniref90_id": "UniRef90_D1J7G7" }, "psc": { "uniref90_id": "UniRef90_D1J7G7", "gene": "AtpC", "product": "ATP synthase epsilon chain", "uniref50_id": "UniRef50_A0A0C6FPC1", "cog_id": "COG0355", "cog_category": "C" }, "pscc": { "uniref50_id": "UniRef50_A0A0C6FPC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0C6FPC1" ], "product": "ATP synthase epsilon chain" }, "genes": [ "atpC" ], "id": "GLJLFKCOHC_89", "locus": "GCA013368035_00047" }, { "type": "cds", "sequence": "contig_1", "start": 28186, "stop": 28818, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0", "UniRef:UniRef90_UPI00215D6119" ], "nt": "ATGAAAAAAACAGCAAAAGCTTTATTAATAATATGCACAACTATAACACCTAGTTTTGCTATGCCATTAATATCGGCGGGATGTAATAAAACAACAAACCATGAAACAAACAAAGAAAATACAATTATAGATAAACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAACCCCAATAAGTTAGAATCCAACAGTGAATTAGCAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGACAAGATTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTTAATGATGGAAATAAAATCATTAAATTAGAAAATCAAAAATATTCAATGGCAGGACTTTCAACAAATAATGAAAGCTATTTAAATGGATTATTAAATGATAATGACACAATATTTAATTTAACAACTAATTTCAAAAAAGGTAATGAAATAGATGCATATCATTTAGCTAATAGCCAAGGCTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGA", "aa": "MKKTAKALLIICTTITPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKKFKIEILKLEADKKNNLISISYNISFNDGNKIIKLENQKYSMAGLSTNNESYLNGLLNDNDTIFNLTTNFKKGNEIDAYHLANSQGSISLLASNKNVYVPKDLILEYT", "aa_hexdigest": "5e1d5a690453937063a0fe346af1590e", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28186, "stop": 29121, "observations": { "mutation": [ 28816 ], "stop": [ 28816 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D6119", "query_cov": 1.0, "subject_cov": 0.40540540540540543, "identity": 0.9670000000000001, "score": 382.0, "evalue": 5.15e-129, "gene_start": 1, "gene_end": 210, "reference_start": 1, "reference_end": 210, "reference_sequence": "MKKTAKALLIICTTITPSFAMPLISAGCNKTTNHETNKENTIIDKQAPIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKNFKIEILKLEADKKNNLISISYNISFNDGNKITKLENQKYSMTGLSTNNESYLNGLLNDNDTMFNLTTNLKKGSEIDAYHLANGQGSISLLASNKNVYVPKDLILEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFNEGDIEQTLRNNFIPKFENSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVAGFKGIEKVDDEQAKKIVDKLTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLNFVEYFTKNGINNFNKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKN" }, "paralog": false, "identity": 0.9614147909967846, "score": 590.0, "evalue": 2.22e-213, "description": "Internal stop codon at 28816. Nonsense mutation around 28816.", "psc": { "uniref90_id": "UniRef90_UPI00215D6119", "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D6119", "UniRef:UniRef50_UPI00215CD1C0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215CD1C0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215CD1C0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23618.548000000017, "isoelectric_point": 8.629689598083495 }, "id": "GLJLFKCOHC_90", "locus": "GCA013368035_00048" }, { "type": "cds", "sequence": "contig_1", "start": 29299, "stop": 30249, "strand": "+", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G9", "UniRef:UniRef90_D1J7G8" ], "nt": "TTGACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAGTCAAAGTCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAATTAGAAAAACCTTTAAATTTTGCAGAATATTTTGCCAAAAACGGAATTAACAATTTTGATAAAAATTATATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAACGAAGTAGGTAAGGAATTTTTAGGAAATCCAAATGAAATTTTGGCAAGTGATTTCTCAGCTACAAATTTAAAATTGAATAAAAATGTTGGCGATAGTCTTGATAGTTTAAATTTTATTAAATTATCTTCAATAAAAAGACCTTCAAAAAATTCAGAGGAATTAAAGCTCAACGAAGAAGGTAAGGCAACGTTAATTTATGAATTTAGAAATGTATATGATAATTCAATATATACAACAGAAGTCGTATTTGAAAATCTAAAAAAAGTAGAAAAAGCTGATTATAAAAAACAACACGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGTGGTTGGGCAACTGCCGAAGAAATAAATGAATTGGAAACTTTTTTGCCAAGTCAAAAAAAATCTAAAAAAAATAAGAGAATAAACATTGAACATTATATAAACAATGGCAATATAGAAATACTTGTTGGAAGAGTAATTACTAAAAAAAATAAAATTACTTTCGGCGATACATTGAACAGACAAAAGGAATTTGAAGAAATAATATTTGATGAAGAATTTGTTGAAGCAGTTAAGAAATCATCTAATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGAAGAAAATTCTAAACATAAAAATCAAAAGAATTGA", "aa": "MTFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLEKPLNFAEYFAKNGINNFDKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPNEILASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNVYDNSIYTTEVVFENLKKVEKADYKKQHASLIQKINDKKIIKSGWATAEEINELETFLPSQKKSKKNKRINIEHYINNGNIEILVGRVITKKNKITFGDTLNRQKEFEEIIFDEEFVEAVKKSSNNKSENKILSNVITEENSKHKNQKN", "aa_hexdigest": "d89511a88b66307aa1d3e3904d3b8655", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 28999, "stop": 30345, "observations": { "mutation": [ 30247, 30277 ], "stop": [ 30247, 30277 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7G8", "query_cov": 1.0, "subject_cov": 0.43646408839779005, "identity": 0.968, "score": 578.0, "evalue": 1.07e-201, "gene_start": 1, "gene_end": 316, "reference_start": 377, "reference_end": 692, "reference_sequence": "MKKTAKALLIICTTITPSFAMPLISAGCNKTTNHETNKENTIIDKQATIKKQENDSNNPNKLESNSELAEAVKKANFQYKDIDKISMFDKLNKENLSNNLDKKFKIEILKLETDKKNNLISISYNISFNDGGKIIKLENQKYSLTGLSTNNESYLNGLLNDNDTIFNLTTNFKKGNEIDASQLDAYYLANSQGSISLLASNKNVYVPKDLILEYAWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSIDSFSEGDIEQTLKNNFIVKFEKSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVSGFKGVEKVSDEQVKKIVDKLAFGDVYFPEKVKTFSQSRLNKLNLYIKDENGKAQLENPLNFVEYFTKNGINNFDKNYIVKYDKAEKKLITTLKLNEKEYTIETKLTHESKPDEWVNEVGKEFLGNPKEISASDFSATNLKLNKNVGDSLDSLNFIKLSSIKRPSKNSEELKLNEEGKATLIYEFRNIYDNSIYTKEVVFENLKKVEKADYKKQYASLIQKINDKKIIKSGWATAEEINELETFLPSQKKSKKNKRINIEHYINNGNIEILVGRVITKKNKITFGDTLNRQKEFEEIIFDEEFVEAVKKSSNNKSENKILSNVITEENSKHKNQKNWIRLTKHEGKWCILFAFIEDTKSTPYYVQIEK" }, "paralog": false, "identity": 0.96875, "score": 852.0, "evalue": 6.85e-312, "description": "Internal stop codon at 30247, 30277. Nonsense mutation around 30247, 30277.", "psc": { "uniref90_id": "UniRef90_D1J7G8", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7G8", "UniRef:UniRef50_D1J7G9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7G9" ], "product": "Variable surface lipoprotein" } }, "id": "GLJLFKCOHC_91", "locus": "GCA013368035_00049" }, { "type": "cds", "sequence": "contig_1", "start": 30585, "stop": 31226, "strand": "+", "frame": 3, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF", "UniRef:UniRef90_UPI00215D5ADF" ], "nt": "ATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGAGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCCAAATAAACCAGAAGTTAAACCAGAGGATCCAAAGAAACCTGAAGGTGAAAAACCCGGAACAACTCCCGAAGTTAAGCCAGAATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGGTTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAACAACTTCGATAAAAAATTCAAAGTAGAAGTTTTAAATTTAGAAGTAGATAAGAAAATTAATTCAGTATTAATTACATACAACATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAACAATGAAAGCTATTTAAATGGATTATTGAACGATAAGGATACAGTATTCAATTTAGGTGATGCTTCTAATGATGATAGTTTAACAACAGTTGAACAGTTAGATGCATATTACTTAGTTGATGGTCGAGGTTCTATTAGTTTATTAGCATCTAATAAAAATGTTTATGTTCCTGAAGGTCTAATGTTAGAATATACTTGA", "aa": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPESNSDLNDLTEAVKKANFQYKDIDKVSMFDKLNKENLSNNFDKKFKVEVLNLEVDKKINSVLITYNISFNDGSKDAKLENQKYSLTGLSTNNESYLNGLLNDKDTVFNLGDASNDDSLTTVEQLDAYYLVDGRGSISLLASNKNVYVPEGLMLEYT", "aa_hexdigest": "868ec6c2131ae4233e77037a7f5ae5a4", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 30585, "stop": 31529, "observations": { "mutation": [ 31224 ], "stop": [ 31224 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D5ADF", "query_cov": 1.0892018779342723, "subject_cov": 0.5686274509803921, "identity": 0.884, "score": 369.0, "evalue": 2.46e-125, "gene_start": 1, "gene_end": 213, "reference_start": 1, "reference_end": 232, "reference_sequence": "MKKSKKILLTMGGILPLVISAPLVAAGCKEPNKPEVKPEDPKKPEGEKPGTTPEVKPEDPKKPEGEKPGTTPEVKPKSNSDLNDLTEAVKKANFQYKDIDKISMFDKLNKENLSNNFDKKFKVEVLNLEVDKKINSVLITYNISFNDGSKDAKLENQKYSLTGLSTNNESYLNGLLNDKDTVFNLGDASNDDSLTTVEQLDAYYLVDGLGSISLLASNKNVYVPEGLMLEYTWNEQNETNKKFNGEVELDVSVKTKDNKTITRKNVKIANKIAEGVSDDYLTKYKKLTLNYKDADKTSINSFKEGDIEQTLKNNFIAKFEKSSEEETKKFLEDNGIKYEVKSITEISLEKKTINVNLQITLKNKKLANANIEVSGFKGVEKVDDEQVKKIVDKLTFGDVYFPEKIKDF" }, "paralog": false, "identity": 0.9159159159159159, "score": 588.0, "evalue": 3.97e-214, "description": "Internal stop codon at 31224. Nonsense mutation around 31224.", "psc": { "uniref90_id": "UniRef90_UPI00215D5ADF", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D5ADF", "UniRef:UniRef50_UPI00215D5ADF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D5ADF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D5ADF" ], "product": "variable surface lipoprotein" } }, "id": "GLJLFKCOHC_92", "locus": "GCA013368035_00050" }, { "type": "cds", "sequence": "contig_1", "start": 31608, "stop": 32366, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATAAGAAACTTGCCAATGCAAATATTGAAGTTTCAGGATTTAAAGGCGTTGAAAAAGTAAGTGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAATAAAAGAAATTTCAAAAGATGAAACATTTTTGAATGATTTGCCTTTATATATCAAAGATGAACATCAAAAAACTAATCCCGAAAATTTTGTTAATTCTGAATATTTAATTAAAAATGGAATCACCGAATTTGATAAAAATGAATCTGGGCAACTTAACTACAAAGTCAAATATGATGAAAATGCAAAAAAATTGTCTGTAACATTAAAATTAAACGGCAAGGAATATAATGTTGAGACAACATTAACTCATGAATTAAAATTAGATGATTGGGTAAATGAAGTTGGAAAAGAAATTAATGGCGATTCAAATACCATTCCCGTAAATGAATTTTCTAATAAGAATTTAAAATTGAAAAAACAAATAAAAGAATTTGTTGCAGGTTTAGACTTTGTTCAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAGGCAGAAAAAAAATTGAATTTAGAAGGTAAAGCAACCTTAATTTATGAATTTAAAAATATATATGATAATTCAACATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAAGCAGAAAAAGTTGATTATAAAAAACAATATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGA", "aa": "MKNKKLANANIEVSGFKGVEKVSDEQVKKIVDKLTFGDVYFPEKIKEISKDETFLNDLPLYIKDEHQKTNPENFVNSEYLIKNGITEFDKNESGQLNYKVKYDENAKKLSVTLKLNGKEYNVETTLTHELKLDDWVNEVGKEINGDSNTIPVNEFSNKNLKLKKQIKEFVAGLDFVQLNSIKRAKSSEAEKKLNLEGKATLIYEFKNIYDNSTYTKEVVFENLKKAEKVDYKKQYASLIQKINDKKIITNNK", "aa_hexdigest": "1c6c4c3a8f041ce570abbe6066ff7b94", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29122.76820000003, "isoelectric_point": 8.764364051818848 }, "id": "GLJLFKCOHC_93", "locus": "GCA013368035_00051" }, { "type": "cds", "sequence": "contig_1", "start": 32508, "stop": 32636, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATTTTGGAGATAATGGTAAAGCTCGCGAATCATTTAAAGAGTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAAGGTTCTAAAAATAACCAATGA", "aa": "MHFGDNGKARESFKELKFDNDFLEAIKSKTLVNVVKGSKNNQ", "aa_hexdigest": "a145124e0ef8780d8256d6803baa72d8", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4785.352900000003, "isoelectric_point": 9.224345970153806 }, "id": "GLJLFKCOHC_94", "locus": "GCA013368035_00052" }, { "type": "cds", "sequence": "contig_1", "start": 32844, "stop": 33785, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1", "UniRef:UniRef50_UPI00215D9390", "UniRef:UniRef90_D1J7H1" ], "nt": "ATGACTAAGAAAAAAATATTATTTAAAATATTTGCTATGGGCACTATCCTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAGTGATACAGAAAATAAAGATAATAGTTTTGAAAATAATAGCAATAATGATCAAAGCAACCAACATGATTTAAGCACCGAAGATAATAATTCAAGAGATTTGATAGTTCCTGAATCAAATAATAAGCCCGAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTATCCCAACGCCTGAAATAACGCCCAAACCAGCGCCTGAAATAACGCCCGAACCAGCGCCTGAAATAACGCCCAAGCCAAATGAAAATGACTATAATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTACTAGAAAAAAAGGAGAATACATTGAACCAAAGCCATCGATTTTATTGGCTGATAGTTCAAATAGACAGTCAGATTTGAATAAACAAGGAGCCCAAGAAGTAAGTGAATTTCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCCAATTTTGTTATTTACGGTGGCGATACTAATATAAAAAATGAAAACTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAGACTTAAAATTAAAAAAATATGACCAAAAATTTCTAACATCGTTAGGAACAAGTGGAAAATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTCAAACCAAAAATAATTACCGATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAGCTTGAAAGCTTAAAAGGATGA", "aa": "MTKKKILFKIFAMGTILAGGVFSLNSCIFKSSDTENKDNSFENNSNNDQSNQHDLSTEDNNSRDLIVPESNNKPENDSIPQPESKPNIIPTPEITPKPAPEITPEPAPEITPKPNENDYNKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADSSNRQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRDLKLKKYDQKFLTSLGTSGKYSEQYDKMFFINQLPNTFKPKIITDGLLPYKVDIYRTFNDVISKAELESLKG", "aa_hexdigest": "76a8e0b0c2d932c4de2635e738a95434", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00215D9390", "query_cov": 0.8817891373801917, "subject_cov": 1.010989010989011, "identity": 0.855, "score": 409.0, "evalue": 9.28e-142, "valid": false, "uniref50_id": "UniRef50_UPI00215D9390" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9390", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9390" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 32844, "stop": 34088, "observations": { "mutation": [ 33783, 33954 ], "stop": [ 33783, 33954 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H1", "query_cov": 1.0511182108626198, "subject_cov": 0.6553784860557769, "identity": 0.809, "score": 458.0, "evalue": 1.28e-157, "gene_start": 1, "gene_end": 313, "reference_start": 1, "reference_end": 329, "reference_sequence": "MTKKKVLFKIFAMGAILAGGVFSLNSCIFKSNDTENKDNSFENNTNNGQRNQHDLSTEDNNSRDLIVPESNNKPENDSIPQPESKPNIIPTPEITPKPAPEITPEPTPKPAPEITPEPTPKPAPEITPKPNENDYNKANHNQTHFVRPPFIAKFKIKNTDFSIATIFNHLDSPGTRKKGEYIEPKPSILLADSSNRQSDLNKQGAQEVSEFLGLIDVINENKDLANFVIYGGDTNIKNENFYLARKYPENIQFTLDRDLKLKKYDQKFLTSLGTSGKYSEQYDKMFFINQLPNTFKPKIITNGLLPYKVDIYRTFNDVISKAELENLKGWKSKNNDKSTIRSQISDHAPVFTDVVFSNPIETLKVNESLRKNQTLSKEQNTIRIAHWNILNFGGPNQDAKTMAIATTIYKSGFDIIGLTEINDGRGNYVQNIVDLLNEYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN" }, "paralog": false, "identity": 0.8348837209302326, "score": 654.0, "evalue": 3.9e-237, "description": "Internal stop codon at 33783, 33954. Nonsense mutation around 33783, 33954.", "psc": { "uniref90_id": "UniRef90_D1J7H1", "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H1", "UniRef:UniRef50_D1J7H1" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H1" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35491.398700000005, "isoelectric_point": 5.731894111633301 }, "id": "GLJLFKCOHC_95", "locus": "GCA013368035_00053" }, { "type": "cds", "sequence": "contig_1", "start": 33999, "stop": 34304, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017", "UniRef:UniRef90_UPI00215DB2CF" ], "nt": "ATGGCTATTGCTACTACAATTTATAAGTCCGGTTTTGATATAATAGGCTTAACTGAAATTAATGATGGAAGAGGAAACTATGTGCAAAATATAGTTGATCTTTTAAATGAATATGCAGGAAAAGAAAGATATAAATTTGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCACAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCGAAGCTATTTAAAACCAATTAAGTATTACAAAGATAACTAA", "aa": "MAIATTIYKSGFDIIGLTEINDGRGNYVQNIVDLLNEYAGKERYKFVLQNKKDTNIRPEYLQSNKFGKAQQEQVAVIYDSQNFEFVSSRSYLKPIKYYKDN", "aa_hexdigest": "ac9b9d01e94377f5280df29b05d03fa3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00215DB2CF", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.96, "score": 193.0, "evalue": 1.65e-62, "valid": true, "uniref50_id": "UniRef50_A0A449B017" }, "pscc": { "uniref50_id": "UniRef50_A0A449B017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B017" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11760.098199999991, "isoelectric_point": 8.751792716979981 }, "id": "GLJLFKCOHC_96", "locus": "GCA013368035_00054" }, { "type": "cds", "sequence": "contig_1", "start": 34897, "stop": 35322, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATGCAATTGAAAAAATTAAATCAGCATTTAGCTTCAATGATGTTTTAGTATTATCTGAATTAGTAGGTGAAGATAAACCAATCAATGAAACTGCTTTGGTTAAATTAATCAATTTAGTATTTGGAAAAAGTAATTTTAATGACTCTGTAATCTATAATGCATTAAGAAATCTAAACAATAATTCAGATGAATCAAAACGTTCAAATATGAATACTTTAAATAGCTCGGTTTCAAATGCGTTGGGAAGCATTTTCAAACCTTCAAAAGCCAGCGATAAAGATGACCCCGATAATATTCAATATTCAGCAGACGTTTTAAAATTACTTGATACAATTTATAAATTGCTTGCAAAGGCATACAATAATGATAAGGATGCAAATTCATCAACATATAAAGTTAGGGCACAATCCGAAGCATGA", "aa": "MNDAIEKIKSAFSFNDVLVLSELVGEDKPINETALVKLINLVFGKSNFNDSVIYNALRNLNNNSDESKRSNMNTLNSSVSNALGSIFKPSKASDKDDPDNIQYSADVLKLLDTIYKLLAKAYNNDKDANSSTYKVRAQSEA", "aa_hexdigest": "574993f1b8913f1cb4eab2d1e512e246", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15572.18469999998, "isoelectric_point": 5.141393852233886 }, "id": "GLJLFKCOHC_97", "locus": "GCA013368035_00055" }, { "type": "cds", "sequence": "contig_1", "start": 35323, "stop": 35730, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTAATAAGTAAAAAATTCATTGATTTTCCAGAAATTAAAAGCTTAATTGAAGCCGTAGTTAATGATTTTCTAGCAGGAACAAAAATTGAATCGACCAACCTTGGGGACTTAATATCTAAAACAATTACTAGAATTCAAGATAATCTAATTAAAAACTCTTCTGAACTTATTAAGCATCTTGCAACTGATGATGAAATTACAAATCACATTGTTGAAAGTATTTTAAATTATCTAAAAATAGAAGGAAATGATGAAGATAAAAAATTCCTAAAAGACTTAATTGGAAAAATCGTACCAGCATTGTTAAATAATGATTTTATTAAAAGGAAATTCATTAAGCGTTCAATAGGATTGATAAGTAATTATGCTAAACAATTTGATATTTTAAATCCTGTTAAATGA", "aa": "MELISKKFIDFPEIKSLIEAVVNDFLAGTKIESTNLGDLISKTITRIQDNLIKNSSELIKHLATDDEITNHIVESILNYLKIEGNDEDKKFLKDLIGKIVPALLNNDFIKRKFIKRSIGLISNYAKQFDILNPVK", "aa_hexdigest": "081bf68c8d34d54d52557cf92b46b1c9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15371.71039999998, "isoelectric_point": 6.7399904251098635 }, "id": "GLJLFKCOHC_98", "locus": "GCA013368035_00056" }, { "type": "cds", "sequence": "contig_1", "start": 35767, "stop": 36456, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGCAAATAAAGATAAATTTAAAGCATTTATTAAAAACGCAGTAAATGAAATGACTTCTAACAAAAACTTAATTAACAAATCTATTGAATATGCATTGAATTTATTCAACACGAAATATTCAATTAGTTCAAGTAGCGCTCAAATTCAAGGAATTGCAAATCTTTTGACTAGATTAATTAACAAACTAGGTTCAATGAACGTATTTAGCAATATATTGGGAGATTTCATTGATTTATTTATTGATGTTGATTTCCTTGACAATAATGGTCATTTCAATGGAAATGCTTTGGCATCCAAACTTCTTGAATCTATTAAAAAGATTGAATACCATAACTATTTAACAACAGAAAATTTAAGTGAATTATTTGAAACAATATTAGATAAAAATATTGATAAAAATGTCTTGTATAATGAATTGAATTTCGCTTATTCATATATAGTTGACAATCTTGATAAATTTAAACAAGCATTCTCTGCTCAAAGTAATTCTACAACGTTAAACGAAGTAGATAAAGCTAAACAAGATAAAGAACACGAACAATTCCTAAAGAATTTAGAAAAATTAATTTACAATGCTTTAAAAGCTCTAAATGGATCAATAAAAGCAGATAATAAAAACGGAAAAGAAGCATTAGTTGATTTTGTATATAATGTTTTGAAGGATCAAATTAAGAAATTTAATTGA", "aa": "MSANKDKFKAFIKNAVNEMTSNKNLINKSIEYALNLFNTKYSISSSSAQIQGIANLLTRLINKLGSMNVFSNILGDFIDLFIDVDFLDNNGHFNGNALASKLLESIKKIEYHNYLTTENLSELFETILDKNIDKNVLYNELNFAYSYIVDNLDKFKQAFSAQSNSTTLNEVDKAKQDKEHEQFLKNLEKLIYNALKALNGSIKADNKNGKEALVDFVYNVLKDQIKKFN", "aa_hexdigest": "ff22ee915ac686aadf99326de1c88316", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26079.26210000003, "isoelectric_point": 6.844972038269043 }, "id": "GLJLFKCOHC_99", "locus": "GCA013368035_00057" }, { "type": "cds", "sequence": "contig_1", "start": 36496, "stop": 36786, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAATGCGTATTTTCAATATTAGATAATAAGGCAAAGAAAGAAATTAAAGATGAATTAAAAAATTCAGTTGATATTTTCTTGCATAGTAATAAATTCAAACAATATATCAAACACAATTTATTAAAAATAAAAGAACAAATTCTAAAAATTGATTCAAAAGCTTCTGAATTAGGAAATGAAATAGTTGAAACATTAATGAAGGTAATTTTTGATTCAAATAATACAAAAGAATTTATACTAACATTATTAAATTCATTGATAGATTTAGGCGATAACAATCAATGA", "aa": "MFKCVFSILDNKAKKEIKDELKNSVDIFLHSNKFKQYIKHNLLKIKEQILKIDSKASELGNEIVETLMKVIFDSNNTKEFILTLLNSLIDLGDNNQ", "aa_hexdigest": "2b0301264aaa1b867b6df8ee86554326", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11142.870299999988, "isoelectric_point": 7.816034126281738 }, "id": "GLJLFKCOHC_100", "locus": "GCA013368035_00058" }, { "type": "cds", "sequence": "contig_1", "start": 37157, "stop": 37372, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAGCGAAATTAATTTTAGTAATGTTTCTTTTGCATTAACAGGTCATGTAACATTCTTTAGATTTTGTTCCATCAATGAAATTAATAATTTTGCTATTGAATTTTTAATATCAATACCTAAGGTTTTGTTATTGATAAATTTATTGAATATTTCTTTTATTTTTTTAGTGACATATTCTTTATTATTTGAAATAAATTTGCTTATTCATTGA", "aa": "MPSEINFSNVSFALTGHVTFFRFCSINEINNFAIEFLISIPKVLLLINLLNISFIFLVTYSLLFEINLLIH", "aa_hexdigest": "c35c68038caf4c8682556442a5d729f3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8180.617899999992, "isoelectric_point": 5.385858345031737 }, "id": "GLJLFKCOHC_101", "locus": "GCA013368035_00059" }, { "type": "cds", "sequence": "contig_1", "start": 37366, "stop": 37560, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATACATTGATGAAATAAATATTTCAAGCTTTAAGGGTGATGTACAAGAAAACCTTCAACAAATAATTTCATATATAAAATCATTTGTTGAAACAAGTTACAATGAAGATCTTTTACCTTTAATAAAAGAGCTAATTAAAAAGTTATTTGATGATAATAATTTAAAAGATTTAAATAATATTTCTCAATGA", "aa": "MKYIDEINISSFKGDVQENLQQIISYIKSFVETSYNEDLLPLIKELIKKLFDDNNLKDLNNISQ", "aa_hexdigest": "ca8f3ab292e5527901cf62fcaba3ec50", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7524.463199999997, "isoelectric_point": 4.504399299621583 }, "id": "GLJLFKCOHC_102", "locus": "GCA013368035_00060" }, { "type": "cds", "sequence": "contig_1", "start": 37783, "stop": 38322, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAACCAGAAGTTCAAAAGTTATTAAAAAATGAAAATTTAAAACAATATTTAACAAAAATAATTAATAATGAGTTAAAAAATGTTCATAATGAAGTTCTAAGTAAAGTCAATACAAATGATAATAATCTTGCCAATTTTATTGTCGATAAATTTGATAAGATTATTGATAATAAATTAATTGACAAAGTTTTCAAATTTATTGAAAATTTAATTGAAAACGGCAGTGAATATGATGCTCAAACATTGCCTGAATTTATCAATAAAATATTGAAAAATCTCGCAGACAGCAAAGGCTTTGATGCGCTAGAACAATTACTAGAAAATGTTTTATCAAATAAAGATATAACCCAAAAATTAGTTGAATTTTTAGTTGCGTTTTTAGAAATTGAAGCAAAAACAAAATTGAATTCTGATGAAATTAACCAATTAACTAATTATTTCAACAAGATTTTACCTACTTCATTGAAATCTAATTTATATAAAAACTTAAAAATTAAATTAACAAATGAATTGAAGTCAATAAATAAATATTCTTGA", "aa": "MKPEVQKLLKNENLKQYLTKIINNELKNVHNEVLSKVNTNDNNLANFIVDKFDKIIDNKLIDKVFKFIENLIENGSEYDAQTLPEFINKILKNLADSKGFDALEQLLENVLSNKDITQKLVEFLVAFLEIEAKTKLNSDEINQLTNYFNKILPTSLKSNLYKNLKIKLTNELKSINKYS", "aa_hexdigest": "095fb76e54a99f4a65d43ae7b27fac11", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20764.685700000027, "isoelectric_point": 7.755191612243652 }, "id": "GLJLFKCOHC_103", "locus": "GCA013368035_00061" }, { "type": "cds", "sequence": "contig_1", "start": 38362, "stop": 38556, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGATACATTAAATGAAGAAAATGTAAAAACTCTATTCTTGATATTAATCAAGAATTTAAATGAATTTGCAAATGTTGTCTTAGCAAACAACGACACAACAACACAAAAACAAAATGAAGGTTCAAAACCAATTAAATTAGACAATGATTTAAATTTTGAGCAAATCTTCTTACTAATTAAAAAATTATGA", "aa": "MIDTLNEENVKTLFLILIKNLNEFANVVLANNDTTTQKQNEGSKPIKLDNDLNFEQIFLLIKKL", "aa_hexdigest": "d583c3f50b1ec02d8d956186b7985824", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7376.437099999998, "isoelectric_point": 4.853277015686035 }, "id": "GLJLFKCOHC_104", "locus": "GCA013368035_00062" }, { "type": "cds", "sequence": "contig_1", "start": 39007, "stop": 39330, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAACTATTTAAAATCAATATTTAGTGACTACTTCAACATTGAAAAAAATAAGGATTTAATTAATAATTTTGTTGATTTACTACTCGAAAAAGATAATAAATCAGAAGGAATAAAATTTTCTGAATTAATAAAAACACTTTCTGTTCTTGCTTCTAAATCTGAACTTGTTAACTACGTCTTTAAAAAATTTGACATTAAATCTATTATATTAAATTACTTAGATTCATTTAAAGACAACAAGCTATCTAACGATGCTGAATTAAATTCACTATTACAAACCTTAATCACCAAAACTAGAAATTACATAGATAGTCAATGA", "aa": "MKNYLKSIFSDYFNIEKNKDLINNFVDLLLEKDNKSEGIKFSELIKTLSVLASKSELVNYVFKKFDIKSIILNYLDSFKDNKLSNDAELNSLLQTLITKTRNYIDSQ", "aa_hexdigest": "23d470e218e4f3c36cab92fdf188faeb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12497.19619999998, "isoelectric_point": 7.827445030212404 }, "id": "GLJLFKCOHC_105", "locus": "GCA013368035_00063" }, { "type": "cds", "sequence": "contig_1", "start": 39742, "stop": 41883, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATAATCTATTTGGCGATTTTTCTAATAAAAAATTACAAGAGCTAATTAAAAATATAAAACAAGCAAACCTGATTACAATTTCTTTGGGATTCAACGACCTTTTTGAACAATCATCATTTTTTAAAGAAGCGTTTGATCTTTTAAGAGAAAACAAAAATAAAGAAGAAATTTTGAAATATTTTTCAAAAATTCTTCTTGAATTTAAAAGAAATTGTAAAAATTTTGAAGATTCATATTCAAAATTAATTTCAATCATTAAACAATATAATCCTAAGGCAAACATTAATCTTGTGGGTTATATATCGCCTTATTTGAAATTAACAAAATTAATTGACAAACAATACAATATTTCATTAACTAATGATTGCATAGAAGAATTAAACAAATCATTAGCAAACATTGCCAAAGCAAACAAGCTAAACTATTTAAACATTAATAATGACATTAAAAATCTCAAAGATAATGCCGATAAACTAAACAACAATTTCTTTGACTGCTTCCCATCTAATAATTTATACAAAAAATTAGCGCAAGATATTTTTATGAAAATGTCATTGCCTAATAATAAATATAAAGAATTAGTTGATAATAATTCTAATCTAAATGAATTTAGCCAATCACTTGAATTTAGCAAAACTCCATCTGAAATTTTTTCAATGATTTTAGGAATAAATGGCAACAATGTAGATTCTTTTGAAAAAGAATATACATTTGAAGAAAATGCAGATATCAAAAATATTAATTCTTCATACAATTCAAACCATATAATCCTTCAATTAAAATCAATGTTAAATTCAAACAAAAACATTGAAGATAAAGAAATTTTGAATACATTTTCAATACTATTCGGATTAATAGGCTTAGACCCTAAAGATTTAAACAGTTTTAATAAGTGTTTATTAGAATCTTTAAAGAATAAAGAAAATAAAAAATCTATCATTAATTTTTTGAATCTTTTTATTGATTCAAAAAATTTAAGCAAATCGTTTTTAGATATTAATAACGCATTAAATAATGCTATTAATAAAAAATCATATGCAGGTTTAAACTTTGAAGAAATTAAAAAAATTGTTTATTCAACAACTACTTCTAAACAAAATATTTATGAAATTTTAAAAGAAATTTTCTATAACAAATCTTTAAGTCAAGATCTTAAAAGAAGTGAATATGCAAAATTATTTAAGGCTATATTTTCAGATGTATTCATCAAAAAAATATTAAAGAGGTTATTGCCTCAAACTACTGTTGATTTATTCAAAAATCTTTTCAATAACAAAGATATTGAAAAAGGTTTAGACAACATTTTTCAAAAAGTGTTGAATTTGTTAATTAATAATTCAGATAAATATTTTAAATTTGAAAAAATTGATGACTTTATTACTACATTGGTTCATGATTCAAGCAAAGATGTAAATGATTTAGTTAGCCTAATAATTAAAGAAATTTCAAAAGATAGCAAACTTGCAAACTATTTGACCGGAAAAATTGTTGATTCTTTATACAAATCATATGATTTAGATTTACTTGAAAAACCAAATATTTCTTTGTTTGTTTCAACATTTTTTGACAACATTTCAAACTTCGATGGTTTATCTCAAATATCAAACATTATTATTCAAATATTAATGAATTCTAAACTAGATGATGACCACCAAAATAGGGATTTAATTTCAGAAATTCTAAATGGAATTCTTTTTGATAAATACGACCTAAATAGGGATAACTCGCTTTTATTTAAAATCCTATCGTTTGAACCAAAAATACAAAATATTAACGACTACAAACAAGGATTAAAACATTTAGCCCTAAAATATGCAATTAAAAATAATGCATTAATGCCAAAAAACATAGGCAAATTAATTGATAAAGATTCAAGAGAAAACATATTAAGATTTTATAAAAATATAGCATTAAATGCAAAGAAGAATTTAAACGAATTTGGTAGCGAAATATTTAAAGATATTATCTCGCTTCTAGTAAATGAATTATTTAAGAATAATAGTTTGTTCAGTCAATTAATTGATCAAATTGGAAATTATGTTGTTATTCAACCTATCGTTGCTTTCATTAAATCAATTAATTATAAAAAAATATTCAACAATGAAGAAGAAATTCAAAAATTTGTTGAAAATGTTTGA", "aa": "MNNLFGDFSNKKLQELIKNIKQANLITISLGFNDLFEQSSFFKEAFDLLRENKNKEEILKYFSKILLEFKRNCKNFEDSYSKLISIIKQYNPKANINLVGYISPYLKLTKLIDKQYNISLTNDCIEELNKSLANIAKANKLNYLNINNDIKNLKDNADKLNNNFFDCFPSNNLYKKLAQDIFMKMSLPNNKYKELVDNNSNLNEFSQSLEFSKTPSEIFSMILGINGNNVDSFEKEYTFEENADIKNINSSYNSNHIILQLKSMLNSNKNIEDKEILNTFSILFGLIGLDPKDLNSFNKCLLESLKNKENKKSIINFLNLFIDSKNLSKSFLDINNALNNAINKKSYAGLNFEEIKKIVYSTTTSKQNIYEILKEIFYNKSLSQDLKRSEYAKLFKAIFSDVFIKKILKRLLPQTTVDLFKNLFNNKDIEKGLDNIFQKVLNLLINNSDKYFKFEKIDDFITTLVHDSSKDVNDLVSLIIKEISKDSKLANYLTGKIVDSLYKSYDLDLLEKPNISLFVSTFFDNISNFDGLSQISNIIIQILMNSKLDDDHQNRDLISEILNGILFDKYDLNRDNSLLFKILSFEPKIQNINDYKQGLKHLALKYAIKNNALMPKNIGKLIDKDSRENILRFYKNIALNAKKNLNEFGSEIFKDIISLLVNELFKNNSLFSQLIDQIGNYVVIQPIVAFIKSINYKKIFNNEEEIQKFVENV", "aa_hexdigest": "ebb3e733d9ee91a4ce1c77599de43ca0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 82743.03840000043, "isoelectric_point": 8.714143180847167 }, "id": "GLJLFKCOHC_106", "locus": "GCA013368035_00064" }, { "type": "cds", "sequence": "contig_1", "start": 41983, "stop": 42351, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGTACAAAAATCCTCAAGGCGATTGTTGCTTTAGGAATTACATCTTCAATTGCTTCTAGTTCATTAGTTGCTATATCATGCAAAGACAAAACTAAGAAGCCGGATACAAAAATAACTCAAAGCGAAAATAAAGTTTCTAAAAAAATTAATTATGTTGCATTAGGCGATGATTTTGCTGCAGGTTATAATAGCAGCTCAAATTACTATTTCAACAATGAATATGATAGCAAAAACGATTTAGTTTTAGGAACTTCGTATCCTTCATACATTGCTAATTTTATAAAACTATTATCTGATAAGCAAACAACATTATCAACTTATTATAATTACGGTTTGCCACTTTCAAATGTTAATGATTGA", "aa": "MKKSTKILKAIVALGITSSIASSSLVAISCKDKTKKPDTKITQSENKVSKKINYVALGDDFAAGYNSSSNYYFNNEYDSKNDLVLGTSYPSYIANFIKLLSDKQTTLSTYYNYGLPLSNVND", "aa_hexdigest": "6022262dd9a09719deabcd105a78d6f9", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13418.996099999986, "isoelectric_point": 9.196302223205567 }, "id": "GLJLFKCOHC_107", "locus": "GCA013368035_00065" }, { "type": "cds", "sequence": "contig_1", "start": 42361, "stop": 42915, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAACAAGATCAGCAGGTTTTTCAACTGTAAATTATTATAATTTTACTCAACCTACAATTATGGTTCACGGTATCCTAATGTTTATTGGATTTTCTCCTGTTTCAACTGCTGGAGGAATTCGTAATATTACAGTGGCTATAATTTTTATGAGTATAATTACTTTAATTAAAAATCGCAAAAACATAAATGCCTTTCATAGACAAATAGGAAAAGAATCTTTAATTAAGGCAGTTAACATTTTTGCAATTGCAATTTTTATAACTTTAATAATGACAATAGTAATATATCATACACTTCCAAGCGATTTAAAAAATGTTTATAATGAGTCATATCCAATGGTCTATGTAATGTTTGAAGTTTTCTCAGCTTTTGGAAACTCAGGACTTAATATTGGATTAACACCAAATTTGGGCATTACTGGAAAAATACTTTTAATTATAACAATGATAGTAGGACAATTAGGAATATCACAATTTCTAAAAATTTTTACTTTTTCAAAATCAAAACCACAAAGATATCAATATATTTATGAAGATATTTCTATTGGTTAG", "aa": "MSTRSAGFSTVNYYNFTQPTIMVHGILMFIGFSPVSTAGGIRNITVAIIFMSIITLIKNRKNINAFHRQIGKESLIKAVNIFAIAIFITLIMTIVIYHTLPSDLKNVYNESYPMVYVMFEVFSAFGNSGLNIGLTPNLGITGKILLIITMIVGQLGISQFLKIFTFSKSKPQRYQYIYEDISIG", "aa_hexdigest": "f37022e26a30740f58d734d53459e704", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20568.131699999998, "isoelectric_point": 9.800693321228025 }, "id": "GLJLFKCOHC_108", "locus": "GCA013368035_00066" }, { "type": "cds", "sequence": "contig_1", "start": 43126, "stop": 43299, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCACTAAGATTTGGAATATTTCATACACTAAGTGCAATAAATAATGCCGGATTAGATATAATTGGCACAAAAAGTTTGCAACCTTATTATCATAATTATTACTTTTTATTTTTAACATCAATTATATTTCTAATTGGTGGAATGGGATTCCCTGTATTCTATGATGTTTGA", "aa": "MSLRFGIFHTLSAINNAGLDIIGTKSLQPYYHNYYFLFLTSIIFLIGGMGFPVFYDV", "aa_hexdigest": "aa88d27af245d632b2199d2d319e902d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6498.521599999998, "isoelectric_point": 6.68991527557373 }, "id": "GLJLFKCOHC_109", "locus": "GCA013368035_00067" }, { "type": "cds", "sequence": "contig_1", "start": 43351, "stop": 43734, "strand": "-", "frame": 2, "gene": "trkG", "product": "potassium transporter TrkG", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9", "UniRef:UniRef90_UPI001F33CDF9" ], "nt": "TTGCTATTAGCAGCCTCATCATTTACAACAACCGGTTTAAGTGGAGTTGCCGTTAGTGAAGTATTTAACATGTTTGGACAGATATTAATAGCTGTCTGTTTTATTCTTGGCGGAATTGGAATTTTTACATTTAAACTTTATATTTTTCAATCATTACTTGGATTAAGAACGAATTTATTTAGTGGCCAAATTCACCAAACTGAAAGAGGCGGTTCTAATATTTATGAAACAAAAAAAATGATAAAAGTTTCAATAAGTTTTTTAATTATTATTTCTATATTATTTTCTTTAATATTTTCTTTAATATTTTATTATGATAATACAGCACTTTTTAGTGATGAAAAAATGTCTCAATTAAATGCATTAAGTAAAAATAATCCATGA", "aa": "MLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNP", "aa_hexdigest": "df4b2a49f6c7991871a4e907aea3c07b", "start_type": "TTG", "rbs_motif": null, "genes": [ "trkG" ], "truncated": "both", "pseudogene": { "start": 43050, "stop": 43791, "observations": { "insertion": [ 43122, 43147, 43193, 43227 ], "mutation": [ 43128, 43353, 43782 ], "stop": [ 43128, 43353, 43782 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F33CDF9", "query_cov": 1.0, "subject_cov": 0.5059760956175299, "identity": 0.992, "score": 215.0, "evalue": 1.26e-68, "gene_start": 1, "gene_end": 127, "reference_start": 20, "reference_end": 146, "reference_sequence": "MLYWEVSHNPSKHISYLDALLLAASSFTTTGLSGVAVSEVFNMFGQILIAVCFILGGIGIFTFKLYIFQSLLGLRTNLFSGQIHQTERGGSNIYETKKMIKVSISFLIIISILFSLIFSLIFYYDNTALFSDEKMSQLNALSKNNPWTFNPYEESIRNPYHNFGMSLRFGIFHTLSAINNAGLDIIGTKVCNLIIIIITFIFNINYISNWWNGIPVFYDVWQKLKASRKTILVIALHYLQNLQLLHTFLHH" }, "paralog": false, "identity": 0.9877551020408163, "score": 430.0, "evalue": 1.19e-155, "description": "Internal stop codon at 43128, 43353, 43782. Frameshift due to insertion around 43122, 43147, 43193, 43227. Nonsense mutation around 43128, 43353, 43782.", "psc": { "uniref90_id": "UniRef90_UPI001F33CDF9", "product": "potassium transporter TrkG", "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F33CDF9", "UniRef:UniRef50_UPI001F33CDF9" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F33CDF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F33CDF9" ], "product": "potassium transporter TrkG" } }, "id": "GLJLFKCOHC_110", "locus": "GCA013368035_00068" }, { "type": "cds", "sequence": "contig_1", "start": 44061, "stop": 44753, "strand": "+", "frame": 3, "gene": "trkA", "product": "Potassium uptake protein KtrA", "db_xrefs": [ "COG:COG0569", "COG:PT", "RefSeq:WP_012855311.1", "SO:0001217", "UniParc:UPI0001BC203B", "UniRef:UniRef100_D1J7H4", "UniRef:UniRef50_D1J7H4", "UniRef:UniRef90_D1J7H4" ], "nt": "ATGAGTTTATTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGTCGGTAGCAAGAACATTATTGGCCGATAGAAATAATAATATAAGGATAGTTTTAGTTGATGAAGATGAAAAACATTTGTTACCTTTTAAAGATGAAGTAGAAATGATCTATGTTGCAGACTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCCGTTATTGTTGGCGCTTCAAATAATATTGAAATAGTTGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGAGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGCCCCGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGTGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGCCAAATTGAAGATATTATTCAAGTTATTGAAATTTGTGCAATGAATGAAAAATAA", "aa": "MSLFKKIREICIIGIGRYGSSVARTLLADRNNNIRIVLVDEDEKHLLPFKDEVEMIYVADCAEQKTLESLNIKDFDAVIVGASNNIEIVAALTECGVKTIIARASTKRHAQVLKQIGVSSIVCPEEEAGKKTAMLVANPALALYSENMVELQDGFVSASIYVKNPAVANKKISELNFRNKYNVLVTMIKRDKHSYIPEGDFEIKLDDLITLIGQIEDIIQVIEICAMNEK", "aa_hexdigest": "18d450a60381e5c4899d963182ccef7d", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC203B", "ncbi_nrp_id": "WP_012855311.1", "uniref100_id": "UniRef100_D1J7H4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC203B", "RefSeq:WP_012855311.1", "UniRef:UniRef100_D1J7H4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7H4", "uniref90_id": "UniRef90_D1J7H4" }, "psc": { "uniref90_id": "UniRef90_D1J7H4", "gene": "TrkA", "product": "Potassium uptake protein KtrA", "uniref50_id": "UniRef50_D1J7H4", "cog_id": "COG0569", "cog_category": "PT" }, "pscc": { "uniref50_id": "UniRef50_D1J7H4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H4" ], "product": "Potassium uptake protein KtrA" }, "genes": [ "ktrA", "trkA" ], "id": "GLJLFKCOHC_111", "locus": "GCA013368035_00069" }, { "type": "cds", "sequence": "contig_1", "start": 44864, "stop": 45073, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAGGCTTCATCAACTAATAATCAAGTTGTAAGAAAACACGATTTTCAATTTTCTGTTGAAGTAAAACCTGGTTCAATAACTAACCAAAAACATTCAGGTAGATGTTGA", "aa": "MAILDNELLKKLEAKYESNLNNKIIENAIFKNGIKASSTNNQVVRKHDFQFSVEVKPGSITNQKHSGRC", "aa_hexdigest": "3609b43cf55c2d29729f13c1fda61b8a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7802.815200000001, "isoelectric_point": 9.463910484313967 }, "id": "GLJLFKCOHC_112", "locus": "GCA013368035_00070" }, { "type": "cds", "sequence": "contig_1", "start": 45110, "stop": 45202, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTAGAAAAAGCAAATGCATATCTAACATGA", "aa": "MKDLNIEDLEFSQNFIHFYDKLEKANAYLT", "aa_hexdigest": "189e783e2c094f0c78409ad88256c469", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3651.0591000000004, "isoelectric_point": 4.581245613098145 }, "id": "GLJLFKCOHC_113", "locus": "GCA013368035_00071" }, { "type": "cds", "sequence": "contig_1", "start": 45218, "stop": 45397, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAACCCTATTTCGGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAAACGATATGGTAGAACAAATTAATTGA", "aa": "MELDNEDRLFRRYNENPISDGGYFEFFLNIASKYGIVPKSAMPESFHSEATNDMVEQIN", "aa_hexdigest": "42c67e7c28df1faf8ac2490492c4e0f8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6878.514100000002, "isoelectric_point": 4.405101585388183 }, "id": "GLJLFKCOHC_114", "locus": "GCA013368035_00072" }, { "type": "cds", "sequence": "contig_1", "start": 45422, "stop": 45811, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTAGAAGTAATTCAACAACATGCTTTGGAAGATGTTTACAACATATTGGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGATAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTACCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCAATTAAACAATTGCAATCTGGTCAATCAATTTGA", "aa": "MREAYKKNHNMSEVEVIQQHALEDVYNILVKSLGMPPKTFIFEYYDKDKKFVRLPEMTPIKFFETYIKQFVENKVDVISDPREIYPYYRQLVAPSVFNSVGGIEYKMLNIPMNELKAAAIKQLQSGQSI", "aa_hexdigest": "855ef915efb1ccd0f0b77dd51921d635", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15145.423999999985, "isoelectric_point": 7.850975990295411 }, "id": "GLJLFKCOHC_115", "locus": "GCA013368035_00073" }, { "type": "cds", "sequence": "contig_1", "start": 46094, "stop": 46195, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGTGAAGAATAA", "aa": "MLIVDKEFLSKEAIEGLSKEAIEISPYDPLCEE", "aa_hexdigest": "eb71dcd39b353ef260716d4fc5eb2555", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3740.2521000000006, "isoelectric_point": 4.216737174987793 }, "id": "GLJLFKCOHC_116", "locus": "GCA013368035_00074" }, { "type": "cds", "sequence": "contig_1", "start": 46308, "stop": 46451, "strand": "+", "frame": 3, "gene": "acpS", "product": "Holo-[acyl-carrier-protein] synthase", "db_xrefs": [ "COG:COG0736", "COG:I", "SO:0001217", "UniRef:UniRef50_D1J7H8", "UniRef:UniRef90_D1J7H8" ], "nt": "ATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTCTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACTAGATGA", "aa": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATR", "aa_hexdigest": "c6a974de7e7b90d325ed84c9ef20712f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "acpS" ], "truncated": "3-prime", "pseudogene": { "start": 46308, "stop": 46635, "observations": { "insertion": [ 46599 ], "mutation": [ 46449 ], "stop": [ 46449 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7H8", "query_cov": 1.0, "subject_cov": 0.42727272727272725, "identity": 1.0, "score": 94.7, "evalue": 3.38e-24, "gene_start": 1, "gene_end": 47, "reference_start": 1, "reference_end": 47, "reference_sequence": "MLGVDLTDIRRFKEYSETLLIKFLHPIEIEQFKKVSITKRADFLATRWAIKEAIFKADNQYYSFNQINITKDEVGRYVFKDFEISTSNEGIYIIAVVLKDRTNSRSYLWI" }, "paralog": false, "identity": 0.9907407407407407, "score": 203.0, "evalue": 1.7e-69, "description": "Internal stop codon at 46449. Frameshift due to insertion around 46599. Nonsense mutation around 46449.", "psc": { "uniref90_id": "UniRef90_D1J7H8", "gene": "AcpS", "product": "Holo-[acyl-carrier-protein] synthase", "uniref50_id": "UniRef50_D1J7H8", "cog_id": "COG0736", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7H8", "UniRef:UniRef50_D1J7H8", "COG:COG0736", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7H8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7H8" ], "product": "Holo-[acyl-carrier-protein] synthase" } }, "id": "GLJLFKCOHC_117", "locus": "GCA013368035_00075" }, { "type": "cds", "sequence": "contig_1", "start": 46632, "stop": 46895, "strand": "+", "frame": 3, "gene": null, "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F", "UniRef:UniRef90_UPI0023AB411F" ], "nt": "ATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGTGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTTAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTCCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGA", "aa": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIM", "aa_hexdigest": "e4346338aaa50bbc249342aa214d6162", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 46632, "stop": 47142, "observations": { "insertion": [ 47085 ], "mutation": [ 46893, 46905 ], "stop": [ 46893, 46905 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AB411F", "query_cov": 1.0, "subject_cov": 0.514792899408284, "identity": 1.0, "score": 182.0, "evalue": 2.14e-57, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MNLRTRIFFRFFGLLHCISFLCAKSRRDFKMPDYYKVVEKNDFLQQKSNAILNHFNISVNVEGFENVPNGPCLIIPNHSTYLDPLIMWSALWNHRDGSKQSKLVNFVARKEITKKKMVKRIADLGQTYYIDTQNPREALATLKDFGQFIKKINHAALFLLKEQEQKMEN" }, "paralog": false, "identity": 0.9763313609467456, "score": 328.0, "evalue": 2.11e-117, "description": "Internal stop codon at 46893, 46905. Frameshift due to insertion around 47085. Nonsense mutation around 46893, 46905.", "psc": { "uniref90_id": "UniRef90_UPI0023AB411F", "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase", "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AB411F", "UniRef:UniRef50_UPI0023AB411F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AB411F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AB411F" ], "product": "1-acyl-sn-glycerol-3-phosphate acyltransferase" } }, "id": "GLJLFKCOHC_118", "locus": "GCA013368035_00076" }, { "type": "cds", "sequence": "contig_1", "start": 46980, "stop": 47237, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAAGAATAGCAGATTTAGGTCAAACTTATTATATTGATACTCAAAATCCTAGAGAAGCATTGGCTACCTTGAAAGATTTCGGCCAATTTATTAAAAGGAATAAATCATGTGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGCGAATTTCATAGTGGCGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAATGCATTGGATTGA", "aa": "MVKRIADLGQTYYIDTQNPREALATLKDFGQFIKRNKSCGVIFAEGTRTKDGKLGEFHSGAFKLAQSSYLTIVPATINNAANALD", "aa_hexdigest": "c448ccf56ce16cd3480acbc017c0ec74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9311.48659999999, "isoelectric_point": 9.205908012390136 }, "id": "GLJLFKCOHC_119", "locus": "GCA013368035_00077" }, { "type": "cds", "sequence": "contig_1", "start": 47283, "stop": 47441, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C", "UniRef:UniRef90_UPI001F16AA7C" ], "nt": "ATGAAGCCAATTCAATTTCAATCACTTGATAGCCGTGTATTAGCAGGACAAGTTAAATCAATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAATGTAAAAGAAGTTAAGAGATAA", "aa": "MKPIQFQSLDSRVLAGQVKSIVASDYIDQTITSNETIKNKYSKRDNVKEVKR", "aa_hexdigest": "4f75dd6599443a7398abc493b3c09032", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 47229, "stop": 47441, "observations": { "mutation": [ 47235 ], "stop": [ 47235 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16AA7C", "query_cov": 1.0, "subject_cov": 0.7428571428571429, "identity": 0.9620000000000001, "score": 92.4, "evalue": 1.14e-23, "gene_start": 1, "gene_end": 52, "reference_start": 19, "reference_end": 70, "reference_sequence": "MDWNRKEKLEVKVTFHKPMKPIQFQSLDSRVLAEQVKSIVASDYIDQTITSNETIKNKYSKRDKVKEVKR" }, "paralog": false, "identity": 0.9428571428571428, "score": 129.0, "evalue": 6.46e-41, "description": "Internal stop codon at 47235. Nonsense mutation around 47235.", "psc": { "uniref90_id": "UniRef90_UPI001F16AA7C", "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16AA7C", "UniRef:UniRef50_UPI001F16AA7C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16AA7C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16AA7C" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5971.7529, "isoelectric_point": 9.69619007110596 }, "id": "GLJLFKCOHC_120", "locus": "GCA013368035_00078" }, { "type": "cds", "sequence": "contig_1", "start": 47445, "stop": 48269, "strand": "+", "frame": 3, "gene": "scpA", "product": "segregation/condensation protein A", "db_xrefs": [ "COG:COG1354", "COG:L", "KEGG:K05896", "RefSeq:WP_175949779.1", "SO:0001217", "UniParc:UPI00158DACE2", "UniRef:UniRef100_UPI00158DACE2", "UniRef:UniRef50_N9U9Z0", "UniRef:UniRef90_D1J7I0" ], "nt": "ATGCCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACTAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCGCTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCACATTTATTACAATTGAAGGCTAGAATGGTATTGCAAGATCCAGAAGTTGAAGAAGAAATTAGACAAGAAAAAAAGAGATTGCTCGAACAGATTGCTGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCAAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCAATTCTTGATGGACATTCCAATAGTTCAAAATTAGTTGTAACTTTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCTGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTGACCTTTAAAGATGTTTTCTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCGGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGCGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAA", "aa": "MPDLEKPIIREISIKEEPNKDQNNQTNSSIFKEKHVFKLKNFDGPLDLLVSLIKEKNISIFDVDLSELANQYLEIIHSLQSYEVDIASEYLVMAAHLLQLKARMVLQDPEVEEEIRQEKKRLLEQIAEYEKFKEISLLLREQEEKRSRLFSKEPEDTQDFVRDIDGSILDGHSNSSKLVVTLRKMFERTYAELIRNITISTIAVSPEEQKERIIKLFKTKLQVTFKDVFSVPSLGHFVITLLAALDLVRQQIIEIYQEENEGEIHFKKGALYEE", "aa_hexdigest": "ab956e8e92eb4b248948f12a52d83209", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158DACE2", "ncbi_nrp_id": "WP_175949779.1", "uniref100_id": "UniRef100_UPI00158DACE2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158DACE2", "RefSeq:WP_175949779.1", "UniRef:UniRef100_UPI00158DACE2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158DACE2", "uniref90_id": "UniRef90_D1J7I0" }, "psc": { "uniref90_id": "UniRef90_D1J7I0", "gene": "ScpA", "product": "segregation/condensation protein A", "uniref50_id": "UniRef50_N9U9Z0", "cog_id": "COG1354", "cog_category": "L", "kegg_orthology_id": "K05896" }, "pscc": { "uniref50_id": "UniRef50_N9U9Z0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9Z0" ], "product": "Segregation and condensation protein A" }, "genes": [ "scpA" ], "id": "GLJLFKCOHC_121", "locus": "GCA013368035_00079" }, { "type": "cds", "sequence": "contig_1", "start": 48259, "stop": 48891, "strand": "+", "frame": 1, "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "db_xrefs": [ "COG:COG1386", "COG:K", "GO:0005515", "GO:0007059", "KEGG:K06024", "RefSeq:WP_104940351.1", "SO:0001217", "UniParc:UPI000CD69D76", "UniRef:UniRef100_UPI000CD69D76", "UniRef:UniRef50_Q6KHG8", "UniRef:UniRef90_D1J7I1" ], "nt": "ATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTAAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTCAAAACATACTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGCGTCAAAGATTATATTAGTGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCCGCAATTGAAGTTGCTGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGTGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAGGTTGGAGTTGCTCAAACTCCAGGCAACCCTATTTTATATGGAATTACTAATAAATTCTATGATTATTTCAAAATAAAGACCTTAGGAGAATTACCTCCTTTTCCTGAATTTAATAAATATAGTAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACAAGAAACAGACAATGATGGAGTTTTAATTTCTAATGAAGGGGCAGAATATGATAAATTTTAA", "aa": "MKNKIIEALLFIQGSEGLSSEQAQQVFKLNTIQEARRLLRDFKTYFNNLERGIKVLEFNDVFKFLTIESVKDYISDLVTITRKQRLSNAAIEVAGIVAYKQPITRGMISNIRGVVSDGIVSSLLEKGIIEEVGVAQTPGNPILYGITNKFYDYFKIKTLGELPPFPEFNKYSSEEEEEIDKDFNLFDSQRQETDNDGVLISNEGAEYDKF", "aa_hexdigest": "e08438d076668dc77a88bafba75a0699", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000CD69D76", "ncbi_nrp_id": "WP_104940351.1", "uniref100_id": "UniRef100_UPI000CD69D76", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CD69D76", "RefSeq:WP_104940351.1", "UniRef:UniRef100_UPI000CD69D76" ] }, "ips": { "uniref100_id": "UniRef100_UPI000CD69D76", "uniref90_id": "UniRef90_D1J7I1" }, "psc": { "uniref90_id": "UniRef90_D1J7I1", "gene": "scpB", "product": "SMC-Scp complex subunit ScpB", "uniref50_id": "UniRef50_Q6KHG8", "cog_id": "COG1386", "cog_category": "K", "kegg_orthology_id": "K06024", "go_ids": [ "GO:0005515", "GO:0007059" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHG8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHG8" ], "product": "Segregation and condensation protein B" }, "genes": [ "scpB" ], "id": "GLJLFKCOHC_122", "locus": "GCA013368035_00080" }, { "type": "cds", "sequence": "contig_1", "start": 48863, "stop": 49711, "strand": "+", "frame": 2, "gene": "rluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "db_xrefs": [ "COG:COG0564", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXK9", "UniRef:UniRef90_D1J7I2" ], "nt": "ATGAAGGGGCAGAATATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATATTTGGTAAAGGTTTGCGACAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATATACGTGTAAATGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACCTCCAAACATATTGACAATAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATTGACAAGGCACCAAATATTGCAATGCATTCTGAAGAAAATTGTCTAGATGATCAAGTTTTAAAATATCTAAATTTTAAAAAAGTTTCTTCGTTTAGACCTGCTTCGATTGGTAGGATAGATAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAATAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATATTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGCGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGCAAAAAACATCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTGCATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGA", "aa": "MKGQNMINFKAKKDDEGRILFKYLVKVCDNVPISRIERLFREKDIKVNNIRVNDKQYKVKEGDLISIYGLIETSKHIDNKANTNINFKVIYEDKNLLIIDKAPNIAMHSEENCLDDQVLKYLNFKKVSSFRPASIGRIDKKTSGLVVYAKNYKTLVEFENKQNKFDKIYQFVSDFDEDILDITVRLKKDIKNEKMIVDNNFGVKAQTIFYKEGNRKFAKIITGKKHQIRATLAYLKTPILGDIKYGGKFAKRMYLHSYSIKFHDLSDDFKEYNEQVFICYPK", "aa_hexdigest": "52dbd08ba06bda3bc47490d78fd34155", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J7I2", "query_cov": 0.9822695035460993, "subject_cov": 0.9964028776978417, "identity": 0.996, "score": 545.0, "evalue": 5.58e-196, "valid": true, "gene": "RluA", "product": "Ribosomal large subunit pseudouridine synthaseC", "uniref50_id": "UniRef50_A0A448ZXK9", "cog_id": "COG0564", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXK9" ], "product": "Ribosomal large subunit pseudouridine synthase C" }, "genes": [ "rluA" ], "id": "GLJLFKCOHC_123", "locus": "GCA013368035_00081" }, { "type": "cds", "sequence": "contig_1", "start": 49734, "stop": 50036, "strand": "+", "frame": 3, "gene": "gatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "db_xrefs": [ "COG:COG0721", "COG:J", "RefSeq:WP_012855320.1", "SO:0001217", "UniParc:UPI0001BC2044", "UniRef:UniRef100_D1J7I3", "UniRef:UniRef50_D1J7I3", "UniRef:UniRef90_D1J7I3" ], "nt": "ATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTGCTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCACATCAATGAATCACCCATTTGTTTCGAACAACTAAGAAGTGATGAACCGGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTACGTAATTATGAAGAAGGTAATAAATGATAAATAA", "aa": "MEKEEIKKLANDLLFEPSEEVIKLTNNLLNSIEKGLKDLEEFDLQDIKPMSHINESPICFEQLRSDEPDKSFYLNKQDLLNNASEHDENYVIMKKVINDK", "aa_hexdigest": "e5f45cfef76f2cb5e32c174fb203ef47", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2044", "ncbi_nrp_id": "WP_012855320.1", "uniref100_id": "UniRef100_D1J7I3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2044", "RefSeq:WP_012855320.1", "UniRef:UniRef100_D1J7I3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I3", "uniref90_id": "UniRef90_D1J7I3" }, "psc": { "uniref90_id": "UniRef90_D1J7I3", "gene": "GatC", "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C", "uniref50_id": "UniRef50_D1J7I3", "cog_id": "COG0721", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7I3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I3" ], "product": "Glutamyl-tRNA(Gln)amidotransferase subunit C" }, "genes": [ "gatC" ], "id": "GLJLFKCOHC_124", "locus": "GCA013368035_00082" }, { "type": "cds", "sequence": "contig_1", "start": 50026, "stop": 50928, "strand": "+", "frame": 1, "gene": "gatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "db_xrefs": [ "COG:COG0154", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A449AI30", "UniRef:UniRef90_A0A6A8PZD5" ], "nt": "ATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCATTTGTTTTTAAAGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAAGATAATTATGCGGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTTGCAAGAACAAATTTGGACGAATTTGGTATGGGCGGTAGTGGAGAATATTCATGCAATGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGGGGTTCTTCATCGGGTGCCGCTGCGACTTTTTCTAAAAATATTTCGTTTGCAATTGGATCGGACACAGGCGATTCAGTTAGAAAACCTGCATCAAATATTGGCGAGGTTGGATTTAAACCAAGTTATGGTGCTATAAGCCGTTATGGATTGTCCGCTTTTGCTTCGTCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAACTCTCTCATCAGTATTGTTTAAGCAAGATTTAAAACATGATTTGACATCAATTGATTTGTCGTTTAATGCAGATAGTGTTATTGAAACAAAACCACATAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTTGTAAAAATTTCAATAAATGAAAAGTTACTGCAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCATCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACTGGCAATTCTTGA", "aa": "MINKGNLKEAILQLNADKNNAVSFVFKDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGEVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSIDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNS", "aa_hexdigest": "a4ac03b2b303c49a614bc1c158d52eb9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "gatA" ], "truncated": "3-prime", "pseudogene": { "start": 50026, "stop": 51231, "observations": { "mutation": [ 50926 ], "stop": [ 50926 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PZD5", "query_cov": 1.0, "subject_cov": 0.6787330316742082, "identity": 0.993, "score": 568.0, "evalue": 4.69e-202, "gene_start": 1, "gene_end": 300, "reference_start": 1, "reference_end": 300, "reference_sequence": "MINKGNLKEAILQLNADKNNAVSFVFKDAQLKEQGPLANCVFTIKDNYADLNVIESASSRFLSNFRPSYQSTILKLLNEAGATCVARTNLDEFGMGGSGEYSCNGLIKNPLNSNYLVGGSSSGAAATFSKNISFAIGSDTGDSVRKPASNIGQVGFKPSYGAISRYGLSAFASSLDTVAYFTHNVQDAITLSSVLFKQDLKHDLTSVDLSFNADSVIETKPHKIAYLDCFDKLDISVADAYKKLLNKLEKMNIELVKISINEKLLQAIDTVYKVIAFSESSSNLANLVGVEFGNRITGNSWEETFNLSRTEGIGKMVQSRMILGSYFLEDNNQIKYFVRAKKMRRYLSNYFTNVHKSADIFIYPASNMSAPLIGKLYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE" }, "paralog": false, "identity": 0.9900249376558603, "score": 778.0, "evalue": 3.79e-287, "description": "Internal stop codon at 50926. Nonsense mutation around 50926.", "psc": { "uniref90_id": "UniRef90_A0A6A8PZD5", "gene": "GatA", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA", "uniref50_id": "UniRef50_A0A449AI30", "cog_id": "COG0154", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PZD5", "UniRef:UniRef50_A0A449AI30", "COG:COG0154", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AI30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AI30" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit A" } }, "id": "GLJLFKCOHC_125", "locus": "GCA013368035_00083" }, { "type": "cds", "sequence": "contig_1", "start": 50971, "stop": 51354, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01425.27" ], "nt": "ATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTGAGCAATTATTTCACTAATGTTCATAAAAGTGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATTATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACAGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTGGCAATTGACGGAGAAATATATAAAGATTGCAAGATGCTAAGTCACGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAA", "aa": "MVQSRMILGSYFLEDNNQIKYFVKAKKMRRYLSNYFTNVHKSADIFIYPASNMSAPLIGKLYEPSYMDYILTNSNLTGNPSLTLKLGIDKNNNLPFNLAIDGEIYKDCKMLSHALFIERVLEGEKNE", "aa_hexdigest": "ccf6ff9afc8feeef637dd2333e80cd70", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01425.27", "name": "Amidase", "length": 119, "aa_cov": 0.8661417322834646, "hmm_cov": 0.9915966386554622, "evalue": 1.835527876681944e-10, "score": 35.77909851074219, "start": 2, "stop": 111 } ], "seq_stats": { "molecular_weight": 14657.770899999983, "isoelectric_point": 8.590106010437015 }, "id": "GLJLFKCOHC_126", "locus": "GCA013368035_00084" }, { "type": "cds", "sequence": "contig_1", "start": 51413, "stop": 51691, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02934.20" ], "nt": "ATGTTTTCTCCAGTTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCCGGAAGTTTGCCTCTTTTAAATTCTAGTGCCATAATTAAGGCAATTAAATTAGCCAAGGCGCTAGAAATGGAAATAGACCCATTAGTTAGATTTGATAGGAAAAATTATTTTTATCCCGATTTAACAAAGGGATATCAAATTACTCAACAATTTCATCCAATTGGTAAAAATGGTTTGATAAGGGCAAAGGTTCAAAATTCATGA", "aa": "MFSPVQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNS", "aa_hexdigest": "6ba59d61015d10e89bd2db2e241135a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02934.20", "name": "GatB_N", "length": 90, "aa_cov": 0.9782608695652174, "hmm_cov": 1.0, "evalue": 6.084353361022387e-33, "score": 109.54290008544922, "start": 1, "stop": 90 } ], "seq_stats": { "molecular_weight": 10344.70249999999, "isoelectric_point": 7.964891624450684 }, "id": "GLJLFKCOHC_127", "locus": "GCA013368035_00085" }, { "type": "cds", "sequence": "contig_1", "start": 51719, "stop": 52768, "strand": "+", "frame": 2, "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "db_xrefs": [ "COG:COG0064", "COG:J", "EC:6.3.5.6", "EC:6.3.5.7", "GO:0016884", "KEGG:K02434", "SO:0001217", "UniRef:UniRef50_A0A449AA70", "UniRef:UniRef90_D1J7F4" ], "nt": "ATGGAAGAAGATACTGCTAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTTCCTTTAATTGAAATAGTTTCTAATCCTGTAATGCACAGTGCAGAGGATGCAATGGCTTATGTTGAAGCCATAAGACAAACAGCATTAGTTTTAGACATTAGTGATGCAAAATTAAATGAAGGAAGCTTAAGAACTGACGTTAATTTATCAGTAAGAAGAAGAGGAGAAAATATTCTTAATACTCGCACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCAAAAGGTGATGCAATTAGTTATAAATATTTTCCAGATCCAAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATAAAAAAGAATTAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTGTTGAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCAAGAAGGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAA", "aa": "MEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRRGENILNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYKKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLSFKADDARRVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE", "aa_hexdigest": "75c95883c64d7985dc43cea1bb6f001c", "start_type": "ATG", "rbs_motif": null, "genes": [ "gatB" ], "truncated": "5-prime", "pseudogene": { "start": 51419, "stop": 52768, "observations": { "mutation": [ 51689 ], "stop": [ 51689 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7F4", "query_cov": 1.0, "subject_cov": 0.7378435517970402, "identity": 0.986, "score": 657.0, "evalue": 7.13e-236, "gene_start": 1, "gene_end": 349, "reference_start": 125, "reference_end": 473, "reference_sequence": "MNNDWEIVIGIEIHIELNTKTKMFSPIQNEYGDEANTNVSNIDLAYPGSLPLLNSSAIIKAIKLAKALEMEIDPLVRFDRKNYFYPDLTKGYQITQQFHPIGKNGLIRAKVQNSWKTFSIERIHMEEDTAKSIHENGLTYLNYNRCGVPLIEIVSNPVMHSAEDAMAYVEAIRQTALVLDISDAKLNEGSLRTDVNLSVRRKGENVLNTRTEIKNLNSLSNIKKAIEYESNFQIQMYENNLTFNQETKRFDEKMQKTVSMRSKGDAISYKYFPDPNLPYIQLQQKFIDDIKIEEIPYQKELRYLKNGLNQVQISQLLNNISYAKYIDSINSKDFKKATNVFFSDIVTYLNSNNKNIENLLFKADDARKVVEYLIDNRINKNNIAEIIKIKDQNPNLNIESIIKDNGFLIEIKEIDLNELVQEILNENPNLETEFKNNYNRAQKFMQGQIMKKTMGKANLKNLDEIIKNRFNNE" }, "paralog": false, "identity": 0.9844097995545658, "score": 874.0, "evalue": 0.0, "description": "Internal stop codon at 51689. Nonsense mutation around 51689.", "psc": { "uniref90_id": "UniRef90_D1J7F4", "gene": "gatB", "product": "Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatB", "ec_ids": [ "6.3.5.6", "6.3.5.7" ], "uniref50_id": "UniRef50_A0A449AA70", "cog_id": "COG0064", "cog_category": "J", "kegg_orthology_id": "K02434", "go_ids": [ "GO:0016884" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7F4", "EC:6.3.5.6", "EC:6.3.5.7", "UniRef:UniRef50_A0A449AA70", "COG:COG0064", "COG:J", "KEGG:K02434", "GO:0016884" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449AA70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AA70" ], "product": "Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B" } }, "id": "GLJLFKCOHC_128", "locus": "GCA013368035_00086" }, { "type": "cds", "sequence": "contig_1", "start": 53043, "stop": 53198, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGCTCGGTTGATGAAAAGTATGAAGTGGTGCTAAAGAACGTAATTTTGGGTCAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACATTATGA", "aa": "MYLFLSFIPISILMLGIIGSVDEKYEVVLKNVILGQIIPGISEVIPSFSTL", "aa_hexdigest": "69d05ef375dc0ac833d6cb7a1bce4907", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5611.733599999998, "isoelectric_point": 4.406579399108887 }, "id": "GLJLFKCOHC_129", "locus": "GCA013368035_00087" }, { "type": "cds", "sequence": "contig_1", "start": 53147, "stop": 53353, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATCCTTTAATTCTCGATTTTCACGCTCTGAATGGAGATTTGTGTTCATATAAAGCATCTATTGAAAAAATAAATTTTGAATATCCCTTCGATGCTAAAATTAGTACTGATAATGCAAATAAGATTAAAGCAATTGCATTGCCTGTCGAACCTCATAATGTACTAAATGATGGAATTACTTCTGAAATTCCGGGAATAATTTGA", "aa": "MNPLILDFHALNGDLCSYKASIEKINFEYPFDAKISTDNANKIKAIALPVEPHNVLNDGITSEIPGII", "aa_hexdigest": "071e74e9bbe3cabfe0c888d365e43dc1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7468.471200000001, "isoelectric_point": 4.7775674819946286 }, "id": "GLJLFKCOHC_130", "locus": "GCA013368035_00088" }, { "type": "cds", "sequence": "contig_1", "start": 53373, "stop": 53519, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTGTCTTTAATCTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGTCTTGGAAAATTTGATGTCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGA", "aa": "MILSLILLGLTAFMVFLIDKVGLGKFDVDIKKMKLADFNLTTEFYLIY", "aa_hexdigest": "2f9291aa44645f72288f27463f5e7d79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5516.7020999999995, "isoelectric_point": 5.955214500427247 }, "id": "GLJLFKCOHC_131", "locus": "GCA013368035_00089" }, { "type": "cds", "sequence": "contig_1", "start": 53532, "stop": 53843, "strand": "+", "frame": 3, "gene": null, "product": "YhjD/YihY/BrkB family envelope integrity protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95", "UniRef:UniRef90_UPI002FE35A95" ], "nt": "TTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCTGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCATTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTACATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGA", "aa": "MFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSF", "aa_hexdigest": "f4d5323e4ec5f05e3006e13736be685c", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 53234, "stop": 53923, "observations": { "insertion": [ 53282, 53838, 53851 ], "stop": [ 53328, 53517 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE35A95", "query_cov": 1.0, "subject_cov": 0.42738589211618255, "identity": 0.99, "score": 194.0, "evalue": 7.69e-61, "gene_start": 1, "gene_end": 103, "reference_start": 113, "reference_end": 215, "reference_sequence": "MRFYRQCNCFNLICAIGINFSIEGIFKIYFSIDALYEHKSPFRAWKSRIKGFIVSVFVCLILSLILLGLTAFMVFLIDKVGLGKFDADIKKMKLADFNLTTEFYLIYWFTTILFLPVFTYLGFLFYFVYAPNFKLKMSQAHPGALIAAIPTAIFILIFGSLTSLIPYKKFGIVASFMYIILLLSFTSYFIYMGIIVNSSFYKTFINLPTVEKHSFWKNKNFNLKSFDFFANFSLFIVINDI" }, "paralog": false, "identity": 0.9649122807017544, "score": 412.0, "evalue": 2.33e-149, "description": "Internal stop codon at 53328, 53517. Frameshift due to insertion around 53282, 53838, 53851.", "psc": { "uniref90_id": "UniRef90_UPI002FE35A95", "product": "YhjD/YihY/BrkB family envelope integrity protein", "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE35A95", "UniRef:UniRef50_UPI002FE35A95" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE35A95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE35A95" ], "product": "YhjD/YihY/BrkB family envelope integrity protein" } }, "id": "GLJLFKCOHC_132", "locus": "GCA013368035_00090" }, { "type": "cds", "sequence": "contig_1", "start": 54063, "stop": 54812, "strand": "+", "frame": 3, "gene": null, "product": "ECF transporter S component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4", "UniRef:UniRef90_D1J7I4" ], "nt": "GTGGGTGTTTTGATTGCTATCTCAGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCTATAAGTGCCTTACCAAGTATAAAATTTAGCTTCATTGGTTTGCCAATAAAAATAACTGGTTTTATTTTTGGACCAATTGTTGGTATGATAACTGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGGTTTGTTCCCGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGCAACTCCGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAGCCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTAGGTATTCAAGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTCAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTCTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATCTTATTGGCGTGA", "aa": "MGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLA", "aa_hexdigest": "9db49f7cfba2e9009cbeac8f6e97cb26", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 53964, "stop": 54968, "observations": { "mutation": [ 54003, 54039, 54810 ], "stop": [ 54003, 54039, 54810 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I4", "query_cov": 0.9959839357429718, "subject_cov": 0.7425149700598802, "identity": 0.992, "score": 430.0, "evalue": 3.97e-150, "gene_start": 2, "gene_end": 249, "reference_start": 35, "reference_end": 282, "reference_sequence": "MNKLPGGLRHYIYWARSQTNISYRKWNSKRIAFVGVLIAISVVFFLISVRIMPISALPSIKFSFIGLPIKITGFIFGPIVGMITGILADIISFALVPTYYNVLYTIAVAVSGFVPGFAAYYFFNINELFFSRTYRIFKYKETIDYFKEQYDIALTIGNSEDMQYFSEKIAFYEVKIIILECKKKPIAMINFSFISTLIILGIQVLIILAIFSNLDPSIFEKNRFVKNKKFYMFLTSIGFIGMMIFVSLYRIFLKKKFETFLEIMAIISFSAVLEIVNVILLAWADTSTLKTDFWVNFTAQSLFSPVKIFFNLAIILATYKVVAPLIKSKEGGHL" }, "paralog": false, "identity": 0.9910179640718563, "score": 624.0, "evalue": 3.64e-229, "description": "Internal stop codon at 54003, 54039, 54810. Nonsense mutation around 54003, 54039, 54810.", "psc": { "uniref90_id": "UniRef90_D1J7I4", "product": "ECF transporter S component", "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I4", "UniRef:UniRef50_D1J7I4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I4" ], "product": "ECF transporter S component" } }, "id": "GLJLFKCOHC_133", "locus": "GCA013368035_00091" }, { "type": "cds", "sequence": "contig_1", "start": 54968, "stop": 55378, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACTAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACGCTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAATGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGTTACATTGAATTTCAAACAAACTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGCTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACGGAAGAATTTGTTTCTCAGTGA", "aa": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDNETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQ", "aa_hexdigest": "2e06ececaf3fec5fb826703db58a6b0e", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15535.109999999982, "isoelectric_point": 6.56259593963623 }, "id": "GLJLFKCOHC_134", "locus": "GCA013368035_00092" }, { "type": "cds", "sequence": "contig_1", "start": 55406, "stop": 56323, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311", "UniRef:UniRef90_UPI001E424311" ], "nt": "TTGCTTCAAAGCGAATTTGCAAAGTTACAATATGGTGTTGAAATTGAACTCGGAAGCAAATTAGATGACTTATTCAGTGAATTAAGTCAATTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTCTTTTTAAGAAATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCGACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAAATTTATAAACCTTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAATTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATTTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCGTTTATTCACTTTTATAAAAATCTATATTTTAATTGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCTTTTGAATTATTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCTAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATATTACAAGAGAAATTCAAACGTCTAATAACTATGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACGGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGA", "aa": "MLQSEFAKLQYGVEIELGSKLDDLFSELSQFDLRNIDAKNLKFDTLFKKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKFLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNCLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDITREIQTSNNYDLNIGEFGLANKAFEKLKTVESKYKDAKNEYF", "aa_hexdigest": "eb7e47aebf7a92ddf5a6ac384d58b952", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 55106, "stop": 56368, "observations": { "mutation": [ 55376, 56321 ], "stop": [ 55376, 56321 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E424311", "query_cov": 1.0, "subject_cov": 0.6475583864118896, "identity": 0.987, "score": 570.0, "evalue": 2.8e-202, "gene_start": 1, "gene_end": 305, "reference_start": 147, "reference_end": 451, "reference_sequence": "MKKIAISSLKVTNLKSINVVNEQFKNLINNLSFTLNANNILGFVGGDSETNNLIFNYLTNNTINPKRYQGYIEFQTNLGEYSSIYKNSNYQNNIAFGFYNDILNNDLNEDNSYRNIKKYINHGAVIGALTEEFVSQWKPVFQDYKLLLQSEFAKLQYGVEIELGSKLDDLFSELSQFDLRNIDAKNLKFDTLFKKFKDIEILYRTHEMKLFAFVNATIAKYNKGLSFLKRKEYLDSKKEYEEVIKKIYKPFSKPNRFLNRVRFFSKFLETKKSSKSSIKKFVNKLRRDLNIENYYYKYELKKTIKQSSFIHFYKNLYFNGLLLKFINKNLKAILGLKEAKSFELLYEIFDLKNNITNEVNVISNIDNQKELKNEIKAVIKNNFLNDVMPYIERIKASSYEHTQEVINDVTREIQTSNNYDLNIGEFGLANKAFEKLKIVESKYKDAKNEYFWNYKTEGNAILKETKSFKKF" }, "paralog": false, "identity": 0.9857142857142858, "score": 821.0, "evalue": 8.51e-304, "description": "Internal stop codon at 55376, 56321. Nonsense mutation around 55376, 56321.", "psc": { "uniref90_id": "UniRef90_UPI001E424311", "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E424311", "UniRef:UniRef50_UPI001E424311" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E424311", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E424311" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 36437.675800000055, "isoelectric_point": 9.44798679351807 }, "id": "GLJLFKCOHC_135", "locus": "GCA013368035_00093" }, { "type": "cds", "sequence": "contig_1", "start": 56417, "stop": 57103, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAAAACTAAAAATTTTTAATTTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTGCAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGTGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCAAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATAAATAATCCTTCACTAATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGCGAAACATTGAATAAATTGAACAAATTCGTTTTAGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGGAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTACAAAGACGAGAAGACTCAAGAGGATTTAAGCTTTTATATGTCTCAAGAAGACAATATGGAAAATATATACAAATATGAAATAGAGCCATACCACTTTGTTTGA", "aa": "MKLKLKIFNLINLIKIKEFNNSVLNEQVKKIKENLQFLKILRNEFDSYKYVVSDFKTASKKQIKLFVSKINIYKIIAKANIDVNFLVEKLGSLSDDEKIRLECEKILINNPSLIIIGPNISKLPYEKQCETLNKLNKFVLDNHKMAIYFLDNINIAQKITTDLYIINSSKIIEQGKTSQLIRNPINPIVKNMLNYKDEKTQEDLSFYMSQEDNMENIYKYEIEPYHFV", "aa_hexdigest": "84e060ec3f72abf4ca9bfe4e7df5e508", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26868.150400000028, "isoelectric_point": 9.107529258728025 }, "id": "GLJLFKCOHC_136", "locus": "GCA013368035_00094" }, { "type": "cds", "sequence": "contig_1", "start": 57164, "stop": 57349, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTATTTGTTCCACAAAATACTTTTCAAAGTAAAGGAAATAAAACAATAGACAATAAGGAACCATTTGAAGAAACAACTCTTATAAATTTAATCGATGACGAAGATAACGAAGTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGATAGATGCAAAAAATGA", "aa": "MSLFVPQNTFQSKGNKTIDNKEPFEETTLINLIDDEDNEVRNLNMEKTFNHKIVEKDRCKK", "aa_hexdigest": "1e25dcf793365e257c491f8402487ca6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7229.058300000001, "isoelectric_point": 5.284571266174315 }, "id": "GLJLFKCOHC_137", "locus": "GCA013368035_00095" }, { "type": "cds", "sequence": "contig_1", "start": 57356, "stop": 57517, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTTACCATTATTTTACCACCCCCAAATGTAACTGGCAATCTTCACATTGGGCACGCCCTAGACAATTATATTTCTGATACAATAATAAGATTTAAAAAGTTACAGGGATATGATGTATTGTGA", "aa": "MKIFQTHDESKKPFTIILPPPNVTGNLHIGHALDNYISDTIIRFKKLQGYDVL", "aa_hexdigest": "a7f9fee12811d4164b5043ad99892ddd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 47, "aa_cov": 0.8867924528301887, "hmm_cov": 1.0, "evalue": 1.3666899092419504e-17, "score": 58.95497131347656, "start": 6, "stop": 52 } ], "seq_stats": { "molecular_weight": 6078.990000000001, "isoelectric_point": 8.123548316955567 }, "id": "GLJLFKCOHC_138", "locus": "GCA013368035_00096" }, { "type": "cds", "sequence": "contig_1", "start": 57878, "stop": 58360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTACTACATAAAATATCCAATTAAGAATTCAGAAAACTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTCTCAGATGTAGCTTTAGCAATAAACCCGCAAGATAGTAGAGCAAAACAATTAATAAAAGAAATAATAATACATCCATTAACAAATAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTTCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAATGATTTAGCAGGTGAATTTAAGGGATTAGATCGTTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGATATAGTGAAAGATCAAAGGCCCCAATTGAAATTTTAGTTCAGCCACAATGA", "aa": "MYYIKYPIKNSENFLEVATTRPETMFSDVALAINPQDSRAKQLIKEIIIHPLTNKEIPIILSNKIDPNFGSGIMKVSAHAVDDIEIIIENKLPINECINKDGKLNDLAGEFKGLDRFEAREKIAHLLSQKGLLIKEEEVVSNIGYSERSKAPIEILVQPQ", "aa_hexdigest": "d85c91520d1b6260c1346dc77dcdec9e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 60, "aa_cov": 0.36875, "hmm_cov": 1.0, "evalue": 2.2790810043422393e-11, "score": 38.9304313659668, "start": 47, "stop": 105 } ], "seq_stats": { "molecular_weight": 18040.648399999987, "isoelectric_point": 5.462306785583496 }, "id": "GLJLFKCOHC_139", "locus": "GCA013368035_00097" }, { "type": "cds", "sequence": "contig_1", "start": 58742, "stop": 58996, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "ATGTATTTCTTTAGCTTGTATTTTATGGATCAAAAACCTTTTGAACATCTCTTATTGCATGGATTAGTTAGAGATTCGCAAGGAAGAAAAATGTCTAAATCGCTTGGCAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGCACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGTGCTAAACTATTTGCTAATAAATTCTGA", "aa": "MYFFSLYFMDQKPFEHLLLHGLVRDSQGRKMSKSLGNGINPIQVIDEYGSDVLRMSLIFNCTPGLDINYSDEKIQGAKLFANKF", "aa_hexdigest": "2c3b7d7103de409c769653338b70449a", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001D01CCB6", "query_cov": 0.9642857142857143, "subject_cov": 0.9310344827586207, "identity": 0.58, "score": 106.0, "evalue": 1.57e-28, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A3B0PEY5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 69, "aa_cov": 0.8214285714285714, "hmm_cov": 0.9855072463768116, "evalue": 2.9348604457957386e-22, "score": 74.33128356933594, "start": 1, "stop": 69 } ], "seq_stats": { "molecular_weight": 9653.03739999999, "isoelectric_point": 6.705830192565918 }, "id": "GLJLFKCOHC_140", "locus": "GCA013368035_00098" }, { "type": "cds", "sequence": "contig_1", "start": 59282, "stop": 59536, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTATATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCTTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATACAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCAGTATAGATTTAAAAATAATTAACAAATTAAGCAATTTTTCTTGA", "aa": "MPFLTDYVFEKLYHEELLCSNLQTFALNKESSNNTPKIISLITALRKYREDKKISKSIELFFYLNNLVFSSIDLKIINKLSNFS", "aa_hexdigest": "375f586a6051fff53124d3f451dfcd1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9885.36759999999, "isoelectric_point": 8.733677101135253 }, "id": "GLJLFKCOHC_141", "locus": "GCA013368035_00099" }, { "type": "cds", "sequence": "contig_1", "start": 59699, "stop": 59815, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAGGCTAATAAAAATACAATCAATGAAAAATTAGCAAATTTAAAATTGCATAAAGAAAAATTAGAGTTTTATAAAAACCAACTTAAAGAAAAACAAGAAAAAATAAATTAA", "aa": "MEKANKNTINEKLANLKLHKEKLEFYKNQLKEKQEKIN", "aa_hexdigest": "8e4876d05b6b34e5405b5ce13d428f5c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4629.3415, "isoelectric_point": 9.574602699279783 }, "id": "GLJLFKCOHC_142", "locus": "GCA013368035_00100" }, { "type": "cds", "sequence": "contig_1", "start": 59895, "stop": 60119, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "GTGACTGAATTCAGTAACTTTGAAGCTGGAACAAAAGGAATTGCAACCGCTATTGCTCAAAATAGAGATTGCTATTCAGTAGTTGGCGGGGGTGACTCTGTTACAGCAATAAACAAATTAGGTTATCAAGATAAATTTAGTTTTATTTCAACTGGCGGTGGAGCATCAATAGAATTTGTCCAAGGAAAGAAATTGCCTGGAATAGAAGCAATTCAAGACAAATAA", "aa": "MTEFSNFEAGTKGIATAIAQNRDCYSVVGGGDSVTAINKLGYQDKFSFISTGGGASIEFVQGKKLPGIEAIQDK", "aa_hexdigest": "3ab3b07e362735e0cc9e197cf2aaa980", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030BBA936", "query_cov": 0.972972972972973, "subject_cov": 0.8470588235294118, "identity": 0.542, "score": 84.3, "evalue": 6.8e-20, "valid": false, "product": "phosphoglycerate kinase", "ec_ids": [ "2.7.2.3" ], "uniref50_id": "UniRef50_A0A166IH01", "kegg_orthology_id": "K00927" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 64, "aa_cov": 0.8513513513513513, "hmm_cov": 1.0, "evalue": 3.922345348453172e-23, "score": 77.40230560302734, "start": 1, "stop": 63 } ], "seq_stats": { "molecular_weight": 7736.569799999998, "isoelectric_point": 5.1351415634155275 }, "id": "GLJLFKCOHC_143", "locus": "GCA013368035_00101" }, { "type": "cds", "sequence": "contig_1", "start": 60135, "stop": 60659, "strand": "-", "frame": 3, "gene": "pgk", "product": "Phosphoglycerate kinase", "db_xrefs": [ "COG:COG0126", "COG:G", "SO:0001217", "UniRef:UniRef50_P62415", "UniRef:UniRef90_D1J7I6" ], "nt": "GTGTATGTTAATGATGCTTTTGGAACTGCACACAGAGCTCATGCATCAACATATGGAATTTCACAATATTCTAAAGAATCAGCATTAGGATTTTTAATGAATAATGAAGTTGAACACCTTCAAAAATTATTAGTAGGATTTAAACGTCCTTTTGTTGCAATAATTGGTGGAGCAAAAGTAAGCGATAAAATCAAAGTTCTAGAAAAGTTGTTTGAACTAGCAGATAAGGTTTTAATTGTTGGTGGTATGGCATATACATTCCAAAAAGCCTTAAATAGAAAAATTGGAACTTCTATTTTTGAAGAAGATAAAGTTGAAATGGCAAAAGAATATTTAAACAAATATAAAGATAAAATTGTTTTGCCAATTGACAATGCTTATGTTGATGAATTCGCTGACAAAACTCCTAAATTTACAAGTCCAGAAAATCCTGATATTCCTGACGGAAATATGGGATTAGATATTGGACCAGAAAGTATTAAATTATTTGCAAGTATTATTAAAAATGCTAAAACTATCTTTTGA", "aa": "MYVNDAFGTAHRAHASTYGISQYSKESALGFLMNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNRKIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKNAKTIF", "aa_hexdigest": "b74ab664ea2915be8d5274519089bf86", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [ "pgk" ], "truncated": "both", "pseudogene": { "start": 59895, "stop": 60959, "observations": { "mutation": [ 60137, 60677 ], "stop": [ 60137, 60677 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I6", "query_cov": 1.0, "subject_cov": 0.43828715365239296, "identity": 0.9940000000000001, "score": 340.0, "evalue": 4.24e-115, "gene_start": 1, "gene_end": 174, "reference_start": 144, "reference_end": 317, "reference_sequence": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEDLAKYWASLGDVYVNDAFGTAHRAHASTYGISQYSKESALGFLMNNEVEHLQKLLVGFKRPFVAIIGGAKVSDKIKVLEKLFELADKVLIVGGMAYTFQKALNRKIGTSIFEEDKVEMAKEYLNKYKDKIVLPIDNAYVDEFADKTPKFTSPENPDIPDGNMGLDIGPESIKLFASIIKNAKTIFWNGPAGVTEFSNFEAGTKGIATAIAQNRDCYSVVGGGDSVTAINKLGYQDKFSFISTGGGASIEFVQGKKLPGIEAIQDK" }, "paralog": false, "identity": 0.9943502824858758, "score": 685.0, "evalue": 2.45e-253, "description": "Internal stop codon at 60137, 60677. Nonsense mutation around 60137, 60677.", "psc": { "uniref90_id": "UniRef90_D1J7I6", "gene": "Pgk", "product": "Phosphoglycerate kinase", "uniref50_id": "UniRef50_P62415", "cog_id": "COG0126", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I6", "UniRef:UniRef50_P62415", "COG:COG0126", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_P62415", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P62415" ], "product": "Phosphoglycerate kinase" } }, "id": "GLJLFKCOHC_144", "locus": "GCA013368035_00102" }, { "type": "cds", "sequence": "contig_1", "start": 60675, "stop": 61088, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00162.24" ], "nt": "ATGAAGAAAACCATTGAAGACCTTCAATTAGAAAATAAAAAGGTTATTTTAAGAGTTGATTTTAACGTTCCTATTTCAAACAATACTATTATGGACACAAAAAGAATTGATGAAGAATTGCCAACAATCAAATACATATTGAGTAAAAATGCTTCTTTAATCATTCTATCTCATTTAGGAAGAGTTAAAACAGAAGAAGACAAGAAAACTAAATCTTTAGAAATTGTAGCTAAAAAATTGGCGCAATTATTAAATAGAGACGTTAAATTTATTAACGAAAATAGAAGTGAAGCAGTTACAAATGCTGCTAAAAACTTAAAACCTGGAGAAATTTTGGTTTTAGAAAATACTCGTTTTCAAGATTTAAATAATAAAGCAGAATCTAATAATGATGAAGATCTTGCAAAATATTGA", "aa": "MKKTIEDLQLENKKVILRVDFNVPISNNTIMDTKRIDEELPTIKYILSKNASLIILSHLGRVKTEEDKKTKSLEIVAKKLAQLLNRDVKFINENRSEAVTNAAKNLKPGEILVLENTRFQDLNNKAESNNDEDLAKY", "aa_hexdigest": "515c239c6121c3015d4e53237a087b3c", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00162.24", "name": "PGK", "length": 133, "aa_cov": 0.9635036496350365, "hmm_cov": 0.9548872180451128, "evalue": 1.9529229592859677e-40, "score": 134.35263061523438, "start": 5, "stop": 136 } ], "seq_stats": { "molecular_weight": 15706.88729999998, "isoelectric_point": 8.729293251037596 }, "id": "GLJLFKCOHC_145", "locus": "GCA013368035_00103" }, { "type": "cds", "sequence": "contig_1", "start": 61200, "stop": 61376, "strand": "+", "frame": 3, "gene": null, "product": "YARHG domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4", "UniRef:UniRef90_D1J7I7" ], "nt": "ATGGCAAAAATTTTAAATGCTCAAAAACAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTTCATTATTTTAAAGAATCTAGATGA", "aa": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESR", "aa_hexdigest": "c5016de7e57661d77b4a08d5e9f9db54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 61200, "stop": 61607, "observations": { "mutation": [ 61374, 61524 ], "stop": [ 61374, 61524 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7I7", "query_cov": 1.0, "subject_cov": 0.4264705882352941, "identity": 1.0, "score": 114.0, "evalue": 1.98e-31, "gene_start": 1, "gene_end": 58, "reference_start": 1, "reference_end": 58, "reference_sequence": "MAKILNAQKQKDTRTLSYDPEKDSLTLLISEFQLYNKNTKFINDYSEMEKFHYFKESRWMKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9851851851851852, "score": 245.0, "evalue": 1.65e-85, "description": "Internal stop codon at 61374, 61524. Nonsense mutation around 61374, 61524.", "psc": { "uniref90_id": "UniRef90_D1J7I7", "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7I7", "UniRef:UniRef50_A0A448ZYF4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYF4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYF4" ], "product": "YARHG domain-containing protein" } }, "id": "GLJLFKCOHC_146", "locus": "GCA013368035_00104" }, { "type": "cds", "sequence": "contig_1", "start": 61377, "stop": 61526, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F36E0B6", "UniRef:UniRef90_UPI001F36E0B6" ], "nt": "ATGAAAACAATGGATGAAAAATTGCAAGATCAAGTGCAAGAATTTTCAAAAAGGCAAAAAAAATTAAATGCCAAATATAAAAGTTACATTGAATATTCTAAATGGTCTGAATCGCCATATGCAACAAAAACTAAACCGCTTACAAAATGA", "aa": "MKTMDEKLQDQVQEFSKRQKKLNAKYKSYIEYSKWSESPYATKTKPLTK", "aa_hexdigest": "78f0925452087919a2505a5dae3b3c70", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 61341, "stop": 61607, "observations": { "mutation": [ 61374, 61524 ], "stop": [ 61374, 61524 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F36E0B6", "query_cov": 1.0, "subject_cov": 0.5568181818181818, "identity": 0.9390000000000001, "score": 85.1, "evalue": 1.29e-20, "gene_start": 1, "gene_end": 49, "reference_start": 13, "reference_end": 60, "reference_sequence": "MEKFHYFKESRWMKTMDEKLQDQVQEFSKGKKLNAKYKSYIEYSKWSESPYAAKTKPLTKWNRIIIISVFIILVIIMLLIVIGLNKLW" }, "paralog": false, "identity": 0.9431818181818182, "score": 142.0, "evalue": 7.55e-46, "description": "Internal stop codon at 61374, 61524. Nonsense mutation around 61374, 61524.", "psc": { "uniref90_id": "UniRef90_UPI001F36E0B6", "uniref50_id": "UniRef50_UPI001F36E0B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F36E0B6", "UniRef:UniRef50_UPI001F36E0B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F36E0B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F36E0B6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5933.764700000001, "isoelectric_point": 9.698575401306151 }, "id": "GLJLFKCOHC_147", "locus": "GCA013368035_00105" }, { "type": "cds", "sequence": "contig_1", "start": 61751, "stop": 62209, "strand": "+", "frame": 2, "gene": null, "product": "DAC domain-containing protein", "db_xrefs": [ "EC:2.7.7.85", "KEGG:K18672", "SO:0001217", "UniParc:UPI0001BC204C", "UniRef:UniRef100_D1J7I8", "UniRef:UniRef50_D1J7I8", "UniRef:UniRef90_D1J7I8" ], "nt": "TTGGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCCCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATCACCATCGAAAATAAAGATAATCTTGATTTGCTAAGAACTGACGGCGTGATATTAAATGCTAACATTTCTGCTTCTCTAATTATTTCTATTTTTAATAAACACGCTCCACTTCACGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGCGCAAGACACCGCGCTGCTATGGGTATTTCAGAAATTTGTGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGTGGAATTACAATAGCTAAAAATGGAACTTTTAGCCCTGTTTTACTAGAAAATCTACAAGAAAGATTAGTCAATATTTTTAAGGATAATAATTAA", "aa": "MGESTKIRIIYQLKEALEFLSKTKTGAIITIENKDNLDLLRTDGVILNANISASLIISIFNKHAPLHDGAIIIRNNKIYYAATYYKITKKSIDNKYGARHRAAMGISEICDATTIVVSEENGGITIAKNGTFSPVLLENLQERLVNIFKDNN", "aa_hexdigest": "0118841dcdf0adc1110f00f51ad17697", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC204C", "uniref100_id": "UniRef100_D1J7I8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC204C", "UniRef:UniRef100_D1J7I8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7I8", "uniref90_id": "UniRef90_D1J7I8" }, "psc": { "uniref90_id": "UniRef90_D1J7I8", "product": "DAC domain-containing protein", "ec_ids": [ "2.7.7.85" ], "uniref50_id": "UniRef50_D1J7I8", "kegg_orthology_id": "K18672" }, "pscc": { "uniref50_id": "UniRef50_D1J7I8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7I8" ], "product": "DAC domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_148", "locus": "GCA013368035_00106" }, { "type": "cds", "sequence": "contig_1", "start": 62783, "stop": 63976, "strand": "+", "frame": 2, "gene": "tuf", "product": "elongation factor Tu", "db_xrefs": [ "COG:COG0050", "COG:J", "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414", "RefSeq:WP_012855329.1", "SO:0001217", "UniParc:UPI0000129DB6", "UniRef:UniRef100_P22679", "UniRef:UniRef50_Q8Y422", "UniRef:UniRef90_P22679" ], "nt": "ATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTACGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCTGACTATGTTAAAAACATGATTACCGGTGCTGCCCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATAGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGATGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGACGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATATGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATTACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTAAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACACACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTAACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGAAGAACTGTTGGTGCCGGATCAGTTACAAAAATTCTTAAATAA", "aa": "MAKLDFDRSKPHVNIGTIGHVDHGKTTLTAAIATVLAKKGLAEARDYASIDNAPEEKARGITINTSHIEYQTEKRHYAHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLARQVGVPKIVVFLNKIDMFKDDEREEMVGLVEMDVRSLLSEYGFDGDNAPIIAGSALKALQGDPEYEKGILELMDAVDTYIEEPKRETDKPFLMAVEDVFTITGRGTVATGRVERGVLQLNEEVEIVGLKPTKKTVVTGIEMFRKNLKEAQAGDNAGLLLRGIDRSEVERGQVLAKPKTIVPHTQFEATVYVLKKEEGGRHTPFFHNYKPQFYFRTTDVTGGIEFKPGREMVVPGDNVELTVTLIAPIAIEEGTKFSIREGGRTVGAGSVTKILK", "aa_hexdigest": "094091eaca3165ab26beac6d706d2680", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0000129DB6", "ncbi_nrp_id": "WP_012855329.1", "uniref100_id": "UniRef100_P22679", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000129DB6", "RefSeq:WP_012855329.1", "UniRef:UniRef100_P22679" ] }, "ips": { "uniref100_id": "UniRef100_P22679", "uniref90_id": "UniRef90_P22679" }, "psc": { "uniref90_id": "UniRef90_P22679", "gene": "tuf", "product": "elongation factor Tu", "uniref50_id": "UniRef50_Q8Y422", "cog_id": "COG0050", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0003924", "GO:0005525", "GO:0005829", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q8Y422", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8Y422" ], "product": "Elongation factor Tu" }, "genes": [ "tuf" ], "id": "GLJLFKCOHC_149", "locus": "GCA013368035_00107" }, { "type": "cds", "sequence": "contig_1", "start": 64252, "stop": 72909, "strand": "+", "frame": 1, "gene": null, "product": "DUF6161 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q1J5", "UniRef:UniRef90_A0A6A8Q1J5" ], "nt": "ATGAATGAAAAAAAGAAAAAAATTGCCATTCCATTGGCAATTTTATGTGGCGGTTTAGCAATTGCTACAACAGCGCTTATTGCAATAAAGGCTCGCCGTCATAAAATTGCTAATCAATTGCAAAAAGAAAATTTATTGCAAAATTTTAAAAAATTACAAAAGCAATTAAATGAATTGCTAGGATATAAAATTGTTAATGAAATAAATGTTTTTCATGAACAAGAAGTTTTGCAAGGATCACTAAAAATTAATAATAAATCTGAAACCAAAGTAATTGAAGAAGAAACTTTAAGGTTAAAAGATGCTATTACTTTATTAATATCAAAAATAAAAAATCAAATAAATCAAAAAGAATTGGAATTTGCTAAATTTAATGAAATAAAAGATAAATTGCAAGAATATATCAAAAACGAGTTATCGAAACAAGAATACGAACATATTAAACAAAATATCGAAAATGAATTAAATAAATATACTCCAATTAGTCTTGAATCAACTTTAATCGAAATACAAAATGCAGCAAATAATCTAATTAAATTGTTAAATGAATCAACCAAAGAAAAAGATAACATTGATAATTTAAATGCCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAGAAATCATTAGATGCTGAAATTAAAAATGCAAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAACAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTTATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTGATGCTAAAAAAGCAAAGGAATTAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCATTGCAAACAGCTCAATCTGCTAAAACTGAAGCTGATGGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTTAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGACATTATTGCTGCAAATCAAGCCTTACAACAAGCATTAAATACTGCAAAGGCTAAAAAATCAAGTATAGATAATGAATTAAAACCTCTAAAGGATGATTTGCAAAGTAAAATTGATGAATTTGGACCTATAAGAAATACCAATTTTTCATGA", "aa": "MNEKKKKIAIPLAILCGGLAIATTALIAIKARRHKIANQLQKENLLQNFKKLQKQLNELLGYKIVNEINVFHEQEVLQGSLKINNKSETKVIEEETLRLKDAITLLISKIKNQINQKELEFAKFNEIKDKLQEYIKNELSKQEYEHIKQNIENELNKYTPISLESTLIEIQNAANNLIKLLNESTKEKDNIDNLNAKEQLKASISQANQLLPQLSDNDSEIAKAKKSLDAEIKNANQAVASNNTASMQSAKTTLDAKIAQVNQQLQQFNKEKENKFNELKQTRSQIEAFINANKTNQNYADLIAKLTDAKKAKELVSESSNKSDIIAANDELKQALQTAQSAKTEADGTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIALNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANDELKQALQTAQSAKTEADRTNGDAKAKLSTSLTTAKELVKKLVDSDGKIQQAKTQLNQEIKKVESAIASNNTAAIQALQNPFDTKISEIQNQLTEFNKDKTNKFNELKQTRSQIEAFINANKTNQNYADLIAKLTNAKKAKESVSESSNKSDIIAANQALQQALNTAKAKKSSIDNELKPLKDDLQSKIDEFGPIRNTNFS", "aa_hexdigest": "dd5e0339ffbcda5417059ec876f724e1", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A6A8Q1J5", "query_cov": 1.0, "subject_cov": 0.8472834067547724, "identity": 1.0, "score": 4884.0, "evalue": 0.0, "valid": true, "product": "Family of unknown function (DUF6161)", "uniref50_id": "UniRef50_A0A6A8Q1J5" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q1J5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q1J5" ] }, "genes": [], "id": "GLJLFKCOHC_150", "locus": "GCA013368035_00108" }, { "type": "cds", "sequence": "contig_1", "start": 73051, "stop": 73179, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGACTATAACCGAAGAATTTGGAAAAGTTGAAACTAAAAATAGTAACATCGGATATAGATTGTTTAAATTGGCACAAGCTGAACAATTTAATAACAGTGATGTTGATAAATTAAAGAACGCTTGA", "aa": "MKTITEEFGKVETKNSNIGYRLFKLAQAEQFNNSDVDKLKNA", "aa_hexdigest": "21c2179ce380e467939d766403930307", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4821.380200000002, "isoelectric_point": 8.064946556091307 }, "id": "GLJLFKCOHC_151", "locus": "GCA013368035_00109" }, { "type": "cds", "sequence": "contig_1", "start": 73264, "stop": 73425, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCACTCAAGAATCTACAATTAAAAAAGTTATAGTAAATATTCAAGCACATATAAGAAATAACCTTAACTCACAATATAGATTAGAAGCTGACAAGCTTATTGCCAATATGAAGAGAGGTTATGGAGATAAAGTTGGTATAGAAAGCTTACAAAAATGA", "aa": "MSTQESTIKKVIVNIQAHIRNNLNSQYRLEADKLIANMKRGYGDKVGIESLQK", "aa_hexdigest": "ee97439d1a30047a63fc4db015103412", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6045.904100000001, "isoelectric_point": 9.77445468902588 }, "id": "GLJLFKCOHC_152", "locus": "GCA013368035_00110" }, { "type": "cds", "sequence": "contig_1", "start": 73435, "stop": 73542, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACGATAGTGTATTGAGTGTAGATGATAGTTTGAAAGATGACTTTAACAAAGCACTTAGAGTATTAGTTGGAGATTACACTAAAAACCCTCCTGTTTTATCATGA", "aa": "MNDSVLSVDDSLKDDFNKALRVLVGDYTKNPPVLS", "aa_hexdigest": "ccf5bb038ae3dcd782907304eb7a8568", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3866.3088000000007, "isoelectric_point": 4.391858100891113 }, "id": "GLJLFKCOHC_153", "locus": "GCA013368035_00111" }, { "type": "cds", "sequence": "contig_1", "start": 74152, "stop": 74385, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594649D", "UniRef:UniRef90_UPI001594649D" ], "nt": "TTGTTAACAATTCCTGAATTTATTAAAGTTGCTAAAACAACAGGAAGCAATAACTTACGATCACTGACAGAACAATTTAAAATTACATTGTTAGACATTGATAAGAATTTAAAAGAAGTAAAGAAAATACTAGATGAAAATAAAACATTAAATAGTGATAACATAGCCAAATTAAACGAAAAAATAAGATTACTTGTAGATAGATCTCGCGAATTAAAAGAATCATTAAGATGA", "aa": "MLTIPEFIKVAKTTGSNNLRSLTEQFKITLLDIDKNLKEVKKILDENKTLNSDNIAKLNEKIRLLVDRSRELKESLR", "aa_hexdigest": "e78f4c72eb57b99dfbe1e35d73095bf1", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 73900, "stop": 74469, "observations": { "mutation": [ 73954, 74041, 74383 ], "stop": [ 73954, 74041, 74383 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001594649D", "query_cov": 1.0, "subject_cov": 0.4074074074074074, "identity": 0.805, "score": 112.0, "evalue": 1.09e-29, "gene_start": 1, "gene_end": 77, "reference_start": 85, "reference_end": 161, "reference_sequence": "MKKNKKELEDIIRSNFYLWEKNEINKYISELTNKQVDLRSNINFERNWESIKDKLDNLNIKENVSLLKEVIINNSNAQYSINKILLTIPEFIKVAKTTRSNNLRLLSEQFKYTLLSIDNSWKEVKKILDENKTLSRENITKLNEKIKLFVDRSRELKESLRWFDNFHENISDQRIKNKLDEVRELLKNV" }, "paralog": false, "identity": 0.873015873015873, "score": 311.0, "evalue": 2.05e-110, "description": "Internal stop codon at 73954, 74041, 74383. Nonsense mutation around 73954, 74041, 74383.", "psc": { "uniref90_id": "UniRef90_UPI001594649D", "uniref50_id": "UniRef50_UPI001594649D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001594649D", "UniRef:UniRef50_UPI001594649D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001594649D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594649D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8939.342299999997, "isoelectric_point": 9.570863533020017 }, "id": "GLJLFKCOHC_154", "locus": "GCA013368035_00112" }, { "type": "cds", "sequence": "contig_1", "start": 74677, "stop": 75852, "strand": "+", "frame": 1, "gene": null, "product": "Lmp protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410EEB1", "UniRef:UniRef90_UPI00093C2025" ], "nt": "TTGACCTTTATTGCCGGGGGAGTTGCCATTACTGCTTTTGTTTTAATAATGTGTAGATTTTGCAAGAAACCTAATAAGCAAATAAAAGATCGAACCTCTTTAAATTTATTTAATAAGGCGCAAAGTGAATTGAAAGAATTAATTAATTCTCCAATAGCCAAACTATGCCAAACCGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGTTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCAAAACAAGAATTAGAAAAATTCAACATGGCTATGTTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTGTAGATACCAAAAATTCAATCAATGCTTTAAATAATGCTAAAAAATTGCCTTTTAATTCTATTGAACAAGTCAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCGGAACCTTCTAAATATGTTGATAGTTCTAGAGAATTGCAAATATTTAATGATTCAAATGTTGAAGCAAAATCAACACTTGATGATATTGATAAAAAGATTGATGCTGCAATAAGAGCAAGCAATGATTTAAATGAAAAAATTAAAAATTACAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAGATAAAATTAATGAAAAGATTAAAGAAGTAAAAGGCGTTGGAAAAAATTCAACAATCAAAGAATTGCAAGTAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAATAAAGTTAGAGAAATTCAAAGATCTAAAATATTGTTGGTTGATGCTGAATATATTGCAAGATTTTTAAGATCAGGAAATGGTGAACCATTTAGACAAAAGAATGAATTTAATGAATATTCCGGAACAGAATTTTTCAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAAGATTTAAATTCTCTTGACAGTGTTGATTATAAAGTAATATGA", "aa": "MTFIAGGVAITAFVLIMCRFCKKPNKQIKDRTSLNLFNKAQSELKELINSPIAKLCQTAIEQKVLNDSNKINEKSTDEEISKNTEYLNSSIALLKNKLDLAKQELEKFNMAMFQLRSLVNNKDSKSVDTKNSINALNNAKKLPFNSIEQVKSESLTLKNNIDELKLKLKEVKKSRLAEAKNKLKAIIDSEPSKYVDSSRELQIFNDSNVEAKSTLDDIDKKIDAAIRASNDLNEKIKNYKSARLSDLESSRNDLSNFIANDLKDPVYKEIADKINEKIKEVKGVGKNSTIKELQVAIKTLADAKEKAIKEKAWIDRVGINKVREIQRSKILLVDAEYIARFLRSGNGEPFRQKNEFNEYSGTEFFKLKNELERVSNLKDLNSLDSVDYKVI", "aa_hexdigest": "a48312735a07a81e4f608b097eba883e", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI00093C2025", "query_cov": 0.9846547314578005, "subject_cov": 0.9344660194174758, "identity": 0.943, "score": 655.0, "evalue": 1.66e-235, "valid": true, "product": "Lmp protein", "uniref50_id": "UniRef50_UPI002410EEB1" }, "pscc": { "uniref50_id": "UniRef50_UPI002410EEB1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410EEB1" ], "product": "Lmp protein" }, "genes": [], "id": "GLJLFKCOHC_155", "locus": "GCA013368035_00113" }, { "type": "cds", "sequence": "contig_1", "start": 76117, "stop": 76380, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAAGCTTCTCACTGTTGACATTCGCGATAAATTTAGAGAAGTTTATATAACTGAAGAAGCAAAGAAAAATAATCTTGCATATAAAGTTGTTGATTTATTAAATAAATTAGAATCATTTGATTTGAACAATAGTAAATTAGATAAACAAGAAATTAAAGATTTTAAAGAAAAATTAAGTGCAAAAAATCTAGAAATTGAAAAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGCGCATATAACCCTGATTTATGA", "aa": "MLKLLTVDIRDKFREVYITEEAKKNNLAYKVVDLLNKLESFDLNNSKLDKQEIKDFKEKLSAKNLEIEKYLSSEETFKDYRAYNPDL", "aa_hexdigest": "2b086619a0888ffef37405172c17f4b0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10395.756599999992, "isoelectric_point": 5.530570411682128 }, "id": "GLJLFKCOHC_156", "locus": "GCA013368035_00114" }, { "type": "cds", "sequence": "contig_1", "start": 76381, "stop": 76539, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D", "UniRef:UniRef90_UPI001F1864BA" ], "nt": "ATGAATAATTTAAGTTATGATATAAAAGAACAAGAAAAAATTTTGAATGATATAAATTCTAAGATTTCTTTAATTGAAGAAAACTCATTTACAAACCAAGAATTAAAGAATACTTTTGCTGAATTAAAACAAAAATTATCAATATTGAATAAAAACTAA", "aa": "MNNLSYDIKEQEKILNDINSKISLIEENSFTNQELKNTFAELKQKLSILNKN", "aa_hexdigest": "a32f5185b3e791b81d8473f199567427", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 76243, "stop": 76539, "observations": { "mutation": [ 76378 ], "stop": [ 76378 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F1864BA", "query_cov": 1.0, "subject_cov": 0.5306122448979592, "identity": 1.0, "score": 95.9, "evalue": 1.05e-24, "gene_start": 1, "gene_end": 52, "reference_start": 47, "reference_end": 98, "reference_sequence": "MNNSKLDKQEIKDFKEKLSAKNLEIEKYLSSEETFKDYRAYNPDLWMNNLSYDIKEQEKILNDINSKISLIEENSFTNQELKNTFAELKQKLSILNKN" }, "paralog": false, "identity": 0.9795918367346939, "score": 189.0, "evalue": 5.66e-64, "description": "Internal stop codon at 76378. Nonsense mutation around 76378.", "psc": { "uniref90_id": "UniRef90_UPI001F1864BA", "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F1864BA", "UniRef:UniRef50_UPI002735871D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002735871D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002735871D" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6099.829, "isoelectric_point": 5.221025276184081 }, "id": "GLJLFKCOHC_157", "locus": "GCA013368035_00115" }, { "type": "cds", "sequence": "contig_1", "start": 76763, "stop": 77236, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAGAATTTTAGAACATTTTTATTTTAAAAGTCCTGAAACAAATAAAGATTATTTATTTTTTGAATATAATGACGAAACTATTTCAAAAGAATTTAAAGATATCGAAACTGAATTATTATCATATCAAAAGCAAAAACAAGATAAATTAGATAAATATTTATATAGTTTTACAATAAATCATGACACCGAAAATATAGATGAAGAAAAATTAATAAAATCTAATGACTATTATTTCAACCCTGAAGAAGACATGATGTATCTTCATTATCATTTTGGACAATTACTAGATCAAAAAATTAATTCAGTTTTTGAATATTACAATAAAAAAGCAAAAGCCTTATTACAAAAAATTCAAAATAACGCTAATTATTCAAAACAAAAAGAAAAATTGCAAAAATTGCTTTCAAAAAATATTTCTTATAGACTTATACTTTCGGGTGAAACAAATCGAGAAAATATCTACTATAATTGA", "aa": "MRILEHFYFKSPETNKDYLFFEYNDETISKEFKDIETELLSYQKQKQDKLDKYLYSFTINHDTENIDEEKLIKSNDYYFNPEEDMMYLHYHFGQLLDQKINSVFEYYNKKAKALLQKIQNNANYSKQKEKLQKLLSKNISYRLILSGETNRENIYYN", "aa_hexdigest": "9d15667ca85747996b9651e2bbf716c7", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19275.40570000001, "isoelectric_point": 5.850517082214354 }, "id": "GLJLFKCOHC_158", "locus": "GCA013368035_00116" }, { "type": "cds", "sequence": "contig_1", "start": 77255, "stop": 77380, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAAGAAAAATTTATTAATTTAAAACCCGCACAAATAAAGCAATTCTTTAAAGAATATGAAGAAAGAAAAGAAGCATTTTATAGAATTAATGAAATGGCGTTAGTTTTAGAAAGAACATGA", "aa": "MPKEKFINLKPAQIKQFFKEYEERKEAFYRINEMALVLERT", "aa_hexdigest": "a9f85296b5ae8063f8a66f9de5ce5be6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5107.946, "isoelectric_point": 9.156267356872558 }, "id": "GLJLFKCOHC_159", "locus": "GCA013368035_00117" }, { "type": "cds", "sequence": "contig_1", "start": 77405, "stop": 78088, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTCATTCTACAAAATAAATAGTATGAATTCAAAACTTAATAATGACATTTATATAAAAAATATTGAATTTTATGAATATGCAGAAAAAATCTGTGCTTCAGTCAAGCAACTAAAATCCTTGCATTCTAAATTAATTAATATAAAAGCAAATAACAATATAGAGCAAGATCTAGATTCATTAATATCAAAATGTGAAATCAGTATAAACAAAAACAAAAATATTGATAATAGCCTTAATTTAAATGACTTGAAAGAGAGATATTCTGAAATATCAGCAGCTATAAAAAACGTTTCAAATTCCTTAGGCCAATATGAAAATACAATATCAAAAAAGATTACTGAATATAAAGAAAATATTTCAAAAATAGAAAACATTAAAAATAGTTATTTTTCACATATTGATAATGGGGAAGAATATTACAAATTGTTTAAAGATGAAATTGAATCATTTAAAAAGGATTTTAAAAAGATTCAATCTATAAAAAGTTATGATGAATATTCAAAAGAAACAGATGCTAAAATTAATTATTTAAAACAAGAAATTAAAGAACACGCTAGATTAAGCAAAGAAATTAAGGAAAATGGTGAAAACAAAGTAAATAAAGATTCTGGCGAAGACACATTTTTGGAACTTAGAAACTCAAAATTCAAAGAACCAAAATCATTTTTATTACTTTGA", "aa": "MDSFYKINSMNSKLNNDIYIKNIEFYEYAEKICASVKQLKSLHSKLINIKANNNIEQDLDSLISKCEISINKNKNIDNSLNLNDLKERYSEISAAIKNVSNSLGQYENTISKKITEYKENISKIENIKNSYFSHIDNGEEYYKLFKDEIESFKKDFKKIQSIKSYDEYSKETDAKINYLKQEIKEHARLSKEIKENGENKVNKDSGEDTFLELRNSKFKEPKSFLLL", "aa_hexdigest": "da31e63838322de30fbb6adc56102663", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26639.707600000034, "isoelectric_point": 7.617982292175294 }, "id": "GLJLFKCOHC_160", "locus": "GCA013368035_00118" }, { "type": "cds", "sequence": "contig_1", "start": 78420, "stop": 79007, "strand": "-", "frame": 3, "gene": "tdk", "product": "thymidine kinase", "db_xrefs": [ "COG:COG1435", "COG:F", "EC:2.7.1.21", "GO:0004797", "GO:0005524", "RefSeq:WP_012855332.1", "SO:0001217", "UniParc:UPI0001BC2078", "UniRef:UniRef100_D1J7J2", "UniRef:UniRef50_Q5ZZT0", "UniRef:UniRef90_D1J7J2" ], "nt": "ATGTATAAAACATTTAATGATGGAATGATAGAAGTTATAACTGGACCAATGTTTAGTGGAAAAAGTGAAGAATTATTAAGGAGAGTTAGAACCTTAGAATATGCTAAGATGCTACCGCTTTTAATAAAGCCCGAATATGATAATCGTTTTTCTGATGATGAAATAGTAAGCCGTTGTGGTGTGAGAAGAAAAACACATTCATTAAAAAACATTAACGAAGTTTATGAATTACTTAAACAAGATAAATATAAGGCAATAATAATTGACGAAGCCCATTTTTTTGGAAATTCATTAATTGAAGTCGCTGATGACTTAGCAAATAAGGGATATTTAGTAATTGTAGCTGGCCTAGACCAAGATTATCTAAGAAAACCTTTTGGACCAATTCCCGCCTTGATGGCCATGGCTGAACGAGTAACTAAATTACAAGCAATTTGCGTAGTATGCCAACATGCTGCTTCAACAAGTTTTAGAAAAGATTCTTCAAATGAACAAAACTTAATTGGCGACTATAATGAATATGAAGCAAGATGCAGAAAGTGCCACATTATGGGTCAAAAACAAAAGGAATTACTAAAAAATAAGTAA", "aa": "MYKTFNDGMIEVITGPMFSGKSEELLRRVRTLEYAKMLPLLIKPEYDNRFSDDEIVSRCGVRRKTHSLKNINEVYELLKQDKYKAIIIDEAHFFGNSLIEVADDLANKGYLVIVAGLDQDYLRKPFGPIPALMAMAERVTKLQAICVVCQHAASTSFRKDSSNEQNLIGDYNEYEARCRKCHIMGQKQKELLKNK", "aa_hexdigest": "3f302cbda5058def0254b6f728fb0f1e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC2078", "ncbi_nrp_id": "WP_012855332.1", "uniref100_id": "UniRef100_D1J7J2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2078", "RefSeq:WP_012855332.1", "UniRef:UniRef100_D1J7J2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7J2", "uniref90_id": "UniRef90_D1J7J2" }, "psc": { "uniref90_id": "UniRef90_D1J7J2", "gene": "Tdk", "product": "thymidine kinase", "ec_ids": [ "2.7.1.21" ], "uniref50_id": "UniRef50_Q5ZZT0", "cog_id": "COG1435", "cog_category": "F", "go_ids": [ "GO:0004797", "GO:0005524" ] }, "pscc": { "uniref50_id": "UniRef50_Q5ZZT0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q5ZZT0" ], "product": "Thymidine kinase" }, "genes": [ "tdk" ], "id": "GLJLFKCOHC_161", "locus": "GCA013368035_00119" }, { "type": "cds", "sequence": "contig_1", "start": 79082, "stop": 79972, "strand": "+", "frame": 2, "gene": "ychF", "product": "redox-regulated ATPase YchF", "db_xrefs": [ "COG:COG0012", "COG:J", "GO:0005524", "GO:0005525", "GO:0016887", "SO:0001217", "UniRef:UniRef50_P47270", "UniRef:UniRef90_D1J7J3" ], "nt": "ATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACATTGTTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAACCTAACATTGCAATAGTTAATTTAGAAGATCCAAGGCTTTATGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCTGGATTAGTTGCAGGAGCTTCAAAAGGAGAAGGCTTAGGCAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAATAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATCAATAGAATTGGTAAAAAAGCTCAAAATTCAGGTGATAAAGAATTAAAATTTGAATTTGAAGTTGCCTTAAAAATACGTAATGCGTTAGAAAATGAAATTATGGTTAAAAACATAGATTTGAGCCCAGAAGAACAAGCCGTAATCAAATCATATCAATTACTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGCGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTGTGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCGGGCAAAATCGAAGCAAGGGCATGA", "aa": "MSLKAGIVGLPNVGKSTLFSALTLNEAEMANYAFTTIEPNIAIVNLEDPRLYELAKIVQTEKIVPATFQFVDIAGLVAGASKGEGLGNKFLANIREVDAIVHVIRCFENADIMHVNNKIDPISDLKTINIELILADLQTIENVINRIGKKAQNSGDKELKFEFEVALKIRNALENEIMVKNIDLSPEEQAVIKSYQLLTAKPTIYVANLDSDSFKNLESAKYYQELNKYLQDKKEILIPICIKLEYELCKFNDEEKEMFLNEYNIECSGLNLIIKKSFYLLNQATYFTAGKIEARA", "aa_hexdigest": "06c5a034fdb6d708412edd0a017277dd", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J7J3", "query_cov": 1.0, "subject_cov": 0.8065395095367848, "identity": 0.99, "score": 565.0, "evalue": 4.38e-202, "valid": true, "gene": "ychF", "product": "redox-regulated ATPase YchF", "uniref50_id": "UniRef50_P47270", "cog_id": "COG0012", "cog_category": "J", "go_ids": [ "GO:0005524", "GO:0005525", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_P47270", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P47270" ], "product": "Ribosome-binding ATPase YchF" }, "genes": [ "ychF" ], "id": "GLJLFKCOHC_162", "locus": "GCA013368035_00120" }, { "type": "cds", "sequence": "contig_1", "start": 79988, "stop": 80185, "strand": "+", "frame": 2, "gene": "ychF", "product": "YchF C-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "nt": "ATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTCGTAAAAGCAGAAGTCATTAAATATGAAGATTATGTTGCTTTAAATGGCGAAAAAGGTTGCAGGGAAGCTGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAA", "aa": "MNAAQCAGIIHTDFEKKFVKAEVIKYEDYVALNGEKGCREAGKIAIEGKNYIMQDGDVCFFRVAK", "aa_hexdigest": "204250049ecabfea5f2f80e5dd4fa1f8", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A380DK80", "query_cov": 0.9538461538461539, "subject_cov": 0.8051948051948052, "identity": 0.629, "score": 89.7, "evalue": 2.79e-22, "valid": false, "product": "GTP-binding and nucleic acid-binding protein YchF", "uniref50_id": "UniRef50_A0A382QXD8" }, "pscc": { "uniref50_id": "UniRef50_A0A382QXD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A382QXD8" ], "product": "YchF C-terminal domain-containing protein" }, "genes": [ "ychF" ], "id": "GLJLFKCOHC_163", "locus": "GCA013368035_00121" }, { "type": "cds", "sequence": "contig_1", "start": 80512, "stop": 80955, "strand": "+", "frame": 1, "gene": null, "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L9U4", "UniRef:UniRef90_D1J7J4" ], "nt": "ATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCTCAAGAATCTGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAATTGCTAGTTTTTCTGACCATGATAATTTTTATATTGAAAGTTATTTGAAACGTCTTGAAATAATCAAACAATACAATATTGATATTACATTATGGCCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATAGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAGCAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGA", "aa": "MNLIKIDLHVHSQDSSHTGSNISQESDLEKLLILTKKRVKIASFSDHDNFYIESYLKRLEIIKQYNIDITLWPGIEVNLRKYDGQIGQAIFIFDPDSNLKELNEVTKKEFRFNSNKYTYKDAVELFKKSNFNFMVFPHAGKGQDNME", "aa_hexdigest": "b0dfdfd3e42bf9c6347136c928fc017a", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 80512, "stop": 81204, "observations": { "mutation": [ 80953, 81079 ], "stop": [ 80953, 81079 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J4", "query_cov": 1.0, "subject_cov": 0.6391304347826087, "identity": 1.0, "score": 295.0, "evalue": 3.3e-100, "gene_start": 1, "gene_end": 147, "reference_start": 1, "reference_end": 147, "reference_sequence": "MNLIKIDLHVHSQDSSHTGSNISQESDLEKLLILTKKRVKIASFSDHDNFYIESYLKRLEIIKQYNIDITLWPGIEVNLRKYDGQIGQAIFIFDPDSNLKELNEVTKKEFRFNSNKYTYKDAVELFKKSNFNFMVFPHAGKGQDNMEWQDICNSQVDGLDITDFNNSNKKKILKQDPNIPVLYFSDTHTWKKYPQYGKFCSYVEVENKDQITFNEVKNNIRNNKVDYDKV" }, "paralog": false, "identity": 0.991304347826087, "score": 464.0, "evalue": 3.38e-169, "description": "Internal stop codon at 80953, 81079. Nonsense mutation around 80953, 81079.", "psc": { "uniref90_id": "UniRef90_D1J7J4", "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A077L9U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J4", "UniRef:UniRef50_A0A077L9U4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L9U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L9U4" ], "product": "Polymerase/histidinol phosphatase N-terminal domain-containing protein" } }, "id": "GLJLFKCOHC_164", "locus": "GCA013368035_00122" }, { "type": "cds", "sequence": "contig_1", "start": 81216, "stop": 81383, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13476.12" ], "nt": "ATGATAAAAAGCCTAAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAGTCAAGTTAATGTTATTGTTGGTGAAAAGGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAATAAAAAAATATTCAAAAACGAATATGAATGA", "aa": "MIKSLKIKNNNETYDINLNSQVNVIVGEKGSGKSTLLLILAEAIVNKKIFKNEYE", "aa_hexdigest": "f601bb4d776aca617f89bc998876efef", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13476.12", "name": "AAA_23", "length": 28, "aa_cov": 0.509090909090909, "hmm_cov": 0.9285714285714286, "evalue": 6.208178973300504e-08, "score": 28.894554138183594, "start": 13, "stop": 40 } ], "seq_stats": { "molecular_weight": 6181.116799999998, "isoelectric_point": 9.050087928771973 }, "id": "GLJLFKCOHC_165", "locus": "GCA013368035_00123" }, { "type": "cds", "sequence": "contig_1", "start": 81654, "stop": 82247, "strand": "+", "frame": 3, "gene": null, "product": "AAA family ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C", "UniRef:UniRef90_UPI001E491A3C" ], "nt": "ATGTTAAAACAATTAGACGATTTTAAATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGACAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAGAGAAAAAATAAATAGTACAAATTTGGTTGTGAATAATCTTTTGTCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTGGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTCATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAAGATAAAAAAACTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTGAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGACAATATAATTCAATAATTGAAAAAGAAATCATTTTAAACTGA", "aa": "MLKQLDDFKLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETREKINSTNLVVNNLLSQFEQSSSLLQISNEQLLDIQQRTENVILQNNSFLEYYNQYNLVVSKDKKTLECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILN", "aa_hexdigest": "e24641f5b8300a1675f55bf484ff2fcd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 81354, "stop": 82274, "observations": { "mutation": [ 81381, 82245 ], "stop": [ 81381, 82245 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491A3C", "query_cov": 1.0, "subject_cov": 0.5549295774647888, "identity": 0.97, "score": 353.0, "evalue": 3.75e-120, "gene_start": 1, "gene_end": 197, "reference_start": 147, "reference_end": 343, "reference_sequence": "MIKSLKIKNNNETYDINLNSQVNVIVGEKGSGKSTLLLILAEAIVNKKLFKNEYEWFNEKAKFLVDSINIDNTEIKTQDFSYIFDSENKQNSKDAGILEIQKNLSGYISQNDSRKNSLDSSEYVEKQKSNCIDNFTEKIYYDQDQKMLKQLDDFRLLEDLLNEYRRERDRQIALSNVFNQSFNLNEGSTKNIATIDYDSSNEIKKIKELLKSIEETKEKIYSTNLVVNNLLAQFEQSSSLLQISNEQLLDIQQRTENVILQNNLFLEYYNQYNLVVSKDKKALECFKLSFTNAKRAYLEELSNTLKIQNDNEQLLEHFSNLGRIMKKINRQYNSIIEKEIILNWTLEETDKKIRI" }, "paralog": false, "identity": 0.9705882352941176, "score": 578.0, "evalue": 2.79e-211, "description": "Internal stop codon at 81381, 82245. Nonsense mutation around 81381, 82245.", "psc": { "uniref90_id": "UniRef90_UPI001E491A3C", "product": "AAA family ATPase", "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491A3C", "UniRef:UniRef50_UPI001E491A3C" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491A3C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491A3C" ], "product": "AAA family ATPase" } }, "id": "GLJLFKCOHC_166", "locus": "GCA013368035_00124" }, { "type": "cds", "sequence": "contig_1", "start": 82425, "stop": 82895, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D", "UniRef:UniRef90_UPI001E54A28D" ], "nt": "ATGATTAAGACATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGATACATTGAACAATGTTCCTAATGAACAATACTTATTGTTAGACCAAATTGAAGATAATCTAGACAATAAAACAATATATGAAAGAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGGCAAACAAATTTTTATCGTTACACATAACCCTAATATTGGAACATTGATAAAAGGCAATATAATAACAACAGATATTTTTAATACTAATTTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACTAAAGGAGATAAATAA", "aa": "MIKTLIKDKIKIYAGDEEYKNLSMGQKTLFGITHAIDTLNNVPNEQYLLLDQIEDNLDNKTIYERIVPMLQEQIKKGKQIFIVTHNPNIGTLIKGNIITTDIFNTNLNKKFVVNEIIREDNEIDTPQSLYLEGSMKALYERLKVHEEKINKTKGDK", "aa_hexdigest": "e4b0239d07094b9124fa2f588bb2fd08", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E54A28D", "query_cov": 1.0, "subject_cov": 0.8387096774193549, "identity": 0.987, "score": 302.0, "evalue": 1.9e-103, "valid": true, "product": "ATP-binding protein", "uniref50_id": "UniRef50_UPI001E54A28D" }, "pscc": { "uniref50_id": "UniRef50_UPI001E54A28D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E54A28D" ], "product": "ATP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_167", "locus": "GCA013368035_00125" }, { "type": "cds", "sequence": "contig_1", "start": 82895, "stop": 83017, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6", "UniRef:UniRef90_D1J7J6" ], "nt": "ATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGA", "aa": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDA", "aa_hexdigest": "0b5c43c96b7a5c397768c5edef3831e1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 82895, "stop": 83191, "observations": { "mutation": [ 83015 ], "stop": [ 83015 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J6", "query_cov": 1.0, "subject_cov": 0.40816326530612246, "identity": 1.0, "score": 78.2, "evalue": 6.29e-18, "gene_start": 1, "gene_end": 40, "reference_start": 1, "reference_end": 40, "reference_sequence": "MITIDYVFTKDEKRLIVISNASDSKNKYKIEIDLDNPSDAWNKENINNFIIRAISISDEKLSEPQLTESAQEQLQKGNKQIEFIKNLFTNFVERYNEN" }, "paralog": false, "identity": 0.9897959183673469, "score": 190.0, "evalue": 1.32e-64, "description": "Internal stop codon at 83015. Nonsense mutation around 83015.", "psc": { "uniref90_id": "UniRef90_D1J7J6", "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J6", "UniRef:UniRef50_D1J7J6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7J6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 4604.149700000001, "isoelectric_point": 4.663718986511231 }, "id": "GLJLFKCOHC_168", "locus": "GCA013368035_00126" }, { "type": "cds", "sequence": "contig_1", "start": 83298, "stop": 83822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05746.21" ], "nt": "ATGAAGAATGGTAAAGAAGTTAAAATGTCAAAACGTTTAGGAACTTCTTTAACGTTAAGAGAACTAGCAGAAGAAGTTGGAAATGATGCAATTAGATACTTTCTAATTGATAGATCATATAATTCAAGAATTGACTTTGATATTAACAAAGTTACTGACAACAATGAAAATAACCCTATGTATATCATTAAATATGCTCATGCTCGTGCTTGCCAATTATTGGATAAAGTAGGAATTAAAAACCCTATTGCTTCTGATGAGTTGGATAATGAATATGCTCAAAAGCTTGTTGCAGAATTAAAAGAATACCCTGAATTAATTTCAACAATGGCCAAAACTTATAAAGTAAACTTGTTGCCTCCTTATCTATTAAAATTATCAAAGGCATTTAACTCATTTTATTCAAATACCAAAGTTTTAGGATCTCCTAACGAACAAAGCCTTGCAGCCTTAGTAAAGGCAACAAAAATTGTTTTAGCTGATGGTTTAAAATTAATGGACATTGAAGCACCTGAAAAAATGTAA", "aa": "MKNGKEVKMSKRLGTSLTLRELAEEVGNDAIRYFLIDRSYNSRIDFDINKVTDNNENNPMYIIKYAHARACQLLDKVGIKNPIASDELDNEYAQKLVAELKEYPELISTMAKTYKVNLLPPYLLKLSKAFNSFYSNTKVLGSPNEQSLAALVKATKIVLADGLKLMDIEAPEKM", "aa_hexdigest": "2f63093e0602344b82c0cb100b2fbede", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05746.21", "name": "DALR_1", "length": 117, "aa_cov": 0.6436781609195402, "hmm_cov": 1.0, "evalue": 1.2389576988878466e-29, "score": 98.16094970703125, "start": 63, "stop": 174 } ], "seq_stats": { "molecular_weight": 19666.535499999998, "isoelectric_point": 8.472322273254395 }, "id": "GLJLFKCOHC_169", "locus": "GCA013368035_00127" }, { "type": "cds", "sequence": "contig_1", "start": 84129, "stop": 84563, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTGCTAACCCAACAGGATATTTGCATATTGGACATGCAAGAAATGCTGCTATTGGTGCAACATTGTGCAATATTCTAGAAAAAGCAGGTCACCGCGTTGTTCGAGAATATTTAGTTAATGATTATGGAAACCAAATGAATAAAATGGCAGATTCAATATTTGCTAGATACCAACAAATATTTAATCCTGAATTTCCTATGCCAGAAGATTCATACCATGGCGGAGATATGATAGAATTCGCTCAAGCATTTTATGACATTCACAAAGATGAATACAAAAATGTAGAATATACTGATGAAATTAGAAAAATCTTTAGAAGTTTTGGAAGAGATTTTGCTTTAAAAAATATTATTGAAGATATGAAAAAATTTGGAGTAACATTCGATATTTTCACATCAGAGGCAGAACAATATGCCAAAAATAGAGTATGA", "aa": "MSANPTGYLHIGHARNAAIGATLCNILEKAGHRVVREYLVNDYGNQMNKMADSIFARYQQIFNPEFPMPEDSYHGGDMIEFAQAFYDIHKDEYKNVEYTDEIRKIFRSFGRDFALKNIIEDMKKFGVTFDIFTSEAEQYAKNRV", "aa_hexdigest": "413d6417d54599f2187c93b44cd613f0", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16722.643999999993, "isoelectric_point": 5.709272193908691 }, "id": "GLJLFKCOHC_170", "locus": "GCA013368035_00128" }, { "type": "cds", "sequence": "contig_1", "start": 84669, "stop": 84923, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03485.21" ], "nt": "ATGAAAACAACGAAAGACATTATTATTGCTTCATTAAATGAAGCACTTGAAAAATTAAATATTTCTAAGCCTATTGTACTTACCGAATCTAAAAATTACGGCGACTATTCAAGCAATATTGCCCTAACATTGCAAAAGGATTTAGGTAAGAAGGCAATAGATATTGCCCAAGAAATAGTAAGAAATATTGATTTAAATAAATTTAAAGAAATTAGTGAAATAAAGGTTGCCGAACCTGGTTTTATTAATTTTTGA", "aa": "MKTTKDIIIASLNEALEKLNISKPIVLTESKNYGDYSSNIALTLQKDLGKKAIDIAQEIVRNIDLNKFKEISEIKVAEPGFINF", "aa_hexdigest": "c4fb2651a79feec6cd8afa5b3577dae8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03485.21", "name": "Arg_tRNA_synt_N", "length": 81, "aa_cov": 0.9404761904761905, "hmm_cov": 0.9753086419753086, "evalue": 8.317434728428708e-20, "score": 66.67340087890625, "start": 6, "stop": 84 } ], "seq_stats": { "molecular_weight": 9409.805399999996, "isoelectric_point": 6.232872962951659 }, "id": "GLJLFKCOHC_171", "locus": "GCA013368035_00129" }, { "type": "cds", "sequence": "contig_1", "start": 84984, "stop": 85466, "strand": "+", "frame": 3, "gene": null, "product": "Cysteine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "nt": "ATGAAAAGATATTATTTATGTGGCCCAACTGTTTATAACTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTCACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAAACAGAAAAAGAAATTTCAAGCTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAGATTGTAAGAGTTACTGATTCACTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAATGGCAATGTTTATTTTGACGTTTTAAAATTTAAAGATGTGTATGGAAAAATAAGCAATCAAAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAGCGCTCGCCATATGATTTTGCTTTATGA", "aa": "MKRYYLCGPTVYNYPHIGNLRPIVSFDLMIRAQRQLGEDVYFLHNITDIDDKIIAKAKQENKTEKEISSFYEAYYLNLFKLFSIEMPNKIVRVTDSLNDMYEYIQSLIEKGAAYQVNGNVYFDVLKFKDVYGKISNQKLANLISDSSNEVDKRSPYDFAL", "aa_hexdigest": "53e44d9bdfe39edeeac9ba27ba052aca", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZZN9", "query_cov": 0.8, "subject_cov": 0.9624060150375939, "identity": 0.672, "score": 190.0, "evalue": 1.14e-59, "valid": false, "product": "Cysteine--tRNA ligase", "uniref50_id": "UniRef50_A0A448ZZN9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZN9" ], "product": "Cysteine--tRNA ligase" }, "genes": [], "id": "GLJLFKCOHC_172", "locus": "GCA013368035_00130" }, { "type": "cds", "sequence": "contig_1", "start": 85692, "stop": 86111, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28", "PFAM:PF01406.26" ], "nt": "TTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAAAATTATTCGGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACTGATGAATTACTTGCATCGCTTCAAAAACAAATTAATAATATTGAGGTACATTTTAATAAAAGTCAATTGGAAGATATTGAATATACGTTAGATAAACAAACAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCATTTAAGCAACTTAATGAGCAATTAAAATCACCACTGAATTATGGTAATGCATTTTTTGTATTTAAGGTTTTAGGCTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGA", "aa": "MNYKNQKMSKSIGNIIYAEEFLKNYSADTYKLLMLSTSYSKPINITDELLASLQKQINNIEVHFNKSQLEDIEYTLDKQTIDSILKNVSNLDFSLAFKQLNEQLKSPLNYGNAFFVFKVLGFKLVENKVSNDDKELYRK", "aa_hexdigest": "c63fe77dff7a0ef55b1a9b0c0bd8c520", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 42, "aa_cov": 0.302158273381295, "hmm_cov": 1.0, "evalue": 1.1657069130177278e-07, "score": 26.243270874023438, "start": 4, "stop": 45 }, { "id": "PF01406.26", "name": "tRNA-synt_1e", "length": 51, "aa_cov": 0.3669064748201439, "hmm_cov": 1.0, "evalue": 2.30938379262232e-10, "score": 35.68574142456055, "start": 2, "stop": 52 } ], "seq_stats": { "molecular_weight": 16179.29899999999, "isoelectric_point": 7.800561714172363 }, "id": "GLJLFKCOHC_173", "locus": "GCA013368035_00131" }, { "type": "cds", "sequence": "contig_1", "start": 86189, "stop": 86656, "strand": "+", "frame": 2, "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "db_xrefs": [ "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173", "SO:0001217", "UniRef:UniRef50_C4XFX7", "UniRef:UniRef90_D1J7J9" ], "nt": "ATGAAAAAGATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAAATTTACTTGCAAAAAGGACTTGTTAACAACAATTTAAAATTTAAGAAAGTTGTTTATTTAGATAGAAATAGCCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGTAGTATTTTTAAAGACGGCTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCTCAAAATTTTGGTGCTATTATACGTAGTGCCAATGTGTTTGGCTTTAAACATATAATAATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGCGTTTTAAAAACTAGCTCAGGGGGTTTTAAAAACGTTAAAGTAATTAAAGTTAACAGCTTATTTGATGCAATTGAGGAATTAAAAAACAAAGGTTTTTGA", "aa": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGF", "aa_hexdigest": "1e54981a63b0844ac6a0f58473b6586c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rlmB" ], "truncated": "3-prime", "pseudogene": { "start": 86189, "stop": 86881, "observations": { "mutation": [ 86654 ], "stop": [ 86654 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7J9", "query_cov": 1.0, "subject_cov": 0.6739130434782609, "identity": 1.0, "score": 313.0, "evalue": 6.4e-107, "gene_start": 1, "gene_end": 155, "reference_start": 1, "reference_end": 155, "reference_sequence": "MKKIVFGKNSVLEAIEYEFPIEEIYLQKGLVNNNLKFKKVVYLDRNSLDKLTENGNHQGYAAKLKDFQYHDLGSIFKDGSDKVLILDHIQDPQNFGAIIRSANVFGFKHIIIPKDRACDVTPSVLKTSSGGFKNVKVIKVNSLFDAIEELKNKGFWIYASALNQQAKKLNDAKFNEKIAIIVGNEGSGVSKTLLKHSDEIIYIEQDKESVQSLNVSVATGILLYTVFNKK" }, "paralog": false, "identity": 0.9956521739130435, "score": 449.0, "evalue": 5.63e-163, "description": "Internal stop codon at 86654. Nonsense mutation around 86654.", "psc": { "uniref90_id": "UniRef90_D1J7J9", "gene": "rlmB", "product": "23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB", "uniref50_id": "UniRef50_C4XFX7", "cog_id": "COG0566", "cog_category": "J", "go_ids": [ "GO:0006396", "GO:0008173" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7J9", "UniRef:UniRef50_C4XFX7", "COG:COG0566", "COG:J", "GO:0006396", "GO:0008173" ] }, "pscc": { "uniref50_id": "UniRef50_C4XFX7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XFX7" ], "product": "RNA 2-O ribose methyltransferase substrate binding domain-containing protein" } }, "id": "GLJLFKCOHC_174", "locus": "GCA013368035_00132" }, { "type": "cds", "sequence": "contig_1", "start": 86947, "stop": 87882, "strand": "-", "frame": 2, "gene": "arcC", "product": "Carbamate kinase", "db_xrefs": [ "COG:COG0549", "COG:E", "RefSeq:WP_036439257.1", "SO:0001217", "UniParc:UPI00051365B9", "UniRef:UniRef100_A0A454C933", "UniRef:UniRef50_P78030", "UniRef:UniRef90_D1J7K0" ], "nt": "ATGAGTAGAATTGTTATTGCATTAGGCGGAAACGCTTTAGGAAATAACCCCGAAGAACAAAAAGAACTTGTTAAAGTTCCTGCAGCCAAAATTGCTGAATTAGTAAAAGCAGGACATGAAGTTATTGTCGGACACGGAAACGGACCACAAGTTGGAATGATTTTCAATGGTTTCGTTAGTGCTCACTCAGTTAATCCTAAGTCTCCTTTAGTTCCATTGCCTGAAGCAGGTGCAATGAGCCAAGGATACATTGGATATCATATGGTTAATGCTATTACAAATGCATTTAAAAACGAAGGATTAAAGAACAAAGAAGTTTTATACATTTTAACTCAAACTCTTGTAGATCCAAAAGACCCAGCATTCCAAAATCCTACAAAACCAATTGGCCCATTCTTTGCAACAGAAGCAGAAGCAGTTGCTGCAAACCCAAATAGCACAATTGTTGAAGATGCAGGTAGAGGCTACAGAAAAGTTGTAGCATCTCCAAAACCATTAAACTTTGTTGGTATTAATCAAATTAAAAAAGCTATTGAATCAGGTGCTACCGTTATCGTTGGTGGTGGTGGTGGAATACCTACTGTTGAAGATGAAAATGGCTTTATTAAGGGTGTAGATGGTGTTATTGACAAAGATTTCGCTTTAGCAAAAATGGCTGCTTTAGCAAAAGCTGATTACTTTATTGTTCTAACAGCAGTTGATTATGTTAAAGTAAATTACAACAAGCCAGATCAAAAAGATCTAAAACACGCTACAAAAGCAGAACTTGAAAAATACATTGCTGAAAAACAATTTGCCCCAGGATCAATGTTACCAAAGGTACAAGCAGCTATTAAATTCGTTGAAGAAGGCGGAAAAGCAGCATTTATTGGTGATTTAAAAGATCTTGAAAACATTCTAGCAGAAAAAATCGGTACAAAAGTTACTTTAAATTAA", "aa": "MSRIVIALGGNALGNNPEEQKELVKVPAAKIAELVKAGHEVIVGHGNGPQVGMIFNGFVSAHSVNPKSPLVPLPEAGAMSQGYIGYHMVNAITNAFKNEGLKNKEVLYILTQTLVDPKDPAFQNPTKPIGPFFATEAEAVAANPNSTIVEDAGRGYRKVVASPKPLNFVGINQIKKAIESGATVIVGGGGGIPTVEDENGFIKGVDGVIDKDFALAKMAALAKADYFIVLTAVDYVKVNYNKPDQKDLKHATKAELEKYIAEKQFAPGSMLPKVQAAIKFVEEGGKAAFIGDLKDLENILAEKIGTKVTLN", "aa_hexdigest": "7d5aecb977527eca1dc65444c741f3ea", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00051365B9", "ncbi_nrp_id": "WP_036439257.1", "uniref100_id": "UniRef100_A0A454C933", "db_xrefs": [ "SO:0001217", "UniParc:UPI00051365B9", "RefSeq:WP_036439257.1", "UniRef:UniRef100_A0A454C933" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C933", "uniref90_id": "UniRef90_D1J7K0" }, "psc": { "uniref90_id": "UniRef90_D1J7K0", "gene": "ArcC", "product": "Carbamate kinase", "uniref50_id": "UniRef50_P78030", "cog_id": "COG0549", "cog_category": "E" }, "pscc": { "uniref50_id": "UniRef50_P78030", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P78030" ], "product": "Carbamate kinase-like protein" }, "genes": [ "arcC" ], "id": "GLJLFKCOHC_175", "locus": "GCA013368035_00133" }, { "type": "cds", "sequence": "contig_1", "start": 87884, "stop": 88132, "strand": "-", "frame": 1, "gene": null, "product": "Ornithine carbamoyltransferase chain F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "nt": "ATGGCAATGATCAAAGCAGCAAAAGAAAATGTTATCTTCCTACACTGCTTACCAGCATTCCACGATGACCAAACTCAATTCTCAGCAACTATTAAAGAAACCTTAGGTGCAAAATACCCAGTTGTTTCAACCGGTGCTATGGAAGTTACTGATGAAGTATTCCAATCAAAATACAACAAATCAATCGAACAAGCTGGAAACCGTATGCACACAATTAAGGCTGTTATTCTAGCTACTTTAGGATACTAA", "aa": "MAMIKAAKENVIFLHCLPAFHDDQTQFSATIKETLGAKYPVVSTGAMEVTDEVFQSKYNKSIEQAGNRMHTIKAVILATLGY", "aa_hexdigest": "0e8f710bbe858e2fe23fe497b4f89f6b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A4U9HLN2", "query_cov": 0.9634146341463414, "subject_cov": 0.9080459770114943, "identity": 0.519, "score": 75.1, "evalue": 4.39e-16, "valid": false, "product": "Ornithine carbamoyltransferase chain F", "uniref50_id": "UniRef50_A0A4U9HLN2" }, "pscc": { "uniref50_id": "UniRef50_A0A4U9HLN2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4U9HLN2" ], "product": "Ornithine carbamoyltransferase chain F" }, "genes": [], "id": "GLJLFKCOHC_176", "locus": "GCA013368035_00134" }, { "type": "cds", "sequence": "contig_1", "start": 88346, "stop": 88507, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00185.30" ], "nt": "ATGCTAGCTGACTACTTAACATTTAAAGAACACGTTGGAGACCTAAGAGGAAAGAAACTTGTTTTCTCAGGTGATATTAAAAACAACGTTGCTCGTTCATTAATGATCGGTGCTGCATTCTTTGGTGTTAATATTGTATTATGTGGACCAAAAGCTCAATGA", "aa": "MLADYLTFKEHVGDLRGKKLVFSGDIKNNVARSLMIGAAFFGVNIVLCGPKAQ", "aa_hexdigest": "ec6f9a72f8a919aca53a992059886de7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00185.30", "name": "OTCace", "length": 36, "aa_cov": 0.6792452830188679, "hmm_cov": 1.0, "evalue": 8.27939006161772e-09, "score": 31.086139678955078, "start": 17, "stop": 52 } ], "seq_stats": { "molecular_weight": 5754.7484, "isoelectric_point": 9.428968620300292 }, "id": "GLJLFKCOHC_177", "locus": "GCA013368035_00135" }, { "type": "cds", "sequence": "contig_1", "start": 88541, "stop": 88927, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02729.26" ], "nt": "ATGCCATTAAATTTAAAGGGTCGTAGTTTAGACAGCGCTCTAAACTTTACAACCGAAGAAATTAACTATTTACTAGATTTATCACTAGAACTAAAAAGAGCAAAATTTGCTGGTTTACACACAAATAATCGCCCATTAGTTGGAAAAAACATTGTTATAATGTTCCAAAAAGACTCAACAAGAACTCGTTGTTCATTTGAAGTTGCAGCTGCTGATTTAGGTGCAAGCTGTACATACATTGGCCCAACAGGATCAAACTTTGGTAAAAAAGAATCAGTTATTGACACAGCTAAAGTTTTAGGCCAAATTTATGATGGTATTGAATTCCGTGGATTCAAACAATCAGATGTTGATGCATTAGTAAAATACTCAGGAATTCCTGTATGA", "aa": "MPLNLKGRSLDSALNFTTEEINYLLDLSLELKRAKFAGLHTNNRPLVGKNIVIMFQKDSTRTRCSFEVAAADLGASCTYIGPTGSNFGKKESVIDTAKVLGQIYDGIEFRGFKQSDVDALVKYSGIPV", "aa_hexdigest": "06307b4f1814626b0733c2ae428eb8cc", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BB40E1", "query_cov": 1.0, "subject_cov": 0.8152866242038217, "identity": 0.563, "score": 144.0, "evalue": 8.79e-42, "valid": false, "uniref50_id": "UniRef50_A0A3D1ABF9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02729.26", "name": "OTCace_N", "length": 126, "aa_cov": 0.9375, "hmm_cov": 0.9841269841269841, "evalue": 3.24034270985284e-30, "score": 100.5682373046875, "start": 9, "stop": 128 } ], "seq_stats": { "molecular_weight": 14021.913599999985, "isoelectric_point": 8.562320137023928 }, "id": "GLJLFKCOHC_178", "locus": "GCA013368035_00136" }, { "type": "cds", "sequence": "contig_1", "start": 89121, "stop": 89369, "strand": "-", "frame": 3, "gene": "mscL", "product": "Large-conductance mechanosensitive channel", "db_xrefs": [ "COG:COG1970", "COG:M", "KEGG:K03282", "SO:0001217", "UniRef:UniRef50_D1J7K2", "UniRef:UniRef90_D1J7K2" ], "nt": "TTGGGTGCTTTAATTCAATTTATTATTGTTTCAACATTTATTTTTATAGGATTAATGATTGTATTTACAATTAAGAATTCTATTCTATATGCAAAAGCTAAAAAGAACCCAATTGAAGAAGAAATAAAGGAAGAACCTATTCCAACAACAGAAGAATTAATTTTAAATGAATTAAAAAAATTAAATGAAAATTTATTAGAAACGAAAAATTCTGGGAATAAAAAAGATTTAAAAAATGATTTAAAATAA", "aa": "MGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKNPIEEEIKEEPIPTTEELILNELKKLNENLLETKNSGNKKDLKNDLK", "aa_hexdigest": "2403515ac7873ccf41c2d062b2d5d31e", "start_type": "TTG", "rbs_motif": null, "genes": [ "mscL" ], "truncated": "3-prime", "pseudogene": { "start": 89121, "stop": 89669, "observations": { "mutation": [ 89423, 89432 ], "stop": [ 89423, 89432 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K2", "query_cov": 1.0, "subject_cov": 0.4293193717277487, "identity": 0.9390000000000001, "score": 135.0, "evalue": 1.18e-38, "gene_start": 1, "gene_end": 82, "reference_start": 110, "reference_end": 191, "reference_sequence": "FLLIKKTKTRIKFILYNIDMTSKELEEKKHYIKKSYVDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAIGMKNLDAWVVWPGIHATKETGGIFIGKFLGALIQFIIVSTFIFIGLMIVFTIKNSILYAKAKKSPIEEEIKEEPTPTTEELILNELKKLNENLLETKNSGDKKDLKNNLK" }, "paralog": false, "identity": 0.9505494505494505, "score": 332.0, "evalue": 8.02e-119, "description": "Internal stop codon at 89423, 89432. Nonsense mutation around 89423, 89432.", "psc": { "uniref90_id": "UniRef90_D1J7K2", "gene": "MscL", "product": "Large-conductance mechanosensitive channel", "uniref50_id": "UniRef50_D1J7K2", "cog_id": "COG1970", "cog_category": "M", "kegg_orthology_id": "K03282", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K2", "UniRef:UniRef50_D1J7K2", "COG:COG1970", "COG:M", "KEGG:K03282" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K2" ], "product": "Large-conductance mechanosensitive channel" } }, "id": "GLJLFKCOHC_179", "locus": "GCA013368035_00137" }, { "type": "cds", "sequence": "contig_1", "start": 89430, "stop": 89639, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01741.23" ], "nt": "ATGACAAGTAAAGAATTAGAAGAAAAAAAGCATTATATTAAAAAGTCATATATAGATGCAAAAAAAATAGTTTCGCGTGGAAACATGTTCATGCTAGCAATTGGGCTTTTATTAGGAGCTTCATTTGGGGCACTAGTATCATCACTAGCAAATGATGTTATTATGTCAGCAATCACAAAGGCAGTCGGTATGAAAAATCTTGATGCTTGA", "aa": "MTSKELEEKKHYIKKSYIDAKKIVSRGNMFMLAIGLLLGASFGALVSSLANDVIMSAITKAVGMKNLDA", "aa_hexdigest": "f66c04029516805ebdcad7631d08d727", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01741.23", "name": "MscL", "length": 47, "aa_cov": 0.6811594202898551, "hmm_cov": 1.0, "evalue": 6.144025470902686e-13, "score": 44.62319564819336, "start": 20, "stop": 66 } ], "seq_stats": { "molecular_weight": 7462.8359999999975, "isoelectric_point": 9.573893547058105 }, "id": "GLJLFKCOHC_180", "locus": "GCA013368035_00138" }, { "type": "tRNA", "sequence": "contig_1", "start": 89710, "stop": 89802, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(gct)", "amino_acid": "Ser", "anti_codon": "gct", "score": 68.9, "nt": "GGGTAAGTACTCAAGTGGTCGAAGAGGCGGTCCTGCTAAGACTGTAGGGGTGCTAAAACACCCGCGGAGGTTCAAATCCTCTCTTACCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 89766, 89768 ], "id": "GLJLFKCOHC_33", "locus": "GCA013368035_00139" }, { "type": "cds", "sequence": "contig_1", "start": 89870, "stop": 90037, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATTATATTAGTACAAATCCTCAAAGCATTTATTACACAAAGAACATTGAATACTACAAACAATTACAAGAAGCTTCTAATCATGCTAAAAATTTAAAATTAGGAATTTTTGATAGTGGCAATGATATCAATAAAATTTACCCTCAAAGATCAAGGAAAAATTAA", "aa": "MYYISTNPQSIYYTKNIEYYKQLQEASNHAKNLKLGIFDSGNDINKIYPQRSRKN", "aa_hexdigest": "b48206481d340a12df4c8d8f6a79488f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6563.281300000002, "isoelectric_point": 9.476675224304202 }, "id": "GLJLFKCOHC_181", "locus": "GCA013368035_00140" }, { "type": "cds", "sequence": "contig_1", "start": 90194, "stop": 90550, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCGTCACGAATAAAAAAACTTACTAGGATAGCTAAGTCGAATAATCAAAACGACACAAATAATGACAAATTTAACATCAATAATTTTCATGAAGCTAAATTTGATTTTGAAATACTAAAAAATAATAAATTTGAAGTAGGAAAAGCAACAGAAATAAATATTGCAAGAATTGCTGATGGTGATACAATCTCAGATTCCTATCGAAATAAATATCGCTTTAATGGAATTGATACACCAGAAACTCATAAAATAACTAATGGAAAATTTGAAGAAACATCTGGCATTCAATATAAATATGGAAAAATTGCCGAAAATTATACAAAGAATTTTCTAAACAATGCTGCTAAAATTTGA", "aa": "MSSRIKKLTRIAKSNNQNDTNNDKFNINNFHEAKFDFEILKNNKFEVGKATEINIARIADGDTISDSYRNKYRFNGIDTPETHKITNGKFEETSGIQYKYGKIAENYTKNFLNNAAKI", "aa_hexdigest": "ca3150af2e04c75704f0a9d4e939635a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13618.985999999979, "isoelectric_point": 9.396927833557129 }, "id": "GLJLFKCOHC_182", "locus": "GCA013368035_00141" }, { "type": "cds", "sequence": "contig_1", "start": 90569, "stop": 90823, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAGAAAAACTAAAAAAATATTGAATCTTGCATTGAGCTCTATTGCTACAATTTTGCCAATTTCAATAGTTTCATATTCTTGCAAACTAGAAAAAATTAATGACAAGGAACAACAAAATTTTAAAGAAAAATATGACAAAAAAATAAAGGAAATTGAAAATAGCAACGAATGGCAAAAAAATGATTATTATAAACAATTAATTGCTGATGCCAAAAAATTAATAAATTCAAACAATAAAAAAATTGATTGA", "aa": "MERKTKKILNLALSSIATILPISIVSYSCKLEKINDKEQQNFKEKYDKKIKEIENSNEWQKNDYYKQLIADAKKLINSNNKKID", "aa_hexdigest": "09c945423988031408f4c739627176cd", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9921.364199999989, "isoelectric_point": 9.40846767425537 }, "id": "GLJLFKCOHC_183", "locus": "GCA013368035_00142" }, { "type": "cds", "sequence": "contig_1", "start": 91110, "stop": 91499, "strand": "-", "frame": 3, "gene": "fba", "product": "Fructose-bisphosphate aldolase", "db_xrefs": [ "COG:COG0191", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7", "UniRef:UniRef90_D1J7K4" ], "nt": "ATGGGTGGCTATAAAACCGTTGTCAATTTGGTTAATGGTCTTATTCAAGATTTAGAAATTAAAACTAATGTCATATTGCACTTAGATCATGGTAATTATAACGATTGTATTAATGCTATAAATGCCGGCTTTAAGTCAATAATGTATGATGGTAGCAAAGAAGATTTTGAAACCAATATATTGAACACAAAAAAAATAGTTAACATTGCAAAAAAAATGGATATAACTGTTGAAGCAGAAGTAGGTAGAATTGGACTAGATATTAGTAAAAATAAATCACTTGATATTCAAAAAACAAACATTGAAGATGCTAAAAAATTAGCAAATACTGGAATTGATTTATTAGCAGTGGCAATTGGAAATATTCATGGTCAATATGAAAGTAATTGA", "aa": "MGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESN", "aa_hexdigest": "62dcf696b3b57efd0a16fdc9dc206a63", "start_type": "ATG", "rbs_motif": null, "genes": [ "fba" ], "truncated": "both", "pseudogene": { "start": 90807, "stop": 91691, "observations": { "mutation": [ 91112, 91580 ], "stop": [ 91112, 91580 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K4", "query_cov": 1.0, "subject_cov": 0.4387755102040816, "identity": 1.0, "score": 254.0, "evalue": 3.48e-83, "gene_start": 1, "gene_end": 129, "reference_start": 65, "reference_end": 193, "reference_sequence": "MIKLKRKNMEIQNLNQTLKKIKEENYAILHININNLEWAKFVLLATKQANLDIILGASPSAIKYMGGYKTVVNLVNGLIQDLEIKTNVILHLDHGNYNDCINAINAGFKSIMYDGSKEDFETNILNTKKIVNIAKKMDITVEAEVGRIGLDISKNKSLDIQKTNIEDAKKLANTGIDLLAVAIGNIHGQYESNWKGLDFELLDKLNQEIKIPLVLHGASGIKEDDIKKAIRLGIKKINFNTELQIANAKALNDFLKNNNFLENKNYNPRILYANSNQAIYKCTLDLLNKYGKKN" }, "paralog": false, "identity": 0.9897959183673469, "score": 569.0, "evalue": 6.43e-210, "description": "Internal stop codon at 91112, 91580. Nonsense mutation around 91112, 91580.", "psc": { "uniref90_id": "UniRef90_D1J7K4", "gene": "Fba", "product": "Fructose-bisphosphate aldolase", "uniref50_id": "UniRef50_A0A3B0NXB7", "cog_id": "COG0191", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K4", "UniRef:UniRef50_A0A3B0NXB7", "COG:COG0191", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NXB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NXB7" ], "product": "Fructose-bisphosphate aldolase" } }, "id": "GLJLFKCOHC_184", "locus": "GCA013368035_00143" }, { "type": "cds", "sequence": "contig_1", "start": 91888, "stop": 91998, "strand": "-", "frame": 2, "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "db_xrefs": [ "EC:2.7.7.6", "GO:0003899", "GO:0006351", "SO:0001217", "UniRef:UniRef50_N9VBR1", "UniRef:UniRef90_D1J7K5" ], "nt": "ATGGAATATAAATCGCAAGTTGATATAGCCAAAGAAATTTTAGATAGACACGATGCACTAGATTTTGAAACTTTATTTAATTATGTAAAAGACGAACTACTTGAACGTTGA", "aa": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLER", "aa_hexdigest": "c8d54175b4f84f2b8947c9e2089e7878", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rpoE" ], "truncated": "5-prime", "pseudogene": { "start": 91741, "stop": 91998, "observations": { "mutation": [ 91773, 91890 ], "stop": [ 91773, 91890 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K5", "query_cov": 1.0, "subject_cov": 0.4235294117647059, "identity": 1.0, "score": 72.8, "evalue": 5.06e-16, "gene_start": 1, "gene_end": 36, "reference_start": 1, "reference_end": 36, "reference_sequence": "MEYKSQVDIAKEILDRHDALDFETLFNYVKDELLERWKEELTSSISDEELLIKKRGELYKLLTIDGRFFKKVNGLWTSKRPEFKN" }, "paralog": false, "identity": 0.9764705882352941, "score": 162.0, "evalue": 8.98e-54, "description": "Internal stop codon at 91773, 91890. Nonsense mutation around 91773, 91890.", "psc": { "uniref90_id": "UniRef90_D1J7K5", "gene": "rpoE", "product": "DNA-directed RNA polymerase subunit delta", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_N9VBR1", "go_ids": [ "GO:0003899", "GO:0006351" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K5", "EC:2.7.7.6", "UniRef:UniRef50_N9VBR1", "GO:0003899", "GO:0006351" ] }, "pscc": { "uniref50_id": "UniRef50_N9VBR1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9VBR1" ], "product": "HTH HARE-type domain-containing protein" } }, "id": "GLJLFKCOHC_185", "locus": "GCA013368035_00144" }, { "type": "cds", "sequence": "contig_1", "start": 92448, "stop": 93050, "strand": "+", "frame": 3, "gene": null, "product": "arginine deiminase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "nt": "ATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTTTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGA", "aa": "MSVFDSKFNGIHVYSEIGELETVLVHEPGREIDYITPARLDELLFSAILESHDARKEHQSFVKIMKDRGINVVELTDLVAETYDLASKAAKEEFIETFLEETVPVLTEANKKAVRAFLLSKPTHEMVEFMMSGITKYELGVESENELIVDPMPNLYFTRDPFASVGNGVTIHFMRYIVRRRETLFARFVFRNHPKLVKTP", "aa_hexdigest": "a1b2024decd2b61298954f3300560145", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0005698E2F", "query_cov": 0.915, "subject_cov": 0.9104477611940298, "identity": 0.574, "score": 216.0, "evalue": 3.11e-68, "valid": false, "product": "arginine deiminase family protein", "uniref50_id": "UniRef50_UPI0005698E2F" }, "pscc": { "uniref50_id": "UniRef50_UPI0005698E2F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005698E2F" ], "product": "arginine deiminase family protein" }, "genes": [], "id": "GLJLFKCOHC_186", "locus": "GCA013368035_00145" }, { "type": "cds", "sequence": "contig_1", "start": 93072, "stop": 93236, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATTAATGTTCCTAAATGA", "aa": "MSIEGGDVFIYNNETLVVGVSERTDLDTITLLAKNIKANKDVEFKRIVAINVPK", "aa_hexdigest": "72de146cd63d2994b08b2ca4ff486d08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5992.849600000001, "isoelectric_point": 5.135198402404785 }, "id": "GLJLFKCOHC_187", "locus": "GCA013368035_00146" }, { "type": "cds", "sequence": "contig_1", "start": 93381, "stop": 93674, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTTGACGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTACGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTGCCATTCCACGGAAACCAACTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGA", "aa": "MPLDKLLASIINKEPVLIPIGGAGATEMEIARETNFDGTNYLAIKPGLVIGYDRNEKTNAALKAAGITVLPFHGNQLSLGMGNARCMSMPLSRKDVK", "aa_hexdigest": "7c5eac652468c5e656c36d6582d9a4e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10354.017699999995, "isoelectric_point": 8.851718711853028 }, "id": "GLJLFKCOHC_188", "locus": "GCA013368035_00147" }, { "type": "cds", "sequence": "contig_1", "start": 93791, "stop": 94087, "strand": "-", "frame": 1, "gene": "dut2", "product": "dUTP diphosphatase", "db_xrefs": [ "COG:COG4508", "COG:F", "SO:0001217", "UniRef:UniRef50_D1J7K7", "UniRef:UniRef90_D1J7K7" ], "nt": "ATGTTAGAAGAATATGCAGATGGAATTCATTTTATTACTAGTATCGCATTAAAAAATAACATAACCTCATTTGATATAAAGCCAATAATTAAATATAATGACTTTACCTTGCAACTTGCTTATACATATCAATGCTTTACCAAATTGTTTTTGCACCATAATAAAAGATTAATAAAAAAAGCATATGGTGTTTATTTGGGATTAGGTGAAATTATGAATATTGATCGCGATATGATTTGCACGGCCTATGCCAAAAAAAATCATAAAAACTATGAAAGAATAAAAAATAATTATTAA", "aa": "MLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY", "aa_hexdigest": "9131402a92497820c79d2cde424a1510", "start_type": "ATG", "rbs_motif": null, "genes": [ "dut2" ], "truncated": "3-prime", "pseudogene": { "start": 93791, "stop": 94318, "observations": { "mutation": [ 94120 ], "stop": [ 94120 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7K7", "query_cov": 1.0, "subject_cov": 0.56, "identity": 1.0, "score": 204.0, "evalue": 6.06e-66, "gene_start": 1, "gene_end": 98, "reference_start": 78, "reference_end": 175, "reference_sequence": "MELFLTFKFIIQNMNLTEVFEAQKKLDLAFASSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKYWKKDKSINQISMLEEYADGIHFITSIALKNNITSFDIKPIIKYNDFTLQLAYTYQCFTKLFLHHNKRLIKKAYGVYLGLGEIMNIDRDMICTAYAKKNHKNYERIKNNY" }, "paralog": false, "identity": 0.9885714285714285, "score": 343.0, "evalue": 3.23e-123, "description": "Internal stop codon at 94120. Nonsense mutation around 94120.", "psc": { "uniref90_id": "UniRef90_D1J7K7", "gene": "Dut2", "product": "dUTP diphosphatase", "uniref50_id": "UniRef50_D1J7K7", "cog_id": "COG4508", "cog_category": "F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7K7", "UniRef:UniRef50_D1J7K7", "COG:COG4508", "COG:F" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7K7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7K7" ], "product": "dUTP diphosphatase" } }, "id": "GLJLFKCOHC_189", "locus": "GCA013368035_00148" }, { "type": "cds", "sequence": "contig_1", "start": 94118, "stop": 94318, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08761.16" ], "nt": "ATGGAATTATTTTTAACTTTTAAGTTTATAATTCAAGACATGAATTTAACAGAAGTTTTTGAAGCCCAAAAGAAATTAGACCTTGCATTTGCAAGTAGCATTGAAAAAACAGAATTTTTGAATATCAAAATTAAAATGGTTATTGCTTTGCTTGTTGAACTAGGAGAATTTGCAAACGAAGTAAAACTATTTAAATATTGA", "aa": "MELFLTFKFIIQDMNLTEVFEAQKKLDLAFASSIEKTEFLNIKIKMVIALLVELGEFANEVKLFKY", "aa_hexdigest": "6a712b3bb771e61ab6150dc560d0e1cf", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08761.16", "name": "dUTPase_2", "length": 52, "aa_cov": 0.7878787878787878, "hmm_cov": 1.0, "evalue": 3.7769882947119523e-10, "score": 35.704444885253906, "start": 15, "stop": 66 } ], "seq_stats": { "molecular_weight": 7736.150999999995, "isoelectric_point": 4.9959428787231435 }, "id": "GLJLFKCOHC_190", "locus": "GCA013368035_00149" }, { "type": "tRNA", "sequence": "contig_1", "start": 94353, "stop": 94436, "strand": "+", "gene": "trnL", "product": "tRNA-Leu(caa)", "amino_acid": "Leu", "anti_codon": "caa", "score": 69.5, "nt": "GCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 94386, 94388 ], "id": "GLJLFKCOHC_3", "locus": "GCA013368035_00150" }, { "type": "cds", "sequence": "contig_1", "start": 94673, "stop": 95410, "strand": "+", "frame": 2, "gene": null, "product": "RDD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13", "UniRef:UniRef90_D1J7K8" ], "nt": "TTGCTAATATCTGCAATTGGTTTGCTTGTTTTGATTTATATTACATTAGTCTTTAAAAACAAATATGAAGAATCTTTTGAACTTGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTAGGAATATTTTCAGGAGCATTGTTGGTATTGGCTTCTTTTATATTGCTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAACAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAATTTAGTTAATGAAATTAATAGCGATTTTGCTAAACATTCATTTTTAGATCCAAATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGATTAATTATCTTTGAAATTGTCATTCCTTTATTTATTTCTATTGCATTAATAGTATTAATTGCATGTCTTTTTAACAATGATGATCAACCAATCAGGGCAATTTATAGATTGATAATCATTTTAGTCAGCGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTGAAAAATTATCAAAATTTCAATAATTTATATTTACTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAG", "aa": "MLISAIGLLVLIYITLVFKNKYEESFELEIFNDIFVSCLVGIFSGALLVLASFILLDVFKRNKIKNFFEYYCYLSSLKSKNKFLFFKNYDLREIINNKNQLTKQEFICFVAEKLNYTMPSIEYKNLVNEINSDFAKHSFLDPNFKKQKKATLTRLIIFEIVIPLFISIALIVLIACLFNNDDQPIRAIYRLIIILVSAIIVLSLSILIYELFILSKVKNYQNFNNLYLLSFNNYSYKYLNSSLVR", "aa_hexdigest": "f8a2244f6ecfc16589463471aa006266", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7K8", "query_cov": 1.0, "subject_cov": 0.9074074074074074, "identity": 0.988, "score": 444.0, "evalue": 1.61e-156, "valid": true, "uniref50_id": "UniRef50_N9TR13" }, "pscc": { "uniref50_id": "UniRef50_N9TR13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TR13" ], "product": "RDD domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_191", "locus": "GCA013368035_00151" }, { "type": "cds", "sequence": "contig_1", "start": 95773, "stop": 95994, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCCGCATCAGGACTTTCTGCTGCTTGTGGTCATACGGGTGGCGGTTCAGGAACTAGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCTTCAGGATTTTCTAACACAAATAAACAAGGTATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGA", "aa": "MKKINKILLGLAPAAALIAASGLSAACGHTGGGSGTSYGFDQTDDGKLVLASGFSNTNKQGIALKAVVDAYNK", "aa_hexdigest": "3afdec348b509f7875ec70c269bfb2cf", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7240.189900000001, "isoelectric_point": 9.25226078033447 }, "id": "GLJLFKCOHC_192", "locus": "GCA013368035_00152" }, { "type": "cds", "sequence": "contig_1", "start": 96049, "stop": 96273, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTAATGGATATAATACCAATACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCCATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAATGGATTTAATTTGGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGA", "aa": "MANGYNTNTLRSDLDAKNSNDFYNMTLNYPAAASILSQYKMNLAISKEDYDKFGIENGFNLGNDLIGGNTKKEK", "aa_hexdigest": "38631733507daf8f03dc1e3ab2f14276", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8248.035399999997, "isoelectric_point": 5.125819969177246 }, "id": "GLJLFKCOHC_193", "locus": "GCA013368035_00153" }, { "type": "cds", "sequence": "contig_1", "start": 96301, "stop": 96456, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTCAGTTGCTAAAGCATTAGTAGGAAAATTCATTAAAGAATTAAACGGCTTGGGTGTAAAAATTTCTGACACAGACAATACTAAAGTTAAGGAATATCTAGACTATTACACAACAAATAAAAATGAAGCACAAGGTGTTGATGAAACTTGA", "aa": "MLSVAKALVGKFIKELNGLGVKISDTDNTKVKEYLDYYTTNKNEAQGVDET", "aa_hexdigest": "1aff45f4de32d6629eee1020cae1fec1", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5653.327200000002, "isoelectric_point": 5.135198402404785 }, "id": "GLJLFKCOHC_194", "locus": "GCA013368035_00154" }, { "type": "cds", "sequence": "contig_1", "start": 96520, "stop": 96831, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATCAAATTTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACCAATGGAGATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGTGGATTTGACTACTCAATATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAAGATAGTTGACTTAATCCTAGAAGGTGTTAAAGAAGGCGCTGTTTGA", "aa": "MSDQIFRDYSSMINFSIQAKKMYNKDPLLNVIGFDSLPNVINTMTTSLTNGDINKGYIKPDEDKITTGGFDYSIFLKDANSEQSKLFKKIVDLILEGVKEGAV", "aa_hexdigest": "6eeb5adbd35c2d67c22e9aea3037f275", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11549.06119999999, "isoelectric_point": 4.977186012268065 }, "id": "GLJLFKCOHC_195", "locus": "GCA013368035_00155" }, { "type": "cds", "sequence": "contig_1", "start": 96886, "stop": 97032, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCCTTGGATCAACTGCCGGATACTCACATACATTCTCAACTGCTAAATCATTAACAAACTTAAAAATAAAAGATACAACTACTTCATTAGATCAATCATCATTGGAAGTTTTAGGCCAAAAACCTGGTGAATCAGATAAATGA", "aa": "MSLGSTAGYSHTFSTAKSLTNLKIKDTTTSLDQSSLEVLGQKPGESDK", "aa_hexdigest": "3eeeff7617bcc3c7fcaa5c172eaf3da8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5061.543700000001, "isoelectric_point": 6.521330833435059 }, "id": "GLJLFKCOHC_196", "locus": "GCA013368035_00156" }, { "type": "cds", "sequence": "contig_1", "start": 97495, "stop": 97884, "strand": "+", "frame": 1, "gene": null, "product": "Putative mycoplasma lipoprotein, C-terminal region", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230", "UniRef:UniRef90_UPI00215C1230" ], "nt": "ATGTTCCAACACGATTTAGAAAACTTTGAAATTGTTGAAGTTTCTAAAAAAGGAGAAACTAAAACAACATTTGACGGTAAGCAAAAACCTATTGATATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGGTAAAGCAAGCAAATTAAATAAAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTACATTCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCGTTATCAAAAGCAAACGTTAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAA", "aa": "MFQHDLENFEIVEVSKKGETKTTFDGKQKPIDIFNEYGSYISPTKSYFSSGKASKLNKATKIAFDNFKQLDNKDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL", "aa_hexdigest": "7e204d0ce4d4564c81ea7be4ff6fc2f4", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 97330, "stop": 97884, "observations": { "mutation": [ 97369, 97492 ], "stop": [ 97369, 97492 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C1230", "query_cov": 1.0, "subject_cov": 0.6972972972972973, "identity": 0.907, "score": 222.0, "evalue": 2.98e-72, "gene_start": 1, "gene_end": 129, "reference_start": 57, "reference_end": 185, "reference_sequence": "MVSSNKILNEADADWISSPLAFDKNAKKAVFIQGPSILPIHANEKEDKATKLFINWMFQHDLENFKIEEVFKGKTTTKTFEGKQKPIDIFNEYGSYISPTKSYFSSDKASKLNKATKIAFDNFKQLDKEDSGYIPSEDIATSLSDVLREAITSAGKAALSKANVNEVFEYKEFVSKILGQLSNQL" }, "paralog": false, "identity": 0.9130434782608695, "score": 321.0, "evalue": 9.47e-114, "description": "Internal stop codon at 97369, 97492. Nonsense mutation around 97369, 97492.", "psc": { "uniref90_id": "UniRef90_UPI00215C1230", "product": "Putative mycoplasma lipoprotein, C-terminal region", "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C1230", "UniRef:UniRef50_UPI00215C1230" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215C1230", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215C1230" ] } }, "id": "GLJLFKCOHC_197", "locus": "GCA013368035_00157" }, { "type": "cds", "sequence": "contig_1", "start": 97972, "stop": 98511, "strand": "+", "frame": 1, "gene": "yncB", "product": "TNase-like domain-containing protein", "db_xrefs": [ "COG:COG1525", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7L0", "UniRef:UniRef90_D1J7L0" ], "nt": "ATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGCTCCGTTACTGTCCGTATCATGCAAGACAGTGGATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATAAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATAAAAAAGGAAATAAAACTATTAAAGAAGTTTCTCAATCATTATGGGATTATTATAATTACATTGAAGGCGAAGTTACTAGAATTTTTGATGGTGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAATGTTCCTACAACAATGAAATTAAGAATTCCAATGATTGATACATTAGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGA", "aa": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYKFSKVGTIFNKSINYRMYVTDKKGNKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSN", "aa_hexdigest": "e47ab1d48820cd368fe528db1295b6c7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "yncB" ], "truncated": "3-prime", "pseudogene": { "start": 97972, "stop": 98814, "observations": { "mutation": [ 98509 ], "stop": [ 98509 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L0", "query_cov": 1.0, "subject_cov": 0.5559006211180124, "identity": 0.983, "score": 344.0, "evalue": 1.31e-117, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MKKYNKFLLILASSAGLVVGAPLLSVSCKTVDKEKINLSKFDVIKKNYEIPVYEFSKVGTIFNKSINYRMYVTDEKGDKTIKEVSQSLWDYYNYIEGEVTRIFDGDTIEIKVTKQPNPKPGGKVLNVPTTMKLRIPMIDTLEENTPEVGKEEKELAKKDHAYAEKLIPIGSKVRVISSNWSEKTYDRFVGYVFFGKNFERQFDIEMLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNYWKKPKLATKKNNIYRYLEQINQNKK" }, "paralog": false, "identity": 0.9857142857142858, "score": 547.0, "evalue": 6.21e-200, "description": "Internal stop codon at 98509. Nonsense mutation around 98509.", "psc": { "uniref90_id": "UniRef90_D1J7L0", "gene": "YncB", "product": "TNase-like domain-containing protein", "uniref50_id": "UniRef50_D1J7L0", "cog_id": "COG1525", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L0", "UniRef:UniRef50_D1J7L0", "COG:COG1525", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L0" ], "product": "TNase-like domain-containing protein" } }, "id": "GLJLFKCOHC_198", "locus": "GCA013368035_00158" }, { "type": "cds", "sequence": "contig_1", "start": 98587, "stop": 98865, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGCGGGGGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCTGAATTTATTGCTGATTTGTCAAAAGAAAGCACTGAAGCAAAAGAAATAAGGAGCTACTTGTTACCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCTTATGAACTAAGTTCAAAATATTTAGAACACGGACAAGGTATGGTTGATTTCTCACTTTCAATACTTCATTATAACTATTGA", "aa": "MLAGGYTLARLDTTKVISEFIADLSKESTEAKEIRSYLLPYAAYAINYGILNRKGFYGEVANMRSPYELSSKYLEHGQGMVDFSLSILHYNY", "aa_hexdigest": "8df4e3bfd313c4224aaa41d011cbb95d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10429.735599999998, "isoelectric_point": 6.0617876052856445 }, "id": "GLJLFKCOHC_199", "locus": "GCA013368035_00159" }, { "type": "cds", "sequence": "contig_1", "start": 98952, "stop": 99350, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAAGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACGACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGGGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTCCAAAACTATGCACTATATCCTCACATGTCAGTTTATGAAAATATTGCATTTCCTCTATACAATGACAAACATTGA", "aa": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKH", "aa_hexdigest": "8d40a7fa5d1c53b48bb03bab9d1f50ef", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 78, "aa_cov": 0.5757575757575758, "hmm_cov": 1.0, "evalue": 2.497093354771237e-21, "score": 72.1390151977539, "start": 47, "stop": 122 } ], "seq_stats": { "molecular_weight": 14727.77869999998, "isoelectric_point": 8.66733913421631 }, "id": "GLJLFKCOHC_200", "locus": "GCA013368035_00160" }, { "type": "cds", "sequence": "contig_1", "start": 99387, "stop": 100361, "strand": "+", "frame": 3, "gene": "potA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG3842", "COG:E", "SO:0001217", "UniRef:UniRef50_D1J7L1", "UniRef:UniRef90_D1J7L1" ], "nt": "TTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTCGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAAAAGTATTTAAATGAATTAATTTCACAATATAACGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACTTCGCTAACTAAAAATATTTTGCAATATATTAAAAAACTAGAATTGCAACGCCAAGCAGAAATTTCGGAAGCTAGAGAAAAATTTTTGAGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTAAAAAATATATCAGCATTAAAATCAGAATTTTCAGATACTGTTTCTAAAATTAAATCTAAAAGTGCAGATGAGATTGCTAATGCTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAATACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACGCGAACAAGCAGAATCTCCATTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACAAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAGTTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTGGATCAAGAAACTCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGACGAAGCAATAGTAAAAGATGTATTAGATGTAGCGCAAAGAGTTGAAATTACAAAGAACTTAGCAAAACGTCCTACTCAAATGTCTGGTGGTCAACAACAACGTGTTGCCATAGCTAGAGCAATTGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGA", "aa": "MKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNILQYIKKLELQRQAEISEAREKFLSEKQKLDREFEFTHDKNILKNISALKSEFSDTVSKIKSKSADEIANAKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGSFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRK", "aa_hexdigest": "8ee2514f0920c211c91d20ec5b86c757", "start_type": "TTG", "rbs_motif": null, "genes": [ "potA" ], "truncated": "both", "pseudogene": { "start": 99087, "stop": 100664, "observations": { "mutation": [ 99348, 100359 ], "stop": [ 99348, 100359 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L1", "query_cov": 1.0, "subject_cov": 0.500772797527048, "identity": 0.8640000000000001, "score": 423.0, "evalue": 1.12e-141, "gene_start": 1, "gene_end": 324, "reference_start": 146, "reference_end": 469, "reference_sequence": "MKETKSREELLKDIHSFSKQRHEIKKTLPAIEIKNLVIDFGESLAVDNASFCINKGELVTLLGPSGSGKTTTLNAIAGLLRPTGGKIFFSGIDVTKFSPQQRELGLVFQNYALYPHMSVYENIAFPLYNDKHWREETLEKTKLSQLKIFEIILNAYKVDEKAIEDLKTKCYYSIDNPKETKKYLNELISQYNDIIDEQINKLNFYKTKKTAEGTSLTKNVLQYIKKLGLQRQAEISEAREKFLGEKQKLDREFEFTHDKNILKNISVLKSEFSDTVSKIKSKSAEEIANVKKEYSEKYNEALARNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRYVLVSKITNVEYTEAVENVFGSLFNSQNITKGNFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9142857142857143, "score": 830.0, "evalue": 5.44e-304, "description": "Internal stop codon at 99348, 100359. Nonsense mutation around 99348, 100359.", "psc": { "uniref90_id": "UniRef90_D1J7L1", "gene": "PotA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J7L1", "cog_id": "COG3842", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L1", "UniRef:UniRef50_D1J7L1", "COG:COG3842", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7L1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L1" ], "product": "ABC transporter ATP-binding protein" } }, "id": "GLJLFKCOHC_201", "locus": "GCA013368035_00161" }, { "type": "cds", "sequence": "contig_1", "start": 100428, "stop": 100892, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6", "UniRef:UniRef90_UPI00215C93E5" ], "nt": "ATGTCAATTAGCGACAAGATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCTCAAATTAAAGACGGATATATTATTTACAACAATAAAAAACTACTTAAAGCTCCTGAGGGATATCAAAAATCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAGGAAGAAGAAGGAATATTTGAAGGAAGCATAAAATTAGTTGAACATTTAGGTAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTACGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAA", "aa": "MSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV", "aa_hexdigest": "357f93e0ad966fff840a283822b7949b", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 100128, "stop": 100892, "observations": { "mutation": [ 100359 ], "stop": [ 100359 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215C93E5", "query_cov": 1.0, "subject_cov": 0.42896935933147634, "identity": 1.0, "score": 299.0, "evalue": 1.35e-99, "gene_start": 1, "gene_end": 154, "reference_start": 206, "reference_end": 359, "reference_sequence": "ANAKKEYSEKYNEALDRNTQAIKDAKLELMEKKREQAESPLVNKIKEIKNRCVLVSKITNVEYTEAVENVFGSLFNSQNITKGSFDTKLKALIFALDQETQNQISEISKDVLTIDEAIVKDVLDVAQRVEITKNLAKRPTQMSGGQQQRVAIARAIVKKPKILLLDEPLSNLDAKLRISTRKWIRSIQQELGITTVFVTHDQEEAMSISDKIVCMSTAKIQQIGSPMELYLKPKNEFVAKFLGMPEMTIFEAQIKDGYIIYNNKKLLKAPEGYQKSSIDVGIRGESLIEEEEGIFEGSIKLVEHLGKEIQAQIFIKELNKIANVFLAKKHNYELGEVVKLNAKSEGLFHLFDVDSKERV" }, "paralog": false, "identity": 0.9960629921259843, "score": 483.0, "evalue": 1.15e-174, "description": "Internal stop codon at 100359. Nonsense mutation around 100359.", "psc": { "uniref90_id": "UniRef90_UPI00215C93E5", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215C93E5", "UniRef:UniRef50_A0A3G8LGF6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGF6" ], "product": "ATP-binding cassette domain-containing protein" } }, "id": "GLJLFKCOHC_202", "locus": "GCA013368035_00162" }, { "type": "cds", "sequence": "contig_1", "start": 100991, "stop": 101266, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATATCAAGTTGGTGCAACGGTTTATGATAAGGGATTTGGACTAAGAAACTATATTAAGTTGTCAAAAGATCCAATATTCCACATCGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATTTCAATTTTAATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGA", "aa": "MLPSLITLTLFTIIPFILVLIFSSTYQVGATVYDKGFGLRNYIKLSKDPIFHIAIRNTLVYAILALPISLAISILISSAISFVIKQSFRRF", "aa_hexdigest": "e30282d6899a0424603a264d62d03497", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10209.208099999989, "isoelectric_point": 10.207359886169431 }, "id": "GLJLFKCOHC_203", "locus": "GCA013368035_00163" }, { "type": "cds", "sequence": "contig_1", "start": 101170, "stop": 101379, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTACCCGCTAGCATGTTAATAAATCCGCCTGATGTTCTAAATAAGAATGCAAAGGCGATTGAAACAGCAATTCCTGATGTTACATATGGTAGAAAGAATACTGTTTGTCAAAATCTACGGAAACTTTGTTTGATTACAAAGCTTATTGCAGATGAAATTAAAATTGAAATCGCCAAGCTAATTGGTAAAGCTAATATAGCATAA", "aa": "MFLPASMLINPPDVLNKNAKAIETAIPDVTYGRKNTVCQNLRKLCLITKLIADEIKIEIAKLIGKANIA", "aa_hexdigest": "51dba5161a87c8001274008457b5bda5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7577.048199999994, "isoelectric_point": 9.342967796325684 }, "id": "GLJLFKCOHC_204", "locus": "GCA013368035_00164" }, { "type": "cds", "sequence": "contig_1", "start": 101456, "stop": 101716, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCCTTTCAAATTCTAATTTTAACAACTGCTATGCTATCAGTTAACCCTGATTTGTATAAATCAGCTTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAGGCAGCTGCAAACTCTGGTATGAGTATCATGCTATATATATTTTGA", "aa": "MAFQILILTTAMLSVNPDLYKSASIDGSSNLKQFFAITLPSIRKTITFLFTVGIIGSIKTFPLALFDNKPAEAAANSGMSIMLYIF", "aa_hexdigest": "0d8d2ad53ba93c1e4304174b0070307b", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9352.989999999993, "isoelectric_point": 9.22402362823486 }, "id": "GLJLFKCOHC_205", "locus": "GCA013368035_00165" }, { "type": "cds", "sequence": "contig_1", "start": 101759, "stop": 101911, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAA", "aa": "MILFLLGLLVSFGLRKLVSLVFFANKKIGEINVIRKIENKTLKRKVVFKI", "aa_hexdigest": "5b9267be914f908d8b08664ff4548470", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5789.191900000001, "isoelectric_point": 11.235179328918456 }, "id": "GLJLFKCOHC_206", "locus": "GCA013368035_00166" }, { "type": "cds", "sequence": "contig_1", "start": 102033, "stop": 102269, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGAAATGTCATTTGTAGAAAAACAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGGGCAATAGTTGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGGTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCGATTACATTAGGATATGCACTATCATTAAGAAATTGA", "aa": "MIEMSFVEKQLSTDSRTTILFPRAIVEGGKSFFPENYRDAFESGFLEALVFTAGITTFSVLIRLGFSITLGYALSLRN", "aa_hexdigest": "41b59fdb68fc65d361c7dd353b33be8b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8719.945599999992, "isoelectric_point": 5.221082115173339 }, "id": "GLJLFKCOHC_207", "locus": "GCA013368035_00167" }, { "type": "cds", "sequence": "contig_1", "start": 102386, "stop": 102571, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTGTTCAGGTTGTTGCTGAAACCATTGGCATTGCTATTTTAAAGAAGTATTTGATTCCATTTGCGCCATCTAGCATTGATGATTCTTTAACACTGTTTGGGATTGCTTCAAATGCATTTTTATACATATATCCAAAGAATATTGAAGCAGCAAACGGCATAACTAATGCAAAGAATTGA", "aa": "MQTVQVVAETIGIAILKKYLIPFAPSSIDDSLTLFGIASNAFLYIYPKNIEAANGITNAKN", "aa_hexdigest": "fe5e88f4344ddbf725467d9010363c68", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6573.5626, "isoelectric_point": 5.97044734954834 }, "id": "GLJLFKCOHC_208", "locus": "GCA013368035_00168" }, { "type": "cds", "sequence": "contig_1", "start": 102793, "stop": 103017, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036432692.1", "SO:0001217", "UniParc:UPI00056AF300", "UniRef:UniRef100_A0A6A8Q0V8", "UniRef:UniRef50_A0A454C920", "UniRef:UniRef90_A0A454C920" ], "nt": "ATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCAAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTGTTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTGTTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAA", "aa": "MQFQDKEMQQKDRIMEKDINVQLVKLSKYQKKSKIKKVFVYIGIGFVLISLIVIMILGVYGIIGGLDGLIKKGL", "aa_hexdigest": "356c950f18a8b3ca111944fbad47724b", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI00056AF300", "ncbi_nrp_id": "WP_036432692.1", "uniref100_id": "UniRef100_A0A6A8Q0V8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00056AF300", "RefSeq:WP_036432692.1", "UniRef:UniRef100_A0A6A8Q0V8" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8Q0V8", "uniref90_id": "UniRef90_A0A454C920" }, "psc": { "uniref90_id": "UniRef90_A0A454C920", "uniref50_id": "UniRef50_A0A454C920" }, "pscc": { "uniref50_id": "UniRef50_A0A454C920", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C920" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8444.260299999994, "isoelectric_point": 9.823321723937987 }, "id": "GLJLFKCOHC_209", "locus": "GCA013368035_00169" }, { "type": "cds", "sequence": "contig_1", "start": 103122, "stop": 103277, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGA", "aa": "MAYGTKNLLIFKNNLIQNKQNLKSFALKLSFYVLKQMPQIKSVILAYDNEY", "aa_hexdigest": "35315d506ca26165cd48c6380d7df148", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5999.050600000001, "isoelectric_point": 9.757241630554198 }, "id": "GLJLFKCOHC_210", "locus": "GCA013368035_00170" }, { "type": "cds", "sequence": "contig_1", "start": 103284, "stop": 104570, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4", "UniRef:UniRef90_D1J7L4" ], "nt": "ATGAGCCAATACGTAACTTATTATTTAAAATCTAGGGGTATAAATGTTTATTTTTTACATAAGAATTATTTAGGAGTTCCTTTTTCATTCTTAGAATTTTCTTTTAAAAAATTAAATACCTTGGATTTGGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAGATTCAAACTATTTTTAAAAATAATCAAGAGATAAATTTTTTTGATTTTAGCAATTTCAACCTCAACGAAAATGAATACCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGCTACTTAATGATTATTTAAACCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAACAAATATCAATATAATTTTGAATTAATTAAGAAGATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAGATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTTTTAACTAGGGGCATGCAGTTTAATTATTTCGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCATATAAAAAATTATTTAATTATGATTTTAAACAAATTTTAAATGAACATTTAGTTGCATTATATATTAAATTTCAAAACACATTCTTTAGCTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAGTTTATATAAACCAACTTTATCCCACTCCTATTTTAATAAAAGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCAACAACAAGACATTATTTTAAACGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGTGCTGAGTATAATTATAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAATTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAATTGAAATTAAAATTCTTAACTTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGACAAATTAAGTATTCAAAAATCAATATCTCGTATTGAAATTGATCAATTAACAGTTTTTGAAAACAAACAAGACAAATATATAAAACATATTGCTAAGATAGCATTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCCTGCAAGCTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAGTATTTTAGCAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAA", "aa": "MSQYVTYYLKSRGINVYFLHKNYLGVPFSFLEFSFKKLNTLDLAIYFSFFDSKSCKIQTIFKNNQEINFFDFSNFNLNENEYQFLNDEPIYLDTKMLLNDYLNQITSNRVTKYHNSILNLIINKYQYNFELIKKIMGFDDLGYKFINKQRFTNDTIFRVSKTFLTRGMQFNYFARFFINQEVKYYLAYKKLFNYDFKQILNEHLVALYIKFQNTFFSSMLINDNFFKINKVYINQLYPTPILIKVCEMFDIEYSFVLQQQDIILNDNENVLYVDNNDISFLSAEYNYRDSFKTFFYINEMLNYFETQQIDIFDIEKELKLKFLTYKEFEIECSIANLVHFKDKLSIQKSISRIEIDQLTVFENKQDKYIKHIAKIALKTNPQCFISISYNEISCKLKFSVFYSQTTKIFNKIKEYFSKFLLLNKKIRN", "aa_hexdigest": "ae83993e84369778d9cbebd383153d4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7L4", "query_cov": 1.0, "subject_cov": 0.8879668049792531, "identity": 0.97, "score": 817.0, "evalue": 1.05e-297, "valid": true, "uniref50_id": "UniRef50_D1J7L4" }, "pscc": { "uniref50_id": "UniRef50_D1J7L4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L4" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 51900.20409999999, "isoelectric_point": 8.84398250579834 }, "id": "GLJLFKCOHC_211", "locus": "GCA013368035_00171" }, { "type": "cds", "sequence": "contig_1", "start": 104586, "stop": 104978, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTGAATAATTTAGAGCTTTATAAAGTTGATAGTAAGGGTAAAAAGATAAATGTTCAAAGATTCCCCAATGGTACAAAAGCAATAGACGCTTTTAATATCTTTAGTGGTTATATTTCTCCTACAAATAAATATCTAAGCCAAGATGCAAAGAACGTAACAAAAAATATTGCCGAAGAAATAGCGTTCACAAACTTTAAACACTTAATCAAAGAAGGATCAATATACAAACCTGCCGATGATGTTGCAACTAGAAGAAGTGGTCAATTAAGAGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAATTTTTGATTACAATGAATTTATTAATAAAATTAAGCTTTTGTTCCAAGAATAA", "aa": "MYTQPLNNLELYKVDSKGKKINVQRFPNGTKAIDAFNIFSGYISPTNKYLSQDAKNVTKNIAEEIAFTNFKHLIKEGSIYKPADDVATRRSGQLRDAIKQAARSILSDANVLKIFDYNEFINKIKLLFQE", "aa_hexdigest": "9644e1e44be13356aef7c3071377277a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14836.778599999981, "isoelectric_point": 9.493501472473145 }, "id": "GLJLFKCOHC_212", "locus": "GCA013368035_00172" }, { "type": "cds", "sequence": "contig_1", "start": 105477, "stop": 105629, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATTACTTCAACCGCTGCTTATCATTACACTTTTGTTAATGAAAATGGAAAAAATAACAATTATACAATCAAGCAGGCAAACAGAGTGTTAAATCCAAACACAACAATTGAAATATTAAACGATACTCCCAAAAATAATGAAAAATGA", "aa": "MVITSTAAYHYTFVNENGKNNNYTIKQANRVLNPNTTIEILNDTPKNNEK", "aa_hexdigest": "1e4b585cdfea5d3e74b66587630b7c0b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5713.2647000000015, "isoelectric_point": 8.085963249206543 }, "id": "GLJLFKCOHC_213", "locus": "GCA013368035_00173" }, { "type": "cds", "sequence": "contig_1", "start": 105684, "stop": 105995, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTAATTAATTTTGCAATTCAAGCAAAAAGATTATACAACGCGGACCCTACATTAAACGTTTTGGGAATAGATGAAATACCTTCGGCTATCAATGTTATGGCAACATCAATCATTGGTAGTGATATCAATAAAAACTATGTAAACCCAGATGATAATTGTCCAACAACTGGTGGCTACAATTATGACAATTTTATTGAAAAACCTGATAGCCCGCAAGCGAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAAGGGTATTCAAGAAGGTGCCGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNADPTLNVLGIDEIPSAINVMATSIIGSDINKNYVNPDDNCPTTGGYNYDNFIEKPDSPQAKLFKKVSSIILKGIQEGAV", "aa_hexdigest": "bf7053e66f07510eb24838d47b32f873", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11316.63539999999, "isoelectric_point": 4.520598411560059 }, "id": "GLJLFKCOHC_214", "locus": "GCA013368035_00174" }, { "type": "cds", "sequence": "contig_1", "start": 106065, "stop": 106352, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTAGCAATTGATGACAAAGATTTTAAAAAATATAACATTGAACCAGTATTTAATGTCGCAAACTCTTTAATTGCAAATAACAAATATAATAAAAAGTGGGCAATACCTCTATCTCGTTCAGGAGAAATGACTACAGTAGCTAAAGCATTAGTTGGAAAATTCGTTAAAGAATTAATGGCTTTAGGAGTTAAATTAGATAGTCAAAATAGTAGCAAATTGCAATCTTATTTAGACTATTACAATAATAACGATCAAGAAGCAAAAGGCGTTGATGGTATTTGA", "aa": "MNLAIDDKDFKKYNIEPVFNVANSLIANNKYNKKWAIPLSRSGEMTTVAKALVGKFVKELMALGVKLDSQNSSKLQSYLDYYNNNDQEAKGVDGI", "aa_hexdigest": "bfae701f8f75282c97b43b67bdfcb081", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10649.020299999995, "isoelectric_point": 8.802851676940918 }, "id": "GLJLFKCOHC_215", "locus": "GCA013368035_00175" }, { "type": "cds", "sequence": "contig_1", "start": 106527, "stop": 106736, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGACTTGGCAAATTATTCACTTTAATGACTATAGGTTTACTTCCTACGACTACATTGATTTTATCCTCATGTTCAACAACAGAAAATTCGAAATACGGATTTGACCAAGAAGATGATGGACAATTAGTATTAGCAATGGGATTTTTAAAAAATGATTGGCAAGGGCTTGCTATTCAAGGGATTGTTGATACATACAACAAATGA", "aa": "MKRLGKLFTLMTIGLLPTTTLILSSCSTTENSKYGFDQEDDGQLVLAMGFLKNDWQGLAIQGIVDTYNK", "aa_hexdigest": "1b271046b7adcdf4911017eae46666bb", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7645.7572, "isoelectric_point": 4.9838361740112305 }, "id": "GLJLFKCOHC_216", "locus": "GCA013368035_00176" }, { "type": "cds", "sequence": "contig_1", "start": 106995, "stop": 107387, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACACAACCATTGAATAATTTAGAACTTTATAAAGTTGATAGCAATGGCAAAAAAACAAACATTCAAAAATTCCCTGATGGTACAAAAGCAATAGATGCTTTCAACGCCTTTGGTGGATATATTTCTCCTACAAATAAATATCTAAGTCAAGATGCAAAGAGTGCAACAAAAAATATTGCTGATGAAATAGCATTCACAAACTTTAAACATTTAATCAATGAAGGATCAATATACAAGCCTGCCGATGATATTGCAACTAGAAAAGGCGACCAATTAAGGGATGCAATAAAGCAAGCAGCTAGATCAATATTGAGTGATGCCAATGTTTTAAAAGTTTTTGACTACAAAGATTTTATTAATAAAATTAAGCTATTGTTCCAAGAATAA", "aa": "MYTQPLNNLELYKVDSNGKKTNIQKFPDGTKAIDAFNAFGGYISPTNKYLSQDAKSATKNIADEIAFTNFKHLINEGSIYKPADDIATRKGDQLRDAIKQAARSILSDANVLKVFDYKDFINKIKLLFQE", "aa_hexdigest": "14657af385a140e6505471275e2336d4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14642.412599999976, "isoelectric_point": 9.001930046081544 }, "id": "GLJLFKCOHC_217", "locus": "GCA013368035_00177" }, { "type": "cds", "sequence": "contig_1", "start": 107514, "stop": 107741, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGCAAGGCAGGCTATATAGTTTCCGGTTCTGAATATTCATACAATAAAGCCGAAAATAAAATTGAATTAAAAATAGGAAATAAAATAACTAAATTACAAGGAATTTATTTAGGCAAATTCTTTAATAATAATGAAAATGAATATTTCTTTATTAATCAAGAAGACGTGGATGTTAAAGTATTAAAGGCCAATAATTTTATTAACCAAGAAGATGCAGATTGA", "aa": "MQGKAGYIVSGSEYSYNKAENKIELKIGNKITKLQGIYLGKFFNNNENEYFFINQEDVDVKVLKANNFINQEDAD", "aa_hexdigest": "f5b968ad2b1bf8254f28b6e34ceab1b8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8616.502799999995, "isoelectric_point": 5.049485206604003 }, "id": "GLJLFKCOHC_218", "locus": "GCA013368035_00178" }, { "type": "cds", "sequence": "contig_1", "start": 107886, "stop": 108044, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTATAAATATTGGTTCTACTGCTGGATATCGTCACACATTCGTTGGTAATAATGAAAAAAATAGTACTTATATAATTAAACAAGCCAACAGGGTATTAGATCCAACAACAACAATTGAAATATTAAATAATACTCCTAAAAATAATGAAAAATGA", "aa": "MAINIGSTAGYRHTFVGNNEKNSTYIIKQANRVLDPTTTIEILNNTPKNNEK", "aa_hexdigest": "9f125fe2d5c33ffda7d748534f0afbda", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5806.436100000001, "isoelectric_point": 9.301836967468262 }, "id": "GLJLFKCOHC_219", "locus": "GCA013368035_00179" }, { "type": "cds", "sequence": "contig_1", "start": 108093, "stop": 108404, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGATAAATTATTTGACAACTATAGCGATTTGATTAATTTTGCAATTCAAGCAAAAAGATTATATAATGTTGACCCTACATTAAATGTTTTAGGAATAGATGAAATACCTTCGGCTATCAATGTTATGGCCACATCAATCATTGGTAGTGATATCAATAAAAACTATATAAACCCAGATGATAATTGCCCTGCAACTGGTGGCTACAATTATGACAATTTTATTGAAAATCCTAATAGTCCACAAGCAAAATTATTTAAAAAAGTTTCTTCTATTATTTTAAAAGGTATCTCAGAAAATGCTGTTTGA", "aa": "MSDKLFDNYSDLINFAIQAKRLYNVDPTLNVLGIDEIPSAINVMATSIIGSDINKNYINPDDNCPATGGYNYDNFIENPNSPQAKLFKKVSSIILKGISENAV", "aa_hexdigest": "c95348207eb1ab4dee7b8d8295cdda18", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11329.633999999987, "isoelectric_point": 4.478082847595215 }, "id": "GLJLFKCOHC_220", "locus": "GCA013368035_00180" }, { "type": "cds", "sequence": "contig_1", "start": 108474, "stop": 108581, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCTGGGAGTTAAATTGGATAGTCAAAATAGTAGCAAATTGCAATCTTATTTAGACTATTACAATAATAATAAACAAGAAGCAGAAGGCGTTGATAGCATTTGA", "aa": "MALGVKLDSQNSSKLQSYLDYYNNNKQEAEGVDSI", "aa_hexdigest": "b5b929f33fbbdbfb23f365f34ac9e090", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3923.2309000000014, "isoelectric_point": 4.436874580383301 }, "id": "GLJLFKCOHC_221", "locus": "GCA013368035_00181" }, { "type": "cds", "sequence": "contig_1", "start": 108657, "stop": 108848, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGACCTAGAAACAAAAAACAGAAAAGATTTTCACAATATTTTATTAAATTACGCATCAGCAGCTGCTTTATATGCAAACCATAAAATGAATTTAGCGGTTGATGACAAAGATTTTAAAAAATATAACATTGAACCTGTATTTAATAATGCAAACTCTTTAATTGCAAATAATAAAGATAACAAAAAATGA", "aa": "MDLETKNRKDFHNILLNYASAAALYANHKMNLAVDDKDFKKYNIEPVFNNANSLIANNKDNKK", "aa_hexdigest": "54875efe247097299132ad564505a3ee", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7271.124200000002, "isoelectric_point": 8.964280509948733 }, "id": "GLJLFKCOHC_222", "locus": "GCA013368035_00182" }, { "type": "cds", "sequence": "contig_1", "start": 108948, "stop": 109157, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTACCAAATTATTTACAGCAGTAGCAGCAGGATTATTTCCCGCAACTGCATTAATTTCAGCCTCGTGCTCAACAACAGAAAATTCAAAATATGGATTTGACCAAGATGATGATGGACAATTAGTATTAGCAACGGGATTTTCAAAAAGTAACTGGCAAGGATTTGCCATTCAAGGAATTGTTAATACATATAATAATTGA", "aa": "MKKITKLFTAVAAGLFPATALISASCSTTENSKYGFDQDDDGQLVLATGFSKSNWQGFAIQGIVNTYNN", "aa_hexdigest": "9ae150dd452904b40b63e5b2c8ed22e4", "start_type": "ATG", "rbs_motif": "AGGAG(G)/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7368.1831, "isoelectric_point": 5.952713584899903 }, "id": "GLJLFKCOHC_223", "locus": "GCA013368035_00183" }, { "type": "cds", "sequence": "contig_1", "start": 109570, "stop": 110064, "strand": "+", "frame": 1, "gene": "rpsD", "product": "30S ribosomal protein S4", "db_xrefs": [ "COG:COG0522", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843", "SO:0001217", "UniRef:UniRef50_Q9PPZ2", "UniRef:UniRef90_D1J7L7" ], "nt": "ATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGAAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCCCCTGGACAACACGGAGCAAAAAGAAGCAAACCATCAGACTATCAACTACACATGTATGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTACTACAAACTGTTGAATCAAGATTAGACAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCACGGACACTTTACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAATTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGACGCTATTGAAGTAATGACAGTTGCTCCATGA", "aa": "MSKFVGSMFKKSRRYGISLLENNKEFTKGKKRTTAPGQHGAKRSKPSDYQLHMYEKQKVRYMYGLNERQFKNLFIKVSKKAGVTGINLLQTVESRLDNLVFRAGFARTRAQARQLVNHGHFTVDGCKANIPSMIVKVGSVIELKEALQNNVAIKDAIEVMTVAP", "aa_hexdigest": "da01c9dae17b2a1d09586afb5cf50a64", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7L7", "query_cov": 1.0, "subject_cov": 0.8241206030150754, "identity": 1.0, "score": 317.0, "evalue": 4.69e-109, "valid": true, "gene": "rpsD", "product": "30S ribosomal protein S4", "uniref50_id": "UniRef50_Q9PPZ2", "cog_id": "COG0522", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PPZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PPZ2" ], "product": "Small ribosomal subunit protein uS4" }, "genes": [ "rpsD" ], "id": "GLJLFKCOHC_224", "locus": "GCA013368035_00184" }, { "type": "cds", "sequence": "contig_1", "start": 110210, "stop": 110521, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63", "UniRef:UniRef90_UPI000AFE6B63" ], "nt": "TTGTTTACTTTTGTAATTATATTAATATATCTAATATCGCTTATTTTGGGTACTCTTTTTTATCAAATTAGTCATGATAGACTCGCAAACTATTTGATTATTGTTTTAAGTTCTGTAGTTTTCCCTGAAATTTTAAACTCTATATATTCACTTTCAAATATAACAATGACGCTTAACCCTTTATACAATAATATTTATCTAATTAATTTAGTTTATATACCAGCATTTATCTTATTTATTGTTTTGATTGCAAAAATAAAAGAAAAACAATTTATATTTTTTAAAAAAGTTAATTATGTTAAAAATAAATAA", "aa": "MFTFVIILIYLISLILGTLFYQISHDRLANYLIIVLSSVVFPEILNSIYSLSNITMTLNPLYNNIYLINLVYIPAFILFIVLIAKIKEKQFIFFKKVNYVKNK", "aa_hexdigest": "ab6b2131a6cb7ffa30c8e9ca9de00814", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 110210, "stop": 110707, "observations": { "mutation": [ 110578, 110659 ], "stop": [ 110578, 110659 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFE6B63", "query_cov": 1.0, "subject_cov": 0.6242424242424243, "identity": 0.893, "score": 163.0, "evalue": 1.49e-49, "gene_start": 1, "gene_end": 103, "reference_start": 63, "reference_end": 165, "reference_sequence": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK" }, "paralog": false, "identity": 0.8787878787878788, "score": 281.0, "evalue": 8.23e-99, "description": "Internal stop codon at 110578, 110659. Nonsense mutation around 110578, 110659.", "psc": { "uniref90_id": "UniRef90_UPI000AFE6B63", "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFE6B63", "UniRef:UniRef50_UPI000AFE6B63" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12086.53669999998, "isoelectric_point": 9.56054859161377 }, "id": "GLJLFKCOHC_225", "locus": "GCA013368035_00185" }, { "type": "cds", "sequence": "contig_1", "start": 110657, "stop": 111007, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q2Z3", "UniRef:UniRef90_D1J7L8" ], "nt": "TTGCTAGCCGGATTTGTAATTTATCTTTTAATATCAGCAATAATATTAAAAATAGATGGTCAAATCGATCAATTTAATCTATTTCCTTTAATAAATCAAATATTTTTAATCGCTCCTTCTTATACTACCCAGCTTCTTTTGGTGTTATTATCAATAGGACTATTTTTAAACTTCTATTTTGTTAAACGATATTTATCAAAACGAAGCATAGAACTTGATCACAAAGAAAAAAGAAGAAACTATTTAATAATAATATTGACGACTGCAATATCATTGATTGTAATATTTTTAATTAACGTAATGATTTTGTTTATTAAACAAAATTTTTATGAAATCGTCTCTGGACATTGA", "aa": "MLAGFVIYLLISAIILKIDGQIDQFNLFPLINQIFLIAPSYTTQLLLVLLSIGLFLNFYFVKRYLSKRSIELDHKEKRRNYLIIILTTAISLIVIFLINVMILFIKQNFYEIVSGH", "aa_hexdigest": "c2269ac5070f0381249e8ec5ca601ea6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 110354, "stop": 111071, "observations": { "insertion": [ 111050 ], "stop": [ 110578, 110659, 111013 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7L8", "query_cov": 1.0, "subject_cov": 0.40559440559440557, "identity": 0.9570000000000001, "score": 204.0, "evalue": 8.26e-64, "gene_start": 1, "gene_end": 116, "reference_start": 22, "reference_end": 137, "reference_sequence": "MEKFVNEKNKKQLLLFLPYWILLAGFVIYLLVSAIMLKIDGQIDQFNLFPLINQIFLIAPSYTTQLLLTLLSIGLFLNFYFVKRYLSKRSIELDHKEKRRNYLIIILTTAISLIVIFLINVMILFIKQNFYKIVSGHWNTKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKIKEKQFIFFKKVNYAKNK" }, "paralog": false, "identity": 0.9327731092436975, "score": 421.0, "evalue": 8.62e-152, "description": "Internal stop codon at 110578, 110659, 111013. Frameshift due to insertion around 111050.", "psc": { "uniref90_id": "UniRef90_D1J7L8", "uniref50_id": "UniRef50_A0A6A8Q2Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7L8", "UniRef:UniRef50_A0A6A8Q2Z3" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q2Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q2Z3" ], "product": "ABC transporter permease" } }, "id": "GLJLFKCOHC_226", "locus": "GCA013368035_00186" }, { "type": "cds", "sequence": "contig_1", "start": 111239, "stop": 111805, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L9", "UniRef:UniRef90_D1J7L9" ], "nt": "ATGATTTGTTCTAGATTACTATTGCCTTTGAATGATGTTGATGAATATAAATTAATTCCTCTTGTTAGAACAATCGAATTTACAATTTATACAAAGGCTTCAAAAATTAAACACGATAACGAAGTACTTCTAACTTCAATATCAAATAATTTAAGTCAATATGACATTGATAATTTTCAAGGTTTATATTGCGATATAAACCAAGCATTTGTTGCTGATAATCAATTATTTGATGAAGAAACAGAGTACCAATTTAAATTCAGCAATTCAAATGATGAAGATAATTACCAAGCAAGTTATATTATTCAAAAACTAATAAAAAAACTATTAAATTTTGTCAATGATGAAGACCTTAATTATTGCTTTATTATTATGACAAAAATCCAAAATAATATTATTAAACCTTTTTATATATATTGCAACCCAGAAGATGCAAAAAAAGAACTTGAACAACTATTTAAAACATTAGATAATACAAAATACGAAGCATTACTATTAGAAGCAACAAATACTTTTAGTTTTGAATTAAAAAAATTTAATGAGGAATATTTAAACAAATCTAGTTGA", "aa": "MICSRLLLPLNDVDEYKLIPLVRTIEFTIYTKASKIKHDNEVLLTSISNNLSQYDIDNFQGLYCDINQAFVADNQLFDEETEYQFKFSNSNDEDNYQASYIIQKLIKKLLNFVNDEDLNYCFIIMTKIQNNIIKPFYIYCNPEDAKKELEQLFKTLDNTKYEALLLEATNTFSFELKKFNEEYLNKSS", "aa_hexdigest": "eea2725ffe320e4396b9a0952b9a4b20", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J7L9", "query_cov": 1.0, "subject_cov": 0.8663594470046083, "identity": 0.9840000000000001, "score": 364.0, "evalue": 8.47e-127, "valid": true, "uniref50_id": "UniRef50_D1J7L9" }, "pscc": { "uniref50_id": "UniRef50_D1J7L9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7L9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22238.924600000028, "isoelectric_point": 4.583007621765137 }, "id": "GLJLFKCOHC_227", "locus": "GCA013368035_00187" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 111819, "stop": 111915, "strand": "-", "gene": "Bacteria_small_SRP", "product": "Bacterial small signal recognition particle RNA", "score": 47.8, "evalue": 4e-05, "db_xrefs": [ "RFAM:RF00169", "SO:0000655" ], "nt": "TTAGAATGCCATGAGAATGATAACCTTGTAACTTGGTCAGGATAGAAATATAGCAGCCATATCGAGAAGTATTATGTCTTGTGGTTTTTTTATTAGA", "id": "GLJLFKCOHC_43", "locus": "GCA013368035_00188" }, { "type": "cds", "sequence": "contig_1", "start": 112108, "stop": 112425, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63", "UniRef:UniRef90_UPI000AFE6B63" ], "nt": "TTGTTTACTTTTGTAATTATATTAATATATCTAATATCACTTATTTTAGGCACATTGTTTTATCAAATTAGCCATGATAGATTTTTAAACTACCTAATTATTATTTTAAGTTCCATTGTTTTTCCCGAAATTTTAAACTCTATATATTCACTTTCAAATATAACAATGACACTTGGTTCTTTATACAATAATATTTACTTAATAAATTTAGTTTATGTGCCTTCATTTATCTTATTTTTTGTTTTAATCGCAAAAATCAAAGAAAAAAAATTCATATTTTTTAAGAAATTTAATACCATTAAAAATAAGAAACTATAA", "aa": "MFTFVIILIYLISLILGTLFYQISHDRFLNYLIIILSSIVFPEILNSIYSLSNITMTLGSLYNNIYLINLVYVPSFILFFVLIAKIKEKKFIFFKKFNTIKNKKL", "aa_hexdigest": "e2f4d0bd1640f92e9b7030a35473669e", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 112114, "stop": 112611, "observations": { "mutation": [ 112482 ], "stop": [ 112482 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000AFE6B63", "query_cov": 0.9809523809523809, "subject_cov": 0.6242424242424243, "identity": 0.816, "score": 147.0, "evalue": 1.95e-43, "gene_start": 1, "gene_end": 103, "reference_start": 63, "reference_end": 165, "reference_sequence": "MILFINQSYYRIKDGRDHSKTPVYLENTKIIEYFYVYIFRLTYWFLLSFLLIKITNNFKKAILFTFVIILIYLISLILGILFYQISHDRLTNYLIIVLSSIVFPEILNTLFSLSNITMTLNSLCNNIYLINLVYIPAFILFIVLITKTKEKQFIFFKKVNYVKNK" }, "paralog": false, "identity": 0.8666666666666667, "score": 283.0, "evalue": 2.18e-99, "description": "Internal stop codon at 112482. Nonsense mutation around 112482.", "psc": { "uniref90_id": "UniRef90_UPI000AFE6B63", "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000AFE6B63", "UniRef:UniRef50_UPI000AFE6B63" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000AFE6B63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000AFE6B63" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12400.958699999976, "isoelectric_point": 9.77767810821533 }, "id": "GLJLFKCOHC_228", "locus": "GCA013368035_00189" }, { "type": "cds", "sequence": "contig_1", "start": 112480, "stop": 112611, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTATTTATTAATCAAAGTTATTATAGAATAAAGGATGGAAGGGATCATTCAAAAATTCCTGTATATTTAGAAAATACAAAAATTATTGAATATTTTTATGTTTATATTTTTAGATTAGCTTATTGA", "aa": "MILFINQSYYRIKDGRDHSKIPVYLENTKIIEYFYVYIFRLAY", "aa_hexdigest": "136a44cb7455806aab52e3ba8eb7c70e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5398.236499999999, "isoelectric_point": 9.048863029479982 }, "id": "GLJLFKCOHC_229", "locus": "GCA013368035_00190" }, { "type": "sorf", "sequence": "contig_1", "start": 113140, "stop": 113166, "strand": "-", "gene": "mgrB", "product": "MgrB", "frame": 2, "db_xrefs": [ "SO:0001217", "UniParc:UPI00051CBDFA", "UniRef:UniRef100_A0A097F1Q3" ], "aa": "MKKLRWFY", "aa_hexdigest": "240d3d40d40b4070f9b94efaad3a16ee", "nt": "ATGAAGAAACTCAGATGGTTCTACTAA", "ups": { "uniparc_id": "UPI00051CBDFA", "uniref100_id": "UniRef100_A0A097F1Q3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00051CBDFA", "UniRef:UniRef100_A0A097F1Q3" ] }, "ips": { "uniref100_id": "UniRef100_A0A097F1Q3", "product": "MgrB" }, "genes": [ "mgrB" ], "id": "GLJLFKCOHC_1227", "locus": "GCA013368035_00191" }, { "type": "cds", "sequence": "contig_1", "start": 113381, "stop": 113638, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAAAGTAGGATATGTAGATCAAACTTTTTTGAGTGATAAATACAATGATACTTCTGGATTATGTGAATATGTTGCAATATCAAATATTTTACTTTATAATGAACTTTTTAATCAAAGTGGTATTTTTACAAATGAAGAATTTAATGATTTTTTTAAATATGATAATTATGACGATTCTATAGAAAATTCATCGCCTATTTTTAGATATTATCAATATGATCATCCTGATTATTCATTAGTTGCTAAATTATGA", "aa": "MQKVGYVDQTFLSDKYNDTSGLCEYVAISNILLYNELFNQSGIFTNEEFNDFFKYDNYDDSIENSSPIFRYYQYDHPDYSLVAKL", "aa_hexdigest": "0d67e6e40e368d7602e99592bf5931ae", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10088.896599999993, "isoelectric_point": 4.08794002532959 }, "id": "GLJLFKCOHC_230", "locus": "GCA013368035_00192" }, { "type": "cds", "sequence": "contig_1", "start": 113654, "stop": 114085, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGAAAATAAAAATTCCTGCATTAACAATAGTAGCAGCAATGCCTTGTTGCTTATTGTCTAGCAATATTAACAACAATGAAAACTATAAAGAATTAAAAGAAAAAGTAACCAATACTATTCAAAGATTTTTTGGAAAAAATCAACAATTTAAATATATAAAAAATTTAAATAATGAATATTTATACATTCAATGTTCTAACTATTATGCTATCGTTGATAAAAACGATTTTTCAATTACTGAAATCAAAAAGGGTTTTCATTCTTATAATGAAAACATAAAATATAATTTTAATTATTTAGAAAAAGAAAATAAAAACTTTAATGAATTAAGAATGGCATATAAGAAAGATTATTTTTCTCCGGTTTTGGAAGACAAACTAGATACATTGCCAAATATAAGCAATATGGTTAAAAATAGTTGGTGGTGA", "aa": "MMKIKIPALTIVAAMPCCLLSSNINNNENYKELKEKVTNTIQRFFGKNQQFKYIKNLNNEYLYIQCSNYYAIVDKNDFSITEIKKGFHSYNENIKYNFNYLEKENKNFNELRMAYKKDYFSPVLEDKLDTLPNISNMVKNSWW", "aa_hexdigest": "dd160841b06cab319db7508b42d042e0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17132.473099999996, "isoelectric_point": 8.98233165740967 }, "id": "GLJLFKCOHC_231", "locus": "GCA013368035_00193" }, { "type": "cds", "sequence": "contig_1", "start": 114379, "stop": 114540, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGACAAAAATAATAAAGTAAGTGGAAACACACATACAAGACAACAAATGAATAATTATTCAAATCAAAATAATTCTAATAATTCAGCATACAGATCAAATTTAAACAATCATTCTAATCAATTGAACCCAAATAATTCAGCATATAGATCAAATTGA", "aa": "MNDKNNKVSGNTHTRQQMNNYSNQNNSNNSAYRSNLNNHSNQLNPNNSAYRSN", "aa_hexdigest": "5607500487b2627702f49f9b64f1ac7d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6085.255500000002, "isoelectric_point": 10.160942649841306 }, "id": "GLJLFKCOHC_232", "locus": "GCA013368035_00194" }, { "type": "cds", "sequence": "contig_1", "start": 114712, "stop": 114888, "strand": "-", "frame": 2, "gene": null, "product": "putative dsRNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC", "UniRef:UniRef90_UPI00215D72BC" ], "nt": "ATGTTAGATATTGAACATTTTTTTAGAGATGTTGATTGCAGAGAAAAAAGACCAAATAAATTTAAATTAGAAAATGCTATCAATACATTAAAAGAATTAGCAGAGCGTGGTGAATGCTCAATGCCAATATATCAAATATCAGAAAAACCTATAATTGATGATAACGGCAAACAATGA", "aa": "MLDIEHFFRDVDCREKRPNKFKLENAINTLKELAERGECSMPIYQISEKPIIDDNGKQ", "aa_hexdigest": "2b959ae2fb5c4702d310e369950de1ab", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 114550, "stop": 114978, "observations": { "mutation": [ 114672, 114711, 114714, 114924 ], "stop": [ 114672, 114711, 114714, 114924 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D72BC", "query_cov": 1.0, "subject_cov": 0.4084507042253521, "identity": 0.983, "score": 119.0, "evalue": 1.75e-33, "gene_start": 1, "gene_end": 58, "reference_start": 31, "reference_end": 88, "reference_sequence": "MKADLFEAIIGAIAIDSGWDIKRLKNIIIKMLDIERFFRDVDCREKRPNKFKLENAINTLKELAERGECSMPIYQISEKPIIDDNGKQWWHCRCNVNFRNLQWMGLWTKYTEGYGTSKKLAKKMAAYNMLCDHYGLPNEYKN" }, "paralog": false, "identity": 0.9436619718309859, "score": 273.0, "evalue": 2.28e-96, "description": "Internal stop codon at 114672, 114711, 114714, 114924. Nonsense mutation around 114672, 114711, 114714, 114924.", "psc": { "uniref90_id": "UniRef90_UPI00215D72BC", "product": "putative dsRNA-binding protein", "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D72BC", "UniRef:UniRef50_UPI00215D72BC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D72BC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D72BC" ], "product": "putative dsRNA-binding protein" } }, "id": "GLJLFKCOHC_233", "locus": "GCA013368035_00195" }, { "type": "cds", "sequence": "contig_1", "start": 114922, "stop": 115233, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease III domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7M0", "UniRef:UniRef90_D1J7M0" ], "nt": "TTGAGCTTTTGCGTAGTTCAACTAATGGCAGAAGAATTTGGCTTTTTAAAATCGGCTTCGCAATATTACAACAAATATGAAGATCTTGAAGAATATTGCCTTATAGCCAATAAAAATGAATCCGATTTTACTGATTTAAAAAATCAAATCGTAAGCAATAAAACACTAGCTAAAAAAATTAATAAATTAGGTTTTGCTAAGTATTTAGTTTTAGGAGATAATGATAAAAGAAATAATGTTGCTAATCAAGATAGTGTGAAAGCTGATTTATTTGAAGCTATAATTGGTGCAATAGCAATTGACAGTGGTTGA", "aa": "MSFCVVQLMAEEFGFLKSASQYYNKYEDLEEYCLIANKNESDFTDLKNQIVSNKTLAKKINKLGFAKYLVLGDNDKRNNVANQDSVKADLFEAIIGAIAIDSG", "aa_hexdigest": "ba377267acb52a6791034de7819143f2", "start_type": "TTG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 114619, "stop": 115209, "observations": { "mutation": [ 114672, 114711, 114714, 114924 ], "stop": [ 114672, 114711, 114714, 114924 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M0", "query_cov": 0.9223300970873787, "subject_cov": 0.4337899543378995, "identity": 1.0, "score": 185.0, "evalue": 1.72e-57, "gene_start": 9, "gene_end": 103, "reference_start": 1, "reference_end": 95, "reference_sequence": "MAEEFGFLKSASQYYNKYEDLEEYCLIANKNESDFTDLKNQIVSNKTLAKKINKLGFAKYLVLGDNDKRNNVANQDSVKADLFEAIIGAIAIDSGWDIKRLKNIIIKMLDIEHFFKDVDCREKRPDKFKLENAINTLKELAERGECSMPIYQISEKPIIDNNGKQWWHCRCNVNFRNLQWMGLWTKYTEGYGTSKKLAKKMAAYIVLCDHYGLPNEYKN" }, "paralog": false, "identity": 0.9642857142857143, "score": 380.0, "evalue": 6.47e-137, "description": "Internal stop codon at 114672, 114711, 114714, 114924. Nonsense mutation around 114672, 114711, 114714, 114924.", "psc": { "uniref90_id": "UniRef90_D1J7M0", "product": "Ribonuclease III domain-containing protein", "uniref50_id": "UniRef50_D1J7M0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M0", "UniRef:UniRef50_D1J7M0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7M0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7M0" ] } }, "id": "GLJLFKCOHC_234", "locus": "GCA013368035_00196" }, { "type": "cds", "sequence": "contig_1", "start": 115618, "stop": 115983, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTTCTTTTATTTAAAAATAAATTTAAGCGTATATATTAATTTTTGCAAAATAATATTGCAATGTGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTACCAAACTCAAAGCCAATGCCCGCAATATTAAATGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGACTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAGTAAAGAAGAATTGTTTTTAATTGATAGATGA", "aa": "MTFFYLKINLSVYINFCKIILQCEFYLILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKPMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEISKEELFLIDR", "aa_hexdigest": "5bc1067ade02536ba69f326dcc43b66a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14327.689899999983, "isoelectric_point": 5.096888923645018 }, "id": "GLJLFKCOHC_235", "locus": "GCA013368035_00197" }, { "type": "cds", "sequence": "contig_1", "start": 116110, "stop": 116568, "strand": "+", "frame": 1, "gene": null, "product": "Putative gamma-glutamylcyclotransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5", "UniRef:UniRef90_A0A2K9YSY5" ], "nt": "ATGGGATTTTCTAGAAATCCTAATGCTAATGAAAATAATTTAAATGCTTTTATTGAAATGGAAAAAGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGACATAAACGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCTTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCATGGATCAATTTTTGCAATTAAAGAAGATGGCAAAATGTATGGAGCCTTAACTTCGTTTAGTCAAAAGTCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCAGAAATAACACTAAAATCATTATATGATAATACAAACATTGATTTTTTAATTAATAAAAAACCCGTTTATTATTTAAGCTCAAGAGAAGACAAAGCAATCAATGAAACCCAAGTTGGTTGA", "aa": "MGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYGALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKAINETQVG", "aa_hexdigest": "343ca302d097564e990a8e3e4d42959b", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 115810, "stop": 116682, "observations": { "mutation": [ 115981, 116566 ], "stop": [ 115981, 116566 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A2K9YSY5", "query_cov": 1.0, "subject_cov": 0.45645645645645644, "identity": 0.987, "score": 300.0, "evalue": 1.88e-100, "gene_start": 1, "gene_end": 152, "reference_start": 144, "reference_end": 295, "reference_sequence": "QYEFYLILAFYNNLNNIIFMYLGEIMSNEEKIYVFSYGTIQDPQFYKELLPNSKQMPAILNGYAKCVDETMYFLLKKDLSSQVKGSVFEISKEELFLIDRWELFPQYQRFQVNVLLTETNEILENVYVYTKLEVGKYYLATDDMGFSRNPNANENNLNAFIEMEKAIKDFPLTDYIFLYDINEQEFEEINKLTHPYAALIIDDKENRNYVAIHGSIFAIKEDGKMYAALTSFSQKSNLNSIFYYQAFNEKLLNSKPEITLKSLYDNTNIDFLINKKPVYYLSSREDKTINETQVGWYENKAFELVEKDFDIDPFIRFNKMLKAFFDTKQKNDK" }, "paralog": false, "identity": 0.9827586206896551, "score": 568.0, "evalue": 9.6e-209, "description": "Internal stop codon at 115981, 116566. Nonsense mutation around 115981, 116566.", "psc": { "uniref90_id": "UniRef90_A0A2K9YSY5", "product": "Putative gamma-glutamylcyclotransferase", "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A2K9YSY5", "UniRef:UniRef50_A0A2K9YSY5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2K9YSY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2K9YSY5" ], "product": "Putative gamma-glutamylcyclotransferase" } }, "id": "GLJLFKCOHC_236", "locus": "GCA013368035_00198" }, { "type": "cds", "sequence": "contig_1", "start": 116789, "stop": 118378, "strand": "+", "frame": 2, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "db_xrefs": [ "COG:COG0322", "COG:L", "GO:0006289", "GO:0009380", "GO:0009381", "KEGG:K03703", "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9", "UniRef:UniRef90_D1J7M2" ], "nt": "ATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCCGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTATAAACCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGATATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCTGACAACAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAGGATGGAATCATAATTGAAAAACAAAGTTCTGAATTTGCAAAGCAGAGATTTGAACAAGCCAAATCAATTATGAAATTTGGCCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAAATGAACCTGTTTGAGCAGGCGAGATTTTATGACCAAATTTTAGATTTATTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAATATCAAAACTATATGTTGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTATGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTTATTGATGAATTTTATGTTAATAATCAATTACCGGCTCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAACGACAAAATAAGCGCGCCATTGAACAAATTAAACAAAAACTTGATTGATATTGCTATTGAGAATGCATCAAATAATATTGAACAAAAAATAAAGAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCAATAAATTATGTTCATATTTGAATGTTTATGCAGAAAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGAGCAGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCTGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTTGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGCTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAACAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAGGTTGACAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATTAATTATTTTGGCAGTTATAAAAAAATTCAACAAGCTTCCTTTGATGAAATATCAAAGGTAGCAAACAAAAAAGTTGCTAAGCTAATTTGTGAAGACAAAGGGGAATCGTTTTATTAA", "aa": "MLQYFDIKMQNSFKTEKMRQEIADFSTIILSSEREAFIEERKLIDFYKPFYNVLFPTRDSFPYIRVKLLSTNKLIIDIKNHYKKEPNSIYYGPIPDNKNFKPLIRYLNHLLLSKDGIIIEKQSSEFAKQRFEQAKSIMKFGPDFKRNLKQKINEAIEMNLFEQARFYDQILDLLNYNKQSQNIFIKTIKDIDVFGFYQYQNYMLVNVMIYKNGSLVNSYDYIFEIILNEHEFVNQFIDEFYVNNQLPAQIIIPLKYYELGGFDYNDKISAPLNKLNKNLIDIAIENASNNIEQKIKNYLSKQTKDNEIFNKLCSYLNVYAERIVLFDNSFLSNTNEVIGAACYFKNGQAFKKFYRFYNLKLNLLRNADVEYMQQTSFNYLKDFGDQVDVVIVDGSIGQINEVKKSMAFLNLNIPLFGLVKNSKHETQKLIDSNNNEIKIDDQDVFNLLSRMQTEVDRFAKYRYNQKQRKSMLSNPLLKIKGIGFKTIEKLINYFGSYKKIQQASFDEISKVANKKVAKLICEDKGESFY", "aa_hexdigest": "26abe136c4fd6bfc256b0b4aecf368cd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M2", "query_cov": 1.0, "subject_cov": 0.921602787456446, "identity": 0.985, "score": 1011.0, "evalue": 0.0, "valid": true, "gene": "uvrC", "product": "excinuclease ABC subunit UvrC", "uniref50_id": "UniRef50_A0A2Z5IPW9", "cog_id": "COG0322", "cog_category": "L", "kegg_orthology_id": "K03703", "go_ids": [ "GO:0006289", "GO:0009380", "GO:0009381" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IPW9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IPW9" ], "product": "Excinuclease cho" }, "genes": [ "uvrC" ], "id": "GLJLFKCOHC_237", "locus": "GCA013368035_00199" }, { "type": "tRNA", "sequence": "contig_1", "start": 118443, "stop": 118518, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(tct)", "amino_acid": "Arg", "anti_codon": "tct", "score": 76.3, "nt": "GGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 118476, 118478 ], "id": "GLJLFKCOHC_4", "locus": "GCA013368035_00200" }, { "type": "tRNA", "sequence": "contig_1", "start": 118588, "stop": 118661, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(tcc)", "amino_acid": "Gly", "anti_codon": "tcc", "score": 71.2, "nt": "GCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 118620, 118622 ], "id": "GLJLFKCOHC_5", "locus": "GCA013368035_00201" }, { "type": "tRNA", "sequence": "contig_1", "start": 118664, "stop": 118737, "strand": "+", "gene": "trnG", "product": "tRNA-Gly(gcc)", "amino_acid": "Gly", "anti_codon": "gcc", "score": 70.8, "nt": "GCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCA", "db_xrefs": [ "SO:0000260" ], "anti_codon_pos": [ 118696, 118698 ], "id": "GLJLFKCOHC_6", "locus": "GCA013368035_00202" }, { "type": "cds", "sequence": "contig_1", "start": 119182, "stop": 119598, "strand": "+", "frame": 1, "gene": null, "product": "FMN-dependent NADH-azoreductase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W7GCQ1" ], "nt": "ATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAGAAATAATATTAACGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCATTAACTGACAAGAACTTCAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTATAATCAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATTGCAACGCAAGGAGCTCCATTAGGTTGA", "aa": "MAKVLIIYGSVIPRENSISTKMLDLFVDEYKRNNINDEIKILDLNNEDMYSLTDKNFKLFYSEENSDKYINLLKSVDKVVMNAPMINFNIPTIIKAFFDRIAVANKTFSYKYASKGSSIGLLNNLKVQIIATQGAPLG", "aa_hexdigest": "192337f9037dd954a8548cfbf08c4d5f", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A2W7GCQ1", "query_cov": 0.8478260869565217, "subject_cov": 0.9212598425196851, "identity": 0.556, "score": 120.0, "evalue": 1.34e-32, "valid": false, "product": "FMN-dependent NADH-azoreductase", "uniref50_id": "UniRef50_A0A2W7GCQ1" }, "pscc": { "uniref50_id": "UniRef50_A0A2W7GCQ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W7GCQ1" ], "product": "FMN-dependent NADH-azoreductase" }, "genes": [], "id": "GLJLFKCOHC_238", "locus": "GCA013368035_00203" }, { "type": "cds", "sequence": "contig_1", "start": 119878, "stop": 120300, "strand": "+", "frame": 1, "gene": "rplK", "product": "50S ribosomal protein L11", "db_xrefs": [ "COG:COG0080", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02867", "SO:0001217", "UniRef:UniRef50_B3PM28", "UniRef:UniRef90_D1J7M4" ], "nt": "ATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCTGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATATAAATTACCAGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGA", "aa": "MAKKVVKKAKLQFAAGQAKPGPALAGVGINMPEFTKAFNDKTRERGNEPVPVLITVYEDKSFEFKLFTSPTSYKLIEAAKIKKGSGVANKEKVGSITIDQLRAIAEYKLPDLNTTKIDSAMKQVAGTAKNMGITVEGYDE", "aa_hexdigest": "c988e0254890aaae286c794b34012d8d", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J7M4", "query_cov": 1.0, "subject_cov": 0.9523809523809523, "identity": 1.0, "score": 271.0, "evalue": 8.2e-92, "valid": true, "gene": "rplK", "product": "50S ribosomal protein L11", "uniref50_id": "UniRef50_B3PM28", "cog_id": "COG0080", "cog_category": "J", "kegg_orthology_id": "K02867", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM28", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM28" ], "product": "Large ribosomal subunit protein uL11" }, "genes": [ "rplK" ], "id": "GLJLFKCOHC_239", "locus": "GCA013368035_00204" }, { "type": "cds", "sequence": "contig_1", "start": 120321, "stop": 121013, "strand": "+", "frame": 3, "gene": "rplA", "product": "50S ribosomal protein L1", "db_xrefs": [ "COG:COG0081", "COG:J", "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02863", "RefSeq:WP_114095899.1", "SO:0001217", "UniParc:UPI000DEDA28F", "UniRef:UniRef100_A0A6A8PZQ7", "UniRef:UniRef50_Q4A5E1", "UniRef:UniRef90_D1J7M5" ], "nt": "ATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTTCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCTGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTATTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCCGATCCTAAAATGATGTTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAAGCTGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGTGGAATTGTTCACGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTCTAGCAGAAAATGCTGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAAGGTACATATGTTTTAAATATTACCGTTTCAACTTCAATGGGTCAATCTGTTAAAGTAAAAATTGATTAA", "aa": "MAKRITKNIKNVKSLVNKKDVFPLADAIELAKKASFAKFDESVDIAIRLNLDTRKSDQQLRGSVVLPHGTGKSVKVLVATDEVSAQKASLEAGADFVYSSAELPEVLNQDKYDFDVIVADPKMMLVLGKFGKKLGPKGLMPNPKTGTVTINPAKAVEELKKGKANYRADKGGIVHASFGKKSMETNVLAENADTIIQTIKRLKPQTVKGTYVLNITVSTSMGQSVKVKID", "aa_hexdigest": "c5c242ad0634006481924b8fe763ecd6", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI000DEDA28F", "ncbi_nrp_id": "WP_114095899.1", "uniref100_id": "UniRef100_A0A6A8PZQ7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DEDA28F", "RefSeq:WP_114095899.1", "UniRef:UniRef100_A0A6A8PZQ7" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PZQ7", "uniref90_id": "UniRef90_D1J7M5" }, "psc": { "uniref90_id": "UniRef90_D1J7M5", "gene": "rplA", "product": "50S ribosomal protein L1", "uniref50_id": "UniRef50_Q4A5E1", "cog_id": "COG0081", "cog_category": "J", "kegg_orthology_id": "K02863", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5E1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5E1" ], "product": "Large ribosomal subunit protein uL1" }, "genes": [ "rplA" ], "id": "GLJLFKCOHC_240", "locus": "GCA013368035_00205" }, { "type": "cds", "sequence": "contig_1", "start": 121124, "stop": 121516, "strand": "-", "frame": 1, "gene": null, "product": "Enolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "nt": "ATGAATAAGAGATTTGGCAACAAAGTTCAACTAGTTGGAGATGACTTAATTGTAACAAATCCAAAATACATTCAAATGGCTATTGACAAGCATGCAATCAATGCATCTTTAATAAAGATAAATCAAATAGGTTCATTAACTGAAACATTTAAGGCAATAACAATGTCACAAAAAGCTGGCTTTGTAGCAATAATTTCTCACCGTTCAGGTGAAACTGAAGATACATTTATTGCAGATTTGGCAGTAGCATTAAATACTGGAGAAATCAAAACCGGCTCTCTTTCAAGAACAGATAGAATTGCTAAATACAATCGCTTGTTAAAAATAGAATCAGAATTAGGCAATGAAGCTAAATTCGAAGGCAAAAACTCATTTTATAATCTAAAAAATTAA", "aa": "MNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIESELGNEAKFEGKNSFYNLKN", "aa_hexdigest": "f9554e5da558142c1aa6a8c873f363d9", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A139MRN9", "query_cov": 0.9923076923076923, "subject_cov": 0.8376623376623377, "identity": 0.636, "score": 169.0, "evalue": 8.08e-52, "valid": false, "product": "Enolase", "uniref50_id": "UniRef50_A0A139PDG5" }, "pscc": { "uniref50_id": "UniRef50_A0A139PDG5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A139PDG5" ], "product": "Enolase" }, "genes": [], "id": "GLJLFKCOHC_241", "locus": "GCA013368035_00206" }, { "type": "cds", "sequence": "contig_1", "start": 121532, "stop": 122299, "strand": "-", "frame": 1, "gene": "eno", "product": "phosphopyruvate hydratase", "db_xrefs": [ "COG:COG0148", "COG:G", "EC:4.2.1.11", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096", "KEGG:K01689", "SO:0001217", "UniRef:UniRef50_P0DXB0", "UniRef:UniRef90_D1J7M6" ], "nt": "GTGCAATTAGCATGCGATAATGTTAATAATATTATTGCTAAAGCTATTATTGGCAAGGAAGTTTCTAAGCAAGAAGAATTAGATAATTTAATGATTAATCTTGATGGAACTGAAACAAAATCAAAATTAGGAGCAAATGCAATATTAGCTGTTTCATTAGCAATTGCAAAGGCTGGCGCCATTGAAGCAAATCTTCCATTATATAAATATTTGGCAAAATTAAACAACCAAGTAGCATACAAAATGCCAGTGCCAATGCTAAACGTTATTAATGGTGGAGAACATGCTTCAAATACTATTGACTTCCAAGAATTTATGATAATGCCTATTGGGGCAAAAACATTTAAAGAATCATTGCAAATGGCAAACTTTGTATTTCATACATTAGCAAAATTATTGAAACAAGCAGGTCATGGAACACAAGTTGGAGATGAAGGTGGATTTGCACCAAACCTACACACTCATGAAGAAGCATTGGACTTTTTAGTAAATGCAATTAAAGTCGCAGGATATAATCCAGCCACTTCAGGAGAAAAAGCAGTAGCAATTTGTTTGGATGCTGCAAGTTCAGAACTTTATCATTCAGATACAAAGTTATATGTATTTAAAAAATTCAAGAAAGCAATTGAAGAAAAACGCCCAGGATTTGAAAAATATCAAAATGTTAAATATTCATTTACTAGCGATGAATTTGTTGAATATTATGGCCATTTAATTGAAAAATACCCAATAATTTCTATTGAAGATTCACATGATGAAAACGATTGA", "aa": "MQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDEND", "aa_hexdigest": "bfac1696c8cd4a683586cc12527d4998", "start_type": "GTG", "rbs_motif": "GGxGG", "genes": [ "eno" ], "truncated": "both", "pseudogene": { "start": 121229, "stop": 122497, "observations": { "mutation": [ 121534, 122317 ], "stop": [ 121534, 122317 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M6", "query_cov": 1.0, "subject_cov": 0.5579868708971554, "identity": 0.996, "score": 495.0, "evalue": 7.28e-174, "gene_start": 1, "gene_end": 255, "reference_start": 67, "reference_end": 321, "reference_sequence": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENNWFGGKGVQLACDNVNNIIAKAIIGKEVSKQEELDNLMINLDGTETKSKLGANAILAVSLAIAKAGAIEANLPLYKYLAKLNNQVAYKMPVPMLNVINGGEHASNTIDFQEFMIMPIGAKTFKESLQMANFVFHTLAKLLKQAGHGTQVGDEGGFAPNLHTHEEALDFLVNAIKVAGYNPATSGEKAVAICLDAASSELYHSDTKLYVFKKFKKAIEEKRPGFEKYQNVKYSFTSDEFVEYYGHLIEKYPIISIEDSHDENDWDGFIKMNKRFGNKVQLVGDDLIVTNPKYIQMAIDKHAINASLIKINQIGSLTETFKAITMSQKAGFVAIISHRSGETEDTFIADLAVALNTGEIKTGSLSRTDRIAKYNRLLKIESELGNEAKFEGKNSFYNLKN" }, "paralog": false, "identity": 0.995260663507109, "score": 820.0, "evalue": 2.81e-304, "description": "Internal stop codon at 121534, 122317. Nonsense mutation around 121534, 122317.", "psc": { "uniref90_id": "UniRef90_D1J7M6", "gene": "eno", "product": "phosphopyruvate hydratase", "ec_ids": [ "4.2.1.11" ], "uniref50_id": "UniRef50_P0DXB0", "cog_id": "COG0148", "cog_category": "G", "kegg_orthology_id": "K01689", "go_ids": [ "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M6", "EC:4.2.1.11", "UniRef:UniRef50_P0DXB0", "COG:COG0148", "COG:G", "KEGG:K01689", "GO:0000015", "GO:0000287", "GO:0004634", "GO:0006096" ] }, "pscc": { "uniref50_id": "UniRef50_P0DXB0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0DXB0" ], "product": "Enolase" } }, "id": "GLJLFKCOHC_242", "locus": "GCA013368035_00207" }, { "type": "cds", "sequence": "contig_1", "start": 122315, "stop": 122497, "strand": "-", "frame": 1, "gene": null, "product": "Phosphopyruvate hydratase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "nt": "ATGTCAAAAATTATTAAGATTAATGCCTATGAAGTTTTAGATAGTAGAGGAAATCCAACAGTTAAAGTTGAATTAAATACTGAACTTGCATATTCTGAAGCATTAGTACCATCAGGAGCTTCAACAGGATCAAAAGAAGCACTTGAATTACGCGATAAAGGAACAAAATACGAAAATAATTGA", "aa": "MSKIIKINAYEVLDSRGNPTVKVELNTELAYSEALVPSGASTGSKEALELRDKGTKYENN", "aa_hexdigest": "6327a23b922b52ad6b82638088f018a6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A920A3H1", "query_cov": 0.9166666666666666, "subject_cov": 0.873015873015873, "identity": 0.527, "score": 55.1, "evalue": 8.27e-09, "valid": false, "product": "Enolase N-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A7V5C5X0" }, "pscc": { "uniref50_id": "UniRef50_A0A7V5C5X0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7V5C5X0" ], "product": "Phosphopyruvate hydratase" }, "genes": [], "id": "GLJLFKCOHC_243", "locus": "GCA013368035_00208" }, { "type": "cds", "sequence": "contig_1", "start": 122701, "stop": 123414, "strand": "+", "frame": 1, "gene": null, "product": "DUF3196 domain-containing protein", "db_xrefs": [ "RefSeq:WP_104940374.1", "SO:0001217", "UniParc:UPI000CD6B110", "UniRef:UniRef100_UPI000CD6B110", "UniRef:UniRef50_A0A3G8LJ90", "UniRef:UniRef90_D1J7M7" ], "nt": "ATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAAATTGAAAAAATTGCAAAAGAAGACCCACTTAAAGCAATTTATATTTTAGATGAAGACAAAAAACAATTCTCTCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAGTTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAACAGTTTAAAAAACAAAAAAATGGGTTATGCATTTATGCTGAGCTACAACGAACTTAAAAAACGAGATGATATAGCAAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGTAATATTGATTTTGAATACGATATTCAAAATAAAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAAAATGCCGAAATTCAAAAGATGCAAGATGAAATTATTGCAACTTTTGACAAAGATATTTCAAAAAACAAGGTTGCAAGGCAAGTGTTTTCAGCGTATTTATTTCAAAATTGGGTTGCAATCTTATTGCATGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACGTCAGATTCACTTTCTGAAGAAGAAAAAGAGTTATATAACATTTTTAAATAA", "aa": "MNNIDNNLFFIEEEIKKIEKIAKEDPLKAIYILDEDKKQFSLKEDLDDLDALRDNIEFQLKKQNLITSTKLDTLSLINSLKNKKMGYAFMLSYNELKKRDDIAKYAIEFQDFFKGNDFDSKGFQTLIYDLLVNSNIDFEYDIQNKKINPKKLGSLLKNAEIQKMQDEIIATFDKDISKNKVARQVFSAYLFQNWVAILLHETKDDYLKISHVIEVILGNQTSDSLSEEEKELYNIFK", "aa_hexdigest": "70ae100e139ae0d83ad13cbffca80129", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000CD6B110", "ncbi_nrp_id": "WP_104940374.1", "uniref100_id": "UniRef100_UPI000CD6B110", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CD6B110", "RefSeq:WP_104940374.1", "UniRef:UniRef100_UPI000CD6B110" ] }, "ips": { "uniref100_id": "UniRef100_UPI000CD6B110", "uniref90_id": "UniRef90_D1J7M7" }, "psc": { "uniref90_id": "UniRef90_D1J7M7", "uniref50_id": "UniRef50_A0A3G8LJ90" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LJ90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LJ90" ], "product": "DUF3196 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_244", "locus": "GCA013368035_00209" }, { "type": "cds", "sequence": "contig_1", "start": 123655, "stop": 124887, "strand": "+", "frame": 1, "gene": "tig", "product": "trigger factor", "db_xrefs": [ "COG:COG0544", "COG:O", "EC:5.2.1.8", "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083", "SO:0001217", "UniRef:UniRef50_A0A501XAI0", "UniRef:UniRef90_D1J7M8" ], "nt": "TTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGATGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTACAAAAAATTGGGTGTTAAATTAGGATCATGTGAATTAACTGAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGACGGCGGAGAAGCTGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATCGGCCTAAAAGCTGGAGAAGAAAAAGATTTAGATTTAGTATTCCCAGAAGATTATCACGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTCGTTAAAACTCCAAATTATCCAACAATCGATGATAATTTTGTAAAGGAAATTAATTTGCCAAATGTTAAAAACGTTGCCGAATTTGAAGAATATGTAAAATTTATTGCTTTAAAGAACAAATTAGCAAATGTTAAAAATGAATTTATTAATGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAAGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTTCTGAAAAAGATTATTACGAATTTTCAAAGAGTACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAACAAATGTTTGTATTAGGTGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAACATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGCGAAATTTTACTAAAAGAAAATGACACAAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGACGAAGCTAAACGTAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAA", "aa": "MDSILDNIYKEQIISQITKDDEIVGHANLRIKDINEEKAEISFVFPLFPEVKLGDYKKLGVKLGSCELTEKDLNDAKSQIASNYVVMLDSKEPIKEKDQVNFNFKGFIDGKPFDGGEAENFDLVIGSKQFIPGFEDKMIGLKAGEEKDLDLVFPEDYHAKNLAGKSVVFHVKINFVKTPNYPTIDDNFVKEINLPNVKNVAEFEEYVKFIALKNKLANVKNEFINEAVKKLSEQSEVSLSESLVEEEADKYYQNFLNNLKQQQISEKDYYEFSKSTKEESLNVFKKQAQINLKQMFVLGAIAKAESLKVTLEDYEKEVEKLAKTYGLAPEHVKQILKFENVENNLINERISEILLKENDTKAFENFDKLLKEVEEYEAKQTQAIVDEAKRKREEAEKIAKESKENIDEQK", "aa_hexdigest": "9cc0543944fcdc42c68958b89192df71", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7M8", "query_cov": 1.0, "subject_cov": 0.8613445378151261, "identity": 0.993, "score": 760.0, "evalue": 1.43e-275, "valid": true, "gene": "tig", "product": "trigger factor", "ec_ids": [ "5.2.1.8" ], "uniref50_id": "UniRef50_A0A501XAI0", "cog_id": "COG0544", "cog_category": "O", "go_ids": [ "GO:0003755", "GO:0005854", "GO:0015031", "GO:0051082", "GO:0051083" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XAI0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XAI0" ], "product": "Trigger factor" }, "genes": [ "tig" ], "id": "GLJLFKCOHC_245", "locus": "GCA013368035_00210" }, { "type": "cds", "sequence": "contig_1", "start": 124979, "stop": 126205, "strand": "+", "frame": 2, "gene": "secA", "product": "preprotein translocase subunit SecA", "db_xrefs": [ "COG:COG0653", "COG:U", "EC:7.4.2.8", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038", "KEGG:K03070", "SO:0001217", "UniRef:UniRef50_Q98RA6", "UniRef:UniRef90_D1J7M9" ], "nt": "ATGAAAATATTTGAATTTAAATCAACTGAAATGAGAATTGCAGAGGCAACCCTTAAAAGGATAAATGCTTTAGAACCAGAAATATCAAAATTAAGCAACGAGAAATTGCAACAAAAAACTATTGAGTTTAAGTCACGTTTAAAAGCAGGAGAAACGCCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGTAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGATTTAGGTTCTGTAGCAGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCTACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTAAGTGTTGGAATAAACAAATCTCAAATGTCAAGCGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTGGGTTTTGACTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGTGGACTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGTGATAATGACGAAAGTAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGTCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTATCTGACAGACAAAGGAATTGAAAAAGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTGCAACAAGCAATTCAAGCAAAAGAACGTGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATTACTTATCAAAATTTTTTCCGTTTATTTAAAAAAATAAGCGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGAGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCACAGCAAATGA", "aa": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEKLQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSK", "aa_hexdigest": "9ed8ef8bc0d8637b3c48016e79168c4d", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034361D20", "query_cov": 0.9534313725490197, "subject_cov": 0.9328537170263789, "identity": 0.581, "score": 444.0, "evalue": 1.55e-151, "valid": false, "product": "DEAD/DEAH box helicase", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_A0A939UGC5", "kegg_orthology_id": "K03070" }, "genes": [ "secA" ], "truncated": "3-prime", "pseudogene": { "start": 124979, "stop": 126508, "observations": { "mutation": [ 126203 ], "stop": [ 126203 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7M9", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 0.998, "score": 778.0, "evalue": 9.22e-277, "gene_start": 1, "gene_end": 408, "reference_start": 1, "reference_end": 408, "reference_sequence": "MKIFEFKSTEMRIAEATLKRINALEPEISKLSNEELQQKTIEFKSRLKAGETPEQIRPETFAVCREATKRILGKRPFDVQMIGGIILDLGSVAEMKTGEGKTITSIAPIYLNAITGQSVIVSTVNEYLAERDAQEMGQVFNFLGLSVGINKSQMSSELKREAYACDIVYSVHSELGFDYLRDNMAMSKEEKVQRGLDFILLDEVDSILIDEAKTPLIISGGDNDESNLYTIADLFVRTLSQDDYFIDEETKSVYLTDKGIEKANKYFNFKNLYDIENSELVHRIQNALRAHKVMKLDVEYIVRNDKIELVDSFTGRIMEGRAYSEGLQQAIQAKERVEIESETKTLATITYQNFFRLFKKISGMTGTAKTEEKEFIDIYNMRVNVVPTNKPIARFDDKDEIYVDMHSKWKAVTEEVKRVYKKKQPILIGTAQVEDSEILHEYLLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDHWKEIFKEYGEDPIPGEAIRKAFLRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLIEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIETWNKCYDQLRQNIIDKYGISSLINSERNIILSVFDAAWQDHINIMDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK" }, "paralog": false, "identity": 0.9960707269155207, "score": 978.0, "evalue": 0.0, "description": "Internal stop codon at 126203. Nonsense mutation around 126203.", "psc": { "uniref90_id": "UniRef90_D1J7M9", "gene": "secA", "product": "preprotein translocase subunit SecA", "ec_ids": [ "7.4.2.8" ], "uniref50_id": "UniRef50_Q98RA6", "cog_id": "COG0653", "cog_category": "U", "kegg_orthology_id": "K03070", "go_ids": [ "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7M9", "EC:7.4.2.8", "UniRef:UniRef50_Q98RA6", "COG:COG0653", "COG:U", "KEGG:K03070", "GO:0005524", "GO:0006605", "GO:0006886", "GO:0017038" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RA6" ], "product": "Protein translocase subunit SecA" } }, "id": "GLJLFKCOHC_246", "locus": "GCA013368035_00211" }, { "type": "cds", "sequence": "contig_1", "start": 126305, "stop": 126625, "strand": "+", "frame": 2, "gene": "secA", "product": "SecA family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "nt": "TTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCAGAAGCAGAAATTATTTCTAAAGCGGGACAAGTAGGAGCAGTTACAATTGCCACAAACATGGCTGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGTGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCAAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGCCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCGTTAGATGACCAATTGATTTTGCGTTTTTCAGTTCAAGATCACTGA", "aa": "MLSEGIPHTVLNAKQDASEAEIISKAGQVGAVTIATNMAGRGTDIKPSKEALELGGLYVLGTEKAESRRIDNQLKGRSGRQGDVGYTKFFLSLDDQLILRFSVQDH", "aa_hexdigest": "4cd803eb0ed4b784c964843ec9d98f33", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A920T749", "query_cov": 0.8113207547169812, "subject_cov": 0.9247311827956989, "identity": 0.698, "score": 119.0, "evalue": 2.96e-33, "valid": false, "product": "SecA family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920T749" }, "pscc": { "uniref50_id": "UniRef50_A0A920T749", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920T749" ], "product": "SecA family profile domain-containing protein" }, "genes": [ "secA" ], "id": "GLJLFKCOHC_247", "locus": "GCA013368035_00212" }, { "type": "cds", "sequence": "contig_1", "start": 126689, "stop": 127030, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTCGATGACGTAATTAGACAACAACGTGATTTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTCTTTATAAAAAACAATAGTTTGGATTTAACAGAATTTGTAAAGCATCTAAATAACAATTTTATGATCTTAACAAATAAGGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGTGAAGAATTAATTCAATACTTAATTGAAACTTGA", "aa": "MRAQKKIEGFNFDNRKSVLNFDDVIRQQRDLIYEQRDLILNRDDLGTIIRKMISVAAEKTVNNPFFIKNNSLDLTEFVKHLNNNFMILTNKEFSVEELVEYDREELIQYLIET", "aa_hexdigest": "1f72255b6d70bd3f48b2009624615bb7", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13531.256399999984, "isoelectric_point": 5.040334129333496 }, "id": "GLJLFKCOHC_248", "locus": "GCA013368035_00213" }, { "type": "cds", "sequence": "contig_1", "start": 127157, "stop": 127573, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCATATCAAGTTTACACTCAATTGGGTTCAAAGAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGTACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAATGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAACCAACAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAGCGTGATCATTTGATTAAAGAAGGTTTAGGCGAACAAGAAGTGGATGATAAACTAAAGGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAA", "aa": "MDRLRRSTNLVQYSQKNPYQVYTQLGSKKFKELTDRIALECTINLMNNYDAIPSTNTDVDLSWLTNAFEEKTDMAQTTSKNQQALEFINYLLKSERDHLIKEGLGEQEVDDKLKEVERSILKEFQFQLASLQTNEEKK", "aa_hexdigest": "fdd96d929476e5d5db4aad5b81cc4d01", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16172.984399999992, "isoelectric_point": 5.129798698425293 }, "id": "GLJLFKCOHC_249", "locus": "GCA013368035_00214" }, { "type": "cds", "sequence": "contig_1", "start": 127581, "stop": 127829, "strand": "-", "frame": 3, "gene": "yjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "db_xrefs": [ "COG:COG1051", "COG:F", "EC:3.6.1.17", "KEGG:K01518", "SO:0001217", "UniRef:UniRef50_D1J7N0", "UniRef:UniRef90_D1J7N0" ], "nt": "TTGCTCAAGGGTTTTAGAGAAGTCAATAAATATATTATTGGAAAATTAATATCTAAAGAAGTTGTTTATTTTATTGCTAAGCCAACATCTTTTGATTTAATTAAGCAAGATAGTGAAATTAAGGTAGTTGAATGGCAAGATACAACAAATGCATTGTTATATCGCATAACATACGAAAGTGATTGTGAAATATTGAAAAAGGCTGTTGAATTTTATTGCGAATATATAACAAAAAATAACGAGTTTTAA", "aa": "MLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF", "aa_hexdigest": "e842433637f69956c87135a143581f8c", "start_type": "TTG", "rbs_motif": null, "genes": [ "yjhB" ], "truncated": "3-prime", "pseudogene": { "start": 127581, "stop": 128012, "observations": { "mutation": [ 127922 ], "stop": [ 127922 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N0", "query_cov": 1.0, "subject_cov": 0.5734265734265734, "identity": 0.988, "score": 167.0, "evalue": 4.67e-52, "gene_start": 1, "gene_end": 82, "reference_start": 62, "reference_end": 143, "reference_sequence": "MKKEKSCGAIIFKEENDNLFVLLVEQTAGHWGFPKGHVEKNETEEETAIREVKEETNIDIKLLKGFREVNKYIIGKLISKEVVYFIAKPTSFDLIKQDSEIKVVEWQDTTNALLYRITYESDCEILKKAVEFYCEYITKNNEF" }, "paralog": false, "identity": 0.986013986013986, "score": 285.0, "evalue": 1.1e-100, "description": "Internal stop codon at 127922. Nonsense mutation around 127922.", "psc": { "uniref90_id": "UniRef90_D1J7N0", "gene": "YjhB", "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)", "ec_ids": [ "3.6.1.17" ], "uniref50_id": "UniRef50_D1J7N0", "cog_id": "COG1051", "cog_category": "F", "kegg_orthology_id": "K01518", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N0", "EC:3.6.1.17", "UniRef:UniRef50_D1J7N0", "COG:COG1051", "COG:F", "KEGG:K01518" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N0" ], "product": "Diadenosine 5'5'''-P1, P4-tetraphosphatepyrophosphohydrolase (MutT/nudix family protein)" } }, "id": "GLJLFKCOHC_250", "locus": "GCA013368035_00215" }, { "type": "cds", "sequence": "contig_1", "start": 127920, "stop": 128012, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAGAGAAGTCGTGTGGCGCTATTGTTTTCAAAGAAGAAAATGATAATCTTTTTGTTTTATTAGTTGAACAAACAGCAGGCCATTGA", "aa": "MKKEKSCGAIVFKEENDNLFVLLVEQTAGH", "aa_hexdigest": "202cb7635261bba9771de4438a00fe12", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3378.8710999999994, "isoelectric_point": 5.573540687561034 }, "id": "GLJLFKCOHC_251", "locus": "GCA013368035_00216" }, { "type": "cds", "sequence": "contig_1", "start": 128210, "stop": 128602, "strand": "-", "frame": 1, "gene": null, "product": "DUF4199 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5", "UniRef:UniRef90_A0A454C9U5" ], "nt": "ATGGATATTAACAATATTATAAAAAATAATATAAAAAGATTATTAATAACAACCGCATTTGGCTTTTTAGCGATTTTGATTTCTTTTTTGATTACCGGGATATTTTCAATACAATCACTTTCAAGTAATAGCCGTCTTGCAATATCTTGCTTTGGACAAATAATTTGCGGTTTTTATGTTGTTTATTATATTTTGAATTTCTTTTTAGTATATAGATTTAAATCAGAAATTGCAAACAAAAAGATATTAAAAACTCTATTAATAATCAGCATTTTTATTTTGATATGTGGAATTTGTTTTATTGTTAATGGTTTAATTTTACCTTCAATTAGAAAAACATACATTCAAGCAGTAATGACTAAAAATAATAATTTATTACAAAGTATACTTTGA", "aa": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSSNSRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSIL", "aa_hexdigest": "face2ad33a967bcdbec6b02ae3056282", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 128099, "stop": 128602, "observations": { "mutation": [ 128182, 128212 ], "stop": [ 128182, 128212 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U5", "query_cov": 1.0, "subject_cov": 0.7784431137724551, "identity": 1.0, "score": 238.0, "evalue": 9.72e-79, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MDINNIIKNNIKRLLITTAFGFLAILISFLITGIFSIQSLSSNSRLAISCFGQIICGFYVVYYILNFFLVYRFKSEIANKKILKTLLIISIFILICGICFIVNGLILPSIRKTYIQAVMTKNNNLLQSILWQGKVIKVEMWISSTIFLVAIASSLILCSFIFKKMNN" }, "paralog": false, "identity": 0.9880239520958084, "score": 312.0, "evalue": 2.32e-110, "description": "Internal stop codon at 128182, 128212. Nonsense mutation around 128182, 128212.", "psc": { "uniref90_id": "UniRef90_A0A454C9U5", "product": "DUF4199 domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U5", "UniRef:UniRef50_A0A454C9U5" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U5" ], "product": "DUF4199 domain-containing protein" } }, "id": "GLJLFKCOHC_252", "locus": "GCA013368035_00217" }, { "type": "cds", "sequence": "contig_1", "start": 128686, "stop": 129570, "strand": "+", "frame": 1, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "db_xrefs": [ "COG:COG2812", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360", "KEGG:K02343", "SO:0001217", "UniRef:UniRef50_D1J7N2", "UniRef:UniRef90_D1J7N2" ], "nt": "GTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGTCCCCATGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTGCATAATCAAAGCGATATATTAAAACCGTGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCGGCCTTTAACGCTTTATTAAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCTCAAAAAATACCAGAAACTATTTTGAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATAGCTAAATTAGGAAATGGTAGCCTTAGAGATTCATTGAGTATTGCAGACCATGTTGCGATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTGTATGTCAATGCATGATATCAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGA", "aa": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKE", "aa_hexdigest": "599f28bc4d3f563ffeffba8cd9517212", "start_type": "GTG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI00244E6DAE", "query_cov": 0.8163265306122449, "subject_cov": 0.967741935483871, "identity": 0.629, "score": 304.0, "evalue": 8.38e-101, "valid": false, "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A7V0IXX6", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "genes": [ "dnaX" ], "truncated": "3-prime", "pseudogene": { "start": 128686, "stop": 129873, "observations": { "mutation": [ 129568 ], "stop": [ 129568 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N2", "query_cov": 1.0, "subject_cov": 0.4407796101949025, "identity": 1.0, "score": 579.0, "evalue": 4.82e-203, "gene_start": 1, "gene_end": 294, "reference_start": 1, "reference_end": 294, "reference_sequence": "MALTNKYLALYRKYRPKIFDDIKGQDHIVTTLKNIILNNKLSHAYLFCGPHGNGKTSTAKVFANTINCLHNQSDILKPCEECIKNVDRNLDIIEIDAASNTGIDDIRELKEKIKHLPTQSKYKIYIIDEVHMLSKAAFNALLKTIEDPPSHIIFILATTDPQKIPETILSRVQRFNFKLMTIETLVSQLKDIFNKENIEYEDDAINVIAKLGNGSLRDSLSIADHVAIYCANKVIKKEVVEQLFGIVNIDNKIELLNCMSMHDINKTLNLARELFSQGIDINILINDLINILKEWIIFNKTRDQSLVSTDFKILLQIKIDDVKAHYYIETLIDQLHSIAISDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQVINKNNIYKSLDLSSIANGFNANNSTIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQISIDSNVDNQETADLQISENWKDLVNEDLISKENSENKHVPLETIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKGEILEAKKYWKANINEIRAKKVKPLEDLNLKYNKTVQKDVAWAQEIFGDKFKLNK" }, "paralog": false, "identity": 0.9898734177215189, "score": 774.0, "evalue": 2.69e-282, "description": "Internal stop codon at 129568. Nonsense mutation around 129568.", "psc": { "uniref90_id": "UniRef90_D1J7N2", "gene": "dnaX", "product": "DNA polymerase III subunit gamma/tau", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_D1J7N2", "cog_id": "COG2812", "cog_category": "L", "kegg_orthology_id": "K02343", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N2", "EC:2.7.7.7", "UniRef:UniRef50_D1J7N2", "COG:COG2812", "COG:L", "KEGG:K02343", "GO:0003677", "GO:0003887", "GO:0005524", "GO:0006260", "GO:0009360" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N2" ], "product": "DNA polymerase III subunit gamma/tau" } }, "id": "GLJLFKCOHC_253", "locus": "GCA013368035_00218" }, { "type": "cds", "sequence": "contig_1", "start": 129628, "stop": 130149, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAATTAAAATTGATGATGTTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCCGTAAGTGATAGTCCAGAAGATTATTTCGAAATTCTATTGCTAAAGTTATGTAATTACTTAAGTCCAAAAGAAGAATACAAAAATTCTGCAGTAATGCCAAAAATTAGCAATGAAGAATTTAATAATTTTAATAGTCAAGAAAAACAGGCCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCGCAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAGACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGCAATAATCAAATTATCATAGATTCAAATGTTGATAACCAAGAAACAGCCGATTTACAAATATCAGAAAATTGA", "aa": "MQIKIDDVKAHYYIETLIDQLHSIAVSDSPEDYFEILLLKLCNYLSPKEEYKNSAVMPKISNEEFNNFNSQEKQAINKNNIYKSLDLSSIANGFNANNSAIQNSSDNNSTEDLANNSKKIDDLLEKTAEIYSLDQTSEFEVADISNQIISNNQIIIDSNVDNQETADLQISEN", "aa_hexdigest": "941cc01f0db0c05268c171847cc7d248", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19564.163199999988, "isoelectric_point": 4.198037147521972 }, "id": "GLJLFKCOHC_254", "locus": "GCA013368035_00219" }, { "type": "cds", "sequence": "contig_1", "start": 130210, "stop": 130647, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAACAATATGTTAAATGATGGCCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAACATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACATGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGCTTATGCCATTACAAAAGGCGAGATATTAGAAGCAAAGAAATATTGGAAAGAAAATATAAACGAAATAAGAGCCAAAAAAGTTAAACCTTTGGAAGATTTAAATTTAAAATACAATAAAACAGTTCAAAAAGATGTTGCTTGA", "aa": "METIIDCLLIKQYLKNNMLNDGRSNFTDIDAIKYGMLSQKITDKNEINILSDFKIIFSTNDFILLTCDIDEKVYDLNINSHNENIMAALNKVFDRYLYAYAITKGEILEAKKYWKENINEIRAKKVKPLEDLNLKYNKTVQKDVA", "aa_hexdigest": "2949c40311fade33050a2fc703aa4d12", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16967.400699999995, "isoelectric_point": 5.8799028396606445 }, "id": "GLJLFKCOHC_255", "locus": "GCA013368035_00220" }, { "type": "cds", "sequence": "contig_1", "start": 130713, "stop": 131012, "strand": "+", "frame": 3, "gene": "ybaB", "product": "YbaB/EbfC family nucleoid-associated protein", "db_xrefs": [ "COG:COG0718", "COG:K", "GO:0003677", "KEGG:K09747", "RefSeq:WP_012855373.1", "SO:0001217", "UniParc:UPI0001BC20AC", "UniRef:UniRef100_D1J7N3", "UniRef:UniRef50_Q98RF9", "UniRef:UniRef90_D1J7N3" ], "nt": "ATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGATGAACAAGAAATTCAAAAGAAGGAATTTACTATTGAAAAGCAAGGCATAACCGTTGTTATGAATGGTTTAAGAAAATTAGTGTCTATAAAATTTAATCCAGCATTAATCGATCCCGATGACCCTGAATTATTAGAAGATTTATTGATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAA", "aa": "MSMNMNEMLKKARRIQTEMEKDEQEIQKKEFTIEKQGITVVMNGLRKLVSIKFNPALIDPDDPELLEDLLMLAVNEAIETVDKAFDEINAKYSDSGLSF", "aa_hexdigest": "77242d963041dcb031811ba880313b74", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20AC", "ncbi_nrp_id": "WP_012855373.1", "uniref100_id": "UniRef100_D1J7N3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20AC", "RefSeq:WP_012855373.1", "UniRef:UniRef100_D1J7N3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N3", "uniref90_id": "UniRef90_D1J7N3" }, "psc": { "uniref90_id": "UniRef90_D1J7N3", "gene": "YbaB", "product": "YbaB/EbfC family nucleoid-associated protein", "uniref50_id": "UniRef50_Q98RF9", "cog_id": "COG0718", "cog_category": "K", "kegg_orthology_id": "K09747", "go_ids": [ "GO:0003677" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RF9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RF9" ], "product": "Nucleoid-associated protein MYPU_0500" }, "genes": [ "ybaB" ], "id": "GLJLFKCOHC_256", "locus": "GCA013368035_00221" }, { "type": "cds", "sequence": "contig_1", "start": 131012, "stop": 131602, "strand": "+", "frame": 2, "gene": "recR", "product": "Recombination protein RecR", "db_xrefs": [ "COG:COG0353", "COG:L", "RefSeq:WP_044329430.1", "SO:0001217", "UniParc:UPI0005C83B92", "UniRef:UniRef100_A0A6A8PYU1", "UniRef:UniRef50_D1J7N4", "UniRef:UniRef90_D1J7N4" ], "nt": "ATGAGCAATATTGAAATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAATTGAAGATATAGACCAAGTTTGCAATCAAATAAAAGCATTGAGAAATTCAATAAAAAAGTGCAACATTTGTAATAGATATAACGAAAATACAATTTGCAATATTTGCTTGGATACTAATCGAACAAAACAACTTATGTTAGTTGGTAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAATTCAAAGAATGAAGATTCTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGCCAATTTAATATGCATTATATTAAAAAGTTCTTGAAAAACAAAATAAATTTTACAAATATTTTTCAATTATCAACAGGTATTCCTTTAGGTTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATTAAAATATAG", "aa": "MSNIEIQKLEELLKTVFDFTKKQSQKFIFNIFKSSIEDIDQVCNQIKALRNSIKKCNICNRYNENTICNICLDTNRTKQLMLVGNSLDIDKFENIGLYKGKYFVFDQNINSKNEDSIVSSLLEKLIDILKDKNELILSLSPTIEGQFNMHYIKKFLKNKINFTNIFQLSTGIPLGSSVEYIDQDTLKESLLNKIKI", "aa_hexdigest": "5515fc29b6a7fb195d5f0b46016062ed", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005C83B92", "ncbi_nrp_id": "WP_044329430.1", "uniref100_id": "UniRef100_A0A6A8PYU1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C83B92", "RefSeq:WP_044329430.1", "UniRef:UniRef100_A0A6A8PYU1" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PYU1", "uniref90_id": "UniRef90_D1J7N4" }, "psc": { "uniref90_id": "UniRef90_D1J7N4", "gene": "RecR", "product": "Recombination protein RecR", "uniref50_id": "UniRef50_D1J7N4", "cog_id": "COG0353", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N4" ], "product": "Recombination protein RecR" }, "genes": [ "recR" ], "id": "GLJLFKCOHC_257", "locus": "GCA013368035_00222" }, { "type": "cds", "sequence": "contig_1", "start": 131614, "stop": 131919, "strand": "+", "frame": 1, "gene": "tmk", "product": "dTMP kinase", "db_xrefs": [ "COG:COG0125", "COG:F", "EC:2.7.4.9", "GO:0004798", "GO:0005524", "GO:0006233", "KEGG:K00943", "SO:0001217", "UniRef:UniRef50_A0A448ZVP3", "UniRef:UniRef90_D1J7N5" ], "nt": "ATGTCAAAAGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAGATTAAAAGAATATTTAGAGTCTGAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCTTTGTTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGCGTTTGGCCAGGATTGGAAAAGAATAAAACGGTTATTTCAGATAGGTATTGA", "aa": "MSKGKFVVFEGMDGSGKTTIIKRLKEYLESENRLDDFVFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLEKNKTVISDRY", "aa_hexdigest": "46988c0b1c08e8f9589ae3bfd7161cd1", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "tmk" ], "truncated": "3-prime", "pseudogene": { "start": 131614, "stop": 132222, "observations": { "mutation": [ 131917, 132142 ], "stop": [ 131917, 132142 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N5", "query_cov": 1.0, "subject_cov": 0.47417840375586856, "identity": 0.97, "score": 199.0, "evalue": 2.21e-63, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MSKGKFVVFEGMDGSGKTTIIKKLKEYLESKNRLDDFIFTREPGSSHSKEAEEIRQLILDNDNSFSVMVDALLFAASRRLNLEKCVWPGLEKNKTVISDRYWTSSFVYQGVLGGLGLKKVKDINNIATEETTPDLTIFFDMEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSYWYVINQGNEKYEIIKSDCNIDELFKRVLNVLKENKII" }, "paralog": false, "identity": 0.9752475247524752, "score": 391.0, "evalue": 1.87e-141, "description": "Internal stop codon at 131917, 132142. Nonsense mutation around 131917, 132142.", "psc": { "uniref90_id": "UniRef90_D1J7N5", "gene": "tmk", "product": "dTMP kinase", "ec_ids": [ "2.7.4.9" ], "uniref50_id": "UniRef50_A0A448ZVP3", "cog_id": "COG0125", "cog_category": "F", "kegg_orthology_id": "K00943", "go_ids": [ "GO:0004798", "GO:0005524", "GO:0006233" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N5", "EC:2.7.4.9", "UniRef:UniRef50_A0A448ZVP3", "COG:COG0125", "COG:F", "KEGG:K00943", "GO:0004798", "GO:0005524", "GO:0006233" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVP3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVP3" ], "product": "Thymidylate kinase" } }, "id": "GLJLFKCOHC_258", "locus": "GCA013368035_00223" }, { "type": "cds", "sequence": "contig_1", "start": 132034, "stop": 132144, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGA", "aa": "MEPELSVERITQIRKTKTDRLESTNVEYYKKLRDSY", "aa_hexdigest": "a43bed897f7f5d7fb1812dc0843d9f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4420.9522000000015, "isoelectric_point": 8.064688682556149 }, "id": "GLJLFKCOHC_259", "locus": "GCA013368035_00224" }, { "type": "cds", "sequence": "contig_1", "start": 132293, "stop": 132448, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAAAAATAAGCTTCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTATTAGATTAGTTAATGATGAAAAACAAATAAGCTGA", "aa": "MINKNLSKVLENSIKKNKLQQVYLLFSRANQDIDLDILEFIRLVNDEKQIS", "aa_hexdigest": "67430c399f4dbe533dc0a8aad951c81f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6033.947799999998, "isoelectric_point": 8.064495277404784 }, "id": "GLJLFKCOHC_260", "locus": "GCA013368035_00225" }, { "type": "cds", "sequence": "contig_1", "start": 132458, "stop": 133126, "strand": "+", "frame": 2, "gene": "dnaX", "product": "DNA polymerase III subunit", "db_xrefs": [ "COG:COG2812", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7N6", "UniRef:UniRef90_D1J7N6" ], "nt": "TTGCAAAAAGAATATTGTTTTATTATTGATGTAAGCATAGATGAAATATCGAAAGAAAATTTACAAGAATTTTTAAATCGTGTTTCCAAAAGCTCTCTATTTGCTAATAAATATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTGAAATTTTTAGAAAATCCTCCATTTAATACAATAATATTATTGCATACAATAAAGCCTCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAATTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATGTTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTACGAATTAAAGAATAGCATAAAAAAAGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAGGCTAATTTTAAAATTAAAGGAAGAATATGAATAA", "aa": "MQKEYCFIIDVSIDEISKENLQEFLNRVSKSSLFANKYKFLIIKCVENITLKAANSILKFLENPPFNTIILLHTIKPQNVLKTIKSRCFIIDIRDDFNAVASVENFFITYKIDVSKQKQFRELADLIYNSPANLFNLILFYVQQLNIENYKNIIHFSIFIFKQIYELKNSIKKDKNISKFDSISILNIINNLYNFVELIDNNNCIFNIQKARLILKLKEEYE", "aa_hexdigest": "5105b3800300b93ffb9e18e4460ba9c5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7N6", "query_cov": 1.0, "subject_cov": 0.8014440433212996, "identity": 0.968, "score": 414.0, "evalue": 6.26e-145, "valid": true, "gene": "DnaX", "product": "DNA polymerase III subunit", "uniref50_id": "UniRef50_D1J7N6", "cog_id": "COG2812", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_D1J7N6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N6" ], "product": "DNA polymerase III subunit" }, "genes": [ "dnaX" ], "id": "GLJLFKCOHC_261", "locus": "GCA013368035_00226" }, { "type": "cds", "sequence": "contig_1", "start": 133119, "stop": 133832, "strand": "+", "frame": 3, "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "db_xrefs": [ "COG:COG0313", "COG:J", "EC:2.1.1.198", "GO:0000451", "GO:0008649", "GO:0008757", "KEGG:K07056", "RefSeq:WP_036432737.1", "SO:0001217", "UniParc:UPI000512E542", "UniRef:UniRef100_UPI000512E542", "UniRef:UniRef50_D1J7N7", "UniRef:UniRef90_D1J7N7" ], "nt": "ATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTACGAAATTAATAACAAACGTTTGATTGCATACCATAAATTTAATGAAAAAGAAATGACACCTAAAATTATTGAATTATTGCAACAAGGGATGATTATTTCATTGATTTCTGATGCCGGTATGCCTTGCATTTCTGATCCTGGTTTTGAAGTTATTAAAGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGCGGACCAACTGCTCTAATACATGCAATAATAAAGGCTAATTTAGACAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCGGGTGCAAGACAAAATCAATTAAAATCCTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAACACAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAA", "aa": "MNNKIYIVGTPIGNLEDITYRAIKVLQSSDIILCEDIRVSQKLLQHYEINNKRLIAYHKFNEKEMTPKIIELLQQGMIISLISDAGMPCISDPGFEVIKAAKAANIGIDVIGGPTALIHAIIKANLDNCFTFLGFLKDKSGARQNQLKSLTFGTYVAYVSPHKLLSTIKDFEIIFQEQVKLFLIKEMTKMFETSFEGTPQEVLTQLSEEDQRGEYTLVFSFKEPKKLKINKYKKEIK", "aa_hexdigest": "d3808ec098ebdbf8e5c9dd3975aecb69", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512E542", "ncbi_nrp_id": "WP_036432737.1", "uniref100_id": "UniRef100_UPI000512E542", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512E542", "RefSeq:WP_036432737.1", "UniRef:UniRef100_UPI000512E542" ] }, "ips": { "uniref100_id": "UniRef100_UPI000512E542", "uniref90_id": "UniRef90_D1J7N7" }, "psc": { "uniref90_id": "UniRef90_D1J7N7", "gene": "rsmI", "product": "16S rRNA (cytidine(1402)-2'-O)-methyltransferase", "ec_ids": [ "2.1.1.198" ], "uniref50_id": "UniRef50_D1J7N7", "cog_id": "COG0313", "cog_category": "J", "kegg_orthology_id": "K07056", "go_ids": [ "GO:0000451", "GO:0008649", "GO:0008757" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N7" ], "product": "Ribosomal RNA small subunit methyltransferase I" }, "genes": [ "rsmI" ], "id": "GLJLFKCOHC_262", "locus": "GCA013368035_00227" }, { "type": "cds", "sequence": "contig_1", "start": 133875, "stop": 134138, "strand": "-", "frame": 3, "gene": "rpsT", "product": "30S ribosomal protein S20", "db_xrefs": [ "COG:COG0268", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02968", "RefSeq:WP_012855378.1", "SO:0001217", "UniParc:UPI0001BC20B1", "UniRef:UniRef100_D1J7N8", "UniRef:UniRef50_Q98PR3", "UniRef:UniRef90_D1J7N8" ], "nt": "ATGGCAAACATTAAATCAAAACAAAAAGCAATATTATCTAACGAAAAAGCAAACATCCGTAATTCAGCCGTTAAATCATCAGTTAAAACAGCTATTAAGAAAGCAAAAATTGCTGCTGTTAATAAAGATCCTAAAGCTGCTGAATTTCAAGCTAATGCTCACCACGAAATTGACAAAGCTGTTTCAAAGGGCGTTTTACATCAAAACAATGGTTCTAGAAAAGCTAGCCGTTTAGATGCATTTATTGCTAAAAATAACAACTAA", "aa": "MANIKSKQKAILSNEKANIRNSAVKSSVKTAIKKAKIAAVNKDPKAAEFQANAHHEIDKAVSKGVLHQNNGSRKASRLDAFIAKNNN", "aa_hexdigest": "19721fc9eea4c2bbc137fa79a30a1504", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC20B1", "ncbi_nrp_id": "WP_012855378.1", "uniref100_id": "UniRef100_D1J7N8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20B1", "RefSeq:WP_012855378.1", "UniRef:UniRef100_D1J7N8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7N8", "uniref90_id": "UniRef90_D1J7N8" }, "psc": { "uniref90_id": "UniRef90_D1J7N8", "gene": "rpsT", "product": "30S ribosomal protein S20", "uniref50_id": "UniRef50_Q98PR3", "cog_id": "COG0268", "cog_category": "J", "kegg_orthology_id": "K02968", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PR3" ], "product": "Small ribosomal subunit protein bS20" }, "genes": [ "rpsT" ], "id": "GLJLFKCOHC_263", "locus": "GCA013368035_00228" }, { "type": "cds", "sequence": "contig_1", "start": 134232, "stop": 134507, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTAAACAAGGAACAAGAAATCGTAGCCTATGTGCCACGTAAGTATATGAATACAATAATATTGGATAATTTTTTAAAATCGTCGGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAGAATAAATTTTATTTATGA", "aa": "MVTKKKYDGYLKYSKNNESELINYYLTNNKNIYLKLNKEQEIVAYVPRKYMNTIILDNFLKSSVPKLLEIKTKIEQKKILAIDIEKNKFYL", "aa_hexdigest": "56f9b88474e4b9eb27b636cdc7c7c8a4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10943.76899999999, "isoelectric_point": 9.566286277770995 }, "id": "GLJLFKCOHC_264", "locus": "GCA013368035_00229" }, { "type": "cds", "sequence": "contig_1", "start": 134541, "stop": 134864, "strand": "+", "frame": 3, "gene": "ygjP", "product": "YgjP-like metallopeptidase domain-containing protein", "db_xrefs": [ "COG:COG1451", "COG:R", "SO:0001217", "UniRef:UniRef50_D1J7N9", "UniRef:UniRef90_D1J7N9" ], "nt": "ATGTTAAATTTATTTGAATTAAATAAGAATTTAAAAATCTTAAAACAAGGTATAGAATTTACTCTAAATAAATATTTAAAAAAAATACTGCTGGAATTATCAACTTCTTATGTAGAAAAATTTTGTAAGTTATTATGCATACCATCTGAGTCAATAAAAGTAAGAATTTTGAAGAAGAATAATGCCTGGGGAACAAATCATTTTTTAAAAAGAACAATAACTTTTTCAGATCGGTTAATTTTTTACAGAAAAGAAGTTTTGGAATACGTTGCTTGCCATGAAGTTGTTCATTTTATTTATCAAAACCATTCATCTGAATTTTGA", "aa": "MLNLFELNKNLKILKQGIEFTLNKYLKKILLELSTSYVEKFCKLLCIPSESIKVRILKKNNAWGTNHFLKRTITFSDRLIFYRKEVLEYVACHEVVHFIYQNHSSEF", "aa_hexdigest": "bee4b6da76d60fedeb84cc58f1c707af", "start_type": "ATG", "rbs_motif": null, "genes": [ "ygjP" ], "truncated": "both", "pseudogene": { "start": 134241, "stop": 134936, "observations": { "mutation": [ 134505, 134862 ], "stop": [ 134505, 134862 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7N9", "query_cov": 1.0, "subject_cov": 0.45726495726495725, "identity": 0.991, "score": 208.0, "evalue": 2.49e-66, "gene_start": 1, "gene_end": 107, "reference_start": 104, "reference_end": 210, "reference_sequence": "MVTKKKYDGYLKYSKNNESELINYYLTNNKNIYLKLNKEQEIVAYVPRKYMNTIILDNFLKSSVPKLLEIKTKIEQKKILAIDIEKNKFYLWGKESKFLIQKNMLNLFELNKNLKILKQGIEFTLNKYLKKILLELSTSYVEKFCKLLCIPSESIKVRILKKNNAWGTNHFLKRTITFSDRLIFYRKEVLEYVACHEVVHFIYQNHSFEFWNMVKKYIPNFKQIKKELKDKIFI" }, "paralog": false, "identity": 0.987012987012987, "score": 452.0, "evalue": 4.36e-165, "description": "Internal stop codon at 134505, 134862. Nonsense mutation around 134505, 134862.", "psc": { "uniref90_id": "UniRef90_D1J7N9", "gene": "YgjP", "product": "YgjP-like metallopeptidase domain-containing protein", "uniref50_id": "UniRef50_D1J7N9", "cog_id": "COG1451", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7N9", "UniRef:UniRef50_D1J7N9", "COG:COG1451", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7N9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7N9" ], "product": "YgjP-like metallopeptidase domain-containing protein" } }, "id": "GLJLFKCOHC_265", "locus": "GCA013368035_00230" }, { "type": "cds", "sequence": "contig_1", "start": 134949, "stop": 135290, "strand": "+", "frame": 3, "gene": null, "product": "NERD domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0", "UniRef:UniRef90_D1J7P0" ], "nt": "ATGGAAAATAAAACAAAATTTATAAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCAATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAGGTATTTTTAGATTCATACAATACACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCCAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTTTTGTATTAAAGCCTTATATGTATGACTCTGACTCTTCTATTCAAGGTGACTATTTGAGCAACGAATGA", "aa": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKVFLDSYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFVLKPYMYDSDSSIQGDYLSNE", "aa_hexdigest": "6b6c5c3139c1eb78008bb996d920c557", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 134949, "stop": 135587, "observations": { "mutation": [ 135288 ], "stop": [ 135288 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P0", "query_cov": 1.0, "subject_cov": 0.5330188679245284, "identity": 0.973, "score": 193.0, "evalue": 1.35e-60, "gene_start": 1, "gene_end": 113, "reference_start": 1, "reference_end": 113, "reference_sequence": "MENKTKFINGIIIGLVLLLVIVIILISVSIVLSKKNRRFLKDQKGFLDSYNTQLKNISESIKNCKFIGANDYSYNDQRIHLDDIFVTDKIIFILKPYMYDSDSSIQGDYFSNEWILTKSNGKKSKIPNQLISLKKSMNNFGLLFSNEMSICSIFVHNLSTELNIEDLPSFQGIYNVKDLCASIKSVYNSNINSMYNQSVSTIFNVLNNKIAK" }, "paralog": false, "identity": 0.9575471698113207, "score": 354.0, "evalue": 1.63e-126, "description": "Internal stop codon at 135288. Nonsense mutation around 135288.", "psc": { "uniref90_id": "UniRef90_D1J7P0", "product": "NERD domain-containing protein", "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P0", "UniRef:UniRef50_D1J7P0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7P0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7P0" ], "product": "NERD domain-containing protein" } }, "id": "GLJLFKCOHC_266", "locus": "GCA013368035_00231" }, { "type": "cds", "sequence": "contig_1", "start": 135357, "stop": 135587, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTGCATAATTTAAGCACGGAATTAAATATAGAAGATGTGCCATCGTTTCAAGGAATTTGCAATATTAAAGATTTATGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTATTTCAACTATTTTCAATGTTTTAAATAACAAAATTGCAAAATAA", "aa": "MNNFGLLFSNEMSICSIFVHNLSTELNIEDVPSFQGICNIKDLCASIKSVYNSNINSMYNQSISTIFNVLNNKIAK", "aa_hexdigest": "3f4bad52d60dedad36c99758f0851bdc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8510.662199999995, "isoelectric_point": 5.537618446350096 }, "id": "GLJLFKCOHC_267", "locus": "GCA013368035_00232" }, { "type": "cds", "sequence": "contig_1", "start": 135746, "stop": 135934, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAAGAACAAAAAACAGTATTAGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGA", "aa": "MAKEQKTVLEKYYISEAFDKDRNISFNFKKANKGISGNFLNFSSAVEEFIKVSENTQNPTKC", "aa_hexdigest": "c6147096031e44b0c32b36152fe9cf71", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7128.958700000002, "isoelectric_point": 8.787637138366701 }, "id": "GLJLFKCOHC_268", "locus": "GCA013368035_00233" }, { "type": "cds", "sequence": "contig_1", "start": 136058, "stop": 136168, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3B5093", "UniRef:UniRef90_UPI001E3B5093" ], "nt": "TTGGTTGAAAAACCTGCAGCAAAAGCTAAGAAAGTTGAAGAACCAAAACAAGAATGTGAATGCTCATGCTCTTGTGATTGTTTAACCGGTATTGAAAACAACAAACACTGA", "aa": "MVEKPAAKAKKVEEPKQECECSCSCDCLTGIENNKH", "aa_hexdigest": "eb53802b4805d3520c8aa908a76e9c35", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 136058, "stop": 136330, "observations": { "mutation": [ 136166, 136178 ], "stop": [ 136166, 136178 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3B5093", "query_cov": 1.0, "subject_cov": 0.4, "identity": 0.972, "score": 53.9, "evalue": 1.62e-08, "gene_start": 1, "gene_end": 36, "reference_start": 1, "reference_end": 36, "reference_sequence": "MVEKPASKAKKVEEPKQECECSCSCDCLTGIENNKHWKEYWTKKSSNNIVCSGIQDHKGGEKLFITLTVLLSLLTIINLILIILLATNVI" }, "paralog": false, "identity": 0.9333333333333333, "score": 144.0, "evalue": 6.67e-47, "description": "Internal stop codon at 136166, 136178. Nonsense mutation around 136166, 136178.", "psc": { "uniref90_id": "UniRef90_UPI001E3B5093", "uniref50_id": "UniRef50_UPI001E3B5093", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3B5093", "UniRef:UniRef50_UPI001E3B5093" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3B5093", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3B5093" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 3981.555799999999, "isoelectric_point": 5.667097663879395 }, "id": "GLJLFKCOHC_269", "locus": "GCA013368035_00234" }, { "type": "cds", "sequence": "contig_1", "start": 136684, "stop": 136971, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGCGTGAAGTTTTAGGTGAATTAAATATTAAATATAAAGAATTTATTGGAGAAGCTGCATTTTATGGACCTAAGGTTGATTTTCAAGTAAAAACTGCACTAAATAGAATTATTACTATGTCAACCTTACAATTAGACTTTTTATTACCATCAAGATTTGAAATGAAATTTGTTGATAGTAATGAACAATTTGTAACACCAGTATTAATTCATAGAGGTTTAATTGGAACTTATGAAAGATTTATTGCAACACTACTTGAACAAACAAAAGGTGTATTGCCATTTTGA", "aa": "MREVLGELNIKYKEFIGEAAFYGPKVDFQVKTALNRIITMSTLQLDFLLPSRFEMKFVDSNEQFVTPVLIHRGLIGTYERFIATLLEQTKGVLPF", "aa_hexdigest": "f194bf682ba591db6ef0c59e7468d337", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI0029CA135A", "query_cov": 0.8736842105263158, "subject_cov": 0.8217821782178217, "identity": 0.578, "score": 102.0, "evalue": 1.71e-26, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "ec_ids": [ "6.1.1.3" ], "uniref50_id": "UniRef50_Q71HR4", "kegg_orthology_id": "K01868" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 78, "aa_cov": 0.7894736842105263, "hmm_cov": 0.9743589743589743, "evalue": 1.0081127132834475e-14, "score": 50.41576385498047, "start": 14, "stop": 88 } ], "seq_stats": { "molecular_weight": 10973.738299999988, "isoelectric_point": 6.570439720153809 }, "id": "GLJLFKCOHC_270", "locus": "GCA013368035_00235" }, { "type": "cds", "sequence": "contig_1", "start": 136990, "stop": 137358, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "ATGTTTGCCCCAATTAAAATGGATAACGAAACATTAGTTGCTAGACCTATGACTTGCCCACACCATATAATATTATTCAATTCAACAAGACGTTCATACAAAGACTTGCCAATTAGATATTCAGAACAATCAAGGTTATATCGTTACGAAGCATCGGGTGCTTTAAGTGGACTTGAAAGAGTTAGAGGAATGGATTTAACAGAAGGCCACATTTTTGTTAGACCAGATCAAATAAAATCAGAATTTAAGCACTTGTATAAAATGATATTGCAAGCATTAAAAGATTTTAATATTGAAATAGATCATATTTCTTTATCATTAAGAGATCCACAAGATACTCAAAAATTCTTCCAAAATGATGAAATGTGA", "aa": "MFAPIKMDNETLVARPMTCPHHIILFNSTRRSYKDLPIRYSEQSRLYRYEASGALSGLERVRGMDLTEGHIFVRPDQIKSEFKHLYKMILQALKDFNIEIDHISLSLRDPQDTQKFFQNDEM", "aa_hexdigest": "97320dfde981601fae09eb5d9d7d2548", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 104, "aa_cov": 0.8360655737704918, "hmm_cov": 0.9807692307692307, "evalue": 6.649966831233274e-22, "score": 73.80873107910156, "start": 8, "stop": 109 } ], "seq_stats": { "molecular_weight": 14435.421899999981, "isoelectric_point": 6.96768741607666 }, "id": "GLJLFKCOHC_271", "locus": "GCA013368035_00236" }, { "type": "cds", "sequence": "contig_1", "start": 137377, "stop": 137496, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGATTCATAATGCAATTAGAGATTATGTTTTAAAACTTGATAAAAAATTTGGTTTTAGAGAAGTACTAACTCCACACTTTGGTGAAAAGAAGCTTTATGAAATAAGTGGACACTGA", "aa": "MKIHNAIRDYVLKLDKKFGFREVLTPHFGEKKLYEISGH", "aa_hexdigest": "a17315a2b28feeeb87eb514c8fb89092", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4659.415, "isoelectric_point": 9.5220609664917 }, "id": "GLJLFKCOHC_272", "locus": "GCA013368035_00237" }, { "type": "cds", "sequence": "contig_1", "start": 137509, "stop": 137670, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAACAAGAATCTATGGTACTTGTTGGGAAACAAAAGATGAATTAGCAAAATATTTAGATATCTTAAGGGAAAGAAAAGAAAGAGATCATCGTAAAATAGGAAGAGATTTAAATTTATTCACTTTTAACCAATTAGGTGGACAAGGATTCCCATTTTGA", "aa": "MLTRIYGTCWETKDELAKYLDILRERKERDHRKIGRDLNLFTFNQLGGQGFPF", "aa_hexdigest": "c58cc23b31d45c62610ae0c87358d88e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6391.2588, "isoelectric_point": 8.976658439636232 }, "id": "GLJLFKCOHC_273", "locus": "GCA013368035_00238" }, { "type": "cds", "sequence": "contig_1", "start": 137803, "stop": 138120, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGCAAATAAAAAATTAAATCATTCAGCAAGCCATTTATTAGCGGCTGCTATATTAAAACTTTATCCAAATGCTAAATTGGCAATTGGACCAGCCATTGAAGAAGGGTTCTATTACGACTTTGAATTTGAAAATCCAATTTTAGAATCAGATCTTCCAAATATTGAAAAAGTAATGAAAGAATTAGCAAAAGAAGGTTACGAAATGCGAAAAGTTGGCATTGAACAATATTCTTTTGAAAATCAACCATATAAAAAAGAACTTTATGAAGAATTTAAAAAAGAAGGAAAAGAAATAACATTCTTTCAATATTGA", "aa": "MEANKKLNHSASHLLAAAILKLYPNAKLAIGPAIEEGFYYDFEFENPILESDLPNIEKVMKELAKEGYEMRKVGIEQYSFENQPYKKELYEEFKKEGKEITFFQY", "aa_hexdigest": "5b3a71cd39b162d39bb0cc2e71f1f051", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12319.913599999993, "isoelectric_point": 5.108199882507324 }, "id": "GLJLFKCOHC_274", "locus": "GCA013368035_00239" }, { "type": "cds", "sequence": "contig_1", "start": 138120, "stop": 138794, "strand": "-", "frame": 3, "gene": null, "product": "Tryptophan--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "nt": "ATGACGCAATTTAAAGATAAATCAGCTAAAATAAAAACTGCAAATGGTATGGAAACAATTCCAACAGGAATTTTAATGTATCCTACTTTAATGGTTGGCGATATATTACTATATAATGCAGATATTATTCCTGTTGGCATCGACCAAAAGCAACATGTTGAATTGACTAGAAATTTAGCAATTAGATTAAATAATAAATTCAATACAACGTACAAAATACCTGAACCATATATACCAGAAGTTGGGGCCAAAATAATGTCTTTAACTGACCCAAGTAAGAAAATGTCAAAATCTGATACAAATCCTAAATCAGCAATTTATTTATTAGATGATCCAGAAGATGCATACAAAAAAATATTAAAAGCTGTTACAGATTCAGAAGGAAAAATATACTTGTCAGATGAAAAGCCGGGCATAAAAAACCTTTTGACTATATATAGTGCTTTAAATGATATTTCATTACAAGAAAGCGCTGAAAAATTTAAAAACAGCAATTACGGTGAATTTAAAAACGCTGTCGCTCAATGTGTAAAGGAATTTTTAATTAAGATCCAAGATAAGTATAAAAATGCCTTAAATCAAATTGAAACATTGAGTAAAGAGGGAGCAAAAAAGGCAAGTGAAGTTGCCCAAATTAACTTAGATATGTTATTAAAAGGAATAGGATTAAGATAA", "aa": "MTQFKDKSAKIKTANGMETIPTGILMYPTLMVGDILLYNADIIPVGIDQKQHVELTRNLAIRLNNKFNTTYKIPEPYIPEVGAKIMSLTDPSKKMSKSDTNPKSAIYLLDDPEDAYKKILKAVTDSEGKIYLSDEKPGIKNLLTIYSALNDISLQESAEKFKNSNYGEFKNAVAQCVKEFLIKIQDKYKNALNQIETLSKEGAKKASEVAQINLDMLLKGIGLR", "aa_hexdigest": "fd7b8c8172c37627a113216ad05a7c0b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3B0PAD4", "query_cov": 1.0, "subject_cov": 0.9333333333333333, "identity": 0.71, "score": 323.0, "evalue": 1.59e-109, "valid": false, "gene": "trpS", "product": "tryptophan--tRNA ligase", "ec_ids": [ "6.1.1.2" ], "uniref50_id": "UniRef50_A0A3B0PAD4", "go_ids": [ "GO:0000166", "GO:0004830", "GO:0005524", "GO:0006436" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PAD4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PAD4" ], "product": "Tryptophan--tRNA ligase" }, "genes": [], "id": "GLJLFKCOHC_275", "locus": "GCA013368035_00240" }, { "type": "cds", "sequence": "contig_1", "start": 138834, "stop": 139121, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAAATTCTGAAAAGAAGACGTTATTAAGTGGAATTACTGCAACTGGTAAATTAACGCTAGCAAATTATATTGGCGCTATAAAAAATATGGTTAAATTGCAAGAAGAATATAACAATTACATTTTTATAGCAGATTTACATGCTCTAACGTTGCCAATTGATCCTAAGCAATTATCAAAAAATAAAAAGGATATTTTTGCACTTTATATTGCATGTGGAATTGACCCTAAAAAGAGTGTGTTATTTTTTCAATCAGATGTCATTCAACACGGATTAATGAATTGA", "aa": "MQNSEKKTLLSGITATGKLTLANYIGAIKNMVKLQEEYNNYIFIADLHALTLPIDPKQLSKNKKDIFALYIACGIDPKKSVLFFQSDVIQHGLMN", "aa_hexdigest": "a613fab4aa3aa15dabfc15552bb97150", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10644.364899999991, "isoelectric_point": 9.041578102111817 }, "id": "GLJLFKCOHC_276", "locus": "GCA013368035_00241" }, { "type": "cds", "sequence": "contig_1", "start": 139440, "stop": 139676, "strand": "+", "frame": 3, "gene": "ptsG2", "product": "PTS EIIB type-1 domain-containing protein", "db_xrefs": [ "COG:COG1264", "COG:G", "SO:0001217", "UniRef:UniRef50_N9UZL6", "UniRef:UniRef90_D1J7P4" ], "nt": "ATGCAAGATTTAATTTTATATTTGGGTGGAATTTCAAATATAGTTTCTTCATCAGCGACTCAAAAAATTGTAAGAATTTCATTTAAAAATAAAGCAAATATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCAGGTATTGCATTTCAATCTTCAAATATATCATTAGTTGTTGGAAATTGCGCAAATTATTTATCTACATTACTTAATAAGGAATTAAACAATCATGAATAA", "aa": "MQDLILYLGGISNIVSSSATQKIVRISFKNKANISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE", "aa_hexdigest": "bc1f12fee655ac27acbd44e239323904", "start_type": "ATG", "rbs_motif": null, "genes": [ "ptsG2" ], "truncated": "5-prime", "pseudogene": { "start": 139265, "stop": 139676, "observations": { "insertion": [ 139298 ], "stop": [ 139371 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P4", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.987, "score": 146.0, "evalue": 7.59e-44, "gene_start": 1, "gene_end": 78, "reference_start": 59, "reference_end": 136, "reference_sequence": "ISYIGKTIIGEKMLNKNKFLYIFLIIITLGLVLIFWRKKYKILKTKNYLTTDNEITFSMQDLILYLGGISNIVSSSATQKIVRISFKNKTNISIEKIKKLNGISGIAFQSSNISLVVGNCANYLSTLLNKELNNHE" }, "paralog": false, "identity": 0.9632352941176471, "score": 248.0, "evalue": 1.45e-86, "description": "Internal stop codon at 139371. Frameshift due to insertion around 139298.", "psc": { "uniref90_id": "UniRef90_D1J7P4", "gene": "PtsG2", "product": "PTS EIIB type-1 domain-containing protein", "uniref50_id": "UniRef50_N9UZL6", "cog_id": "COG1264", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P4", "UniRef:UniRef50_N9UZL6", "COG:COG1264", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_N9UZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UZL6" ], "product": "PTS EIIB type-1 domain-containing protein" } }, "id": "GLJLFKCOHC_277", "locus": "GCA013368035_00242" }, { "type": "cds", "sequence": "contig_1", "start": 139669, "stop": 140043, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0", "UniRef:UniRef90_UPI0024114FF0" ], "nt": "ATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTATAACAATTTTGGCCAAGAAATATTTGATCAAATTTTCAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAGGAAATAATAACAATTTATAAAGATAATAACGTCAGAATCGAACAATCGATATTCAAGATGCTATCAACTGATTTTGAAAAGGCTAAAACTTTGTTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGA", "aa": "MNNKDSKYQTLMANLLNIDTYDTSLYTSINSENFFDIYNNFGQEIFDQIFKNYKFNCILTEVKNKEMRQLLEKAQNKQEIITIYKDNNVRIEQSIFKMLSTDFEKAKTLLITKLESEFQKSIVN", "aa_hexdigest": "11dc00f925aeb645eb72db61d3602459", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0024114FF0", "query_cov": 1.0, "subject_cov": 0.8157894736842105, "identity": 1.0, "score": 153.0, "evalue": 1.46e-45, "valid": true, "uniref50_id": "UniRef50_UPI0024114FF0" }, "pscc": { "uniref50_id": "UniRef50_UPI0024114FF0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114FF0" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14779.667499999985, "isoelectric_point": 5.516190147399901 }, "id": "GLJLFKCOHC_278", "locus": "GCA013368035_00243" }, { "type": "cds", "sequence": "contig_1", "start": 140098, "stop": 140346, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCCTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCCTTGGTTTATTTGAAATCAAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGTTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATTGAATCTATATTTGAGCATTTAAAAAGAATTTGA", "aa": "MHVGFLFISIKTDKKTIFAPLLFKEVTLEVKNSLVYLKSNSDVRINSKLVTFLSQEGFLLNIDNLDFSNLSIESIFEHLKRI", "aa_hexdigest": "470694e780c225f6cfea60ea59c7d5ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9461.970299999994, "isoelectric_point": 8.050699043273926 }, "id": "GLJLFKCOHC_279", "locus": "GCA013368035_00244" }, { "type": "cds", "sequence": "contig_1", "start": 140362, "stop": 140484, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCTGGATATTTATGA", "aa": "MPQSLRSNIPLVSQSDIQNVSIEFHPGMIFGFYNVSSGYL", "aa_hexdigest": "3b3542bb815f6037943a18df730e216e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4474.0337, "isoelectric_point": 5.3026460647583 }, "id": "GLJLFKCOHC_280", "locus": "GCA013368035_00245" }, { "type": "cds", "sequence": "contig_1", "start": 140491, "stop": 140679, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATTAATACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCCACAGTGTCTGCACTATGTCAAGATACAATAATTTGA", "aa": "MKKIIENDEFESILVPEINKNVYREKINTSIFNNKFKLFKVQKTNFSQDVATVSALCQDTII", "aa_hexdigest": "82aa51f4507ea3369e6fa1a6adb94bd3", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7239.282900000003, "isoelectric_point": 7.844142341613771 }, "id": "GLJLFKCOHC_281", "locus": "GCA013368035_00246" }, { "type": "cds", "sequence": "contig_1", "start": 140761, "stop": 141579, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAAAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAAAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAAGGTTATGTTGATACATTGAATGAAATAGAAGCACTGCCTAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATTTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATATGAAGTAAATTTAAAAAGAAGACCGTCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATCAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTATTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCATTTGCAATGTCTATAAGAACGGGTAGCTTAAAACCTTACATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGA", "aa": "MSQKKAALDVLKNRLGQLSLFCLFALNDKNLRQEVFYEPLKKFIEKIENFSISQVEKGIQIFSDEDKGYVDTLNEIEALPNLNNLLHFYASTLNANLTINDFNTLRLLAKDIKYDVEGHTFNDKKELKKYLYEVNLKRRPSIFSIYPRSIKEASELIINAPELFKINVDEAVKYIDKVEFAELETFVEKYKNLLIKKTINVSNSAVLSNMLFAKLINTISNFNDEQKREYTSFAMSIRTGSLKPYMFFHRYKNIIKVLFPIIVTTPELDLSM", "aa_hexdigest": "9aa8ea5ff1aceeaebe3286e37169acd0", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31698.368200000034, "isoelectric_point": 8.641874122619633 }, "id": "GLJLFKCOHC_282", "locus": "GCA013368035_00247" }, { "type": "cds", "sequence": "contig_1", "start": 141751, "stop": 141960, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGTGATATTGAATCTCTTCTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTTGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAGACCAATTGATGTAATTCAAGTTGATGGCGAATGA", "aa": "MGDIESLLDYATARGTYSILLDKNYRSKRASLMTFSSKHFYDSKLDVIDDFKLAFSSERPIDVIQVDGE", "aa_hexdigest": "88f9d4fb6c1a4b670a1b4e6bd3b74ede", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7884.7716, "isoelectric_point": 4.954848289489745 }, "id": "GLJLFKCOHC_283", "locus": "GCA013368035_00248" }, { "type": "cds", "sequence": "contig_1", "start": 142075, "stop": 142758, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13087.12" ], "nt": "TTGACAAATGCAATATTTAGCGTTGAACCAGAACTAGAAAAAGCCTTATTTTCTGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGACGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGCGGAAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTCAAAGAATGGCTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCAAAATTAAACGAATTATTGAAGGAACTAAATGCTAACAATTTCGAAATTATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGACGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGA", "aa": "MTNAIFSVEPELEKALFSDKITIKNIENIQGDEADLVIMSVVYDKKTALFGTYVARKGGKNALNVAISRAREKIIVVKSIYADDVEINERSTSDMRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMDVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRIS", "aa_hexdigest": "62ad1e8a35138bd2bde679c02ac50b6d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13087.12", "name": "AAA_12", "length": 64, "aa_cov": 0.2775330396475771, "hmm_cov": 0.9375, "evalue": 5.808826814755878e-12, "score": 40.96646499633789, "start": 15, "stop": 77 } ], "seq_stats": { "molecular_weight": 26225.099500000044, "isoelectric_point": 6.436299705505371 }, "id": "GLJLFKCOHC_284", "locus": "GCA013368035_00249" }, { "type": "cds", "sequence": "contig_1", "start": 142798, "stop": 143886, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07", "UniRef:UniRef90_UPI00215D9F07" ], "nt": "TTGATTAAACACGAAGAAGAGTTAAATTCTCAAATTTTATTGTCCGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTCCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTGATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAGAGCCGAATACTCAAAGGACTATGAAAAAGATTTTTTGGTTAATTATAATGATGTTGAATTGGATAATACGCATATTGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGTCATAAAGATTGAAGATGTCACTTCATCATCGGCTAACGATGAATTAAAAGTAATTAATTCTAATGAACAAAAAACTTCTGCAAAAACAGCAAGTGAAGATATTGAATCGGGATTTTCAGTTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACATTTGATGATCTTGATATAAATATAGCACCTGAAAGTGAACAACAAGTTGAAGCTAAACTCAATTCGGATTCAGACATTATTCAAGAAAATTTTCATGAGCAAAACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACCAAAAAATAATGATGTTCAAGGCGCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAATGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGATGAAAACGAAGAAACTTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATATATGAAAAATAAAGATATTGAGCAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTAAACGTGGAGGACAACAATGTCAAAAAATAG", "aa": "MIKHEEELNSQILLSEENNNLNVEEIKENIIENNALKNIKSSQIKQGPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLVNYNDVELDNTHIDQEFHIENEFESTINNSNVIKIEDVTSSSANDELKVINSNEQKTSAKTASEDIESGFSVEDINKENISNLFSNTFDDLDINIAPESEQQVEAKLNSDSDIIQENFHEQNDEYFNLEENNIEEQPKNNDVQGADETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTELNVEDNNVKK", "aa_hexdigest": "7192488b7049d8404c4b6524c892b244", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 142498, "stop": 143886, "observations": { "mutation": [ 142756 ], "stop": [ 142756 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9F07", "query_cov": 1.0, "subject_cov": 0.7111984282907662, "identity": 0.9279999999999999, "score": 620.0, "evalue": 1.78e-220, "gene_start": 1, "gene_end": 362, "reference_start": 148, "reference_end": 509, "reference_sequence": "MRLFKEWLKFLDLSIIEQKNYLFLDNENKKYDEKTQTILLPKVSNLQMAVLAKLNELLKELNANNFEIISNYSIGTKLLDLVLINKISNNMIIGINIDDFKYLPDYRKYLKFKDDFNFLLSKSYPIINISRISWIVENKQILNKIKILIKHEEELNSQILLSEENNNLNVEEIKENIIENNAIKNIKSNQIKQSPELKEIDFENVDDETFEVDQDSNNKIYELNESEVAQFEEFHNKNINHEFNDQTESRAEYSKDYEKDFLINYNDIELDNTHIDQEFHIENEFESTINNSNAVKIEDATSSSANDELKITNSNEQKISAKTASEDIESGFSIEDINKENISNLFSNTFDDLDINMALESEQQVEAKLNYDSGIVQDKLHEQNDEYFNLEENDIEEQQKNNDVQSPDETLKSQLVYIDNNKEVNDKQKSYDFAGLFADENEETSTDVKNMIDMNTAEYNQYMKNKDIEQDEQNKEITKNTADEEILNFDLIEDEKTTEFNVEDNNVKK" }, "paralog": false, "identity": 0.9415584415584416, "score": 863.0, "evalue": 8.42e-319, "description": "Internal stop codon at 142756. Nonsense mutation around 142756.", "psc": { "uniref90_id": "UniRef90_UPI00215D9F07", "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9F07", "UniRef:UniRef50_UPI00215D9F07" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9F07", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9F07" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 42101.39400000003, "isoelectric_point": 4.0821424484252935 }, "id": "GLJLFKCOHC_285", "locus": "GCA013368035_00250" }, { "type": "cds", "sequence": "contig_1", "start": 143948, "stop": 144199, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCATAAGGATATTGGAACTAAAATTGCAAAGCAAAAAAAGATTAAAAAAATTTTGTTCATAACTTTTTCTGTTGCTGGTATTGTTACAATCGTAGGGGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTATATTGATGTTTCATTATATTCGCAAATAAATTCTAAATTTAAAGAATTGTATCTTGATGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGA", "aa": "MHKDIGTKIAKQKKIKKILFITFSVAGIVTIVGATIGVTSRKKMELVTYDDYIDVSLYSQINSKFKELYLDGKQPDKKNYPIT", "aa_hexdigest": "452bfdcc163353388d73eb6cd2dbfb3a", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9428.019099999994, "isoelectric_point": 9.663955879211429 }, "id": "GLJLFKCOHC_286", "locus": "GCA013368035_00251" }, { "type": "cds", "sequence": "contig_1", "start": 144809, "stop": 145066, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAGATTGAAAAAAATGAAAATAATATTTTTATTAAGACGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAA", "aa": "MKKIEKNENNIFIKTLNLEFKSTFYTIVKNKLILKNKSSYEYAIYVPFFTNKDKTIKDGKEIYQPRVFEYSCPITFVNSNYKKFK", "aa_hexdigest": "3c24eca4298e440a1965aeff997f5854", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10267.868399999994, "isoelectric_point": 9.656799888610841 }, "id": "GLJLFKCOHC_287", "locus": "GCA013368035_00252" }, { "type": "cds", "sequence": "contig_1", "start": 145127, "stop": 145654, "strand": "-", "frame": 1, "gene": "def", "product": "peptide deformylase", "db_xrefs": [ "COG:COG0242", "COG:J", "EC:3.5.1.88", "GO:0036211", "GO:0042586", "KEGG:K01462", "SO:0001217", "UniRef:UniRef50_Q98PN3", "UniRef:UniRef90_D1J7P7" ], "nt": "ATGTTTAAATTTGAAGATGTTAAATTAGTTCAATTACCAGAAAAAGTATTGAGAAATAAATCAAAGAACGTTGAACTCCCATTGACCGCAGAAGATGATTTATTAATACAAAAAATGATTTTTCATGTTGATGATAGTCAAACTCCTCAAACAAAATTTAGACCTGCCGTAGGAGTTGCTGCAGTTCAATATGGAATATTGAAAAATATTTTTTATGTAATGGTAAAAGACGATCAAAATAATTATATTTTTAGAGACGCTTTAATAAATCCAATAATGCTTTCACATTCAAACCATAAAATATCATTGCAAGAAGGGGAGGGATGTTTAAGCGTTAAGGAAAGCGATGCAGGTCAAGAAGGTTTTGTATATAGATATTCAAGAGTTGTAATAAAAGCATATTCGTATTTTGAAAAAAAAGAAAAAATTTATGATGTTTCCGGCTATCCGGCAATTGTATTGCAACACGAATATGACCACTTGCAAGGAAAATTATTTATAGACAGAATCAATAAAAATCAACCTTGA", "aa": "MFKFEDVKLVQLPEKVLRNKSKNVELPLTAEDDLLIQKMIFHVDDSQTPQTKFRPAVGVAAVQYGILKNIFYVMVKDDQNNYIFRDALINPIMLSHSNHKISLQEGEGCLSVKESDAGQEGFVYRYSRVVIKAYSYFEKKEKIYDVSGYPAIVLQHEYDHLQGKLFIDRINKNQP", "aa_hexdigest": "4356b018542dd2d3bbac879a184fc64a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7P7", "query_cov": 1.0, "subject_cov": 0.9459459459459459, "identity": 0.9940000000000001, "score": 346.0, "evalue": 2.35e-120, "valid": true, "gene": "def", "product": "peptide deformylase", "ec_ids": [ "3.5.1.88" ], "uniref50_id": "UniRef50_Q98PN3", "cog_id": "COG0242", "cog_category": "J", "kegg_orthology_id": "K01462", "go_ids": [ "GO:0036211", "GO:0042586" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN3" ], "product": "Peptide deformylase" }, "genes": [ "def" ], "id": "GLJLFKCOHC_288", "locus": "GCA013368035_00253" }, { "type": "cds", "sequence": "contig_1", "start": 145933, "stop": 146208, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01595.25" ], "nt": "ATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTATCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTACCAAAATTAATTGCAAAAGCATATCCCGAAAGAGTTATTAGATCAACTTGTTGA", "aa": "MIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTC", "aa_hexdigest": "7766a89e40dcf13447ec1cdff86791b7", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01595.25", "name": "CNNM", "length": 88, "aa_cov": 0.967032967032967, "hmm_cov": 1.0, "evalue": 8.439007439806573e-20, "score": 66.57260131835938, "start": 2, "stop": 89 } ], "seq_stats": { "molecular_weight": 10111.135399999988, "isoelectric_point": 10.17938060760498 }, "id": "GLJLFKCOHC_289", "locus": "GCA013368035_00254" }, { "type": "cds", "sequence": "contig_1", "start": 146302, "stop": 146844, "strand": "+", "frame": 1, "gene": "mpfA", "product": "Hemolysin C", "db_xrefs": [ "COG:COG1253", "COG:PT", "SO:0001217", "UniRef:UniRef50_A0A077L6Q7", "UniRef:UniRef90_D1J7P8" ], "nt": "ATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAATGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGTAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCTGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGGAAGGCCAGGGCACAAATGGGTTTCGTAATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATCATTGAAGAAGTTGTAGGCGAAATATACGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGA", "aa": "MKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTI", "aa_hexdigest": "692cbb311716660370230c394aa8b159", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "mpfA" ], "truncated": "both", "pseudogene": { "start": 146002, "stop": 147051, "observations": { "mutation": [ 146206, 146227, 146842, 146857, 146914, 147040 ], "stop": [ 146206, 146227, 146842, 146857, 146914, 147040 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7P8", "query_cov": 1.0, "subject_cov": 0.4147465437788018, "identity": 1.0, "score": 342.0, "evalue": 3.08e-115, "gene_start": 1, "gene_end": 180, "reference_start": 186, "reference_end": 365, "reference_sequence": "MPEPLSGNYNTLNSASGASTSTGAPWQYILYSIILFLLILFSAIFSASETAYTTVSRAKIENMIEKKQKFAKLIQKQHLFFNRILGTILIANNLVNIASATLFSYVLSLTTMSSGKATIISTAVMTPLIVLFAEILPKLIAKAYPERVIRSTCWFIEALYWIFFPITYPISKIGKKIYITNTEEDMKNLINIAQDEGVLEINESTMAKNVLDLDSSKVSQNYVKLKDIDYLNYKATLQDALNKFKETNYSRLPIEKDGDMIGILLLKDIFFLKRGNIMNYVKTVPKISANSLLSIALEKLRKARAQMGFVIKNNNDDSVIGIITIEDIIEEVVGEIYDEYDDDEQIYEISLERCEARGNVKISTIWKRFEWDEILNLEAPNDEDLNLSNWLKNRVGHELHVNTKYTYEDKIDFQVISLKNKDQKFDIIDIDWSK" }, "paralog": false, "identity": 0.9828080229226361, "score": 654.0, "evalue": 1.87e-240, "description": "Internal stop codon at 146206, 146227, 146842, 146857, 146914, 147040. Nonsense mutation around 146206, 146227, 146842, 146857, 146914, 147040.", "psc": { "uniref90_id": "UniRef90_D1J7P8", "gene": "MpfA", "product": "Hemolysin C", "uniref50_id": "UniRef50_A0A077L6Q7", "cog_id": "COG1253", "cog_category": "PT", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7P8", "UniRef:UniRef50_A0A077L6Q7", "COG:COG1253", "COG:PT" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6Q7" ], "product": "Hemolysin C" } }, "id": "GLJLFKCOHC_290", "locus": "GCA013368035_00255" }, { "type": "cds", "sequence": "contig_1", "start": 147226, "stop": 147666, "strand": "+", "frame": 1, "gene": "rpsF", "product": "30S ribosomal protein S6", "db_xrefs": [ "COG:COG0360", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "KEGG:K02990", "RefSeq:WP_012855389.1", "SO:0001217", "UniParc:UPI0001BC20BC", "UniRef:UniRef100_D1J7P9", "UniRef:UniRef50_B3PN60", "UniRef:UniRef90_D1J7P9" ], "nt": "ATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTACAACAAAGGCAGAACCTCATCTAATGTCTGAATTAACAAGAAAGCTTAATATTGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAG", "aa": "MSNYEIMILTNPKTTDEELQNLVFSVLDKNSSKFERLERNETAYPIKKLTHVNYFLITTKAEPHLMSELTRKLNIDKSVLRSLIINLDSEKGLKPRKVSKKFNKRIPLNRENKKPYVKNNETSKDASEEKKVARKPRTSKVLDEKK", "aa_hexdigest": "1f6220519f0b35fdeb0c010b1e0bdf54", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20BC", "ncbi_nrp_id": "WP_012855389.1", "uniref100_id": "UniRef100_D1J7P9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20BC", "RefSeq:WP_012855389.1", "UniRef:UniRef100_D1J7P9" ] }, "ips": { "uniref100_id": "UniRef100_D1J7P9", "uniref90_id": "UniRef90_D1J7P9" }, "psc": { "uniref90_id": "UniRef90_D1J7P9", "gene": "rpsF", "product": "30S ribosomal protein S6", "uniref50_id": "UniRef50_B3PN60", "cog_id": "COG0360", "cog_category": "J", "kegg_orthology_id": "K02990", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN60" ], "product": "Small ribosomal subunit protein bS6" }, "genes": [ "rpsF" ], "id": "GLJLFKCOHC_291", "locus": "GCA013368035_00256" }, { "type": "cds", "sequence": "contig_1", "start": 147695, "stop": 147853, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00436.31" ], "nt": "ATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGA", "aa": "MNKVFLIGRLSAKPYKNVTPSNVEYSRFTMAIRREYAAPNSEPVVDFVPCVA", "aa_hexdigest": "e3643d89a9a5ba85e9b34a3c195bc11e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00436.31", "name": "SSB", "length": 54, "aa_cov": 1.0, "hmm_cov": 1.0, "evalue": 2.048880093378134e-09, "score": 32.86436462402344, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 5877.7719000000025, "isoelectric_point": 9.426970100402833 }, "id": "GLJLFKCOHC_292", "locus": "GCA013368035_00257" }, { "type": "cds", "sequence": "contig_1", "start": 148013, "stop": 148153, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGA", "aa": "MSENRRKNNNGKEFNIATEESNTIDNNLENNHQSNESNDNPFDQLD", "aa_hexdigest": "27e71f40877bf2643a03d5e9efb89bb6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5352.431800000002, "isoelectric_point": 4.394188499450684 }, "id": "GLJLFKCOHC_293", "locus": "GCA013368035_00258" }, { "type": "cds", "sequence": "contig_1", "start": 148171, "stop": 148470, "strand": "+", "frame": 1, "gene": "rpsR", "product": "30S ribosomal protein S18", "db_xrefs": [ "COG:COG0238", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_012855391.1", "SO:0001217", "UniParc:UPI0001BC20BE", "UniRef:UniRef100_D1J7Q1", "UniRef:UniRef50_D1J7Q1", "UniRef:UniRef90_D1J7Q1" ], "nt": "ATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGCAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCGGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAGCAGAATAA", "aa": "MARIVRKKPFIRKRPCQFCLSKEPVVYIDYKNEEVLSKLVNLQGKILSSRITGTCAKHQRAVALAIKRARFVGILPYIGLVKRESKKETVKTEKVEKAE", "aa_hexdigest": "8ab7f0f5f7af77f919b7787eca37ee67", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20BE", "ncbi_nrp_id": "WP_012855391.1", "uniref100_id": "UniRef100_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20BE", "RefSeq:WP_012855391.1", "UniRef:UniRef100_D1J7Q1" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Q1", "uniref90_id": "UniRef90_D1J7Q1" }, "psc": { "uniref90_id": "UniRef90_D1J7Q1", "gene": "rpsR", "product": "30S ribosomal protein S18", "uniref50_id": "UniRef50_D1J7Q1", "cog_id": "COG0238", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q1" ], "product": "Small ribosomal subunit protein bS18" }, "genes": [ "rpsR" ], "id": "GLJLFKCOHC_294", "locus": "GCA013368035_00259" }, { "type": "cds", "sequence": "contig_1", "start": 148951, "stop": 149250, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E51F6A9", "UniRef:UniRef90_UPI001E51F6A9" ], "nt": "GTGGCCAATATTTCCTTTAAATTTACATATGAAAAAAATTTTCAAAATCCAATTTCGGTTTATGAAACTAATAAATTTAATATGATCAATAGAGTCTTAACGACTTTTTTAGTATTGTATATTTTATTTACTATTGCTTCTATTTCACTGAATATTTATGTTTCAATAATTTTATTCAAAAACAAGTATGCAAAACTCTTTTTACTTTCATTAGCAAGTGTTTTAGTTTCAAGAATAATTGGTATTTTTTGTGGAATCATTTTGCTTTTAAATAAAAGACTTTCAACAAGCAAATGCTAA", "aa": "MANISFKFTYEKNFQNPISVYETNKFNMINRVLTTFLVLYILFTIASISLNIYVSIILFKNKYAKLFLLSLASVLVSRIIGIFCGIILLLNKRLSTSKC", "aa_hexdigest": "a08e0507ff346862d6db205b54f3b23d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E51F6A9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.99, "score": 176.0, "evalue": 7.36e-56, "valid": true, "uniref50_id": "UniRef50_UPI001E51F6A9" }, "pscc": { "uniref50_id": "UniRef50_UPI001E51F6A9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E51F6A9" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11383.621599999982, "isoelectric_point": 9.956319999694824 }, "id": "GLJLFKCOHC_295", "locus": "GCA013368035_00260" }, { "type": "cds", "sequence": "contig_1", "start": 149306, "stop": 150034, "strand": "+", "frame": 2, "gene": "rarA", "product": "ATPase, AAA family", "db_xrefs": [ "COG:COG2256", "COG:L", "KEGG:K07478", "SO:0001217", "UniRef:UniRef50_A0A077L8I4", "UniRef:UniRef90_D1J7Q3" ], "nt": "ATGGATTCTAACTTAGCAAATTTAATAAGGCCAAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTACGGAAAGCCAGGTACTGGAAAAACAACTATTTCCTATATTCTTGCCAATGCCTTGAAAGTAAATTACGAATATTTTAATGCGGCCATTGAAAATAAAGAAGATCTTGTACATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGTTACCATATCTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCTGCATTGCGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGATTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTGATTATTTAGCCTTGCAGGCTAATGGCGACTTTAGATCTGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCGTTGGAGGCAATTAAGAAGATTATTCCTTCAATTCAATTTAGTTCAGATGAATCTGGCGATAATCATTATGATTATTTGAGCGCTTTTCACAAATCACTACGCGGTTCTGACATTGATGCAGCATTATATTGA", "aa": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALY", "aa_hexdigest": "9da47514d867137624f354b080ba7fbc", "start_type": "ATG", "rbs_motif": null, "genes": [ "rarA" ], "truncated": "3-prime", "pseudogene": { "start": 149306, "stop": 150337, "observations": { "mutation": [ 150032 ], "stop": [ 150032 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q3", "query_cov": 1.0, "subject_cov": 0.599009900990099, "identity": 1.0, "score": 482.0, "evalue": 1.43e-169, "gene_start": 1, "gene_end": 242, "reference_start": 1, "reference_end": 242, "reference_sequence": "MDSNLANLIRPKILEDFICPQEQKKLFQKIIENNDYRSFIFYGKPGTGKTTISYILANALKVNYEYFNAAIENKEDLVHKLKLNKILIIDEIHRLNKDKQDILLPYLENDLITIYATTTENPYFKLNPALRSRCSIIEITKPTIQDLSSCLKNACLKTFGVDLNKDIADYLALQANGDFRSALNNLDLIMLIFKDQEFSLEAIKKIIPSIQFSSDESGDNHYDYLSAFHKSLRGSDIDAALYWGFIIIKSGDFDGLFRRMLCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQYWDLIKNKGEK" }, "paralog": false, "identity": 0.9970845481049563, "score": 680.0, "evalue": 4.48e-250, "description": "Internal stop codon at 150032. Nonsense mutation around 150032.", "psc": { "uniref90_id": "UniRef90_D1J7Q3", "gene": "RarA", "product": "ATPase, AAA family", "uniref50_id": "UniRef50_A0A077L8I4", "cog_id": "COG2256", "cog_category": "L", "kegg_orthology_id": "K07478", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q3", "UniRef:UniRef50_A0A077L8I4", "COG:COG2256", "COG:L", "KEGG:K07478" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L8I4" ], "product": "ATPase" } }, "id": "GLJLFKCOHC_296", "locus": "GCA013368035_00261" }, { "type": "cds", "sequence": "contig_1", "start": 150086, "stop": 150490, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12002.13" ], "nt": "TTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCAGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGCTCATCAACATTAATAAAATCTATAATTGATAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATATTGA", "aa": "MCASYEDVGMANPKLGSEVLSAIECFERIGMPEGYLPITQAILNICLSPKSNTVYSSSTLIKSIIDSGNIFEVPIHLKDAHYKSAAKLKRGLNYKYPHDYKNNYIDQQYLPNELKDKIHIELNGQGYEQKIKQY", "aa_hexdigest": "0d568cf3f41a63fd024eea087f9fb425", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12002.13", "name": "MgsA_C", "length": 132, "aa_cov": 0.9850746268656716, "hmm_cov": 1.0, "evalue": 4.253468398445457e-42, "score": 139.26321411132812, "start": 2, "stop": 133 } ], "seq_stats": { "molecular_weight": 15219.24799999998, "isoelectric_point": 6.890670585632326 }, "id": "GLJLFKCOHC_297", "locus": "GCA013368035_00262" }, { "type": "cds", "sequence": "contig_1", "start": 150522, "stop": 150839, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02912.23" ], "nt": "ATGGAATTTGAATTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTTAACAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAAAACTTGGTAAAAAAATTCAACTGCTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACTGGAGGAATTCATCCAATTAATTTGGTTGCAAATAGATTTAGAGAATGA", "aa": "MEFELNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAKLGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFRE", "aa_hexdigest": "8e929f96dd0181aa5f58e20a9cfa8ce5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02912.23", "name": "Phe_tRNA-synt_N", "length": 64, "aa_cov": 0.6, "hmm_cov": 1.0, "evalue": 6.245933666908212e-08, "score": 27.96074676513672, "start": 11, "stop": 73 } ], "seq_stats": { "molecular_weight": 12101.781499999986, "isoelectric_point": 5.876606178283691 }, "id": "GLJLFKCOHC_298", "locus": "GCA013368035_00263" }, { "type": "cds", "sequence": "contig_1", "start": 150843, "stop": 151280, "strand": "+", "frame": 3, "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "db_xrefs": [ "COG:COG0016", "COG:J", "EC:6.1.1.20", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432", "KEGG:K01889", "SO:0001217", "UniRef:UniRef50_C5J6V7", "UniRef:UniRef90_D1J7Q4" ], "nt": "TTGGCAAATGGATATTTTGAATATACAGGGCCAGAAATAGAAAATGAAGAATATAACTTTGAACGTTTGAATATTCCTCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACAGGATTTAGTGCAAGAGCATTAGAGGAACATAAAAATCAAGAGTTCTCACAATTTGCTATTGGAAAAGTTTATAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGCAACCATTCATTTGGAACATTGATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCCTATTTCCCATTTACTGAACCTAGTGTTGAGGTTGATGTTTTGTTTAATGAAAAATGA", "aa": "MANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEK", "aa_hexdigest": "440bb23b0ffb9648cd4f870c67eeb395", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pheS" ], "truncated": "both", "pseudogene": { "start": 150543, "stop": 151463, "observations": { "mutation": [ 150837, 151278 ], "stop": [ 150837, 151278 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q4", "query_cov": 1.0, "subject_cov": 0.46325878594249204, "identity": 0.993, "score": 290.0, "evalue": 6.57e-97, "gene_start": 1, "gene_end": 145, "reference_start": 108, "reference_end": 252, "reference_sequence": "MEFDLNKINTLEDLKLAKNAFNNSDELKELMSQIKTADPLQKAELGKKIQLLKKQAEDFFDLAKQKLEDIKIQDLINQEYVDFANPINTTGGIHPINLVANRFREWLLANGYFEYTGPEIENEEYNFERLNIPQSHPAREMQDSLYISEKELLRTHNTGFSARALEEHKNQEFSQFAIGKVYRNDEEDRTHTHQFTQLDFVSVGNHSFGTLIYTLTELFSYVLEQKVKIRLRPSYFPFTEPSVEVDVLFNEKWVEVLGAGMLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK" }, "paralog": false, "identity": 0.9901960784313726, "score": 602.0, "evalue": 1.36e-222, "description": "Internal stop codon at 150837, 151278. Nonsense mutation around 150837, 151278.", "psc": { "uniref90_id": "UniRef90_D1J7Q4", "gene": "pheS", "product": "phenylalanine--tRNA ligase subunit alpha", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_C5J6V7", "cog_id": "COG0016", "cog_category": "J", "kegg_orthology_id": "K01889", "go_ids": [ "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q4", "EC:6.1.1.20", "UniRef:UniRef50_C5J6V7", "COG:COG0016", "COG:J", "KEGG:K01889", "GO:0000166", "GO:0004826", "GO:0005524", "GO:0005737", "GO:0006432" ] }, "pscc": { "uniref50_id": "UniRef50_C5J6V7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C5J6V7" ], "product": "Phenylalanine--tRNA ligase alpha subunit" } }, "id": "GLJLFKCOHC_299", "locus": "GCA013368035_00264" }, { "type": "cds", "sequence": "contig_1", "start": 151302, "stop": 151463, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01409.25" ], "nt": "ATGTTGCATGAAAATGTTTTGAAAATGGCTGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGACCAAAGATTTTTAAAACAATTTAAATAA", "aa": "MLHENVLKMAGYTNDMNGIAAGIGIERITMIKYGIDDIRELYGNDQRFLKQFK", "aa_hexdigest": "247ce24247d8c02fcba2a60b9e877202", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01409.25", "name": "tRNA-synt_2d", "length": 52, "aa_cov": 0.9811320754716981, "hmm_cov": 1.0, "evalue": 9.303787681183302e-20, "score": 66.50789642333984, "start": 1, "stop": 52 } ], "seq_stats": { "molecular_weight": 6107.0264, "isoelectric_point": 6.533664894104004 }, "id": "GLJLFKCOHC_300", "locus": "GCA013368035_00265" }, { "type": "cds", "sequence": "contig_1", "start": 151475, "stop": 152188, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01588.26" ], "nt": "ATGTTATTTTCTTATAAAAAAATAGTTAAATTAGCAAATATAACTGACAAAAGTGTAGAAGAGGTTGTTGGGGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTCTAGATGCTGAAGGTATGAAATTCTGTCATGTATTAAAAGCATACAAAAATCCTAATTCTGATCGTTTAACGGTTTGTGAAGTTGAATTTGGCAATGGAAATCATTCAATAATTCAAACTAATGCAACAAACATGCATGAGGGCGATTATGTAATGGCTTATGTTCCTGGATCGAGATCTGGAAAAAATATATTTGCTGCCAAGGAATTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCCTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTGAATATTTTGATCTTGATGATTATTTGATTGATGTAACAATTTTATCCAATAGAGCTGATGCTTTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCTTATATTGAACCTCTTCCAAAACCAAAACCTACCTTGGAAAGCAATATTGTTGTAAGCAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCTATTACTAAACAACCTGCTATTTCTCTTCAAGATGAATTTCTTTTATGA", "aa": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLPKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLL", "aa_hexdigest": "acd0738d7a2dfd87ada1f6d3abc0b44b", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001F47B76D", "query_cov": 1.0126582278481013, "subject_cov": 1.00418410041841, "identity": 0.504, "score": 223.0, "evalue": 7.71e-70, "valid": false, "uniref50_id": "UniRef50_A0AAV4HE63" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01588.26", "name": "tRNA_bind", "length": 82, "aa_cov": 0.3291139240506329, "hmm_cov": 0.9146341463414634, "evalue": 1.683796966847959e-09, "score": 33.12977600097656, "start": 46, "stop": 123 } ], "seq_stats": { "molecular_weight": 26471.01180000003, "isoelectric_point": 4.757219123840332 }, "id": "GLJLFKCOHC_301", "locus": "GCA013368035_00266" }, { "type": "cds", "sequence": "contig_1", "start": 152234, "stop": 153613, "strand": "+", "frame": 2, "gene": "eMAP", "product": "phenylalanine--tRNA ligase subunit beta", "db_xrefs": [ "COG:COG0073", "COG:J", "EC:6.1.1.20", "KEGG:K01890", "SO:0001217", "UniRef:UniRef50_A0A448ZZ89", "UniRef:UniRef90_D1J7Q5" ], "nt": "TTGGTCTTATTATACGCAGGAGTTCCTTGTCACGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGTAATGAAACATTAAATGTTTTAGGCAATAAACAGGTTACATTAAACAACAATTTGGTTGTAAAAAACGGAGATCAAACCGTTTCAATAGCGAGTGTTATAGGCTTAGAAAATTATGAATATAGACCTGATGGTGGACATTGCGTATTTGAATTAGCATCATTTTCATTAAAAGAAGTTAGAAAAAGTATAAAAACAGTCAAATTAGAAACATTAGCATCTTTACGTGGTTCAAAAGAAATATCAAGCGGTCAAGTAGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCGCCTAAGCTAAGAAAAACAAATATATTAATTGATAGCCATTATATTACTAAATATGCAGGCTTCAATATTACGAAAACAAAAAAATATATTCAAGTTTTGAATCAATTGACTCTTTTGGGATTTAAAATAAAGGAAGATTTATCATCAGTAATTTTCCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTATTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAATAAGGAATTTAAGTTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTCAATCCATTTGATTTTAAAACTAATTTCAATGCAATTGCTGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTAGATACAAATATCAAACAAGGCTTGCCAGACGCTTCATATTTTGAAATAGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGATATTTGTTCAATAACTAATGAAACATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAGCGAATTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGCGAAGTCTTTTTGGATAAAATGAATAGTAATGTAATTAAATATAAAAATTATAAACATACACCTCTTAAGTCTAGAGATGTTACTATATCTGTTTTAGATAAACAATCTATTGAAGAAACAATTAAAAAAATACAAAGTATTTTTGGCGTTTACAAAATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGA", "aa": "MVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDGGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPDASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNSELIGYVAKLDPHYCQYNAVFSEVFLDKMNSNVIKYKNYKHTPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILED", "aa_hexdigest": "5b01dcec3a3aed30293299edb93dd120", "start_type": "TTG", "rbs_motif": null, "genes": [ "eMAP" ], "truncated": "both", "pseudogene": { "start": 151934, "stop": 153649, "observations": { "mutation": [ 152186, 153611 ], "stop": [ 152186, 153611 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q5", "query_cov": 1.0, "subject_cov": 0.6339779005524862, "identity": 0.996, "score": 898.0, "evalue": 0.0, "gene_start": 1, "gene_end": 459, "reference_start": 254, "reference_end": 712, "reference_sequence": "MLFSYKKIVKLANITDKSVEEVVGAINSIGFEVEEYHKFLDAEGMKFCHVLKAYKNPNSDRLTVCEVEFGNGNHSIIQTNATNMHEGDYVMAYVPGSRSGKNIFAAKELKGIISEGMFVSLNEIGIPEEFHPLDSKDGIFQVGKIDLNLDPVEYFDLDDYLIDVTILSNRADALCYLILAKEISAYFESYIEPLSKPKPTLESNIVVSKNLVATNCFSLVEAITKQPAISLQDEFLLWKHGIKTFRNAVDITNLVLLYAGVPCHVYNKNSLSSNEFSTSLSNETLNVLGNKQVTLNNNLVVKNGDQTVSIASVIGLENYEYRPDGGHCVFELASFSLKEVRKSIKTVKLETLASLRGSKEISSGQVVMAYEFLTKYLDQYSVQINAPKLRKTNILIDSHYITKYAGFNITKTKKYIQVLNQLTLLGFKIKEDLSSVIFPTYRYDLKNMQDFVEEVFRFYGYDNFPAKSPKITRLLIKENKEFKFIENFCAKGYFNVRTYTLINPEKNIFNPFDFKTNFNAIAAKNYDHSQIRNSMISSLLEVLDTNIKQGLPDASYFEIAMINNKMNVLGIASLNKTIDDIKADICSITNETLEFKPSKLKIFNPNAAQEIYLNNELIGYVAKLDPHYCQYNAVFSEVFLDKMNSNVIKYKNYKHTPLKSRDVTISVLDKQSIEETIKKIQSIFGVYKISIKDVYNKDNGTKNITLEVILEDWATKKFDSIFNK" }, "paralog": false, "identity": 0.9929947460595446, "score": 1115.0, "evalue": 0.0, "description": "Internal stop codon at 152186, 153611. Nonsense mutation around 152186, 153611.", "psc": { "uniref90_id": "UniRef90_D1J7Q5", "gene": "EMAP", "product": "phenylalanine--tRNA ligase subunit beta", "ec_ids": [ "6.1.1.20" ], "uniref50_id": "UniRef50_A0A448ZZ89", "cog_id": "COG0073", "cog_category": "J", "kegg_orthology_id": "K01890", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q5", "EC:6.1.1.20", "UniRef:UniRef50_A0A448ZZ89", "COG:COG0073", "COG:J", "KEGG:K01890" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZ89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZ89" ], "product": "Phenylalanine--tRNA ligase beta subunit" } }, "id": "GLJLFKCOHC_302", "locus": "GCA013368035_00267" }, { "type": "cds", "sequence": "contig_1", "start": 153747, "stop": 153932, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTCGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCTGAAACTTTTAGCAGTGGTTCGTTGACTATCGATAGAGCGTTAGGAATAAATGGATGA", "aa": "MKETTNDNLQKEKSIIAETLKEIEKKFGKEAVMILGEKPSIIPETFSSGSLTIDRALGING", "aa_hexdigest": "c9f41f4d8b6240cde5449c81972c0dfd", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6693.649199999999, "isoelectric_point": 5.342262840270995 }, "id": "GLJLFKCOHC_303", "locus": "GCA013368035_00268" }, { "type": "cds", "sequence": "contig_1", "start": 153993, "stop": 154634, "strand": "+", "frame": 3, "gene": "recA", "product": "Protein RecA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "nt": "TTGCACGCAATTGCTGAAATACAAAAAACTGGCGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCCGGAGCAATAGGACTAATCGTTGTTGATTCGGTTGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCTTTAAGAAAAATAACTGGATCATTATCAAAAAACAAGACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAATGGAGACATCACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCATATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAAGGCTCGTGA", "aa": "MHAIAEIQKTGGIAAFIDAEHSIDPIYAKNLGVDIDNLILSQPDSGEQALEIVDTLSKSGAIGLIVVDSVAALVPEAELNGEMKDQVVGAQARLMSKALRKITGSLSKNKTTVIFINQIREKIGVIFGNPETTTGGRALKFYSSIRLEVRKIQNVTNNGDITGNQIKCKVVKNKLAAPYKTAQIDIIFSKGISKELEIINLAEELGILVKKGS", "aa_hexdigest": "b0f1378336773daef7e2d17b28aa8210", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A0D5BUH9", "query_cov": 0.9765258215962441, "subject_cov": 0.9244444444444444, "identity": 0.625, "score": 266.0, "evalue": 9.97e-88, "valid": false, "gene": "recA", "product": "recombinase RecA", "uniref50_id": "UniRef50_A0A7U9RV21", "kegg_orthology_id": "K03553", "go_ids": [ "GO:0003677", "GO:0003697", "GO:0005524", "GO:0006281", "GO:0008094" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9RV21", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9RV21" ], "product": "Protein RecA" }, "genes": [ "recA" ], "id": "GLJLFKCOHC_304", "locus": "GCA013368035_00269" }, { "type": "cds", "sequence": "contig_1", "start": 154800, "stop": 155024, "strand": "+", "frame": 3, "gene": null, "product": "Metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "nt": "ATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCGGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCGGGAAGAAAGGGTTTTGAGCCTAAAGATTACGAAAGATTGAAAAGAGCGGGAATAAATGCATTTACATTAGGAAATCACGTTTGA", "aa": "MKNENIKILFLGDIFGLPGIRFVEQQLPNIIKQNNIDFVIAQAENVSGRKGFEPKDYERLKRAGINAFTLGNHV", "aa_hexdigest": "d1de8a61c0e753d54d0f0f87de7e0890", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBC5", "query_cov": 0.9324324324324325, "subject_cov": 1.0, "identity": 0.536, "score": 89.0, "evalue": 6.56e-22, "valid": false, "product": "Metallophosphoesterase", "uniref50_id": "UniRef50_A0A432FBC5" }, "pscc": { "uniref50_id": "UniRef50_A0A432FBC5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBC5" ], "product": "Metallophosphoesterase" }, "genes": [], "id": "GLJLFKCOHC_305", "locus": "GCA013368035_00270" }, { "type": "cds", "sequence": "contig_1", "start": 155385, "stop": 155606, "strand": "+", "frame": 3, "gene": null, "product": "Putative metallophosphoesterase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "nt": "TTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGCCCAAGAGATTGCGCTATTGGTTCTGACTTCCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAGGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACCAAGGATCCAGCAGAAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAA", "aa": "MPKGTYFVTDAGMCGPRDCAIGSDFQEVYEKMRYAAFSAFKVSKNKCQLNGVIFTLTKDPAEKHIELVNIKEQ", "aa_hexdigest": "8282ca45faaa513dd9bb80f277f0eab0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PKR0", "query_cov": 0.9863013698630136, "subject_cov": 0.9863013698630136, "identity": 0.6809999999999999, "score": 108.0, "evalue": 1.66e-29, "valid": false, "product": "Putative metallophosphoesterase", "uniref50_id": "UniRef50_A0A3B0PKR0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PKR0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PKR0" ], "product": "Putative metallophosphoesterase" }, "genes": [], "id": "GLJLFKCOHC_306", "locus": "GCA013368035_00271" }, { "type": "cds", "sequence": "contig_1", "start": 155608, "stop": 155868, "strand": "+", "frame": 1, "gene": "nusB", "product": "N utilization substance B-like protein(Protein nusB)", "db_xrefs": [ "COG:COG0781", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J7Q8", "UniRef:UniRef90_D1J7Q8" ], "nt": "ATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGGATCTTTCAATACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCGAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATATGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGA", "aa": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKDLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISED", "aa_hexdigest": "b40b5389667a39fa05e4df71e197f566", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nusB" ], "truncated": "3-prime", "pseudogene": { "start": 155608, "stop": 156081, "observations": { "mutation": [ 155866, 155872 ], "stop": [ 155866, 155872 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Q8", "query_cov": 1.0, "subject_cov": 0.5477707006369427, "identity": 0.988, "score": 164.0, "evalue": 1.42e-50, "gene_start": 1, "gene_end": 86, "reference_start": 1, "reference_end": 86, "reference_sequence": "MNNDSSSKISEKDKRILFLLNKYKARMHIINCIYQSELFNKNLSIQEILENCGENQLNANEIESLKIIEAKYDVFTRIAKKFISEDWEWNRILPLSRAIIIYGEYELLFNDPRVVINEMVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN" }, "paralog": false, "identity": 0.9808917197452229, "score": 301.0, "evalue": 8.48e-107, "description": "Internal stop codon at 155866, 155872. Nonsense mutation around 155866, 155872.", "psc": { "uniref90_id": "UniRef90_D1J7Q8", "gene": "NusB", "product": "N utilization substance protein B homolog(Protein nusB)", "uniref50_id": "UniRef50_D1J7Q8", "cog_id": "COG0781", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Q8", "UniRef:UniRef50_D1J7Q8", "COG:COG0781", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Q8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Q8" ], "product": "N utilization substance protein B homolog(Protein nusB)" } }, "id": "GLJLFKCOHC_307", "locus": "GCA013368035_00272" }, { "type": "cds", "sequence": "contig_1", "start": 155962, "stop": 156081, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAG", "aa": "MVNISKIYSPNNDYKFINKVLDLVSKNVIKKNTKDEKEN", "aa_hexdigest": "348a48353fc4b62036a2aff9bc7366ba", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4585.2389, "isoelectric_point": 9.399055290222169 }, "id": "GLJLFKCOHC_308", "locus": "GCA013368035_00273" }, { "type": "cds", "sequence": "contig_1", "start": 156065, "stop": 156799, "strand": "+", "frame": 2, "gene": "yqxC", "product": "Hemolysin A", "db_xrefs": [ "COG:COG1189", "COG:J", "EC:2.1.1.226", "EC:2.1.1.227", "KEGG:K06442", "RefSeq:WP_020002694.1", "SO:0001217", "UniParc:UPI000380FB80", "UniRef:UniRef100_A0A6A8PYQ3", "UniRef:UniRef50_A0A077LAY8", "UniRef:UniRef90_D1J7Q9" ], "nt": "ATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAAATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTTGAAGAAACCGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAAACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGTGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCCTGCGATGTTTCATTTATAAGTTTGAAATATGTATTTGAGGTTTGCAATAAAATATTAAATAATAATATAAATGTAGTTTTTTTAATTAAACCCCAATTTGAAGCTTCAAGCAAATATGTTGAACCCGGAGGATATGTTAATCAAGAAAATCATAAATATATTATTGATAAAGTAATTTCATATGCAAAAAATAATGGCTTTGTTTTGATTGATTTAATAAAAAGCCCTATTTTAGGAGAAAAATCAAAAAATATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAA", "aa": "MKKKISEIITNIFKYDLKISEALIREGRVIVNNEKIIIPSLKFEETDVDIRFINMKLPYVSRGAYKLLEAIEKFNINLTDKICLDIGSSTGGFVDVMLRHGAKKVYAVDCGTNQLAYELRINDKVVVYEKTNLKNLKKEMFLENIEFVSCDVSFISLKYVFEVCNKILNNNINVVFLIKPQFEASSKYVEPGGYVNQENHKYIIDKVISYAKNNGFVLIDLIKSPILGEKSKNIEYLGFFRKDS", "aa_hexdigest": "c2e03aa06980103e2b607a2345db1b05", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000380FB80", "ncbi_nrp_id": "WP_020002694.1", "uniref100_id": "UniRef100_A0A6A8PYQ3", "db_xrefs": [ "SO:0001217", "UniParc:UPI000380FB80", "RefSeq:WP_020002694.1", "UniRef:UniRef100_A0A6A8PYQ3" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PYQ3", "uniref90_id": "UniRef90_D1J7Q9" }, "psc": { "uniref90_id": "UniRef90_D1J7Q9", "gene": "YqxC", "product": "Hemolysin A", "ec_ids": [ "2.1.1.226", "2.1.1.227" ], "uniref50_id": "UniRef50_A0A077LAY8", "cog_id": "COG1189", "cog_category": "J", "kegg_orthology_id": "K06442" }, "pscc": { "uniref50_id": "UniRef50_A0A077LAY8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LAY8" ], "product": "Hemolysin A" }, "genes": [ "yqxC" ], "id": "GLJLFKCOHC_309", "locus": "GCA013368035_00274" }, { "type": "cds", "sequence": "contig_1", "start": 156799, "stop": 157491, "strand": "+", "frame": 1, "gene": "csdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "db_xrefs": [ "COG:COG0520", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZVE8", "UniRef:UniRef90_D1J7R0" ], "nt": "ATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGATATTGTATATTTCGATAATTCAGCACTTACTTTTAAACCACAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGTGTTTTAGGAATCCAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACTATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTTAAAAACAATAATATAACATTTAAATATGCAAAAGACAACGAGGAAGTTCTAGATTTAATAAATGAAAAAACAAGGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTGAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCTTATTTGATAAATGATGCCGCACAGGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGCAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAGAAAAAATAGTTCCTCAAAAATGA", "aa": "MNYKDLFPMFKNNPDIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVMHETRKKIADLIDASADQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTRLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLEKIVPQK", "aa_hexdigest": "ff01b4bc2dd358c096e313240909c5ed", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "csdA" ], "truncated": "3-prime", "pseudogene": { "start": 156799, "stop": 157794, "observations": { "mutation": [ 157489, 157531 ], "stop": [ 157489, 157531 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R0", "query_cov": 1.0, "subject_cov": 0.5912596401028277, "identity": 0.987, "score": 446.0, "evalue": 1.04e-155, "gene_start": 1, "gene_end": 230, "reference_start": 1, "reference_end": 230, "reference_sequence": "MNYKDLFPMFKNNPGIVYFDNSALTFKPQTVINSGIDFYEKYSISTRTADSVLGIQTEEVIHETRKKIADLIDASANQTIFTSGTTDGLNLISLMLKDIIKDGEIIFSYFNHSSAIVPFIENLKNNNITFKYAKDNEEVLDLINEKTRLIVLPQLTNNFNVFFDLKKIHQKSKKYGAYLINDAAQAIAHEKVSLNDCDVIVFSSNKIYGPTGMGALVIEKDLLEKIVPQKWGGGQVQSIGSGCEWSAKNDIKKFEPGTPNFAGIFQFNAAIDFINSLGYELINKLETAVANYCYDELKTIDGIEIDSKRGDKIILFNIKNIPSQDIASYLGHNNIYVRSGVFCAHKFHELKQYSNSYVRISISFYNDYNDVDKFIKLLKEMGANFLEFL" }, "paralog": false, "identity": 0.9848942598187311, "score": 644.0, "evalue": 2.32e-236, "description": "Internal stop codon at 157489, 157531. Nonsense mutation around 157489, 157531.", "psc": { "uniref90_id": "UniRef90_D1J7R0", "gene": "CsdA", "product": "Nitrogen fixation protein NifS(Aminotransferase protein S)", "uniref50_id": "UniRef50_A0A448ZVE8", "cog_id": "COG0520", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R0", "UniRef:UniRef50_A0A448ZVE8", "COG:COG0520", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZVE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZVE8" ], "product": "Probable cysteine desulfurase" } }, "id": "GLJLFKCOHC_310", "locus": "GCA013368035_00275" }, { "type": "cds", "sequence": "contig_1", "start": 157955, "stop": 158404, "strand": "+", "frame": 2, "gene": "iscU", "product": "Aminotransferase protein U-like protein (Nitrogenfixation protein NifU)", "db_xrefs": [ "COG:COG0822", "COG:O", "RefSeq:WP_020002692.1", "SO:0001217", "UniParc:UPI0003650E82", "UniRef:UniRef100_A0A6A8PZC5", "UniRef:UniRef50_D1J7R1", "UniRef:UniRef90_D1J7R1" ], "nt": "TTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGATGATATTAAGCTAAATTTTTTATTTGATGAAAAAAATACTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCCGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATGAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGTGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAA", "aa": "MNFYSNIEKQRIIFEAYENPKFKIKNDEDFKKTDKTLFEHSNVCVDDIKLNFLFDEKNTLKKVEYCAKGCSIFLSSVELMIQQILNKNKLEINEILNEYKTMIDTSTISQRGGEILGKLVVYQNVRVHLNRLECASIIFRLIKKAVSNE", "aa_hexdigest": "8b4703889b764e3cc14019fde846bcfe", "start_type": "TTG", "rbs_motif": "GGxGG", "ups": { "uniparc_id": "UPI0003650E82", "ncbi_nrp_id": "WP_020002692.1", "uniref100_id": "UniRef100_A0A6A8PZC5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003650E82", "RefSeq:WP_020002692.1", "UniRef:UniRef100_A0A6A8PZC5" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PZC5", "uniref90_id": "UniRef90_D1J7R1" }, "psc": { "uniref90_id": "UniRef90_D1J7R1", "gene": "IscU", "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)", "uniref50_id": "UniRef50_D1J7R1", "cog_id": "COG0822", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_D1J7R1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R1" ], "product": "Aminotransferase protein U homolog (Nitrogenfixation protein NifU)" }, "genes": [ "iscU" ], "id": "GLJLFKCOHC_311", "locus": "GCA013368035_00276" }, { "type": "cds", "sequence": "contig_1", "start": 158397, "stop": 159290, "strand": "+", "frame": 3, "gene": "dinP", "product": "DNA-damage repair protein MucB", "db_xrefs": [ "COG:COG0389", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J7R2", "UniRef:UniRef90_D1J7R2" ], "nt": "ATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACCTGATAGACAAGCCTGTTGTAATTGCTAAAAGATACAAACGTTCAATAATTAGTGCAATGTCTTACGAGGTAAAAAACCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTACTTATAGTAGAACCACACTATGATTTATATTCTCTAATTTCTAAAAAAATTTTTGATTATTTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTATTAGCAATTGGGAGGAAGCTAAAAATCTTGCCCAACAAATTAAAGATGATATTTTAAAAACATTTATGATTCCTTGTTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTTAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCGCAACAATTAATAAAAAATAAAATTTCTACTTATAAAGATTTGCTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATTAATGCAACCGACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGA", "aa": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTISNWEEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKEDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKK", "aa_hexdigest": "48cdb1fe800b637f7322e036074a5a2d", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [ "dinP", "mucB" ], "truncated": "3-prime", "pseudogene": { "start": 158397, "stop": 159593, "observations": { "mutation": [ 159288, 159375 ], "stop": [ 159288, 159375 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7R2", "query_cov": 1.0, "subject_cov": 0.7105263157894737, "identity": 0.99, "score": 584.0, "evalue": 1e-208, "gene_start": 1, "gene_end": 297, "reference_start": 1, "reference_end": 297, "reference_sequence": "MNKIIFHIDMDSFFVSCERSINTNLIDKPVVIAKRYKRSIISAMSYEVKNLGYRVGSSLYTVLEKVPNLLIVEPHYDLYSLISKKIFDYLKNKYCTQLEIYSIDECYIDFSNTVSNWAEAKNLAQQIKDDILKTFMIPCSIGISFTKFLAKMSTNKAKPSGILITKKGDIERNFYSLPVEKIFGVGKNISQQLIKNKISTYKDLLECNNEIFLRKLFKKNFFLFIEQLKGINATDHILNLETKGISNTITFMEKDSDDIVFLKNELFKLARNVANRAVNNNYECRCVEVLIRKQDKKWMSEQKKIDFFTNDIDIIFKIALKLFLNLWNEKECLRGLGIRICDLRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK" }, "paralog": false, "identity": 0.9849246231155779, "score": 773.0, "evalue": 6.47e-286, "description": "Internal stop codon at 159288, 159375. Nonsense mutation around 159288, 159375.", "psc": { "uniref90_id": "UniRef90_D1J7R2", "gene": "DinP", "product": "DNA-damage repair protein MucB", "uniref50_id": "UniRef50_D1J7R2", "cog_id": "COG0389", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7R2", "UniRef:UniRef50_D1J7R2", "COG:COG0389", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7R2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7R2" ], "product": "DNA-damage repair protein MucB" } }, "id": "GLJLFKCOHC_312", "locus": "GCA013368035_00277" }, { "type": "cds", "sequence": "contig_1", "start": 159423, "stop": 159653, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGTCGATATTTCAAGAAAACAAACAAGTTAATTTGTTTGAAGACAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATACAAAATTTGAGGGTAAAATTTTAATAACAGGGAAACAATATTTGTTAGAGAATTCAATTGATATTGATAATATAAAGTTTTTAAGAAAAAATAATCAACCCTATGATAAAAAAATAAATTTAGAGGAGAAAAAATAA", "aa": "MRSIFQENKQVNLFEDNNQKSIVNSILNEVNTKFEGKILITGKQYLLENSIDIDNIKFLRKNNQPYDKKINLEEKK", "aa_hexdigest": "53be2b029bc8566ca5d7d8503c3332c4", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9030.19379999999, "isoelectric_point": 8.925663948059086 }, "id": "GLJLFKCOHC_313", "locus": "GCA013368035_00278" }, { "type": "cds", "sequence": "contig_1", "start": 159653, "stop": 159994, "strand": "+", "frame": 2, "gene": null, "product": "nicotinate-nucleotide adenylyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "nt": "ATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCTGTTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTGGATAAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAAGATAAGCAAGATTATGCATCTAATGAACATAGAATAAATATGATTAATATTGTTATAAACAAGAGTAATATGGAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAATGTTGGCAGTTTAAACAAATGA", "aa": "MKIGIFGGSFDPVHKGHILIANDAIELLKLDKVIFVPANKNPFKDKQDYASNEHRINMINIVINKSNMEVSQFETKRGGTSYTIDTVKYFAQKYPNDELYFLIGSDNVGSLNK", "aa_hexdigest": "f144102cbb877a03a0a68a6aa7a7316c", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A432FBE0", "query_cov": 1.008849557522124, "subject_cov": 1.0178571428571428, "identity": 0.588, "score": 130.0, "evalue": 2.63e-37, "valid": false, "gene": "nadD", "product": "nicotinate (nicotinamide) nucleotide adenylyltransferase", "ec_ids": [ "2.7.7.18" ], "uniref50_id": "UniRef50_A0A432FBE0", "go_ids": [ "GO:0000309", "GO:0004515", "GO:0005737", "GO:0009435" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBE0" ], "product": "nicotinate-nucleotide adenylyltransferase" }, "genes": [], "id": "GLJLFKCOHC_314", "locus": "GCA013368035_00279" }, { "type": "cds", "sequence": "contig_1", "start": 160168, "stop": 160356, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTCACTCTTTAGTAATATCATGCATAAAACCAGTTCTATACGCTAATTCGACATCAAAATTATTTCTTTTAGCCAATTGTGCAGAAAATTCAGCAGTATATCTTAAATGTTTAAATCTAGGAATAGAAAGAGTATTTTTGGCAATATTTTCTAAATATAAACAATTCTTTCCTATGTATTCTTGA", "aa": "MSHSLVISCIKPVLYANSTSKLFLLANCAENSAVYLKCLNLGIERVFLAIFSKYKQFFPMYS", "aa_hexdigest": "8c6032f08446de5a4be592873d9b2f5f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7027.318199999999, "isoelectric_point": 9.183988761901855 }, "id": "GLJLFKCOHC_315", "locus": "GCA013368035_00280" }, { "type": "cds", "sequence": "contig_1", "start": 160418, "stop": 160582, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTACACCAAACTACGGCCTATTATTGGTTGAGGGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTAAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGA", "aa": "MLHQTTAYYWLRDVYKYQNDEALQAIKIHTSLDFKISLLAKILYIADKICEGRK", "aa_hexdigest": "bd5763502d0e2b7603ea2617461d9da3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6434.442399999998, "isoelectric_point": 8.788862037658692 }, "id": "GLJLFKCOHC_316", "locus": "GCA013368035_00281" }, { "type": "cds", "sequence": "contig_1", "start": 160801, "stop": 161490, "strand": "+", "frame": 1, "gene": "infC", "product": "translation initiation factor IF-3", "db_xrefs": [ "COG:COG0290", "COG:J", "GO:0003743", "GO:0006413", "RefSeq:WP_020002689.1", "SO:0001217", "UniParc:UPI0003616783", "UniRef:UniRef100_UPI0003616783", "UniRef:UniRef50_A0A448ZXX5", "UniRef:UniRef90_D1J7R4" ], "nt": "TTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATTAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTACAGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAACAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAAAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAATAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACGAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTCCGCGGTAGAGAAAGAGCAAGAATTGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAA", "aa": "MLGCYNKEFVVFGKWINTTFLFIKEGGVIINNQKIGDDLNNNQTNRKPKSEHVINNAIPYKKVFVLGPTGEKIGVLTKEEALEKASEYNMDLVLIAIENNKPIVRILDYGKFKYDKKKRQKEVKEKQAVTVNREIRLTPLIGDHDLKTKANKTREFILDGNRVKVSVKFRGRERARIELGEEILKKFFALIEDVAKISKEATLVNDRFLDMYVEKDKRKVDTLTNKKDN", "aa_hexdigest": "19f4657fcbf6cdce778424ebb9cdfd02", "start_type": "TTG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003616783", "ncbi_nrp_id": "WP_020002689.1", "uniref100_id": "UniRef100_UPI0003616783", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003616783", "RefSeq:WP_020002689.1", "UniRef:UniRef100_UPI0003616783" ] }, "ips": { "uniref100_id": "UniRef100_UPI0003616783", "uniref90_id": "UniRef90_D1J7R4" }, "psc": { "uniref90_id": "UniRef90_D1J7R4", "gene": "infC", "product": "translation initiation factor IF-3", "uniref50_id": "UniRef50_A0A448ZXX5", "cog_id": "COG0290", "cog_category": "J", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXX5" ], "product": "Translation initiation factor IF-3" }, "genes": [ "infC" ], "id": "GLJLFKCOHC_317", "locus": "GCA013368035_00282" }, { "type": "cds", "sequence": "contig_1", "start": 161517, "stop": 161705, "strand": "+", "frame": 3, "gene": "rpmI", "product": "50S ribosomal protein L35", "db_xrefs": [ "COG:COG0291", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_020002688.1", "SO:0001217", "UniParc:UPI00036BF9D9", "UniRef:UniRef100_A0A2K9YT13", "UniRef:UniRef50_P75447", "UniRef:UniRef90_D1J7R5" ], "nt": "ATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAG", "aa": "MPKMKTKSGLKKRISITASGKVKRGQAFRSHLAQNKTTKQKRHARKATTLSSSDFKRYKELI", "aa_hexdigest": "12cf694fb7c510f41385e8bf4b0e3d29", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00036BF9D9", "ncbi_nrp_id": "WP_020002688.1", "uniref100_id": "UniRef100_A0A2K9YT13", "db_xrefs": [ "SO:0001217", "UniParc:UPI00036BF9D9", "RefSeq:WP_020002688.1", "UniRef:UniRef100_A0A2K9YT13" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT13", "uniref90_id": "UniRef90_D1J7R5" }, "psc": { "uniref90_id": "UniRef90_D1J7R5", "gene": "rpmI", "product": "50S ribosomal protein L35", "uniref50_id": "UniRef50_P75447", "cog_id": "COG0291", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P75447", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P75447" ], "product": "Large ribosomal subunit protein bL35" }, "genes": [ "rpmI" ], "id": "GLJLFKCOHC_318", "locus": "GCA013368035_00283" }, { "type": "cds", "sequence": "contig_1", "start": 161976, "stop": 162077, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAA", "aa": "MLVNRKMLSELAINYPTSFAKLVDQAKSALAKK", "aa_hexdigest": "30ec095d133853a43f24cd1b5e60831b", "start_type": "GTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3680.3854, "isoelectric_point": 9.997450828552246 }, "id": "GLJLFKCOHC_319", "locus": "GCA013368035_00284" }, { "type": "cds", "sequence": "contig_1", "start": 162176, "stop": 162286, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02540.23", "PFAM:PF03054.22" ], "nt": "ATGAAAAAAAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGCTACAGAAACAAGGATATGACGTTGTTGGTTTGTTCATGAGAAATTGA", "aa": "MKKRVVLGMSGGVDSSVCGYLLQKQGYDVVGLFMRN", "aa_hexdigest": "09399c4b20bbd243650b061f6885b900", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02540.23", "name": "NAD_synthase", "length": 23, "aa_cov": 0.6388888888888888, "hmm_cov": 1.0, "evalue": 1.290902835531314e-07, "score": 26.389699935913086, "start": 3, "stop": 25 }, { "id": "PF03054.22", "name": "tRNA_Me_trans", "length": 34, "aa_cov": 0.9444444444444444, "hmm_cov": 1.0, "evalue": 4.482436545110024e-12, "score": 41.443851470947266, "start": 3, "stop": 36 } ], "seq_stats": { "molecular_weight": 3936.6466, "isoelectric_point": 9.514002418518068 }, "id": "GLJLFKCOHC_320", "locus": "GCA013368035_00285" }, { "type": "cds", "sequence": "contig_1", "start": 162572, "stop": 163288, "strand": "+", "frame": 2, "gene": null, "product": "tRNA-uridine 2-sulfurtransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "nt": "ATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAGTCTTACTTTTTAGCGCAATTAAGCCCTGACCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTACGAGAAATTGCAAAACAATTAAACTTGTCAACCGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACATTTCACGAAATTTTTAGAAAATTATATTCCTGCTCAACCGGGTAATATCATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCGATGTATTACACTATAGGTCAAAGAAAAGGTTTAAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGACCATATTGACTATTTAATGTCAAATAAATTAATCGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAAATTTTAAATGATAATAAAATAGAAGTTTGCTATCCAAATACCGAAGCAGTAACTCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTAGTTGGTGGAGCGATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAA", "aa": "MGHYANAIDGELFRAQDDNKDQSYFLAQLSPDQLKKVIFPLANITKPEVREIAKQLNLSTAMKKDSTGICFIGERHFTKFLENYIPAQPGNIIDIFSGKVVGKHIGAMYYTIGQRKGLNLGGYEKPYFVVGHNLSKKEIYVTNDDHIDYLMSNKLIATNFNQISKEFNFANLTAKFRYRQKDIKITLKILNDNKIEVCYPNTEAVTPGQQVVIYDGNKVVGGAIIDKIYLNNELKTFL", "aa_hexdigest": "155f4a0071eb37c5e2bc042639942d12", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0030845807", "query_cov": 0.8235294117647058, "subject_cov": 0.98989898989899, "identity": 0.515, "score": 196.0, "evalue": 4.26e-60, "valid": false, "product": "aminomethyltransferase beta-barrel domain-containing protein", "uniref50_id": "UniRef50_A0A380E4E9" }, "pscc": { "uniref50_id": "UniRef50_A0A380E4E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A380E4E9" ], "product": "tRNA-uridine 2-sulfurtransferase" }, "genes": [], "id": "GLJLFKCOHC_321", "locus": "GCA013368035_00286" }, { "type": "cds", "sequence": "contig_1", "start": 163566, "stop": 163712, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTTATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGA", "aa": "MLGNFSIGDYFKKEAIAFGYEFIFNVLKLNKQNIYITYYSEDKDTYNY", "aa_hexdigest": "ee4df62ca45ebba9d68af99bee4f848e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5793.464500000002, "isoelectric_point": 4.992987251281736 }, "id": "GLJLFKCOHC_322", "locus": "GCA013368035_00287" }, { "type": "cds", "sequence": "contig_1", "start": 164004, "stop": 164882, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCAACAGAAAAATTAACAAACAAACATTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTTTAATAAGAAGAGCATATAGGGCAGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATCTATGACATTGATGTTGAAGAAGTTAAAAAAGTTATTTTGGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGCGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACTATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTACAAGAAAAAAACATTGACATCGATATGAATGAATTTAACGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCTGGAATGCAAAAGGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTTGGATATGATTACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGCGAAGAAAAAATAGATAATGCGGGCGAAGATGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGAAATAACAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGA", "aa": "MQDAPTNFDTDLFLPIIHATEKLTNKHYDINNYFTKEPIQMKINTQFKIIADHIRASVNAISDGVKPSNIGRGYIIRRLIRRAYRAGISLGITQKAFLYELVQIVKDSLIYDIDVEEVKKVILDEENLFAKTINEGEKLLNQEIEKNGTIDVKIAFKMFDTYGFPIELTREILQEKNIDIDMNEFNEFLKQHQEKSRGNIEAGMQKVINSLSLITDKISEFVGYDYTKTTSKILYLLNSEEKIDNAGEDEVSYLILDKTPFYATSGGQNHDEGYILQGNNRIEVLDVFKDKY", "aa_hexdigest": "fd6647f814f0b47a49b1b85c1760b44c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 33640.81930000003, "isoelectric_point": 5.085918998718261 }, "id": "GLJLFKCOHC_323", "locus": "GCA013368035_00288" }, { "type": "cds", "sequence": "contig_1", "start": 164994, "stop": 165968, "strand": "+", "frame": 3, "gene": null, "product": "Alanine--tRNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "nt": "ATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAGCAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCAGTCTTGATCATAGGCCAACTAAAGAAGAGATAAAATTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATAAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTATAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTTACTTCATATGATTTAGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAGAAATAATCAAAAACTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAATGCGAAGAACAACTAAGAAATGATTATCGAACATTATTGAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATCTCATTAAGAGAAGCAAATCCTGATTCAATTATTATTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGCGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAACAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAG", "aa": "MFSALRETYPNEIIKQLGSNITYEHFTFDFSLDHRPTKEEIKLIEHKMRQYIARDVKRNYIITSLKKAEEMGAYMTIDESEYHDANYIRVVEFEGITKDLCGGTHVPHSGIIEDFKIVAVDSKGTGIYRLRVVTSYDLVRKYLLSEIQEKQKILDTLIRNNQKLNEGYNFKYTKEIISNKTSDLEELLEEYDKCEEQLRNDYRTLLKQKQSEDVDLSLYNYETINDKKVFLLINIEQSLIKKVSISLREANPDSIIIILNKSKQDSYYFAVASKKYDSNAIFKNLGKVLNIHGGGNAIISQGSLVSNLKQEEIIEIIKSNLCEF", "aa_hexdigest": "104a0751bed0f5ed29ad58dcc80cb91e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZR4", "query_cov": 0.9907407407407407, "subject_cov": 0.9042253521126761, "identity": 0.5579999999999999, "score": 350.0, "evalue": 8.36e-117, "valid": false, "product": "Alanine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0NZR4" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZR4" ], "product": "Alanine--tRNA ligase" }, "genes": [], "id": "GLJLFKCOHC_324", "locus": "GCA013368035_00289" }, { "type": "cds", "sequence": "contig_1", "start": 165956, "stop": 166303, "strand": "+", "frame": 2, "gene": null, "product": "Putative pre-16S rRNA nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "nt": "ATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAACCATTTTAACTTAGTTATTGAAAAAGTGCTACATTATTTAAATAAAAGTGAATATCAAGGTTCAATTGGTACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACATATATGGTCGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAGTTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAGAGCGCTGGATGA", "aa": "MRILALDLGTKSCGFAISDPNNIIASGLENFRFELNHFNLVIEKVLHYLNKSEYQGSIGTIVLGYPLRMDLSKSQRTYMVERFGERLKKVVDIPVVFQDERQSTIGAQEILQSAG", "aa_hexdigest": "6985cc6d5db1c0ac36561d19f3492d67", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00112C3C79", "query_cov": 1.0, "subject_cov": 0.8098591549295775, "identity": 0.687, "score": 160.0, "evalue": 9.65e-49, "valid": false, "gene": "ruvX", "product": "Holliday junction resolvase RuvX", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_B3PM70", "go_ids": [ "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM70" ], "product": "Putative pre-16S rRNA nuclease" }, "genes": [], "id": "GLJLFKCOHC_325", "locus": "GCA013368035_00290" }, { "type": "cds", "sequence": "contig_1", "start": 166383, "stop": 166694, "strand": "+", "frame": 3, "gene": null, "product": "DUF1292 domain-containing protein", "db_xrefs": [ "RefSeq:WP_012855410.1", "SO:0001217", "UniParc:UPI0001BC20F6", "UniRef:UniRef100_D1J7S0", "UniRef:UniRef50_D1J7S0", "UniRef:UniRef90_D1J7S0" ], "nt": "ATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACAATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGATGGAAAATCAGATTTAATGAAGAAATTAATTGGCAACGATCAGGGTGAAAATGAAGCATAA", "aa": "MNKNIINLDVVDRQLTTSDGEKLYVIFDIEENGEHYLVLTDYDAIIFAKEQDQNLIEVTDEGEIDILVDLTMEFAENNFVLDKDGKSDLMKKLIGNDQGENEA", "aa_hexdigest": "85120146fd2db4f4c4dc4a506d74e8d6", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC20F6", "ncbi_nrp_id": "WP_012855410.1", "uniref100_id": "UniRef100_D1J7S0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F6", "RefSeq:WP_012855410.1", "UniRef:UniRef100_D1J7S0" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S0", "uniref90_id": "UniRef90_D1J7S0" }, "psc": { "uniref90_id": "UniRef90_D1J7S0", "product": "DUF1292 domain-containing protein", "uniref50_id": "UniRef50_D1J7S0" }, "pscc": { "uniref50_id": "UniRef50_D1J7S0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S0" ], "product": "DUF1292 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_326", "locus": "GCA013368035_00291" }, { "type": "cds", "sequence": "contig_1", "start": 166684, "stop": 167403, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036438913.1", "SO:0001217", "UniParc:UPI000513DB43", "UniRef:UniRef100_A0A454C9Y1", "UniRef:UniRef50_D1J7S1", "UniRef:UniRef90_D1J7S1" ], "nt": "ATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACTATTTTAGCACGTAGACATTTCTTAAAAAGCACAATAACATCAAGCGAAGAACGAGAACTACAAAATTTAAAAACAAAGAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGATAATGTTTTAGGCGACCATTTGATTGCGTTTTTAATTAATACAATATATTTGAATAATTTTCTAAACGTTAGTCTTATTGAAAAAAATGACTATGCTTCAATTTCAATAGCAGCGTTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCAGGATTTAGATCTATCTAGATTAAATTATGTTGATGAAATTTATAATCCTTCGGATATGATAATATCTTTATGCGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAA", "aa": "MKHKGSIFSANTKLGLIISVVVVFILMCVILLITILARRHFLKSTITSSEERELQNLKTKNPNYGIVLSGIKKYYDNVLGDHLIAFLINTIYLNNFLNVSLIEKNDYASISIAALSMANVYSKNQYLIQKQGSNIQNENQDLDLSRLNYVDEIYNPSDMIISLCDDLSIEQQINSNLPYLSDRGMLIVSFKTIKDFKNQKNVILDNNLRYETVKISNKNVILLAKNNMNSNNENSKEQQ", "aa_hexdigest": "0b74cc363f8abc1bcdfcdd71c70f7054", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000513DB43", "ncbi_nrp_id": "WP_036438913.1", "uniref100_id": "UniRef100_A0A454C9Y1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513DB43", "RefSeq:WP_036438913.1", "UniRef:UniRef100_A0A454C9Y1" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9Y1", "uniref90_id": "UniRef90_D1J7S1" }, "psc": { "uniref90_id": "UniRef90_D1J7S1", "uniref50_id": "UniRef50_D1J7S1" }, "pscc": { "uniref50_id": "UniRef50_D1J7S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S1" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27261.107100000027, "isoelectric_point": 8.68165111541748 }, "id": "GLJLFKCOHC_327", "locus": "GCA013368035_00292" }, { "type": "cds", "sequence": "contig_1", "start": 167403, "stop": 167873, "strand": "+", "frame": 3, "gene": "greA", "product": "transcription elongation factor GreA", "db_xrefs": [ "COG:COG0782", "COG:K", "GO:0003677", "GO:0032784", "GO:0070063", "RefSeq:WP_012855412.1", "SO:0001217", "UniParc:UPI0001BC20F8", "UniRef:UniRef100_D1J7S2", "UniRef:UniRef50_B3PM73", "UniRef:UniRef90_D1J7S2" ], "nt": "ATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAACGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAA", "aa": "MANNDNKIYLTEESLKQYQEKLKYLQEVARPQVIEEIKEARNQGDLSENAEYDSARDKQAAIENEILEIQHILENVEIIKSTSTHTIRIGSTVKLININTNEISEVKIVGALDADPFNNKISNTSPLAIALNGKKVGQVIEVEAPKKYKVKIESIL", "aa_hexdigest": "64b98e0388441a4943fb2bf3806ee693", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC20F8", "ncbi_nrp_id": "WP_012855412.1", "uniref100_id": "UniRef100_D1J7S2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20F8", "RefSeq:WP_012855412.1", "UniRef:UniRef100_D1J7S2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S2", "uniref90_id": "UniRef90_D1J7S2" }, "psc": { "uniref90_id": "UniRef90_D1J7S2", "gene": "greA", "product": "transcription elongation factor GreA", "uniref50_id": "UniRef50_B3PM73", "cog_id": "COG0782", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0032784", "GO:0070063" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM73", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM73" ], "product": "Transcription elongation factor GreA" }, "genes": [ "greA" ], "id": "GLJLFKCOHC_328", "locus": "GCA013368035_00293" }, { "type": "cds", "sequence": "contig_1", "start": 167978, "stop": 168652, "strand": "+", "frame": 2, "gene": "trmN6", "product": "DNA methylase", "db_xrefs": [ "COG:COG4123", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A448ZXG4", "UniRef:UniRef90_D1J7S3" ], "nt": "ATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTACTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAACAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGTGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAACTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGCAATAAATTAGCCAAAAAATATGATTTAATTGTTGCCAATCCACCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACTCATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGCTAAGAAAATACAATTTTGAGCCAAAGAGAATTCAATTAGTTTATCCAAGAATCACCGATCTGCCAAAATTCTGTTTAGTCGAAGCTAGATTTAATTCAGGTTGA", "aa": "MKNEVVKNNLGYSGDLFVYQDKSMFNYSVDTILLGNFVSINRKVRNILEVGTNNGALSIFIAARDKRLNIDAIEIQSKAVELARKNIILNKLENQINLIEGDFRMWANQKAYDCGNKLAKKYDLIVANPPYYNEDYNQQRKNSTLEQKLATHEINLNLEQLVANSARIIEQKGYLAIVLPMARYIDLICLLRKYNFEPKRIQLVYPRITDLPKFCLVEARFNSG", "aa_hexdigest": "1bdc4b0dbaa34b36576f4f70f66f78e0", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S3", "query_cov": 1.0, "subject_cov": 0.8615384615384616, "identity": 1.0, "score": 442.0, "evalue": 1.38e-156, "valid": true, "gene": "TrmN6", "product": "DNA methylase", "uniref50_id": "UniRef50_A0A448ZXG4", "cog_id": "COG4123", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXG4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXG4" ], "product": "DNA methylase" }, "genes": [ "trmN6" ], "id": "GLJLFKCOHC_329", "locus": "GCA013368035_00294" }, { "type": "cds", "sequence": "contig_1", "start": 168783, "stop": 169034, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "nt": "ATGAATAATAAAAAAACTTTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTAACCACTACATTAGCTTCAGTATATAGAAATTACAAAAATCAACAAGGATATGAAACCTTGTTTATTACTGGAATTGATGAACATGGTCAAAAGATTCAAAAAAAAGCTCTTGAACAAAATATTACTCCAAAAGAATACGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGA", "aa": "MNNKKTFYITTPIYYPSGNLHIGHLLTTTLASVYRNYKNQQGYETLFITGIDEHGQKIQKKALEQNITPKEYVDIQSQKFVDL", "aa_hexdigest": "6b7b2cb10f32e4296ae65f98b0501380", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00283F72E3", "query_cov": 0.8795180722891566, "subject_cov": 0.8588235294117647, "identity": 0.603, "score": 93.6, "evalue": 2.16e-23, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A7X9AHZ7" }, "pscc": { "uniref50_id": "UniRef50_A0A7X9AHZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7X9AHZ7" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "GLJLFKCOHC_330", "locus": "GCA013368035_00295" }, { "type": "cds", "sequence": "contig_1", "start": 169119, "stop": 169307, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACAAAAAGGCTATATTTATAAATCTAAATATGAAGGCTTATATTCAATTAGCGATGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGACGGAAAATTTTACCACCCAATTTCAGGACATGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACTCCTTGA", "aa": "MEQKGYIYKSKYEGLYSISDEEFLTKTQAISKDGKFYHPISGHELQEVKEESYFFNMSKFTP", "aa_hexdigest": "44f2d0256d7935fe5526ee4f0eb1c69b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7354.149999999999, "isoelectric_point": 5.585135841369628 }, "id": "GLJLFKCOHC_331", "locus": "GCA013368035_00296" }, { "type": "cds", "sequence": "contig_1", "start": 169371, "stop": 169487, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATTTTATTAATAAAGGATTAGAGGATCTTTCGATAACAAGAGTTTCTTTTGACTGGGGAATTAAAATAGAAACTAAAGATGCTGAAAAGCAACACATTATTTATGTTTGA", "aa": "MNNFINKGLEDLSITRVSFDWGIKIETKDAEKQHIIYV", "aa_hexdigest": "2a966a0ca5c85685c2a5f258d4edcb14", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4467.0631, "isoelectric_point": 5.564105415344237 }, "id": "GLJLFKCOHC_332", "locus": "GCA013368035_00297" }, { "type": "cds", "sequence": "contig_1", "start": 169713, "stop": 170111, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATCAAAAGGCAATGTTATTGAGCCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGACAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTGGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTTCAAACCAATTGAATTCATTAGATAAAAATGTTTATTCAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAGGCTTTAAAAAGGGCAATTGAATTAAGTAAGTTTTTAAATGAATATATTGATAAAAATGAACCTTGA", "aa": "MSKSKGNVIEPIPLIEKYGAEPVKYFFASQINIDNDFSFSEELLINVLNADLANNFGNLANRTFKMVMLNFENGTTYCSNQLNSLDKNVYSEIENAYNDYKKYFDNFNADKALKRAIELSKFLNEYIDKNEP", "aa_hexdigest": "75025e337a5353f3901e80d0fb1d920c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15236.911499999984, "isoelectric_point": 4.756821250915528 }, "id": "GLJLFKCOHC_333", "locus": "GCA013368035_00298" }, { "type": "cds", "sequence": "contig_1", "start": 170127, "stop": 170345, "strand": "+", "frame": 3, "gene": null, "product": "Methionyl-tRNA synthetase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "nt": "TTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAGCATTTTATTAAACGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTCTAAATTTGATAACAAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAA", "aa": "MPRLNVVLNTLLNGIYAVNMMLSVVLKEKCSILLNDLQLSEFSEKMLFNYSKFDNKKFSKPNILFERIKPNN", "aa_hexdigest": "f971750417cbdd3102c05c026e7c4d0d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0PIV2", "query_cov": 0.9444444444444444, "subject_cov": 0.9066666666666666, "identity": 0.5, "score": 62.8, "evalue": 1.61e-11, "valid": false, "product": "Methionyl-tRNA synthetase", "uniref50_id": "UniRef50_A0A3B0PIV2" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PIV2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PIV2" ], "product": "Methionyl-tRNA synthetase" }, "genes": [], "id": "GLJLFKCOHC_334", "locus": "GCA013368035_00299" }, { "type": "cds", "sequence": "contig_1", "start": 170366, "stop": 171517, "strand": "+", "frame": 2, "gene": "obgE", "product": "GTPase ObgE", "db_xrefs": [ "COG:COG0536", "COG:DL", "GO:0003924", "SO:0001217", "UniRef:UniRef50_Q4A6N4", "UniRef:UniRef90_D1J7S5" ], "nt": "ATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGATGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGCGGTCCTGATGGTGGCGATGGTGGCGATGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTCGCTGAATCAGGAGAAAATGGAAGGCCAAAAAATCAATATGGGGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAGGTTTTAGCAGATGTTGGCTTTGTTGGAAAACCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCCGATTTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGCCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGCGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAATAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAAAGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGATGCTTGTTTGAAACATTGCAATCTGCTAAAGATTCAATCGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAACCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGTGATGATATAGATAATATATATAATAAGATACCAGTGATTTCATTAGATAATTTATGA", "aa": "MKFIDETNVIVIAGKGGDGIISFRREANVDKGGPDGGDGGDGGDVYFVGDPGQNTLLPFYYQNKLVAESGENGRPKNQYGADGQDLIVKVPLGTMVYNGDDLIADVVSQEKYLIAKGGHGGKGNPRFKSSTNTAPRICENGTPGQKFELHLVLKVLADVGFVGKPSAGKSTILGEISNAKPKIADYDFTTLVPQLGLVKYFDNSYVVADLPGLIENAHLGKGLGIQFLKHIERCKIIAHVIDFGSEEKNPIDDYEIINNELKSYNLLLEKLPQVVIANKKDLPAFSLNLSKFKEKYPNIKVFEYSALMAENLDELKGCLFETLQSAKDSIAVQQIHESQNIVKITLNKKPIQIKKIDNETYELFGDDIDNIYNKIPVISLDNL", "aa_hexdigest": "e5cd9ea1dbd364c980ce98c147e02431", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7S5", "query_cov": 1.0, "subject_cov": 0.9033018867924528, "identity": 0.995, "score": 740.0, "evalue": 5.77e-269, "valid": true, "gene": "obgE", "product": "GTPase ObgE", "uniref50_id": "UniRef50_Q4A6N4", "cog_id": "COG0536", "cog_category": "DL", "go_ids": [ "GO:0003924" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N4" ], "product": "GTPase Obg" }, "genes": [ "obgE" ], "id": "GLJLFKCOHC_335", "locus": "GCA013368035_00300" }, { "type": "tRNA", "sequence": "contig_1", "start": 171787, "stop": 171863, "strand": "+", "gene": "trnP", "product": "tRNA-Pro(tgg)", "amino_acid": "Pro", "anti_codon": "tgg", "score": 86.0, "nt": "GGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCA", "db_xrefs": [ "SO:0000268" ], "anti_codon_pos": [ 171804, 171806 ], "id": "GLJLFKCOHC_7", "locus": "GCA013368035_00301" }, { "type": "tRNA", "sequence": "contig_1", "start": 171869, "stop": 171945, "strand": "+", "gene": "trnA", "product": "tRNA-Ala(tgc)", "amino_acid": "Ala", "anti_codon": "tgc", "score": 78.5, "nt": "GGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCA", "db_xrefs": [ "SO:0000254" ], "anti_codon_pos": [ 171898, 171900 ], "id": "GLJLFKCOHC_8", "locus": "GCA013368035_00302" }, { "type": "tRNA", "sequence": "contig_1", "start": 171950, "stop": 172026, "strand": "+", "gene": "trnM", "product": "tRNA-Met(cat)", "amino_acid": "Met", "anti_codon": "cat", "score": 77.9, "nt": "GGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCA", "db_xrefs": [ "SO:0000266" ], "anti_codon_pos": [ 171984, 171986 ], "id": "GLJLFKCOHC_9", "locus": "GCA013368035_00303" }, { "type": "tRNA", "sequence": "contig_1", "start": 172051, "stop": 172126, "strand": "+", "gene": "trnI", "product": "tRNA-Ile2(cat)", "amino_acid": "Ile2", "anti_codon": "cat", "score": 86.1, "nt": "GGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCA", "db_xrefs": [ "SO:0000263" ], "id": "GLJLFKCOHC_10", "locus": "GCA013368035_00304" }, { "type": "tRNA", "sequence": "contig_1", "start": 172129, "stop": 172219, "strand": "+", "gene": "trnS", "product": "tRNA-Ser(tga)", "amino_acid": "Ser", "anti_codon": "tga", "score": 74.7, "nt": "GGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 172148, 172150 ], "id": "GLJLFKCOHC_11", "locus": "GCA013368035_00305" }, { "type": "tRNA", "sequence": "contig_1", "start": 172226, "stop": 172301, "strand": "+", "gene": "trnfM", "product": "tRNA-fMet(cat)", "amino_acid": "fMet", "anti_codon": "cat", "score": 69.0, "nt": "AGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCA", "db_xrefs": [ "SO:0000266" ], "id": "GLJLFKCOHC_12", "locus": "GCA013368035_00306" }, { "type": "tRNA", "sequence": "contig_1", "start": 172303, "stop": 172379, "strand": "+", "gene": "trnD", "product": "tRNA-Asp(gtc)", "amino_acid": "Asp", "anti_codon": "gtc", "score": 74.0, "nt": "GGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCA", "db_xrefs": [ "SO:0000256" ], "anti_codon_pos": [ 172304, 172306 ], "id": "GLJLFKCOHC_13", "locus": "GCA013368035_00307" }, { "type": "tRNA", "sequence": "contig_1", "start": 172381, "stop": 172456, "strand": "+", "gene": "trnF", "product": "tRNA-Phe(gaa)", "amino_acid": "Phe", "anti_codon": "gaa", "score": 76.9, "nt": "GGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCA", "db_xrefs": [ "SO:0000267" ], "anti_codon_pos": [ 172414, 172416 ], "id": "GLJLFKCOHC_14", "locus": "GCA013368035_00308" }, { "type": "cds", "sequence": "contig_1", "start": 172543, "stop": 172683, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04193.19" ], "nt": "ATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGA", "aa": "MNINSIKIAIQLFGIFGSIVTICLGIPQLIRLKKTKNTGKVSFAAF", "aa_hexdigest": "fa5f71c74dafc2ab4e51ccf2c18d046b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04193.19", "name": "PQ-loop", "length": 35, "aa_cov": 0.7608695652173914, "hmm_cov": 1.0, "evalue": 3.15313884034939e-07, "score": 25.503488540649414, "start": 10, "stop": 44 } ], "seq_stats": { "molecular_weight": 5009.071300000001, "isoelectric_point": 10.595137214660646 }, "id": "GLJLFKCOHC_336", "locus": "GCA013368035_00309" }, { "type": "cds", "sequence": "contig_1", "start": 172840, "stop": 173094, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAATAAGGGCTATTGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGCCTATTTTTTGTTACGGTATATCAAAAGTTGAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCTGTTCTTCCGGGTAATGCGCAACTTGCATTTGGACTAATATGTCCCGCCCTTACAAGCGCAGCATTTTTCCCTGGATTAATTGCTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGA", "aa": "MLIRAIVGISINQVISLIFLCLFFVTVYQKLKFGKFYPDNSIIPVLPGNAQLAFGLICPALTSAAFFPGLIASLIKKEEISLSP", "aa_hexdigest": "5b132a650ec66af2213dc6b79542e5f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9144.971199999994, "isoelectric_point": 9.138925361633301 }, "id": "GLJLFKCOHC_337", "locus": "GCA013368035_00310" }, { "type": "cds", "sequence": "contig_1", "start": 173360, "stop": 173716, "strand": "-", "frame": 1, "gene": "yIH1", "product": "Impact N-terminal domain-containing protein", "db_xrefs": [ "COG:COG1739", "COG:R", "RefSeq:WP_230588788.1", "SO:0001217", "UniParc:UPI000B272C52", "UniRef:UniRef100_A0A454C9C9", "UniRef:UniRef50_D1J7S7", "UniRef:UniRef90_D1J7S7" ], "nt": "ATGGCAATTTTAGAAATTAAAAAGTCTAAATTTATTTCTTATAAATTTAATATCAAAAGTAAAGATGATGTGAAACTTTTAAAAGAGCAATTAAAAAATCAACATCAAAAAGCAAGGCATATAGTTTATGCTTATTTAGTTATTGAAAATGGCGTTCAATGTGGTGGAATGAGTGATGATGGTGAACCATCAGGAACAGCAGGCAAACCTTTATATTTTTTACTAGAAAGAAATAAATTAAATAATTGTGTGTTGTTTGTAGTTAGATATTTTGGTGGCATAAAATTAGGCGCTAGCAAATTAATTAGAGCATATTCTAAAGTTGCAAATGATGAAATTAAAAGTAGCAATCAGTAA", "aa": "MAILEIKKSKFISYKFNIKSKDDVKLLKEQLKNQHQKARHIVYAYLVIENGVQCGGMSDDGEPSGTAGKPLYFLLERNKLNNCVLFVVRYFGGIKLGASKLIRAYSKVANDEIKSSNQ", "aa_hexdigest": "f4342fd5f2bce06067aee48b618c5dc8", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000B272C52", "ncbi_nrp_id": "WP_230588788.1", "uniref100_id": "UniRef100_A0A454C9C9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000B272C52", "RefSeq:WP_230588788.1", "UniRef:UniRef100_A0A454C9C9" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9C9", "uniref90_id": "UniRef90_D1J7S7" }, "psc": { "uniref90_id": "UniRef90_D1J7S7", "gene": "YIH1", "product": "Impact N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S7", "cog_id": "COG1739", "cog_category": "R" }, "pscc": { "uniref50_id": "UniRef50_D1J7S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S7" ], "product": "Impact N-terminal domain-containing protein" }, "genes": [ "yIH1" ], "id": "GLJLFKCOHC_338", "locus": "GCA013368035_00311" }, { "type": "cds", "sequence": "contig_1", "start": 173757, "stop": 174650, "strand": "+", "frame": 3, "gene": "rluA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG0564", "COG:J", "EC:5.4.99.23", "KEGG:K06180", "RefSeq:WP_012855418.1", "SO:0001217", "UniParc:UPI0001BC20FE", "UniRef:UniRef100_D1J7S8", "UniRef:UniRef50_Q4A5G9", "UniRef:UniRef90_D1J7S8" ], "nt": "ATGTTAAAAATTATTTCAAATTATTCAGACAGAATAGATAAATATGTTGCTAATAATAGCGAGATTTCAAGAAACGATATTAAGGAATTAATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAATAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAGGTTGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGATTAGTTGTTCATCCGGCTCCTGGACATCATGATAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGTATGGGGGTTGTTCACAGAATCGATAAAGATACTAGCGGTTTATTATTAATTGCTAAAAATAATAAAGCGCATAACTACTTTGCTTCGCTTTTAAAAACACATGAAATTAAAAGGAGTTATTACGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCCGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCGTTAGTTAAATGTAATTTAAAAACTGGTCGTACTCATCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGCGATCCAATTTATGGTCACAAAATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAA", "aa": "MLKIISNYSDRIDKYVANNSEISRNDIKELIENGAVFVNSIKINKPKFVVKEGDEILIERLLDKQIKVEPQNIHLDIVYECDDYLIINKPSGLVVHPAPGHHDNTLVNALMYHFKNNLSDVNGLLRMGVVHRIDKDTSGLLLIAKNNKAHNYFASLLKTHEIKRSYYAIVEGKIANEELHIELPIGRDTKNRQKYAVSETNSKPAYTIVNVVKYFKMNNKDFSLVKCNLKTGRTHQIRVHLAYIKHPVYGDPIYGHKIDDFNQRLHAFALDFVDLNGNNKHFEAEMPEIMKKEITGE", "aa_hexdigest": "c66bfbcb51e14849c3255465f1b3c238", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC20FE", "ncbi_nrp_id": "WP_012855418.1", "uniref100_id": "UniRef100_D1J7S8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC20FE", "RefSeq:WP_012855418.1", "UniRef:UniRef100_D1J7S8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7S8", "uniref90_id": "UniRef90_D1J7S8" }, "psc": { "uniref90_id": "UniRef90_D1J7S8", "gene": "RluA", "product": "Pseudouridine synthase", "ec_ids": [ "5.4.99.23" ], "uniref50_id": "UniRef50_Q4A5G9", "cog_id": "COG0564", "cog_category": "J", "kegg_orthology_id": "K06180" }, "pscc": { "uniref50_id": "UniRef50_Q4A5G9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5G9" ], "product": "Pseudouridine synthase" }, "genes": [ "rluA" ], "id": "GLJLFKCOHC_339", "locus": "GCA013368035_00312" }, { "type": "cds", "sequence": "contig_1", "start": 175195, "stop": 175674, "strand": "+", "frame": 1, "gene": null, "product": "Rho termination factor N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9", "UniRef:UniRef90_D1J7S9" ], "nt": "GTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTGTTTTTAAAATGTTCTTATGTTGCAAAATTATTTTTAATTTTAAAGAGAAATTTAGCATTTAAGGCATTAAACAACTCGGGCTCAATGGATGATATTTTAAGATCATATTTCTACGGTCAAGAATCTCCTGAACCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGAAGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAGATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCTAAAAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAAATCAACAATCAAAACCTGATAATTCCGATGAAGACAACAACGATGGAAGTCAAACCAGCAATTAA", "aa": "MATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPEPTLNTNNTFDSNQKTEENKSEDLNKEEDSRSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKPDNSDEDNNDGSQTSN", "aa_hexdigest": "e50e99cf5645eb93ffff504a2484fcdb", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 174895, "stop": 175674, "observations": { "mutation": [ 175021, 175168 ], "stop": [ 175021, 175168 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7S9", "query_cov": 0.9937106918238994, "subject_cov": 0.49221183800623053, "identity": 0.975, "score": 240.0, "evalue": 5.53e-77, "gene_start": 2, "gene_end": 159, "reference_start": 164, "reference_end": 321, "reference_sequence": "MKSININEYNSTNELYTKNKKVFWAWFIAWSVLFATILIFVIASFIQFAVEKQNYLNKYAELFGQIQKLNPNFQGDVFALANNEYVKSLAMSGVLLVILIAVYVWHIVSIALVAKNKDFSAYSRILTTLYLFVIIYEIITLIFSGFQSFSLAIWQVNKILNLVATLLYIVSYFAVFLKCSYVAKLFLILKRNLAFKALNNSGSMDDILRSYFYGQESPESTLNTNNTFDSNQKTEENKSEDLNKEEDSKSIEYRSKLEQLNDEQLQNMAKKLNIFGIETFSREQLISKISDIFEQSAKSQNQQSKPDNSDEDNNDGSQTSN" }, "paralog": false, "identity": 0.9768339768339769, "score": 443.0, "evalue": 3.32e-159, "description": "Internal stop codon at 175021, 175168. Nonsense mutation around 175021, 175168.", "psc": { "uniref90_id": "UniRef90_D1J7S9", "product": "Rho termination factor N-terminal domain-containing protein", "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7S9", "UniRef:UniRef50_D1J7S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7S9" ], "product": "Rho termination factor N-terminal domain-containing protein" } }, "id": "GLJLFKCOHC_340", "locus": "GCA013368035_00313" }, { "type": "cds", "sequence": "contig_1", "start": 175717, "stop": 175812, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATTCTCCTGCTAGTCCAATAAAAAATGGTGATCAAAAATTATGA", "aa": "MNNQTSLKQHIFTFFSQNSPASPIKNGDQKL", "aa_hexdigest": "06f405e4343d81d7761327974f8a8dfb", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3521.910500000001, "isoelectric_point": 9.700380516052245 }, "id": "GLJLFKCOHC_341", "locus": "GCA013368035_00314" }, { "type": "cds", "sequence": "contig_1", "start": 176101, "stop": 176556, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGATTGCGCTTTCAATTGTTTTAATAATATCAATTAATTTATTACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATTCAAAGAATTAAATGATTTAAATGCTCCTGAAAATATTAAACTGGATATCAAAGCAATGGAATCACACAACCCATTAGCCCCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAGTATTGAATTATTTAAACGTTATAAGAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCTCTTGAAGTAATTGAAGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATCTAGACAACATAAATAATAAAGATCCTGAATTGCAAAAAAATTCTCTAATTTTAAAAGAAATTGCTTGA", "aa": "MIALSIVLIISINLLLLAIYFVSFKEAKFVNKKNLEDELISLKYKKFKELNDLNAPENIKLDIKAMESHNPLAPRVLAKFIDFYNKMSSIELFKRYKNYLNQTFKIRYFNESLEVIEGKNEMEDNLEKYLDNINNKDPELQKNSLILKEIA", "aa_hexdigest": "c0527262440a58f67217896a21127220", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17811.621399999996, "isoelectric_point": 7.786249732971191 }, "id": "GLJLFKCOHC_342", "locus": "GCA013368035_00315" }, { "type": "cds", "sequence": "contig_1", "start": 176557, "stop": 176709, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATATGGATAAAAAGGTAAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATACGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGA", "aa": "MNDMDKKVKIIREADKNITMSKKEFQEKYDEYVYLQRSQDPAYQQTQKNT", "aa_hexdigest": "01d8f0b425674717c14c6c03c8d4da74", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6119.825400000004, "isoelectric_point": 7.9425855636596685 }, "id": "GLJLFKCOHC_343", "locus": "GCA013368035_00316" }, { "type": "cds", "sequence": "contig_1", "start": 176902, "stop": 177501, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "RefSeq:WP_175949815.1", "SO:0001217", "UniParc:UPI00158F3401", "UniRef:UniRef100_UPI00158F3401", "UniRef:UniRef50_D1J7T1", "UniRef:UniRef90_D1J7T1" ], "nt": "ATGGCTATAAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCGGTAGTAGTTGTACCTGCAACTATGGTTTCGTGTTCAAAAAAAGATATTGAAGAAATAAAATTAGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAAAAATTTAGAAGTAGAATTTAATAAAGTTATAATTTCGTTAAAAAAAATGGATATAACACCGGATTTTAATATGGACCAATTTATTCAAGAAATGACTAAAAAATTCAGCGATGTAGCAAATAATTTTTTGAATTTAAAATACAATAAAGCCAAATTGACAAGTGAATATTATATTTCAAAAGTAAAAGTTCAGTTAATTTTAGAACAATTTAAAAATCCCGGTTTGTTTCCAGAAAAAAATGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCAACTATTAAAACCTCTGAAGAAGAGTTAAAAAATTCAATTAGATTTTTAGATGATTTAAACAAAAAATATGATGAAATTTTAAAAAAGCAAGTAATGATTTTAAAATAA", "aa": "MAIKHKRILFLSTLALPVVVVPATMVSCSKKDIEEIKLDMITIAKNQMLNYLQKFESFFKNDDDPDVKNLEVEFNKVIISLKKMDITPDFNMDQFIQEMTKKFSDVANNFLNLKYNKAKLTSEYYISKVKVQLILEQFKNPGLFPEKNEFAKILEQELSKVSDPTIKTSEEELKNSIRFLDDLNKKYDEILKKQVMILK", "aa_hexdigest": "f7197e17c1aa606f8c245563664e6d77", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158F3401", "ncbi_nrp_id": "WP_175949815.1", "uniref100_id": "UniRef100_UPI00158F3401", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158F3401", "RefSeq:WP_175949815.1", "UniRef:UniRef100_UPI00158F3401" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158F3401", "uniref90_id": "UniRef90_D1J7T1" }, "psc": { "uniref90_id": "UniRef90_D1J7T1", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7T1" }, "pscc": { "uniref50_id": "UniRef50_D1J7T1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T1" ], "product": "Lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_344", "locus": "GCA013368035_00317" }, { "type": "cds", "sequence": "contig_1", "start": 177664, "stop": 178080, "strand": "+", "frame": 1, "gene": "rpsL", "product": "30S ribosomal protein S12", "db_xrefs": [ "COG:COG0048", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02950", "RefSeq:WP_012855422.1", "SO:0001217", "UniParc:UPI0001BC2102", "UniRef:UniRef100_D1J7T2", "UniRef:UniRef50_P92532", "UniRef:UniRef90_A0A858U5X2" ], "nt": "ATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCCGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTGCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATCGTTCGTGGAACACAAGATGCTGCTGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAA", "aa": "MPTVAQLVKQGRKDKTTKSKAPALGKMYNSLQKKETAMPAPFKRGVCTRVATMTPKKPNSAIRKYARVRLSNGQEVTAYIPGEGHNLQEHSVVLIRGGKVKDLPGVRYTIVRGTQDAAGVNNRKQARSIYGTKKPKSN", "aa_hexdigest": "f80b852c405717dd77647c74b99dac37", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2102", "ncbi_nrp_id": "WP_012855422.1", "uniref100_id": "UniRef100_D1J7T2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2102", "RefSeq:WP_012855422.1", "UniRef:UniRef100_D1J7T2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7T2", "uniref90_id": "UniRef90_A0A858U5X2" }, "psc": { "uniref90_id": "UniRef90_A0A858U5X2", "gene": "rpsL", "product": "30S ribosomal protein S12", "uniref50_id": "UniRef50_P92532", "cog_id": "COG0048", "cog_category": "J", "kegg_orthology_id": "K02950", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "genes": [ "rpsL" ], "id": "GLJLFKCOHC_345", "locus": "GCA013368035_00318" }, { "type": "cds", "sequence": "contig_1", "start": 178111, "stop": 178419, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "nt": "ATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAATGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGA", "aa": "MSRKHKAPVRDVLADPVFNSKIITKLVNTIMLDGKKSIAENILYSAFDIIKTKTGKEPVEVFNLALENVSPQLEVRSRRVGGSNYQVPCEVSAKRKQTLALR", "aa_hexdigest": "a846a895e05ab362d597ef3c44d7d680", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A381KMK1", "query_cov": 1.0, "subject_cov": 0.8429752066115702, "identity": 0.5589999999999999, "score": 124.0, "evalue": 1.23e-34, "valid": false, "gene": "rpsG", "product": "30S ribosomal protein S7", "uniref50_id": "UniRef50_A0A381KMK1", "kegg_orthology_id": "K02992", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_A0A381KMK1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A381KMK1" ], "product": "Ribosomal protein S7" }, "genes": [], "id": "GLJLFKCOHC_346", "locus": "GCA013368035_00319" }, { "type": "cds", "sequence": "contig_1", "start": 178456, "stop": 178578, "strand": "+", "frame": 1, "gene": null, "product": "Ribosomal protein S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A948V4F4", "UniRef:UniRef90_J9ZBC4" ], "nt": "ATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGA", "aa": "MEEKLANELIDASNKVGGAIKKREDTHKMAESNKAFAHFR", "aa_hexdigest": "c45ebf744fe7d637b0f9acfff3932c27", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A380FBG9", "query_cov": 1.0, "subject_cov": 0.8, "identity": 0.775, "score": 69.7, "evalue": 4.19e-15, "valid": false, "product": "30S ribosomalprotein S7p (S5e)", "uniref50_id": "UniRef50_A0A645CV26" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 178405, "stop": 178578, "observations": { "mutation": [ 178417 ], "stop": [ 178417 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_J9ZBC4", "query_cov": 1.0, "subject_cov": 0.6896551724137931, "identity": 0.8, "score": 69.3, "evalue": 7.18e-15, "gene_start": 1, "gene_end": 40, "reference_start": 18, "reference_end": 57, "reference_sequence": "MALRWIVDSAYKRAGHSMEEKLAGELLDASNNTGGAVKKREDTHRMAEANKAFAHYRW" }, "paralog": false, "identity": 0.6666666666666666, "score": 79.0, "evalue": 1.16e-21, "description": "Internal stop codon at 178417. Nonsense mutation around 178417.", "psc": { "uniref90_id": "UniRef90_J9ZBC4", "product": "Ribosomal protein S7", "uniref50_id": "UniRef50_A0A948V4F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_J9ZBC4", "UniRef:UniRef50_A0A948V4F4" ] } }, "id": "GLJLFKCOHC_347", "locus": "GCA013368035_00320" }, { "type": "cds", "sequence": "contig_1", "start": 178594, "stop": 178749, "strand": "+", "frame": 1, "gene": "lepA", "product": "GTP-binding protein lepA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4", "UniRef:UniRef90_A0A3P8KNQ4" ], "nt": "ATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATTATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGA", "aa": "MSREYKLEDYRNIGIMAHIDAGKTTTTERVLYHTGKIHKIGETHEGASQMD", "aa_hexdigest": "819aa66155ed42a00a7c7201fbcb4d6f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003516DBAA", "query_cov": 1.0, "subject_cov": 0.9622641509433962, "identity": 0.725, "score": 84.7, "evalue": 8.03e-21, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A962BSW7" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 178594, "stop": 178806, "observations": { "mutation": [ 178747 ], "stop": [ 178747 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "query_cov": 1.0, "subject_cov": 0.6891891891891891, "identity": 0.882, "score": 100.0, "evalue": 5.36e-27, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MARQYKLEDYRNIGIMAHIDAGKTTTTERILLHTGKIHKLGETHDGASQMDWMAQEQERGITITSAATNSILKW" }, "paralog": false, "identity": 0.8571428571428571, "score": 126.0, "evalue": 7.92e-40, "description": "Internal stop codon at 178747. Nonsense mutation around 178747.", "psc": { "uniref90_id": "UniRef90_A0A3P8KNQ4", "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A3P8KNQ4", "UniRef:UniRef50_A0A3P8KNQ4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3P8KNQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3P8KNQ4" ], "product": "GTP-binding protein lepA" } }, "id": "GLJLFKCOHC_348", "locus": "GCA013368035_00321" }, { "type": "cds", "sequence": "contig_1", "start": 179002, "stop": 180117, "strand": "+", "frame": 1, "gene": null, "product": "GTP dependent elongation factor G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "nt": "ATGGACAAGATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGTGCTAATGCTCACGCTATTCAATTAAATATTGGTGAAGAAAACCATTTTGTTGGAATCATTGATTTAGTTGAAATGAAAGCATATTCATATGATGGCGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGGGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGATATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAGGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGATGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGTAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTGAAAGTAACAACCACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCCGCTGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGTGCCCCACAAGTTTCATATCGTGAAACCATTACAAAGACTGCCGATGTTGAAGGTATGCACAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGA", "aa": "MDKMGANFKASVESLHKLLGANAHAIQLNIGEENHFVGIIDLVEMKAYSYDGEVDENAKEIAIPEHLKDEAHIMRAELAESLVDYDEELMTLLLEEKEVPVDILKRAIRTATISSEYFPVVCGTSFKNKGVKLMLDAIVDYLPSPLDIPPMKAYKGESEIHIPASDDEFFSALAFKVMNDPFVGNLTYFRVYSGIVSKGSYVFNSTKGEKERLSRILLMHANSRTDIDEVRTGDIAAAVGLKVTTTGDTLIDEKQKDVVLENMNFPEPVISQAIEPKTKDASEKLSLALQRLAAEDPTFKYYTDEETGQTIIAGMGELHLDIIVDRLRREFKVEVTVGAPQVSYRETITKTADVEGMHKKQSGGKGQYGHV", "aa_hexdigest": "f06d0300766152dd6315283aa9608f58", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000DB1047A", "query_cov": 0.9649595687331537, "subject_cov": 0.8306264501160093, "identity": 0.531, "score": 344.0, "evalue": 1.02e-112, "valid": false, "product": "translation factor GTPase family protein", "uniref50_id": "UniRef50_A0A346FYQ4", "kegg_orthology_id": "K02355" }, "pscc": { "uniref50_id": "UniRef50_A0A346FYQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A346FYQ4" ], "product": "GTP dependent elongation factor G" }, "genes": [], "id": "GLJLFKCOHC_349", "locus": "GCA013368035_00322" }, { "type": "cds", "sequence": "contig_1", "start": 180229, "stop": 180600, "strand": "+", "frame": 1, "gene": null, "product": "Elongation factor G domain protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "nt": "ATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGACAAGTTAGAGATAACGAAACAAGAAACGATGGAGCTCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGA", "aa": "MAIGILAGYPMIDIKATLFDGSYHEVDSSELAYKIAASKSLTKGREALGTVLLEPIMDVAVVVPEDFFGDVMGDISRRRGQVRDNETRNDGAHVIKAYIPLSEMFGYATQLRSMTTGRGTYQM", "aa_hexdigest": "96f465eb36f63e8aba1a1d4f44085104", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449AGV8", "query_cov": 1.0, "subject_cov": 0.9318181818181818, "identity": 0.772, "score": 194.0, "evalue": 3.83e-62, "valid": false, "product": "Elongation factor G domain protein", "uniref50_id": "UniRef50_A0A449AGV8" }, "pscc": { "uniref50_id": "UniRef50_A0A449AGV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AGV8" ], "product": "Elongation factor G domain protein" }, "genes": [], "id": "GLJLFKCOHC_350", "locus": "GCA013368035_00323" }, { "type": "cds", "sequence": "contig_1", "start": 180946, "stop": 181161, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCAAGAAACTCGAGATAAATTAATTTTAGCTAATGCCTTGCCTTCTGTTTCGGGTGCAATGTTACAAGTTAGATTTACATTTACCTATATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTACTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTGGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGA", "aa": "MSQETRDKLILANALPSVSGAMLQVRFTFTYISNILIGFALIFYGLLPNYLWTKRGFFLATVYINITFFTF", "aa_hexdigest": "53e70e2870cc45461ed2b56fb7457907", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8180.5798999999915, "isoelectric_point": 9.694836235046388 }, "id": "GLJLFKCOHC_351", "locus": "GCA013368035_00324" }, { "type": "cds", "sequence": "contig_1", "start": 181387, "stop": 181710, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGACTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGTTATCATGGCAATAATTTGTTTTATAAAATTCTATTAAATTTATTTATTCCTATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCGTTTTTAAGAGAAAATAAGCTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAA", "aa": "MQVTNLFLIEQFNKKTTGLDIFTKTKVAIYPFLNLKYPLGYHGNNLFYKILLNLFIPIFGIIVCISVAYFLKFICKIQTYSKKDFENPAFLRENKLLIKSIKSQIFK", "aa_hexdigest": "4d283377d9f4a9083156c97c15d29ca5", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.982599999981, "isoelectric_point": 9.758853340148924 }, "id": "GLJLFKCOHC_352", "locus": "GCA013368035_00325" }, { "type": "cds", "sequence": "contig_1", "start": 181722, "stop": 182351, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "ATGACGGCTTTTAATTTAAAAAAATATTTTGAGTTACAAACAATTGAAATTAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGTGGCAAAATTTTTGACGATTTACATGCCTCAAGGGTTTTACCCGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTGAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCTAATGATATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGACGATGTATTGAGACTAATTGATAACTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAACCAACATTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGTTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAATAATATAGATTCTGGTTATGCAAAATTTGAAACATTTCCCGTTTGA", "aa": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQPTFRVNKLQNILSEHNINVVKHYKIDDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPV", "aa_hexdigest": "7ec6c64c5c6133c238e47fec504b43df", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0025EA5487", "query_cov": 0.9904306220095693, "subject_cov": 0.8922413793103449, "identity": 0.57, "score": 244.0, "evalue": 7e-79, "valid": false, "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_A0A348P2W6" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 181722, "stop": 182654, "observations": { "mutation": [ 182349 ], "stop": [ 182349 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.4247967479674797, "identity": 0.99, "score": 408.0, "evalue": 7.63e-140, "gene_start": 1, "gene_end": 209, "reference_start": 1, "reference_end": 209, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.9903225806451613, "score": 610.0, "evalue": 1.06e-221, "description": "Internal stop codon at 182349. Nonsense mutation around 182349.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "GLJLFKCOHC_353", "locus": "GCA013368035_00326" }, { "type": "cds", "sequence": "contig_1", "start": 182373, "stop": 183200, "strand": "+", "frame": 3, "gene": null, "product": "DUF1846 domain-containing protein", "db_xrefs": [ "COG:COG4868", "COG:S", "SO:0001217", "UniRef:UniRef50_D1J7T6", "UniRef:UniRef90_D1J7T6" ], "nt": "TTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATCAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGTGATATTCAAGCATTTCCAATTTTAAGTCAAATAATGAACAAAATTCAAAAGCGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGATGAAATTAAACAAGCGGCAAATAAAGAAATAATTCGTCGCTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTGATTTTTCAATTAGACCTTGCATTGAAATTGCTAACAAATTAGCAAATCAATTGAATATGAATATCACAACTTTTACCTTAAACAACAATATAAATATTACAGGTAAGACATCAATGCTCCTTGGTTCATCATCAGCTGCGTTATTAAATGCATTAAAATATTTAGCAAATATTCCTAAAGAAATCCATTTACTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGTAAAAATCCCCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAATCAATATGCAAAATTAGCCTATGATCAACTAAAAAATTTAGCAGGTATCGAAGCACATTGCAGCGCAATACTTTCTATGCAAGATATTGAAACATACAATCACTTAAATATTAACATTACTCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAG", "aa": "MNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINITGKTSMLLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK", "aa_hexdigest": "47e1b211b660970368dcd70393ee1e85", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 182073, "stop": 183200, "observations": { "mutation": [ 182349 ], "stop": [ 182349 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7T6", "query_cov": 1.0, "subject_cov": 0.5589430894308943, "identity": 0.9890000000000001, "score": 523.0, "evalue": 6.22e-184, "gene_start": 1, "gene_end": 275, "reference_start": 218, "reference_end": 492, "reference_sequence": "MTAFNLKKYFELQTIEIKNRIKQFNNKLYLEFGGKIFDDLHASRVLPGFLSDSKLQLLLKLKNEAEIVVVISANDIENNKLRGDLGIKYEDDVLRLIDNFKSYDLIVNNVVITQYQSTFRVNKLQNILSEHNINVVKHYKIEDYPNNLDLIVSKNGYGKNETIKTQKPLIVITAPGPGSGKLAVALSELYHDAINNIDSGYAKFETFPVWNLAVDHPLNLAYEAATTDLNDQNKIDPYYLKSRNKIATNYNRDIQAFPILSQIMNKIQKRNIYDSPTSMGVNMVGFCFNNEDEIKQAANKEIIRRYYQSAVAFKKGMVSKSVLIRNANLISKNNIDFSIRPCIEIANKLANQLNMNITTFTLNNNINIIGKTSALLGSSSAALLNALKYLANIPKEIHLLSKEAIELVQNLKVNVFKSKNPRLHMNETLIILAMSAKSNQYAKLAYDQLKNLAGIEAHCSAILSMQDIETYNHLNINITQEPKYDNDTLYHK" }, "paralog": false, "identity": 0.9893333333333333, "score": 733.0, "evalue": 5.94e-269, "description": "Internal stop codon at 182349. Nonsense mutation around 182349.", "psc": { "uniref90_id": "UniRef90_D1J7T6", "product": "DUF1846 domain-containing protein", "uniref50_id": "UniRef50_D1J7T6", "cog_id": "COG4868", "cog_category": "S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7T6", "UniRef:UniRef50_D1J7T6", "COG:COG4868", "COG:S" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7T6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7T6" ], "product": "DUF1846 domain-containing protein" } }, "id": "GLJLFKCOHC_354", "locus": "GCA013368035_00327" }, { "type": "cds", "sequence": "contig_1", "start": 183569, "stop": 183730, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00383.29" ], "nt": "ATGTCACTTGCCAAATTAAGTGCATTACGTTCGAAAGATCCTTTAACTAAAGTAGGAGCTTGTATTGTAAGTCCAGATAATTATGTAATATCGCTAGGATATAATGGTATGCCAACAAGTTATTTAAATAAAGAAGTTAACAATGATGATTTATTCACTTGA", "aa": "MSLAKLSALRSKDPLTKVGACIVSPDNYVISLGYNGMPTSYLNKEVNNDDLFT", "aa_hexdigest": "ffd5a4a11e098e12cfaf5cd3ed4e2701", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00383.29", "name": "dCMP_cyt_deam_1", "length": 42, "aa_cov": 0.7735849056603774, "hmm_cov": 1.0, "evalue": 4.187373028621015e-07, "score": 25.266199111938477, "start": 1, "stop": 41 } ], "seq_stats": { "molecular_weight": 5765.546200000001, "isoelectric_point": 5.952713584899903 }, "id": "GLJLFKCOHC_355", "locus": "GCA013368035_00328" }, { "type": "cds", "sequence": "contig_1", "start": 184978, "stop": 185151, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCTTTGCCGGTAACATCTGTTACAGTTTTAGTTGGTTGTGGATATTCAGATTCACTTGGAATTTCATCTGAATCAAATACATATTGTTTATTAGTTCCTTTTTCAGTTAAAACAATAGCTTCTGCTAATTCTAATCTATATTTAGCAAAAGTAGGTTTTACTAAATAG", "aa": "MYSLPVTSVTVLVGCGYSDSLGISSESNTYCLLVPFSVKTIASANSNLYLAKVGFTK", "aa_hexdigest": "55ad1ec1639ab05a431579da9c12cbe5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5997.860800000002, "isoelectric_point": 7.759852409362794 }, "id": "GLJLFKCOHC_356", "locus": "GCA013368035_00329" }, { "type": "cds", "sequence": "contig_1", "start": 185504, "stop": 185848, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGTAATTTAAAAGATAGAGAAGCAATTACTTTTGAATCAATTACTGGAAAAGAAAAAGATGCCAACATTTCAAGCTTCTTTGAAAGTCAATTTGCATCAGGAACATATGACTTTATGCCTGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAACGGTATTGCTTTATCAAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGATAGCTTACAATACAAAATTGGTCAATACTGA", "aa": "MFGIDANKLFEKDKFIQKVSAEGNLKDREAITFESITGKEKDANISSFFESQFASGTYDFMPAPSDYIDALNAKNEQVVLKSNGIALSKSETEAYRNALNSMDKDSLQYKIGQY", "aa_hexdigest": "def054b0d21fa470d31407926cf7081c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12769.073399999985, "isoelectric_point": 4.825994300842285 }, "id": "GLJLFKCOHC_357", "locus": "GCA013368035_00330" }, { "type": "cds", "sequence": "contig_1", "start": 186143, "stop": 186373, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTAAATGAACGTTTGAATGTCCAAACTTCAATCTTTGGATTTGTTAGAACACCATTTATTTCTTCAAAATTAGACAAAAATTACAAATTCAATGAAAACTTTGCTAAATTAGTATACGGATCTTCATTAGCTGATATTGACAGTGGAAAAGCAGACACTTCAAAATCATACCTATTTGGTAAAGGTTTAAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGA", "aa": "MKLNERLNVQTSIFGFVRTPFISSKLDKNYKFNENFAKLVYGSSLADIDSGKADTSKSYLFGKGLSFRTLLYAAVN", "aa_hexdigest": "d32bddd9264427f3f5464a36590d291a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8537.666299999997, "isoelectric_point": 9.696899223327637 }, "id": "GLJLFKCOHC_358", "locus": "GCA013368035_00331" }, { "type": "cds", "sequence": "contig_1", "start": 186476, "stop": 186790, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACACTATTACAGACTTTAGAGACCAAGCTAACTCACTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTCGGTGCTGAATTAGGTAAGGAATTAAGTCCTTCTGAAAACACCAAAGCTTCAGAAACTGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTAAAGAAAAAATGAAAGAATTACTAGATCAATTCTTTAAAGAACAAAACATTACCGATCCTGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATTAATGCATGA", "aa": "MNTITDFRDQANSLFAIDKDGNKVDFGAELGKELSPSENTKASETESSISEKMKSAGFKVLKEKMKELLDQFFKEQNITDPEKQTIEFEVGFRYLNAPQLYINA", "aa_hexdigest": "cd3b07c72e57d14c33dc6983d0362d8a", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11819.111699999989, "isoelectric_point": 4.776658058166503 }, "id": "GLJLFKCOHC_359", "locus": "GCA013368035_00332" }, { "type": "cds", "sequence": "contig_1", "start": 186995, "stop": 187333, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAAAGGTTGCACACCAAACAGGCGCTGAAAAAGCTAAATTTGAAAAAGCCTTCCCAGAAATTGCTAAATTAGCTGATAAATTGTTAAAATATGCAGAAACAAACAAACCAAATTTATCATTGGATTTTGATAAAATTGATCAAATTGACAATAGATATTTAAATTCAAACTTACCTTCAATTTTAGCATCATATAAATTAGAACAAGATACCAATACTAAAAAATATGTAATTGCTAAAAAAACTGTTAAAGGTTCAAAACCAAAACCAATCGAATTCGATTTTGGAAAAGACAGTTCAGGAAAATCAAAAGAACCAACCGATCTATATCAATGA", "aa": "MVKVAHQTGAEKAKFEKAFPEIAKLADKLLKYAETNKPNLSLDFDKIDQIDNRYLNSNLPSILASYKLEQDTNTKKYVIAKKTVKGSKPKPIEFDFGKDSSGKSKEPTDLYQ", "aa_hexdigest": "bf3dde0b219851d751e0743db9cd8135", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12655.319199999985, "isoelectric_point": 9.280368995666507 }, "id": "GLJLFKCOHC_360", "locus": "GCA013368035_00333" }, { "type": "cds", "sequence": "contig_1", "start": 187439, "stop": 187537, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTGTTGGCCGTGATAAATTTGTTAAAACTTTAATAAACAAACACTACGTTTCACCAACTATTGCAGGATCAGCAGTTGATTCATACCAAGATTGA", "aa": "MFVGRDKFVKTLINKHYVSPTIAGSAVDSYQD", "aa_hexdigest": "0f1c52d4be80ccbd4b910d236cc6f9e8", "start_type": "GTG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3588.0513, "isoelectric_point": 8.161133384704591 }, "id": "GLJLFKCOHC_361", "locus": "GCA013368035_00334" }, { "type": "cds", "sequence": "contig_1", "start": 187580, "stop": 188038, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAATATATTTTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTAGTTGCTGCATTTGGAAACAATCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATGCTGAAATATTGCATAAACTTGAAATTGAAAACGGATTAAGATATAAAGATAACCCGGACATTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAACAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATACTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCTGGATATAAGCGTGGAAAATGA", "aa": "MTKYILQRIAFAILTLFIISIFVYVLVAAFGNNPVNDLAQAQFQASKKSGLTYAEILHKLEIENGLRYKDNPDILVPIIVRYGNYMKNIFTKGDFGFLYNPANNPNSALYTNMAKLFFTPLQYSLIITVPTFFISSILGIAIGVIAGYKRGK", "aa_hexdigest": "f3d9d05c9d6500ba6652e02851e1b863", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17056.863199999985, "isoelectric_point": 9.63056125640869 }, "id": "GLJLFKCOHC_362", "locus": "GCA013368035_00335" }, { "type": "cds", "sequence": "contig_1", "start": 188045, "stop": 188434, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTCTTCAAATGTTTTTGTGTTAATATTTGTTGCTATACCATCATTTATATTGGCTCCTATTGTAATAACTATTTCAGTCGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTTGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGCTATGTAACATACACAAGAAATCAAGTTATTACAGTATTAACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCTTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGA", "aa": "MSSNVFVLIFVAIPSFILAPIVITISVALGISSVVPRPGEQSFGVMFVSYLPPIFVMSITSLAGYVTYTRNQVITVLTSNFVLIAKTKGLSSSQIFFKYVLRNISIPLFSLVFGSFIGLLSGSIIIEQY", "aa_hexdigest": "c44196720190f8b1fd87aec40f105bd6", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14021.567999999983, "isoelectric_point": 9.77445468902588 }, "id": "GLJLFKCOHC_363", "locus": "GCA013368035_00336" }, { "type": "cds", "sequence": "contig_1", "start": 188257, "stop": 188604, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTAGTAGAATATTTAATCTTAGGATCTAATATTGCATATGAAACGTCAATAATAATATCGGCAATTAGCGATATAAATGTAAAGAATAATGTAGAGAACATAACAACATTAATTTCTCCGGTAGGGAAGGCATTAACTATTATTTGTGATGTTCCTGGTACTTGTCAATATTGTTCAATAATTATTGATCCTGATAATAGACCAATAAATGACCCAAAAACTAAGCTAAATAAAGGAATTGAAATATTTCTTAAAACATATTTAAAGAAAATTTGTGAAGAACTCAATCCTTTTGTTTTAGCTATTAAAACGAAATTTGATGTTAATACTGTAATAACTTGA", "aa": "MLLVEYLILGSNIAYETSIIISAISDINVKNNVENITTLISPVGKALTIICDVPGTCQYCSIIIDPDNRPINDPKTKLNKGIEIFLKTYLKKICEELNPFVLAIKTKFDVNTVIT", "aa_hexdigest": "420c54cec306c5dbefb6f0715a2499a0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12757.913799999977, "isoelectric_point": 5.326404762268066 }, "id": "GLJLFKCOHC_364", "locus": "GCA013368035_00337" }, { "type": "cds", "sequence": "contig_1", "start": 188727, "stop": 189353, "strand": "+", "frame": 3, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7U0", "UniRef:UniRef90_D1J7U0" ], "nt": "ATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCACCAGAAGATAGAATAGTAATTTCTTCTGTTGCGGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCTCCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAGGTTAATGATATCGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATATTTAAAAATATTTAATGAACAAAAAAACTTTTATTCTCATGTTTTATTAGGAACATCATCAATAGGCTACGATATTTGA", "aa": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDIGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDI", "aa_hexdigest": "6c1ffa72144f196a76b4ddf538e30e54", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 188727, "stop": 189656, "observations": { "mutation": [ 189351, 189363, 189375, 189528, 189600 ], "stop": [ 189351, 189363, 189375, 189528, 189600 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U0", "query_cov": 1.0, "subject_cov": 0.49056603773584906, "identity": 1.0, "score": 396.0, "evalue": 3.99e-136, "gene_start": 1, "gene_end": 208, "reference_start": 1, "reference_end": 208, "reference_sequence": "MNINEVKAFNKEYKINTELEKKFKFVAPEDRIVISSVAGKPKKLGIEILKRFFTKPVVLIALAVFIIILLLSILVPANGISYYKPNDPISDNAFVKNLPPASSPIVTQALESNDPVFKFYQFVLNRSETDPVYKARMAFFLNGFQAKVNDIGIYVAKYNAYDLVVANYINGIISKNQITDAEYLKIFNEQKNFYSHVLLGTSSIGYDIWTTAWYATWRAIKIALIVVILEAIIGISIGAFLGFYAGKLIDTIIMRVIEIFLAPPTLIWILLFVSIIGANEWSLVLTLTIVGWPGFVGITRMFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVILWIASLAFLGFFKEKKDTNLGQMLIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK" }, "paralog": false, "identity": 0.8867313915857605, "score": 514.0, "evalue": 8.99e-185, "description": "Internal stop codon at 189351, 189363, 189375, 189528, 189600. Nonsense mutation around 189351, 189363, 189375, 189528, 189600.", "psc": { "uniref90_id": "UniRef90_D1J7U0", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7U0", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U0", "UniRef:UniRef50_D1J7U0", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U0" ], "product": "Oligopeptide transport system permease protein" } }, "id": "GLJLFKCOHC_365", "locus": "GCA013368035_00338" }, { "type": "cds", "sequence": "contig_1", "start": 189627, "stop": 189782, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCATTATTACAGTAAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTCGTTCATGCATTACCAACTATAATTGGAAAAATTGCAAATAATATAGTAAGATCAGTACCAGGCGTTATTCTATGA", "aa": "MFIITVKDEEFIIAAKAIGVGTPRRIFVHALPTIIGKIANNIVRSVPGVIL", "aa_hexdigest": "766e71a14c489f6cc7701055b90c904a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5513.6307, "isoelectric_point": 10.266412925720214 }, "id": "GLJLFKCOHC_366", "locus": "GCA013368035_00339" }, { "type": "cds", "sequence": "contig_1", "start": 189846, "stop": 190001, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGAAGCTTCGCAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATTGCTCTAGGTGTGCATGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAA", "aa": "MIEASQEVGSNIWIIALPTIILLFLSLSLNFIALGVHDALDPRVMNKGKGK", "aa_hexdigest": "7a6ce3add55c99c33ec77b3057c90848", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5548.563800000003, "isoelectric_point": 6.518659400939941 }, "id": "GLJLFKCOHC_367", "locus": "GCA013368035_00340" }, { "type": "cds", "sequence": "contig_1", "start": 190003, "stop": 190377, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGGCAAAAATTATTAATAAACACGACAATTTAGACAAAAACAACTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATTTGCATGTTAGTTTCCCTCAAGGTCATAAGAAAAGTATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGTGAAATAATTGGTCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACCTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGA", "aa": "MAKIINKHDNLDKNNSDTIKHQKINKSTRVLDSIRVEPLKNNQILKVRNLHVSFPQGHKKSIHIVRGVDLDVHRGEIIGLVGESGSGKSVTSKTLINVNESGIISADQIQIGEYELTNIKKQKY", "aa_hexdigest": "978d8cfe3751dc08c2b82ae026a3035f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 56, "aa_cov": 0.43548387096774194, "hmm_cov": 1.0, "evalue": 1.10427340010276e-09, "score": 34.42229080200195, "start": 66, "stop": 119 } ], "seq_stats": { "molecular_weight": 13886.74759999999, "isoelectric_point": 9.734097480773926 }, "id": "GLJLFKCOHC_368", "locus": "GCA013368035_00341" }, { "type": "cds", "sequence": "contig_1", "start": 190555, "stop": 190890, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "nt": "TTGCGTTCGGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCAGGGGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATCGCTGATGAACCAACAACGGCATTAGACCCAACAGTTCAAGCCAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTACATTTATGTTATGTATGCTGGTAGAATTGTTGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGA", "aa": "MRSAEKIFYAYPHTLSGGMKQRIVITMVVALKPELIIADEPTTALDPTVQASVLALFEDIRQKMGISIILISHNISVIAKFCDYIYVMYAGRIVEKGTKQEIFTNPAHPYT", "aa_hexdigest": "aa241ded370b8c4f868d7d0398dcf46a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000FD1CF63", "query_cov": 0.9099099099099099, "subject_cov": 0.9439252336448598, "identity": 0.525, "score": 119.0, "evalue": 7.69e-33, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000FD1CF63" }, "pscc": { "uniref50_id": "UniRef50_UPI000FD1CF63", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000FD1CF63" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_369", "locus": "GCA013368035_00342" }, { "type": "cds", "sequence": "contig_1", "start": 190960, "stop": 191079, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTATGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGA", "aa": "MANLPLGDPFVYRNDYALEIDYLKEPPLIPITETHAAAT", "aa_hexdigest": "89750929c15eade9b04e273a06f3c46d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4373.931700000001, "isoelectric_point": 4.290286827087402 }, "id": "GLJLFKCOHC_370", "locus": "GCA013368035_00343" }, { "type": "cds", "sequence": "contig_1", "start": 191162, "stop": 191578, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "nt": "ATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATTTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACCGTTGGTTCAACAATACTTAGACTATATGATGATTACAACGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGCATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCTCATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAAGATATTTTTAAAGATTGA", "aa": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKD", "aa_hexdigest": "91715e0a19db33f76a755f8881eb8d8a", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI00249F2F44", "query_cov": 0.9202898550724637, "subject_cov": 0.9921875, "identity": 0.701, "score": 184.0, "evalue": 1.13e-57, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI000CA0BF5C" }, "pscc": { "uniref50_id": "UniRef50_UPI000CA0BF5C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000CA0BF5C" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_371", "locus": "GCA013368035_00344" }, { "type": "cds", "sequence": "contig_1", "start": 191807, "stop": 192976, "strand": "+", "frame": 2, "gene": "dppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "db_xrefs": [ "COG:COG0444", "COG:EP", "SO:0001217", "UniRef:UniRef50_B3PML2", "UniRef:UniRef90_D1J7U2" ], "nt": "ATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGACCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTCAATGTTTAAGCAAAACTTCAAAGATTGCTTATGAAGCTTCGCTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAAAATACATTTGCAAACTATATTCATGAATCGAAAAATGAAAAGAGTTTAATTGACATAGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAATAAAAGGGGTCAAGTATTGGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGACATTAAAAATGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCAAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGTAGATCTAAATATTTAATTTCATATAAAGCTGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTGAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGA", "aa": "MYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFDEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKDKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHK", "aa_hexdigest": "8e467af0c96eb13097d60070f53b80ed", "start_type": "ATG", "rbs_motif": null, "genes": [ "dppD" ], "truncated": "both", "pseudogene": { "start": 191507, "stop": 193279, "observations": { "mutation": [ 191576, 191687, 192974, 193043 ], "stop": [ 191576, 191687, 192974, 193043 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U2", "query_cov": 1.012853470437018, "subject_cov": 0.4651711924439197, "identity": 0.982, "score": 712.0, "evalue": 4.18e-251, "gene_start": 1, "gene_end": 389, "reference_start": 216, "reference_end": 609, "reference_sequence": "MNNKNFKDKKVILSIDNLKKYFVNQGMINKAVDGVSFDVHEGEIVGLIGESGSGKTTVGSTILRLYDDYNGFVRLEDKIISGKRISESLNRFLRKNVQMIFQDPHASLNSQQNIYSILKEPLLVNGVMKDKIKDIFKDWLNVKKNFKYTFQIEAMRLELQNYLEINKLAEPLFKKWTENLNKLEFNFDISREDNFSLFFGYLEEKQNVESIIINNMYANVDRLIEFYYETQRKYRNKELPGPQIALNEKEEKLKYIQCLSKTSKIAYEASLELKEVKKQIFNLRHKIEKISSESKNTFANYIHESKNEKSLIDIARLMSCDLDFYSYNLKSELLMNKRGQVLAKIKPFNKFLGFNNIKKLINELDEYINEFYKTKLAPLPYSKTLKSDIKNVLEKDFDFKFNEYTKISELEKQKLDSELKQLLSREVEIKKVIKENPNPEITSEQLQEAKNDLELGKQAYLEGRSKYLISYKAELESLYKNLEEQKKFYLELRKQQDYCNNKYKELKQKFFEYVEQLRQKEKDKILLKIAELKQNQDKSNKANNINLKKSLAKLQSKLKSIKVQYNTILKMYHSDISLKEDTLKSFDIERKYLDKDINNIYVLLGIDHKWVETNLKTADAMAGVDAKHMKWVWRKRYFDSKVSYPIAKLLISSILYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQWTHNAKLEKEHKEL" }, "paralog": false, "identity": 0.9815126050420168, "score": 1128.0, "evalue": 0.0, "description": "Internal stop codon at 191576, 191687, 192974, 193043. Nonsense mutation around 191576, 191687, 192974, 193043.", "psc": { "uniref90_id": "UniRef90_D1J7U2", "gene": "DppD", "product": "Oligopeptide transport ATP-binding proteinhomolog", "uniref50_id": "UniRef50_B3PML2", "cog_id": "COG0444", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U2", "UniRef:UniRef50_B3PML2", "COG:COG0444", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_B3PML2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PML2" ], "product": "Oligopeptide ABC transporter ATP-binding protein" } }, "id": "GLJLFKCOHC_372", "locus": "GCA013368035_00345" }, { "type": "cds", "sequence": "contig_1", "start": 193109, "stop": 193648, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "nt": "TTGTATAAAACAATAATTTATAAAGCATTGGAAGATGTTGGACTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCGCAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTTGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTTTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGTGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGA", "aa": "MYKTIIYKALEDVGLLKQFAYRYPHEFSGGQLQRIVIARALIVEPQVIVADEPIASLDISIQAQVVNLLKELCIKKNIGLIFIAHDLSMIEYVADEVQIMHLGKIVESGKTEAIYANPIHPYTINLFKAIPKISNANEKFQNVSFALDYLDEQKFPNVPETFKVGEDHYVYGTASQLKQ", "aa_hexdigest": "f1efd8141ea362f19edc8aad725ebec4", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0024CC3F60", "query_cov": 1.0, "subject_cov": 0.8647342995169082, "identity": 0.737, "score": 267.0, "evalue": 7.16e-89, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A4R0XTG3" }, "pscc": { "uniref50_id": "UniRef50_A0A4R0XTG3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A4R0XTG3" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_373", "locus": "GCA013368035_00346" }, { "type": "cds", "sequence": "contig_1", "start": 193759, "stop": 194481, "strand": "-", "frame": 2, "gene": "rsuA", "product": "Pseudouridine synthase", "db_xrefs": [ "COG:COG1187", "COG:J", "RefSeq:WP_012855433.1", "SO:0001217", "UniParc:UPI0001BC2137", "UniRef:UniRef100_D1J7U3", "UniRef:UniRef50_D1J7U3", "UniRef:UniRef90_D1J7U3" ], "nt": "ATGGAAGAAAAAGCAAAACGAATTCAAAAAATTTTAAGTGAGCAAGGCATTTGTTCTAGAAGAAAAGCAGAAGAATTAATTTTATCGGGGAAGATTTTAATAAATAATAAAATTGCAAAATTAGGCGAATGTGTAACTATAAATGACGAAATTAAAATAAACAATAGAATTATCAAATTAAATAATAATATTGCAAATAATTTTAAATACATTGTTTTAAATAAACCCATGAATACAATTTGTACAGCAAGTGATCCTCAAAATAGAAAAACAATTTACGAATATCTCAAAGCAAAAGAACGCTTATTTTCAATAGGAAGATTAGACTTTAATACAACAGGAATTATATTAATCACAAACGATGGAGAATTTGCTAATTTATTAAGCCATCCTTCTTCTGAAATTCCTAGAATTTATATTGCTACATTAGAACGTGAAATGCTTGATGGTGAACTAAACTTATTAAATTCAAATCACGTATTATTAAATGGCAAAATTTCAAAACAAAAAGTTACAAAATTAAGTGCTTTTAAATATGAAGTTCTACTTTATGAAGGCAGAAACCATCACGTTAAAAATTTATTTTTGTTAGTAAATAATTATGTTAAAAAATTACATAGAAAATCTTTTGGATGCATTGATGATAGTAATTTAAAAATAGGTCAATATAGAAATTTAACTTCAAGCGAAATTAATAAATTATTAGCTTATATAAAGAAATAA", "aa": "MEEKAKRIQKILSEQGICSRRKAEELILSGKILINNKIAKLGECVTINDEIKINNRIIKLNNNIANNFKYIVLNKPMNTICTASDPQNRKTIYEYLKAKERLFSIGRLDFNTTGIILITNDGEFANLLSHPSSEIPRIYIATLEREMLDGELNLLNSNHVLLNGKISKQKVTKLSAFKYEVLLYEGRNHHVKNLFLLVNNYVKKLHRKSFGCIDDSNLKIGQYRNLTSSEINKLLAYIKK", "aa_hexdigest": "7f42f3834517523d9568b95d48a7b7ba", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2137", "ncbi_nrp_id": "WP_012855433.1", "uniref100_id": "UniRef100_D1J7U3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2137", "RefSeq:WP_012855433.1", "UniRef:UniRef100_D1J7U3" ] }, "ips": { "uniref100_id": "UniRef100_D1J7U3", "uniref90_id": "UniRef90_D1J7U3" }, "psc": { "uniref90_id": "UniRef90_D1J7U3", "gene": "RsuA", "product": "Pseudouridine synthase", "uniref50_id": "UniRef50_D1J7U3", "cog_id": "COG1187", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J7U3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U3" ], "product": "Pseudouridine synthase" }, "genes": [ "rsuA" ], "id": "GLJLFKCOHC_374", "locus": "GCA013368035_00347" }, { "type": "cds", "sequence": "contig_1", "start": 194589, "stop": 194843, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4", "UniRef:UniRef90_D1J7U4" ], "nt": "ATGGAATATTTACAAAATATAGACTTAAGCAAAATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGTTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGA", "aa": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESE", "aa_hexdigest": "cc38cd88563039e12c5649d4cb46963b", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 194589, "stop": 194927, "observations": { "mutation": [ 194841, 194856 ], "stop": [ 194841, 194856 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U4", "query_cov": 1.0, "subject_cov": 0.75, "identity": 1.0, "score": 162.0, "evalue": 1.84e-50, "gene_start": 1, "gene_end": 84, "reference_start": 1, "reference_end": 84, "reference_sequence": "MEYLQNIDLSKIRSLCLQKKREFIFNYCLFEQLKDKQLKQKKSQTINDEEDDDISKLEKFIKLLNSDNQKIFFNNFIYNNPESEWYLAYWSKNAYYKKLNYIVNLFIDFINQ" }, "paralog": false, "identity": 0.9821428571428571, "score": 221.0, "evalue": 3.4e-76, "description": "Internal stop codon at 194841, 194856. Nonsense mutation around 194841, 194856.", "psc": { "uniref90_id": "UniRef90_D1J7U4", "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U4", "UniRef:UniRef50_D1J7U4" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10245.543599999992, "isoelectric_point": 5.506072807312011 }, "id": "GLJLFKCOHC_375", "locus": "GCA013368035_00348" }, { "type": "cds", "sequence": "contig_1", "start": 195107, "stop": 195409, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGATAAACATGAATATACATATACTCTTATTTCTGAATTTAATGAGGCAACAAAAACTAAAAAAGTTCAGGATATAAAAAATATTAATTTTGAAATTTTTTATAAAGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAAATATATAGTAATAACAATTCTAATAAAGAATTAGTTTCAAATAAAAATGGCGTTGAATATGATTTATGA", "aa": "MNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNKELVSNKNGVEYDL", "aa_hexdigest": "50a6ee77e4bdafc253b266735c493145", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11951.205599999992, "isoelectric_point": 4.950130653381346 }, "id": "GLJLFKCOHC_376", "locus": "GCA013368035_00349" }, { "type": "cds", "sequence": "contig_1", "start": 195458, "stop": 195991, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8", "UniRef:UniRef90_A0A454C9K8" ], "nt": "ATGGAACATTATTCATTTGTTGAACAAGTTAAATACAAATTTATGCCAACTACTCTGGTGTTGGGAAAACATAATAAAAAATTATTTGATGTTGAAATATCAAAAAAAGAAATTTCTAACAAAAGTGGCATAATAAGCGATTTTTCTCAACTAATTCAAAGCGATATTAATGGTTTTAATGTATCTATTGATATTGATAAACAATTTATAAAAGGCAACTTGAATGCAAAAATTAATGATTTCAGTAAAAATAAGCATAAGCAAATTTCAATAGATTCTTATTCATATTATGACAAGGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGCGCAGCTAAAGGCTTATTGTTGCCTCTTAATTATTCAGGAAATTATTCTTATTTACTAAATATTAATTTTGGTGAGGATTTGAAAGGTTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAGTAAAATTAAATGTAAATAATATATTTAAACAACAAAATTTCATAAAATGA", "aa": "MEHYSFVEQVKYKFMPTTLVLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFSKNKHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIK", "aa_hexdigest": "651fc1c1705b8a9136ebaa0b7fd89a20", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 195158, "stop": 196072, "observations": { "mutation": [ 195407, 195989 ], "stop": [ 195407, 195989 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9K8", "query_cov": 1.0, "subject_cov": 0.4645669291338583, "identity": 0.9940000000000001, "score": 340.0, "evalue": 4.04e-115, "gene_start": 1, "gene_end": 177, "reference_start": 178, "reference_end": 354, "reference_sequence": "MFPIVMQAINDQEPTKWIENINAYTTKQLYLDKWTNSFQKVDFKLQRLIENNKFIIEFKYLNYDQEIDDIDVKCSINNQLLKLQKVFDSDKHEYTYTLISEFNEATKTKKVQDIKNINFEIFYKVKGLLYQVERYTYEIYSNNNSNRELVSNKNGVEYDLWTKLRILSLADRDIPTDMEHYSFVEQVKYKFMPTTLVLGKHNKKLFDVEISKKEISNKSGIISDFSQLIQSDINGFNVSIDIDKQFIKGNLNAKINDFSKNEHKQISIDSYSYYDKVSKAIIANNDSIGAAKGLLLPLNYSGNYSYLLNINFGEDLKGFKIRYNQEISKPFFNEKSGLVKLNVNNIFKQQNFIKWSVVKAEDIRSLINNSNTLEELMKGGK" }, "paralog": false, "identity": 0.9868421052631579, "score": 591.0, "evalue": 2.21e-216, "description": "Internal stop codon at 195407, 195989. Nonsense mutation around 195407, 195989.", "psc": { "uniref90_id": "UniRef90_A0A454C9K8", "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9K8", "UniRef:UniRef50_A0A454C9K8" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9K8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20294.975599999998, "isoelectric_point": 9.430322456359864 }, "id": "GLJLFKCOHC_377", "locus": "GCA013368035_00350" }, { "type": "cds", "sequence": "contig_1", "start": 196072, "stop": 196422, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6", "UniRef:UniRef90_D1J7U6" ], "nt": "ATGTTGATTGCAAAAAAGAAAAAAATAATTTTCGGTTTATCTCTTGGATTAGCTTGTGCCGTTGGCGCTGGCGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACAATAGTGACAACAAAAACATTAATTCTGGCAATGAAAACGAAAAGGATAACACTATTGATGGCCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGCCAAGCGAATGATGATGATAAAAAGCTTAAGCCAGAAGCAAAGCCAAGGCCGAATATAAAAACAGAGATTAATTGA", "aa": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNNSDNKNINSGNENEKDNTIDGQNEDSKIEDSNEDGQANDDDKKLKPEAKPRPNIKTEIN", "aa_hexdigest": "e7bb95217d8e3efddd828eb292922fb2", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 196072, "stop": 196677, "observations": { "mutation": [ 196420, 196618 ], "stop": [ 196420, 196618 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7U6", "query_cov": 1.0, "subject_cov": 0.5771144278606966, "identity": 0.905, "score": 146.0, "evalue": 2.5e-42, "gene_start": 1, "gene_end": 116, "reference_start": 1, "reference_end": 116, "reference_sequence": "MLIAKKKKIIFGLSLGLACAVGAGALVGVYFLASSNSNKKANNLDNKKVNKKDSTNNNSDNKNINSGNENEKDNTIDNQNEDSKIEDSNEDGQANDDDKKLKPEVKPRPNIKTEINWREIFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGDVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.9253731343283582, "score": 332.0, "evalue": 4.04e-118, "description": "Internal stop codon at 196420, 196618. Nonsense mutation around 196420, 196618.", "psc": { "uniref90_id": "UniRef90_D1J7U6", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7U6", "UniRef:UniRef50_D1J7U6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7U6" ], "product": "Lipoprotein" } }, "id": "GLJLFKCOHC_378", "locus": "GCA013368035_00351" }, { "type": "cds", "sequence": "contig_1", "start": 196507, "stop": 196620, "strand": "+", "frame": 1, "gene": null, "product": "MHO_1590 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0", "UniRef:UniRef90_UPI0035A6F3B0" ], "nt": "ATGATTACTTATATAATAAAGGATATTTTAAATAGGATGACTATTTTAGATGGCAATGTTTCTTATGCTTATAAACAAGAGAATGATCAAAATTTATATATTGCTTTTAAATGA", "aa": "MITYIIKDILNRMTILDGNVSYAYKQENDQNLYIAFK", "aa_hexdigest": "60fa67154dd3817b4c8ac3fe9e3b80f2", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 196429, "stop": 196677, "observations": { "mutation": [ 196618 ], "stop": [ 196618 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "query_cov": 1.0, "subject_cov": 0.45121951219512196, "identity": 0.973, "score": 75.9, "evalue": 2.96e-17, "gene_start": 1, "gene_end": 37, "reference_start": 27, "reference_end": 63, "reference_sequence": "MFPDVKTSDYYNQLNFKDGQGFIDKEMITYIIKDILNRMTILDGNVSYAYKQEDDQNLYIAFKWENKSQKSNITYKFSVNRI" }, "paralog": false, "identity": 0.9634146341463414, "score": 162.0, "evalue": 8.49e-54, "description": "Internal stop codon at 196618. Nonsense mutation around 196618.", "psc": { "uniref90_id": "UniRef90_UPI0035A6F3B0", "product": "MHO_1590 family protein", "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0035A6F3B0", "UniRef:UniRef50_UPI0035A6F3B0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0035A6F3B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035A6F3B0" ], "product": "MHO_1590 family protein" } }, "id": "GLJLFKCOHC_379", "locus": "GCA013368035_00352" }, { "type": "cds", "sequence": "contig_1", "start": 196686, "stop": 196910, "strand": "+", "frame": 3, "gene": "acpP", "product": "Acyl carrier protein", "db_xrefs": [ "COG:COG0236", "COG:I", "RefSeq:WP_020002664.1", "SO:0001217", "UniParc:UPI0002DDE617", "UniRef:UniRef100_A0A2K9YT00", "UniRef:UniRef50_A0AA43R1D2", "UniRef:UniRef90_A0A2K9YT00" ], "nt": "ATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAATTTAATAAAGAAACTTTTATTCGTGATTTAAATATTGATAGTTTAGATTTGGTTATACTTGTTAGTGATTTAGAAGAGGCTTACAATATAAAAATAAGTGATGAAGAATTGCTTTCATTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAA", "aa": "MESEKIIINAIKKLTKTKFNKETFIRDLNIDSLDLVILVSDLEEAYNIKISDEELLSLKTINDIIELFDKKTNK", "aa_hexdigest": "0fda9036b020adeab1537197b28e01cc", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0002DDE617", "ncbi_nrp_id": "WP_020002664.1", "uniref100_id": "UniRef100_A0A2K9YT00", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002DDE617", "RefSeq:WP_020002664.1", "UniRef:UniRef100_A0A2K9YT00" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YT00", "uniref90_id": "UniRef90_A0A2K9YT00" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YT00", "gene": "AcpP", "product": "Acyl carrier protein", "uniref50_id": "UniRef50_A0AA43R1D2", "cog_id": "COG0236", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_A0AA43R1D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43R1D2" ], "product": "Acyl carrier protein" }, "genes": [ "acpP" ], "id": "GLJLFKCOHC_380", "locus": "GCA013368035_00353" }, { "type": "tRNA", "sequence": "contig_1", "start": 196943, "stop": 197018, "strand": "+", "gene": null, "product": "tRNA-Xxx", "score": 66.8, "nt": "ATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCA", "db_xrefs": [], "id": "GLJLFKCOHC_15", "locus": "GCA013368035_00354" }, { "type": "tRNA", "sequence": "contig_1", "start": 197124, "stop": 197198, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(ggt)", "amino_acid": "Thr", "anti_codon": "ggt", "score": 76.0, "nt": "GCCGTCATAGCTCAGGGGCAGAGCACTTCCATGGTAAGGAAGGGGTCGTTGGTTCAATTCCAATTGACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 197164, 197166 ], "id": "GLJLFKCOHC_32", "locus": "GCA013368035_00355" }, { "type": "cds", "sequence": "contig_1", "start": 197308, "stop": 198387, "strand": "+", "frame": 1, "gene": "prfA", "product": "peptide chain release factor 1", "db_xrefs": [ "COG:COG0216", "COG:J", "GO:0006415", "GO:0016149", "KEGG:K02835", "RefSeq:WP_012855437.1", "SO:0001217", "UniParc:UPI0001BC213B", "UniRef:UniRef100_D1J7U7", "UniRef:UniRef50_A5IZH6", "UniRef:UniRef90_D1J7U7" ], "nt": "ATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATACGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAGATTTATGATGCTTTTAAAAAATACCAAAACATTGAAGAATTATATTTCTTGTCAAAAGAAATTTTAACAAACGAAAAAGATCAAGAATTAATTGAACTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGTGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATCAAAGGTTTTAAAATTAAAATAATTGATTCTACATATGGCGCAGCTGGTGGATATAGTCAAATTGTTTTTAGTATAAAAGGCGAAAAGGTTTTTTCACAACTAAAATTTGAACGTGGAGTGCATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGACGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCTGAAGTTGATGAAGATGTAAAAATTGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGTTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCGACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCCTTAAAAGTTTTAAAAAGTAGATTGTATGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAACTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTATCCTCAAGATAGAATTACAGATCATAGAATTGGCTTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATCATTGAAGCAGGAAATTAA", "aa": "MEKTMYDSLLSIKNQYDELTEKLNDPNIYNDIKAYTRIAKEKSELQEIYDAFKKYQNIEELYFLSKEILTNEKDQELIELAKNDIDNQMNQLQEIEEEIKELLLPQDENDKRDVIMEIRGAAGGDEANIFSGDLFKMYQKYAEIKGFKIKIIDSTYGAAGGYSQIVFSIKGEKVFSQLKFERGVHRVQRVPATETQGRVHTSTATVTVLPEVDEDVKIEIRPEDIEVNVFRSSGAGGQSVNTTDSAVRITHKPTGLVVTSQDERSQIQNKETALKVLKSRLYELELRKREEAESGLRKLAGTGDRSEKIRTYNYPQDRITDHRIGFSTSLKVVMEGELDKIIEALIADEKAKKIIEAGN", "aa_hexdigest": "bf620fc07af778f8d328264bc301b096", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC213B", "ncbi_nrp_id": "WP_012855437.1", "uniref100_id": "UniRef100_D1J7U7", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC213B", "RefSeq:WP_012855437.1", "UniRef:UniRef100_D1J7U7" ] }, "ips": { "uniref100_id": "UniRef100_D1J7U7", "uniref90_id": "UniRef90_D1J7U7" }, "psc": { "uniref90_id": "UniRef90_D1J7U7", "gene": "prfA", "product": "peptide chain release factor 1", "uniref50_id": "UniRef50_A5IZH6", "cog_id": "COG0216", "cog_category": "J", "kegg_orthology_id": "K02835", "go_ids": [ "GO:0006415", "GO:0016149" ] }, "pscc": { "uniref50_id": "UniRef50_A5IZH6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5IZH6" ], "product": "Peptide chain release factor 1" }, "genes": [ "prfA" ], "id": "GLJLFKCOHC_381", "locus": "GCA013368035_00356" }, { "type": "cds", "sequence": "contig_1", "start": 198387, "stop": 198779, "strand": "+", "frame": 3, "gene": null, "product": "methyltransferase domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "nt": "ATGATAGATAAAGAAATTTTGCTACGTGAAAAAGAAAGATATAATTTACCCCTATATATTTCAAAAAAAGAATTAAGAATGCTAAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTAAATTTAAAATATAAAACCTTGATTCCTAGATATGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTAGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGACGCTAATGTTACATTAAGTGATATTAGCTGTTTTGCAATCAAACAAACAAAAATAAATGCCAAATTAAATAATTTAAATGTGAATGTAATTCGTTCAAATTGA", "aa": "MIDKEILLREKERYNLPLYISKKELRMLKKDVPVQKIIGYQEFQNVILNLKYKTLIPRYETEEVILAAYPFIKKESKVLDLGCGSGLIGLAIKKNKDANVTLSDISCFAIKQTKINAKLNNLNVNVIRSN", "aa_hexdigest": "f875c640b65eba291c2f8227d3ce2b3a", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0022080B54", "query_cov": 0.8846153846153846, "subject_cov": 0.9274193548387096, "identity": 0.513, "score": 94.4, "evalue": 1.82e-22, "valid": false, "product": "methyltransferase domain-containing protein", "uniref50_id": "UniRef50_UPI0022080B54" }, "pscc": { "uniref50_id": "UniRef50_UPI0022080B54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0022080B54" ], "product": "methyltransferase domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_382", "locus": "GCA013368035_00357" }, { "type": "cds", "sequence": "contig_1", "start": 198885, "stop": 199094, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCGTTTAATTATTTAAACGAAAAAGGAATAATTATTTTTGAGGTCGATCCTTATGTTTCTTTATGGTTTAAAAATAAATTTCCAAATGTTCTAATATTAAAAGATATAAATAACAAAGATAGAATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAG", "aa": "MFARDNGLFAYKKILKSAFNYLNEKGIIIFEVDPYVSLWFKNKFPNVLILKDINNKDRIALLAKNDLKN", "aa_hexdigest": "f98bab9733468f9460d6fdb3fdfbcf6a", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8108.458099999998, "isoelectric_point": 9.646807289123537 }, "id": "GLJLFKCOHC_383", "locus": "GCA013368035_00358" }, { "type": "cds", "sequence": "contig_1", "start": 199129, "stop": 200241, "strand": "+", "frame": 1, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "db_xrefs": [ "COG:COG0484", "COG:O", "EC:1.8.4.-", "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408", "SO:0001217", "UniRef:UniRef50_A0A448ZV77", "UniRef:UniRef90_D1J7U9" ], "nt": "ATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATATCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATACGAAGTTTTATCTGATCCTGAAAAAAGACAAAAATATGATAAATTTGGACATTCTGCATTCGATCAAAGTTCATTTGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCGAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGTTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTTTAGATTCAATATTTGGAAAAAATGTAACTGTTCAATTAAAGAAATATAGCGAATGTTCACATTGCCACGGTTCAAAAGCAGAAAGTGATAGCGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTTTTTTCAACAATTAGTCCTTGCAACAAGTGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGATATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGTGGAATTAACAATGGCGAATATATTAAATTGTCAGGATACGGTAATCCTTCTCGTAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACTATTAATGTTCCTACACCATACGGAAATAAAGATATAAAATTAAATAAAAATATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATTACATTAAAGCCTTATATTCCTTCGCTTAATAAAGAAGATAATAAAAAAGTTCAAGAAATATTTAGCAACTATAAAGATGTAGAATTTCAAAAATGA", "aa": "MENKRDYYEVLGISKNATEKEIKSAYRKLAMQYHPDRNKAPDAEEKFKEVSEAYEVLSDPEKRQKYDKFGHSAFDQSSFGYSEDVFNSFFNSFRDMFSGGGFEVDSSDDSDDPFSSIFGFGSNRSRKTKGSDLQMKLSIDFLDSIFGKNVTVQLKKYSECSHCHGSKAESDSDIVSCPQCHGSGQVQQKMGFFSTISPCNKCGGLGKSIKNTCHVCSGKGYEYKLVNENIQIPGGINNGEYIKLSGYGNPSRNGGSNGDLYIIIAIRPHKFYERFNNDIVVSMPISVFSIINEDTINVPTPYGNKDIKLNKNMKLDGVITLDGYGFPIKNSSKKGNFIITLKPYIPSLNKEDNKKVQEIFSNYKDVEFQK", "aa_hexdigest": "c0b34048477360514b7509298147c057", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7U9", "query_cov": 1.0, "subject_cov": 0.9814323607427056, "identity": 0.995, "score": 732.0, "evalue": 1.32e-266, "valid": true, "gene": "dnaJ", "product": "molecular chaperone DnaJ", "ec_ids": [ "1.8.4.-" ], "uniref50_id": "UniRef50_A0A448ZV77", "cog_id": "COG0484", "cog_category": "O", "go_ids": [ "GO:0005515", "GO:0005524", "GO:0006457", "GO:0009408" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZV77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZV77" ], "product": "Chaperone protein DnaJ" }, "genes": [ "dnaJ" ], "id": "GLJLFKCOHC_384", "locus": "GCA013368035_00359" }, { "type": "cds", "sequence": "contig_1", "start": 200427, "stop": 200582, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAATAAAATCATGATTGTTGAATCACCAAATAAGGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAAGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATGGGGGGATTAGGTATAGATTTTGAAAATTGA", "aa": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGMGGLGIDFEN", "aa_hexdigest": "4d5cd129eadb00eb03c9cdde5b69bb8e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5502.407799999998, "isoelectric_point": 6.527242088317871 }, "id": "GLJLFKCOHC_385", "locus": "GCA013368035_00360" }, { "type": "cds", "sequence": "contig_1", "start": 200592, "stop": 201095, "strand": "+", "frame": 3, "gene": null, "product": "DNA topoisomerase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "nt": "ATGGTAATTGATCCAAGCAAGAGAAAAATCGTATCTGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGCGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAGATAAATATAAAAGAGTGAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGATAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATTGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTATTCCAACATTATATTCAAAAATTGAAGCACATACTGCTGATGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGTGAAAATGAATGA", "aa": "MVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFIPTLYSKIEAHTADEQIANFVYRDNLDFVGENE", "aa_hexdigest": "a7316725fddc52d6a5022b04ae39801b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002FF0FCFB", "query_cov": 0.8203592814371258, "subject_cov": 0.8726114649681529, "identity": 0.547, "score": 138.0, "evalue": 7.02e-39, "valid": false, "product": "DNA topoisomerase", "uniref50_id": "UniRef50_UPI002FF05F56" }, "pscc": { "uniref50_id": "UniRef50_UPI002FF05F56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FF05F56" ], "product": "DNA topoisomerase" }, "genes": [], "id": "GLJLFKCOHC_386", "locus": "GCA013368035_00361" }, { "type": "cds", "sequence": "contig_1", "start": 201150, "stop": 202034, "strand": "+", "frame": 3, "gene": "topA", "product": "type I DNA topoisomerase", "db_xrefs": [ "COG:COG0550", "COG:L", "EC:5.6.2.1", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265", "KEGG:K03168", "SO:0001217", "UniRef:UniRef50_B3PMD8", "UniRef:UniRef90_D1J7V0" ], "nt": "TTGCAGGTAATAGACTTTAAAAAATCTATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTATATAAAGAATCTCAATATAGTTCAGCAACCGTTCAAATTGCTGCACAAAAATTATTTGAAATGGGGTTAATTTCTTATCCTAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCCCAAAAATATGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTGCTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACACCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTCAAAATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTATTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTGGAATTTGCTAAATTAGACAAAGAAACTAAAGCTCCTGCAAGATATAATGATGGTTCGTTAATTAAGATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACGACAGTTAAAATTATAAAAGATAGATTATTTGTTGAAACTAAAAATAATAGTTTATATCCGACAGATTATGGAAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATTAATGAAGAATATACAGCTAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGATTATCAGATTATAAAATTTTATTGAAAGATTTTTGA", "aa": "MQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFAKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKILLKDF", "aa_hexdigest": "eb72ff45cd1c84d9375bb1295a8439ce", "start_type": "TTG", "rbs_motif": null, "genes": [ "topA" ], "truncated": "both", "pseudogene": { "start": 200850, "stop": 202278, "observations": { "insertion": [ 202239 ], "mutation": [ 201093, 202032 ], "stop": [ 201093, 202032 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V0", "query_cov": 1.0, "subject_cov": 0.4772727272727273, "identity": 0.99, "score": 567.0, "evalue": 3.13e-199, "gene_start": 1, "gene_end": 294, "reference_start": 242, "reference_end": 535, "reference_sequence": "MENKIMIVESPNKVNTIQKIVGDNIKVLSSIGHILKMSTSGIGGLGIDFENWEPKMVIDPSKRKIVSELKEAAKNASEVLIATDPDREGEAIANNLVNTLKVQDKYKRVKYNEITPDAINYAIENPLTIDENLVNAQKARRILDRIIGFKLSQLIQRKVKNTPTNPSAGRVQSIALKLVVDKEEEIAKFVPTLYSKIEAHTADEQIANFVYRDNLDFVGENEWIKREKVDDILAQLNQNKSLQVIDFKKSIRSEAMVVPFKQSILYKESQYSSATVQIAAQKLFEMGLISYPRTDSTRLSSIFIEKSQKFIAQKYGKEYIATEIKGFSGDQDAHEAIRPTDISLLASEAQQKFNLSNEETNVYKLIYEKTLMALMATPKREIYQYELKNGQYNFKMSYSKIIFDGYYKIFDKKQESIIPNYKVGDIIKVLEFVKLDKETKAPARYNDGSLIKMLDDIKVGRPSTFATTVKIIKDRLFVETKNNSLYPTDYGKLVLEKLIQGFPTTINEEYTASVENQLDLISEGLSDYKVLLKDFWNNFNLQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFLKEDIINNFYF" }, "paralog": false, "identity": 0.9894736842105263, "score": 921.0, "evalue": 0.0, "description": "Internal stop codon at 201093, 202032. Frameshift due to insertion around 202239. Nonsense mutation around 201093, 202032.", "psc": { "uniref90_id": "UniRef90_D1J7V0", "gene": "topA", "product": "type I DNA topoisomerase", "ec_ids": [ "5.6.2.1" ], "uniref50_id": "UniRef50_B3PMD8", "cog_id": "COG0550", "cog_category": "L", "kegg_orthology_id": "K03168", "go_ids": [ "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V0", "EC:5.6.2.1", "UniRef:UniRef50_B3PMD8", "COG:COG0550", "COG:L", "KEGG:K03168", "GO:0003677", "GO:0003917", "GO:0005737", "GO:0006260", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMD8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMD8" ], "product": "DNA topoisomerase 1" } }, "id": "GLJLFKCOHC_387", "locus": "GCA013368035_00362" }, { "type": "cds", "sequence": "contig_1", "start": 202047, "stop": 202265, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01396.24" ], "nt": "TTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTCTTACCTCAAGAAATTTTGCAAGAAAAATGTCCTCTTTGTGGACATGACTTGCTTTATAGATATACTAAATCAAAACACCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATTGACAATGAAAAACCTAAATTTTTTAAAAGAAGATATAATAAATAA", "aa": "MQLQNVNETMETDFLPQEILQEKCPLCGHDLLYRYTKSKHQKFIGCSNFPSCRYIRNIDNEKPKFFKRRYNK", "aa_hexdigest": "e7c9e3d77905d61d1e4033d4c4c50f55", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01396.24", "name": "Zn_ribbon_Top1", "length": 40, "aa_cov": 0.5555555555555556, "hmm_cov": 0.95, "evalue": 8.78051824668985e-17, "score": 56.109832763671875, "start": 21, "stop": 60 } ], "seq_stats": { "molecular_weight": 8776.033999999992, "isoelectric_point": 9.182634925842287 }, "id": "GLJLFKCOHC_388", "locus": "GCA013368035_00363" }, { "type": "cds", "sequence": "contig_1", "start": 202583, "stop": 202726, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTATTTACAATATTGATATTTATTCAAAAACTGGAAGACCAAATACTAACGATTTAGACTTAAGAAATAAAGACAATATTTTTAAGGGCTATAAAGATGTTACATTTGAATTCAGTACCGAAAATGTTCCACCTAGATGA", "aa": "MTIYNIDIYSKTGRPNTNDLDLRNKDNIFKGYKDVTFEFSTENVPPR", "aa_hexdigest": "78ee2311cc1a6af6ddc988c7f337a800", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5542.1091000000015, "isoelectric_point": 6.049965095520019 }, "id": "GLJLFKCOHC_389", "locus": "GCA013368035_00364" }, { "type": "cds", "sequence": "contig_1", "start": 202850, "stop": 206419, "strand": "-", "frame": 1, "gene": null, "product": "membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000371E367", "UniRef:UniRef90_UPI002410F427" ], "nt": "ATGCAATCAGCAAAAGAATCTCTAGATGCCAAAGTTACTGAAATTACTAAAAAATTAGAAACATTTAATAAAGATAAAGATGCTAAATTCAATGAATTAGAACAAACAAGAAAAGACATTGATGAATTTATTAATTCAAATAAGGATAATTCAACTTATTCAAACATAATTTCAAAATTAACCACGACTAAAAATGAAAAAGATTCAGTTTCTAAATCTTCAAATAAATCAGATATTGAAGACACAAACACTTTGCTAAAAAATTCTTTACAAGAAGCAAATGATAAAAAACAAGAAATTGATAAATTCGTTTTAGCTAAAAAAGAATTAGAAGAATTTATTAAAAATGATGAAGATGCTAAAAGTGATGAATTAGTTTCAAATTTAAACGATGCTAAACAAGAATTAGCAAAATTTAGTGGCATAGATGATTCACGCGAAATCACTGAAATTAAAAAAGCTGCTGATGATTTAACAAATGCAAAAACAAAATTAAAAGAAGAAATTAAAAACAAAAAACAAGAGTTGTTTAATGCATTTGAATCATCTAAAACAGCATTGCAAAACTACATTACAAACGATATTAAAGATTCAAAATATAAAATTATTCAAGATAAATTTAATAATGTTATTGATGAATATAAAAACATTACATCAAACGACAAAATTTCAAAAATTAAAGAAGCTACAAATAATTTAAATGCAAAATTTACAGAAATTGGAAGCAACAAGGCAATATTAGATAAGTTTTATGAATTGAAGTCAAAACTTGAAGATTTATTAAAAACTGCAAATCTTAAAGATGATGCAAAATCCGAAAATATTGATTTATCTTCTGAAGAGCAAGCTTCTACTGATGCCAAGGCTATAAATAATAGTTCAACTATTGAAAATGTTGAACAAGTTAACCAAAAATTGCAAGCAGCAATAGATAAATTAACTAAAGATATTCAAAAAATTAATGAAGATAAAAAAGCTAAATTTGAAGAATTTAAAACAGAAAAACAACATCTAGAAAACATAATCAAAACCAAAGGTTTAGATAAATTTATTAATATTGATGATGAAATAAAATCAATAAAACAACAAATTGATGAAGAAATTAAAAAACACAAAATAGACAAAAATTCAAAAATTTCTGATATTTTAGCAGCAAAAGATAGTATCGATCAAACACAAAGAAAACTATTAGGTTTATTGGATGATTATTCAACTCAAAGAACAGAATCTATAGACAATTTCAATAAGCAAAAAACCGAATTAGATTCTTTGATAAATTCTAATAATAATTTTAAACAATTAGAAGAATCTAAAAAAACTGAATTTAATAATAAAAAACAAGAATATGAAGTCTTGGATTTAGAAACATTAAGTAAGAATGAAATTCAAGAAAAATCAAAAGAAATTAAAAAATTAATAGATAATATAAATAAAGAAATCTCAGATGCCAAAAAACAAGAATATTCAAAATTTGATAAAGCAAAAAAAGAATTAGAAAAATACGTTAAAGATGAATTAAATAATCAAAAATACGATTCTCTTAAAAAAGAAGCACAAAATAAAATAAATAGTTTTAGTGCAATAAAAGAAGAATCTTTAGCAACTAAAGAAATTCAAGAAATTAAAGATGCAACAGCTGCATTAATCAAGGCTAAAACTAACGCCGAACAAGAAAAAGCAAAAATTGATGCCAAAGAACAACTAAATGCAAAAATTCAAGAGGCAGATAAACTTAAAAAAGAATTAATCGATAGTGATCCTACAATAACTACGCTAAAAACTAATTTGGAAAAAGAAATTGCTAAAGCTAACGAAGCACTTAAAAAAGATACTAAAGCAATTATTGAAGCAAAAGAACAATTAGCAAACAAAATTGATGAATTTAAATCAAGTCTTGACAAGTTTAATAAAGAAAAAGGAACAAAGTTTAAAGAATTAGAAAAAACAAGAAAAGATATTAATGATTTCTTAACAAATGATGTTAAAACCAATCCAAATTATGCTGATCTTGTTAAAAAATTACAAAATTCTTTAAAAACGCAAGAAAAAATTAACAACATTTCAAACAAAGCAAATATTATTGCTGCAAATAATGAATTAACTAATGCATTGAAAGAAGCAAAAGAGGCAAAAAAACAAGCGGATAGAGCAAATAGCGAAGCTAATAATAAATTAACCACCAGTTTATCTACTGCAAACGAATTATTAAAAAAATTAACTGATTCAGATACTGACATTCAAAATTATAAAAAACAACTTGAAGAAGAAATAAATAACGCAAATAAGGCAATTACTTCAAAAAATACAAAATCATTGACTGATTCAAATAATTCATTAACAAATAAAACTAATGAAATTCAAGAAAAACTTAATAAATTCATAGAATCAAAAAATAAAGAATATGAAAAATTAGAACAAACAAGAAAAAATATTGATGAATTCATAAAACAAATTGAAAATGTTCCTCAAACTAAAAACAATTGTCAAAATATAATTAAAAACTTAAAAAATAAAAAAATTGCTAAAGATTCAATAACTATTTCTAATAATAAAAAAGAAATTCAAGATGCAAATCAATCATTGCAAAATGAATTAAATAATGCAAAGAATACAAAAGAAGATATTACTAATTTTTACAACAGCAAAAAGCAATTGGAAAATTTGATAAAAACTGATGATGCTAAAAAAGCAGGAACTGCAGAAGCAGATACAATTTTAAAAAATTATAAAAATATATCGGATGCTTCAAAAGATGAAGAAATTAAACAAGCAACCCAAAAAATAAATGATATTAAAAAAATAATAGAAAATAAAATTGAAGAAAAAAGACGCAGCGAATTTTCTCAATTTGAACAAATAAAAAATGAGTTGCAAAATTTTATTAATAAAGATTTGAAGGATCAAAAATATAATTCAATTAGAACAAAAATCGAAAATAAAATAAATGGTGTTTCAAGTATAAATAAAAATTCAAAAATTCAAGATATAGAAAATGCTAAAAAACAATTAGAACCTGCTAAAAACGAGTTTGAAAAAATAATAAATAATATTAAAGAATTTAATTCTCAAAAACAAAAAATTGAAGAAAAGTTAAAATTTTTAGATACTGAAAACATTGGTACCTTAGGTAGTCTTTCAAACGAAAACAAAGGTATAAAGGCAATTTTTCAAGGTTTCATAAACGAAATAGAAAAAGAATTAATTAATAAAAATGCTGACGGTAAGGTAAAAGATGAATGCATTAATAAAATAGATTTTTTAGACAAAGCTTTAATTTCAAGATATGAAGGAATTTTAAAAGCAGCATATAAATTTTTCGACGATACATCAGTACAAAAGAATGAAGCAGAAAAAAATGAATTTTTTGATAATTTAAAAAATAATTTAAGTCAAGATAAAGTTTTTGACCAACTAGAAAAAATGAATATGAATCATTTTGAAGATTTTTATAAAACATTCTATAATAAAGGCAATGTGCCATTAAAAGATGGTAAGCCAAATTATGATGATCCAAAATATAAAGAAGAAATTAACAAACAAAATGAATTAGAAGGGCCAGCTGTAAAGTGA", "aa": "MQSAKESLDAKVTEITKKLETFNKDKDAKFNELEQTRKDIDEFINSNKDNSTYSNIISKLTTTKNEKDSVSKSSNKSDIEDTNTLLKNSLQEANDKKQEIDKFVLAKKELEEFIKNDEDAKSDELVSNLNDAKQELAKFSGIDDSREITEIKKAADDLTNAKTKLKEEIKNKKQELFNAFESSKTALQNYITNDIKDSKYKIIQDKFNNVIDEYKNITSNDKISKIKEATNNLNAKFTEIGSNKAILDKFYELKSKLEDLLKTANLKDDAKSENIDLSSEEQASTDAKAINNSSTIENVEQVNQKLQAAIDKLTKDIQKINEDKKAKFEEFKTEKQHLENIIKTKGLDKFINIDDEIKSIKQQIDEEIKKHKIDKNSKISDILAAKDSIDQTQRKLLGLLDDYSTQRTESIDNFNKQKTELDSLINSNNNFKQLEESKKTEFNNKKQEYEVLDLETLSKNEIQEKSKEIKKLIDNINKEISDAKKQEYSKFDKAKKELEKYVKDELNNQKYDSLKKEAQNKINSFSAIKEESLATKEIQEIKDATAALIKAKTNAEQEKAKIDAKEQLNAKIQEADKLKKELIDSDPTITTLKTNLEKEIAKANEALKKDTKAIIEAKEQLANKIDEFKSSLDKFNKEKGTKFKELEKTRKDINDFLTNDVKTNPNYADLVKKLQNSLKTQEKINNISNKANIIAANNELTNALKEAKEAKKQADRANSEANNKLTTSLSTANELLKKLTDSDTDIQNYKKQLEEEINNANKAITSKNTKSLTDSNNSLTNKTNEIQEKLNKFIESKNKEYEKLEQTRKNIDEFIKQIENVPQTKNNCQNIIKNLKNKKIAKDSITISNNKKEIQDANQSLQNELNNAKNTKEDITNFYNSKKQLENLIKTDDAKKAGTAEADTILKNYKNISDASKDEEIKQATQKINDIKKIIENKIEEKRRSEFSQFEQIKNELQNFINKDLKDQKYNSIRTKIENKINGVSSINKNSKIQDIENAKKQLEPAKNEFEKIINNIKEFNSQKQKIEEKLKFLDTENIGTLGSLSNENKGIKAIFQGFINEIEKELINKNADGKVKDECINKIDFLDKALISRYEGILKAAYKFFDDTSVQKNEAEKNEFFDNLKNNLSQDKVFDQLEKMNMNHFEDFYKTFYNKGNVPLKDGKPNYDDPKYKEEINKQNELEGPAVK", "aa_hexdigest": "89498b92a727a02a9da0d22b611421a7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002410F427", "query_cov": 0.8528174936921783, "subject_cov": 0.8615123194562447, "identity": 0.9990000000000001, "score": 1530.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_UPI000371E367" }, "pscc": { "uniref50_id": "UniRef50_UPI000371E367", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000371E367" ], "product": "membrane protein" }, "genes": [], "id": "GLJLFKCOHC_390", "locus": "GCA013368035_00365" }, { "type": "cds", "sequence": "contig_1", "start": 206447, "stop": 208501, "strand": "-", "frame": 1, "gene": "smc", "product": "Lmp3 protein", "db_xrefs": [ "COG:COG1196", "COG:D", "SO:0001217", "UniRef:UniRef50_D1J7V1", "UniRef:UniRef90_D1J7V1" ], "nt": "ATGAAAAAAAGAATAAACATTTTAATGTCAATTTCAATCGTTTGTGCTGCTGCATCAACGTCAGCTATTGCTGCTATGTGCTCGATAAGCAATAACTCTACGAAAAAGTATAACGAAGCAAAATCTAGATTACTTAATCTGATTAAGAAACTAGATAATGAAGGTCAAAAGAAAGCAAATGATTTTATTGCAAGACAAGATAAGAAATTTAATAGTACAGCATTTAAGAATCATTCAAACACATCAAAACTTATTGATGAAATAGAAATTTTTAGTAAAAAGATATTAGAAAATCTACAAAAAGATGAAAAACAAAGATTAGAAGAATTAAATAAATTAAATAAATTAAGATTAGATTTACAAAACTTAATTAATTCAAATGACGGTCAAAACGTCGATTCTTCTGATGCTGAAAAAGCATTAAAAGAAAATCAAATAGATGATTCTTTGCCAATAGATAAAATTAAAAAGACAAATGAAAATCTTGAAAATGCAAAAAAAGAACTTCTAAATAAAATAAATACCGAAAGAGAATTACAATCAAAAATTTTTAATGATAAAAAACAAGAATTAAAAAGAGTGCTTGATTTAGAAGATGCAAAAGAAGTAGATTTTATAAAAGAACAAAAAGTTTTTGTTGAAACAAATATAAGTGAAACATCATCAATTGAAGATATAAAAAACAAAATTATTGAAGTTGAAAAAGCAACTTCTTCTTTAACATCAAAAATTTTAAATACCAAACAACAAGAACTTCAAAGATTTGAAAATATTAAAAAAGACCTACAAGATTTTATTAACACAAAATTAAATGAAGCAAAATATCAATCAATAAAACAAAAGGCTTTAGATAAAATAAATAGTTTAAATGGAATAAATAAAGATTCAACAATTAAAGAAATAAAAGCTGGGCAAAATGCTTTAATAAAAGCAAAAGAAGAAGCGGGCCTTGAAAAAGAAAAATTAGATGGTCAAAATATAAAAGATACTCTTAAAAAAACTATAAATAATGCCAAGGAATTTAAAAAGTTATTAATTGACAATGACCAAAAAATAGTTGATTTAAAATCTAATTTAGATAATGAAATTTCTAAAGCAGAACAATCACTTTCAAAAGACAAAAAATCAATGGAATCAGCCAATGATTTATTGAATACTAAACTTATTGAATATAAAGAAATCTTAAATAAATTCAATCAAGAAAAAGAAGCAAAATTCACTGAATTAGAACAAACAAGAAAGAATATTGACAATTTCTTAACAGATGACGTTAAAAATAATCCAAATTATGCAACTTTAGTTAAAGATTTAGCCAATGCAAAAGATGCTAAAAAATCATTAACTAAATCTTCAAATAAATCAGACATTATTGCCGCAAATGAAGCATTAATACAAGCATTAGCAAATGCTAATAAAGCAAAAGATCAAGTTGATGATGCAAATAAATCAATTAAAGAACAATTGAATGCATTAATTGACAAAGCAAACACATTATTGCCTCAATTAAATGATAATGATAGTGAAATTGTAAAAGCTAAAGAATCATTAAATGTAGAAATTACCAATGCAAATAAAGCAGTTAACCAAAATGACAATGCTTCAATGCAATCTGCTAAATCTTCATTGGATGCCAAAATTACTGAAATTACTGAAATTACTGAAAAATTAGAAACATTCAATAAAGATAAAGATGCTAAATTTAAAGAATTAGAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACCAATCCAAATTATGCAGATTTAATTGCAAAATTAACTGATGCCAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGAAATTATTGCCGCAAATGAAGCATTAAAACAAGCCCTTGAAAAGGCAAAAGCAGCAAAAGATCAAGTTGATGAAGCAAATAAATCAATTAAAGAACAATTAAACGATTCAATTACTAATGCAAACCAATTATTAAATAAATTAGTTGATTCAGACAAAGACATTCAAAAAGCAAAAACTGAATTAAGTCAAGAAATTCAAAGTGCAAGTTAA", "aa": "MKKRINILMSISIVCAAASTSAIAAMCSISNNSTKKYNEAKSRLLNLIKKLDNEGQKKANDFIARQDKKFNSTAFKNHSNTSKLIDEIEIFSKKILENLQKDEKQRLEELNKLNKLRLDLQNLINSNDGQNVDSSDAEKALKENQIDDSLPIDKIKKTNENLENAKKELLNKINTERELQSKIFNDKKQELKRVLDLEDAKEVDFIKEQKVFVETNISETSSIEDIKNKIIEVEKATSSLTSKILNTKQQELQRFENIKKDLQDFINTKLNEAKYQSIKQKALDKINSLNGINKDSTIKEIKAGQNALIKAKEEAGLEKEKLDGQNIKDTLKKTINNAKEFKKLLIDNDQKIVDLKSNLDNEISKAEQSLSKDKKSMESANDLLNTKLIEYKEILNKFNQEKEAKFTELEQTRKNIDNFLTDDVKNNPNYATLVKDLANAKDAKKSLTKSSNKSDIIAANEALIQALANANKAKDQVDDANKSIKEQLNALIDKANTLLPQLNDNDSEIVKAKESLNVEITNANKAVNQNDNASMQSAKSSLDAKITEITEITEKLETFNKDKDAKFKELEQTRSQIEAFINANKTNPNYADLIAKLTDAKKAKESVSESSNKSEIIAANEALKQALEKAKAAKDQVDEANKSIKEQLNDSITNANQLLNKLVDSDKDIQKAKTELSQEIQSAS", "aa_hexdigest": "1da4acf1281525c63facf4fe4b321ee7", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "genes": [ "smc" ], "truncated": "5-prime", "pseudogene": { "start": 206165, "stop": 208501, "observations": { "insertion": [ 206596 ], "deletion": [ 206457 ], "mutation": [ 206448 ], "stop": [ 206448, 206475, 206517, 206526, 206559 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V1", "query_cov": 1.0029239766081872, "subject_cov": 0.43144654088050316, "identity": 0.873, "score": 956.0, "evalue": 0.0, "gene_start": 1, "gene_end": 684, "reference_start": 1, "reference_end": 682, "reference_sequence": "MKKRINILMSISIVCAAASTSAIAAMCSISNNSTKKYNEAKSRLLNLIKKLDNEGQKKANDFIARQDKKFNSTAFKNHSNTSKLIDEIEIFSKKILENLQKDEKQRLEELNKLNKLRLDLQNLINSNDGQNVDSSDAKKALNENQIDDSLPIDKIKKTNENLENAKKELLNKINAERELQSKIFNEKKQELKRVLDLEDTKEVDFTKEQKVFIETNINETSSIEDIKNKIIEVEKATSSLTSKILNTKQQELQEFENIKKDLQDFINTKLNDAKYQSIKQKALDKINSLNGINKNSTIKEIKAGQNALIKAKEEAGLEKEKLDGQNIKDTLKETINNAKEFKKLLIDNDQKIVDLKSNLDNEISKAEQSLSKDKESMESANDLLNTKLIEYKEILNKFNQEKEAKFNELEQTRKNIENFLTDEVKNNPNYATLVKDLTNAKDAKKSVTNSSNKSDIIAANEALIQALADANKAKDQVDEANKSIKEQLNALIDKANTLLPQLNDNDSEIVKAKESLNAEITNANKAVNQNDNASMQSAKSSLDDKVTKIQNQLTEFNKDKDAKFKELEQTRKDIDNFLTDDVKNNPNYATLVKDLTNAKDDKKSVTKSSNKSEIIAANDELKQALDKAKVAKDQIDEANKSIKEQLSDSITNANQLLNKLVDSDKDIQKAKTELSQEIQSASQELNLNNPTSMQSAKESLDAKVTEITKKLETFNKDKDVKFKELEKTRKDIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKAQADNLAKSTKEQLNNSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDVKFKELEQTRKDIDEFINTNKTNPNYSTLISELTSKRDSKNSVTNSSNKSDIETANTELKQALAKANTDKDQADNLARSTKEQLNKSISSANTLLAKLTDKDNTIQQAKTELEKEVQKANQAVASNNTASMQSAKSSLDAKVTEITKKLETFNKDKDAKFKELEQTRKDIDEFIKQIENDPQTKKNYQNIVKNLKDKKAEKNSITFSNNKKEIQDANKSLQDELNNAKITKKGITDFYNSKKQLEDLIKTDDAKKVGTTEADTILDHYKNISDASKNEEIKQATQKINDIKKIIETKIQEKKRNEFSQFEQIKNELQSFINKDLKDQKYNSIRTKIENKINGVSSINKNSKIQDIENAKKQLEPAKNEFEKIINNIKKFNSQKQKVEEKLKFLDTKDIGTSGSLSNKNKDIKAIFQGFINEIEKELINKNADDKAKDECINKIIFLDKALISRYEGILNVAYKFFGDTSTQNNQTEKNEFFDNLRNNLVDQKVFDQLEKMNMNHFEDFYNTFYNKGNAPLKPDGNPNYNDPKYLEEINKQNELEGPAVKWSAFDDILDAAFVRTAQTSFENKCKNLFNDHKKDDERYGTINKTLNVYDIDIYSKTGRADANDLDLRNKDKIFKGHEYVTFKFSTENVPGRWQKNFKGLAKWDGSKNRYRFVGIVSINLKYEYNLIISTKGENYKKPLSLEYKLGSNNKGQNYNPIIIDKISGIN" }, "paralog": false, "identity": 0.841025641025641, "score": 1147.0, "evalue": 0.0, "description": "Internal stop codon at 206448, 206475, 206517, 206526, 206559. Frameshift due to insertion around 206596. Frameshift due to deletion around 206457. Nonsense mutation around 206448.", "psc": { "uniref90_id": "UniRef90_D1J7V1", "gene": "Smc", "product": "Lmp3 protein", "uniref50_id": "UniRef50_D1J7V1", "cog_id": "COG1196", "cog_category": "D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V1", "UniRef:UniRef50_D1J7V1", "COG:COG1196", "COG:D" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7V1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V1" ], "product": "Lmp3 protein" } }, "id": "GLJLFKCOHC_391", "locus": "GCA013368035_00366" }, { "type": "cds", "sequence": "contig_1", "start": 208948, "stop": 209457, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2", "UniRef:UniRef90_D1J7V2" ], "nt": "TTGCAAATTAGTAAGCATATAAAAATTGATCCGGTTGTAATTAATTCGGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACATCCTGAGTTAAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCCGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGACGCTCATAATTTCATTTTTTCAAAAATTTTAACAAATCAAACATTTATTGAAATGAAATTCTTAAAAATGAGATTTTCTAATTTGGAATTAGACACATCTGATAAAGAATCAAATAAACTAAAAGTAACATATGAAGTTTATTTGAATTATGAATATGCAACAGGAAGCTTCGAACTTAATAATGTTAAGGGCACTTCGCAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCCGAAAAGTGA", "aa": "MQISKHIKIDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFIEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEK", "aa_hexdigest": "31d5a33fa3ddd867dd815ca307078b39", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 208786, "stop": 209760, "observations": { "mutation": [ 208930, 209455, 209551, 209590, 209701 ], "stop": [ 208930, 209455, 209551, 209590, 209701 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V2", "query_cov": 1.0, "subject_cov": 0.49271137026239065, "identity": 0.976, "score": 326.0, "evalue": 2.46e-110, "gene_start": 1, "gene_end": 169, "reference_start": 55, "reference_end": 223, "reference_sequence": "MKNHSNTRRKHRSLAPLYYTLGSIAAAATIAGAIYFVVKNGRRVSKQYWSIEKFLQISKNIKLDPVVINSASAKEIYENFIHEKQKVDALRNKYINQHPELKPYLNNNTRTAFDFKFKGPKKSEIKKLLKDMPEEFDAHNFIFSKILTNQTFTEMKFLKMRFSNLELDTSDKESNKLKVTYEVYLNYEYATGSFELNNVKGTSQSKYYFKGNQTVKVISDSEKWNLNSKFANNLEKNLDDARKIIRIGIVKNDPKWTQSNEFREMIFKWFKRIFIENDAFPDTYSKDEYEIMPFNETSSGDDFVRWNPKKGLNNLTIKFFYVNKKNPDIKSPGRIKIFTILKV" }, "paralog": false, "identity": 0.9629629629629629, "score": 622.0, "evalue": 1.83e-229, "description": "Internal stop codon at 208930, 209455, 209551, 209590, 209701. Nonsense mutation around 208930, 209455, 209551, 209590, 209701.", "psc": { "uniref90_id": "UniRef90_D1J7V2", "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V2", "UniRef:UniRef50_D1J7V2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7V2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19898.4939, "isoelectric_point": 9.236917304992676 }, "id": "GLJLFKCOHC_392", "locus": "GCA013368035_00367" }, { "type": "cds", "sequence": "contig_1", "start": 209819, "stop": 210148, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175949824.1", "SO:0001217", "UniParc:UPI00158EB349", "UniRef:UniRef100_UPI00158EB349", "UniRef:UniRef50_D1J7V3", "UniRef:UniRef90_D1J7V3" ], "nt": "ATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTGAAAACTCTTTATTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCCAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTGCAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAA", "aa": "MEIINKDLEAKLKTFQKQNYKLQKICQYFKIDLKKLNLNEFLDFNKNSENSLLDLKQIKQIIAENQGDSNILEVLNMIKELESINKEKTANDETYKQDQILKKILSNLE", "aa_hexdigest": "d025e6620dbb887dfda4ae93f5914247", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158EB349", "ncbi_nrp_id": "WP_175949824.1", "uniref100_id": "UniRef100_UPI00158EB349", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EB349", "RefSeq:WP_175949824.1", "UniRef:UniRef100_UPI00158EB349" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EB349", "uniref90_id": "UniRef90_D1J7V3" }, "psc": { "uniref90_id": "UniRef90_D1J7V3", "uniref50_id": "UniRef50_D1J7V3" }, "pscc": { "uniref50_id": "UniRef50_D1J7V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7V3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12907.755799999984, "isoelectric_point": 6.357861900329589 }, "id": "GLJLFKCOHC_393", "locus": "GCA013368035_00368" }, { "type": "cds", "sequence": "contig_1", "start": 210219, "stop": 210413, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAACAGACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGA", "aa": "MKTQKRINIAIDGPAGVGKTIMSTMLAKTLGYKFLSSGSFYRVVAYNALQNRLDLNDEESINNA", "aa_hexdigest": "9d43ae697aa19f7caf7f9de94e3a7a66", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 54, "aa_cov": 0.84375, "hmm_cov": 1.0, "evalue": 1.9860874871194675e-17, "score": 59.0133171081543, "start": 9, "stop": 62 } ], "seq_stats": { "molecular_weight": 7040.021100000001, "isoelectric_point": 9.520900535583493 }, "id": "GLJLFKCOHC_394", "locus": "GCA013368035_00369" }, { "type": "cds", "sequence": "contig_1", "start": 210504, "stop": 210659, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "ATGGCCGCATCAGCAATAGCAAAATTTCCTTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATATAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTACCGCAGGCAGAGGTTAAATTCTTTTTGTGA", "aa": "MAASAIAKFPLIRAKVNKFIQSFGLKYKGIIVDGRDATYRILPQAEVKFFL", "aa_hexdigest": "022ecf965105dc92e068de5a1e762d7b", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 50, "aa_cov": 0.9803921568627451, "hmm_cov": 0.98, "evalue": 6.950588417938064e-12, "score": 40.899166107177734, "start": 2, "stop": 51 } ], "seq_stats": { "molecular_weight": 5745.824500000001, "isoelectric_point": 10.21090564727783 }, "id": "GLJLFKCOHC_395", "locus": "GCA013368035_00370" }, { "type": "cds", "sequence": "contig_1", "start": 210708, "stop": 210902, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02224.24" ], "nt": "TTGGGTTTGAAGGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTATAATGACACAAATAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAA", "aa": "MGLKADYNEVLEDIKIRDYNDTNRKFDPLKISEGSIVIDTSNMSIQENFDVMYKEVRKRLDDEK", "aa_hexdigest": "d8380e0f2c4f50bd1230179322d30ea0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02224.24", "name": "Cytidylate_kin", "length": 56, "aa_cov": 0.875, "hmm_cov": 1.0, "evalue": 1.1624244623907323e-12, "score": 43.43675994873047, "start": 2, "stop": 57 } ], "seq_stats": { "molecular_weight": 7587.4448999999995, "isoelectric_point": 4.679520225524903 }, "id": "GLJLFKCOHC_396", "locus": "GCA013368035_00371" }, { "type": "cds", "sequence": "contig_1", "start": 210892, "stop": 211035, "strand": "+", "frame": 1, "gene": null, "product": "GTP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "nt": "ATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCTGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGA", "aa": "MRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAI", "aa_hexdigest": "62f2c355d3a368a5bd3cf56b2c965ad1", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A7C7AIQ0", "query_cov": 0.9574468085106383, "subject_cov": 0.8035714285714286, "identity": 0.711, "score": 68.2, "evalue": 2.66e-14, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A7C7AIQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A7C7AIQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7C7AIQ0" ], "product": "GTP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_397", "locus": "GCA013368035_00372" }, { "type": "cds", "sequence": "contig_1", "start": 211234, "stop": 211773, "strand": "+", "frame": 1, "gene": "der", "product": "ribosome biogenesis GTPase Der", "db_xrefs": [ "COG:COG1160", "COG:J", "EC:3.6.5.-", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "KEGG:K03977", "SO:0001217", "UniRef:UniRef50_Q98RC1", "UniRef:UniRef90_D1J7V5" ], "nt": "TTGTTGGCAGTAAATAAACTTGAGGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCGCTTGGATTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAACTTATTAGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAACCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACCTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGGACAACGCGAGACTCTGTTTCATCTCTAATAAAAATTAATGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATTGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGA", "aa": "MLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVEPYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNK", "aa_hexdigest": "791ddb9546d5c1c4b98d7c71849984f2", "start_type": "TTG", "rbs_motif": null, "genes": [ "der" ], "truncated": "both", "pseudogene": { "start": 210934, "stop": 212076, "observations": { "mutation": [ 211033, 211771, 211849 ], "stop": [ 211033, 211771, 211849 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V5", "query_cov": 1.0, "subject_cov": 0.40589569160997735, "identity": 0.9890000000000001, "score": 340.0, "evalue": 4.24e-114, "gene_start": 1, "gene_end": 179, "reference_start": 116, "reference_end": 294, "reference_sequence": "MMRNNIVAIIGKPNVGKSTLFNKIINKRKSIVYDTPGVTRDRLYEDAIWTGKKFRVVDTGGITIEDEDFKKQIKLQAQIAIDEANVIVFLIDGKEPLTVEDYYVASLLRKSNKHVLLAVNKLEGSNVNYYDNSIYSLGFDEIFPISAIHGDGIGNLLDKIIEKLNFSDKDVESYFKLALLGKTNVGKSTLLNTLANEERSIVSNVEGTTRDSVSSLIKINGEIFEVVDTAGIKRKSKLTESVEHYALMRANQSIEDANLCLLVLDATEEVSHFSQNVIGIAYELKKPLILIVNKWDLIEKDTNTMEKYKKDLLKKVKFVDWSPIVFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE" }, "paralog": false, "identity": 0.9894736842105263, "score": 718.0, "evalue": 1.22e-265, "description": "Internal stop codon at 211033, 211771, 211849. Nonsense mutation around 211033, 211771, 211849.", "psc": { "uniref90_id": "UniRef90_D1J7V5", "gene": "der", "product": "ribosome biogenesis GTPase Der", "ec_ids": [ "3.6.5.-" ], "uniref50_id": "UniRef50_Q98RC1", "cog_id": "COG1160", "cog_category": "J", "kegg_orthology_id": "K03977", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V5", "EC:3.6.5.-", "UniRef:UniRef50_Q98RC1", "COG:COG1160", "COG:J", "KEGG:K03977", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RC1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RC1" ], "product": "GTPase Der" } }, "id": "GLJLFKCOHC_398", "locus": "GCA013368035_00373" }, { "type": "cds", "sequence": "contig_1", "start": 211861, "stop": 212214, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14714.11" ], "nt": "GTGTTTATATCAGCCAAAGATAATTTAAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAACAAAAACAAAAAAGAAGAGAGAGAATAA", "aa": "MFISAKDNLRINKLRDTIIQVKNNISRKINTNQLNDLMATAQMIRPASSINGRRLSITFARQIDGSIPTFLLFVNNVDLAHFTYLRYIENQIRENYDFSGTPIELVLRNKNKKEERE", "aa_hexdigest": "51debaa0839e8831ce52cb33b0871601", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14714.11", "name": "KH_dom-like", "length": 81, "aa_cov": 0.6923076923076923, "hmm_cov": 1.0, "evalue": 2.481795456492353e-28, "score": 93.66259002685547, "start": 28, "stop": 108 } ], "seq_stats": { "molecular_weight": 13671.543899999982, "isoelectric_point": 9.982042884826662 }, "id": "GLJLFKCOHC_399", "locus": "GCA013368035_00374" }, { "type": "cds", 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"score": 26.19732666015625, "start": 4, "stop": 105 }, { "id": "PF02737.24", "name": "3HCDH_N", "length": 75, "aa_cov": 0.5849056603773585, "hmm_cov": 0.9866666666666667, "evalue": 2.9466946028191683e-08, "score": 29.20623207092285, "start": 4, "stop": 65 }, { "id": "PF02558.23", "name": "ApbA", "length": 99, "aa_cov": 0.9150943396226415, "hmm_cov": 0.9797979797979798, "evalue": 1.8820941653309044e-09, "score": 32.73456573486328, "start": 4, "stop": 100 }, { "id": "PF03446.21", "name": "NAD_binding_2", "length": 104, "aa_cov": 0.9433962264150944, "hmm_cov": 0.8846153846153846, "evalue": 6.980856021010536e-10, "score": 34.553951263427734, "start": 4, "stop": 103 }, { "id": "PF02153.23", "name": "PDH_N", "length": 88, "aa_cov": 0.8301886792452831, "hmm_cov": 0.8977272727272727, "evalue": 1.207581597753556e-07, "score": 26.760461807250977, "start": 16, "stop": 103 }, { "id": "PF03721.20", "name": "UDPG_MGDP_dh_N", "length": 86, "aa_cov": 0.7358490566037735, "hmm_cov": 1.0, "evalue": 8.041695011760702e-08, "score": 27.44905662536621, "start": 3, "stop": 80 } ], "seq_stats": { "molecular_weight": 11723.46249999999, "isoelectric_point": 8.990712547302245 }, "id": "GLJLFKCOHC_400", "locus": "GCA013368035_00375" }, { "type": "cds", "sequence": "contig_1", "start": 212568, "stop": 213200, "strand": "+", "frame": 3, "gene": "gpsA", "product": "Glycerol-3-phosphate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0240", "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65", "UniRef:UniRef90_D1J7V6" ], "nt": "ATGACACAAGAAGCCCAAGCTAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGATATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGCGGGGGTAAGCTGAAAACGATTTTTGGATTGTCGTGCCTAGGTGATTTAATGCTAACAGCATTGTCAGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCGCTTTATCAAATAATATACAAGAATGCTAACCCTAATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAA", "aa": "MTQEAQANSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPNEIIINLMKRKLKSE", "aa_hexdigest": "ab49fe71c6c7049995428b1e6d484967", "start_type": "ATG", "rbs_motif": null, "genes": [ "gpsA" ], "truncated": "5-prime", "pseudogene": { "start": 212268, "stop": 213200, "observations": { "mutation": [ 212535 ], "stop": [ 212535 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V6", "query_cov": 1.0, "subject_cov": 0.6422018348623854, "identity": 0.995, "score": 399.0, "evalue": 1.95e-138, "gene_start": 1, "gene_end": 210, "reference_start": 118, "reference_end": 327, "reference_sequence": "MANISIFGSGAMGTAVATILANNGHNITIYGIDDSELNDLKNGRNTKYFEDVNLPKFKVTKDKKQAVEDCNYIVFAIPTKALPETYKEVIKLIKKQVIIINVSKGFWKDSNKSCHEIMTQEAQDNSLISGVVSLIGPSFAIDIINKNITAVDAVSNNDKLNKKVQKLFSNDYFRVYTQKDVAGAESGSIFKNILAIASGMVAGLGYSTNSQIALITRGFNELKKFVLARGGKLKTIFGLSCLGDLMLTALSDKSRNYTFGKNFYNKNFDSSKLTVEGLRSIEIVYDEYIKTKLLDLPIVNSLYQIIYKNANPNEIIINLMKRKLKSE" }, "paralog": false, "identity": 0.9935483870967742, "score": 600.0, "evalue": 2.43e-220, "description": "Internal stop codon at 212535. Nonsense mutation around 212535.", "psc": { "uniref90_id": "UniRef90_D1J7V6", "gene": "GpsA", "product": "Glycerol-3-phosphate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z5IR65", "cog_id": "COG0240", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V6", "UniRef:UniRef50_A0A2Z5IR65", "COG:COG0240", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z5IR65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z5IR65" ], "product": "Glycerol-3-phosphate dehydrogenase [NAD(P)+]" } }, "id": "GLJLFKCOHC_401", "locus": "GCA013368035_00376" }, { "type": "cds", "sequence": "contig_1", "start": 213215, "stop": 213496, "strand": "+", "frame": 2, "gene": "hupA", "product": "HU family DNA-binding protein", "db_xrefs": [ "COG:B", "COG:COG0776", "RefSeq:WP_020002655.1", "SO:0001217", "UniParc:UPI0002F8284C", "UniRef:UniRef100_UPI0002F8284C", "UniRef:UniRef50_N9UB80", "UniRef:UniRef90_A0A454C9F8" ], "nt": "ATGAACAAGAAAGAACTACTAAGTAAAACTAGCAATGAAACAGGATTTGCGCATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGATGAAATAGAAGCGGGAAGAGAAGTTACAATTTCAGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCACAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTCAAATTCTCAACAGCTTTTAAAAAACGTATTAACAGCAAAAATTAA", "aa": "MNKKELLSKTSNETGFAHVLIESAFDEIIRIIADEIEAGREVTISGFGNFSSKFIKGKEKVHNITKEIINVPDRLDPRFKFSTAFKKRINSKN", "aa_hexdigest": "c995b4f9d9cab48df52b1eddb90ad6cd", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0002F8284C", "ncbi_nrp_id": "WP_020002655.1", "uniref100_id": "UniRef100_UPI0002F8284C", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F8284C", "RefSeq:WP_020002655.1", "UniRef:UniRef100_UPI0002F8284C" ] }, "ips": { "uniref100_id": "UniRef100_UPI0002F8284C", "uniref90_id": "UniRef90_A0A454C9F8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9F8", "gene": "HupA", "product": "HU family DNA-binding protein", "uniref50_id": "UniRef50_N9UB80", "cog_id": "COG0776", "cog_category": "B" }, "pscc": { "uniref50_id": "UniRef50_N9UB80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9UB80" ], "product": "DNA-binding histone-like protein HU" }, "genes": [ "hupA" ], "id": "GLJLFKCOHC_402", "locus": "GCA013368035_00377" }, { "type": "cds", "sequence": "contig_1", "start": 213820, "stop": 214023, "strand": "-", "frame": 2, "gene": "recU", "product": "Holliday junction resolvase RecU", "db_xrefs": [ "COG:COG3331", "COG:R", "EC:3.1.21.10", "KEGG:K03700", "SO:0001217", "UniRef:UniRef50_A0A077L6J4", "UniRef:UniRef90_D1J7V7" ], "nt": "ATGAAAAATAAAAATAATAGAGGTATGCTTTTAGAATCTATAATAAATAACACTAACGAGTATTATTTTAAAAATGGAATTGCTTTAATTCATAAAAAAAATTTGGATATTAAATTTAAAAATGTCAAATTAGAAAAAAATAAATTACTTTTAAACAATGCAAGCATATTTTCAAAAAGCACTGTAGATTATTATGGAGTTTGA", "aa": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNNASIFSKSTVDYYGV", "aa_hexdigest": "210d5eac37e9fcd468753e0f4ecee41b", "start_type": "ATG", "rbs_motif": null, "genes": [ "recU" ], "truncated": "5-prime", "pseudogene": { "start": 213520, "stop": 214023, "observations": { "mutation": [ 213696, 213822 ], "stop": [ 213696, 213822 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V7", "query_cov": 1.0, "subject_cov": 0.40119760479041916, "identity": 0.97, "score": 126.0, "evalue": 1.38e-35, "gene_start": 1, "gene_end": 67, "reference_start": 1, "reference_end": 67, "reference_sequence": "MKNKNNRGMLLESIINNTNEYYFKNGIALIHKKNLDIKFKNVKLEKNKLLLNDASIYSKSTVDYYGVWKGRFLAFEVKSTEQKNFEKKNIKKHQIEYLNKVLLHQGIAFWIIYFKFQNEFLLIEHANFKKCLFEEKKSYICFEALKSLGKEIKLIFPGILDFLNILK" }, "paralog": false, "identity": 0.9640718562874252, "score": 322.0, "evalue": 1.78e-115, "description": "Internal stop codon at 213696, 213822. Nonsense mutation around 213696, 213822.", "psc": { "uniref90_id": "UniRef90_D1J7V7", "gene": "recU", "product": "Holliday junction resolvase RecU", "ec_ids": [ "3.1.21.10" ], "uniref50_id": "UniRef50_A0A077L6J4", "cog_id": "COG3331", "cog_category": "R", "kegg_orthology_id": "K03700", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V7", "EC:3.1.21.10", "UniRef:UniRef50_A0A077L6J4", "COG:COG3331", "COG:R", "KEGG:K03700" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6J4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6J4" ], "product": "Holliday junction resolvase RecU" } }, "id": "GLJLFKCOHC_403", "locus": "GCA013368035_00378" }, { "type": "cds", "sequence": "contig_1", "start": 214061, "stop": 214663, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF11074.14" ], "nt": "TTGAATTACAACTATAAAACAATTATGAAAGTAATAAATGATTTATATGATGAGAAAGCAGATTATTATGTCGTATATAACGACACATATGAAGCATCTAGAATAAAAGAAATGCAAGAAATTTTGGAATATTACAAAGATGATGGATTTATTAGCCAAAATGAATTTGACATTTTAAAAGAAAAATCTCAATTTATATTAAGCAAGATTATTGATATAGAAAATTTATTTTTTAGACCAGCAATGGATTGCAATATAAAAAGCTCTATTGTAAATTTAGGGCAAATAAAAAATGTCTACTCAATAAAAAAGATTGAACTTTTTGTAAATGAAAATAAATTAAATTTAAAACATAAAATTTTTCCATATCACGAACTTGATGTAAAAAATGGCGGAATGGCATTGCAAATAGCAACGCTAAGAGCTTTAAATAAAATAAGAGATAATGAATGGACGGTCAAAGAAGCTCAATTAAAAAGATATTGCCATAACGATGTTTTGGCAATGATTATGGCATTTGATTTGGTTGAATTTTTGTTACAATCGAAAGATAATTATTTCAAAAACTTCGAAACATATAAAGACTTAAAAGCGAAAAAGTAG", "aa": "MNYNYKTIMKVINDLYDEKADYYVVYNDTYEASRIKEMQEILEYYKDDGFISQNEFDILKEKSQFILSKIIDIENLFFRPAMDCNIKSSIVNLGQIKNVYSIKKIELFVNENKLNLKHKIFPYHELDVKNGGMALQIATLRALNKIRDNEWTVKEAQLKRYCHNDVLAMIMAFDLVEFLLQSKDNYFKNFETYKDLKAKK", "aa_hexdigest": "76f03932df327d8d8ee354398abd533a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF11074.14", "name": "DUF2779", "length": 72, "aa_cov": 0.36, "hmm_cov": 0.7777777777777778, "evalue": 7.470243233739624e-07, "score": 24.90161895751953, "start": 6, "stop": 77 } ], "seq_stats": { "molecular_weight": 23869.297000000013, "isoelectric_point": 6.839913368225097 }, "id": "GLJLFKCOHC_404", "locus": "GCA013368035_00379" }, { "type": "cds", "sequence": "contig_1", "start": 214745, "stop": 214996, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAATACATTATTTGTCAAAAAGAATTATTAAAATTAAAAGTTAAATCATTAACTGATAATTCAATTGAAACCAAAGAAATTTTAGATTTTTATGAAAACAATTTAATTTCAATTTCACCCGATATTTTAAACAAGGATAAATGCCCTGAATATTTATTATTAAAAGAATTTAATAAAAAAATAGTTTGGTTTGATTTCGAAGGCGTTACCTTGCCCGTTCCAATGATTAACCACTCTCTAGCATGA", "aa": "MPKYIICQKELLKLKVKSLTDNSIETKEILDFYENNLISISPDILNKDKCPEYLLLKEFNKKIVWFDFEGVTLPVPMINHSLA", "aa_hexdigest": "08babe17744da99458a79d042aa17692", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9715.369199999994, "isoelectric_point": 5.757642173767089 }, "id": "GLJLFKCOHC_405", "locus": "GCA013368035_00380" }, { "type": "cds", "sequence": "contig_1", "start": 215435, "stop": 216067, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATGATTTTAGTGAAGTTTATGAAAGCGACAGCCAAGAAGAAAATAGTTATGAAATGTTGGAATTTGCAAATGATGATATTGAAAACATTCTTGACAAAAATTTTAATCAATACTTTGAAAATAACTCTACTAAAAACAAAGACTTTTTAAAGCTAAACACTATTGAAAAAATAAAAAAAATTCAACATGATATGCAAAATGATTCAACACTGTTTGAAAAAATTAATTCAGATGGTAAACTAGAATTATTTAACAAAATATTGGATAATTTTTTTCAGTTTCAAATGGTTGATAGTGGAATAGCCCTTGTTGAGGAACAAATTACAAATTTTTTGACAAAAAATTCTCTAAATAAAGTAAATATAATTTCTAAAAAATCTTCAACCGATGAAAAAATTTTACAAACAAAAGAATTTTTAATGCAAAATTCACTAATAATAAATCCTGTTTTTGAATATAAAAAATGCATATCAAAGCCTTTTTACTACGATAGCAAAACTAAGGAAATGGGAAATATTGTATATTCATCAAAAACTAGCCTAAAGGATTTATTAAGAGCATATTATGATTATTCCGTTCTTTCATCCTCAGGGATCGAAATCAATAAAATTTTCATTGTGAAACCTTGA", "aa": "MNDFSEVYESDSQEENSYEMLEFANDDIENILDKNFNQYFENNSTKNKDFLKLNTIEKIKKIQHDMQNDSTLFEKINSDGKLELFNKILDNFFQFQMVDSGIALVEEQITNFLTKNSLNKVNIISKKSSTDEKILQTKEFLMQNSLIINPVFEYKKCISKPFYYDSKTKEMGNIVYSSKTSLKDLLRAYYDYSVLSSSGIEINKIFIVKP", "aa_hexdigest": "89097e285d18800e2f4439833a5071af", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24610.545700000024, "isoelectric_point": 4.945185661315917 }, "id": "GLJLFKCOHC_406", "locus": "GCA013368035_00381" }, { "type": "cds", "sequence": "contig_1", "start": 216525, "stop": 216995, "strand": "-", "frame": 3, "gene": null, "product": "MAGa7180 family putative nuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0", "UniRef:UniRef90_D1J7V9" ], "nt": "ATGGAAAAAAAAATAATAATTCCTAAAAGAACATTTTTTAACAATAAAAATTACACTTTGGATTATAAAAATAAGACTTTAATAGTAGATCCAAAAATCTTAAAATCTATGCAAGAAAAAAAACCTGGTACTTTTGGAGGATTTAAAAAAATGACCGGTTCGGCGTTGGGAGCTATAATGGGATTGAGCTCATTTGACAGCCAATTTGAAGCATTTTGTAATATATGTGGTTTTAGAAAACCAATATTGAACAGGAAATATGTTGATGCAGGTATTGCATTAGAGCCAAAAATAGTAAAAATTATTGAAAAAAAATTAAATTCCAAATTGCTTAGATATGAAGCCAATCAATATAATTATGATTATTTTAAAGATAACGAGCTATTTGGAGGGTTGCCTGATGGCTATAGTCCCGAAAGACATTTAATTATTGAAATTAAAACTGTCGGGGAAAAAAATTATGAAAATTGA", "aa": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYEN", "aa_hexdigest": "62555fefa421922b2ff76884a03c3221", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 216222, "stop": 216995, "observations": { "mutation": [ 216272, 216311, 216368, 216527 ], "stop": [ 216272, 216311, 216368, 216527 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7V9", "query_cov": 1.0, "subject_cov": 0.5531914893617021, "identity": 1.0, "score": 318.0, "evalue": 2.92e-108, "gene_start": 1, "gene_end": 156, "reference_start": 1, "reference_end": 156, "reference_sequence": "MEKKIIIPKRTFFNNKNYTLDYKNKTLIVDPKILKSMQEKKPGTFGGFKKMTGSALGAIMGLSSFDSQFEAFCNICGFRKPILNRKYVDAGIALEPKIVKIIEKKLNSKLLRYEANQYNYDYFKDNELFGGLPDGYSPERHLIIEIKTVGEKNYENWIKYGVNPGYIKQAQLYTYLIGATSFSIVACFLKEEDYLNPNNVDITKRIIKNWTYILNNEQVQDDINYCMQWYKKYTQIGISPKWNEQIDQDLLKFLECNNQEEWKNLYLYWVDQGKAVPEYEQA" }, "paralog": false, "identity": 0.980544747081712, "score": 509.0, "evalue": 3.44e-186, "description": "Internal stop codon at 216272, 216311, 216368, 216527. Nonsense mutation around 216272, 216311, 216368, 216527.", "psc": { "uniref90_id": "UniRef90_D1J7V9", "product": "MAGa7180 family putative nuclease", "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7V9", "UniRef:UniRef50_A0A077LBU0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077LBU0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077LBU0" ], "product": "YqaJ viral recombinase domain-containing protein" } }, "id": "GLJLFKCOHC_407", "locus": "GCA013368035_00382" }, { "type": "cds", "sequence": "contig_1", "start": 217230, "stop": 218027, "strand": "+", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W0", "UniRef:UniRef90_D1J7W0" ], "nt": "ATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTAGTAGCAGCAGGATGCAATGATCCAAAGAATAAAAAAAACCCAAACAATAATGATCAAAGTCAAGATGGCCAAATTGAATTTCAAGAAGGCTTAGGATTAAAAATTGCAAAAATTGCCAAAGATCAAGAAAATGTTAAAGTTGATGAATTTGTAAAAGAAGCAAAGAGCGCAAAAACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGAAGCAAAATATGAAGTTGCTGCCGGTACACACGGCCATGCCGATGAAGGACTAGTACACTTAGATATTATTCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAAAGGAAACTGTTGAAGACACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGACTTTAATTCTTTACTTGAAAAATTAAAGGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACGAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAA", "aa": "MKKYTKILLTLGAISTSIVAVPLVAAGCNDPKNKKNPNNNDQSQDGQIEFQEGLGLKIAKIAKDQENVKVDEFVKEAKSAKTLDQIKALFTKYKIAYDLSEIPDEAKYEVAAGTHGHADEGLVHLDIIQTVNEKQNNNRFEIKGFKKETVEDTVQLGGYKLSTKILDNKKDFSLQEIKKQIIDAQEKDFNSLLEKLKEFVNIEVDENNKTKQFEFNKEKIELISQSGQIVFKGISIIDISKKVNEVGYKTESKTDFIIQGLNKVE", "aa_hexdigest": "015ee2e1da171a63314d85de3f7fad69", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J7W0", "query_cov": 1.0, "subject_cov": 0.9814814814814815, "identity": 1.0, "score": 494.0, "evalue": 5.58e-176, "valid": true, "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7W0" }, "pscc": { "uniref50_id": "UniRef50_D1J7W0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W0" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_408", "locus": "GCA013368035_00383" }, { "type": "cds", "sequence": "contig_1", "start": 218262, "stop": 218510, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTGATGTTCCTAATGCTAATATTGCAAGAACTATTAAAGGTATTGTAGCTAAAGCACTAACAATTATATTTACGACAAAATCGGGTGCTTTATTAAATTTATATGCACTTCAAATTCCTAGTAAATTTCCAATAATCAAAGAAAAAATAAAAATAATTACAGTAAATAAAATTGTTCATTTAAATTGCATAAAATACAATGTAATTATTGACAAATTAGTTGCTGCAAGCTCTTTTGAATAA", "aa": "MKIDVPNANIARTIKGIVAKALTIIFTTKSGALLNLYALQIPSKFPIIKEKIKIITVNKIVHLNCIKYNVIIDKLVAASSFE", "aa_hexdigest": "ef81c67cbfcf3365cb6cd33d2b3d9155", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9049.900799999994, "isoelectric_point": 9.945682716369632 }, "id": "GLJLFKCOHC_409", "locus": "GCA013368035_00384" }, { "type": "cds", "sequence": "contig_1", "start": 218659, "stop": 219006, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGTATTTCGCTAGGAAATTCAAAAGCTAAATTATTTAAACAAAACATTATTAAAGATTTAGTTATTGCTGAATTACAAGTCATTTTACCTTTTTATATATTGCTTTTTAGCACTTCATTAATAATTGAGAGAATTTTTTCAATACCAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTGAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGA", "aa": "MFGISLGNSKAKLFKQNIIKDLVIAELQVILPFYILLFSTSLIIERIFSIPGQSIFISYVFTKAELNCIMFYFSFNFFALLIARFINEIILNYLNPAQAIEKRIRWIKRKRLDYE", "aa_hexdigest": "e6e9d179d903562870f79f242fe213fd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13581.094299999975, "isoelectric_point": 9.671111869812012 }, "id": "GLJLFKCOHC_410", "locus": "GCA013368035_00385" }, { "type": "cds", "sequence": "contig_1", "start": 218999, "stop": 219667, "strand": "+", "frame": 2, "gene": "dppC", "product": "Oligopeptide transport system permease protein", "db_xrefs": [ "COG:COG1173", "COG:EP", "SO:0001217", "UniRef:UniRef50_D1J7W2", "UniRef:UniRef90_D1J7W2" ], "nt": "ATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCAATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTTTTTAACAAAAAGAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTGCTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCTTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGCATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGCGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATCAATATTTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAACCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTTTAGCAGCATTTTATGTTTTTAATCGTTACAAATGA", "aa": "MNELDNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYK", "aa_hexdigest": "0d3fc454d370c2f736a1855d6765b6a1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "dppC" ], "truncated": "3-prime", "pseudogene": { "start": 218999, "stop": 219970, "observations": { "mutation": [ 219665, 219725, 219761, 219797 ], "stop": [ 219665, 219725, 219761, 219797 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W2", "query_cov": 1.0, "subject_cov": 0.556390977443609, "identity": 0.995, "score": 416.0, "evalue": 3.81e-144, "gene_start": 1, "gene_end": 222, "reference_start": 1, "reference_end": 222, "reference_sequence": "MNELNNKKELFQFAKKRQQIHNYPIFQSETKLFWKRFFNKKSNIVIFSFLLAFILLTLILLCFVKFSPIHSIDNKSDFVNNLPPYYSQIITRNFERGYQLDLIRSIENQESIRAAKLGIKPIFRILYDSARDSGGSLTVNTDIVALTYNPYDLIKAINLNQNSININILNKVLLGTTESGIDIYSRIFCAFALTTSIILLAIFINIFIGFSLAAFYVFNRYKWYGIVIDKIAVTLNAIPEIIWIFLLCIFLGTNWYGILIAFILISWISFYELSKNEILNLIQKEYIKAAKIIGLNNFQITYRHLFKNVLPSLIILIADRLAINILIVASLAFLDFLTAESNLNIGTIFKEALSSIKVNPQYLIIVAVYITIFCILLKLFSIGLSMTYDPKISKIINRI" }, "paralog": false, "identity": 0.9814241486068112, "score": 612.0, "evalue": 1.5e-223, "description": "Internal stop codon at 219665, 219725, 219761, 219797. Nonsense mutation around 219665, 219725, 219761, 219797.", "psc": { "uniref90_id": "UniRef90_D1J7W2", "gene": "DppC", "product": "Oligopeptide transport system permease protein", "uniref50_id": "UniRef50_D1J7W2", "cog_id": "COG1173", "cog_category": "EP", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W2", "UniRef:UniRef50_D1J7W2", "COG:COG1173", "COG:EP" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W2" ], "product": "Oligopeptide transport system permease protein" } }, "id": "GLJLFKCOHC_411", "locus": "GCA013368035_00386" }, { "type": "cds", "sequence": "contig_1", "start": 219658, "stop": 219786, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGCAACTAAAATACCATATCAATTTGTGCCTAAAAATATACACAACAAAAATATTCAAATTATTTCGGGTATTGCATTAAGTGTAACAGCAATTTTATCAATAACAATTCCATATCATTTGTAA", "aa": "MKATKIPYQFVPKNIHNKNIQIISGIALSVTAILSITIPYHL", "aa_hexdigest": "48b6091c9e9aebdc135720157be74f6e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4690.592899999999, "isoelectric_point": 10.000351905822754 }, "id": "GLJLFKCOHC_412", "locus": "GCA013368035_00387" }, { "type": "cds", "sequence": "contig_1", "start": 220286, "stop": 220816, "strand": "+", "frame": 2, "gene": "yqdH", "product": "Iron-containing alcohol dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG1979", "SO:0001217", "UniRef:UniRef50_H3NQ88", "UniRef:UniRef90_D1J7W3" ], "nt": "ATGTTGAATTTTATTTATAAAAACGATTGCAAAATCATATTTGGAAAAGATTCAATTCAAAATTTAGAAGAAGAATTGAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAATAATCAAGGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGCAATGTTATTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATCGGTGGCGGTTCGGTAATCGACACCGCCAAAGCAATAGCAGTTGGAGCGAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGCACATTACCTATCGGAGTTATTTCGACAATTGCATCTAGCGGTTCCGAATCATCAAATTGCTCAATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTCGCAATAATTGATCCTTGA", "aa": "MLNFIYKNDCKIIFGKDSIQNLEEELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVIPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDVDVWKFYTYEDKPNCTLPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDP", "aa_hexdigest": "91dfa9ef0025895e42fa1755ee6bda21", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "yqdH" ], "truncated": "3-prime", "pseudogene": { "start": 220286, "stop": 221119, "observations": { "mutation": [ 220814, 221072 ], "stop": [ 220814, 221072 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W3", "query_cov": 1.0056818181818181, "subject_cov": 0.45618556701030927, "identity": 0.977, "score": 340.0, "evalue": 4.87e-115, "gene_start": 1, "gene_end": 176, "reference_start": 1, "reference_end": 177, "reference_sequence": "MLNFIYKNDCKIIFGKDSIQNLEGELKSLKVKKILIVYSGKYIFELKIYDEIIKACKKLNIEFIENGNVVPNPRIELVRKLVLETKAQNVDFILAIGGGSVIDTAKAIAVGAKSDDVDVWKFYTYEDKPNCALPIGVISTIASSGSESSNCSILSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKLLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQDEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.9748201438848921, "score": 531.0, "evalue": 7.44e-193, "description": "Internal stop codon at 220814, 221072. Nonsense mutation around 220814, 221072.", "psc": { "uniref90_id": "UniRef90_D1J7W3", "gene": "YqdH", "product": "Iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H3NQ88", "cog_id": "COG1979", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W3", "UniRef:UniRef50_H3NQ88", "COG:COG1979", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_H3NQ88", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H3NQ88" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "GLJLFKCOHC_413", "locus": "GCA013368035_00388" }, { "type": "cds", "sequence": "contig_1", "start": 220835, "stop": 221074, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF25137.1" ], "nt": "ATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATTTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAACGCTAAATCATTAATGGAAGATCCAAACGATATGAATGCAAGGTCAGAAATATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGA", "aa": "MYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKSLMEDPNDMNARSEIFLISIFAHNNMLDSGRVAD", "aa_hexdigest": "696df5e3348cc2d47aa23cefeffdd362", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF25137.1", "name": "ADH_Fe_C", "length": 59, "aa_cov": 0.7468354430379747, "hmm_cov": 0.9830508474576272, "evalue": 4.9251990270090676e-08, "score": 28.272659301757812, "start": 10, "stop": 68 } ], "seq_stats": { "molecular_weight": 8971.124699999993, "isoelectric_point": 4.593579673767089 }, "id": "GLJLFKCOHC_414", "locus": "GCA013368035_00389" }, { "type": "cds", "sequence": "contig_1", "start": 221147, "stop": 221449, "strand": "+", "frame": 2, "gene": null, "product": "iron-containing alcohol dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3", "UniRef:UniRef90_UPI00215D79B7" ], "nt": "ATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTTGACAAGGGTTTGAGCCAAAAAGAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGACAGTCAAACCATTGGTCATTATAAACCCCTTTTATCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAA", "aa": "MVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQKEKANLLSKKLDEFYRTIKMRTRLSELGVSCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK", "aa_hexdigest": "afcc850b6697d36dafbe6bf3ec3b29bb", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 220847, "stop": 221449, "observations": { "mutation": [ 221072 ], "stop": [ 221072 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D79B7", "query_cov": 1.0, "subject_cov": 0.425531914893617, "identity": 0.98, "score": 186.0, "evalue": 8.69e-58, "gene_start": 1, "gene_end": 100, "reference_start": 136, "reference_end": 235, "reference_sequence": "MSNENHKLGIEYDFIIPKFAIIDPWYTKSLPMYQISCGISDISSHLIERYYTNIEHVDATDYMIEGLLKALMINAKSLMEDPNDMNARSEIFLISIFAHNNMLDSGRVADWASHRIEHELSNFYGITHGEGMAIVMVAYAKYMAIEKPKKLAQLAERVFNVDKGLSQEEKANLLSKKLDEFYRTIKMRTRLSELGVGCEKFREMALSATKNDSQTIGHYKPLLSKEIIEILKIAK" }, "paralog": false, "identity": 0.985, "score": 385.0, "evalue": 1.07e-138, "description": "Internal stop codon at 221072. Nonsense mutation around 221072.", "psc": { "uniref90_id": "UniRef90_UPI00215D79B7", "product": "iron-containing alcohol dehydrogenase", "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D79B7", "UniRef:UniRef50_H1B6A3" ] }, "pscc": { "uniref50_id": "UniRef50_H1B6A3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1B6A3" ], "product": "Alcohol dehydrogenase iron-type/glycerol dehydrogenase GldA domain-containing protein" } }, "id": "GLJLFKCOHC_415", "locus": "GCA013368035_00390" }, { "type": "cds", "sequence": "contig_1", "start": 221501, "stop": 222688, "strand": "-", "frame": 1, "gene": "tktA", "product": "Transketolase", "db_xrefs": [ "COG:COG0021", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J7W4", "UniRef:UniRef90_D1J7W4" ], "nt": "ATGAATTTCAAATACATTGAAGTGTCAAATGGTCAATGTCGCTCAATAATAAAAGCAATAAAAAAAGCACAAAAAATTAATCAACCTGTGTTTATTAAAATTTTAAGTCCTCAAAGCGAACAATTATTTAAAAAGAACTTTGATAATTCAATAACTCATGAAAAATTAGAAGAATTCAAAGACAAAACCTCTTTTCAAAAAACAAATTTGAGTGATTTGTATTCTGAGGTTGTTGAGTTTTATGAAAATAGATTGCAAAAAATAGAAAACAAAATAAAAACATTTAGTAATTCAAACAATTTGCAAATCTTTTTAAAAAACAATATTCGTGAAAATATTAACGATGTTGAAGAATTCAAAGAAAAAACATACGTTGAAAATGCTTTAAAAATAATAAATAATTTATGTGATAAATACAAAAATTTACTAGTATTATCAAATGATATTTCTTATTTTCAAAATATTAAATCGTTAAATGGGATTTTTGCAAGCAACAATCTTGGAAGAGGAATTTTCTTAGGTAATAGAGAATTTACAATGATTAATATGGCAATGGGATTAGGTTTGCATTCAAGCATAGAAACAATTGTCATTGTAGATATTAATTATTTAGGTTATTTATTGCCAACATTGCAAGTCTTAAAATATTCAAATGCAAAAATTCTTATACTATTAATCAATAATAGAAATAATTTTAGCCTAAGCAATGAGCTTAAAATATTATATTCATATGACCATATAAATTTATTGAGGCCTTGCGATTTCAATGAATTAAAAGGAGCATTTGAATTTCATTTTAATCACACAAAAAAGTCATCAATAATTTATTTAGAAAATAAAGAAATTAAAGAAATAAAGGATACTTCAAAAGTTGAATTTGAAGATGGCCCATATTACATTAGAAAAATTAGTCAAACAACAAATATTATAGCTGCAGGATCTGATCTTAGAATAGCAGAGATTATTGCTAGAAGGTTAAATAGTTCTATTATTTCAATATCAAATATTGAAAGCATTGAAAGCCTGAACTACGATAGAAATAATTCAATACTAATTGAATCTTATGAGGCATTATCTCATGAAAAAATTGCTAAATATAACATTGCATTAAATAAATTTTGCAACCTTGAAAAAAATGATGAAGAGTATATTGTAGAAAAAGTTTTAACTTCAATAGTAAAAAAATAA", "aa": "MNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYVENALKIINNLCDKYKNLLVLSNDISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAEIIARRLNSSIISISNIESIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK", "aa_hexdigest": "e0377670b18cfd8abced62030814b40e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tktA" ], "truncated": "3-prime", "pseudogene": { "start": 221501, "stop": 222988, "observations": { "mutation": [ 222859, 222973, 222985 ], "stop": [ 222859, 222973, 222985 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W4", "query_cov": 1.0, "subject_cov": 0.6605351170568562, "identity": 0.987, "score": 735.0, "evalue": 5.33e-264, "gene_start": 1, "gene_end": 395, "reference_start": 204, "reference_end": 598, "reference_sequence": "MKNKKIDQIAIDNIKINSLNMAFSWKYSDFSSIMSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLINNRNQNISYSNWTLDWIKSGEIFLGISGGFALGNTIAQNYLNRLDKNINHFTYWYVTERELQEGSTQEALAYAGTHNLRQLIVIYDANEIEYTVDSKSINPENIAKKVESMNFKYIEVSNGQCRSIIKAIKKAQKINQPVFIKILSPQSEQLFKKNFDNSITHEKLEEFKDKTSFQKTNLSDLYSEVVEFYENRLQKIENKIKTFSNSNNLQIFLKNNIRENINDVEEFKEKTYIENALKIINNLCDKYKNLLVLSNNISYFQNIKSLNGIFASNNLGRGIFLGNREFTMINMAMGLGLHSSIETIVIVDINYLGYLLPTLQVLKYSNAKILILLINNRNNFSLSNELKILYSYDHINLLRPCDFNELKGAFEFHFNHTKKSSIIYLENKEIKEIKDTSKVEFEDGPYYIRKISQTTNIIAAGSDLRIAELIARRLNSSIISISNIGNIESLNYDRNNSILIESYEALSHEKIAKYNIALNKFCNLEKNDEEYIVEKVLTSIVKK" }, "paralog": false, "identity": 0.9838383838383838, "score": 950.0, "evalue": 0.0, "description": "Internal stop codon at 222859, 222973, 222985. Nonsense mutation around 222859, 222973, 222985.", "psc": { "uniref90_id": "UniRef90_D1J7W4", "gene": "TktA", "product": "Transketolase", "uniref50_id": "UniRef50_D1J7W4", "cog_id": "COG0021", "cog_category": "G", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W4", "UniRef:UniRef50_D1J7W4", "COG:COG0021", "COG:G" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W4" ], "product": "Transketolase" } }, "id": "GLJLFKCOHC_416", "locus": "GCA013368035_00391" }, { "type": "cds", "sequence": "contig_1", "start": 222983, "stop": 223198, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTGGGGCAAAAATATTTCATGCTCTTTTTGGCTATTTTTTCAATATAAATAGAAAAAATAATTCTGATGTCAATAGGGATAGATTTGTTTTTTTTAATCCCAACATATTGCCACTTTTTTATGAAATTTTGAGATTATATAGTTTAATTAGTGAAAATGAATACCTTAACTTAATAAGCAATAGAAATCAAAACATTTCATATTCGAATTGA", "aa": "MSGAKIFHALFGYFFNINRKNNSDVNRDRFVFFNPNILPLFYEILRLYSLISENEYLNLISNRNQNISYSN", "aa_hexdigest": "bf272d45ac44cd0e2f7558d7c310d171", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8501.515799999992, "isoelectric_point": 9.220993614196775 }, "id": "GLJLFKCOHC_417", "locus": "GCA013368035_00392" }, { "type": "cds", "sequence": "contig_1", "start": 223287, "stop": 223571, "strand": "-", "frame": 3, "gene": null, "product": "MMB_0454 family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5", "UniRef:UniRef90_D1J7W5" ], "nt": "ATGAACTTTCGTTATTTTGTTGAACAAGATGCAATATATGAAGAATTAAACAATATTTTTAGTTCAAATAAAAATATAAAATTAATTGGAAAAATAGAAATTAAACCAAATCGCACTAAAACAAATGTTTTTATTAATTTAAGTTACAAATTAATAAACAAAACTAGTTTTGCATATGAAACAAAAAAACTAATATTTAAGATTGAACATAAAGTTTTTTCATTAATAAATGCCAAACCAAACAATATTAATTTAATATTCCAAGGTTATGAAGATGAAAAATAA", "aa": "MNFRYFVEQDAIYEELNNIFSSNKNIKLIGKIEIKPNRTKTNVFINLSYKLINKTSFAYETKKLIFKIEHKVFSLINAKPNNINLIFQGYEDEK", "aa_hexdigest": "6260e9e8055bd6afa1fc15267aea064e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7W5", "query_cov": 1.0, "subject_cov": 0.912621359223301, "identity": 1.0, "score": 187.0, "evalue": 3.63e-60, "valid": true, "product": "MMB_0454 family protein", "uniref50_id": "UniRef50_D1J7W5" }, "pscc": { "uniref50_id": "UniRef50_D1J7W5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W5" ] }, "genes": [], "id": "GLJLFKCOHC_418", "locus": "GCA013368035_00393" }, { "type": "cds", "sequence": "contig_1", "start": 223860, "stop": 224159, "strand": "-", "frame": 3, "gene": "efp", "product": "elongation factor P", "db_xrefs": [ "COG:COG0231", "COG:J", "GO:0003746", "GO:0005737", "GO:0006414", "KEGG:K02356", "SO:0001217", "UniRef:UniRef50_Q98Q55", "UniRef:UniRef90_D1J7W6" ], "nt": "ATGATAAATGTTAACGATTTAAAACCAGGAGTTACATTTAAATTAGAAGGCAATATTTATGTAGTGCTTGAAGCAGCGCATTCAAAACAAGGCCGCGGCCAAGCAAATGTTAAAGCAAAAGTTAAAGATCTAAGAACAGGTTCAACAACAATTAAGTCATTTACTGGTGGCGAAAAAGTTGAACCAGCAATGATTGAAAAATCAAATATGGATTATCTATACAATGCAGGAGACGTTGTAGTTTTAATGGATCAAGAAACTTTCGAACAAATTGAAATACCTTCGGAACATCTTAATTGA", "aa": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLN", "aa_hexdigest": "4dfa5bee95f1db1ddb640fba54f22fd7", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "efp" ], "truncated": "5-prime", "pseudogene": { "start": 223602, "stop": 224159, "observations": { "mutation": [ 223862 ], "stop": [ 223862 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W6", "query_cov": 1.0, "subject_cov": 0.5351351351351351, "identity": 1.0, "score": 196.0, "evalue": 1.38e-62, "gene_start": 1, "gene_end": 99, "reference_start": 1, "reference_end": 99, "reference_sequence": "MINVNDLKPGVTFKLEGNIYVVLEAAHSKQGRGQANVKAKVKDLRTGSTTIKSFTGGEKVEPAMIEKSNMDYLYNAGDVVVLMDQETFEQIEIPSEHLNWELNFLKEGQKVIVRKFQNEVLDIELPANVNLKVVEAPDAVKGNTAQAAQKKVTLETGYVVETPLFIKEGEIVTISTETGKYTGRA" }, "paralog": false, "identity": 0.9945945945945946, "score": 358.0, "evalue": 1.12e-128, "description": "Internal stop codon at 223862. Nonsense mutation around 223862.", "psc": { "uniref90_id": "UniRef90_D1J7W6", "gene": "efp", "product": "elongation factor P", "uniref50_id": "UniRef50_Q98Q55", "cog_id": "COG0231", "cog_category": "J", "kegg_orthology_id": "K02356", "go_ids": [ "GO:0003746", "GO:0005737", "GO:0006414" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W6", "UniRef:UniRef50_Q98Q55", "COG:COG0231", "COG:J", "KEGG:K02356", "GO:0003746", "GO:0005737", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q55", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q55" ], "product": "Elongation factor P" } }, "id": "GLJLFKCOHC_419", "locus": "GCA013368035_00394" }, { "type": "cds", "sequence": "contig_1", "start": 224231, "stop": 224491, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAACCATTTGGAAAAGCTAATTCAGAAATTAACGCTAATGTTGAACTATCAATTCCACCAGATATTCCTAATGTTATACCTTTAGAATTTGAGGAGTTTATTTTTTGCTTTAATCAAGAAACTATTTTATTGATTAAAACCGAATATATCTTCAATTCTTTTTTTGTCTTAGGAACTTTTCTTAATAAATCTTCCATTTTAACCCGCCTTATTTCAATTATCATTAAATTTAACAATAATAAATGTAAAATTCCATAA", "aa": "MQPFGKANSEINANVELSIPPDIPNVIPLEFEEFIFCFNQETILLIKTEYIFNSFFVLGTFLNKSSILTRLISIIIKFNNNKCKIP", "aa_hexdigest": "7d7aa03bb50d67b20e90454849000958", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9884.486799999984, "isoelectric_point": 5.258880043029785 }, "id": "GLJLFKCOHC_420", "locus": "GCA013368035_00395" }, { "type": "cds", "sequence": "contig_1", "start": 224547, "stop": 225056, "strand": "+", "frame": 3, "gene": null, "product": "NAD(+) synthase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD8376" ], "nt": "TTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAATTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAGACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATACGGTCTTGCATTTCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAAGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGATTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAAGGTCCAAAATTAAAATAA", "aa": "MQLKLKTKIEIINLSSCFKKIVKIANIIDQGSIANTKSRLFMTTLYGLAFQNKNLVLGTDNFDEYYLGYFTKYGDGGCDLLPFANIKKSDVYAMASLLGVPQEIIDKKPSATLYKNQYDEEELGFKYSDFEKYLLDPTSVSEELIKKIKNIHKQTEHKRKAIPKGPKLK", "aa_hexdigest": "6c82a6eb091e5d7e5e3d5a489affc069", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BD8376", "query_cov": 0.8757396449704142, "subject_cov": 1.0, "identity": 0.655, "score": 193.0, "evalue": 1.12e-60, "valid": false, "product": "NAD(+) synthase", "uniref50_id": "UniRef50_UPI0021BD8376" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD8376", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD8376" ], "product": "NAD(+) synthase" }, "genes": [], "id": "GLJLFKCOHC_421", "locus": "GCA013368035_00396" }, { "type": "cds", "sequence": "contig_1", "start": 225056, "stop": 225238, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCAATATAAAAATTCAAGCATCCTCACCTAAATGCTTGAATTTTTATATTGTCTTTTATGCTTTTAAATTGTTTGCTTTGGTTCAATTGCCAAAAAAATTATCTAAGTTTAATGTTTTTATTGTTGAAATGTTTAGTTTAATTAATCCTTTGTTTTCAATATTAAATAAGGGGTTGTAA", "aa": "MFNIKIQASSPKCLNFYIVFYAFKLFALVQLPKKLSKFNVFIVEMFSLINPLFSILNKGL", "aa_hexdigest": "70315eb750b0c13f24b5c3a8030fb695", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6978.457399999996, "isoelectric_point": 9.924085807800292 }, "id": "GLJLFKCOHC_422", "locus": "GCA013368035_00397" }, { "type": "cds", "sequence": "contig_1", "start": 225616, "stop": 226245, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53", "UniRef:UniRef90_UPI001E586E53" ], "nt": "TTGTACCAAGTAGTTGTTGAAAAAAAGGCAAATGATATCCTAGCAAAAAACTATATTTGTGAAGATGTAACTTTTAAAATATCTGCTTCAAAATCTTTAAAATCGAATACTTTAGTATCTAATACGATGCTAAAAAAATATCCAATATTTATCAAAATAAATAGAGATGAAAAAAGCAATAAATTATCAATCACTCTCTCAATTCATAGATTGATTTATGAAGTTGCTACAAAAAAAGAAATTTCAATAAATAAAAAATCTCTAAATTTGTTGATTTATCATAAAACAAATGATGAATATATTCACTTTAAAATTGATAAATACGAAGATGGAAAACCTATATTTTTAGAAAAAAAACTGATAATAAAAATTGATGCTTATACAAAAATTAGAGATTATTTGAGCTATAAAGTCGGAGCCAATATTCTTATTAACTATGACTTAGCAAATAACCTATCAGATAATAGAAATATAGACTCAGATTTTAAAACAAGAACCTTGAAAAATATTGATATTTTTCTTACTGACAAGAGTTTTAAAGAAGATGTTAATAATTCTTCTGCAACTGTAGTTAAAGAATTTTTTGTTACATATTTTAAATTCAATCAGCTGAAACAAAATATGCCTTGA", "aa": "MYQVVVEKKANDILAKNYICEDVTFKISASKSLKSNTLVSNTMLKKYPIFIKINRDEKSNKLSITLSIHRLIYEVATKKEISINKKSLNLLIYHKTNDEYIHFKIDKYEDGKPIFLEKKLIIKIDAYTKIRDYLSYKVGANILINYDLANNLSDNRNIDSDFKTRTLKNIDIFLTDKSFKEDVNNSSATVVKEFFVTYFKFNQLKQNMP", "aa_hexdigest": "fb8b1444b776484a6ca542f3bfc688f0", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001E586E53", "query_cov": 1.0, "subject_cov": 0.8293650793650794, "identity": 0.995, "score": 404.0, "evalue": 6.87e-142, "valid": true, "uniref50_id": "UniRef50_UPI001E586E53" }, "pscc": { "uniref50_id": "UniRef50_UPI001E586E53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E586E53" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24478.087500000016, "isoelectric_point": 9.438381004333493 }, "id": "GLJLFKCOHC_423", "locus": "GCA013368035_00398" }, { "type": "cds", "sequence": "contig_1", "start": 226599, "stop": 226862, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9", "UniRef:UniRef90_D1J7W9" ], "nt": "ATGCGTCGTAGTAGCAACAGAGCATTCTTTTTAAAATGTTTTAATTATTTTAAGGAAATAATAAAAGAGTTAAGGGAACGAAAAATTAAAATAGATATAAATAAAATTCCCGAAATTTTTAATAAAGAGAATTTAATTTCACTTAAATTTTTAATTCAAAAGAATGTTTTAAAAACAGCAAAATTGTTTGAATCATCATTGAGTGATGATTTGAAATGTATTTATATTAAATATTTTAAAGAACTTAAAGATGATATTAAGTGA", "aa": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKNVLKTAKLFESSLSDDLKCIYIKYFKELKDDIK", "aa_hexdigest": "5c29005e89a3f00d6fc57d259d7cb1ab", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 226515, "stop": 226862, "observations": { "mutation": [ 226532, 226601 ], "stop": [ 226532, 226601 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7W9", "query_cov": 1.0, "subject_cov": 0.7565217391304347, "identity": 1.0, "score": 169.0, "evalue": 5.84e-53, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MRRSSNRAFFLKCFNYFKEIIKELRERKIKIDINKIPEIFNKENLISLKFLIQKNVLKTAKLFESSLSDDLKCIYIKYFKELKDDIKWTDFFFSKSSYYRKLNYLILAIAWFLIF" }, "paralog": false, "identity": 0.9826086956521739, "score": 221.0, "evalue": 8.34e-76, "description": "Internal stop codon at 226532, 226601. Nonsense mutation around 226532, 226601.", "psc": { "uniref90_id": "UniRef90_D1J7W9", "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7W9", "UniRef:UniRef50_D1J7W9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7W9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7W9" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10597.509099999988, "isoelectric_point": 9.69677028656006 }, "id": "GLJLFKCOHC_424", "locus": "GCA013368035_00399" }, { "type": "cds", "sequence": "contig_1", "start": 227204, "stop": 227383, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "TTGGATGTATTAGTTTATTCTTCAGAAAGTTTTTTACCCGGAACATCATTAAATGCAAGACTAATTGGTGCAATGAAAATGATTGATGGTGGCGAAACAGATACTAAATTAATTGCAGTTCACGCAGATGATTATAGATTAGATAATATTAAATCATTAGCAGATGTCGATCAAATGTGA", "aa": "MDVLVYSSESFLPGTSLNARLIGAMKMIDGGETDTKLIAVHADDYRLDNIKSLADVDQM", "aa_hexdigest": "2761b4dabd5cd8b4bf0800825a52388c", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 58, "aa_cov": 0.9830508474576272, "hmm_cov": 1.0, "evalue": 1.404365312960747e-17, "score": 59.326881408691406, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 6446.274400000002, "isoelectric_point": 4.331608772277833 }, "id": "GLJLFKCOHC_425", "locus": "GCA013368035_00400" }, { "type": "cds", "sequence": "contig_1", "start": 227396, "stop": 227551, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00719.24" ], "nt": "ATGAAAAATTTAGAAGTAAACATTGAAATTAGTAAAGATTCAAAAATTAAATATGAATATGACCGCAAAAGCAACAAAATCAAAGTTGACAGAATTTTAAGAGGTGATTTTAAATATCCTGCAAATTATGGCTATTTATCTGATGCACTAGATTGA", "aa": "MKNLEVNIEISKDSKIKYEYDRKSNKIKVDRILRGDFKYPANYGYLSDALD", "aa_hexdigest": "cc1867663a18a93573bdd0ed9b325cc6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00719.24", "name": "Pyrophosphatase", "length": 44, "aa_cov": 0.8627450980392157, "hmm_cov": 1.0, "evalue": 2.5300355600004506e-13, "score": 45.507972717285156, "start": 7, "stop": 50 } ], "seq_stats": { "molecular_weight": 6058.826600000001, "isoelectric_point": 8.92521266937256 }, "id": "GLJLFKCOHC_426", "locus": "GCA013368035_00401" }, { "type": "cds", "sequence": "contig_1", "start": 227680, "stop": 227952, "strand": "+", "frame": 1, "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "db_xrefs": [ "COG:COG0632", "COG:L", "EC:3.6.4.12", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379", "SO:0001217", "UniRef:UniRef50_B3PMK3", "UniRef:UniRef90_D1J7X1" ], "nt": "ATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACCAACTATTTAATTTTAGATCATAATGGTGAGGGTGAATTGATATACGTGGCAGATATAAAAAGATTTAATAAGGATGATTTGAGAAAAATATTTATTTACGATGTGGTAAATGAATATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCCAAGACAGCAATTTCTCTTCTAAATTTAGGATGA", "aa": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLG", "aa_hexdigest": "6ab85d8a3108cf0df2cf20e20a0b18c2", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "ruvA" ], "truncated": "3-prime", "pseudogene": { "start": 227680, "stop": 228255, "observations": { "mutation": [ 227950 ], "stop": [ 227950 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X1", "query_cov": 1.0, "subject_cov": 0.4368932038834951, "identity": 1.0, "score": 183.0, "evalue": 2.72e-57, "gene_start": 1, "gene_end": 90, "reference_start": 1, "reference_end": 90, "reference_sequence": "MKIYLYGKIMHVNTNYLILDHNGEGELIYVADIKRFNKDDLRKIFIYDVVNEYKKTTYGFENFKELIIFEDLISLQGLGPKTAISLLNLGWENLIQLIANEDKDKLCEASYVSLRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ" }, "paralog": false, "identity": 0.9947643979057592, "score": 371.0, "evalue": 7.86e-134, "description": "Internal stop codon at 227950. Nonsense mutation around 227950.", "psc": { "uniref90_id": "UniRef90_D1J7X1", "gene": "ruvA", "product": "Holliday junction branch migration protein RuvA", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_B3PMK3", "cog_id": "COG0632", "cog_category": "L", "go_ids": [ "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X1", "EC:3.6.4.12", "UniRef:UniRef50_B3PMK3", "COG:COG0632", "COG:L", "GO:0003678", "GO:0006281", "GO:0006310", "GO:0009378", "GO:0009379" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK3" ], "product": "Holliday junction branch migration complex subunit RuvA" } }, "id": "GLJLFKCOHC_427", "locus": "GCA013368035_00402" }, { "type": "cds", "sequence": "contig_1", "start": 228019, "stop": 228300, "strand": "+", "frame": 1, "gene": null, "product": "helix-hairpin-helix domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BC92F", "UniRef:UniRef90_UPI00280BC92F" ], "nt": "TTGAGGATTGCAAACAATATAATTTTTGCCTATAAACAAAAATATGCAAAATTTTTATCAAAATTAAGCGATGAAGATTTACTTAAAATCAAGAGCAAAAACACAAGCGACTCTTTAAGAGAAAAATTTGAATATACAATGAGAATGCTCGGTTTTAAAAAACAACAAATTTCATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAG", "aa": "MRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ", "aa_hexdigest": "a60e4a530ff81dcefc0d465912bc3ff2", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 227875, "stop": 228300, "observations": { "mutation": [ 227950 ], "stop": [ 227950 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280BC92F", "query_cov": 1.0, "subject_cov": 0.6595744680851063, "identity": 0.9890000000000001, "score": 171.0, "evalue": 2.08e-53, "gene_start": 1, "gene_end": 93, "reference_start": 49, "reference_end": 141, "reference_sequence": "MIIFEDLISLQGLGPKTAISLLNLGWENLIQLIANEDKDKLCEASYVSLRIANNIIFAYKQKYAKFLSKLSDEDLLKIKSKNTSDSLREKFEYTMRMLGFKKQQISYALEKMSITENIEESVENAIKIIGMKQNESRVLDQ" }, "paralog": false, "identity": 0.9858156028368794, "score": 265.0, "evalue": 8.62e-93, "description": "Internal stop codon at 227950. Nonsense mutation around 227950.", "psc": { "uniref90_id": "UniRef90_UPI00280BC92F", "product": "helix-hairpin-helix domain-containing protein", "uniref50_id": "UniRef50_UPI00280BC92F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280BC92F", "UniRef:UniRef50_UPI00280BC92F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280BC92F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BC92F" ], "product": "helix-hairpin-helix domain-containing protein" } }, "id": "GLJLFKCOHC_428", "locus": "GCA013368035_00403" }, { "type": "cds", "sequence": "contig_1", "start": 228275, "stop": 229228, "strand": "+", "frame": 2, "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "db_xrefs": [ "COG:COG2255", "COG:L", "EC:3.6.4.12", "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378", "RefSeq:WP_012855462.1", "SO:0001217", "UniParc:UPI0001BC2154", "UniRef:UniRef100_D1J7X2", "UniRef:UniRef50_D1J7X2", "UniRef:UniRef90_D1J7X2" ], "nt": "ATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAGCAAATTGATCATTTACTATTTTATGGTCCGCCAGGTTTAGGAAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGCAATATTGTTTATGTTCAAGGCCCACTTATTGAAAAAAAGAGTGATGTATTGACTATTTTTTCTTCAATAAATGATAATGACATAATTTTTATTGATGAAATTCATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAAGGAACAATTGATGTTGCGTTGGGCGTTGATGGTGATAAAAAAATCATGAGAATGAAACTAAAAAAATTTTGTTTGATTGCTGCCACAACAAAAATCAATTTATTATCCAAACCATTAAAAGATCGTTTTGGCTATATAGGAAAATTAAGCGATTATAGCGACTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATAAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTAGAAACTGTTAAGAAAGCCTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTACATAAAGTATTTAATGACAAATTTTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAA", "aa": "MSQEFSINSFDEFVGQEKLISTIKIIIESAKVQKKQIDHLLFYGPPGLGKTTLAKIISKETKSNIVYVQGPLIEKKSDVLTIFSSINDNDIIFIDEIHGINKNIEELFYSALEEGTIDVALGVDGDKKIMRMKLKKFCLIAATTKINLLSKPLKDRFGYIGKLSDYSDLEIAKIIFNSATKNKIKIKNDAINFIAIHSRQTPRVANTLLKRVNDFAIYYKIDLIDLETVKKAFSYLGIYLYGLYSPQIEYLKTLHKVFNDKFSSLDAISSIIKDDKYTILNDIEPILLIYKLIEKSPRGRKITNNGIEYLKSQNIIT", "aa_hexdigest": "2222fa95e3b47663b8bdd442e2d1d476", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2154", "ncbi_nrp_id": "WP_012855462.1", "uniref100_id": "UniRef100_D1J7X2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2154", "RefSeq:WP_012855462.1", "UniRef:UniRef100_D1J7X2" ] }, "ips": { "uniref100_id": "UniRef100_D1J7X2", "uniref90_id": "UniRef90_D1J7X2" }, "psc": { "uniref90_id": "UniRef90_D1J7X2", "gene": "ruvB", "product": "Holliday junction branch migration DNA helicase RuvB", "ec_ids": [ "3.6.4.12" ], "uniref50_id": "UniRef50_D1J7X2", "cog_id": "COG2255", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005524", "GO:0006281", "GO:0006310", "GO:0009378" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X2" ], "product": "Holliday junction branch migration complex subunit RuvB" }, "genes": [ "ruvB" ], "id": "GLJLFKCOHC_429", "locus": "GCA013368035_00404" }, { "type": "cds", "sequence": "contig_1", "start": 229363, "stop": 230016, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACAATTTTGGGATCGATTTTTTATTTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAGACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGGGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGCGGTAATCCATTATTTTATAAAGGTGAATATGTTTCTAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTGAAAAATGGACCTCAAAACTACAATATTAGCGTAGAAAAAAATCCAGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACATGCTTGCAACAAATTTATCTTTACAACGTAATAGAAAAGCCTCGCGTGAAGAAATTATAAATAACCCAGGCAATAAAATTTACTTTTGA", "aa": "MTILGSIFYLFPQLKKQSNSNVTSAKIGLKIERENQKEDSYKKSYGLSDQEILNLTKNYIGNQNGVLSTSYDINLVSKNLISATSYQDKTDKDVNNLISNFVNKPYLTVTDGGGNPLFYKGEYVSRFYPNPDVPRTLKDFLKNGPQNYNISVEKNPATTYNKFGARQKIQVKLDEYGWDSFVQMINEYMLATNLSLQRNRKASREEIINNPGNKIYF", "aa_hexdigest": "9c06c55cc74bd61fd65f24f5264c884c", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24848.692500000005, "isoelectric_point": 9.300289726257322 }, "id": "GLJLFKCOHC_430", "locus": "GCA013368035_00405" }, { "type": "cds", "sequence": "contig_1", "start": 230122, "stop": 230619, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGAAATAAAAAAGGACAAAAAAGGGAATATCATTGATATTAAATATCCTGTTTTAAAATCAAAAGAAATTAATGCAAGAAAATATTTGATATCAGCAGTCAACCCCGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCATCATTTTATTTAGCGAACGAAAATTTAGAAGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACCTACAACTATTTTTTAACCGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTCTATTTTCGTTATTTGCAGTATTTTTGATTGCTAAATATCGTTTATATGGAATTATATCTTCAATATGCTTGGCATTTATGATATTTGTGTTCTTATCAATTATTACTTCATTTAACATATATATAACAGGAGCCGTGGCATTTACTTTCTTAGTTTCTACATTCTTAACATTTTTAATGATTTGA", "aa": "MPEIKKDKKGNIIDIKYPVLKSKEINARKYLISAVNPATSRILSTEKNESSFYLANENLEGLSDEYIAAAINYSYAPFKLTKTYNYFLTGSSTNQNRYLAVLAILFSLFAVFLIAKYRLYGIISSICLAFMIFVFLSIITSFNIYITGAVAFTFLVSTFLTFLMI", "aa_hexdigest": "cafe84cf6cedce8975634cc8b80207ea", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18680.715099999994, "isoelectric_point": 9.340195655822754 }, "id": "GLJLFKCOHC_431", "locus": "GCA013368035_00406" }, { "type": "cds", "sequence": "contig_1", "start": 230932, "stop": 231129, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGCAAAAATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTTTGGAATATATAGCGGTGTATTTGGAAATACAAAATTAGGAATTAATGCTAGTGAAACATTAGCGAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGCGATTGA", "aa": "MIAKIDLISKSKYFSIGFAIFVILTIVFFGIYSGVFGNTKLGINASETLANNYLYSIQKSSINGD", "aa_hexdigest": "5dc74939cf120b0251bb17f902cf3353", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7118.2035, "isoelectric_point": 9.100760078430177 }, "id": "GLJLFKCOHC_432", "locus": "GCA013368035_00407" }, { "type": "cds", "sequence": "contig_1", "start": 231147, "stop": 231545, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATTGTCCATGGCAAATTATTAATCAAACTATTGAACTTATAACTGCTAATATTTCGTTTATTCACATTACAAAAAACGCTATTAAACCAAATCTTATAATGCTATATATTCCACAAATTATTAATGAAACCGCAATTATTAGAATTGTAAATCCAAAATTGATTAATTCACCATTTGCATTTAATAATATTTCGTGTATGTTCAAATCAAATTCATTACCTATAGCTGAAATTTTAGTATTAATAAATGTCTTTAAAGCTTCTAATTTAGTTGAATCAATCTTGTCTGCTGAAGAAATTAATAAATTATATGAGTCGTTAGTTAAACTTGATTTTTCAAAGAAAATATTTGCATTTGAAATATTTAATTCTTTTCAATATTTAATAATTTGA", "aa": "MLNCPWQIINQTIELITANISFIHITKNAIKPNLIMLYIPQIINETAIIRIVNPKLINSPFAFNNISCMFKSNSLPIAEILVLINVFKASNLVESILSAEEINKLYESLVKLDFSKKIFAFEIFNSFQYLII", "aa_hexdigest": "54508fa6cf189bf48b5cc78439cf2f29", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15119.826799999984, "isoelectric_point": 7.747688865661622 }, "id": "GLJLFKCOHC_433", "locus": "GCA013368035_00408" }, { "type": "cds", "sequence": "contig_1", "start": 231562, "stop": 231867, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAATTGACCAAATAAACAATATATTTAAAAAATATGTAATAGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCATCTACATATACGTTGATGACAGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTGCCAAACATTTGA", "aa": "MILSTAFSVVDSISNSQTIKEEFGNNLNTKNYIFEIDQINNIFKKYVIESLNKNIFNIFFAIIFVASTYTLMTGFNATTILVTSMFIISNSFINLFILPNI", "aa_hexdigest": "8d141c75162ba245d38f68e1dcf8deab", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11509.228099999984, "isoelectric_point": 5.052213478088378 }, "id": "GLJLFKCOHC_434", "locus": "GCA013368035_00409" }, { "type": "cds", "sequence": "contig_1", "start": 231999, "stop": 233276, "strand": "+", "frame": 3, "gene": "hisS", "product": "histidine--tRNA ligase", "db_xrefs": [ "COG:COG0124", "COG:J", "EC:6.1.1.21", "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427", "KEGG:K01892", "RefSeq:WP_114119126.1", "SO:0001217", "UniParc:UPI000DED59F8", "UniRef:UniRef100_UPI000DED59F8", "UniRef:UniRef50_A0A3Q9VA72", "UniRef:UniRef90_D1J7X4" ], "nt": "ATGTTTCAAAGACTTAAAGGTACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTGATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAGGATAATGGCCAAAGAGACATTGCCTTAAGACCAGAAGGAACCGCTTCAACAATAAGAGCTTTTGTAGAAAACAAAATAAATAATTTAGAACCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGCCCTCAAAAAGGTCGCTATAGACAATTTATTCAGGGAGGAGTTGAATTAATTGAAAATTCAACTATTGAGAATAATTTTGAAATAATAAAATTGGCATATGATTTTTTAAAGTCGTTAAAAATTAGTAACATTACTTTAGAAATAAATAATTTGGGCTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAAGAATATTTTTCTAAATACAAAGAACAATTGAATGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTGAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCTCCAAAATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAAAAATTTACTAGATTTACTAGATGAATTTGAAATCAAATATGTAATAAATCCCTACTTAGTCAGGGGGCTAGATTACTACAATGATATTGTTTTTGAATTTGTTTCAAATTCAGACGCCCTAGGTTCGCAATGTACCGTTCTTGGGGGTGGAAGATATGATGGTATGATTAAAAGCTTTGGCGGGCCTGATATAGGCTCTATTGGGTTTGCCTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAATTTTAATGAAACTATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAAGGATTTTCAAGTAATTTTTCCAAGTCAAAGACAATCAATTAAGTCTCTGTTTAGACTTCAATATAAACTACAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAAATGAGTTGAAAATAAAATCAGTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAA", "aa": "MFQRLKGTRDIYGLEEKVLNLIENKFFKIIKNYNFKRICTPIIEDANLFIRSVGETSDIVSKEMYLFKDNGQRDIALRPEGTASTIRAFVENKINNLEPTKLAYFGPMFRYERPQKGRYRQFIQGGVELIENSTIENNFEIIKLAYDFLKSLKISNITLEINNLGSFESRNNYIEILKEYFSKYKEQLNEISLIRLQKNVLRILDDKEEIKKDFVKNAPKLIDYLSSIEKERFKNLLDLLDEFEIKYVINPYLVRGLDYYNDIVFEFVSNSDALGSQCTVLGGGRYDGMIKSFGGPDIGSIGFAFGVDRLMEIIMSNIDNYPELNFNETILIAYLNEDEKNEIRKIAYDLRKDFQVIFPSQRQSIKSLFRLQYKLQPKYLIFKELNMKKNELKIKSVLNERNIIYTNLDEFKNIISEMKRDENNE", "aa_hexdigest": "8caf75368a93538ae54fc34551aa3708", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000DED59F8", "ncbi_nrp_id": "WP_114119126.1", "uniref100_id": "UniRef100_UPI000DED59F8", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DED59F8", "RefSeq:WP_114119126.1", "UniRef:UniRef100_UPI000DED59F8" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DED59F8", "uniref90_id": "UniRef90_D1J7X4" }, "psc": { "uniref90_id": "UniRef90_D1J7X4", "gene": "hisS", "product": "histidine--tRNA ligase", "ec_ids": [ "6.1.1.21" ], "uniref50_id": "UniRef50_A0A3Q9VA72", "cog_id": "COG0124", "cog_category": "J", "kegg_orthology_id": "K01892", "go_ids": [ "GO:0004821", "GO:0005524", "GO:0005737", "GO:0006427" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3Q9VA72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VA72" ], "product": "Histidine--tRNA ligase" }, "genes": [ "hisS" ], "id": "GLJLFKCOHC_435", "locus": "GCA013368035_00410" }, { "type": "cds", "sequence": "contig_1", "start": 233401, "stop": 234012, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "GTGCAATTAATATTTCAAAATGTATCAGACCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAATAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTCGATAAACAAGGTTACATTGAAGTAGAAACTCCAATTTTAGGTAAATCAACTCCTGAGGGAGCGAGAGATTACTTGGTTCCTACTAGAAGAAAAGGAAGATTTTTTGCATTGCCCCAATCACCACAACTATTTAAACAATTATTGATGGCTGCCGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTCAGAGATGAAGATTTAAGAAAAGATAGACAACCAGAATTTACTCAATTAGATATTGAAATGTCATTTGGAAGTCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGA", "aa": "MQLIFQNVSDPLLTKESCIYVKGTVQKRLEVNPALKTGEIEVIVSDYKIFNSAKQIPFEINNSKSTNDANEDIRLEYRFLDLREERMLNNLRLRHKFLLLVRNFFDKQGYIEVETPILGKSTPEGARDYLVPTRRKGRFFALPQSPQLFKQLLMAAGFEKYFQIARVFRDEDLRKDRQPEFTQLDIEMSFGSQEEIFKLCEEM", "aa_hexdigest": "56df0164e538cf164b07c9a2d475a141", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_G9YIR7", "query_cov": 0.9310344827586207, "subject_cov": 0.8217391304347826, "identity": 0.556, "score": 193.0, "evalue": 7.66e-59, "valid": false, "gene": "aspS", "product": "aspartate--tRNA ligase", "ec_ids": [ "6.1.1.-" ], "uniref50_id": "UniRef50_A0A951N4R3", "go_ids": [ "GO:0004815", "GO:0005524", "GO:0005737", "GO:0006422" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 136, "aa_cov": 0.6600985221674877, "hmm_cov": 1.0, "evalue": 1.3030560186627193e-60, "score": 200.42628479003906, "start": 70, "stop": 203 } ], "seq_stats": { "molecular_weight": 23943.272099999984, "isoelectric_point": 6.65228786468506 }, "id": "GLJLFKCOHC_436", "locus": "GCA013368035_00411" }, { "type": "cds", "sequence": "contig_1", "start": 234031, "stop": 234351, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGGTACAAAATTGAAACTCCGTTTCCAAAAATGGACTTTTTCTATTCGATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATCGAAGATTATTCAAAACAATTAGCAACTAAATATAATAAAAAATTTGTAAAAGCTATTGTTTTTGACAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTGGATATATTTGATTTAAAAGAAGCAAACAAAAATGAATTCCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGA", "aa": "MGYKIETPFPKMDFFYSMAHYGNDKPDTRFGFLIEDYSKQLATKYNKKFVKAIVFDRNINEHKQIINETFKKNNGQILDIFDLKEANKNEFQCLLDKAKEDNISTP", "aa_hexdigest": "c70e994752e3ded1b67ee581a190c939", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12539.144999999984, "isoelectric_point": 7.782381629943847 }, "id": "GLJLFKCOHC_437", "locus": "GCA013368035_00412" }, { "type": "cds", "sequence": "contig_1", "start": 234484, "stop": 234954, "strand": "+", "frame": 1, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "nt": "ATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATACGATTTAGTGTTAAACGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTCGGATTCTTTTTAAAAGCATTTGAATATGGTCTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATTAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGTATTGACTTGTTGACATCAGCTCCTAGCGATGTTACAAAAGAGCAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAA", "aa": "MFEYDRETKTYSPAHHAFTQFEESTMKYLETGELEKVRAKSYDLVLNGFELGSGSIRIYDPKIQKKMFDVINLSEKQQQDRFGFFLKAFEYGLPPHNGIAFGVERILMILTKSESIRDVIAFPKNAKGIDLLTSAPSDVTKEQLDEYGLELKINKK", "aa_hexdigest": "f007a3977b2685b4435b15c340846545", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021FB0CEA", "query_cov": 0.9743589743589743, "subject_cov": 0.8837209302325582, "identity": 0.5660000000000001, "score": 176.0, "evalue": 1.44e-53, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_UPI0021FB0CEA" }, "pscc": { "uniref50_id": "UniRef50_UPI0021FB0CEA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021FB0CEA" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "GLJLFKCOHC_438", "locus": "GCA013368035_00413" }, { "type": "cds", "sequence": "contig_1", "start": 235046, "stop": 235225, "strand": "+", "frame": 2, "gene": "secG", "product": "preprotein translocase subunit SecG", "db_xrefs": [ "GO:0015136", "GO:0015739", "GO:0016020", "SO:0001217", "UniRef:UniRef50_I5D5Q9", "UniRef:UniRef90_D1J7X6" ], "nt": "ATGCATGCTGCTTCAATAGCCTTAACAGTTATATTGGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGCGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATTAAAAAAGTATTAAAGTGA", "aa": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLK", "aa_hexdigest": "287b3e133ec6143731f4c8470f1a19f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secG" ], "truncated": "3-prime", "pseudogene": { "start": 235046, "stop": 235297, "observations": { "mutation": [ 235223 ], "stop": [ 235223 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X6", "query_cov": 1.0, "subject_cov": 0.7108433734939759, "identity": 1.0, "score": 105.0, "evalue": 2.03e-28, "gene_start": 1, "gene_end": 59, "reference_start": 1, "reference_end": 59, "reference_sequence": "MHAASIALTVILVIISFAIIGISFIMAPDSNSFSGALVGSNDLDLFKVSKERGIKKVLKWSMITLGILLFIFLIALRVVIQNG" }, "paralog": false, "identity": 0.9879518072289156, "score": 151.0, "evalue": 3e-49, "description": "Internal stop codon at 235223. Nonsense mutation around 235223.", "psc": { "uniref90_id": "UniRef90_D1J7X6", "gene": "secG", "product": "preprotein translocase subunit SecG", "uniref50_id": "UniRef50_I5D5Q9", "go_ids": [ "GO:0015136", "GO:0015739", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X6", "UniRef:UniRef50_I5D5Q9", "GO:0015136", "GO:0015739", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_I5D5Q9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_I5D5Q9" ], "product": "Protein-export membrane protein SecG" } }, "id": "GLJLFKCOHC_439", "locus": "GCA013368035_00414" }, { "type": "cds", "sequence": "contig_1", "start": 235313, "stop": 236203, "strand": "+", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "ATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCCAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTATTTAGTGCCTTTCTTGCGCCTTTTCAATTAAAAGCGGTATTGGACAAAGATTTATTGCTTGCAAATATTTATTATTATTATGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGCAAGAAAAACTATTGTTGGAACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGTGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAGAATAATTGA", "aa": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNN", "aa_hexdigest": "b84779a2db2508ed1bd633966b9eb56a", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnr" ], "truncated": "3-prime", "pseudogene": { "start": 235313, "stop": 236506, "observations": { "mutation": [ 236201 ], "stop": [ 236201 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.41283124128312415, "identity": 1.0, "score": 570.0, "evalue": 5.8e-199, "gene_start": 1, "gene_end": 296, "reference_start": 1, "reference_end": 296, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9974811083123426, "score": 782.0, "evalue": 1.57e-284, "description": "Internal stop codon at 236201. Nonsense mutation around 236201.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "GLJLFKCOHC_440", "locus": "GCA013368035_00415" }, { "type": "cds", "sequence": "contig_1", "start": 236276, "stop": 237466, "strand": "+", "frame": 2, "gene": "rnr", "product": "ribonuclease R", "db_xrefs": [ "COG:COG0557", "COG:K", "EC:3.1.13.1", "GO:0003723", "GO:0004518", "KEGG:K12573", "SO:0001217", "UniRef:UniRef50_B3PMK9", "UniRef:UniRef90_D1J7X7" ], "nt": "TTGCGTGGCACAAGCATTTACTTACCCGATAGGGTTATACCAATGTTGCCGGTTGAATTGTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAACAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTGAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAACGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATTGGCCATTTCGGTTTAGCTTCAAAAGCTTACTCACATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTAATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCCGATTTATCAGAATCGCTGAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGCGAATCTGAAAAAAGTGCAATGGTTGTCGAACGAAGTGTTGTTGATGTTAGAAAATCAGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAACATTGACGAATATCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTCACGATTCCAACTAATAAAGACAACGATTTTCAAGCATCATCTAAAAATAAAATCTATAAAGCAGGCAATAAGTATTTAATTACTATCACTTCGATAGATCACGAAAAAGGAAATGTAAATGCAACATTAGCTTAG", "aa": "MRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSESLKQKISQIALMNSESEKSAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLTIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA", "aa_hexdigest": "2ac877ead2a16f65d2379c983d5a7f5c", "start_type": "TTG", "rbs_motif": "GGAG/GAGG", "genes": [ "rnr" ], "truncated": "5-prime", "pseudogene": { "start": 235976, "stop": 237466, "observations": { "mutation": [ 236201 ], "stop": [ 236201 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7X7", "query_cov": 1.0, "subject_cov": 0.5523012552301255, "identity": 0.99, "score": 762.0, "evalue": 8.63e-273, "gene_start": 1, "gene_end": 396, "reference_start": 322, "reference_end": 717, "reference_sequence": "MTYIKNFKNKNNNPKINEEQVLKCIRESDGITFIEIAKKLRIPAFLNFELSEILNNYLSKKLIDTKSDEKYIAIYFLTKVQKEISITAKRLGFIDFEMQEESQTKVFSAFLAPFQLKAVLDKDLLLANIYYYYENGIKKYKGDIIQILNHARKTIVGTIEKSANKLFFNAYDEKDRAKFEIINEKECPDITKLKNSLVSCEILKPNNKKVAIAYKNIIGNINDKEHVIKKIIAQNDVNVNFPQNLLEYVSSIPSFVSQEEINSRINLIDLMTVTIDGLDTKDFDDAISCYKNEKNNWKLFIHIADVSYYVKENDLIDNEALLRGTSIYLPDRVIPMLPVELSNGICSLNPNEVRACITLELEIDKQGNNISYKIYPSVIKSNYRLTYNEVNDFYSNIKSVPDDVANMLLHARDLAKIIRSKKISEGYVDFEIQESKVIMDGTKVVDIKVKEEGESEKLIEDFMVRANETVAQMMTNLKIPSIYRIHEKPSPEKLTLLQELFNFCGFKDVKVPYDGEPKSFGEMIAKLKEHKFDDFIKMALLRTMQKAIYSSNNIGHFGLASKAYSHFTSPIRRYPDLLLHRLIRTYIFEKKEITPDLSETLKQKISQIALMNSESEKNAMVVERSVVDVRKSEFFENLINKQFFATLVSIEKFGMFFNIDEYQTSVLVRFENLAIPTNKDNDFQASSKNKIYKAGNKYLITITSIDHEKGNVNATLA" }, "paralog": false, "identity": 0.9919354838709677, "score": 967.0, "evalue": 0.0, "description": "Internal stop codon at 236201. Nonsense mutation around 236201.", "psc": { "uniref90_id": "UniRef90_D1J7X7", "gene": "rnr", "product": "ribonuclease R", "ec_ids": [ "3.1.13.1" ], "uniref50_id": "UniRef50_B3PMK9", "cog_id": "COG0557", "cog_category": "K", "kegg_orthology_id": "K12573", "go_ids": [ "GO:0003723", "GO:0004518" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7X7", "EC:3.1.13.1", "UniRef:UniRef50_B3PMK9", "COG:COG0557", "COG:K", "KEGG:K12573", "GO:0003723", "GO:0004518" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMK9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMK9" ], "product": "Ribonuclease R" } }, "id": "GLJLFKCOHC_441", "locus": "GCA013368035_00416" }, { "type": "tmRNA", "sequence": "contig_1", "start": 237967, "stop": 238337, "strand": "+", "gene": "ssrA", "product": "transfer-messenger RNA, SsrA", "db_xrefs": [ "SO:0000584" ], "tag": { "start": 238050, "stop": 238127, "aa": "AEEKQNKQSFVLNQMMSSNPVFAY", "nt": "GCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAA" }, "nt": "GGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAAACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCTAATGAATAGCTCGGTTCATTGCTAGCCTTTGGAGAGCTCAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGATTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAATGAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCA", "id": "GLJLFKCOHC_34", "locus": "GCA013368035_00417" }, { "type": "cds", "sequence": "contig_1", "start": 238546, "stop": 238728, "strand": "+", "frame": 1, "gene": null, "product": "DUF2188 domain-containing protein", "db_xrefs": [ "RefSeq:WP_020002640.1", "SO:0001217", "UniParc:UPI0002F57F90", "UniRef:UniRef100_A0A2K9YST0", "UniRef:UniRef50_B3PMJ9", "UniRef:UniRef90_A0A2K9YST0" ], "nt": "ATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGGGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAA", "aa": "MAVRLVVKHEDGWAVKNPKGKRALRVFKTQKEAVQYAKTLQDTSSVNVQSKAGKFRKFTS", "aa_hexdigest": "9af3d45b90630dd907ce1f2a199efb17", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0002F57F90", "ncbi_nrp_id": "WP_020002640.1", "uniref100_id": "UniRef100_A0A2K9YST0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0002F57F90", "RefSeq:WP_020002640.1", "UniRef:UniRef100_A0A2K9YST0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YST0", "uniref90_id": "UniRef90_A0A2K9YST0" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YST0", "product": "DUF2188 domain-containing protein", "uniref50_id": "UniRef50_B3PMJ9" }, "pscc": { "uniref50_id": "UniRef50_B3PMJ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMJ9" ], "product": "DUF2188 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_442", "locus": "GCA013368035_00418" }, { "type": "cds", "sequence": "contig_1", "start": 238685, "stop": 239608, "strand": "+", "frame": 2, "gene": "folD", "product": "Bifunctional protein FolD", "db_xrefs": [ "COG:COG0190", "COG:H", "SO:0001217", "UniRef:UniRef50_D1J7X9", "UniRef:UniRef90_D1J7X9" ], "nt": "ATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAAAAGTTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATTTCTGAAGCGATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTACTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGTAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCGCAAAAGATCCAATTACATACGACCAAGTAAAGCCTGGGGCGGTTATTATTGATGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAATGAATTTTCTAATGTTGCCTCATTTATAACCCCTGTTCCCGGAGGGGTTGGTCCAATGACTATTTCGGCATTGCTCATTAATCTTTTAAAAGCAAGTTCCTTGCAAAATCCAATTATTGTACCATATCTTAAATTTTTAAAATCATATTTTAATTAA", "aa": "MFSLKLGSLESSQAKMAILLNGKAISLKVKEDLKKAIEKMPQEIRPTLGILQVGDLSESNIYVKNKLKYASDIGLNTKLIKLQQNASKLQISEAIELLNKQSTGFIVQIPMQTNQIIDSQEILDLISIDHDIDGLTSFNLDLKNPNNYFLSATASGIFLLLNKYNILYKNAKIGVIGQSKIVGYPLANFLVLKGYNVFRYNKDTPKNNIKDLDIIIVATGAKDPITYDQVKPGAVIIDVGIHRDSNNKISGDLNFNEFSNVASFITPVPGGVGPMTISALLINLLKASSLQNPIIVPYLKFLKSYFN", "aa_hexdigest": "92847b4de048a7a790c6d1545fd6844e", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J7X9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.993, "score": 580.0, "evalue": 6.03e-209, "valid": true, "gene": "FolD", "product": "Bifunctional protein FolD", "uniref50_id": "UniRef50_D1J7X9", "cog_id": "COG0190", "cog_category": "H" }, "pscc": { "uniref50_id": "UniRef50_D1J7X9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7X9" ], "product": "Bifunctional protein FolD" }, "genes": [ "folD" ], "id": "GLJLFKCOHC_443", "locus": "GCA013368035_00419" }, { "type": "cds", "sequence": "contig_1", "start": 239707, "stop": 239844, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATAGGTGTTAGTGGCATGATTGCTGCCGGTAAAAGTTCATTAGCTGAAAAACTTCATCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGACGACGAAGTATTTAATACATTTTTGAAATGA", "aa": "MIIGVSGMIAAGKSSLAEKLHRYFEGSLMLHEFEEDDEVFNTFLK", "aa_hexdigest": "0bfc1de864416f9cb21613380c6e5897", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5091.789099999999, "isoelectric_point": 4.8198556900024405 }, "id": "GLJLFKCOHC_444", "locus": "GCA013368035_00420" }, { "type": "cds", "sequence": "contig_1", "start": 239968, "stop": 240186, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01712.25" ], "nt": "ATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTAATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATATTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGGCAAAGTGAAGTAGATAACTGA", "aa": "MIFLDRFSIEHYIFAKLILSKKGKRYLDAYDALFEKLISKDELPDFAIFLDINFETFKKRIFTRGRQSEVDN", "aa_hexdigest": "579e8b1a95c98a26c99315edf116751f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01712.25", "name": "dNK", "length": 62, "aa_cov": 0.8333333333333334, "hmm_cov": 1.0, "evalue": 1.0118120090145912e-07, "score": 27.327556610107422, "start": 10, "stop": 69 } ], "seq_stats": { "molecular_weight": 8669.956499999993, "isoelectric_point": 7.985392570495606 }, "id": "GLJLFKCOHC_445", "locus": "GCA013368035_00421" }, { "type": "cds", "sequence": "contig_1", "start": 240507, "stop": 240845, "strand": "+", "frame": 3, "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0634", "COG:F", "EC:2.4.2.8", "GO:0006166", "KEGG:K00760", "SO:0001217", "UniRef:UniRef50_L0RW64", "UniRef:UniRef90_D1J7Y1" ], "nt": "TTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTGGATTGCATGATAACTAGCTCATATGATGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGACTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCACTGCTAAAATCAAGAAATCCTAAATCATTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTAGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAGATGACTTTGTTGTTGGCTTTGGCTTCGATTGA", "aa": "MAQLIKDVTVDFVLDCMITSSYDGKDRSSGNVKITLDLQNNIADKDVLIVEDIVDSARTMTKVVSLLKSRNPKSLKVLTLLDKPSGRLVEFKQDNFGFEIGKDDFVVGFGFD", "aa_hexdigest": "7ffa06ac28e5651618ac95176dd3d0e6", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [ "hpt" ], "truncated": "both", "pseudogene": { "start": 240354, "stop": 240920, "observations": { "mutation": [ 240435, 240843 ], "stop": [ 240435, 240843 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y1", "query_cov": 1.0, "subject_cov": 0.5957446808510638, "identity": 0.982, "score": 213.0, "evalue": 1.05e-68, "gene_start": 1, "gene_end": 112, "reference_start": 52, "reference_end": 163, "reference_sequence": "MSQIDYRIKKVLFDQATLEKRIKELANWVNETYKDSKNLIIVGLLKGCLPFLAQLIKDVTVDFVLDCMITSSYDGKDRSSGNVKITLDLQNNIADKDVLIVEDIVDSARTMTKVVSLLKSRNPKSLKVLTLLDKPSGRLVEFKQDNFGFEIGKNDFVVGFGFDWEEKMRQLPYIGLLTDKEINKHKKH" }, "paralog": false, "identity": 0.9787234042553191, "score": 358.0, "evalue": 1e-128, "description": "Internal stop codon at 240435, 240843. Nonsense mutation around 240435, 240843.", "psc": { "uniref90_id": "UniRef90_D1J7Y1", "gene": "hpt", "product": "hypoxanthine phosphoribosyltransferase", "ec_ids": [ "2.4.2.8" ], "uniref50_id": "UniRef50_L0RW64", "cog_id": "COG0634", "cog_category": "F", "kegg_orthology_id": "K00760", "go_ids": [ "GO:0006166" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y1", "EC:2.4.2.8", "UniRef:UniRef50_L0RW64", "COG:COG0634", "COG:F", "KEGG:K00760", "GO:0006166" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW64", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW64" ], "product": "Hypoxanthine phosphoribosyltransferase" } }, "id": "GLJLFKCOHC_446", "locus": "GCA013368035_00422" }, { "type": "cds", "sequence": "contig_1", "start": 240931, "stop": 241287, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2", "UniRef:UniRef90_D1J7Y2" ], "nt": "ATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGTATACCTTTAGCTATTGGCTCTGCGTTTGTAGCATTTATTTTAATAGTTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAAACAAAAACAAAAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAACTAAATTCCTTAAAAATTGAAAAACAAATTGCTTATTTATTTGACCCACAAAAATTTTTTAAAGAAAATTTAAATAATTTATGA", "aa": "MILKLQNWQITETSNQDSGTSIPLAIGSAFVAFILIVAVVIFIIKKIINKQTKTKDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDPQKFFKENLNNL", "aa_hexdigest": "d00c65722b78724766f6a2bbc38bacdd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 240874, "stop": 241590, "observations": { "mutation": [ 240883, 241285, 241405 ], "stop": [ 240883, 241285, 241405 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y2", "query_cov": 1.0, "subject_cov": 0.4229390681003584, "identity": 0.9490000000000001, "score": 212.0, "evalue": 3.37e-67, "gene_start": 1, "gene_end": 118, "reference_start": 20, "reference_end": 137, "reference_sequence": "MVYWQIKKLINTKNTNNFSIILKLQNWQITETSNQDSSTSITLAIGSAFVAFILIAAVVIFIIKKIINKQSKTRDNSTNKELNKDNKNDEYNKKITLPFFNSEDLKRIDKLNSLKIEKQIAYLFDPQKFFKENLNNLWVKKYYEIIKKFKLEKSPTSKFNKLKINKYYKNYLNEVGQWNYCRHHIDEIKISGAIYSAHPQTKEYYKTGDVILVTYEQHLFLHYLIVMAKTTMPNHGMIVGLKFHFNDTKKCIEYWDKVIQKMCEKYLVKYDKNWSENLT" }, "paralog": false, "identity": 0.957983193277311, "score": 456.0, "evalue": 1.03e-165, "description": "Internal stop codon at 240883, 241285, 241405. Nonsense mutation around 240883, 241285, 241405.", "psc": { "uniref90_id": "UniRef90_D1J7Y2", "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y2", "UniRef:UniRef50_D1J7Y2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 13650.665499999976, "isoelectric_point": 9.426132011413575 }, "id": "GLJLFKCOHC_447", "locus": "GCA013368035_00423" }, { "type": "cds", "sequence": "contig_1", "start": 242431, "stop": 243228, "strand": "+", "frame": 1, "gene": "ecfA2", "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "COG:COG1122", "COG:PR", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "RefSeq:WP_012855474.1", "SO:0001217", "UniParc:UPI0001BC2196", "UniRef:UniRef100_D1J7Y4", "UniRef:UniRef50_Q98QH5", "UniRef:UniRef90_D1J7Y4" ], "nt": "ATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGTTCATCGAAGCTGGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGTCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACAGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAGGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTTGATGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATAAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGCGGTCATGTTATTGCTCAAGGTAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAACCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAA", "aa": "MIEIKKISYKYPGSSKLALENIDLKIEEGQYIAILGHNGSGKSTFSKLLSAIYKPTSGKIIIDGLEINSENLLEIRKKIGIVFQNPDNQFIGSTVEDDIAFGLENKMLKPEEMKTIIYKYAQEVGMLDQLDREPQNLSGGQKQRVAIASTLALDPKIIIFDEITSMLDPKGRNDIYKIIHNLHKKTNKTLISITHDMDEALLADKLIVFAGGHVIAQGKPIEILNNKEIIEIAKIDSPFIYKLSSLLNDIEPTYNREELIKEICK", "aa_hexdigest": "5a3ed23426316973a47bd29793f9d158", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC2196", "ncbi_nrp_id": "WP_012855474.1", "uniref100_id": "UniRef100_D1J7Y4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2196", "RefSeq:WP_012855474.1", "UniRef:UniRef100_D1J7Y4" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Y4", "uniref90_id": "UniRef90_D1J7Y4" }, "psc": { "uniref90_id": "UniRef90_D1J7Y4", "gene": "EcfA2", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q98QH5", "cog_id": "COG1122", "cog_category": "PR", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QH5" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA1" }, "genes": [ "ecfA2" ], "id": "GLJLFKCOHC_448", "locus": "GCA013368035_00424" }, { "type": "cds", "sequence": "contig_1", "start": 243219, "stop": 243518, "strand": "+", "frame": 3, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6", "UniRef:UniRef90_UPI001E584429" ], "nt": "ATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCCATTATTGGCCCAACGGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTACAACTGGAAAAATTATCTTTAATGAGCTGCCAATAAAAATTAAAACTCCGAAACCTAGAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGA", "aa": "MQIKASEITKEYDKGLPTYIKVIDNVSLEISEGEAISIIGPTGSGKTTLIEHFNGLIIPTTGKIIFNELPIKIKTPKPRKPNLKKIKHDSNEYEIVLEK", "aa_hexdigest": "9388fc2cb7bf7b967813e783233e2e60", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_UPI001E584429", "query_cov": 0.8484848484848485, "subject_cov": 0.9545454545454546, "identity": 0.976, "score": 164.0, "evalue": 4e-51, "valid": true, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A448ZZL6" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZZL6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZZL6" ], "product": "Cobalt ABC transporter ATP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_449", "locus": "GCA013368035_00425" }, { "type": "cds", "sequence": "contig_1", "start": 243642, "stop": 244016, "strand": "+", "frame": 3, "gene": null, "product": "energy-coupling factor transporter ATPase", "db_xrefs": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626", "SO:0001217", "UniRef:UniRef50_Q4A5A4", "UniRef:UniRef90_UPI0019672E9D" ], "nt": "GTGGGAGTTGTATTTCAGTTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGAAGAGGCTAAGAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGATTATTTGCAAAGAAGTCCATTTAATTTATCGGGCGGTCAAAAACGAAGAGTTGCGTTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAGCCAACTGCCGGGCTTGATCCGCAAGGTGTCGAAGAAATGCTAAACTTATTTCACAATCTCTATTTAAATGGAAAAACGATCATTATTGTAACACATGACCTTGATAATGCTCTAAAATGA", "aa": "MGVVFQFAEYQLFESTIEKDIIFGPISMGVSKEEAKKLALKYIEMVGLPIDYLQRSPFNLSGGQKRRVALAGILAMEPKFLVLDEPTAGLDPQGVEEMLNLFHNLYLNGKTIIIVTHDLDNALK", "aa_hexdigest": "8aa26180e85591c9ad4a0a1cf01add9e", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 243342, "stop": 244217, "observations": { "mutation": [ 243516, 243537, 243570, 244014 ], "stop": [ 243516, 243537, 243570, 244014 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0019672E9D", "query_cov": 1.0080645161290323, "subject_cov": 0.40584415584415584, "identity": 0.8320000000000001, "score": 204.0, "evalue": 2.18e-63, "gene_start": 1, "gene_end": 124, "reference_start": 109, "reference_end": 233, "reference_sequence": "MQIKVEKISKEFDKKLPTHIQVLKDISVDIKEGEIISIIGHTGSGKTTFIEHLNALIIPDSGKITFSNVDVNKKQWYQSKNKLQLVKMDIVVEKTKRKIKNIKSLRKEVGVVFQFAEYQLFESTIEKDIIFGPVSMGVPVEEAKKLAKKYIKMVGLPESYLQRSPFNLSGGQKRRVALAGILAMQPKYLILDEPTAGLDPAGVEQMLQLFANLNKEEGKTVIIVTHDLDNALRWTKRCILFKNGEIIRDGETYEVLNDEQCLIENKLRATQLLTFVNKLKARGVKIGKVTDINQLAEQINNWKQNKGV" }, "paralog": false, "identity": 0.6095890410958904, "score": 327.0, "evalue": 4.1e-114, "description": "Internal stop codon at 243516, 243537, 243570, 244014. Nonsense mutation around 243516, 243537, 243570, 244014.", "psc": { "uniref90_id": "UniRef90_UPI0019672E9D", "product": "energy-coupling factor transporter ATPase", "uniref50_id": "UniRef50_Q4A5A4", "go_ids": [ "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0019672E9D", "UniRef:UniRef50_Q4A5A4", "GO:0005524", "GO:0006810", "GO:0009898", "GO:0042626" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5A4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5A4" ], "product": "Energy-coupling factor transporter ATP-binding protein EcfA2" } }, "id": "GLJLFKCOHC_450", "locus": "GCA013368035_00426" }, { "type": "cds", "sequence": "contig_1", "start": 244080, "stop": 244256, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGACGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCATTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAGGATTTAGCTGAAAAAATTAATTATTACTTAACCATGAAGCGCAAAGGAGGAACAAATGAATAA", "aa": "MSDDNFLIENNLIPTRLLSFVNELKKNNVDVGKVTSVEDLAEKINYYLTMKRKGGTNE", "aa_hexdigest": "223bb9badab2c74b88c13a5afb447dfe", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6633.472400000002, "isoelectric_point": 5.315036964416504 }, "id": "GLJLFKCOHC_451", "locus": "GCA013368035_00427" }, { "type": "cds", "sequence": "contig_1", "start": 244249, "stop": 244416, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACGTTTATTCACAAAATTGATCCTCGTTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGA", "aa": "MNKTIIGKYLNYDTFIHKIDPRFKFLTNVLLIILLFMADHFITLGILIFSIMIFY", "aa_hexdigest": "4d86250178570e8cd34577cd44f37af2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6584.976999999995, "isoelectric_point": 9.224603843688964 }, "id": "GLJLFKCOHC_452", "locus": "GCA013368035_00428" }, { "type": "cds", "sequence": "contig_1", "start": 244453, "stop": 244623, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCGCTTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGAGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCACTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGA", "aa": "MRLPLYIAIFLLIINMFTVKGAVSLNSVLSYVPLASDGNLDSYVYYRNETIINTIY", "aa_hexdigest": "934051d67e86686d6d8a16d23088c9ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6377.423500000001, "isoelectric_point": 5.875412559509278 }, "id": "GLJLFKCOHC_453", "locus": "GCA013368035_00429" }, { "type": "cds", "sequence": "contig_1", "start": 244825, "stop": 245082, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTATTTCAATATCATTACGTTTTATCCCAACATTACTTGAAGAATCTGGTCGTATCATGAAAGCGCAAGCAAGTAGAGGTATCGATTTTAAAAATGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCGAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAAAGATCTCACTATAGAGTGTTGACACCTTGTTGA", "aa": "MIISISLRFIPTLLEESGRIMKAQASRGIDFKNGNLKDKTKSFIVLIVPLFVSSFSKANDLADAMTSRGYEPYAKRSHYRVLTPC", "aa_hexdigest": "da2f200843a255fb9cb4ff44b923710f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9555.090599999994, "isoelectric_point": 9.83956775665283 }, "id": "GLJLFKCOHC_454", "locus": "GCA013368035_00430" }, { "type": "cds", "sequence": "contig_1", "start": 245420, "stop": 246892, "strand": "+", "frame": 2, "gene": null, "product": "DNA ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "nt": "TTGGCTTCCGACCTATTTAAAAAAGTAAAACATAAACGTCCGATGCTTTCATTAAATAAAGCATATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACATCTGATTTTTCTTTTTTTATCGAGCCTAAAATTGATGGTCTTTCAATTTCTATTATTTACGAAGATGGTAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTATTCAAATGTCTTGCATTCCTAAAACTATTAAATATAAGAAATCATTAGAGGTAAGAGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGGCAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCAGCAGCAGGCACATTAAGGCAATTAAATTCTCAAATTATTAAAGAACGCAATTTGTCAGCATTTTTGTATTATGTTGTGGACCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAGTTTTTAAAAACTTATGGTTTTCCTGTTACTCCTGAAGTTCAACACGCTAACACATTACAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAACTATTAATAGATTACGAAACAGATGGAGTTGTTATAAAGTTGAACGAAGTTCAATATTTTGATAAGTTAGGATCAACTGCTAAATTTCCTCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATATGCGACAGTAGGAAGAACAGGATTGGTTACGTACAATGCAGAATTAGAACCTGTAGAAATTTCTGGCAGTCTCGTAAGCTTTGCAACATTGAATAATTTTCAATATATAAAAGATTTGAATCTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAGCAATTTTGCTCAAATGATCAATGTCCTGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAGGATGGTTTAGATATTAATACTATGGGGGAGAGAAATATTGATCTATTTTTAAAAAATTCTTTAATTTCCAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAGTCTATTGAAAATATTTTGATTTCCATTGATGAATGTAAGAATAAACCTTTATATAAAATGATTTCTGCTCTTTCAATCCCACTAATTGGCGTAAAATTAGCTAAATTCGTATCCTCGAAAATTAAATCATTACATAATTTATTAAACTATGACTTTTCGCAATTAATTGATTATCATGAAATAGGAGATAAAATTATAAATTCTATAATTTCTTGA", "aa": "MASDLFKKVKHKRPMLSLNKAYSLDEIQKFIDNIKKITSDFSFFIEPKIDGLSISIIYEDGKLVRAITRGDGITGEDVTANVIQMSCIPKTIKYKKSLEVRGEVYLAISDFEKLNLKLGQENKNKLANPRNAAAGTLRQLNSQIIKERNLSAFLYYVVDPLQHNIKTMQEEHEFLKTYGFPVTPEVQHANTLQDIEEYIRNFKNQKLLIDYETDGVVIKLNEVQYFDKLGSTAKFPHYAIAFKYEPNVETTTIKRIYATVGRTGLVTYNAELEPVEISGSLVSFATLNNFQYIKDLNLNENDLVYIKKAGEIIPCVIGLISGKKEIDHFQKVEFCPYCHNKLVDSETGLEQFCSNDQCPEIQRRKLIHFCSKDGLDINTMGERNIDLFLKNSLISNPIDIFNLKNKKQDLLKLNRMGEKSIENILISIDECKNKPLYKMISALSIPLIGVKLAKFVSSKIKSLHNLLNYDFSQLIDYHEIGDKIINSIIS", "aa_hexdigest": "651f6fb3ef68878bb42da86812aee64e", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDB6CC", "query_cov": 0.9938775510204082, "subject_cov": 0.8513986013986014, "identity": 0.602, "score": 605.0, "evalue": 2.79e-211, "valid": false, "gene": "ligA", "product": "NAD-dependent DNA ligase LigA", "ec_ids": [ "6.5.1.2" ], "uniref50_id": "UniRef50_A0AAJ5IJW6", "go_ids": [ "GO:0003677", "GO:0003911", "GO:0006260", "GO:0006281" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5IJW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5IJW6" ], "product": "DNA ligase" }, "genes": [], "id": "GLJLFKCOHC_455", "locus": "GCA013368035_00431" }, { "type": "cds", "sequence": "contig_1", "start": 246983, "stop": 247204, "strand": "+", "frame": 2, "gene": null, "product": "BRCT domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "nt": "TTGGAAAATAAATCATTTGTAATAACAGGAACATTGAGACACCCTAGGTCATATTTTGAAAGGTTAATTGAAAAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAATGCCGGATCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAA", "aa": "MENKSFVITGTLRHPRSYFERLIEKNGGLIHSSISSKTNYLLAGENAGSKLQKANKLNITIITEEDLFKMIGQ", "aa_hexdigest": "a8a397ad4fae19ced4f1aa015c14e92b", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE292E9", "query_cov": 0.9863013698630136, "subject_cov": 1.0, "identity": 0.667, "score": 95.5, "evalue": 1.75e-24, "valid": false, "product": "BRCT domain-containing protein", "uniref50_id": "UniRef50_UPI002FE292E9" }, "pscc": { "uniref50_id": "UniRef50_UPI002FE292E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE292E9" ], "product": "BRCT domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_456", "locus": "GCA013368035_00432" }, { "type": "cds", "sequence": "contig_1", "start": 247181, "stop": 247828, "strand": "-", "frame": 1, "gene": null, "product": "MAG0480 family ComEC-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8", "UniRef:UniRef90_D1J7Y8" ], "nt": "TTGAATTCAAAGGAATTGGTTGTTGATTTGATTTCAATATTAATGTTATTTTTTTACCTAAGGTTATTAAATTTTTCAATATCTGCAAGTCGGGCGTTTTTGTTTTTGATATTAAAAATTGCAAGCAAAAACATTTTTAAAGGCAAATTTAATAACATTGACATATTGAGTTTTGCAGCACTATTATTGATATTCTTTGATATTAGAACCATGTATTCTTATTCTTTTATTTTATCTTTTACAATAACATTGATTATTTTTTTAATTTTAGACATTAAAAAAATTTACAAAAACAATATATTTGTAAATTTTCTATTTATTATATTACCTTATATTTCTTCTATTTCAATAGTAATTTCATTTAATCCAAAATTTAACATTTTAGGAATATTTTATCAATTATTCTTTCTACCAATTTCAATATTTAACTATGTATTTTGTTTATTTGCTTTTATAGTTAAACCTATAATAAAAATGTATGTTTCAATATTTGAAATAATAATAAATACTTTAAGCTATGGTTGTTTGATTATTGAAGTAGAAAAAAATTCAATATTAGGATATTCTATTAATTTTTATATTATTTCAATATATACATATATTAAAATAAAAAGAGAATATTATTTATTGACCAATCATTTTAAATAA", "aa": "MNSKELVVDLISILMLFFYLRLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIIINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK", "aa_hexdigest": "11573ed8ae0832150f2dfd69447994d9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 247181, "stop": 248128, "observations": { "mutation": [ 247831 ], "stop": [ 247831 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Y8", "query_cov": 1.0, "subject_cov": 0.4725274725274725, "identity": 0.991, "score": 379.0, "evalue": 9.85e-129, "gene_start": 1, "gene_end": 215, "reference_start": 241, "reference_end": 455, "reference_sequence": "MTGIGKFTRSRWKNFNYIKKSFNNLNTNLFVALSFCLYCLIIYKINIVISSIMLSLIIAVNFFNLKYWIKIAIVFPLIFLVHYLWYLDLFKINGKNICGKFEIKRFFNKSILIKYQYYNVLVKNWSSWEFNNFYKYNIELSGTLSIIKNQNMFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILKWLNSKELVVDLISILMLFFYLKLLNFSISASRAFLFLILKIASKNIFKGKFNNIDILSFAALLLIFFDIRTMYSYSFILSFTITLIIFLILDIKKIYKNNIFVNFLFIILPYISSISIVISFNPKFNILGIFYQLFFLPISIFNYVFCLFAFIVKPIIKMYVSIFEIIINTLSYGCLIIEVEKNSILGYSINFYIISIYTYIKIKREYYLLTNHFK" }, "paralog": false, "identity": 0.9936507936507937, "score": 603.0, "evalue": 2.75e-219, "description": "Internal stop codon at 247831. Nonsense mutation around 247831.", "psc": { "uniref90_id": "UniRef90_D1J7Y8", "product": "MAG0480 family ComEC-like protein", "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Y8", "UniRef:UniRef50_D1J7Y8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Y8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y8" ], "product": "ComEC/Rec2-related protein domain-containing protein" } }, "id": "GLJLFKCOHC_457", "locus": "GCA013368035_00433" }, { "type": "cds", "sequence": "contig_1", "start": 247829, "stop": 248290, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTATTTAAAATAAATGGAAAGAATATTTGTGGTAAGTTTGAAATAAAGAGATTTTTTAATAAATCAATATTAATTAAATATCAATATTATAATGTCTTAGTTAAAAATTGGAGCAGTTGGGAATTCAATAATTTTTACAAATATAATATCGAATTATCAGGCACTTTATCAATAATTAAAAATCAAAATATGTTTAGTTTAAGCAATAAAATTGATTTTGAATTATTAAATGTTAAATTGGTTTCAAAAGCATATGCATATCAAGAAAAAATTCCAATATTGCAAGATTATGATTATAAAACAAAAAAATTTCTAGCACTGGTTTTATTCAACATTCAACAAGCTAATGATTTGTTTGTAGATCAATTAAAACAACTAAGTGTTATGCACTATTTTACAATAAGTGGATTTCATTTTGGAATAATATATTTGCTTATATCAAAAATTTTAAAATGA", "aa": "MDLFKINGKNICGKFEIKRFFNKSILIKYQYYNVLVKNWSSWEFNNFYKYNIELSGTLSIIKNQNMFSLSNKIDFELLNVKLVSKAYAYQEKIPILQDYDYKTKKFLALVLFNIQQANDLFVDQLKQLSVMHYFTISGFHFGIIYLLISKILK", "aa_hexdigest": "d3274b35523b96a116cb6cd7825b0160", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18262.2427, "isoelectric_point": 9.502527046203614 }, "id": "GLJLFKCOHC_458", "locus": "GCA013368035_00434" }, { "type": "cds", "sequence": "contig_1", "start": 248545, "stop": 248985, "strand": "-", "frame": 2, "gene": null, "product": "MAG0490 family ComEA-like DNA-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9", "UniRef:UniRef90_D1J7Y9" ], "nt": "ATGAATAAACGAAAAATTTTTTTGGGACTAGGATTATTTTCATCAGCATCAATAATAATACTAATGTCAATAGGAATTAATAAATACATTTCAAATATAAAAAATAACAATAAATTATTTATCAAAAACAAAAATGAAATAATAACAATAGGAATATCGGGTGCAATAGAGTATCCCGACTCATACGAAATTAAAAAAGGCACTAAATTATTGGAAATAATAAAACAAGCACGTCTTAAAGCGGGCGCAGATTTAAACAATATTGATTTAAATTTAGAGTTAGAAAAAAATCAATCAATTAATATTCCTTTTAAAAAAGATAATTCAATTTCTATTAGAGAAGTAAATAACGTTGGCATATTAGAGGATTTAGGAATAAAAAAAGATATAGCAAAAATTATTTTAAGCTACTTCATTGCTAAGGATTATTTAATAACATGA", "aa": "MNKRKIFLGLGLFSSASIIILMSIGINKYISNIKNNNKLFIKNKNEIITIGISGAIEYPDSYEIKKGTKLLEIIKQARLKAGADLNNIDLNLELEKNQSINIPFKKDNSISIREVNNVGILEDLGIKKDIAKIILSYFIAKDYLIT", "aa_hexdigest": "923c6a228694bc699dda379080309569", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_D1J7Y9", "query_cov": 1.0, "subject_cov": 0.8588235294117647, "identity": 0.9790000000000001, "score": 266.0, "evalue": 1.55e-89, "valid": true, "product": "MAG0490 family ComEA-like DNA-binding protein", "uniref50_id": "UniRef50_D1J7Y9" }, "pscc": { "uniref50_id": "UniRef50_D1J7Y9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Y9" ], "product": "ComEA family DNA-binding protein" }, "genes": [], "id": "GLJLFKCOHC_459", "locus": "GCA013368035_00435" }, { "type": "cds", "sequence": "contig_1", "start": 249126, "stop": 249767, "strand": "-", "frame": 3, "gene": "tACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "db_xrefs": [ "COG:COG0217", "COG:KJ", "SO:0001217", "UniRef:UniRef50_Q98PU5", "UniRef:UniRef90_D1J7Z0" ], "nt": "ATGTTTCAAAAATTTTCAAAAGAAATTATTGTTGCTGCAACATTAGGCGGACCAGATCCAGACTCTAACCCATCGCTAAAATTAGCAATTGCTAAGGCTAAGGCAAAAAGTATGCCTAAAGCAAACATTGAAAAGGCAATTGCAAAAGTAGCTGGAGGATCTAAAGAAGCTGCAAACTATCAATCTTATTTATATTCAGGTACAGCATTTGGCGGTGTAAATTTTATTGTAAGTTGTTTAACAGATAATTTCAATCGTTTAAGCTCGAATATCAAACATTTCTTTTCAAAACACAATGGTCAATTAGGAAAACAAGGAATAATACCATATGTTTTTGATCAAAAAGGTTTAATTGAATTTCCAAGTAATGTTTCTGGTGAAGAAGAAACCATGATGACAGTTTTAGATGCAGGAGCAGAAGATTTTAGAAATGAAGATGGAACTTATACAATAATTACAAGTCCTTCAGCATTTCAAAAAGTAAAACAAGCATTAGACGAAACATTCAAAATTGAAAATTATACAACAGCTGAAGTTACATATATCCCAAATACCGAAATTGAAGTAGATGAAGAAAAAGCAAGCCAAATTGAACAATTTGTTGACTTTTTAGAAGACGATGAAGACGTCCAAGAAGTTTGA", "aa": "MFQKFSKEIIVAATLGGPDPDSNPSLKLAIAKAKAKSMPKANIEKAIAKVAGGSKEAANYQSYLYSGTAFGGVNFIVSCLTDNFNRLSSNIKHFFSKHNGQLGKQGIIPYVFDQKGLIEFPSNVSGEEETMMTVLDAGAEDFRNEDGTYTIITSPSAFQKVKQALDETFKIENYTTAEVTYIPNTEIEVDEEKASQIEQFVDFLEDDEDVQEV", "aa_hexdigest": "40ff67e088fb0f542b927f88be3c8775", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_D1J7Z0", "query_cov": 1.0, "subject_cov": 0.8765432098765432, "identity": 1.0, "score": 400.0, "evalue": 2.78e-140, "valid": true, "gene": "TACO1", "product": "YebC/PmpR family DNA-binding transcriptional regulator", "uniref50_id": "UniRef50_Q98PU5", "cog_id": "COG0217", "cog_category": "KJ" }, "pscc": { "uniref50_id": "UniRef50_Q98PU5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PU5" ], "product": "Probable transcriptional regulatory protein MYPU_6240" }, "genes": [ "tACO1" ], "id": "GLJLFKCOHC_460", "locus": "GCA013368035_00436" }, { "type": "cds", "sequence": "contig_1", "start": 249921, "stop": 250883, "strand": "+", "frame": 3, "gene": "holA", "product": "DNA polymerase III subunit delta", "db_xrefs": [ "COG:COG1466", "COG:L", "EC:2.7.7.7", "GO:0003677", "GO:0003887", "GO:0006260", "RefSeq:WP_175949836.1", "SO:0001217", "UniParc:UPI00158B1E7E", "UniRef:UniRef100_UPI00158B1E7E", "UniRef:UniRef50_A0A454C9E8", "UniRef:UniRef90_A0A454C9E8" ], "nt": "ATGTATTTAATAAGAGGAGAAGATAGTTATTTAATAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGACGATTATTCAGTAATTACTTACAATGAATCTATTGATTTAGAACAACTGTCAGATGATATATTTAACCAATCATTATTTTCTTTAAAAAAACAAGTCATAGTTTTAAAAAATATTGATTTTTTTAATTCTAAGAAAAAAAATGACAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGGCAAGATAACAATTTAATATTTATTTTGCAAGATATTAAAAAAAACGATATTTCTTTTAATCCTTCGTTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAATATATCTCCAACTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATTATTAATGAAGTAGATAAATTGCTATTGCAAAATAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAGCAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAAATTTATTGCACGTATTTGTTGATGGAAAATACCAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAAAACAAACAAGCATAAATTAAAAGAATTAATAATGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAA", "aa": "MYLIRGEDSYLINQELQKIIDLKFENQDDYSVITYNESIDLEQLSDDIFNQSLFSLKKQVIVLKNIDFFNSKKKNDNKKMNDFLQLLEKGQDNNLIFILQDIKKNDISFNPSLAYKKIEYISKIIEIKKPSDKDVYNYIKEYILKNGGNISPTTLLDFLSMFPNDLQQIINEVDKLLLQNKNIDMLLINNSNIISSDNIEFNLSNCILKQKSIDIIEAVDEQKKYGNGASEILSQISYLFYQIYCTYLLMENTSDNEYISKTLSIHSYRIKLFMSYIKKTNKHKLKELIMLLADIDIAIKSGLLDEENALNKFILFYIEI", "aa_hexdigest": "50136d0761945c78244cfd6d31991c97", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158B1E7E", "ncbi_nrp_id": "WP_175949836.1", "uniref100_id": "UniRef100_UPI00158B1E7E", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B1E7E", "RefSeq:WP_175949836.1", "UniRef:UniRef100_UPI00158B1E7E" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B1E7E", "uniref90_id": "UniRef90_A0A454C9E8" }, "psc": { "uniref90_id": "UniRef90_A0A454C9E8", "gene": "holA", "product": "DNA polymerase III subunit delta", "ec_ids": [ "2.7.7.7" ], "uniref50_id": "UniRef50_A0A454C9E8", "cog_id": "COG1466", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0003887", "GO:0006260" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9E8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9E8" ], "product": "DNA polymerase III subunit delta" }, "genes": [ "holA" ], "id": "GLJLFKCOHC_461", "locus": "GCA013368035_00437" }, { "type": "cds", "sequence": "contig_1", "start": 250894, "stop": 251079, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCATAGCAATTGATGATATATTATTGAAATGTTATGAAATCAATAGAAATTATGAATTTAATGATTGA", "aa": "MKKISIYELNLSTFNNKKGLNKYLSIIEKIEYLKSLNIDIIAIDDILLKCYEINRNYEFND", "aa_hexdigest": "992aac5f3fde38e0dba9e482c0ac1027", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7290.408999999998, "isoelectric_point": 6.194790840148925 }, "id": "GLJLFKCOHC_462", "locus": "GCA013368035_00438" }, { "type": "cds", "sequence": "contig_1", "start": 251086, "stop": 251490, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGATTTTAAAAAATTAGTTGAACTTTGTTTAGCAAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAACATTTAATTTCTATTTATCGAAATAAAAGCAGCGAAAATGAATCAAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTTTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTTTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGCATTAAGGGATTTATTCTTGAAGATTTTGAATTCTTAATTAATAAAAAAACAAATAACATGAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTGGTTCTATGGTTATTTGA", "aa": "MQDFKKLVELCLANNIKIMPTINIAKLKKSIIAKKHLISIYRNKSSENESNKNDIESYLLTQEFLVPTIENISNLSVFLKEIISFYSVLGIKGFILEDFEFLINKKTNNMKSKFSFLLDIYRIIKHINFGSMVI", "aa_hexdigest": "0bacb9e263fa2841619532e48fedd308", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15582.286999999988, "isoelectric_point": 9.408274269104005 }, "id": "GLJLFKCOHC_463", "locus": "GCA013368035_00439" }, { "type": "cds", "sequence": "contig_1", "start": 251782, "stop": 252267, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAAGAATATTTTTAATTTCAATAACATTAATTTTAATGAAGAAAAAAGATATTATCAATCTAGGAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCATGGACTAATAACATTCAAAATTTAGATTTTATACAGCCGAAATTGCGTAACAAAATTAATGTTGAACAACAGTTGACCAGTAAGGATAGCACATTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCAAAATATTCAATTTATTTTAAGGCTTTTCCAAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGCGAAAATAAAAAACTTTTAATTTTATTAAACTTGAATTCGTTTCCAATAAAATCATATATATGA", "aa": "MFLMLAKNSYSLYNGDELGILGTDSKNIFNFNNINFNEEKRYYQSRNISSQKYIESQIKFNPITLQIEFPWTNNIQNLDFIQPKLRNKINVEQQLTSKDSTFNFYSNLVRILNKSKYSIYFKAFPKVLLCNNLISIEYSTSENKKLLILLNLNSFPIKSYI", "aa_hexdigest": "580354c9f1d1c7d0d3f8dc4f10c9d1da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19027.607400000008, "isoelectric_point": 9.426260948181152 }, "id": "GLJLFKCOHC_464", "locus": "GCA013368035_00440" }, { "type": "cds", "sequence": "contig_1", "start": 252427, "stop": 252549, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCTGCCGTCTTAGCTTTTTTAATTTGA", "aa": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLI", "aa_hexdigest": "91c0d7081103885315333806e60440c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4222.982499999998, "isoelectric_point": 5.8228364944458 }, "id": "GLJLFKCOHC_465", "locus": "GCA013368035_00441" }, { "type": "cds", "sequence": "contig_1", "start": 252598, "stop": 252864, "strand": "+", "frame": 1, "gene": null, "product": "DUF4129 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z3", "UniRef:UniRef90_D1J7Z3" ], "nt": "ATGCAAAAGCAAAAAGAAACGTTAGCACTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACTTTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGA", "aa": "MQKQKETLALFYQYILSFYEVIKFTNQELKNFEVSISTHPMGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNL", "aa_hexdigest": "9b36e6ba1a2caa28196c4251ad6c657f", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 252427, "stop": 252996, "observations": { "mutation": [ 252547, 252862 ], "stop": [ 252547, 252862 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J7Z3", "query_cov": 1.0, "subject_cov": 0.4656084656084656, "identity": 1.0, "score": 177.0, "evalue": 3.99e-55, "gene_start": 1, "gene_end": 88, "reference_start": 58, "reference_end": 145, "reference_sequence": "MQLQFDASQVAKEQKASTVGSAIVYTLLAIVLAAVLAFLIWKLIKNKVLKKRAQKVEMQKQKETLALFYQYILSFYEVIKFTNQELKNFEVSISTHPMGEIKEGAKRLLYRLITRDDFSYSFVKNEQYKTFVKHAELINITNCNLWDKKIPETINYFKEQYELVPLGKTRDDYIQLVQKSISERFYNEK" }, "paralog": false, "identity": 0.9894179894179894, "score": 360.0, "evalue": 7.29e-130, "description": "Internal stop codon at 252547, 252862. Nonsense mutation around 252547, 252862.", "psc": { "uniref90_id": "UniRef90_D1J7Z3", "product": "DUF4129 domain-containing protein", "uniref50_id": "UniRef50_D1J7Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J7Z3", "UniRef:UniRef50_D1J7Z3" ] }, "pscc": { "uniref50_id": "UniRef50_D1J7Z3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z3" ], "product": "DUF4129 domain-containing protein" } }, "id": "GLJLFKCOHC_466", "locus": "GCA013368035_00442" }, { "type": "cds", "sequence": "contig_1", "start": 253208, "stop": 253444, "strand": "+", "frame": 2, "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "db_xrefs": [ "EC:2.7.8.5", "GO:0008444", "GO:0008654", "GO:0016020", "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F", "UniRef:UniRef90_UPI001E3AA40F" ], "nt": "ATGATAACTGATTTTTTAGATGGATTTTATGCAAGGAAAACAAAAACCGTATCCTCATTTGGAAAGATATTTGACCCAATTGCTGATAAAATTGCAACTACATTGATGTTACTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCTGTAAAGAAAAACATTGAAGTATCAGCAAATTGA", "aa": "MITDFLDGFYARKTKTVSSFGKIFDPIADKIATTLMLLFLATYNFVYIPIAILFIIRDIIVDGTRVYAVKKNIEVSAN", "aa_hexdigest": "99081ee91c5acbeb6bb892c98e69355e", "start_type": "ATG", "rbs_motif": null, "genes": [ "pgsA" ], "truncated": "both", "pseudogene": { "start": 252986, "stop": 253520, "observations": { "insertion": [ 253502 ], "mutation": [ 253046, 253178, 253442 ], "stop": [ 253046, 253178, 253442 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E3AA40F", "query_cov": 1.0, "subject_cov": 0.4406779661016949, "identity": 0.9490000000000001, "score": 143.0, "evalue": 6.13e-42, "gene_start": 1, "gene_end": 78, "reference_start": 75, "reference_end": 152, "reference_sequence": "MKNKSQKKQKIIVSKFGVANWLTVLRMLLMIPFIVFLSTSFGLILNGVQLSYKTNKGIVLSILYWINVAIFIIAMITDFLDGFYARKTKTVSSFGKIFDPIADKVATTLMLLFLATYNFVYIPIVILFIIRDIIVDGTRVYAIKKNIEVSTNWFGKIKTILVSLALVVISIGFLLYR" }, "paralog": false, "identity": 0.9548022598870056, "score": 312.0, "evalue": 3.45e-111, "description": "Internal stop codon at 253046, 253178, 253442. Frameshift due to insertion around 253502. Nonsense mutation around 253046, 253178, 253442.", "psc": { "uniref90_id": "UniRef90_UPI001E3AA40F", "gene": "pgsA", "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase", "ec_ids": [ "2.7.8.5" ], "uniref50_id": "UniRef50_UPI001E3AA40F", "go_ids": [ "GO:0008444", "GO:0008654", "GO:0016020" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E3AA40F", "EC:2.7.8.5", "UniRef:UniRef50_UPI001E3AA40F", "GO:0008444", "GO:0008654", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E3AA40F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E3AA40F" ], "product": "CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase" } }, "id": "GLJLFKCOHC_467", "locus": "GCA013368035_00443" }, { "type": "cds", "sequence": "contig_1", "start": 253903, "stop": 254247, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3", "UniRef:UniRef90_UPI000B181AC3" ], "nt": "ATGAAATCAAAGAAAATAAAGTTATTAAAGTTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAGAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCGTTTGTTGATGATGGCAATGTTCCATATGTCAATTTACTACAAATGATAAATTCTTTAGATGGATTTTTTGATAAAAACTATATAAATTTTTCGACAAATATTTTATTCTCCGAATTAAAAATGACAACTAAATTCGGATATATTTCGTTTAACTGA", "aa": "MKSKKIKLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFVDDGNVPYVNLLQMINSLDGFFDKNYINFSTNILFSELKMTTKFGYISFN", "aa_hexdigest": "e643c26441b36f46d87c462c07b50b26", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI000B181AC3", "query_cov": 1.0, "subject_cov": 0.8837209302325582, "identity": 0.982, "score": 214.0, "evalue": 2.84e-70, "valid": true, "uniref50_id": "UniRef50_UPI000B181AC3" }, "pscc": { "uniref50_id": "UniRef50_UPI000B181AC3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000B181AC3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12953.904399999983, "isoelectric_point": 8.848688697814943 }, "id": "GLJLFKCOHC_468", "locus": "GCA013368035_00444" }, { "type": "cds", "sequence": "contig_1", "start": 254266, "stop": 254793, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE", "UniRef:UniRef90_UPI0034D962DE" ], "nt": "ATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAGGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATACAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTACCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATCAAATCTAATGTGCAAGAAATTCCTCAAATTAGAAATAGCAAATTTCAAAACTCACTTATTCCCAAGGATATAGTTGAAGCAAAATTTAACTTCACTGCTTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTCTACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCAACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACCTGA", "aa": "MPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNSKFQNSLIPKDIVEAKFNFTALLIDYFYGLKEYNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHT", "aa_hexdigest": "c7d7aca0dccb228f1dfa1a775d19b126", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 253966, "stop": 254853, "observations": { "mutation": [ 254245, 254791 ], "stop": [ 254245, 254791 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D962DE", "query_cov": 1.0, "subject_cov": 0.5537974683544303, "identity": 0.983, "score": 347.0, "evalue": 1.11e-118, "gene_start": 1, "gene_end": 175, "reference_start": 122, "reference_end": 296, "reference_sequence": "MKSKKINLLKLLLTSSVAISCAILPVSCSLLSNDNDKKITIKNYEFNNLSSEYTIESKKLNLSFANDGNVPYVNLLQMINSLDGFFDKNYINFSTNILFSELKITTKFGYISFNWKTNKITMPNTYYEYITKSPGKTNFNKYLKSKSYYSKDFNKNTIVIDLKKYNFDILYHNGNVLLPLAIFNLLFCSTNYYNLYFNGNKIYGCDFDIKSNVQEIPQIRNGKFQNSLIPNDIVEAKFNFTALLIDYFYGLKENNNINSFYSTISNEQKQRMLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFY" }, "paralog": false, "identity": 0.9661016949152542, "score": 580.0, "evalue": 4.85e-213, "description": "Internal stop codon at 254245, 254791. Nonsense mutation around 254245, 254791.", "psc": { "uniref90_id": "UniRef90_UPI0034D962DE", "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D962DE", "UniRef:UniRef50_UPI0034D962DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D962DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D962DE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 20802.418900000008, "isoelectric_point": 9.074070167541507 }, "id": "GLJLFKCOHC_469", "locus": "GCA013368035_00445" }, { "type": "cds", "sequence": "contig_1", "start": 254800, "stop": 254928, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4", "UniRef:UniRef90_UPI001E356EC4" ], "nt": "ATGCCTTCATTTTATAATCACAGAGATTCTAGCATTGCAAAAGATTTCTATGAATATACTACAAATAAAAGAGAAAGAATTTATCAATCTACACGAAAATATCTAAAAAATAATAAATTAAATAATTGA", "aa": "MPSFYNHRDSSIAKDFYEYTTNKRERIYQSTRKYLKNNKLNN", "aa_hexdigest": "3ed931e83391c74a544932b8aa5bfc3b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 254716, "stop": 254910, "observations": { "mutation": [ 254791 ], "stop": [ 254791 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E356EC4", "query_cov": 0.8571428571428571, "subject_cov": 0.5373134328358209, "identity": 0.9440000000000001, "score": 72.0, "evalue": 8.37e-16, "gene_start": 1, "gene_end": 36, "reference_start": 29, "reference_end": 64, "reference_sequence": "MLSQNPVISTKEYIKYLYNLNDLHTWVSMPSFYNRKDSSIAKDFYEYTTNKRERIYQSTRKYLKIIN" }, "paralog": false, "identity": 0.953125, "score": 126.0, "evalue": 5.03e-40, "description": "Internal stop codon at 254791. Nonsense mutation around 254791.", "psc": { "uniref90_id": "UniRef90_UPI001E356EC4", "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E356EC4", "UniRef:UniRef50_UPI001E356EC4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E356EC4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E356EC4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5235.760500000001, "isoelectric_point": 9.819453620910643 }, "id": "GLJLFKCOHC_470", "locus": "GCA013368035_00446" }, { "type": "cds", "sequence": "contig_1", "start": 255103, "stop": 255342, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAATATTATTGTAGATTTATCAACTAATAGTGGCGGCAATATTGGCGCTATGCAAAGGGGATTGGGTTTTATTACAGATGAACCAATATTAAACAAAACATATAATCGTTTAGATGAAGAAATAAATATTAGATCTGTTGAAGTTGATACAGATGGCGATGGAGATTACAAAAACGATGCCTATTCTGAATACAATTGA", "aa": "MKLIKKNLRNMKIKNIIVDLSTNSGGNIGAMQRGLGFITDEPILNKTYNRLDEEINIRSVEVDTDGDGDYKNDAYSEYN", "aa_hexdigest": "1efe6fbcb76a50a6008156400ba2ef52", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8954.955099999994, "isoelectric_point": 4.98173313140869 }, "id": "GLJLFKCOHC_471", "locus": "GCA013368035_00447" }, { "type": "cds", "sequence": "contig_1", "start": 255406, "stop": 255636, "strand": "+", "frame": 1, "gene": null, "product": "S41 family peptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3", "UniRef:UniRef90_UPI0005133DA3" ], "nt": "ATGAAATTGGGCAAGATAATAGGACAAAAAACAGGTGGTGGTGCAGCATCAATTTTACCTGTTGTTTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATGATTTATCCGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGTGGAATAGAACCTGATATTTACTTAGAATACAAAGATTTTTATAATGATCAAAAACTATTAGAAATAATAAAAAATAAATAA", "aa": "MKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNDLSVTMKNNTIKSIEGGIEPDIYLEYKDFYNDQKLLEIIKNK", "aa_hexdigest": "db607b7cb8c0fc7b1eb0fb0be93a9613", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 255304, "stop": 255636, "observations": { "mutation": [ 255340 ], "stop": [ 255340 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005133DA3", "query_cov": 1.0, "subject_cov": 0.628099173553719, "identity": 0.9740000000000001, "score": 146.0, "evalue": 6.24e-44, "gene_start": 1, "gene_end": 76, "reference_start": 46, "reference_end": 121, "reference_sequence": "MGLTPHFSTLPGDYKNDAYSEYNWYLLTGVNTFSAANTFTSIFKEMKLGKIIGQKTGGGAASILPVVLPDGTSIVISSNNLSVTMKNNTIKSIEGGIKPDIYLEYKDFYNDQKLLEIIKNK" }, "paralog": false, "identity": 0.9727272727272728, "score": 213.0, "evalue": 7.51e-73, "description": "Internal stop codon at 255340. Nonsense mutation around 255340.", "psc": { "uniref90_id": "UniRef90_UPI0005133DA3", "product": "S41 family peptidase", "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005133DA3", "UniRef:UniRef50_UPI0005133DA3" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0005133DA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0005133DA3" ], "product": "S41 family peptidase" } }, "id": "GLJLFKCOHC_472", "locus": "GCA013368035_00448" }, { "type": "cds", "sequence": "contig_1", "start": 256115, "stop": 256300, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATTCAAATAAAATAACAAAACAAGAATATGAAATTATTTTTAATGAATTTAAAGGAATAATATTGATATTTAAGCAACTAATCACAAATATTTATAGTAAAGATTTAAAAAACGTTTTAACATCTCAACTACCAAAAGTTTTTAAAGCAATAAATAATGCTAAAAAAAGTGCTAAATAA", "aa": "MVNSNKITKQEYEIIFNEFKGIILIFKQLITNIYSKDLKNVLTSQLPKVFKAINNAKKSAK", "aa_hexdigest": "f5520905232713a6deb00746ece17446", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7060.3039000000035, "isoelectric_point": 9.933240318298338 }, "id": "GLJLFKCOHC_473", "locus": "GCA013368035_00449" }, { "type": "cds", "sequence": "contig_1", "start": 256385, "stop": 256654, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E316352", "UniRef:UniRef90_UPI001E316352" ], "nt": "ATGTTCAAAGAATTTAAAAATGGAACTGATGTTTTTATTGAATATTTAACTCCAATATTGAATAGCTTTAAAGAAAAACATGAATTTTTAAAAACAAATGATAGCAAATATAATGAATTAAAAAACATGTTAATAAAACATTTAGAATATATTTTGGATTACTTTTGCAACCTTAAATTTGATTATTTATTCCACAATGATGGTCCAAAACAAACAGAATATGATAATAAAGGTGGAGGAAAGAAAGTATTGATATTATTTGGTGGATGA", "aa": "MFKEFKNGTDVFIEYLTPILNSFKEKHEFLKTNDSKYNELKNMLIKHLEYILDYFCNLKFDYLFHNDGPKQTEYDNKGGGKKVLILFGG", "aa_hexdigest": "92ccae50c1222a3965913b3e4c474430", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 256318, "stop": 256780, "observations": { "insertion": [ 256339 ], "mutation": [ 256387, 256666, 256693 ], "stop": [ 256387, 256666, 256693 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E316352", "query_cov": 1.0, "subject_cov": 0.6013513513513513, "identity": 0.899, "score": 175.0, "evalue": 9.36e-55, "gene_start": 1, "gene_end": 89, "reference_start": 38, "reference_end": 126, "reference_sequence": "MLNVLLELGIDDKVSKGLRNLTRDWVLSKTVGAWAIKMFKEFKNGTDVFIEYLTPILNSFKEKHEFLKTNDSKYNELKSMLIEHLKYILDYFCNLKFDYLFHNEGPKQSQYDKKGGGKMVLLLFGGWPILFDVEKFIEPKYSIHPAMI" }, "paralog": false, "identity": 0.8627450980392157, "score": 259.0, "evalue": 2.55e-90, "description": "Internal stop codon at 256387, 256666, 256693. Frameshift due to insertion around 256339. Nonsense mutation around 256387, 256666, 256693.", "psc": { "uniref90_id": "UniRef90_UPI001E316352", "uniref50_id": "UniRef50_UPI001E316352", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E316352", "UniRef:UniRef50_UPI001E316352" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E316352", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E316352" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10581.02709999999, "isoelectric_point": 6.821611213684082 }, "id": "GLJLFKCOHC_474", "locus": "GCA013368035_00450" }, { "type": "cds", "sequence": "contig_1", "start": 256691, "stop": 257758, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAACGAAATTTAAAAAATTTTGCAAATAAGTTTGATTTTGATTTTAAAAATGACAACAAAAAAGAATTAGCAAAATTAATGACAATAGATGAATCGATAAATAAAGGCTACAATTTCTTTTTTAAAGACAAAATTGATTCTGGTAGTGGAAAAGAATCTTATATTATTGATGATTATAAAATTCAAAAAGATTATAAACTATTGGCAAGTGAAAAAGAAGATACTATCAATATACAAATAACGTTTAAGCTAAATGATTATAGTGAAATAAAAACCTTTACTAAATCAGGTTTCAATGACAAAGCAAGCATTGAAAAAATAAAACAAGAATTAGAAAATGAACCAAAAAGGCTTGACGATTTATTGGATAAACTTACAATGGAATTCACTAAAAATAATGATTTTGAAAAGCAATCTATTGATGTTAAAGATATTACAATTCAAGATTATAGATTTAATCTAAAAGATAGTGATTTTGCAGCTATCTATGAAATAGAGGCGGTTAAATATGATAAAACATTGGGAAAATTAACTTATAAAATTAAGTTAAAGTCAAATAAAAGTTTATTGAATGAATATAGTAAAAATCCAATAGAATCTACTAAATCAAGAACATTTGAATTTTATGGATTTGCTAAGGATATAAGCGAAATAACTAAAAATTTAGAATCGGATGTAAAAAAATCAAATGAATTTAAAAAATATCTAGAAAATGTTAAATTAAATATTGAAACAAATATTGAAAAAAAGATTAATGAAGCAAGAAAAGAACAAGGAAAACAAGCCGCTCATGCAAACGTAATTAAAGCAATAATTGAACAATATAAAAAGTTTAACGAAGAAATAGAAAAAACGTTATTTGAACTAGCAAATAAAGCAATTAATGTATTTGGTCAATTTAATTCTAACAAAGAATCTATTAAAAAACTTTACAACATCTTAATTAATTTAATTATTAACACCAATGATGAAATGTTAAATGTATTATTAGAATTAGGCATTGATGATAAAGTTTCTAATGGTAGGGGTTTTATGTATTATAAAAATCTGACAGCAGACTGA", "aa": "MNKRNLKNFANKFDFDFKNDNKKELAKLMTIDESINKGYNFFFKDKIDSGSGKESYIIDDYKIQKDYKLLASEKEDTINIQITFKLNDYSEIKTFTKSGFNDKASIEKIKQELENEPKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITIQDYRFNLKDSDFAAIYEIEAVKYDKTLGKLTYKIKLKSNKSLLNEYSKNPIESTKSRTFEFYGFAKDISEITKNLESDVKKSNEFKKYLENVKLNIETNIEKKINEARKEQGKQAAHANVIKAIIEQYKKFNEEIEKTLFELANKAINVFGQFNSNKESIKKLYNILINLIINTNDEMLNVLLELGIDDKVSNGRGFMYYKNLTAD", "aa_hexdigest": "d8c125e6e7e83271da6735f4126d747e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 41562.729399999975, "isoelectric_point": 8.175187492370608 }, "id": "GLJLFKCOHC_475", "locus": "GCA013368035_00451" }, { "type": "cds", "sequence": "contig_1", "start": 257858, "stop": 258346, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATACATTAGTTGAACAAGAAAACATTATATCTTTAAAAAATAATGAATATTTAACATCTAAAAAATCTTCAATGATTTCAGAAAATGACTTTGAATTAAACAAGCAAAAAAGTAATGAACTAGCAGGGTTGGAATTAAATATTTCAGAAATCAAGGCCAATGCCAATGGAAAATTAAAAATAACCTTTTCGTTATTGAATAAAACATTTAAACATGGAACAAAAGAATCAAACAAAAAGACAGTTACATTCAATTTCTTAAGTGATGAAAATTACACATTTAATTTAATTGATAACTTAAGCATTTCAAAAGAATCAACTTATGTAACTGATGGTGTTTATACTGAAGCGGATTTTAACAATAAAATTGCCAATAATAAACAAGAGATTGCTAAGTTGTTAGGTATTTCTGATTTTCTAAATCAAAATTCAATTATCGAATTGAATCAAGAAAGCACAAACGCTATTGTGTCATTAAAAAAATGA", "aa": "MDTLVEQENIISLKNNEYLTSKKSSMISENDFELNKQKSNELAGLELNISEIKANANGKLKITFSLLNKTFKHGTKESNKKTVTFNFLSDENYTFNLIDNLSISKESTYVTDGVYTEADFNNKIANNKQEIAKLLGISDFLNQNSIIELNQESTNAIVSLKK", "aa_hexdigest": "7841f256e44c90ca9e42a6e639c16583", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18283.291999999994, "isoelectric_point": 5.3109445571899405 }, "id": "GLJLFKCOHC_476", "locus": "GCA013368035_00452" }, { "type": "cds", "sequence": "contig_1", "start": 258467, "stop": 259624, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34", "UniRef:UniRef90_UPI0005CB0F1B" ], "nt": "GTGGGATTTAATACATTACTCAACATAGAAAGACAAGAAACATCAAAGAAACTTTCTAATTTAGTTAATTCAATTGATCTGAACGAATTTTTGGCACCTGAAAAGCATAACAAAAAACCTTCAGAAATCAAAAATATTGATTTCAATAAAAAAAATATTGATGGAGCAATGTTAGTTATTGAAAAAATGCAAGCCAATGACGAAGAAGGAACATTGTTAATTAGTTTTTATTTAAAAAGTTCTAACCCAAAATTCAGCGATTTAAAAACAGAAATTAAAACAGTAAAATTGACAGATTTCTTAACAAATGCACAATTTATTAATAACCAAAGAAATGAAACAATTGCTTATTTAAATGAATTAATAGGAAATTTAAATATAAAAGTAAGCGATAAGAAACCTGAAACTACTATTAAAAGATTTGCATCTTCAATCAAGCAATCAGATTTAAATATTGAAATTCCAAAGCCAAATAATAATTTTAAAAATATTGAAGTTGTTGTAAAGCAATTAATTCCAAACGATGAAAGTGGAAAATTAAGTATAAGATTAGCAATCAAACAATTGAACAAACAAATTAATGAATATTGCGAATCAAGCAAACTTATTGAAGTTGAATTAAGTGGTTATAAAACTAAAACAATGGATAAAAATGAAAAAATAGAGTCTTTAAATAAAGTTGCTAAAGAAACAACAATGTTAAGATTAATTTCTTCAAAAAATATTGCCAAAACATTTGCTTCCGATGTTCAAATAAACGATTTTGAAACAGAAATTAGCAATGATTTTTATATTGAAATTTTAGAAATAAGTGAATCAAATGATGATAAAGGAAACTTAAAAATTAAATTTGTTGTTAAAACAAACGAAAAACAATATAAAGATTTAAAATCAAATGAGCAAACTATAACATTGTCAGGTTTTTATACAAAAGAACAATACTTAAAAGCTTGTAAGCAAGAGTTGAATAAATTAATTAGCGATGATAAGATAGAAGCAAATCTAGTTTTTGATAAAGAAAATGATAAGTCAAATATATTACCTTCGAATGTTGAAGATAAAGAAATTCATCTAAATATAAGCGATTCAAAATATACTATTGAAGGAAATCCATCAATTATTGCAGATGATGATCAAGGAATTTTGTCAGTAAGTTGA", "aa": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLAPEKHNKKPSEIKNIDFNKKNIDGAMLVIEKMQANDEEGTLLISFYLKSSNPKFSDLKTEIKTVKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETTIKRFASSIKQSDLNIEIPKPNNNFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKLIEVELSGYKTKTMDKNEKIESLNKVAKETTMLRLISSKNIAKTFASDVQINDFETEISNDFYIEILEISESNDDKGNLKIKFVVKTNEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEDKEIHLNISDSKYTIEGNPSIIADDDQGILSVS", "aa_hexdigest": "99168b792968e3eb107ef9026cb1b37a", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 258164, "stop": 259624, "observations": { "mutation": [ 258469 ], "stop": [ 258469 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "query_cov": 1.0, "subject_cov": 0.43209876543209874, "identity": 0.971, "score": 699.0, "evalue": 1.06e-245, "gene_start": 1, "gene_end": 385, "reference_start": 1, "reference_end": 385, "reference_sequence": "MGFNTLLNIERQETSKKLSNLVNSIDLNEFLALEKHNKKPSEIKNVDFNKKNIDGAMLVIEKMQANDEEGTLLISFYLKSTNPKFSDLKTEIKTIKLTDFLTNAQFINNQRNETIAYLNELIGNLNIKVSDKKPETAIKRFASSIKQSDLNIEIPKPNNHFKNIEVVVKQLIPNDESGKLSIRLAIKQLNKQINEYCESSKFIEVELSGYKTKTMDKNEKIESLNKVAKETTTLRLISSKNIAKTFASDVQINDFETKISNDFYIEILEISESNDDKGNLKIKFVVKTKEKQYKDLKSNEQTITLSGFYTKEQYLKACKQELNKLISDDKIEANLVFDKENDKSNILPSNVEGKEIHLNISDSKYTIEGNPSIIADDDQGILSVSWTIKVKEEKFKTFIVSESRISKKITNFLTKKQNEINIEQKRLDTLVEQENITSLKNNEYLTSKKSSMISKNDFELNKQKSNELAGLELNISEINANANGKLEIAFSLLNKTFKHGTIESNKKTVTFNFLSDENYTFNLINNLSISKESTYVTDGVYTKADFKNKIANNKQEIAKLLGISDFLNQNSIIELNQENENAIVSLNKFVNTTTVKYEFILKIKSANDKLFKKFSGEISIANIMNKRNLKNFANNFDFDFKDYTKKELAKSMTIDESIEKGYNFFFREKINFGSGEESYIIDDFKIQKDYKLLASENANDINIQITFKLDDYSEIKTFTKSGFNDKASIEKIKEELKNESKRLDDLLDKLTMEFTKNNDFEKQSIDVKDITINDYKFNLKDSDFADIYEIEAVEYDKTLGKLTYKIKLKTKKSLLKEYSKNPIESTKSRTFEFNGFAKDISEITKNLESDVKKSDEFKKYLENVKLNIETNIEKKINEAKKNNKKKPLIKT" }, "paralog": false, "identity": 0.9526748971193416, "score": 853.0, "evalue": 1.25e-308, "description": "Internal stop codon at 258469. Nonsense mutation around 258469.", "psc": { "uniref90_id": "UniRef90_UPI0005CB0F1B", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0005CB0F1B", "UniRef:UniRef50_UPI0034A1CB34" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1CB34", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1CB34" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "GLJLFKCOHC_477", "locus": "GCA013368035_00453" }, { "type": "cds", "sequence": "contig_1", "start": 260063, "stop": 260842, "strand": "-", "frame": 1, "gene": null, "product": "lipoprotein 17-related variable surface protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC", "UniRef:UniRef90_UPI00193A2EAC" ], "nt": "ATGAAAAAGAATACAAAAATCAAAAGCATTTCCATTTTAAGTGCTACTGCCATTTTACTTGTATCAGGAGTCATTTCATCAGCCTGCAACAAGACGCAAGATAAAGAAACTGAAACAACAAAATTAAAAGAACAAACAAGAAATATTAAACCAAATTTAAAACTTTCTATTAAACAAGATAACATACTTCCAAGTGAAATTAATTCATCAAATTTTTATGTCGATAATCTTGATAAAGAAATATCGTTTGAAATCTCTAATATTATTGCAGATGATGTAAACGGGAATGTTATTATAAATTATTATTTTACAAAATCAATAATCGATTCTAAAACAAGACAACCACTGTTAATAAAATCAAATATGTTTTCTCAAAAATACACAGGATTCAAAGCGTTGGTTGCCCCTTTAAATTCGTCAATTGAACAAGTTAATCTAGAAATAAATGAATTTAATAAAAAAGACTTTTTGCCTTCGCAATATATTAAATTGCAAAGCAATTTTTCATTTAAAAACATCGATTCCCAATATGAATTAAAAACTGAATATATTGCAAACGATGAACAAGGCAAAATAGAAGTTAAATATTATTATGTACAAAAAGACAAAGATATAAAATCAAAAATTATTTCAAAAACGTTTTATGGTTTTAATTCATTGAATAATATCGTTAATATGCAAAAAGAAGCTAACGATTTAAATAAGTTAGGAAATGAGATATCTTTAAAATTAAAGAAACAAATTAATAAATCAGAAACATATGCTTCAACATTGAATTGA", "aa": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDNILPSEINSSNFYVDNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNMFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDEQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIVNMQKEANDLNKLGNEISLKLKKQINKSETYASTLN", "aa_hexdigest": "a71aef9648b3cda5b53f4c972af3cf0a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 259760, "stop": 260842, "observations": { "mutation": [ 259918, 260065 ], "stop": [ 259918, 260065 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A2EAC", "query_cov": 1.0, "subject_cov": 0.6675257731958762, "identity": 0.973, "score": 462.0, "evalue": 9.29e-162, "gene_start": 1, "gene_end": 259, "reference_start": 1, "reference_end": 259, "reference_sequence": "MKKNTKIKSISILSATAILLVSGVISSACNKTQDKETETTKLKEQTRNIKPNLKLSIKQDKILPSEINLSNFYVNNLDKEISFEISNIIADDVNGNVIINYYFTKSIIDSKTRQPLLIKSNTFSQKYTGFKALVAPLNSSIEQVNLEINEFNKKDFLPSQYIKLQSNFSFKNIDSQYELKTEYIANDGQGKIEVKYYYVQKDKDIKSKIISKTFYGFNSLNNIANMQKEANTLNKLGNEISLKLKKQINKSETYASTLNWSDFETSKKLNSAYLRSNGELIFDDINGEVKLNYYFEKQYIDKKTGASYWIKSKVYTTKITGFKSFLKNLNIAVDKVNLSINEKNINTILPSQYIQNLSNFKFDNLNNEYELQKKFSYDDQKGELTISY" }, "paralog": false, "identity": 0.9722222222222222, "score": 675.0, "evalue": 3.22e-248, "description": "Internal stop codon at 259918, 260065. Nonsense mutation around 259918, 260065.", "psc": { "uniref90_id": "UniRef90_UPI00193A2EAC", "product": "lipoprotein 17-related variable surface protein", "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A2EAC", "UniRef:UniRef50_UPI00193A2EAC" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A2EAC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A2EAC" ], "product": "lipoprotein 17-related variable surface protein" } }, "id": "GLJLFKCOHC_478", "locus": "GCA013368035_00454" }, { "type": "cds", "sequence": "contig_1", "start": 260925, "stop": 261929, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175949841.1", "SO:0001217", "UniParc:UPI00158BBEFD", "UniRef:UniRef100_UPI00158BBEFD", "UniRef:UniRef50_D1J7Z7", "UniRef:UniRef90_D1J7Z7" ], "nt": "ATGAAAAAATCCAAAAAAATTTTATTAACTATGGGGGTAGCACTTCCATTATTAGCCTCAACTCCATTGGTTGCTGCAGGTTGCGTAAAAACTAAACCAGAAGTTAAACCAGAAGATCAAAAGAATCCTGAAGGTGAAAAACCAGGAACAACACCCGAAAAGAATCCCGAAGGTGAAAAACCAGGAACAACACCTGAAGTTAAACCAGAAGAAAAAAACGAACTTCAAAAAGCAACTGATGGTATTGCAGATGCTACAGTTAAAGCAAATTTTGAAAGTGAATTAAAGAAATATCGCGAAGAATTAAAAACTAAATCAGAAGCCATTTTAAAGCCAATTATTGAAAATAAAGAAATTAAAGCTGCAGTTTCTGAAAAAGAATTGCCAAATATTACAAAAACATATAATGAAGCAATTTCTAAATTAAATGAAATTTTAGATGATATTATTTTGTCAACATTAAAAGAATACAACACAAATAAAACAAAAGTTTCTGATGGATTAATGACAATTAAAAATTATTTAACAAAAGAAAAAATTTCTGATTTAATTAAAAAAGAAAACGGATTGGCAGATTTCTTGGTAGCATTTGGAAAAAATATTTCTAAATATGCAAAAGTAATTTTTGAAACACTAAAAACAAACGAATCAACAAAAGCAATAGCTCAATTAAACGATTTAATTACAGCTGTTGACAATAAATTTGCTGGAAAAGATAATTTATTTGAAACAACCGCTAAGAAATTAAAAGAATTTGATTTTAGCAAATTGGCTGAAGAAGGATATGTAAAAACATTTGAAAAATTAATTACATTATTATCTGACGCTAAGGCAGAAGCAGTTGCGGTATTAAAATCTGTAAAAATGTCTGAAGAAGATATTAAAAAAGTAAAAGACGTAATTAGACCAATTTACAACGTTTTAAATGAAGGTCTAAAAAACTCAATTAACTTATTTGAACAATCATTGAGAGATTTACCAAAGGTGTTAGAAATCTTAAAATAA", "aa": "MKKSKKILLTMGVALPLLASTPLVAAGCVKTKPEVKPEDQKNPEGEKPGTTPEKNPEGEKPGTTPEVKPEEKNELQKATDGIADATVKANFESELKKYREELKTKSEAILKPIIENKEIKAAVSEKELPNITKTYNEAISKLNEILDDIILSTLKEYNTNKTKVSDGLMTIKNYLTKEKISDLIKKENGLADFLVAFGKNISKYAKVIFETLKTNESTKAIAQLNDLITAVDNKFAGKDNLFETTAKKLKEFDFSKLAEEGYVKTFEKLITLLSDAKAEAVAVLKSVKMSEEDIKKVKDVIRPIYNVLNEGLKNSINLFEQSLRDLPKVLEILK", "aa_hexdigest": "a79eb386e423d1884869481979527ff0", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158BBEFD", "ncbi_nrp_id": "WP_175949841.1", "uniref100_id": "UniRef100_UPI00158BBEFD", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158BBEFD", "RefSeq:WP_175949841.1", "UniRef:UniRef100_UPI00158BBEFD" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158BBEFD", "uniref90_id": "UniRef90_D1J7Z7" }, "psc": { "uniref90_id": "UniRef90_D1J7Z7", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J7Z7" }, "pscc": { "uniref50_id": "UniRef50_D1J7Z7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J7Z7" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_479", "locus": "GCA013368035_00455" }, { "type": "cds", "sequence": "contig_1", "start": 262289, "stop": 262441, "strand": "+", "frame": 2, "gene": "rpmG", "product": "50S ribosomal protein L33", "db_xrefs": [ "COG:COG0267", "COG:J", "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904", "RefSeq:WP_012855488.1", "SO:0001217", "UniParc:UPI0001BC21A4", "UniRef:UniRef100_D1J7Z8", "UniRef:UniRef50_P35871", "UniRef:UniRef90_B3PMG6" ], "nt": "ATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAA", "aa": "MPREGLTLRCEKCKMENYITKKNKKLHPDKMEVQKYCSKCNAHTTHKEKK", "aa_hexdigest": "a94740e22d2064ea07f0b499858aa7c7", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC21A4", "ncbi_nrp_id": "WP_012855488.1", "uniref100_id": "UniRef100_D1J7Z8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21A4", "RefSeq:WP_012855488.1", "UniRef:UniRef100_D1J7Z8" ] }, "ips": { "uniref100_id": "UniRef100_D1J7Z8", "uniref90_id": "UniRef90_B3PMG6" }, "psc": { "uniref90_id": "UniRef90_B3PMG6", "gene": "rpmG", "product": "50S ribosomal protein L33", "uniref50_id": "UniRef50_P35871", "cog_id": "COG0267", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005737", "GO:0005840", "GO:0006412", "GO:1990904" ] }, "pscc": { "uniref50_id": "UniRef50_P35871", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P35871" ], "product": "Large ribosomal subunit protein bL33" }, "genes": [ "rpmG" ], "id": "GLJLFKCOHC_480", "locus": "GCA013368035_00456" }, { "type": "cds", "sequence": "contig_1", "start": 262722, "stop": 263132, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "TTGGCTAAAAAGAAATATGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTCGAAACATTTGAAAATTTAAAATCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGCAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGTACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGA", "aa": "MAKKKYATVGVEKDYLILETFENLKSMLPKSKFVLVNGQEFRILKDEEEYSNVQEACNISLQSFEQIKTVLTEGITELEVSNKLGYLMRLFGAEKESFETIVAFGTNAAEPHHHPTDRKLADGDIVKIDFGAQYQG", "aa_hexdigest": "01e59e4a40b1bf253a9a3c195050a784", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 87, "aa_cov": 0.625, "hmm_cov": 1.0, "evalue": 2.0989882900916996e-22, "score": 75.3111572265625, "start": 52, "stop": 136 } ], "seq_stats": { "molecular_weight": 15395.32599999998, "isoelectric_point": 5.111382865905762 }, "id": "GLJLFKCOHC_481", "locus": "GCA013368035_00457" }, { "type": "cds", "sequence": "contig_1", "start": 263409, "stop": 263534, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00557.29" ], "nt": "ATGATTATTACTGTTGAACCTGGTATATATATCGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAAAGTATTAAGCAGACCCGAAGATTATGAAAAATAA", "aa": "MIITVEPGIYIENEGGARIEDTILVTKDGSKVLSRPEDYEK", "aa_hexdigest": "4b564575859134acf0642200976ffa80", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00557.29", "name": "Peptidase_M24", "length": 28, "aa_cov": 0.6585365853658537, "hmm_cov": 1.0, "evalue": 1.057617325529744e-09, "score": 33.81732940673828, "start": 1, "stop": 27 } ], "seq_stats": { "molecular_weight": 4580.1284000000005, "isoelectric_point": 4.442785835266113 }, "id": "GLJLFKCOHC_482", "locus": "GCA013368035_00458" }, { "type": "cds", "sequence": "contig_1", "start": 264129, "stop": 264407, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTATTCATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGCGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGTTATAAAAGCACCATGAATAACCTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCGAGAAAAATAACTTCAATAACTGATTTAATATTAAAATAG", "aa": "MLFISEYPSNYHTSKITLKARNRISAAISNYLVLLSCYKSTMNNLIDQFLNIGKEVYCLDDIQNNLNEDNFNKNLINEGARKITSITDLILK", "aa_hexdigest": "a5db2ba9b25349b8803ac4c2f9fbe82a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10577.022299999986, "isoelectric_point": 7.7458700180053714 }, "id": "GLJLFKCOHC_483", "locus": "GCA013368035_00459" }, { "type": "cds", "sequence": "contig_1", "start": 264399, "stop": 264644, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01261.30" ], "nt": "TTGCCTTTAATTAGAGTTATTCATTTAAATGATTCTATGAATGAACTTGGATCAAAAAAAGATCGTCATGCAAACATTGATAAAGGCCATATAGGTTTAGAAACATTGAAAAAATTTGTTCATGATAAAGACTTCGATAATATTCCTATTATTTTAGAAACACCGTTTATTAATAATAAAAGTCCTTACAAAGATGAAATAATAATGCTTTTAGAGGATAATAAAAAAGATAATATTCTATTTTAA", "aa": "MPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLETLKKFVHDKDFDNIPIILETPFINNKSPYKDEIIMLLEDNKKDNILF", "aa_hexdigest": "567047d368d06fca06c9b69f8ff18e2a", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387AC6AF", "query_cov": 0.8641975308641975, "subject_cov": 1.0144927536231885, "identity": 0.7709999999999999, "score": 105.0, "evalue": 3.43e-28, "valid": false, "product": "TIM barrel protein", "uniref50_id": "UniRef50_Q49058" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01261.30", "name": "AP_endonuc_2", "length": 67, "aa_cov": 0.8148148148148148, "hmm_cov": 0.9850746268656716, "evalue": 7.786052662108182e-15, "score": 50.6086540222168, "start": 4, "stop": 69 } ], "seq_stats": { "molecular_weight": 9455.846999999992, "isoelectric_point": 6.0339365005493155 }, "id": "GLJLFKCOHC_484", "locus": "GCA013368035_00460" }, { "type": "cds", "sequence": "contig_1", "start": 264708, "stop": 265250, "strand": "-", "frame": 3, "gene": "nfo", "product": "deoxyribonuclease IV", "db_xrefs": [ "COG:COG0648", "COG:L", "EC:3.1.21.2", "GO:0003677", "GO:0006281", "GO:0008270", "SO:0001217", "UniRef:UniRef50_Q4A6S5", "UniRef:UniRef90_D1J801" ], "nt": "ATGATTAAATTAGGAAGCCATATATCATTTAAAAAACCCGATTATTTAATAGGTTCATTTAATGAAGCTATTAATAATGGCGAGAATTGTTTTATGATTTACTTAGGAGCACCACAAAGTGCTTTTAGAGTTGATAGTTCATTGTATAATATTGAAAAATATTTAAAGCAATGCAATAGTATTATTCCTGTATCTGATATTGTGGTTCATGCCCCATATATAGTAAATTTAGCCAATAGAGAAAAGGCAGATTTTGGGATTAAATTTTTATGTAGTGAAATTAAAAGAATGAATAGTTTTGGTGCTAAATTGTTAGTTTTACACCCTGGTTCATCCGTTGAATATAGCAGAAGCGAAGCATTAGCATCATTAATTCAATCTCTTAAAAAGATAATTGAATCTACTAAGGACGTAATAATATGTCTTGAAATAATGTCAGGTAAAGGCAGTCAAATTTGCAGCAATTTCAATGACTTATTATACGTGATAAAATCCGTTAATTCCGAAAGAATTGCAATATGTTTTGATACTTGCCATGCCTGA", "aa": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKADFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICFDTCHA", "aa_hexdigest": "5580119ba68d62cdb43ed64d89fd637d", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "nfo" ], "truncated": "5-prime", "pseudogene": { "start": 264405, "stop": 265250, "observations": { "mutation": [ 264710 ], "stop": [ 264710 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J801", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.9890000000000001, "score": 354.0, "evalue": 3.98e-122, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MIKLGSHISFKKPDYLIGSFNEAINNGENCFMIYLGAPQSAFRVDSSLYNIEKYLKQCNSIIPVSDIVVHAPYIVNLANREKVDFGIKFLCSEIKRMNSFGAKLLVLHPGSSVEYSRSEALASLIQSLKKIIESTKDVIICLEIMSGKGSQICSNFNDLLYVIKSVNSERIAICLDTCHAWDAGYDIKDYENFKSYIKQQNILPLIRVIHLNDSMNELGSKKDRHANIDKGHIGLETLKKFVHDKDFDNIPIILETPFINNKNPYKDEIIMLLEDNKKDNILF" }, "paralog": false, "identity": 0.9857651245551602, "score": 555.0, "evalue": 9.73e-204, "description": "Internal stop codon at 264710. Nonsense mutation around 264710.", "psc": { "uniref90_id": "UniRef90_D1J801", "gene": "Nfo", "product": "deoxyribonuclease IV", "ec_ids": [ "3.1.21.2" ], "uniref50_id": "UniRef50_Q4A6S5", "cog_id": "COG0648", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0008270" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J801", "EC:3.1.21.2", "UniRef:UniRef50_Q4A6S5", "COG:COG0648", "COG:L", "GO:0003677", "GO:0006281", "GO:0008270" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6S5" ], "product": "Probable endonuclease 4" } }, "id": "GLJLFKCOHC_485", "locus": "GCA013368035_00461" }, { "type": "cds", "sequence": "contig_1", "start": 265417, "stop": 265938, "strand": "-", "frame": 2, "gene": null, "product": "RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "nt": "ATGGTTGAAACAATAAATAAAATATTGAAGATTGAACGTGAATTGCAACAAGAAAATGGATATCCTCCAACAGATGAAGAAATTGCTAAAAAATATGGTGGAGATTTTACTGCTGAAAAAGTAAGATATATTAGAAAAATTAATATTGATCCAATTAGTTTGGATAAAAACATTGGAAAAGAAGAAAATTCTAGTTTTTCAGATTTTGTTAAAGACGAAAGCGTAATTAGTCCAACTTCGTATGCAAGTCATGAAGAGTTAAGTGTTATTATAAATGAAATGATTGAAGAAGGATTAAGTGATCAAGCTGATCGGGATTTAATTAGAAAACGTTTTGGAATTAACGACTTAAAGGGAAACAAATACAAACCTCATACTTTAGATGAACTTTCAAAAGAATTAGGCATTTCGAAAGAAAAAGTTAGACAATTAGAAACAAAAGTTTTGCGTAAATTGAAGCATCCTCAAAAACGTAGAAAATTGAAGGAATTCTTTAATAGCGATAGCTATGATCTAGACTAA", "aa": "MVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD", "aa_hexdigest": "bb6960937ffe99faca464418640fb8db", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0024C90298", "query_cov": 0.9884393063583815, "subject_cov": 0.8142857142857143, "identity": 0.561, "score": 166.0, "evalue": 3.83e-49, "valid": false, "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A448ZYJ5" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ5" ], "product": "RNA polymerase sigma factor" }, "genes": [], "id": "GLJLFKCOHC_486", "locus": "GCA013368035_00462" }, { "type": "cds", "sequence": "contig_1", "start": 266005, "stop": 266226, "strand": "-", "frame": 2, "gene": null, "product": "sigma-70 family RNA polymerase sigma factor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028FC70B1" ], "nt": "ATGCAAGAAGCTAAAGAAGCAAATAATTATTATAAATATAAAAAAGCTAGAGATATGTTAGTTAAAAGAAATCTTCGTTTAGTAATCAATAATGCAAAACGTTATAAAAATCGTGGATTGAATTTTATTGATTTAATATCAGAAGGAAATGCCGGAATAATGAAGGCTGTTTCAAAATATGATTACACAAAAGGATTTAAATTTTCAACATATGCTACTTGA", "aa": "MQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYAT", "aa_hexdigest": "51e3db9e296d577ba9aa1a44d51643eb", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0020416EE7", "query_cov": 0.8493150684931506, "subject_cov": 0.8157894736842105, "identity": 0.629, "score": 80.5, "evalue": 1.71e-18, "valid": false, "product": "sigma-70 family RNA polymerase sigma factor", "uniref50_id": "UniRef50_UPI0028FC70B1" }, "pscc": { "uniref50_id": "UniRef50_UPI0028FC70B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0028FC70B1" ], "product": "sigma-70 family RNA polymerase sigma factor" }, "genes": [], "id": "GLJLFKCOHC_487", "locus": "GCA013368035_00463" }, { "type": "cds", "sequence": "contig_1", "start": 266296, "stop": 267096, "strand": "-", "frame": 2, "gene": "rpoD", "product": "RNA polymerase sigma factor", "db_xrefs": [ "COG:COG0568", "COG:K", "SO:0001217", "UniRef:UniRef50_A0A501X9X8", "UniRef:UniRef90_D1J802" ], "nt": "TTGAATGAAAGAAACAGGCCAATCCAAAGACCTAATAATCAATTTGGACAGAAGGAAATAATTATGCAAGATACTAAAAATAATGTAGAATCATCAGTTAAGTGTATAACTAAAATTGTTGCAGACGAGTTAAAAAAAGTCCAAAAGAAAAATAAGGATAAAAAGGCTTTTACTCAAGATGAAATTTTTGAATTCCTAAACAAAAAGAACCTTTACGTTGATGACGAAGAAGCAGAAGCTTTACTTGAAAGCCTTTTTGCAGCTAATCTAGTCTCATCTGATACTGATGACGAAGATATTGAAGATATTGACATAAATTCGTTAGATGATGAAGTTGCTCACGCAACAACTAAAGATGATGATAATGATGACGATGACGCAGAAGAGGAAGAAAAAGATTTTGATCCTTCAAAAATTAAAGAGTTGGAAGAAGAAAATCTTGACGAAGATATAAATATTGATCAAGAATTTGACGATAGTTCAATTAGAAGCAAGGAAGAAGATGACTCAGAAATGTCTTTTGACGAAGATGATAATCATTTTGTTAAAAAGGCATTTAATGATCTTGATGATGAAGAATATAATGATTATTACGGCGAAGATTTTGACGAATATGAAGATGACGATTATCAAACAAACCACAAGCAAGGTAAAAAACAATCATACGATGACTTTGATGCTAACAATTTGCTAAAAGAATACAATGATGAAGATATAAAGTTGAGTGAAGAAAAAGTTTATGATCAAGATGATATAAAATTAACTAATAAACTAACTGAAACCAATGATATTGTTAAATGA", "aa": "MNERNRPIQRPNNQFGQKEIIMQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVK", "aa_hexdigest": "c18d5c607f951926bef9b3bab1689cef", "start_type": "TTG", "rbs_motif": null, "genes": [ "rpoD" ], "truncated": "5-prime", "pseudogene": { "start": 265993, "stop": 267033, "observations": { "mutation": [ 266004, 266007, 266286, 266298 ], "stop": [ 266004, 266007, 266286, 266298 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J802", "query_cov": 0.9360902255639098, "subject_cov": 0.45940959409594095, "identity": 0.94, "score": 372.0, "evalue": 8.44e-124, "gene_start": 22, "gene_end": 266, "reference_start": 1, "reference_end": 249, "reference_sequence": "MQDTKQDTKNNVESSVKCITKIVADELKKVQKKNKDKKAFTQDEIFEFLNKKNLYVDDEEAEALLESLFAANLVSSDTDDEDIEDIDINSLDDEVAHATTKDDDNDDDDAEEEEKDFDPSKIKELEEENLDEDINIDQEFDDSSIRSKEEDDSEMSFDEDDNHFVKKAFNDLDDEEYNDYYGEDFDEYEDDDYQTNHKQGKKQSYDDFDANNLLKEYNDEDIKLSEEKVYDQDDIKLTNKLTETNDIVKWYMRWIGKYGKLLTTNEEQALAKKMQEAKEANNYYKYKKARDMLVKRNLRLVINNAKRYKNRGLNFIDLISEGNAGIMKAVSKYDYTKGFKFSTYATWWIRQAITRAVADQARTVRVPVHMVETINKILKIERELQQENGYPPTDEEIAKKYGGDFTAEKVRYIRKINIDPISLDKNIGKEENSSFSDFVKDESVISPTSYASHEELSVIINEMIEEGLSDQADRDLIRKRFGINDLKGNKYKPHTLDELSKELGISKEKVRQLETKVLRKLKHPQKRRKLKEFFNSDSYDLD" }, "paralog": false, "identity": 0.9571428571428572, "score": 589.0, "evalue": 8.87e-212, "description": "Internal stop codon at 266004, 266007, 266286, 266298. Nonsense mutation around 266004, 266007, 266286, 266298.", "psc": { "uniref90_id": "UniRef90_D1J802", "gene": "RpoD", "product": "RNA polymerase sigma factor", "uniref50_id": "UniRef50_A0A501X9X8", "cog_id": "COG0568", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J802", "UniRef:UniRef50_A0A501X9X8", "COG:COG0568", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9X8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9X8" ], "product": "RNA polymerase sigma factor" } }, "id": "GLJLFKCOHC_488", "locus": "GCA013368035_00464" }, { "type": "cds", "sequence": "contig_1", "start": 267093, "stop": 267290, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E305FB3" ], "nt": "ATGTTTTTGTGTTTTTTAATAAACAGCATAAAAAATAATAAATCAATAGATGAAACAAATTTTGAATCATATATTACAAACAATATTGATCTTGAAGATAATGCTAAACAAATATATTTAGATTTATTCAAGGAAATTATGAGTCTTAAAAACAAAGAATATACAAAAGAAGAACTAGATAAAAATTTAAATAATTGA", "aa": "MFLCFLINSIKNNKSIDETNFESYITNNIDLEDNAKQIYLDLFKEIMSLKNKEYTKEELDKNLNN", "aa_hexdigest": "9fd6ee973b6d00dfec3f2a35ecc5442c", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E305FB3", "query_cov": 0.8, "subject_cov": 0.9454545454545454, "identity": 0.865, "score": 84.0, "evalue": 3.06e-20, "valid": false, "uniref50_id": "UniRef50_UPI001E305FB3" }, "pscc": { "uniref50_id": "UniRef50_UPI001E305FB3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E305FB3" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7769.698999999995, "isoelectric_point": 4.613018608093261 }, "id": "GLJLFKCOHC_489", "locus": "GCA013368035_00465" }, { "type": "cds", "sequence": "contig_1", "start": 267636, "stop": 268895, "strand": "-", "frame": 3, "gene": "dnaG", "product": "DNA primase", "db_xrefs": [ "COG:COG0358", "COG:L", "EC:2.7.7.101", "KEGG:K02316", "SO:0001217", "UniRef:UniRef50_D1J803", "UniRef:UniRef90_D1J803" ], "nt": "ATGGCAGAAGATAATATATTTGAAATTGTTAAACAAAAGTTAAACATTGTTGATGTTATTTCTTTATATATTTCTTTGACAAAAAAAGGAAAGAATTATGTGGCTTGTTGCCCATTTCATGGTGAGAAAACTCCTTCTTTTGTTGTGTCACAAGAAAAACAAATATTTAAATGTTTTGGTTGCTCTAAAAGCGGAAATTTAATAACTTTCATTGAACTTTATACTAAAAAAAGCCCTCTTGAAAGTATCAAGGAGCTTGTTTCAAAATTCAATTTAAATATCGATTTAACAAGGTTTAGTAATAGTATAACTACCACAACAAAAGAACAAGAAGATTTATACAATATCAATCAAATAGCGAGCGATATATTTCAATTTGAAATTTTAGAAATTAATCAAAATGAGCAATTAAAATTATTTCTTAAAAAAAGAAGGTTAACAAAACCACTTATTAAGGAATTTGAAATTGGCTTTGCTGATAAAGATAAAAATTTATGTAATATGCTAATTGCTAAAAATTGTTCATCATTTAATTTAGCCAATTCATCATTGATAGCAAGCAACAATTTTGATAACTTTTTTAATGATAGATTGATGTTTCCAATCAAAAATAAGGATGGAAGAATTGTTGCTTTTTCTGGAAGAAGTATTTATAATAATGTTGAACCAAAATATTTAAATAGTTCTGAAACAATAGTTTTTAAAAAGCATGAAGTTATGTTTAATTACTATCATGCATTGGATGAAATTATAAAGAAATCAAGTGTTTATTTAGTTGAGGGGCAATTTGATTGCATTGCTCTTTATAAAATAAACATAAAAAATGCAGTGGCACTTTTAGGCACAGCTTTATCTATACAAAATTTAGAATCTTTAAGAAATTGTAATATTGTTTTGTTTTTTGATAATGATTTTGCCGGCAAAAATGCTACTAAGAAAAATTTAAGAATAATATTAGCAAATCAAAAAAGATATAATTTAAAAGTTTCTTTTGTTTTGAATGAACATAGCAAAGATCCTGACGAATTATATAATATTGACGAAGGAAAAACATTGTCTGAGATAGCATGTTGTCCTGTTGATTTATTTATGTATTTATTCAATGAATTTAAACAAGTATTTCAATTAGAAACCAACGAATCAAGAAAGGTTGAATTAAATGCTGAATTGTTTGAATTTGTGATTTATTTGCAAGAACCATTAATATTGAAATTAAAAGAAAAATTGATTGAAAATAAAATTTTTTCAGTTGAAACTTGA", "aa": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIASDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSVET", "aa_hexdigest": "fb0e26af97cafef70a670b1bf1d716ae", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "dnaG" ], "truncated": "5-prime", "pseudogene": { "start": 267333, "stop": 268895, "observations": { "mutation": [ 267638 ], "stop": [ 267638 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J803", "query_cov": 1.0, "subject_cov": 0.6768982229402262, "identity": 0.998, "score": 811.0, "evalue": 2.23e-293, "gene_start": 1, "gene_end": 419, "reference_start": 1, "reference_end": 419, "reference_sequence": "MAEDNIFEIVKQKLNIVDVISLYISLTKKGKNYVACCPFHGEKTPSFVVSQEKQIFKCFGCSKSGNLITFIELYTKKSPLESIKELVSKFNLNIDLTRFSNSITTTTKEQEDLYNINQIANDIFQFEILEINQNEQLKLFLKKRRLTKPLIKEFEIGFADKDKNLCNMLIAKNCSSFNLANSSLIASNNFDNFFNDRLMFPIKNKDGRIVAFSGRSIYNNVEPKYLNSSETIVFKKHEVMFNYYHALDEIIKKSSVYLVEGQFDCIALYKINIKNAVALLGTALSIQNLESLRNCNIVLFFDNDFAGKNATKKNLRIILANQKRYNLKVSFVLNEHSKDPDELYNIDEGKTLSEIACCPVDLFMYLFNEFKQVFQLETNESRKVELNAELFEFVIYLQEPLILKLKEKLIENKIFSVETWNKYLFLYGKENFPLHPISKNNLKPSLDNEYNKKNYSKNSNYKKYEDLNFAYSDPSFDRLDFFEEPLIQQPKLITGRNLVFYLIIKTIIKHPQYIANYQLVDFARMAFSNGCQNHKMFLCFLINSIKNNKSIDETNFESYITNNIDLEDNTKQIYLDLFKEIMSLKNKEYTKEELDKNLNNWMKETGQSKDLIINLDRRK" }, "paralog": false, "identity": 0.9961538461538462, "score": 1032.0, "evalue": 0.0, "description": "Internal stop codon at 267638. Nonsense mutation around 267638.", "psc": { "uniref90_id": "UniRef90_D1J803", "gene": "DnaG", "product": "DNA primase", "ec_ids": [ "2.7.7.101" ], "uniref50_id": "UniRef50_D1J803", "cog_id": "COG0358", "cog_category": "L", "kegg_orthology_id": "K02316", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J803", "EC:2.7.7.101", "UniRef:UniRef50_D1J803", "COG:COG0358", "COG:L", "KEGG:K02316" ] }, "pscc": { "uniref50_id": "UniRef50_D1J803", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J803" ], "product": "DNA primase" } }, "id": "GLJLFKCOHC_490", "locus": "GCA013368035_00466" }, { "type": "cds", "sequence": "contig_1", "start": 268906, "stop": 269424, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "TTGCTTGGTATTGCAAATCGTGGCAATTATGATTTAAGCCAACATATGAAATATTCAAATGAATCTCTTGAATATTTGTTAGACGATGGAACTAAAATAATTCCTCATGTTATTGAACCTTCAATTGGTTTAGATCGTTTATTGCTTGCATTGCTTGTAGATTCATATGAAGAACAAGAACTAGAAAATAATGATGTAAGAATTGTTTTAAAACTTGATTTCAATATTGCACCATATCAAGTTGCGATTTTTCCGTTAATGAAAAAACTTAACTCTAAGGCTAAAGAAATATTTGATAATTTATTACAAAAAACTGAATTAAGATTAATTTATGATGAAAGCGGTTCGATAGGAAAACGTTACCGTCGTCAAGATGCAATTGGTACACCATTTGCAGTAACAATAGATTTTGACACATTAGAAAAAAATACAGTAACTGTCAGAGAACGCGATTCTATGAAACAACATATAGTTGAATTAGACAACTTAATTAAGTTTTTTGAAAATTATAAAAAATAA", "aa": "MLGIANRGNYDLSQHMKYSNESLEYLLDDGTKIIPHVIEPSIGLDRLLLALLVDSYEEQELENNDVRIVLKLDFNIAPYQVAIFPLMKKLNSKAKEIFDNLLQKTELRLIYDESGSIGKRYRRQDAIGTPFAVTIDFDTLEKNTVTVRERDSMKQHIVELDNLIKFFENYKK", "aa_hexdigest": "8c263d24e9f43520b56f3d253003f57f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A3B0P9T9", "query_cov": 0.9651162790697675, "subject_cov": 0.9707602339181286, "identity": 0.6629999999999999, "score": 228.0, "evalue": 5.43e-74, "valid": false, "product": "Glycine--tRNA ligase", "uniref50_id": "UniRef50_A0A3B0P9T9" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 93, "aa_cov": 0.5232558139534884, "hmm_cov": 1.0, "evalue": 2.1496869263663766e-23, "score": 77.72394561767578, "start": 80, "stop": 169 } ], "seq_stats": { "molecular_weight": 19998.697499999995, "isoelectric_point": 5.293097114562988 }, "id": "GLJLFKCOHC_491", "locus": "GCA013368035_00467" }, { "type": "cds", "sequence": "contig_1", "start": 269569, "stop": 269835, "strand": "-", "frame": 2, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "nt": "ATGTTTAAAACCCAAATGGGTGCAATTGATACTGCGGCAAATCAAACATATTTGCGTCCTGAAACAGCCCAAGGAATTTTTATAAATTTTAAAAATGTCTTAAGAACAACTAGAGCTAAGTTACCTTTTGGAATTGGCCAAATTGGTAAAAGTTTTAGAAATGAAATTACACCTAGGGATTTTATTTTTAGAACAAGGGAATTTGAACAAATGGAATTAGAATATTTCTGCAATCCTAGTGAAGATGAAAAGTTTTACCATTATTGA", "aa": "MFKTQMGAIDTAANQTYLRPETAQGIFINFKNVLRTTRAKLPFGIGQIGKSFRNEITPRDFIFRTREFEQMELEYFCNPSEDEKFYHY", "aa_hexdigest": "fb21550e42e24642fc3daa802e4e2712", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI003241E146", "query_cov": 0.9318181818181818, "subject_cov": 0.8913043478260869, "identity": 0.695, "score": 123.0, "evalue": 5.97e-35, "valid": false, "product": "aminoacyl--tRNA ligase-related protein", "uniref50_id": "UniRef50_X1KVU4" }, "pscc": { "uniref50_id": "UniRef50_X1KVU4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_X1KVU4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_492", "locus": "GCA013368035_00468" }, { "type": "cds", "sequence": "contig_1", "start": 270124, "stop": 270282, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAGATAAAATACAAAAAAATATTCAAAATATAATAAATCACTTAAAAGTTTCTGGTTTTGTTTATCAAAATTCAGAAATCTATGGAGGCTTAATAAACACCTGGGATTATGGGCCACTAGCAGTTAATATTATGACTGCGATTAAGAAATATTGA", "aa": "MGDKIQKNIQNIINHLKVSGFVYQNSEIYGGLINTWDYGPLAVNIMTAIKKY", "aa_hexdigest": "060c8f592c19fc45fda4a41c098dc6d2", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5915.793100000001, "isoelectric_point": 9.10114688873291 }, "id": "GLJLFKCOHC_493", "locus": "GCA013368035_00469" }, { "type": "cds", "sequence": "contig_1", "start": 270427, "stop": 271755, "strand": "+", "frame": 1, "gene": "ffh", "product": "signal recognition particle protein", "db_xrefs": [ "COG:COG0541", "COG:U", "EC:3.6.5.4", "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501", "KEGG:K03106", "RefSeq:WP_012855495.1", "SO:0001217", "UniParc:UPI0001BC21DF", "UniRef:UniRef100_D1J805", "UniRef:UniRef50_A0A448ZXZ7", "UniRef:UniRef90_D1J805" ], "nt": "ATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAGTTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCGAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAGAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCTGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACAGATTTCTTTACTATGGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAAGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATCACAAAATTAGATAGTGATGCTCGTGGTGGCGCAGCATTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATGGGCGATGTTCTAACATTAATTGAAAAAGCCTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTAATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCCGGAATGGCAGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTTGAGAAAAAAAACCCGCGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAAGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAGCAAATGAAAGAATTAGCAAACGGAAATCTAAAATTAAAAAATGGCTTGGATGAATAA", "aa": "MLDFIQKRMQNSIQKINKKMSINEEDILEILREIKISLLEADVNLEVVKNFTKEVKAKALSSQIIGKLNQQQTVLKIFKDELTRILGSKSIDIKTNNNPTKIMMVGLQGSGKTTTAAKLSVYFKKKNLAVNPLLVGDDIYRPAAREQLQQLAKQTQTDFFTMEENNAINIAQKAIELAKENKNDLVIIDTAGRLAIDENLMQELKDIKKYTHPDYIFLVVDAMSGQDVINTAKIFHQELNLDGTIITKLDSDARGGAALSITHLLNVPIIFIGTGEKLTGLELFHPDRMADRILGMGDVLTLIEKASEEVDEKMMKKIGYKMLSGQFNLMDLMNSLAQIKKLGKMKAILRMIPGMADKVSDEKIEEAENKFRIYTYLINSMTNFEKKNPRVLKNPSRKQRILQGSGRSPREFNMLISDFERMSKQMKELANGNLKLKNGLDE", "aa_hexdigest": "5f3bd5f4930eef166ea57925f9607a43", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC21DF", "ncbi_nrp_id": "WP_012855495.1", "uniref100_id": "UniRef100_D1J805", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21DF", "RefSeq:WP_012855495.1", "UniRef:UniRef100_D1J805" ] }, "ips": { "uniref100_id": "UniRef100_D1J805", "uniref90_id": "UniRef90_D1J805" }, "psc": { "uniref90_id": "UniRef90_D1J805", "gene": "ffh", "product": "signal recognition particle protein", "ec_ids": [ "3.6.5.4" ], "uniref50_id": "UniRef50_A0A448ZXZ7", "cog_id": "COG0541", "cog_category": "U", "kegg_orthology_id": "K03106", "go_ids": [ "GO:0003924", "GO:0005048", "GO:0005525", "GO:0009306", "GO:0048501" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXZ7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXZ7" ], "product": "Signal recognition particle protein" }, "genes": [ "ffh" ], "id": "GLJLFKCOHC_494", "locus": "GCA013368035_00470" }, { "type": "cds", "sequence": "contig_1", "start": 271875, "stop": 272210, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAACAATCAATTTCATTTTAAATACTTATTTTTCAAAACAAAATTGTCAAGAATTAAAACCTCGCGATAAAAAAATAGCCTCTGAAAATATCAAATATATTTTAGAAACATTTAAAAATCATTCATATTTTTTAAATAATTTATTTAATCACTTCATCAATAAAATTAAAACTATTTCTCCATATAACCTAATTGTAAAAACTAAAAAATTAAATTCAATATTTAAATTAAGAAATATTATTATTTTTGATAGATATGTTTTATCAAGTCTAAAAATATCAAAAGCAATATTAGTCTTAATTTCAATTAAAAATAAGAATAAATTATAA", "aa": "MQETINFILNTYFSKQNCQELKPRDKKIASENIKYILETFKNHSYFLNNLFNHFINKIKTISPYNLIVKTKKLNSIFKLRNIIIFDRYVLSSLKISKAILVLISIKNKNKL", "aa_hexdigest": "ec6edd12996e898096a0190c5c3803b2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13213.552599999974, "isoelectric_point": 10.082484626770018 }, "id": "GLJLFKCOHC_495", "locus": "GCA013368035_00471" }, { "type": "cds", "sequence": "contig_1", "start": 272292, "stop": 272450, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTTATTTTCATTCAACGATAATCAAAAATATATAGAAATTAAGAAATTTTTGTCTCAATTGAATACATTTAAGGAATTTATTTATTTTATAGTAGAAACACTTTTAAACTATAGCGATATTTATGCAAAATTAAAAACCTTTGATGAATTATGA", "aa": "MFLFSFNDNQKYIEIKKFLSQLNTFKEFIYFIVETLLNYSDIYAKLKTFDEL", "aa_hexdigest": "bf67931aa5b4ac998666b296d549052f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6415.358999999999, "isoelectric_point": 5.0959794998168935 }, "id": "GLJLFKCOHC_496", "locus": "GCA013368035_00472" }, { "type": "cds", "sequence": "contig_1", "start": 272595, "stop": 272759, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGATGCATTTTTAAGCAACGAAACAAATAGCAAAGCTATATTTTTAACGTTATTTAAAGATGGAAAAATTGATGATATATTATTCAGATTACAATCAAGACTTGAAGATGTATTATTAACCAAAAAAACAATTATTAATATTGATTTGCGTGAACAATGA", "aa": "MIDAFLSNETNSKAIFLTLFKDGKIDDILFRLQSRLEDVLLTKKTIINIDLREQ", "aa_hexdigest": "17ab41e01f210c6116e5c816bfd4f287", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6298.264899999998, "isoelectric_point": 5.125876808166503 }, "id": "GLJLFKCOHC_497", "locus": "GCA013368035_00473" }, { "type": "cds", "sequence": "contig_1", "start": 273195, "stop": 273548, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATTTTATAAATTAACTAATTTAATAACAGCTAAAAATGAAACAAAAACCAATTTAATTATTGAAATAAAAAAAGATTTTGAAAATATCGGCAAAGAAATTATTTTAAAATACCAAAAGCTTGTTGAATATATTCAAAAAATTAATCCACTAGCAAATATCGTGGTAACAAATTATGCCAAACCATTTATGGCCTTGCAAGATTTAATTAATTCGATTTACATTTCTTCTTCATATAACAATGAATTTGATTTGGTTGAAATGATACAAGATTTATTAAACAATGTCGCTAAAGAAGTAGCTAAAAAATGCCAATGTTTATATGTAGATATTTATGAAGAAAAATATTGA", "aa": "MPFYKLTNLITAKNETKTNLIIEIKKDFENIGKEIILKYQKLVEYIQKINPLANIVVTNYAKPFMALQDLINSIYISSSYNNEFDLVEMIQDLLNNVAKEVAKKCQCLYVDIYEEKY", "aa_hexdigest": "2f36732b322702c4ee700727d3c030d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13723.87469999999, "isoelectric_point": 5.486008644104003 }, "id": "GLJLFKCOHC_498", "locus": "GCA013368035_00474" }, { "type": "cds", "sequence": "contig_1", "start": 273650, "stop": 274009, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_241863330.1", "SO:0001217", "UniParc:UPI001F5209ED", "UniRef:UniRef100_UPI001F5209ED", "UniRef:UniRef50_UPI001F5209ED", "UniRef:UniRef90_UPI001F5209ED" ], "nt": "ATGGCTAAAAATCAAAAATACAATTTAAATGACGAAATTAAATATGTTGCTTTAGGAGATTCATTTGCAGCGGGATTTAATAGCAAAGTAGGGTTTAATACTAATGGAAAATTATCAAATGGTAATATAGATGGTTTGGGTTATCCATCATTTTTAGCCTTAAAATTGCGAGATTTAAACTATAGATTAACAAGTTTTTACAATCTAGCAATACCAGCGGGTAATTTAGACATAATTTATGCCTTAACTTCAAATAATGAAAATGAATTAATTGCAAATTCAAAAAGTCTTGATTTGCTTCAATCAATTGATTGGCATGTTTCAAATCCTTCAAAGAATTTTTTTGATGAATTTTTTTAA", "aa": "MAKNQKYNLNDEIKYVALGDSFAAGFNSKVGFNTNGKLSNGNIDGLGYPSFLALKLRDLNYRLTSFYNLAIPAGNLDIIYALTSNNENELIANSKSLDLLQSIDWHVSNPSKNFFDEFF", "aa_hexdigest": "dc1da0cfec7f76afb539baa8cced0a54", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI001F5209ED", "ncbi_nrp_id": "WP_241863330.1", "uniref100_id": "UniRef100_UPI001F5209ED", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F5209ED", "RefSeq:WP_241863330.1", "UniRef:UniRef100_UPI001F5209ED" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F5209ED", "uniref90_id": "UniRef90_UPI001F5209ED" }, "psc": { "uniref90_id": "UniRef90_UPI001F5209ED", "uniref50_id": "UniRef50_UPI001F5209ED" }, "pscc": { "uniref50_id": "UniRef50_UPI001F5209ED", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F5209ED" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13295.708399999981, "isoelectric_point": 5.198744392395019 }, "id": "GLJLFKCOHC_499", "locus": "GCA013368035_00475" }, { "type": "cds", "sequence": "contig_1", "start": 274119, "stop": 274334, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAAAACTAAAAGATGAAGAAACAGGTGCCAAATTAGCTGTAGTTAGTTCAATAAAGTTGGAAGCTTTAAATATAAATGAAGTTATTGTTGCTACTTACCGCCTAATATCTAAGTATTATCCTCAAAAAATTATTGATATAAAATTGCAACCAAGTCAAGATGAAGAAGAAATCTTTAGCCAATTGAGTTTATATGCTAAAAATAATAATTGA", "aa": "MPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLSLYAKNNN", "aa_hexdigest": "8cd3b9a7b842bd0dcfd511df535b60af", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8098.235599999996, "isoelectric_point": 5.311001396179199 }, "id": "GLJLFKCOHC_500", "locus": "GCA013368035_00476" }, { "type": "cds", "sequence": "contig_1", "start": 274380, "stop": 274829, "strand": "-", "frame": 3, "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "db_xrefs": [ "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "SO:0001217", "UniRef:UniRef50_A0A077L649", "UniRef:UniRef90_A0A454CAF5" ], "nt": "ATGGCCAAAACCATTAGATTATTAAATGAAAAAATAAGATTATTTGATTTATTTATAATTGTTTTAGATGCACGCTTACCTCTTACTTCGTTTAATAACGATATTTATAAGATGGCTAATAATAAGCCAATTTTATTTATATTAAACAAAAGCGACAAAGCAGATCTATCTAAATTAAAACCTTTTATAAATGAATATGAAAAGAAAGGTGTTGTTGTAACAACTAATTTGAAAGATAAATTAGCCTATAAAAAAATAAATTCAGCTCTTAATAATATTTATTTAAAAGTAAAAGAAAAAAATGCACAGAAACATAAGCTAACACCTGCATTAAAATGTGTCGTGCTTGGCGTTCCTAATGTTGGTAAAAGTACCTTAATAAATTTATTGGCTGGTTCTAAAGTTGCAAAAGTGGGTGCAATGGCTGGAGTAACAAAATCAGAACAATGA", "aa": "MAKTIRLLNEKIRLFDLFIIVLDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQ", "aa_hexdigest": "5210e832a85ee2d2043f177ad2b5f1f3", "start_type": "ATG", "rbs_motif": null, "genes": [ "ylqF" ], "truncated": "both", "pseudogene": { "start": 274077, "stop": 274853, "observations": { "mutation": [ 274121, 274382, 274844 ], "stop": [ 274121, 274382, 274844 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CAF5", "query_cov": 1.0, "subject_cov": 0.5173611111111112, "identity": 0.993, "score": 278.0, "evalue": 2.49e-92, "gene_start": 1, "gene_end": 149, "reference_start": 9, "reference_end": 157, "reference_sequence": "MFNWFPGHMAKTIRLLNEKIRLFDLFIIILDARLPLTSFNNDIYKMANNKPILFILNKSDKADLSKLKPFINEYEKKGVVVTTNLKDKLAYKKINSALNNIYLKVKEKNAQKHKLTPALKCVVLGVPNVGKSTLINLLAGSKVAKVGAMAGVTKSEQWINCKNYLLLDTPGLLMPKLKDEETGAKLAVVSSIKLEALNINEVIVATYRLISKYYPQKIIDIKLQPSQDEEEIFSQLSLYAKNNNWILKNQLPDIKKAIVQLISYFNNLNNIVYDQCQEKEQNGWKSKI" }, "paralog": false, "identity": 0.9844961240310077, "score": 490.0, "evalue": 1.9e-178, "description": "Internal stop codon at 274121, 274382, 274844. Nonsense mutation around 274121, 274382, 274844.", "psc": { "uniref90_id": "UniRef90_A0A454CAF5", "gene": "ylqF", "product": "ribosome biogenesis GTPase YlqF", "uniref50_id": "UniRef50_A0A077L649", "cog_id": "COG1161", "cog_category": "J", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CAF5", "UniRef:UniRef50_A0A077L649", "COG:COG1161", "COG:J", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L649", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L649" ], "product": "Ribosome biogenesis GTPase A" } }, "id": "GLJLFKCOHC_501", "locus": "GCA013368035_00477" }, { "type": "cds", "sequence": "contig_1", "start": 274862, "stop": 275077, "strand": "-", "frame": 1, "gene": "trmH", "product": "TrmH family RNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "nt": "ATGTTTGGAACTGAAAGCACAGGAATTCCTAAGAGAATTATGCAAACAAATATTCCTAATTGTTTGCGTATCCCAATGTTTGATGAATGTCGTAGTTTAAATTTAGCCAATAGTGTTGCAATAGTACTATTTGAAGTATTAAGACAAAATGATTTCGCTGGACTATCAAAATTTGAAAGTCAAAAAGGGAAGGATTTTATTTTAAGGAAAGATTGA", "aa": "MFGTESTGIPKRIMQTNIPNCLRIPMFDECRSLNLANSVAIVLFEVLRQNDFAGLSKFESQKGKDFILRKD", "aa_hexdigest": "6bd8369e0e35ce00d282458f1ad0d93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH15", "query_cov": 1.0140845070422535, "subject_cov": 0.8674698795180723, "identity": 0.514, "score": 79.0, "evalue": 7.73e-18, "valid": false, "product": "TrmH family RNA methyltransferase", "uniref50_id": "UniRef50_A0A449AH15" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH15", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH15" ], "product": "TrmH family RNA methyltransferase" }, "genes": [ "trmH" ], "id": "GLJLFKCOHC_502", "locus": "GCA013368035_00478" }, { "type": "cds", "sequence": "contig_1", "start": 275081, "stop": 275260, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAAGGCTGCTGGGCACTTTTTAACTGAAATTCAACATGAAGTTCATGAAAATTATGAAGAATTTTTTAAAAAATATGGTGATAAAAATATTTATTATGTAACAAGATACGGTTTAAAAAATTACGTAGATGTTGATTACAAACAAGAATTGCAAACAAATGGTGAACTATGA", "aa": "MKRKAAGHFLTEIQHEVHENYEEFFKKYGDKNIYYVTRYGLKNYVDVDYKQELQTNGEL", "aa_hexdigest": "b02bb34b0d68b2a9b578926e87dc7e26", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7192.941000000004, "isoelectric_point": 6.26146297454834 }, "id": "GLJLFKCOHC_503", "locus": "GCA013368035_00479" }, { "type": "cds", "sequence": "contig_1", "start": 275654, "stop": 275920, "strand": "-", "frame": 1, "gene": "rpsO", "product": "30S ribosomal protein S15", "db_xrefs": [ "COG:COG0184", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855499.1", "SO:0001217", "UniParc:UPI0001BC21E3", "UniRef:UniRef100_D1J809", "UniRef:UniRef50_P0C0K0", "UniRef:UniRef90_D1J809" ], "nt": "ATGGTATCAAAAGAAAAAAAAGCTGAATTAGTTGTTAAATTCGGACGTAATGCAAAAGACACTGGATATTTACCAGTACAAATAGCAATTTTAACTGAAGACATTGAAAGTTTAAAAGAACATTTTGCAGTAAATAAAAAAGATTTACATTCAATGAGAGGATTCATTGCTAAAGTTAATCACCGTAAAGCATTACTAAATCACTTAAAAGACGAAAACTACAACTTATATTTAGAAACCATCAAAGCTTTAAACATCAGAAAATAA", "aa": "MVSKEKKAELVVKFGRNAKDTGYLPVQIAILTEDIESLKEHFAVNKKDLHSMRGFIAKVNHRKALLNHLKDENYNLYLETIKALNIRK", "aa_hexdigest": "fbc082619cd1a86501261f37c1edf520", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC21E3", "ncbi_nrp_id": "WP_012855499.1", "uniref100_id": "UniRef100_D1J809", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E3", "RefSeq:WP_012855499.1", "UniRef:UniRef100_D1J809" ] }, "ips": { "uniref100_id": "UniRef100_D1J809", "uniref90_id": "UniRef90_D1J809" }, "psc": { "uniref90_id": "UniRef90_D1J809", "gene": "rpsO", "product": "30S ribosomal protein S15", "uniref50_id": "UniRef50_P0C0K0", "cog_id": "COG0184", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P0C0K0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P0C0K0" ], "product": "Small ribosomal subunit protein uS15" }, "genes": [ "rpsO" ], "id": "GLJLFKCOHC_504", "locus": "GCA013368035_00480" }, { "type": "tRNA", "sequence": "contig_1", "start": 276043, "stop": 276119, "strand": "-", "gene": "trnI", "product": "tRNA-Ile(gat)", "amino_acid": "Ile", "anti_codon": "gat", "score": 83.1, "nt": "GCGAGCGTAGCTCAGTTGGTTAGAGCACACGACTGATAATCGTGAGGTCGATGGTTCAAGTCCATTCGTTCGCACCA", "db_xrefs": [ "SO:0000263" ], "anti_codon_pos": [ 276083, 276085 ], "id": "GLJLFKCOHC_31", "locus": "GCA013368035_00481" }, { "type": "cds", "sequence": "contig_1", "start": 276168, "stop": 276566, "strand": "-", "frame": 3, "gene": "rpsI", "product": "30S ribosomal protein S9", "db_xrefs": [ "COG:COG0103", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02996", "RefSeq:WP_012855500.1", "SO:0001217", "UniParc:UPI0001BC21E4", "UniRef:UniRef100_D1J810", "UniRef:UniRef50_Q98Q72", "UniRef:UniRef90_D1J810" ], "nt": "ATGGCAGATAAAGTAAGATACTATGGTTTAGGTCGTCGTAAATCTTCAGTAGCAAGAGTTTATATTTTACCAGGTAGTGGAAATTTAATTATTAATGGAATAGTTGCAAAAGAATACTTAAATTCAGAAATTTTAATAAAAGATGCTTTAAGTCCTTTTGCAGTAACTGAAACTGCTAACCAATTTGATGTTTTTGCAAATGTTAATGGTGGTGGTTTAACAGGACAAGCTGGAGCAATTAGATTAGGTATTGCTAGAGCATTATTAGAAGCATCTAATAACGAATATAGAAATAAATTAAAAGATGCAGGATTTTTAACACGTGATGCTAGAGTTAAAGAACGTAAGAAATTCGGTTTAAGAAAAGCAAGACGTGCAAGACAATTTTCAAAACGTTAA", "aa": "MADKVRYYGLGRRKSSVARVYILPGSGNLIINGIVAKEYLNSEILIKDALSPFAVTETANQFDVFANVNGGGLTGQAGAIRLGIARALLEASNNEYRNKLKDAGFLTRDARVKERKKFGLRKARRARQFSKR", "aa_hexdigest": "8a2d74830272a058243860cc79a3c920", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC21E4", "ncbi_nrp_id": "WP_012855500.1", "uniref100_id": "UniRef100_D1J810", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC21E4", "RefSeq:WP_012855500.1", "UniRef:UniRef100_D1J810" ] }, "ips": { "uniref100_id": "UniRef100_D1J810", "uniref90_id": "UniRef90_D1J810" }, "psc": { "uniref90_id": "UniRef90_D1J810", "gene": "rpsI", "product": "30S ribosomal protein S9", "uniref50_id": "UniRef50_Q98Q72", "cog_id": "COG0103", "cog_category": "J", "kegg_orthology_id": "K02996", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q72", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q72" ], "product": "Small ribosomal subunit protein uS9" }, "genes": [ "rpsI" ], "id": "GLJLFKCOHC_505", "locus": "GCA013368035_00482" }, { "type": "cds", "sequence": "contig_1", "start": 276569, "stop": 276853, "strand": "-", "frame": 1, "gene": null, "product": "50S ribosomal protein L13", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "nt": "ATGGGCGACAATGTGGTTGTGATCAATGCTAAAGATGTACTTTTAACTGCTAAAAAAGATGAAATAAAAGTATATTACCACCACACTGGATACCCAGGTGGATTAAAACAAATTACCGCTGCTGATTTAAGAGCAAAAAAACCAGAATTATTAGTTGAAAAAGCTGTTAGAGGAATGCTTCCACACACCAAATTAGGTCGTAAACAATTTAAAAATTTATATGTTTATGCAGGTTCTGAACACAAGCAAGAAGCACAACAACCAGTTAAATTAGAGGTTAAATAA", "aa": "MGDNVVVINAKDVLLTAKKDEIKVYYHHTGYPGGLKQITAADLRAKKPELLVEKAVRGMLPHTKLGRKQFKNLYVYAGSEHKQEAQQPVKLEVK", "aa_hexdigest": "5376e3427d2b3b0ced82ebaa8fcaf930", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P644", "query_cov": 1.0, "subject_cov": 0.831858407079646, "identity": 0.649, "score": 124.0, "evalue": 3.48e-35, "valid": false, "gene": "rplM", "product": "50S ribosomal protein L13", "uniref50_id": "UniRef50_A0A3B0P644", "kegg_orthology_id": "K02871", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P644", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P644" ], "product": "50S ribosomal protein L13" }, "genes": [], "id": "GLJLFKCOHC_506", "locus": "GCA013368035_00483" }, { "type": "cds", "sequence": "contig_1", "start": 277116, "stop": 277778, "strand": "-", "frame": 3, "gene": "rsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "db_xrefs": [ "COG:COG1385", "COG:J", "EC:2.1.1.193", "RefSeq:WP_175949845.1", "SO:0001217", "UniParc:UPI001588ECE8", "UniRef:UniRef100_UPI001588ECE8", "UniRef:UniRef50_A0A448ZY27", "UniRef:UniRef90_D1J812" ], "nt": "ATGTATAAATTTTTTGCCAACAAATTAGAAGATGGAATTTTTTATTTTAGTGATGAAATCCAAAAGCATTTTAAGGTTTTACGTTTTAACAAAGAAAATATTATTGTAAATTATGAAAATAAGTTTTATGAATGTCAATTTGTTTTTCCTAATAGTGCTAAATTAGTTAAAGAGTTAGATATTAATAATGAATTGGATTATAAAATTTCAGTTGCAATTCCTTTAATTAAACAAAACCATTTTGAAATAGCATTGCAAAAAGCAGTTGAACTTGGCGCTTATGAAATTTATGTATTTCAAAGCCAATATGTTGATAAATCTAATTTAAATTTTGATTCCAAAAAGCAACGTTATGAAAAAATAATTTTTGAAGCTTGCCAGCAAAGTTTTAGAAATTATGTCCCAATTCTTCATGATCCAATTTCATTTTCAGAACTTTGTGTCTTAGATATTAAAAATAAAGTATTAGCTTATGAAAATAAAAAGAATAACGAATTAATTAAAGATTGCCAAGATGCTTTAATTATTGTTGGCCCTGAAGGTGGATTTAGCCAACAAGAAATTGATTATGCTATATCTTGCAATGTTCAAGTTGTTTCATTAACAAAAACAATTTTAAGGGCTGAGACCGCCTTAATTTACATGCTTTCAAAATTAAATTAA", "aa": "MYKFFANKLEDGIFYFSDEIQKHFKVLRFNKENIIVNYENKFYECQFVFPNSAKLVKELDINNELDYKISVAIPLIKQNHFEIALQKAVELGAYEIYVFQSQYVDKSNLNFDSKKQRYEKIIFEACQQSFRNYVPILHDPISFSELCVLDIKNKVLAYENKKNNELIKDCQDALIIVGPEGGFSQQEIDYAISCNVQVVSLTKTILRAETALIYMLSKLN", "aa_hexdigest": "c4fd54c3617a74f57bdaf63ca873d432", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI001588ECE8", "ncbi_nrp_id": "WP_175949845.1", "uniref100_id": "UniRef100_UPI001588ECE8", "db_xrefs": [ "SO:0001217", "UniParc:UPI001588ECE8", "RefSeq:WP_175949845.1", "UniRef:UniRef100_UPI001588ECE8" ] }, "ips": { "uniref100_id": "UniRef100_UPI001588ECE8", "uniref90_id": "UniRef90_D1J812" }, "psc": { "uniref90_id": "UniRef90_D1J812", "gene": "RsmE", "product": "16S rRNA (uracil(1498)-N(3))-methyltransferase", "ec_ids": [ "2.1.1.193" ], "uniref50_id": "UniRef50_A0A448ZY27", "cog_id": "COG1385", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY27", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY27" ], "product": "Ribosomal RNA small subunit methyltransferase E" }, "genes": [ "rsmE" ], "id": "GLJLFKCOHC_507", "locus": "GCA013368035_00484" }, { "type": "cds", "sequence": "contig_1", "start": 278172, "stop": 278399, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATTTCTTTTACTGAGCTTGGAATAGTAACTTTTGTGGTTTTTTTATCCGAATATGTTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCCACTAGATAATGCGCCTTCTCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTTAATACTTTTATTTTGA", "aa": "MPISFTELGIVTFVVFLSEYVNIFLLFLSIKKLSLISKFLLFELIKILEIFPLDNAPSPISFTEPGIVIDLILLF", "aa_hexdigest": "cb64b853f0fbccc92304717d6a7e8911", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8535.28119999999, "isoelectric_point": 4.4943956375122065 }, "id": "GLJLFKCOHC_508", "locus": "GCA013368035_00485" }, { "type": "cds", "sequence": "contig_1", "start": 278727, "stop": 278939, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAACCAGAGAACGCACTTTCGCCAATTTCTTTTACTGACCGCGGTATAACAATATATCCAATGTTTGTATTAAAAAATGCCTTAGCGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATGTTTTTGCAACCAGAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAA", "aa": "MQPENALSPISFTDRGITIYPMFVLKNALASIFSRPSFKITSLMFLQPENALSPISFTEPGIVIDSIFVS", "aa_hexdigest": "03101ea0558bee6214e1c9076e4e3369", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7737.958299999996, "isoelectric_point": 4.93575038909912 }, "id": "GLJLFKCOHC_509", "locus": "GCA013368035_00486" }, { "type": "cds", "sequence": "contig_1", "start": 280086, "stop": 280217, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGTGGCAATAAAGAATTTATGTTAATGAAGCAAGAGGCTAAATCAAACAATGATTATAGCCTTGTGGATGCATTTAAAATAAATTTAAATTGGAAGAAAAATTATAATAAAAAATCTATGACAAATTAA", "aa": "MGGNKEFMLMKQEAKSNNDYSLVDAFKINLNWKKNYNKKSMTN", "aa_hexdigest": "9e9e4dc1c25a3dec40fc23c5853da432", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5074.790000000002, "isoelectric_point": 9.632495307922362 }, "id": "GLJLFKCOHC_510", "locus": "GCA013368035_00487" }, { "type": "cds", "sequence": "contig_1", "start": 280573, "stop": 281082, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13306.12" ], "nt": "TTGGCATTTGATTTTTCAATAATTTCGCATATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTCTTCATTGATAAAAATAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCTAGTTCGGTAAAAGAAATTGGCGAAAGTGCGTTTTCTGATTGCAAAAACCTTGAAGAAGTAAGTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTCAAATACAAACATTGAATCTATTATTATTCCAGATTCAGTAAAAGAAATTGGCCAAAGAGCGTTTTCTAGTTGCATAAACCTTGAAGAAGTAAGTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGAGGCATTTTCAAATACAAACATTGAATCTATTATTATTCCAGATTCAGTAAAAGAAATTGGCCGAAGAGCGTTTTCTAATTGCAAAAACCTTAAAGAAGTAACTTATAATGGTGATGCAAGTAAAATAAATTGA", "aa": "MAFDFSIISHISINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPSSVKEIGESAFSDCKNLEEVSLNEGLEKIGAEAFSNTNIESIIIPDSVKEIGQRAFSSCINLEEVSLNEGLEKIGAEAFSNTNIESIIIPDSVKEIGRRAFSNCKNLKEVTYNGDASKIN", "aa_hexdigest": "ad7e5e4dc812ba7e9b9f9380be38668d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13306.12", "name": "LRR_5", "length": 119, "aa_cov": 0.7041420118343196, "hmm_cov": 1.0, "evalue": 5.6218471538726686e-42, "score": 138.06675720214844, "start": 39, "stop": 157 } ], "seq_stats": { "molecular_weight": 18703.84430000001, "isoelectric_point": 4.967011833190917 }, "id": "GLJLFKCOHC_511", "locus": "GCA013368035_00488" }, { "type": "cds", "sequence": "contig_1", "start": 281092, "stop": 282366, "strand": "-", "frame": 2, "gene": null, "product": "Leucine-rich repeat protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9V9", "UniRef:UniRef90_A0A454C9V9" ], "nt": "TTGGGTTGTAGAAACCTTGAAGAAGTAATTTTAAATGAAGGTCTTGAAAAGATAGGCGTTAGGGCATTTTTAAATACAAAAATTGAATCTATTACTATTCCTGATTCAGTAAAAGAAATAGGCAGTTTAGCATTTAATTTTTCAAAAATTTCACATATTTCAATTAATTCAAATAATAAAAATTTAGAAATTAAAGATAACTTTCTTATTGATAAAAATAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGACGAAAGTGCGTTTTTTGGTTGTAGAAACCTTAAAGAAGTGATTTTAAATGAAGGTCTTGAAAAAATTAGAGCCGGGGCATTTTTAAATACAAAAATTGAATCTATTACTATTCCTGATTCAGTAAAAGAAATAGGCAGTTTAGCATTTAATTTTTCAAAAATTTCACATATTTCAATTAATTCAAATAATAAAAATTTAGAAATTAAAGATAATTTTCTTATTGATAAAAATAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGGTAAAGGTGCGTTTTATGGTTGCAGAAACCTTGAAGAAGTAATTTTAAATGAAGGTCTTGAAAAGATAGGCGTTAGGGCATTTTCAAGTCCAAGAATTAAATCTATTAATATTCCCGGTTCAGTAAAAGAAATTGGCGAAGGTGCATTATCTATTTGGGATATTCGAAGTATTTCAATTAATTCAAATAATAAAAATTTTGAAATTAAAGATAACTTCTTCATTGATAAAAATAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCTAGTTCAATAAAAGAAATTGGCGAAGATGCGTTTTATGGTTGCGGAAACCTTGAAGAAGTAATTTTAAATGAAGGTCTTGAAAAAATTGGCGCTGCGGCATTTCGAACTATAAAAATTGAATCTATTACTATTCCAAGCTCGTTAAAAGAAATTGGCTATTTGGCATTTGATTTTTCAAAAATTTCACATATTTCAATTAATTCATATAATAAAAATTTTAAAATTAAAGATAACTTTTTTATTGATAAAAAAAATAAAAAAATATTAGCATATTTGGATAAAAAAACCACAAAAGTTACTATTCCTCGTTCAATAAAAGAAATTGGTAAAGGAGCGTTTTCTGATTGCAAAAACCTTGAAGAATTAATTTTAAATGAAGGTCTTGAATGA", "aa": "MGCRNLEEVILNEGLEKIGVRAFLNTKIESITIPDSVKEIGSLAFNFSKISHISINSNNKNLEIKDNFLIDKNNKKILAYLDKKTTKVTIPSSIKEIDESAFFGCRNLKEVILNEGLEKIRAGAFLNTKIESITIPDSVKEIGSLAFNFSKISHISINSNNKNLEIKDNFLIDKNNKKILAYLDKKTTKVTIPSSIKEIGKGAFYGCRNLEEVILNEGLEKIGVRAFSSPRIKSINIPGSVKEIGEGALSIWDIRSISINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPSSIKEIGEDAFYGCGNLEEVILNEGLEKIGAAAFRTIKIESITIPSSLKEIGYLAFDFSKISHISINSYNKNFKIKDNFFIDKKNKKILAYLDKKTTKVTIPRSIKEIGKGAFSDCKNLEELILNEGLE", "aa_hexdigest": "592fe8a2a71659f0fd54baa8a2ae39ff", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 280804, "stop": 282600, "observations": { "mutation": [ 281094, 282528 ], "stop": [ 281094, 282528 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9V9", "query_cov": 1.0, "subject_cov": 0.5267080745341615, "identity": 0.934, "score": 630.0, "evalue": 7.68e-219, "gene_start": 1, "gene_end": 424, "reference_start": 189, "reference_end": 612, "reference_sequence": "MKGKKMKNKKNSKIWLILSSISALTAILPLATISCGDGVKQNSKENKEDLKPDESKPEPKPEDKPGTTPEVKLEPKPEDKPGTTPEVKPEDKPEPNPEPKPEPKPEPKPENPTKEEDLLNFRKNFKFELTKSNTWKITKFIGNDSKVTIPKEYQGKNITEIGNGTFSNSFINSVSITGSIKEIGERAFLGCRNLEEVILNEGLEKIGVRAFLNTKIESITIPDSVKEIGSLAFNFSKISHISINSNNKNLEIKDNFLIDKNNKKILAYLDKKTTKVTIPSSIKEIDESAFFGCRNLKEVILNEGLEKIRAGAFLNTKIESITIPDSVKEIGSLAFNFSKISHISINSNNKNLEIKDNFLIDKNNKKILAYLDKKTTKVTIPSSIKEIGKGAFYGCRNLEEVILNEGLEKIGVRAFSSPRIKSINIPGSVKEIGEGALSIWDIRSISINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPSSIKEIGEDAFYGCGNLEEVILNEGLEKIGAAAFRTIKIESITIPSSLKEIGYLAFDFSKISHISINSYNKNFKIKDNFFIDKKNKKILAYLDKKTTKVTIPRSIKEIGKGAFSDCKNLEELILNEGLEWIDALAFDFSIISHISINSNNKNFEIKDNFFIDKNNKKILAYLDKKTTKVTIPSSVKEIGESAFSDCKNLEEVSLNEGLEKIGAEAFSNTNIESIIIPDSVKEIGRRAFSSCINLEEVSLNEGLEKIGAEAFSNTNIESIIIPDSVKEIGRRAFSNCKNLKEVTYNGDASKINWNWWNIGIDTTKTKIISSNN" }, "paralog": false, "identity": 0.9949832775919732, "score": 999.0, "evalue": 0.0, "description": "Internal stop codon at 281094, 282528. Nonsense mutation around 281094, 282528.", "psc": { "uniref90_id": "UniRef90_A0A454C9V9", "product": "Leucine-rich repeat protein", "uniref50_id": "UniRef50_A0A454C9V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9V9", "UniRef:UniRef50_A0A454C9V9" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9V9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9V9" ], "product": "Leucine-rich repeat protein" } }, "id": "GLJLFKCOHC_512", "locus": "GCA013368035_00489" }, { "type": "cds", "sequence": "contig_1", "start": 282590, "stop": 282910, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTAGGATTTTCTGGTTTTGGTTCGGGTTTTGGTTCTGGGTTAGGTTCAGGTTTATCCTCTGGTTTAACTTCTGGTGTTGTTCCTGGTTTATCCTCTGGTTTAGGTTCTGGTTTACTTTCATCAGGCTTTAAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTCCATCACCACAAGAAATAGTTGCTAATGGAAGTATAGCAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATTTTCTTACCTTTCATTATTATTTATAATTTTATTTTTATATATATTAAAAAATAA", "aa": "MVGFSGFGSGFGSGLGSGLSSGLTSGVVPGLSSGLGSGLLSSGFKSSLFSFEFCFTPSPQEIVANGSIAVNAEILLNISHILLFFLFFIFLPFIIIYNFIFIYIKK", "aa_hexdigest": "4d14b4c03f79ba3901fcdac90fe51f27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11148.886299999986, "isoelectric_point": 6.516158485412597 }, "id": "GLJLFKCOHC_513", "locus": "GCA013368035_00490" }, { "type": "cds", "sequence": "contig_1", "start": 283121, "stop": 284266, "strand": "-", "frame": 1, "gene": "ftsZ", "product": "cell division protein FtsZ", "db_xrefs": [ "COG:COG0206", "COG:D", "GO:0000910", "GO:0003924", "GO:0005525", "RefSeq:WP_044730105.1", "SO:0001217", "UniParc:UPI0005CC9AE8", "UniRef:UniRef100_UPI0005CC9AE8", "UniRef:UniRef50_A0A3L9ZYL3", "UniRef:UniRef90_D1J814" ], "nt": "ATGGCAAAAGAATTAAATAATTATAACCCGGTGGCAAAAATAATTGTTATTGGAGTAGGTGGGGGCGGAAATAATAGTGTTGAAACTATGATAAATAGCCATTTAGATAGTTTTCAAATTATTGCTGCCAATACCGATAAACAAGTATTAGCAAAATTTCCTCAAGAATGCGTTTTACATTTAGGTGATGAACGTGGAATTGGCGCGGGTGCTAACCCTGAAATTGGCAAAACTGCTGCTGAATCTTCACGTGAAGAAATCAAAAGTAGATTGCAAGGAGCTGATCTAGTAATTATAACTGCAGGAATGGGCGGTGGAACCGGCACAGGTGCAGCGCCCGTGATTGCAAAAATAGCCAAGGAATGCAATGCTTTAGTAGTTGCAGTTGTAACTACTCCGTTTGACTTTGAAGGTCCAAAGAGAATGAGAATTGCAAAGCAAGGCTTACAAGAAATTAAAAAATGTGTTGATTCATATATTGTTATTTCAAACAATAAACTATTACAACAATATGGAAATATTTCTTTTTCAGATGCATTTATTTGCGCAAATAATGTCTTAAAACAAACTATTAGAACAATAGTTGATGTTATTGCCACCCCAAGCATTATTAATCTTGATTTTGCAGATTTATCAACCATTATTAAAAATAAAGGTGAAACAGTAATCGGCATTGGCCAAGCAAATGGCCAAGATCGGGCTGTAAAAGCAATCACATCTGCAATAACAAGCCCAATTTTAGAATCAAGTGTTGTTGGGGCTAGTGATGCAATAGTAAATTTCAGTGCTTCTCAAAAAGTAACTTTAAATGAAATTCAAAGTGCTTTGGGAGCTATGAGGGAAATTGTTGGTAATGAAATTAACATTATTTTTGGTATAACAACTCTAGAATCTGAAGAAAGCAACAAATTAGGTGAGCTATTTGTCTCGGTAATCGCCACGGGATTAAGAAAAGACGCTCCTAAAGACATTGATCAAATTCAAGATGAAGTAATCAATGTAATAAAAAAAGACGATTTAAATTATGTTAACGATGAAACAAAGGAATTCTTTGTATCTGAAGGAACATTTAAAACACAATCATTTTTTTCAATGGATAATGATGAAGATTCAAACGATGAACTTGCAGATATATTAAAACACTAA", "aa": "MAKELNNYNPVAKIIVIGVGGGGNNSVETMINSHLDSFQIIAANTDKQVLAKFPQECVLHLGDERGIGAGANPEIGKTAAESSREEIKSRLQGADLVIITAGMGGGTGTGAAPVIAKIAKECNALVVAVVTTPFDFEGPKRMRIAKQGLQEIKKCVDSYIVISNNKLLQQYGNISFSDAFICANNVLKQTIRTIVDVIATPSIINLDFADLSTIIKNKGETVIGIGQANGQDRAVKAITSAITSPILESSVVGASDAIVNFSASQKVTLNEIQSALGAMREIVGNEINIIFGITTLESEESNKLGELFVSVIATGLRKDAPKDIDQIQDEVINVIKKDDLNYVNDETKEFFVSEGTFKTQSFFSMDNDEDSNDELADILKH", "aa_hexdigest": "086475c7ddbec07e41228f5e2f5aeda0", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005CC9AE8", "ncbi_nrp_id": "WP_044730105.1", "uniref100_id": "UniRef100_UPI0005CC9AE8", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CC9AE8", "RefSeq:WP_044730105.1", "UniRef:UniRef100_UPI0005CC9AE8" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CC9AE8", "uniref90_id": "UniRef90_D1J814" }, "psc": { "uniref90_id": "UniRef90_D1J814", "gene": "ftsZ", "product": "cell division protein FtsZ", "uniref50_id": "UniRef50_A0A3L9ZYL3", "cog_id": "COG0206", "cog_category": "D", "go_ids": [ "GO:0000910", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3L9ZYL3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3L9ZYL3" ], "product": "Cell division protein FtsZ" }, "genes": [ "ftsZ" ], "id": "GLJLFKCOHC_514", "locus": "GCA013368035_00491" }, { "type": "cds", "sequence": "contig_1", "start": 284274, "stop": 284414, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGCAATAGATAATATTTTAGAATGTCAAATCAAACAAAATGCTTTAAATAATGAAATTAGTAGGGAATTATTTACAATTGAAGTTTATAGTAATTCCAAAAAGTATTACAATTTAATGCTAAACGATATAAATTAG", "aa": "MLAIDNILECQIKQNALNNEISRELFTIEVYSNSKKYYNLMLNDIN", "aa_hexdigest": "18d5c39f4a1dba767c77377ba2b8f4bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5438.170000000001, "isoelectric_point": 4.655875205993653 }, "id": "GLJLFKCOHC_515", "locus": "GCA013368035_00492" }, { "type": "cds", "sequence": "contig_1", "start": 284673, "stop": 285008, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTTAAACTAGTTAAAAAAACTAATATTAAGCAAGTTTATTCTTTAAAAACAATATCAAATGCTAATAGTCTAAGATTGCAAGAAATATTTAGTAGCAACGATTTAAAAATTGCCAAAATATTTTGCTTAGATAGTTTAAAGACAAATTCAGTTTTGTTTAAAAATTCAAAACAAAAAGTAGCTATTTTTGTTGATGTATTAGATGATAAAGCTAAATTTATTTTAAACATTAATAAATCAAATATAGTAAGCCAAGTATTAGATTTAGGACTAAACAAATTAAAAGCAAGAATGTTACAATGCAATTTATTAAGTACAGAAAGCAATTGA", "aa": "MEFKLVKKTNIKQVYSLKTISNANSLRLQEIFSSNDLKIAKIFCLDSLKTNSVLFKNSKQKVAIFVDVLDDKAKFILNINKSNIVSQVLDLGLNKLKARMLQCNLLSTESN", "aa_hexdigest": "4a69b394779e7889bd7591567e80179f", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12580.710499999983, "isoelectric_point": 9.76794338226318 }, "id": "GLJLFKCOHC_516", "locus": "GCA013368035_00493" }, { "type": "cds", "sequence": "contig_1", "start": 285108, "stop": 285341, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7", "UniRef:UniRef90_UPI001E400CB7" ], "nt": "ATGAAAAAAATTATTATTGCTGCATATAAATTTTTAAAAAATAGTATTGAAGTAGAAGTTATGGAAAAAACTAATACTTTATCTTTTTGTATTTATAAAAAAGCAATTAATAATATTTATTTAACTGAAAGCGATATAAAAAATTTTATAAAGCAAACAAAGAATGAAATTCAAAAATTAATATCAGCAAGCATAAATGAAGTTATTGTTATGTTGTCTGAAGATAGCAAATGA", "aa": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSK", "aa_hexdigest": "29c293c4bbf54aa26858a8c72d03686c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 285020, "stop": 285341, "observations": { "insertion": [ 285050 ], "mutation": [ 285110 ], "stop": [ 285110 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E400CB7", "query_cov": 1.0, "subject_cov": 0.7264150943396226, "identity": 1.0, "score": 147.0, "evalue": 2.03e-44, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKKIIIAAYKFLKNSIEVEVMEKTNTLSFCIYKKAINNIYLTESDIKNFIKQTKNEIQKLISASINEVIVMLSEDSKWNINSKLLTLNNDNFNISNLKILDQTSII" }, "paralog": false, "identity": 0.9905660377358491, "score": 194.0, "evalue": 1.8e-65, "description": "Internal stop codon at 285110. Frameshift due to insertion around 285050. Nonsense mutation around 285110.", "psc": { "uniref90_id": "UniRef90_UPI001E400CB7", "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E400CB7", "UniRef:UniRef50_UPI001E400CB7" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E400CB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E400CB7" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8904.433299999999, "isoelectric_point": 9.04112682342529 }, "id": "GLJLFKCOHC_517", "locus": "GCA013368035_00494" }, { "type": "cds", "sequence": "contig_1", "start": 285442, "stop": 286248, "strand": "-", "frame": 2, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "db_xrefs": [ "COG:COG0275", "COG:J", "EC:2.1.1.199", "GO:0016434", "SO:0001217", "UniRef:UniRef50_Q6KHR2", "UniRef:UniRef90_D1J816" ], "nt": "ATGGAACATATTCCAGTTCTATTAAATGAAGTAATAGAACAATTATCAATAAAACCAGACGGAATATATGTTGATTTAACATTAGGACGTGGTGGTCATAGCCAAGAAATTTTAAAAAGACTAACAACTGGAAAATTAATTTGTTTTGATAAAGATATTCAAGCTATAAACGAAAGTAGAGAAAAATTAATAAAAATAAGCCAAAACTTTATTTTAATAAAAAGTGATTTTAGAAACATTACTTGTGAACTAAAAAAACTAAATATCGATAAAGTCGATGGAATATTAGCAGATTTAGGAGTTTCTAGTCCTCAATTAGATGATCCAAATAGAGGGTTTTCTTATTCAATCAATTCTGATTTAGATATGAGAATGGATTTAGATAATACTCTAACTGCAAAAGAAATTATTAATAATGCAACTGAAAAAGAAATTATAGATATATTGCAATCTAATGCTGATGTAAAATTAAGTAATTTAATTGCGAAAGCTATTGTAAAAAATCGACCAATATCTGATTCATTTACGTTAAATCAAATAATAAAGGATTCATTGCCAGCTAAAATAGTAAGACAAAAAAATCCTTCTAAAGCAGTTTTTCAAGCTTTTAGAATAGCAGTGAATGATGAGCTAAATGCCTTAAGTGAAATGCTAATCCAACTTCCCAATTTATTAAAAAAAGACGGAAAAATAGCGATTATAACATTTCATAGTAAAGAAGACGTTATAGTTAAAAAGTTTTATCAAAATTTAAATTATTTTGACCCTAAGTTAAAAAAAATACTAATTGTTGAAACTCATAAGTGA", "aa": "MEHIPVLLNEVIEQLSIKPDGIYVDLTLGRGGHSQEILKRLTTGKLICFDKDIQAINESREKLIKISQNFILIKSDFRNITCELKKLNIDKVDGILADLGVSSPQLDDPNRGFSYSINSDLDMRMDLDNTLTAKEIINNATEKEIIDILQSNADVKLSNLIAKAIVKNRPISDSFTLNQIIKDSLPAKIVRQKNPSKAVFQAFRIAVNDELNALSEMLIQLPNLLKKDGKIAIITFHSKEDVIVKKFYQNLNYFDPKLKKILIVETHK", "aa_hexdigest": "a43bcc757546ccf2e6ade57a2ef40b8e", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "psc": { "uniref90_id": "UniRef90_D1J816", "query_cov": 1.0, "subject_cov": 0.8933333333333333, "identity": 0.996, "score": 513.0, "evalue": 6.42e-183, "valid": true, "gene": "rsmH", "product": "16S rRNA (cytosine(1402)-N(4))-methyltransferase RsmH", "ec_ids": [ "2.1.1.199" ], "uniref50_id": "UniRef50_Q6KHR2", "cog_id": "COG0275", "cog_category": "J", "go_ids": [ "GO:0016434" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KHR2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR2" ], "product": "Ribosomal RNA small subunit methyltransferase H" }, "genes": [ "rsmH" ], "id": "GLJLFKCOHC_518", "locus": "GCA013368035_00495" }, { "type": "cds", "sequence": "contig_1", "start": 286249, "stop": 286689, "strand": "-", "frame": 2, "gene": "mraZ", "product": "Transcriptional regulator MraZ", "db_xrefs": [ "COG:COG2001", "COG:J", "RefSeq:WP_044329513.1", "SO:0001217", "UniParc:UPI0005C96F93", "UniRef:UniRef100_UPI0005C96F93", "UniRef:UniRef50_Q6KHR3", "UniRef:UniRef90_D1J817" ], "nt": "ATGTTTGGTAAATATTATCGCCAAATTGATGATAAAAATAGAGTTGTTGTTCCGCCTAAATTACTACAAGAATTAGGCAAAGAATTCTATATCACAATTGGAATAGATAAAAACTTAGTATTAAGAACGGTTTCTGAGTTTCAAAAAATGAAAACTAAATTGGAAGAAGGAAACACACTTAACAAAAACGTAAGAGATTTCATTCGCTACATTTTTGGAAATACTGAAGTTGTATCTCCTGATAAAAATAACAGAATCATGATTCCCAAACATCTTTTAAATAAAGCCACTATCGACAAAGAAGTTGTTTTCCTAGGAACTGGTGATACTTGTGAATTATTTGCTAAAGAAGTTTATGATGAACAAGAAAGCTACTATGAAAATGATGATAATGTAAATGACTTGGCTCAAAAATTATTTGAAGCAGGAGTTAAGTTCTAA", "aa": "MFGKYYRQIDDKNRVVVPPKLLQELGKEFYITIGIDKNLVLRTVSEFQKMKTKLEEGNTLNKNVRDFIRYIFGNTEVVSPDKNNRIMIPKHLLNKATIDKEVVFLGTGDTCELFAKEVYDEQESYYENDDNVNDLAQKLFEAGVKF", "aa_hexdigest": "13b5d547aa9ee30fb47d3c5bb5588cd8", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0005C96F93", "ncbi_nrp_id": "WP_044329513.1", "uniref100_id": "UniRef100_UPI0005C96F93", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C96F93", "RefSeq:WP_044329513.1", "UniRef:UniRef100_UPI0005C96F93" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C96F93", "uniref90_id": "UniRef90_D1J817" }, "psc": { "uniref90_id": "UniRef90_D1J817", "gene": "MraZ", "product": "Transcriptional regulator MraZ", "uniref50_id": "UniRef50_Q6KHR3", "cog_id": "COG2001", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_Q6KHR3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KHR3" ], "product": "Transcriptional regulator MraZ" }, "genes": [ "mraZ" ], "id": "GLJLFKCOHC_519", "locus": "GCA013368035_00496" }, { "type": "tRNA", "sequence": "contig_1", "start": 286881, "stop": 286956, "strand": "+", "gene": "trnT", "product": "tRNA-Thr(cgt)", "amino_acid": "Thr", "anti_codon": "cgt", "score": 79.9, "nt": "GCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 286914, 286916 ], "id": "GLJLFKCOHC_16", "locus": "GCA013368035_00497" }, { "type": "tRNA", "sequence": "contig_1", "start": 287047, "stop": 287121, "strand": "+", "gene": "trnN", "product": "tRNA-Asn(gtt)", "amino_acid": "Asn", "anti_codon": "gtt", "score": 77.6, "nt": "GCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCA", "db_xrefs": [ "SO:0000257" ], "anti_codon_pos": [ 287079, 287081 ], "id": "GLJLFKCOHC_17", "locus": "GCA013368035_00498" }, { "type": "tRNA", "sequence": "contig_1", "start": 287126, "stop": 287201, "strand": "+", "gene": "trnE", "product": "tRNA-Glu(ttc)", "amino_acid": "Glu", "anti_codon": "ttc", "score": 66.5, "nt": "GGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCA", "db_xrefs": [ "SO:0000259" ], "anti_codon_pos": [ 287159, 287161 ], "id": "GLJLFKCOHC_18", "locus": "GCA013368035_00499" }, { "type": "tRNA", "sequence": "contig_1", "start": 287204, "stop": 287279, "strand": "+", "gene": "trnV", "product": "tRNA-Val(tac)", "amino_acid": "Val", "anti_codon": "tac", "score": 82.1, "nt": "GGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCA", "db_xrefs": [ "SO:0000273" ], "anti_codon_pos": [ 287237, 287239 ], "id": "GLJLFKCOHC_19", "locus": "GCA013368035_00500" }, { "type": "cds", "sequence": "contig_1", "start": 287404, "stop": 288408, "strand": "-", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE", "UniRef:UniRef90_UPI00280AD9DE" ], "nt": "TTGGTTATATTAATAGTTACTGTAACACTTGTATTAAAGGGAAATAATATTCATGTTTATGGGCTTTTTAATACAAATGCAGAACATGGATTTTTAATAAGTTATTTAACACTTATATATAGTTTTTTATCAACACTTCAATCAATGCTTACTATATTAGTTTATGCAAGAATTGGAATTGCATCTGCCGTTAGGGTTAATACACTTTTAAGCCTACCAAACACACATATAAAAAACCACTCAAAGGGAATTGTGATAGAAAATCCTGTTGGAAATATATCATTTGATCATGTATATTTTAGATATAATGAAAATTTGCCTAAATATCAACTAATAGATGCTTCATTTAATGCAAAAGCAGGACAAACTATTGCTTTAGTTGGAGAAACTGGAGCAGGTAAAACTACTATTATAAACTTATTGAATCGTTTTTATGATTATGAAAAAGGTTCTATAAAGATAGATGGAATAGAGCTAAGAGAAATTTCAAAAGACAGTTTAAGAGATTTTACGGCAGTTGTTTTACAAGATAGCTTTATGTTTAATGAAAGTATTTTAAATAACCTTTTAGTAGGAAATCCAAAAGCAACCAAAGAAGATGTAATAAGGGCAGCAAAATTAGCATCTGCTCATCATATTATTGAAAAATTAGAAAATGGATATGATACTATAATTGATAATAGCACTTCAATATTATCTAAAGGAGAAAAGCAACTTCTTTCAATAGCTAGAGCAATATTGGGAAACAAAAAGATCTTAATATTAGATGAAGCAACATCGAACGTGGATTCTAATACAGAAAAAATAATTCAACAAGCATTAAAAGAAAATATCATGGTTGGTAAAACATCAATAGTTATTGCCCACCGTCTAAGCACGATTAAAAATGCTGATTTAATCTTAGTTGTGGATGATGGTCGTATTATTGAACAAGGAACCCATAAGCAATTGCTAGAATTAAATAAAAAATATGCAAGACTATATTATTCACAATTTGATGATTAA", "aa": "MVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPNTHIKNHSKGIVIENPVGNISFDHVYFRYNENLPKYQLIDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKEDVIRAAKLASAHHIIEKLENGYDTIIDNSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD", "aa_hexdigest": "a005186e46c6a4e3a0215b1eeefb53cf", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 287404, "stop": 288708, "observations": { "mutation": [ 288432 ], "stop": [ 288432 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280AD9DE", "query_cov": 1.0, "subject_cov": 0.6958333333333333, "identity": 0.9790000000000001, "score": 619.0, "evalue": 5.88e-221, "gene_start": 1, "gene_end": 334, "reference_start": 147, "reference_end": 480, "reference_sequence": "MIIKNQVILFLFLVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINPWIVFTNNILVILIVTVTLVLKGNNIHVYGLFNTNAEHGFLISYLTLIYSFLSTLQSMLTILVYARIGIASAVRVNTLLSLPSTHIKNHSKGIVIENPVGNITFDHVYFRYNENSPKYQLTDASFNAKAGQTIALVGETGAGKTTIINLLNRFYDYEKGSIKIDGIELREISKDSLRDFTAVVLQDSFMFNESILNNLLVGNPKATKEDVIRAAKLASAHHIIEKLEHGYDTIIDDSTSILSKGEKQLLSIARAILGNKKILILDEATSNVDSNTEKIIQQALKENIMVGKTSIVIAHRLSTIKNADLILVVDDGRIIEQGTHKQLLELNKKYARLYYSQFDD" }, "paralog": false, "identity": 0.9838709677419355, "score": 818.0, "evalue": 4.33e-302, "description": "Internal stop codon at 288432. Nonsense mutation around 288432.", "psc": { "uniref90_id": "UniRef90_UPI00280AD9DE", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280AD9DE", "UniRef:UniRef50_UPI00280AD9DE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280AD9DE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280AD9DE" ], "product": "ABC transporter ATP-binding protein" } }, "id": "GLJLFKCOHC_520", "locus": "GCA013368035_00501" }, { "type": "cds", "sequence": "contig_1", "start": 288430, "stop": 289131, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGTTTTGTTTTCATTTTTTCATGCAATTTTTTATGCAATAGGTTCATTTTTAATTGGATATATTTTTACAATATGTTTAACAAAAGATGTATTAGATCATAAAACACCTTTTAATTTAAAATTATTTATAGGTATGGCTTCATGTTTGGTTATATCGTTTATATTGTATGGAATTTTTAGATATGTCTCAGGGGTATTCTTTGTAAAAATAACTTTTTCAGTTTGTGAGAATATAAGAAAAACCGCCATGAAAAACCTTTTATATATTCCTGTTTCGTATTTTGATAATCAAAAATCAGGTAATATTATTTCTATTTTAGTAAATGATATTAATAGTTTATCTGAAACTATATTTAAATTATTAAACGAATTAATCCAATGTGCTATAAATGTTATTTTATCTGTAATATTTTTATCAATGGTTAGCTTAGTATTATCTGCTGTTGTTTTACCTGTTCTATTCTTATTTTCATTTATAGGAATATTAATTACTAAAAAAGCTCAAAAAGCATTTATTCAAACCAGAAAACATTTTGGGGAATTAAACGCTTTTGTCGAAGAAATGCTAACTAATTCTAAAATTATTAAAACTTTCGAACAACAAGAACAAGTTAATAATCATGGTAAACAAATAGCAAGAAACATATTTAACACAGCCTTCACAGGAGATCTTAATAACCAAATAATTAACCCTTGA", "aa": "MAVLFSFFHAIFYAIGSFLIGYIFTICLTKDVLDHKTPFNLKLFIGMASCLVISFILYGIFRYVSGVFFVKITFSVCENIRKTAMKNLLYIPVSYFDNQKSGNIISILVNDINSLSETIFKLLNELIQCAINVILSVIFLSMVSLVLSAVVLPVLFLFSFIGILITKKAQKAFIQTRKHFGELNAFVEEMLTNSKIIKTFEQQEQVNNHGKQIARNIFNTAFTGDLNNQIINP", "aa_hexdigest": "511f8b17b57075d707311b7b34376e9d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26396.00490000002, "isoelectric_point": 9.158330345153807 }, "id": "GLJLFKCOHC_521", "locus": "GCA013368035_00502" }, { "type": "cds", "sequence": "contig_1", "start": 289132, "stop": 289227, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAACTAAAGAAAAGCAAAAACTTTCATTTATTACTTTGTTTAAATTAGTTTTTAAATATTCAGGTGAATCAAAAACAAAATTTTGA", "aa": "MKKRTKEKQKLSFITLFKLVFKYSGESKTKF", "aa_hexdigest": "9e033a066f30cb864e5c512b3f8fafc4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3770.5296000000008, "isoelectric_point": 10.363437843322753 }, "id": "GLJLFKCOHC_522", "locus": "GCA013368035_00503" }, { "type": "cds", "sequence": "contig_1", "start": 289242, "stop": 290210, "strand": "-", "frame": 3, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "nt": "GTGGTGCTTTATTCATTAGTTGTTCATGATGTTATTTCTAATGTAGCAACATATGATACAATTGTAAGATTTAATATTTTTATTGACTATTCATTTTATGTAACATTTGGTGTTATATTGGGGGCAGTATTTATTTCTGCATTTGTAAAAGGAAAAATTTCTGCTACTAGAGTTATAGAAGTATTAAATACTAACGAAGATAAAATTTATGTATCAAATGGTAAAAAAATAACAAAAAACTTTGATTTAAAAGTTGAAAATTTAAGCTTCAAATATTACTCTTCAAATCCAAATTATACTTTACACAATATAAGTTTTAGCCTACCGTTTGGAAAAACTCTTGGATTAGTTGGCCCTACTGGAAGTGGTAAAAGCATACTTGCAAATTTATTGGTTAATAACTATCTATATAATGAAGGATCGATTTTAATTGGGGATAATGAAATTAAAGAAGTTAATACAAAAAATTTACATGAAACGATTGGAATTGTCTACCAAGATGCCCTATTAAATACAGGAACAATAAAAGAAAATATGCTTTTTGCTAAAAATGATGCAACAGATGAAGAAATAAATCAAGCATTACGTGACGCTTGTGCATATGATTTTGTTTATAAATTTGAAGAAAATTTAGATCATGTCATTACTCAAGGGGCAACAAACCTTTCAGGTGGCCAAAAACAACGTCTTTCAATTGCAAGAACTTTATTAACTAAACCAAAAATTTTAATATTAGATAACTCAACTAGTGCTTTAGATAATATTACTGCACACCAAGTTTTACAAAATATAAAAAATAAATATTCATGTTCAACTATAATAATTAGCCAAAAATTGCCATTAATAAAAAATGCTGATGAAATTATTGTTTTATCTGATGGAAATATGTTGGATAAAGGAAATCATGAAGAATTAATGAAATCATGTTCATATTATAATGAATCATATAATAACCAATTAAGCCAATAG", "aa": "MVLYSLVVHDVISNVATYDTIVRFNIFIDYSFYVTFGVILGAVFISAFVKGKISATRVIEVLNTNEDKIYVSNGKKITKNFDLKVENLSFKYYSSNPNYTLHNISFSLPFGKTLGLVGPTGSGKSILANLLVNNYLYNEGSILIGDNEIKEVNTKNLHETIGIVYQDALLNTGTIKENMLFAKNDATDEEINQALRDACAYDFVYKFEENLDHVITQGATNLSGGQKQRLSIARTLLTKPKILILDNSTSALDNITAHQVLQNIKNKYSCSTIIISQKLPLIKNADEIIVLSDGNMLDKGNHEELMKSCSYYNESYNNQLSQ", "aa_hexdigest": "42a94e5b35c1b29f24fb586876b7b8e3", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DFCB5D", "query_cov": 0.9440993788819876, "subject_cov": 0.9589905362776026, "identity": 0.52, "score": 315.0, "evalue": 1.35e-103, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI0021DFCB5D" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFCB5D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFCB5D" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_523", "locus": "GCA013368035_00504" }, { "type": "cds", "sequence": "contig_1", "start": 290289, "stop": 290546, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAATTGGTGGATGTATTTTAGCCGTCATGAATAACGTAAAAATGGCAATAGGATTAATTTTGTTAGTTCCGATAATTTTAACAATAGTTTCTATTATGGCCAAAAAAGCAAGTCCTTCAATTAAAAAAGCTCAATTAGCCGTTGAAGAAATAACTAAGCAAGTTGATGAAAATATATTAGGATCTCGTTTAATAAAAATATATGATTTATATAAAAATAGAGAAGAAAAGTTTGCAAAAATAAATAAAACATGA", "aa": "MIIGGCILAVMNNVKMAIGLILLVPIILTIVSIMAKKASPSIKKAQLAVEEITKQVDENILGSRLIKIYDLYKNREEKFAKINKT", "aa_hexdigest": "f4b0a80b858dff6d1eaa5909d85d28fe", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9426.415499999992, "isoelectric_point": 9.673819541931152 }, "id": "GLJLFKCOHC_524", "locus": "GCA013368035_00505" }, { "type": "cds", "sequence": "contig_1", "start": 290592, "stop": 290984, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTGTAGGAAGCTTTATTTCTTTAATTTCGGTTATATTTGAATTGATTACTCCTACATTGATTTCTCAATTCATTAGATTGCTTTTTATTGAAAATAAAAATAGTCAAGTAGATTTAAGTTTTTTTAATGGATTAATAAACTTTACATTTAATAATGCTATTAATGCTCGTAATTATATGATTTATTCTATAATAGTTTTAACTATAATTTCAATGTCTTTAACATTTGGCTGCACTAGCTTAATTGTATATGCAGCAGAAAGAAGTAGTAGATATATTAGAGAAAACTTTATTAGAAAATTAAATACATTAAGTTTAAAAAATATATCTGATTTGAAACCTGAATCATTAATTACTAGGGTGAGTGATGATATTGCGGTTTTCTGA", "aa": "MFIVGSFISLISVIFELITPTLISQFIRLLFIENKNSQVDLSFFNGLINFTFNNAINARNYMIYSIIVLTIISMSLTFGCTSLIVYAAERSSRYIRENFIRKLNTLSLKNISDLKPESLITRVSDDIAVF", "aa_hexdigest": "62971badabec682713fe13ec023314ec", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14847.25269999998, "isoelectric_point": 8.88685398101807 }, "id": "GLJLFKCOHC_525", "locus": "GCA013368035_00506" }, { "type": "cds", "sequence": "contig_1", "start": 291212, "stop": 292027, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAATCTTACTGTTTCATTTAAAGATTATGAAAATAGTATTAAGAAATCAAAATTTGTTGAAATTGATTTAAGCAAATTGGGTGTTGATGAATATAATAAGATTAGAAAACAAAATAATGAAACTGAAATAGAAGACCAACAAGCCCCAACATCAACTATTGAAGACCAAGATGTAAAAGAATTAAATAAAGATGCATTCTTAAAATATTTCAAAGAAAACAAAGATAAATTATCTAACGATGAAGTTAAAAATAATTCAACATTTATGTTTATAGAAAAATTGAAAAATATTAGTCTTTTAAGCAAAAACATTGAAAAAGAAATGATTGAAAAAGGTGATAATTACATTTCGGTTCAAAATTTTGATTATGATAAAACAACATTAAAATTAAAACATTCAATGTTTGCATTTGAATATACAAAACATATGGTTGATGTACAAAATGTTTATATCTTTTCATTTCCTAAAATAGAAAATAACAAAGTAAAATCAATTAGAGTTTCTATAGTTTCTCTTCAAAATTTTCAAAATAATGATTTTAGCAACATGTCATCTAAACGTGTTAATGTTAATGAAAAGATAAATGAAGATAAAGACTTTATGCAAAAAATAGTTTTAAAAGAACATTTTAGAAAATTAGTAACCGTAAATTACATTGGCGATAAAAAAACAGTTGATGAAGCAATTAAAGATGATATTTCTAAATTCGAATACAAATTGCCAGAAGGATTAACATTTGAAAAAATCTCAGTTAAAAAATATACAAGCAAAGGTAAAGAATATATAAAATATACAATCACTGCTAAATGA", "aa": "MINLTVSFKDYENSIKKSKFVEIDLSKLGVDEYNKIRKQNNETEIEDQQAPTSTIEDQDVKELNKDAFLKYFKENKDKLSNDEVKNNSTFMFIEKLKNISLLSKNIEKEMIEKGDNYISVQNFDYDKTTLKLKHSMFAFEYTKHMVDVQNVYIFSFPKIENNKVKSIRVSIVSLQNFQNNDFSNMSSKRVNVNEKINEDKDFMQKIVLKEHFRKLVTVNYIGDKKTVDEAIKDDISKFEYKLPEGLTFEKISVKKYTSKGKEYIKYTITAK", "aa_hexdigest": "f00d8e30475e6eab9bff39aad472bdcf", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 31951.078900000008, "isoelectric_point": 8.756112098693851 }, "id": "GLJLFKCOHC_526", "locus": "GCA013368035_00507" }, { "type": "cds", "sequence": "contig_1", "start": 292094, "stop": 293011, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821", "UniRef:UniRef90_D1J821" ], "nt": "TTGGTAAATTCTGAAAAACCTCTTAGAAGTGGAGACAAGTTAAACTCTAAATTGTTATTAACAAAAGATGAAAAAGGAAACTTTATTTTGAAATATAAGGTTGCAATTTTTAAACAAAATGGTAAACATATTATTTCAAACAAGGTTTATGAAAGTAATTTGGGAAACTTAGATTCTGGATTAGATCAAGTTTCAGAAGAAGATTTTAAAGAAGTTTCAAAAAATCTAGTCTTTGACTATCCTAACAAACAAGAAACATACATAAAAGACGCAGACACATCAAAAGTATTTTTAAAAACTATTAAAGAAAACTTTTCAATTGTTAAAGATTCGTTAAAAATTACTAAAAATACAGGAGAAGATGGAACATATGACTTGTCAATTCTTTACTATTTATCATACAAAAATAAACAAATTTCAAGTCAAAAATACATTTATGAAATAAAAGGTTTTAAACTAACTCCAGAATTGCAAGAAAAAATAAATAAGGCCAAAAAAGAACTAAAAGATCAAATTGAAAAAACAGATGTTTTAGTTGCAAATGCCAAGTCATATCAAGATATTTTAAAAAATAAACAAATAAACAATTTTGATAAAAAACCAAATTTTGCAGTTCAAGGCTACGACCAAGCTTCATATTCAGTAGTATTAAGCAATTTAAAAGTTGAAGATAAAAAAATAACATTGACTTTAACTTTGTCAAGTCTTTTAAATAAAGAAATAAGTGAATCTAAAACTATTGAAGTTAAATTAGATAAATTTGCAAACGGTTTAGATAATATTGCATTGCTATCACCAGAAAAACAAGCTGAACTTTTAAACAAAGCTTTAGAAACGTCAATTTATCCTTTTTATAGTAAAGACAAATATTACATTTTAAAGAAATCTATTGACAAATTAGAAAATAAATCATACTGA", "aa": "MVNSEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKINKAKKELKDQIEKTDVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVVLSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSPEKQAELLNKALETSIYPFYSKDKYYILKKSIDKLENKSY", "aa_hexdigest": "36d031816034565c09a39e4557f54075", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 291791, "stop": 293311, "observations": { "mutation": [ 292096, 293041, 293185 ], "stop": [ 292096, 293041, 293185 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J821", "query_cov": 1.0, "subject_cov": 0.5222602739726028, "identity": 0.97, "score": 552.0, "evalue": 1.96e-193, "gene_start": 1, "gene_end": 305, "reference_start": 142, "reference_end": 446, "reference_sequence": "MKKHTKILLALGSVATSVFAIPLVAAGCIKTKKPEVKPEEPKKPEGEKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKITWNAENLEFIKKVLNNLDKYKILFERNNKNIMAFEGAYDFKNQEFAIAKWNSKSGVSVQLVNPEKPLRSGDKLNSKLLLTKDEKGNFILKYKVAIFKQNGKHIISNKVYESNLGNLDSGLDQVSEEDFKEVSKNLVFDYPNKQETYIKDADTSKVFLKTIKENFSIVKDSLKITKNTGEDGTYDLSILYYLSYKNKQISSQKYIYEIKGFKLTPELQEKIDKARKELKDQIEKTNVLVANAKSYQDILKNKQINNFDKKPNFAVQGYDQASYSVALSNLKVEDKKITLTLTLSSLLNKEISESKTIEVKLDKFANGLDNIALLSFEKQDELLNKALETSIYPFYSKDKYYISKKSIDKLENKSYWINNKNSEINYTLGELKFEGDKCLINLTVSFKDYENSTKKSKFVEIDLSKLGVDEYNKIRKQNNETEIDDQQAPTSTIEDQDVKELNKDAFLKYFKENKDKLSNDEVKNISTFMFVEKLKNISLLSKDIEKRNDWKRW" }, "paralog": false, "identity": 0.9526627218934911, "score": 934.0, "evalue": 0.0, "description": "Internal stop codon at 292096, 293041, 293185. Nonsense mutation around 292096, 293041, 293185.", "psc": { "uniref90_id": "UniRef90_D1J821", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J821", "UniRef:UniRef50_D1J821" ] }, "pscc": { "uniref50_id": "UniRef50_D1J821", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J821" ], "product": "Variable surface lipoprotein" } }, "id": "GLJLFKCOHC_527", "locus": "GCA013368035_00508" }, { "type": "cds", "sequence": "contig_1", "start": 293183, "stop": 293431, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACATACAAAAATTTTATTAGCCTTAGGTAGTGTTGCAACCTCGGTTTTTGCAATACCATTAGTGGCAGCAGGTTGCGTAAAAACAAAAAAACCTGAAGTTAAACCAGAAGAACCTAAAAAACCAGAAGATAAACCAGGAACAACACCTGAAGTTAAACCAGAAGATCCAAGTCATGAAGAAGAAACAAATAAAAATGATGCTGATAAATATGGTGAAGAATCAATTTCAAAAATAACATGA", "aa": "MKKHTKILLALGSVATSVFAIPLVAAGCVKTKKPEVKPEEPKKPEDKPGTTPEVKPEDPSHEEETNKNDADKYGEESISKIT", "aa_hexdigest": "6de2363603e45c064f2e27b63c9dc470", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8912.070799999996, "isoelectric_point": 6.113454246520996 }, "id": "GLJLFKCOHC_528", "locus": "GCA013368035_00509" }, { "type": "tRNA", "sequence": "contig_1", "start": 293711, "stop": 293786, "strand": "-", "gene": "trnK", "product": "tRNA-Lys(ctt)", "amino_acid": "Lys", "anti_codon": "ctt", "score": 81.9, "nt": "GCATCCTTAGCTCAGTTGGCAGAGCAAGGGACTCTTAATCCCTGGGTCGTGGGTTCGAGCCCCACAGGATGTACCA", "db_xrefs": [ "SO:0000265" ], "anti_codon_pos": [ 293779, 293781 ], "id": "GLJLFKCOHC_30", "locus": "GCA013368035_00510" }, { "type": "tRNA", "sequence": "contig_1", "start": 293887, "stop": 293971, "strand": "-", "gene": "trnL", "product": "tRNA-Leu(tag)", "amino_acid": "Leu", "anti_codon": "tag", "score": 60.8, "nt": "GCGCGAGTGGTGAAATTGGCAGACACGTTAGTTTTAGGCACTAATGCTTTGCGGCGTGAGGGTTCAAGTCCCTCCTCGCGTACCA", "db_xrefs": [ "SO:0000264" ], "anti_codon_pos": [ 293941, 293943 ], "id": "GLJLFKCOHC_29", "locus": "GCA013368035_00511" }, { "type": "tRNA", "sequence": "contig_1", "start": 293975, "stop": 294050, "strand": "-", "gene": "trnT", "product": "tRNA-Thr(tgt)", "amino_acid": "Thr", "anti_codon": "tgt", "score": 88.2, "nt": "GCCGTTTTAGCTCAGTTGGCAGAGCAACTGACTTGTAATCAGTAGGTCGTAGGTTCAAGTCCTATAAACGGCACCA", "db_xrefs": [ "SO:0000270" ], "anti_codon_pos": [ 294015, 294017 ], "id": "GLJLFKCOHC_28", "locus": "GCA013368035_00512" }, { "type": "cds", "sequence": "contig_1", "start": 294259, "stop": 295047, "strand": "+", "frame": 1, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "db_xrefs": [ "COG:COG0130", "COG:J", "EC:5.4.99.25", "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982", "KEGG:K03177", "SO:0001217", "UniRef:UniRef50_A0A501X9B0", "UniRef:UniRef90_D1J822" ], "nt": "ATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAATTGGACATTGTGGAACATTAGATCCTGAGGCTGAAGGTCTTTTAATCGTAGCGACAGATGAAGATACAAAGGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCAAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTGCCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATTGAATTTAATGAAGTTAACCAATCAATCATCATTAAGGCCAATGTTTCAAAAGGCACATATATTAGATCAATGATTCATGATTTAGCTAATATATGCAACACAGATGCAGTGGTGGTCGTTTTAAAGCGAATTAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTCAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATAACATAGTAGGATGA", "aa": "MFYKLYKKRGQSSFFAIKKFAKNNNINKIGHCGTLDPEAEGLLIVATDEDTKALSLIENQNKEYYVEAKFHYSSDSFDLGTYVTKIQNFQIITKSQIIQALEILKKQTKQVPPIYSAKKINGTRAYKLARDNKEFSLKANDIKINNLELIEFNEVNQSIIIKANVSKGTYIRSMIHDLANICNTDAVVVVLKRIKIGNICLNGQEEFEKITNLNELFNVKLFKLTKNDIQILANQNSNSCYFESLKNFCGNIIFIYLNNIVG", "aa_hexdigest": "3647f799268530a6bf4bbbad9a484274", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J822", "query_cov": 1.0, "subject_cov": 0.9128919860627178, "identity": 0.992, "score": 504.0, "evalue": 4.97e-180, "valid": true, "gene": "truB", "product": "tRNA pseudouridine(55) synthase TruB", "ec_ids": [ "5.4.99.25" ], "uniref50_id": "UniRef50_A0A501X9B0", "cog_id": "COG0130", "cog_category": "J", "kegg_orthology_id": "K03177", "go_ids": [ "GO:0001522", "GO:0003723", "GO:0009451", "GO:0009982" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501X9B0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X9B0" ], "product": "tRNA pseudouridine synthase B" }, "genes": [ "truB" ], "id": "GLJLFKCOHC_529", "locus": "GCA013368035_00513" }, { "type": "cds", "sequence": "contig_1", "start": 295131, "stop": 295502, "strand": "+", "frame": 3, "gene": "cof", "product": "YcsE-related riboflavin metabolism phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_D1J823", "UniRef:UniRef90_D1J823" ], "nt": "ATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTGTTGCCATTCGGAGTTACTCAATTAACACCTAGAATGCAAGAAATGTTTGAAAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATTGATAACCCTTATGTTGATTATTTTGTAGGTGCAAATGGAACTTTTATTCTTGATCTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTGTTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGA", "aa": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFVGANGTFILDLRTKEYIFEKTINYKDFVEFRKYASEHKLSFSFVGNKWGYYNELFNIDH", "aa_hexdigest": "6a08c2be2ab794391f9162b6291f8e4a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 295131, "stop": 295805, "observations": { "mutation": [ 295500, 295653, 295668 ], "stop": [ 295500, 295653, 295668 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J823", "query_cov": 1.0, "subject_cov": 0.4606741573033708, "identity": 0.992, "score": 257.0, "evalue": 6.95e-85, "gene_start": 1, "gene_end": 123, "reference_start": 1, "reference_end": 123, "reference_sequence": "MTKYKMIVFDIDGTLLPFGVTQLTPRMQEMFEKLKEQGYVNVLCSGRDIFTVGAQIDNPYVDYFVGANGTFILDLRTKEYIFEKTINYKDFIEFRKYASEHKLSFSFVGNKWGYYNELFNIDHWFYRPYKDKFIPEKEFESQHDKNYLITISTPTPSIVAADLEKFFKEKNLDLWILAQWNGGIFISAKGITKAVGLKILGDLLNISLDEMVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAAKEICLPVTEDGPYWKLRDKGFFD" }, "paralog": false, "identity": 0.9821428571428571, "score": 444.0, "evalue": 6e-161, "description": "Internal stop codon at 295500, 295653, 295668. Nonsense mutation around 295500, 295653, 295668.", "psc": { "uniref90_id": "UniRef90_D1J823", "gene": "Cof", "product": "YcsE-related riboflavin metabolism phosphatase", "uniref50_id": "UniRef50_D1J823", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J823", "UniRef:UniRef50_D1J823", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J823", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J823" ], "product": "Cof-type HAD-IIB family hydrolase" } }, "id": "GLJLFKCOHC_530", "locus": "GCA013368035_00514" }, { "type": "cds", "sequence": "contig_1", "start": 295761, "stop": 295904, "strand": "+", "frame": 3, "gene": null, "product": "Hydrolase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "nt": "ATGGTGGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTAGGATTAGGTATTGCTATGGGCAATGGAGAAGATGTTACTAAAGATGCTGCAAATGAAATTTGCTTGCCAGTTACTGAAGATGGTCCATATTGA", "aa": "MVAFGDSENDIEMLEKVGLGIAMGNGEDVTKDAANEICLPVTEDGPY", "aa_hexdigest": "574b0db1bae9dc23f0128d424f25fbcc", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A2W0C783", "query_cov": 0.9361702127659575, "subject_cov": 0.8, "identity": 0.568, "score": 53.5, "evalue": 1.64e-08, "valid": false, "product": "Hydrolase", "uniref50_id": "UniRef50_A0A2W0C783" }, "pscc": { "uniref50_id": "UniRef50_A0A2W0C783", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2W0C783" ], "product": "Hydrolase" }, "genes": [], "id": "GLJLFKCOHC_531", "locus": "GCA013368035_00515" }, { "type": "cds", "sequence": "contig_1", "start": 295949, "stop": 296728, "strand": "+", "frame": 2, "gene": "spoU", "product": "tRNA/rRNA methyltransferase", "db_xrefs": [ "COG:COG0566", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9", "UniRef:UniRef90_D1J824" ], "nt": "ATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTCTAAAATATAAAAAATTACTTACTCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGTTATCATTTAGTAAAAGAAGCAATCAAGGCAAATATAGTTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAGACACTATCAACAACACAAACGCCTCAAAATATCGTTGCAATTTGTAAAATACAAAATTTAAATATTAAAGCAAATAAAATAATAGCCCTTAATAAATTACAAGATCCAGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGATTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATGACATTGATTTTAGAAATAAAAAAATTATTGTTGTTTTGGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTATATTCCTATTGAATTTGAAAGCCTCAATGTTGCTTGCTGCGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAA", "aa": "MVDFYLFYFKILILGKKHMAITSTQDPKILKYKKLLTPKGRQEQQLFIVEGYHLVKEAIKANIVIDILEFNDILTYKNSTQVTRAIIKTLSTTQTPQNIVAICKIQNLNIKANKIIALNKLQDPGNIGTIFRLAKSFGFDSVIVENIDIYNEKIIRSSQGAMFSLNIIKTNDLSKELSAFKKDDFLVYSTTLNKNSIKLNDIDFRNKKIIVVLGNEGNGIDKKIIDLSDFNVYIPIEFESLNVACCGAIIMNKIYNGDK", "aa_hexdigest": "40eeaa277f959c8ba1fdce4fb8192737", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J824", "query_cov": 0.9305019305019305, "subject_cov": 1.0, "identity": 0.971, "score": 457.0, "evalue": 6.53e-162, "valid": true, "gene": "SpoU", "product": "tRNA/rRNA methyltransferase", "uniref50_id": "UniRef50_A0A3B0PPX9", "cog_id": "COG0566", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PPX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PPX9" ], "product": "tRNA/rRNA methyltransferase" }, "genes": [ "spoU" ], "id": "GLJLFKCOHC_532", "locus": "GCA013368035_00516" }, { "type": "cds", "sequence": "contig_1", "start": 296887, "stop": 297360, "strand": "+", "frame": 1, "gene": "ribF", "product": "FAD synthase", "db_xrefs": [ "COG:COG0196", "COG:H", "EC:2.7.1.26", "EC:2.7.7.2", "KEGG:K11753", "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6", "UniRef:UniRef90_D1J825" ], "nt": "ATGATGTTTAAAAATCCAGTTAAAAATTCTCAGGTATTAACAAAAAAAGCAATGCAACCAAAAACTAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGTGATTTTATTCAAGGATTAAAAATAAACAACGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGATTTGGGTTCAATAGAGAGGGTAACATTGATAAATTAAAAAAATATTTCAATGTATATGTTGCCAATGAATTAAAGATAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAATGGCTTGAACAAATTACTAATAGACAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTCAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGA", "aa": "MMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKINKQKISSTIINEFIEEGNINGLNKLLIDKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVV", "aa_hexdigest": "a9c545f4ee8a8179146e859b35c404ff", "start_type": "ATG", "rbs_motif": null, "genes": [ "ribF" ], "truncated": "both", "pseudogene": { "start": 296728, "stop": 297600, "observations": { "mutation": [ 296743, 297358 ], "stop": [ 296743, 297358 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J825", "query_cov": 1.0, "subject_cov": 0.5358361774744027, "identity": 0.987, "score": 307.0, "evalue": 1.14e-103, "gene_start": 1, "gene_end": 157, "reference_start": 54, "reference_end": 210, "reference_sequence": "MQVFDWDFKAKLNINEPLTICLGSFETLHLGHNELFKIAKDLKQSYPDQKLAIMMFKNPVKNSQVLTKKAMQPKTRLYTLAMLGFDYVFLVDVNQTILNCSYSDFIQGLKINNVKNVVCGQDFRFGFNREGNIDKLKKYFNVYVANELKVNKQKISSTIINEFIEEGNINGLNKLLIGKYSIIVNSQKFNFKYPEHLNQLKAGIYIANVVWNNIEYHGLIFINKNQFDSNNNLQENNIIYLFDIDYFISKYQELYIEFLQCIRHINNNSENQISTNDIEIGKKYFISKEKMNS" }, "paralog": false, "identity": 0.9655172413793104, "score": 564.0, "evalue": 1.26e-207, "description": "Internal stop codon at 296743, 297358. Nonsense mutation around 296743, 297358.", "psc": { "uniref90_id": "UniRef90_D1J825", "gene": "RibF", "product": "FAD synthase", "ec_ids": [ "2.7.1.26", "2.7.7.2" ], "uniref50_id": "UniRef50_A0A448ZYJ6", "cog_id": "COG0196", "cog_category": "H", "kegg_orthology_id": "K11753", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J825", "EC:2.7.1.26", "EC:2.7.7.2", "UniRef:UniRef50_A0A448ZYJ6", "COG:COG0196", "COG:H", "KEGG:K11753" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYJ6" ], "product": "FAD synthase" } }, "id": "GLJLFKCOHC_533", "locus": "GCA013368035_00517" }, { "type": "cds", "sequence": "contig_1", "start": 297664, "stop": 298176, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAATGGCTATGAAATTGTTAAACAAATGTTTAATTATGGCCATACTTATAGAGAAAAAGAAATCAATAAAATGATAAAAGATATATTCGTGAACCTACCTTTAAATAAAGACGAATATACTTATAAATTTCTTTGTGAAAATTCTATAAATTATTTTGTTAATAATAAAATGTTAGAAAAATTAATAAATCCTGATGGCGAAATCTTTTATCATGACGTTTCAAAGCCAAATACTTTTAGAATGCCAAAGGATTGTGACAATAGAAAAATATCAGAAATAAGTTCGTTGGAAATGTTTGATTTAATAAAACAAGTTTTAAACAATTCAAAAAGTTCTATTTTAAAAAACAATTTAACTAATACTATTTTAAAAGCATTACTAATAGCAAGAACAGAAAATAATATTTCAAAATTAAATGATCTATATGGATATTTAAATAATTTAGGGTTAATTAAAGTTATTTCAAACGGCGATATTGAATATATTGACTTGAACAACAAAGATTAA", "aa": "MLNGYEIVKQMFNYGHTYREKEINKMIKDIFVNLPLNKDEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKALLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD", "aa_hexdigest": "7bd133abced4fc92d2876787d4663aaf", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19919.682900000018, "isoelectric_point": 6.740047264099122 }, "id": "GLJLFKCOHC_534", "locus": "GCA013368035_00518" }, { "type": "cds", "sequence": "contig_1", "start": 298429, "stop": 299301, "strand": "-", "frame": 2, "gene": null, "product": "AAA domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6", "UniRef:UniRef90_UPI001F43A718" ], "nt": "TTGCCTTCAAATAAGTTCGTTTATGATAATAAATTAATTACTTTTCCAACATATCAAAAAACCGCCAAGAAAGACGGGGAATTGCTTCCTTTTGAAGGCGTTTCATTTATTAAGGTTAATGGAATTTATGAAGATCAAACAAACGAAATTGAAGCAAATAGAATCATTGAATTAATAAAGCAATTAAAAGATTATTATGGCAATACAAAAACATTTGGGGTTGTAACGTTTAATTTAAAACAAGCTAACTTAATTGATCACAAATTATTCTTATTTATGAATGAAAATCCTGAATATATGGATTTAGACACAGAAAATAAAATTTTTGTTAAGAACATTGAAAGTGTTCAAGGTGATGAAAGAGACATCATTATTTTAGGAATTACAAATAGTTGGGATAAAAACGGAAGATTGTCAAGATTCTTTGGACCATTAAATAATGTTGGCGGTGAAAAGAGACTAAACGTCGCAATAAGTAGAGCCAAGGAAGCAACTATTGTTGTATCTTCAATATCATATTCTGATTTAAAAGTAGACGATTTAAAAAATGATGGACTAAGATTTTTAAAGCAATATTTACAATTTGCAGAATTTGGATACGACAGCAAATCAGATGATATATTACAAGATGACACTAAAACATTTGATAGTGAGTTTGAAATCCAAGTTTATGATGAACTTAAGAAGCTTGGATTTAAAGTAAATACTCAAGTGGGATGCTCAAAATTCAAAATAGACTTAGCAATTGTTGATGATAAAAATCCATCAAAATATATTTTAGGTATTGAATGCGATGGCGCCACATATCATTCATCAAAAAGCGCTCGTGATAGAGATGCGATTAGACAAATGCTACTTGAGTCAAGAGGTTGA", "aa": "MPSNKFVYDNKLITFPTYQKTAKKDGELLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNDGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGFKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRG", "aa_hexdigest": "ce68d9d009e6acf4382a988855b69baf", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 298126, "stop": 299274, "observations": { "mutation": [ 298245, 298401, 298413, 298431 ], "stop": [ 298245, 298401, 298413, 298431 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F43A718", "query_cov": 0.9689655172413794, "subject_cov": 0.5072202166064982, "identity": 0.993, "score": 555.0, "evalue": 3.34e-195, "gene_start": 10, "gene_end": 290, "reference_start": 19, "reference_end": 299, "reference_sequence": "MIEKSLYDFATPDFQTLPNKLITFPTYQKTAKKDGQLLPFEGVSFIKVNGIYEDQTNEIEANRIIELIKQLKDYYGNTKTFGVVTFNLKQANLIDHKLFLFMNENPEYMDLDTENKIFVKNIESVQGDERDIIILGITNSWDKNGRLSRFFGPLNNVGGEKRLNVAISRAKEATIVVSSISYSDLKVDDLKNEGLRFLKQYLQFAEFGYDSKSDDILQDDTKTFDSEFEIQVYDELKKLGFKVNTQVGCSKFKIDLAIVDDKNPSKYILGIECDGATYHSSKSARDRDAIRQMLLESRGWIIHRIWSLNWFKNKRRELEKIVDKVNQIKQKKFVVKQNEYKTIENSQFEVIEKRDNFNFEKWLPFNYSYRKTLNFIEKSNKRNPLLNGYEIVKQMFNYGHTYREKEINKMIKDIFMNLPLNKNEYTYKFLCENSINYFVNNKMLEKLINPDGEIFYHDVSKPNTFRMPKDCDNRKISEISSLEMFDLIKQVLNNSKSSILKNNLTNTILKTLLIARTENNISKLNDLYGYLNNLGLIKVISNGDIEYIDLNNKD" }, "paralog": false, "identity": 0.9764397905759162, "score": 742.0, "evalue": 2.31e-271, "description": "Internal stop codon at 298245, 298401, 298413, 298431. Nonsense mutation around 298245, 298401, 298413, 298431.", "psc": { "uniref90_id": "UniRef90_UPI001F43A718", "product": "AAA domain-containing protein", "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F43A718", "UniRef:UniRef50_UPI0002FAB5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0002FAB5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0002FAB5C6" ], "product": "AAA domain-containing protein" } }, "id": "GLJLFKCOHC_535", "locus": "GCA013368035_00519" }, { "type": "cds", "sequence": "contig_1", "start": 299332, "stop": 299769, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12" ], "nt": "TTGCCTACGTTAAATTCAACAATATACAATGATGAATTAGAAATTTTAAAGAAGGAATATTCAAAAGTTAGAAATAAAAAACCGATTAGAAAACTATTTAAAGAAATTCCAAATTTAATTCAAAATATTAAGCCTTGTTTTATGATGTCTCCTTCTTCGCTAAGTACATATTTAGCGGATAGTAGCATTACATTTGATAGTGTAATTTTTGATGAGGCTTCTCAATTAACGACAGAAAATGCCTTGATATCAATGATTAAGGCTAAACAAATTATTATTGCCGGTGATATAAATCAATTGCCACCAACTAATTTCTTTAAAACTATGTCTAGCGAGCAAGAACCAAACGAAGACGAAGAAATAGTCAACGAAGAAAATGATTATGATTCACTTCTTGAAAGAATGCAAAGCTCATTTAGACAAGTTACATTAAAATGA", "aa": "MPTLNSTIYNDELEILKKEYSKVRNKKPIRKLFKEIPNLIQNIKPCFMMSPSSLSTYLADSSITFDSVIFDEASQLTTENALISMIKAKQIIIAGDINQLPPTNFFKTMSSEQEPNEDEEIVNEENDYDSLLERMQSSFRQVTLK", "aa_hexdigest": "ec16b0df06247633108957fce7a498fc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 52, "aa_cov": 0.35172413793103446, "hmm_cov": 0.9807692307692307, "evalue": 8.481233726499032e-12, "score": 40.750946044921875, "start": 54, "stop": 104 } ], "seq_stats": { "molecular_weight": 16691.853299999988, "isoelectric_point": 4.7559118270874015 }, "id": "GLJLFKCOHC_536", "locus": "GCA013368035_00520" }, { "type": "cds", "sequence": "contig_1", "start": 299872, "stop": 300570, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGAAATCTCGAAATAATATTTTTAAGCGTATTTTTTCAAAGAGATATAGAACAATAAAAAAAGAATTAAAAATTAATTTTATATCTTCTAATTTAAATTACAAAAACTTGTTAAAAGATTTAGTGGCATTAAATAACATTCAACTTGCTAAAAATGCAATTACGCTTTTAACAAATAAAATTCCTTTTTCATTTAAAGAAAACTATAATGAAATGGATCTTAATAGTTTAAGGCAACAACTAAATATATTTTCTAAATACGAAATTATTAATAATCAATTAAGTGAATTAAAATTTGACCAAGATTTAAAAATTAGCTTTTTTAACTTTTTATTAAGCCATATTCAATTAACAAATAATTACATAAATTCATATAAAAATTTAATGACTTTTGAAGATAAATTCAATGACTACTTTAATAATAAATATGTAGATTATTCAAATTCAAATTATAAAGTTAATGATTTTATAAAAAAACTAAATTTTTTAATTGATTCTGATGAATATCCCGAAGCATATTGTAGTTATATAAAATCAAGACAAGAATTAAAAGACAATCCATTTACTAATGATTTTGTGGATGCTATTGAATTGCTAGATATTGATTGTTTAAAACAAAATAGTATTGAAAATATTTTTAATAAAAGATTTTATCAACTTTTAATTGATTACTATTTATCAATTGATTTAGCAGATTGA", "aa": "MKSRNNIFKRIFSKRYRTIKKELKINFISSNLNYKNLLKDLVALNNIQLAKNAITLLTNKIPFSFKENYNEMDLNSLRQQLNIFSKYEIINNQLSELKFDQDLKISFFNFLLSHIQLTNNYINSYKNLMTFEDKFNDYFNNKYVDYSNSNYKVNDFIKKLNFLIDSDEYPEAYCSYIKSRQELKDNPFTNDFVDAIELLDIDCLKQNSIENIFNKRFYQLLIDYYLSIDLAD", "aa_hexdigest": "82b4fd921b855cedcda67ba72e7b562a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27914.459700000036, "isoelectric_point": 8.275177955627445 }, "id": "GLJLFKCOHC_537", "locus": "GCA013368035_00521" }, { "type": "cds", "sequence": "contig_1", "start": 300631, "stop": 300927, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATGTAAAGCTTCAAGATTTAGAAAACATTAAAGAGATTTCTAGCCATTTAATAAATGAAATTCAAAGCCTAATCAATTTCTTAAATCTTGAAATTCCAAACAATTATCAACCATATATTAAAAATAGCAATTATTCATTATTTAATTCATTTTTTAAAATTTTTTCAAATATACAAGATATAGATATAAATCCAAAATATTTAGAAATTTTTTCATATTATGAAACAAATGCAACACAAACCCTAAATGAAATACTATTACAAAAAGAAAAAATTCAACCTGATATTGACTGA", "aa": "MDVKLQDLENIKEISSHLINEIQSLINFLNLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYYETNATQTLNEILLQKEKIQPDID", "aa_hexdigest": "76fb1fafaf05bf0cccb53a5fded6f656", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11650.010699999988, "isoelectric_point": 4.479219627380371 }, "id": "GLJLFKCOHC_538", "locus": "GCA013368035_00522" }, { "type": "cds", "sequence": "contig_1", "start": 300943, "stop": 301698, "strand": "-", "frame": 2, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65", "UniRef:UniRef90_UPI0024114E65" ], "nt": "ATGGACATTTATTTAGATGTTGATAAAAACAAAGATAATATCATTAATTCAGAAATTATGAATGCAATGACAGGGATTAACAATGTTTTTAGTTCTGAATATTTGTATTCTGAAGAAGAAGTTGATAATATAGTTTCTCCTAAAGGCTATTATCACTGCCTAATGACTAATTCTTCGCAAGAAGCAGCTATTCAATCAGCAATTAAAGGAAATAGTTTTATTATTCAAGGTCCTCCAGGAACTGGAAAATCTCAAACTATAGCAAATATAATAAGTGAATTAATTGCAAGAGATAAAAAGGTTCTATTTGTTGCTGAAAAAAAGGCTGCTTTGGATGTTGTTTATAAATCAATCCAAAATCTTGGATTTGCTAATTTTATTTTGCTTATTCATAGCAATAACAGCAATAAAAAAGATTTTTCTAATGATTTGTATGAAACATTGCAAGACGGTCAAAAATTTAAAAAAGTAAGTGATGAAGTTATTGCTAATAATGATTATTTATATAGTGATTCATTAGAAAAAATGAATGATTTTGTTAGAAAGATATTATCAAATAGAAAACCACTAGGTCGCTCATTATATTTAATGATTGGTGATTATCAAAAATTACTAGCCAATACAAAAGGTATAAATTTTGATATTAGAGATTTTGAAAAAATGGATAATGAACAGTTTGTTAAAATTCAAAGTAGTTTAAATAAGTTTAATTTAAACTATAAAAATATAAATTTTAACGCTAAGGAAAGTTTATGA", "aa": "MDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARDKKVLFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIRDFEKMDNEQFVKIQSSLNKFNLNYKNINFNAKESL", "aa_hexdigest": "5b1b39d41942b3670129690779bbd336", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 300640, "stop": 301998, "observations": { "mutation": [ 300945, 301746 ], "stop": [ 300945, 301746 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024114E65", "query_cov": 1.0, "subject_cov": 0.4009584664536741, "identity": 0.98, "score": 477.0, "evalue": 2.3e-164, "gene_start": 1, "gene_end": 251, "reference_start": 229, "reference_end": 479, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQNWKIVDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEFLYSEEEVDNIVSPKGYYHCLMTNSSQEAAIQSAIKGNSFIIQGPPGTGKSQTIANIISELIARNKKILFVAEKKAALDVVYKSIQNLGFANFILLIHSNNSNKKDFSNDLYETLQDGQKFKKVSDEVIANNDYLYSDSLEKMNDFVRKILSNRKPLGRSLYLMIGDYQKLLANTKGINFDIRDFEKIDNEQFVKIQSSLNRFNLNYKNINFNAKESLWYGLSILDVKLQDLENIKEISSHLINEIQSLINFLDLEIPNNYQPYIKNSNYSLFNSFFKIFSNIQDIDINPKYLEIFSYNKTNATQTLNEILLQKEKIQPDIDWILSRYNQNIFNSEIYKNLDFVKSRNNIFKRIFSKRYRAIKKN" }, "paralog": false, "identity": 0.9668141592920354, "score": 859.0, "evalue": 2.68e-317, "description": "Internal stop codon at 300945, 301746. Nonsense mutation around 300945, 301746.", "psc": { "uniref90_id": "UniRef90_UPI0024114E65", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024114E65", "UniRef:UniRef50_UPI0024114E65" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024114E65", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024114E65" ], "product": "DUF4011 domain-containing protein" } }, "id": "GLJLFKCOHC_539", "locus": "GCA013368035_00523" }, { "type": "cds", "sequence": "contig_1", "start": 301744, "stop": 301986, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTTACCTGTTTCAATTGAAAGTGAAAACGGCTTGCAAAATAAAAAAATAATTATTCAAAGCAATGATTTCGTTGTAAATAATTCATTGATAAAGAAATTAAATGATGATTTTGGACTTGATATTAAATTTGAAGCATTTGATAATATTGAAGGTTCTCTATATGATAAATATACACAATACATTGAGTTTATAAATCAAAAAATGAATTCGTTCAATGATTCTATAAAACAAAATTGA", "aa": "MFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNIEGSLYDKYTQYIEFINQKMNSFNDSIKQN", "aa_hexdigest": "d5c575072be77ed8d283247270e7fdac", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9330.384899999995, "isoelectric_point": 4.5470853805542 }, "id": "GLJLFKCOHC_540", "locus": "GCA013368035_00524" }, { "type": "cds", "sequence": "contig_1", "start": 302023, "stop": 302382, "strand": "-", "frame": 2, "gene": null, "product": "DUF4011 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4", "UniRef:UniRef90_UPI00280BA0F4" ], "nt": "ATGGAAAATAAAGAATTAATTACAAAAACTTTGGCATATGAGTCTTTAAAGAATAAGCTATTAGACACAACATTAAGAAATATATTGATAAATTATAAAAAAAATAAATCATCAACTATAACAATTGAAAAGGAAGACATAAAATCAATTTTAGATAAAATTGAAAATAGAAGTGAATTTAAATTTATTGGTTCTCAAGATTCTGATGATGACATTGAAGTTGAAAGCAATGAATTTTTGTCCTCTGCTTCTCAAAAAGAATTATTAGCAATATTGTCGTTATTGCAAAGAAAGCAAAAGACTTTATTAGAAGAACAAGGAATTAATATTCTATATTTGGCTTTTGGTGGGCTTCAATGA", "aa": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLAFGGLQ", "aa_hexdigest": "e752a6132dc06b1414a19947b29d0368", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 301720, "stop": 302382, "observations": { "mutation": [ 301746, 302025 ], "stop": [ 301746, 302025 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280BA0F4", "query_cov": 1.0, "subject_cov": 0.4473684210526316, "identity": 0.992, "score": 211.0, "evalue": 4.75e-67, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MENKELITKTLAYESLKNKLLDTTLRNILINYKKNKSSTITIEKEDIKSILDKIENRSEFKFIGSQDSDDDIEVESNEFLSSASQKELLAILSLLQRKQKTLLEEQGINILYLSFGGLQWKDKTEKNVYSPLLFLPVSIESENGLQNKKIIIQSNDFVVNNSLIKKLNDDFGLDIKFEAFDNAEGSLYDRYSQYIEFINQKINSFNDSIEQDWKIIDEASLSAFRFSSMDIYLDVDKNKDNIINSEIMNAMTGINNVFSSEYLYSE" }, "paralog": false, "identity": 0.9545454545454546, "score": 405.0, "evalue": 8.19e-146, "description": "Internal stop codon at 301746, 302025. Nonsense mutation around 301746, 302025.", "psc": { "uniref90_id": "UniRef90_UPI00280BA0F4", "product": "DUF4011 domain-containing protein", "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280BA0F4", "UniRef:UniRef50_UPI00280BA0F4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00280BA0F4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00280BA0F4" ], "product": "DUF4011 domain-containing protein" } }, "id": "GLJLFKCOHC_541", "locus": "GCA013368035_00525" }, { "type": "cds", "sequence": "contig_1", "start": 302623, "stop": 303354, "strand": "-", "frame": 2, "gene": "tpiA", "product": "Triosephosphate isomerase", "db_xrefs": [ "COG:COG0149", "COG:G", "EC:5.3.1.1", "KEGG:K01803", "RefSeq:WP_036432897.1", "SO:0001217", "UniParc:UPI0005138FF4", "UniRef:UniRef100_A0A454C9S4", "UniRef:UniRef50_D1J827", "UniRef:UniRef90_D1J827" ], "nt": "ATGAAATATTTAATTGGTAATTTAAAAATGAATCTATCTTATTTAGAAAGTCAAAATTATTTAAATGAACTACAATCATTAAATAAAATTAAAAATTTTAAAAATGTAATAGTTGGAGCGGCTTTGTCATTCGATGCAATGTCTCTTTCTTTACAATATAATAAAAGAAATTTCTTGTTTGGAGTTCAAAATATTTTTTATAAAGAAAAAGGTGCATACACTGGGGAAGTTTCATTTAGATCAGCAGTTGAATTGAACATTGACTTTGTTTTAATAGGACATAGTGAACGTAGAAAATTATTTAATGAAACTGATCAATTGATTAATTTAAAAATTAAACAAATTGAAAAAACTAGCATTATTCCTATTTTGTGTATAGGGGAAGATCAAGAAAATTGTAGCGATAAAGTCTTTGAAGATATTATAAAAAACCAAATATTAAAAGCTTTAGAAGACGTCGATGTTTATAATCGTTTAATTATTTCATACGAACCTACTTATTGCATTGGAAATGGAAAAATTCCTCAAAATAACCATATCGAAAAAGCAATATCTGTAATTAAAAAAATTACTAATAATCAAGTTCCAGTGTTGTATGGTGGTTCAGTTTCACAAAAAAATATTGAAAAATTGCTTGAAATTAAGGATTTGGATGGTTTTTTAGTTGGCGGAGCAGCATTAGATGCTCAAGGTTTTTTAGAAATGGGTTCAATAATAGAAAAAAGCAACTAG", "aa": "MKYLIGNLKMNLSYLESQNYLNELQSLNKIKNFKNVIVGAALSFDAMSLSLQYNKRNFLFGVQNIFYKEKGAYTGEVSFRSAVELNIDFVLIGHSERRKLFNETDQLINLKIKQIEKTSIIPILCIGEDQENCSDKVFEDIIKNQILKALEDVDVYNRLIISYEPTYCIGNGKIPQNNHIEKAISVIKKITNNQVPVLYGGSVSQKNIEKLLEIKDLDGFLVGGAALDAQGFLEMGSIIEKSN", "aa_hexdigest": "f072e141c61a95602ea3d9cf508ba5ec", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0005138FF4", "ncbi_nrp_id": "WP_036432897.1", "uniref100_id": "UniRef100_A0A454C9S4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005138FF4", "RefSeq:WP_036432897.1", "UniRef:UniRef100_A0A454C9S4" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9S4", "uniref90_id": "UniRef90_D1J827" }, "psc": { "uniref90_id": "UniRef90_D1J827", "gene": "TpiA", "product": "Triosephosphate isomerase", "ec_ids": [ "5.3.1.1" ], "uniref50_id": "UniRef50_D1J827", "cog_id": "COG0149", "cog_category": "G", "kegg_orthology_id": "K01803" }, "pscc": { "uniref50_id": "UniRef50_D1J827", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J827" ], "product": "Triosephosphate isomerase" }, "genes": [ "tpiA" ], "id": "GLJLFKCOHC_542", "locus": "GCA013368035_00526" }, { "type": "cds", "sequence": "contig_1", "start": 303372, "stop": 303590, "strand": "-", "frame": 3, "gene": null, "product": "DUF3899 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828", "UniRef:UniRef90_D1J828" ], "nt": "ATGCGTCGCGCAAAAGACAAATTTTATTCAGGAATGACAATTCATCAAAAGGATGTTATTATTGCTAAAGAACGCCAAGAATTTGAACGTGAAAGACTCAAAGCTGAAAAGCAAAGAAACTATCAATCAATTAATAATTTATCATTTCTTTTATTAATATTATATGGATTAATTATATTGATTATATTGATTCCATTTTTAGCATTAAAAATAGTTTGA", "aa": "MRRAKDKFYSGMTIHQKDVIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALKIV", "aa_hexdigest": "5775a137310fe2c7a9390f485f205035", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 303372, "stop": 303890, "observations": { "mutation": [ 303635, 303830, 303842 ], "stop": [ 303635, 303830, 303842 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J828", "query_cov": 1.0, "subject_cov": 0.41379310344827586, "identity": 0.972, "score": 123.0, "evalue": 3.4e-34, "gene_start": 1, "gene_end": 72, "reference_start": 102, "reference_end": 173, "reference_sequence": "MTLKQKNFRNQKKSISYWKNAWNKATISYFFVSLVIYIALIFIVRYSKKSIDGQYVHSWQNSLTVSMIFAITINFIIVVYRKGMGKWIVNPIANLIRNRIIMRRAKDKFYSGMTIHQKDIIIAKERQEFERERLKAEKQRNYQSINNLSFLLLILYGLIILIILIPFLALRIVW" }, "paralog": false, "identity": 0.9651162790697675, "score": 319.0, "evalue": 6.42e-114, "description": "Internal stop codon at 303635, 303830, 303842. Nonsense mutation around 303635, 303830, 303842.", "psc": { "uniref90_id": "UniRef90_D1J828", "product": "DUF3899 domain-containing protein", "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J828", "UniRef:UniRef50_D1J828" ] }, "pscc": { "uniref50_id": "UniRef50_D1J828", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J828" ], "product": "DUF3899 domain-containing protein" } }, "id": "GLJLFKCOHC_543", "locus": "GCA013368035_00527" }, { "type": "cds", "sequence": "contig_1", "start": 304012, "stop": 304215, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGATGCAGAAATTAATTCAAATAAAATATTTCAAAAACCAGATGGCCAAGATTTTATTGAAAAATTTATAAACCCATATATTAATAAATTAGTTAATAACGAAAAAGACAGTTCATATACAAATTTCAAACAAGTTGAGCATGATAAAAATGAATTTAACGAAATTTATCACAAAAAAATAAATTCACTAATCAAATAA", "aa": "MADAEINSNKIFQKPDGQDFIEKFINPYINKLVNNEKDSSYTNFKQVEHDKNEFNEIYHKKINSLIK", "aa_hexdigest": "731d23d97f99171efb2278ca6f56d333", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7978.824699999997, "isoelectric_point": 6.059400367736816 }, "id": "GLJLFKCOHC_544", "locus": "GCA013368035_00528" }, { "type": "cds", "sequence": "contig_1", "start": 304231, "stop": 305115, "strand": "-", "frame": 2, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2", "UniRef:UniRef90_A0A454C9N2" ], "nt": "ATGAGAAAAAAATTACTTTATATATTACCGTTATCTTCAACTATTTTAATAACCCCATTAGTTGCAGCTTCTTGTTTTAAAAAACCTAAGCAACCTAAACAAAATATAGAAACCACACAAACTAAAGATGAAAATAAACAAAAATCTGAAGAATTATTAAAAAAATTTAATGATGCAAAAACTGATCTTTTTAGTTATTTAAAAGATAACTTTTTGATAGAAGGAGCTGAACAATTTAACACCTTAAAAGAAGTTCAGGATGTCAAGAAATTATTGGAATCTTTTAATAAAGAATCCTCTGAATCATCAATAGAAAGTTTAAATGAAAAAATACAAAAAATAAACGTTGCTAAACAAAAAATACTTAAAATTCAACCACTTTTAAAAGACTTAACAAAAATTGATAATTTAGTAAACGAATTAAATAATCTTGAAAATAAAATTAAATTAGATGCAGACGATTCTAAAATAGATAAATTAATAAGAAACGAATTAAAATCAATTGCTGATAATTTATCTTTAACTTTAAAGGCAATTAATCTAGAAGCATTATTGAATAATATTAATAGCAAAGATATTGCCAGTTATTTAAATAAATTGCAAGAAATTAAGCAAATAACCTCGCTTAGAATTCAAAAAACTAAATTTATTAATTTGGCATTGCAACAAAAATTCCAAGACCAAATAATTGAAAATATTATCAATAAAATTGCAAAAAACAATTTAAAAGAAAAATATATACCATTAATAAATCAATATCAATATTTTAAAGGACAAATTATTGAAGAAATTAACAAGTGTCATGCAAATGATAATTTAGCAGAATCTTTAAATGAAAAAATTAATTCTATAAGTCAAAAGTTAGCATTAAACGAGTCTATTTGA", "aa": "MRKKLLYILPLSSTILITPLVAASCFKKPKQPKQNIETTQTKDENKQKSEELLKKFNDAKTDLFSYLKDNFLIEGAEQFNTLKEVQDVKKLLESFNKESSESSIESLNEKIQKINVAKQKILKIQPLLKDLTKIDNLVNELNNLENKIKLDADDSKIDKLIRNELKSIADNLSLTLKAINLEALLNNINSKDIASYLNKLQEIKQITSLRIQKTKFINLALQQKFQDQIIENIINKIAKNNLKEKYIPLINQYQYFKGQIIEEINKCHANDNLAESLNEKINSISQKLALNESI", "aa_hexdigest": "774d7150501f1bbf2d6114fd4ee39d7d", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A454C9N2", "query_cov": 1.0, "subject_cov": 0.8010899182561307, "identity": 0.9179999999999999, "score": 428.0, "evalue": 4.31e-148, "valid": true, "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9N2" }, "pscc": { "uniref50_id": "UniRef50_A0A454C9N2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9N2" ], "product": "Lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_545", "locus": "GCA013368035_00529" }, { "type": "cds", "sequence": "contig_1", "start": 305330, "stop": 305953, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175949859.1", "SO:0001217", "UniParc:UPI00158C47FA", "UniRef:UniRef100_UPI00158C47FA", "UniRef:UniRef50_D1J831", "UniRef:UniRef90_D1J831" ], "nt": "ATGAGAAATAAAATTCGCTTATTAATACTAAGTGCCGTTGCAACTTCAGTTGTTGCAATGCCATTAGTTGCAGCAGGATGCAAAACAACAAATGATAGTCAAGATTCTACAACCACAGGTGAAGATTCGACTGGACAATCTCAATCTGGCGACATTATAGATCCTAATAAGAGAGCAAAAGGTCAATTAGGCCAAAGCCAATTGTCATATATTAGAGACCAATTTGGTTTTACTCTAACTAATGAAGGTAAAGAAATATTAAATGACCCTGCACGCGGAACAAAAGAATTACAAAGAATTTTAAAAGAAATAATCAATAAGTATAAATCTATTGATGAACAAATTGAAATCAACATAAGAAATAAACAAACTAGTGGAATAAATTCGCAAGTTTATAAAGCATATAAAAATGTACTGAGTGATGCAGATTTTGTAAAATATTTTAAGGTTACATTGCCTAAGATACTTATTCCGTTTGGCCACCCCATTTCAATACAATTAAAATCAGAGGGAAGATTAAGGGATTTGCCGGTAATTTCTTATAGTGTAATATGCCCTGATAAATCAAATAATGGAATTTTTAGTAGTGATGGTGATAGCGAAGTACCACTTGAAATTGATTAA", "aa": "MRNKIRLLILSAVATSVVAMPLVAAGCKTTNDSQDSTTTGEDSTGQSQSGDIIDPNKRAKGQLGQSQLSYIRDQFGFTLTNEGKEILNDPARGTKELQRILKEIINKYKSIDEQIEINIRNKQTSGINSQVYKAYKNVLSDADFVKYFKVTLPKILIPFGHPISIQLKSEGRLRDLPVISYSVICPDKSNNGIFSSDGDSEVPLEID", "aa_hexdigest": "a426ab775390192f48092a333f4aa6f0", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00158C47FA", "ncbi_nrp_id": "WP_175949859.1", "uniref100_id": "UniRef100_UPI00158C47FA", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C47FA", "RefSeq:WP_175949859.1", "UniRef:UniRef100_UPI00158C47FA" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C47FA", "uniref90_id": "UniRef90_D1J831" }, "psc": { "uniref90_id": "UniRef90_D1J831", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J831" }, "pscc": { "uniref50_id": "UniRef50_D1J831", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J831" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_546", "locus": "GCA013368035_00530" }, { "type": "cds", "sequence": "contig_1", "start": 305955, "stop": 306503, "strand": "-", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175949860.1", "SO:0001217", "UniParc:UPI00158E40EF", "UniRef:UniRef100_UPI00158E40EF", "UniRef:UniRef50_D1J832", "UniRef:UniRef90_D1J832" ], "nt": "ATGAATAAAAAGATTACACTATTTTGTTTACCAATAGCTTTAATAATTCCAACAATTGCAGCAGTTTCATGCCAAAAATCCTCTAATTTAAAAGCCAAAAATGATTTATTAAAAACCAAGATTATTGAAGGAATTAATTGCCCATGTCATACTAAATATAATGTTTTATCTAATAATAATCAAAACATGTCTAGTACTATGCTTGCAGCAATTTATGATAGTTTTAATTTTGAATCCAAAATACCGCTTAATCAAGAGAATAAGAAATTTTTATTCAAAATCATTGATAAACTCAATGATAAATACAAAAAAGATGATCAAAAGTCAAACATATTCAGTGATGAGTTATTTTTAAAATATTTTAAAATTTCATTGCCTGATTCGAAATACTTATTAAATCATACATTAAAATTAGAACTTGGATATGGTGATACAAATTTAAAATCAGATACTATATTTATAAAATATAGTATAATATGTAAATTAAGAAGTAGCCAATTTTTACAAGTTATTGAAGATGAACGATATATTTATATTCAGGGGGAATAA", "aa": "MNKKITLFCLPIALIIPTIAAVSCQKSSNLKAKNDLLKTKIIEGINCPCHTKYNVLSNNNQNMSSTMLAAIYDSFNFESKIPLNQENKKFLFKIIDKLNDKYKKDDQKSNIFSDELFLKYFKISLPDSKYLLNHTLKLELGYGDTNLKSDTIFIKYSIICKLRSSQFLQVIEDERYIYIQGE", "aa_hexdigest": "972dddea9d1863c93567ba7917413f62", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158E40EF", "ncbi_nrp_id": "WP_175949860.1", "uniref100_id": "UniRef100_UPI00158E40EF", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E40EF", "RefSeq:WP_175949860.1", "UniRef:UniRef100_UPI00158E40EF" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E40EF", "uniref90_id": "UniRef90_D1J832" }, "psc": { "uniref90_id": "UniRef90_D1J832", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J832" }, "pscc": { "uniref50_id": "UniRef50_D1J832", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J832" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_547", "locus": "GCA013368035_00531" }, { "type": "cds", "sequence": "contig_1", "start": 306503, "stop": 307006, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_036438522.1", "SO:0001217", "UniParc:UPI000512E14A", "UniRef:UniRef100_A0A454C9K7", "UniRef:UniRef50_D1J833", "UniRef:UniRef90_D1J833" ], "nt": "ATGAAAAAAATAATATTATCTATAGGGGCAGTTCTAGTTTTAGCTTCGCCAATAATTTCAGCATCTTGTGTTTCAACAGATGAAAATACAGCAAAAGCAAATGAAATTTACACTAATAAAAGTGGAATTTTTAATTCTTCTCAATTAGAAGCAATTAAAAATGATTTTGTTTTCGAATTAACTGAACAGAGCAAAATGCTAAAAAATAATTATGGCAATAAGGCGTTGGCAGATGAGCTAAAAAAGATTTGTAAAGATTATGAATTGCAAATTAATCTTAGCCCTTCTGAAAATAATAAATTAGCAGGTTTGAGGTTAATTAATAATGCAAACTTTTTAAAATTGTTTAAAGTGGTTAAGCCGAATTTAGGTGTAAATCACCAATTGATAATTAATTTTAAAATTGATAATAATAACAATATTTCATTAATCTATGACATATATTGCAAAGATGTAAAAACATATGATGCCAAAGATCAAGAAATAAAATTAGATTTAGAATAA", "aa": "MKKIILSIGAVLVLASPIISASCVSTDENTAKANEIYTNKSGIFNSSQLEAIKNDFVFELTEQSKMLKNNYGNKALADELKKICKDYELQINLSPSENNKLAGLRLINNANFLKLFKVVKPNLGVNHQLIINFKIDNNNNISLIYDIYCKDVKTYDAKDQEIKLDLE", "aa_hexdigest": "892330127ef8a105eaa97d96e0769221", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000512E14A", "ncbi_nrp_id": "WP_036438522.1", "uniref100_id": "UniRef100_A0A454C9K7", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512E14A", "RefSeq:WP_036438522.1", "UniRef:UniRef100_A0A454C9K7" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9K7", "uniref90_id": "UniRef90_D1J833" }, "psc": { "uniref90_id": "UniRef90_D1J833", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J833" }, "pscc": { "uniref50_id": "UniRef50_D1J833", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J833" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_548", "locus": "GCA013368035_00532" }, { "type": "cds", "sequence": "contig_1", "start": 307016, "stop": 307864, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834", "UniRef:UniRef90_D1J834" ], "nt": "ATGGTAAACATTTTTAAGCCATTGATTCCAAAAAGTGTTCATAAAAATAGTCTTGACTTTATTGACAATAGTTATTATCTTGCAAACAAAAAAATATTAAACGATCTTCAATCAAGCAATCTTGATTACAAATTAAAATCAGCTTATTTTCAAGAAATATCTAAACAACTAAATGATATAATCAAAAAGTTTTATGAACAACATCCAGATGCTGCTGATATTGAATTTGATATTCCACTAAATTTTTTGAATTTGCAATTTAATAACCAAAGGCAAAATGTTTTGGAACATATCAAAACAATATTTAAAGCAGTAAATAAACATTTAAAGCCAAATTTTGTAGTTATTGATGATTTAGCAAAATATGACTTGTATTTTAATCAAGATGCTTCAATATATTGCAATAATAGCTTTGAACTTTTAAAATCAAATACTAAAGAATTTATAGCAACAGCTATTGCTAAAAACTATAAATTGTTTTTAATATTTAATAAAATTTCTAGCTTAACTAAAAGCAATAGTTTTATTCTAGAAAATACCAAAAAAATATTAAATGAATTAAATAAAACAAAACTAATTACAAATAATGAAATTTTTAACAAAAATTTAGAAGATTACAACAAAAATTTCTTTATAATAAAATCAATTATATTAAAATACTTAGAAAATATCTCTTACCAAGACCAATTAAATATAATTAATGATATTAACAGTTTACTTTCTTATACCTTTTCATTTGAAAATATCATTAAAACTGTTGGTTTTTCAAAAGTAATATACCAAAAATTTATTAATAAACCAATTAGTTATGACGGGTTGTCTTACTTGCAAGATATTGTAATAGAATAG", "aa": "MVNIFKPLIPKSVHKNSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFFIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE", "aa_hexdigest": "4790524787c669ca76034965b803accf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 307016, "stop": 308164, "observations": { "mutation": [ 307942, 308131 ], "stop": [ 307942, 308131 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J834", "query_cov": 1.0, "subject_cov": 0.6482758620689655, "identity": 0.993, "score": 541.0, "evalue": 5.6e-192, "gene_start": 1, "gene_end": 282, "reference_start": 154, "reference_end": 435, "reference_sequence": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITINWMPTANKKFFNDSFSMLAYGLKANQLNENTNINKILKSKNIAFLLLIHQLINQYGMLANQGDIWTSQAPSDLDISSFDSGINYHVLKDSMVNIFKPLIPKNVHENSLDFIDNSYYLANKKILNDLQSSNLDYKLKSAYFQEISKQLNDIIKKFYEQHPDAADIEFDIPLNFLNLQFNNQRQNVLEHIKTIFKAVNKHLKPNFVVIDDLAKYDLYFNQDASIYCNNSFELLKSNTKEFIATAIAKNYKLFLIFNKISSLTKSNSFILENTKKILNELNKTKLITNNEIFNKNLEDYNKNFFIIKSIILKYLENISYQDQLNIINDINSLLSYTFSFENIIKTVGFSKVIYQKFINKPISYDGLSYLQDIVIE" }, "paralog": false, "identity": 0.9842931937172775, "score": 741.0, "evalue": 5.98e-273, "description": "Internal stop codon at 307942, 308131. Nonsense mutation around 307942, 308131.", "psc": { "uniref90_id": "UniRef90_D1J834", "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J834", "UniRef:UniRef50_D1J834" ] }, "pscc": { "uniref50_id": "UniRef50_D1J834", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J834" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 33135.69390000005, "isoelectric_point": 8.758884239196778 }, "id": "GLJLFKCOHC_549", "locus": "GCA013368035_00533" }, { "type": "cds", "sequence": "contig_1", "start": 307940, "stop": 308128, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCGACTGCAAATAAAAAATTTTTTAATGATAGTTTTTCAATGTTAGCATACGGTTTAAAAGTAAACCAATTAAATAAAAATACGAATATAAATAAAATATTAAAATCTAAAAATATTGCTTTTTTATTATTGATTCACCAATTGATTAATCAATATGGAATGTTGGCTAACCAAGGTGATATTTGA", "aa": "MPTANKKFFNDSFSMLAYGLKVNQLNKNTNINKILKSKNIAFLLLIHQLINQYGMLANQGDI", "aa_hexdigest": "f1381ebe0ab783630d10edc6e3149ccc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7057.2233, "isoelectric_point": 9.903326988220215 }, "id": "GLJLFKCOHC_550", "locus": "GCA013368035_00534" }, { "type": "cds", "sequence": "contig_1", "start": 308129, "stop": 308323, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATATCAAAAAATACTTTTGGTATGCAAATGTATCATGCATTTTTGCAAAATATTGTAACTACATTGAATTTTGACGATCTTGACATAAATCAACAACAAGAAATTTTGCATAAATATAATGATTTTAATATAAGTTATACTCGTAATGTTTCTAAAAATAAGTTGAGTAATAATATTACCATTAATTGA", "aa": "MPISKNTFGMQMYHAFLQNIVTTLNFDDLDINQQQEILHKYNDFNISYTRNVSKNKLSNNITIN", "aa_hexdigest": "3364ab9bd125409e701de2e7bc69a378", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7553.4361, "isoelectric_point": 6.698270606994629 }, "id": "GLJLFKCOHC_551", "locus": "GCA013368035_00535" }, { "type": "cds", "sequence": "contig_1", "start": 308882, "stop": 309445, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAACATTCTTATTTTAAGTTCAATGTTAGCATTATTTACAATGACATCTGCGATATCAATATCTTGTAAATTTAATAATTTTAATGAAGACAAGAAAAAATATATTCAAAAGCTTAATATTGCAAATCCTTATTTAGAATATGGCAGTTCAATACCAAATTTTAAAAACCAAAATTTAAAAGAAATAAATTTAATGACAGGAGCCAAAGTTCTTAGAATTTATTCAAAAAATCAACCTAAAATAGACTTTAGGGATAATATAGTTTTGCGCCCGACTGAATTAAGTTATAAATTTGAATATGGTAATAGTATTCAAATAAATGATAAACTTTTCAATAGTGATGATAAACTAAATGTTGACTATCAAAACATTGAATTGAATAAGCAAGATCCCAACAAATATTACCCCAGCAAAGACAAAGGCAATGGTTTTAATAATCCATATTTGTTCGTTCCTTCAAAAAATTCAATGTCTATAAATAGCAAAGAATTCATTGAATCTCTAAGAAAAGCAAAAAATATTTCAATCAGTATAAATCAAAGAAAAAATTATTGA", "aa": "MKKNILILSSMLALFTMTSAISISCKFNNFNEDKKKYIQKLNIANPYLEYGSSIPNFKNQNLKEINLMTGAKVLRIYSKNQPKIDFRDNIVLRPTELSYKFEYGNSIQINDKLFNSDDKLNVDYQNIELNKQDPNKYYPSKDKGNGFNNPYLFVPSKNSMSINSKEFIESLRKAKNISISINQRKNY", "aa_hexdigest": "c24ba4b9d0febaf9f5675b8b56311fc9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21780.710200000005, "isoelectric_point": 9.612832450866701 }, "id": "GLJLFKCOHC_552", "locus": "GCA013368035_00536" }, { "type": "cds", "sequence": "contig_1", "start": 309456, "stop": 309635, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCATTAGGTGGAGGAATTATTACATTTATGTCCATATTTGAATTGGTTCCGGAATTTATCCATGTTCGAAATCAATCCCCAAAAGTTTTATATACTTCATTTGCTTTATTTAGTTTAGGACTAGTATTAACTATAGTTATTTTATGTTTTCACACTCATACAGCAACGGCATTTTAA", "aa": "MALGGGIITFMSIFELVPEFIHVRNQSPKVLYTSFALFSLGLVLTIVILCFHTHTATAF", "aa_hexdigest": "df9eba0a2f797faf690ad37dba9b4957", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6514.716899999998, "isoelectric_point": 6.806889915466309 }, "id": "GLJLFKCOHC_553", "locus": "GCA013368035_00537" }, { "type": "cds", "sequence": "contig_1", "start": 309651, "stop": 309932, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAATGAGTCATAGAATTATTGATGGACTATTTTTGGGATATAATGTTTATACATTAACATCAATTGGTGGAACATATAAAGCAAATATTCCCTTAATAATAACATTTAATATTCATATATTACTTGAAATTTTAGTTGTTTATTATCGTCAAATTCAATATGGAGAAAAGAAATCAAAAGCCATACTATACAACTTCTTAACATTTTTATTAATTATCCCATTTATGTTAATTGGCGGATTGCTTGGTCAATATATTGAACATAGTAAAGTGTTTTGA", "aa": "MLMSHRIIDGLFLGYNVYTLTSIGGTYKANIPLIITFNIHILLEILVVYYRQIQYGEKKSKAILYNFLTFLLIIPFMLIGGLLGQYIEHSKVF", "aa_hexdigest": "6c261c62e31d345c939dfab4a8d16dc6", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10745.78719999999, "isoelectric_point": 9.260512733459471 }, "id": "GLJLFKCOHC_554", "locus": "GCA013368035_00538" }, { "type": "cds", "sequence": "contig_1", "start": 310173, "stop": 310400, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATAGGAACAAGTGCATTTAACGGAAATATAACTTTAGCAACATTTGTAAATCTAATAATCTATATTTTAATATTATTGGGCGTACCTTCATTAGTTTTGGGCGTAATTTCTATATTTAAATTAAAAATTACTGATAAGAAAAATATTTATATTTATGCATTTGCTACGGGAATGTTTCTAATGATTGGCACTGTAGGTTTCTTTAAAGAAAGTTTTGATTGA", "aa": "MFIGTSAFNGNITLATFVNLIIYILILLGVPSLVLGVISIFKLKITDKKNIYIYAFATGMFLMIGTVGFFKESFD", "aa_hexdigest": "2dc2ffb6a1c8af9c24258720723a8558", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8289.930899999998, "isoelectric_point": 9.158330345153807 }, "id": "GLJLFKCOHC_555", "locus": "GCA013368035_00539" }, { "type": "cds", "sequence": "contig_1", "start": 310481, "stop": 310948, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCTCACTTAGCTTTATGCCTTTAATTATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAGTTTTTTTGCATAGGTTAAGCATAGGTCAGATTGCTGCATTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGCGATATTAATAATTTTTTAAACCAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGA", "aa": "MKKKFYFNSILIASSLSFMPLIICSCQTKKDNYIIEGSNAYIDISKISRVFLHRLSIGQIAALHNMYKIFYYYDNSNKKQYYDSAYVNGQKLYLRNANKNVEYKLNFPIKPSWKQEISKFDNINIVKSNKPSDINNFLNQYSFDEVDSAGDVNDE", "aa_hexdigest": "8b6837c93bd8170c500ec0e8e42a4ca7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18136.416099999995, "isoelectric_point": 9.039644050598145 }, "id": "GLJLFKCOHC_556", "locus": "GCA013368035_00540" }, { "type": "cds", "sequence": "contig_1", "start": 311015, "stop": 311158, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAACTATTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGA", "aa": "MQTIIFRLIQDTELNYSIMKSKYLINSKRENVITQNLFKNKYIQASS", "aa_hexdigest": "e03fbba843d8b202d0a18682185b7014", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.493300000002, "isoelectric_point": 9.775808525085449 }, "id": "GLJLFKCOHC_557", "locus": "GCA013368035_00541" }, { "type": "cds", "sequence": "contig_1", "start": 311594, "stop": 312016, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATCTTTTTCAAAGCTTTAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCCGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACCCCATATTTTAAAACAAATCAAATTTTTAAAGCAATAGTAAGGGTATTTAAACTAAATGGTACATATAAAGATTATGTTTTAATATCTTCTAATTTTGATGATCACGGTCACAGATTAAAAGGTTTAATTACTAAAAATGCTTTGCCAAATGAACTTAAAGCTTCTGATATATACTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTGGATGATTTCATTGATAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGA", "aa": "MYLFQSFKNEPGFFKLAVPEYKKNEDKEYKIIDFEYTPYFKTNQIFKAIVRVFKLNGTYKDYVLISSNFDDHGHRLKGLITKNALPNELKASDIYSIRADSEPIKQGIKLDDFIDKNNKNSVFRYMLNESAKKLENIFEY", "aa_hexdigest": "334cd2664ba84e5911bb83b26f45ccc0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16604.766499999987, "isoelectric_point": 8.872735404968264 }, "id": "GLJLFKCOHC_558", "locus": "GCA013368035_00542" }, { "type": "cds", "sequence": "contig_1", "start": 312056, "stop": 312313, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGCCAAAGATCCATTCCAATTAAAATTATTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCACTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTACCAAATCAACCAATTGGAAGATTATATTTAAAGATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGA", "aa": "MLAKDPFQLKLLASYLNNYLLAYALENKAGEVFSGIKRIDLNIVKLPNQPIGRLYLKMDFVSYANNQDFKFKSKNEKIVKSVYLY", "aa_hexdigest": "90a334c80318eee6e758a6d23630ff18", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9882.500599999992, "isoelectric_point": 9.58440189361572 }, "id": "GLJLFKCOHC_559", "locus": "GCA013368035_00543" }, { "type": "cds", "sequence": "contig_1", "start": 312397, "stop": 312774, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCAATAAAATTGCTATCCTTGCTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCACGATAACGAAGACAAGGAAAATTTAGATTTTTCAAGTGATTTTAGTGGCAAAAAGCAAAAAAATGAATTCAATTTCAAAAATAAGATTTCTCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAACACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAATTGA", "aa": "MKKSIKLLSLLCTSTIISLPAISCTIKVNHDNEDKENLDFSSDFSGKKQKNEFNFKNKISQNIYDLYQNKIAILFNDVKTNYRGYKKIFGSLTRNLNTLRAKINSLRYEQSLKENKKILNDFYNN", "aa_hexdigest": "44f8f9321ebd60c40bb3a5603820618c", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14610.592599999982, "isoelectric_point": 9.645195579528806 }, "id": "GLJLFKCOHC_560", "locus": "GCA013368035_00544" }, { "type": "cds", "sequence": "contig_1", "start": 312793, "stop": 312990, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCTGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAAACAATTGATGACAGACTTGAAGGTAAAGACATTGATTTAGGTCAAACTCAACATGCATTACAAGCGGTTTGA", "aa": "MLKNNPFALYLFKYSLIFSDVDAVLADTNLVFETSEFLKYLKTIDDRLEGKDIDLGQTQHALQAV", "aa_hexdigest": "53ee0f7517b2a8fe14d8b8609555d331", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7441.420799999998, "isoelectric_point": 4.562545585632326 }, "id": "GLJLFKCOHC_561", "locus": "GCA013368035_00545" }, { "type": "cds", "sequence": "contig_1", "start": 313042, "stop": 313281, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTCGATAAAATTAACATTGAGGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTGAAAAAAAATAAACTTCAAAATTACGAAGAATTTCAAAAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAAATTGA", "aa": "MLDKINIEDDKNSHNHSHAIINLTYEMGLWHEMLKKNKLQNYEEFQKEYKIAKKHIIDNINNIDVNNNYLALKKVLDKN", "aa_hexdigest": "bfc1a613cb73e5b77bb4a8280c536c5a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9493.729199999989, "isoelectric_point": 6.965243339538574 }, "id": "GLJLFKCOHC_562", "locus": "GCA013368035_00546" }, { "type": "cds", "sequence": "contig_1", "start": 313285, "stop": 313419, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAAAATTCTTATTACAACTTATTAAATGATTCATTTTTTAGTAGAGCAAAAGAAATTTTAAAAATACTAAAAGAAAATTTGATAAGTATTGCTAAAATTGTGGGTTTAACTGATAAAATCAATTTAGACTAA", "aa": "MENSYYNLLNDSFFSRAKEILKILKENLISIAKIVGLTDKINLD", "aa_hexdigest": "d85ad7b0de9c87870631cca0b718f744", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5088.869700000001, "isoelectric_point": 6.1138521194457995 }, "id": "GLJLFKCOHC_563", "locus": "GCA013368035_00547" }, { "type": "cds", "sequence": "contig_1", "start": 313754, "stop": 313972, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_203282136.1", "SO:0001217", "UniParc:UPI00193A815F", "UniRef:UniRef100_UPI00193A815F", "UniRef:UniRef50_UPI001F2618CA", "UniRef:UniRef90_UPI00193A815F" ], "nt": "ATGCCCAAAAAAGCTAAAGGCTCAGGTCGTCCAAGAAAAAGAGATATTGAACAAGAGAACAAACTTCGTGAAAAAACTTTAAAAGAAATGTCAAGAGAACAACTCGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAATTAAAAAGAAGCATAATTTTCAATATCAATAA", "aa": "MPKKAKGSGRPRKRDIEQENKLREKTLKEMSREQLEYLITDIFYEEILKRNNSKNLEEVVEKIKKKHNFQYQ", "aa_hexdigest": "7dfc754054b44760aa18b330304a9547", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00193A815F", "ncbi_nrp_id": "WP_203282136.1", "uniref100_id": "UniRef100_UPI00193A815F", "db_xrefs": [ "SO:0001217", "UniParc:UPI00193A815F", "RefSeq:WP_203282136.1", "UniRef:UniRef100_UPI00193A815F" ] }, "ips": { "uniref100_id": "UniRef100_UPI00193A815F", "uniref90_id": "UniRef90_UPI00193A815F" }, "psc": { "uniref90_id": "UniRef90_UPI00193A815F", "uniref50_id": "UniRef50_UPI001F2618CA" }, "pscc": { "uniref50_id": "UniRef50_UPI001F2618CA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2618CA" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8824.072699999993, "isoelectric_point": 9.654479026794434 }, "id": "GLJLFKCOHC_564", "locus": "GCA013368035_00548" }, { "type": "cds", "sequence": "contig_1", "start": 314038, "stop": 314703, "strand": "+", "frame": 1, "gene": null, "product": "DDE-type integrase/transposase/recombinase", "db_xrefs": [ "RefSeq:WP_241863341.1", "SO:0001217", "UniParc:UPI001F51AC52", "UniRef:UniRef100_UPI001F51AC52", "UniRef:UniRef50_C4XED4", "UniRef:UniRef90_UPI001F51AC52" ], "nt": "TTGCCAGCCAAAAACAAGGCAATTCAATATGAAAAACAAATAAAAAAAAAGGGGGGGGGGGCTTTTATTGAATGCAAAGGAAGATTTAGAAGGGAAAGACTTTCACTTTTTTTAAAGAAAAAGTACCAAATTGATATAAACCCAAGGACTCTGGGCAGAATTATGAATAAATTGGGTTTGCTATGCAAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATAAATGATATATATGTTACCGATGTGACATATATTCCTTCACCCACTGATATTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTATGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGACTACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATATTTTCAAATTTTTTCAAAAAAGCAAAAAGTTTAACATTTAA", "aa": "MPAKNKAIQYEKQIKKKGGGAFIECKGRFRRERLSLFLKKKYQIDINPRTLGRIMNKLGLLCKIRRSKRKREIKNVNATFKNLANRDYDGQINDIYVTDVTYIPSPTDIIENHVYLSVLLHHKTKKIMSWNLSKYNDTNLVLKHFKLTKFPKKFIVHSDYGYQYSSKEFLDYIDSINGTISMSRVGNSLDNREVEYFFSILKTEIFSNIFKFFQKSKKFNI", "aa_hexdigest": "3a4fce3d597b13f2cd505502831522e4", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "ups": { "uniparc_id": "UPI001F51AC52", "ncbi_nrp_id": "WP_241863341.1", "uniref100_id": "UniRef100_UPI001F51AC52", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F51AC52", "RefSeq:WP_241863341.1", "UniRef:UniRef100_UPI001F51AC52" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F51AC52", "uniref90_id": "UniRef90_UPI001F51AC52" }, "psc": { "uniref90_id": "UniRef90_UPI001F51AC52", "product": "DDE-type integrase/transposase/recombinase", "uniref50_id": "UniRef50_C4XED4" }, "pscc": { "uniref50_id": "UniRef50_C4XED4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XED4" ], "product": "Integrase catalytic domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_565", "locus": "GCA013368035_00549" }, { "type": "cds", "sequence": "contig_1", "start": 315044, "stop": 315736, "strand": "-", "frame": 1, "gene": "ddaH", "product": "Dimethylargininase (N[G],N[G]-dimethylarginine dimethylaminohydrolase)", "db_xrefs": [ "COG:COG1834", "COG:E", "EC:3.5.3.18", "KEGG:K01482", "SO:0001217", "UniRef:UniRef50_D5E539", "UniRef:UniRef90_A0A448ZVQ2" ], "nt": "ATGTGTAATTGTAAAAAATGTAATGATTGCAAAGATTGCTGCAAATCATGTGAAAAACCTTTAAAAAATAAATTTAATAGTATTATTGTTAAAACCCCATGTGCTGCTATGGTAAATGGTATTTCATCAGCTGGCTTACATCCAGATTATGAACTTGCTAAAAAACAACACAAAGATTATATTGAAAAATTAAAGACTTGTGGTGTTGATATAACCGTTTTACCTTTAAATGAAAAATATCCAGACTCATGCTTCGTAGAAGATGCTGCTGTTATTATTCCAGGTGAACTAGCTATTTTAACTAATCCAGGTGCTAAAACACGTAATGGAGAAAAAGTTGAAATGCTTCCTGCATTAAAGAAATACTTTGATGATGGACACATTGCAAAAATTGAAGCTCCCGGAACTGTTGACGGGGGAGATGTTATGATGGTTGGAGATACATACTACATTGGTATGTCCGAAAGAACTAATCGTGACGGAATTAGACAATTCCACAATATATTAGCAAAAATTGGTAAGAAGTTAGTTCCAGTACCAATGACAGAAATGTTGCACCTTAAAACAGGAGTTAACTATCTTGAACATAATAACTTATTAATTGCAGGTGAATTTGTTAACTATCCTACATTTGATTCATTTAACAAAATTGTAGTTCCTGCCGATGAAGCATATGCCGCAAACTGTATTTGA", "aa": "MCNCKKCNDCKDCCKSCEKPLKNKFNSIIVKTPCAAMVNGISSAGLHPDYELAKKQHKDYIEKLKTCGVDITVLPLNEKYPDSCFVEDAAVIIPGELAILTNPGAKTRNGEKVEMLPALKKYFDDGHIAKIEAPGTVDGGDVMMVGDTYYIGMSERTNRDGIRQFHNILAKIGKKLVPVPMTEMLHLKTGVNYLEHNNLLIAGEFVNYPTFDSFNKIVVPADEAYAANCI", "aa_hexdigest": "11f0c92678f5d024184be076b0b4f83c", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A448ZVQ2", "query_cov": 1.0130434782608695, "subject_cov": 0.8090277777777778, "identity": 0.97, "score": 439.0, "evalue": 1.13e-154, "valid": true, "gene": "DdaH", "product": "Dimethylargininase (N[G],N[G]-dimethylarginine dimethylaminohydrolase)", "ec_ids": [ "3.5.3.18" ], "uniref50_id": "UniRef50_D5E539", "cog_id": "COG1834", "cog_category": "E", "kegg_orthology_id": "K01482" }, "pscc": { "uniref50_id": "UniRef50_D5E539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E539" ], "product": "Dimethylargininase (N[G],N[G]-dimethylarginine dimethylaminohydrolase)" }, "genes": [ "ddaH" ], "id": "GLJLFKCOHC_566", "locus": "GCA013368035_00550" }, { "type": "cds", "sequence": "contig_1", "start": 316102, "stop": 316335, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015884C7E", "UniRef:UniRef90_UPI0015884C7E" ], "nt": "TTGAAAATTAATGACAGCAAAATTAGCAATAGTGAAAAATTAATTTATAAGGCGAATATTTTATTGATTGAAAACACTAAAAAATTGTCAATATATTCAAATAAAAACCAAAATTATAAATTTGGTGAAATTATTGAAGTAGATGCTTGCCAAGATAGATTTTTTGTAACCAATGAAAAAATTCATATTTATCATGCAATTGATGCCAAAAGTGGTGCATTAGTTGCCTTGTGA", "aa": "MKINDSKISNSEKLIYKANILLIENTKKLSIYSNKNQNYKFGEIIEVDACQDRFFVTNEKIHIYHAIDAKSGALVAL", "aa_hexdigest": "dcd8923eaef98eca89c367173f2d82c0", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 315928, "stop": 316335, "observations": { "mutation": [ 316104 ], "stop": [ 316104 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015884C7E", "query_cov": 1.0, "subject_cov": 0.5703703703703704, "identity": 1.0, "score": 151.0, "evalue": 7.38e-46, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MKINDSKISNSEKLIYKANILLIENTKKLSIYSNKNQNYKFGEIIEVDACQDRFFVTNEKIHIYHAIDAKSGALVALWAEKEKTNKGYQKLFELNQKYLDQQNKTTIEIIKNQTKIYNELIEINQKINDFIWQKI" }, "paralog": false, "identity": 0.9851851851851852, "score": 265.0, "evalue": 5.63e-93, "description": "Internal stop codon at 316104. Nonsense mutation around 316104.", "psc": { "uniref90_id": "UniRef90_UPI0015884C7E", "uniref50_id": "UniRef50_UPI0015884C7E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015884C7E", "UniRef:UniRef50_UPI0015884C7E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0015884C7E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015884C7E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8820.068299999997, "isoelectric_point": 8.710339546203613 }, "id": "GLJLFKCOHC_567", "locus": "GCA013368035_00551" }, { "type": "cds", "sequence": "contig_1", "start": 316407, "stop": 316781, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J842", "UniRef:UniRef90_UPI0003657047" ], "nt": "ATGGCAAAAATTAATGGTTCAATTATTTCAGAATATGAAATTAAAAAGATTAAAAATTTAGATTTTATTTCCAAAAATTTGCAAGAAAAAACAATGTTATCAATTCCTACAATTTGTAGATATAAAAAAATTATTAAAGATAATATGCAAGGTAGCAAAATTAAAGTAAATATTAGACATAAAAACTTTAATAATCAAAATGCAAAAATAATAGAAAACGATTTTGCAAAAGACTTATTTTTGAAATATCAAATTGAAAATCAAAAAATAAATTCAAATGTTACTAAAAAATTTATTACTGTTAAAGATTTTTATGAAGACCTTAGTGAAGAAATAAAGTCAAAAATTTCGTTATCGTATTTATATAATGCTTGA", "aa": "MAKINGSIISEYEIKKIKNLDFISKNLQEKTMLSIPTICRYKKIIKDNMQGSKIKVNIRHKNFNNQNAKIIENDFAKDLFLKYQIENQKINSNVTKKFITVKDFYEDLSEEIKSKISLSYLYNA", "aa_hexdigest": "49bd56b5d6161a9f7cda8b9757364b64", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 316194, "stop": 316781, "observations": { "insertion": [ 316256 ], "deletion": [ 316340 ], "mutation": [ 316409 ], "stop": [ 316409 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0003657047", "query_cov": 1.0, "subject_cov": 0.6391752577319587, "identity": 0.976, "score": 234.0, "evalue": 8.86e-77, "gene_start": 1, "gene_end": 124, "reference_start": 1, "reference_end": 124, "reference_sequence": "MAKINGSIISKYKIKKIKNLDFISKNLQEKTMLSIPTICRYKKIIKDNMQGSKIKVNIKHKNFNNQNAKIIENDFAKDLFLKYQIENQKINSNVTKKFITVKDFYEDLSEEIKSKISLSYLYNAWLKHGFCSPYATHKIKRIAKNISNLKINDSKISNSEKLIYKANILLIENTKNCQYIQIKTKIINLVKLLK" }, "paralog": false, "identity": 0.9743589743589743, "score": 357.0, "evalue": 5.56e-128, "description": "Internal stop codon at 316409. Frameshift due to insertion around 316256. Frameshift due to deletion around 316340. Nonsense mutation around 316409.", "psc": { "uniref90_id": "UniRef90_UPI0003657047", "uniref50_id": "UniRef50_D1J842", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0003657047", "UniRef:UniRef50_D1J842" ] }, "pscc": { "uniref50_id": "UniRef50_D1J842", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J842" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14602.842199999981, "isoelectric_point": 9.561580085754393 }, "id": "GLJLFKCOHC_568", "locus": "GCA013368035_00552" }, { "type": "cds", "sequence": "contig_1", "start": 316925, "stop": 317152, "strand": "+", "frame": 2, "gene": null, "product": "helix-turn-helix transcriptional regulator", "db_xrefs": [ "RefSeq:WP_036432939.1", "SO:0001217", "UniParc:UPI0005132A01", "UniRef:UniRef100_UPI0005132A01", "UniRef:UniRef50_A0A133Y810", "UniRef:UniRef90_UPI0005132A01" ], "nt": "ATGGCACTAAGTTATAATAAATTGTGGAAACTTCTGATTGATAAAAATATTAATAAAACTGACCTAAGGAATATGACGGGAATTAGCCAATCTACCATGGCTAAACTCAATAAAGGTGAAAATGTGAATACTGAAGTACTTGAAAGAATCTGCATTGCTCTCGATTGCCAAATTTCTGAAATAGTTGAAGTAGTAAATAAAGAATGTGAGGAAAAAAATAATGAATAA", "aa": "MALSYNKLWKLLIDKNINKTDLRNMTGISQSTMAKLNKGENVNTEVLERICIALDCQISEIVEVVNKECEEKNNE", "aa_hexdigest": "e46fd0437588293be984912013c50546", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005132A01", "ncbi_nrp_id": "WP_036432939.1", "uniref100_id": "UniRef100_UPI0005132A01", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005132A01", "RefSeq:WP_036432939.1", "UniRef:UniRef100_UPI0005132A01" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005132A01", "uniref90_id": "UniRef90_UPI0005132A01" }, "psc": { "uniref90_id": "UniRef90_UPI0005132A01", "product": "helix-turn-helix transcriptional regulator", "uniref50_id": "UniRef50_A0A133Y810" }, "pscc": { "uniref50_id": "UniRef50_A0A133Y810", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A133Y810" ], "product": "HTH cro/C1-type domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_569", "locus": "GCA013368035_00553" }, { "type": "cds", "sequence": "contig_1", "start": 317145, "stop": 318389, "strand": "+", "frame": 3, "gene": null, "product": "50S ribosomal protein L11 methyltransferase", "db_xrefs": [ "RefSeq:WP_175949865.1", "SO:0001217", "UniParc:UPI0015887893", "UniRef:UniRef100_UPI0015887893", "UniRef:UniRef50_A0A3R9R7L9", "UniRef:UniRef90_UPI0015887893" ], "nt": "ATGAATAATTATAAAAGATTCTATGACGAAAAATGGAATTTTAAAGAAGGCAGTATGAAGGATTTGGTTCACGGTTTACATCCATATCCTGCAATGATGATGCCATTGATATGTAGAGAGATGTTAAATCAATATGGAAAGGGAAGCGAGACCGTATTTTTAGATCCTTATGTTGGCTCTGGCACAACCTTGGTTGAAGCACAATATTATGGTGCAAAAAAAGCGTATGGAGTTGATTTGAATCCATTAGCCATCTTAATTTCGAGGACAAAAACAGAAGAAATAGATTTAAAACAAATACGAGTTTATATGGATTCTTTCGATGAAACGATTAGTAATATTAGTGATGATGTTTCCGCACCTGAATTTTCTATTAGAGACTCATGGTTCAACAATAAAACGATAAGAGATTTAGCTAAAATCACCGCTTTTGTAAATTCAATAAAAGATTCTACAATAAATGATTTTTTCAAAATTTCTTTATCAGAAGTGATTAGAACGTCATCTGAAACAAGAAATGGCGAATTTAAGCTTTATAGAATGTCAAAAAAAGCGTTGGAAAAATTCAATCCTGAACCAATAAGAATATATAAGGAAGTGATTGAACGTAACTATGGAATACTAGAATCAATTAATTATAAGAGAAATACTGATATTATATTAATTAATGAATCAACTCTAAATTTAGAAAAGCACAATGAACTTAATCAAAAAGTTGATATTATAGTAACTTCGCCACCTTATGGTGATAGTCATACAACTGTTGCTTATGGACAATTCTCAAGATTGGCAAATGAATGGCTTGGATTTGATGGTGCCGGTTCCTTGGATAAAAGATTATTAGGAGGAAAAAAAGTCCAAAATAAGACTTTTGATATCCCAGAGCTAGATACGGCAATTGAAAAAATCAATGAAGCTGATAAAAAGTTTAATCGTTGTAGAGCTTGGGAAGTTGTTGCTTTTTATTCTGATTATGAAGACTCCATAAAAGCAGTTGATAAAACTGTGAAAAATGGAGGATACGTTGTTTATGTTGTTGGAAATAGAAGAGTAAGAAATATCGAGCTTCCTTTAGATGTAATAACCTATAAAATGTTTGAAAAGTTAGGATATTACCACGTAGTGACACATGTAAGAGACATTTTAAATAAAAGAATGCCATCGAAAGCTTCTCCGTCAAATGAAGCAGGGGAACAGATTCCAACAATGACAAGCGAATATATCGTTGTAATGAAAAAGAATTAA", "aa": "MNNYKRFYDEKWNFKEGSMKDLVHGLHPYPAMMMPLICREMLNQYGKGSETVFLDPYVGSGTTLVEAQYYGAKKAYGVDLNPLAILISRTKTEEIDLKQIRVYMDSFDETISNISDDVSAPEFSIRDSWFNNKTIRDLAKITAFVNSIKDSTINDFFKISLSEVIRTSSETRNGEFKLYRMSKKALEKFNPEPIRIYKEVIERNYGILESINYKRNTDIILINESTLNLEKHNELNQKVDIIVTSPPYGDSHTTVAYGQFSRLANEWLGFDGAGSLDKRLLGGKKVQNKTFDIPELDTAIEKINEADKKFNRCRAWEVVAFYSDYEDSIKAVDKTVKNGGYVVYVVGNRRVRNIELPLDVITYKMFEKLGYYHVVTHVRDILNKRMPSKASPSNEAGEQIPTMTSEYIVVMKKN", "aa_hexdigest": "89d788c8ff8cf9ad6ee201576a9637dd", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0015887893", "ncbi_nrp_id": "WP_175949865.1", "uniref100_id": "UniRef100_UPI0015887893", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015887893", "RefSeq:WP_175949865.1", "UniRef:UniRef100_UPI0015887893" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015887893", "uniref90_id": "UniRef90_UPI0015887893" }, "psc": { "uniref90_id": "UniRef90_UPI0015887893", "product": "50S ribosomal protein L11 methyltransferase", "uniref50_id": "UniRef50_A0A3R9R7L9" }, "pscc": { "uniref50_id": "UniRef50_A0A3R9R7L9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3R9R7L9" ], "product": "site-specific DNA-methyltransferase (cytosine-N(4)-specific)" }, "genes": [], "id": "GLJLFKCOHC_570", "locus": "GCA013368035_00554" }, { "type": "cds", "sequence": "contig_1", "start": 318698, "stop": 318904, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0007610F17", "UniRef:UniRef90_UPI0007610F17" ], "nt": "ATGGGGAAAAATATAATCTTTTATATGTCAAAGCTTCAGCTGATAAATGGACATATAACAAAATTAAAACATTTGCCTTTAAACTTAAATTTGGCAATGAAAACAAAAAAATTTTCTATTAGTAAAAATTCTTCTGGTAATATCGTCGAAGATGAGAAAACCGGTAAAAAAGAATTTTTTCATACACGAAAATTAAAGAAATCATAG", "aa": "MGKNIIFYMSKLQLINGHITKLKHLPLNLNLAMKTKKFSISKNSSGNIVEDEKTGKKEFFHTRKLKKS", "aa_hexdigest": "d30e254e7631b18352b621a19047905a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0007610F17", "query_cov": 0.8823529411764706, "subject_cov": 1.0, "identity": 1.0, "score": 115.0, "evalue": 1.2e-32, "valid": true, "uniref50_id": "UniRef50_UPI0007610F17" }, "pscc": { "uniref50_id": "UniRef50_UPI0007610F17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0007610F17" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7847.276799999997, "isoelectric_point": 10.298582649230955 }, "id": "GLJLFKCOHC_571", "locus": "GCA013368035_00555" }, { "type": "cds", "sequence": "contig_1", "start": 319186, "stop": 319944, "strand": "-", "frame": 2, "gene": null, "product": "MvaI/BcnI family restriction endonuclease", "db_xrefs": [ "RefSeq:WP_175949866.1", "SO:0001217", "UniParc:UPI00158E1D4B", "UniRef:UniRef100_UPI00158E1D4B", "UniRef:UniRef50_UPI00159302B7", "UniRef:UniRef90_UPI00159302B7" ], "nt": "ATGACAGATAATTATGAAATTGAATTAATAAATTTTTTTAAGGATATAAAAAGTCGAGAATGGATTGAGACAACTAGGCACGGTGATCAATGCTTAGGCAATGCTTTCGAAGATTTGTTAGGTGTTAAAGAAAACAACAAAAATGAAGCTGATTTTCATGGCATTGAATTGAAAACGCATAGAAGTATTACTTCTTCTATGGTTTCTCTGTTTTCAAAATCTCCTTCCCATCCAAAAGGAGTTAACACTTACTTAAGAGAAACATTTGGTGTTATTGAACCAGATTCAGGAAAACAAATATTAAACACTACTGTGGGTGGCGACAAATATAATACCCATAGAGGTGGACATCGATTTAAAGTTATTGTTGATAGAACCGAGAAAAAGCTTTGGCTTTCAATTGTAAATAACAATGGAATAGTTTTAGAAGGTAAAAACGAAGGTAAAAACACAAACTGGGATTTTTCAGTATTATCAAATGCTTTAGATAAAAAATTAGGAAAAATCGCAATTACTTATGGCGATGAAAAAGATGAAAACGGAATGCACTTTGTTCGATACACAAGAATGTTGATTATTCAAGGTCTATCTTTAGAAAAAATGTTAAAAGCTATAGAGTGTGGTGATTTAATGATCGATATAAGAATAGGAGTATATGCATCTGGTAAAAACAAAGGAAAAACACACGACCATGGCACTGCTTTTAGAATTAAATTAGAATCTTTGTTGAAATATGTTGATCTTTTGGAGATAGATTAA", "aa": "MTDNYEIELINFFKDIKSREWIETTRHGDQCLGNAFEDLLGVKENNKNEADFHGIELKTHRSITSSMVSLFSKSPSHPKGVNTYLRETFGVIEPDSGKQILNTTVGGDKYNTHRGGHRFKVIVDRTEKKLWLSIVNNNGIVLEGKNEGKNTNWDFSVLSNALDKKLGKIAITYGDEKDENGMHFVRYTRMLIIQGLSLEKMLKAIECGDLMIDIRIGVYASGKNKGKTHDHGTAFRIKLESLLKYVDLLEID", "aa_hexdigest": "bb583afa5c372dab1ff77bbdbd4cf91b", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158E1D4B", "ncbi_nrp_id": "WP_175949866.1", "uniref100_id": "UniRef100_UPI00158E1D4B", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E1D4B", "RefSeq:WP_175949866.1", "UniRef:UniRef100_UPI00158E1D4B" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E1D4B", "uniref90_id": "UniRef90_UPI00159302B7" }, "psc": { "uniref90_id": "UniRef90_UPI00159302B7", "product": "MvaI/BcnI family restriction endonuclease", "uniref50_id": "UniRef50_UPI00159302B7" }, "pscc": { "uniref50_id": "UniRef50_UPI00159302B7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00159302B7" ], "product": "MvaI/BcnI family restriction endonuclease" }, "genes": [], "id": "GLJLFKCOHC_572", "locus": "GCA013368035_00556" }, { "type": "cds", "sequence": "contig_1", "start": 320051, "stop": 320203, "strand": "-", "frame": 1, "gene": null, "product": "MvaI/BcnI family restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D3EE", "UniRef:UniRef90_UPI002410D3EE" ], "nt": "ATGGCTGAATCAATAGGAACTGGAATTTATGGAGAATTCAGATATATAGAAGCATATGAGATGTCTGGTTTTGACTACTATTCTTTTGTTGAATTACTACTAGAAAAATGTCAAATCTATGTGGATTTAAGAATTGGACAATATCATGGTTGA", "aa": "MAESIGTGIYGEFRYIEAYEMSGFDYYSFVELLLEKCQIYVDLRIGQYHG", "aa_hexdigest": "e98e2b76d74f70960a8d88a718f60114", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 319973, "stop": 320236, "observations": { "mutation": [ 320053 ], "stop": [ 320053 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410D3EE", "query_cov": 1.0, "subject_cov": 0.5747126436781609, "identity": 1.0, "score": 105.0, "evalue": 7.67e-29, "gene_start": 1, "gene_end": 50, "reference_start": 12, "reference_end": 61, "reference_sequence": "MKKYRNKFVYAMAESIGTGIYGEFRYIEAYEMSGFDYYSFVELLLEKCQIYVDLRIGQYHGWIKDGQTHDQGTGFRIIEIDKPYLLK" }, "paralog": false, "identity": 0.9770114942528736, "score": 177.0, "evalue": 9.36e-60, "description": "Internal stop codon at 320053. Nonsense mutation around 320053.", "psc": { "uniref90_id": "UniRef90_UPI002410D3EE", "product": "MvaI/BcnI family restriction endonuclease", "uniref50_id": "UniRef50_UPI002410D3EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410D3EE", "UniRef:UniRef50_UPI002410D3EE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410D3EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410D3EE" ], "product": "MvaI/BcnI family restriction endonuclease" } }, "id": "GLJLFKCOHC_573", "locus": "GCA013368035_00557" }, { "type": "cds", "sequence": "contig_1", "start": 320684, "stop": 320863, "strand": "-", "frame": 1, "gene": null, "product": "helix-turn-helix transcriptional regulator", "db_xrefs": [ "RefSeq:WP_241863013.1", "SO:0001217", "UniParc:UPI001F52741D", "UniRef:UniRef100_UPI001F52741D", "UniRef:UniRef50_UPI001F52741D", "UniRef:UniRef90_UPI001F52741D" ], "nt": "ATGGTAAGTTATAAGAAATTATGGAAACTTCTTATTGATAAAAATATTAATAAGAAAAAATTAATTGAGAAAACCGGAATTAGCACTTCTTCTATGGCAAAATTAACAAAAGGTGCCAATGTCACAACTGATGTTTTATGCAAATCTGTAAACATCTTGAGTGTGATTTTGGCGATATAA", "aa": "MVSYKKLWKLLIDKNINKKKLIEKTGISTSSMAKLTKGANVTTDVLCKSVNILSVILAI", "aa_hexdigest": "b8a8d5604b9943b649629a233821b55e", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI001F52741D", "ncbi_nrp_id": "WP_241863013.1", "uniref100_id": "UniRef100_UPI001F52741D", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F52741D", "RefSeq:WP_241863013.1", "UniRef:UniRef100_UPI001F52741D" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F52741D", "uniref90_id": "UniRef90_UPI001F52741D" }, "psc": { "uniref90_id": "UniRef90_UPI001F52741D", "product": "helix-turn-helix transcriptional regulator", "uniref50_id": "UniRef50_UPI001F52741D" }, "pscc": { "uniref50_id": "UniRef50_UPI001F52741D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F52741D" ], "product": "helix-turn-helix transcriptional regulator" }, "genes": [], "id": "GLJLFKCOHC_574", "locus": "GCA013368035_00558" }, { "type": "cds", "sequence": "contig_1", "start": 320878, "stop": 321096, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_175949867.1", "SO:0001217", "UniParc:UPI0015899D8D", "UniRef:UniRef100_UPI0015899D8D", "UniRef:UniRef50_UPI0015899D8D", "UniRef:UniRef90_UPI0015899D8D" ], "nt": "ATGACAGAGACAAGTGACGAGAACAATGAAGACGATTTAAGGAAAGGGTTCTTCAAATATATATTTGAAAACTACGAAGATGATCAGATTATCATTATCGAAAACACAGATAATCATGAACTACCTGAAGTTTCTGGTGAAACAAACAAAGTTATAGAATTTACTAAGAATAGAACACGCGGTAGATATGGCTTCTTAAAAGATGTGTATCAAAACTAA", "aa": "MTETSDENNEDDLRKGFFKYIFENYEDDQIIIIENTDNHELPEVSGETNKVIEFTKNRTRGRYGFLKDVYQN", "aa_hexdigest": "9c372e73c35cef8b61028c7c567a0cdb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0015899D8D", "ncbi_nrp_id": "WP_175949867.1", "uniref100_id": "UniRef100_UPI0015899D8D", "db_xrefs": [ "SO:0001217", "UniParc:UPI0015899D8D", "RefSeq:WP_175949867.1", "UniRef:UniRef100_UPI0015899D8D" ] }, "ips": { "uniref100_id": "UniRef100_UPI0015899D8D", "uniref90_id": "UniRef90_UPI0015899D8D" }, "psc": { "uniref90_id": "UniRef90_UPI0015899D8D", "uniref50_id": "UniRef50_UPI0015899D8D" }, "pscc": { "uniref50_id": "UniRef50_UPI0015899D8D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0015899D8D" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8628.257499999996, "isoelectric_point": 4.457905006408691 }, "id": "GLJLFKCOHC_575", "locus": "GCA013368035_00559" }, { "type": "cds", "sequence": "contig_1", "start": 321096, "stop": 321470, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001593BF4E", "UniRef:UniRef90_UPI001593BF4E" ], "nt": "GTGTCGTATCAACAAGCGTTAGCTATGTATGGAGACATGGAAAAGGAATATGGAACCGACTTTACTGAATATGATAAGGTTGAAGCAAAAATTAACTTCAAGCCAAGGAACCTATTCGCTAGCGATTTTGCTGTTAAACTTGCAAATAATTACAGAGATATTTTAATAGCAATGAACTTCACACCTGTAGAAACCGTTAAATTTGATATAGCGGCTTTTGATATGATAGTAAATGATAATCCAAAACCTAATCGAAGCAAGGGCTATGCTGCAATACTCAATTCAATTATTGTACTAGCACTGAGAAAATATATGAACGATCACGTGCGATCAATCCGCACTTCTACTTTCTTGATTCCCCTCTTCACGGCTTAA", "aa": "MSYQQALAMYGDMEKEYGTDFTEYDKVEAKINFKPRNLFASDFAVKLANNYRDILIAMNFTPVETVKFDIAAFDMIVNDNPKPNRSKGYAAILNSIIVLALRKYMNDHVRSIRTSTFLIPLFTA", "aa_hexdigest": "bfb0c71faa2e10d8db052fb77f75a159", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001593BF4E", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.992, "score": 244.0, "evalue": 9.31e-82, "valid": true, "uniref50_id": "UniRef50_UPI001593BF4E" }, "pscc": { "uniref50_id": "UniRef50_UPI001593BF4E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001593BF4E" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14233.258199999975, "isoelectric_point": 7.900423240661622 }, "id": "GLJLFKCOHC_576", "locus": "GCA013368035_00560" }, { "type": "cds", "sequence": "contig_1", "start": 321533, "stop": 321661, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCGCACAATTAATGACTTAAACGGATTAAATGAATCTAAGAAAGATCTTAAAGAAGAGAAAGCAGAACTAGAAGCAACCATTAGTGAGCTTGAAAATAAGAATAATAGGCTTGAACAGAACTAA", "aa": "MNRTINDLNGLNESKKDLKEEKAELEATISELENKNNRLEQN", "aa_hexdigest": "a1e1fb43ee9ad71f7a6c62716e6dd35e", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4887.3113, "isoelectric_point": 4.761823081970214 }, "id": "GLJLFKCOHC_577", "locus": "GCA013368035_00561" }, { "type": "cds", "sequence": "contig_1", "start": 322264, "stop": 322647, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTAAAGAAATTAATTTCTTAAGAATGGGATTTGCAATGATTTTTGGTACAGAAGATATGTATAGGAAAACTATAAAAGGTATGTATCGAGACGCATTTAGTTGCGAAGCTGAAATTGTAGAAATTGATTACAAAAGTTCATTGCCAATTGGTGAATGTGGAATTGATGCGTTTGGAAATAAGCCTTATTTTGAAGACATTGGAAATATTAAAAGAATAATTTGTGCAATCGGCAAATTAGACACGATGTTTGATGCGATGATACCTTATGATAAAGATTTAAAAATATTAGGTGGATCTTCAGACCATTTAATAATTAATGCTAACGATTCTAAAATTAATTATAAATGTGGGGACATACTTAAATTTGATTTAGATTGA", "aa": "MPKEINFLRMGFAMIFGTEDMYRKTIKGMYRDAFSCEAEIVEIDYKSSLPIGECGIDAFGNKPYFEDIGNIKRIICAIGKLDTMFDAMIPYDKDLKILGGSSDHLIINANDSKINYKCGDILKFDLD", "aa_hexdigest": "38f888a92d80150d9ecec61b56269e7a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14385.549899999978, "isoelectric_point": 4.871636009216308 }, "id": "GLJLFKCOHC_578", "locus": "GCA013368035_00562" }, { "type": "cds", "sequence": "contig_1", "start": 322657, "stop": 323277, "strand": "-", "frame": 2, "gene": null, "product": "Alanine racemase", "db_xrefs": [ "EC:5.1.1.12", "KEGG:K21898", "SO:0001217", "UniRef:UniRef50_A0A077L6Y5", "UniRef:UniRef90_A0A448ZWG6" ], "nt": "ATGTCATATCCTAAAATAATTATTGATGAGAAAAAATTTAAAAATAATATAAAAGTTGCTATTGAAATGTGTAAAAAACACAATATAGAAGTTCTTGCCGTTACTAAAGGTTTTTGCGGAAATCAAAGAATGGCTGAACTATATTATGAAGCAGGAATTAGGTATTTTGGTGATTCTAGACTTGAAAACTTTACTATCTTTAATAAATTTAAAGATGTTAAAAAACAATTATTAAGAATACCAATGATTTCAGAAATTTCTGAAGTTGTAAAAATTTGTGATTCAAGTTTAAATGGGGATATTCAAGTAATAGAAAAAATTAGTGAATATTGTCTTAAAAATAATTTAAAACCACATGAAATAGTTTTAATGATAGATTTAGGTGATAGAAGAGAAGGTGTTTTACCTGAAGATGCTTTGAGCACTGCTAAAAAAATACATGAACTAAAAGGTGTTAAATTAATTGGGTTAGGCTGTAACTTCGGTTGCTATGGCGCTAGAGTTCCCTCAGATGAATCAATGAAATTATTTGTAAATATTCAACACAAAATTGAAGATGAACTAAAAATTAAATTAACTCATATGTCTGGCGGAAATTCACTAAGCTTGCACCTAGTTTGA", "aa": "MSYPKIIIDEKKFKNNIKVAIEMCKKHNIEVLAVTKGFCGNQRMAELYYEAGIRYFGDSRLENFTIFNKFKDVKKQLLRIPMISEISEVVKICDSSLNGDIQVIEKISEYCLKNNLKPHEIVLMIDLGDRREGVLPEDALSTAKKIHELKGVKLIGLGCNFGCYGARVPSDESMKLFVNIQHKIEDELKIKLTHMSGGNSLSLHLV", "aa_hexdigest": "1cc30a780769c099790ba566636809fe", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 322354, "stop": 323277, "observations": { "mutation": [ 322659 ], "stop": [ 322659 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A448ZWG6", "query_cov": 1.0, "subject_cov": 0.5786516853932584, "identity": 0.995, "score": 409.0, "evalue": 3.53e-142, "gene_start": 1, "gene_end": 206, "reference_start": 1, "reference_end": 206, "reference_sequence": "MSYPKIIIDEKKFKNNIKVAIEMCKKHNIEVLAVTKGFCGNQRMAELYYEAGIRYFGDSRLENFTIFNKFKDVKKQLLRIPMISEIPEVVKICDSSLNGDIQVIEKISEYCLKNNLKPHEIVLMIDLGDRREGVLPEDALSTAKKIHELKGVKLIGLGCNFGCYGARVPSDESMKLFVNIQHKIEDELKIKLTHMSGGNSLSLHLVWENRMPKEINFLRMGFAMIFGTEDMYRKTIKGMYRDAFSCEAEIVEIDYKSSLPIGECGIDAFGNKPYFEDIGNIKRIICAIGKLDTMFDAMIPYDKDLKILGGSSDHLIINANDSKINYKCGDILKFDLDWGSLLYLFNSKYVKKEFKK" }, "paralog": false, "identity": 0.993485342019544, "score": 614.0, "evalue": 2.01e-225, "description": "Internal stop codon at 322659. Nonsense mutation around 322659.", "psc": { "uniref90_id": "UniRef90_A0A448ZWG6", "product": "Alanine racemase", "ec_ids": [ "5.1.1.12" ], "uniref50_id": "UniRef50_A0A077L6Y5", "kegg_orthology_id": "K21898", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A448ZWG6", "EC:5.1.1.12", "UniRef:UniRef50_A0A077L6Y5", "KEGG:K21898" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L6Y5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L6Y5" ], "product": "Alanine racemase-like protein" } }, "id": "GLJLFKCOHC_579", "locus": "GCA013368035_00563" }, { "type": "cds", "sequence": "contig_1", "start": 323634, "stop": 323915, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAATACACATTGTTCATTTGAAGAAAAAATGGAAACAATTAAAAGAGTTTTATTAAGCAAAGGAATTAATCTAGTAACGATGCCTTTTATTGAAAATAGTTTGATAAGGGCTATTGCTTTGAAAAAGGGAGCAAAAAGGTATATTTTCCTTTCTGATATGTTTAATTCTGAAGCATATATATTTTATTCATTGCTACATGAAATAGTTCATTGCTTTTTTTCAAATTATACTGAAAACAAAATTGATAAAGTTGTTATCAAAGAATATTTTGCATGA", "aa": "MSNTHCSFEEKMETIKRVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFA", "aa_hexdigest": "58535b77e1c05b428edfe242ee4a00ae", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10923.70269999999, "isoelectric_point": 8.565285682678223 }, "id": "GLJLFKCOHC_580", "locus": "GCA013368035_00564" }, { "type": "cds", "sequence": "contig_1", "start": 324051, "stop": 324533, "strand": "-", "frame": 3, "gene": null, "product": "HTH cro/C1-type domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844", "UniRef:UniRef90_D1J844" ], "nt": "ATGATTGAAGAGCACATCTGTGATATGAAGCACGTTAAAATTCATGGCAATGATTTTACAGATGAAATAAAATACTATTTAAAAATGTTTTCAATGACTCAAAAAGAATTATCTATAAGACTTGGATTATCAATAAAACATATCAATTCAATTATGAATAATGAAGTTAATAACGTTAGTGCAACTATTATTGAGGCGCTTGAATATGCATTTCACTTAGAAATTGGCACATTAACAGAAGTTTATCATATTTATAATAACCTAAAAACGTTAAAGAAAAATAAGAATATTGAAGATGATTTAAAGAAGCATGGTATTGATTTTTTAATAAATCATCCAGAGCTTGCATTGGCAGCTAACATTAGCATTCATGAAAATAGCCCACTACATGTTAAATTAATAATGCTAAAAAGATTTTTTGGTGTTGCAGACTTGGCTAACTATGATAAATATTTAAAAGATAATGTTTCAGCAGAAGAATGA", "aa": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEE", "aa_hexdigest": "e07654f3e13b12c233b1754b5b25cdd6", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 323748, "stop": 324533, "observations": { "mutation": [ 324023, 324053 ], "stop": [ 324023, 324053 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J844", "query_cov": 1.0, "subject_cov": 0.4456824512534819, "identity": 1.0, "score": 322.0, "evalue": 1.35e-108, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MIEEHICDMKHVKIHGNDFTDEIKYYLKMFSMTQKELSIRLGLSIKHINSIMNNEVNNVSATIIEALEYAFHLEIGTLTEVYHIYNNLKTLKKNKNIEDDLKKHGIDFLINHPELALAANISIHENSPLHVKLIMLKRFFGVADLANYDKYLKDNVSAEEWTYSNPNTKVWIRFCELLSIDKNIDENVGIFRKSSFNSLYHKILTIMSNTHCSFEEKMETIKKVLLSKGINLVTMPFIENSLIRAIALKKGAKRYIFLSDMFNSEAYIFYSLLHEIVHCFFSNYTENKIDKVVIKEYFAWEKNNPTTYKAIYDAINSYQQCEAIQKANKNIDTSYIWSTLKDKYPYVAFDPLSDQKSKN" }, "paralog": false, "identity": 0.9885057471264368, "score": 511.0, "evalue": 1.63e-185, "description": "Internal stop codon at 324023, 324053. Nonsense mutation around 324023, 324053.", "psc": { "uniref90_id": "UniRef90_D1J844", "product": "HTH cro/C1-type domain-containing protein", "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J844", "UniRef:UniRef50_D1J844" ] }, "pscc": { "uniref50_id": "UniRef50_D1J844", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J844" ], "product": "HTH cro/C1-type domain-containing protein" } }, "id": "GLJLFKCOHC_581", "locus": "GCA013368035_00565" }, { "type": "cds", "sequence": "contig_1", "start": 324707, "stop": 325102, "strand": "-", "frame": 1, "gene": "cdd", "product": "cytidine deaminase", "db_xrefs": [ "COG:COG0295", "COG:F", "EC:3.5.4.5", "GO:0004126", "GO:0008270", "GO:0009972", "KEGG:K01489", "RefSeq:WP_036438513.1", "SO:0001217", "UniParc:UPI0005144531", "UniRef:UniRef100_A0A454C9K2", "UniRef:UniRef50_D5E4U5", "UniRef:UniRef90_A0A454C9K2" ], "nt": "ATGACAATAGAAGAATTAAAAAATAAATTAAAATATTCATTTAGTCCTTATTCACATGTTCAAGTTGCATGTTGTGCAATTGAAGAAAATGGTAAAGAATATTGGGGAGTCAATACTGAAAACGCTGCATTTCCATCTGGATTATGTGCTGAAAGAAGTGCATTATTTGGTTCTGTAGCATATGGCGCTAGAGTTGGTAATTTTAAAGAAATACATATAATTAGCAATTTATCTAAATTGTTATATCCTTGCGGTGCTTGTGCCCAAGTTATTAGCCAATTTTTAAAACCAGATGGCGATGTATATTTATACAGTTTTGATTTAAAGCAACAAAAGCATTATAAAATGAAAGATATTCTACCTTTTGCAGTTGCACCAAAAGATATAGTGGATTAG", "aa": "MTIEELKNKLKYSFSPYSHVQVACCAIEENGKEYWGVNTENAAFPSGLCAERSALFGSVAYGARVGNFKEIHIISNLSKLLYPCGACAQVISQFLKPDGDVYLYSFDLKQQKHYKMKDILPFAVAPKDIVD", "aa_hexdigest": "675e8b3d310036b1f9d0a200199bd6cb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0005144531", "ncbi_nrp_id": "WP_036438513.1", "uniref100_id": "UniRef100_A0A454C9K2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005144531", "RefSeq:WP_036438513.1", "UniRef:UniRef100_A0A454C9K2" ] }, "ips": { "uniref100_id": "UniRef100_A0A454C9K2", "uniref90_id": "UniRef90_A0A454C9K2" }, "psc": { "uniref90_id": "UniRef90_A0A454C9K2", "gene": "Cdd", "product": "cytidine deaminase", "ec_ids": [ "3.5.4.5" ], "uniref50_id": "UniRef50_D5E4U5", "cog_id": "COG0295", "cog_category": "F", "kegg_orthology_id": "K01489", "go_ids": [ "GO:0004126", "GO:0008270", "GO:0009972" ] }, "pscc": { "uniref50_id": "UniRef50_D5E4U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E4U5" ], "product": "Cytidine deaminase" }, "genes": [ "cdd" ], "id": "GLJLFKCOHC_582", "locus": "GCA013368035_00566" }, { "type": "cds", "sequence": "contig_1", "start": 325254, "stop": 325511, "strand": "+", "frame": 3, "gene": "rpsP", "product": "30S ribosomal protein S16", "db_xrefs": [ "COG:COG0228", "COG:J", "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627", "RefSeq:WP_012855534.1", "SO:0001217", "UniParc:UPI0001BC2233", "UniRef:UniRef100_D1J846", "UniRef:UniRef50_B3PMA1", "UniRef:UniRef90_D1J846" ], "nt": "ATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCCGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGGACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAA", "aa": "MVKLRLKRTGKKFYATYKIVAADARSPRDGKFIEELGYYDPNSKKLVLNKELIIKHLDNGAKPSDTVRNLLKQDNFYADYVASKK", "aa_hexdigest": "b48a36ad0c503e23671ac76fd94afdb2", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2233", "ncbi_nrp_id": "WP_012855534.1", "uniref100_id": "UniRef100_D1J846", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2233", "RefSeq:WP_012855534.1", "UniRef:UniRef100_D1J846" ] }, "ips": { "uniref100_id": "UniRef100_D1J846", "uniref90_id": "UniRef90_D1J846" }, "psc": { "uniref90_id": "UniRef90_D1J846", "gene": "rpsP", "product": "30S ribosomal protein S16", "uniref50_id": "UniRef50_B3PMA1", "cog_id": "COG0228", "cog_category": "J", "go_ids": [ "GO:0000314", "GO:0003735", "GO:0006412", "GO:0022627" ] }, "pscc": { "uniref50_id": "UniRef50_B3PMA1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PMA1" ], "product": "Small ribosomal subunit protein bS16" }, "genes": [ "rpsP" ], "id": "GLJLFKCOHC_583", "locus": "GCA013368035_00567" }, { "type": "cds", "sequence": "contig_1", "start": 325524, "stop": 326126, "strand": "+", "frame": 3, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "db_xrefs": [ "COG:COG0336", "COG:J", "EC:2.1.1.228", "GO:0008033", "GO:0052906", "KEGG:K00554", "SO:0001217", "UniRef:UniRef50_A9NHF7", "UniRef:UniRef90_D1J847" ], "nt": "ATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAGATTATTCATTAGATAAACATCATAAAGTTGATGATAGCCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATCGAACCAATAGATTTAGCACTACAACCTTTAAAAAATAGTCACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCAAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTAGATGAAGAAATATCAATTGGAGATTTTATCTTAACAGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATATACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGA", "aa": "MKINILTLFPDYLNAFCNQSMIAKALELGHIEINIINFRDYSLDKHHKVDDSPYGGGAGMLLQIEPIDLALQPLKNSHKILLSPQGKVFSQKKAHELAKLNEITLICGHYEGFDERITKLVDEEISIGDFILTGGEIAAMAISEAVARLIPGVLRKNSYENESFEGNGLLDYPQYTRPAVYKGMKVPKILLSGNHLEIEK", "aa_hexdigest": "5267e1237e2a3031a4db6e05b9bc9830", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J847", "query_cov": 1.0, "subject_cov": 0.9009009009009009, "identity": 0.995, "score": 400.0, "evalue": 7.73e-141, "valid": true, "gene": "trmD", "product": "tRNA (guanosine(37)-N1)-methyltransferase TrmD", "ec_ids": [ "2.1.1.228" ], "uniref50_id": "UniRef50_A9NHF7", "cog_id": "COG0336", "cog_category": "J", "kegg_orthology_id": "K00554", "go_ids": [ "GO:0008033", "GO:0052906" ] }, "pscc": { "uniref50_id": "UniRef50_A9NHF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A9NHF7" ], "product": "tRNA (guanine-N(1)-)-methyltransferase" }, "genes": [ "trmD" ], "id": "GLJLFKCOHC_584", "locus": "GCA013368035_00568" }, { "type": "cds", "sequence": "contig_1", "start": 326194, "stop": 326550, "strand": "+", "frame": 1, "gene": "rplS", "product": "50S ribosomal protein L19", "db_xrefs": [ "COG:COG0335", "COG:J", "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02884", "RefSeq:WP_012855536.1", "SO:0001217", "UniParc:UPI0001BC2235", "UniRef:UniRef100_D1J848", "UniRef:UniRef50_Q044E2", "UniRef:UniRef90_D1J848" ], "nt": "ATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGTACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGAGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGCAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCTAGATTAAAAGAAATTAAAAACTCTAAATAA", "aa": "MRTKLLELVEKDQIREDLPQLREGYNVRVHVRIKEGDKERIQVFEGLIIAEYGTGINKSITVRKESYGVGVERIFKINSPLIAQIEVIRRNKVRRAKLYYMRDLKGKSARLKEIKNSK", "aa_hexdigest": "9c890839eeb918280e6bf122bb6ab676", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0001BC2235", "ncbi_nrp_id": "WP_012855536.1", "uniref100_id": "UniRef100_D1J848", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2235", "RefSeq:WP_012855536.1", "UniRef:UniRef100_D1J848" ] }, "ips": { "uniref100_id": "UniRef100_D1J848", "uniref90_id": "UniRef90_D1J848" }, "psc": { "uniref90_id": "UniRef90_D1J848", "gene": "rplS", "product": "50S ribosomal protein L19", "uniref50_id": "UniRef50_Q044E2", "cog_id": "COG0335", "cog_category": "J", "kegg_orthology_id": "K02884", "go_ids": [ "GO:0000311", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q044E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q044E2" ], "product": "Large ribosomal subunit protein bL19" }, "genes": [ "rplS" ], "id": "GLJLFKCOHC_585", "locus": "GCA013368035_00569" }, { "type": "cds", "sequence": "contig_1", "start": 326761, "stop": 327018, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACACACAAAAATTTTATTAGCATTAGGAAGCATTGCAACCTCATTTGCCGCAATACCATTAGTTGCTGCAGGTTGTGTAAAAACAAAAAAACCAGAAGTTAAACCAGAAGAACCTAAAAAACCAGAAGGTGAAAAACCAGGAACAACACCTGAAGATCCAAAGAAACCAGAAGGTGAAAAGCCAGGAACAACTCCAGAAGTTAAACCAGAGGATAAAAAATCAGAAGAACAAAAAGAAATAGATAATTGA", "aa": "MKKHTKILLALGSIATSFAAIPLVAAGCVKTKKPEVKPEEPKKPEGEKPGTTPEDPKKPEGEKPGTTPEVKPEDKKSEEQKEIDN", "aa_hexdigest": "468a66852edb3128e7003145bc788a4b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9186.473999999998, "isoelectric_point": 7.788699531555175 }, "id": "GLJLFKCOHC_586", "locus": "GCA013368035_00570" }, { "type": "cds", "sequence": "contig_1", "start": 327291, "stop": 327506, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTTCTAGTTTAACATCATCGGCAGTTAATTTGTTAATTTCTTTGTTTTTGTCTTTGATATCAAATTTAAGATCTTTTATAATTTTATCTAATTGTGTTTCTGATAGATCTATTTCATTGCTTACAATTTTAGTTCCAATAATAGGATCTTTTCTTGCAACAAATTTAGCTCCTTTAAATTTTAATGTTATCTTATTGTCTTTTAATTCATAG", "aa": "MPSSLTSSAVNLLISLFLSLISNLRSFIILSNCVSDRSISLLTILVPIIGSFLATNLAPLNFNVILLSFNS", "aa_hexdigest": "16b4dd445e71ad257f99816ca7f01344", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7610.965999999997, "isoelectric_point": 7.999833488464356 }, "id": "GLJLFKCOHC_587", "locus": "GCA013368035_00571" }, { "type": "cds", "sequence": "contig_1", "start": 327805, "stop": 327912, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTTATCTAAAAGGAAATCATAATATTATTATTGAATTTAATGGCCAAAAACCAAATGAAACCTTAAGTCAAGTTGTATTTGAAGGAAAATCCGGAGCATTTTGA", "aa": "MFYLKGNHNIIIEFNGQKPNETLSQVVFEGKSGAF", "aa_hexdigest": "714b5cc7067f23650074264dc18cf3d0", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3958.4535000000005, "isoelectric_point": 6.520194053649903 }, "id": "GLJLFKCOHC_588", "locus": "GCA013368035_00572" }, { "type": "cds", "sequence": "contig_1", "start": 328184, "stop": 328594, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22421.1" ], "nt": "ATGACTCAACACAATGAAAATAAAACCAAAAGAATTGCCCAAAAAAAATTAGCCTTTGAATTGGTTAAAGACATTCATGGAGTTGATATTGCAAACAAATGTATTAGAATAAGTGAGATTTTATTCAATAAAAGTAACGAAGAATTAAACATAGAAGATATTTCGATGCTTTTAGGCTATTTGTCATGTATTGAAGTTAAAGAAGGCGAAAAATTTATTGAAATTTTAAAAAATAATCAAATTGTGTCTTCAAATAGAGAAGCAAGAGAATTTATTTCAAAAAATACTTTTTTAATTGATAACAATATAATAAACGATGAAAATTATTCAATTGCATTTAATAAATTTAATGGTCAATATGCGCTATTAAAAAAAGGCAAAAAAGACTTTTACATACTTAAAAGAATTTAA", "aa": "MTQHNENKTKRIAQKKLAFELVKDIHGVDIANKCIRISEILFNKSNEELNIEDISMLLGYLSCIEVKEGEKFIEILKNNQIVSSNREAREFISKNTFLIDNNIINDENYSIAFNKFNGQYALLKKGKKDFYILKRI", "aa_hexdigest": "6b3af685b059b0fb9ef4c4002f72ada2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22421.1", "name": "SYY_C-terminal", "length": 71, "aa_cov": 0.5073529411764706, "hmm_cov": 1.0, "evalue": 3.796508197444604e-13, "score": 44.611263275146484, "start": 64, "stop": 132 } ], "seq_stats": { "molecular_weight": 15874.140899999984, "isoelectric_point": 8.798983573913574 }, "id": "GLJLFKCOHC_589", "locus": "GCA013368035_00573" }, { "type": "cds", "sequence": "contig_1", "start": 328631, "stop": 328789, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTCAATTTATTAGTTGACGAAAATGGCAAAAAATTTGGTAAAAGCACTGGCGGAGGTTCGTTATGGCTTGATCCAAATATGACTAGTCCATATTCTATATATCAATTCCTATTGAATCAAAGTGATGTAAAAATCGAAGAATATTTAAAATGA", "aa": "MTFNLLVDENGKKFGKSTGGGSLWLDPNMTSPYSIYQFLLNQSDVKIEEYLK", "aa_hexdigest": "4f8361fe08f003b759ad8e2e669c4a83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5918.6582, "isoelectric_point": 4.992987251281736 }, "id": "GLJLFKCOHC_590", "locus": "GCA013368035_00574" }, { "type": "cds", "sequence": "contig_1", "start": 329054, "stop": 329419, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ], "nt": "ATGCAAAAAAATATATTAGATGATTTGAAATCAAGAGGTGTTTTAAATAATATATCTAGTGAAGAAAAATTTAATAATATATCACTAGGAACAGGTGTTTATACAGGTTTTGATCCAACTGCTAAAAGTTTACACCTTGGAAATTATCTACAAATATTAAGTTTGTTACGTTTTAAAAAAGCAGGATATAAACCAATTGCAATATTGGGTGGAATAACTGGAATGATTGGTGATCCTAGTTTTAGAAATTCTGAAAGAAAATTTCTAGATCAAGAAACATTACTACAAAATAAAAATGCAATTAAAAGTCAATTACAAAAATTTGGTTTAGAAGTAATTGATAATTTAGATTTTTATAAGCATTGA", "aa": "MQKNILDDLKSRGVLNNISSEEKFNNISLGTGVYTGFDPTAKSLHLGNYLQILSLLRFKKAGYKPIAILGGITGMIGDPSFRNSERKFLDQETLLQNKNAIKSQLQKFGLEVIDNLDFYKH", "aa_hexdigest": "7a7efad5c891d11cfbea0d8a3af82c33", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI002FEF080B", "query_cov": 0.9752066115702479, "subject_cov": 0.9752066115702479, "identity": 0.6609999999999999, "score": 158.0, "evalue": 7.38e-48, "valid": false, "product": "tRNA synthetases class I (W and Y)", "ec_ids": [ "6.1.1.1" ], "uniref50_id": "UniRef50_UPI002FEF080B", "kegg_orthology_id": "K01866" }, "pscc": { "uniref50_id": "UniRef50_UPI002FEF080B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FEF080B" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13606.473599999983, "isoelectric_point": 9.362501716613771 }, "id": "GLJLFKCOHC_591", "locus": "GCA013368035_00575" }, { "type": "cds", "sequence": "contig_1", "start": 329753, "stop": 330424, "strand": "+", "frame": 2, "gene": "degV", "product": "DegV family protein", "db_xrefs": [ "COG:COG1307", "COG:I", "SO:0001217", "UniRef:UniRef50_A0A077L663", "UniRef:UniRef90_D1J851" ], "nt": "ATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAGTCTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTCGATGAGGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCGGAGTTTAACGATGCCTTGCTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAACGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAAACAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCGTTTAAACAAATAACCAATAATTTTCCAAGGATTTATACAATTCAATTGAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCCGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATACTTAAAAAAACAATAA", "aa": "MQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ", "aa_hexdigest": "f2407cbe3a1718095f5c23dcbc34070f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "degV" ], "truncated": "5-prime", "pseudogene": { "start": 329543, "stop": 330424, "observations": { "mutation": [ 329609, 329696 ], "stop": [ 329609, 329696 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J851", "query_cov": 1.0, "subject_cov": 0.7610921501706485, "identity": 1.0, "score": 432.0, "evalue": 3.96e-152, "gene_start": 1, "gene_end": 223, "reference_start": 71, "reference_end": 293, "reference_sequence": "MKIKIITDSSAGMSQEQANSFGWDLIPIQIEVGNDIYKSGIDFDTDKFEELWRNDKKIDAKTFATPPGIAMQVIEKYFNDYDKILVYPISNALSSQCASLTAQFASNPKVHVVQSNKISYLIVRDLLMFEEYIKEGKSFDEAIEHFKNNNERLLLIPEFNDALLKGGRLSKAAAAIAKLLKIVPIIKFDNGILEKDGIGRIFKKTLEKNLKELYENTNKDNENNYLLVVNANNDKIDELIQSFKQITNNFPRIYTIQLSCDIVVHAGIGAICLTFAEVSPKIKDKFFLYLKKQ" }, "paralog": false, "identity": 0.9897610921501706, "score": 567.0, "evalue": 9.94e-208, "description": "Internal stop codon at 329609, 329696. Nonsense mutation around 329609, 329696.", "psc": { "uniref90_id": "UniRef90_D1J851", "gene": "DegV", "product": "DegV family protein", "uniref50_id": "UniRef50_A0A077L663", "cog_id": "COG1307", "cog_category": "I", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J851", "UniRef:UniRef50_A0A077L663", "COG:COG1307", "COG:I" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L663", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L663" ], "product": "DegV family protein" } }, "id": "GLJLFKCOHC_592", "locus": "GCA013368035_00576" }, { "type": "cds", "sequence": "contig_1", "start": 330465, "stop": 330728, "strand": "-", "frame": 3, "gene": "rpmA", "product": "50S ribosomal protein L27", "db_xrefs": [ "COG:COG0211", "COG:J", "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625", "KEGG:K02899", "RefSeq:WP_012855540.1", "SO:0001217", "UniParc:UPI0001BC2239", "UniRef:UniRef100_D1J852", "UniRef:UniRef50_O21275", "UniRef:UniRef90_D1J852" ], "nt": "ATGGCACATACGAAATCAGGTGGAACAACCGCCAATAGCCGTGATTCAGCGGGAAGAAGATTAGGCGTAAAAGCAACCGACGGCCAATTTATTTTAGCAGGAAGCATTATTTATAGACAAAGAGGAACAAAAATTTTCCCAGGCCAAAATGTTGGTAGAGGAAAAGATGACACCTTATTTGCTTTAATCGATGGTATTGTAAAATTCGAAAGCAGAATTAACAGAAAATTTGCATCAGTTTACCCAATTGAAAGCAAGAAATAA", "aa": "MAHTKSGGTTANSRDSAGRRLGVKATDGQFILAGSIIYRQRGTKIFPGQNVGRGKDDTLFALIDGIVKFESRINRKFASVYPIESKK", "aa_hexdigest": "8bf9087f30d2afc010cb018fb10ca22d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2239", "ncbi_nrp_id": "WP_012855540.1", "uniref100_id": "UniRef100_D1J852", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2239", "RefSeq:WP_012855540.1", "UniRef:UniRef100_D1J852" ] }, "ips": { "uniref100_id": "UniRef100_D1J852", "uniref90_id": "UniRef90_D1J852" }, "psc": { "uniref90_id": "UniRef90_D1J852", "gene": "rpmA", "product": "50S ribosomal protein L27", "uniref50_id": "UniRef50_O21275", "cog_id": "COG0211", "cog_category": "J", "kegg_orthology_id": "K02899", "go_ids": [ "GO:0000315", "GO:0003735", "GO:0006412", "GO:0022625" ] }, "genes": [ "rpmA" ], "id": "GLJLFKCOHC_593", "locus": "GCA013368035_00577" }, { "type": "cds", "sequence": "contig_1", "start": 330731, "stop": 331033, "strand": "-", "frame": 1, "gene": "rplU", "product": "50S ribosomal protein L21", "db_xrefs": [ "COG:COG0261", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855541.1", "SO:0001217", "UniParc:UPI0001BC223A", "UniRef:UniRef100_D1J853", "UniRef:UniRef50_Q6F123", "UniRef:UniRef90_D1J853" ], "nt": "ATGTTTGCTATTATTGAAACCGGCGGAAAACAATTACTTGTTAAAAGCGGTGATGTAATTTACATCGAAAAAATTGATGGCAATGAAGGTGACAAAGTTTCATTTGATAAAGTTTTAGTTATTGATGAACAAGTTGGCTCACCTTATCTACCAAAAGCTTCTGTTTTAGGAACCATCGAAAAACAAGGAAAAGCAAAAAAAATAGTTGTTTATAGACATAATGCTAAATCTACTCATAAACGTAAATTAGGACATCGTCAACCATTTACCAGAGTTAAAATTACAGAAATTAAGGAAAAATAG", "aa": "MFAIIETGGKQLLVKSGDVIYIEKIDGNEGDKVSFDKVLVIDEQVGSPYLPKASVLGTIEKQGKAKKIVVYRHNAKSTHKRKLGHRQPFTRVKITEIKEK", "aa_hexdigest": "2e1b8b13d765a237bc4b0b6090d9d3bf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223A", "ncbi_nrp_id": "WP_012855541.1", "uniref100_id": "UniRef100_D1J853", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223A", "RefSeq:WP_012855541.1", "UniRef:UniRef100_D1J853" ] }, "ips": { "uniref100_id": "UniRef100_D1J853", "uniref90_id": "UniRef90_D1J853" }, "psc": { "uniref90_id": "UniRef90_D1J853", "gene": "rplU", "product": "50S ribosomal protein L21", "uniref50_id": "UniRef50_Q6F123", "cog_id": "COG0261", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6F123", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6F123" ], "product": "Large ribosomal subunit protein bL21" }, "genes": [ "rplU" ], "id": "GLJLFKCOHC_594", "locus": "GCA013368035_00578" }, { "type": "cds", "sequence": "contig_1", "start": 332404, "stop": 332679, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAATTACATTAAAATGGTTAACAATTTTAGGAGCAACAACGCTTACTGCCTTGACTTTTTGTGCAATTTCTTGTAGTAATGAGGAAAAAGAAATTGAAAAAGCAGAGAACATTATTGGCGATAAAGAACTAACATTTAAAATAGATGATTTAAAATATGAAAATTCTGATAATTCTATATTGCAAAGTGCTAGAAGTATTGCAGGACCATGGCGCAAGGACTTTCTTAAGGTTGTTATAACGTCAAAATCAAATATTGAAGAATATTGA", "aa": "MKKITLKWLTILGATTLTALTFCAISCSNEEKEIEKAENIIGDKELTFKIDDLKYENSDNSILQSARSIAGPWRKDFLKVVITSKSNIEEY", "aa_hexdigest": "9508797b6e01d910ee191132e5b08056", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10315.75669999999, "isoelectric_point": 5.447187614440917 }, "id": "GLJLFKCOHC_595", "locus": "GCA013368035_00579" }, { "type": "cds", "sequence": "contig_1", "start": 332785, "stop": 333108, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U0", "UniRef:UniRef90_A0A454C9U0" ], "nt": "ATGGATCTAAAATCTTCTTACCAAAAAGCAAACAAAGCATATTTAATAGAAAAACCAAAAAAAGTATATTCAAATCTAAAAAATAAACAAATATTAATAAATAACTACACTATATTTTTTGATGATATAAATAATGATAAATCTCCAAGAGTTTCAATGGCGATAAAAGAATTAAAATTTAATTTTGATCAAGACCGAAAAACCATAGAAATTAAACTTGGTTTAAGATATGAATTATCACAAAAACATAATAGGAAGTCTAATATATTTTTTCTTTCTCCTAAGGCAACCTTTAAATACATTTTAAGCGAAGAAATCAAATAA", "aa": "MDLKSSYQKANKAYLIEKPKKVYSNLKNKQILINNYTIFFDDINNDKSPRVSMAIKELKFNFDQDRKTIEIKLGLRYELSQKHNRKSNIFFLSPKATFKYILSEEIK", "aa_hexdigest": "3ae9af9080292910bf3cae1e53afa526", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 332485, "stop": 333108, "observations": { "mutation": [ 332677, 332689 ], "stop": [ 332677, 332689 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U0", "query_cov": 1.0, "subject_cov": 0.45726495726495725, "identity": 0.981, "score": 201.0, "evalue": 9.53e-64, "gene_start": 1, "gene_end": 107, "reference_start": 128, "reference_end": 234, "reference_sequence": "MKKITLKWLTILGATTLTALPFCAISCSNEEKEIEKAEKIIGDKELTFKIDDLKYENSDNSILQSARSIAGPWRKDFLKVVITSKSNIEEYWKDNWIVGKIDLKNKAILFLIPREVLLDADFIKEKNMDLKSSYQKANKAYLIEKPKKVYSNLKNKQIWINNYTIFFDDINNDKSPRVSMAIKELKFNFDQDQKTIEIKLGLRYELSQKHNRKSNIFFLSPKATFKYILSEEIK" }, "paralog": false, "identity": 0.9758454106280193, "score": 393.0, "evalue": 8.61e-142, "description": "Internal stop codon at 332677, 332689. Nonsense mutation around 332677, 332689.", "psc": { "uniref90_id": "UniRef90_A0A454C9U0", "product": "Lipoprotein", "uniref50_id": "UniRef50_A0A454C9U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U0", "UniRef:UniRef50_A0A454C9U0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U0" ], "product": "Lipoprotein" } }, "id": "GLJLFKCOHC_596", "locus": "GCA013368035_00580" }, { "type": "cds", "sequence": "contig_1", "start": 333662, "stop": 334291, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAATTCAAAGAATTTATCGTATTCAAGGTATTGCAATTAGCGATAAGTATATTGAAATTATAATTCGTCAAATGATGTCTAAATTAGTAATTTCTGAACCCGGAGATTCTAAATTCTTCGCTGGTGCTATTGTAGATATTTTTGACTATCAAGAAGAAAATGCTATTCTACTTTCAAAAGGTTTGAAGCCAGCATATGGTAAAGTTGTTATTAAAGGTGCAAAACAAGTTCCATTGTTATCTGATTCATTCCTTGCCGCTGCTTCTTATCAAGAAACAAGCAAGATATTAGTTCACGCTGCTATTTCATCAAGAATTGACAATTTATCTGGCTTAAAAGAAAATATTATTCTTGGTAAAAAGATTCCATCAGGAACAGCATTATTCCCATTTGAAGAACATTCAAAATATGATATAAAACCATCAATTAATTACTTTACAACAACTCCTATTGACGGCACAGAAATTTCTGAACCTATTGATATTGATTCAGTTGTTAATACAGCGCAAGAATATGACAATGATAAACAATCAGTTGAAGAAGATTATTCTCCAAACGACGAAGAACTTGTTGCTGAAGAAGAAACATTTGATGATGAAGAAAATCAAAACGATTTTGAATAA", "aa": "MKEIQRIYRIQGIAISDKYIEIIIRQMMSKLVISEPGDSKFFAGAIVDIFDYQEENAILLSKGLKPAYGKVVIKGAKQVPLLSDSFLAAASYQETSKILVHAAISSRIDNLSGLKENIILGKKIPSGTALFPFEEHSKYDIKPSINYFTTTPIDGTEISEPIDIDSVVNTAQEYDNDKQSVEEDYSPNDEELVAEEETFDDEENQNDFE", "aa_hexdigest": "fbd290a4b427df44851d560d6d23fcd8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23438.981199999995, "isoelectric_point": 4.424085807800293 }, "id": "GLJLFKCOHC_597", "locus": "GCA013368035_00581" }, { "type": "cds", "sequence": "contig_1", "start": 334559, "stop": 335320, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF05000.22", "PFAM:PF04998.22" ], "nt": "ATGATTAACTCAGGTGCTCGTGGTAACATTTCTAACTATGTTCAATTAGCAGGTATGCGTGGATTGATGGCTAACAATACTAAAACTACTAAGGCCGATGCTAAAAACGACCGTGTTGTTAGATCAACAGTTGAAGTTCCTGTTAAGTCTTCATTTATTGAAGGACTTACTGCATTTGAATTCTATTCATCAACTCACGGTGCTAGAAAAGGTTTAACCGATACTGCCTTGAACACTGCAAAATCTGGTTATTTAACAAGACGTTTAGTTGATGTTGCTCAAAATATTGTTGTTAGAGAAGAAAACTGTGGTACTGACTATGGTTATAAAGCAAGAAACATAGTTGATACAAAAACTAAACAAATTATTGTTCCTTTGAAAGAAAGAATTGTTGGAAGATTTGCAAACAAGCCCGTTGTTACAAGTGCGGGTATAGAAATTTGTTCTAGAAATGAATTAATTACTGAAAAAATTGCTCAACGAATAATAGCAAGTGGTATTGAAGAAGTCGAAATTAGATCAATCTTAGGCTGCAATACACGTAACGGTGTATGTAAGATGTGTTTTGGTAAAGACCTTGCAACAAATAGAATTGTTAATATCGGTGAAGCAGTAGGTATTATTGCAGCTCAATCTATTGGGGAACCAGGAACTCAATTAACTATGCGTACATTCCACACTGGTGGTGTTGCTGGGGTTGAAGATATTACTGGTGGTTTCACTAGATTGATCGAATTAATTGATGCCCACGAACAACCATGA", "aa": "MINSGARGNISNYVQLAGMRGLMANNTKTTKADAKNDRVVRSTVEVPVKSSFIEGLTAFEFYSSTHGARKGLTDTALNTAKSGYLTRRLVDVAQNIVVREENCGTDYGYKARNIVDTKTKQIIVPLKERIVGRFANKPVVTSAGIEICSRNELITEKIAQRIIASGIEEVEIRSILGCNTRNGVCKMCFGKDLATNRIVNIGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGVEDITGGFTRLIELIDAHEQP", "aa_hexdigest": "0d69966ffc331c42d09c365b161e2ab6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF05000.22", "name": "RNA_pol_Rpb1_4", "length": 56, "aa_cov": 0.20948616600790515, "hmm_cov": 0.9821428571428571, "evalue": 5.324132736482219e-12, "score": 41.05525588989258, "start": 1, "stop": 53 }, { "id": "PF04998.22", "name": "RNA_pol_Rpb1_5", "length": 195, "aa_cov": 0.7707509881422925, "hmm_cov": 0.6, "evalue": 5.408723111829805e-47, "score": 155.84100341796875, "start": 55, "stop": 249 } ], "seq_stats": { "molecular_weight": 27363.022100000013, "isoelectric_point": 9.110559272766114 }, "id": "GLJLFKCOHC_598", "locus": "GCA013368035_00582" }, { "type": "cds", "sequence": "contig_1", "start": 335438, "stop": 335593, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAGTATTAAACAATTAGGTTTTGACTATTCAACATATTCTGGAATTTCTATTTCGTTTAGTGATATTCTAGAAACTAATAAAAAGCAAGAATATATTGCTCAAGGTGATGAATATATTGCTAAATTAAAACACTACTATGAACAAGGTTGA", "aa": "MDSIKQLGFDYSTYSGISISFSDILETNKKQEYIAQGDEYIAKLKHYYEQG", "aa_hexdigest": "fd1d13a9a161c1bc127e024eb4652b75", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5917.4749, "isoelectric_point": 4.749659538269043 }, "id": "GLJLFKCOHC_599", "locus": "GCA013368035_00583" }, { "type": "cds", "sequence": "contig_1", "start": 335624, "stop": 336436, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04983.23" ], "nt": "ATGGCTGTTCACGTTCCTGTTTCTGAACAAGCATTGCTTGAAAGTCGTGAATTAATGTTGGCTAATAAAAACATTCTAGGACCTAAAGATGGTGAACCTATTATTAACCCTTCTCAAGATATGGTTTTAGGTATTTATTATCTAACTATTGAAGAACCAGGAGCTAAAGGTGAAGGACGTGTTTTTGATAACTATGAACACATGCTTAGAGCATTAGAAAATAAAGTAGTTACATTGCACACTAGAATAGCATTACCTGCTTCAGAAGTTAAAACTCCAAAACTTATTTTCCCATTTGATGGTCCTCAATATATAATTTCAACAGTAGGTAAATTTATTTTTAATAATATATTCCCTAAAACATTTCCATTTATTTACGACAATAAAGTTGCTAAGGCAAATTCATTGGAAGATTATAATCAAACATTCAATGCAAAATATGTCGTTCAAGCAGGTACTAATATTCCTGAATATATCAAAAATATGCCTATTGAAGAAGCATTTAACAAAAAGAATATTTCTAAAATAATACGTTATGTTTTTGATAAATATGTTGCTCAAATTTCAATGTCTGATATTGCAGAAGTTATTAATAATTTAACTGAATCAAGTGATGCTAATGATTCAATAATTCAATTCTTGCATGTTAAAAATTACGAAAATAGAAAAATTGATTATGAACATGCAATGTTGCTAGCTCAATTTACAAGTCAAGAAAAGCAAAAAATTGAAATGTTAAATCCTGCTAGGGCAAATGGACAAGAAGTTCCACTTAGTCCAGCTGAAAAAGCAGAAATTCTAGACACTGTATGA", "aa": "MAVHVPVSEQALLESRELMLANKNILGPKDGEPIINPSQDMVLGIYYLTIEEPGAKGEGRVFDNYEHMLRALENKVVTLHTRIALPASEVKTPKLIFPFDGPQYIISTVGKFIFNNIFPKTFPFIYDNKVAKANSLEDYNQTFNAKYVVQAGTNIPEYIKNMPIEEAFNKKNISKIIRYVFDKYVAQISMSDIAEVINNLTESSDANDSIIQFLHVKNYENRKIDYEHAMLLAQFTSQEKQKIEMLNPARANGQEVPLSPAEKAEILDTV", "aa_hexdigest": "e47c54f02340f394fb31d6537a96b9d7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04983.23", "name": "RNA_pol_Rpb1_3", "length": 182, "aa_cov": 0.6518518518518519, "hmm_cov": 0.8131868131868132, "evalue": 2.3729325640559815e-26, "score": 87.98047637939453, "start": 24, "stop": 199 } ], "seq_stats": { "molecular_weight": 30617.709800000026, "isoelectric_point": 5.372728538513184 }, "id": "GLJLFKCOHC_600", "locus": "GCA013368035_00584" }, { "type": "cds", "sequence": "contig_1", "start": 336692, "stop": 336967, "strand": "-", "frame": 1, "gene": null, "product": "DNA-directed RNA polymerase subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "nt": "TTGGTTATTCAAAACGAACTAAGAATGATTCAAGAAGCAGTAGATGCTTTAATTGATAACCAAAGAAGAACACCTAACCCTGTTTTATCAAAAGACAATCGTCCATTTAAATCAATTTCAGATGCATTAACTGGTAAAAAAGGTCGTTTTAGACAAAATTTACTAGGAAAACGTGTTGACTATTCTGGACGTTCAGTTATTGCCGTAGGGCCTTCATTAAAAATGCACCAATGTGGTATACCAAGAGAAATGGCTGCTAAATTATTTGAACCATGA", "aa": "MVIQNELRMIQEAVDALIDNQRRTPNPVLSKDNRPFKSISDALTGKKGRFRQNLLGKRVDYSGRSVIAVGPSLKMHQCGIPREMAAKLFEP", "aa_hexdigest": "4958223e7fd21991b5516508c96ea886", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002381C6D8", "query_cov": 0.9340659340659341, "subject_cov": 1.0, "identity": 0.624, "score": 112.0, "evalue": 1.02e-30, "valid": false, "uniref50_id": "UniRef50_A0A3B9S0R9" }, "pscc": { "uniref50_id": "UniRef50_A0A3B9S0R9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B9S0R9" ], "product": "DNA-directed RNA polymerase subunit" }, "genes": [], "id": "GLJLFKCOHC_601", "locus": "GCA013368035_00585" }, { "type": "cds", "sequence": "contig_1", "start": 336989, "stop": 337675, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "TTGCTTGGTTTAAAATCTTCAAATGGTTCTGACGTTGTTAAACGTGAAGAACTAGAAAATTTAATTTATTACAAAAATCATATTGTTCTAGAAGATGGTGGCTTAAAATCATTACCTAAAAATCAAATTATTGAAATCAATGAAGCAGCAATCATTTACCGCAATGCTCTAGAAGAATTACTTGAAAGATTTCAAGAAGGTACAGAAGAATATGATGATATTAAAGAAACTCTAGATTCTTTAATTGAAAAAGCAACATCAAAAATCGGCCAAGAATATGGAATTGATTTTTATGAATTAAACGAAGTAATTGAACATTATTCAGACGCTAAAATAGGTACTGGTGCTTTGGCTATTGAATATTTATTAAAAAACATAAATTTAGCTGAAGAAAAGCAAAAAGTAACTGATGAAATTAATCGTTTAAATGCAGAAGAAATTAATAATCCTGAACGTTTTGCCACAACTGCAAAACAAACAAGAGAAAAACTTTATAAACGTTTGCAAGTAATTAATGCATTTATTGAATCTAAACAAGATCCAACTAGCATGTTAATTTATAATTTGCCTGTTATTCCAGCAGATTTAAGACCTTTAATTCAATTAGATGGTGGTAGACACTCAACTAGCGATATTAATGAATTGTATCGTAGAGTAATTATTAGAAACAATAGATTGCAACAATGA", "aa": "MLGLKSSNGSDVVKREELENLIYYKNHIVLEDGGLKSLPKNQIIEINEAAIIYRNALEELLERFQEGTEEYDDIKETLDSLIEKATSKIGQEYGIDFYELNEVIEHYSDAKIGTGALAIEYLLKNINLAEEKQKVTDEINRLNAEEINNPERFATTAKQTREKLYKRLQVINAFIESKQDPTSMLIYNLPVIPADLRPLIQLDGGRHSTSDINELYRRVIIRNNRLQQ", "aa_hexdigest": "c57cfefce064fd45e8f993c30529a07d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 222, "aa_cov": 0.9342105263157895, "hmm_cov": 0.7207207207207207, "evalue": 1.144149100164434e-22, "score": 76.0958480834961, "start": 16, "stop": 228 } ], "seq_stats": { "molecular_weight": 26268.37680000003, "isoelectric_point": 4.939615440368651 }, "id": "GLJLFKCOHC_602", "locus": "GCA013368035_00586" }, { "type": "cds", "sequence": "contig_1", "start": 337709, "stop": 338068, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04997.17" ], "nt": "ATGAAAAAAATTTCAAAATACGAATCATTAGATGAAGAAAAAATTTCAAAAATTTCATTAGCATTAGCAACTCCTCAAGATGTTGAAACTTGGTCTCATGGTGAAGTTACTAAGCCAGAAACTATTAACTATAAATCATATAAGCCAGAACGTGGTGGATTATTTGATGAAATAATTTTTGGACCAATGATTGATTATCGTTGCCCTGTATGTGGTTTCAAATACAAAAAAGTTAATGAAGGAAGCTATTGTACAAGAACTGAATTATGTAAACAAGAACATGTTCAAATATTGCCTAAAATAGCACGTAGAACCCATATGGGACACATAAAACTAAATAGTCCAGTTGTTCACTTTTGA", "aa": "MKKISKYESLDEEKISKISLALATPQDVETWSHGEVTKPETINYKSYKPERGGLFDEIIFGPMIDYRCPVCGFKYKKVNEGSYCTRTELCKQEHVQILPKIARRTHMGHIKLNSPVVHF", "aa_hexdigest": "7ab28ebbdd5fde8231c32dc012a94f7b", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04997.17", "name": "RNA_pol_Rpb1_1", "length": 109, "aa_cov": 0.8991596638655462, "hmm_cov": 0.7522935779816514, "evalue": 5.078520935067719e-24, "score": 80.53942108154297, "start": 13, "stop": 119 } ], "seq_stats": { "molecular_weight": 13760.796499999986, "isoelectric_point": 8.857262992858885 }, "id": "GLJLFKCOHC_603", "locus": "GCA013368035_00587" }, { "type": "cds", "sequence": "contig_1", "start": 338070, "stop": 338240, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTAAACCAGGAACACCTGAATCGTTTAATGTTTTAGCATATGAATTACGTGGTTTAGGAATTAAATTAGAAGTACATCAAAAATCTGATAATGTTGTAGATGATTCAGAAGAAGATCAAAGAGTAGACACAAAATATTTCCAAGAATTTGAAGGGAGTTTAGAATAA", "aa": "MPKPGTPESFNVLAYELRGLGIKLEVHQKSDNVVDDSEEDQRVDTKYFQEFEGSLE", "aa_hexdigest": "b5604bd1c3019299f2f7444a7ee5c308", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6415.9671, "isoelectric_point": 4.457734489440917 }, "id": "GLJLFKCOHC_604", "locus": "GCA013368035_00588" }, { "type": "cds", "sequence": "contig_1", "start": 338352, "stop": 339254, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00562.33" ], "nt": "TTGGTTAAGGATGATGTTTATACATCGATTCACATTGAAGAACAATCAATTCAATTTAGACATTCAAAGGCTGGCGAAGATATTTTAACTGCAGATATTCCTAACGTTTCAAACTATTCTAAACGTTTCTTAGGTGAAAATGGAATTGTTAGAGTTGGTTCAGAAGTAACAGCAGGTGATATTTTAGTCGGAAGAACTTCTCCAAAAGGTGAAGAAAACCCAACTCCTGAAGAAAAATTAATGGCTGCAATTTTCGGTCAAAAAACTGCCTCAAGAAAAGATACTTCTCTAAAAGTAAAACATGGCCACAATGGAACCGTTGTTGCTGTTGATATTCTAAGTAGAGAATTTGGCGACCAATTAGAAGATGGCATTGAAAAAATAGTTAAGGTATCAATTGCTCAAAAACGTAAAATTCAAGTCGGCGACAAGATGGCTGGTCGTCACGGAAACAAAGGTGTTGTTTCAATAGTATTGCCTGTTGAAGAAATGCCTTATTTAGCAGATGGAACACCATTAGATATTGTTTTAAACCCTCAAGGTGTCCCTTCACGTATGAATATTGGTCAAGTTCTAGAATTGCACTTAGGACTTGCTGCTAAAAAATTAAATACAAAATTTGTTACACCAATATTTGATGGAATTTCACACAACCAAATTCGTGATATTCTTGAAGAAGCAAATATTGATGTAACAGGAAAATCAATAGTATACGATGGGGCCACTGGAGAACCATTTGATCATCCAATTTCTGTAGGGATTATGTACTATTTGAAGTTGTATCACATGGTTGATGACAAAATGCACGCACGTTCAGTAGGACCTTATTCATTGATTACCCAACAACCACTTGGTGGAAAGAGTCAAAATGGTGGTCAAAGATTTGGTGAAATGGAAACATGA", "aa": "MVKDDVYTSIHIEEQSIQFRHSKAGEDILTADIPNVSNYSKRFLGENGIVRVGSEVTAGDILVGRTSPKGEENPTPEEKLMAAIFGQKTASRKDTSLKVKHGHNGTVVAVDILSREFGDQLEDGIEKIVKVSIAQKRKIQVGDKMAGRHGNKGVVSIVLPVEEMPYLADGTPLDIVLNPQGVPSRMNIGQVLELHLGLAAKKLNTKFVTPIFDGISHNQIRDILEEANIDVTGKSIVYDGATGEPFDHPISVGIMYYLKLYHMVDDKMHARSVGPYSLITQQPLGGKSQNGGQRFGEMET", "aa_hexdigest": "a56dec150c290f662ff4229d0c0ca721", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0G1DPB0", "query_cov": 0.9666666666666667, "subject_cov": 0.8011049723756906, "identity": 0.5479999999999999, "score": 333.0, "evalue": 1.42e-110, "valid": false, "product": "DNA-directed RNA polymerase", "uniref50_id": "UniRef50_A0A660M9J4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 291, "aa_cov": 0.96, "hmm_cov": 0.9587628865979382, "evalue": 1.2645595701110977e-104, "score": 345.6925964355469, "start": 2, "stop": 289 } ], "seq_stats": { "molecular_weight": 32798.00410000002, "isoelectric_point": 6.073553276062011 }, "id": "GLJLFKCOHC_605", "locus": "GCA013368035_00589" }, { "type": "cds", "sequence": "contig_1", "start": 339297, "stop": 340502, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04565.21", "PFAM:PF10385.14", "PFAM:PF00562.33" ], "nt": "ATGTTGCATGACCAAAAAGTTTTGGTTTTAGGTAATGATCCTACATCAACAGAAAATTTCTTGTTGATTCCTGATTTAATTGCAACGATTTCGTATTATTTTAATTTACTTTCAAAGGTTGGGGTTGATGATGATCCTGACTCATTAGTTAATAAAAGAATTGTAACAATAGGCGAATTATTACAAAATCAATTTAGAATAGGGCTATTAAAATTAGAAAAAAATACAAGAGAAAGAATCTCGGCAAAAGATATTGATAAGATTACTGCTAAGAACGTTACAAACAATAAACCAATATTTAATCAATTTAAATCGTTCTTTAACACATCAAAACTTTCTCAATTTATGGATCAATGTAATCCTCTTGCTGAAATATCAAACAAGAGAAGAATAACCTCCCTAGGACCAAGAGGGTTGAACCGTGATACTGCACAATTTGAAGTTCGTGACGTTCACCCTACCCATTATGGAAGAATTTGTCCTATTGAAACACCCGAAGGACCAAACATTGGTTTAATTTTAAATTTAGCATCATTTGCTAAAATTGATAAATATGGTTTTATTCAATCACCATATTTCAGAGTAAATAAAAAAGTTGTAGATTTTTCAGAACCAGTTTACATTACTGCAATTGAAGAAACAGGATATACATTTGCACAATCAACTGTTCACATTGAAGGTAATGTAATTGTTGATGAAAAAGTAATGGTAAGACGTGACAATGAATATTTAGAAGTGAATGCTGAAGATGTAGATTTTATTGGTGTCTCAAACCGTCAAATGACCTCAATAGCTGCTTCGGCTATACCATTTTTGGAAAACGATGATGCTAACCGTGCATTGATGGGTTCTAACATGCAACGTCAAGCTGTTCCAGTGTTATTCCCAGAAGCTCCATTAGTTGCTACAGGAGTTGAAGCTGATATTGCTAAATATTCATCAACCAATATTAGAGCAACCAGATCAGGTGTTGTTAACTATGTAGATGCTAACGTTATTAAAGTAACTCCTGAAAATAGTTCAAAAAGTGATGTTTATCATTTAAGAACATTTGAAAGAAGTAACCAAGGTACATTAATTCACCAAGTACCTTTGGTTAAAGTTGGCCAACAAATTAATGCAGGAGATTTATTAGTTGATGGTCCTTCAATGAAAGATGGAGAATTAGCATTAGGAAAAAATGTTCTAGTAGGTTTTACTACTTGA", "aa": "MLHDQKVLVLGNDPTSTENFLLIPDLIATISYYFNLLSKVGVDDDPDSLVNKRIVTIGELLQNQFRIGLLKLEKNTRERISAKDIDKITAKNVTNNKPIFNQFKSFFNTSKLSQFMDQCNPLAEISNKRRITSLGPRGLNRDTAQFEVRDVHPTHYGRICPIETPEGPNIGLILNLASFAKIDKYGFIQSPYFRVNKKVVDFSEPVYITAIEETGYTFAQSTVHIEGNVIVDEKVMVRRDNEYLEVNAEDVDFIGVSNRQMTSIAASAIPFLENDDANRALMGSNMQRQAVPVLFPEAPLVATGVEADIAKYSSTNIRATRSGVVNYVDANVIKVTPENSSKSDVYHLRTFERSNQGTLIHQVPLVKVGQQINAGDLLVDGPSMKDGELALGKNVLVGFTT", "aa_hexdigest": "a5684565194a5de4b4ea6da7d1130d75", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04565.21", "name": "RNA_pol_Rpb2_3", "length": 69, "aa_cov": 0.17206982543640897, "hmm_cov": 0.9855072463768116, "evalue": 6.893045636762514e-30, "score": 98.50003814697266, "start": 114, "stop": 182 }, { "id": "PF10385.14", "name": "RNA_pol_Rpb2_45", "length": 67, "aa_cov": 0.16458852867830423, "hmm_cov": 0.9850746268656716, "evalue": 2.0817208341319013e-15, "score": 51.9917106628418, "start": 192, "stop": 257 }, { "id": "PF00562.33", "name": "RNA_pol_Rpb2_6", "length": 86, "aa_cov": 0.20448877805486285, "hmm_cov": 0.8837209302325582, "evalue": 1.7897654084159191e-10, "score": 35.854862213134766, "start": 319, "stop": 400 } ], "seq_stats": { "molecular_weight": 44613.200400000074, "isoelectric_point": 5.792484474182129 }, "id": "GLJLFKCOHC_606", "locus": "GCA013368035_00590" }, { "type": "cds", "sequence": "contig_1", "start": 340674, "stop": 341000, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF04561.19" ], "nt": "ATGTTTGGCGAAAATATTCCAGAATTGGAAGAAACATTTAAACGTGATAAAATCACTGGAATTAATGCAGAAGAAGTTACAAGGGAAGCACAAGAAGCAATTTATAGAATCATCAGAAAAGGCGATCGTATGACAGCTGATTCTGCTAGAAATTTAATTCCTACAACTTTGTTTAATGAAAAAAGATATAGTTTAACTGAAACAGGACGTTTCACTTTAAATAGAAAATTAAACATTATTGAAAGAATTGCTAATTCTTATTTAGCAGAAGATATTTTTTCTAGTGAAGGTGAACTTCTTTATGAAAAAGGAATGTTCATTACTTGA", "aa": "MFGENIPELEETFKRDKITGINAEEVTREAQEAIYRIIRKGDRMTADSARNLIPTTLFNEKRYSLTETGRFTLNRKLNIIERIANSYLAEDIFSSEGELLYEKGMFIT", "aa_hexdigest": "381928d4fce804669575b1b026c59d28", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04561.19", "name": "RNA_pol_Rpb2_2", "length": 71, "aa_cov": 0.6296296296296297, "hmm_cov": 0.9154929577464789, "evalue": 2.700223962829928e-06, "score": 22.57516098022461, "start": 27, "stop": 94 } ], "seq_stats": { "molecular_weight": 12558.086299999986, "isoelectric_point": 5.114622688293457 }, "id": "GLJLFKCOHC_607", "locus": "GCA013368035_00591" }, { "type": "cds", "sequence": "contig_1", "start": 341130, "stop": 341669, "strand": "-", "frame": 3, "gene": null, "product": "RNA polymerase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54", "UniRef:UniRef90_UPI002FDFDE54" ], "nt": "ATGGTGAATAAGGATAAATACGAAATTAGAAAATTTGGACCTATTACCCTAAGACGTGACTATTCAATTAGTCAAAAGAAATTTGAAACTCCTGACTTTCTTGAAATGCAACGTGAATCAGTTGAAAAATTTCTAACAGTTGGAATTGAAGAAGAATTAAGAAATATCTATCCAATTGAAGCACATGGAAAAGTTAGAATTGAATACATTCATAATTCAGCACATTTTGAATATCCTAAAAAAACTGAATATGAAAGCATTAAAGAAGCTAAACAAAAAGGTTCATCATATCAAGGAAAGTTAAAAGCGCACCTAAGACAAATCAATATCGAAACAGGTGAAGTTGAAGATTCAGAAGTAGTATTTGCTGAAATTCCTATAATGACTTATGGTGGATCATTTATTATCAATGGTTCAGAAAAAGTTATTGTTTCGCAATTAATTAGATCTACCGGGGCTTATTTTGGAATTAATGTTAGAAATAAACAAGCCAACGACTTGTTTAATAAAGTAGAAATTATTCCTCAAATTGGTTCATGA", "aa": "MVNKDKYEIRKFGPITLRRDYSISQKKFETPDFLEMQRESVEKFLTVGIEEELRNIYPIEAHGKVRIEYIHNSAHFEYPKKTEYESIKEAKQKGSSYQGKLKAHLRQINIETGEVEDSEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGINVRNKQANDLFNKVEIIPQIGS", "aa_hexdigest": "73e8e121e79577765c555dbc7556d4bc", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 340827, "stop": 341660, "observations": { "mutation": [ 341132 ], "stop": [ 341132 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FDFDE54", "query_cov": 0.9832402234636871, "subject_cov": 0.5482866043613707, "identity": 0.8859999999999999, "score": 307.0, "evalue": 4.96e-103, "gene_start": 4, "gene_end": 179, "reference_start": 3, "reference_end": 178, "reference_sequence": "MVKAKYELRKFGPITERRDYSISQKKFATPDFLEMQRTSVENFIHQGIEEELRNIYPIESHGKVRIEYVWNSAHFDYPKKSEFESIKEAKQKGSSYQGKLKARLRQINLETGEVEESEVVFAEIPIMTYGGSFIINGSEKVIVSQLIRSTGAYFGLNVRNKQANDLFNKVEIIPQVGSWVEIYHKVTSANADSVKIHIDKNKSFLLTTFLKALGFSETGIRAMFGDIAELEETLKKDKIVGLSLEESTREAQEAIYRIIRKGDRMTVESARNLIPSTLFNEKRYNLTQIGRFTLNRKLNVIERIANSFLAESIFSDKGELL" }, "paralog": false, "identity": 0.851985559566787, "score": 465.0, "evalue": 1.08e-167, "description": "Internal stop codon at 341132. Nonsense mutation around 341132.", "psc": { "uniref90_id": "UniRef90_UPI002FDFDE54", "product": "RNA polymerase beta subunit", "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FDFDE54", "UniRef:UniRef50_UPI002FDFDE54" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FDFDE54", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FDFDE54" ] } }, "id": "GLJLFKCOHC_608", "locus": "GCA013368035_00592" }, { "type": "cds", "sequence": "contig_1", "start": 341803, "stop": 342177, "strand": "-", "frame": 2, "gene": "rplL", "product": "50S ribosomal protein L7/L12", "db_xrefs": [ "COG:COG0222", "COG:J", "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625", "RefSeq:WP_012855544.1", "SO:0001217", "UniParc:UPI00000BF3D8", "UniRef:UniRef100_D1J856", "UniRef:UniRef50_B3PM75", "UniRef:UniRef90_B3PM75" ], "nt": "ATGGCAAAATTAACAAAAGAAGAATTTGTTTCAGCATTAAAAGAAATGAACATTAAAGAAGTTATGGAATTAATTCAAGGATTAAAAGAAGAATTTGGAATTGATCCAACAGCAGTTGTAGCAGCAGCAGCTCCAGCAGCAGCAGCTGACGCAGCAGAAGAAAAAACTGTATTTAATGTTACTTTAAAATCAGATGGTGGAAACAAGCTAGCAGTTATTAAAGTTGTTAAAGACTTATTAGGTCTAGGTTTAATGGATGCTAAAAAACTTGTTGAATCAGCACCTGTTGTAATTAAAGAAAATGCTAAAAAAGAAGAAGCTGAAGAACTAAAAGCTAAATTAACAGAAGCTAAAGCCGAAGTTACTTTAGACTAA", "aa": "MAKLTKEEFVSALKEMNIKEVMELIQGLKEEFGIDPTAVVAAAAPAAAADAAEEKTVFNVTLKSDGGNKLAVIKVVKDLLGLGLMDAKKLVESAPVVIKENAKKEEAEELKAKLTEAKAEVTLD", "aa_hexdigest": "53b91ce3f730c59f29e8ab79a1e81e35", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000BF3D8", "ncbi_nrp_id": "WP_012855544.1", "uniref100_id": "UniRef100_D1J856", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BF3D8", "RefSeq:WP_012855544.1", "UniRef:UniRef100_D1J856" ] }, "ips": { "uniref100_id": "UniRef100_D1J856", "uniref90_id": "UniRef90_B3PM75" }, "psc": { "uniref90_id": "UniRef90_B3PM75", "gene": "rplL", "product": "50S ribosomal protein L7/L12", "uniref50_id": "UniRef50_B3PM75", "cog_id": "COG0222", "cog_category": "J", "go_ids": [ "GO:0003729", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_B3PM75", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PM75" ], "product": "Large ribosomal subunit protein bL12" }, "genes": [ "rplL" ], "id": "GLJLFKCOHC_609", "locus": "GCA013368035_00593" }, { "type": "cds", "sequence": "contig_1", "start": 342200, "stop": 342697, "strand": "-", "frame": 1, "gene": "rplJ", "product": "50S ribosomal protein L10", "db_xrefs": [ "COG:COG0244", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02864", "RefSeq:WP_012855545.1", "SO:0001217", "UniParc:UPI0001BC223D", "UniRef:UniRef100_D1J857", "UniRef:UniRef50_A0A448ZYM3", "UniRef:UniRef90_D1J857" ], "nt": "ATGAGCGCATTAAAAGATGCCAAGGTTTTAGTTGTTGAAGAAATTTCAAACAATTTAAAAGAATCACAAGCATTATATGTAATTAATTATGCTACTTTGGATGTTGTTTCATTTCAAGAAATTAGAAAAGAATTAGCTCCAAATAATGCATTGCTAAAAGTTTACAAAAATAGACTAGTTAAACAAGCATTAAAAAATACAGAATATGCAAATATTAATGATAGTTTAGTTTTACAAAATGCTTATGCCTTTGTTAAAGGCGATTCATTAGCAGCATTAAAAACCTTAGTAGGCTTAAAGAAAAAATTCCCTGCATTAAGAATTGTAAGTGGAATTTATGAAAATAAAGTAGTTGATGAAAAAACATTAGACGAAATATCAAAACTTCCTTCATTTACAGAATCACTTATGATTCTTGGAAATTCATTATTGAGTCCATTGAAACAATTATCAATTGGTTTAAATGAATTAATAAAACAAGGAAAAATTTCAGAATAA", "aa": "MSALKDAKVLVVEEISNNLKESQALYVINYATLDVVSFQEIRKELAPNNALLKVYKNRLVKQALKNTEYANINDSLVLQNAYAFVKGDSLAALKTLVGLKKKFPALRIVSGIYENKVVDEKTLDEISKLPSFTESLMILGNSLLSPLKQLSIGLNELIKQGKISE", "aa_hexdigest": "4e952e6b06822b7a4ca63497eef65389", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC223D", "ncbi_nrp_id": "WP_012855545.1", "uniref100_id": "UniRef100_D1J857", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC223D", "RefSeq:WP_012855545.1", "UniRef:UniRef100_D1J857" ] }, "ips": { "uniref100_id": "UniRef100_D1J857", "uniref90_id": "UniRef90_D1J857" }, "psc": { "uniref90_id": "UniRef90_D1J857", "gene": "rplJ", "product": "50S ribosomal protein L10", "uniref50_id": "UniRef50_A0A448ZYM3", "cog_id": "COG0244", "cog_category": "J", "kegg_orthology_id": "K02864", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZYM3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZYM3" ], "product": "Large ribosomal subunit protein uL10" }, "genes": [ "rplJ" ], "id": "GLJLFKCOHC_610", "locus": "GCA013368035_00594" }, { "type": "rRNA", "sequence": "contig_1", "start": 342917, "stop": 345812, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9900854700854701, "score": 2768.2, "evalue": 4.6e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAACGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTGCAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGTAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGATGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTTATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "GLJLFKCOHC_39", "locus": "GCA013368035_00595" }, { "type": "rRNA", "sequence": "contig_1", "start": 345995, "stop": 347512, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1513.4, "evalue": 2.4e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGTAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "GLJLFKCOHC_35", "locus": "GCA013368035_00596" }, { "type": "cds", "sequence": "contig_1", "start": 347898, "stop": 348095, "strand": "-", "frame": 3, "gene": "rpmF", "product": "50S ribosomal protein L32", "db_xrefs": [ "COG:COG0333", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855546.1", "SO:0001217", "UniParc:UPI0000DB8019", "UniRef:UniRef100_D1J858", "UniRef:UniRef50_Q4A6N8", "UniRef:UniRef90_D1J858" ], "nt": "ATGGCAGTAGTACCAAAAAGAAAAACTTCAAAACAAAGAAAACATTTAAGAAGATCACACCACGCTCTAGTGGCTCCAACATTAGTTGAATGTTCACAATGTAAGAATTTAATTACTCCTCACCAAGCATGTGAAAACTGCGGTTTTTATAGAGGAAGAAAAGTTATTAAAGAAGCAATTAACGATAAAATTAAATAA", "aa": "MAVVPKRKTSKQRKHLRRSHHALVAPTLVECSQCKNLITPHQACENCGFYRGRKVIKEAINDKIK", "aa_hexdigest": "2966d01971762bee811933c45cb0dcc6", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0000DB8019", "ncbi_nrp_id": "WP_012855546.1", "uniref100_id": "UniRef100_D1J858", "db_xrefs": [ "SO:0001217", "UniParc:UPI0000DB8019", "RefSeq:WP_012855546.1", "UniRef:UniRef100_D1J858" ] }, "ips": { "uniref100_id": "UniRef100_D1J858", "uniref90_id": "UniRef90_D1J858" }, "psc": { "uniref90_id": "UniRef90_D1J858", "gene": "rpmF", "product": "50S ribosomal protein L32", "uniref50_id": "UniRef50_Q4A6N8", "cog_id": "COG0333", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A6N8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A6N8" ], "product": "Large ribosomal subunit protein bL32" }, "genes": [ "rpmF" ], "id": "GLJLFKCOHC_611", "locus": "GCA013368035_00597" }, { "type": "cds", "sequence": "contig_1", "start": 348120, "stop": 348407, "strand": "-", "frame": 3, "gene": null, "product": "Large ribosomal subunit protein bL17", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "nt": "TTGATTAATGGCAAAGTAACAACCACATTAGAACGTGCTAAAAGAATTCGTTCAAATGCAGAAAAAATGATTACTCTAGGTAAGAAAAATACCCTAGCTTCGCGTAGACAAGCAGCCAAATATTTAAGATTAATTGCAACAGAAAACAAAAATAAAAATTCATTACAATATTTATTTGATGTTGTGGCACCAAAATACGTTGAACGTAATGGTGGATATACAAGAATTACAAAATTAGCCAATCGTGCTGGTGATAATGCTAAAATGGCAATAATCGAATTGGTATAA", "aa": "MINGKVTTTLERAKRIRSNAEKMITLGKKNTLASRRQAAKYLRLIATENKNKNSLQYLFDVVAPKYVERNGGYTRITKLANRAGDNAKMAIIELV", "aa_hexdigest": "624475da950116ad9799080f8a5457a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001FA24CA4", "query_cov": 1.063157894736842, "subject_cov": 0.808, "identity": 0.564, "score": 107.0, "evalue": 2.53e-28, "valid": false, "gene": "rplQ", "product": "50S ribosomal protein L17", "uniref50_id": "UniRef50_P20277", "kegg_orthology_id": "K02879", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P20277", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P20277" ], "product": "Large ribosomal subunit protein bL17" }, "genes": [], "id": "GLJLFKCOHC_612", "locus": "GCA013368035_00598" }, { "type": "cds", "sequence": "contig_1", "start": 348554, "stop": 349522, "strand": "-", "frame": 1, "gene": "rpoA", "product": "DNA-directed RNA polymerase subunit alpha", "db_xrefs": [ "COG:COG0202", "COG:K", "EC:2.7.7.6", "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983", "SO:0001217", "UniRef:UniRef50_P38018", "UniRef:UniRef90_D1J860" ], "nt": "ATGAAGAAAATACAAAAAATTACATATAAAGAATTAGTAGCAGAAAAAGTATCAGATTTTAATACAACATTCGTTATTGAACCACTAATGCGTGGTTATGGTAACACAATAGGAACTGTAATAAGAAGAACATTACTTTCTTCGATTACTTCTGTTGCTCCATTTGCAATAAAAATTAGTCATGTTGAACATGAATTTACTGCAATTGAAGGATTAAGAGAAGATGCTATTACATTAGTTGCAAACATAAGAAAAATTCGCTTTGCATATGACCCAGAATTATTTGAAAAAGATAATTTAGCTAAAATTTCATTTAAATCAACAAAAGAAGGGGAAGTTCATGCTTCGGATATTGAAACTATCCCTGGACTTGACATTGTTAATAAAGATCAATACATAGCAACAATATCAAAAAATGGTTCATTGGAATTTGAATTATATTTAAGAACCGGTAGAGGCTATGTTTCATTTGAAGACAATAAAAAAACCATTCTAGAATATGGTCCAAAATTAGAATCAAAAATCAAAAATGGTCAATTTTTGGCAATGGATAGTGACTTCAGTCCTATTAAAAAAGTTGCGATTTTATTTGAAGAATTAAATTCTACTTCTAACATAATTGAAGAAAGATTAAAAATTAAAGTTGAAACCGATGGAACTGTTGAAGCTAAAAACGTTATGGAAGAAGCAGCAAAGATTATAGTTGCTCATTTCCAAATCATTGGAAACATTGACGCATTAGGAACAATTGATTTATTCGATGATCAAAAAGAAAAACATGAAAAAACTCCAAAAGTTTCAGTTTCTATTGATAAATTGAATTTGACAATTCGTTCATTGAATGCCTTAAGACGTGCTGGATTTAATAATGTTGATGAAATAATGAAATTAAGCGATGAAGAACTTTCGAATATTAAAAATTTGGGTAAAAAATCAGTACAAGATATTATTGACCGTCGTAGAGAATGA", "aa": "MKKIQKITYKELVAEKVSDFNTTFVIEPLMRGYGNTIGTVIRRTLLSSITSVAPFAIKISHVEHEFTAIEGLREDAITLVANIRKIRFAYDPELFEKDNLAKISFKSTKEGEVHASDIETIPGLDIVNKDQYIATISKNGSLEFELYLRTGRGYVSFEDNKKTILEYGPKLESKIKNGQFLAMDSDFSPIKKVAILFEELNSTSNIIEERLKIKVETDGTVEAKNVMEEAAKIIVAHFQIIGNIDALGTIDLFDDQKEKHEKTPKVSVSIDKLNLTIRSLNALRRAGFNNVDEIMKLSDEELSNIKNLGKKSVQDIIDRRRE", "aa_hexdigest": "87e58c6c79dce2574865c9b0167ca5f9", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J860", "query_cov": 1.0, "subject_cov": 0.936046511627907, "identity": 0.997, "score": 608.0, "evalue": 4.63e-219, "valid": true, "gene": "RpoA", "product": "DNA-directed RNA polymerase subunit alpha", "ec_ids": [ "2.7.7.6" ], "uniref50_id": "UniRef50_P38018", "cog_id": "COG0202", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0003899", "GO:0006351", "GO:0046983" ] }, "pscc": { "uniref50_id": "UniRef50_P38018", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38018" ], "product": "DNA-directed RNA polymerase subunit alpha" }, "genes": [ "rpoA" ], "id": "GLJLFKCOHC_613", "locus": "GCA013368035_00599" }, { "type": "cds", "sequence": "contig_1", "start": 349816, "stop": 349926, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAGAAAAATAAAAAAGTTATTACTCAAGGTATTGCACATATTCACTCAACTTACCAAAACACAATTGTTTCATTCTCAGATCTAAAAGGAAATGTTTTTGCTTGA", "aa": "MAKKNKKVITQGIAHIHSTYQNTIVSFSDLKGNVFA", "aa_hexdigest": "a34c7eb380a59aa586242df175c0d8bb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3990.5867000000007, "isoelectric_point": 10.000351905822754 }, "id": "GLJLFKCOHC_614", "locus": "GCA013368035_00600" }, { "type": "cds", "sequence": "contig_1", "start": 349929, "stop": 350297, "strand": "-", "frame": 3, "gene": "rpsM", "product": "30S ribosomal protein S13", "db_xrefs": [ "COG:COG0099", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02952", "RefSeq:WP_012855550.1", "SO:0001217", "UniParc:UPI0001BC2240", "UniRef:UniRef100_D1J862", "UniRef:UniRef50_P38017", "UniRef:UniRef90_D1J862" ], "nt": "ATGGCCAGAGTTTTAAACATTGAAATTCCTAATAATAAAAAAGTTCGTATTTCACTTACTTACATCTTTGGTATTGGCGTTTCACTAGCAAAAGAAATTTTAAAAGATGCTAATGTTGACGGCGAAAAAAGAGTTAAAGATCTTAGCGAAGAAGAATTAACTAGAATCAGAGATGAAGCTAAAAAATACACCACTGAAGGTGATTTAAGAAGAGAAATAAATCTAAACATTAAAAGATTAATGGAAATTAAATCATATCGTGGTATTAGACACCGTAAGGGATTACCTGTACGTGGTCAATCTACAAAGAAAAATGCAAGAACAAGAAAAGGTCCAAGAAAAACAATAGCAGGAAAGAAAGGTAAATAA", "aa": "MARVLNIEIPNNKKVRISLTYIFGIGVSLAKEILKDANVDGEKRVKDLSEEELTRIRDEAKKYTTEGDLRREINLNIKRLMEIKSYRGIRHRKGLPVRGQSTKKNARTRKGPRKTIAGKKGK", "aa_hexdigest": "ac04cc895c8f114982ffe618d49356fa", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0001BC2240", "ncbi_nrp_id": "WP_012855550.1", "uniref100_id": "UniRef100_D1J862", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2240", "RefSeq:WP_012855550.1", "UniRef:UniRef100_D1J862" ] }, "ips": { "uniref100_id": "UniRef100_D1J862", "uniref90_id": "UniRef90_D1J862" }, "psc": { "uniref90_id": "UniRef90_D1J862", "gene": "rpsM", "product": "30S ribosomal protein S13", "uniref50_id": "UniRef50_P38017", "cog_id": "COG0099", "cog_category": "J", "kegg_orthology_id": "K02952", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_P38017", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P38017" ], "product": "Small ribosomal subunit protein uS13" }, "genes": [ "rpsM" ], "id": "GLJLFKCOHC_615", "locus": "GCA013368035_00601" }, { "type": "cds", "sequence": "contig_1", "start": 350426, "stop": 350641, "strand": "-", "frame": 1, "gene": "infA", "product": "translation initiation factor IF-1", "db_xrefs": [ "COG:COG0361", "COG:J", "GO:0003743", "GO:0006413", "KEGG:K02518", "RefSeq:WP_012855552.1", "SO:0001217", "UniParc:UPI0001BC2242", "UniRef:UniRef100_D1J864", "UniRef:UniRef50_Q4A5I6", "UniRef:UniRef90_D1J864" ], "nt": "ATGGCAAAAGACGCAATCAAAATGTCAGGCAAAATTACTAAAATGCACACATCAAGAGACTATGATGTTTTATTAGAAAATGGGCTTACTATTAAAGCTTCTATTTCAGGTAAAATGTCACTTCACAATATCAAAATGATCCCTGGGGATTTAGTTGATGTTGAACTTAGTCCATATGATATGAGCAGAGGTAGAATTGTTTTTAGACACAAATAA", "aa": "MAKDAIKMSGKITKMHTSRDYDVLLENGLTIKASISGKMSLHNIKMIPGDLVDVELSPYDMSRGRIVFRHK", "aa_hexdigest": "00b621bb30ccf8e19c9059b63d700fad", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2242", "ncbi_nrp_id": "WP_012855552.1", "uniref100_id": "UniRef100_D1J864", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2242", "RefSeq:WP_012855552.1", "UniRef:UniRef100_D1J864" ] }, "ips": { "uniref100_id": "UniRef100_D1J864", "uniref90_id": "UniRef90_D1J864" }, "psc": { "uniref90_id": "UniRef90_D1J864", "gene": "infA", "product": "translation initiation factor IF-1", "uniref50_id": "UniRef50_Q4A5I6", "cog_id": "COG0361", "cog_category": "J", "kegg_orthology_id": "K02518", "go_ids": [ "GO:0003743", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5I6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5I6" ], "product": "Translation initiation factor IF-1" }, "genes": [ "infA" ], "id": "GLJLFKCOHC_616", "locus": "GCA013368035_00602" }, { "type": "cds", "sequence": "contig_1", "start": 350740, "stop": 351393, "strand": "-", "frame": 2, "gene": "map", "product": "type I methionyl aminopeptidase", "db_xrefs": [ "COG:COG0024", "COG:J", "EC:3.4.11.18", "GO:0006508", "GO:0070006", "SO:0001217", "UniRef:UniRef50_L0RW44", "UniRef:UniRef90_D1J865" ], "nt": "ATGGTCATAATAAAAAATCAATTTGAAATTGAAAAGATCAAAAAAGCTTGTAAAATCCTGGCAGAAGTGAAAACTGTTTTATACGACTTTATAAGACCAGGCGTTTCTTTAAAAGAAATTGATAGCGTAGCTTTTAAAGAAATAAGAAAAAGGGGTGGAAAACCAGCATTCTTAGGACAATATGGTTTTCCCAATACATGTTGCATTTCTGTTAATAATGAATTAATTCATGGAATTCCTAGTGATTATGTTGTAAAGGATGGAGATATAGTCAAAATTGACACTGGAGCAATTTATGACGGTTATTATTCTGATTCAGCATTTACAAAAGGAGTTGGCAACATAACTGAAGCAGACAAAAAATTAATTGCCTGCGCAAAAGACGCTTTTTATGCCGGCTTTAATGCAATTAAAGTTGGCAAACACATTGGTGATATTTCTGCAGCCATAGGAAATTTAATTCGTGATAGAGGATATTTTACTCCAGATGAATATACAGGTCACGGAATAGGCAGAAAGTTGCATGAAGATCCATATGTTTATAACGATGGCTATCCAGGAACTGGACAGCTAATTAGAAATGGTATGGTAATTTGCATTGAACCAATGATTTTACAAAAATCTAAAAATGTATTGGTCAAAGGAAATGGATGA", "aa": "MVIIKNQFEIEKIKKACKILAEVKTVLYDFIRPGVSLKEIDSVAFKEIRKRGGKPAFLGQYGFPNTCCISVNNELIHGIPSDYVVKDGDIVKIDTGAIYDGYYSDSAFTKGVGNITEADKKLIACAKDAFYAGFNAIKVGKHIGDISAAIGNLIRDRGYFTPDEYTGHGIGRKLHEDPYVYNDGYPGTGQLIRNGMVICIEPMILQKSKNVLVKGNG", "aa_hexdigest": "f0771c603bdc7f9bf4e85fd5e65ecb54", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_D1J865", "query_cov": 1.0, "subject_cov": 0.868, "identity": 1.0, "score": 442.0, "evalue": 1.02e-156, "valid": true, "gene": "map", "product": "type I methionyl aminopeptidase", "ec_ids": [ "3.4.11.18" ], "uniref50_id": "UniRef50_L0RW44", "cog_id": "COG0024", "cog_category": "J", "go_ids": [ "GO:0006508", "GO:0070006" ] }, "pscc": { "uniref50_id": "UniRef50_L0RW44", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RW44" ], "product": "Methionine aminopeptidase" }, "genes": [ "map" ], "id": "GLJLFKCOHC_617", "locus": "GCA013368035_00603" }, { "type": "cds", "sequence": "contig_1", "start": 351384, "stop": 352079, "strand": "-", "frame": 3, "gene": "adk", "product": "adenylate kinase", "db_xrefs": [ "COG:COG0563", "COG:F", "EC:2.7.4.3", "GO:0005524", "GO:0006139", "GO:0019205", "RefSeq:WP_114119156.1", "SO:0001217", "UniParc:UPI000DED5356", "UniRef:UniRef100_UPI000DED5356", "UniRef:UniRef50_Q6KI35", "UniRef:UniRef90_D1J866" ], "nt": "ATGATAGAAAAATCTCAAGAAGTGTACAACGAACACACAAAAAAATTTCCTAATATAATTATGCTTGGCGCACCAGGAGCTGGTAAAGGCTCTATTGCAACAAAAATGGTGCAAAATAATGATTATGTTCAAATTTCAACTGGGGACATGTTTAGACAAGAAATTAAAAATCAAACTCCCCTTGGCTTAAAAATAAAATCTATTTTAGATTCGGGAGCATATGTTGATGATTCTATAACAAATCAACTAGTTGAAAAAAAATTAACTGAATTAATAAGAGAAAAGAAACACTTCATATTAGACGGATATCCCCGCACATTAGCTCAAGCAAAGTTTTTACTTTCATTAGCAAATGTTGGAATTAAAATTGATAAAGTAATTTTGCTAGAAATTACTGATGAACAAATTATTGACCGTCTTTCAAAACGTAGAATTTGTTTAAATTGTAAAACCATTTATCATTTGCAATCATTTCCACCATTACCTGGTGATTTGTGCATTAAATGCCACGAAAAAGTAACAAAAAGACCAGATGACGAACCAGAAGTAATAAAGAAACGTTTATCAATATATAATGAACAAACTAAATGTTTGATCAATTTTTATCAAGAACTTGGTATACTTTTAAAGATAAATAGTTATCAAACAATTGATAAAGTATATAATGATGTAGAAGAGGCTTTAAAATGGTCATAA", "aa": "MIEKSQEVYNEHTKKFPNIIMLGAPGAGKGSIATKMVQNNDYVQISTGDMFRQEIKNQTPLGLKIKSILDSGAYVDDSITNQLVEKKLTELIREKKHFILDGYPRTLAQAKFLLSLANVGIKIDKVILLEITDEQIIDRLSKRRICLNCKTIYHLQSFPPLPGDLCIKCHEKVTKRPDDEPEVIKKRLSIYNEQTKCLINFYQELGILLKINSYQTIDKVYNDVEEALKWS", "aa_hexdigest": "d8f4f095e0d824937107c40fe2a7a904", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI000DED5356", "ncbi_nrp_id": "WP_114119156.1", "uniref100_id": "UniRef100_UPI000DED5356", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DED5356", "RefSeq:WP_114119156.1", "UniRef:UniRef100_UPI000DED5356" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DED5356", "uniref90_id": "UniRef90_D1J866" }, "psc": { "uniref90_id": "UniRef90_D1J866", "gene": "Adk", "product": "adenylate kinase", "ec_ids": [ "2.7.4.3" ], "uniref50_id": "UniRef50_Q6KI35", "cog_id": "COG0563", "cog_category": "F", "go_ids": [ "GO:0005524", "GO:0006139", "GO:0019205" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KI35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KI35" ], "product": "Adenylate kinase" }, "genes": [ "adk" ], "id": "GLJLFKCOHC_618", "locus": "GCA013368035_00604" }, { "type": "cds", "sequence": "contig_1", "start": 352076, "stop": 352507, "strand": "-", "frame": 1, "gene": "secY", "product": "SecY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78", "UniRef:UniRef90_UPI0021BD3D78" ], "nt": "TTGCAATTTACTCAACCACTTGGATTCTTTTTATTTATTTTAATTACATTTGGATTAACAATATTAATGGGAATCCAACAATCAAGAATTGATAAAATTAGTGAAGATTTTGCTAAAAATAGTACTTTTATTCCTGGCGTTAGACCCGGAGAACAAACAGAAGATTATTTATTGAATGTAATTTTACGTTTATCATTCTTTTCTTCAGGATATTTATTGTTGCTAGGCGCTTTACAATTTATTCAACAAATGCTAGGCATGCCAGCCTCAATAGCATTTGGCGGTACAAGTATAATGATACTAGTTTCAACGGCTTATGAAACTGTTCAACAAATTAAAGCCCGCTACAAGTCGCAAGAATTATCGAGAAAGAGAAAAATGATAAGAGAATTAAAAGAAATGTATGGAGAGGAAGAAGAAGATTTAATATGA", "aa": "MQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLI", "aa_hexdigest": "85abb75e04e5391b92af8f76c1e9a417", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "secY" ], "truncated": "3-prime", "pseudogene": { "start": 352076, "stop": 352807, "observations": { "mutation": [ 352522 ], "stop": [ 352522 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0021BD3D78", "query_cov": 1.0, "subject_cov": 0.5296296296296297, "identity": 0.867, "score": 243.0, "evalue": 5.13e-79, "gene_start": 1, "gene_end": 143, "reference_start": 127, "reference_end": 269, "reference_sequence": "MLWFFVGIASGLIPSFKSAFQYYIPSAKQTNLVLQEIINYAVYILVYLLTIFIVVIFTIAERRIPIQQVGAGLSKNAEELSYLPLKANPAGIMSVIFSMMILGVPTMIANMLNPNTSRYYQWVYANLQFTQPLGFFLFILITFGLTILMGIQQSKIDKISEDFAKSSTYIPGIRPGEQTETYLLNVVLRLSFFSSGFLIVLGSLQFVQQMIGMPASIAFGGTTVMILVSTAYETIQQIKARYKSQELARKRRTIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.8559670781893004, "score": 414.0, "evalue": 5.25e-149, "description": "Internal stop codon at 352522. Nonsense mutation around 352522.", "psc": { "uniref90_id": "UniRef90_UPI0021BD3D78", "product": "SecY", "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0021BD3D78", "UniRef:UniRef50_UPI0021BD3D78" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0021BD3D78", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BD3D78" ] } }, "id": "GLJLFKCOHC_619", "locus": "GCA013368035_00605" }, { "type": "cds", "sequence": "contig_1", "start": 352520, "stop": 353293, "strand": "-", "frame": 1, "gene": "secY", "product": "preprotein translocase subunit SecY", "db_xrefs": [ "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952", "SO:0001217", "UniRef:UniRef50_A0A448ZXS5", "UniRef:UniRef90_D1J867" ], "nt": "TTGAATTTAGTAGGTGGCGGTGGATTAAGACAATTCTCTGTTGTCGCGCTAGGAATAGGACCGTTTATATCATCTAGTTTAGTTATGATGATATTGCAAACTAAAGCATTCCCGGCCATTCACCGTTTAAGTCAATCTGGTCCTCAAGGCCGGATTAAAATAAATTTCATTACTTATGGAGTTACTTTTGTTTTTGCAATAGTTCAAGCATATTTGATAACAAAAGCATTAGTTAATCCTCGTCAAGGATTTGGAATTACTTTTTCACCAAAATTAGAAAAATTATTTGGACAAAGTGGAATGGTAGCATATCAATATTTCATTTTGCCTGCAATATTAGTAGCTGGCTCATTCTTTTCATTGTTCTTATCAGAACAAATAACTAATAGAGGCGTTGGCAATGGAACGAGTTTAATAATCTTTGTTGGTATAGCAAGTGCTTTAATTCCTTCATTCAAGAGCGCGTTTAAATTTTATGTTCCATCAGCTCAACAAAATAATTTAGTTCTACAAGAAGCAATAAATTACGCAGTATATATCTTTTGCTATTTACTTGTAATATTTATTGTTGTAATATTTACAATTGCAGAAAGACATATTCCAATTCAACAAGTTGGTGCGGGTCTTTCTAAAAACGAAAAAGAATTGAGTTATTTACCTATTAAAGCAAATCCTGCTGGTATCATGTCAGTTATCTTCTCAATGATGATATTGAGTGTGCCAACAATGATTGCAAACTTATTTGATCCAAATACGAGCAAGTATTATCAATGA", "aa": "MNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQ", "aa_hexdigest": "36e165d8b8faf321a6d6ce6ece0a03ce", "start_type": "TTG", "rbs_motif": "AGGAG(G)/GGAGG", "genes": [ "secY" ], "truncated": "both", "pseudogene": { "start": 352217, "stop": 353542, "observations": { "mutation": [ 352522, 353434, 353437, 353446 ], "stop": [ 352522, 353434, 353437, 353446 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J867", "query_cov": 1.0, "subject_cov": 0.5255623721881391, "identity": 0.996, "score": 489.0, "evalue": 4.52e-171, "gene_start": 1, "gene_end": 257, "reference_start": 84, "reference_end": 340, "reference_sequence": "MARKTKLEKEISQEKIERKLALDKFLVDKKNTWNEWWKNHNLFKKILFTLFIITLFLATGTITIPGVNLANPDSLTQGDFFGILNLVGGGGLRQFSVVALGIGPFISSSLVMMILQTKAFPAIHRLSQSGPQGRIKINFITYGVTFVFAIVQAYLITKALVNPRQGFGITFSPKLEKLFGQSGMVAYQYFILPAILVAGSFFSLFLSEQITNRGVGNGTSLIIFVGIASALIPSFKSAFKFYVPSAQQNNLVLQEAINYAVYIFCYLLVIFIVVIFTIAERHIPIQQVGAGLSKNEKELSYLPIKANPAGIMSVIFSMMILSVPTMIANLFDPNTSKYYQWVYNNLQFTQPLGFFLFILITFGLTILMGIQQSRIDKISEDFAKNSTFIPGVRPGEQTEDYLLNVILRLSFFSSGYLLLLGALQFIQQMLGMPASIAFGGTSIMILVSTAYETVQQIKARYKSQELSRKRKMIRELKEMYGEEEEDLIW" }, "paralog": false, "identity": 0.9909297052154195, "score": 839.0, "evalue": 2.46e-311, "description": "Internal stop codon at 352522, 353434, 353437, 353446. Nonsense mutation around 352522, 353434, 353437, 353446.", "psc": { "uniref90_id": "UniRef90_D1J867", "gene": "secY", "product": "preprotein translocase subunit SecY", "uniref50_id": "UniRef50_A0A448ZXS5", "cog_id": "COG0201", "cog_category": "U", "go_ids": [ "GO:0015031", "GO:0016020", "GO:0043952" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J867", "UniRef:UniRef50_A0A448ZXS5", "COG:COG0201", "COG:U", "GO:0015031", "GO:0016020", "GO:0043952" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXS5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXS5" ], "product": "Protein translocase subunit SecY" } }, "id": "GLJLFKCOHC_620", "locus": "GCA013368035_00606" }, { "type": "cds", "sequence": "contig_1", "start": 353444, "stop": 353542, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAGAAAAACTAAGTTGGAAAAAGAGATTAGTCAAGAAAAGATCGAGCGCAAATTAGCACTAGATAAGTTTTTAGTTGATAAAAAAAACACTTGA", "aa": "MARKTKLEKEISQEKIERKLALDKFLVDKKNT", "aa_hexdigest": "52b053d98925f993c9317f2b09f4dc46", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3832.5150000000012, "isoelectric_point": 9.775808525085449 }, "id": "GLJLFKCOHC_621", "locus": "GCA013368035_00607" }, { "type": "cds", "sequence": "contig_1", "start": 353542, "stop": 353976, "strand": "-", "frame": 2, "gene": "rplO", "product": "50S ribosomal protein L15", "db_xrefs": [ "COG:COG0200", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "RefSeq:WP_012855556.1", "SO:0001217", "UniParc:UPI0001BC2246", "UniRef:UniRef100_D1J868", "UniRef:UniRef50_Q601J6", "UniRef:UniRef90_D1J868" ], "nt": "ATGCAATTACATAATTTAAAACCAACTCCAGGGGCAAGAAAAGACAAACACCGTGTTGGTCGTGGTCATGCTGCTGGAAAAGGAAAACAAGCCGGTAGAGGACAAAGTGGTCAAACAAAAAGAAGCAAAGTAAGACTAGGATTTGAAGGTGGCCAATTGCCACTATTTAGAAGAATTCCAAAACGTGGATTTAGCAATGTAAATCACGTTGAATTCCAAGTAGTAAATCTAGCAGATTTACAATCAAAATATCAAGATGGTGAAGTAGTTTCTTACGAAACACTTGCTGCAAGAAACTTGATTAAAGGAACATTACCAGTTAAAATTTTAGCTAAGGGTTCTTTAACTAAAAAACTTAATGTACAAATTCCAACACTTTCACAATCCGCTATTGAAGCTATTGAAAAAGTCGGAGGAAAAATAGAGGTTAAATAA", "aa": "MQLHNLKPTPGARKDKHRVGRGHAAGKGKQAGRGQSGQTKRSKVRLGFEGGQLPLFRRIPKRGFSNVNHVEFQVVNLADLQSKYQDGEVVSYETLAARNLIKGTLPVKILAKGSLTKKLNVQIPTLSQSAIEAIEKVGGKIEVK", "aa_hexdigest": "e48e82061d297ab366d048e8a4e1e848", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2246", "ncbi_nrp_id": "WP_012855556.1", "uniref100_id": "UniRef100_D1J868", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2246", "RefSeq:WP_012855556.1", "UniRef:UniRef100_D1J868" ] }, "ips": { "uniref100_id": "UniRef100_D1J868", "uniref90_id": "UniRef90_D1J868" }, "psc": { "uniref90_id": "UniRef90_D1J868", "gene": "rplO", "product": "50S ribosomal protein L15", "uniref50_id": "UniRef50_Q601J6", "cog_id": "COG0200", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_Q601J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q601J6" ], "product": "Large ribosomal subunit protein uL15" }, "genes": [ "rplO" ], "id": "GLJLFKCOHC_622", "locus": "GCA013368035_00608" }, { "type": "cds", "sequence": "contig_1", "start": 353986, "stop": 354654, "strand": "-", "frame": 2, "gene": "rpsE", "product": "30S ribosomal protein S5", "db_xrefs": [ "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02988", "RefSeq:WP_020002417.1", "SO:0001217", "UniParc:UPI000367CA6D", "UniRef:UniRef100_A0A454CA16", "UniRef:UniRef50_L0RWI9", "UniRef:UniRef90_D1J869" ], "nt": "ATGGCTGAAGTAGAAAAAAATATTAAAAATTCAGAAAAACCTGAATTAAAAGAAAATAAAACAGAAGGCACAAAGGTTGTTTCTTTTAAAAGAGAAAGATCAAATTCAAAAGATCTAAAATCAAGCGAAAATAAACCAAAACAATTCCAAAATCGTAATCAAGATCATTCAGCAAATCAAGCTCAAGCAAAACAATTTGAAGAAAAAGTTATTGACATTGCAAGAGTTACAACTGTTGTTAAGGGTGGACGTAGATTTTCATTCTCAGCTTATGTTGTTGTTGGTGACAAAAAAGGAAAAGTTGGATTTGGACACGGAAAAGCTAATGAAGTCCAAGATGCAATTAAAAAAGCAGTTAAAGATGCACAAAAAAATGTTTTCTCAGTTCCAATTTCAAACGGAACAATCCCTCATGAAATTCAAGATAAATTCTTAGCATCAAGAATCCAATTACGTCCAGCTCCAAAAGGTGCCGGAATCATTGCTTCAAACACCGTTCGTGCTGTTGTTGAATTAGCAGGTTACACCGACATTTCAACAAAAACTTATGGTTCAAGAACAAAACAAAACATTGTCCAAGCTACTATTAAGGCACTTAAAAAAGTTAGAACTGTTGAAGAAATCGCAAAATTACGCGATATTGATGTAAATCATCTATTATCAAAATAA", "aa": "MAEVEKNIKNSEKPELKENKTEGTKVVSFKRERSNSKDLKSSENKPKQFQNRNQDHSANQAQAKQFEEKVIDIARVTTVVKGGRRFSFSAYVVVGDKKGKVGFGHGKANEVQDAIKKAVKDAQKNVFSVPISNGTIPHEIQDKFLASRIQLRPAPKGAGIIASNTVRAVVELAGYTDISTKTYGSRTKQNIVQATIKALKKVRTVEEIAKLRDIDVNHLLSK", "aa_hexdigest": "bcbc4611471dda464e1e9604f871d769", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI000367CA6D", "ncbi_nrp_id": "WP_020002417.1", "uniref100_id": "UniRef100_A0A454CA16", "db_xrefs": [ "SO:0001217", "UniParc:UPI000367CA6D", "RefSeq:WP_020002417.1", "UniRef:UniRef100_A0A454CA16" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA16", "uniref90_id": "UniRef90_D1J869" }, "psc": { "uniref90_id": "UniRef90_D1J869", "gene": "rpsE", "product": "30S ribosomal protein S5", "uniref50_id": "UniRef50_L0RWI9", "kegg_orthology_id": "K02988", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_L0RWI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RWI9" ], "product": "Small ribosomal subunit protein uS5" }, "genes": [ "rpsE" ], "id": "GLJLFKCOHC_623", "locus": "GCA013368035_00609" }, { "type": "cds", "sequence": "contig_1", "start": 354654, "stop": 355001, "strand": "-", "frame": 3, "gene": "rplR", "product": "50S ribosomal protein L18", "db_xrefs": [ "COG:COG0256", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855558.1", "SO:0001217", "UniParc:UPI0001BC2248", "UniRef:UniRef100_D1J870", "UniRef:UniRef50_A0A6M4JHS6", "UniRef:UniRef90_D1J870" ], "nt": "ATGTTATCAAGAAATTTAAAACGTAAAGCAAAACACTTAAAGATTACTAACAAATTGTCAAAAGGAACTGCATTAGTTCCTAGAGTTGTTGTTTTTAAATCTTTACATGCATTTTATGCACAAGCAGTAAATGATGAATTGCACACCACAATTGCTTCTTCATCAACACAAATACTAGAAAATAAAGCAAACAATATTGATGCAGTTAAATTAGTTGCAAAAGATTTTGCTAAAAAATTATTGGCAATCAATGTAAAATCAATAGTGTTTGATCGTTCAGGATACATATACCACGGAAAATTAGCAGCTTTTGCTGATACTCTAAGAGAAGAAGGGATTAAATTCTAA", "aa": "MLSRNLKRKAKHLKITNKLSKGTALVPRVVVFKSLHAFYAQAVNDELHTTIASSSTQILENKANNIDAVKLVAKDFAKKLLAINVKSIVFDRSGYIYHGKLAAFADTLREEGIKF", "aa_hexdigest": "4512354e3b284c6d5db3f37ab085dddf", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2248", "ncbi_nrp_id": "WP_012855558.1", "uniref100_id": "UniRef100_D1J870", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2248", "RefSeq:WP_012855558.1", "UniRef:UniRef100_D1J870" ] }, "ips": { "uniref100_id": "UniRef100_D1J870", "uniref90_id": "UniRef90_D1J870" }, "psc": { "uniref90_id": "UniRef90_D1J870", "gene": "rplR", "product": "50S ribosomal protein L18", "uniref50_id": "UniRef50_A0A6M4JHS6", "cog_id": "COG0256", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6M4JHS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6M4JHS6" ], "product": "Large ribosomal subunit protein uL18" }, "genes": [ "rplR" ], "id": "GLJLFKCOHC_624", "locus": "GCA013368035_00610" }, { "type": "cds", "sequence": "contig_1", "start": 355023, "stop": 355562, "strand": "-", "frame": 3, "gene": "rplF", "product": "50S ribosomal protein L6", "db_xrefs": [ "COG:COG0097", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843", "RefSeq:WP_113256021.1", "SO:0001217", "UniParc:UPI000DDEB4E6", "UniRef:UniRef100_UPI000DDEB4E6", "UniRef:UniRef50_Q4A5D6", "UniRef:UniRef90_D1J871" ], "nt": "ATGTCTAGAATTGGAAAAAGAGTTTTAGAAATACCAAAAGATGTTGAAGTTAAATTAGATAAAAACCATATCGAAGTAAAAGGCAAATTAGGAGAATTGAGTTATACATTCTCACCATTAATTTCAGTAGTTATTGAAAACAACGAAATTAAGACATTGAGATCTAACGAAGAAAAATCTACAAAACAATTACACGGAACAACAAATGCAAACATTAAAAATATGTTAATAGGTGTTTCAAGTGGTTACAAAAAAGAAATTGAAATTAAAGGTGTAGGTTACAAAGCTACCTTAAAGGGAAATACAATTGAAATTATTGCTGGTTATTCACATGCTGTAAATTTAGAAGTTCCATCAAATCTAAAAATTGAATTACCAAAACCAACCAATATTATTATTTCAGGAATTGACAAACAAGCTGTTGGTGAATTTGCTGCAAATATTCGTGATGTAAGACGTCCAAGTCCATATTCTGGAAAAGGTATTATGTATAAAGATGAAGTTATTAGACGTAAGGAAGGAAAGACAGCTGCTAAATAG", "aa": "MSRIGKRVLEIPKDVEVKLDKNHIEVKGKLGELSYTFSPLISVVIENNEIKTLRSNEEKSTKQLHGTTNANIKNMLIGVSSGYKKEIEIKGVGYKATLKGNTIEIIAGYSHAVNLEVPSNLKIELPKPTNIIISGIDKQAVGEFAANIRDVRRPSPYSGKGIMYKDEVIRRKEGKTAAK", "aa_hexdigest": "c80c96213ea9b8a4bebf6ea44cf47627", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI000DDEB4E6", "ncbi_nrp_id": "WP_113256021.1", "uniref100_id": "UniRef100_UPI000DDEB4E6", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DDEB4E6", "RefSeq:WP_113256021.1", "UniRef:UniRef100_UPI000DDEB4E6" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DDEB4E6", "uniref90_id": "UniRef90_D1J871" }, "psc": { "uniref90_id": "UniRef90_D1J871", "gene": "rplF", "product": "50S ribosomal protein L6", "uniref50_id": "UniRef50_Q4A5D6", "cog_id": "COG0097", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412", "GO:0019843" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A5D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A5D6" ], "product": "Large ribosomal subunit protein uL6" }, "genes": [ "rplF" ], "id": "GLJLFKCOHC_625", "locus": "GCA013368035_00611" }, { "type": "cds", "sequence": "contig_1", "start": 355587, "stop": 355997, "strand": "-", "frame": 3, "gene": "rpsH", "product": "30S ribosomal protein S8", "db_xrefs": [ "COG:COG0096", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_Q6MSN9", "UniRef:UniRef90_D1J872" ], "nt": "ATGGCTATTATTATAGATCCAGTTGCAGATATGTTTGTACGTATTAAAAATGCAATTTCTCGTAAATATCATGAAGTTGTATTGCCACATTCAAACAAGAAAGCAAAAATTTTAGAAATTATTAAAAGAGAAGGATATATTGTTGATTTTGAAGTTGTAAAAAACGAAAAAACCAATTTTAAAGAAATAAAAGTTACTTTAAAATACAAAGGTATGAATCAAAATCAATCAGCAATTTCTGGTCTTAAAAAAGTTTCAAAACCTGGCTTAAAAGTATATTCATCTGCTGAAAAATTGCCTAGAGTTTTAAGTGGATATGGAACAGCTATTATTTCAACATCAAAAGGTTTATTAACAGATAAAGAAGCTCGTAAGGCTAATGTTGGTGGCGAAGTTATCGCCTTCATTTGA", "aa": "MAIIIDPVADMFVRIKNAISRKYHEVVLPHSNKKAKILEIIKREGYIVDFEVVKNEKTNFKEIKVTLKYKGMNQNQSAISGLKKVSKPGLKVYSSAEKLPRVLSGYGTAIISTSKGLLTDKEARKANVGGEVIAFI", "aa_hexdigest": "44621eacfa9ce74a148bdad8879e46d6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J872", "query_cov": 1.0, "subject_cov": 0.9927007299270073, "identity": 1.0, "score": 256.0, "evalue": 4.4e-86, "valid": true, "gene": "rpsH", "product": "30S ribosomal protein S8", "uniref50_id": "UniRef50_Q6MSN9", "cog_id": "COG0096", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6MSN9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6MSN9" ], "product": "Small ribosomal subunit protein uS8" }, "genes": [ "rpsH" ], "id": "GLJLFKCOHC_626", "locus": "GCA013368035_00612" }, { "type": "cds", "sequence": "contig_1", "start": 356003, "stop": 356185, "strand": "-", "frame": 1, "gene": null, "product": "Small ribosomal subunit protein uS14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "nt": "ATGGCAAGAACATCATTAAAGGTTAAAGCCGAACGCCAACCTAAATTTAAGGTTAGAAAATATACTCGTTGCCAACTTTGTGGCCGTGTTCACGCTGTATTGCGTAAATATAAAATTTGTAGAATTTGTTTTAGAAAATTAGCACATGAAGGAAAACTTCCTGGTGTTAAGAAAGCGAGCTGA", "aa": "MARTSLKVKAERQPKFKVRKYTRCQLCGRVHAVLRKYKICRICFRKLAHEGKLPGVKKAS", "aa_hexdigest": "4b14d9dfdd9d224ff7a908e546cd8f55", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A448ZVU2", "query_cov": 1.0, "subject_cov": 0.9836065573770492, "identity": 0.85, "score": 106.0, "evalue": 2.85e-29, "valid": false, "product": "type Z 30S ribosomal protein S14", "uniref50_id": "UniRef50_Q9PQP7", "kegg_orthology_id": "K02954", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PQP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PQP7" ], "product": "Small ribosomal subunit protein uS14" }, "genes": [], "id": "GLJLFKCOHC_627", "locus": "GCA013368035_00613" }, { "type": "cds", "sequence": "contig_1", "start": 356512, "stop": 356757, "strand": "-", "frame": 2, "gene": null, "product": "50S ribosomal protein L5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "nt": "ATGGCAAAACAAATTAAATTAGCATTAGAACAAAAATATTTAGACGAAATTCGTTTCGCGCTAGTTAAAGAATTTGGTTACACCAGTGTAATGCAAGCTCCTAAACTAGAAAAAATCGTTATTAACATGACTGCCGGTAACGAAGTTTCAAACTCAAAGGCAATCGAAGAAGTTATGGTTGAACTTGCTCAAATTACAGGTCAAAAACCATATCAAACAACCGCTAAGAAATCATTGGCTTCATGA", "aa": "MAKQIKLALEQKYLDEIRFALVKEFGYTSVMQAPKLEKIVINMTAGNEVSNSKAIEEVMVELAQITGQKPYQTTAKKSLAS", "aa_hexdigest": "7a5beec12a4a6cd8a099cdf0e8effdc6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1CF638", "query_cov": 0.8888888888888888, "subject_cov": 0.8571428571428571, "identity": 0.569, "score": 79.7, "evalue": 5.85e-18, "valid": false, "product": "50S ribosomal protein L5", "uniref50_id": "UniRef50_A0A2X3HCF5", "kegg_orthology_id": "K02931" }, "pscc": { "uniref50_id": "UniRef50_A0A2X3HCF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X3HCF5" ], "product": "50S ribosomal protein L5" }, "genes": [], "id": "GLJLFKCOHC_628", "locus": "GCA013368035_00614" }, { "type": "cds", "sequence": "contig_1", "start": 356835, "stop": 357107, "strand": "-", "frame": 3, "gene": "rplX", "product": "50S ribosomal protein L24", "db_xrefs": [ "COG:COG0198", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "SO:0001217", "UniRef:UniRef50_A0A448ZW26", "UniRef:UniRef90_A0A454CAH0" ], "nt": "ATGGCTCAAGCTAAAATTAGAAAAAATGACATGGTATTAATTATTGCTGGTAAAGACAAAGGAAAATTTGCTGCTGTTTTATCAACAAATCAAAAAGAACAAACTGTTGTTTTAAAAGGCGTAAACATTAAAACTAAACACCACAGACCATCACAAGAAAATAATGATGGAAAAATTGAAAAGAAAGAATACCCAATTCATATTTCAAACGTTGCTTATTTGGTTAAAAAAGGTGCTCAAGGTCAAAAATCAATCGGTTCAAAAATTGGTTGA", "aa": "MAQAKIRKNDMVLIIAGKDKGKFAAVLSTNQKEQTVVLKGVNIKTKHHRPSQENNDGKIEKKEYPIHISNVAYLVKKGAQGQKSIGSKIG", "aa_hexdigest": "a74a670b853a52000f9ce7c62c8ae22b", "start_type": "ATG", "rbs_motif": "GGAGG", "psc": { "uniref90_id": "UniRef90_A0A454CAH0", "query_cov": 1.0, "subject_cov": 0.8108108108108109, "identity": 0.9890000000000001, "score": 172.0, "evalue": 2.48e-54, "valid": true, "gene": "rplX", "product": "50S ribosomal protein L24", "uniref50_id": "UniRef50_A0A448ZW26", "cog_id": "COG0198", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW26" ], "product": "Large ribosomal subunit protein uL24" }, "genes": [ "rplX" ], "id": "GLJLFKCOHC_629", "locus": "GCA013368035_00615" }, { "type": "cds", "sequence": "contig_1", "start": 357109, "stop": 357477, "strand": "-", "frame": 2, "gene": "rplN", "product": "50S ribosomal protein L14", "db_xrefs": [ "COG:COG0093", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "KEGG:K02874", "RefSeq:WP_012855564.1", "SO:0001217", "UniParc:UPI0001BC229F", "UniRef:UniRef100_D1J876", "UniRef:UniRef50_A5FMZ0", "UniRef:UniRef90_D1J876" ], "nt": "ATGCTTAGTGAATTTTCAAGATGTAACGTTGCTGACAATTCAGGCGCAAAAGAAGTTATGATTATTAAAAACCTAGGTGGATCAATTGTTAGATCAACCAACATTGGTGATATTGTTGTTGTAACTATTAAAAAAGCTATTCCAAACGGAATTGTAAAAGAAGGCCAAGTTGTTAAAGCAGTTATAGTTAGAACAAAACGTGGTTTAATGCGTGAAAATGGTTCTCATATAAGATTTGATGACAATGCTGTTGTATTAATTAAGGAAGATAACTCATTAAGAGGAACTCGGGTATTTGGTCCAGTAGCAAGAGAATTACGTGAAAAAGGTTTTTTAAAGATTGTTTCATTAGCTCCGGAGGTTTTATAA", "aa": "MLSEFSRCNVADNSGAKEVMIIKNLGGSIVRSTNIGDIVVVTIKKAIPNGIVKEGQVVKAVIVRTKRGLMRENGSHIRFDDNAVVLIKEDNSLRGTRVFGPVARELREKGFLKIVSLAPEVL", "aa_hexdigest": "c87871ee0223ca5673069e09b0bae576", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC229F", "ncbi_nrp_id": "WP_012855564.1", "uniref100_id": "UniRef100_D1J876", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC229F", "RefSeq:WP_012855564.1", "UniRef:UniRef100_D1J876" ] }, "ips": { "uniref100_id": "UniRef100_D1J876", "uniref90_id": "UniRef90_D1J876" }, "psc": { "uniref90_id": "UniRef90_D1J876", "gene": "rplN", "product": "50S ribosomal protein L14", "uniref50_id": "UniRef50_A5FMZ0", "cog_id": "COG0093", "cog_category": "J", "kegg_orthology_id": "K02874", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934" ] }, "pscc": { "uniref50_id": "UniRef50_A5FMZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A5FMZ0" ], "product": "Large ribosomal subunit protein uL14" }, "genes": [ "rplN" ], "id": "GLJLFKCOHC_630", "locus": "GCA013368035_00616" }, { "type": "cds", "sequence": "contig_1", "start": 357470, "stop": 357754, "strand": "-", "frame": 1, "gene": "rpsQ", "product": "30S ribosomal protein S17", "db_xrefs": [ "COG:COG0186", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855565.1", "SO:0001217", "UniParc:UPI0001BC22A0", "UniRef:UniRef100_D1J877", "UniRef:UniRef50_Q98PZ1", "UniRef:UniRef90_D1J877" ], "nt": "ATGGAAACTACAGTTAGAGAAAATCGTCGTAAAACTTTAGTTGGAACAGTTGTATCAACTAAAAACCAAAAAACAATTACTGTTGTTGTAGAAATTTACGAAAAACACCCTCTTTATTCAAAACGTTTTAAAAAATCAAAGAAATTCGCAGTTCATGATGAACAAAATTTAGCTAAAGTTGGTGACATTGTTAAAATTCAAGAAACAAGACCTTTATCAAAAACCAAACACTTTAGACTAGTAGAAATCAAATCACACGCAATTGAAGGAGCAGAAAATGCTTAG", "aa": "METTVRENRRKTLVGTVVSTKNQKTITVVVEIYEKHPLYSKRFKKSKKFAVHDEQNLAKVGDIVKIQETRPLSKTKHFRLVEIKSHAIEGAENA", "aa_hexdigest": "ea20c26ef5b5db251866ea7f325bed04", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI0001BC22A0", "ncbi_nrp_id": "WP_012855565.1", "uniref100_id": "UniRef100_D1J877", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A0", "RefSeq:WP_012855565.1", "UniRef:UniRef100_D1J877" ] }, "ips": { "uniref100_id": "UniRef100_D1J877", "uniref90_id": "UniRef90_D1J877" }, "psc": { "uniref90_id": "UniRef90_D1J877", "gene": "rpsQ", "product": "30S ribosomal protein S17", "uniref50_id": "UniRef50_Q98PZ1", "cog_id": "COG0186", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PZ1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PZ1" ], "product": "Small ribosomal subunit protein uS17" }, "genes": [ "rpsQ" ], "id": "GLJLFKCOHC_631", "locus": "GCA013368035_00617" }, { "type": "cds", "sequence": "contig_1", "start": 357754, "stop": 357954, "strand": "-", "frame": 2, "gene": "rpmC", "product": "50S ribosomal protein L29", "db_xrefs": [ "COG:COG0255", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855566.1", "SO:0001217", "UniParc:UPI0001BC22A1", "UniRef:UniRef100_D1J878", "UniRef:UniRef50_A0A3B0PQ96", "UniRef:UniRef90_D1J878" ], "nt": "ATGACATTTGCAGAATTAAAAGAAAAATCACTTGACGAACTAAATAAGTTGGTTGCTGATTTAAGAGCAGAACTATTCTCATTACGTTTCAAAAATGCTACAAGACAATTAGACGAAACCCACAAAATCCAATTAGTTAAAAAAGATATCGCAAGAACTTTAACAGCAATTAAAGCAAAAACTTTAGAAGGGAATAAATAA", "aa": "MTFAELKEKSLDELNKLVADLRAELFSLRFKNATRQLDETHKIQLVKKDIARTLTAIKAKTLEGNK", "aa_hexdigest": "cb17ed1367064946a7cec20043621673", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A1", "ncbi_nrp_id": "WP_012855566.1", "uniref100_id": "UniRef100_D1J878", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A1", "RefSeq:WP_012855566.1", "UniRef:UniRef100_D1J878" ] }, "ips": { "uniref100_id": "UniRef100_D1J878", "uniref90_id": "UniRef90_D1J878" }, "psc": { "uniref90_id": "UniRef90_D1J878", "gene": "rpmC", "product": "50S ribosomal protein L29", "uniref50_id": "UniRef50_A0A3B0PQ96", "cog_id": "COG0255", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PQ96", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PQ96" ], "product": "Large ribosomal subunit protein uL29" }, "genes": [ "rpmC" ], "id": "GLJLFKCOHC_632", "locus": "GCA013368035_00618" }, { "type": "cds", "sequence": "contig_1", "start": 358128, "stop": 358235, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00252.24" ], "nt": "ATGGGACGTGAAGGAAAAGTTATTATTAAAATATTCCCTCACATGTCAAAAACTAGTAAACCTATTGGTGTTCGTATGGGATCTGGTAAGGGTAGCCCAGATCAATGA", "aa": "MGREGKVIIKIFPHMSKTSKPIGVRMGSGKGSPDQ", "aa_hexdigest": "06b42c6d46d5e2a7d1d7dd09ca724493", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00252.24", "name": "Ribosomal_L16", "length": 32, "aa_cov": 0.9142857142857143, "hmm_cov": 1.0, "evalue": 3.580140007057034e-10, "score": 35.33918762207031, "start": 3, "stop": 34 } ], "seq_stats": { "molecular_weight": 3755.4388000000017, "isoelectric_point": 10.564772605895993 }, "id": "GLJLFKCOHC_633", "locus": "GCA013368035_00619" }, { "type": "cds", "sequence": "contig_1", "start": 358284, "stop": 358409, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTCAACCAAAAAGAACTAAACATAGAAAAATGTTCCGTATCCGCCACGACAAAGTTGTTGCACACCGTAATAATACAGTTGCATTTGGCGAATTTGGTTTAAAATCAACTTCATCAGCATGA", "aa": "MLQPKRTKHRKMFRIRHDKVVAHRNNTVAFGEFGLKSTSSA", "aa_hexdigest": "7c21f6da5b3d095a3e3e708e02acb1c9", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4781.533299999998, "isoelectric_point": 11.849047279357908 }, "id": "GLJLFKCOHC_634", "locus": "GCA013368035_00620" }, { "type": "cds", "sequence": "contig_1", "start": 358504, "stop": 358764, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00189.25" ], "nt": "TTGCTAGCAGAAGGAATTGCAATTAAATTAGAAAATCGTGGTAGTTTTAGACTAGCACAAAAATTTGCAATTAAAGCAGCATTAAAAGCTGGCGCAAAAGGTATTAAAACTTCAGTAGCTGGACGTCTAAATGGCGTTGATATGGCTAGAACAGAAGGATATACCGAAGGTGAAATGAAATTACATACTTTAAGACAAGATGTTGATTATGCTGTAACAACAGCCTTAACAACATATGGTATCTTAGGAGTTAAAGTATGA", "aa": "MLAEGIAIKLENRGSFRLAQKFAIKAALKAGAKGIKTSVAGRLNGVDMARTEGYTEGEMKLHTLRQDVDYAVTTALTTYGILGVKV", "aa_hexdigest": "12fab75bd1d3b9296d32e0538593a4dd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00189.25", "name": "Ribosomal_S3_C", "length": 81, "aa_cov": 0.9418604651162791, "hmm_cov": 1.0, "evalue": 2.2021296929235322e-32, "score": 106.8982925415039, "start": 6, "stop": 86 } ], "seq_stats": { "molecular_weight": 9199.638499999995, "isoelectric_point": 9.752470970153805 }, "id": "GLJLFKCOHC_635", "locus": "GCA013368035_00621" }, { "type": "cds", "sequence": "contig_1", "start": 359118, "stop": 359507, "strand": "-", "frame": 3, "gene": "rplV", "product": "50S ribosomal protein L22", "db_xrefs": [ "COG:COG0091", "COG:J", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625", "KEGG:K02890", "RefSeq:WP_012855569.1", "SO:0001217", "UniParc:UPI0001BC22A4", "UniRef:UniRef100_Q8GM55", "UniRef:UniRef50_Q98PY6", "UniRef:UniRef90_Q8GM55" ], "nt": "ATGGCTAAAGAAATTTTACAAAATTCAGCACATGCATCAGTTCGCATGCAAAGAATTTCACCACGTAAAGCAAGATTAGTAGCAGATTTAATTAGATATAAATCAGCAACTCAAGCAATAGTTATTCTAAAACATACACACAAAAAAGCGTCAGAAATTATTCTAAAACTTCTAAATTCAGCAATTGCTAATGCAACAAATAATGCTGGACTTGATGCAACTAAACTTTATGTAACTACCATTTTAGTAAATGATGGACCTACACTAAAAAGATTCCAACCTCACTCACGTGGACGTGCTTATGCAATTTTAAAAAGAACAAGTCATTTCTTTATTGAATTAACAGAAATCAATAATGTTGAAGAAATCAACAAAAAAGGAGATAAATAA", "aa": "MAKEILQNSAHASVRMQRISPRKARLVADLIRYKSATQAIVILKHTHKKASEIILKLLNSAIANATNNAGLDATKLYVTTILVNDGPTLKRFQPHSRGRAYAILKRTSHFFIELTEINNVEEINKKGDK", "aa_hexdigest": "7a8b68311e501c927669bb50a65848bd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC22A4", "ncbi_nrp_id": "WP_012855569.1", "uniref100_id": "UniRef100_Q8GM55", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A4", "RefSeq:WP_012855569.1", "UniRef:UniRef100_Q8GM55" ] }, "ips": { "uniref100_id": "UniRef100_Q8GM55", "uniref90_id": "UniRef90_Q8GM55" }, "psc": { "uniref90_id": "UniRef90_Q8GM55", "gene": "rplV", "product": "50S ribosomal protein L22", "uniref50_id": "UniRef50_Q98PY6", "cog_id": "COG0091", "cog_category": "J", "kegg_orthology_id": "K02890", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015934", "GO:0019843", "GO:0022625" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY6" ], "product": "Large ribosomal subunit protein uL22" }, "genes": [ "rplV" ], "id": "GLJLFKCOHC_636", "locus": "GCA013368035_00622" }, { "type": "cds", "sequence": "contig_1", "start": 359682, "stop": 359786, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTCGTTCATTAAAGAAAGCTCCCTTTGTTGATGATCATTTAATGAAGAAAGTTTTGGCTATTATTGAAAATAAATCACCAAAACGTCCAATTAAAACATGA", "aa": "MARSLKKAPFVDDHLMKKVLAIIENKSPKRPIKT", "aa_hexdigest": "5da938756da5c11a053df77e46912a7d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3904.734700000001, "isoelectric_point": 10.383809852600098 }, "id": "GLJLFKCOHC_637", "locus": "GCA013368035_00623" }, { "type": "cds", "sequence": "contig_1", "start": 359873, "stop": 360631, "strand": "-", "frame": 1, "gene": null, "product": "Large ribosomal subunit protein uL2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "nt": "ATGGCTATTAAAAAATTTAAAGCATATACCAATGGCCGTCGTAATATGTCTTCTCTTGATTATCAAGCAAATTTAACAGGACATGCTCCTGAAAAATCATTGCTTGTTGCTCTTCCAACAAAAGCAGGAAGAAACAATCAAGGTAAAATCACTACCCGTCATCAAGGTGGTAGATTGAAAAGATTCTATCGTATCATTGATTTTAAACGTCAAAAAGATGGAATCCCAGCTACTGTAAAAACTATTGAATATGACCCTAATCGTTCAGCTAATATTTCATTAGTTGTTTATAGAGATGGTGAAAAAAGATATATTTTAAGCCCTAAAGGTATTAAAATTGGACAAGTTATTTTGTCAGGTGATAATGTTGACATTCAAGTTGGTAACCATTTACCATTAGCCAACATTCCTGAAGGTACATTTGTTCACAATATTGAATTACAACCAAGACAAGGTGGAATCATTGCTAGATCAGCTGGATCATCAGCACAAATCTTAGGTAAAGATGAATCAGGTCGTTATGTAATTCTAAGATTAAAATCAGGCGAAGTTAGAAAAGTTTTAGCAATTAATCGTGCAACAGTTGGTGAAGTAGGAAACGAAGAACACTCACTTGTTAATATTGGTAAGGCAGGACGTAACCGTTTAAGAGGAATTAGACCAACAGTTCGTGGTTCAGCTATGAACCCTAATGATCACCCACATGGTGGTGGTGAAGGTAGACAACCTATTGGTAGAAAGAGTCCTATGACTCCATGA", "aa": "MAIKKFKAYTNGRRNMSSLDYQANLTGHAPEKSLLVALPTKAGRNNQGKITTRHQGGRLKRFYRIIDFKRQKDGIPATVKTIEYDPNRSANISLVVYRDGEKRYILSPKGIKIGQVILSGDNVDIQVGNHLPLANIPEGTFVHNIELQPRQGGIIARSAGSSAQILGKDESGRYVILRLKSGEVRKVLAINRATVGEVGNEEHSLVNIGKAGRNRLRGIRPTVRGSAMNPNDHPHGGGEGRQPIGRKSPMTP", "aa_hexdigest": "2d1053e9b4b069d6e507646decf318a5", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A0A3M0A1P8", "query_cov": 1.0, "subject_cov": 0.896797153024911, "identity": 0.897, "score": 457.0, "evalue": 1.04e-161, "valid": false, "gene": "rplB", "product": "50S ribosomal protein L2", "uniref50_id": "UniRef50_Q9AMK8", "kegg_orthology_id": "K02886", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0006412", "GO:0015934", "GO:0016740" ] }, "pscc": { "uniref50_id": "UniRef50_Q9AMK8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9AMK8" ], "product": "Large ribosomal subunit protein uL2" }, "genes": [], "id": "GLJLFKCOHC_638", "locus": "GCA013368035_00624" }, { "type": "cds", "sequence": "contig_1", "start": 360725, "stop": 361156, "strand": "-", "frame": 1, "gene": "rplW", "product": "50S ribosomal protein L23", "db_xrefs": [ "COG:COG0089", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855572.1", "SO:0001217", "UniParc:UPI00000BF6FA", "UniRef:UniRef100_D1J884", "UniRef:UniRef50_Q98PY3", "UniRef:UniRef90_D1J884" ], "nt": "ATGAATATAAATGAAGTTATTAAATACCCACTTTTAACAGAAAAAAGTGAATTATCACGTGAAAATAAAAACGTTTATACATTTGTAGTTGATAAAAGAACAAACAAAATCGAAGTTAAAAAAGCAGTTGAATTTATTTTTGATGTAAAAGTATTAAAAGTTAACATTACTAATTACGATAAAAAACCTGCTAAATTAGGACGTTTCTCAGGTTTTAAAAATTCAGTTAAAAAAGCAACTGTATATTTAGCAAAAGATTCAAAAATTATTCTATTTGCTGAAGAATTAAAGAAAGCTAACAAAGAAGCTGAAAAAGCAACAGCAGAAAAAGAAGTTAAAGAAGAAAAACCTGCTGAACCTACCGCTGCAGAATTAAAAGCAGCAGAAAAAATTAAAGCAGCTGAAGCTAAAAAAGCTGCTGAAAACAAATAG", "aa": "MNINEVIKYPLLTEKSELSRENKNVYTFVVDKRTNKIEVKKAVEFIFDVKVLKVNITNYDKKPAKLGRFSGFKNSVKKATVYLAKDSKIILFAEELKKANKEAEKATAEKEVKEEKPAEPTAAELKAAEKIKAAEAKKAAENK", "aa_hexdigest": "e9516e60a03d444412cf50f35f7ebe06", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00000BF6FA", "ncbi_nrp_id": "WP_012855572.1", "uniref100_id": "UniRef100_D1J884", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BF6FA", "RefSeq:WP_012855572.1", "UniRef:UniRef100_D1J884" ] }, "ips": { "uniref100_id": "UniRef100_D1J884", "uniref90_id": "UniRef90_D1J884" }, "psc": { "uniref90_id": "UniRef90_D1J884", "gene": "rplW", "product": "50S ribosomal protein L23", "uniref50_id": "UniRef50_Q98PY3", "cog_id": "COG0089", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY3" ], "product": "Large ribosomal subunit protein uL23" }, "genes": [ "rplW" ], "id": "GLJLFKCOHC_639", "locus": "GCA013368035_00625" }, { "type": "cds", "sequence": "contig_1", "start": 361156, "stop": 361293, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTCTTATCAGCAAGAAACTTACCAAATGTAACTGTAACAAAAGTAACTAGTTTATCAGTTGAATCTTTATTGGCAGCAGATGTTTTAGTTATTAGTTCAGATGATGTTAAATACTTAGAAGGATTGGTTAAATAA", "aa": "MFLSARNLPNVTVTKVTSLSVESLLAADVLVISSDDVKYLEGLVK", "aa_hexdigest": "8430c512fd0c8253469c444377393a46", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.5984, "isoelectric_point": 4.8610639572143555 }, "id": "GLJLFKCOHC_640", "locus": "GCA013368035_00626" }, { "type": "cds", "sequence": "contig_1", "start": 361939, "stop": 362124, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514", "UniRef:UniRef90_UPI001314D514" ], "nt": "ATGGCAACAAAAAATGTAACATTAGAAAAATTCTACATTTCTGAAAAATTTGATGAAAATCGTAATATTTCATTTAATTTAAAAAAGGCTAACCAAAAATCATTCAAGAATTTTGAAAATTTTTCAGAAGCATTAACAGAATTTATTGAACTTTCTGAAAAATCAAAAAACAATACTAGAGTATGA", "aa": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRV", "aa_hexdigest": "65cd46d9cd54cbf3847c176a252340c8", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 361827, "stop": 362124, "observations": { "insertion": [ 361860 ], "mutation": [ 361941 ], "stop": [ 361941 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001314D514", "query_cov": 1.0, "subject_cov": 0.6224489795918368, "identity": 1.0, "score": 115.0, "evalue": 3.69e-32, "gene_start": 1, "gene_end": 61, "reference_start": 1, "reference_end": 61, "reference_sequence": "MATKNVTLEKFYISEKFDENRNISFNLKKANQKSFKNFENFSEALTEFIELSEKSKNNTRVWFHRDGAYRGSVGLEKAKLILEKVTGEKLKMKKQSTT" }, "paralog": false, "identity": 0.9897959183673469, "score": 184.0, "evalue": 5.03e-62, "description": "Internal stop codon at 361941. Frameshift due to insertion around 361860. Nonsense mutation around 361941.", "psc": { "uniref90_id": "UniRef90_UPI001314D514", "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001314D514", "UniRef:UniRef50_UPI001314D514" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001314D514", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001314D514" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7242.053499999998, "isoelectric_point": 9.04905643463135 }, "id": "GLJLFKCOHC_641", "locus": "GCA013368035_00627" }, { "type": "cds", "sequence": "contig_1", "start": 362124, "stop": 362450, "strand": "-", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGCCAGGACATTTAGGAGCTGAACAAGTAACTGTTCAAAACCTAGAAGTTGTTAAAGAAGATGCAGAAAACAATATTTTATTGATCAAGGGATCAGTTCCTGGTGCTAAAGGAGCATTCTTAATCATTAAAACTGCTAAGAAATCAATTAAGAATAAACCAGTTATTAAATTATTGAATCTAAAAGAAGCTTTGGCTAAAAATGAAATCTTTACTCAAGCTAAAAAATACAATCTTGAATTAAACATGGATATGTCATTAAAAGAAATGAAAGCTGCTTTGGATGAAGCAATTGCTAAGGCCGGAGAAGAAGGAGAAAATAAATAA", "aa": "MPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK", "aa_hexdigest": "8579bc1a82bf19d1b9aee04b89886a80", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "rplC" ], "truncated": "3-prime", "pseudogene": { "start": 362124, "stop": 362750, "observations": { "mutation": [ 362465, 362564 ], "stop": [ 362465, 362564 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.40298507462686567, "identity": 1.0, "score": 201.0, "evalue": 3.99e-63, "gene_start": 1, "gene_end": 108, "reference_start": 161, "reference_end": 268, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9903846153846154, "score": 400.0, "evalue": 7.78e-144, "description": "Internal stop codon at 362465, 362564. Nonsense mutation around 362465, 362564.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "GLJLFKCOHC_642", "locus": "GCA013368035_00628" }, { "type": "cds", "sequence": "contig_1", "start": 362562, "stop": 362930, "strand": "-", "frame": 3, "gene": "rplC", "product": "50S ribosomal protein L3", "db_xrefs": [ "COG:COG0087", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "KEGG:K02906", "SO:0001217", "UniRef:UniRef50_Q98PY0", "UniRef:UniRef90_D1J886" ], "nt": "ATGAAAGGAATCTTAGGAAGAAAAGTTGGGATGACTCAACTATTTACTTCAGAAGGTAAATTAATACCTGTGACAGTAGTAGAAGTTAAACCAAATGTTGTTACTAAGGTTTTAACATCAGAAAAAAATGGTTATGTTGCTCTTCAATTAGCAACAGAAGATAAAAAATCTTCAAGAATCATTAAACCTGAAGTTAATTACTTTAAACAAGCAAATACAACACCTAAGCGCTTCGTAAAAGAAATCCGTGATATGTCAGGATTTTCATTAGGTGATACTGTAGATGCTTCATTATTTACCGCTGGAGAAGTTGTTGATGTAACAGCTATTTCTAAAGGTAAAGGATTTGCGGGTACCATCAAGAGATGA", "aa": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKR", "aa_hexdigest": "565ff623fc584ade78acfc33a3752bd4", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "rplC" ], "truncated": "5-prime", "pseudogene": { "start": 362259, "stop": 362930, "observations": { "mutation": [ 362465, 362564 ], "stop": [ 362465, 362564 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J886", "query_cov": 1.0, "subject_cov": 0.4552238805970149, "identity": 1.0, "score": 233.0, "evalue": 1.81e-75, "gene_start": 1, "gene_end": 122, "reference_start": 1, "reference_end": 122, "reference_sequence": "MKGILGRKVGMTQLFTSEGKLIPVTVVEVKPNVVTKVLTSEKNGYVALQLATEDKKSSRIIKPEVNYFKQANTTPKRFVKEIRDMSGFSLGDTVDASLFTAGEVVDVTAISKGKGFAGTIKRWNQHIGPKSHGGGGGSQPVRQTGSLGDISGNRVWKGMTMPGHLGAEQVTVQNLEVVKEDAENNILLIKGSVPGAKGAFLIIKTAKKSIKNKPVIKLLNLKEALAKNEIFTQAKKYNLELNMDMSLKEMKAALDEAIAKAGEEGENK" }, "paralog": false, "identity": 0.9910313901345291, "score": 427.0, "evalue": 2.1e-154, "description": "Internal stop codon at 362465, 362564. Nonsense mutation around 362465, 362564.", "psc": { "uniref90_id": "UniRef90_D1J886", "gene": "rplC", "product": "50S ribosomal protein L3", "uniref50_id": "UniRef50_Q98PY0", "cog_id": "COG0087", "cog_category": "J", "kegg_orthology_id": "K02906", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J886", "UniRef:UniRef50_Q98PY0", "COG:COG0087", "COG:J", "KEGG:K02906", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PY0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PY0" ], "product": "Large ribosomal subunit protein uL3" } }, "id": "GLJLFKCOHC_643", "locus": "GCA013368035_00629" }, { "type": "cds", "sequence": "contig_1", "start": 363000, "stop": 363308, "strand": "-", "frame": 3, "gene": "rpsJ", "product": "30S ribosomal protein S10", "db_xrefs": [ "COG:COG0051", "COG:J", "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855575.1", "SO:0001217", "UniParc:UPI0001BC22A6", "UniRef:UniRef100_D1J887", "UniRef:UniRef50_D1J887", "UniRef:UniRef90_D1J887" ], "nt": "ATGAAACTAGAAGTCAAAATTAAATCTTTTGATGCCCACTTAGTAGATGAAGCTGCTAAAAAAATAGTTTTATTAGCTCAAGCTGAAAAAGCAACTATTAGTGGTCCAATTCCTTTACCAACACATAAAGAAATTATTACCATCTTAAGATCTGTTCACGTTAACAAGAAATCTCGTGAACAATTTGAAAGCAGAACTCATAAACGTTTAATCGTTTTAGATAAAGTTTCTGAATCATTAGTTGATAAGGCTAAGAGATTAGATTTATCTGCTGGTGTACAAATAAGTTTAAAAACTGTTAATCAATAA", "aa": "MKLEVKIKSFDAHLVDEAAKKIVLLAQAEKATISGPIPLPTHKEIITILRSVHVNKKSREQFESRTHKRLIVLDKVSESLVDKAKRLDLSAGVQISLKTVNQ", "aa_hexdigest": "f6c7d2f92638f7c729b3f6b0f0f4476d", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A6", "ncbi_nrp_id": "WP_012855575.1", "uniref100_id": "UniRef100_D1J887", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A6", "RefSeq:WP_012855575.1", "UniRef:UniRef100_D1J887" ] }, "ips": { "uniref100_id": "UniRef100_D1J887", "uniref90_id": "UniRef90_D1J887" }, "psc": { "uniref90_id": "UniRef90_D1J887", "gene": "rpsJ", "product": "30S ribosomal protein S10", "uniref50_id": "UniRef50_D1J887", "cog_id": "COG0051", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_D1J887", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J887" ], "product": "Small ribosomal subunit protein uS10" }, "genes": [ "rpsJ" ], "id": "GLJLFKCOHC_644", "locus": "GCA013368035_00630" }, { "type": "cds", "sequence": "contig_1", "start": 365852, "stop": 365959, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTGTATTTATATACAAATTCTTTGTCCAGATATGATTAAAAACAATTTAATGGGTAAAGAATCGGAAGGATATATTTACTTAGATCTTTCGGATGCTGATTAA", "aa": "MPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDAD", "aa_hexdigest": "9511f5b3800971f3e26ddc174191068a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4010.6492000000017, "isoelectric_point": 4.1011266708374015 }, "id": "GLJLFKCOHC_645", "locus": "GCA013368035_00631" }, { "type": "cds", "sequence": "contig_1", "start": 366008, "stop": 366652, "strand": "-", "frame": 1, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J888", "UniRef:UniRef90_D1J888" ], "nt": "ATGAAAAAAAATATTTTATTAAGTTTTATTTTTCCAGCTGTGTCATTATTACCTCTTTTAAGCATATCTTGCAAGGAAACTACCACAATTAATAATACCTTAGATACAAGTAATAACACTATTGATATAGATAATAGTGGCACTACAGATACTAATGATAAAAATACAAATAGCACAAATACTGGTACTACTGATACTGATAATAAGAATATAGGTAATTCTAGTGTTGGCACTACAGATACTAATGACAAAAATACAGATAGCACAAATACCGGTACCGCCGATACTAACGACAAGAAAGATGACAATAAAGATACAAATAACGAAAATACTGATGATACAGATCCCGTACAACCCGATAGACTTCATAGAATGTTTCAAAGAAAGCTATTATCCGCTTCACAAATTTCAGAAATAACAACACAATTTTCATTTGAACTTTCTAAAGAGGGTAAAAAATTTGTAGCCAATAATGGAGCTAAAGCCTTAATTGAAATAGTTAATAAAGCAATTGATGAAACTAAAAAATACGACACTATTGAAATGTTAAAAAGTCAAGAAAGAATTGCTTATACATATTTTATGGAAGCTAATGACATTAAAAAATATTTTGATATAAAAATTCCTGAAAATCTTATGCCTTGA", "aa": "MKKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTSNNTIDIDNSGTTDTNDKNTNSTNTGTTDTDNKNIGNSSVGTTDTNDKNTDSTNTGTADTNDKKDDNKDTNNENTDDTDPVQPDRLHRMFQRKLLSASQISEITTQFSFELSKEGKKFVANNGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMP", "aa_hexdigest": "2ca4cb08828415ecfd2ed6d1b9fa9047", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 365852, "stop": 366646, "observations": { "mutation": [ 366010 ], "stop": [ 366010 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J888", "query_cov": 0.9906542056074766, "subject_cov": 0.7881040892193308, "identity": 0.943, "score": 246.0, "evalue": 3.46e-79, "gene_start": 3, "gene_end": 214, "reference_start": 6, "reference_end": 217, "reference_sequence": "MKGIWKNILLSFIFPAVSLLPLLSISCKETTTINNTLDTNNNTIDIDNSGTTDTNDKNTNSTNTGTTDTDNKNIGNSSVDTTDTNDKNTDSTNTGTTDTSDKKDDNKDTNNENTDDTDPEQPNRLHRIFQRKLLSASQISEITTQFSFELSKEGKKFVANKGAKALIEIVNKAIDETKKYDTIEMLKSQERIAYTYFMEANDIKKYFDIKIPENLMPWGHKLLIEFLKKGEEKNMPCIYIQILCPDMIKNNLMGKESEGYIYLDLSDVD" }, "paralog": false, "identity": 0.946969696969697, "score": 358.0, "evalue": 6.97e-126, "description": "Internal stop codon at 366010. Nonsense mutation around 366010.", "psc": { "uniref90_id": "UniRef90_D1J888", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J888", "UniRef:UniRef50_D1J888" ] }, "pscc": { "uniref50_id": "UniRef50_D1J888", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J888" ], "product": "Variable surface lipoprotein" } }, "id": "GLJLFKCOHC_646", "locus": "GCA013368035_00632" }, { "type": "cds", "sequence": "contig_1", "start": 367085, "stop": 367402, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTTATATTGAAGGTCCCGGACACGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCTGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCACCTGAAACACCTGGTTCAATTCACGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGCGAAGCAGAAACAGGCCCACTAGCAACTAGCTGA", "aa": "MFYIEGPGHGGQVMISNSYLDGSYSEIYPEISQDEAGLAKMFKRFSFPGGTASHAAPETPGSIHEGGELGYSISHGTGAILDNPDVICAAVVGDGEAETGPLATS", "aa_hexdigest": "cb9a70a1207895298182aa6f7a6c75c0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10793.721599999992, "isoelectric_point": 4.3166601181030275 }, "id": "GLJLFKCOHC_647", "locus": "GCA013368035_00633" }, { "type": "cds", "sequence": "contig_1", "start": 367273, "stop": 367593, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAGATTACTTTTATCTTCGCTTCATTCAACGAAAATTGGATTTCAGCCTAAACCTTCGAAGTATTTTTTAATTTCTTCTTTTGGTTTTCTTGAAAGAAGTGTAGGGTTTGAAATTTTTCCACCATTCAAATGTAATATAGGAAGTATTACCCCATCATTTACAGGGTTAATAAAGGCATTTGAGAATCAGCTAGTTGCTAGTGGGCCTGTTTCTGCTTCGCCATCGCCAACAACAGCTGCACAAATAACATCAGGGTTATCTAATATTGCTCCTGTTCCATGGCTTATAGAATATCCTAATTCACCACCTTCGTGA", "aa": "MSRLLLSSLHSTKIGFQPKPSKYFLISSFGFLERSVGFEIFPPFKCNIGSITPSFTGLIKAFENQLVASGPVSASPSPTTAAQITSGLSNIAPVPWLIEYPNSPPS", "aa_hexdigest": "ed35c6878cefe3d211cde929e3b5d564", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11345.958199999985, "isoelectric_point": 9.359794044494631 }, "id": "GLJLFKCOHC_648", "locus": "GCA013368035_00634" }, { "type": "cds", "sequence": "contig_1", "start": 367604, "stop": 367699, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAAATCATTAGATAAAGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCTGCTGAAGAAGCAACAAGACCAACTTGA", "aa": "MAKSLDKAIESIKEIQAEARKKPAEEATRPT", "aa_hexdigest": "9dabdcbb4dcd8d8b3e81f6139aafeee5", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3440.9214000000006, "isoelectric_point": 8.141083717346195 }, "id": "GLJLFKCOHC_649", "locus": "GCA013368035_00635" }, { "type": "cds", "sequence": "contig_1", "start": 368051, "stop": 368179, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGAAATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGA", "aa": "MAEMAKFAADIMKDNPSSFRVFGPDETKSNRMFALFNVTNRQ", "aa_hexdigest": "7538684bd81affb55bcf87471adf19fe", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4814.4602, "isoelectric_point": 8.253452110290528 }, "id": "GLJLFKCOHC_650", "locus": "GCA013368035_00636" }, { "type": "cds", "sequence": "contig_1", "start": 368567, "stop": 368695, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAGCCTTAACTGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGTTGAAGATGCTGAAGAATTACTAGAAAAAGGTTATAAAGTTGTTCCTTGA", "aa": "MNKALTERNVINLIVASKQPREQFFTVEDAEELLEKGYKVVP", "aa_hexdigest": "06265444dd5f8e3c2b7c8e8889e02782", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4848.5314, "isoelectric_point": 5.189195442199705 }, "id": "GLJLFKCOHC_651", "locus": "GCA013368035_00637" }, { "type": "cds", "sequence": "contig_1", "start": 368771, "stop": 369211, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTTTATGTTCTTGACTTATTAAAATTGAGATCTAGAAAAATAGACCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGATTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGCGATATTACAACATCGTTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAACTAGACCAAAAACTAGAATATCATAGAAATTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGA", "aa": "MAAISLINQEYPHLKIRYVYVLDLLKLRSRKIDPRGISDEEFDKVFTKNKPIIFAFHGFEGLLRDIFFTRSNHNLIAHGYRENGDITTSFDIRQLSEMDRYHIAKDAAEAVYGKDAKAFMNKLDQKLEYHRNYIDEYGYDMPEVVE", "aa_hexdigest": "59f58ddc36c55ba5c33ce12997ad2aed", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0AA37XNB4", "query_cov": 0.9863013698630136, "subject_cov": 0.8674698795180723, "identity": 0.59, "score": 189.0, "evalue": 3.88e-59, "valid": false, "product": "Xylulose 5-phosphate/Fructose 6-phosphate phosphoketolase C-terminal domain-containing protein", "uniref50_id": "UniRef50_A0A3B0NYC0" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17242.383899999986, "isoelectric_point": 6.071961784362792 }, "id": "GLJLFKCOHC_652", "locus": "GCA013368035_00638" }, { "type": "cds", "sequence": "contig_1", "start": 369318, "stop": 369533, "strand": "+", "frame": 3, "gene": "rpmE", "product": "50S ribosomal protein L31", "db_xrefs": [ "COG:COG0254", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_012855578.1", "SO:0001217", "UniParc:UPI0001BC22A9", "UniRef:UniRef100_D1J890", "UniRef:UniRef50_Q6KIG2", "UniRef:UniRef90_D1J890" ], "nt": "ATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAA", "aa": "MQKDIHPKYNQIKAVCSSCNSTIEFGSTADKVTLDVCSNCHAFYTGNRTDVKARGRVERFNKILEKSHKKN", "aa_hexdigest": "14ec0f3da0d8b604325a5ed87f9f7f2a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC22A9", "ncbi_nrp_id": "WP_012855578.1", "uniref100_id": "UniRef100_D1J890", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC22A9", "RefSeq:WP_012855578.1", "UniRef:UniRef100_D1J890" ] }, "ips": { "uniref100_id": "UniRef100_D1J890", "uniref90_id": "UniRef90_D1J890" }, "psc": { "uniref90_id": "UniRef90_D1J890", "gene": "rpmE", "product": "50S ribosomal protein L31", "uniref50_id": "UniRef50_Q6KIG2", "cog_id": "COG0254", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG2" ], "product": "Large ribosomal subunit protein bL31" }, "genes": [ "rpmE" ], "id": "GLJLFKCOHC_653", "locus": "GCA013368035_00639" }, { "type": "cds", "sequence": "contig_1", "start": 369629, "stop": 369826, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891", "UniRef:UniRef90_D1J891" ], "nt": "ATGAATATAAATTTCAAACTAATGAGCGAAGAAGAAACAAAAAACATTGTAGGAGGTTTTGCAATTACTGCTTTAATTTCAACTATTTTGTCTGTAGTAACAAGCACTGTTGGAATTATTGGAACATCAATAGGCTTAGCTAAGACTTTAAATGCAAATAAAGGAGAAGTAAAAGCTGGAAATATTTCATTGAAATGA", "aa": "MNINFKLMSEEETKNIVGGFAITALISTILSVVTSTVGIIGTSIGLAKTLNANKGEVKAGNISLK", "aa_hexdigest": "1bc3aa36b3505ccc9a4d5d1491901159", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "both", "pseudogene": { "start": 369575, "stop": 369869, "observations": { "insertion": [ 369830 ], "stop": [ 369631 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J891", "query_cov": 1.0, "subject_cov": 0.6701030927835051, "identity": 0.985, "score": 115.0, "evalue": 4.25e-32, "gene_start": 1, "gene_end": 65, "reference_start": 15, "reference_end": 79, "reference_sequence": "MEYLLYLKLKGEIKMNINFKLMSEEETKNIVGGFAITALISTILSVVTSTVGIIGTSIGLAKTLNANKGEVKAGNISMKWDNSNENIGYAVGMHYCT" }, "paralog": false, "identity": 0.979381443298969, "score": 180.0, "evalue": 2.57e-60, "description": "Internal stop codon at 369631. Frameshift due to insertion around 369830.", "psc": { "uniref90_id": "UniRef90_D1J891", "uniref50_id": "UniRef50_D1J891", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J891", "UniRef:UniRef50_D1J891" ] }, "pscc": { "uniref50_id": "UniRef50_D1J891", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J891" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6722.819, "isoelectric_point": 9.303642082214353 }, "id": "GLJLFKCOHC_654", "locus": "GCA013368035_00640" }, { "type": "cds", "sequence": "contig_1", "start": 371277, "stop": 371693, "strand": "-", "frame": 3, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4", "UniRef:UniRef90_UPI002FE0DEE4" ], "nt": "ATGCTTGGTGGAATTAGTGACTTTGATTTTAAAGAAATTACGCCAGCTTCAATTCATGCTTTAATGTTAGAAAAATTGCATAATCTAGAAGCAAATATATTATTGAATTACGAACAATATAGATTTGTTAATGTTATTAAAGATATTAATAATTTTGTTATTGATTTAAGTAGTTACTATATCTCTATAACCAAAGATATTTTATATCTTGAAAAACAAGATGACCCTGAACGTAGAATGGTTCAATATGTTTTTAGTAACATTATTGAGGTTTTATTAAAAGCTCTTGCCCCAATATTGCCAACTACAACCGAAGAAATTTATAAATATTACAATAAACCTAACAAGTTGCCATCCGTTCATTTATTGCCATTTTTAGAAAGAAAAGATTTTGATTTTAAATTACAAGATCAATGA", "aa": "MLGGISDFDFKEITPASIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLPFLERKDFDFKLQDQ", "aa_hexdigest": "f0e0c8f51c491821a5f96690cb4abdcf", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 371169, "stop": 371684, "observations": { "mutation": [ 371189, 371279 ], "stop": [ 371189, 371279 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "query_cov": 0.9782608695652174, "subject_cov": 0.7627118644067796, "identity": 0.978, "score": 263.0, "evalue": 1.65e-88, "gene_start": 4, "gene_end": 138, "reference_start": 3, "reference_end": 137, "reference_sequence": "MLGISDFDFKEITPTSIHALMLEKLHNLEANILLNYEQYRFVNVIKDINNFVIDLSSYYISITKDILYLEKQDDPERRMVQYVFSNIIEVLLKALAPILPTTTEEIYKYYNKPNKLPSVHLLSFLERKDFDFKFQDQWKEFFEIKDEVYKLIEEKIKSQAIKRPNEAWVVIKAIQTL" }, "paralog": false, "identity": 0.9649122807017544, "score": 324.0, "evalue": 6.04e-115, "description": "Internal stop codon at 371189, 371279. Nonsense mutation around 371189, 371279.", "psc": { "uniref90_id": "UniRef90_UPI002FE0DEE4", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002FE0DEE4", "UniRef:UniRef50_UPI002FE0DEE4" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002FE0DEE4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002FE0DEE4" ], "product": "class I tRNA ligase family protein" } }, "id": "GLJLFKCOHC_655", "locus": "GCA013368035_00641" }, { "type": "cds", "sequence": "contig_1", "start": 371793, "stop": 371912, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBK0", "UniRef:UniRef90_A0A432FBK0" ], "nt": "TTGGTTTCTCATGGGTTTGTTTTAGATGGCAAAGGTCAAAAAATGTCAAAATCAAAAGGCAATGTTGTTGATCCTTTGGCTGTGATTGATAAATATGGAGCAGATATTTTAAGACTTTGA", "aa": "MVSHGFVLDGKGQKMSKSKGNVVDPLAVIDKYGADILRL", "aa_hexdigest": "3e3c4202b6a38a44c5865b9dd4e3e816", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 371793, "stop": 371954, "observations": { "mutation": [ 371939 ], "stop": [ 371939 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A432FBK0", "query_cov": 1.0, "subject_cov": 0.7358490566037735, "identity": 0.846, "score": 70.5, "evalue": 2.13e-15, "gene_start": 1, "gene_end": 39, "reference_start": 15, "reference_end": 53, "reference_sequence": "MNSIAYRGKSPYKNLVSHGFVLDGKNQKMSKSKGNVVDPLKIISKQGADILRL" }, "paralog": false, "identity": 0.7735849056603774, "score": 83.6, "evalue": 1.73e-23, "description": "Internal stop codon at 371939. Nonsense mutation around 371939.", "psc": { "uniref90_id": "UniRef90_A0A432FBK0", "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "uniref50_id": "UniRef50_A0A432FBK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A432FBK0", "UniRef:UniRef50_A0A432FBK0" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432FBK0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FBK0" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" } }, "id": "GLJLFKCOHC_656", "locus": "GCA013368035_00642" }, { "type": "cds", "sequence": "contig_1", "start": 372441, "stop": 372719, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGGCCAGTTGTTTTTGGTCATCATGTTAGTTTAGATACTGGTACTGGCTTAGTTCACATTGCTCCATTATTTGGTGAAGATGACTTTATTATCGGCAAAAAAGAAAATCTAGAAATGATTATGCACATAAACGATGATGGAACAATTAATGATAAAGGTTTTGAATATAATGGTGTTTTTTACGATGATGCAAATCCTTTAATTGGCAAATTTTTAGCAGAAAATAATCAATTAATTGCGTTTAAGAAAATTAAACACTCATATCCTCACGATTGA", "aa": "MGPVVFGHHVSLDTGTGLVHIAPLFGEDDFIIGKKENLEMIMHINDDGTINDKGFEYNGVFYDDANPLIGKFLAENNQLIAFKKIKHSYPHD", "aa_hexdigest": "2b5dcf0f73acb3759ac1627b6a14434d", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10286.513699999989, "isoelectric_point": 5.131447029113769 }, "id": "GLJLFKCOHC_657", "locus": "GCA013368035_00643" }, { "type": "cds", "sequence": "contig_1", "start": 372798, "stop": 372920, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGGCTAACTCTGGAGTTGCAATTAATGATCAATTTGAATATGCAAAAGTGTTAGTAAATGGCAATAAATATATAATTGCAAAAGATTTAGTTGAATCAGTTGCTGCAGTTGCTAAATGA", "aa": "MLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAK", "aa_hexdigest": "31f4ca199c84f3b0ae8203bebb5441e0", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4267.897200000002, "isoelectric_point": 5.970674705505371 }, "id": "GLJLFKCOHC_658", "locus": "GCA013368035_00644" }, { "type": "cds", "sequence": "contig_1", "start": 373074, "stop": 373337, "strand": "-", "frame": 3, "gene": null, "product": "class I tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25", "UniRef:UniRef90_UPI0023AA8A25" ], "nt": "TTGCCTATTGAACATAAAATGTTGCTAGAAGCTAAAAAGAATGCTAAAGATTTTAGTGTTATTGAATTAAGAAAGAAGGCAGCAGAATATGCTTTAAGTCAAGTTGAACATCAAAAGGCTCAATTTAAAAAATTAAGTTTATTGACAGACTTTTCAGAAATTTATGTGACATTGGATAAAAACTTAGAATTTAAGCAATTAGAACTTTTTGCTAAAATGGTTAAAGAAGGCCTAATTTATCGCGATTTGAAACCAGTTTATTGA", "aa": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLLTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVY", "aa_hexdigest": "3a4646ba2f4069d42af0db44b32f3f27", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 372771, "stop": 373337, "observations": { "mutation": [ 372800, 372926, 372941, 373076 ], "stop": [ 372800, 372926, 372941, 373076 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AA8A25", "query_cov": 1.0, "subject_cov": 0.4484536082474227, "identity": 0.9890000000000001, "score": 166.0, "evalue": 1.62e-50, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MPIEHKMLLEAKKNAKDFSVIELRKKAAEYALSQVEHQKAQFKKLSLFTDFSEIYVTLDKNLEFKQLELFAKMVKEGLIYRDLKPVYWSPSSQSALAEAEVEYADHVSPSLYVAFKVIEGNKYVENGDYLIIWTTTPWTLLANSGVAINDQFEYAKVLVNGNKYIIAKDLVESVAAVAKWEEYQVLSTFKGKEF" }, "paralog": false, "identity": 0.9521276595744681, "score": 341.0, "evalue": 3.41e-122, "description": "Internal stop codon at 372800, 372926, 372941, 373076. Nonsense mutation around 372800, 372926, 372941, 373076.", "psc": { "uniref90_id": "UniRef90_UPI0023AA8A25", "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AA8A25", "UniRef:UniRef50_UPI0023AA8A25" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AA8A25", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AA8A25" ], "product": "class I tRNA ligase family protein" } }, "id": "GLJLFKCOHC_659", "locus": "GCA013368035_00645" }, { "type": "cds", "sequence": "contig_1", "start": 373350, "stop": 373643, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "nt": "ATGGGAGAAAAGAAAGATTATAAAGACACGTTATTGATGCCAAATACTGATTTTCCAATGAAAGCAAATTTAGTTGAAAAAGAATTGGAATATCAAAAGCAATGGCTAGATATTAAGTTGTATCAAACTATTTTAAATAGAAATAAGAACAGCCAATCATTTGTATTACATGACGGTCCGCCATATGCAAATGGCAATATTCATGTTGGACACGCTCTAAACAAAATTTTAAAAGATATAATTGTAAGATTTAGAAGTATGCAGGGATATTATTCTCCATATGTTCCGGGTTGA", "aa": "MGEKKDYKDTLLMPNTDFPMKANLVEKELEYQKQWLDIKLYQTILNRNKNSQSFVLHDGPPYANGNIHVGHALNKILKDIIVRFRSMQGYYSPYVPG", "aa_hexdigest": "b09497288eb8564db0cec776a1894b3b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00190B9126", "query_cov": 0.9381443298969072, "subject_cov": 0.9891304347826086, "identity": 0.626, "score": 130.0, "evalue": 1.55e-37, "valid": false, "product": "class I tRNA ligase family protein", "uniref50_id": "UniRef50_A0A352TTR4" }, "pscc": { "uniref50_id": "UniRef50_A0A352TTR4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A352TTR4" ], "product": "Aminoacyl-tRNA synthetase class Ia domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_660", "locus": "GCA013368035_00646" }, { "type": "cds", "sequence": "contig_1", "start": 373887, "stop": 374102, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08264.19" ], "nt": "TTGATTTTAGAAGTTAGCGAAAATATTGAAAAAACTAAGTTTAATATTGCAATAAGCAAAATGATGGTGTTTACTAATTACTTAACGTCTTTAAAAACTATTTCATCATCATTTGTTTTAAAACAATTTGCTATTTTATTAAGTCCGTTTGCGCCATTTTTAGCTGAAGAACTTTTGTTTATGTTGAATGAAAAACCAATTCAATATCAAACTTGA", "aa": "MILEVSENIEKTKFNIAISKMMVFTNYLTSLKTISSSFVLKQFAILLSPFAPFLAEELLFMLNEKPIQYQT", "aa_hexdigest": "16ae58fe074e9ca7225a566ad71b759c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08264.19", "name": "Anticodon_1", "length": 85, "aa_cov": 0.9014084507042254, "hmm_cov": 1.0, "evalue": 1.024010444253231e-10, "score": 37.083045959472656, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 8195.668099999994, "isoelectric_point": 6.140395927429199 }, "id": "GLJLFKCOHC_661", "locus": "GCA013368035_00647" }, { "type": "cds", "sequence": "contig_1", "start": 374229, "stop": 374423, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00133.28" ], "nt": "TTGGGTATTGTTAATACAAAAGAACCATTTACAAAGCTAATAAACCAAGGATTAATTATGGGACCTGATGGTCAAAAAATGTCTAAATCTCTAGGTAATGTTATAAATCCTGATGATTTTATTTTATCTCATGGAGCAGATAGTTTAAGAGTTTATGAAATGTTTATGGGTCCTTTGGTGGAATCAAAATCTTGA", "aa": "MGIVNTKEPFTKLINQGLIMGPDGQKMSKSLGNVINPDDFILSHGADSLRVYEMFMGPLVESKS", "aa_hexdigest": "7700a8b03e05cbedaece89c5a097eac2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00133.28", "name": "tRNA-synt_1", "length": 48, "aa_cov": 0.75, "hmm_cov": 1.0, "evalue": 7.067628788718925e-16, "score": 53.310489654541016, "start": 5, "stop": 52 } ], "seq_stats": { "molecular_weight": 6998.0638, "isoelectric_point": 5.587352561950683 }, "id": "GLJLFKCOHC_662", "locus": "GCA013368035_00648" }, { "type": "cds", "sequence": "contig_1", "start": 374832, "stop": 375086, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "ATGTCATATGGAAGCGGGGCAGTAATGTGCGTGCCTGGTCATGATCAAAGAGATTATGAATTTGCTAAAAAATTTGATTTAAAGATTAAAAAAATTATTGAATGTGATGAATTACCATTTTTAGGAGATGGTATTCATATCAATTCAGATATTGCAAATGGTTTGGATAATGCAGGGGCAGTAAAATGTATTTTTAATGAATTGAAAGCAAATAATTTGGCAGATGAAAAAGTAAGTTATAAATTACGCGATTGA", "aa": "MSYGSGAVMCVPGHDQRDYEFAKKFDLKIKKIIECDELPFLGDGIHINSDIANGLDNAGAVKCIFNELKANNLADEKVSYKLRD", "aa_hexdigest": "feb5be2e345d142cf793d6cf1fd3e4d3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 77, "aa_cov": 0.8095238095238095, "hmm_cov": 1.0, "evalue": 5.295369025277847e-19, "score": 63.81592559814453, "start": 1, "stop": 68 } ], "seq_stats": { "molecular_weight": 9322.527299999992, "isoelectric_point": 5.265132331848144 }, "id": "GLJLFKCOHC_663", "locus": "GCA013368035_00649" }, { "type": "cds", "sequence": "contig_1", "start": 375105, "stop": 375353, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13603.12" ], "nt": "TTGCAACTCTCAACATTTACTACTAGATTGGATACTATCTATGGTGTAACTGCAATAGTAATTTCTCCCGAACACCCACTTCTTCAAAAAATAACTACCGCAAAATATCATGATGATATTTCTAACTATTTAAAAGAAGTTAAATTGAAAAATGAATTAGAAAGAACATCTTTAAATAAAGATAAAACAGGATATTTTACTGGTTCGTATGCAATTCATCCATTGACTAATGAATTAATTCCTATTTGA", "aa": "MQLSTFTTRLDTIYGVTAIVISPEHPLLQKITTAKYHDDISNYLKEVKLKNELERTSLNKDKTGYFTGSYAIHPLTNELIPI", "aa_hexdigest": "191f04c27562310af5aaf71b65e975e4", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13603.12", "name": "tRNA-synt_1_2", "length": 81, "aa_cov": 0.9878048780487805, "hmm_cov": 0.9753086419753086, "evalue": 2.9623127341994744e-24, "score": 80.93769073486328, "start": 2, "stop": 82 } ], "seq_stats": { "molecular_weight": 9353.616299999994, "isoelectric_point": 6.8211565017700195 }, "id": "GLJLFKCOHC_664", "locus": "GCA013368035_00650" }, { "type": "cds", "sequence": "contig_1", "start": 375855, "stop": 375977, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTACCCTTCGGGAATGGGATTGCACGTTGGGCATTTAGAGGGTTATACCGCTACTGATATTATTGCTAGGTATAAACGCCTAAATGGTTTTGATGTGTTGCATCCTATTGGCTGA", "aa": "MFPYPSGMGLHVGHLEGYTATDIIARYKRLNGFDVLHPIG", "aa_hexdigest": "21af7fc6286f9ee63374d2bcb75ef168", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4445.087, "isoelectric_point": 6.810016059875489 }, "id": "GLJLFKCOHC_665", "locus": "GCA013368035_00651" }, { "type": "cds", "sequence": "contig_1", "start": 376360, "stop": 377586, "strand": "+", "frame": 1, "gene": null, "product": "Lmp related protein", "db_xrefs": [ "RefSeq:WP_175949880.1", "SO:0001217", "UniParc:UPI00158C63D9", "UniRef:UniRef100_UPI00158C63D9", "UniRef:UniRef50_A0A454CAH5", "UniRef:UniRef90_D1J895" ], "nt": "ATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAAACATTTAATGATTTAAAGAATAAATTGCAAGATCTATTGAACTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTGCAAACACAATAATTGATGATAATTCTCAAATACAAGACATTGAAAATAAAATCAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCTTAATAAAACTAAGACAAGATTTTAACAAAGCAAAAGAAGATCTAAAAAATCTTTTGGAATCAAAAGAAGTAGCGGATATTGAAAAAGAAACCGAATTAAATACTCTAAATACAACTGATATTTCCAAAGATTCTTTACAACAAGAAATTCAAACCAAAATACAAAAAATTCAAGAAGCTATACAATCAATTAACAAAAAAATCAATGACAAAAAGCAATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAAGACACTTGAAAATTTAATTGCTACAGAAGATGCTAAAACAGTTGGAATTGATGAAGCAAAAAAAGTTCTTGACAATAATAAAAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAACGATTTGAACGATATTACAGCCAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAACTTTCAAAAAATCTAAACAAGATTTAGAAGAATATATAAATAAAAATTTAAATGAAATCAGATTTATTGAAGTTAAAGAAAAAGCCCAAAAAACCATTGAAAAATTTTCTAATATAAACAAAGATAATTCCTCAATCAAAGAAATTGAAGATGCTACTAGCTCGCTAAATAAAGCTAAAGAAGTTGCTTTGAAAGAAGAAGAAGAAATCAGAAAATTAAACGAAGTAATAAATTTTATTGCCAAAAGAAATGAACTAGATAAATTAATAAAATCATCTAATATTAAATCTAGTGAAGATGTTATGAAAAAGGCTAAAAAAACTTTAGACGATAATATGTCTGTTGATACTAATAAATTATCTTCGAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAGAAGCAATAAATGAACTAAAGGCAGCATTAGAAGCAACAAAAAAATCTAAAAATTAA", "aa": "MKRKILLFTPLIIAGSLIPTTLTACNNEDSKLTKQLKAKQTFNDLKNKLQDLLNSEDAKDIDANKEINTLANTIIDDNSQIQDIENKIKTIEDAIESLTKKINDKKILIKLRQDFNKAKEDLKNLLESKEVADIEKETELNTLNTTDISKDSLQQEIQTKIQKIQEAIQSINKKINDKKQLNKEQQNLVEAKKTLENLIATEDAKTVGIDEAKKVLDNNKNNKISTIEEIKKAINDLNDITAKLNQKIANKKQELLETFKKSKQDLEEYINKNLNEIRFIEVKEKAQKTIEKFSNINKDNSSIKEIEDATSSLNKAKEVALKEEEEIRKLNEVINFIAKRNELDKLIKSSNIKSSEDVMKKAKKTLDDNMSVDTNKLSSKDFEEKRKNIEEAINELKAALEATKKSKN", "aa_hexdigest": "aa855dfe034551e64780d7bf0cad2469", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00158C63D9", "ncbi_nrp_id": "WP_175949880.1", "uniref100_id": "UniRef100_UPI00158C63D9", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C63D9", "RefSeq:WP_175949880.1", "UniRef:UniRef100_UPI00158C63D9" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C63D9", "uniref90_id": "UniRef90_D1J895" }, "psc": { "uniref90_id": "UniRef90_D1J895", "product": "Lmp related protein", "uniref50_id": "UniRef50_A0A454CAH5" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAH5" ], "product": "Lmp protein" }, "genes": [], "id": "GLJLFKCOHC_666", "locus": "GCA013368035_00652" }, { "type": "cds", "sequence": "contig_1", "start": 377795, "stop": 377968, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C2CEE", "UniRef:UniRef90_UPI000A5C2CEE" ], "nt": "ATGGGTAAAAAAGCAAAATTATTTGTCTTTGGCGGGATTAGTTTTTTAGCGGGAGCTTCAATTATTGGTTTTGTTGCAGCATATGCTTCAATTAATAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTGACAATTGA", "aa": "MGKKAKLFVFGGISFLAGASIIGFVAAYASINKKNEIRVSEYNKFLEEKTNLNSFDN", "aa_hexdigest": "d7e91a5298bad7b233a85da8e12dba64", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 377795, "stop": 378162, "observations": { "deletion": [ 378122 ], "mutation": [ 377966 ], "stop": [ 377966 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C2CEE", "query_cov": 1.0, "subject_cov": 0.4672131147540984, "identity": 0.912, "score": 101.0, "evalue": 1.33e-26, "gene_start": 1, "gene_end": 57, "reference_start": 1, "reference_end": 57, "reference_sequence": "MGKKAKLFVFSGISFLVGASIIGSIAAYASINKKNEIRVSEYNKFLEEKTNLNSFNNLENDKKISSIKSNTLSEVEKIDKNSDLFTIQKARKIVNESIENIKNIIASHKKNIIRKRKSVYSI" }, "paralog": false, "identity": 0.9344262295081968, "score": 213.0, "evalue": 2.84e-73, "description": "Internal stop codon at 377966. Frameshift due to deletion around 378122. Nonsense mutation around 377966.", "psc": { "uniref90_id": "UniRef90_UPI000A5C2CEE", "uniref50_id": "UniRef50_UPI000A5C2CEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C2CEE", "UniRef:UniRef50_UPI000A5C2CEE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C2CEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C2CEE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6292.176999999998, "isoelectric_point": 9.456109809875489 }, "id": "GLJLFKCOHC_667", "locus": "GCA013368035_00653" }, { "type": "cds", "sequence": "contig_1", "start": 378125, "stop": 378283, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAGCAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTGAATAAAGAAAGCCAAGAACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGA", "aa": "MLLEKEKAFIQYSNVKKQLQDMLLKIFDFLNKESQEHYYIGDGNEKIINKQN", "aa_hexdigest": "4c070a38b52a4ddb442443766bdf620e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6279.1569, "isoelectric_point": 6.545942115783691 }, "id": "GLJLFKCOHC_668", "locus": "GCA013368035_00654" }, { "type": "cds", "sequence": "contig_1", "start": 378548, "stop": 378754, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTCGTATATTTTTTATTTAAATACAAAAATGATGACAAAAAAATATTTACAAAATTTAAAAATTATGCACAAGAAATGTATGACAAAACTGCACTTCTTGATGAATTCGACATTGATTTTTTAGGATTTAAAATCAAGGATAAGAACTTCTATAAAAATTATATTGATTTGTTTGCACCATATAAAAAGGCTATTGATTTGTGA", "aa": "MFVYFLFKYKNDDKKIFTKFKNYAQEMYDKTALLDEFDIDFLGFKIKDKNFYKNYIDLFAPYKKAIDL", "aa_hexdigest": "a698ea79b511dea6831d878c1ba915f1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8423.702299999995, "isoelectric_point": 8.729680061340332 }, "id": "GLJLFKCOHC_669", "locus": "GCA013368035_00655" }, { "type": "cds", "sequence": "contig_1", "start": 378818, "stop": 379048, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E", "UniRef:UniRef90_UPI0024112F2E" ], "nt": "TTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGATAAATCAACCCGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTATTCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAATGACAATCAATAA", "aa": "MYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLIPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ", "aa_hexdigest": "d360ff4b85445756d6d1efd4595df859", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 378728, "stop": 379048, "observations": { "mutation": [ 378752, 378809 ], "stop": [ 378752, 378809 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0024112F2E", "query_cov": 1.0, "subject_cov": 0.7037037037037037, "identity": 0.9740000000000001, "score": 154.0, "evalue": 2.63e-47, "gene_start": 1, "gene_end": 76, "reference_start": 33, "reference_end": 108, "reference_sequence": "MTPYKKAIDLWKNYYDSYSIGLKEVEEIIWDKLYINGYTIFFKPKKIYALINQPELDKELTETTDDYKFKLMLVPKENTIEHNKLIENFKNYCDDLMNRSKKLKNDNQ" }, "paralog": false, "identity": 0.9528301886792453, "score": 210.0, "evalue": 8.05e-72, "description": "Internal stop codon at 378752, 378809. Nonsense mutation around 378752, 378809.", "psc": { "uniref90_id": "UniRef90_UPI0024112F2E", "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0024112F2E", "UniRef:UniRef50_UPI0024112F2E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0024112F2E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0024112F2E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9208.525799999992, "isoelectric_point": 7.784702491760254 }, "id": "GLJLFKCOHC_670", "locus": "GCA013368035_00656" }, { "type": "cds", "sequence": "contig_1", "start": 379062, "stop": 379481, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAAGAGCAAAAATTCTAACCTCATTAAGTCTTTGCTTAACTACTGCTATTTCAACAATAGCAATCTATTCAATAATCGCCGTCAAAAAAAATATAGAACTAGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAGATTGCAATCGCTTGTTGCAAATAATCAACACAAAATTCCAAATAGCTATTTAAATTCAATTCAATATTTTCATAATATAACTAAAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAGGGAAATTATTGACGTAAATAAAAAAATTCTTGCCTTTGATTTAGAAATAAAAAATCAATTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTACCATAACAGACTATATAAAATATGA", "aa": "MKKRAKILTSLSLCLTTAISTIAIYSIIAVKKNIELDKSNKYSLFIQEKSRLQSLVANNQHKIPNSYLNSIQYFHNITKTSKAIEIIDATSKIRREIIDVNKKILAFDLEIKNQLSLFNEFNQIKQKLENDYHNRLYKI", "aa_hexdigest": "27110449f7d9e308827a17fa7f14262b", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16202.720599999988, "isoelectric_point": 9.78676815032959 }, "id": "GLJLFKCOHC_671", "locus": "GCA013368035_00657" }, { "type": "cds", "sequence": "contig_1", "start": 379974, "stop": 380381, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTACTCACCTTTATATTTTTATGAACCAACAGAAGAAATCAAAAAAGTTTTCCAATATCCTGATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAGATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCCTACAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACCAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTCGAAATAAAAAAAACCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTTAAAATATTCCACAAGAGATAAAAAGAGTATTTGA", "aa": "MFFYSPLYFYEPTEEIKKVFQYPDKKDFEKEIILKYNQAKQDLTNFKSKINGNEKIAKKAYNKFNEYIRNLAISFEKELIQKKLLLLKNLLTKYDQKQDGKSQFITVEIKKTINDAESLLSYNDLKYSTRDKKSI", "aa_hexdigest": "bec26daea972ccb430cc63f4a91ad9da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16205.50909999999, "isoelectric_point": 9.397894859313965 }, "id": "GLJLFKCOHC_672", "locus": "GCA013368035_00658" }, { "type": "cds", "sequence": "contig_1", "start": 380551, "stop": 380790, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961", "UniRef:UniRef90_UPI00215DA961" ], "nt": "TTGAAATTGATATCATCAAATGTTGAGGAATTTAATATTCTTGCAAATGATGTCTATTCTAAAAATCAAAAAATTTCTAACGAAATACAAGAAGTTAGAAAACTTATAGAAGAACACGAAGAAAAATTAGAAGATTTTTATATTAAGAAATCACAGCCAAATTTAAAATATTTAATCGAGATATTACTTAAATTACAAAATAAACTGTATTTAATTAGACAAAAAGTCAATCAAATTTAA", "aa": "MKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHEEKLEDFYIKKSQPNLKYLIEILLKLQNKLYLIRQKVNQI", "aa_hexdigest": "c330bf19e9a2e4610edc634705fdf5b3", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 380551, "stop": 381000, "observations": { "mutation": [ 380799 ], "stop": [ 380799 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA961", "query_cov": 1.0, "subject_cov": 0.5302013422818792, "identity": 0.924, "score": 134.0, "evalue": 8.7e-39, "gene_start": 1, "gene_end": 79, "reference_start": 71, "reference_end": 149, "reference_sequence": "MLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSKLFSSSDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVKKLIEEHDKKLEDFYIKKLQPNLKYLTEILLKLQNKLYLIRQKVNQI" }, "paralog": false, "identity": 0.9463087248322147, "score": 270.0, "evalue": 7.25e-95, "description": "Internal stop codon at 380799. Nonsense mutation around 380799.", "psc": { "uniref90_id": "UniRef90_UPI00215DA961", "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA961", "UniRef:UniRef50_UPI00215DA961" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA961" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9534.981699999991, "isoelectric_point": 7.9171850204467775 }, "id": "GLJLFKCOHC_673", "locus": "GCA013368035_00659" }, { "type": "cds", "sequence": "contig_1", "start": 380797, "stop": 381387, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B", "UniRef:UniRef90_UPI002410C12B" ], "nt": "ATGTTGAATGAATTATTTAATGATTTTAAGGATATTGATTCAAAAACATTTGCAGAAGTTATTTATCAATTTGAAGAATCGAATAAATTATTGGCCGAATATATTGAATTATATTCACAAATTATTTATAATGCAAAGAATGAATTTGAAATGCAACAAAAAAATAGCGATTTATTTAATTTATTTAGTCAAGAAACAAAAAATAAAATTACAGATTTTTTAGCAACAATTAATAAATTGGAAAACAATAGAAATGATCAACAAATAAAAAAATCTATACATGAAGAATTTAAAAAAATTCTAACTGTCCAAGAAGAAATTTATAAAAATATAAATAAGGATTTTTTAAACATTACTAATAAAGAAGAAAGCATTAAACTTTTAAAAATGCTTAATGAAAGTTTAAAAGAACAAAAAAATTCAGAAGATGGAAATTTTTTAAGTAAATTTCTAAATAATGAAATAGCCAAAATATTAAAAGAAAATTCTGAGTTATTTTCTTCAAAAGATATTGCCAAAATCAATAAAAATATAGAAATTACATACGAACTTTCTAGAAAATTAAACTTAGAAATTATTTTAAACAGGTGA", "aa": "MLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITDFLATINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIKLLKMLNESLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEITYELSRKLNLEIILNR", "aa_hexdigest": "dc960ea7aa1df18e2f3e77a49e05fa15", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 380557, "stop": 381687, "observations": { "mutation": [ 380799, 381408, 381651 ], "stop": [ 380799, 381408, 381651 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410C12B", "query_cov": 1.0, "subject_cov": 0.47115384615384615, "identity": 0.98, "score": 351.0, "evalue": 1.53e-118, "gene_start": 1, "gene_end": 196, "reference_start": 141, "reference_end": 336, "reference_sequence": "MKSKEILWNISVQKNLIDQRYTKESLSPKSKIDVEFLKQKRKLENLFSIYKKWNKADMSKEIELLKKINNVNFGFKNDDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKTWDVVDRLMLNELFNDFKDIDSKTFAEVIYQFEESNKLLAEYIELYSQIIYNAKNEFEMQQKNSDLFNLFSQETKNKITDFLAIINKLENNRNDQQIKKSIHEEFKKILTVQEEIYKNINKDFLNITNKEESIRLLKMLNKNLKEQKNSEDGNFLSKFLNNEIAKILKENSELFSSKDIAKINKNIEITYELSRKLNLEIILNRWNLLKLISSNVEEFNILANDVYSKNQKISNEIQEVRKLIEEHEEKLEDFYIKKSQLILKYLTEILLKLQNKLHSIRQKVN" }, "paralog": false, "identity": 0.9654255319148937, "score": 694.0, "evalue": 1.86e-256, "description": "Internal stop codon at 380799, 381408, 381651. Nonsense mutation around 380799, 381408, 381651.", "psc": { "uniref90_id": "UniRef90_UPI002410C12B", "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410C12B", "UniRef:UniRef50_UPI002410C12B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410C12B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410C12B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 23326.227300000035, "isoelectric_point": 5.226368141174315 }, "id": "GLJLFKCOHC_674", "locus": "GCA013368035_00660" }, { "type": "cds", "sequence": "contig_1", "start": 381406, "stop": 381636, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGCAAAGAAATAGAACTTCTTAAAAAGATTAATGATGTTAATTTTGGTTTTAAAAATGATGATATAGCGAAATTAACTAGTGAAATTTCAAAAAGAATTGAAGATATTTATCAAAAAATAAATTCATCAAAAGATATACAAAAACATTTAAATAATGTTAAAAATGATTTAAATCCTCTTATACAAGAATTGATTAACTTTAATAATTTTAAAAACAGTAAAACATGA", "aa": "MSKEIELLKKINDVNFGFKNDDIAKLTSEISKRIEDIYQKINSSKDIQKHLNNVKNDLNPLIQELINFNNFKNSKT", "aa_hexdigest": "3ba5dec5ea2fbb62f273ee7ba9a54bf6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8894.042699999995, "isoelectric_point": 8.926566505432131 }, "id": "GLJLFKCOHC_675", "locus": "GCA013368035_00661" }, { "type": "cds", "sequence": "contig_1", "start": 381784, "stop": 382239, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACGAAAAAACAAACTATTTATTCTATCAACTATCTTAGGATCGTTTGCAACTGTATTTACAGTAATTTCTAGTGCTTCATGTTCTAATAAACAAGATAAAAAAGAAGAAACTAAAGATAAAAAAGAAGATTCAAACAACAATGTAGCAAATACGCCTAATGTCAAACCAAATATATCTAAAGAAAAACAAGAAGATAATAACCACATAGTTAGTCCTAATATAAAGCCAACTGTTCCAAACGAATCTCAAAAACCAAAAGAAGATAAAAATAATGCGACAAGTTCTAGCCCTAGTAGGTTACAAGAAGTTAAAAATGAACTTCAAACATATATAGCCAATAATCTTAAAGATTTTATTTATGAAAATTTAAAATTAGAAATCAAAGATTTCATTGAAAAAGAAAAGAATGAGGCAGATGAATTTATTTTGAAATCTAAAGAAATTCTTTGA", "aa": "MKRKNKLFILSTILGSFATVFTVISSASCSNKQDKKEETKDKKEDSNNNVANTPNVKPNISKEKQEDNNHIVSPNIKPTVPNESQKPKEDKNNATSSSPSRLQEVKNELQTYIANNLKDFIYENLKLEIKDFIEKEKNEADEFILKSKEIL", "aa_hexdigest": "024834e77ed5193d07dab5fc2fc5eab9", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17244.2079, "isoelectric_point": 8.498883247375488 }, "id": "GLJLFKCOHC_676", "locus": "GCA013368035_00662" }, { "type": "tRNA", "sequence": "contig_1", "start": 382450, "stop": 382533, "strand": "+", "gene": "trnY", "product": "tRNA-Tyr(gta)", "amino_acid": "Tyr", "anti_codon": "gta", "score": 70.9, "nt": "GGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCA", "db_xrefs": [ "SO:0000272" ], "anti_codon_pos": [ 382456, 382458 ], "id": "GLJLFKCOHC_20", "locus": "GCA013368035_00663" }, { "type": "tRNA", "sequence": "contig_1", "start": 382537, "stop": 382611, "strand": "+", "gene": "trnQ", "product": "tRNA-Gln(ttg)", "amino_acid": "Gln", "anti_codon": "ttg", "score": 65.1, "nt": "TGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCA", "db_xrefs": [ "SO:0000261" ], "anti_codon_pos": [ 382569, 382571 ], "id": "GLJLFKCOHC_21", "locus": "GCA013368035_00664" }, { "type": "cds", "sequence": "contig_1", "start": 382716, "stop": 383078, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145", "UniRef:UniRef50_UPI001C669CA5", "UniRef:UniRef90_UPI001C669CA5" ], "nt": "ATGGATGCTCTTGAGTTACAAGAACAAGGAATACAGGAAGATAAATTAGAAAAATTTAAATCTGATTTTAGAGCATTAAAAAAACCAATTGAAGATAAGATGAATGAAATTATTGGTGAATTTTTTGATTTCATAAATAATTTGTATGGCACAAATAGTAATACATCTTCTTTTATTGAAAAAAATCTTAAAGGAAAAGTTGAATATAAAGAAGGCTACGAAGCTTTAAACAAATTAATACAAGAAGCAGAAAAAATTTTGGACAACCCAGGAGAACATAGCAATTTAGAATTAAAAAATAAAGAAGCTGAAATACAAGCCAAATTACTTGAATATAAAAATAAATATAATATGAACAAATAA", "aa": "MDALELQEQGIQEDKLEKFKSDFRALKKPIEDKMNEIIGEFFDFINNLYGTNSNTSSFIEKNLKGKVEYKEGYEALNKLIQEAEKILDNPGEHSNLELKNKEAEIQAKLLEYKNKYNMNK", "aa_hexdigest": "7bd768f99511155d656ec2c644e13d18", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI00093A2145", "query_cov": 0.9916666666666667, "subject_cov": 0.815068493150685, "identity": 0.773, "score": 169.0, "evalue": 5.99e-52, "valid": false, "uniref50_id": "UniRef50_UPI00093A2145" }, "pscc": { "uniref50_id": "UniRef50_UPI00093A2145", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00093A2145" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 382716, "stop": 383345, "observations": { "mutation": [ 383114, 383252 ], "stop": [ 383114, 383252 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001C669CA5", "query_cov": 1.0, "subject_cov": 0.5741626794258373, "identity": 0.958, "score": 218.0, "evalue": 3.03e-70, "gene_start": 1, "gene_end": 120, "reference_start": 90, "reference_end": 209, "reference_sequence": "KKIIDHLNAQLNLFKDITSASDIQQISVAKQWLTKDLNNAKEDKFSCDISCFVNLEVPEFIGDMQSKSLTCLDSKKYWEEPKKEIERIRMDALDLERQGIQEDKLEKFKSDFRALKKPIEDKMNEIIGEFFDFINNLYGTNSNTSSFIENKLKGKVEYKEGYEALNKLIQEAEKILDNPGEHSNLELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.9665071770334929, "score": 399.0, "evalue": 2.12e-144, "description": "Internal stop codon at 383114, 383252. Nonsense mutation around 383114, 383252.", "psc": { "uniref90_id": "UniRef90_UPI001C669CA5", "uniref50_id": "UniRef50_UPI001C669CA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001C669CA5", "UniRef:UniRef50_UPI001C669CA5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001C669CA5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001C669CA5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 14038.692799999983, "isoelectric_point": 5.059090995788574 }, "id": "GLJLFKCOHC_677", "locus": "GCA013368035_00665" }, { "type": "cds", "sequence": "contig_1", "start": 383250, "stop": 384140, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193B9E77", "UniRef:UniRef90_UPI00193B9E77" ], "nt": "TTGCAAAAATTAATTGATGCAGCCAAAGAAAATGCTAAACAAAACTTTGATTCAAAGAAACAACAACTAGATAATTTGATTAAATCAAATGAAGCCAAAGATGTTGATAAACAATCAGAAACCAACATCTTTAATAACACAAATCTTACTAACAATGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGACTTTAATGACGCTAAAAAGCAATTAGAAGACTTAATCAATTCACAAGACGCACAAAAAGTCGACAAATCTAATGCAAATCAATCATTGAAAAATAACAATGTAGATATTTCATCGACGACCGATCAAATTGTGAATGCAACAAATGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAAAGCCACACAAGACTTTAATTTAAAGAAACAACAATTAGATAATTTAATTAAATCAAATGAAGCTAAAGATGTTGATAAACAAGAAGAAACCAACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAGTCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAGTTAATGATAAAAAACCAAAGGAAAATATTGAATATGAAGGGCTTGAAAAAATTAGGGAACAAATTAAACAATTTATTGAAAAAGTAAAAGAGGATGAATATTATAAAGAATATAATAGTAACTATTATGATTATAAAAAAATAATAGACCATTTAAACGCACAACTTAATCTCTTTAAAGATATTACTTCTGCTTCTGACATTCAACAAATATCCGTGGCTAAGCAATGA", "aa": "MQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDKSNANQSLKNNNVDISSTTDQIVNATNEIKKATQDLQKLIDAAKEKATQDFNLKKQQLDNLIKSNEAKDVDKQEETNIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKVNDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYKEYNSNYYDYKKIIDHLNAQLNLFKDITSASDIQQISVAKQ", "aa_hexdigest": "9d40e3ec8255c1931c4f661912e2d2f4", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 382947, "stop": 384440, "observations": { "mutation": [ 383114, 383252, 384197 ], "stop": [ 383114, 383252, 384197 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193B9E77", "query_cov": 1.0, "subject_cov": 0.4525993883792049, "identity": 0.882, "score": 415.0, "evalue": 6.84e-139, "gene_start": 1, "gene_end": 296, "reference_start": 181, "reference_end": 476, "reference_sequence": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEAKDVDKQSETNIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDTSWTIDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDTSWTIDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYKEHNSKYYDYKKIIDHLNQQLMILENVTSASDIQQISGAKQWLTKDLNNAKEDKFSCDIYCFVNIEVFEFITNMQNNSLTCLDSKKYWEEPKKEIERIRMAALELQEQGIQEDKLEKFKSDFRASKKPIEDKMKEIINEFFDFLDKLSNTKSSTSSFIEKNLKGKAEYKEGYDALNKLIQEAEEILNNLGEHSNLELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.8893360160965795, "score": 674.0, "evalue": 5.63e-243, "description": "Internal stop codon at 383114, 383252, 384197. Nonsense mutation around 383114, 383252, 384197.", "psc": { "uniref90_id": "UniRef90_UPI00193B9E77", "uniref50_id": "UniRef50_UPI00193B9E77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193B9E77", "UniRef:UniRef50_UPI00193B9E77" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193B9E77", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193B9E77" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 34085.55420000002, "isoelectric_point": 5.459521675109863 }, "id": "GLJLFKCOHC_678", "locus": "GCA013368035_00666" }, { "type": "cds", "sequence": "contig_1", "start": 384195, "stop": 384575, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAATTAATTGATGCAGCCAAAGAAAATGCTAAACAAAACTTTGATTCAAAGAAACAACAACTAGATAATTTGATTAAATCAAATGAAGCCAAAGATGTTGATAAACAATCAGAAACCAACATCTTTAATAACACAAATCTTACTAACAAAGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGATGGTCAAAAAGTTGACACTTCTAAAGCAAATCAATCATTGCAAAACAATAATGTAGATACTTCATGA", "aa": "MQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDTS", "aa_hexdigest": "d3eae2460183b204ad961953032325f9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14325.714499999975, "isoelectric_point": 7.7970159530639656 }, "id": "GLJLFKCOHC_679", "locus": "GCA013368035_00667" }, { "type": "cds", "sequence": "contig_1", "start": 384630, "stop": 386411, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158D8503", "UniRef:UniRef90_UPI00158D8503", "PFAM:PF10732.14" ], "nt": "ATGAAAAAATCGAAATTAAGAATTTTACTTTCTACTTCGTTAATTATTGCTCTAGCAACAACGGCAACCTTAGTTGCAACATCACTAAAAAGTAAATTTAAAAGAAGACAATATGAACAAGAAGATAATATTGACAAATTAAAGGAAAAATTTAATAGATCAAAATCTAAATTAGATAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAACCAGAAACTGACATCTTTAACAATACAAATCTTGCTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACCATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATCAATTCACAAGACGCACAAAAAGTCGACACTTCTAAAGCAAATCAATCATTGCAAAACAACAATGTAGATACCTCATCAACGACTGATCAAATTATTAATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAACAAGCCAAACAAGACTTTAATTCAAAGAAGCAACAATTAGATAATTTAATTAAATCAAATGAAGCCAAAGAAGTTGATAAACAACCAGAAACTGACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTAACTAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGACTTTAATGATGCCAAAAAGCAATTAGAAGACTTAATTAAATCACAAGATGCACAAAAAGTTGACACATCTAAAGCAAATCAATCATTGCAAAACAATAATGTAGATACTTCATCAACGACTGATCAAATTATTAATGCAACAACTGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCTAAAGAAAAAGCCACACAAGACTTTAATTCAAAGAAACAACAACTAGATAATTTAATTAAATCAAATGAAGCCAAAGATGTTGATAAACAATCAGAAACCAACATCTTTAACAATACAAATCTTACTGGTAATGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTGAATCATTGACCAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGATTTTAATGACGCTAAAAAGCAATTAGAAGACTTAATCAATTCACAAGACGCACAAAAAGTCGACACTTCTAAAGCAAATCAATCATTGCAAAACAATAATGTAGATACTTCATCAACGATCGATCAAATTGTTAATGCAACAAATGAAATTAAAAAAGCCACACAAGATTTGCAAAAATTAATTGATGCAGCCAAAGAAAATGCTAAACAAAACTTTGATTCAAAGAAACAACAACTAGATAATTTGATTAAATCAAATGAAGCCAAAGATGTTGATAAACAATCAGAAACCAACATCTTTAATAACACAAATCTTACTAACAAAGATTTAATTAAAGACATTGAATCAAAAACAAAAACTATTGAAGATGCTATTAAATCATTAACTAAGAAAATTAATGATAAAAAGAACCAAAAAGACAATCTTCTTAAAGATTTTAATGATGCTAAAAAGCAATTAGAAGACTTAATTAATTCACAAGATGGTCAAAAAGTTGACACTTCTAAAGCAAATCAATCATTGCAAAACAATAATGTAGATACTTCATGA", "aa": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEAKDVDKQPETDIFNNTNLAGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDTSSTTDQIINATTEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSNEAKEVDKQPETDIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLIKSQDAQKVDTSKANQSLQNNNVDTSSTTDQIINATTEIKKATQDLQKLIDAAKEKATQDFNSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDTSSTIDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDTS", "aa_hexdigest": "c5d0f78069ac0988e1921159637aabcb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 384375, "stop": 386411, "observations": { "mutation": [ 384632 ], "stop": [ 384632 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158D8503", "query_cov": 0.8988195615514334, "subject_cov": 0.4329813160032494, "identity": 1.0, "score": 658.0, "evalue": 1.49e-221, "gene_start": 1, "gene_end": 533, "reference_start": 1, "reference_end": 533, "reference_sequence": "MKKSKLRILLSTSLIIALATTATLVATSLKSKFKRRQYEQEDNIDKLKEKFNRSKSKLDNLIKSNEAKDVDKQPETDIFNNTNLAGNDLIKDIESKTKTIEDAIESLTKKINDKKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDTSSTTDQIINATTEIKKATQDLQKLIDAAKEQAKQDFNSKKQQLDNLIKSNEAKEVDKQPETDIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLIKSQDAQKVDTSKANQSLQNNNVDTSSTTDQIINATTEIKKATQDLQKLIDAAKEKATQDFNSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTGNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDTSKANQSLQNNNVDTSSTIDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDTSWTIDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNKDLIKDIESKTKTIEDAIKSLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDGQKVDTSKANQSLQNNNVDTSWTIDQIVNATNEIKKATQDLQKLIDAAKENAKQNFDSKKQQLDNLIKSNEAKDVDKQSETNIFNNTNLTNNDLIKDIESKTKTIEDAIESLTKKINDKKNQKDNLLKDFNDAKKQLEDLINSQDAQKVDKSNANQSLKNNNVDISSTTDQIVNATNEIKKATQDLQKLIDAAKEKATQDFNLKKQQLDNLIKSNEAKDVDKQEETNIFNNTNLTGNDLIKDIESKTKTIEDAIKSLTKKVNDKKPKENIEYEGLEKIREQIKQFIEKVKEDEYYKEYNSNYYDYKKIIDHLNAQLNLFKDITSASDIQQISVAKQWLTKDLNNAKEDKFSCDISCFVNLEVPEFIGDMQSKSLTCLDSKKYWEEPKKEIERIRMDALELQEQGIQEDKLEKFKSDFRALKKPIEDKMNEIIGEFFDFINNLYGTNSNTSSFIEKNLKGKVEYKEGYEALNKLIQEAEKILDNPGEHSNLELKNKEAEIQAKLLEYKNKYNMNK" }, "paralog": false, "identity": 0.9970501474926253, "score": 858.0, "evalue": 3.3e-302, "description": "Internal stop codon at 384632. Nonsense mutation around 384632.", "psc": { "uniref90_id": "UniRef90_UPI00158D8503", "uniref50_id": "UniRef50_UPI00158D8503", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158D8503", "UniRef:UniRef50_UPI00158D8503" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00158D8503", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158D8503" ] } }, "hypothetical": true, "pfams": [ { "id": "PF10732.14", "name": "DUF2524", "length": 46, "aa_cov": 0.0775716694772344, "hmm_cov": 1.0, "evalue": 5.699507190454513e-10, "score": 34.828033447265625, "start": 525, "stop": 570 } ], "seq_stats": { "molecular_weight": 66904.52490000021, "isoelectric_point": 5.908720207214355 }, "id": "GLJLFKCOHC_680", "locus": "GCA013368035_00668" }, { "type": "cds", "sequence": "contig_1", "start": 386668, "stop": 387717, "strand": "-", "frame": 2, "gene": null, "product": "MSC_0618 family F1-like ATPase beta subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0004864FE0" ], "nt": "ATGAATTCAACTATAAACAATGAAAGATATCTAGAAAATAACAAAGAAGTTATAGAAACTGGAATAAAGGCAATTGATTTCTTTATGCCAATTGTTAAGGGTGCAAAAATCGGTTTATTTGGAGGAGCCGGAGTTGGAAAAACTGTTCTAATGAAGGAAATTATTTTTAACCTTAATAAACAACATGAAGCAAATTCTAATATCTTTATTGGTTCGGGTGAACGTTCAAGAGAAGGAATAGAATTATACAATGAACTTTCTTCATCTAATTTAATGAAGGACTCAATAATGTTTATTTCAAAAATGAATGAAGCACCAGGAGCTAGAACGTCAATTGTTCCAATCGGAATTACTGCTGCAGAATATTTAAGAGACACATGCAATGAAAATGTTTTATTATTTATTGACAATATTTATCGTTTTATTCAAGCAGAAAATGAAGTAAGTTCAGCATTAGGCAAAAAGCCTTCAATTGGAGGTTATCAATCAACTTTAGAAAGTGATGTAACTGAAGTTGAAAGCAGATTGTTTAGAAACAAGAAGGGCTCAATAACTTCATTCCAAACAATATTTCTACCAATGGATGATTTAAGCGATCCTTCTGCAGTAGCTGTATTTAATCACTTAGATGGTAATTTGGTCTTATCTAGATCTCAAGCAGCTAAAAACATTTTTCCTGCCTTTGATCCACTTGCTTCATCTACAAACGCAATTAATCCAACCATTATTGGCAAGCGTCACTATGATGCAATAATTGAAGCAAAAAGAATATTAAAAGCATATAAAGATCTTGAAGATGTTATTCTGATATTAGGATTTGATGAGCTTGATGAAGAAAGCAAAATCACTGTTAAAAAAGCTCTTCAACTTGAAATGTTTTTTACCCAAAATTTCTTTATGACTGAACATTTTACTAAATCGCCTGGTCAATTTGTGCCATTAAGCGAAACAGTTGAAAGCGTTATAAGAATATTGGAAGGAAGATATTTAAAACAAAATCCTGAAATTTTTGCTTATATAGGTTCAAATAAAGATATTCAACAAGATTAA", "aa": "MNSTINNERYLENNKEVIETGIKAIDFFMPIVKGAKIGLFGGAGVGKTVLMKEIIFNLNKQHEANSNIFIGSGERSREGIELYNELSSSNLMKDSIMFISKMNEAPGARTSIVPIGITAAEYLRDTCNENVLLFIDNIYRFIQAENEVSSALGKKPSIGGYQSTLESDVTEVESRLFRNKKGSITSFQTIFLPMDDLSDPSAVAVFNHLDGNLVLSRSQAAKNIFPAFDPLASSTNAINPTIIGKRHYDAIIEAKRILKAYKDLEDVILILGFDELDEESKITVKKALQLEMFFTQNFFMTEHFTKSPGQFVPLSETVESVIRILEGRYLKQNPEIFAYIGSNKDIQQD", "aa_hexdigest": "6b9c8534eada7b48f60b07b994839b2f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0004864FE0", "query_cov": 0.8997134670487106, "subject_cov": 0.9843260188087775, "identity": 0.7609999999999999, "score": 485.0, "evalue": 2.91e-170, "valid": false, "product": "MSC_0618 family F1-like ATPase beta subunit", "uniref50_id": "UniRef50_UPI0004864FE0" }, "pscc": { "uniref50_id": "UniRef50_UPI0004864FE0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0004864FE0" ], "product": "MSC_0618 family F1-like ATPase beta subunit" }, "genes": [], "id": "GLJLFKCOHC_681", "locus": "GCA013368035_00669" }, { "type": "cds", "sequence": "contig_1", "start": 387763, "stop": 387948, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTGACATTGCATGAAGGAAAAACCTTTTTACTAGTAAAAAGATTAATTGACGAAAAACATGTTAGAGCAATTGTTGTATATGCAAGTAAAGATATTTCAATTAACGAAGAAGTTATTAACACTCATAAGAGTTTTTTGGTTCCAATAGGGCAAAATTCAAAAAACAATATTTACAACTTTTGA", "aa": "MLTLHEGKTFLLVKRLIDEKHVRAIVVYASKDISINEEVINTHKSFLVPIGQNSKNNIYNF", "aa_hexdigest": "11ef20201a3b4ebaaf68904e1c4709e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7040.107099999999, "isoelectric_point": 9.157943534851075 }, "id": "GLJLFKCOHC_682", "locus": "GCA013368035_00670" }, { "type": "cds", "sequence": "contig_1", "start": 388107, "stop": 388289, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGGAATAAAAGATGAACAAAAAGCATTTATGTTACTAGATTATTTAATAAATAATTATAACGATTGCATAAAAGCATTTGAAACAATTTTAAATAATTTAGACTACGATGATAATATTATGAAGAATTATTTTGCATTTGTCTTGAAATCATATTCAGACTATTTAAATTTAAATTGA", "aa": "MQGIKDEQKAFMLLDYLINNYNDCIKAFETILNNLDYDDNIMKNYFAFVLKSYSDYLNLN", "aa_hexdigest": "89190d50c19cbaf243e99e5850e4f55d", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7186.087000000001, "isoelectric_point": 4.268915367126465 }, "id": "GLJLFKCOHC_683", "locus": "GCA013368035_00671" }, { "type": "cds", "sequence": "contig_1", "start": 388296, "stop": 389240, "strand": "-", "frame": 3, "gene": null, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "nt": "ATGTTAAGCAATCATCATCCAATATTCGGTCTAGCTCATAATTTGATGACAGTTAAAACATTAAATGAACAACTTTATACTGGAATTATTGCAATAGATTTATTAATTCCAATTGGAAAAGGTCAACGTGAATTAATTATTGGCGATAGGCAAACAGGAAAAACTCATATGGCATTAAATGCAATTATAAATCAAGCAAAAAATGGCACAAAATGTATTTATGTTGCAATTGGCCAAAAGCGTGAATCATTAACTAATATTTATGAAACATTAAAAAAATACGATGCATTGAAAAACACTATGATTATTGATGCGCCTCAAAACGTTTATGAACAATATCTAGCACCTTACATTGGAATGGCTCATGCAGAAAATCTTTCATATGACAATGATGTTTTAATAGTATTTGATGATTTAACTAAACACGCTAATATTATTAGAGAAATTGCCCTATTAACCAACAAGTCAGTTGGAAAAGAAGCAATGCCTAGCGACATCTTTTTTGCCCACTCATCATTGCTTGAAAGAGCTGGATCTTTTGTTAATAGAAAAACAATTACTGCACTTCCAATTTTGCAAACAGTTGACGGTGATATAACTAGTTTAATTTCATCTAATATAATTTCAATAACTGATGGTCAAATTGTAACTTCTTCTGACTTATTTGCTTCTGGAAAATTACCTGCTATAAACATTGATTTGTCTGTTTCTAGAACAGGTTCAAGTGTTCAATCAAGAATGATGACCAAAATAGCGGGTGAAATTAATAAAATTTTTAGAAGTTACAAGAGACATTTAAAATTGGCAATGTTAGATTACGAGCTTAATAAAGAAACTTCTATTTTGCTTCACAAAGGAAAATTAATTGACAAAATGTTCTTGCAAAAGGGTTTTTCACTTTACTCATATCGTTTTGTAGTGCTAACAACAAAATTAATTTCTTGA", "aa": "MLSNHHPIFGLAHNLMTVKTLNEQLYTGIIAIDLLIPIGKGQRELIIGDRQTGKTHMALNAIINQAKNGTKCIYVAIGQKRESLTNIYETLKKYDALKNTMIIDAPQNVYEQYLAPYIGMAHAENLSYDNDVLIVFDDLTKHANIIREIALLTNKSVGKEAMPSDIFFAHSSLLERAGSFVNRKTITALPILQTVDGDITSLISSNIISITDGQIVTSSDLFASGKLPAINIDLSVSRTGSSVQSRMMTKIAGEINKIFRSYKRHLKLAMLDYELNKETSILLHKGKLIDKMFLQKGFSLYSYRFVVLTTKLIS", "aa_hexdigest": "191f1297b78296469ae700fd94d6d26a", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A432FAQ0", "query_cov": 0.8853503184713376, "subject_cov": 0.8938906752411575, "identity": 0.525, "score": 251.0, "evalue": 1.05e-78, "valid": false, "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein", "uniref50_id": "UniRef50_A0A432FAQ0" }, "pscc": { "uniref50_id": "UniRef50_A0A432FAQ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432FAQ0" ], "product": "ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_684", "locus": "GCA013368035_00672" }, { "type": "cds", "sequence": "contig_1", "start": 389274, "stop": 389564, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAACACAACTAAAAATCCTCGTATTAAATCAATATTCAATTGCATAGCAACAATTGAGGGAGAATATAACTACCGCCAAAATCAACAATTTTATTTAGCAAGTAACCCAAATGTTAAATTCATAGTTATTTCCGCTACAACTAATAAGGCATTTATGTTGATTAATAGCGAACACGTATCTTTAGATATAAATAGTGAATTAATTGAATTAAATGAATCCCAAAATATAAATACTAGTCTTGATTATTTGGGCAAAATTATTGATATTAACGGTGGCATTATTTGA", "aa": "MNNTTKNPRIKSIFNCIATIEGEYNYRQNQQFYLASNPNVKFIVISATTNKAFMLINSEHVSLDINSELIELNESQNINTSLDYLGKIIDINGGII", "aa_hexdigest": "847cb441a179908963e96e9bf3c768b2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10856.16159999999, "isoelectric_point": 5.187547111511229 }, "id": "GLJLFKCOHC_685", "locus": "GCA013368035_00673" }, { "type": "cds", "sequence": "contig_1", "start": 389580, "stop": 389909, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTGGTATAAAACATAATTTTGACTTATTATCAAATTTAGCAAGTAATATTTCAATAAAAGCTAATAAAGAAGAAAAAGAAAATTTCAATAAAGCTTATTTAGAAGCAGTCAATGTTTTAAATAAAAATTATCTTGAACAAAAGAAGATTATTAAAAAACTAGGGACATCTTTTATAGTTATTTCTTTAATCATAATAATTACAAACATAATAATTTTTGCATTGTTAAAAAACTTAAAATTAAAGAAAAAGGTAATTATTATCAATAGTGTAATACTAGCGATTGCATTACTAATATTAATAATTGGAGGGCTATTATTTATATAG", "aa": "MSGIKHNFDLLSNLASNISIKANKEEKENFNKAYLEAVNVLNKNYLEQKKIIKKLGTSFIVISLIIIITNIIIFALLKNLKLKKKVIIINSVILAIALLILIIGGLLFI", "aa_hexdigest": "40d5d3327595f25768d062261b24a46e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12201.784299999981, "isoelectric_point": 9.948970603942868 }, "id": "GLJLFKCOHC_686", "locus": "GCA013368035_00674" }, { "type": "cds", "sequence": "contig_1", "start": 390252, "stop": 390827, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTGATTTAAAACCATTGGTTGATGGTGATAAAACAAGTCACTTAACAAAAGAACAAGAAAAAAATTTAATTCAATCATTGCCCCTTTTGGTTCCGTTTATATCAAGTAATAATTTGCCTTCATCATTAAGTCAAATTAAAAGCCAATTTGATTCATTAGATGCAAGCAAACGTCAAGAATTATTTTATTTCAATAACCCAATAAATACTCGTTATAAATATTCTGTTTTTTCATTTGATATTAATGGCAATGATATTATTAATTTAAAAGTACATATTTCAGATAATATAAATCCTAAGTTAGAAAGAACATATTTAATTGAAAAATATACTCCGTCACAAGACAAATATTTTAATCTCTTAAAAGAAACTGAAATAAAATCAATTTCAGAAATATTTAAGCCATTATATAAAGGTTTGGGCTTAGACGAAAAATTAGATTACAATAAGCTAAGAAATGGATATTTAAGAAAAGCATTGCTTGCAATGATTGACTCAGCGCTACAAATGTCATTTAAAGCTGATAAAGATTCATATAGTGCAATTAGCAATAAAATATTGCAAAAATATTGA", "aa": "MPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFSFDINGNDIINLKVHISDNINPKLERTYLIEKYTPSQDKYFNLLKETEIKSISEIFKPLYKGLGLDEKLDYNKLRNGYLRKALLAMIDSALQMSFKADKDSYSAISNKILQKY", "aa_hexdigest": "a1fe49a80fd3a88367be83e06cbb0b65", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22086.043200000004, "isoelectric_point": 9.001156425476076 }, "id": "GLJLFKCOHC_687", "locus": "GCA013368035_00675" }, { "type": "cds", "sequence": "contig_1", "start": 390936, "stop": 391631, "strand": "-", "frame": 3, "gene": null, "product": "MSC_0620 family F1-like ATPase-associated subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37", "UniRef:UniRef90_UPI0034D97E37" ], "nt": "ATGAATAAAAAGAAAATCTTATTATTATCATTTGCAACAGTTCCTTTATTATCTCCAATAGTAGTTTCGGCAGCGTCAAATAATGATCCAAATAATAAAACTGAGTTAGATCCAAATTTTGGTGAATTTGAAAGCGAAGCTAAAAAAACAATTTCTTCAACAATAGAAGATGGTTTAAAAGCAATAATCAATTATTTGCAAGTTCAACAACAAAGATTGCTAGAAAATAAAGAATTAGAATTTAAATTAAAAATTCAACAATTAATTTATTTAAAAAATTTAGAAACATTTCTAACAAAAAATAAAGAAGAAATTCAAAAAGATCCTAATAAGTATGGAATTTATTTAAATACTCCCCTTATATTAGGTAAAGAAAAAAACCACGATTTGCAGGATATTGATTACGAAGGCGATTTATTTAAATCAATAAAAACTGGTAAAACCGATCCACTAGATTATAAAAAAGCAATTTTGCCAAAAGGAAGTGTTAAAGAGGTTGCTAAAGATCAATTAAATACTATTAAAAAAGATAAGTATTCTCAATTTTTAAAGAAATATAAATCTGATTTCTTAAAAGAAGTTAATAAGTTATTTTTTAATGAAAAAGATGTTTTAAATATTGACAAAGATGTTGAATTAATTAGGGATAAAAACGGCGTATTTTCTACCACTTTGCCCAAAGGCTTTAAAAGTTGA", "aa": "MNKKKILLLSFATVPLLSPIVVSAASNNDPNNKTELDPNFGEFESEAKKTISSTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKDPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKTDPLDYKKAILPKGSVKEVAKDQLNTIKKDKYSQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKS", "aa_hexdigest": "72639f69ee5e4a1420f123ff4bef449f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 390633, "stop": 391631, "observations": { "insertion": [ 391112 ], "deletion": [ 391033 ], "mutation": [ 390937 ], "stop": [ 390937, 391060, 391084 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034D97E37", "query_cov": 1.0043290043290043, "subject_cov": 0.6609686609686609, "identity": 0.875, "score": 370.0, "evalue": 1.86e-126, "gene_start": 1, "gene_end": 231, "reference_start": 1, "reference_end": 232, "reference_sequence": "MNKKKILLLSFATVPLLSPIVVSAASNNDPNNKKPELDPNFGEFESEAKKTISSTIEDGLKAIINYLQVQQQRLLENKELEFKLKIQQLIYLKNLETFLTKNKEEIQKDPNKYGIYLNTPLILGKEKNHDLQDIDYEGDLFKSIKTGKTDPLDYKKAILPKGSVKEVAKDQLNAVKKDKYYQFLKKYKSDFLKEVNKLFFNEKDVLNIDKDVELIRDKNGVFSTTLPKGFKSWDEYFISKFKVRITKFDLKQNQETNEAEQKEEKNPDSLPDLKPLVDGDKTSHLTKEQEKNLIQSLPLLVPFISSNNLPSSLSQIKSQFDSLDASKRQELFYFNNPINTRYKYSVFFIWY" }, "paralog": false, "identity": 0.924924924924925, "score": 579.0, "evalue": 3.12e-211, "description": "Internal stop codon at 390937, 391060, 391084. Frameshift due to insertion around 391112. Frameshift due to deletion around 391033. Nonsense mutation around 390937.", "psc": { "uniref90_id": "UniRef90_UPI0034D97E37", "product": "MSC_0620 family F1-like ATPase-associated subunit", "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034D97E37", "UniRef:UniRef50_UPI0034D97E37" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034D97E37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D97E37" ], "product": "MSC_0620 family F1-like ATPase-associated subunit" } }, "id": "GLJLFKCOHC_688", "locus": "GCA013368035_00676" }, { "type": "cds", "sequence": "contig_1", "start": 391756, "stop": 391947, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAAAATTATTGATATTTCAAAACAAAACGAAACCTTCTTTGTTTTTTTAAAAAACTCAAATATTGTTGTAAACAATAACATAGCTACAATTTCAACATTAACCCAACCTCATTTTTATATAAAATCGCTCAACAAGGTCAATTTAGTTAAAACAATATCTGAACTTTCAAACGAGATTAAATTTTGA", "aa": "MLKIIDISKQNETFFVFLKNSNIVVNNNIATISTLTQPHFYIKSLNKVNLVKTISELSNEIKF", "aa_hexdigest": "edfaebffce1a82b1e3afec534e243af4", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7257.405499999999, "isoelectric_point": 9.523801612854005 }, "id": "GLJLFKCOHC_689", "locus": "GCA013368035_00677" }, { "type": "cds", "sequence": "contig_1", "start": 391990, "stop": 392091, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAACGTATATAAAATTGAATTACTTTTTTTAGAAAATAAGAAGCTAAATATTGAAAAAGGCTTAGTTTATATTAATGTCAATGAAGAAAATACATGA", "aa": "MNNVYKIELLFLENKKLNIEKGLVYINVNEENT", "aa_hexdigest": "c3ec65c564742151077d47bea6c19336", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3940.5179000000016, "isoelectric_point": 5.062103462219238 }, "id": "GLJLFKCOHC_690", "locus": "GCA013368035_00678" }, { "type": "cds", "sequence": "contig_1", "start": 392084, "stop": 392572, "strand": "-", "frame": 1, "gene": null, "product": "MSC_0622 family F1-like ATPase gamma subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95", "UniRef:UniRef90_D1J8A4" ], "nt": "TTGCAATCTTATGAAAATTCAAATATTGAAAATTTGGCAGAAAAATTAACCAAAATAGTAATGATCTTGTATGAATATGAAAATTACGAAGAAGTCAATTTTGTAGTTAACTCCAATAAAAATTTTAATCAATATTTTACAATTTTGCCAATGTCTAAATTCTCGTTTGAAAAATTTCCTTTAAATGAAAGCAATAATAATTTATATGACATAGATTTTAAAAAAGCAAAAATATATCCCAATATTAATGATTTTATTAATTCTCAAATTAATTGTTATTTATTAAACACTATAAATATGCTAATATCTGAATCTGCATTTTATAAAGCAAAAATAGGTTTAGTTTCAACTAATACAATTTTAAAAGATTTAGATGAAAGCATTCTTAAACTTAAAAAGCAAATATCAAGAGCAAAAAGTGAATTGCAAATTGAGGAACTAAATTTATTGACTAAAAAAGACCCTGATGAAGGTGATATTTATGAATAA", "aa": "MQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE", "aa_hexdigest": "78276d3a930eca3700b485055d598886", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 392084, "stop": 392872, "observations": { "mutation": [ 392725 ], "stop": [ 392725 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8A4", "query_cov": 1.0, "subject_cov": 0.5436241610738255, "identity": 0.9940000000000001, "score": 309.0, "evalue": 2.87e-104, "gene_start": 1, "gene_end": 162, "reference_start": 137, "reference_end": 298, "reference_sequence": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESRHRFNLFRKINRNILWIYLTEEQKYSTDSYSRYEKTILSNIKKSNNEFILIGKNAIKFGQDNNLQVLQSYENSNIENLAEKLTKIVMILYEYENYEEVNFVVNSNKNFNQYFTILPMSKFSFEKFPLNESNNNLYDIDFKKAKIYPNINDFINSQINCYLLNTINMLISESAFYKAKIGLVSTNTILKDLDESILKLKKQISRAKSELQIEELNLLTKKDPDEGDIYE" }, "paralog": false, "identity": 0.9961832061068703, "score": 512.0, "evalue": 6.7e-187, "description": "Internal stop codon at 392725. Nonsense mutation around 392725.", "psc": { "uniref90_id": "UniRef90_D1J8A4", "product": "MSC_0622 family F1-like ATPase gamma subunit", "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8A4", "UniRef:UniRef50_Q9PR95" ] }, "pscc": { "uniref50_id": "UniRef50_Q9PR95", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9PR95" ], "product": "Uncharacterized protein UU050" } }, "id": "GLJLFKCOHC_691", "locus": "GCA013368035_00679" }, { "type": "cds", "sequence": "contig_1", "start": 392723, "stop": 392980, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAACTAAAAGATTTAGAGAATAAACGCAAAAGTTTAAACAATATTTCATTAAAAGTAAATAATGAAAAAAATATTTATTTAATAAATATTATGAAACTTAACCAAAGATTAAGCTTTTTTAATAAAAATGCATTGGTATCAAGGCAAATAATTTTGCATTTAAAAGAAATCTACAATATTAAGAATGATATGTTGAGGAGAGAAAGTCGCCATAGGTTTAATTTATTTAGGAAAATAAATAGAAACATATTGTGA", "aa": "MQLKDLENKRKSLNNISLKVNNEKNIYLINIMKLNQRLSFFNKNALVSRQIILHLKEIYNIKNDMLRRESRHRFNLFRKINRNIL", "aa_hexdigest": "046d97bb3379b92a8896eac11c6d5c80", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10438.226399999985, "isoelectric_point": 11.068786430358884 }, "id": "GLJLFKCOHC_692", "locus": "GCA013368035_00680" }, { "type": "cds", "sequence": "contig_1", "start": 392982, "stop": 393485, "strand": "-", "frame": 3, "gene": null, "product": "DUF2714 domain-containing protein", "db_xrefs": [ "RefSeq:WP_175949886.1", "SO:0001217", "UniParc:UPI00158927EC", "UniRef:UniRef100_UPI00158927EC", "UniRef:UniRef50_D1J8A5", "UniRef:UniRef90_D1J8A5" ], "nt": "ATGAAAAAGAAAGACAATAATCCATTATTGAGTTATTTTGATGTATTTAAAGAATATAAAGAACACGAGTCCTCACAAGATTTAATTACTTTTGATGAAATGATATCAACAGTATTATTTAACAACAACCTAGGATTTAATTCTCCTATTTATTTAGATTTTATAAGCAAAATTGATTTAGCTTTTAAAAATAAATACGACATTTTGCTAAAGGATTTTAACATTACTTTTAATTTGAATTTGAAATTCTCACAAGAGGTATTGGTGCCTATAATTACAACAAAAAATTATTCTGCCAATAACGCAATTGATTTAACTGCTTCTAGCGATATAAAATACCAAGAATTTTTGAATTCATATAATGCTGAAATAAAAAAACTTTTGGCTCTTAAAAAATCAATTAAAATATTTCCACATATAATTTTATTTCATTCAGAATTAAAAAATGATTTAGTTTTGTTATTCAGTGAAAAAGTAGTAGCAGTTGTTACAAAGGAACAATAA", "aa": "MKKKDNNPLLSYFDVFKEYKEHESSQDLITFDEMISTVLFNNNLGFNSPIYLDFISKIDLAFKNKYDILLKDFNITFNLNLKFSQEVLVPIITTKNYSANNAIDLTASSDIKYQEFLNSYNAEIKKLLALKKSIKIFPHIILFHSELKNDLVLLFSEKVVAVVTKEQ", "aa_hexdigest": "cd5810bd5b2b05ef522c18b97d283dde", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158927EC", "ncbi_nrp_id": "WP_175949886.1", "uniref100_id": "UniRef100_UPI00158927EC", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158927EC", "RefSeq:WP_175949886.1", "UniRef:UniRef100_UPI00158927EC" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158927EC", "uniref90_id": "UniRef90_D1J8A5" }, "psc": { "uniref90_id": "UniRef90_D1J8A5", "product": "DUF2714 domain-containing protein", "uniref50_id": "UniRef50_D1J8A5" }, "pscc": { "uniref50_id": "UniRef50_D1J8A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8A5" ], "product": "DUF2714 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_693", "locus": "GCA013368035_00681" }, { "type": "cds", "sequence": "contig_1", "start": 393466, "stop": 393897, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTAGCAATTGTTCAAATAGCGCTTGCGTTAAGTTTGCTATTGACTACATTATTTAACACCAATAGCATTGTTTCTTTAATCAACGTTGCTGTAACAGCAACAACACAAATAGTAATATTATTTGTGTTTGCTAAACAAAACAAGAATATTTCATTGCTATTATTAATAATAATTAAACTAGCAATAATCTCTTCAATTTTAATCTTTGCAATTATTGGATTTAATTATATTTTAACTGCTGATGGCAATGAAAATTATTTATTTTCAATAATTAAAAGTAGAATGAATATTATTCAAATTATTTTACTTTTAAACTTTAGCATTGCATTTATTATGGCATTATCTTTATTGCTTAAATTTATTTTGGCAATTTATTATATAAGAAAATTAAATAAGGAGAATGAATATGAAAAAGAAAGACAATAA", "aa": "MNLAIVQIALALSLLLTTLFNTNSIVSLINVAVTATTQIVILFVFAKQNKNISLLLLIIIKLAIISSILIFAIIGFNYILTADGNENYLFSIIKSRMNIIQIILLLNFSIAFIMALSLLLKFILAIYYIRKLNKENEYEKERQ", "aa_hexdigest": "bc1bb912b49d686897dc3e72e78d6903", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16181.440299999986, "isoelectric_point": 9.646098136901855 }, "id": "GLJLFKCOHC_694", "locus": "GCA013368035_00682" }, { "type": "cds", "sequence": "contig_1", "start": 394069, "stop": 394293, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGTAGGCAATAAATTTTATGAAACAATTAGAGATATTTTAACAATTAAATCTGCTAGCAATTTTGTGATTATTGTATTAACATTTTTAGTTTTAATTACCTTTATAGTATTATCTAATATTAAATTCTTTGCTTTATTGATAAATAAAAAATATGATAAATTCTATTTAAAAAATCAATTAGTATTTTTATTAGTGTTATTTGGGGCTGTATTTATTTGA", "aa": "MFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKKYDKFYLKNQLVFLLVLFGAVFI", "aa_hexdigest": "e6dcd8d811ada762508a58acf77a9bbf", "start_type": "TTG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8644.464499999995, "isoelectric_point": 9.822999382019042 }, "id": "GLJLFKCOHC_695", "locus": "GCA013368035_00683" }, { "type": "cds", "sequence": "contig_1", "start": 394321, "stop": 394563, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCATTAACTTCTTATACATTATTTTTTAGCTATTTTAAAATTCTTCCAAGCAAATTATTTGCAATATCATTTATTTTGATTGCTTTATATATTATTAATTTTAGCTATGAAATATTTAACTTTTTAATTCAAAGAAAAACAAATCCATTGCTTTATAAAAATAAAAATATTTTAATAATTTCTTTAACATTTCAAGGGATTTTAACAATATTATTTGTTGCAATATCATTTGTTTGA", "aa": "MLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFV", "aa_hexdigest": "cd0216d165248626dfe7c9cb85b8c6b8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9370.249799999992, "isoelectric_point": 9.822999382019042 }, "id": "GLJLFKCOHC_696", "locus": "GCA013368035_00684" }, { "type": "cds", "sequence": "contig_1", "start": 394612, "stop": 394947, "strand": "-", "frame": 2, "gene": null, "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527", "UniRef:UniRef90_UPI001E41E527" ], "nt": "ATGAAACAAAAACAAATCGATTCGAATAATATAAATCAAAAGTTGATTTATGATGATGAATATTCAATAAAAAATCAGCAGAAAAACAACATTTTAAATAAGGTGTTGAAATACCTTTTGATTGCAATTAGTCTTGTATTTATTACAACTTTATTAATTTTTAGTGAAAAGACACTTTTTGCAAATAAAATGTTGCTTTCTAATTCACTAAATTCATTTTTTGACTTTTCAAGTCCAAGAAATCAACAATTAAACTTTTTAATAATTTATAGATTCTTAATTTTAGCCTTTGTCTTATTTTATAGTTTAGTAAAAGGTTTTGCTAATTTATATTGA", "aa": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNILNKVLKYLLIAISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFLILAFVLFYSLVKGFANLY", "aa_hexdigest": "df838a5a4388fdc43b6c8bf31c9c974f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 394309, "stop": 394947, "observations": { "mutation": [ 394323, 394614 ], "stop": [ 394323, 394614 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E41E527", "query_cov": 1.0, "subject_cov": 0.4036363636363636, "identity": 0.973, "score": 194.0, "evalue": 2.97e-60, "gene_start": 1, "gene_end": 111, "reference_start": 1, "reference_end": 111, "reference_sequence": "MKQKQIDSNNINQKLIYDDEYSIKNQQKNNILNKVLKYLLIVISLVFITTLLIFSEKTLFANKMLLSNSLNSFFDFSSPRNQQLNFLIIYRFFILSFVLFYSLVKGFANLYWHKVTIKKYIPFYFLYLLLSLTSYTLFFSYFKILPSKLFAISFILIALYIINFSYEIFNFLIQRKTNPLLYKNKNILIISLTFQGILTILFVAISFVWINLGINKDILFVGNKFYETIRDILTIKSASNFVIIVLTFLVLITFIVLSNIKFFALLINKNMINSI" }, "paralog": false, "identity": 0.9764150943396226, "score": 397.0, "evalue": 9.15e-143, "description": "Internal stop codon at 394323, 394614. Nonsense mutation around 394323, 394614.", "psc": { "uniref90_id": "UniRef90_UPI001E41E527", "product": "MSC_0624 family F1-like ATPase-associated membrane protein", "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E41E527", "UniRef:UniRef50_UPI001E41E527" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E41E527", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E41E527" ], "product": "MSC_0624 family F1-like ATPase-associated membrane protein" } }, "id": "GLJLFKCOHC_697", "locus": "GCA013368035_00685" }, { "type": "cds", "sequence": "contig_1", "start": 394963, "stop": 395310, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAGAAGACTTACTTTATATAATAACAGTGAAGCGTTCAACATATCAGGTGAAGAATTAAATGAAGCCGGTTTTAATAGACATATTGTTTCGGGCGAACCTGGTCAAAAAAGCAAGATTTTATTTAGTAATGGAAATTTAACAACAAAAGTAAGAATCACAGGAACTTCATTACTAAATTCAGAAGGAATTGACAACCTTTCGCGTTTATATATGTTTGCAGAACAATTGAAAAAAGAAATAATTGTTGATAATAATGCCTCAGCTTTAAAATCTCAATTAGAAAGTTTGGGATATAGTGTAAAGACCTCTTCTTCTTCTGATTCTGACGGAGATTTTATTTAA", "aa": "MIRRLTLYNNSEAFNISGEELNEAGFNRHIVSGEPGQKSKILFSNGNLTTKVRITGTSLLNSEGIDNLSRLYMFAEQLKKEIIVDNNASALKSQLESLGYSVKTSSSSDSDGDFI", "aa_hexdigest": "318a4628c65f12273f4aa70cdc21e3b0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12636.944099999986, "isoelectric_point": 5.366248893737793 }, "id": "GLJLFKCOHC_698", "locus": "GCA013368035_00686" }, { "type": "cds", "sequence": "contig_1", "start": 395695, "stop": 395880, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAAAATCTGACTCTAGCCAAAAATTTATGGATATTTTAAAGGCAATTCCTGATGAGATTCAACTACTAGAACTATTTTTCTCAGCGGGCTCTTCAAACACTTCTTCTTTAATTGCGTTAGAAAATAAACACATAAAGGAACTAGGAATTTATACTTTAGGAAATTCATTATTGGATAAATGA", "aa": "MGKSDSSQKFMDILKAIPDEIQLLELFFSAGSSNTSSLIALENKHIKELGIYTLGNSLLDK", "aa_hexdigest": "f763aab2bca3814fe56dea6fe263b2ba", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6701.625999999998, "isoelectric_point": 5.082394981384277 }, "id": "GLJLFKCOHC_699", "locus": "GCA013368035_00687" }, { "type": "cds", "sequence": "contig_1", "start": 396106, "stop": 396372, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCTCATTTAGATTTCTCTAAATTTACAAAGATTACTTCTAATGCTGAAAAGCAATTAGCTGAAGGCCTTGCATTAGATCAAGACAACGCCTATGTAAACGAAAAAGGCGAGCTAGATTCATATGCATATTCTCCAGCCAAAGGCTATAATGGTGTTGCAAATAGAATAATAAACGACAATAAAAATAGAAGAGTTTTTGGATATGATAGTATTGAAAATAGAAGCCCATATGGTCTTGCTAATGGCGACTATCCTGGTTGA", "aa": "MYSHLDFSKFTKITSNAEKQLAEGLALDQDNAYVNEKGELDSYAYSPAKGYNGVANRIINDNKNRRVFGYDSIENRSPYGLANGDYPG", "aa_hexdigest": "a74f8834f7055dc56c4ed59ec082d28e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9832.597499999996, "isoelectric_point": 5.229266929626463 }, "id": "GLJLFKCOHC_700", "locus": "GCA013368035_00688" }, { "type": "cds", "sequence": "contig_1", "start": 396580, "stop": 397251, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACTTATTAAAGAAAAGAAAAAACAAATTAATTTTAATGACGGCAGGTGTTATTATAGGAGCTTCAATAATTGCTGGGGCTATATTTTATAGTACAAGTTCGCAAAATTATTCTCGTTTTAATACCACCAGCTCAGCAACAGGAAATGATTTCATAAATAAAAGCAACCTAGATTATAAAAATGCGTTGCCCTCAATAAAGGATAACAACTTAAAAGAATTAGAAAAGCCACAACCAATTCCAACCCCTAAACCAGAGCTTGAACCAATCCCAGTTCCGCCGGCTCCAAAACCCGAACCCAAACCAGAACCAAAACCTCAACCTCAACCAACCCCTCTAGCTCCAAAACCAGAGCCAAAGCCAAAACCCGAACCTAAGCCTACTCCTCCTTCATCAAAACCTTCAACTAGAACAACTGAAATTATTCTAAACGGTGTCAAAGTTGAAGCGGAAGTTGAAGATCAAAAGAAACGTGAATATAATCAATATGATATCTTAAATCAACTAACTAATTTAAACCCATATATCAATGATAGTGCTCCAAAAATTAAAAAAGTTAAGGTTACTGAAGAATTGAAACAAGCAGCAGCCCAAGCGGCTACTCAACGTTTAAAAAGAGATGAAAAATACTTCAAAAAAGATCTTGAAGATTATGAAAAAAGTTGA", "aa": "MKNLLKKRKNKLILMTAGVIIGASIIAGAIFYSTSSQNYSRFNTTSSATGNDFINKSNLDYKNALPSIKDNNLKELEKPQPIPTPKPELEPIPVPPAPKPEPKPEPKPQPQPTPLAPKPEPKPKPEPKPTPPSSKPSTRTTEIILNGVKVEAEVEDQKKREYNQYDILNQLTNLNPYINDSAPKIKKVKVTEELKQAAAQAATQRLKRDEKYFKKDLEDYEKS", "aa_hexdigest": "4eaf8deb29a1fb7cae11808272110e55", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 24991.38130000001, "isoelectric_point": 9.52193202972412 }, "id": "GLJLFKCOHC_701", "locus": "GCA013368035_00689" }, { "type": "cds", "sequence": "contig_1", "start": 397282, "stop": 397758, "strand": "-", "frame": 2, "gene": null, "product": "MIP family Ig-specific serine endopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "nt": "GTGTTTTTAGCACAACCAGGTGGAATGAAGCAAGTTCAAGAAGAAAAAGACGGCCAAAAATATCAAAAATCAGTATTTTCATTCTATGAAAGTAGCGATGGCAAAACATATATGCGTATGGGGCTAAATTATATGCCTAAACATTTTGCTCCAGTTGGAGGCGCTTCTGGCACAAGCTTAAGAAATCAAAATAACGAACTAGTTGGAATATTTTATTTATCAAATATAGGCGCAAAAACCGGTTTAGCAGCTGCTTTTAGATCGGAAGGATTTGACTACAAAGGCTTGTATGGAAATTACAATTTGCCTCAATATGATTTAATTTATGGCGGCGGTAAAGACCAAAAAAATTCATTTAGACAAACATTAAAAGAACTATATAAAAATAATAGCGATTTTAGAACAGCATTATTCCCTAATGGCTTAGGTGATGATAAAGTTCCTAATGAATTTAAATTTATAGAAAATAAAAAATAA", "aa": "MFLAQPGGMKQVQEEKDGQKYQKSVFSFYESSDGKTYMRMGLNYMPKHFAPVGGASGTSLRNQNNELVGIFYLSNIGAKTGLAAAFRSEGFDYKGLYGNYNLPQYDLIYGGGKDQKNSFRQTLKELYKNNSDFRTALFPNGLGDDKVPNEFKFIENKK", "aa_hexdigest": "9a02a226e1bd59781014ba3ce1e1d27d", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021DFAD80", "query_cov": 0.8354430379746836, "subject_cov": 0.8048780487804879, "identity": 0.598, "score": 161.0, "evalue": 5.03e-48, "valid": false, "product": "MIP family Ig-specific serine endopeptidase", "uniref50_id": "UniRef50_UPI0021DFAD80" }, "pscc": { "uniref50_id": "UniRef50_UPI0021DFAD80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021DFAD80" ], "product": "MIP family Ig-specific serine endopeptidase" }, "genes": [], "id": "GLJLFKCOHC_702", "locus": "GCA013368035_00690" }, { "type": "cds", "sequence": "contig_1", "start": 398029, "stop": 398484, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01732.22" ], "nt": "ATGGATAGTGCAAAAATTAAAACAACATTGCGTTTGGCAAATCTTGATGAAAATACATATAGATTTTCATTTGTTGGTAAAGATTCTACTAATGTTTCTAATGGAATAAAGACAGTTTATACTGCAAGAGATTTCTTAAATTCAAAACCTTCTGATTATTTAATTAAATCGCAAAAAGAAAAGTATAAAGATGTTGAAGAATATGCTGATTTTGCAGTTTTTGAAGTTGATTTTGAAAAATTGACATTACAAAGCGTTTCTAAAAATGATCTAAGCGAAAATAGCGATATTACACAAAAATTTAGCAATTTAGATGCCCACGAATTAGCAAGAGCCTTAACAAGTAATTATGCTCAAAATAAAGAAAAACAAATTAAATTCTTGTCGAAATCATATTTATCAAATTACCAAAAAATTGATGTTCCTTTAAAGGCAAATAAAGAAAACAAAGAATGA", "aa": "MDSAKIKTTLRLANLDENTYRFSFVGKDSTNVSNGIKTVYTARDFLNSKPSDYLIKSQKEKYKDVEEYADFAVFEVDFEKLTLQSVSKNDLSENSDITQKFSNLDAHELARALTSNYAQNKEKQIKFLSKSYLSNYQKIDVPLKANKENKE", "aa_hexdigest": "17d85716bc95a8f2c8b1fb86361f8fa0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01732.22", "name": "Mycop_pep_DUF31", "length": 149, "aa_cov": 0.9271523178807947, "hmm_cov": 0.8322147651006712, "evalue": 8.271458198583351e-26, "score": 86.81053161621094, "start": 2, "stop": 141 } ], "seq_stats": { "molecular_weight": 17389.2111, "isoelectric_point": 7.777610969543457 }, "id": "GLJLFKCOHC_703", "locus": "GCA013368035_00691" }, { "type": "cds", "sequence": "contig_1", "start": 398605, "stop": 399213, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTTTAATATTACTATTAAAGACAAAAAGACAAACAAAACATTTATTGAAACAATAACTGTAGGAAAATTTAAAACAAATCCTTATGGTTTTGATGAAAATGGAACAATTCCTAATACAGGTTCTGACGCTTTAAGATTGAATAAAAGTGATTTGGAACAATATCTTGAAGGAAATCAAACAAAAAGATATGAGTTAGATAATAAAAAATATTTAGACATTTTAACTCGTCAACAAGAACAAAAATCAATAAAAGAAATCAGACCTAATTTGGAAGCTTCACAATCTAACATTCAAAAATTTGATGCTAAGGCAAAAGAAATTAATCAAGATTCATATGAATCAGCTGCAAGAAAAGGTTTTACATTGCCAAGCTACGATTCAAAAGGCAATGTAGAAGGCCTTGCTATTAATGAAACTCCAGGAGGTCAAGGGCCTTCACCTACAGATGCATTTGGTAGAGATCCATATAAAATTACAGGTTTAGCAAGAACATTGCCGAATGAACATTATCGTGACATTGCTTCACAAACATTTGGAATAAGATTCTTAACTCCTATTGAAGGCCAAGAAAGAAAATTTACTAATACAGCTGGAACAACTTGA", "aa": "MNFNITIKDKKTNKTFIETITVGKFKTNPYGFDENGTIPNTGSDALRLNKSDLEQYLEGNQTKRYELDNKKYLDILTRQQEQKSIKEIRPNLEASQSNIQKFDAKAKEINQDSYESAARKGFTLPSYDSKGNVEGLAINETPGGQGPSPTDAFGRDPYKITGLARTLPNEHYRDIASQTFGIRFLTPIEGQERKFTNTAGTT", "aa_hexdigest": "05a9aa5060bee2b5fb97bf8b85170585", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22726.96319999999, "isoelectric_point": 8.595972633361818 }, "id": "GLJLFKCOHC_704", "locus": "GCA013368035_00692" }, { "type": "cds", "sequence": "contig_1", "start": 399238, "stop": 399708, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAATCTAAATTAATAATATCATTATTAATAGCGGCAACATCACTTTCTAGTATGTCTGCTGTAGCTATTTCATGTAAAAATACAAATAAAAAGACTAATAATAATCAAAATGACGAAAAGAAGAATCCTGATGAAAAACCCCAAAATAATCAAGATTCAAAAGGCAACGACAACAAAAATAATAATTCTACAGATGAACTATCAGTTGAAGAACAAAAAACTACCGAATATAAGAAAATAATTTCTGAGGTTTCAAAATTAGCATTAAATGATCCAAGTCAATTATTTGATTTGAAATTCAAGGCTTTTTCAAATCAAAATTCACAAGATTTGCTTCCATCTCAAATTGTTAGAAATTTCAATCAAGGTGTTGAATTTAAACAAAAAAAATTTAATGATGAAATTGAAATTAAAAAAATAGTCAATGTTTTATACGTACAAACTTCAACCAATTCTGATGATTGA", "aa": "MKKSKLIISLLIAATSLSSMSAVAISCKNTNKKTNNNQNDEKKNPDEKPQNNQDSKGNDNKNNNSTDELSVEEQKTTEYKKIISEVSKLALNDPSQLFDLKFKAFSNQNSQDLLPSQIVRNFNQGVEFKQKKFNDEIEIKKIVNVLYVQTSTNSDD", "aa_hexdigest": "d7fce002e021b902a6f593b205601f5e", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17633.4641, "isoelectric_point": 8.525508689880368 }, "id": "GLJLFKCOHC_705", "locus": "GCA013368035_00693" }, { "type": "cds", "sequence": "contig_1", "start": 400022, "stop": 400408, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V8AMI9" ], "nt": "ATGTTGGATGTTTGCAATTTAGCTAAAGTTTTCCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAACGGGGTTGGAAAATCCACTTTTTTAAAAATATTAAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGACGATTATATTGCAACTGATGTTGTAATAATGGGGAATAAAGAACTTTATCAAATTAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAAAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGA", "aa": "MLDVCNLAKVFPDKKLFTNVNLKFLPGNVYGIIGANGVGKSTFLKILSGDIEASEGQIIKEKNARMSVLSQNQNEFDDYIATDVVIMGNKELYQINLEKTKIYEDPNATIQDYEKASHLEEKFGEMGG", "aa_hexdigest": "c70b676939e47c862fa354f4f78ad32e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0011CB74F5", "query_cov": 0.8359375, "subject_cov": 0.9727272727272728, "identity": 0.514, "score": 108.0, "evalue": 2.01e-28, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_V8AMI9" }, "pscc": { "uniref50_id": "UniRef50_V8AMI9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_V8AMI9" ], "product": "ABC transporter domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_706", "locus": "GCA013368035_00694" }, { "type": "cds", "sequence": "contig_1", "start": 400472, "stop": 400600, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATGTCGGATTTAAAAGCTAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCTGATATTTTAGTAATGGATGAACCAACAAACAGATTGGATCTAAAAACAATTAAGTGA", "aa": "MKMSDLKANEKVKVLLAKALFGNPDILVMDEPTNRLDLKTIK", "aa_hexdigest": "767e909f7a3a553f1973341b68c98a0f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4742.6662, "isoelectric_point": 9.22402362823486 }, "id": "GLJLFKCOHC_707", "locus": "GCA013368035_00695" }, { "type": "cds", "sequence": "contig_1", "start": 400616, "stop": 400741, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTATCCAAATATTGTTATTGTTGTTTCTCACGACAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGACATAGATTATTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGA", "aa": "MDYPNIVIVVSHDSDFLDAICTHIIDIDYSEAKLFIGNYSF", "aa_hexdigest": "459d1e9fe7647d18e60ab69fb0c47a61", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4695.236499999998, "isoelectric_point": 4.0500284194946286 }, "id": "GLJLFKCOHC_708", "locus": "GCA013368035_00696" }, { "type": "cds", "sequence": "contig_1", "start": 400769, "stop": 401158, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33", "PFAM:PF12848.13" ], "nt": "ATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCTACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCATCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCTGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGACACTTTATTTGAAAATGTAAGTTTTACAATCCACCCCGGAGAAAAAATGGTTATTATTGGCAATGACGACATTGCCAAAACTAGATTGCTTGAAATACTTATGGGTAAGCGTAAACCAACATCAGGAACAGTTGAATGA", "aa": "MQQKQNLKKEEIAQKLKDFIARFSANASKSKQATSRKKLLDKIVIDEIKPSSRKYPFIQFNLNRTPGKQILTVKNLTYVNDNGDTLFENVSFTIHPGEKMVIIGNDDIAKTRLLEILMGKRKPTSGTVE", "aa_hexdigest": "a837073c1b585038c050706acd779c73", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 41, "aa_cov": 0.3178294573643411, "hmm_cov": 1.0, "evalue": 6.738494263684968e-08, "score": 28.639638900756836, "start": 88, "stop": 128 }, { "id": "PF12848.13", "name": "ABC_tran_Xtn", "length": 59, "aa_cov": 0.4573643410852713, "hmm_cov": 0.9661016949152542, "evalue": 4.236246280297967e-13, "score": 44.65205001831055, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 14693.900499999978, "isoelectric_point": 9.908742332458495 }, "id": "GLJLFKCOHC_709", "locus": "GCA013368035_00697" }, { "type": "cds", "sequence": "contig_1", "start": 401297, "stop": 401635, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAGAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATCTTCGACCAACCTCTTGACCACCTTGATAGTGAATCAATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGAGCAATGATAAAAGAATGTGCCTCAGTTATTTTAGAAATAAAACCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAA", "aa": "MRAFLGRMLFSNDSVFKNVNVTSGGEKVRLMMAKLMLEESNFLIFDQPLDHLDSESIDSLIEAIKNYKSSCIFTTYNRAMIKECASVILEIKPTSSYLFYGTLEEYEEAMGY", "aa_hexdigest": "09499d814b522967d8a94203f3f165be", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12834.661299999987, "isoelectric_point": 4.819230461120605 }, "id": "GLJLFKCOHC_710", "locus": "GCA013368035_00698" }, { "type": "cds", "sequence": "contig_1", "start": 401680, "stop": 401859, "strand": "-", "frame": 2, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B9AR10", "UniRef:UniRef90_UPI000DFBAFCD" ], "nt": "ATGTTACAAAATTTTGTTATTCCAATCCCTCACATTTCCATTCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGATCAACGTAGAAAACAATACGAATATTATAGAAATAAGCTGCTAAACTTTAAAAAGTAA", "aa": "MLQNFVIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRRKQYEYYRNKLLNFKK", "aa_hexdigest": "5c2944c1559097e6033dbb70c17c4032", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000DFBAFCD", "query_cov": 0.9322033898305084, "subject_cov": 0.8870967741935484, "identity": 0.927, "score": 102.0, "evalue": 1.34e-27, "valid": true, "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_A0A5B9AR10" }, "pscc": { "uniref50_id": "UniRef50_A0A5B9AR10", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A5B9AR10" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "GLJLFKCOHC_711", "locus": "GCA013368035_00699" }, { "type": "cds", "sequence": "contig_1", "start": 402038, "stop": 402259, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTCCATTTCCTGATATTAATATTGACATTTCATCAAAGGCATATTCATTTATTAATTTTGGTCTTTCATCGCATGTTCAAAACACATATTTTCCATTTGGAGTTTCTGCATTTGCGTTTAATTTTCCGGTTTTTATTTCTCTAAAAATTTTTGATATAGGTAAGTATTCAACTATATTGACAATTTTGTCTCAAAGTTCATTTAAAAGCAGTAAATAA", "aa": "MLPFPDINIDISSKAYSFINFGLSSHVQNTYFPFGVSAFAFNFPVFISLKIFDIGKYSTILTILSQSSFKSSK", "aa_hexdigest": "a5e3de845bcf683d7b8c40a450de1722", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8183.338899999997, "isoelectric_point": 9.158652687072756 }, "id": "GLJLFKCOHC_712", "locus": "GCA013368035_00700" }, { "type": "cds", "sequence": "contig_1", "start": 402263, "stop": 402586, "strand": "-", "frame": 1, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6" ], "nt": "GTGTGTGGAATATTGAGTGTTCTAAATAAATCAAAATTAAATGTGAAATATTTAAGCACTTGCTTATCTATGCAGACAAAAAAATTTGTAAACAAGGCAAGTGGAAATCCTAAACTTATGAGTAATATTATGGAAAATATTGAAATCCCAATCCCTCATATTTCCATTCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGTATTAGGTAA", "aa": "MCGILSVLNKSKLNVKYLSTCLSMQTKKFVNKASGNPKLMSNIMENIEIPIPHISIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGIR", "aa_hexdigest": "7ffd2b8ccbcbf5c88c6117abbebab8e4", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_Q9PR47", "query_cov": 0.8691588785046729, "subject_cov": 0.9207920792079208, "identity": 0.774, "score": 141.0, "evalue": 1.15e-41, "valid": false, "product": "Type I restriction enzyme S protein", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0AAC9T2H6", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0AAC9T2H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAC9T2H6" ], "product": "Type I restriction modification DNA specificity domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_713", "locus": "GCA013368035_00701" }, { "type": "cds", "sequence": "contig_1", "start": 402650, "stop": 402799, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01420.25" ], "nt": "TTGTGTGAAATAAAGCGAGGAAAAGTTTACTCTAAGGAATTCATAAAATTAAATAAAGGTGAATATCCTGTTTATTCATCTCAAAGTTTAAACGATGGAATTTTAGGAAAAATTGACAAATATGATTTTGATGGTGAACATGTAACATGA", "aa": "MCEIKRGKVYSKEFIKLNKGEYPVYSSQSLNDGILGKIDKYDFDGEHVT", "aa_hexdigest": "1fb01301a923ae17a5e344b927bc7bcc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01420.25", "name": "Methylase_S", "length": 53, "aa_cov": 1.0, "hmm_cov": 0.9811320754716981, "evalue": 3.833732907695347e-09, "score": 32.3620719909668, "start": 1, "stop": 49 } ], "seq_stats": { "molecular_weight": 5644.346700000001, "isoelectric_point": 6.537245750427247 }, "id": "GLJLFKCOHC_714", "locus": "GCA013368035_00702" }, { "type": "cds", "sequence": "contig_1", "start": 402873, "stop": 403412, "strand": "-", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00131480C8", "UniRef:UniRef90_UPI00131480C8" ], "nt": "TTGTCATTTAAGCATGTTTCCGCAAATGAACTTAAGCTCTATAAAAAAGAAGAAACGGGAAACGTAAAATTATTATCAACAGGTCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCATCTGGTGGCGTGCCATCAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTATTTTTTAATTGCTAACAAAGAAAACATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATTGAATTGCAAATTCCACTCCCCCCTCTTGAAATCCAAAATAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACCAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGTATTAAGTAA", "aa": "MSFKHVSANELKLYKKEETGNVKLLSTGQYDGFTSYVENDKNINYGEIISIPSGGVPSIKYYKGYFIDSLNILLSSKDVKKYSLKFLYYFLIANKENIEQNFRGSSIKHPDMENIIELQIPLPPLEIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGGIK", "aa_hexdigest": "393427d504c5af719fb9c7fa3b3980a3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131480C8", "query_cov": 0.9832402234636871, "subject_cov": 0.8036529680365296, "identity": 0.972, "score": 342.0, "evalue": 3.07e-118, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00131480C8", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI00131480C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00131480C8" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "GLJLFKCOHC_715", "locus": "GCA013368035_00703" }, { "type": "cds", "sequence": "contig_1", "start": 403473, "stop": 404201, "strand": "-", "frame": 3, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A454CAF7", "UniRef:UniRef90_UPI0013145F86" ], "nt": "ATGAACAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAAAAAATTGGAGAAATTTCTACATTGTTTTCGGGTATGAAGGGAGTATCTAAAAAATGGAGTGAAGAAGGGAATTGCAAATTTATAGATTATATGAATTGCTATAAAAATTTTAGAATTGATGTATCTTTACTAAAAAATGCCACAGTAAAATCATTAAATCAAAATATATTGCAAAAAAACGATATCTTGTTTACGGCTTCATCAGAAACCCCTGACGAATGTTGCATATCTTCGATTATAGATGATTATATTGAGGATAAAATATTTATGGATGATCATTTATTTGCTATAAGAATTTTAAAAGATTTTTTAAAAGAGATTAATCCTATATATTTAAATTTTTATTTTCATTGTGTTGATTTTAGAAAAAAGGTTAATAAAATTGCTAAAGGAATGACTAGATTTTATATTCCTAAATCCGATTTTTTAAATATTCAAATTCCGCTTCCCCCTCTTGAAATTCAAAATAAAATTGTTAATATTTTAGACAAGTTCACCGAATTGACCACCGAATTGACCACCGAATTGACCACCGAATTGACCTATAGAGATAAACAATATAATTACTATAGAAATAAATTACTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAATCAACAATGCAATAATAGTATAGTTGAATATAAGAGGTTATGA", "aa": "MNKIEKLIKELCPNGVEYKKIGEISTLFSGMKGVSKKWSEEGNCKFIDYMNCYKNFRIDVSLLKNATVKSLNQNILQKNDILFTASSETPDECCISSIIDDYIEDKIFMDDHLFAIRILKDFLKEINPIYLNFYFHCVDFRKKVNKIAKGMTRFYIPKSDFLNIQIPLPPLEIQNKIVNILDKFTELTTELTTELTTELTYRDKQYNYYRNKLLDFDNNKELLNKIMNNQQCNNSIVEYKRL", "aa_hexdigest": "8b7a4add7e335b444320654b292603f4", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 403170, "stop": 404201, "observations": { "mutation": [ 403475 ], "stop": [ 403475 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0013145F86", "query_cov": 1.0330578512396693, "subject_cov": 0.5555555555555556, "identity": 0.968, "score": 456.0, "evalue": 1.53e-158, "gene_start": 1, "gene_end": 242, "reference_start": 1, "reference_end": 250, "reference_sequence": "MNKIEKLIKELCPNGVEYKKIGEISTLFSGMKGVSKKWSEEGNCKFIDYMNCYKNFRIDVSLLKNATVKSLNQNILQKNDILFTASSETPDECCISSIIDDYIEDKIFMDDHLFAIRILKDFLKEINPIYLNFYFHCVDFRKKVNKIAKGMTRFYIPKSDFLNIQIPLPPLEIQNKIVNILDKFTELTTELTTELTTELTTELTTELTYRDKQYNYYRNKLLDFDNNKELLNKIMNNQQCNNSIVEYKRLWEIVDFDKKFKGVDKNKQQNILSFKHVSANELKLYKKEETGNVKLLSTGQYDGFTSYVENDKNINYGEIISIPSGGVPSIKYYKGYFIDSLNILLSSKDVKKYSLKFLYYFLIANKENIEQNFRGSSIKHPDMENIIELQISLPPLEIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGGIR" }, "paralog": false, "identity": 0.9743589743589743, "score": 659.0, "evalue": 2.85e-241, "description": "Internal stop codon at 403475. Nonsense mutation around 403475.", "psc": { "uniref90_id": "UniRef90_UPI0013145F86", "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A454CAF7", "kegg_orthology_id": "K01154", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0013145F86", "EC:3.1.21.3", "UniRef:UniRef50_A0A454CAF7", "KEGG:K01154" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF7" ], "product": "Type I restriction modification DNA specificity domain-containing protein" } }, "id": "GLJLFKCOHC_716", "locus": "GCA013368035_00704" }, { "type": "cds", "sequence": "contig_1", "start": 404204, "stop": 404668, "strand": "-", "frame": 1, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAAGCAACTTGTTTTTTGGAACAAGTATTTCAACGTGCATAATGGTATTGAAAAAATCAAAAAATGACAACAATATCTTATTTATTGACGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAGCTAACTAGTCAAAATATTAACAATATTATTGATTATTATGGCCAAAGAAAAGACATTTCTTATATTTCAAAATTAGCCAGTGTTGAAGAAATTAAGTCCAATTCATATAATTTATCAGTTAATAGTTACGTTGAAAAACAAGACACTTCAGAAAAAATAGATATTAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGCGAAATTGACAAAATAGTTGCTGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKNDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQRKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "2a717d9b45574f4cce9bfeab2fc65bae", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.66, "score": 200.0, "evalue": 2.11e-63, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 404204, "stop": 404968, "observations": { "mutation": [ 404716, 404827 ], "stop": [ 404716, 404827 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.987, "score": 279.0, "evalue": 4.28e-92, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.984251968503937, "score": 486.0, "evalue": 3.64e-176, "description": "Internal stop codon at 404716, 404827. Nonsense mutation around 404716, 404827.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "GLJLFKCOHC_717", "locus": "GCA013368035_00705" }, { "type": "cds", "sequence": "contig_1", "start": 404825, "stop": 405652, "strand": "-", "frame": 1, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAACGTCAAGCAGGAATTGAAGATTTTGATTATACAACTTTATCAGATGAAGAAGCATTGACGGGCAAAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAAAAATGCTTCAACAAACAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAAGGACAACAAAGTGAAAATGATTTTTCAGGTCTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGCAATTATCAAGATAATTCAATAGATGCCTTTGGTGATGCCTATGAATATTTGATGTCAATGTATGCATCAAATGCCGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCTGAATTACTTACTAAAATAGCAGTTTTTAATAAGAAAAAAGTTAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTCTGCTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAATATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATATAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGACAAATTCAATATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATCGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQRQAGIEDFDYTTLSDEEALTGKDDLINEKGFFILPSELFINVVKNASTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "a4553c1e9cf4635b87e870e1fb8a2367", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.655, "score": 365.0, "evalue": 4.45e-124, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "GLJLFKCOHC_718", "locus": "GCA013368035_00706" }, { "type": "cds", "sequence": "contig_1", "start": 405962, "stop": 406516, "strand": "-", "frame": 1, "gene": "yajL", "product": "DJ-1/PfpI domain-containing protein", "db_xrefs": [ "COG:COG0693", "COG:V", "RefSeq:WP_036433265.1", "SO:0001217", "UniParc:UPI000513FD89", "UniRef:UniRef100_UPI000513FD89", "UniRef:UniRef50_D1J8B4", "UniRef:UniRef90_D1J8B4" ], "nt": "ATGAATTTATTAGTAATTGTTGAAGAAGGATTTGAAGATACTGAATTAACAGGAACATTAGATTTATTTCATAGAGCAAATAATTTATTTAATAAGACCACATTTTATAATCCTCTAGGAATTGAAGCAACTGGCGCACATAAAATTGTAAAATTGCAAACAACTTCAAATATAAATGTTGATGAATATGATGTTTTATTTATTCCTGGAGGACCTGCTGCTAAAAGTATTAGAAAGAACCAAACATTTTTAAACTTAATAGCTAAGTTTAAAAATCAAAACAAATTAATTATAGCAATATGCGATGCTCCCAATGTTTTATATGAAAATAAGTTTATTGATAGTAAAACTAAATTTAGTTCATTTCCAATAAACGACAGTGTAAAAGAAATATCTAATCGTAGTAAAGAATTAGTATCAATAAGTCCAGATAAGAAATTAATAACAGGAAAATGTGCGGCTGCTGTTTTTGATTTTGCATTAGCTGTTATTGAAGTTTTAGGTGGCAATCAACTTGCTAAATTAGTTCTTCAAGCAATAAAACCTATTGATTAA", "aa": "MNLLVIVEEGFEDTELTGTLDLFHRANNLFNKTTFYNPLGIEATGAHKIVKLQTTSNINVDEYDVLFIPGGPAAKSIRKNQTFLNLIAKFKNQNKLIIAICDAPNVLYENKFIDSKTKFSSFPINDSVKEISNRSKELVSISPDKKLITGKCAAAVFDFALAVIEVLGGNQLAKLVLQAIKPID", "aa_hexdigest": "ca68752eda1cc60dcc0211335c93a6eb", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI000513FD89", "ncbi_nrp_id": "WP_036433265.1", "uniref100_id": "UniRef100_UPI000513FD89", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513FD89", "RefSeq:WP_036433265.1", "UniRef:UniRef100_UPI000513FD89" ] }, "ips": { "uniref100_id": "UniRef100_UPI000513FD89", "uniref90_id": "UniRef90_D1J8B4" }, "psc": { "uniref90_id": "UniRef90_D1J8B4", "gene": "YajL", "product": "DJ-1/PfpI domain-containing protein", "uniref50_id": "UniRef50_D1J8B4", "cog_id": "COG0693", "cog_category": "V" }, "pscc": { "uniref50_id": "UniRef50_D1J8B4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B4" ], "product": "DJ-1/PfpI domain-containing protein" }, "genes": [ "yajL" ], "id": "GLJLFKCOHC_719", "locus": "GCA013368035_00707" }, { "type": "cds", "sequence": "contig_1", "start": 406669, "stop": 406848, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "TTGCCTGGTTTATATATTAATATTCATAAATTAACAATTCATATGAATTCAAATCCTAAAACAATTGAAATGGATTTCAGCTTAAAATATTCAAAACCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKPKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "bb4655e076c067ee84989a364753b6ae", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 406669, "stop": 407016, "observations": { "mutation": [ 406857 ], "stop": [ 406857 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 1.0, "subject_cov": 0.5086206896551724, "identity": 0.966, "score": 118.0, "evalue": 2.44e-33, "gene_start": 1, "gene_end": 59, "reference_start": 58, "reference_end": 116, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9051724137931034, "score": 211.0, "evalue": 2.04e-72, "description": "Internal stop codon at 406857. Nonsense mutation around 406857.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7228.2817, "isoelectric_point": 7.9542543411254885 }, "id": "GLJLFKCOHC_720", "locus": "GCA013368035_00708" }, { "type": "cds", "sequence": "contig_1", "start": 406855, "stop": 407034, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI001939F8FF" ], "nt": "TTGATTCCTAAAAAAGTTTTACTCGAAGCAAAATTTATTGATAATGAAAAAGTTGATTTAAAAGAAGCATTCTTTAAATATAATTCATTATATCGAAACTCTAAAACTAAAATTTATGCAAATTTAAAAAGCGGCGATATCACAATTAAAAACTTTGAATTAGGTGCTGCAAAAGATTGA", "aa": "MIPKKVLLEAKFIDNEKVDLKEAFFKYNSLYRNSKTKIYANLKSGDITIKNFELGAAKD", "aa_hexdigest": "d4e3c57745ec4d710eb940b5cc39dd1d", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 406669, "stop": 407091, "observations": { "mutation": [ 406857, 407076, 407088 ], "stop": [ 406857, 407076, 407088 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939F8FF", "query_cov": 1.0, "subject_cov": 0.42142857142857143, "identity": 0.9490000000000001, "score": 111.0, "evalue": 2.69e-30, "gene_start": 1, "gene_end": 59, "reference_start": 20, "reference_end": 78, "reference_sequence": "YWILDWFTLEDKEFNCIFFLIPKKVLLEAKFIDNKNVDLKEAFFKYNSLYRNSKTKIYANLKSGDITIKNFELGAAKDWKEMPGLYINIHKLTIHMNSDPKTIEMNFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9357142857142857, "score": 265.0, "evalue": 2.44e-93, "description": "Internal stop codon at 406857, 407076, 407088. Nonsense mutation around 406857, 407076, 407088.", "psc": { "uniref90_id": "UniRef90_UPI001939F8FF", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939F8FF", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6813.868300000002, "isoelectric_point": 9.435931205749512 }, "id": "GLJLFKCOHC_721", "locus": "GCA013368035_00709" }, { "type": "cds", "sequence": "contig_1", "start": 407155, "stop": 407325, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000DDC6D33", "UniRef:UniRef50_UPI00131471F2", "UniRef:UniRef90_UPI000DDC6D33", "UniRef:UniRef90_UPI00131471F2" ], "nt": "TTGCCAATTTTGCCATTATGTGTTACATCTTGCTATAATGAACAAAAACAACATAAAAAAGTTTTAAACATTATTGGAAATAGTAATCCACATTTTTATATAGAAAATTTATTCAGTGCCAAATCATATAATTCAGTATTGCAAAGCAATAAAAGTATAGCGGGTCCTTGA", "aa": "MPILPLCVTSCYNEQKQHKKVLNIIGNSNPHFYIENLFSAKSYNSVLQSNKSIAGP", "aa_hexdigest": "70e6362cf0bf28f2d1240c57d2172310", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00131471F2", "query_cov": 0.9464285714285714, "subject_cov": 0.9137931034482759, "identity": 0.9620000000000001, "score": 108.0, "evalue": 3.84e-30, "valid": true, "uniref50_id": "UniRef50_UPI00131471F2" }, "pscc": { "uniref50_id": "UniRef50_UPI00131471F2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00131471F2" ] }, "genes": [], "truncated": "both", "pseudogene": { "start": 407074, "stop": 407376, "observations": { "mutation": [ 407088, 407100, 407157, 407358 ], "stop": [ 407088, 407100, 407157, 407358 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000DDC6D33", "query_cov": 1.0, "subject_cov": 0.5544554455445545, "identity": 0.946, "score": 111.0, "evalue": 1.09e-30, "gene_start": 1, "gene_end": 56, "reference_start": 18, "reference_end": 73, "reference_sequence": "MMKKNKWKLLTIFGISTLPILPLCVTSCYNEQKQHKKALNIIKNSNPHFYIENLFSAKSYNSVLQSNKSIAGPWRNDFLEIAIGPKSKWFEYWKDYWIVDD" }, "paralog": false, "identity": 0.92, "score": 182.0, "evalue": 3.8e-61, "description": "Internal stop codon at 407088, 407100, 407157, 407358. Nonsense mutation around 407088, 407100, 407157, 407358.", "psc": { "uniref90_id": "UniRef90_UPI000DDC6D33", "uniref50_id": "UniRef50_UPI000DDC6D33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000DDC6D33", "UniRef:UniRef50_UPI000DDC6D33" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000DDC6D33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000DDC6D33" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6280.171400000001, "isoelectric_point": 9.100115394592287 }, "id": "GLJLFKCOHC_722", "locus": "GCA013368035_00710" }, { "type": "cds", "sequence": "contig_1", "start": 407526, "stop": 407648, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCCGGTGCATAAATTAGATGATAAGAATAGTGAACCCAGGTTGCGCTTTTACATTAAACAATTAACATTAGATTGCAATCTAAACAAAAAAAGACTTGAACTTGATGTTCATTTGTGA", "aa": "MAPVHKLDDKNSEPRLRFYIKQLTLDCNLNKKRLELDVHL", "aa_hexdigest": "d6aae8907188a7a9841541535ed99b59", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4805.582899999999, "isoelectric_point": 8.978850364685059 }, "id": "GLJLFKCOHC_723", "locus": "GCA013368035_00711" }, { "type": "cds", "sequence": "contig_1", "start": 407661, "stop": 408074, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EA444", "UniRef:UniRef90_UPI001E2EA444" ], "nt": "TTGCCATTATTTGTAACCTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGCAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAAAGAACAAGAATCAGTTTTAAATAACGACGCAACTATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCATATTCCATTTTGTTTTTAATTCCTAAAAAGGTATTATTAGAAACAGGATTTATTGCAAGCGAAGATTTTAATTTAGCAGACTTTTATAGTAAAAACCTTAATTTCGTGTTTAATGAACAGCCTAGAAAAATTTCAACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILKEQESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFIASEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKI", "aa_hexdigest": "9d0f7e8c892b3cf001cf0f70684bcc43", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 407598, "stop": 408131, "observations": { "mutation": [ 407663, 408116 ], "stop": [ 407663, 408116 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2EA444", "query_cov": 1.0, "subject_cov": 0.7611111111111111, "identity": 0.978, "score": 268.0, "evalue": 2.6e-90, "gene_start": 1, "gene_end": 137, "reference_start": 20, "reference_end": 156, "reference_sequence": "MKKNKWKLLTIFGISALPILPLLVTSCNNRETELKNAETIIGNKEIVFNMVDILKEQESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSKNLNFVFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNSEPRLLLH" }, "paralog": false, "identity": 0.9774011299435028, "score": 347.0, "evalue": 9.91e-124, "description": "Internal stop codon at 407663, 408116. Nonsense mutation around 407663, 408116.", "psc": { "uniref90_id": "UniRef90_UPI001E2EA444", "uniref50_id": "UniRef50_UPI001E2EA444", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2EA444", "UniRef:UniRef50_UPI001E2EA444" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2EA444", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2EA444" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15986.210499999994, "isoelectric_point": 7.665954399108887 }, "id": "GLJLFKCOHC_724", "locus": "GCA013368035_00712" }, { "type": "cds", "sequence": "contig_1", "start": 408131, "stop": 408370, "strand": "-", "frame": 1, "gene": null, "product": "Peptidase C51 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_A0A6A8Q0U9" ], "nt": "ATGCCTGATCCGATGGGAAGAGGAAAAGTTGGTCATGCAATTGTTGCTTATGGAATGTATGACAATGGAAATAAACTTCTTTGCCATTATGGTTGGGGAGGATATTCTCAAGTTATTGTTTCTTCGAGTCTTTCTGGCCAATTGTTCTTATTAGGTATGAAACCAAATGGCGAAATTAAAAAAACAAGGAAGCATTTTATTAAAAACGGAATTAAATATTCGGGAACGGATTTTAAATAA", "aa": "MPDPMGRGKVGHAIVAYGMYDNGNKLLCHYGWGGYSQVIVSSSLSGQLFLLGMKPNGEIKKTRKHFIKNGIKYSGTDFK", "aa_hexdigest": "bab9233b7d061f7d36f03a093ddfa95b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A6A8Q0U9", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.9490000000000001, "score": 160.0, "evalue": 3.15e-50, "valid": true, "product": "Peptidase C51 domain-containing protein", "uniref50_id": "UniRef50_A0A6A8Q0U9" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_725", "locus": "GCA013368035_00713" }, { "type": "cds", "sequence": "contig_1", "start": 408542, "stop": 408724, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTTATTATATGCTGAGTTTTTTCAAAATTATGGAGTCTTTTCAGATGACCAAGTGAAAAAATACTTTGTAGATTATCAGGATTCATCGGATTATTCAACATTTAAAAAATATGGCTATCCATATTCTCCTGCTATTTCTGATGAATTTGTTACAGATTTATGGACAAAACACGGATGA", "aa": "MLLLYAEFFQNYGVFSDDQVKKYFVDYQDSSDYSTFKKYGYPYSPAISDEFVTDLWTKHG", "aa_hexdigest": "4bb3a9178a0874eeb5bba70cbcd99aa9", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7159.808000000001, "isoelectric_point": 4.465635108947754 }, "id": "GLJLFKCOHC_726", "locus": "GCA013368035_00714" }, { "type": "cds", "sequence": "contig_1", "start": 408914, "stop": 409519, "strand": "-", "frame": 1, "gene": null, "product": "Peptidase C39-like domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U2", "UniRef:UniRef90_A0A454C9U2" ], "nt": "ATGTTTGAATTAGTTGAATATATGCAAAAAATTGACAATTTCAATAAATTTGTTCAAAATAATGCAAAAAAAATAAATGAACTAAAAAAATATAATATTTCTGATGTAAAAGCTTATTTAAAGTTTTTGGAATTGGAAATATTTAAATACAATAGGAAAATTGATTATCAAATAAAAGATATAGAAATTATTTTTGACAATTTTGGTAATCCATATATTTATGTTAATTTTAATCCTTACGGATATATGGTTGTTTCGTTAATAAATAATGAAAGTGTCATCATTGATCCTTTGAATAAAAATGAAAATTTTAACAATAGCGATTTCCATAAAAAATATTTTTTAGACTTAGATTCCTTAAGAATTCAGCCAAAAAATAACTCTTCTCAAATTCGTTCTTATCAAGAAAATTTTGTTTTAAAAAATTTCAAAGGTAAATATTACGACATTAGTGATAATTTAAATAATAAAAAATTAATAGAAGAAAAACAAAATTTAAAGTTTTCACTAAAAAAGTCTATTTCTTATAGAAATTCAAGCGAGGCTCATGAAATTAATACTGATGTTGAAATGATACATGCAGAAAAAGAAGTTCCGTATTCTTGA", "aa": "MFELVEYMQKIDNFNKFVQNNAKKINELKKYNISDVKAYLKFLELEIFKYNRKIDYQIKDIEIIFDNFGNPYIYVNFNPYGYMVVSLINNESVIIDPLNKNENFNNSDFHKKYFLDLDSLRIQPKNNSSQIRSYQENFVLKNFKGKYYDISDNLNNKKLIEEKQNLKFSLKKSISYRNSSEAHEINTDVEMIHAEKEVPYS", "aa_hexdigest": "9711709749debcc4082cadf3f1e47f1f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 408611, "stop": 409519, "observations": { "mutation": [ 408847, 408913, 408916 ], "stop": [ 408847, 408913, 408916 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454C9U2", "query_cov": 1.0, "subject_cov": 0.4331896551724138, "identity": 0.98, "score": 382.0, "evalue": 3.23e-130, "gene_start": 1, "gene_end": 201, "reference_start": 1, "reference_end": 201, "reference_sequence": "MFELIEYMQKIDNFNKFVQNNAKKINELKKYNISDVKAYLKFLELEIFKYNRKIDYQIKDMEIIFDNFGNPYIYVNFNPYGYMVVSLINNESVIIDPLNKNENFNSSDFHKKYFLDLDFLRIQPKNNSSQIRSYQENFVLKNFKGKYYDISDNLNNKKLIEEKQNLKFSLKKSISYRNSSEAHEINTDVEMIHAEKEVPYSWWFKLAIKDKNFGYQSYSTINARWEKEKEEAKLRGDEGEAKFIQDKIDKYKCSDIHQGLCHYVALGMLLLYAEFFQNYGVFSDDQVKKYFVDYQDSSDYSTFKKYGYPYSPAISDEFVTDLWTKHGWGAIVTTSSYLKNVAYDFLKKPDLPNFWPVYVYRRSAGWIKPWKWIRDGYPCLIFGTYMPDPMGRGKVGHAIVAYGMYDNGNKLLCHYGWGGYSQVIVSSSLSGQLFLLGMKPNGKVYKTRKHFIKNGIKYSGTDFK" }, "paralog": false, "identity": 0.9473684210526315, "score": 575.0, "evalue": 1.96e-208, "description": "Internal stop codon at 408847, 408913, 408916. Nonsense mutation around 408847, 408913, 408916.", "psc": { "uniref90_id": "UniRef90_A0A454C9U2", "product": "Peptidase C39-like domain-containing protein", "uniref50_id": "UniRef50_A0A454C9U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454C9U2", "UniRef:UniRef50_A0A454C9U2" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454C9U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454C9U2" ], "product": "Peptidase C39-like domain-containing protein" } }, "id": "GLJLFKCOHC_727", "locus": "GCA013368035_00715" }, { "type": "cds", "sequence": "contig_1", "start": 409784, "stop": 409963, "strand": "-", "frame": 1, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAACAAAAAATATAATTTTGAATTCAAACTTGGCGCAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCGCGAAGTTGCAAAAGTTTCTTTTAAAGCAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKATIKALEKIDNKLE", "aa_hexdigest": "a45c24f2c698fc254ac1fe87ce423f5a", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 409784, "stop": 410092, "observations": { "mutation": [ 409966, 410077 ], "stop": [ 409966, 410077 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 0.966, "score": 108.0, "evalue": 1.05e-29, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9411764705882353, "score": 180.0, "evalue": 1.74e-60, "description": "Internal stop codon at 409966, 410077. Nonsense mutation around 409966, 410077.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "GLJLFKCOHC_728", "locus": "GCA013368035_00716" }, { "type": "cds", "sequence": "contig_1", "start": 410075, "stop": 410359, "strand": "-", "frame": 1, "gene": null, "product": "ribonuclease HIII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1B727", "UniRef:UniRef90_UPI0034A1B727" ], "nt": "GTGATAATAAATTTTTTGAGGTTATTTCTTGAAAAAATAATTGAAATTTTTAATCAAATTGAAAATTTGGTTTATTATCAAAAAACTTGCCTTAGTCAAAGCGGTTATAATAAATTGATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAATCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAGTGTTACAAAAGCATTTCAAAAAATTGCAAGCAATTTCTTGA", "aa": "MIINFLRLFLEKIIEIFNQIENLVYYQKTCLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEVLQKHFKKLQAIS", "aa_hexdigest": "5967dca649ef80679decf9c8d6050d05", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 409811, "stop": 410415, "observations": { "deletion": [ 410382 ], "mutation": [ 410400 ], "stop": [ 409965, 410076, 410400 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A1B727", "query_cov": 0.9893617021276596, "subject_cov": 0.4603960396039604, "identity": 0.9570000000000001, "score": 166.0, "evalue": 1.37e-50, "gene_start": 2, "gene_end": 94, "reference_start": 22, "reference_end": 114, "reference_sequence": "VKKYKNSSVKNIPLEFFKFKVITNFLRLFHEKIIEIFNQIENLVYYQKTYLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEMLQKHFKKLQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKA" }, "paralog": false, "identity": 0.9502487562189055, "score": 356.0, "evalue": 9.72e-128, "description": "Internal stop codon at 409965, 410076, 410400. Frameshift due to deletion around 410382. Nonsense mutation around 410400.", "psc": { "uniref90_id": "UniRef90_UPI0034A1B727", "product": "ribonuclease HIII", "uniref50_id": "UniRef50_UPI0034A1B727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A1B727", "UniRef:UniRef50_UPI0034A1B727" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A1B727", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A1B727" ], "product": "ribonuclease HIII" } }, "id": "GLJLFKCOHC_729", "locus": "GCA013368035_00717" }, { "type": "cds", "sequence": "contig_1", "start": 410422, "stop": 410601, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF", "UniRef:UniRef90_UPI0015F0C8A9" ], "nt": "TTGCCTGGTTTATATATTAATATTCACAAATTAACAATTCATATGAATTCAAATCCTAAAACAATTGAAATGGATTTCAGCTTAAAATATTCAAAATCTAAAGAACATCAACAAAAGATTGAAGGTTTTGAAGATGACTATATTTTTGAATATAAATATATATTCAAAGAAAAAAAGTAA", "aa": "MPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK", "aa_hexdigest": "084e7b801b313560ac8c22a03bd1e288", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 410422, "stop": 410772, "observations": { "mutation": [ 410610 ], "stop": [ 410610 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "query_cov": 1.0, "subject_cov": 0.5086206896551724, "identity": 0.983, "score": 120.0, "evalue": 6.01e-34, "gene_start": 1, "gene_end": 59, "reference_start": 58, "reference_end": 116, "reference_sequence": "MFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSNPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKK" }, "paralog": false, "identity": 0.9396551724137931, "score": 226.0, "evalue": 2.45e-78, "description": "Internal stop codon at 410610. Nonsense mutation around 410610.", "psc": { "uniref90_id": "UniRef90_UPI0015F0C8A9", "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0015F0C8A9", "UniRef:UniRef50_UPI001939F8FF" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939F8FF", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F8FF" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7218.2438, "isoelectric_point": 7.9542543411254885 }, "id": "GLJLFKCOHC_730", "locus": "GCA013368035_00718" }, { "type": "cds", "sequence": "contig_1", "start": 410608, "stop": 410838, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E608893", "UniRef:UniRef90_UPI001E608893" ], "nt": "TTGCTTCTAGAAGATAAAGAATCCAATTCAAAAAAATGTATTTTTTTCTTGATTCCTAGAAAAGTTTTACTTGAAGCAAAATTTATTGATAATGAAAATGTTGATTTAAAAGAAGCATTCTTTAAATATAATTCAGGATATTGGAACTATAAAAAAACTAAAATTTATGCAAATTTGAAAAGTGGCGATATCACAATTAAAAACTTTGAATTAGGTGCTGCAAAAGATTGA", "aa": "MLLEDKESNSKKCIFFLIPRKVLLEAKFIDNENVDLKEAFFKYNSGYWNYKKTKIYANLKSGDITIKNFELGAAKD", "aa_hexdigest": "81e0a69238cb4da417f1d920816f4e01", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 410416, "stop": 410952, "observations": { "mutation": [ 410424, 410610, 410853, 410865, 410877, 410922 ], "stop": [ 410424, 410610, 410853, 410865, 410877, 410922 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E608893", "query_cov": 1.0, "subject_cov": 0.42696629213483145, "identity": 0.895, "score": 141.0, "evalue": 2.35e-41, "gene_start": 1, "gene_end": 76, "reference_start": 39, "reference_end": 114, "reference_sequence": "MQSNKSIAGPWHNDFLEIAIGPKSKWFEYWKDYWIVDRLVLEDKESNSKKFIFFLIPRKVLFEAKFIDNENVDLKEAFFKYNSGYSNFKKTKVYANLKSGEITIKNFELGAAKDWKELPGLYINIHKLTIHMNSDPKTIEMDFSLKYSKSKEHQQKIEGFEDDYIFEYKYIFKEKSKC" }, "paralog": false, "identity": 0.8876404494382022, "score": 315.0, "evalue": 2.01e-112, "description": "Internal stop codon at 410424, 410610, 410853, 410865, 410877, 410922. Nonsense mutation around 410424, 410610, 410853, 410865, 410877, 410922.", "psc": { "uniref90_id": "UniRef90_UPI001E608893", "uniref50_id": "UniRef50_UPI001E608893", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E608893", "UniRef:UniRef50_UPI001E608893" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E608893", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E608893" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8902.208499999993, "isoelectric_point": 8.99045467376709 }, "id": "GLJLFKCOHC_731", "locus": "GCA013368035_00719" }, { "type": "cds", "sequence": "contig_1", "start": 411429, "stop": 411854, "strand": "-", "frame": 3, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6", "UniRef:UniRef90_UPI00158BFF58" ], "nt": "TTGCCAATATTGCCCTTATTTGTAACCTCTTGCAATAATAGAGAAACTGAATTAAAAAATGCCGAAACAATTATTGGTAATAAAGAAATAGTTTTTAATATGGTTGATATTTTAAATAGCGAAGAAGAATCAGTTTTAAATAACGACGCAACTATTTCTGGCCCATGGCGTAATGATTTTTTTAAAATTGTAATAACATCAAAAAAGAATTTTGATAAATATGAAAAAGATTATTGTGTTTTTGGAAAGATAGATTTTTATGCATATTCCATTTTGTTTTTAATTCCTAAAAAGGTATTATTAGAAACAGGATTTATTGCAAGCGAAGATTTTAATTTAGCAGACTTTTATAGTGAAAAACTTAATGTTGCGTTTAATGAACAGCCTAGAAAAATTTCAACAAATTTAAAAAATAAAAAAATATGA", "aa": "MPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFIASEDFNLADFYSEKLNVAFNEQPRKISTNLKNKKI", "aa_hexdigest": "6bca0f17b69482de7871b06672294750", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 411256, "stop": 411902, "observations": { "deletion": [ 411320 ], "mutation": [ 411431, 411887 ], "stop": [ 411263, 411305, 411311, 411431, 411887 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158BFF58", "query_cov": 1.0, "subject_cov": 0.6558139534883721, "identity": 0.986, "score": 276.0, "evalue": 4.49e-93, "gene_start": 1, "gene_end": 141, "reference_start": 17, "reference_end": 157, "reference_sequence": "MKKNKWKLLTIFGISALPILPLFVTSCNNRETELKNAETIIGNKEIVFNMVDILNSEEESVLNNDATISGPWRNDFFKIVITSKKNFDKYEKDYCVFGKIDFYAYSILFLIPKKVLLETGFISSEDFNLADFYSEKLNVAFNEQPRKISTNLKNKKIWIYKYMAPVHKLDDKNNKPELRFFYIKQLMLDCSLNKKKTWTWCSFVIYSTPKKRIWF" }, "paralog": false, "identity": 0.958139534883721, "score": 404.0, "evalue": 1.23e-145, "description": "Internal stop codon at 411263, 411305, 411311, 411431, 411887. Frameshift due to deletion around 411320. Nonsense mutation around 411431, 411887.", "psc": { "uniref90_id": "UniRef90_UPI00158BFF58", "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158BFF58", "UniRef:UniRef50_D1J8B6" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8B6" ], "product": "Lipoprotein" } }, "id": "GLJLFKCOHC_732", "locus": "GCA013368035_00720" }, { "type": "cds", "sequence": "contig_1", "start": 411902, "stop": 412141, "strand": "-", "frame": 1, "gene": null, "product": "putative cysteine peptidase", "db_xrefs": [ "RefSeq:WP_175949895.1", "SO:0001217", "UniParc:UPI00158A7EA2", "UniRef:UniRef100_UPI00158A7EA2", "UniRef:UniRef50_A0A6A8Q0U9", "UniRef:UniRef90_D1J8B7" ], "nt": "ATGCCTAATCCAATAAAAAAAGGAAAAATTGGTCATGCAATTGTTGCTTATGGAATGTATGACAATGGAAATAAACCTCTTTACCATTATGGATGGGGAGGATATTCTCAAGTAATTGTTTCTTCAAGTCTTTCTGGCGAATTATTTTTATTGGGCATGAAACCAAATGGAAAGGTTTATAAAACAAGGAAACATTTTATCAAAAAGGGAATTAAATATTCGGGAAAGGATTTTAAATAA", "aa": "MPNPIKKGKIGHAIVAYGMYDNGNKPLYHYGWGGYSQVIVSSSLSGELFLLGMKPNGKVYKTRKHFIKKGIKYSGKDFK", "aa_hexdigest": "658f0a2957513117ea8ef7fd0286cba7", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158A7EA2", "ncbi_nrp_id": "WP_175949895.1", "uniref100_id": "UniRef100_UPI00158A7EA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158A7EA2", "RefSeq:WP_175949895.1", "UniRef:UniRef100_UPI00158A7EA2" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158A7EA2", "product": "putative cysteine peptidase", "uniref90_id": "UniRef90_D1J8B7" }, "psc": { "uniref90_id": "UniRef90_D1J8B7", "uniref50_id": "UniRef50_A0A6A8Q0U9" }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q0U9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q0U9" ], "product": "Peptidase C51 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_733", "locus": "GCA013368035_00721" }, { "type": "cds", "sequence": "contig_1", "start": 412549, "stop": 412728, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53", "UniRef:UniRef90_UPI0023AACE53" ], "nt": "ATGCAACAACAAAACAAAAAATATAATTTTGAATTCAAACTTGGTGCAAGCAATAAAATTATTGATTTGGCAGCAAATTTTATTTTGAAATATGGTCAAAAGACATTGCGCGAGGTTGCAAAAGTTTCTTTTAAAACAACAATAAAAGCATTAGAAAAAATAGATAATAAATTAGAATAA", "aa": "MQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLREVAKVSFKTTIKALEKIDNKLE", "aa_hexdigest": "0084eb09148121a8e6683e2b4ad655e1", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 412549, "stop": 412857, "observations": { "mutation": [ 412731, 412842 ], "stop": [ 412731, 412842 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0023AACE53", "query_cov": 1.0, "subject_cov": 0.5784313725490197, "identity": 0.983, "score": 110.0, "evalue": 1.81e-30, "gene_start": 1, "gene_end": 59, "reference_start": 44, "reference_end": 102, "reference_sequence": "MQAISWLDFKKPQNEIIIETKGEEKSLAVAAASIISRKFLIDWMQQQNKKYNFEFKLGASNKIIDLAANFILKYGQKTLHEVAKVSFKTTIKALEKIDNKLE" }, "paralog": false, "identity": 0.9607843137254902, "score": 185.0, "evalue": 2.66e-62, "description": "Internal stop codon at 412731, 412842. Nonsense mutation around 412731, 412842.", "psc": { "uniref90_id": "UniRef90_UPI0023AACE53", "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0023AACE53", "UniRef:UniRef50_UPI0023AACE53" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0023AACE53", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023AACE53" ] } }, "id": "GLJLFKCOHC_734", "locus": "GCA013368035_00722" }, { "type": "cds", "sequence": "contig_1", "start": 412840, "stop": 413247, "strand": "-", "frame": 2, "gene": null, "product": "Ribonuclease HII", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B", "UniRef:UniRef90_UPI001F51E35B" ], "nt": "TTGAATAAAAATTTAGATGAATTAATTGGCGTTGATGAAACGGGAGTAGGTGATTATTTCAGTCCCGTAATTTCAGTTGCCTGTTATATACCAAAAGATAACATTGAAAAACTAAAACAAATTGGTGTTAAAGACAGCAAAAAACTTTCTGATAAAAGAATAATTGAAATTTTTAATCAAATTGAAAATTTAGTTTATTATCAAAAAACTTGCCTTAGTCAAAGTGGTTATAATAAATTAATTGATGCAGGTATTAATAACAACGAAATAAAAACATTAATTCATAGTAATTCAATAAATATATTAACAAAAAAATTAAATCAGAACATTGATGTTTTTATTGATCAATATACAACTTCATTAGAAGTGTTACAAAAGCATTTCAAAAAATTGCAAGCAATTTCTTGA", "aa": "MNKNLDELIGVDETGVGDYFSPVISVACYIPKDNIEKLKQIGVKDSKKLSDKRIIEIFNQIENLVYYQKTCLSQSGYNKLIDAGINNNEIKTLIHSNSINILTKKLNQNIDVFIDQYTTSLEVLQKHFKKLQAIS", "aa_hexdigest": "40df4bed03888e8dbebe34192f5fb909", "start_type": "TTG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_UPI001F51E35B", "query_cov": 0.9259259259259259, "subject_cov": 0.9057971014492754, "identity": 0.992, "score": 245.0, "evalue": 8.13e-82, "valid": true, "product": "Ribonuclease HII", "uniref50_id": "UniRef50_UPI001F51E35B" }, "pscc": { "uniref50_id": "UniRef50_UPI001F51E35B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F51E35B" ] }, "genes": [], "id": "GLJLFKCOHC_735", "locus": "GCA013368035_00723" }, { "type": "cds", "sequence": "contig_1", "start": 413240, "stop": 413548, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGATATTACAACATTGACATTGATGATACTGTTGCAATGGGAGATTCATATAATGATTTAAGCATGTATAATGTTGCTAATGTTTGTGTATCACCTGCGAATGCTGAACCCTTAATTAAAAAGATGTCAACAGTTGTTATGAAACAAACAAATAAAGAAGGCGCAGTTGGATACTTTATTGATCAATTCCTAAAAAACCCAGAAAAATACATTAAATTAGGTAAAAAACAAAAGGCCGAACAAAAAAATAATATTAAAACTGTAGGTGCAGATAACTTTTTTAGTAAAGAAGAAGAAATTGAATAA", "aa": "MRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE", "aa_hexdigest": "c8525aacea873a6f3ab319125237b291", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 60, "aa_cov": 0.5882352941176471, "hmm_cov": 0.9833333333333333, "evalue": 3.0645218178367167e-13, "score": 45.427085876464844, "start": 2, "stop": 61 } ], "seq_stats": { "molecular_weight": 11611.095899999991, "isoelectric_point": 5.506129646301268 }, "id": "GLJLFKCOHC_736", "locus": "GCA013368035_00724" }, { "type": "cds", "sequence": "contig_1", "start": 413627, "stop": 414022, "strand": "-", "frame": 1, "gene": "cof", "product": "Putative phosphatase in upp 3'region", "db_xrefs": [ "COG:COG0561", "COG:HR", "EC:3.1.3.-", "GO:0000287", "GO:0005829", "GO:0016791", "KEGG:K21064", "SO:0001217", "UniRef:UniRef50_P43051", "UniRef:UniRef90_P43051" ], "nt": "TTGCCTGTGTATGAAAAACTTGGACTTAATGCAATTGTAGGTAATTACAATGGTGCTCACATCCACAACCCTGCTGACCCATTCTTTATACCTGCTATTACATATTTGGACTTAAATGAAGTTCTTTATATTTTAGGCGATGAAAAAGTAAAAAAAGAAATTACTAATTATGCAATAGAAGGTCCTGACTGGGTTCAATTAATGCATAGAGATCCTAACCTTGAAAGAGTGTTTGGATTTAACCAAGCAACAAAATTTAGAGAATGTATTAACCTAGAAAAAATTCCACTAAAACCAACCGGAATTGTATTTGATGTTAAACCTGATACAGATGTTTTGGAATTATTGACATATTTAAAAAGACGTTATGGAGATTTAGGAGAATTTTCTTCTTGA", "aa": "MPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSS", "aa_hexdigest": "c77529f2e3482b857b9e30296c47109d", "start_type": "TTG", "rbs_motif": "AGxAG", "genes": [ "cof" ], "truncated": "both", "pseudogene": { "start": 413324, "stop": 414202, "observations": { "mutation": [ 413629, 414034 ], "stop": [ 413629, 414034 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43051", "query_cov": 1.0, "subject_cov": 0.409375, "identity": 0.992, "score": 275.0, "evalue": 2.73e-91, "gene_start": 1, "gene_end": 131, "reference_start": 61, "reference_end": 191, "reference_sequence": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRPWRSTLPVYEKLGLNAIVGNYNGAHIHNPADPFFIPAITYLDLNEVLYILGDEKVKKEITNYAIEGPDWVQLMHRDPNLERVFGFNQATKFRECINLEKIPLKPTGIVFDVKPDTDVLELLTYLKRRYGDLGEFSSWSKGEGLSPVFDITSIGIDKGKVISLIMRYYNIDIDDTVAMGDSYNDLSMYNVANVCVSPANAEPLIKKMSTVVMKQTNKEGAVGYFIDQFLKNPEKYIKLGKKQKAEQKNNIKTVGADNFFSKEEEIE" }, "paralog": false, "identity": 0.9931506849315068, "score": 580.0, "evalue": 6.94e-214, "description": "Internal stop codon at 413629, 414034. Nonsense mutation around 413629, 414034.", "psc": { "uniref90_id": "UniRef90_P43051", "gene": "Cof", "product": "Putative phosphatase in upp 3'region", "ec_ids": [ "3.1.3.-" ], "uniref50_id": "UniRef50_P43051", "cog_id": "COG0561", "cog_category": "HR", "kegg_orthology_id": "K21064", "go_ids": [ "GO:0005829", "GO:0000287", "GO:0016791" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43051", "EC:3.1.3.-", "UniRef:UniRef50_P43051", "COG:COG0561", "COG:HR", "KEGG:K21064", "GO:0005829", "GO:0000287", "GO:0016791" ] }, "pscc": { "uniref50_id": "UniRef50_P43051", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43051" ], "product": "Putative phosphatase in upp 3'region" } }, "id": "GLJLFKCOHC_737", "locus": "GCA013368035_00725" }, { "type": "cds", "sequence": "contig_1", "start": 414032, "stop": 414202, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGGCATACAACAAAGAATCAAAACAAAGAAGATTTTTATTTGCTATTGATCTAGATGGAACACTATTAGCAGATTCAGCCAATGGCACAGTTCATCCAAAAACAGAAGAAGCAATTAAAAAAGCTGTTGCCCAAGGACACATTGTTTCAATAATTACAGGAAGACCTTGA", "aa": "MAYNKESKQRRFLFAIDLDGTLLADSANGTVHPKTEEAIKKAVAQGHIVSIITGRP", "aa_hexdigest": "b4070446689c7714536aa9e25714399e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 43, "aa_cov": 0.7678571428571429, "hmm_cov": 0.9534883720930233, "evalue": 7.0509376895012865e-12, "score": 40.96463394165039, "start": 14, "stop": 56 } ], "seq_stats": { "molecular_weight": 6108.936900000001, "isoelectric_point": 9.30106334686279 }, "id": "GLJLFKCOHC_738", "locus": "GCA013368035_00726" }, { "type": "cds", "sequence": "contig_1", "start": 414385, "stop": 415014, "strand": "-", "frame": 2, "gene": "upp", "product": "uracil phosphoribosyltransferase", "db_xrefs": [ "COG:COG0035", "COG:F", "EC:2.4.2.9", "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206", "RefSeq:WP_020002490.1", "SO:0001217", "UniParc:UPI0003662176", "UniRef:UniRef100_A0A2K9YTA2", "UniRef:UniRef50_Q98QP6", "UniRef:UniRef90_P43049" ], "nt": "ATGGTAAAAATTTTTCAGCATCCCTTAATTAACGCTAAATTAACAACGATGCGTTCAAAAGAAACATCTTACAAAGATTTTAGAGATAATTTGAACGAGATTGCTTCGTTGATGGTATATGAAACATTGAGGGACTATCAAACTAAGAAAATATCAATAACAACACCAATGAATGTTAAATATGAAGGCGAAACTCTTGATAGAGAAATTGTAATCATTCCTATTTTACGTGCGGGGCTTGGAATGTTGAATGGTATTATGGATTTGGTTCCCCAAGCTAGAGTTGGTCATATTGGAATGTATAGAAACGAAGAAACAAACGAAGTTGTTGAATATTTCTTTAAAATTCCAGAAGTACCACATGATAGCTATATTATTATTGTTGACCCTATGCTTGCAACCGGCTCATCTGCATGCGATGCAATAGCAAAATTGGATAAGTTGGGCTTTAGCAATATTAAATTAGTATGTTTAGTAGGCGTTCAACAAGGTATTGATAAAGTAACAAAACAATTTCCTAATGTTGATATATATTTAGCTTCAAAAGATGAAAAATTGAATGAACATAATTATATATTACCTGGTTTGGGAGATGCTGGTGACCGTATATTTGGAACAAAAATAAAATAA", "aa": "MVKIFQHPLINAKLTTMRSKETSYKDFRDNLNEIASLMVYETLRDYQTKKISITTPMNVKYEGETLDREIVIIPILRAGLGMLNGIMDLVPQARVGHIGMYRNEETNEVVEYFFKIPEVPHDSYIIIVDPMLATGSSACDAIAKLDKLGFSNIKLVCLVGVQQGIDKVTKQFPNVDIYLASKDEKLNEHNYILPGLGDAGDRIFGTKIK", "aa_hexdigest": "3e720a22535f25de2c7be65caa1488af", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0003662176", "ncbi_nrp_id": "WP_020002490.1", "uniref100_id": "UniRef100_A0A2K9YTA2", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003662176", "RefSeq:WP_020002490.1", "UniRef:UniRef100_A0A2K9YTA2" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTA2", "uniref90_id": "UniRef90_P43049" }, "psc": { "uniref90_id": "UniRef90_P43049", "gene": "upp", "product": "uracil phosphoribosyltransferase", "ec_ids": [ "2.4.2.9" ], "uniref50_id": "UniRef50_Q98QP6", "cog_id": "COG0035", "cog_category": "F", "go_ids": [ "GO:0000287", "GO:0004845", "GO:0005525", "GO:0006223", "GO:0009116", "GO:0044206" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP6" ], "product": "Uracil phosphoribosyltransferase" }, "genes": [ "upp" ], "id": "GLJLFKCOHC_739", "locus": "GCA013368035_00727" }, { "type": "cds", "sequence": "contig_1", "start": 415042, "stop": 415704, "strand": "-", "frame": 2, "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "db_xrefs": [ "COG:COG0274", "COG:F", "EC:4.1.2.4", "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052", "KEGG:K01619", "RefSeq:WP_020002489.1", "SO:0001217", "UniParc:UPI000378BCA6", "UniRef:UniRef100_A0A2K9YTE4", "UniRef:UniRef50_Q98QP7", "UniRef:UniRef90_P43048" ], "nt": "ATGACAGAATTAAACAAATATATTGACCACACAAATTTATCACCTTCTGCAACAAGCAAAGACATTGATAAATTAATTCAAGAAGCAATTAAATACGATTTTAAATCAGTATGTATTGCACCATCATATGTTAAGTATGCTAAAGAAGCATTAAAAAACAGTGATGTGCTTGTTTGCACAGTTATTGGTTTTCCACTAGGCTATAATGCAACAAGTGTTAAAGTATATGAAACAAAAATTGCTGTTGAACATGGCGCTGACGAAATTGATATGGTAATCAATGTTGGAAGATTTAAAGATGGACAATATGAATACGTATTGAATGAAATCAAAGCAATAAAAGAAGCATGCAATGGCAAAACTTTAAAAGTTATAGTTGAAACAGCATTGTTAACAAAAGCTGAATTAATAAAAATTACTGAATTAGTAATGCAATCTGGTGCCGATTTTATTAAAACATCAACCGGATTTTCTTATAGAGGAGCTTCATTTGAAGACATTCAAACTATGAAAGAAACTTGTGGTGACAAGCTATTAATTAAAGCATCAGGTGGTATTAAAAATCTAGCAGATGCCCAAGAAATGATTAGATTAGGCGCAAACCGTTTGGGTATGTCTAAATCAGTTCCAGTTATGGAAGAATTATTTAAATTAGAAAAATAA", "aa": "MTELNKYIDHTNLSPSATSKDIDKLIQEAIKYDFKSVCIAPSYVKYAKEALKNSDVLVCTVIGFPLGYNATSVKVYETKIAVEHGADEIDMVINVGRFKDGQYEYVLNEIKAIKEACNGKTLKVIVETALLTKAELIKITELVMQSGADFIKTSTGFSYRGASFEDIQTMKETCGDKLLIKASGGIKNLADAQEMIRLGANRLGMSKSVPVMEELFKLEK", "aa_hexdigest": "58846acd5aac329a1c0a28ee84221e78", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000378BCA6", "ncbi_nrp_id": "WP_020002489.1", "uniref100_id": "UniRef100_A0A2K9YTE4", "db_xrefs": [ "SO:0001217", "UniParc:UPI000378BCA6", "RefSeq:WP_020002489.1", "UniRef:UniRef100_A0A2K9YTE4" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTE4", "uniref90_id": "UniRef90_P43048" }, "psc": { "uniref90_id": "UniRef90_P43048", "gene": "deoC", "product": "deoxyribose-phosphate aldolase", "ec_ids": [ "4.1.2.4" ], "uniref50_id": "UniRef50_Q98QP7", "cog_id": "COG0274", "cog_category": "F", "kegg_orthology_id": "K01619", "go_ids": [ "GO:0004139", "GO:0005737", "GO:0006018", "GO:0009264", "GO:0016052" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QP7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QP7" ], "product": "Deoxyribose-phosphate aldolase" }, "genes": [ "deoC" ], "id": "GLJLFKCOHC_740", "locus": "GCA013368035_00728" }, { "type": "cds", "sequence": "contig_1", "start": 415731, "stop": 415955, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07831.19" ], "nt": "ATGAAATTGGGAGCAGGTAGATCTAAAAAAGAAGATTCTATCGACTACGAAGCAGGAATTTATTTGAATAAGAGTTCAAATGAATATGTAAATAAAGGAGATGTATTATTTACATTGTATTCATCAAAGCCTATAAATCCAGAACTTCAAAAAGAATTGTTGTCTGCAATAGAATTTAGCGAAAGCAAGCATGACATTCAAACGGTTTTTGCTAAATTAATGTAA", "aa": "MKLGAGRSKKEDSIDYEAGIYLNKSSNEYVNKGDVLFTLYSSKPINPELQKELLSAIEFSESKHDIQTVFAKLM", "aa_hexdigest": "5fc7271d4064027fce5b036a85fdb1be", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07831.19", "name": "PYNP_C", "length": 60, "aa_cov": 0.7972972972972973, "hmm_cov": 1.0, "evalue": 6.999571918989033e-22, "score": 72.3613052368164, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8359.406099999997, "isoelectric_point": 5.74621753692627 }, "id": "GLJLFKCOHC_741", "locus": "GCA013368035_00729" }, { "type": "cds", "sequence": "contig_1", "start": 416094, "stop": 416705, "strand": "-", "frame": 3, "gene": null, "product": "Pyrimidine-nucleoside phosphorylase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "nt": "ATGTCAGGAAGAGGGCTTGGACATACAGGAGGAACACTTGATAAATTAGAATCAATTGACGGTTATAATTTTTCAGTGACTGACCAACAATTTAGAGATTTAGTTGAAAAGCATGGTATTGCAATAGTTGGACAAAATCAAAAACTTGTGCCTGCAGATAAAAAAATCTATGCATTACGTGATGTAACGGGTACCGTTCAATCAATTCCATTGATCGCCTCATCAATTATGTCTAAAAAACTTGCAACTGGTTCAAACTGCATTTTATTGGATGTTAAATGTGGCAATGGCGCATTTATGAAAGATATTAATGAAGCAAAAAAACTTGGAAAATTGATGATTGAAATCGGCAAGAAATTAAATAGAAAGATTGCCGTTGAAATAACCAATATGCAACAACCTTTAGGAAAAACTATTGGCAATAAAATTGAAGTTTTAGAAGCAATAGATACATTAAATGGACACGGACCTAAAGATTTTACAGAAATTATTTACTCATCAGGTTCAACATTATTAGTTTTAGCACAAAAAGCTAAAGATGAAGTTGAAGCAAGAAAAATGATTGATGAAGTAATCAATAATAAAAAAGCATATAACAAATTTTTGGAATGA", "aa": "MSGRGLGHTGGTLDKLESIDGYNFSVTDQQFRDLVEKHGIAIVGQNQKLVPADKKIYALRDVTGTVQSIPLIASSIMSKKLATGSNCILLDVKCGNGAFMKDINEAKKLGKLMIEIGKKLNRKIAVEITNMQQPLGKTIGNKIEVLEAIDTLNGHGPKDFTEIIYSSGSTLLVLAQKAKDEVEARKMIDEVINNKKAYNKFLE", "aa_hexdigest": "c73401cc8d28be398ff1038470d4c93b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A386RHI4", "query_cov": 0.8275862068965517, "subject_cov": 0.8527918781725888, "identity": 0.53, "score": 182.0, "evalue": 3.36e-55, "valid": false, "product": "thymidine phosphorylase", "ec_ids": [ "2.4.2.4" ], "uniref50_id": "UniRef50_A0A386RHI4", "go_ids": [ "GO:0006206" ] }, "pscc": { "uniref50_id": "UniRef50_A0A386RHI4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A386RHI4" ], "product": "Pyrimidine-nucleoside phosphorylase" }, "genes": [], "id": "GLJLFKCOHC_742", "locus": "GCA013368035_00730" }, { "type": "cds", "sequence": "contig_1", "start": 416817, "stop": 417029, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02885.22" ], "nt": "ATGAGAATTATTGATATTATTAACAAAAAAGTAGAAAATCAAACTCTTACCAAAGAAGAAATTGATTTTTTCATTAAAGAATATGTAGCAGAAAATGTTCCAGACTACCAAGCTGCTGCTTTATTAATGGCAATCAGATTAAATGGTTTAAGCGATGCTGAAACAGCGCAATTAACCGATGCTATGATGCACTCAAGCGAAGTACTTGATTGA", "aa": "MRIIDIINKKVENQTLTKEEIDFFIKEYVAENVPDYQAAALLMAIRLNGLSDAETAQLTDAMMHSSEVLD", "aa_hexdigest": "179a365471df4faf18902bf43342eb38", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02885.22", "name": "Glycos_trans_3N", "length": 61, "aa_cov": 0.8714285714285714, "hmm_cov": 1.0, "evalue": 4.888982260166809e-25, "score": 82.54816436767578, "start": 5, "stop": 65 } ], "seq_stats": { "molecular_weight": 7929.981200000003, "isoelectric_point": 4.414707374572754 }, "id": "GLJLFKCOHC_743", "locus": "GCA013368035_00731" }, { "type": "cds", "sequence": "contig_1", "start": 417046, "stop": 417762, "strand": "-", "frame": 2, "gene": "deoD", "product": "purine-nucleoside phosphorylase", "db_xrefs": [ "COG:COG0813", "COG:F", "EC:2.4.2.1", "GO:0004731", "GO:0006139", "KEGG:K03784", "RefSeq:WP_104940477.1", "SO:0001217", "UniParc:UPI000CD6AA3C", "UniRef:UniRef100_UPI000CD6AA3C", "UniRef:UniRef50_A0A449A549", "UniRef:UniRef90_D1J8C3" ], "nt": "ATGACACCTCATATAAATGCAAAAGATGATGATTTTGCAAAATTAGTTTTAATGCCAGGTGATCCTTTAAGAGCAAAGTATATTGCTGAACACTATTTAGAAAACGCTAAGTTAGTGTCAACAGTTAGAAATGTTTTAATGTATACTGGCTTTTATAAAGGAAGAAAAATTTCTATTTGTGCTTCAGGCATGGGCGTTCCTTCAATTGGAATTTATTCATATGAATTATTTAGCCAATATGGAGTTGAAGCAATAATTAGAATTGGATCAGGTGGCTCATTCAAGGCTGATTTAAAAAATTATTCATTAGTTTTGGCAACTTCAGCATATAGTGATAGCACTTCATTCCGTAAAAATTTATTAGTCAATGATGAAAGTAATGTAGCTCTTCCAAATAGTGAATTAAATGATTCAATTAGAAAACATGCTAAGAAATTGAATTTAAAACTTGAAGAAGGAAGAATTTTATCGGAAGATGCCTTTTATTCAGTATTTTCTGTTGAAGATAGAGTTAAAAATTCAGGCGGAGCAGTTGCTGTTGAAATGGAAGCATATGGACTATTTACAGTAGCAGAAAAATTGCACAAAAAAGCTGCAACAATTTTAACTATTTCTGATAATTTAATAACACATGAATATACAACCGCTGAAGAAAGACAAAATTCATTCAATCAAATGATGGAATTAGCATTATCAATATCAGAAGATTTTGAATAA", "aa": "MTPHINAKDDDFAKLVLMPGDPLRAKYIAEHYLENAKLVSTVRNVLMYTGFYKGRKISICASGMGVPSIGIYSYELFSQYGVEAIIRIGSGGSFKADLKNYSLVLATSAYSDSTSFRKNLLVNDESNVALPNSELNDSIRKHAKKLNLKLEEGRILSEDAFYSVFSVEDRVKNSGGAVAVEMEAYGLFTVAEKLHKKAATILTISDNLITHEYTTAEERQNSFNQMMELALSISEDFE", "aa_hexdigest": "b43ede96731dc5bae5df14d599424b9f", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI000CD6AA3C", "ncbi_nrp_id": "WP_104940477.1", "uniref100_id": "UniRef100_UPI000CD6AA3C", "db_xrefs": [ "SO:0001217", "UniParc:UPI000CD6AA3C", "RefSeq:WP_104940477.1", "UniRef:UniRef100_UPI000CD6AA3C" ] }, "ips": { "uniref100_id": "UniRef100_UPI000CD6AA3C", "uniref90_id": "UniRef90_D1J8C3" }, "psc": { "uniref90_id": "UniRef90_D1J8C3", "gene": "deoD", "product": "purine-nucleoside phosphorylase", "ec_ids": [ "2.4.2.1" ], "uniref50_id": "UniRef50_A0A449A549", "cog_id": "COG0813", "cog_category": "F", "kegg_orthology_id": "K03784", "go_ids": [ "GO:0004731", "GO:0006139" ] }, "pscc": { "uniref50_id": "UniRef50_A0A449A549", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A549" ], "product": "Uridine phosphorylase" }, "genes": [ "deoD" ], "id": "GLJLFKCOHC_744", "locus": "GCA013368035_00732" }, { "type": "ncRNA", "class": [ "RNase_P_RNA", "SO:0000386" ], "sequence": "contig_1", "start": 417903, "stop": 418193, "strand": "-", "gene": "RNaseP_bact_b", "product": "Bacterial RNase P class B", "score": 207.6, "evalue": 2.6e-22, "db_xrefs": [ "RFAM:RF00011", "SO:0000386" ], "nt": "TTACATGGCCAACTGCTACTACAAAGTAGAGGAAAATCCGCTCTAGCACTCGCTGTGATGCGAGTAGTGTTCATGCTAGACCCAATAAGTCTAGGCAACAATTTAGTTGACGACTAGTGCAACAGAGACGAGCTTATTTAGTTAAGATGCAACGATGTAAACTCCATGAGCTAGAAACCCAAATTTTGGTAGGGGAACTGGGAACAAGGAATCGAACAAAGAACCCGGAAGCCGATTGGCTTAGATAAATAGTTGGCGCTATTAAAATAGTACAGAAAGCGGTTTATAGTG", "id": "GLJLFKCOHC_42", "locus": "GCA013368035_00733" }, { "type": "cds", "sequence": "contig_1", "start": 418260, "stop": 418814, "strand": "+", "frame": 3, "gene": "recO", "product": "DNA repair protein RecO", "db_xrefs": [ "COG:COG1381", "COG:L", "GO:0003677", "GO:0006281", "GO:0006310", "KEGG:K03584", "SO:0001217", "UniRef:UniRef50_D1J8C4", "UniRef:UniRef90_D1J8C4" ], "nt": "ATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATGTTAATGAATTTGATGAAATAATATCATTACTAACAAATAAAGGCATTCAAAGAATTTTTTGCCCCGGGGTAAATAAAACTAATTCAAAAAATGGCCCATCAATTTTAATTTTAAACTTAGTTAATTGTGAAGTCATTAAATCAGAAACAAGAAATTTATTACCTAGACTAAAAACTGCTTCGTTAATAAAAAATTTTCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAATTGTCTTGATAATTGCAATAATATTAATCTTATTAAAGAATATGATCATTGCTTAGATTTTATATTGTCAAAGTCTGATAAAGTTGAAGCGTATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCTAACTTAAATTCTTGTATTGAATGCAATAATGTTGAAAATATTGTAGATTTTAAACTATACAAAGGGGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCGCTATTAAGGTCTTGA", "aa": "MEFELSGIVINKQNVNEFDEIISLLTNKGIQRIFCPGVNKTNSKNGPSILILNLVNCEVIKSETRNLLPRLKTASLIKNFPTDYRLLNIANDLKTFFNCLDNCNNINLIKEYDHCLDFILSKSDKVEAYLLFLVVSSLGFKPNLNSCIECNNVENIVDFKLYKGGFLCSMHSNIVNADVSLLRS", "aa_hexdigest": "688f14d732a3bc9681cfcaa24671281c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8C4", "query_cov": 1.0, "subject_cov": 0.8214285714285714, "identity": 0.9890000000000001, "score": 365.0, "evalue": 3.22e-127, "valid": true, "gene": "recO", "product": "DNA repair protein RecO", "uniref50_id": "UniRef50_D1J8C4", "cog_id": "COG1381", "cog_category": "L", "kegg_orthology_id": "K03584", "go_ids": [ "GO:0003677", "GO:0006281", "GO:0006310" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C4" ], "product": "DNA replication/recombination mediator RecO N-terminal domain-containing protein" }, "genes": [ "recO" ], "id": "GLJLFKCOHC_745", "locus": "GCA013368035_00734" }, { "type": "cds", "sequence": "contig_1", "start": 418918, "stop": 419232, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "GTGGATAAGAATGAACATATTTTTAGTCAATATATTGAAGATAAACAAGACAAGGAAACATTGACTAAAATTTCTGATGATTTTAAAAATCTATATGAATATTTAGAAGATAATTTTAAACAATACTTACTATTTTATAATGAAGCATTTAATTTAATTATTAATTTACATAAAAAGAATATATCAAAGGAAGATTTAATAAAATTAAATGATTTTGAAGACATGTTATATTCTAATAAAACAAATAACATTGATTTTAAAACATTGCAACGTAAGTTTGATGAGATAAAAAAAATATCTATTAACGATAAATAA", "aa": "MDKNEHIFSQYIEDKQDKETLTKISDDFKNLYEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEDMLYSNKTNNIDFKTLQRKFDEIKKISINDK", "aa_hexdigest": "e672a6259f7bcbfe22b7665876df87f6", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 418918, "stop": 419532, "observations": { "mutation": [ 419292 ], "stop": [ 419292 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.4928909952606635, "identity": 0.9520000000000001, "score": 190.0, "evalue": 1.48e-59, "gene_start": 1, "gene_end": 104, "reference_start": 108, "reference_end": 211, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9705882352941176, "score": 394.0, "evalue": 1.18e-142, "description": "Internal stop codon at 419292. Nonsense mutation around 419292.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12743.227999999977, "isoelectric_point": 5.175156211853026 }, "id": "GLJLFKCOHC_746", "locus": "GCA013368035_00735" }, { "type": "cds", "sequence": "contig_1", "start": 419290, "stop": 419553, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5", "UniRef:UniRef90_D1J8C5" ], "nt": "ATGAAGTTATCAAAGAATCAAGAAAAGGTTCAAAACAAAAAACCACATAGATTTTTAACTAGAAGATCAAAAATAGGCATCATTTCAGGAATTAGTGCAGTTATTGGATTGCCTTTTTTGCTAGGTATTACTTATGTTCTTGTTAATAATCACAAAAAAACTAATTCAAACAGTCAATTAATAATAATTCGTAAAGAAATTTATAAGCAATATTTACGTTCATTAAATTTAATTCATTCATTGCCTAATTATGGTATATTTTGA", "aa": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIF", "aa_hexdigest": "b8fc13f867f179a3a78165c64892718f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 418987, "stop": 419553, "observations": { "mutation": [ 419292 ], "stop": [ 419292 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8C5", "query_cov": 1.0, "subject_cov": 0.41232227488151657, "identity": 1.0, "score": 171.0, "evalue": 2.88e-52, "gene_start": 1, "gene_end": 87, "reference_start": 1, "reference_end": 87, "reference_sequence": "MKLSKNQEKVQNKKPHRFLTRRSKIGIISGISAVIGLPFLLGITYVLVNNHKKTNSNSQLIIIRKEIYKQYLRSLNLIHSLPNYGIFRNLRDYFEKRVAQYDFKKKIVDKNEHIFSQYIEDKQDKETLAKISDDFKNLFEYLEDNFKQYLLFYNEAFNLIINLHKKNISKEDLIKLNDFEGMLRSNKTNNIDFKTLQRKFDEIKKISINDK" }, "paralog": false, "identity": 0.9680851063829787, "score": 362.0, "evalue": 2.65e-130, "description": "Internal stop codon at 419292. Nonsense mutation around 419292.", "psc": { "uniref90_id": "UniRef90_D1J8C5", "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8C5", "UniRef:UniRef50_D1J8C5" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8C5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8C5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 10047.819399999991, "isoelectric_point": 10.718271827697755 }, "id": "GLJLFKCOHC_747", "locus": "GCA013368035_00736" }, { "type": "cds", "sequence": "contig_1", "start": 419794, "stop": 420204, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17770.6" ], "nt": "ATGCCTTATCACGGTGAATATAGAATGAGCGTTGTTCATGGCTATACTGCTATTGAAAGCGGTGTTGATGCTAAAAACATTTTGATTGCTAGATTAGGGGATGTTTATTATTTAGAAAATCATCAAGTTCGTTTGTCGAACGAAAAGGTTTATTTCGGGCCTGTTTTTATTGATGGCAATATTTTATCAAAAACTAACTCTCAAATTATTAAAGAACGTAGTGAATTAGGCCAAAACGGATTTATGCATGTTGTTTTAGCAATTAATAAAGAAAAGAATCTAATTATTGGTAAACCAAGAATAGTTTCACGTGGCGCATTTTATGTAAAAAATTCATTGCATTTAATTGAAGAAGCTAAGAGATTGGTTCACGGCGCTATATTATATACAATTAAAAACTCAAATGATTGA", "aa": "MPYHGEYRMSVVHGYTAIESGVDAKNILIARLGDVYYLENHQVRLSNEKVYFGPVFIDGNILSKTNSQIIKERSELGQNGFMHVVLAINKEKNLIIGKPRIVSRGAFYVKNSLHLIEEAKRLVHGAILYTIKNSND", "aa_hexdigest": "20886ae1a00e5afdf394dd569c4a12bd", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17770.6", "name": "RNase_J_C", "length": 60, "aa_cov": 0.4411764705882353, "hmm_cov": 1.0, "evalue": 6.857367156081728e-14, "score": 48.14616012573242, "start": 69, "stop": 128 } ], "seq_stats": { "molecular_weight": 15400.56679999999, "isoelectric_point": 9.300031852722167 }, "id": "GLJLFKCOHC_748", "locus": "GCA013368035_00737" }, { "type": "cds", "sequence": "contig_1", "start": 420427, "stop": 420888, "strand": "-", "frame": 2, "gene": null, "product": "ribonuclease J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6", "UniRef:UniRef90_UPI003080D5B6" ], "nt": "ATGATGACAGGCGATTTTAGGTTTGACTATACACCTATTGGAAATTTTACAGATTTTAATAAACTTAAACAAATTGGCGATGAAGGTCTTGATGTTTTATTTTCTGATTCAACAAATGCTATGCGTCCAAATCATTCACCATCAGAGTCAGATATTTTAAAAGATATTAAAAAATATATGTTGGAAGCAGAAAAGAAAATCATTGTAACTGCATTTGCTTCTAATTTAACTAGAATTAAAGCAATTATTGAATTAGGCGCTTCTTTAAATAAAAAGGTTGTTGCCTTCGGCCGTTCTATGGTTGATGGAATAGATATTGGTAGAAGACTAGGTTATATTAATGTTCCAGATGAAGTGTTTATTGACAAAAAATCATTAAGCAAATTTGGAGATAATGAGTTATTAATTTTAACAACTGGTTCTCAAGGTGAAGAACTAGCTGGACTTGCAAAGATGTCATGA", "aa": "MMTGDFRFDYTPIGNFTDFNKLKQIGDEGLDVLFSDSTNAMRPNHSPSESDILKDIKKYMLEAEKKIIVTAFASNLTRIKAIIELGASLNKKVVAFGRSMVDGIDIGRRLGYINVPDEVFIDKKSLSKFGDNELLILTTGSQGEELAGLAKMS", "aa_hexdigest": "e42de43bff8d4d1683c236ec3a4bfa47", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 420472, "stop": 421020, "observations": { "mutation": [ 420954, 420984 ], "stop": [ 420954, 420984 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003080D5B6", "query_cov": 0.9019607843137255, "subject_cov": 0.7540983606557377, "identity": 0.855, "score": 233.0, "evalue": 2.82e-76, "gene_start": 1, "gene_end": 138, "reference_start": 46, "reference_end": 183, "reference_sequence": "TNVEFIEINKDLVVKFGECSDDWWTAQHSIPDAFGVRVKTPTGSLMLTGDIRFDYTPIGNLTDLSKLMKIGEEGLDVLFSDSTNAMRPNHSPSASDILKDIKKYMLEAEKKSIVTAFASKLTRIKAIIELGASLGKKVIAFGRSMIYNIDIGRRLGYINAPDEVFIDKKHLSKYNDNELLILT" }, "paralog": false, "identity": 0.8131868131868132, "score": 295.0, "evalue": 4.34e-103, "description": "Internal stop codon at 420954, 420984. Nonsense mutation around 420954, 420984.", "psc": { "uniref90_id": "UniRef90_UPI003080D5B6", "product": "ribonuclease J", "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003080D5B6", "UniRef:UniRef50_UPI003080D5B6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003080D5B6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003080D5B6" ], "product": "ribonuclease J" } }, "id": "GLJLFKCOHC_749", "locus": "GCA013368035_00738" }, { "type": "cds", "sequence": "contig_1", "start": 420982, "stop": 421353, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00753.33" ], "nt": "ATGAGCCCAACTAAAATTTTTGGATTAGGCGGAATGCAAGAAATTGGTAAAAGTACTTTGGTTATTCAATATGAAAATCATATTTTTATTATTGACACCGGAATTAAATTTTGCGATTCATATGTAACAGGTGTAAATGGTTCTATTCCAGATTTCACTTTTTTAGCAGAAAACCAAGATAAAATCGAAGGTCTTTTCATAACCCACGGACATGAAGATCATATTGGAGGTGTTCCATATTTAGTTCAACAAGTAGATGTTAAAAAAATATTTGCACCTAGAATTGCTATTCAATATCTAAAACTAAAATTTGAGGATATGAAGATAAAACGTAATATTGAATTTATTGAAACTGAAAAAGATGCAGTTTGA", "aa": "MSPTKIFGLGGMQEIGKSTLVIQYENHIFIIDTGIKFCDSYVTGVNGSIPDFTFLAENQDKIEGLFITHGHEDHIGGVPYLVQQVDVKKIFAPRIAIQYLKLKFEDMKIKRNIEFIETEKDAV", "aa_hexdigest": "5657dcf5c352ec4d827c4c65c5ef9a20", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00753.33", "name": "Lactamase_B", "length": 77, "aa_cov": 0.6260162601626016, "hmm_cov": 0.9090909090909091, "evalue": 5.2638570814702125e-12, "score": 41.480987548828125, "start": 18, "stop": 94 } ], "seq_stats": { "molecular_weight": 13935.941499999986, "isoelectric_point": 5.498797416687012 }, "id": "GLJLFKCOHC_750", "locus": "GCA013368035_00739" }, { "type": "cds", "sequence": "contig_1", "start": 421472, "stop": 421594, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGATAGTAGGGTACAAAAAACAAAGATTCCTAAAGTATTAGCAAGTTTTAGTTTAATTTTAGAACTTATTGCATTAGCATTACTTATTTATGTTATATATAGTTTCTCTTCAATTTGA", "aa": "MNDSRVQKTKIPKVLASFSLILELIALALLIYVIYSFSSI", "aa_hexdigest": "13087fb91022485fd00f91ad0075f999", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4499.399499999999, "isoelectric_point": 9.398732948303223 }, "id": "GLJLFKCOHC_751", "locus": "GCA013368035_00740" }, { "type": "cds", "sequence": "contig_1", "start": 421690, "stop": 421917, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAATTTTTTTGCTTTTTGATTATTATTCAAAATAGTGATATTATTCTAACTATTTCACATAATAATAGAATTGGCAAACATAATGTAACAATAATAAGTATTATTGGAACTTTGTGTAATGACCAACTAGCTTTTGCTATTACTAACATGCTAAATATAAAGCAAACATATAATAATATTGGCAATGCTAAAATTGCACTAATTAAAACTTTATTATTTTTATAA", "aa": "MKFFCFLIIIQNSDIILTISHNNRIGKHNVTIISIIGTLCNDQLAFAITNMLNIKQTYNNIGNAKIALIKTLLFL", "aa_hexdigest": "cf4786a95e9ed728adcc40b4a0be2989", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8464.041299999992, "isoelectric_point": 9.51168155670166 }, "id": "GLJLFKCOHC_752", "locus": "GCA013368035_00741" }, { "type": "cds", "sequence": "contig_1", "start": 422029, "stop": 422964, "strand": "-", "frame": 2, "gene": "trxB", "product": "Thioredoxin reductase", "db_xrefs": [ "COG:COG0492", "COG:O", "RefSeq:WP_020002482.1", "SO:0001217", "UniParc:UPI00037C1753", "UniRef:UniRef100_A0A6A8PY71", "UniRef:UniRef50_A0A448ZW45", "UniRef:UniRef90_D1J8C8" ], "nt": "ATGGAAAATAATAACTTATATGATGTTTTAATAATTGGGGCAGGGCCGGCAGGATTAACAGCTGCAATATATTTAGCAAGAAATGACATAAATGTTGCTTTTATTGAAGGCGGAGTACCGGGAGGAAAAGTTTCTGCCCAATCTAAAATTGAAAATTATCCAGGTTTTAGTTTTATAAGTGGTTTTGAACTTTCTAAAGCTATGCTTAAACAAGCAAGAGATAATGGCTCAAAATTTATTTACGGTAATGTAAAGGAAATTAATTCTATATCAGATATTGAAAAAGAAGTCATTTTGGAAAATGGCATGTCAATAAAATCAAAAGCAATAATTATTGCAACTGGCATGAAAGAAATTGTTCCTTTGGATGTAAAAGGTATTGAAGAATATAGAGGCAAAGGTGTATCATATTGCGTAATTTGCGACGGTCCTTTATATAAAAATAAACCCTGTGCAATCATTGGTGGAGGTAATTCAGCATTCGAAGAATCGTCTTATTTAGCATCAATTGCCTCTGAGGTTCATATTTTTGTTCGTGATGGAATTATTGCAGAAAAAAAACTTGTCAATGAAGCAATGTCTAAAAGCAATATTTATATTCATGAAAATTCAACTATTAAAGAAATTTTGGGCAATGAAAAAGTTGAAGAAATTATTGCTAATGTAAATGGAAAAGAAACAAAAATGAATATAAGTTGCATTTTTCCTTATGTTGGTTTTAAACCTAACACAGCGTTTATAAAAGGCAAATACGATGAAATATTAAATAGCCGTGGATTTATTGAAGTTAATGAAAATATGGAAACTAAAATTCCTAATATTTTTGCAATAGGCGATGTTATTGTAAAAGATGTAAGACAAATTACAACAGCAACAGCTGATGGGACAATTGCTGCAAGAGCAATAAGTAATAAAATCGATAAATTATATAAATAA", "aa": "MENNNLYDVLIIGAGPAGLTAAIYLARNDINVAFIEGGVPGGKVSAQSKIENYPGFSFISGFELSKAMLKQARDNGSKFIYGNVKEINSISDIEKEVILENGMSIKSKAIIIATGMKEIVPLDVKGIEEYRGKGVSYCVICDGPLYKNKPCAIIGGGNSAFEESSYLASIASEVHIFVRDGIIAEKKLVNEAMSKSNIYIHENSTIKEILGNEKVEEIIANVNGKETKMNISCIFPYVGFKPNTAFIKGKYDEILNSRGFIEVNENMETKIPNIFAIGDVIVKDVRQITTATADGTIAARAISNKIDKLYK", "aa_hexdigest": "363540d965586c386bd51304437db1bf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI00037C1753", "ncbi_nrp_id": "WP_020002482.1", "uniref100_id": "UniRef100_A0A6A8PY71", "db_xrefs": [ "SO:0001217", "UniParc:UPI00037C1753", "RefSeq:WP_020002482.1", "UniRef:UniRef100_A0A6A8PY71" ] }, "ips": { "uniref100_id": "UniRef100_A0A6A8PY71", "uniref90_id": "UniRef90_D1J8C8" }, "psc": { "uniref90_id": "UniRef90_D1J8C8", "gene": "TrxB", "product": "Thioredoxin reductase", "uniref50_id": "UniRef50_A0A448ZW45", "cog_id": "COG0492", "cog_category": "O" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZW45", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZW45" ], "product": "Thioredoxin reductase" }, "genes": [ "trxB" ], "id": "GLJLFKCOHC_753", "locus": "GCA013368035_00742" }, { "type": "cds", "sequence": "contig_1", "start": 422998, "stop": 423255, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAACCTTTACGTGATGGCAGTTATACTATTTATAAAGTAATTTCTGCTTTATATATAATAGTTGGTTTGGCATTGACTGTAATGTTTGAATTCGTTATAAAATTGAATTACAATGTTTACAAAGTTAATACATATAATAACGAAAAGCTTTCAAAATTCTTTTACTTTATAGTTTATGAACCTAAAAATTCAAATTTATTTACATTTAAAAAATCGACTACTAAAAATCAAATAGAAACTAAAACAGCAGCTTAA", "aa": "MEPLRDGSYTIYKVISALYIIVGLALTVMFEFVIKLNYNVYKVNTYNNEKLSKFFYFIVYEPKNSNLFTFKKSTTKNQIETKTAA", "aa_hexdigest": "54112530507a54eaae7ac8d67a19d4be", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9970.511299999987, "isoelectric_point": 9.456045341491702 }, "id": "GLJLFKCOHC_754", "locus": "GCA013368035_00743" }, { "type": "cds", "sequence": "contig_1", "start": 423337, "stop": 423474, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAAGGTTCTGTCGCATTTGAAATATTGCCAAAATTAATTAGAGATCATATGTTTATTGATGGAAAATATCGTTATCCTTTATTTTTCTATGAATCAATTGCAAACTTAGCTGCTTATATTATCTTAGTTTGA", "aa": "MEKGSVAFEILPKLIRDHMFIDGKYRYPLFFYESIANLAAYIILV", "aa_hexdigest": "73895c3ec48eb0fb9b843951e168c0dc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5297.237099999999, "isoelectric_point": 6.526503181457519 }, "id": "GLJLFKCOHC_755", "locus": "GCA013368035_00744" }, { "type": "cds", "sequence": "contig_1", "start": 424018, "stop": 426063, "strand": "-", "frame": 2, "gene": null, "product": "UvrABC system protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "nt": "ATGCCAAAAATTGAACCCAAATTATTCTCGTTTAATTCTCCAGCCGGAATGTGTCCTGAATGCAAGGGACTTGGTGTTATTCAAAAAGCAAGCTTTGATTTAATTTGTCCCGATAGGCAGCTTTCTATAAACGAAGGAGCAATAAAGTATTATAGAAATTTCATGAATACTTACAATTTAGAATGGCAAGAATTTGACAAGCTATTAAATTATTATCATATTGATAAAGACCAACCCATTGATTCTCTATCTTCTTTTGAATTAGATATTATTCAACATGGGTCGCAAGAAGAAATATCATATGTGTTATTATCTGAAAGTGGTACAAAACGCGAGAAGTTTCAAGTTATTGAAGGTATTGTTGATAAATTACAAAGGAAGTATTTTGATACAAAATCACTAGAAGCTAGGGCTTATTATAGTAAATACATTGGAGATATTACTTGTCCTTCATGCCATGGAAAGCGTTTAAATAAGTATGCGCTGTCTTGCAAAATTCTAGATCTAGATATATATGAAATTTGCAATAAGTCGCTTGGGGATTTAGCAATTATTTTAGAAAGATTGCAGTTAAGTGATTTAGAAAAAGAAATTACCTCTTTAATTTTGTCTGAATTAAAACATAGAATTAAATTTTTAATTGATGTTGGCTTAAAGTATTTAACATTGAATAGAAAAGCTGAGACATTAAGCGGTGGTGAAGCTCAAAGAATTAGATTAGCAACACAAATTGGCTCGAATCTAACAGGCATTTTATATGTTTTAGATGAACCATCAATTGGCTTACATCAAAAAGATAATGAAAAATTGCTAAACTCTTTAAGAAAAATGGTTGAATTAGGTAATAGTTTAATTGTTGTTGAACATGATGAAGAAACAATTAGACAAGCAGATTATATAGTTGATATTGGTGTTCAAGCAGGCGAACATGGTGGAAATATTGTTGCGTGTGGAAATGTTGAAAATGTAATTGCATGTAAGCAAAGTCTAACTGGCCAATATTTGTCAAAAGAAAGACAAATAGAGGTGCCAAAATTTAGGCGGAGTGGAAATGGCAAGACATTAACATTAAAAAATGCAAAATTAAATAATTTAAAAAATATTAAAGTAACTTTTCCGTTAGGAAAATTTATTGCAATTACTGGAGTTAGTGGTTCTGGAAAATCTACATTAATAAACGATGAACTAGTTGGAAGTCTTAATAAATATTTATTAGATCAATATTATGATATTCAAAGAGATAATAAACTAATTGGCCAAACTAACATTGATAAATTAATTCAAATTGACCAAAATCCAATCGGGCGTACTCCTAGATCAAATCCTGCAACATATACCGGTGTTTTTGACGATATTAGAGAAATTTTTGCAAATACCGAAGAAAGTAAAATTAGAGGATATACAAAATCAAGATTTTCATTTAATGTTCCAGGTGGGCGTTGTGATAAATGCGAAGGCGATGGCGTAATCAAAATTGCTATGCATTTTTTGCCCGATGTTTATGTGACATGCGACCATTGCAACGGGAAACGTTATAATCAAGAAACGCTAGAAATAAAATATCATGGTAAAAGTATTGGCGATGTTTTGGATATGACAATTGAAAAAGCCTATGCATTATTTATAAACAAGAGCCAAATTAAAGAAAAATTGCAAGTTTTATTAGATGTTGGACTTGGCTATATTAAATTAGGCCAAAATGCTACGACATTAAGCGGTGGTGAAGCTCAAAGGGTAAAATTAGCAACATACCTACAAAAAAAGCCAACAGGCAAATCTTTATATGTATTAGATGAACCAACAACTGGATTACATCCTTACGATATCTCTAATTTATTAATAGTATTAAATAGAATAGTGGACAATGGCGATACAGTAATTGTAATTGAACATAATTTGGATTTAATTAAATGTGCTGATCATATTATTGATTTAGGCCCTGATGGTGGGGACGAAGGAGGAAAAGTTATTTGCTCAGGAACACCTGAACAGGTCTCAAAATGTGAAAATTCGTACACGGGGCAATATTTAGTAAAACTTTTATAG", "aa": "MPKIEPKLFSFNSPAGMCPECKGLGVIQKASFDLICPDRQLSINEGAIKYYRNFMNTYNLEWQEFDKLLNYYHIDKDQPIDSLSSFELDIIQHGSQEEISYVLLSESGTKREKFQVIEGIVDKLQRKYFDTKSLEARAYYSKYIGDITCPSCHGKRLNKYALSCKILDLDIYEICNKSLGDLAIILERLQLSDLEKEITSLILSELKHRIKFLIDVGLKYLTLNRKAETLSGGEAQRIRLATQIGSNLTGILYVLDEPSIGLHQKDNEKLLNSLRKMVELGNSLIVVEHDEETIRQADYIVDIGVQAGEHGGNIVACGNVENVIACKQSLTGQYLSKERQIEVPKFRRSGNGKTLTLKNAKLNNLKNIKVTFPLGKFIAITGVSGSGKSTLINDELVGSLNKYLLDQYYDIQRDNKLIGQTNIDKLIQIDQNPIGRTPRSNPATYTGVFDDIREIFANTEESKIRGYTKSRFSFNVPGGRCDKCEGDGVIKIAMHFLPDVYVTCDHCNGKRYNQETLEIKYHGKSIGDVLDMTIEKAYALFINKSQIKEKLQVLLDVGLGYIKLGQNATTLSGGEAQRVKLATYLQKKPTGKSLYVLDEPTTGLHPYDISNLLIVLNRIVDNGDTVIVIEHNLDLIKCADHIIDLGPDGGDEGGKVICSGTPEQVSKCENSYTGQYLVKLL", "aa_hexdigest": "20105736033ab33d75da10189a055714", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P0N4", "query_cov": 0.8854625550660793, "subject_cov": 0.9934102141680395, "identity": 0.735, "score": 891.0, "evalue": 0.0, "valid": false, "gene": "uvrA", "product": "excinuclease ABC subunit UvrA", "uniref50_id": "UniRef50_A0A3B0P0N4", "kegg_orthology_id": "K03701", "go_ids": [ "GO:0003677", "GO:0006289", "GO:0009380", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P0N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P0N4" ], "product": "UvrABC system protein A" }, "genes": [], "id": "GLJLFKCOHC_756", "locus": "GCA013368035_00745" }, { "type": "cds", "sequence": "contig_1", "start": 426113, "stop": 426295, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTTCATTAACAATAGTTGAAATTAAACCATTAGATTGCTCTAATGCAACCTCAATTGCACTTGATATTCTTGATTTTATATCATCATCATTTGATAATTTAACTCTATCAATAAATAGATCAATATTTCAACGTTTATTTTTATCTAGATTAATTGAATCAACATTTTCTAAGAAGTAA", "aa": "MLSLTIVEIKPLDCSNATSIALDILDFISSSFDNLTLSINRSIFQRLFLSRLIESTFSKK", "aa_hexdigest": "cd9470a13223746c5e35c9ad660c34c2", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6767.816499999995, "isoelectric_point": 6.024842262268066 }, "id": "GLJLFKCOHC_757", "locus": "GCA013368035_00746" }, { "type": "cds", "sequence": "contig_1", "start": 426244, "stop": 426846, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF17760.6" ], "nt": "ATGAACGATAAAATAATAATAAAAGGTGCTAGAGAAAATAATTTAAAAAATATTAATTTAGATATTCCACGTAACAAATTTGTGGTTTTTACTGGTGTCAGTGGCTCTGGTAAAAGTTCTTTAGCATTTAATACAATCTATGAAGAAGGCAGACGTAGGTATGTTGATAGCCTTTCTAATTATGCAAAACAATTTTTAGGTGGTACTAAAAAACCTAATGTTGATAGCATTGAAGGATTGAGCCCAGCAATTTCTATTGAACAAAAAACAGTACATGTTAATCCCAGAAGTATAGTTGGTACTGTTACTGAAATTTATGATTATTTAAGATTGCTATATGCAAGAGTAGCAAAACCTTATTGTCCAAATCACAAAATTGAAATTACTGCTCAAAAAATCAAAGACATTATTTCTAACATTTATAAAAACCCATTAGGATCAACAATATACATATTAGCCCCATTAGTAAATAATCAAAAAGGAAGCCACCAAGTATTACTTAATAAATTAAAAAGAGATGGTTTTATAAAAGTTTTAATAAATGATGAGATTTACTTCTTAGAAAATGTTGATTCAATTAATCTAGATAAAAATAAACGTTGA", "aa": "MNDKIIIKGARENNLKNINLDIPRNKFVVFTGVSGSGKSSLAFNTIYEEGRRRYVDSLSNYAKQFLGGTKKPNVDSIEGLSPAISIEQKTVHVNPRSIVGTVTEIYDYLRLLYARVAKPYCPNHKIEITAQKIKDIISNIYKNPLGSTIYILAPLVNNQKGSHQVLLNKLKRDGFIKVLINDEIYFLENVDSINLDKNKR", "aa_hexdigest": "0885d6ec4577bc456b8074a8adf2108e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_W7DIQ3", "query_cov": 0.99, "subject_cov": 0.9611650485436893, "identity": 0.5710000000000001, "score": 233.0, "evalue": 5.36e-75, "valid": false, "product": "UvrABC system protein A", "uniref50_id": "UniRef50_W7DIQ3" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF17760.6", "name": "UvrA_inter", "length": 69, "aa_cov": 0.345, "hmm_cov": 0.9710144927536232, "evalue": 1.1868786515069206e-19, "score": 65.65689849853516, "start": 131, "stop": 199 } ], "seq_stats": { "molecular_weight": 22624.817300000024, "isoelectric_point": 9.641972160339353 }, "id": "GLJLFKCOHC_758", "locus": "GCA013368035_00747" }, { "type": "cds", "sequence": "contig_1", "start": 426992, "stop": 427549, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DDAE5", "UniRef:UniRef90_UPI001E2DDAE5", "PFAM:PF07804.18" ], "nt": "GTGGATGGCGAAATTCATTTATATAAACGAAACTCAAAATTTGCAAATAAAAACTTATACGATGTTTATAGTGAATATTTTGCAAGTCAAGTTGCCAAATTTTTGAATTTATCGGCGGTTGATTATAAAATTACTAAGTATGGAAAGCATTTTGTTAGTGATTGCAAATTGTTTACAAACAAAGATTTTTCATTTATCCCATTTTCTAAAATCATAAATCCCAATAGTATTGATAATAATTTAAAACTATGTAGTCAGATTGCTTTTTATTATGGCAAAGAGTTATTTGAAGATTTAATGGTTTTTGATGCTTTAATTTGCAATGGGGATAGACATTTAAATAATTTTGGCATGATGTTGGATAATAATAGTGGAATTTTAACAAAGCCAGCTCCAATATTTGATTTCAACAATAGTTTAATATTTGATTATCCTATTTATTTAAAATCAATTGCAAAAACTGCATTTTTAAATGACTATGAGAATCGCAAGACTAGGTTTTTTGAAAGTTTTAATCAACAACTAAAAATTTTTATTAGAAAAAGACATTTAGATTGA", "aa": "MDGEIHLYKRNSKFANKNLYDVYSEYFASQVAKFLNLSAVDYKITKYGKHFVSDCKLFTNKDFSFIPFSKIINPNSIDNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLD", "aa_hexdigest": "2a894863fe4c6149e219612a5b5518c3", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 426836, "stop": 427750, "observations": { "mutation": [ 426994, 427558, 427588, 427699 ], "stop": [ 426994, 427558, 427588, 427699 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E2DDAE5", "query_cov": 1.0, "subject_cov": 0.6085526315789473, "identity": 0.9890000000000001, "score": 375.0, "evalue": 8.64e-130, "gene_start": 1, "gene_end": 185, "reference_start": 68, "reference_end": 252, "reference_sequence": "MCNSFWICSQEESCKLNWEDVNYFDHFNNDSCNYFLDYKAKLTETNRLQIKSPSWFIDGDMLKAWVEVDGEIHLYKRNSKFANKNLYDVYSEYFASQVAKFLNLSAVDYKITKYGKHFVSDCKLFTNKDFSFIPFSKIIDPNSIDNNLKLCSQIAFYYGKELFEDLMVFDALICNGDRHLNNFGMMLDNNSGILTKPAPIFDFNNSLIFDYPIYLKSIAKTAFLNDYENRKTRFFESFNQQLKIFIRKRHLDWIKKLKKFKFKQPNKIKMDMKYLKFINKFFKKRIKKAEQYLIEKGYIKENER" }, "paralog": false, "identity": 0.9736842105263158, "score": 607.0, "evalue": 7.04e-224, "description": "Internal stop codon at 426994, 427558, 427588, 427699. Nonsense mutation around 426994, 427558, 427588, 427699.", "psc": { "uniref90_id": "UniRef90_UPI001E2DDAE5", "uniref50_id": "UniRef50_UPI001E2DDAE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E2DDAE5", "UniRef:UniRef50_UPI001E2DDAE5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E2DDAE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E2DDAE5" ] } }, "hypothetical": true, "pfams": [ { "id": "PF07804.18", "name": "HipA_C", "length": 103, "aa_cov": 0.5405405405405406, "hmm_cov": 0.9029126213592233, "evalue": 1.313309043026944e-09, "score": 33.39656448364258, "start": 77, "stop": 176 } ], "seq_stats": { "molecular_weight": 21784.72090000001, "isoelectric_point": 8.824706459045409 }, "id": "GLJLFKCOHC_759", "locus": "GCA013368035_00748" }, { "type": "cds", "sequence": "contig_1", "start": 427865, "stop": 428011, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAACATATTATTTAAAATGTAAAAACAAGATTTGTTTAAGTTTTGAAGCAGATGAAAAATTAACAACATTGAGCAATGTTAAATTGCTTGATGATTTTATTTTTATAAAGCAAAAATATAATGAATATTCACCAAGACAATGA", "aa": "MKTYYLKCKNKICLSFEADEKLTTLSNVKLLDDFIFIKQKYNEYSPRQ", "aa_hexdigest": "366f812ebc9389176d9af3cf518abcec", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5812.731900000001, "isoelectric_point": 8.97594928741455 }, "id": "GLJLFKCOHC_760", "locus": "GCA013368035_00749" }, { "type": "cds", "sequence": "contig_1", "start": 428018, "stop": 428176, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BCA40", "UniRef:UniRef100_Q8RK97", "UniRef:UniRef50_A0A454CA89", "UniRef:UniRef90_A0A454CA89" ], "nt": "ATGCTAGAAAATTTACAAAAAATATTGCATCGTGATCTTTCAAAAGAAATTGCAGAAGTTGAAGAAAAGTATAAAAATCAAGCAAAGGAAGACCACAAAGAAGATCCAAAAACAAAATCAAAAGATAATAAGAACGATTTTGATGGCGAAATAAATTAG", "aa": "MLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN", "aa_hexdigest": "9bddfb4c4a295b23be16d0370265a4b8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00000BCA40", "uniref100_id": "UniRef100_Q8RK97", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000BCA40", "UniRef:UniRef100_Q8RK97" ] }, "ips": { "uniref100_id": "UniRef100_Q8RK97", "uniref90_id": "UniRef90_A0A454CA89" }, "psc": { "uniref90_id": "UniRef90_A0A454CA89", "uniref50_id": "UniRef50_A0A454CA89" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA89", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA89" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6184.768400000002, "isoelectric_point": 5.288891029357909 }, "id": "GLJLFKCOHC_761", "locus": "GCA013368035_00750" }, { "type": "cds", "sequence": "contig_1", "start": 428186, "stop": 428608, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE", "UniRef:UniRef90_UPI001F2C38EE" ], "nt": "ATGTCAATAGAAGATTATGATGGTGGTTTTGGAACTAAGGAATTTTCTCTTGAATCTGAATTATCAGAAGACGAAGAATCTGATGAAAAATCAGATGATGGCGATTCAAATGATGAAGAAATAGATATGAGCAGTATGGATGATTTTAAAAATTCATTTGTAAATTTCTTAGAAGGTTTTGATGATTTAAAGCAACAAGCAAAAAAATTAAAAGAACAAATTGCTGATAATAAATTTATGTCTGGTTCTCCATTTGAAATGAATTATGAAGATCTAAGTATGGAAAAATTGGTTGATAGTATGTATAAAGCTGCCAAAGCAATTGGAAATGTTCGTGATGACGAAATGGTTGATTATTTCAAGGGCGCATTCGATGAACTAAGGGGAATGGGAATAAATAATATTCCTAATGATGGTTCATGA", "aa": "MSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNDEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGS", "aa_hexdigest": "ff5801b1df743f872f7cfd637333fe06", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 428018, "stop": 428681, "observations": { "insertion": [ 428624 ], "stop": [ 428188 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2C38EE", "query_cov": 1.0, "subject_cov": 0.6363636363636364, "identity": 0.993, "score": 263.0, "evalue": 1.09e-87, "gene_start": 1, "gene_end": 140, "reference_start": 25, "reference_end": 164, "reference_sequence": "MKFSREFIGKQNLSNRRSIPIERRMSIEDYDGGFGTKEFSLESELSEDEESDEKSDDGDSNEEEIDMSSMDDFKNSFVNFLEGFDDLKQQAKKLKEQIADNKFMSGSPFEMNYEDLSMEKLVDSMYKAAKAIGNVRDDEMVDYFKGAFDELRGMGINNIPNDGSWEKSMLENLQKILHRDLSKEIAEVEEKYKNQAKEDHKEDPKTKSKDNKNDFDGEIN" }, "paralog": false, "identity": 0.9772727272727273, "score": 400.0, "evalue": 4.72e-144, "description": "Internal stop codon at 428188. Frameshift due to insertion around 428624.", "psc": { "uniref90_id": "UniRef90_UPI001F2C38EE", "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2C38EE", "UniRef:UniRef50_UPI001F2C38EE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2C38EE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2C38EE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15832.989399999991, "isoelectric_point": 4.0500284194946286 }, "id": "GLJLFKCOHC_762", "locus": "GCA013368035_00751" }, { "type": "cds", "sequence": "contig_1", "start": 428780, "stop": 429151, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAGATTTAAACACTTTTAATTCCTTAAATATTTTTAATGATTTTTTAATAAACGACGATGAAGAAAATAAAAATTCTTTTGACTTTAAAAAATATGGACTTGAACAAAATGAAATTATTTTCTCTGCAATTTTACCTACAAAAGAAATTATTTCATTAGCATTAAAAATTGATTTTGTTTATGATGATAAAAGAGAATTTGATGAAGATTTTCAAATTGATCCTGAATTAATTAGTCAAAGTGTAATTTCTGAAAGAAAAGAAACTTTAAAATATTTAAAAGAATTAAAGCAAAATGAATTTAAAAAATTTCACACAAACCCTTCAAATGATATTAAAAATATAACTAGAAGTGATTTTAGCAAATGA", "aa": "MSDLNTFNSLNIFNDFLINDDEENKNSFDFKKYGLEQNEIIFSAILPTKEIISLALKIDFVYDDKREFDEDFQIDPELISQSVISERKETLKYLKELKQNEFKKFHTNPSNDIKNITRSDFSK", "aa_hexdigest": "2327adaa295d0b22f2aed847103bcbb6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14600.144199999979, "isoelectric_point": 4.6740636825561515 }, "id": "GLJLFKCOHC_763", "locus": "GCA013368035_00752" }, { "type": "cds", "sequence": "contig_1", "start": 429230, "stop": 429439, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAAGAAACAATTAGGAATAAATCTAAAATTAACAGTTAGAGAATTAAATGTAGCTTTTGACTACGAATTTAAACCAATAGAAGCAGTTAAAAAAATTAATTTTGAACCAATATCATTTCCAATAACTTCGGGGATTTCTTTTTCTGAATTAGTGGATGTTTTAGGCGCATTTTTTGGTTTTGAAAAGAGCGTGGTATTTCATTGA", "aa": "MIKKQLGINLKLTVRELNVAFDYEFKPIEAVKKINFEPISFPITSGISFSELVDVLGAFFGFEKSVVFH", "aa_hexdigest": "8369d173a9642d4d732bab994d7713c5", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7838.120199999998, "isoelectric_point": 6.552023887634278 }, "id": "GLJLFKCOHC_764", "locus": "GCA013368035_00753" }, { "type": "cds", "sequence": "contig_1", "start": 429432, "stop": 429713, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGTTGGAAAATGTTTACAAGAATGTAGAAACATCTAAAGATGAAATAATGACAATCGGGGATGGAAATAATGATATACCAATGTTGTCAATATCAAAATATTCTTATGCAATGGCAAATGCAACACAAACTGTACATGGACATAGCCACTTTCATACAAGTGCTGTTGAACAAAATGGATTGGGAGAAGCAATACTTGATTACATATATAGATTAAAAAATATAACCAAAAAATATATGTTGCATGAATTTGAAAACAATAGAGGAGCAGAAAATGATTAA", "aa": "MLENVYKNVETSKDEIMTIGDGNNDIPMLSISKYSYAMANATQTVHGHSHFHTSAVEQNGLGEAILDYIYRLKNITKKYMLHEFENNRGAEND", "aa_hexdigest": "363f7ce5717237b86bd4564abeeb1e3a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 64, "aa_cov": 0.6881720430107527, "hmm_cov": 1.0, "evalue": 7.2928345091288e-18, "score": 60.576683044433594, "start": 2, "stop": 65 } ], "seq_stats": { "molecular_weight": 10582.667599999993, "isoelectric_point": 5.4181428909301745 }, "id": "GLJLFKCOHC_765", "locus": "GCA013368035_00754" }, { "type": "cds", "sequence": "contig_1", "start": 430077, "stop": 430352, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08282.18" ], "nt": "ATGAGTCAAACAATTTTAAAAGTTACTAACAACAAGAAGCCAATTGCTTTTTCAGATGTTGACGGTACTATATATAAAAATTTTAATTTAAAGCAAGAAACTATTGATGATATCCATTTTGCAGTAGACAATGGATTAGATTTTAATATATGCACCGGAAATCCAATTCAAGAAAGAATGTTAAAACTAGCCGAAACATTAAAAGCAAGATATTTAATAGGATCTTCCGGCGCTCAAATTTTTGATATGCAATCAAAAAAATATATTGCCTGTTGA", "aa": "MSQTILKVTNNKKPIAFSDVDGTIYKNFNLKQETIDDIHFAVDNGLDFNICTGNPIQERMLKLAETLKARYLIGSSGAQIFDMQSKKYIAC", "aa_hexdigest": "86710d71722aa41631f71aaad54af27a", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08282.18", "name": "Hydrolase_3", "length": 75, "aa_cov": 0.8021978021978022, "hmm_cov": 0.9733333333333334, "evalue": 1.1064310812542464e-10, "score": 37.04678726196289, "start": 17, "stop": 89 } ], "seq_stats": { "molecular_weight": 10247.693499999992, "isoelectric_point": 7.745813179016113 }, "id": "GLJLFKCOHC_766", "locus": "GCA013368035_00755" }, { "type": "cds", "sequence": "contig_1", "start": 430369, "stop": 431172, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_112636580.1", "SO:0001217", "UniParc:UPI000DCB2741", "UniRef:UniRef100_UPI000DCB2741", "UniRef:UniRef50_A0A454CA04", "UniRef:UniRef90_A0A454CA04" ], "nt": "ATGAATAAAAAACAAATAAAAAAATTTTTAAGTTTTTCATTGGCTATTCCGACTTTACTAACTATCCCATTAGTTTCATTATCATGCAAAAAATCAAAAGATAATCAAGATAATTTAGAATTAGGCAATGATGACGGAATTGAAAAACAAGATTTAAGTGTTGCCAAAAGAATAGTTGAAATATTTAAAGAACACAGATTTTCATTTGATGAAATCAATCAAAAATTATTCTTACAAAATTTAAATATAGAAGACCCTAAAAAACCATTTAAAAAAATAAAATTTGAAGATTTTTATAACAATAGAGTAAAAGATTGGAATTTTAAAGAAAAGTCTGAATACAATAGAGCTTTGGAGTTGGATAATGATCTTAAATATCCCTTCTTTGATATAAAGAATCAAAATAGTTTTATTAATTTATTAAAAAATGATCCCGATCCTAATCTTTATAGGGTTTTTAATAAATATTTTCAAATAAGATTAAGAACAAATGATGAACTTTTAAAAATACTTTTTGTGTTTGAATTAGTATCGAAAAAAATGCAACAACAATTAAGTAATAAAATAAAAAAAGATGCCTCGGATTTAAATGTAAAGAATTATTTCAATTTAAAAACATATGCCATAAATCATTTTTTATTAGATTATACGGAATTTCTAAAATCAGAAGAACCCGAAACTACAATGCATTATACATTTAGACTATTAAGCTATATTGTGCCACCAGTTGCCATTTCTGGCATTATAATTTATATTATTATAATGGTATTAAAAAAGAAGCATAAATCAAAGGCAAGAGCATAA", "aa": "MNKKQIKKFLSFSLAIPTLLTIPLVSLSCKKSKDNQDNLELGNDDGIEKQDLSVAKRIVEIFKEHRFSFDEINQKLFLQNLNIEDPKKPFKKIKFEDFYNNRVKDWNFKEKSEYNRALELDNDLKYPFFDIKNQNSFINLLKNDPDPNLYRVFNKYFQIRLRTNDELLKILFVFELVSKKMQQQLSNKIKKDASDLNVKNYFNLKTYAINHFLLDYTEFLKSEEPETTMHYTFRLLSYIVPPVAISGIIIYIIIMVLKKKHKSKARA", "aa_hexdigest": "9637e67e1290089c8dead73e9c2e9b2a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI000DCB2741", "ncbi_nrp_id": "WP_112636580.1", "uniref100_id": "UniRef100_UPI000DCB2741", "db_xrefs": [ "SO:0001217", "UniParc:UPI000DCB2741", "RefSeq:WP_112636580.1", "UniRef:UniRef100_UPI000DCB2741" ] }, "ips": { "uniref100_id": "UniRef100_UPI000DCB2741", "uniref90_id": "UniRef90_A0A454CA04" }, "psc": { "uniref90_id": "UniRef90_A0A454CA04", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA04" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA04", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA04" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_767", "locus": "GCA013368035_00756" }, { "type": "cds", "sequence": "contig_1", "start": 431500, "stop": 432123, "strand": "+", "frame": 1, "gene": null, "product": "p50 adhesin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103", "UniRef:UniRef90_O69453" ], "nt": "ATGAAAAAATCAAAGAAAATATTTATAACATTATGTGGAATAGCAACTACTGCTATTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCTTTAAAACAAGCAAATGCATTAGCGGAAGAATTGAAAAAAAATCCTGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCCGAAGCAACCAAATCATTTAAAGAAGCAGGTTCATACGGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAGTAGAAAACGCAAAAAGTGAACAACAAAAAGTTGATCAAGCAAATAAAAAAATTGCTGATGAAAATCAAAAAATTAAAGAAGGCGCTAAAGAATTATTAAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTAATTTCAACAATTGAATTATTAAATAAAAAATCAGTCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACAACTCATGA", "aa": "MKKSKKIFITLCGIATTAILPVATISCNDDKLAEKNGKEKADAALKQANALAEELKKNPDYSKILETLNKEIAEATKSFKEAGSYGDYPAIISKLSAAVENAKSEQQKVDQANKKIADENQKIKEGAKELLKLSEKIQSFADTIALTITKLEGKKFQIDETFKKQLISTIELLNKKSVEVKTFATVNTIKKDFLLSELESFKEFNNS", "aa_hexdigest": "bb7963fcc10801af03474bd773ae2aad", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "psc": { "uniref90_id": "UniRef90_O69453", "query_cov": 1.0, "subject_cov": 0.8023255813953488, "identity": 0.981, "score": 339.0, "evalue": 4.31e-116, "valid": true, "product": "p50 adhesin", "uniref50_id": "UniRef50_O53103" }, "pscc": { "uniref50_id": "UniRef50_O53103", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O53103" ], "product": "Vaa surface lipoprotein adhesin" }, "genes": [], "id": "GLJLFKCOHC_768", "locus": "GCA013368035_00757" }, { "type": "cds", "sequence": "contig_1", "start": 432276, "stop": 432431, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTATTTACTGTTGCAAAAGTTTTAACTTCGACTGATTTTTTATTTAATAATTCAATTGTTGAAATTAATTGTTTTTTAAATGTTTCATCAATTTGGAATTTTTTTCCTTCTAATTTAGTAATTGTTAATGCAATTGTATCAGCAAAACTTTGA", "aa": "MVFTVAKVLTSTDFLFNNSIVEINCFLNVSSIWNFFPSNLVIVNAIVSAKL", "aa_hexdigest": "62b0bc510fdc1cefdc808f4ab5af7b1a", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5681.6195, "isoelectric_point": 5.820676612854004 }, "id": "GLJLFKCOHC_769", "locus": "GCA013368035_00758" }, { "type": "cds", "sequence": "contig_1", "start": 432600, "stop": 432845, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTTAATTCTTTAAATGATTCTAATTCTTCAAGAACAAATTCTGTCTTTTTAGAAACAGTAGAAACAAATGTATTGATTTCGTTAGCTTTTTTATTTAATAATTTGATTGTTGAGGTTAATTTTTCTTTAAAATCTTTTGGAATTTGGAATTTCTTTTCTAATTTAGCAATAGTTTCTTCAATAGTTTTTGCTAAATCTGAATTTTCCTTGCTTAATTTTGATAATTCTGCGATACCATTTTGA", "aa": "MFNSLNDSNSSRTNSVFLETVETNVLISLAFLFNNLIVEVNFSLKSFGIWNFFSNLAIVSSIVFAKSEFSLLNFDNSAIPF", "aa_hexdigest": "2c71ba5db44e48669aba69cabff6271a", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9095.20969999999, "isoelectric_point": 4.3588346481323255 }, "id": "GLJLFKCOHC_770", "locus": "GCA013368035_00759" }, { "type": "tRNA", "sequence": "contig_1", "start": 433214, "stop": 433290, "strand": "-", "gene": "trnH", "product": "tRNA-His(gtg)", "amino_acid": "His", "anti_codon": "gtg", "score": 59.6, "nt": "GCGTTTGTGGCGAAGTGGTTTAACGCTCCGGGTTGTGGCTCCGGCATACGTGGGTTCGATCCCCATCAAACGCCCCA", "db_xrefs": [ "SO:0000262" ], "anti_codon_pos": [ 433282, 433284 ], "id": "GLJLFKCOHC_27", "locus": "GCA013368035_00760" }, { "type": "cds", "sequence": "contig_1", "start": 433673, "stop": 433993, "strand": "-", "frame": 1, "gene": "hinT", "product": "histidine triad protein HinT", "db_xrefs": [ "COG:COG0537", "COG:FR", "RefSeq:WP_175949904.1", "SO:0001217", "UniParc:UPI00158F5A41", "UniRef:UniRef100_UPI00158F5A41", "UniRef:UniRef50_P32083", "UniRef:UniRef90_D1J8D6" ], "nt": "ATGCATGATGATATTTTTGAAAAAATAATAACAAGAGAAATCCCAGCAAAAATAATATATGAAGATGAAAAAATTATTGCCTTTGAAGATATTAAACCAGTAGATAAAGGTCATTTTTTAGTAACGGTAAAAAACCACGCCACAAATTTATATGATATTGATGACCAAGCATTGTCATCACTAATATTAAAGGCGAGAGAACTTGCTTTAAAAAGAGTTAAGGAATTAGGTGCAACCGGATTTAATTTAATAATAAATAATAATCTTTCAGCAGATCAAACAGTATTCAGAATTCATGTTCATATAATCCCAAGATATTAA", "aa": "MHDDIFEKIITREIPAKIIYEDEKIIAFEDIKPVDKGHFLVTVKNHATNLYDIDDQALSSLILKARELALKRVKELGATGFNLIINNNLSADQTVFRIHVHIIPRY", "aa_hexdigest": "e967d744b5b0ec62590e6a5b17f0b7c1", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00158F5A41", "ncbi_nrp_id": "WP_175949904.1", "uniref100_id": "UniRef100_UPI00158F5A41", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158F5A41", "RefSeq:WP_175949904.1", "UniRef:UniRef100_UPI00158F5A41" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158F5A41", "uniref90_id": "UniRef90_D1J8D6" }, "psc": { "uniref90_id": "UniRef90_D1J8D6", "gene": "hinT", "product": "histidine triad protein HinT", "uniref50_id": "UniRef50_P32083", "cog_id": "COG0537", "cog_category": "FR" }, "pscc": { "uniref50_id": "UniRef50_P32083", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P32083" ], "product": "Uncharacterized 13.1 kDa HIT-like protein in P37 5'region" }, "genes": [ "hinT" ], "id": "GLJLFKCOHC_771", "locus": "GCA013368035_00761" }, { "type": "cds", "sequence": "contig_1", "start": 433995, "stop": 434123, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGACTGCTAATTTTGAGACATTATACAAAACTGCAGTTAAATATATTAATGAATTAGGGTTTAAATTAAATGTTTCAGAAACAAACAAATTTGTTTATGATATATTAAAAGTTGAAGGTCTAGTATAA", "aa": "MTANFETLYKTAVKYINELGFKLNVSETNKFVYDILKVEGLV", "aa_hexdigest": "f3deca90260ed27829227fea379183cb", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4844.577100000001, "isoelectric_point": 6.0701997756958 }, "id": "GLJLFKCOHC_772", "locus": "GCA013368035_00762" }, { "type": "cds", "sequence": "contig_1", "start": 434163, "stop": 434858, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGGCGAAGTATTAAAATCATTATTGGCATATAAAGGCATAGTTAAAAATTCAAATACGTCAGGTGATTTAGGTGGCAATTTAGATAGTATAAAGAAAGATTTAAGTTCAGTATATGGATTTGTAGATCCATACAGCAAAAAAGTTTATAGTCAAGAAAGCTTTTTACTTGCAAAAATTCTTGCCCAAGAAATAAACCATCCAAAGGCAAAAGCAACCGAAACATTGCAATCTAAAGTTTCAAAAGGTGAATTAAAATCGTTTGATTACAAAGATTACGAATTTGATGGTTTAACAAAAGATAGCAAGGATAATTATCAATATACAAAGACAATAACTCTTGGTAGTAAGCAATATACATTGAGATTTAGTCAAAAAGGTTCAATATCATTTGATGGAAACTTTTTAACAATTCCTATGAATTTAACAGTTGATGGTTTAGGAAAAAGAAATTTCTATGAATTCAATGCTAAACTAGAATACAATAAAAATACAAAGAAATTTGATTCAATAAACCAAGATGTTGCATACAATTTAAAACAAAATCCTCAAAAAATAAATGTTACAAAAGACAATTCAATTACTGCCCAATATGTAGTTAAAATTTCACCATTATATTTAACTAAGAAAGTTAAAGATTATAACGGCAAAGAAGTTACAAAACAAGTTTTAACATTTGATGAAACTCCATGA", "aa": "MEGEVLKSLLAYKGIVKNSNTSGDLGGNLDSIKKDLSSVYGFVDPYSKKVYSQESFLLAKILAQEINHPKAKATETLQSKVSKGELKSFDYKDYEFDGLTKDSKDNYQYTKTITLGSKQYTLRFSQKGSISFDGNFLTIPMNLTVDGLGKRNFYEFNAKLEYNKNTKKFDSINQDVAYNLKQNPQKINVTKDNSITAQYVVKISPLYLTKKVKDYNGKEVTKQVLTFDETP", "aa_hexdigest": "e74ad4116fbc5272ce8bad9ce19376b6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26169.31120000002, "isoelectric_point": 9.23253345489502 }, "id": "GLJLFKCOHC_773", "locus": "GCA013368035_00763" }, { "type": "cds", "sequence": "contig_1", "start": 435021, "stop": 435377, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATCAATGCAAAGATTGACACAACTAACTATAATCCTGCTGCATTTTCAATACCAAATGAACAAGAATTTAAATTTATTTTTAAACACTCAAATCAAATCGCGGCAAACATTAGATTAGAACTTCAAAAAATGTTACTTGCTAAACTTTATTTATTAAAAAATAGACCAGAACTTAAAAAAATAGCAAATGATAGCAACGGTCTAGACAAAGCACAAGTTGCATTACACAACAAAATGTCTAAGAAAGATGCTCCTATTAATGAAAAAGAATTATATGAAGCATTGAATTTCGCAGATGATTCATTGTATTTAATGAAATATCTAGTTGAAAATCCGATTATTGAAAATTGA", "aa": "MFINAKIDTTNYNPAAFSIPNEQEFKFIFKHSNQIAANIRLELQKMLLAKLYLLKNRPELKKIANDSNGLDKAQVALHNKMSKKDAPINEKELYEALNFADDSLYLMKYLVENPIIEN", "aa_hexdigest": "a47dd4e8121b00def68312f73452e280", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13645.632299999981, "isoelectric_point": 7.883854866027832 }, "id": "GLJLFKCOHC_774", "locus": "GCA013368035_00764" }, { "type": "cds", "sequence": "contig_1", "start": 435579, "stop": 435743, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTAAAAAAATTAGATTATTAACAGCTATTGCAATGCCACTTGCGGTTCTTCCTTTGATTGCTAGTTCATGTAAAAAAGAAAAGGAAGATTCTCAACAAAATTCGGGGTACCAAAAACGTGTTTTAAAAGATTCATTAACAAAAAATAGAGTTTTAACTTGA", "aa": "MTKKIRLLTAIAMPLAVLPLIASSCKKEKEDSQQNSGYQKRVLKDSLTKNRVLT", "aa_hexdigest": "96c489145654e415ec9ec61e1a5af3c0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6059.176500000003, "isoelectric_point": 10.103952598571777 }, "id": "GLJLFKCOHC_775", "locus": "GCA013368035_00765" }, { "type": "cds", "sequence": "contig_1", "start": 435755, "stop": 436741, "strand": "-", "frame": 1, "gene": null, "product": "HinT-interacting membrane complex protein P80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "nt": "TTGAGATATGTTTCAAGTGATAGCAAGTCAGCTGATAAATATGGTTTCGACGGCTTTAAAAGTGTATTGAATAGTTTAAAAGAAAAAGATCCTAATAAGTATATGAATCTTCTTTCGATCTTAATGGGAGATGCTAACAAAGATAAAAGCATTTTTAAAGTTGAAGATAAAAAAGATTTGTTTACAAAATTAAAAGAAAACTTCAAAAAGATCTTTGGCGATGCCACATTTATTGATCATGAAAAGAAACCAAAGAAATTTGCTGATATTTTTAATGAAGGAACTGAAAGTGTTGACAAAGAAAATTTCTCTGAAAAATACACTAGATACAATGCAGCTATTGAAAAATTTATCAATGATTTAAGTGAAAAAGATTTTAATAAATCTTTTGGTTTGGCATTTCGAGATGCATTTAGTGATGAAGCAAGTGGGTATAAGGTTTCAACATTAATTAAAAGTAATGGAAATTACATTCAAGTTAATGAAAGTGGCATTTTAATTCAAAATGTATTTTCATTAGACAAAGAAGAAGTTGTAACTAGATTGATAACAAACGATTTGTCAATACTATCTAAGGCTAACCACTCAGATACATTAAATACCAAATTGTTTAATTTAGATTCAATATTTTCTGAAATATTAACCCGTGACTATATGGTTAATGATTTATTACAACAAGATAATTTTAAAAATTATATTAAATCAAAAGAATATTTAAGTCTTGAAGGAACAAAACGTAAATTCAACGATCAAGATATTTTAAATGCAATCAACTATGAAAAATTAATTCGCGAAACAAGTAAATACGTTTTAATTAATGGCAAAGCAAACGAAATAAAACAATTTATAATTAAAAAATTTAATAGTAATTTATACAAAGATTATGTGTTCAAGAACAATAAGTTTATTCTATCACCACATGAAGACAAAGAGGTCTTATCTTATTTATTCACAACAATTGTAAATTACATAAATACTAAAAATTAA", "aa": "MRYVSSDSKSADKYGFDGFKSVLNSLKEKDPNKYMNLLSILMGDANKDKSIFKVEDKKDLFTKLKENFKKIFGDATFIDHEKKPKKFADIFNEGTESVDKENFSEKYTRYNAAIEKFINDLSEKDFNKSFGLAFRDAFSDEASGYKVSTLIKSNGNYIQVNESGILIQNVFSLDKEEVVTRLITNDLSILSKANHSDTLNTKLFNLDSIFSEILTRDYMVNDLLQQDNFKNYIKSKEYLSLEGTKRKFNDQDILNAINYEKLIRETSKYVLINGKANEIKQFIIKKFNSNLYKDYVFKNNKFILSPHEDKEVLSYLFTTIVNYINTKN", "aa_hexdigest": "646c5e02841364017fd2fb0e0e7105bb", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021BDBC09", "query_cov": 0.9298780487804879, "subject_cov": 0.9967320261437909, "identity": 0.528, "score": 293.0, "evalue": 2.47e-95, "valid": false, "product": "HinT-interacting membrane complex protein P80", "uniref50_id": "UniRef50_UPI0021BDBC09" }, "pscc": { "uniref50_id": "UniRef50_UPI0021BDBC09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021BDBC09" ], "product": "HinT-interacting membrane complex protein P80" }, "genes": [], "id": "GLJLFKCOHC_776", "locus": "GCA013368035_00766" }, { "type": "cds", "sequence": "contig_1", "start": 437555, "stop": 437677, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAACTATGGTGATATCAATAAAATCCATGAAATAGCAAATTCATCAAAACATGCTGCTGAAGGTGTTGAAAAGCAACTAATTAAATACCTTTATGAAAAAGAATATGAAGGTTCATTGTGA", "aa": "MNYGDINKIHEIANSSKHAAEGVEKQLIKYLYEKEYEGSL", "aa_hexdigest": "fc4f3f946ea3a21e1f35b177f91b4cf9", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4614.1062999999995, "isoelectric_point": 5.503799247741698 }, "id": "GLJLFKCOHC_777", "locus": "GCA013368035_00767" }, { "type": "cds", "sequence": "contig_1", "start": 437997, "stop": 438410, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5", "UniRef:UniRef90_UPI001F2617A5" ], "nt": "ATGAATAAATTACTTTTAAATAAGGATAGAATATTGCTTGAATTTATTGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTGAGCTTGTATGGCGCTATGTCAAAAGAACATTCTTGGAATAATTTAGATCACCATGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTGCATTCTTTGTATCACGACTTACAAATAAAATTTGTTGATGATAACAAAAATATATTTGAAAACCAACCGTTTTTGAATATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATCGCAATCAAGCTAGATCGTTTATTGGCCACTATTTAACAATGACAAATAGCAACGATTGA", "aa": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGAMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTNIANIKKYLKHRNQARSFIGHYLTMTNSND", "aa_hexdigest": "1b803523a63b08ef94e0317a35caa166", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 437997, "stop": 438522, "observations": { "insertion": [ 438498 ], "mutation": [ 438408 ], "stop": [ 438408 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F2617A5", "query_cov": 1.0, "subject_cov": 0.7873563218390804, "identity": 0.978, "score": 274.0, "evalue": 1.1e-92, "gene_start": 1, "gene_end": 137, "reference_start": 1, "reference_end": 137, "reference_sequence": "MNKLLLNKDRILLEFIEICKNLNLFYSLDKLSLYGTMSKEHSWNNLDHHEIMMKYQDYEILKNACPNRVLDNTLHSLYHDLQIKFVDDNKNIFENQPFLNINLIYPTSIANIKKYLKHCNQARSFIGHYLTMTNSNDWKTKNNIIKSHFLSMFYKPITYKEVANLLIKMITKVF" }, "paralog": false, "identity": 0.9712643678160919, "score": 341.0, "evalue": 1.08e-121, "description": "Internal stop codon at 438408. Frameshift due to insertion around 438498. Nonsense mutation around 438408.", "psc": { "uniref90_id": "UniRef90_UPI001F2617A5", "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F2617A5", "UniRef:UniRef50_UPI001F2617A5" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F2617A5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F2617A5" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 16391.66339999998, "isoelectric_point": 7.8015932083129895 }, "id": "GLJLFKCOHC_778", "locus": "GCA013368035_00768" }, { "type": "cds", "sequence": "contig_1", "start": 438564, "stop": 438743, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD", "UniRef:UniRef90_UPI001F176BBD" ], "nt": "TTGCCAAATATTAAATTTGAAACAAAAAAAGTAATTATAAACAATATTGAAGTTGATGTTTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATTCCGTTCCACAAAGAAAGATGGAATATATTAACCCCATTGAATATAAAAAAATATTTAGCTAA", "aa": "MPNIKFETKKVIINNIEVDVLVEFDQYLKNVYEVEYFEHSVPQRKMEYINPIEYKKIFS", "aa_hexdigest": "9d8fb05ae7d4fe8a3545620af0159f56", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001F176BBD", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.983, "score": 116.0, "evalue": 2.79e-33, "valid": true, "uniref50_id": "UniRef50_UPI001F176BBD" }, "pscc": { "uniref50_id": "UniRef50_UPI001F176BBD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F176BBD" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7244.2973, "isoelectric_point": 5.270873069763183 }, "id": "GLJLFKCOHC_779", "locus": "GCA013368035_00769" }, { "type": "cds", "sequence": "contig_1", "start": 438901, "stop": 439845, "strand": "-", "frame": 2, "gene": "lolD", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1136", "COG:M", "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2", "UniRef:UniRef90_D1J8E0" ], "nt": "ATGGCAAAAGAAATAAAAGAAAACAAAAGAGAAATTAAAGCTAAAACAAATAAAATTTCTAAGAATAAAAATTTTGTTGATGAACAAACATTGCAAGATTTGAACTTGATAAATATTTCTAAGCAAAAAAAGCCATTTGAAATTAAAGACGAAGGCATTGCAATTAAGGTTTTAGATAATGCAACTTTAAAGAAATATAGACTTGCTAATGACAAACCAAGAAAAAAAGACGAGTATGCTAAATTGCAAAATTCTAACGATAACATTGTTGAAGTTGATAATATTAAAAAAACATATTTATCCGGCAATGTAGCAACTGAAGTTTTAAAAGGAATAACTTTTTCAATTAAAAAAGGAGAAATTGTTGTTCTATATGGAAAGTCTGGGTCAGGTAAATCAACATTATTAAACATAATTAGTGCTCTTGACCGGCCTACCTCTGGCAATGTAATTGTAAATAACATAAATTTGCCTTATTTATCTGATGCAAAGCAAACTTTATTTAGAAGAGAAAATATTTCGTTCATTTTTCAAAATTATAATTTACTTCAAAATTTGAATAGTTATGACAATGTTGAAACCGGTTCATATTTACAAAGAGATAAAAATAAGCGTTTAAATATTAAAAAATTATTTGAAGATTTTGATCTTTCTGAATGTATGTATAAATATCCTTCTCAAATGTCCGGTGGTCAACAACAAAGAGTGTCAATTTTAAGAGCCATTGCAAAAAATTCCGAAATTCTTGTAGCTGATGAACCAACAGGAGCATTGGATGAAAAAACAGGAATTATTGTATTGAAAAATTTGCAACAAATCAATTTAAAATATGGCACAACCATTATCATTGTTTCCCACGACCCTGACGTTGCCCAAATTGCTGATAAAGTTATATACTTGGAGTTGGGATTAATTAAAGAAGTAAAACTTCAAGAAAGAAAATGA", "aa": "MAKEIKENKREIKAKTNKISKNKNFVDEQTLQDLNLINISKQKKPFEIKDEGIAIKVLDNATLKKYRLANDKPRKKDEYAKLQNSNDNIVEVDNIKKTYLSGNVATEVLKGITFSIKKGEIVVLYGKSGSGKSTLLNIISALDRPTSGNVIVNNINLPYLSDAKQTLFRRENISFIFQNYNLLQNLNSYDNVETGSYLQRDKNKRLNIKKLFEDFDLSECMYKYPSQMSGGQQQRVSILRAIAKNSEILVADEPTGALDEKTGIIVLKNLQQINLKYGTTIIIVSHDPDVAQIADKVIYLELGLIKEVKLQERK", "aa_hexdigest": "9ea6ff1ce3110b78c1c2138a7385210f", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8E0", "query_cov": 1.0, "subject_cov": 0.9721362229102167, "identity": 1.0, "score": 588.0, "evalue": 9.11e-212, "valid": true, "gene": "LolD", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A448ZXQ2", "cog_id": "COG1136", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "lolD" ], "id": "GLJLFKCOHC_780", "locus": "GCA013368035_00770" }, { "type": "cds", "sequence": "contig_1", "start": 439895, "stop": 440575, "strand": "-", "frame": 1, "gene": null, "product": "FtsX-like permease family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702", "UniRef:UniRef90_UPI001F16D702" ], "nt": "ATGTTTATAAGCAATCCAAAATTGAAATATATTTCTGTATATGAACATAACATTCAATCATATAATGAAGCACATCCTCAGGCAAAATTAGATTATAAAACTCAATTAAAGAAATTACTTGGCAATCTAAACGATGAAAACATACAAGAAATAATAGACTCAGATAGCCCATTAGAAAGTTATAAAATCTTAGTAAGAGAAACATTAACTAAATTCTATGGCACACAAGCATCTTCAATATATGGCAAGAATATAATGTATGGTGCATCATTTGATGTCAATAGTAAAGACATTGAAGCTGGATTTATTGAAGGAATTTCTTCAACAGTTAATACCATATTGCTGGCATTTATAATAATTTCATTGATAATTTCAATAGTTATATTAATTGTTATTACTAATATAATGATTGCTTCAAATGGAAGATCAATTGCTACATTTTCTATATTAGGATATACAAATAAAGAAAAAATTCTATTATTCTTCTTCAATTTTGTACCTGCAATTGTATTTGCTTGCTTACTAATGATTCCTGCAACTCTTGCAATAATAGCAATATTTAACTCGTTTATGATGGTAACTAGCCAGATTGTATTACCTTTGGTTTTACATATTTCAACAGTAATATTAAGTGCATTAATTTGTTTGGCAGTATTTATAGCAACATCAATTGCAACATGA", "aa": "MFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDENIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIAT", "aa_hexdigest": "2b92e99dbd8665289a00b00c739ae80e", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "genes": [], "truncated": "both", "pseudogene": { "start": 439847, "stop": 440710, "observations": { "mutation": [ 439897, 440593, 440644 ], "stop": [ 439897, 440593, 440644 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001F16D702", "query_cov": 1.0, "subject_cov": 0.7874564459930313, "identity": 1.0, "score": 387.0, "evalue": 3.37e-134, "gene_start": 1, "gene_end": 226, "reference_start": 46, "reference_end": 271, "reference_sequence": "MSIDKVPVQTIDTLTTYSATGFWGAASSYDVAEANDNSAWDFFKRMFISNPKLKYISVYEHNIQSYNEAHPQAKLDYKTQLKKLLGNLNDENIQEIIDSDSPLESYKILVRETLTKFYGTQASSIYGKNIMYGASFDVNSKDIEAGFIEGISSTVNTILLAFIIISLIISIVILIVITNIMIASNGRSIATFSILGYTNKEKILLFFFNFVPAIVFACLLMIPATLAIIAIFNSFMMVTSQIVLPLVLHISTVILSALICLAVFIATSIATWNSLNKIKAVDALKGK" }, "paralog": false, "identity": 0.9860627177700348, "score": 515.0, "evalue": 3.13e-187, "description": "Internal stop codon at 439897, 440593, 440644. Nonsense mutation around 439897, 440593, 440644.", "psc": { "uniref90_id": "UniRef90_UPI001F16D702", "product": "FtsX-like permease family protein", "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001F16D702", "UniRef:UniRef50_UPI001F16D702" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001F16D702", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F16D702" ], "product": "FtsX-like permease family protein" } }, "id": "GLJLFKCOHC_781", "locus": "GCA013368035_00771" }, { "type": "cds", "sequence": "contig_1", "start": 440642, "stop": 441145, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGATTGCAAGATAGTCGGGGCAATAATTTAACAAAATTATTACAAGACTATAATTACAATTTTGATAGTAATGATCCAATTCCTGTTGTTGTAAATGAAGTAACGGCAAGAAGCTATAGATTAAAAGTTAATTCCGTATTTGATGCCAAAATATTAAATCACGTTGATCGATTTGCATATCAAGCTTTAATGCAAAAAAGCCCAAGAGTCGATTATAAGTTTAAAGTAATTGGCATATCAGATACTTATATTAACTCTGAAATTAGTACAAGGAAGGATATTTTAGACCACATTTTAGGCTATGATACTCTTTCAAAAAGATTAAAAGATGCAAGAAAATACGAATTACAAAAAGCAATTAGTTTACACCCTAATTTAAAAGATGAATATATTAAAAAATTCAATAAAAAATATGATGCATTTAATGGTATTTTATCAATTGATAAAGTACCAGTTCAAACTATTGATACATTAACAACTTATTCAGCAACAGGATTCTGA", "aa": "MRLQDSRGNNLTKLLQDYNYNFDSNDPIPVVVNEVTARSYRLKVNSVFDAKILNHVDRFAYQALMQKSPRVDYKFKVIGISDTYINSEISTRKDILDHILGYDTLSKRLKDARKYELQKAISLHPNLKDEYIKKFNKKYDAFNGILSIDKVPVQTIDTLTTYSATGF", "aa_hexdigest": "435b58e6401b3967f66c368acbcad56a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 19364.841900000003, "isoelectric_point": 9.3358118057251 }, "id": "GLJLFKCOHC_782", "locus": "GCA013368035_00772" }, { "type": "cds", "sequence": "contig_1", "start": 441380, "stop": 441688, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTGAAACACAAAGGGCAAGAGTAAACCAAATATTTAACCGCGTAAGTTCAATGATGCAATCAACTCAAAACTTAATAAATTTACGTAGATTGAAAAATGGTGGAGATTATAGAATAATAGATGAAGAAGATGTAAAAGATCCAACAAAAATAATTGCTGTAAAAGATATTAAAAAATTCTTTGATGACTATAATGCTAAACGTCCAGAAGATTTATCAAATAGAACAAGTTACTTCTTGTTTAGCGCTGGTAATTATTCAGAAGCAGCATCAGGAAATAGAAAACAATTTAGATTTGTTGAATGA", "aa": "MPETQRARVNQIFNRVSSMMQSTQNLINLRRLKNGGDYRIIDEEDVKDPTKIIAVKDIKKFFDDYNAKRPEDLSNRTSYFLFSAGNYSEAASGNRKQFRFVE", "aa_hexdigest": "6b08e1a730bc0aa42ee5b39d2731b77b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11945.299099999987, "isoelectric_point": 9.51716136932373 }, "id": "GLJLFKCOHC_783", "locus": "GCA013368035_00773" }, { "type": "cds", "sequence": "contig_1", "start": 441707, "stop": 442093, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTCGTTATTCTTAGCATTTTCAACGACTTCATTAATTTCTATGTTCTTCTTATCTTCAAATAACGTATTTAATAAAACTATTTCAAAGACATATAAAAATAGGCTATATAAATATAAATTAAATCTTGAAAGTCCAACAACCGAAGGTGGTCCTTATGTTACATACAATCAAAACGATATAAATGATTTATTGTATGTTCCTAATGATCTTGCAGGTGATTCATCAACAAATGGTAGTCAACTAGATTATGATAATCCTAATTTCTTAAGACCTGGAGCTTCATTTAATACAGATGTAATTCAAAGAAAATTTGCGCCTACTGTCATTACAAAATCATCGCTTGATTTATTAATGGATTTATCAGTTGAATTAAGTCCATGA", "aa": "MFSLFLAFSTTSLISMFFLSSNNVFNKTISKTYKNRLYKYKLNLESPTTEGGPYVTYNQNDINDLLYVPNDLAGDSSTNGSQLDYDNPNFLRPGASFNTDVIQRKFAPTVITKSSLDLLMDLSVELSP", "aa_hexdigest": "81bfa1e11e916d49b86f73582905571f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14374.00049999998, "isoelectric_point": 4.882492256164551 }, "id": "GLJLFKCOHC_784", "locus": "GCA013368035_00774" }, { "type": "cds", "sequence": "contig_1", "start": 442097, "stop": 442282, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTGTTGTTTCACTAGTTATTTATATAATTACTAGAGTTATTGTAAGAAATAAACCTACAGAATTAATGAGTGGGTTAACTGAAGTTAGTGTTGGTAATTTTGCCCAAAGAGTTTCTGCTATGTTTAGAAAAATGCCAGTTAAATCAAGATTTATTGCCTCGATGGCATTGAATAATTTCTGA", "aa": "MIVVSLVIYIITRVIVRNKPTELMSGLTEVSVGNFAQRVSAMFRKMPVKSRFIASMALNNF", "aa_hexdigest": "b8c80b17c836aff5f20138501a276960", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6876.251800000002, "isoelectric_point": 11.466427421569826 }, "id": "GLJLFKCOHC_785", "locus": "GCA013368035_00775" }, { "type": "cds", "sequence": "contig_1", "start": 442433, "stop": 444034, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGGTCTTTACAACATAGGAACATCAAAAGAAATTAAAGATATAGTATTAAATTACAAAAAAGTAATTAACCCAATTCCTAGTCTTGGAATTTTAGGCTCGGACGATGACTATTCTGCTAAATTATTTAAAATGCCTTTTGCTCACGCGCATACAGCAAAAGCAACAAAGCAAATTATGGATTTAATGAATAATTCGGCAGTATTTAAAAATTTATTTGACCAATTATCTAAAAATCATGTTTCTAATAAGGGCATTGAAGCAATTAAAGAGATATTAAAAAATAATATGAATGAAATAACCTACAATTTTGGTTATATGGCCTCTTCAAATCAAATGCCAACTCATTATCTTCAAGCCTTAAAATTATTTTTACAATCATTTATTAATGTCAATGATCAAAATCAAAAAATTAAACCGTTAATAAATGATGAATATACCTTTAGCTTAGTATATAAGGATGCAATTGCAGCTTCAAAAACCGCCTCAATAATTTCGTTGTTAAACGTACCAAGAAATATTCTAAATGGTTTCAATATTTTAAGTTTCCCACAAATATTGTTATACTACGTATTAAGTCCAAACCCAGATAGTGGAAATATTGCTTATATAATCAAAAAATTATTATCGAATATTGATGAAACTAATGCAACAATGTTGAAAAAACAAATCGAAGGTGCAACTCAAAAATTCAATTTTGTTGAAAATGTTGTTACTTCAAAAAGCGATAGCAGAATTGATTTAGATATTTCTAAATTCAATTATATGTTTAACCATTTATTAAAAACACCCGATAACAAAGATATTAGCTTTTTTGGAATAAATATTACCAAAACTGTTAAGGAAGTATTTTCAAAAATAATTGAACCAAGCACAGTTTATAATTTAATTGCATATTCCGAAGCTGCTTCATATTTAGCCAAGGTAAATTATGGATATTTAAACAAAAATAAAAAAGAAGTATACAATGGCGATATTTCGAAATATTTAAATAATCCATTTGAAATGCAACAATTTATTGCTTCATTAGATGATAAATATAAAATTAAAGTAAATACCCAAGAATATTTGATAACAGGTATTGATAGCACCGCTGATTATTTATACCCTGTAATTAATGAAGAAAATTTACAAGTTGATACAAAAACTCAATCTGTTGTTTATGTTAATTCAAGAGGTTTTGACAGAATTAGATCGGCATATCCTACATTTGCCTTAAAGAGTTATGCATTAGTAAAATCTCCTGTTGACAAAAAAGGAAAATATTTAGCTGGCAAAGATCCTAAAAACTTAAAGAAAGAACTTCAAAGAATTGTTTCATCAATTTCTCCAAATTCAAAAAATAAAGTATTTTTAAAAGATGAACCAGATGCAATCAACCCTGAACGTTACATCAGAGTAAAAACAATAAGAACATTAGTAACATCAATTAAAAATGCAACACTTTATTTAATAACAATTTTGGCAATATTAGTAATTTTGATTGTTTACTTTATTATCAAAAGATATATTGAAGCAAGAAATAAGGTTGTTGGAATATTAAGAGCTCAAGGATATAAAACATCAGAAATTGCTTTATCATTCTGTGCTTTTGGTTGA", "aa": "MLGLYNIGTSKEIKDIVLNYKKVINPIPSLGILGSDDDYSAKLFKMPFAHAHTAKATKQIMDLMNNSAVFKNLFDQLSKNHVSNKGIEAIKEILKNNMNEITYNFGYMASSNQMPTHYLQALKLFLQSFINVNDQNQKIKPLINDEYTFSLVYKDAIAASKTASIISLLNVPRNILNGFNILSFPQILLYYVLSPNPDSGNIAYIIKKLLSNIDETNATMLKKQIEGATQKFNFVENVVTSKSDSRIDLDISKFNYMFNHLLKTPDNKDISFFGINITKTVKEVFSKIIEPSTVYNLIAYSEAASYLAKVNYGYLNKNKKEVYNGDISKYLNNPFEMQQFIASLDDKYKIKVNTQEYLITGIDSTADYLYPVINEENLQVDTKTQSVVYVNSRGFDRIRSAYPTFALKSYALVKSPVDKKGKYLAGKDPKNLKKELQRIVSSISPNSKNKVFLKDEPDAINPERYIRVKTIRTLVTSIKNATLYLITILAILVILIVYFIIKRYIEARNKVVGILRAQGYKTSEIALSFCAFG", "aa_hexdigest": "157b6d298458f4e6602c440cdfcedc5f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 60487.295600000034, "isoelectric_point": 9.417751121520997 }, "id": "GLJLFKCOHC_786", "locus": "GCA013368035_00776" }, { "type": "cds", "sequence": "contig_1", "start": 444167, "stop": 444961, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAGCAACTAGTTAAACATGAACATTATCCAATTGTTGCAAATAATAAAATTTATAAAGATTTCAACACAGAAAGTTTAACTAGCTCAGATTATTTAACATCATTTTTTGGTGCAATAAGTTCAAACCTAGATGGAAAAATAAATAGCGGAAAAATTTCCCAAATTCAAGAATCCGTTATTCAAATGTTTAATTTTTCAAATAAGACAAAAAGAATTTTAGGTAAATTGAATATAACTATTCCTGATTATGACAATAATAAAATTACTTTGTTTGATTTAATAAACATGTCAAAATTTTCATTCCCTCAATCTCAAAAGCAAACAAATGTATTGACTAATATTCCAATAAATCCTTTTGATGTTGATGATATTAAAAAAATATTATTAAAGGCAAAAGATGCAAAAATAAATAACAAAAAGTTGAAATTAACTCAAAACGAATTTGATGTATTAAAAAACAATGTTTTAGTTACTAAAGGCGAATTTAATGACCTTGATTTAATAATTTCAAAATTAACTAAATATCATGAATATATAGACAAACTTGTAACATCAAACTCTCAATATAAGCAAAGCGGTATAAATTCAGCAGTTGAATTAAATGATTTAAAATCTTATTTGGATGTTGCATATTATTCAGCTTCATTTAATAATGTAGCGCCTGAACATGCAAAAGATTTGGAAATTTTAAAAACTATTCATTCATTAATAAAAGAAAATTTAGCAAAATTAATGTTGAATAATGATCCACAAAATGTTGCTTCTAATGTCTTATCTCTATATTCATTATGA", "aa": "MEQLVKHEHYPIVANNKIYKDFNTESLTSSDYLTSFFGAISSNLDGKINSGKISQIQESVIQMFNFSNKTKRILGKLNITIPDYDNNKITLFDLINMSKFSFPQSQKQTNVLTNIPINPFDVDDIKKILLKAKDAKINNKKLKLTQNEFDVLKNNVLVTKGEFNDLDLIISKLTKYHEYIDKLVTSNSQYKQSGINSAVELNDLKSYLDVAYYSASFNNVAPEHAKDLEILKTIHSLIKENLAKLMLNNDPQNVASNVLSLYSL", "aa_hexdigest": "6d0181c416b5aae4d9e680141cae9ac9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 30021.92400000005, "isoelectric_point": 8.422294807434085 }, "id": "GLJLFKCOHC_787", "locus": "GCA013368035_00777" }, { "type": "cds", "sequence": "contig_1", "start": 445334, "stop": 445939, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCTATAAGTTTCGGGGCAATAAACAACAATTTAACCAACGAAATTATTCAAAATAAAACAATAAATGGTTTAGTTAATGTTATTAGAGATGCTTTATTCAAAAGCGAAATTATTAACATGTTCAATAAAAATGATTTATTAAGAATCATGGACGCAGCAAAACAAAGTGTTGTGGATAACGATTTCCATACATTGCTAAATATTGGCAAGGTAAATGGCACGATTTTGCAAAAAGTTCTTTTAGATATTTTGAAATATATTATCAGACCGGTAGATAGAAGCAATAGCAATAATTTAAATATTAATAATGTTAATGTTAATGCTTTTGTTCAAAATGTATTCATTGGAATAATAAACTATTTAGAAAAAATGTATATGAATTCAGGAACCAATGAAAATGAACGAAATCAATATTTAATGCAACAAGTTTACAATATAAGTAAATTATTAAATCTTGATAATCTATATTTGATTCCTTCTTTGAAATTAAGTTTATTTGACATAATTAATTTAGTTAAAAATAAATCAACAATTTTTGAAGTATTGAGAAATGTTATTCGCTCTGTTGATTTTGAAAAATTTTCTTTAATTATTCAAGATTGA", "aa": "MPISFGAINNNLTNEIIQNKTINGLVNVIRDALFKSEIINMFNKNDLLRIMDAAKQSVVDNDFHTLLNIGKVNGTILQKVLLDILKYIIRPVDRSNSNNLNINNVNVNAFVQNVFIGIINYLEKMYMNSGTNENERNQYLMQQVYNISKLLNLDNLYLIPSLKLSLFDIINLVKNKSTIFEVLRNVIRSVDFEKFSLIIQD", "aa_hexdigest": "b01e4201482f3451901122fb7351e5a3", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23110.553900000017, "isoelectric_point": 8.751921653747559 }, "id": "GLJLFKCOHC_788", "locus": "GCA013368035_00778" }, { "type": "cds", "sequence": "contig_1", "start": 446084, "stop": 446263, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAAACACAAAAATACCTTATGAAATCGATGGAAAAATATTGATGTTGACTGTTGCTAATGATGAACCAAAGACCGTTGGTTCTTCAATAGTTGGTGCAGTTGCAAGAATTAGTGATGAAAAATACATTTTATTAAAATATTCAACTATTGATGGATTTAGTGATAAAAAAGTTTGA", "aa": "MPNTKIPYEIDGKILMLTVANDEPKTVGSSIVGAVARISDEKYILLKYSTIDGFSDKKV", "aa_hexdigest": "be2686358a6ebaa3cc9d5e47637ab526", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6474.4519, "isoelectric_point": 6.099187660217283 }, "id": "GLJLFKCOHC_789", "locus": "GCA013368035_00779" }, { "type": "cds", "sequence": "contig_1", "start": 447143, "stop": 447424, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCACACTATTGATGGTAAAGATGTAATGAAGATGCTTGGTTTTAAAGAAATTTCAGAAAACCATTATGAATTTGATGCATCTCTTTATAATCAAAATTCTGATTTAAAAATTGAAAATTCAGATTTTACAAGTCAAAAATCAAAATTTATTAATGAAGACTTTAATATTTCAAGAACTTTTAAACTTCAAAATTTTTTAAGCAATAAAAGTATTGAAAAAGATGTTAATGAATTTACAAAACTAGCAGCAAATAAAATATATCAAATTCCAAATGAATGA", "aa": "MHTIDGKDVMKMLGFKEISENHYEFDASLYNQNSDLKIENSDFTSQKSKFINEDFNISRTFKLQNFLSNKSIEKDVNEFTKLAANKIYQIPNE", "aa_hexdigest": "d5d238aa2fdb70c14d0d92acb282a3e2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10920.073299999987, "isoelectric_point": 5.357438850402832 }, "id": "GLJLFKCOHC_790", "locus": "GCA013368035_00780" }, { "type": "cds", "sequence": "contig_1", "start": 447440, "stop": 448222, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAGACTTTTTAAAGAGGTTTTCAAATCATTGGCAAGAAATAAAGTTACCTTGATTTGTTTAACAATTTTAATTTTCTTAACAACATTTTTATTTACTTTGTTAAATGATGTTACTACTAGCTATTCATCTACGATTAATTCGTATGAAAAAGTTTCAAAATTGCATGATGCAACTGTTGATTTTGACGTTAATCCTTCAGGTGTGATACCAAAATCAGGTTATAACCAAATAGGATCTGATAATCAATCAATTGTTGATAAACCTGTTGTTTTTGAGTCTTCTAATAATGGAAGTGATATTTCATATTCAATAGATCTACCAAAAGATCAAAGAAATTATGTAGGTTTAAAAAATACTTTTAATAATTTAAATTATGATAATGAAAACCATTTTATAAATACTTATGATTTATTAAATTTATTATATTCTAGCAAGGAATCTATAAGTGATGTAGACGTTGAAATTTTAAAATCCAACCCTGATTTTTCTAAAATTAGGAATTTTAGTTTTTCGGGTCATGATAGGGAATTTAAACTTTATGTAAAAGATAACAAAGGAAATATAAAACCATTAACTAAAAGATATAATTTGACAAAAAATGATGACATTACTTTTTTAAATGAAGTTAGATTAAAAAATATAGCTTCTATTGCATATGGTCCAAGAGGCTCGTTAAACGATCAAAAAGTAGTTGATTATTTATACAATCCCTCTCCATTATTTCTAAATGTTAAAACTAAACAAGCATCATTTGATACTAAAGATTATGAAGCATGA", "aa": "MRRLFKEVFKSLARNKVTLICLTILIFLTTFLFTLLNDVTTSYSSTINSYEKVSKLHDATVDFDVNPSGVIPKSGYNQIGSDNQSIVDKPVVFESSNNGSDISYSIDLPKDQRNYVGLKNTFNNLNYDNENHFINTYDLLNLLYSSKESISDVDVEILKSNPDFSKIRNFSFSGHDREFKLYVKDNKGNIKPLTKRYNLTKNDDITFLNEVRLKNIASIAYGPRGSLNDQKVVDYLYNPSPLFLNVKTKQASFDTKDYEA", "aa_hexdigest": "47d852ac5449e66d0934ad1bbb318985", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 29732.087300000054, "isoelectric_point": 7.699773597717285 }, "id": "GLJLFKCOHC_791", "locus": "GCA013368035_00781" }, { "type": "cds", "sequence": "contig_1", "start": 448407, "stop": 449087, "strand": "-", "frame": 3, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "db_xrefs": [ "COG:COG0357", "COG:J", "EC:2.1.1.170", "GO:0008168", "GO:0031167", "GO:0046118", "KEGG:K03501", "SO:0001217", "UniRef:UniRef50_D1J8E2", "UniRef:UniRef90_D1J8E2" ], "nt": "ATGCTTACGGAAAAAGAAAGCAATAATTTAATATTGCAAAGTCTACCGCAATGTAGCAAAGAAGTCTTAAAAAAATTATATGAATATTATCAATTAATAGAACAAGAAAATAGCAAATATAATTTAACAGGTTTCTATAACGAAAAGCTTCTAAAAGAAGCAATAATTGAATCAATTTTAATTTTTTTAGACATTGAAAAAAATATTTGTAATCTAACTAACAAATCTGTACTCGACATTGGCTCGGGCGCAGGATTTCCTATCCTTCCATATTTCATATATAAGCAAGATTTTTCTTTAACAATTTATGAACCCCAATCAAAAAGAATAAATTTTTTAAATTTGGTTAAAAATGAGTTGAAATTGGAAAATATTGATATAAAAAAAATTCGTGCTGAAGATTCAAATCAAATAGAATGTTTTGATTTTATTAGTGCAAGAGCGGTGAGCGAATTAAAAAATTTATTAGAAATTTCACACCATCTTTTAAAAATTAATGGAATTGCTTGCTTTTTAAAATCAAATAAATATCAGCAAGAAATAGCTAATTCAAACTTTATAGTTAACAAATTAAATATTAATTTAAAAACAAGAATTTTGCCAATATATTTCAATATTGAAAATGTTTTAATTTTTCACCAAAAATTAATAAAAACAGCCTCAGGATTTCCTAGAAAATGA", "aa": "MLTEKESNNLILQSLPQCSKEVLKKLYEYYQLIEQENSKYNLTGFYNEKLLKEAIIESILIFLDIEKNICNLTNKSVLDIGSGAGFPILPYFIYKQDFSLTIYEPQSKRINFLNLVKNELKLENIDIKKIRAEDSNQIECFDFISARAVSELKNLLEISHHLLKINGIACFLKSNKYQQEIANSNFIVNKLNINLKTRILPIYFNIENVLIFHQKLIKTASGFPRK", "aa_hexdigest": "89832b18950ac3a291443d3f437b9095", "start_type": "ATG", "rbs_motif": "AGxAG", "psc": { "uniref90_id": "UniRef90_D1J8E2", "query_cov": 1.0, "subject_cov": 0.9495798319327731, "identity": 0.996, "score": 437.0, "evalue": 1.3e-154, "valid": true, "gene": "rsmG", "product": "16S rRNA (guanine(527)-N(7))-methyltransferase RsmG", "ec_ids": [ "2.1.1.170" ], "uniref50_id": "UniRef50_D1J8E2", "cog_id": "COG0357", "cog_category": "J", "kegg_orthology_id": "K03501", "go_ids": [ "GO:0008168", "GO:0031167", "GO:0046118" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8E2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8E2" ], "product": "Ribosomal RNA small subunit methyltransferase G" }, "genes": [ "rsmG" ], "id": "GLJLFKCOHC_792", "locus": "GCA013368035_00782" }, { "type": "cds", "sequence": "contig_1", "start": 449062, "stop": 450036, "strand": "-", "frame": 2, "gene": "prsA", "product": "Ribose-phosphate pyrophosphokinase", "db_xrefs": [ "COG:COG0462", "COG:F", "EC:2.7.6.1", "KEGG:K00948", "RefSeq:WP_012855629.1", "SO:0001217", "UniParc:UPI0001BC237C", "UniRef:UniRef100_D1J8E3", "UniRef:UniRef50_Q98R83", "UniRef:UniRef90_D1J8E3" ], "nt": "ATGAAAGCAAAAAAAGAAGATAATGTTGTAATTTTTGGCATGGAAAATTCCCAAAAATTAGCACAAGAAATTGGAAAGTATTTAAATAAAGAAATATTGCCAGTTAAAACTACAGTATTTGCAGATGGAGAATGCCTTTTAAAATCAGAAACAACCGTTAGAAATAAGGATGTTTACATTATTTGCAATACCTCAAAACCAGTAAACGATAACATAATGTCATTGCTAATTTTAATCGATTCATTAAGACGTTCATCAGCAAAGAGCATTAATTGCGTTATGACCTACTATGGATATGCAAGACAAGATAGAAAAGCAAGTGGGAGAGATCCAATATCAGCTGCACTTGTTGCTAAGATGTATGAAACAGCGGGTGCTGATAAAATAATTACTATTGACTTGCACAACGCTTCAATTCAAGGATTTTTCAACATTCCTGTTGATGATTTAAGGGGACAATTTATTTTTTCAAATGAATTGCTAAACAGAAAAGAAAAACTAGTAATTGTATCACCAGACCACGGGGGTGCAGTTAGAGCAAGACAATTGTCAGAATTACTAAACAATGAAGAACAAATTGCAATTATTGACAAGCGTCGTACAGGACCTAATAAATGCGAAATTATGGGTGTTTTAGGTGATGTTAAAGATAAAAATGTTTTAATTTATGATGATATTATTGACACAGGTGGAACAATTATTCATGCAGCAGAAGCGTTAAAAGCAAACGGTGCAAAAAATATTATAATTGCAGCTACTCATGGTTTATTTTCAAAAGGGTTTGAAATGTTTGAAAATAACGCAATGATTGATGAAGTTATTATTACTAATTCAATAGATCACAGCCACTTATCAAACTTTAAAAAATTAAGAGAATTATCTTTGGCTCAATTTTTAAGTATGGTAATCAATGCAAACATTGAAAGCAAATCTATAACAGAAGTATATAATGCTTACGGAAAAAGAAAGCAATAA", "aa": "MKAKKEDNVVIFGMENSQKLAQEIGKYLNKEILPVKTTVFADGECLLKSETTVRNKDVYIICNTSKPVNDNIMSLLILIDSLRRSSAKSINCVMTYYGYARQDRKASGRDPISAALVAKMYETAGADKIITIDLHNASIQGFFNIPVDDLRGQFIFSNELLNRKEKLVIVSPDHGGAVRARQLSELLNNEEQIAIIDKRRTGPNKCEIMGVLGDVKDKNVLIYDDIIDTGGTIIHAAEALKANGAKNIIIAATHGLFSKGFEMFENNAMIDEVIITNSIDHSHLSNFKKLRELSLAQFLSMVINANIESKSITEVYNAYGKRKQ", "aa_hexdigest": "4ad11e3f20055d1b39cae4c28e53011b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0001BC237C", "ncbi_nrp_id": "WP_012855629.1", "uniref100_id": "UniRef100_D1J8E3", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC237C", "RefSeq:WP_012855629.1", "UniRef:UniRef100_D1J8E3" ] }, "ips": { "uniref100_id": "UniRef100_D1J8E3", "uniref90_id": "UniRef90_D1J8E3" }, "psc": { "uniref90_id": "UniRef90_D1J8E3", "gene": "PrsA", "product": "Ribose-phosphate pyrophosphokinase", "ec_ids": [ "2.7.6.1" ], "uniref50_id": "UniRef50_Q98R83", "cog_id": "COG0462", "cog_category": "F", "kegg_orthology_id": "K00948" }, "pscc": { "uniref50_id": "UniRef50_Q98R83", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R83" ], "product": "Ribose-phosphate pyrophosphokinase" }, "genes": [ "prsA" ], "id": "GLJLFKCOHC_793", "locus": "GCA013368035_00783" }, { "type": "cds", "sequence": "contig_1", "start": 450230, "stop": 450418, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATACTCAAACCCTTGTAATTCACTTTTTGCGTACTGATAAAACACCATTTATTAAATCAAATGCAACGACCTCGTTATTTATTTCAACATTAATCGGGTGTGCAATAATTAATGCTGTGCCATATATACCTGGAATTAATAAAGGTTTAAGTCTAATGCCTTTGCCAGGAATTTATTATGCTTGA", "aa": "MYTQTLVIHFLRTDKTPFIKSNATTSLFISTLIGCAIINAVPYIPGINKGLSLMPLPGIYYA", "aa_hexdigest": "5bb3a49d8d55cdb133e821f609318a28", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6804.0436, "isoelectric_point": 9.360567665100099 }, "id": "GLJLFKCOHC_794", "locus": "GCA013368035_00784" }, { "type": "cds", "sequence": "contig_1", "start": 450701, "stop": 452005, "strand": "-", "frame": 1, "gene": null, "product": "Magnesium-transporting ATPase, P-type 1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "nt": "TTGTTTGGTATATCAATTGCTGTTGGTTTAACACCAGAAATGCTTCCAATGATAGTGACAGCAAGTTTATCTAAAGGTGCAATTTCACTAGGCAAGGCCAAAACAATAATTAAGAATATTAATTCGATTCAAAATTTCGGATCAATGGACATTTTATGTACAGATAAAACTGGGACATTAACACAAGATGAAGTAATTATGGAACGTCATATGGATGTTACCGGCAAAGAAGATTTTCATGTTTTAAAATATGCTTTTATTAATTCATATTATCAAACAGGATTAAAAAATTTACTTGATAAATCAATAATTAATAAGACTTTTGAAATGGCATCAAAAAACAAAGAACTTGAGGATTTTCAAAGATTTTATGAAAAAATTGACGAAATTCCTTTTGATTTCAAAAGAAAAAGAATGTCTGTTTTAGTTAAATCATTAAAACAAAATAAAGTAACCCTAATTACCAAGGGTGCTGTCGAAGAAATTGTGAGTGTATGTAAAAATATTTACATTGATGGAGAGGTAAAACCTCTTGATAAAAAAACAATAGATAAGGTTCTAAGAAGAGTTGAAGAATTCAATTCAGAAGGACTAAGAGTTATTGCAATCGCTTCTAAAAAATCAACAATTAATGCAGTTGGAAAAATATCGGTAAATGATGAATGCGATATGACATTGATTGGATATTTAACATTTCTTGATCCTCCTAAAGATTCTGCTGCTGCTGCAATTGAAAAATTGCATGAATATGGAGTTGATGTAAAAATATTAACGGGAGATAGTGCGCTTGTAACAAAAGCAGTATGTGCCCAAGTAGGAATAAAAAATAATAGAATTTTACTAGGCAAAGAAGTTGAAGAATTAAGCGATGCTGAACTTAAAATTGAAGTTGAAAAAGTTCAAATTTTTGCAAAATTAAGCCCTGACCAAAAGGCAAGAATTATTTCAATATTGCGTGATAATGGTCATGTTGTTGGCTATATGGGTGACGGAATTAATGATGCACCCGCTATGAAATCAGCCGATGTTTCAATATCAGTTGATACTGCAGTTGATATTGCAAAAGAATCAGCTAACATAATTCTTTTAGAAAAAGACCTAAACGTTTTGGCTAAAGGAATTGTTGAAGGTAGAAAAACACATGCCAATATTAACAAATACATAAAAATGACTGCATCTTCAAACTTTGGAAATATATTTAGTATTTTAATTGCTTCATTTTTAATTCCTTTTGTTCCATTAAACCCTGTACAAATAATATTATTGAATTTGGTATATGATGTTTGCTGTGCAACAATGCCATGA", "aa": "MFGISIAVGLTPEMLPMIVTASLSKGAISLGKAKTIIKNINSIQNFGSMDILCTDKTGTLTQDEVIMERHMDVTGKEDFHVLKYAFINSYYQTGLKNLLDKSIINKTFEMASKNKELEDFQRFYEKIDEIPFDFKRKRMSVLVKSLKQNKVTLITKGAVEEIVSVCKNIYIDGEVKPLDKKTIDKVLRRVEEFNSEGLRVIAIASKKSTINAVGKISVNDECDMTLIGYLTFLDPPKDSAAAAIEKLHEYGVDVKILTGDSALVTKAVCAQVGIKNNRILLGKEVEELSDAELKIEVEKVQIFAKLSPDQKARIISILRDNGHVVGYMGDGINDAPAMKSADVSISVDTAVDIAKESANIILLEKDLNVLAKGIVEGRKTHANINKYIKMTASSNFGNIFSILIASFLIPFVPLNPVQIILLNLVYDVCCATMP", "aa_hexdigest": "6dbfabd812017564fb8b11baea567c9d", "start_type": "TTG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0001C17514", "query_cov": 0.9976958525345622, "subject_cov": 0.9039665970772442, "identity": 0.524, "score": 444.0, "evalue": 1.63e-150, "valid": false, "gene": "mgtA", "product": "magnesium-translocating P-type ATPase", "ec_ids": [ "7.2.2.14" ], "uniref50_id": "UniRef50_A0A2X4XS16", "kegg_orthology_id": "K01531", "go_ids": [ "GO:0015444", "GO:0015693", "GO:0016020" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2X4XS16", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2X4XS16" ], "product": "Magnesium-transporting ATPase, P-type 1" }, "genes": [], "id": "GLJLFKCOHC_795", "locus": "GCA013368035_00785" }, { "type": "cds", "sequence": "contig_1", "start": 452018, "stop": 452578, "strand": "-", "frame": 1, "gene": null, "product": "HAD-IC family P-type ATPase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "nt": "ATGATTTTTTTAAGTGGAATTATTCACTTCGTTCAAGAACAACGTGGGGCATCAAGCGCTGATAAATTAGTTGAGACTGTTGAAACCACTTCGCTTGTTCAAAGGAATGGTATTATGAAGGAAATTCCAATGGATGAGATTGTAGTAGGCGATATTGTGTATTTAGCAGCTGGAGATATTATTCCGGCGGATGTAAGAATATTAGAAGCAAAAGACTTATTTATTTCTCAATCCTCATTAACCGGAGAAAGCGATGCTATTGAAAAATATAAGAACATCGACAGTTCTAAAGAATTTGAAGCAATAACTGATCGTAGCAATTTAGCTTTTATGGGAACCAATGTTATTTCAGGTTCTGCTAAAACTATTGTAATTGCAACCGGCAATGATACAATTATTGGACAAGTTGCTTCTAAAATCAATGAAAAACCAATACCAACCAACTTTGATAAAGGAATTAAAAAAATTTCAATAATGTTCACTATTATAATGGCTGTTATTGTTCCTATAGTATTTTTAATTTTAGGAATTAAAGGTGTTATAGGTGGAGAAAATCAATGA", "aa": "MIFLSGIIHFVQEQRGASSADKLVETVETTSLVQRNGIMKEIPMDEIVVGDIVYLAAGDIIPADVRILEAKDLFISQSSLTGESDAIEKYKNIDSSKEFEAITDRSNLAFMGTNVISGSAKTIVIATGNDTIIGQVASKINEKPIPTNFDKGIKKISIMFTIIMAVIVPIVFLILGIKGVIGGENQ", "aa_hexdigest": "b40783dae5825f36903cdab5183ba662", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002AC587A1", "query_cov": 0.9247311827956989, "subject_cov": 0.900523560209424, "identity": 0.535, "score": 157.0, "evalue": 2.1e-45, "valid": false, "product": "HAD-IC family P-type ATPase", "uniref50_id": "UniRef50_UPI002AC587A1" }, "pscc": { "uniref50_id": "UniRef50_UPI002AC587A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002AC587A1" ], "product": "HAD-IC family P-type ATPase" }, "genes": [], "id": "GLJLFKCOHC_796", "locus": "GCA013368035_00786" }, { "type": "cds", "sequence": "contig_1", "start": 452589, "stop": 452810, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGACAGTCATTCCATCAATTCATGTTATCTTTTTGCCTTTTGATGCAGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCAGCTTCAATTCTTTCTTCATCTTGA", "aa": "MMTVIPSIHVIFLPFDAGNIKSIIIETIANANKMMLKKLKNDLNKRFQTLSAPFFVILFKPYSSLAASILSSS", "aa_hexdigest": "5fd2be3efd989f8b301e8dd44131c75e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8130.694099999997, "isoelectric_point": 9.998095512390137 }, "id": "GLJLFKCOHC_797", "locus": "GCA013368035_00787" }, { "type": "cds", "sequence": "contig_1", "start": 452729, "stop": 452956, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGATAGTTAATATAACAAAAAATAAACTTAAAAATTCTTCAAGAAAAGAAAAACAAATTAAGCAAATCAATACAAGGTTAGTTGAAGCTTCTAATTGTAGTCAAGAAGAATTATTTTTAAAATATCAAACCTCTCAAAACGGATTTCAAGATGAAGAAAGAATTGAAGCTGCAAGAGAAGAATATGGTTTAAATAAAATAACAAAAAAAGGCGCTGATAATGTTTGA", "aa": "MIVNITKNKLKNSSRKEKQIKQINTRLVEASNCSQEELFLKYQTSQNGFQDEERIEAAREEYGLNKITKKGADNV", "aa_hexdigest": "d642ef878636ebb810d5a26cc4121806", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 40, "aa_cov": 0.52, "hmm_cov": 0.975, "evalue": 7.105836122314287e-06, "score": 21.067995071411133, "start": 32, "stop": 70 } ], "seq_stats": { "molecular_weight": 8690.704999999993, "isoelectric_point": 9.130415534973146 }, "id": "GLJLFKCOHC_798", "locus": "GCA013368035_00788" }, { "type": "cds", "sequence": "contig_1", "start": 453147, "stop": 453422, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTGGTTGATACTTATTTTATTACAAAAAGTTGACATTATTTTAGCAGACGAACCTACCAATAGTCTTGATATAGCAAACGCAAGAGATGTTTTAGAATTATTGTCTTCAATATCACAAGAATACAAAAAAACAATTTTAGTAAATATTCACGACATTTCATTAATAAAAACTTATTTTAAGAAATACATTTATGTCAAAGATGGACAGATTATTGAAATTGGTGAAACAAAAAATTTAAATGAATTAAAAATAAAAGATTTATATAATTTTTAA", "aa": "MWLILILLQKVDIILADEPTNSLDIANARDVLELLSSISQEYKKTILVNIHDISLIKTYFKKYIYVKDGQIIEIGETKNLNELKIKDLYNF", "aa_hexdigest": "477e37b7292077b0aedcc44a98fda922", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10612.329799999989, "isoelectric_point": 5.278318977355956 }, "id": "GLJLFKCOHC_799", "locus": "GCA013368035_00789" }, { "type": "cds", "sequence": "contig_1", "start": 453660, "stop": 453998, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00005.33" ], "nt": "ATGAGGTCGATACAAGCCTCACTGAGAAACATTGAGCAGAACACGCTTAATGTTTTTATTTTTGCAATATTTTGTTTTGTATTAATACATTCCAAATATAATTTAAATATGAAAAAAATTGAATTCATCAATGTTGATGCAAGTTATTATAAAAAAAGTCCAATAGTTTTAAAGAACATAAATTTTTCTATTGAAGAGGGTGAATTTATAGCAATAATAGGAAAATCCGGCGCTGGAAAATCAACAATTTTCAATGCTTTATTGAGGCAATTGTTTGTAAAAAAAGGCAATGTAAAAATTAATGATATTGATATTTATAGTGCTTCAAAAAAACAATGA", "aa": "MRSIQASLRNIEQNTLNVFIFAIFCFVLIHSKYNLNMKKIEFINVDASYYKKSPIVLKNINFSIEEGEFIAIIGKSGAGKSTIFNALLRQLFVKKGNVKINDIDIYSASKKQ", "aa_hexdigest": "2cad566fdff7d8cd83c0cec0aa8753fc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00005.33", "name": "ABC_tran", "length": 51, "aa_cov": 0.45535714285714285, "hmm_cov": 1.0, "evalue": 1.153982136883416e-14, "score": 50.55385208129883, "start": 57, "stop": 107 } ], "seq_stats": { "molecular_weight": 12767.844899999986, "isoelectric_point": 9.751826286315914 }, "id": "GLJLFKCOHC_800", "locus": "GCA013368035_00790" }, { "type": "cds", "sequence": "contig_1", "start": 454005, "stop": 454331, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02866.23" ], "nt": "ATGGTTAAAAAAGAAGCATTTGAAATTATTGCTGGTAAAGGCATTACCAATTATGGTATTGGCGAAAACCTTGCCGAAATTACGCAAGCAATTTTGTATGATAGACAAAGAATTTATTCTCTTGGAGTTCAATTACCAAAGGAATATAAACATACCGGAGTTTACTTTGGTTTACCAGTAATCTTGGGTAAGAATGGTTATACACACTTGCCAAAAATTAGACTTGACGAAGATGAACAACAACAATTTGATGAATATTCAAAAGAAATTAAAGAAACAGCAATTCAAGTATTAAAAAATTTGGATGTTGAAATTACTATTAAATAA", "aa": "MVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHTGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK", "aa_hexdigest": "e923af72b38696b56b30a24210c5df95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02866.23", "name": "Ldh_1_C", "length": 97, "aa_cov": 0.8611111111111112, "hmm_cov": 1.0, "evalue": 3.3029641958175115e-15, "score": 51.911155700683594, "start": 2, "stop": 94 } ], "seq_stats": { "molecular_weight": 12373.06749999999, "isoelectric_point": 5.687104988098144 }, "id": "GLJLFKCOHC_801", "locus": "GCA013368035_00791" }, { "type": "cds", "sequence": "contig_1", "start": 454380, "stop": 454973, "strand": "-", "frame": 3, "gene": "mdh", "product": "L-lactate dehydrogenase", "db_xrefs": [ "COG:C", "COG:COG0039", "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4", "UniRef:UniRef90_D1J8E6" ], "nt": "ATGACTAAAATAGGATTAATTGGCGTTGGTGCTGTTGGTAGTTCATATTTATATGCTGCATTAAATAAAAATATAGAAGCAGATTATGTTTTAATTGATAGTTTTCCACAAGTCGCAATTGCGCAAGCAAAAGATTTAAATGATGCTGCTTGTTCAATGGCCAACAATGGTTCAACATTCAAAGCTGGAGATTATTCAGATTTGAAAGATGCTGATATTGTTATTATAACTGCATCAATTAGACCAAAAGAAGGCGTTTTAAAAGATAGAATGGAATTATTGACCGATAATGCAAAATTATTGAGAAAAATCGGCCAAAGCATAAAAGATTCAGGTTTTGATGGTGTTACAATCATTGCCTCAAACCCTGTTGACATTATGGCAACAATTTATCAACAAGTAACCAAGTTTTCTCCAAATAAAGTTATAAGTTCAGGAACTATTCTAGAAACTGCCAGAATGAAAAAATTCCTTTCAGAAAAAATTGGAATTAAAGCACACTCAATTACTGGATTTGTTATTGGTGAACATGGAGCAAGATGTTTAATTCCATTTTCAAAAATAAGAATTGGTTTGGGTTCATTGAAAGATTGA", "aa": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFDGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKD", "aa_hexdigest": "7a5bdf2457aff4b0cd56b60bf7ee2e79", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [ "mdh" ], "truncated": "5-prime", "pseudogene": { "start": 454077, "stop": 454973, "observations": { "mutation": [ 454352, 454382 ], "stop": [ 454352, 454382 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E6", "query_cov": 1.0, "subject_cov": 0.6118012422360248, "identity": 1.0, "score": 380.0, "evalue": 1.37e-131, "gene_start": 1, "gene_end": 197, "reference_start": 1, "reference_end": 197, "reference_sequence": "MTKIGLIGVGAVGSSYLYAALNKNIEADYVLIDSFPQVAIAQAKDLNDAACSMANNGSTFKAGDYSDLKDADIVIITASIRPKEGVLKDRMELLTDNAKLLRKIGQSIKDSGFDGVTIIASNPVDIMATIYQQVTKFSPNKVISSGTILETARMKKFLSEKIGIKAHSITGFVIGEHGARCLIPFSKIRIGLGSLKDWLANGTITNEWLSQLNEMVKKEAFEIIAGKGITNYGIGENLAEITQAILYDRQRIYSLGVQLPKEYKHAGVYFGLPVILGKNGYTHLPKIRLDEDEQQQFDEYSKEIKETAIQVLKNLDVEITIK" }, "paralog": false, "identity": 0.9899328859060402, "score": 572.0, "evalue": 1.12e-209, "description": "Internal stop codon at 454352, 454382. Nonsense mutation around 454352, 454382.", "psc": { "uniref90_id": "UniRef90_D1J8E6", "gene": "Mdh", "product": "L-lactate dehydrogenase", "uniref50_id": "UniRef50_A0A2Z4LNL4", "cog_id": "COG0039", "cog_category": "C", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E6", "UniRef:UniRef50_A0A2Z4LNL4", "COG:COG0039", "COG:C" ] }, "pscc": { "uniref50_id": "UniRef50_A0A2Z4LNL4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2Z4LNL4" ], "product": "L-lactate dehydrogenase" } }, "id": "GLJLFKCOHC_802", "locus": "GCA013368035_00792" }, { "type": "cds", "sequence": "contig_1", "start": 455111, "stop": 456286, "strand": "-", "frame": 1, "gene": "pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "db_xrefs": [ "COG:COG0166", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8E7", "UniRef:UniRef90_D1J8E7" ], "nt": "ATGGAAAATAAGCAAATTATTGACATAAATTTAAAAAAAGCAATTAAAGAAAGTGCACCAAGCCGTTATTATGAGACGGTTCAAAAAATTTTGTTAGATATAAAAAACTCAAAGGTTAAAAATTCAAATTATTTAGGTGTATTCAATGTTTTTAAAAATGTAAAGAAATCTGATTATGAAAAAATTCAAGAAATATCAAGTGAATTATTGTCGAACGGTATTGAATATTTAGTAATAATAGCAACTCAAACAATAATTAATCAAATTAAGGCGCTTGCTGATCTACACTTTGATTTAAGAAAAGAAAATGAATCAATTAAATTGGTTTATGTTAATGAAAATTTTAATAGTTTAGAAGTTGCCAAATTGCTTATTAAACTTCAAAATAAAAAGTTTGCTATTAATGTAATCTCGCAAACTGGCGAATGCTTAGAAACCTTAATAATGTTTAGAGAATTAAAAAACTTATTATATAAAGAGTTAGGCAAGGCGGGAGCTTCTAAATATATCTATGTAACTACAAACAATAATTATGGAAGATTATTTAATGATGCCAAGATAAATCAATATACTCATTTAACAATTTTAGATAATACAGTCGAAAGATTCTTAAGCTATTCACCTGCAATTTTATTGCCAATTGCTTGTATAGGAATTAATATTGAAAAATATTTATCTGGTGCTCATAAGGCTAATGAATTTTTTTTAAATGCACCATTAGAAAAAAACTCAGCAGTTTTATATGCAATCACTAGAAAAATCCTTCATGATAATAATTTTATATTTGAAAATGTTTGCTATTTCGAACAAAATCAAGAATCATTAGCAAAATTGTGGCAAATGCTTTTCAGTGAGACCATTTCATCTATGAACAATAGGATTTATATTGAAACTTGCTTAGGGGGCGTTGATAATAAAACTTTGGCCTCAAATTACACCAACACTCCATTTAAGTGCTTTGATACTTGTTTAAAATTTCAAGAACCTATTATGGACTGCAAAATTTCACAAAATAACGAAAATGAGACGGATGGTTTGAATTATTTAAGCAATTTAACTTATAATAATTTATGTAATCTTGTAATGGAAACATTTATTGAAAACCATGTTAATGTATATAAAATACCTAACATTCAAATCATGATTGACAATTATAACGATAGTACATTGGGTTGA", "aa": "MENKQIIDINLKKAIKESAPSRYYETVQKILLDIKNSKVKNSNYLGVFNVFKNVKKSDYEKIQEISSELLSNGIEYLVIIATQTIINQIKALADLHFDLRKENESIKLVYVNENFNSLEVAKLLIKLQNKKFAINVISQTGECLETLIMFRELKNLLYKELGKAGASKYIYVTTNNNYGRLFNDAKINQYTHLTILDNTVERFLSYSPAILLPIACIGINIEKYLSGAHKANEFFLNAPLEKNSAVLYAITRKILHDNNFIFENVCYFEQNQESLAKLWQMLFSETISSMNNRIYIETCLGGVDNKTLASNYTNTPFKCFDTCLKFQEPIMDCKISQNNENETDGLNYLSNLTYNNLCNLVMETFIENHVNVYKIPNIQIMIDNYNDSTLG", "aa_hexdigest": "05f761136d066ea505d429a41ea31e8a", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J8E7", "query_cov": 1.0, "subject_cov": 0.8866213151927438, "identity": 1.0, "score": 769.0, "evalue": 3.96e-280, "valid": true, "gene": "Pgi", "product": "Glucose-6-phosphate isomerase (GPI)", "uniref50_id": "UniRef50_D1J8E7", "cog_id": "COG0166", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_D1J8E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8E7" ], "product": "Glucose-6-phosphate isomerase (GPI)" }, "genes": [ "pgi" ], "id": "GLJLFKCOHC_803", "locus": "GCA013368035_00793" }, { "type": "cds", "sequence": "contig_1", "start": 456276, "stop": 456515, "strand": "-", "frame": 3, "gene": "yabR", "product": "30S ribosomal protein S1", "db_xrefs": [ "COG:COG1098", "COG:R", "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6", "UniRef:UniRef90_D1J8E8" ], "nt": "TTGAGTATAAATGAAGTTAGTGATTATTACATTAGTGATTTAAAATTAATGTTTAAAATCAATGATATTAAGGAAGTTCAAGTAATTGAAATAATGCCAAATAATGATTTAGTAGTTTCATTTAAAAGAATTCACCCCAAGGAATTAAGAAACCCTTTTGAATTTAAACTAGATAGTCAAGATGCAGAATTTGATGGATTATTAGAATTTACTAACAAAGGAATTAATTATGGAAAATAA", "aa": "MSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK", "aa_hexdigest": "e2ea816ef770b351b97bd2726dfdb568", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "yabR" ], "truncated": "3-prime", "pseudogene": { "start": 456276, "stop": 456611, "observations": { "mutation": [ 456563 ], "stop": [ 456563 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8E8", "query_cov": 1.0, "subject_cov": 0.7117117117117117, "identity": 0.987, "score": 159.0, "evalue": 2.4e-49, "gene_start": 1, "gene_end": 79, "reference_start": 33, "reference_end": 111, "reference_sequence": "MNYEIGDIFKAKIIKVWKNNIILQTKEGCKCFLSINEVSDYYISDLKLMFKINDIKEVQVIEIMPNNDLVVSFKRIHPKELRNPFEFKLDSQDAEFDGLLEFTNKGINYGK" }, "paralog": false, "identity": 0.990990990990991, "score": 222.0, "evalue": 1.38e-76, "description": "Internal stop codon at 456563. Nonsense mutation around 456563.", "psc": { "uniref90_id": "UniRef90_D1J8E8", "gene": "YabR", "product": "30S ribosomal protein S1", "uniref50_id": "UniRef50_A0A3T0TTP6", "cog_id": "COG1098", "cog_category": "R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8E8", "UniRef:UniRef50_A0A3T0TTP6", "COG:COG1098", "COG:R" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3T0TTP6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TTP6" ], "product": "S1 motif domain-containing protein" } }, "id": "GLJLFKCOHC_804", "locus": "GCA013368035_00794" }, { "type": "cds", "sequence": "contig_1", "start": 457098, "stop": 457295, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTTTTATAACGTCAACTTTATTAAAAAATTCATTGCTAAAAAATGATGTTATTCCTTTACTGCAAACAAAAACAAGGGCTTTTTATTTTGACAAATTAGATGAACAATATGCTGATGGTTCTGACAATGATCCACTAAGAAAAATAGCAAAAATAGCAAATGACTTATTTAACAAAAAGCCTTATAAATCATGA", "aa": "MAFITSTLLKNSLLKNDVIPLLQTKTRAFYFDKLDEQYADGSDNDPLRKIAKIANDLFNKKPYKS", "aa_hexdigest": "2cbe7982fbd40095999b4a6c4a0ec61f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7481.536400000003, "isoelectric_point": 9.301321220397949 }, "id": "GLJLFKCOHC_805", "locus": "GCA013368035_00795" }, { "type": "cds", "sequence": "contig_1", "start": 457758, "stop": 457949, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAACAGTAACTGATCCAATAAAATTTAATGTCTTAGTTGTTGATAAATCAATACCAAAATCCGCTTATCTTGCTTTTGCAAATTCAATAATTAATTTAGCAAAAGAAAAAAAAGATACATACGGACCAAATGTTGGTTTCAATGAATATGAACTAATTAATGAATCTGACTATTCAAAATATTTTTAA", "aa": "MLTVTDPIKFNVLVVDKSIPKSAYLAFANSIINLAKEKKDTYGPNVGFNEYELINESDYSKYF", "aa_hexdigest": "89008491af5116d5a62bd31b6a81041a", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7168.088800000001, "isoelectric_point": 5.135198402404785 }, "id": "GLJLFKCOHC_806", "locus": "GCA013368035_00796" }, { "type": "ncRNA-region", "class": [ "riboswitch", "SO:0000035" ], "sequence": "contig_1", "start": 457983, "stop": 458070, "strand": "+", "label": "TPP", "product": "TPP riboswitch aptamer (THI element)", "score": 57.5, "evalue": 3.8e-07, "db_xrefs": [ "RFAM:RF00059", "SO:0000035" ], "nt": "TATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGC", "id": "GLJLFKCOHC_44" }, { "type": "cds", "sequence": "contig_1", "start": 458095, "stop": 458244, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAATTTAAAAAATATTTGCTTATAGGGGCAGCGGTATTGCCTACAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGA", "aa": "MLKFKKYLLIGAAVLPTMVGASVLSSSCVDKISNNTKTYENLKFSINKP", "aa_hexdigest": "3f9db41499f69597855c27960265989f", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5364.346699999999, "isoelectric_point": 9.732872581481931 }, "id": "GLJLFKCOHC_807", "locus": "GCA013368035_00797" }, { "type": "cds", "sequence": "contig_1", "start": 458416, "stop": 458595, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTACAATCTAGAAAAAGATAAATCGAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGTCTAGATGATGATCCACTAAGAAAAATAGCTCAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGA", "aa": "MYNLEKDKSNIHPFLQTMTRAFVFDQDFNAQYSNGLDDDPLRKIAQSAFDLFNKKPFEN", "aa_hexdigest": "29056b1ccd79ce8e4a91692201dfe7df", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6975.697100000001, "isoelectric_point": 5.030216789245605 }, "id": "GLJLFKCOHC_808", "locus": "GCA013368035_00798" }, { "type": "cds", "sequence": "contig_1", "start": 458773, "stop": 459147, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGGAAGTAGTTCTAGTGGTTCAAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAACGAAACAAAATACATCAAAGGTAAGATTAAAAATATTGGAAAGGGTGCATTTTCTAAATACCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACACATAGTAAAGGCAGCGCAATCTATAAATTGGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACCGAACCTTTAAAATATAACATTTTTGCCACATCATCAAATATTCCAAAAGAATTAAGGGAAATAATTGCAAATGCAATGGTCGAAGTTTGA", "aa": "MHGSSSSGSKYLLQEALFKKHFNLKGNEFKSFLEDKQGNETKYIKGKIKNIGKGAFSKYHIVFDDLGSFAYTHSKGSAIYKLDDKNSKIEFLTVTEPLKYNIFATSSNIPKELREIIANAMVEV", "aa_hexdigest": "22ef8de0fcb32d9041c9eca68edac914", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14004.834999999983, "isoelectric_point": 9.30170803070068 }, "id": "GLJLFKCOHC_809", "locus": "GCA013368035_00799" }, { "type": "cds", "sequence": "contig_1", "start": 459984, "stop": 460295, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATCTACCCAAATTCAATCAATGTCAATTACTTTCATGAACTTCTAATTCCACTACTAATATTGGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTGTTGTTTTATTCAAGGACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAGCAATTGCAATAGGCTTAATAATAACTATCTTTGCATGTTTATCATTAAGGGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAAGAGTTATTTAATGGTGAATGA", "aa": "MIYPNSINVNYFHELLIPLLILATFLIFIEFCNFIISNLLFYSRTQKVNIKKYAKNSTIKKIIIFIAIAIGLIITIFACLSLRGEKFYSTQGNYYFKELFNGE", "aa_hexdigest": "287df4b89f3c78c1228a91d746767a75", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12032.218199999985, "isoelectric_point": 9.329364967346194 }, "id": "GLJLFKCOHC_810", "locus": "GCA013368035_00800" }, { "type": "cds", "sequence": "contig_1", "start": 460527, "stop": 460853, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTGGAGAGCTCTATTAAAAAAATCTCGACAAACAAGATAAATGAACTAAAAATCAAAGGATATTCAAAATTTTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATATGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCTGATATTGCACCATATCTATGA", "aa": "MFNKLEAAFVIAFAIHSSSTISRMLESSIKKISTNKINELKIKGYSKFYIFRNFIMPTIKLDLITFMTFEIEKITRNFITYGLFSASLLGSETNLGRYKEISDIAPYL", "aa_hexdigest": "47327d05e1ae3056344029c963150f8f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12436.487199999983, "isoelectric_point": 9.55900135040283 }, "id": "GLJLFKCOHC_811", "locus": "GCA013368035_00801" }, { "type": "tRNA", "sequence": "contig_1", "start": 461073, "stop": 461146, "strand": "+", "gene": "trnW", "product": "tRNA-Trp(cca)", "amino_acid": "Trp", "anti_codon": "cca", "score": 64.3, "nt": "AGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCA", "db_xrefs": [ "SO:0000271" ], "anti_codon_pos": [ 461105, 461107 ], "id": "GLJLFKCOHC_22", "locus": "GCA013368035_00802" }, { "type": "cds", "sequence": "contig_1", "start": 461290, "stop": 461910, "strand": "-", "frame": 2, "gene": null, "product": "Methionine adenosyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377WSC8" ], "nt": "ATGAAAAGCCAAGTGACATTGGAATATGATGATGAAAGCAATAAAATAACTGTTAAAAAAGTTTTAATGTCAATTCAACATGCTGAAAATGCAAATTTAAAAGAATTTAAAGATTTTATAAAGAAAAATATTGTTGATTATGTGTTAAAAGAATTTAATTTCAATTTGGATTATGATTTATTGATTAATCCAACTGGTAGATTTGTAATTGGTGGCCCTGTTGGAGATACTGGGCTTACAGGAAGAAAAATAATTGTTGATACATACGGCGGTTTTGCTCACCATGGTGGAGGAGCATTTTCAGGGAAAGATGCTACAAAAATAGATAGGTCTGCTGCTTATATGGCAAGGTATATTGCTAAAAACTTAGTTGCTGCTAAGATAGCAGACAAGATTGAAATTCAATTGTCTTATGCAATAGGCTTGCCACGCCCTCAATCTATTTATATAAATTCATTTAATTCATCACAATATAATGATGAACAAATTGCTGACATTATTGAACATGTATTTGATTTAAGCGTTGAACAAATTATAAAAAAATTAGATTTATTAAAGCCAATATATCTACAAACTGCAACATATGGACATTTTGGACGAAACGACCTTAATTTAAGTTGA", "aa": "MKSQVTLEYDDESNKITVKKVLMSIQHAENANLKEFKDFIKKNIVDYVLKEFNFNLDYDLLINPTGRFVIGGPVGDTGLTGRKIIVDTYGGFAHHGGGAFSGKDATKIDRSAAYMARYIAKNLVAAKIADKIEIQLSYAIGLPRPQSIYINSFNSSQYNDEQIADIIEHVFDLSVEQIIKKLDLLKPIYLQTATYGHFGRNDLNLS", "aa_hexdigest": "c710413c409e911dbd567aebf832970c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0010E99B0D", "query_cov": 0.9902912621359223, "subject_cov": 0.9577464788732394, "identity": 0.5539999999999999, "score": 233.0, "evalue": 8.48e-75, "valid": false, "gene": "metK", "product": "methionine adenosyltransferase", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_A0A377WSC8", "kegg_orthology_id": "K00789", "go_ids": [ "GO:0004478", "GO:0005524", "GO:0006556" ] }, "pscc": { "uniref50_id": "UniRef50_A0A377WSC8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A377WSC8" ], "product": "Methionine adenosyltransferase" }, "genes": [], "id": "GLJLFKCOHC_812", "locus": "GCA013368035_00803" }, { "type": "cds", "sequence": "contig_1", "start": 461992, "stop": 462165, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02772.21" ], "nt": "TTGAAACCATTAGGATATGATGAAAATGATTTTAATATAATTAATGGTATTGCTCCACAAAGTGCCGATATTGCTTTGGGTGTCAATAAAGAAGAAGACCATGAATTAGGTGCTGGTGATCAAGGGATTATTTTTGGATATGCAACTAATGAAAATGAATTCTATATGCCTTGA", "aa": "MKPLGYDENDFNIINGIAPQSADIALGVNKEEDHELGAGDQGIIFGYATNENEFYMP", "aa_hexdigest": "3f582acdd074f3643bb14cd6c36d0b7c", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02772.21", "name": "S-AdoMet_synt_M", "length": 22, "aa_cov": 0.38596491228070173, "hmm_cov": 1.0, "evalue": 5.1800996905549244e-09, "score": 31.716350555419922, "start": 36, "stop": 57 } ], "seq_stats": { "molecular_weight": 6247.751999999999, "isoelectric_point": 4.0500284194946286 }, "id": "GLJLFKCOHC_813", "locus": "GCA013368035_00804" }, { "type": "cds", "sequence": "contig_1", "start": 462172, "stop": 462378, "strand": "-", "frame": 2, "gene": null, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "nt": "ATGAAAAAATTATTAACATCAGAAAGTGTAGGGGCAGGTCACCCTGATAAAATATGTGACCAAATTAGTGACGCCATATTAGATAGTTTGTTGACAAATGATCCATATGCAAGAGTCGCTTGCGATGTTTTAGCAAACAACAACATCATTTATATAGGAGGTCAAATAACTACAAAGGCATATTCTGACACAATTAAAGAAGCATGA", "aa": "MKKLLTSESVGAGHPDKICDQISDAILDSLLTNDPYARVACDVLANNNIIYIGGQITTKAYSDTIKEA", "aa_hexdigest": "8e03e4d2757fc48613e17f520dcae434", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00387A9BEE", "query_cov": 1.0, "subject_cov": 0.9444444444444444, "identity": 0.5589999999999999, "score": 81.3, "evalue": 6.23e-19, "valid": false, "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein", "ec_ids": [ "2.5.1.6" ], "uniref50_id": "UniRef50_UPI00387A9BEE", "kegg_orthology_id": "K00789" }, "pscc": { "uniref50_id": "UniRef50_UPI00387A9BEE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00387A9BEE" ], "product": "S-adenosylmethionine synthetase N-terminal domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_814", "locus": "GCA013368035_00805" }, { "type": "cds", "sequence": "contig_1", "start": 462623, "stop": 462886, "strand": "-", "frame": 1, "gene": null, "product": "Aspartate--ammonia ligase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "nt": "ATGTTGAATGGGGATTTAATTGTCTATGATTCAGTAAATAAAAAAGCATTGGAAATTAGCAGTATGGGAATTAGAGTTGATAAAGATTCTTTAATTAAACAATCAAATAAAACCGAAGAAGAAATTAAAAAATTGAGTCCATATCATTGTGATTTAATAGAAGGCAAATTGCCATATACGATTGGTGGTGGCCTAGGCCAAAGTAGAATTGCAATGTTTCTATTAGAAAAACATCATATTGGTGAAGTTCAAGCATCTGCATGA", "aa": "MLNGDLIVYDSVNKKALEISSMGIRVDKDSLIKQSNKTEEEIKKLSPYHCDLIEGKLPYTIGGGLGQSRIAMFLLEKHHIGEVQASA", "aa_hexdigest": "9c327a574bde271a5fce32e86d2e8ad5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A448ZXQ9", "query_cov": 0.9655172413793104, "subject_cov": 0.8, "identity": 0.655, "score": 117.0, "evalue": 1.62e-32, "valid": false, "product": "Aspartate--ammonia ligase", "uniref50_id": "UniRef50_A0A448ZXQ9" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXQ9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXQ9" ], "product": "Aspartate--ammonia ligase" }, "genes": [], "id": "GLJLFKCOHC_815", "locus": "GCA013368035_00806" }, { "type": "cds", "sequence": "contig_1", "start": 462887, "stop": 463081, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "TTGAGTCAAAAGTTAGCTAAAAATATTTATTTTATTAGTTCTAGCCATTTGTATAATCTTTATCCAAACCTAAGTCCCGAAGAACGCGAAGAAGCAATTTGCAAAGAATATGGTTCTGTTTTTATTTATCAAATAGGATACAATTTACCTGATGGAATTCCACATTCAAAAAGAGCATTCGACTATGATGATTGA", "aa": "MSQKLAKNIYFISSSHLYNLYPNLSPEEREEAICKEYGSVFIYQIGYNLPDGIPHSKRAFDYDD", "aa_hexdigest": "1c921a58ad1b4b8aaf168dea11ff5f2d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 63, "aa_cov": 0.984375, "hmm_cov": 1.0, "evalue": 3.313248198371347e-23, "score": 78.02096557617188, "start": 2, "stop": 64 } ], "seq_stats": { "molecular_weight": 7480.2697, "isoelectric_point": 5.167596626281735 }, "id": "GLJLFKCOHC_816", "locus": "GCA013368035_00807" }, { "type": "cds", "sequence": "contig_1", "start": 463322, "stop": 463552, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03590.20" ], "nt": "ATGCCATCATCAAAATTATCATTGAAAGATACCCAATTTGCTATTGAAAAATTAAAAGATCTGTTTTCACATCAACTTCATAGAACATTGAATTTGGTCAGGGTTTCAGCGCCATTGTTTGTAAAAACCAAGAGTGGTTTAAATGACGGTTTGAACGGAGAAAAACCAGTTGTATTTTTTGCAGACGAAGTAGAAGATGAATTGCAAATTATTCATTCACTTGCCAAATGA", "aa": "MPSSKLSLKDTQFAIEKLKDLFSHQLHRTLNLVRVSAPLFVKTKSGLNDGLNGEKPVVFFADEVEDELQIIHSLAK", "aa_hexdigest": "4ebbf96f33764ac1b55f29343995a790", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03590.20", "name": "AsnA", "length": 67, "aa_cov": 0.8552631578947368, "hmm_cov": 1.0, "evalue": 7.791483498108978e-20, "score": 66.99182891845703, "start": 12, "stop": 76 } ], "seq_stats": { "molecular_weight": 8533.764299999999, "isoelectric_point": 6.824282646179199 }, "id": "GLJLFKCOHC_817", "locus": "GCA013368035_00808" }, { "type": "cds", "sequence": "contig_1", "start": 463686, "stop": 464141, "strand": "-", "frame": 3, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGGATGCTGTAAAATTGGCTAACTTGTTGCAATACCAAGGTGCATTAAACGAAGCTCAATTGCGTAACATGCGTTTAGCATTAGACACTGCTTACCATGGTAAAGGTGTAAAAGGACATGAAGACTTGCCAGCAGGTGCTTCAATCAACCAAGTAAGAGAATTCATGAAGAAGAATTCATCACTAGGAATTGGTGACATAACATCAGAATCAAGACGTTACCTATCATATGTTGGTCAAGCAACCTCATATAACTCAGGTAAGGCAGTTATGATGGATCTATATACAAAAGTTCAAAAGAAACTAGGATTAACAAGAAGAGAATTTGTTGAAAAAGATGATCATAAATATGTAAAAGAATTCTTTGATGCTTTATTACGTAATAGTGCATTGCCAATGGATGCTTTAATTAAATCAGTAAGTGCTAAATACGGCCTAACTGTTGAAAAGAAATAG", "aa": "MDAVKLANLLQYQGALNEAQLRNMRLALDTAYHGKGVKGHEDLPAGASINQVREFMKKNSSLGIGDITSESRRYLSYVGQATSYNSGKAVMMDLYTKVQKKLGLTRREFVEKDDHKYVKEFFDALLRNSALPMDALIKSVSAKYGLTVEKK", "aa_hexdigest": "4d50d49ccdfb8a120f6db0ff6aab6e4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.8675496688741722, "subject_cov": 1.0234375, "identity": 0.542, "score": 139.0, "evalue": 1.16e-39, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "GLJLFKCOHC_818", "locus": "GCA013368035_00809" }, { "type": "cds", "sequence": "contig_1", "start": 464193, "stop": 464312, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCAGTTGACTTTTCAACAGCACATGGTATAACAAACTTCTTTACTGCTAAAACAGAAGCAGAAGTTACAGATGACATGATTCAACAAATTAAAGACTTACACAATGGTGTATATTGA", "aa": "MPVDFSTAHGITNFFTAKTEAEVTDDMIQQIKDLHNGVY", "aa_hexdigest": "0fee1761c63433642153f643aeb86683", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4385.838, "isoelectric_point": 4.507241249084472 }, "id": "GLJLFKCOHC_819", "locus": "GCA013368035_00810" }, { "type": "cds", "sequence": "contig_1", "start": 464397, "stop": 464513, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGACACCACAACCAATTAATGTATGCTGAAAGATATCTATCATCAGTTGGAGAATTTGGTAAATACAAATTAGGAAATGTATTCCACTACACTTCATACGTTGAAGGTTGA", "aa": "MMGHHNQLMYAERYLSSVGEFGKYKLGNVFHYTSYVEG", "aa_hexdigest": "1cdf4e419f264170aa99281fde8646ac", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4444.977500000001, "isoelectric_point": 6.812403297424316 }, "id": "GLJLFKCOHC_820", "locus": "GCA013368035_00811" }, { "type": "cds", "sequence": "contig_1", "start": 464544, "stop": 464858, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAAATATGTTGATGAATTAACAAAATTCAATTATAATCAATTACTAAGTGAAAAAAATAAAGCTACAAAACATGGTTCGATAACAAACGAAGAATTCTATTATGGCGGATTGTCGGCATTTAAGGCATATGAACAATTTAAGAAGACTACTCAAGATTATGGACGTAAATTCTTTGCAAATCCTGTCCCTGATTATGATATTCAAACATATGACTTTTCTAGAAGAGAATTTTCAGGTGTTGGAGCATACAACTCAGGCATTCAAAAATTCATGTTTAATGTCGATCCATACTTCTCATTACCAAAATGA", "aa": "MKKYVDELTKFNYNQLLSEKNKATKHGSITNEEFYYGGLSAFKAYEQFKKTTQDYGRKFFANPVPDYDIQTYDFSRREFSGVGAYNSGIQKFMFNVDPYFSLPK", "aa_hexdigest": "52b7a9cb00b435a295ac761c6f14392a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12240.54639999999, "isoelectric_point": 9.001930046081544 }, "id": "GLJLFKCOHC_821", "locus": "GCA013368035_00812" }, { "type": "cds", "sequence": "contig_1", "start": 464971, "stop": 465147, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTCCTTTTTCATCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCATATTTAATTTTGGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAA", "aa": "MFPFSSRITTVSSFISSGPIPSASYLILASHFLPFPTIKSAAWLAIFFEFSADFFVVS", "aa_hexdigest": "ea56eb80e1eb0db2d5e379dfa934a12c", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6382.334099999998, "isoelectric_point": 6.505302238464354 }, "id": "GLJLFKCOHC_822", "locus": "GCA013368035_00813" }, { "type": "cds", "sequence": "contig_1", "start": 465060, "stop": 465707, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGCATTTACAAATGTGCTTGGAAGTAGAAGCTCGCTACCATTTAAACAACTATTAGAAAACTTCAAAGAAGCAGTGCAACATAAAGTTGTTATGTCAAAAGTTTTCTCAAAAATGGTAATCAATGCTTTTGTGGGAAGATTATTTCAAACACAACTAACTAAATTTGTAAATGACAATTCTAAAAATGAAATTTCGGTTGTAGAATTAATTAATTCTTCTAGCTTGCCAACAGACAAAAAAGAATTATTAAAATATTATGCAACTACATATTACAATGCTGCAACTCATGGATTAGGTGAAGACATTAAAGAATTAAAACTTTATAAAGAAAATAAAGTTAATGAAAAAGAATTGTCTATTGATGCAGAAGATGCTAGTGGCAAAATTGTTAAATTATATGGTTTAGGATTAACTGATAAAGACTTAAATCAAAGAAATGTTGGTTTAGGATTTGCTGAAGGAGATGCTACCGTAAATGGTCAATCGATGTATCGTCAAATTCTAAAGATGGCTACAACTTCTGATTTAACAGATGATCAAGTAAATAATATTGGTTATGAAACAACAAAGAAATCTGCTGAAAACTCAAAGAAAATTGCTAACCAAGCAGCTGATTTAATTGTTGGAAAAGGCAAAAAATGA", "aa": "MNAFTNVLGSRSSLPFKQLLENFKEAVQHKVVMSKVFSKMVINAFVGRLFQTQLTKFVNDNSKNEISVVELINSSSLPTDKKELLKYYATTYYNAATHGLGEDIKELKLYKENKVNEKELSIDAEDASGKIVKLYGLGLTDKDLNQRNVGLGFAEGDATVNGQSMYRQILKMATTSDLTDDQVNNIGYETTKKSAENSKKIANQAADLIVGKGKK", "aa_hexdigest": "64b12958486d5e7132b820b3b1c9e82c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 23858.8746, "isoelectric_point": 8.98716678619385 }, "id": "GLJLFKCOHC_823", "locus": "GCA013368035_00814" }, { "type": "cds", "sequence": "contig_1", "start": 465813, "stop": 466925, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACAAACTAGAATTCTAATTTCATTAGGTTCAATTTTTGGCGTAGCAGCCTTAATGCCTATGGCAGCATCATGTACACATACAAATAATGATGAATTAAACAAAAAAATTGAATCTTTAGAAAAGCAATTAAATGAAGCAAAAGCAAATTCTCAAAAAGATCAAGCACAAATTAATGATCTTATGAAGCAATTGCAAGAAACAAAAAATAATTCAAAAACAATTGAAGAAAAAGAAAAATTAGTTAAAAAATTGCAAGAAGATTTAAATAAAATCCAACAAGAAAGAACAGCTAAATATCAAGCAGAATTAAATTCTACAAACAAGGCTATTGTTGAAATTCCTAAGAAATATGAAGAATTAGAACAAAAAAATGAAGATCTTAAAGATCAATTAAGCAGCTTAAAAATGAAGCTAATGACTGCTGAAAGCAAGTTTAAAAGTTCAGGAAGAAAAATAGAAGGCTATTTAAAAACATCAACAGAAAAAGAAAACAAAATTAAAGAATCTAAAACTAAATTAGTTGACCTAAGAAAAACTTTGGAAGAATTAAACAAAAATTTAGAAGAATTAGAAAAACAAAATTCTGATGGCACAAAAAATAATGAAATATCATCAAAAAAAGAAGAAATTAAATCTAAAAATTTAGAAATTAAGCAAGAAGAAGAAAAGTTTGAAGGTCTTCAAACAGAATTAAAAACTCAAAAAGATAAAATCATTGAAGAAACTAATAAAAAGTCAGAACTTGAAATTCAAGTTGCAGATTTAAAAGCAAAAAAAGAAAATATTTCTAAAGAAATTGAAGCTAATCAAAAGGAATTAGAAGCGCTAGACAAAGAATATAATGATAGCGTTAAAAAGAGCGATGAATTAAGCAATTTACTTTCAAAAGAAAATGATAATTCACCAGCTAGCCAAGAAGCAGCCGCAAGACATATTTTAGATTTAAAAGGTGATCTTGAATCTGAACTAAAGAAAGAAAAATTAAATGGAATAATTGCTCCTACTGCTGAACAATCTAAGAAAATTTCTGGAATTTATGGTAAATATATTGAAAAAATTTCAAAAATAAATGACGCTTCACTAACAAGTGATTCTTTAGCATGA", "aa": "MKKQTRILISLGSIFGVAALMPMAASCTHTNNDELNKKIESLEKQLNEAKANSQKDQAQINDLMKQLQETKNNSKTIEEKEKLVKKLQEDLNKIQQERTAKYQAELNSTNKAIVEIPKKYEELEQKNEDLKDQLSSLKMKLMTAESKFKSSGRKIEGYLKTSTEKENKIKESKTKLVDLRKTLEELNKNLEELEKQNSDGTKNNEISSKKEEIKSKNLEIKQEEEKFEGLQTELKTQKDKIIEETNKKSELEIQVADLKAKKENISKEIEANQKELEALDKEYNDSVKKSDELSNLLSKENDNSPASQEAAARHILDLKGDLESELKKEKLNGIIAPTAEQSKKISGIYGKYIEKISKINDASLTSDSLA", "aa_hexdigest": "46cf0ee88021acbdfa7493199f8b1a93", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 42092.19200000005, "isoelectric_point": 6.399752235412598 }, "id": "GLJLFKCOHC_824", "locus": "GCA013368035_00815" }, { "type": "rRNA", "sequence": "contig_1", "start": 467289, "stop": 467393, "strand": "+", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.2e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "GLJLFKCOHC_37", "locus": "GCA013368035_00816" }, { "type": "cds", "sequence": "contig_1", "start": 467602, "stop": 467754, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTCCCCATTTCTATTATTATTTCTATGTATATAAATATGCATTAGGATATATCGTAGCAAATGTATTTTTCCAAAAATACAAACAAGAAGGCCAAGAAGCATTAAAAAACTATATAAATAAATTTTTAAGTGCCGGAGACATCGATTGA", "aa": "MVPHFYYYFYVYKYALGYIVANVFFQKYKQEGQEALKNYINKFLSAGDID", "aa_hexdigest": "ccab9e932d67397b07838c4ee1e3418c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6061.8657, "isoelectric_point": 7.928853797912598 }, "id": "GLJLFKCOHC_825", "locus": "GCA013368035_00817" }, { "type": "cds", "sequence": "contig_1", "start": 467911, "stop": 468309, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCTTATGAATATCCAGAAGTTGTTAAAAAAGCTATTGATGAACGTTGGGTTGATTATATTAATGTCCCATCTAAACGAAGCGGCGCTTATTCTATAGGTGGGGCAGCAGGAATGAAAAAAATTTATATATTGATGAATTTTGATGGAACATTGGAATCAGTCAATACATTATGCCACGAAATGGGACATTCAATGCATTCATATTTTTCTAATAAATGCCAATCTCCACTTAGAAGCTCTTATCCAATATTTTTAGCAGAAATTGCTTCGATATTTAATGAATTATTACTAAATGATTATTTAATAAGCAAATCAAAAAATATTCAACAACAATTTTATTTACTTGATTCAAGTATTAATGATTTTATTGGCACAGTTTTGCGCCAAACAATGTGA", "aa": "MPYEYPEVVKKAIDERWVDYINVPSKRSGAYSIGGAAGMKKIYILMNFDGTLESVNTLCHEMGHSMHSYFSNKCQSPLRSSYPIFLAEIASIFNELLLNDYLISKSKNIQQQFYLLDSSINDFIGTVLRQTM", "aa_hexdigest": "f99638569fbdc57d5d1af3e7a4bf5593", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15091.162899999981, "isoelectric_point": 6.267885780334471 }, "id": "GLJLFKCOHC_826", "locus": "GCA013368035_00818" }, { "type": "cds", "sequence": "contig_1", "start": 468394, "stop": 468876, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAGAACTTGATCATAAATTGTCAGATGATGTTGAAAATTATTTAAATGATACTGCAAGCGGTTCACCTGTACCTGAAGATATTTTTTCAATAATAAGCGATAGTGAAGTTGATTTTGGCAATGTTTTAATTAAAAATAAAAAAATAAAAATAACTGAAGGAAATAGAATAGCTTTATTAAAAAATAAAGATGAACAAGTTAGAAAAGATGTTTATTTTAATTATTTAAATGGATATTTAAAAAACAAACAATCATTAGCGAGATTGCTATATCAACATATTAAAGAAATATCAGTTAATGCTTTATATAGAAAATATAATTCATCTCTTGAATCTATTTTGTTGCAAGATCATGTTGATAAAAAGTTATTGAACATTATTTATGATGCAGTTCAAAAACATATGTATATATTTAAAAAATATAACAATGCCAAAAAACAATTTTTTAAAGCTAAATTCAATAAAAAATTAGAAAAATGA", "aa": "MQELDHKLSDDVENYLNDTASGSPVPEDIFSIISDSEVDFGNVLIKNKKIKITEGNRIALLKNKDEQVRKDVYFNYLNGYLKNKQSLARLLYQHIKEISVNALYRKYNSSLESILLQDHVDKKLLNIIYDAVQKHMYIFKKYNNAKKQFFKAKFNKKLEK", "aa_hexdigest": "8cd7040db18df7e979293f3522fcdff7", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18823.412900000003, "isoelectric_point": 9.33084774017334 }, "id": "GLJLFKCOHC_827", "locus": "GCA013368035_00819" }, { "type": "cds", "sequence": "contig_1", "start": 468925, "stop": 469188, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATTCAAAAATAATTGAAATAAAAGATAGCAAATACAATTCATTAGACCAATATGTTGAATATATTGAACTTTCAAAAAAATTCCTAATTTTAGGCAATAGAATTTCTAATTATTTGGATAATAAATTAAATATAAATATTGTTGATCCCACAATAAACAAAACAATTTCATTATTTGAAACAAAATCCCAAGAATATGCTAAAAAGTTAGGTTCAGAAGCCAACAGAATTTTTCAACATAAAGACAAAATCAAAACATGA", "aa": "MYSKIIEIKDSKYNSLDQYVEYIELSKKFLILGNRISNYLDNKLNINIVDPTINKTISLFETKSQEYAKKLGSEANRIFQHKDKIKT", "aa_hexdigest": "224e375b41de36fb76ea8eb75d953e69", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10248.67349999999, "isoelectric_point": 9.273406410217284 }, "id": "GLJLFKCOHC_828", "locus": "GCA013368035_00820" }, { "type": "cds", "sequence": "contig_1", "start": 469299, "stop": 469814, "strand": "-", "frame": 3, "gene": "apt", "product": "adenine phosphoribosyltransferase", "db_xrefs": [ "COG:COG0503", "COG:F", "EC:2.4.2.7", "GO:0003999", "GO:0009116", "KEGG:K00759", "RefSeq:WP_012855642.1", "SO:0001217", "UniParc:UPI0001BC2388", "UniRef:UniRef100_D1J8F6", "UniRef:UniRef50_Q9RFQ2", "UniRef:UniRef90_D1J8F6" ], "nt": "ATGGATTTAAAAAAGTATATAAGAACTGTTGATGACTTTCCACAAAAAGGTGTCAAATTTAAAGATATTTCGCTTTTATTAGCCAATGGACAAGCGCTACATTATACAATTGATGAAATGGCAAAATTAGCAGAAGGTGCAGATATTATTGTGGGTCCTGATGCAAGAGGATTTTTATTTGGAACACCAGTAGCTGCTAAATTGTCAAAACCATTTATTATGGTAAGAAAAAAAGGCAAATTGCCCGGCGAAGTTATAAGCTGCGATTATGGACTTGAATATGGTAGAAATATTTTAGAAATTCAAAAAGGTTTAGTTAAACCAGGTCAAAAAGCCGTCATAGTTGACGATGTTTTAGCAACCGGGGGTACATTGGAAGCAATTACTAAATTATTAACAGAACAAGGTGTAATTATCAGTAGAATAATCGTTTTAATGGAATTAGATGGCTTTAATTCTCATGAAAGATTGCCATACGATATAAAAAGTTTAATCTATATTAATAAAGGGGAATAA", "aa": "MDLKKYIRTVDDFPQKGVKFKDISLLLANGQALHYTIDEMAKLAEGADIIVGPDARGFLFGTPVAAKLSKPFIMVRKKGKLPGEVISCDYGLEYGRNILEIQKGLVKPGQKAVIVDDVLATGGTLEAITKLLTEQGVIISRIIVLMELDGFNSHERLPYDIKSLIYINKGE", "aa_hexdigest": "e1df95e8030d659ee05d448dda0526e2", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0001BC2388", "ncbi_nrp_id": "WP_012855642.1", "uniref100_id": "UniRef100_D1J8F6", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2388", "RefSeq:WP_012855642.1", "UniRef:UniRef100_D1J8F6" ] }, "ips": { "uniref100_id": "UniRef100_D1J8F6", "uniref90_id": "UniRef90_D1J8F6" }, "psc": { "uniref90_id": "UniRef90_D1J8F6", "gene": "Apt", "product": "adenine phosphoribosyltransferase", "ec_ids": [ "2.4.2.7" ], "uniref50_id": "UniRef50_Q9RFQ2", "cog_id": "COG0503", "cog_category": "F", "kegg_orthology_id": "K00759", "go_ids": [ "GO:0003999", "GO:0009116" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RFQ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RFQ2" ], "product": "Adenine phosphoribosyltransferase" }, "genes": [ "apt" ], "id": "GLJLFKCOHC_829", "locus": "GCA013368035_00821" }, { "type": "cds", "sequence": "contig_1", "start": 470028, "stop": 470267, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTGGATGAAAACGGCAAAAATTGTTATGTTTTAGAAATAAATAACAAACTATTTGTAATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGA", "aa": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIP", "aa_hexdigest": "9227c53fcb884f46403e9711cc4e8abd", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8967.135899999994, "isoelectric_point": 5.212613105773924 }, "id": "GLJLFKCOHC_830", "locus": "GCA013368035_00822" }, { "type": "cds", "sequence": "contig_1", "start": 470274, "stop": 470648, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAACAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCATTTGGCGGAGTAAATGTAACTCCTTTTGATCTAGCCGGAGGATTGCCTGGACAACTTGGGTTCAATTTCGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAACAAAAAGGAATTACAACTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGA", "aa": "MKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIQQIINNKKELQLLIMDSSRSAFPGRSIEKL", "aa_hexdigest": "c211546d96dcb2aa95dca5bf55cfb2a6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13803.850099999985, "isoelectric_point": 9.559452629089353 }, "id": "GLJLFKCOHC_831", "locus": "GCA013368035_00823" }, { "type": "cds", "sequence": "contig_1", "start": 470727, "stop": 471683, "strand": "+", "frame": 3, "gene": "rnjA", "product": "mRNA degradation ribonuclease J1/J2", "db_xrefs": [ "COG:COG0595", "COG:J", "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8", "UniRef:UniRef90_D1J8F7" ], "nt": "ATGATAACTGCACATGAAATTTTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAATCCAAATTCTCAATTTCCGGAATTCGTTGAATATCAAACTGCAAATAATGTAAAAAATGCTGTTATTCTTGTAACAGGTGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGATAATGATGTTTATTTAAAATTGAAAAATGATGATGCAGTCATTATAATTGCTCCGCCAGTTAACGGATTAGAAGTAAATTATGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTGCTAAATTAGATATTTTTAATATTATTAAAGCTTTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCTAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCCGAATTCAACGATACAAATTTAATTTCTCAAAAACATGGAATTAGAAATGTAGGTGATGTTATTATTGATGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACTTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTTGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAAAGAAAAATCGCCAAAACTATCGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAA", "aa": "MITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI", "aa_hexdigest": "6d31e7ac111009a0ff6504458270a352", "start_type": "ATG", "rbs_motif": null, "genes": [ "rnjA" ], "truncated": "5-prime", "pseudogene": { "start": 470427, "stop": 471683, "observations": { "mutation": [ 470646 ], "stop": [ 470646 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8F7", "query_cov": 1.0, "subject_cov": 0.5771324863883848, "identity": 1.0, "score": 571.0, "evalue": 3.86e-201, "gene_start": 1, "gene_end": 318, "reference_start": 234, "reference_end": 551, "reference_sequence": "MNKINFFALGGLDENGKNCYVLEINNKLFVINSGTKIPINSHNGIDTLICNYEFLEKRQKDIEGLFLTDVRNETFSAIPWLLMKIKNLKIYTSAFNKIVLLDRISKYNIGSNTFEVKTINKKTAFGGVNVTPFDLAGGLPGQLGFNFETPISNILFMTNFVDGDLGPYGKTDLQQIKQIINNKKELQLLIMDSSRSAFPGRSIEKLWVSRKIEYKFKETSDNQRIIVGLYDEDMITAHEILILAKRYNRPVITYGLAYSQLIKLVEKVNPNSQFPEFVEYQTANNVKNAVILVTGAIDRLYTRFARISSDNDVYLKLKNDDAVIIIAPPVNGLEVNYAITLDDIARHTANLIELGNDQYYSCNPAKLDIFNIIKALKPKFFIPIQGLYRYQVVATQVARSAGMNISNCLVLQNGKVAEFNDTNLISQKHGIRNVGDVIIDGYGIGDISHEVINERENLARDGVIALSSLIDYKKKKPINELQISSYGIITKDNKEVIHKIINDLFKKEFENKQTKEINLKEIQEKLRKSIKRKIAKTIDKEPMIVITLYEI" }, "paralog": false, "identity": 0.9952153110047847, "score": 767.0, "evalue": 5.63e-281, "description": "Internal stop codon at 470646. Nonsense mutation around 470646.", "psc": { "uniref90_id": "UniRef90_D1J8F7", "gene": "RnjA", "product": "mRNA degradation ribonuclease J1/J2", "uniref50_id": "UniRef50_A0AAJ5NJJ8", "cog_id": "COG0595", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8F7", "UniRef:UniRef50_A0AAJ5NJJ8", "COG:COG0595", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0AAJ5NJJ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AAJ5NJJ8" ], "product": "Ribonuclease J" } }, "id": "GLJLFKCOHC_832", "locus": "GCA013368035_00824" }, { "type": "cds", "sequence": "contig_1", "start": 472183, "stop": 472728, "strand": "+", "frame": 1, "gene": "ftsK", "product": "Cell division protein FtsK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8F8", "UniRef:UniRef90_D1J8F8" ], "nt": "GTGATTGGAACATTTATTAATTCAATATTAAATATTCCTGGAAAAAAACTCGGTTTTTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGCTTATCTGTCATTTCATTTTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAAGAAGAGGATATTGAAATAATCGATAACAAAGAAGTCGTAAATAGCAATATTCAAATTTTAGAGGCTAATTCTAACTCAAATAAAAATATAGACTCAGCACAAAATTCAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGATGAAAAGGAAATTGAAGAAGATTCAAAGCAAAAAGACAACAATGAGTTAGAGCCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGATCCAACCCCGCAAAACCTAGATAATTCAAATAAAAATAAAGAGATTAAAAAAGAAGATAAGATCTATAAAATCATTGAAGATGAAGAAGATTTATTCTAA", "aa": "MIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSAQNSNQNNNLDLDPFDDEKEIEEDSKQKDNNELEPKEAIDSPSNENSIDPTPQNLDNSNKNKEIKKEDKIYKIIEDEEDLF", "aa_hexdigest": "8d128f6afacdb8c1da45a1f42529aa44", "start_type": "GTG", "rbs_motif": null, "genes": [ "ftsK" ], "truncated": "5-prime", "pseudogene": { "start": 471883, "stop": 472728, "observations": { "mutation": [ 471952, 471988, 472123 ], "stop": [ 471952, 471988, 472123 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8F8", "query_cov": 1.0, "subject_cov": 0.5419161676646707, "identity": 0.9940000000000001, "score": 272.0, "evalue": 7.47e-89, "gene_start": 1, "gene_end": 181, "reference_start": 154, "reference_end": 334, "reference_sequence": "MKVAKEKQTKIKNQKQYFFSRDRVLLWTIILLVTLFFLVISFLNVPWLTTLHSYSFNVLFGMFSILFYLLIILLSIWKIFNLKSTYKYWIFNFNLGRLIFFFINIIILGSVIYYTIHQVEHNSKNFFAKYFSSWFVDFSNSSNYLLPQKYTPGVIGTFINSILNIPGKKLGFSIAYIISIVLLSLSVISFFISNSFFKMIFSPKSKKNKEKLQNQSDLKIKEEDIEIIDNKEVVNSNIQILEANSNSNKNIDSAQNSNQNNNLDLDPFDDEKEIEEDSKQKDNNELEPKEAIDSPSNENSIDPTPQNLDNSNKNKEIKKEDKIYKIIEDEEDLF" }, "paralog": false, "identity": 0.9893238434163701, "score": 492.0, "evalue": 4.21e-178, "description": "Internal stop codon at 471952, 471988, 472123. Nonsense mutation around 471952, 471988, 472123.", "psc": { "uniref90_id": "UniRef90_D1J8F8", "product": "Cell division protein FtsK", "uniref50_id": "UniRef50_D1J8F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8F8", "UniRef:UniRef50_D1J8F8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8F8" ], "product": "Cell division protein FtsK" } }, "id": "GLJLFKCOHC_833", "locus": "GCA013368035_00825" }, { "type": "cds", "sequence": "contig_1", "start": 473093, "stop": 474043, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81", "UniRef:UniRef90_UPI00215D7B81" ], "nt": "ATGAAAAACAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCATTAACATTGATTGCATCCGCCATCACAATACCCGTTGTAATTTCATCTTTAATTATAAATAAGAATAATTGGGCTAACAAATACTATAGCCAAAGATCAGAGGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCTTAGAGGATCAAAATAATTTAAATAAGTTATTGAATAAAATTAATTTAACCAGCAAAAATAGTTCTGCTGATTTTAAAAACTCATATAATTTGCTTAACAAGACAAATCTTGATATCAACAAAAAGATAAATATTAACAAAAAAGAATTTGATAAAACTTATGCTGATATTTTGCTTTTAAGCTCACAGTTGCAAGATTTATTCAATAAAATTATTGATTTAAAATTGAACAACAATATAAAAGAAGATGTTGATAGCTTATTACAAAGAGTTGAAAAAGATCTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGATATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTGAATTCATATTATAAATTAATTAGCAATAAAGATAAAACATATAAAGGCATTGTCTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATAGCAATGAAATAACCGATCTTAAAAAGAATTTTAAAAATTTCCAATCAGTTGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAAGAAATTAAAGAAGAAATTGTTGAACACAGAAAGCTAAGCCAAAAGATAAAAAAAGATGGAAATGATATTCTAAATAATGATTATGATAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCGAAATCATTTTTATTAATGTGA", "aa": "MKNKKSKKIVVALSLTLIASAITIPVVISSLIINKNNWANKYYSQRSEASHLVNLIEKNKHFYYFALEDQNNLNKLLNKINLTSKNSSADFKNSYNLLNKTNLDINKKININKKEFDKTYADILLLSSQLQDLFNKIIDLKLNNNIKEDVDSLLQRVEKDLNKCRKIDENINLIDIKEIYAHSSKTIIDVSNQLNSYYKLISNKDKTYKGIVSEIKDLKKDYFNKIEDGEEYYKLYSNEITDLKKNFKNFQSVEDYNNYINYANSKIKEIKEEIVEHRKLSQKIKKDGNDILNNDYDKSALIRNSYFQEPKSFLLM", "aa_hexdigest": "b53e80ab622be671653073f3d6af9fdf", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00215D7B81", "query_cov": 0.9683544303797469, "subject_cov": 0.8116710875331565, "identity": 0.9079999999999999, "score": 464.0, "evalue": 1.49e-161, "valid": true, "uniref50_id": "UniRef50_UPI00215D7B81" }, "pscc": { "uniref50_id": "UniRef50_UPI00215D7B81", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D7B81" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 37074.90300000002, "isoelectric_point": 9.211710166931152 }, "id": "GLJLFKCOHC_834", "locus": "GCA013368035_00826" }, { "type": "cds", "sequence": "contig_1", "start": 474365, "stop": 474685, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D844A", "UniRef:UniRef90_UPI00215D844A" ], "nt": "TTGTTTTATGACAAGGTTATTCAAGACCCAAAAACTAACGAAATTAATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCGGAACAAGATATTAGATATTTACACTACCATTTTGGAAGTCTCATAACTCAAAAGGTTAATGAAATAATTAAGCATTATAACGAAAAAGCAAAAGCCTTATTAGAAAAAATAAAAAACAATAATAGTTACGAAACACAAAAAAATAGAATAGAAAAATTACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGCGATTTCAATGATTACGACAAATACTATTGGTGA", "aa": "MFYDKVIQDPKTNEINEEKLMKLDDIHFDPEQDIRYLHYHFGSLITQKVNEIIKHYNEKAKALLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYDKYYW", "aa_hexdigest": "7ca56c534c26a0b3767972787f6653e7", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 474162, "stop": 474829, "observations": { "deletion": [ 474186 ], "stop": [ 474192, 474684 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D844A", "query_cov": 1.0, "subject_cov": 0.4774774774774775, "identity": 0.981, "score": 209.0, "evalue": 5.73e-67, "gene_start": 1, "gene_end": 106, "reference_start": 69, "reference_end": 174, "reference_sequence": "MKTFQTLFYTWVIYNNKIRIIKEFFFKDKKENEEFNSFYYKDKDIDNQLNSLLNEEREFKNRQERLVRLFYDKVIQDPKTNEIDEEKLMKLDDIHFDPEQDIRYLHYHFGSLITQKVNEIIKHYNEKAKALLEKIKNNNSYETQKNRIEKLLAKNITYVNNFRGDFNDYDKYYWWTYKFQKDSNGDIILSPNSKIPDFYKNEIEVAQYLHELDKILDNLKNI" }, "paralog": false, "identity": 0.972972972972973, "score": 380.0, "evalue": 1.15e-136, "description": "Internal stop codon at 474192, 474684. Frameshift due to deletion around 474186.", "psc": { "uniref90_id": "UniRef90_UPI00215D844A", "uniref50_id": "UniRef50_UPI00215D844A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D844A", "UniRef:UniRef50_UPI00215D844A" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D844A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D844A" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 12996.498799999981, "isoelectric_point": 6.414473533630371 }, "id": "GLJLFKCOHC_835", "locus": "GCA013368035_00827" }, { "type": "cds", "sequence": "contig_1", "start": 475072, "stop": 475206, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAATGAGAAAGAAATTAATTGCTTTATCTGTGGCTTTTGGACTTATAGCGCCTATTTCAGGAGCTCTTATGATTTCATGCAAAAACGAAAAAAGTAATGCTGAATATCAAAAAGAACTGCAAAATAAATGA", "aa": "MEMRKKLIALSVAFGLIAPISGALMISCKNEKSNAEYQKELQNK", "aa_hexdigest": "99ef47a538d0d6e31658c42e4fa507d0", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4897.800800000001, "isoelectric_point": 9.360438728332518 }, "id": "GLJLFKCOHC_836", "locus": "GCA013368035_00828" }, { "type": "cds", "sequence": "contig_1", "start": 475333, "stop": 475878, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAGAATTTTATTGCAAAAATTAATGAAACTACACAAGATATAAATGCACAAATTTCAAAATTAAAAAACGAAAACGGAAATACAAACTCTGAAAACAATAGCCAAAATGATTTATTAAAAAAATTAAAAGTAGAATTAGAAATAGCAACTACAAAACTTAACATGCTACTTTCAGCAAATGAAAGTAAAAACATCTCAATAAAAGAAGAAGCTAAAAACATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAACAAGAATATAAAAATCAAATTCAAAAAGTAAATGAAACCATCAATAAAATTGAAGAAGAAATAGCAAATTTAGAAAAAAGTCCAAAATCAAAAGAATTTACTAAATTAAAAGAATTAAGGGAAAAACTAACAAAATACTTAGATAATAATTTCTCTGACTTTGCTTATAAAGATTTAAGAGCTGAAATTAAAGAATTAATTCAAAAACAAGAAAACGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTTCACAAATAAATGATGCCTTTTGA", "aa": "MKNFIAKINETTQDINAQISKLKNENGNTNSENNSQNDLLKKLKVELEIATTKLNMLLSANESKNISIKEEAKNILNAANKALEKSNEQEYKNQIQKVNETINKIEEEIANLEKSPKSKEFTKLKELREKLTKYLDNNFSDFAYKDLRAEIKELIQKQENVSIVSNEKEIKSAISQINDAF", "aa_hexdigest": "e257036ca5e84b43e4ab78c86181d0d2", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20779.215100000005, "isoelectric_point": 6.484044456481934 }, "id": "GLJLFKCOHC_837", "locus": "GCA013368035_00829" }, { "type": "cds", "sequence": "contig_1", "start": 475993, "stop": 476835, "strand": "+", "frame": 1, "gene": null, "product": "P75 protein precursor", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RLP9", "UniRef:UniRef90_UPI000CD67922" ], "nt": "TTGAATACCGCTATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACCAAAATTTCTAATGTAGAAAAACAAAATTATATTGAAGAAGTTAAAAATACTATAAACTCTTTGTTAAACAAATATAATAATAATATAAATAAAAATAAAGATCAAATTTATGAATTAAGTGGAGACATAGAACAATTAAATAAATTAAATCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAACAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCTAAAAAAGCAATTTTACTAGAAACATTAAGAGATACCATTTCGGAATTTCTTGAATTTTATTTACAATTAACCCATTATCTAAGAATCTTTTTTGACAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTAATGATTATCTAAACAAAATTAAAAAAATTACAGAAAAAAATAGTCAAACCAGAGGAAGCGAACCAACATTTGATGAATATATTTCAGCCTTTAAGGCACAAGAAGAAATTTTTAAAATTATAAAATCTAGTGATCTAAAATCAGCAGATGTTAACATATTAGAAAAATTGACAAATAATTTAAAAGAAATTAAAAAAGAAAAGAAAAATGATTACAACTTGGTTGTTACTTCTTATTTAAAAGAAAAAATAGAAGAAGCAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATATTGAAAGTCAAAATATTATTGAAACGTTTGCTTTATTGCAAAAATTAGAACTTGAATTTGCTTATATTAATTGA", "aa": "MNTAIFENIETTNERNLLNKYIKTNLTKISNVEKQNYIEEVKNTINSLLNKYNNNINKNKDQIYELSGDIEQLNKLNQIENNYVIDKVKLLNLLNKANTIKNEGSFAKKAILLETLRDTISEFLEFYLQLTHYLRIFFDKNNDIIKLLPADSQTKINDYLNKIKKITEKNSQTRGSEPTFDEYISAFKAQEEIFKIIKSSDLKSADVNILEKLTNNLKEIKKEKKNDYNLVVTSYLKEKIEEAITKNESIIAKKSNIESQNIIETFALLQKLELEFAYIN", "aa_hexdigest": "53c377995f0d019e4f939d120f3ad206", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 475693, "stop": 477036, "observations": { "mutation": [ 475876, 476833, 476935 ], "stop": [ 475876, 476833, 476935 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000CD67922", "query_cov": 1.0, "subject_cov": 0.43143297380585516, "identity": 0.893, "score": 455.0, "evalue": 8.93e-155, "gene_start": 1, "gene_end": 280, "reference_start": 304, "reference_end": 582, "reference_sequence": "MKMRRKLIALSVAFGLIAPISGALMISCKNEKSNAEYQKELQNKWTELKNLLNSNSFKEVESKLDLSKLNALKEEYKTINSQSNVSTLKNFIAKINETAQDINAQISKLKNTNGNTNPENNSQNNLKVELEIATKKLDMLLSSNEGKNISIKEEAKKILNAANKALEKSNEQEYKNLIQKVNETIDKIEKEILNLEKGPKSKEFTKLKELREKLTNYLDNNFSEFAYKDLRAEIKELIQKQENVSIVSNEEEIKSAISQINDAFWIASIKKSILDKQFLYLENKPKSELDIKFLAAYDELSQALNTSIFENIETTNERNLLNKYIKTNLTKILDVEKQKYIEEVKNTISSLLNKYNNNINNNKDQIYELSRNIEQLNKLNQIENNYVIDKAKLLNLLNKANTIKNEGSFAEKAILLDELKDSVSEFLEFYLQLTHYLRIFFDKNNDIIKLLPADSQTKINDYLDEIKKITEKNSQTRGNEPTFDEYISAFKAQEEIFKIISSDLKSADVNILEKLTNNLKEIKKEKKNDYNLVVTSYLKEKIEETITKNESIIAKKSNVESQNIIETFALLQKLELEFAYINWTELNLIAKIKNKLELLIKINKIDSNEEAKILKPWTDSFKSSKKPEIFELENILKELSKSLKELINK" }, "paralog": false, "identity": 0.9038031319910514, "score": 763.0, "evalue": 2.09e-278, "description": "Internal stop codon at 475876, 476833, 476935. Nonsense mutation around 475876, 476833, 476935.", "psc": { "uniref90_id": "UniRef90_UPI000CD67922", "product": "P75 protein precursor", "uniref50_id": "UniRef50_Q9RLP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000CD67922", "UniRef:UniRef50_Q9RLP9" ] }, "pscc": { "uniref50_id": "UniRef50_Q9RLP9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9RLP9" ], "product": "p75 protein" } }, "id": "GLJLFKCOHC_838", "locus": "GCA013368035_00830" }, { "type": "cds", "sequence": "contig_1", "start": 476823, "stop": 477032, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAATTAGTTCATTTAAAGATTTGCTTAATTCTTTTAAGATGTTTTCTAATTCGAAAATTTCTGGTTTTTTTGGACTATTAAGCAAATCTGTTCAAGGTTTTAATATTTTTGCTTCTTCATTTGAATCTATTTTATTGATTTTTATTAATAGTTCTAATTTATTTTTAATTTTAGCAATCAAATTTAATTCAGCTCAATTAATATAA", "aa": "MLISSFKDLLNSFKMFSNSKISGFFGLLSKSVQGFNIFASSFESILLIFINSSNLFLILAIKFNSAQLI", "aa_hexdigest": "65725e437220808f04d80292eedb2981", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7691.008799999992, "isoelectric_point": 9.82448215484619 }, "id": "GLJLFKCOHC_839", "locus": "GCA013368035_00831" }, { "type": "cds", "sequence": "contig_1", "start": 477217, "stop": 477534, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D96F05" ], "nt": "ATGATTAATGATATGTTTGAAAAAACTAGTCAAGCTAGTGTTTATAAAATAAAAACATTCTTGCCTGATAAAACTATTCGTGATGAAAGAAAATTAAATTTTGACAGAAGTCTTCCTTTATTTAAATTATTTAGCAAAGTACGCGTGAATTTTAATACATTTTTAGGGGGACCAAAAACTAAAGGAATTTATGAACAAGATGGAAAATTAAGATTTGAAGGATTTATTCAATTTTATGATCCAGAAATTCCTTATTATAAATATATTGGAACGGCAAATCCTATAACCATCGACGAAATAACAATTAAAGCCCAATAA", "aa": "MINDMFEKTSQASVYKIKTFLPDKTIRDERKLNFDRSLPLFKLFSKVRVNFNTFLGGPKTKGIYEQDGKLRFEGFIQFYDPEIPYYKYIGTANPITIDEITIKAQ", "aa_hexdigest": "239885d0e2f217981b48d7553244bb4b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D96F05", "query_cov": 0.819047619047619, "subject_cov": 1.0, "identity": 0.884, "score": 155.0, "evalue": 1.09e-47, "valid": false, "uniref50_id": "UniRef50_UPI0034D96F05" }, "pscc": { "uniref50_id": "UniRef50_UPI0034D96F05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034D96F05" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12343.109099999987, "isoelectric_point": 9.300160789489745 }, "id": "GLJLFKCOHC_840", "locus": "GCA013368035_00832" }, { "type": "cds", "sequence": "contig_1", "start": 477637, "stop": 478083, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCCACCAAGTGATGAATTTTTAGAAATTGAAAAAGAATTTCAAGACCAAAAGAAGCAAATTGAATCTAAACTAAATGAAATTTGCTCTTTAAAATTCAATAAAACATACAGTGGGCAATTAGTTGATGAATCAAATGAATCTATAAAGAAGAGAATTAGTCAAAGAGTTAATGAAGTTTATGGCGATTATCCCGGCTATGAAACAAAATATTATTATAGAAGAGCATATAATGAAAGAAATAAAGAAGATTGCAAGAAATTTATAAACCAACTTAAAAATATTTATAAGATAGTTGATATGTACGCTAATTATTTAAAAGAAGTATTCAAATATATTGAATCAGAAGATGCAAAATTAAGTGATGAAATAAAACAAGAGATAATGGAAAAATATGTTTCAGATCCAACAGAATTAAGTAGAAGCAATATCGAAAATTTAAATTGA", "aa": "MPPSDEFLEIEKEFQDQKKQIESKLNEICSLKFNKTYSGQLVDESNESIKKRISQRVNEVYGDYPGYETKYYYRRAYNERNKEDCKKFINQLKNIYKIVDMYANYLKEVFKYIESEDAKLSDEIKQEIMEKYVSDPTELSRSNIENLN", "aa_hexdigest": "93bdf67aec4e24b330e783241ba2376e", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17823.783100000004, "isoelectric_point": 5.1498628616332995 }, "id": "GLJLFKCOHC_841", "locus": "GCA013368035_00833" }, { "type": "cds", "sequence": "contig_1", "start": 478390, "stop": 478944, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAAGTCTAAGCAAGAATTACAAAATCTTATTGATTTTAGCGCTGACCAAGGAATTGAAAATTCAAAAGCAAAAGAAATTTTAGATTCAACAAACATTGAAGAAAATTCTTCAATTAGTGAGGTTAAAGAAAAAACAAACAAAATTATTAAAGCTAAACAAGATCTTCAAAATTTAATAGAAGATACCAAAAATAAAGCAAAAAATGAGTTTGATATTAAAAAACAAGAATTGAGAGTACTTCTAGATTCAATAGACAATAAGATTAATAAACAAGACGAAGAAGAAAAATATAAGAATATTGAAGTTAAAGATAATGATTTAGTTAAAGACATTAAAAATAAGACTAATGAAATTTCAAAAGCAATTAAATCATTGAATAAAAAAATTAATGAACAAAAAAATAAACAAGAACTTGAACAAGCAAAACAAGATTTTGAAAAAATAAAAAGAGAACTACAAGAATTAATTGCAACCCCTGATGCAAAAGAAGTAGAAAACATTGATGATATAAACAAAACCTTGAATGAAGCAAACATTGCCGAAGATTGA", "aa": "MKKSKQELQNLIDFSADQGIENSKAKEILDSTNIEENSSISEVKEKTNKIIKAKQDLQNLIEDTKNKAKNEFDIKKQELRVLLDSIDNKINKQDEEEKYKNIEVKDNDLVKDIKNKTNEISKAIKSLNKKINEQKNKQELEQAKQDFEKIKRELQELIATPDAKEVENIDDINKTLNEANIAED", "aa_hexdigest": "86ff7ba26e760dbdb4feb4672cb43db6", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21340.689200000008, "isoelectric_point": 5.114452171325683 }, "id": "GLJLFKCOHC_842", "locus": "GCA013368035_00834" }, { "type": "cds", "sequence": "contig_1", "start": 479116, "stop": 479301, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGAAAAAAAATTTATCATTACAAATTTTAGCGTTGTGTGTAGCAGCGGGCTCTGTGCCTGTTTTAGCATCTAAATGTACCGTAACTGTAAAAGTTAAAGAAAAGTTTAATGCTCTAAAATCACAACTTCAAAATTTAATTTCAAAACTAAGTGATGAAGAACAACAAGAACTTAATGAATGA", "aa": "MKKKNLSLQILALCVAAGSVPVLASKCTVTVKVKEKFNALKSQLQNLISKLSDEEQQELNE", "aa_hexdigest": "4a25dd07e169d330eef575d4d9523fbb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6700.840199999999, "isoelectric_point": 9.064206504821776 }, "id": "GLJLFKCOHC_843", "locus": "GCA013368035_00835" }, { "type": "cds", "sequence": "contig_1", "start": 479515, "stop": 480063, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAATGATATTTGTAAAGAAATAATTAAACAGGAATTTTTAGAAAAAAATTCAAATAACAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTATTGCTGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATAGGTTTTTGTTTTTTTTCATTTTTTGCTAACAATAAGAAAATAATTAAATCATTTGAAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCTTTAGGCAAGGCAAGACAGGCATACGTTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAAGCAAAATGGGGATAAAAAAAATATCACAATACGATGCTAAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGA", "aa": "MEDQKDFNIIDSEIAAFGNVKKIFDENINNINDICKEIIKQEFLEKNSNNKRIEEAKKQYEIVRDSNERIIAEKKLKKISGRKFANCIIFILFFILIGFCFFSFFANNKKIIKSFEEFRNNEQLKIDEALGKARQAYVETFSLQTLEDIKNKILSKMGIKKISQYDAKVGDFSRSFIYDKNF", "aa_hexdigest": "f951a34a3d21b3d8f542a5c3ea7f1081", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21445.501199999995, "isoelectric_point": 8.96937351226807 }, "id": "GLJLFKCOHC_844", "locus": "GCA013368035_00836" }, { "type": "cds", "sequence": "contig_1", "start": 480487, "stop": 481395, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032", "UniRef:UniRef90_UPI00215DA032" ], "nt": "ATGAATCAAGAAAACATGCCGGTATTTTCAATGATAAAAAATGGAAATTGGTTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAGGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAATTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATATTCAAAATTGCCCCCTAGACATTCACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTTATAATGACCCAGAATCTTTGATAATATCGGCTACATTTGATTTGATTTCATATACAGCTAGAGACGCAATTGCAATTGAATATACACACGGTTACGCTGTTGAAGTACCATATGTGGCATATGATGAATTTAGAGAACATAAAGCAATAATTGTTGTTCCTTATAATATAAAAACAGAAAATGCCAAAATATTTGCAAATAGTCTTATTGCTAATAATATTTATGTAAGCGACGATCGCATAGATGAAGAATATAGCAAAATTATTCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAAGCTTATAGATGAATTATTTAGTCAGATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAGTTAAAAAGCTTAATAATTTTTATTAA", "aa": "MNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLNSKIIECLLPIFNFISLVYTNKYIATENLEKTENRYEQDLVVKQNNNIQNCPLDIHSYYRDMTLYSGFYKFNKHADITPICEFKNLYNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLVKKLNNFY", "aa_hexdigest": "14f4d9888b225c41dc9d5204b259752f", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 480187, "stop": 481395, "observations": { "mutation": [ 480202, 480214 ], "stop": [ 480202, 480214 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215DA032", "query_cov": 1.0, "subject_cov": 0.6425531914893617, "identity": 0.99, "score": 590.0, "evalue": 4.16e-210, "gene_start": 1, "gene_end": 302, "reference_start": 169, "reference_end": 470, "reference_sequence": "MGIKKISQYDAEVGDFSRSFIYDKNFWNFNSISQYKLRNSYIYDIFYTCKWMKNVVTKASITVTEEDANGNTYWETYWAYHTEPTPVVEYFRSLTIPSNYKQKLYFEIIENNRSEKEVKRLLKNEGFHLENKEVYQNFDFAFSDDQKIDFLTYFDVRTQQNLINYAQYMNQENMPVFSMIKNGNWLVSKNTIQSSSLLFEQVQDWMLSVLDLTEELTVDFVTEFLSSKIVECLLPIFNFISLVYTNKYIATENLEKTGNRYEQDLVVKQNNNIQNCPLDIHSYYRDMTLYSGFYKFNKHADITPICEFKNLYNDPESLIISATFDLISYTARDAIAIEYTHGYAVEVPYVAYDEFREHKAIIVVPYNIKTENAKIFANSLIANNIYVSDDRIDEEYSKIIQKNKISFNLESLKDFDEFKKNIKLIDELFSQIPEIKKIPILLDNRGMAFFINEPKLFDINLLVKKLNNFY" }, "paralog": false, "identity": 0.9875621890547264, "score": 799.0, "evalue": 3.49e-295, "description": "Internal stop codon at 480202, 480214. Nonsense mutation around 480202, 480214.", "psc": { "uniref90_id": "UniRef90_UPI00215DA032", "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215DA032", "UniRef:UniRef50_UPI00215DA032" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215DA032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DA032" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 35435.1183, "isoelectric_point": 4.861575508117675 }, "id": "GLJLFKCOHC_845", "locus": "GCA013368035_00837" }, { "type": "cds", "sequence": "contig_1", "start": 481482, "stop": 482039, "strand": "+", "frame": 3, "gene": "lemA", "product": "Protein LemA", "db_xrefs": [ "COG:COG1704", "COG:M", "GO:0005886", "KEGG:K03744", "SO:0001217", "UniRef:UniRef50_P43054", "UniRef:UniRef90_P43054" ], "nt": "ATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAGCCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATCTTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATTGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATAAGAGGTGCTTTAAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACTGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGA", "aa": "MLFDSRTPQSSEGFKPNVDNSIKKPIPTGVEKFFFILFFILTIGIFYFVYVGRKNELMRDQNEIQNASSLIQAAEKRRRAVLIKMMDSLIGYKNFENETLTKITQYRSKLSNIDVDKTSPVELKSQIDSIRGALNFQFEQYPDLKASKLYLQFSTEISMQEDEIYATIRNYNMIATSFNSKIYTF", "aa_hexdigest": "181baf512e89f9176479d877cbeb4d28", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_P43054", "query_cov": 1.0, "subject_cov": 0.8447488584474886, "identity": 0.995, "score": 357.0, "evalue": 3.14e-124, "valid": true, "gene": "lemA", "product": "Protein LemA", "uniref50_id": "UniRef50_P43054", "cog_id": "COG1704", "cog_category": "M", "kegg_orthology_id": "K03744", "go_ids": [ "GO:0005886" ] }, "pscc": { "uniref50_id": "UniRef50_P43054", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43054" ], "product": "Protein LemA" }, "genes": [ "lemA" ], "id": "GLJLFKCOHC_846", "locus": "GCA013368035_00838" }, { "type": "cds", "sequence": "contig_1", "start": 482227, "stop": 482361, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGCTCAATAGGATTTAAGGGTTTACACCATTTGCTATGA", "aa": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLL", "aa_hexdigest": "8d4f09ee56f4b74af8dfcccb6640f0f0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5048.840599999998, "isoelectric_point": 6.81854190826416 }, "id": "GLJLFKCOHC_847", "locus": "GCA013368035_00839" }, { "type": "cds", "sequence": "contig_1", "start": 482392, "stop": 482637, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAACAATGTAATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACTGGAATTCACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGTGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGGTTGCACGGTGTTGGCGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGA", "aa": "MAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEV", "aa_hexdigest": "db565570dd8e84d5374519d63bf2fa94", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D3B81C", "query_cov": 1.0, "subject_cov": 0.84375, "identity": 0.593, "score": 100.0, "evalue": 4.92e-26, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_Q706I4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 76, "aa_cov": 0.9012345679012346, "hmm_cov": 0.8157894736842105, "evalue": 4.435510570208102e-11, "score": 38.64239501953125, "start": 5, "stop": 77 } ], "seq_stats": { "molecular_weight": 8404.4133, "isoelectric_point": 6.25185718536377 }, "id": "GLJLFKCOHC_848", "locus": "GCA013368035_00840" }, { "type": "cds", "sequence": "contig_1", "start": 482773, "stop": 484011, "strand": "+", "frame": 1, "gene": "gyrB", "product": "DNA gyrase subunit B", "db_xrefs": [ "COG:COG0187", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0005524", "GO:0005694", "GO:0005737", "GO:0006261", "GO:0006265", "GO:0034335", "GO:0046872", "SO:0001217", "UniRef:UniRef50_P43053", "UniRef:UniRef90_P43053" ], "nt": "ATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAGGGAGTTTTTAATGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATCGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACACACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATCGCTACAAATAAAATTCTTTCAGACTCACTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCAGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCGGCCGGAGGTTCTGCTAAAATGGGTAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGAACAGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCACACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCACTATATAAAATAACTAGCGGAAAAAATGTTGAATATGCATATAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGA", "aa": "MEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQL", "aa_hexdigest": "9717852de3b2dd115eeb6f48d4d09056", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI000D1D9E0B", "query_cov": 0.9053398058252428, "subject_cov": 0.8923444976076556, "identity": 0.517, "score": 389.0, "evalue": 8.47e-130, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_F5VUB3" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 482473, "stop": 484173, "observations": { "mutation": [ 482635, 484009 ], "stop": [ 482635, 484009 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43053", "query_cov": 1.0, "subject_cov": 0.6358024691358025, "identity": 1.0, "score": 810.0, "evalue": 2.78e-292, "gene_start": 1, "gene_end": 412, "reference_start": 183, "reference_end": 594, "reference_sequence": "MDKIEEIHKYNADNIQILEGLEAVRKRPGMYIGSIGFKGLHHLLWEIVDNSVDEAMAGFATEIKIKLYPNNVIEVEDNGRGMPTGIHSGTKKSAVETILTVLHAGGKFDGSNYKVSGGLHGVGASVVNALSSEFEVWVKRDGKLHYQQFRNGGIPVKPLEVIGNVSEVETGTTIKFHPDYTIMEKENFDFDTIIDHSKQIAYLNKGLKITVENVEKNIIKVFCFEGGLIDYVKELNKGKKLIVPEVIYAEGVFNDKNFTNGQDVIVEVAMQYNEAYTNSIVSYANNIQTIDGGTHEQGFYDALVRIYNNYAETNKLFKTSSEKITREDVKEGLVAIISIKHTDPIFEGQTKGKLENKDARIATNKILSDSLERYLNENPEIARAIIEKCLLSQHTRLLEIKAREASRKGNGLDLGNLPGKLADCSSKNAEIRELFIVEGNSAGGSAKMGRDRSIQAILPLRGKVINAEKNSFASVLSNKEIATMIHALGTGINTEFDINKLKYHKIIIMTDADVDGAHITTLLLTFFYRYMKPLIEYGFVYLAQPPLYKITSGKNVEYAYNDLQKEQIMAKLEDKRNVAIQRYKGLGEMDPEQLWETTMDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI" }, "paralog": false, "identity": 0.9964664310954063, "score": 1113.0, "evalue": 0.0, "description": "Internal stop codon at 482635, 484009. Nonsense mutation around 482635, 484009.", "psc": { "uniref90_id": "UniRef90_P43053", "gene": "gyrB", "product": "DNA gyrase subunit B", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_P43053", "cog_id": "COG0187", "cog_category": "L", "go_ids": [ "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43053", "EC:5.6.2.2", "UniRef:UniRef50_P43053", "COG:COG0187", "COG:L", "GO:0005694", "GO:0005737", "GO:0005524", "GO:0003677", "GO:0034335", "GO:0046872", "GO:0006265", "GO:0006261" ] }, "pscc": { "uniref50_id": "UniRef50_P43053", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43053" ], "product": "DNA gyrase subunit B" } }, "id": "GLJLFKCOHC_849", "locus": "GCA013368035_00841" }, { "type": "cds", "sequence": "contig_1", "start": 484021, "stop": 484173, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTATTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAA", "aa": "MDPETRKMLQVQIDDAAICDTVFATLMGEEIEPRHDFIQENAKYANNIDI", "aa_hexdigest": "21e2080d0bfa91ac8070ba9604ef6d73", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5754.417799999999, "isoelectric_point": 4.176893043518065 }, "id": "GLJLFKCOHC_850", "locus": "GCA013368035_00842" }, { "type": "cds", "sequence": "contig_1", "start": 484175, "stop": 484360, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAAGCCTCTAACTAATCAAGGTTTCACAAACAAAGTATTTTACGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCATTCTTGCTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGA", "aa": "MLKPLTNQGFTNKVFYDDETNRFIKIKSYDGFNHKSDAFLLNNLDFCPKIFVDNKKELQTE", "aa_hexdigest": "beb625d91cc544b720c1c5a13551fe00", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7235.125100000001, "isoelectric_point": 6.551284980773925 }, "id": "GLJLFKCOHC_851", "locus": "GCA013368035_00843" }, { "type": "cds", "sequence": "contig_1", "start": 484391, "stop": 484615, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACAGATGATATTTTAAAAACTATTGGAAAAAATTTAATCACTCTACATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAGAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATCGACAATTCCGCCCCTGTCCATAATGACCTTTGA", "aa": "MTDDILKTIGKNLITLHNSKLKFYKENQIARRFNIYRKKISSLNREIPILDKYYKKINLFLRNIDNSAPVHNDL", "aa_hexdigest": "97b3f7ad1cf92585f761860836f8eb4f", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8863.256999999994, "isoelectric_point": 9.994098472595216 }, "id": "GLJLFKCOHC_852", "locus": "GCA013368035_00844" }, { "type": "cds", "sequence": "contig_1", "start": 484676, "stop": 484831, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCGATGTTCATTTTGACCTTGCCTATTTTATTGAATCAAGCAATCTAAATGAAAAACAAGAAAAAGTGTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTAGTAAATGCCTTAATTGTGCTATGA", "aa": "MGDVHFDLAYFIESSNLNEKQEKVFLDAYGDDFEPKYLFIHKILVNALIVL", "aa_hexdigest": "7b8a2a13d8a2be708d1059d9310e35ac", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5963.7613999999985, "isoelectric_point": 4.547426414489746 }, "id": "GLJLFKCOHC_853", "locus": "GCA013368035_00845" }, { "type": "cds", "sequence": "contig_1", "start": 484926, "stop": 485129, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGATGATTTACATAGTAGTACATATATTGGAAAACTAGATGATGTTTGA", "aa": "MLKRDCCCGCKSAEFCTCKNKKSCIPNNLYEKIRNVFGYEVFEKLNNLRPYFDDLHSSTYIGKLDDV", "aa_hexdigest": "41eadcc39d14190c748f3075323eae69", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7822.973599999999, "isoelectric_point": 8.167257881164552 }, "id": "GLJLFKCOHC_854", "locus": "GCA013368035_00846" }, { "type": "cds", "sequence": "contig_1", "start": 485493, "stop": 485864, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGATTTCGAATATGTTGCTTTAAACAATAAATATGTAGATCCAGTAAGTCTTTATTTATTTTTAGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTATATTTGATGATTTTATTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATCCCCAATCGTTAAACCCTTTCGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCACTTTGTGTTTACGAGGATGAAGATAGAACCATATGA", "aa": "MDFEYVALNNKYVDPVSLYLFLGIPKEDIIDFFKLDPSIFDDFIFLMRVYNEAMYLNDYSKNNPQSLNPFDSKSLYSNKDFLELNRFIVQKNHNNFDNKLNISKIEKFYFVPLCVYEDEDRTI", "aa_hexdigest": "eec31181de2d60331b8c9fecfcdede4b", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14800.617599999981, "isoelectric_point": 4.578460502624512 }, "id": "GLJLFKCOHC_855", "locus": "GCA013368035_00847" }, { "type": "cds", "sequence": "contig_1", "start": 486273, "stop": 486425, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTTACAGCATATTTATATAACAAATTATTAAACACTGATTTTAACGCTGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAA", "aa": "MICPKDFYKYRIIVHFTAYLYNKLLNTDFNAAKVNTKRINEIFEKLNIKD", "aa_hexdigest": "4d6d525a10d2f40f07be44431cd0d210", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6072.0634, "isoelectric_point": 9.416461753845216 }, "id": "GLJLFKCOHC_856", "locus": "GCA013368035_00848" }, { "type": "cds", "sequence": "contig_1", "start": 486436, "stop": 486918, "strand": "+", "frame": 1, "gene": null, "product": "putative protein MHO_3790", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056", "UniRef:UniRef90_P43056" ], "nt": "ATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGATGTCGACAAGCAAGAAAAAAAATCAATTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAACGCACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAATAAAACTTATAAAATACTTACCCTAGCATCTAAAAGTCTTAATGTTTCTGATATTATGAGCGAAAGCGCAAGATACATGAATATTCCACAAAAATCATTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAACACCAACTTGTTAAAAAATACAAAAATTAATGAGACAAAGGTTACAAAAATAACTAATAGTAACCAAACTATTTTTAAATTAGATTTAGTTCAACTATGA", "aa": "MKKGKAKETKTIDVDKQEKKSIRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKILTLASKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLVQL", "aa_hexdigest": "07ff475e4b21a7c7cbdf77bab12e9a14", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 486436, "stop": 487221, "observations": { "mutation": [ 486916 ], "stop": [ 486916 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_P43056", "query_cov": 1.0, "subject_cov": 0.5970149253731343, "identity": 0.9690000000000001, "score": 288.0, "evalue": 1.58e-96, "gene_start": 1, "gene_end": 160, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKKGKAKETKTIDVDKQEKKSVRIRRVKTGFLFSGILIPLVCVVAIPLSLNKNYSLKRTKYFTDDRTLYSIDKNTIKENNKTYKVITLTSKSLNVSDIMSESARYMNIPQKSFNNSVTYSNKINIRTNTNLLKNTKINETKVTKITNSNQTIFKLDLIQLWNEINSKSNNKISEFDFLSNIESINNAMTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI" }, "paralog": false, "identity": 0.9770114942528736, "score": 501.0, "evalue": 3.79e-183, "description": "Internal stop codon at 486916. Nonsense mutation around 486916.", "psc": { "uniref90_id": "UniRef90_P43056", "product": "Uncharacterized protein MHO_3790", "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_P43056", "UniRef:UniRef50_P43056" ] }, "pscc": { "uniref50_id": "UniRef50_P43056", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P43056" ], "product": "Uncharacterized protein MHO_3790" } }, "id": "GLJLFKCOHC_857", "locus": "GCA013368035_00849" }, { "type": "cds", "sequence": "contig_1", "start": 486997, "stop": 487242, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACTTACTCTTATATAAAAAATAACAAACTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAATTCTTTGAAAATTTTGATGTTGAGATTCGTAAAGAAAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATCGAATTAAACGAATCATTAACCGAAATTAAAAAAATCACTTTTGATACTAAAATTACATTAAAATATGAAATATAA", "aa": "MTYSYIKNNKLFYFDNKQGYSGYIIQKFFENFDVEIRKENLKNKNVNVLEAFISSTIELNESLTEIKKITFDTKITLKYEI", "aa_hexdigest": "3dc4f00d8a053d96587fe4510e7deb2b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9721.00909999999, "isoelectric_point": 7.864836692810059 }, "id": "GLJLFKCOHC_858", "locus": "GCA013368035_00850" }, { "type": "cds", "sequence": "contig_1", "start": 487285, "stop": 487704, "strand": "-", "frame": 2, "gene": null, "product": "DUF885 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "nt": "ATGTTACAATACTTTGGAGCATTAAACGAAGCACAATTACGTAATATGCGTAGAGCAGTTGACCCTGCATACCATGGACAAGGAATTGAAGCAAAATCTGATTTACCTGCAAATGCATCAATTAATGATGTAAGAAGATTTATGTCAGCAAACTCAGCATTAGGTGTTGGTGACATTATGTCTGAATCAAAACGTTACCTAAATCTAGCTGGACAAGCAATTTCATACAATGCTGGTAAAGAAAAGATGCTATCTCTATACGACCAAGTACGTAAACACTTAGGACTATCAAGAAGAGACTTTGTTGAAAAAGAAAACCACAAATACATAAAAGAATTCTTTGACGTTCTATTAAAGAATAGTGCCTTACCTCTAGATACTTTAGAAAAAGTTACAAAGATTCACTACGGTATAAAATAA", "aa": "MLQYFGALNEAQLRNMRRAVDPAYHGQGIEAKSDLPANASINDVRRFMSANSALGVGDIMSESKRYLNLAGQAISYNAGKEKMLSLYDQVRKHLGLSRRDFVEKENHKYIKEFFDVLLKNSALPLDTLEKVTKIHYGIK", "aa_hexdigest": "18a64166ce19bbb7dabfe5108129b1e1", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_A0A3B0P8H0", "query_cov": 0.9424460431654677, "subject_cov": 1.0234375, "identity": 0.611, "score": 164.0, "evalue": 4.8e-50, "valid": false, "product": "Bacterial protein of uncharacterized function (DUF885)", "uniref50_id": "UniRef50_A0A3B0P8H0" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P8H0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P8H0" ], "product": "Bacterial protein of uncharacterized function (DUF885)" }, "genes": [], "id": "GLJLFKCOHC_859", "locus": "GCA013368035_00851" }, { "type": "cds", "sequence": "contig_1", "start": 487978, "stop": 488085, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGACACCACAACCAAATTTACTACGCACGTCAACACTTAGCAAGTCAAAACGGCGAAAATCTAGGTGCAAATGCATTTGACTTTACTTCATACGTTGAAGGTTGA", "aa": "MGHHNQIYYARQHLASQNGENLGANAFDFTSYVEG", "aa_hexdigest": "3ce943058666fa757fc121b9ebcdee3a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3941.1746000000003, "isoelectric_point": 5.727915382385254 }, "id": "GLJLFKCOHC_860", "locus": "GCA013368035_00852" }, { "type": "cds", "sequence": "contig_1", "start": 488119, "stop": 488328, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGTCAACCATCAAGAATGGAAAATCATTCTTCGGTAAAACTGTTCCTGACTACAAAGCAGTAACATATCTATATGAAAGACGTGATCAAGAAGGTGTTGGAGCATACAACTCAGATACACGTATTTTGGATGCAAACGGAAATGTAACTTCTGTTCAAGGTGCATTTAACTTCAACTGTGACCCATACTACTCATTGCCAAAATGA", "aa": "MKSTIKNGKSFFGKTVPDYKAVTYLYERRDQEGVGAYNSDTRILDANGNVTSVQGAFNFNCDPYYSLPK", "aa_hexdigest": "39ee82c016e2bc1fcb674a55993c58f5", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7752.532500000003, "isoelectric_point": 8.75869083404541 }, "id": "GLJLFKCOHC_861", "locus": "GCA013368035_00853" }, { "type": "cds", "sequence": "contig_1", "start": 488674, "stop": 489006, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATAAAGAAAATGTTTACAAAGAAACAGAAAATACAATATCAGTTTCAAAACAAGAAGACGGAAAACTTATTGAAAGATCAGTTTATGGTTTAGGTTTTACCAAATTTGACTTAGAAGCTAAGGGTGTTGGATTCTCACAAGTTGGTGGCGACGAAACTGAAAGTGGAAAGACCATTTATGACCAAATCACAAAAATGGCAACCACATCAGAAGACACTCCAAACGATGTATATCAATCAGGATACAAAACATCAAAAGGTGTTTCAAAGAACATGACTGCTATTGCATCAGAAGTTGCTAAATTAATAGCAGGCGAAAATGGAGCATGA", "aa": "MYKENVYKETENTISVSKQEDGKLIERSVYGLGFTKFDLEAKGVGFSQVGGDETESGKTIYDQITKMATTSEDTPNDVYQSGYKTSKGVSKNMTAIASEVAKLIAGENGA", "aa_hexdigest": "c14f7e1eb6119632524ff80e19602fbc", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11907.04139999999, "isoelectric_point": 4.849070930480956 }, "id": "GLJLFKCOHC_862", "locus": "GCA013368035_00854" }, { "type": "tRNA", "sequence": "contig_1", "start": 490280, "stop": 490369, "strand": "-", "gene": "trnS", "product": "tRNA-Ser(cga)", "amino_acid": "Ser", "anti_codon": "cga", "score": 64.9, "nt": "GGATAGTTGCTCGAGAGGCTGAAGAGGTTAGTCTCGAAAACTAATAATGTTTTCTAACATTCAAGGGTTCAAATCCCTTACTATCCGCCA", "db_xrefs": [ "SO:0000269" ], "anti_codon_pos": [ 490356, 490358 ], "id": "GLJLFKCOHC_26", "locus": "GCA013368035_00855" }, { "type": "cds", "sequence": "contig_1", "start": 490629, "stop": 491915, "strand": "+", "frame": 3, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "db_xrefs": [ "COG:COG0696", "COG:G", "EC:5.4.2.12", "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537", "KEGG:K15633", "SO:0001217", "UniRef:UniRef50_Q8EW33", "UniRef:UniRef90_Q6Y8Q8" ], "nt": "ATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGTCTAAGAAAAGAAAGGCAAGGAAATGCTTATTTGCAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATACGTTGGTTTGCCAGAGGGACAAATTGGTAATAGCGAAGTAGGACATTTAAATATTGGTGCAGGTAGAGTTGTATATACGGGATTATCTTTAATTAATAAGGCAATTGAAAATAACACTTTTAAGGATAATGAAATTTTAAATGATGTCATTGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATCCTAAAATTAGCGCATAGCAAAGGATTAAAAAATGTAAGCGTTCATGTGTTTGGTGATGGAAGAGATGTAAAACCACAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGGTTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAAGCATATGATGCTATTTTAGGGCAATCAAAAAATGTTATTGACAACATTGATGATTACATTGAAAGTCAATATACAAAAGGAATATTTGATGAATTTTTTGAACCTGCCCAATTAAAAGATGGCTCATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGACAGAGCAAGACAGCTAAGCCATATGTTTATAGGTTCAAATTTATACACATACGAACCAAAAAACAAAGTACTAATCGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAGGTCGCATTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAGTAATGACATTAAACAATTAAGACTTGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGAGGGGTTGACATCGAATATAAAAATGAAAATAGAATTTTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCTAAAGAAATTACAGATTCACTATTGAACAACATTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGCCATACCGGTAATTTGAACGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTTCAAAGAATATTAGAATATGTATCATTAAATCATGAAAATGTAACCTGA", "aa": "MKKIILTIIDGLGLRKERQGNAYLQAKHPCFDYLFSMCPNSVLQASGQYVGLPEGQIGNSEVGHLNIGAGRVVYTGLSLINKAIENNTFKDNEILNDVIDKTIKNNTTLHVMGLLSNGGVHSLDLHLFEILKLAHSKGLKNVSVHVFGDGRDVKPQSIKNSLETLKDLCQKFGYKISSISGRFYAMDRDSIFSRNQEAYDAILGQSKNVIDNIDDYIESQYTKGIFDEFFEPAQLKDGSFVKNGDSIIFFNFRPDRARQLSHMFIGSNLYTYEPKNKVLIDNFVSLMKYEGINSKVAFKEMEVVNPLGKVLESNDIKQLRLAETQKYAHVTFFFDGGVDIEYKNENRILVDSLKVDSFADYPHMSAKEITDSLLNNIEKNDFIIMNYANPDMVGHTGNLNATIEAIEFLDSQFQRILEYVSLNHENVT", "aa_hexdigest": "f03242576157278be985704c878c5a55", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_Q6Y8Q8", "query_cov": 1.0, "subject_cov": 0.8577154308617234, "identity": 0.9840000000000001, "score": 835.0, "evalue": 1.95e-304, "valid": true, "gene": "gpmI", "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase", "ec_ids": [ "5.4.2.12" ], "uniref50_id": "UniRef50_Q8EW33", "cog_id": "COG0696", "cog_category": "G", "kegg_orthology_id": "K15633", "go_ids": [ "GO:0005829", "GO:0006007", "GO:0006096", "GO:0030145", "GO:0046537" ] }, "pscc": { "uniref50_id": "UniRef50_Q8EW33", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q8EW33" ], "product": "2,3-bisphosphoglycerate-independent phosphoglycerate mutase" }, "genes": [ "gpmI" ], "id": "GLJLFKCOHC_863", "locus": "GCA013368035_00856" }, { "type": "cds", "sequence": "contig_1", "start": 492000, "stop": 492128, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTATTTGTACAGATAAAAATGTTAATTTAGGAAACGGTAGTCTTTGTGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAATAAACCAAAAGAAATGACTGGAAAATCATTACTTAAATAA", "aa": "MFICTDKNVNLGNGSLCDVAPTILDYLKINKPKEMTGKSLLK", "aa_hexdigest": "f21036978f1a261326b2f7bd76b52c6c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4627.4694, "isoelectric_point": 8.70988826751709 }, "id": "GLJLFKCOHC_864", "locus": "GCA013368035_00857" }, { "type": "cds", "sequence": "contig_1", "start": 492169, "stop": 492657, "strand": "-", "frame": 2, "gene": null, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "nt": "TTGCAAGATACTTTTCTATTTAAAACAACAATTTTAGAAAATCTAAGATATGCTAATCCTAGTGCAAGCAATGAAGAAATTATTAATGCTGCCAAAATTACAAAGGCCGATAATTTTATAAGACAACTTGAACATGGTTACGAAGAAATAGTTGAAGAAGGCGGAAGCAATTTTTCACAAGGTGAAAGACAGATGCTTGCAATCACAAGAGCAATAATTGCTAATAAAAATATTTTGATATTAGATGAAGCAACATCTAATGTAGATACAAGAACAGAAAAGAATATTCAAGACGCAATGTTAGAGTTAATGAAAGGAAAAACTTCATTTGTTATTGCTCATAGACTTTCAACAATAGTAAATGCTAGCCAAATTTTAGTTGTTAAAGATGGAAAAATTATTGAACAAGGTACACACAAAGAACTATTGACCAAAGGTAGTTTTTATGAAAAGCTATATCATTCATCTTTTAGTGAAGAAAATGAATAA", "aa": "MQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE", "aa_hexdigest": "c4f9e9a000d7b0695385d06c1f095888", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0NZZ8", "query_cov": 0.9567901234567902, "subject_cov": 1.0, "identity": 0.735, "score": 229.0, "evalue": 5.29e-75, "valid": false, "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component", "uniref50_id": "UniRef50_A0A3B0NZZ8" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0NZZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0NZZ8" ], "product": "ABC-type multidrug/protein/lipid (MdlB-like) transport system component" }, "genes": [], "id": "GLJLFKCOHC_865", "locus": "GCA013368035_00858" }, { "type": "cds", "sequence": "contig_1", "start": 492679, "stop": 493740, "strand": "-", "frame": 2, "gene": "mdlB", "product": "ABC transporter ATP binding protein", "db_xrefs": [ "COG:COG1132", "COG:V", "SO:0001217", "UniRef:UniRef50_A0A448ZXF5", "UniRef:UniRef90_D1J8H0" ], "nt": "TTGATCTTAGGCTATATTTTAGAAAAAGTCTTTTCATTTATATGTTCAGCAATGGCAGTTAAGGCAGGAATTTATGGCTCAGAAATAATGCGGAGTAAGGCTTATAAATCAATAATGAAAATGCCAATCCAATTTTTTGATACTGTTAGCTCGGGAGAATTAATGTCAATATTGTCAAATGATATTGATAATGTTTCATATGGTATATGTGGAACCTTAAACTCGTTATTAGCAGCGATATTTAGTGCTTTAACATCGCTTGGATTTATGTTTTACTATTCTACATATTTAACACTTATATATATTGCCTTATTTCCTGTTTTGTTGATATTGGCTTTTGTTCTAATTAAAAAAGCAATTCCATTATTTGGTAAGACTCAAAGAAATATAGGTTTATTGAATGGTTTTGTTGAAGAAAATTTGGCAGCACACCAATTAATAAATTCATTGAATAGTCAACAAATTATTAACGAACAATTTGATTTATATAATGAAAAATTATATAAACCTTCATTAAAAGCAAATATATATGCTGGAATAGTTTATCCATATGGAAATGCAATAACATTCATAATGCAATTAATAATAATTTTTGTTGGCGGAGCATTTGCATTGTCTGGAATTGGCACTGGCTCAAATAGTAATTTTACAGTCGGTACATTGACATCATTTATTTTGTATATAAAAATTACTTCTAGTCAAATAATTAAAAATTTTGAAAATATTTCAATGATTCAAACAGCAACAATATCAACAACAAGAGTATTAAATTTAATTAGAATGCATCCGGCTATAGATGAAAGCAAACTTGCTACAATATCAAATGTGAATGGTGATATTGAATTTAAAAATGTAAGTTTTTCATATGATAATTCTTTAAACAATTTACAATTGAAAAATATTTCATTCAAAGCAAAAAAAGGTCAAGTGTTTGCTATTGTAGGACCTACCGGCGCTGGCAAAACTACAATAATAAATTTACTAAGCAAATTTTATTTGCCTATTGAAGGAGAAATAAAAATTGATGATTTTAAATCAAGCGAAATAAATGAATCTAGTTGA", "aa": "MILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVLIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAIDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDFKSSEINESS", "aa_hexdigest": "4ec08c4ee7d360e74a0106e010d86ca8", "start_type": "TTG", "rbs_motif": null, "genes": [ "mdlB" ], "truncated": "both", "pseudogene": { "start": 492376, "stop": 494040, "observations": { "mutation": [ 492681, 493791 ], "stop": [ 492681, 493791 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H0", "query_cov": 1.0, "subject_cov": 0.5648, "identity": 0.9890000000000001, "score": 652.0, "evalue": 2.52e-231, "gene_start": 1, "gene_end": 353, "reference_start": 103, "reference_end": 455, "reference_sequence": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKFWNSNSNGLFEFGIAIGILILGYILEKVFSFICSAMAVKAGIYGSEIMRSKAYKSIMKMPIQFFDTVSSGELMSILSNDIDNVSYGICGTLNSLLAAIFSALTSLGFMFYYSTYLTLIYIALFPVLLILAFVFIKKAIPLFGKTQRNIGLLNGFVEENLAAHQLINSLNSQQIINEQFDLYNEKLYKPSLKANIYAGIVYPYGNAITFIMQLIIIFVGGAFALSGIGTGSNSNFTVGTLTSFILYIKITSSQIIKNFENISMIQTATISTTRVLNLIRMHPAVDESKLATISNVNGDIEFKNVSFSYDNSLNNLQLKNISFKAKKGQVFAIVGPTGAGKTTIINLLSKFYLPIEGEIKIDDYKSSEINESSWRNQISIVLQDTFLFKTTILENLRYANPSASNEEIINAAKITKADNFIRQLEHGYEEIVEEGGSNFSQGERQMLAITRAIIANKNILILDEATSNVDTRTEKNIQDAMLELMKGKTSFVIAHRLSTIVNASQILVVKDGKIIEQGTHKELLTKGSFYEKLYHSSFSEENE" }, "paralog": false, "identity": 0.9909747292418772, "score": 1055.0, "evalue": 0.0, "description": "Internal stop codon at 492681, 493791. Nonsense mutation around 492681, 493791.", "psc": { "uniref90_id": "UniRef90_D1J8H0", "gene": "MdlB", "product": "ABC transporter ATP binding protein", "uniref50_id": "UniRef50_A0A448ZXF5", "cog_id": "COG1132", "cog_category": "V", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H0", "UniRef:UniRef50_A0A448ZXF5", "COG:COG1132", "COG:V" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXF5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXF5" ], "product": "ABC-type multidrug/protein/lipid transport system ATPase component" } }, "id": "GLJLFKCOHC_866", "locus": "GCA013368035_00859" }, { "type": "cds", "sequence": "contig_1", "start": 493789, "stop": 494046, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAGAGATATTCTTGATCCACTAAAAGTTGATATTGATTTAAAAAAAGTATCTAAATTTGAAAGAAAAGCATTTCGAAAAAAACAACACGCAATAAAATCGGAAAGTTTTAAAGAATTAATTAAATATTTAAATTACAAACGTGGATATGTTTTTTTAATAATAATAATTACTTTAATTTCTTCATTGTGTTTAACTGCTGCCACATACGGTTTGGGTTATATTATCGACCATTATTTAAACTTTAAGTTTTGA", "aa": "MNRDILDPLKVDIDLKKVSKFERKAFRKKQHAIKSESFKELIKYLNYKRGYVFLIIIITLISSLCLTAATYGLGYIIDHYLNFKF", "aa_hexdigest": "a80f3f94ab1456aec97f2b99dee99346", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10062.88029999999, "isoelectric_point": 9.714434623718262 }, "id": "GLJLFKCOHC_867", "locus": "GCA013368035_00860" }, { "type": "cds", "sequence": "contig_1", "start": 494039, "stop": 494941, "strand": "-", "frame": 1, "gene": null, "product": "Multidrug ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "nt": "ATGAATTATGTTATGACTGTTACATTTGGAGTTGTTGCTTCATCATTTACTACATTTAATATTTTCAAGGCAAATGTATCCTCAAAACGTATTTTTGAAATTATGAACAAAAAGCCAGATATTGCAAAAATCAAATCTGATAAGCTAATTGTTAACGGAGAAATTGAATTTAGCCATGTATCTTTTAAATATTATGAATCTGCCAAATCTAATGTTTTAGAAGATATTTCATTTAGTTTAAAGCCTGGTAAGGTTTTAGGCATAATTGGCCCTACTGGTTCTGGAAAATCAACTATTGCAAAATTGTTAAATTTAGATTTTAAAACTCAAGACGGGCTAGTAACAATTGATGGGCATAATATTCAAGAAATTGATACAGACTCATTGAGAAAGAATATCTCTCACGTTTATCAAAACCCTTGCTTATTAAGTGGCACAATAAAAAGCAATTTATTGCTTGCTAAACCTAATGCCACTGATGAAGAGATTGAATTAGCGGCAAAAAATGGTTGCGCTTTTGAATATATCAATAAGTTTAAAGAAAAATTTGAACATGTTGTTGAACAAAAGGGTGCAAATTTATCAGGCGGTCAAAAACAAAGATTATCTATTGCCCAAGGTTTAATTAAACGACCTAAAATATTGATTTTAGATGATTCTACTTCAGCATTGGATGCTAGAACAGAAGCTATGGTAAGAAACAATATTAAAGAAGAATTTAAATATGAAAAAATATCATTAGTTATTATTGCTCAAAAAATTTCAGCAATTATTGATGCAGATGAAATTTTAGTATTAAACCAAGGAAAAATTATTGATCGTGGCAATCATGAGGAATTAATTTCTAGAGATGGTCTATATAAAGAAATTGCATATTCACAGTTAGGGGGTAATAATGAATAG", "aa": "MNYVMTVTFGVVASSFTTFNIFKANVSSKRIFEIMNKKPDIAKIKSDKLIVNGEIEFSHVSFKYYESAKSNVLEDISFSLKPGKVLGIIGPTGSGKSTIAKLLNLDFKTQDGLVTIDGHNIQEIDTDSLRKNISHVYQNPCLLSGTIKSNLLLAKPNATDEEIELAAKNGCAFEYINKFKEKFEHVVEQKGANLSGGQKQRLSIAQGLIKRPKILILDDSTSALDARTEAMVRNNIKEEFKYEKISLVIIAQKISAIIDADEILVLNQGKIIDRGNHEELISRDGLYKEIAYSQLGGNNE", "aa_hexdigest": "b376c7e2146eb1cab9d1431a53a9542f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0031CC58F0", "query_cov": 0.8, "subject_cov": 0.9876543209876543, "identity": 0.5, "score": 212.0, "evalue": 9.32e-65, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_A0A3D3WBZ0" }, "pscc": { "uniref50_id": "UniRef50_A0A3D3WBZ0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D3WBZ0" ], "product": "Multidrug ABC transporter ATP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_868", "locus": "GCA013368035_00861" }, { "type": "cds", "sequence": "contig_1", "start": 495503, "stop": 495862, "strand": "-", "frame": 1, "gene": null, "product": "ABC transporter transmembrane domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8", "UniRef:UniRef90_UPI00241122C8" ], "nt": "ATGTTTAAACTTTATAAATTAATGGCCAACAAATTCAAATGTTTGTCATTTTTAACTATATCTTTAACATTGCTACAAGTAGTTTCATTTTTAGTATTGCCAATATTATTGGGACAATTGACAAGGTTAATTGGTGAAAATGCATATTTAATTCAAAATAATTTGTCAACAAGCAGACCAATAACAATTGAAATTCTAAGAATTAATTTTTTATGTCAAAGCCATCAGAGTGCGTTAATGCATTTGGGAGGATATTTTGCATTGTTTTTAATAATAGGAACTATTTCAGCAATGTGTGCTTCATTGCTTGCATCATATGTGTCTCAAGCAGGATCAAAACAAATAAGAAGTTGTTTATGA", "aa": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTSRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCL", "aa_hexdigest": "45845ab3c9d8d72670d301c333bd4ca4", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 495202, "stop": 495862, "observations": { "insertion": [ 495238 ], "mutation": [ 495253, 495505 ], "stop": [ 495253, 495505 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241122C8", "query_cov": 1.0, "subject_cov": 0.5265486725663717, "identity": 0.992, "score": 214.0, "evalue": 8.28e-69, "gene_start": 1, "gene_end": 119, "reference_start": 1, "reference_end": 119, "reference_sequence": "MFKLYKLMANKFKCLSFLTISLTLLQVVSFLVLPILLGQLTRLIGENAYLIQNNLSTNRPITIEILRINFLCQSHQSALMHLGGYFALFLIIGTISAMCASLLASYVSQAGSKQIRSCLWKHLGELSQKDIEAFSNAKILTRFTIDISRIQTGLMSFLRMLIIGPFNLVLGLVFALLTNLQLSMIFLVVIPLLTLTMVISGVIWNPIQKKNKKCMTKLTLKVEKIF" }, "paralog": false, "identity": 0.9863013698630136, "score": 403.0, "evalue": 1.13e-145, "description": "Internal stop codon at 495253, 495505. Frameshift due to insertion around 495238. Nonsense mutation around 495253, 495505.", "psc": { "uniref90_id": "UniRef90_UPI00241122C8", "product": "ABC transporter transmembrane domain-containing protein", "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241122C8", "UniRef:UniRef50_UPI00241122C8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241122C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241122C8" ], "product": "ABC transporter transmembrane domain-containing protein" } }, "id": "GLJLFKCOHC_869", "locus": "GCA013368035_00862" }, { "type": "cds", "sequence": "contig_1", "start": 495911, "stop": 496324, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAATGAAGAATCAGGATTATTGGGAGTTTCAAAAATTTCTTCTGATATTCGTGACGTTGAAGAAGCTAAAAAAACAAATGAAAGAGCAAAATTTGCAATAGATTTATATGTAAGAAAAATAGCAGATTATTTAATTAATTACATGAATAAGATTGAAGGAAAAATCGATGCTATTGTTTTTACGGCTGGTGTGGGTGAAAATGATGAATATATTCGCCAAGCAGTTATTGATAAAATTCATTTAGTTAAATTAGAAATTGATAGTGAACTAAATAAAGGAAGAGATTTTGATTCATACAAATTAATTTCAACTAAAAATTCTTCAATTCCTATTTATATAATAAGGGCAGATGAAGAACTTTATATTGCAAAAGAAGTTAAACAATTTTTTGATGATAATATAAGGTAA", "aa": "MLNEESGLLGVSKISSDIRDVEEAKKTNERAKFAIDLYVRKIADYLINYMNKIEGKIDAIVFTAGVGENDEYIRQAVIDKIHLVKLEIDSELNKGRDFDSYKLISTKNSSIPIYIIRADEELYIAKEVKQFFDDNIR", "aa_hexdigest": "772ca0d4573f13561032f57be5643bcb", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15767.787399999987, "isoelectric_point": 5.012255668640137 }, "id": "GLJLFKCOHC_870", "locus": "GCA013368035_00863" }, { "type": "cds", "sequence": "contig_1", "start": 496346, "stop": 496690, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACATTCCACTTTGATACTTCATTCCATAAAACCCTTCCAAAAGAAGCATATACTTATGCAATTGATGCAAATATAACCGATGAATTAAATGTTAGAAAATATGGTTTCCACGGCTTGAATCACCACTTTATTACATTAAAATTGCAAGAAATTTTAAATAAAGACAAAGTTAATTTTGTTAATTTACATATCGGTAATGGTGCAAGTTTAGCAGCCATTCAAGATAGTAAATCAATTGATACCTCAATGGGATTTACACCACTAGCGGGAGTTATGATGGGCACTCGTTCTGGTGATGTTGATTGTTCAATTATTCCATATATTATGAAACATAAAAATTGA", "aa": "MTFHFDTSFHKTLPKEAYTYAIDANITDELNVRKYGFHGLNHHFITLKLQEILNKDKVNFVNLHIGNGASLAAIQDSKSIDTSMGFTPLAGVMMGTRSGDVDCSIIPYIMKHKN", "aa_hexdigest": "bf9ea4f0f79cb2cb311df2ef9af91b04", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12773.492099999985, "isoelectric_point": 7.055731010437011 }, "id": "GLJLFKCOHC_871", "locus": "GCA013368035_00864" }, { "type": "rRNA", "sequence": "contig_1", "start": 497195, "stop": 497299, "strand": "-", "gene": "rrf", "product": "5S ribosomal RNA", "coverage": 0.8823529411764706, "score": 60.7, "evalue": 1.2e-09, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00001", "KEGG:K01985", "SO:0000652" ], "nt": "CGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTT", "id": "GLJLFKCOHC_38", "locus": "GCA013368035_00865" }, { "type": "cds", "sequence": "contig_1", "start": 497402, "stop": 497599, "strand": "-", "frame": 1, "gene": null, "product": "Smr domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26", "UniRef:UniRef90_D1J8H3" ], "nt": "ATGAAGTTCAATTTTTTTCCAATGGTGTTAGATCTTCATGGTTATGATGTAAACTCTGCAACAGCAGCAATTTTAAACGCTTTGTATGAATTTAAACAAGATGAATATAATAATTACTTCGATATTATTGTAGGCATTGGTAGTGGGATTATTCGTCAAATAACGGAAGATTTATTAGACAAAGAAGGATATTATTGA", "aa": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFKQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYY", "aa_hexdigest": "ea0ebfb1e6a408a77567f0648b2a77c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 497354, "stop": 497599, "observations": { "mutation": [ 497404 ], "stop": [ 497404 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H3", "query_cov": 1.0, "subject_cov": 0.7926829268292683, "identity": 0.985, "score": 135.0, "evalue": 2.25e-40, "gene_start": 1, "gene_end": 65, "reference_start": 1, "reference_end": 65, "reference_sequence": "MKFNFFPMVLDLHGYDVNSATAAILNALYEFEQDEYNNYFDIIVGIGSGIIRQITEDLLDKEGYYWKNIGNSLAKIRVFKKI" }, "paralog": false, "identity": 0.9629629629629629, "score": 159.0, "evalue": 1.68e-52, "description": "Internal stop codon at 497404. Nonsense mutation around 497404.", "psc": { "uniref90_id": "UniRef90_D1J8H3", "product": "Smr domain-containing protein", "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H3", "UniRef:UniRef50_B3PN26" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN26" ], "product": "Smr domain-containing protein" } }, "id": "GLJLFKCOHC_872", "locus": "GCA013368035_00866" }, { "type": "cds", "sequence": "contig_1", "start": 497600, "stop": 498463, "strand": "-", "frame": 1, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "db_xrefs": [ "EC:3.2.2.23", "EC:4.2.99.18", "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872", "KEGG:K10563", "SO:0001217", "UniRef:UniRef50_A0A9Q9F2U2", "UniRef:UniRef90_A0A454C9Y4" ], "nt": "ATGAGTAATGTCATTCGAAAGTATTATAATTTTACTATGCCTGAATTACCTGAAGTTAGGGTTGTTGTTAAAGCCCTTAATAATACTATATTAAATAAAAAAATAACAAATTTGATTATATACAAACCCAAAATTTTTAAAGAGTTTAGCCCTCAATATTTCATTTCAATTTTAAGAGATAAAAAAATTGAAAAAATAGATAACATTGGCAAACATATAATTTTCTTTTTATCAGAAAAATTAGTTTTACTTTCTCATTTAAGAATGGAAGGGAAATATCGTTATTATTTAAACCCTCCAAAAGAAATTGATAAGCACTTAGTTGCTAGATTTGTTTTTAGCGACAATAGCGAATTGCACTTTTTAGATAAACGTTTATTTGGCACATATATGCTAAGAAACTTAGAAAATTATAACAAAATTCCTCCAATTTCTTTGCTTGGACTTGAACCAAAAGATATTGACATTGAAGCATTATTTAAAAAAATAAAAAATTCAAAAATGCCCATTAAAACAAAATTACTTGATCAGAGTTTTGTTGCTGGTATAGGAAATATTTACGCTGATGAAGCATTGTTTGCTTCCAAAATTCATCCTCTTTCAAAAGCTTCTGATTTATCACTAGAGCAACTTAAAGACATAATAAAAAATGCAAATGAAATAATGGAAAAAAGTTATGAATTAGGTGGAACAACGTTATTTTCTTATGAATCATTAAATAAACAAGAAGGAAAATATCAAGACTTTTTAAATATTCATAGTGATTCTATAAAAAAATGTAAGGTTTGTGGCCAAAAAACACTTAAATTAAAAGTTAACCAACGTGGTACATATGTTTGCCCTAATTGTCAGAAGGTATATTAA", "aa": "MSNVIRKYYNFTMPELPEVRVVVKALNNTILNKKITNLIIYKPKIFKEFSPQYFISILRDKKIEKIDNIGKHIIFFLSEKLVLLSHLRMEGKYRYYLNPPKEIDKHLVARFVFSDNSELHFLDKRLFGTYMLRNLENYNKIPPISLLGLEPKDIDIEALFKKIKNSKMPIKTKLLDQSFVAGIGNIYADEALFASKIHPLSKASDLSLEQLKDIIKNANEIMEKSYELGGTTLFSYESLNKQEGKYQDFLNIHSDSIKKCKVCGQKTLKLKVNQRGTYVCPNCQKVY", "aa_hexdigest": "9699d42eca500bcfa77f56fe83d2272b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454C9Y4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.993, "score": 566.0, "evalue": 7.94e-204, "valid": true, "gene": "mutM", "product": "DNA-formamidopyrimidine glycosylase", "ec_ids": [ "3.2.2.23", "4.2.99.18" ], "uniref50_id": "UniRef50_A0A9Q9F2U2", "kegg_orthology_id": "K10563", "go_ids": [ "GO:0005737", "GO:0006281", "GO:0008534", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A9Q9F2U2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A9Q9F2U2" ], "product": "DNA-(apurinic or apyrimidinic site) lyase" }, "genes": [ "mutM" ], "id": "GLJLFKCOHC_873", "locus": "GCA013368035_00867" }, { "type": "cds", "sequence": "contig_1", "start": 498505, "stop": 498681, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGCGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGA", "aa": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKE", "aa_hexdigest": "fffbfe4be100210b6dc72de2222d76bc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6619.7276, "isoelectric_point": 9.629852104187012 }, "id": "GLJLFKCOHC_874", "locus": "GCA013368035_00868" }, { "type": "cds", "sequence": "contig_1", "start": 498760, "stop": 498972, "strand": "+", "frame": 1, "gene": "tsaC", "product": "L-threonylcarbamoyladenylate synthase", "db_xrefs": [ "COG:COG0009", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A077L684", "UniRef:UniRef90_D1J8H5" ], "nt": "ATGCCAAACCAAAAGAAATTGCTTGAATATATAGAAGTAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAATGCACCTGTATGTAAAACTATTGAAAATGCAAAGCAATTATTCCCCGAAGTTACTAATGTATATAATTTTGGTCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAA", "aa": "MPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK", "aa_hexdigest": "31e42a5462e277f60c3012de9ffa43e2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaC" ], "truncated": "5-prime", "pseudogene": { "start": 498505, "stop": 498972, "observations": { "mutation": [ 498679 ], "stop": [ 498679 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8H5", "query_cov": 1.0, "subject_cov": 0.45161290322580644, "identity": 1.0, "score": 147.0, "evalue": 6.82e-44, "gene_start": 1, "gene_end": 70, "reference_start": 86, "reference_end": 155, "reference_sequence": "MINKYQKLIISTTDTVLGIGGPVSKEVEDLIYEIKGRDRNKKLIILVSSIKQARQFKEWNSQADEFAKKYWPGATTIVVNNQGFRMPNQKKLLEYIEVNGPIYMTSCNLSNAPVCKTIENAKQLFPEVTNVYNFGPMTQKPSQIIRVEDGEILRK" }, "paralog": false, "identity": 0.9935483870967742, "score": 308.0, "evalue": 7.97e-110, "description": "Internal stop codon at 498679. Nonsense mutation around 498679.", "psc": { "uniref90_id": "UniRef90_D1J8H5", "gene": "TsaC", "product": "L-threonylcarbamoyladenylate synthase", "uniref50_id": "UniRef50_A0A077L684", "cog_id": "COG0009", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8H5", "UniRef:UniRef50_A0A077L684", "COG:COG0009", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L684", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L684" ], "product": "L-threonylcarbamoyladenylate synthase" } }, "id": "GLJLFKCOHC_875", "locus": "GCA013368035_00869" }, { "type": "cds", "sequence": "contig_1", "start": 498974, "stop": 499369, "strand": "+", "frame": 2, "gene": "rpiB", "product": "Ribose-5-phosphate isomerase", "db_xrefs": [ "COG:COG0698", "COG:G", "SO:0001217", "UniRef:UniRef50_D1J8H6", "UniRef:UniRef90_D1J8H6" ], "nt": "ATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGACGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCAGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGTGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAGTTGAAAAAATTTTTCATAGCTGA", "aa": "MKTTVKITSDHAGYDAKINLAKRLENEGYNVELFGSKTNNNSDSYADAAIELVNEIHKDGNPDENKNVKYVAFCGSGIGIEMTLNKFKGIRAARVTNENESHLAKLHNNANVLCMGGRLLEGNEVEKIFHS", "aa_hexdigest": "56578a33b1ae849663dc0a78aefbb2c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8H6", "query_cov": 1.0, "subject_cov": 0.8451612903225807, "identity": 1.0, "score": 265.0, "evalue": 2.05e-89, "valid": true, "gene": "RpiB", "product": "Ribose-5-phosphate isomerase", "uniref50_id": "UniRef50_D1J8H6", "cog_id": "COG0698", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_D1J8H6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8H6" ], "product": "Ribose-5-phosphate isomerase" }, "genes": [ "rpiB" ], "id": "GLJLFKCOHC_876", "locus": "GCA013368035_00870" }, { "type": "cds", "sequence": "contig_1", "start": 499473, "stop": 499664, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTAGAAGTATTAAATCTTCCAGATACGTTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCGGCAGCCAAAATTTCTTTCTTAATCATATTTTGTTCCAAGAACAAACAATATGTTTCAATTTTAACCGTTTCTGTATTGGTATAA", "aa": "MSLFLFLYIFYFSILEVLNLPDTFSTFDEVIHALLSAAKISFLIIFCSKNKQYVSILTVSVLV", "aa_hexdigest": "6c0c4c9537bfc19bcab723c3f5889836", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7227.5594, "isoelectric_point": 5.4644666671752935 }, "id": "GLJLFKCOHC_877", "locus": "GCA013368035_00871" }, { "type": "cds", "sequence": "contig_1", "start": 500498, "stop": 500686, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6", "UniRef:UniRef90_UPI00036493F6" ], "nt": "ATGGTCATGATTATTATTTCAGCCGTTTTATACAATATTGGTGTTGCAACATTTTTGGCTAAGGCTGCCACTGTTGCAACTGGGTTATCTGCATTGGTTCAAGCCATTACTCTAACATTAACTCAAACTGCACCATATTTTGCTTATATTTATATAGCAATGAATTTACCTTTTGTAATTGCATTTTGA", "aa": "MVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAF", "aa_hexdigest": "e9ecd32b7bb53b225037a9f72ff45509", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 500467, "stop": 500836, "observations": { "insertion": [ 500494 ], "mutation": [ 500500, 500707 ], "stop": [ 500475, 500500, 500707 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00036493F6", "query_cov": 1.0, "subject_cov": 0.5081967213114754, "identity": 1.0, "score": 103.0, "evalue": 2.01e-27, "gene_start": 1, "gene_end": 62, "reference_start": 51, "reference_end": 112, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKMVMIIISAVLYNIGVATFLAKAATVATGLSALVQAITLTLTQTAPYFAYIYIAMNLPFVIAFWKKILDYLWY" }, "paralog": false, "identity": 0.9754098360655737, "score": 221.0, "evalue": 3.12e-76, "description": "Internal stop codon at 500475, 500500, 500707. Frameshift due to insertion around 500494. Nonsense mutation around 500500, 500707.", "psc": { "uniref90_id": "UniRef90_UPI00036493F6", "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00036493F6", "UniRef:UniRef50_UPI00036493F6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00036493F6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00036493F6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6600.926899999999, "isoelectric_point": 8.138311576843265 }, "id": "GLJLFKCOHC_878", "locus": "GCA013368035_00872" }, { "type": "cds", "sequence": "contig_1", "start": 500705, "stop": 500836, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B", "UniRef:UniRef90_UPI002410BF5B" ], "nt": "ATGAAACATACAGCTGAGGAACTTGCTGAGATTAAAGAACGTAAACGTTTAAAGAAAGAAACCAAAAAAGAAAAGGGATCAGTCGGCAAAATTGACATAAATCCTTATGGGGTAAATTTCTTTAATATTTGA", "aa": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNI", "aa_hexdigest": "3bbac7b9d353dab09e5c0c372e3c1fba", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 500625, "stop": 500836, "observations": { "deletion": [ 500689 ], "mutation": [ 500707 ], "stop": [ 500707 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410BF5B", "query_cov": 1.0, "subject_cov": 0.6142857142857143, "identity": 1.0, "score": 84.0, "evalue": 1.76e-20, "gene_start": 1, "gene_end": 43, "reference_start": 1, "reference_end": 43, "reference_sequence": "MKHTAEELAEIKERKRLKKETKKEKGSVGKIDINPYGVNFFNIWKKFPKKNGHDYYFSRFIQYWCCNILG" }, "paralog": false, "identity": 0.9714285714285714, "score": 141.0, "evalue": 7.47e-46, "description": "Internal stop codon at 500707. Frameshift due to deletion around 500689. Nonsense mutation around 500707.", "psc": { "uniref90_id": "UniRef90_UPI002410BF5B", "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410BF5B", "UniRef:UniRef50_UPI002410BF5B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410BF5B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410BF5B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 5018.7903000000015, "isoelectric_point": 9.630819129943848 }, "id": "GLJLFKCOHC_879", "locus": "GCA013368035_00873" }, { "type": "cds", "sequence": "contig_1", "start": 501003, "stop": 501272, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCACTGCAATTTACTTATTAGTATTTTCAATGTTAGTAAATAAGTTCTATCCAAAATACAAGAAAGTAAGAATTGATATTTACACCACAAAACCAGAACTAGTTGCTGAAAGATTCAAACAAATTGGATATGTTCACTCATTTAATGTATTCAACGGATTTGGTGGATATAACCATCAAGATTTTGGAAAAATTGAAACAATTGCCCTATATTTAGAAAAAGAATCAATTTTAGAAGAAGTTAGAAGCGTTGATAAAAAAGCCTGA", "aa": "MGTAIYLLVFSMLVNKFYPKYKKVRIDIYTTKPELVAERFKQIGYVHSFNVFNGFGGYNHQDFGKIETIALYLEKESILEEVRSVDKKA", "aa_hexdigest": "5fb240ebea4d8a7c69636658fba4330d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10389.952999999994, "isoelectric_point": 9.12616062164307 }, "id": "GLJLFKCOHC_880", "locus": "GCA013368035_00874" }, { "type": "cds", "sequence": "contig_1", "start": 501534, "stop": 501740, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAGAGATGATTTAGCTATTGCTAAGTATCTAGCCAACTTATTTAAAACTAATGATTTAAAAATGGTATTTGATAATGCAAAATTAATTTTTGAAAAATCTAATTCTTTATCACTTGCTAATATTCCTAGTCTATTAACAGGTATTGATTTAAAAGGCGGTCAAAATACTAAAATTTATGGAATATATAATGGCCAAAATTGA", "aa": "MSRDDLAIAKYLANLFKTNDLKMVFDNAKLIFEKSNSLSLANIPSLLTGIDLKGGQNTKIYGIYNGQN", "aa_hexdigest": "169108d65567dd34dd6f21978959979e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7524.582299999997, "isoelectric_point": 9.049507713317869 }, "id": "GLJLFKCOHC_881", "locus": "GCA013368035_00875" }, { "type": "cds", "sequence": "contig_1", "start": 502050, "stop": 502229, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGTAAGTGCATTTTTATATAATATTGCAATTACAGTATTTTTAAAAAAAGCAGCAACAATAGCTTCAGGAACTTCATCTTTATCTCAAATAATTACTTTTACAGCGCCTGCAACTGCACAATTTTATGGTTTATTTTATGTATTAGTTAACCTACCTTTAATGTTTATTTATTGA", "aa": "MLVSAFLYNIAITVFLKKAATIASGTSSLSQIITFTAPATAQFYGLFYVLVNLPLMFIY", "aa_hexdigest": "1bf202222e35b1ddb3f6d176f53dd0d2", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6456.647599999998, "isoelectric_point": 9.303577613830566 }, "id": "GLJLFKCOHC_882", "locus": "GCA013368035_00876" }, { "type": "cds", "sequence": "contig_1", "start": 502257, "stop": 502385, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTTTAGAAGATAAAAAAATAACTAAAATCAATAAAATCATTCTTACCAAAGAGCAAAAACACCAACGGTATTTAGAAAAAATGAAAATTCTAAATCCACAAAAAATAAATTTAATAAATGTCTGA", "aa": "MSLEDKKITKINKIILTKEQKHQRYLEKMKILNPQKINLINV", "aa_hexdigest": "1873dbb81ad7f096745ecd14bcf5105a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5077.1061, "isoelectric_point": 9.998482322692869 }, "id": "GLJLFKCOHC_883", "locus": "GCA013368035_00877" }, { "type": "cds", "sequence": "contig_1", "start": 502425, "stop": 502958, "strand": "-", "frame": 3, "gene": null, "product": "Replicative DNA helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "nt": "ATGTATTTAAATGATAAACCTACATCAACAATTTCGGATATAACAACCATTGCAAAACGATTTGCTAGAAACAATAAAGTTGATCTAGTTGTAGTTGATTATCTACAACTAATGTCAGATTCTTCAAAAGCAAGTATGGAAAATCGTCAATTGGAAGTAAGCAAGATTTCGAGATCCTTAAAGCAACTTGCTAGAGAATTAGAATGTCCTGTTTTAGCATTAAGCCAGTTAAGTAGAAATGTTGAAAAACGGGAAGATAAAACTCCTATTTTGTCAGATTTAAGAGAATCAGGAACAATTGAACAAGATGCCGATATGGTTATTTTTTTACATAGAAAAGATTATTATAATAAGCACAACATTAATGATGACAATGATAATGCAGAAGCTGTTGACAAAAGTGAAATTGTCAATGGCAGTTCTTCTTTAACCAATGTCATTGTAGCAAAAAATAGACATGGGGCTATTGGAATTGTACAACTATTATTCCAACCAGAATGCAACAGGTTTATTTATGAAAAAAAGAGAAATTAA", "aa": "MYLNDKPTSTISDITTIAKRFARNNKVDLVVVDYLQLMSDSSKASMENRQLEVSKISRSLKQLARELECPVLALSQLSRNVEKREDKTPILSDLRESGTIEQDADMVIFLHRKDYYNKHNINDDNDNAEAVDKSEIVNGSSSLTNVIVAKNRHGAIGIVQLLFQPECNRFIYEKKRN", "aa_hexdigest": "7a8c75a3660358dff4bfd3e0c4db383c", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P211", "query_cov": 0.9322033898305084, "subject_cov": 0.9116022099447514, "identity": 0.703, "score": 218.0, "evalue": 1.17e-69, "valid": false, "product": "Replicative DNA helicase", "ec_ids": [ "5.6.2.3" ], "uniref50_id": "UniRef50_A0A449A6S1", "kegg_orthology_id": "K02314" }, "pscc": { "uniref50_id": "UniRef50_A0A449A6S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449A6S1" ], "product": "Replicative DNA helicase" }, "genes": [], "id": "GLJLFKCOHC_884", "locus": "GCA013368035_00878" }, { "type": "cds", "sequence": "contig_1", "start": 502986, "stop": 503429, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03796.21" ], "nt": "ATGGAAAACAAGCCTTATGAAATTTCAGATTTATTATCCAATTTACAATTGAATTTAATCAACCTAGATATTTCAGAAATAAATACTGCTTATGAACAAGTAGGAAATAAAGCAGATGAACTATTTAATAGAATAATGAGCAAAGAAAAATCAGATATTGGCGTTGGCCTTTCTTTTGGATTTCCACCATTGGATGAATTAATTTTAGGCGCCAATCCCGGAGATTTGATAATTTTAGCGGCTCGTCCAGCTATGGGAAAAACTGCTTTTGCTTTAAATATAGCAAATAATGTTGCCAAAAACAATAAAACGGTGCTATTTTTTTCTCTTGAAATGTCAAATTCACAAATTGTTCAAAGAATGATGTCAATTGATTCCTTGGTTCCGATTAGTTTAATGAGAAAAAATGAAATCTCAACAGAAAATGCTAAGAATCTAGCCTGA", "aa": "MENKPYEISDLLSNLQLNLINLDISEINTAYEQVGNKADELFNRIMSKEKSDIGVGLSFGFPPLDELILGANPGDLIILAARPAMGKTAFALNIANNVAKNNKTVLFFSLEMSNSQIVQRMMSIDSLVPISLMRKNEISTENAKNLA", "aa_hexdigest": "5c5f2f98c12d853157e1609e02d6be83", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03796.21", "name": "DnaB_C", "length": 92, "aa_cov": 0.6190476190476191, "hmm_cov": 1.0, "evalue": 7.566549571992158e-30, "score": 99.5001449584961, "start": 56, "stop": 146 } ], "seq_stats": { "molecular_weight": 16173.475199999992, "isoelectric_point": 4.827301597595214 }, "id": "GLJLFKCOHC_885", "locus": "GCA013368035_00879" }, { "type": "cds", "sequence": "contig_1", "start": 503541, "stop": 503822, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00772.26" ], "nt": "ATGAACATTCAAGAGGAAAGAGAATTAGCAATTATTAATAATGAAACATCGTTATTAGGTTTATTAATTAGGGAACCAAATTGTTATTTAACAATTGCTGAAATTTTAAAACCTGAAATGTTTCATTTTAAGAATAATCAAATTCTTTTTAAATCTATTTCAGAAGTTCATATTGCCTCTAAACAATTCGATATAGCCAACTTATTGAGTTATTTAAATGACCAAAAATTAATTGATCACATCGGTTCGTTTGGCTATAATGGTAGTGAATATATTAGCTGA", "aa": "MNIQEERELAIINNETSLLGLLIREPNCYLTIAEILKPEMFHFKNNQILFKSISEVHIASKQFDIANLLSYLNDQKLIDHIGSFGYNGSEYIS", "aa_hexdigest": "de7f77af7d54a33655db8caec9ccc4c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00772.26", "name": "DnaB", "length": 75, "aa_cov": 0.8064516129032258, "hmm_cov": 1.0, "evalue": 8.503845452875443e-16, "score": 53.34480285644531, "start": 12, "stop": 86 } ], "seq_stats": { "molecular_weight": 10717.135599999989, "isoelectric_point": 5.0043550491333 }, "id": "GLJLFKCOHC_886", "locus": "GCA013368035_00880" }, { "type": "cds", "sequence": "contig_1", "start": 503850, "stop": 504290, "strand": "-", "frame": 3, "gene": "rplI", "product": "50S ribosomal protein L9", "db_xrefs": [ "COG:COG0359", "COG:J", "GO:0003735", "GO:0005840", "GO:0006412", "RefSeq:WP_049342443.1", "SO:0001217", "UniParc:UPI00066D1476", "UniRef:UniRef100_UPI00066D1476", "UniRef:UniRef50_A0A318U7T8", "UniRef:UniRef90_D1J8I0" ], "nt": "ATGAAAGTAATATTAATTAAAGAATACCAAAAACATAAAAAAAATGAAATCATTGAAGTTACAGATGGTTTTGCAAAAAACTTTTTAATTAAAAATGGTTATGCTCAACCAATTAACAAACAAACATTGGCAAATTTAGATAGAGTTAAATCAAATATTGCAGAAGACTTAGCAAATCAAATAGCTAAAGCTAATGAAATCAAACAATTAATTGAATCATTAGTTTTAAAATTCTATTTAAAATCTAATGGAAATATAGTTCACGGAAACGTTACAACAAAAGCAATTGAAAAAGAATTAATAAAAAACAACATTAAAATCCCAAGCCATGCATTTGAATCAGGGGTAACATATAATACTTTTGGGTTGCATGATGTAAAAATTAAATTACATGACCAAGTAACTGCAATACTAAAAATAAATATCATAGAAGAGAAATAA", "aa": "MKVILIKEYQKHKKNEIIEVTDGFAKNFLIKNGYAQPINKQTLANLDRVKSNIAEDLANQIAKANEIKQLIESLVLKFYLKSNGNIVHGNVTTKAIEKELIKNNIKIPSHAFESGVTYNTFGLHDVKIKLHDQVTAILKINIIEEK", "aa_hexdigest": "ff0e7924a59e13854189cca27fbf2506", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00066D1476", "ncbi_nrp_id": "WP_049342443.1", "uniref100_id": "UniRef100_UPI00066D1476", "db_xrefs": [ "SO:0001217", "UniParc:UPI00066D1476", "RefSeq:WP_049342443.1", "UniRef:UniRef100_UPI00066D1476" ] }, "ips": { "uniref100_id": "UniRef100_UPI00066D1476", "uniref90_id": "UniRef90_D1J8I0" }, "psc": { "uniref90_id": "UniRef90_D1J8I0", "gene": "rplI", "product": "50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A318U7T8", "cog_id": "COG0359", "cog_category": "J", "go_ids": [ "GO:0003735", "GO:0005840", "GO:0006412" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318U7T8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U7T8" ], "product": "Large ribosomal subunit protein bL9" }, "genes": [ "rplI" ], "id": "GLJLFKCOHC_887", "locus": "GCA013368035_00881" }, { "type": "cds", "sequence": "contig_1", "start": 504271, "stop": 504594, "strand": "-", "frame": 2, "gene": null, "product": "DHHA1 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "nt": "TTGCAAGAAATTAAAAAAGGATATTTCTTGGCATACAAAGATATTGAAGCATCAACAGATATTATTTCAATAGCGTCAGATGAAATATTAAGAGTTAATGGTAGGAAAGCTGCATTTGTTGTTGCTAAATTACCAGGAACAAAAACATACAAAATGTCAGCAAGAGGAATTAATACAAATGTTCAAATCATAGCTGAAGCAGTTAACGGCGGTGGCCATTTTGGGGCAGCCGCTGCTGAAACAACTGAAAATTTGAATTTATTTGTTGATAATATAATACAAGCAATAGTGAGTGTGAAAGATAATGAAAGTAATATTAATTAA", "aa": "MQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN", "aa_hexdigest": "203427b7f42c4d179281510b7caf3d55", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P200", "query_cov": 1.0, "subject_cov": 1.009433962264151, "identity": 0.757, "score": 152.0, "evalue": 7.27e-46, "valid": false, "product": "Putative bifunctional signaling protein/50S ribosomal protein L9", "uniref50_id": "UniRef50_A0A432F9M3" }, "pscc": { "uniref50_id": "UniRef50_A0A432F9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9M3" ], "product": "DHHA1 domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_888", "locus": "GCA013368035_00882" }, { "type": "cds", "sequence": "contig_1", "start": 504676, "stop": 505839, "strand": "-", "frame": 2, "gene": "gdpP", "product": "GGDEF domain-containing protein", "db_xrefs": [ "COG:COG3887", "COG:T", "EC:3.1.4.59", "KEGG:K22927", "SO:0001217", "UniRef:UniRef50_A0A448ZXC6", "UniRef:UniRef90_D1J8I1" ], "nt": "ATGTATGAAATAGACGGATGCAATTACGAAGTTCATATATCGTTTGAAAAAAATCTAGTTATTTTAAGAGATGTTACTGCTCAACAAAATTTATTAGATGACTATAAAAAACAAAGAATTGTGTTTGGGGAATTGGACATTGACAATATTAATTTATATCAAACATTTTTATCCCAAGAAGAAATTTTTAAAATTTATGCTTCTGTAGTAAATTTATTAGATGAAATATCTAAAAAATACAATGTTATTTATAGACAATACGAAAATGGAAGATTCTTTTTAATAACAAATAAAGAAACATTAGAAGAACTAGAACGAGATAGTTTTAATGTTATAACTTCAGTAAAAAACAAAATAATTGTTAAAGATGTTTTAATATCAATTTCTGGTGGATTTGGTTATGGTATTTTTAAATACGATACGTTAGTCAAATTAGCAAAAGATGCTCTAATGCAATCTCAAATGCGGGGTGGCGATCAAATTACAGTTTTAACAAAAAATGAAAAAGCTAGACACTATGGCTCAAGCTCGGAAATTGATGTAAATCTATCAAGGACAAATGTAAACTATATAGCACAAAACTTATTAGAAAAGATAAAATCAAAAGATATTGAAAATGTTATAGCCTATGGCCATAAGAATGCAGACCTTGATGCTTTGGGTTCAACATATGCAATTTTGCAATTAGCATTAGCCTATAAAAAGAATGCTTATATTCAAAACAAGACATTTGATAGTACTACTCAAAGAGTTATTGATAAAATGTCCAAGGAAACTCAAGAATATTTTATTAGTCCTAAAGAAGCAGAAAGCATCAACAACGAAAAAACATTGGTTGTAATTTGCGATACTTCAGATGATACTAGAATTGAAAATGCTAATGTCTTTAAAAACATAAAAAAAGAAAATATCATGATAATTGATCATCATAGAATAAATAAAAATACTGATGAAATAGCATATAGAGAAAATGTATATATTGATTCCTCGGCATCATCTGCTTCTGAAATATTAACAGAAATCATCACATTGTTACATGAAGAAGATAAAATTAATAATGATACGGCGCAATATTTATTAGATGGAATTTATTTAGATACTAATACATTTAAAAAACAAACAAGTGCTAAAACTTTTGCAGCAGCATCAATATTGCAAAAATGA", "aa": "MYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSVKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANVFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQK", "aa_hexdigest": "a54399d0a8b0f5dde185ef0628650fa8", "start_type": "ATG", "rbs_motif": null, "genes": [ "gdpP" ], "truncated": "both", "pseudogene": { "start": 504373, "stop": 506139, "observations": { "mutation": [ 504678, 505863, 505944 ], "stop": [ 504678, 505863, 505944 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I1", "query_cov": 1.0, "subject_cov": 0.589041095890411, "identity": 0.995, "score": 741.0, "evalue": 1.17e-265, "gene_start": 1, "gene_end": 387, "reference_start": 136, "reference_end": 522, "reference_sequence": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKIIWASESLKIKFHNNLINKNIKDIFNIKQWTSDNQEFMYEIDGCNYEVHISFEKNLVILRDVTAQQNLLDDYKKQRIVFGELDIDNINLYQTFLSQEEIFKIYASVVNLLDEISKKYNVIYRQYENGRFFLITNKETLEELERDSFNVITSIKNKIIVKDVLISISGGFGYGIFKYDTLVKLAKDALMQSQMRGGDQITVLTKNEKARHYGSSSEIDVNLSRTNVNYIAQNLLEKIKSKDIENVIAYGHKNADLDALGSTYAILQLALAYKKNAYIQNKTFDSTTQRVIDKMSKETQEYFISPKEAESINNEKTLVVICDTSDDTRIENANAFKNIKKENIMIIDHHRINKNTDEIAYRENVYIDSSASSASEILTEIITLLHEEDKINNDTAQYLLDGIYLDTNTFKKQTSAKTFAAASILQKWGAQNDKSVDTLKFDEKVFKIVQELLANLQEIKKGYFLAYKDIEASTDIISIASDEILRVNGRKAAFVVAKLPGTKTYKMSARGINTNVQIIAEAVNGGGHFGAAAAETTENLNLFVDNIIQAIVSVKDNESNIN" }, "paralog": false, "identity": 0.9914965986394558, "score": 1123.0, "evalue": 0.0, "description": "Internal stop codon at 504678, 505863, 505944. Nonsense mutation around 504678, 505863, 505944.", "psc": { "uniref90_id": "UniRef90_D1J8I1", "gene": "GdpP", "product": "GGDEF domain-containing protein", "ec_ids": [ "3.1.4.59" ], "uniref50_id": "UniRef50_A0A448ZXC6", "cog_id": "COG3887", "cog_category": "T", "kegg_orthology_id": "K22927", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I1", "EC:3.1.4.59", "UniRef:UniRef50_A0A448ZXC6", "COG:COG3887", "COG:T", "KEGG:K22927" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXC6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXC6" ], "product": "Probable manganese-dependent inorganic pyrophosphatase" } }, "id": "GLJLFKCOHC_889", "locus": "GCA013368035_00883" }, { "type": "cds", "sequence": "contig_1", "start": 505942, "stop": 506244, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAACAAAGATATTCAAAAAATTTATATAGCTTTACAAGTTATCCTATTATTTATTTTGCCAACAATATTATTCTTTATTGGCATTTTTTGTTTAAAGACTCAACAGTCAAAAATAGCGTTAATGGCAATTTACACTTTATTTTTAATCGGCCTTGCAATTTATTTAACTATTATTTTGCAACGTTATTTTAAAAACTCAATATTAGCTAAAAAATCATTGGAATTTTATTTACAAAGCGAATTTAATCAATTTGGTACGGGAATTTTGATATTTTTAAATAGTGGAAAAATTATATGA", "aa": "MKNKDIQKIYIALQVILLFILPTILFFIGIFCLKTQQSKIALMAIYTLFLIGLAIYLTIILQRYFKNSILAKKSLEFYLQSEFNQFGTGILIFLNSGKII", "aa_hexdigest": "93913c5e64e6e5d77a593477eabc9f08", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11558.992599999985, "isoelectric_point": 9.763881874084472 }, "id": "GLJLFKCOHC_890", "locus": "GCA013368035_00884" }, { "type": "cds", "sequence": "contig_1", "start": 506298, "stop": 506582, "strand": "-", "frame": 3, "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "db_xrefs": [ "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022", "SO:0001217", "UniRef:UniRef50_B3PN57", "UniRef:UniRef90_D1J8I2" ], "nt": "ATGATTGACTTTTATTTTAAAAATGATAAAAACAATAAAAAAGTGTTGCTATTAATTGATGCTAAAATTGGTTTTAGCGCTAGTGATTTAGAAATAATAGAATATCTTAACGATCTTAAAATAAAATTTGATATAGTTGTTACAAAAATCGACAAGGCAAATCAATCTCAACGTTATTGCGTTAAAAATCAAGCCTTGTCTTTAAAAACAGTTGAAAATATTTTTTTGGTATCATCTGAAAAAAAACAAGGTATTGAAGAAATAATGCAATTTTATGACTTGTAG", "aa": "MIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTVENIFLVSSEKKQGIEEIMQFYDL", "aa_hexdigest": "f71e398fe5467a9ae3882f5a394f00e0", "start_type": "ATG", "rbs_motif": null, "genes": [ "yihA" ], "truncated": "3-prime", "pseudogene": { "start": 506298, "stop": 506849, "observations": { "mutation": [ 506777, 506846 ], "stop": [ 506777, 506846 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I2", "query_cov": 1.0, "subject_cov": 0.5136612021857924, "identity": 1.0, "score": 184.0, "evalue": 7.77e-58, "gene_start": 1, "gene_end": 94, "reference_start": 90, "reference_end": 183, "reference_sequence": "MWKFLKSCKSHDDFINSDNIQYVFWGRSNVGKSSLINSLASCNIAKTSSYPGRTQLINYFESNKNKIIIDLPGYGYAKISKKLQDKISFMIDFYFKNDKNNKKVLLLIDAKIGFSASDLEIIEYLNDLKIKFDIVVTKIDKANQSQRYCVKNQALSLKTVENIFLVSSEKKQGIEEIMQFYDL" }, "paralog": false, "identity": 0.9890710382513661, "score": 354.0, "evalue": 2.02e-127, "description": "Internal stop codon at 506777, 506846. Nonsense mutation around 506777, 506846.", "psc": { "uniref90_id": "UniRef90_D1J8I2", "gene": "yihA", "product": "ribosome biogenesis GTP-binding protein YihA/YsxC", "uniref50_id": "UniRef50_B3PN57", "cog_id": "COG0218", "cog_category": "D", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I2", "UniRef:UniRef50_B3PN57", "COG:COG0218", "COG:D", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0042254", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN57", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN57" ], "product": "Probable GTP-binding protein EngB" } }, "id": "GLJLFKCOHC_891", "locus": "GCA013368035_00885" }, { "type": "cds", "sequence": "contig_1", "start": 506849, "stop": 507235, "strand": "-", "frame": 1, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAATTTAGAGATAAATACACAGAACATTTTACTATTCCACATACTGAAGCTCAATTGAACTTTTCATTTAGTGGATTAAAAACACAAGTTATAAATCTTATAAATAACCAAATCAATAGAAAACAAGAGATCAATGTTGATCAATATGCAACAATGTTTGAAAATACAGTAGTTTTATATTTAAAAAATCATTTAGAAATTGCAATTAAAAAATATCATCCAAGAGTAATTGTTTTGGCAGGCGGAGTAAGTGCTAATAAAGCAATTAGAGAAATGTTTTTAAATGTCCATTCAAATGCCATAATTCCGGAAATGAAATATACAACTGATAATGCAATGATGATTGCTAGGTTAGCATATGAACTATTAAGGAGAGATAGATAA", "aa": "MEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR", "aa_hexdigest": "e3577e7b0c508003aa3e90d94bf16fc9", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "3-prime", "pseudogene": { "start": 506849, "stop": 507535, "observations": { "mutation": [ 507238 ], "stop": [ 507238 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.4115755627009646, "identity": 1.0, "score": 252.0, "evalue": 3.28e-82, "gene_start": 1, "gene_end": 128, "reference_start": 184, "reference_end": 311, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9956140350877193, "score": 450.0, "evalue": 9.16e-163, "description": "Internal stop codon at 507238. Nonsense mutation around 507238.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "GLJLFKCOHC_892", "locus": "GCA013368035_00886" }, { "type": "cds", "sequence": "contig_1", "start": 507236, "stop": 507709, "strand": "-", "frame": 1, "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "db_xrefs": [ "COG:COG0533", "COG:J", "EC:2.3.1.234", "GO:0002949", "SO:0001217", "UniRef:UniRef50_Q6KIG0", "UniRef:UniRef90_D1J8I3" ], "nt": "ATGGAAACAATTAGCCAAATAGACATTCATAGAAAATATGGAGGCACAGTTCCGGAATTAGCATCAAGATTGCATGTAAAAAATATAGGTATTTTAATTAATAAATTAAAAAAAATAATTGATATTAAAAAGATTGACTATGTAGCATATACAAAAGAACCAGGATTAGTTGGTTCTTTACATGTTGGATATGTTGTTGCCAATGCAATAGCAATGTTACTTGAAAAACCTATATTGCCTTTAAATCATTTAGAAGGGCATTTTTTCTCTGCTTTTATAGGCAAAGAAGTTGAATATCCTGCTTTAGGTTTAATTGTTTCAGGTGGGCATTCACAATTAATGTTATATAAATCAAAAAATGAATACGAAGTTATAGGTCAAACTCAAGATGATGCCGTCGGAGAAGTATTTGATAAAATAGCAAGAAAATTAAATTTGGGTTTTCCTGGAGGACCTATTATTGATAAAAAATGA", "aa": "METISQIDIHRKYGGTVPELASRLHVKNIGILINKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKK", "aa_hexdigest": "310f3e03cec0d4050d30055b2f5b7d5d", "start_type": "ATG", "rbs_motif": null, "genes": [ "tsaD" ], "truncated": "both", "pseudogene": { "start": 506933, "stop": 507784, "observations": { "mutation": [ 507238, 507712 ], "stop": [ 507238, 507712 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I3", "query_cov": 1.0, "subject_cov": 0.5048231511254019, "identity": 0.9940000000000001, "score": 312.0, "evalue": 1.54e-105, "gene_start": 1, "gene_end": 157, "reference_start": 26, "reference_end": 182, "reference_sequence": "MLIFAIESSHDDTSFALLEDNKPIWMETISQIDIHRKYGGTVPELASRLHVKNIGILIEKLKKIIDIKKIDYVAYTKEPGLVGSLHVGYVVANAIAMLLEKPILPLNHLEGHFFSAFIGKEVEYPALGLIVSGGHSQLMLYKSKNEYEVIGQTQDDAVGEVFDKIARKLNLGFPGGPIIDKKWMEFRDKYTEHFTIPHTEAQLNFSFSGLKTQVINLINNQINRKQEINVDQYATMFENTVVLYLKNHLEIAIKKYHPRVIVLAGGVSANKAIREMFLNVHSNAIIPEMKYTTDNAMMIARLAYELLRRDR" }, "paralog": false, "identity": 0.9893992932862191, "score": 552.0, "evalue": 1.84e-202, "description": "Internal stop codon at 507238, 507712. Nonsense mutation around 507238, 507712.", "psc": { "uniref90_id": "UniRef90_D1J8I3", "gene": "tsaD", "product": "tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD", "ec_ids": [ "2.3.1.234" ], "uniref50_id": "UniRef50_Q6KIG0", "cog_id": "COG0533", "cog_category": "J", "go_ids": [ "GO:0002949" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I3", "EC:2.3.1.234", "UniRef:UniRef50_Q6KIG0", "COG:COG0533", "COG:J", "GO:0002949" ] }, "pscc": { "uniref50_id": "UniRef50_Q6KIG0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q6KIG0" ], "product": "tRNA N6-adenosine threonylcarbamoyltransferase" } }, "id": "GLJLFKCOHC_893", "locus": "GCA013368035_00887" }, { "type": "cds", "sequence": "contig_1", "start": 507778, "stop": 508332, "strand": "-", "frame": 2, "gene": "tsaB", "product": "Gcp-like domain-containing protein", "db_xrefs": [ "COG:COG1214", "COG:J", "RefSeq:WP_175949925.1", "SO:0001217", "UniParc:UPI00158D5627", "UniRef:UniRef100_UPI00158D5627", "UniRef:UniRef50_D1J8I4", "UniRef:UniRef90_D1J8I4" ], "nt": "GTGAAATTATTCATTGAAACTTCATTAAGCGATTTTTATATAAGCTTAATTGATGAAAATTATTGCGAGATTGATTGTATTTTTATTGCTAACTTAGTTAAAAAAACCGATGCATTTTTTGAAAATTTAAATTATTTATTAAATAAAAATAATTTTAAATTAAGTTCAATTTCAGAAATTTATACAACGTTAGGACCTGGGAGTTTTTCAGGTGCAAGAATTGGCTTAGTTTTTGCCAAAACAGTTGCACAATTATCAAATATCAAATTATTTGTAGCCCCAACATATTCTTTATTTTATACACAAAAAAAATTGCAATATGAAGATTGCAATTTAATAAATATAAAAGCCAATAAATACAATGTTTATTCAATAATTATTGATCCATTTGAATGTCATATGCAAGAAAACTTAGGCAATTTTGATTTATTCAATAAAGAGCTTTTTTCAAAAAATATAGAGCAATATTTAAAAGAATTTAAACAAGTAAATGATATTGAAAATTGCGAATTAATTTATTTGCATGATCCACAAATAGGAGAGATAAAATGTTAA", "aa": "MKLFIETSLSDFYISLIDENYCEIDCIFIANLVKKTDAFFENLNYLLNKNNFKLSSISEIYTTLGPGSFSGARIGLVFAKTVAQLSNIKLFVAPTYSLFYTQKKLQYEDCNLINIKANKYNVYSIIIDPFECHMQENLGNFDLFNKELFSKNIEQYLKEFKQVNDIENCELIYLHDPQIGEIKC", "aa_hexdigest": "cbe8a270f13ef18a39a1391107cf3b39", "start_type": "GTG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00158D5627", "ncbi_nrp_id": "WP_175949925.1", "uniref100_id": "UniRef100_UPI00158D5627", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D5627", "RefSeq:WP_175949925.1", "UniRef:UniRef100_UPI00158D5627" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D5627", "uniref90_id": "UniRef90_D1J8I4" }, "psc": { "uniref90_id": "UniRef90_D1J8I4", "gene": "TsaB", "product": "Gcp-like domain-containing protein", "uniref50_id": "UniRef50_D1J8I4", "cog_id": "COG1214", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_D1J8I4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8I4" ], "product": "Gcp-like domain-containing protein" }, "genes": [ "tsaB" ], "id": "GLJLFKCOHC_894", "locus": "GCA013368035_00888" }, { "type": "cds", "sequence": "contig_1", "start": 508434, "stop": 508730, "strand": "-", "frame": 3, "gene": "tsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "db_xrefs": [ "COG:COG0802", "COG:J", "SO:0001217", "UniRef:UniRef50_A0A3G8LG14", "UniRef:UniRef90_D1J8I5" ], "nt": "ATGTCAAATCTAAAATTTATTTATAAAAATAACGAACCATTAGATAAACTAGTTGACTATATTTTGTCATTTAAAAATTTAGAAGCCTTGCTTTTAATAGGCGAACTCGGAGCAGGCAAAACAACTTTAACAAGTCAAATAGCAAAAAAATTAAACGAACCAAAGACTGTAATATCACCCACATTCAATACGATTTTAGTGTATGATAAATTTGTACATATTGATGCTTATAAATTAACTACAAATTTATTTGCTTATGAAGATTATTTTGAAGATAAATTAGCAATTATTGAATGA", "aa": "MSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIE", "aa_hexdigest": "d4dfdacce5f9d287a456a83f5ae9929e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "tsaE" ], "truncated": "5-prime", "pseudogene": { "start": 508332, "stop": 508736, "observations": { "mutation": [ 508436 ], "stop": [ 508436 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8I5", "query_cov": 1.0, "subject_cov": 0.7313432835820896, "identity": 1.0, "score": 191.0, "evalue": 3.45e-61, "gene_start": 1, "gene_end": 98, "reference_start": 3, "reference_end": 100, "reference_sequence": "EIMSNLKFIYKNNEPLDKLVDYILSFKNLEALLLIGELGAGKTTLTSQIAKKLNEPKTVISPTFNTILVYDKFVHIDAYKLTTNLFAYEDYFEDKLAIIEWAENVTLPKFKHYIKIKVYFDNEQNHVFEVMEEQ" }, "paralog": false, "identity": 0.9925373134328358, "score": 265.0, "evalue": 1.15e-92, "description": "Internal stop codon at 508436. Nonsense mutation around 508436.", "psc": { "uniref90_id": "UniRef90_D1J8I5", "gene": "TsaE", "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE", "uniref50_id": "UniRef50_A0A3G8LG14", "cog_id": "COG0802", "cog_category": "J", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8I5", "UniRef:UniRef50_A0A3G8LG14", "COG:COG0802", "COG:J" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LG14", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LG14" ], "product": "tRNA threonylcarbamoyladenosine biosynthesis protein TsaE" } }, "id": "GLJLFKCOHC_895", "locus": "GCA013368035_00889" }, { "type": "cds", "sequence": "contig_1", "start": 508914, "stop": 509603, "strand": "-", "frame": 3, "gene": "ysdC", "product": "Aminopeptidase ysdC", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "nt": "ATGCAAGAAGAAGAAAGAAAGAAAGCAATTACAGAAAATGAATTATTTGCTGATTTTGGTTTCAAAAGCGATGAAGATGCTATTGCTCATGGTGTTAATGTTGGCGATAGAGTTTACATGAGTGGAGAAACAATACTATTTAATGATCAAAATTTAATTGGTGGAAAAGCAATGGACAACCGTGCTGGTGTAACCGTGTTAGAATTTGTTGCTAAAGCTGTTGCAAAATTAGATCTTGACATTGATCTATATTTAGTAGGTACAGTTCAAGAAGAAGTTGGAACAAGAGGAGCTAAAACAGTTGCGTCTATGATTGATCCAGACATTGCAATAGCAGTTGATACTTCTGCATCTCACGATACACCTAATACAATTCCTGGAAATGTTTCTTTATATAAGGGAGCTGCTTTAAGATTAAAAGATAGAGGAACAATGATGGATCCAAAATTGGTACAATTTTTTGTTGATTTATCTAAGAAATATAATATTCCAACATTTAAATATGTAGCCATGGGTGGGGGTACTGATGCCTTTGAATTGCAATTTTCAAAAGGCGGAGCAGCCACATTAACAATTTCATTGCCTCAAAGATATTTACATTCACCAATCGGAGTATGTTGCATAAGTGATTTATTGGCAGCCGTTGATTTGTTAACAAAATTTTTAACTGATTTTAAACAATCTGATTGA", "aa": "MQEEERKKAITENELFADFGFKSDEDAIAHGVNVGDRVYMSGETILFNDQNLIGGKAMDNRAGVTVLEFVAKAVAKLDLDIDLYLVGTVQEEVGTRGAKTVASMIDPDIAIAVDTSASHDTPNTIPGNVSLYKGAALRLKDRGTMMDPKLVQFFVDLSKKYNIPTFKYVAMGGGTDAFELQFSKGGAATLTISLPQRYLHSPIGVCCISDLLAAVDLLTKFLTDFKQSD", "aa_hexdigest": "2650e29473978c1d547374998b132b41", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002FE1AB9A", "query_cov": 0.8733624454148472, "subject_cov": 0.9478672985781991, "identity": 0.735, "score": 296.0, "evalue": 1.58e-99, "valid": false, "product": "M42 glutamyl aminopeptidase", "uniref50_id": "UniRef50_A0A3B0P1S5" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P1S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P1S5" ], "product": "Aminopeptidase ysdC" }, "genes": [ "ysdC" ], "id": "GLJLFKCOHC_896", "locus": "GCA013368035_00890" }, { "type": "cds", "sequence": "contig_1", "start": 509694, "stop": 509978, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAGAGAAAATCAATTCAAAGAAAGATTAATAAAATATATGGAAATTGAAGCAATGTCTCGTTATGAAGAACCAGTTGTTAATGAATTAAAAAAAGAATTAGCTAATGAAGGATTTGAATTTAGCAGAGATAATTTTGGCTGTTTAATTATGCACAAGCCATCTAAAAATCCAAATGCTCCTAAAGTCTTATTAGCAGCACACATGGATGAAGTTGGATATGTAGTAAGAAATTTTGATAAAAAAGGACAATTATATTTATCAACAGTTGGTGGTGTATGA", "aa": "MTRENQFKERLIKYMEIEAMSRYEEPVVNELKKELANEGFEFSRDNFGCLIMHKPSKNPNAPKVLLAAHMDEVGYVVRNFDKKGQLYLSTVGGV", "aa_hexdigest": "3fc428d1e9f98d963fffb68baebbdbb2", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10869.407299999992, "isoelectric_point": 6.733567619323731 }, "id": "GLJLFKCOHC_897", "locus": "GCA013368035_00891" }, { "type": "rRNA", "sequence": "contig_1", "start": 510108, "stop": 513003, "strand": "-", "gene": "rrl", "product": "23S ribosomal RNA", "coverage": 0.9900854700854701, "score": 2767.4, "evalue": 4.6e-06, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF02541", "KEGG:K01980", "SO:0001001" ], "nt": "TATTAAACAAGCAAGAGTTTTTGGTGGATGCCTTGGGTCTGGAAGTCGATGAAGGACGTGATTACCTGCGATAAGCCTCGGTTAGCTGGAAATAAGCTGTTATCCGGGGATTTCCGAATGGGGAAACCTAATTGAGCTAATCCTCAATTATCATATCGATGAATTCATAGTCGAATGAAGAGACACGCTGTGAATTGAAACATCTCAGTAGCAGCAGGAAGAGAAAATAAAGAATGATTCCCTAAGTAGTGGCGAGCGAACGGGGAAGAGCCCAAACCAACACATGTTGTTGGGGTTGTAGGACTGCGACATGGATTAATGAATTTGTACATAGCAGAATAAGTTGGAATGCTTAAACATAGAGGGTGAAATTCCCGTAAGCGAAATGTATAAATCTCCTAGCAGTATCCTGAGTAGGGCGGGGCACGTGAAACCCTGTCTGAATTTGCCGGGACCACCCGGTAAGGCTAAATACTAACCAGACACCGATAGTGAACTAGTACCGTGAGGGAAAGGTGAAAAGAACCCCGGGAGGGGAGTGAAATAGATTCTGAAACCAATTACTTACAGTTAGTCAGAGCCCGTTAATGGGTGATGGCGTACATCTTGCAGAATGGACCGGCGAGTTATGTCAACATGCGAGGTTAAGTAGAATAAAGCGAAGCCGTAGAGAAATCGAGTCTGAATAGGGCGATTTAGTATGTTGATATAGACCCGAAACCAGGTGATCTACCCATGAGCAGGTTGAAACTTAGGTAACACTAAGTGGAGGACCGAACCGCAGTACGCTAAAAAGTGCCCGGATGACTTGTGGGTAGGGGTGAAATTCCAATCGAACTTGGAGATAGCTGGTTCTCTCCGAAATAGCTTTAGGGCTAGCGTGTAGTGTTAAATGATGGGGGTAGAGCACTGAATATGGAATGGCGGCGCCTAGCCGTACTGACTATAATCAAACTCCGAATACTATCATGTATTACTATGCAGTCGGTACATCGGTGATAACGTCGATGCACGCGAGGGGAACAACCCAGATCGTCAGCTAAGGTCCCAAAATTGTGTTAAGTGAGAAAGGTTGTGAAGTTTCTTAAACAGCTAGGATGTTGGCTTAGAAGCAGCCACCGTTTAAAGAGTGCGTAATAGCTCACTAGTCGAGAGACTTTGTGCCGATAATTCAACGGGACTAAAACACAATACCGAAGCTACGGGCAGAAATGCGTTAGGAGAGCGTTGTAAGGGCTTAGAAGCCAGACCGTGAGGACTGGTGGAGCGCTTACAAGTGAGAATGCCGGTATGAGTAACGATTCAGAGTGAGAATCTCTGACGCCTATTGGGGAAGGTTTCCTGGGCAAGGTTCGTCCACCCAGGGTTAGTCGGGTCCTAAGACGAGGCCGAAAGGCGTAGCCGATGGACAACAGGTTAATATTCCTGTACTTTCTTAACATGTGATGGAGTGACGGGGAAGGATAATTTTACCACTAATTGGATTGTGGGGTAAGTAACAACTGGGTTATGTAGGCAAATCCGCATAACTTAACTGGGAGTTACGACGCATAGCAAAAGGGCAACTGAGTAGCGAATTAGATGATTTCATGCCTCTTAAAAAAGCTTCTAACATTAAGTGTTAGGAAACCCGTACCGAGAACGGACACACGTCCCCAAGATGAGTATTCTAAGGCGAGCGAGAAAACTATTGTCAAGGAACTCTGCAAATTCATCCCGTAAGTTCGCAAGAAGGGATGCCCACAATAAAATGTGGGCCGCAGTGAATAGTAAGGGGGAACTGTTTATCAAAAACACAGCTCTATGCTAAGTCGTAAGATGATGTATATGGGGTGACTCCTGCCCAGTGCCCGAAGGTTAAGCAGAGGTGTTAGCATTAGCGAAGCATTAATGTGAAGCCCGGGTGAACGGCGGCCGTAACTATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGCTAAATACTGACCTGCACGAAAGGAGTAATTATCTCTTAACTGTCTCGACAATAGACTCGGTGAAATTATGGTTCCGGCGAAGACGCCGGAGACCCGCATCTAGACGAAAAGACCCCGTGGAGCTTTACTATAACTTCATATTGGAGTTTGATTTAACATGTGTAGGATAGGTGGGAGACGTTGATGCTTGAACGCTAGTTTAAGAGTAGTCGCCGTTGAAATACCACCCTTGTTACATTGAACTTCTAACTTGTTCCCGTTATCCGGGAAGAGGACAGTGTGTGGTGGGTAGTTTGACTGGGGCGGTCGCCTCCTAAAGGGTAACGGAGGCGTTCAAAGTTACACTCAATACGGTCAGAAACCGTATCTAAGAGCATAAAGGTAGAAGTGTGATTGACTGTGAGACCTACAAGTCGAGCAGGTGCGAAAGCAGGACTTAGTGATCCGGCGGTTCTTCGTGGAAAGGCCGTCGCTCAACGGATAAAAGCTACCCCGGGGATAACAGGCTTATCTTTCCCAAGAGATCACATCGACGGGAAGGTTTGGCACCTCGATGTCGGCTCATCGCATCCTGGAGCTGGAGTCGGTTCCAAGGGTTGGGCTGTTCGCCCATTAAAGCGGTACGCGAGCTGGGTTCAAAACGTCGTGAGACAGTTTGGTTCCTATCTGATGTGGGCGTTGGAATATTGATGAGAGCTACTCTTAGTACGAGAGGACCGGAGTGGACGCACCGATGGTGTGCCAGTTGTTTCGCCAGAAGCATAGCTGGGTAGCCAAGTGCGGCAGGGATAACCGCTGAAAGCATCTAAGCGGGAAGCCCCCTCAAAGATTAGTATTCCCTTTAAAATTCCTTATAGACTATGAGGTTGATAGGCTGGAGGTGTAAGTGCAGCAATGCATTCAGCTGACCAGTACTAATAAATTTATTGGTTTAATAGT", "id": "GLJLFKCOHC_40", "locus": "GCA013368035_00892" }, { "type": "rRNA", "sequence": "contig_1", "start": 513186, "stop": 514703, "strand": "-", "gene": "rrs", "product": "16S ribosomal RNA", "coverage": 0.9902152641878669, "score": 1513.4, "evalue": 2.4e-46, "db_xrefs": [ "GO:0005840", "GO:0003735", "RFAM:RF00177", "KEGG:K01977", "SO:0001000" ], "nt": "TTTTATAAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCTGTGTGCCTAATACATGCATGTCGAGCGAGGTTAGCAATAACCTAGCGGCGAATGGGTGAGTAACACGTGCTTAATCTACCTTTTAGATTGGAATACCCATTGGAAACAATGGCTAATGCCGGATACGCATGGAACCGCATGGTTCCGTTGTGAAAGGCGCTGTAAGGCGCCACTAAAAGATGAGGGTGCGGAACATTAGTTAGTTGGTGAGGTAATGGCCCACCAAGACTATGATGTTTAGCCGGGTCGAGAGACTGAACGGCCACATTGGGACTGAGATACGGCCCAAACTCCTACGGGAGGCAGCAGTAGGGAATATTCCACAATGAGCGAAAGCTTGATGGAGCGACACAGCGTGCACGATGAAGGTCTTCGGATTGTAAAGTGCTGTTATAAGGGAAGAACATTTGCAATAGGAAATGATTGCAGACTGACGGTACCTTGTCAGAAAGCGATGGCTAACTATGTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGTTCGTAGGCTGTTTGTTAAGTCTGGAGTTAAATCCCGGGGCTCAACCCCGGCTCGCTTTGGATACTAGCAAACTAGAGTTAGATAGAGGTAAGCGGAATTCCATGTGAAGCGGTGAAATGCGTAGATATATGGAAGAACACCAAAGGCGAAGGCAGCTTACTGGGTCTATACTGACGCTGAGGGACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGCCGTAAACGATGATCATTAGTCGGTGGAGAATCACTGACGCAGCTAACGCATTAAATGATCCGCCTGAGTAGTATGCTCGCAAGAGTGAAACTTAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTTGAAGATACACGGAAAACCTTACCCACTCTTGACATCCTTCGCAAAGCTATAGAGATATAGTGGAGGTTATCGGAGTGACAGATGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTTGGTCAAGTCCTGCAACGAGCGCAACCCCTATCTTTAGTTACTAACATTAAGTTGAGGACTCTAGAGATACTGCCTGGGTAACTGGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCTCTTACGAGTGGGGCCACACACGTGCTACAATGGTCGGTACAAAGAGAAGCAATATGGCGACATGGAGCAAATCTCAAAAAGCCGATCTCAGTTCGGATTGGAGTCTGCAATTCGACTCCATGAAGTCGGAATCGCTAGTAATCGCAGATCAGCTATGCTGCGGTGAATACGTTCTCGGGTCTTGTACACACCGCCCGTCACACCATGGGAGCTGGTAATACCCAAAGTCGGTTTGCTAACCTCGGAGGCGACCGCCTAAGGTAGGACTGGTGACTGGGGTGAAGTCGTAACAAGGTATCCCTACGAGAACGTGGGGATGGATCACCTCCTTT", "id": "GLJLFKCOHC_36", "locus": "GCA013368035_00893" }, { "type": "cds", "sequence": "contig_1", "start": 514980, "stop": 515207, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGATAAAGAAGCAATGTTGCTAGCTAAATTTAAAGCAGGGGACATTGAAACTGCTTTTAAAGAAGGGGCTTTAGTAAAAATTTCTAGCGGAATATTCAAAGATGAAGTTGGCGAAATAATTAAAAATAATGATATTACCCAAAAAGCCTTTGTTAATATTGAACAATTCGGTAGAAAAGTTCCAACTGAATTTGATTATGCAGATCTAGAAATAGTAGGTTAA", "aa": "MIDKEAMLLAKFKAGDIETAFKEGALVKISSGIFKDEVGEIIKNNDITQKAFVNIEQFGRKVPTEFDYADLEIVG", "aa_hexdigest": "6cd0e41740e267b0c0a651441ba51b2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8339.500699999999, "isoelectric_point": 4.7058935165405265 }, "id": "GLJLFKCOHC_898", "locus": "GCA013368035_00894" }, { "type": "cds", "sequence": "contig_1", "start": 515304, "stop": 515534, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02357.24" ], "nt": "ATGATTTCAACAGTTTCAGGAAAAGAAGAAAATGTTATAGAATCATTAAAAAATAAAATTGCTGCTCAAAGAATGGACGAATACTTTAAAGATATAAGAATGTTTAAAGAACCCCATTTATCAAATAAAGAATTAGAAAAGAAGCAAAGAAACGAAGAATTTACTGTTAAATTTGTAAATATGTACAAAGGTTATATTTTCTTAAATATGGCAATGACTGATGATACATGA", "aa": "MISTVSGKEENVIESLKNKIAAQRMDEYFKDIRMFKEPHLSNKELEKKQRNEEFTVKFVNMYKGYIFLNMAMTDDT", "aa_hexdigest": "83445adab5e4fdbba14afdcaba235a99", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02357.24", "name": "NusG", "length": 74, "aa_cov": 0.9736842105263158, "hmm_cov": 0.7432432432432432, "evalue": 2.2408898826897654e-08, "score": 29.945831298828125, "start": 2, "stop": 75 } ], "seq_stats": { "molecular_weight": 9073.367599999994, "isoelectric_point": 6.579306602478028 }, "id": "GLJLFKCOHC_899", "locus": "GCA013368035_00895" }, { "type": "cds", "sequence": "contig_1", "start": 516586, "stop": 516699, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAGGTGAACATGAACATTTTCCTCAACACTTTATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAATGATGAATATCTAAAATGA", "aa": "MAGEHEHFPQHFIDNLKDKKTIIQKKLVDFNNDEYLK", "aa_hexdigest": "2c6895ab2ac8430c7ff67353142c79f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4485.040300000001, "isoelectric_point": 6.235260200500488 }, "id": "GLJLFKCOHC_900", "locus": "GCA013368035_00896" }, { "type": "cds", "sequence": "contig_1", "start": 516883, "stop": 517236, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGGAAAAGAAATTTAAATTTAATTATGAAATAGAAGTTGAAAAAATAAAAAGTTTGATTAACAAATGTCAAAATTCAAAAGACGTTTCCAATATTGAACAAATTATTAATAAACTATTGGCTTCAATAAATACAACTAGTATAAAAATCTATAACTATATTAAAACCTTTGCCTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGATATTCAAAATAAAAAAATTTCTGATTTTTTTGCAAGTGAAACTAAAAAGAATTTATTAAAAAGTGAATACCTTAATACTTATAAGCCAATATTGAATAGTTTTATAGATATTAAAAATAATAATTGA", "aa": "MLEKKFKFNYEIEVEKIKSLINKCQNSKDVSNIEQIINKLLASINTTSIKIYNYIKTFAFHLKIQNNIKKIIEKIDIQNKKISDFFASETKKNLLKSEYLNTYKPILNSFIDIKNNN", "aa_hexdigest": "78ea542096114272eef4b2f97e127a13", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13838.010899999976, "isoelectric_point": 9.583950614929197 }, "id": "GLJLFKCOHC_901", "locus": "GCA013368035_00897" }, { "type": "cds", "sequence": "contig_1", "start": 517291, "stop": 517725, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410F5F0", "UniRef:UniRef90_UPI002410F5F0" ], "nt": "TTGCTAAGGGTTCAAAATAAAATAATAAATAAAATAGATGAACAAGAATATCGAAACAATCAAATTGAATTCTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAGTATTAAATATTAGTTTAAATCCAATAGACATTGAAAACATAAATAGTTTATTTACTTTTATTTATAAATTCAAAAGTAATGGTTTTTTTGCAAATGAATTGAATAATTTTTTAAGTTTTTTATCTAATGAAATAAATTTTTTAAGTTGCAAAATTCAATATTTTTTAATAATTAAAAATGTAGAATTTTCTTTAAGAAAAGACGTAAGTGAAAATTCAAATGCCCAAAATTTATTTAATGAGTTGCAAAAACTTAAAGGTTACAATGATGATAAAAAAGCAATTGATTTAATATATGATTATATAGAAGAACAATACAACTAA", "aa": "MLRVQNKIINKIDEQEYRNNQIEFYIENFKRLTANIKVLNISLNPIDIENINSLFTFIYKFKSNGFFANELNNFLSFLSNEINFLSCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN", "aa_hexdigest": "2554c885fdcd8e50a3071f7adda261dd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 516991, "stop": 517725, "observations": { "mutation": [ 517234, 517270 ], "stop": [ 517234, 517270 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002410F5F0", "query_cov": 1.0, "subject_cov": 0.5517241379310345, "identity": 0.903, "score": 252.0, "evalue": 1.28e-82, "gene_start": 1, "gene_end": 144, "reference_start": 118, "reference_end": 261, "reference_sequence": "MINKCQNSKDISNIEQIINKLLALINTTSIKIYNYIKTFNFHLKIQNNIKKIIEKIDVQNKKISDFFASETKKNLLKSEYLNTYKPILNSFIDIKNNNWMDIVEFIELKNWLESLLNLLRIQNKIINKIDEQEYRNNQIEFYIENFKRLTANIKILNISLSSEDIENINNLFAFIYKFKNNNFSANELNDFLSYLSNEINFLNCKIQYFLIIKNVEFSLRKDVSENSNAQNLFNELQKLKGYNDDKKAIDLIYDYIEEQYN" }, "paralog": false, "identity": 0.9139344262295082, "score": 440.0, "evalue": 3.6e-159, "description": "Internal stop codon at 517234, 517270. Nonsense mutation around 517234, 517270.", "psc": { "uniref90_id": "UniRef90_UPI002410F5F0", "uniref50_id": "UniRef50_UPI002410F5F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002410F5F0", "UniRef:UniRef50_UPI002410F5F0" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002410F5F0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002410F5F0" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 17250.383599999986, "isoelectric_point": 5.263995552062987 }, "id": "GLJLFKCOHC_902", "locus": "GCA013368035_00898" }, { "type": "cds", "sequence": "contig_1", "start": 517736, "stop": 517834, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF22025.1" ], "nt": "ATGGACTACAATAAAATTTTAATTAGATATGGTGAATTGACTCTTAAGGGAGAAAATAAAAAAGATTTTATTAGACAATTGCATAACAACCTATCTTGA", "aa": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLS", "aa_hexdigest": "fadac77c9c73137a76016e2ce1732acf", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF22025.1", "name": "ThiI_fer", "length": 26, "aa_cov": 0.8125, "hmm_cov": 1.0, "evalue": 4.6413672710607855e-12, "score": 41.67063522338867, "start": 6, "stop": 31 } ], "seq_stats": { "molecular_weight": 3865.4184000000005, "isoelectric_point": 9.301772499084471 }, "id": "GLJLFKCOHC_903", "locus": "GCA013368035_00899" }, { "type": "cds", "sequence": "contig_1", "start": 518063, "stop": 518884, "strand": "+", "frame": 2, "gene": "thiI", "product": "putative tRNA sulfurtransferase", "db_xrefs": [ "COG:COG0301", "COG:HJ", "SO:0001217", "UniRef:UniRef50_B3PLZ2", "UniRef:UniRef90_D1J8J1" ], "nt": "ATGACTTCAAGTGAACTAAATATGTATTTTGGAGGTATTGTTTTAAAACAAATGCCAGATAAAGAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGATGGCTTAGGTGGTATGCCGGTATTGAGTTCTGGAAAATGCATACATCTAATTAGTGGGGGCATTGACTCGCCTGTAGCAGCAAACTTATTACAAAAAAGAGGTTTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACAGATGAAAAAACAATTGCAAAAATAGATGAAATTGTTAATATACTAAAAAAATATCAAGGCAATGCTATGTTATATCAAATTAACTATAGCAAAGTAATGGAATATATTGGCCTTATAAGTAATCAAAAATACAAAATAACTTTAATGAGAAGATCTTTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGTGAAAATTTAGCCCAAGTTGCATCTCAAACTCTTGAATCTATTTATACAATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCTTTAATTTCTTTTGACAAAAATGAAACTATTGCTATTGCAAAAAAAATTAGCACATTAGATGTTTCAATAGTAAGGGCGTGTGAAACATGTGAATTATTTGCTCCTAAAAATCCTGTAACCAAGCCTAATAGGCAAGAAGCTCATAAACTCGAAGAAGAAGAATTGCCTTTGATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAA", "aa": "MTSSELNMYFGGIVLKQMPDKEVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKISTLDVSIVRACETCELFAPKNPVTKPNRQEAHKLEEEELPLIKEFEEECIKNAIIKKYSI", "aa_hexdigest": "69851ba54466c31e4ab90a90ef7a368c", "start_type": "ATG", "rbs_motif": null, "genes": [ "thiI" ], "truncated": "5-prime", "pseudogene": { "start": 517763, "stop": 518884, "observations": { "mutation": [ 517832 ], "stop": [ 517832 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J1", "query_cov": 1.0, "subject_cov": 0.7146596858638743, "identity": 0.982, "score": 526.0, "evalue": 1.02e-186, "gene_start": 1, "gene_end": 273, "reference_start": 110, "reference_end": 382, "reference_sequence": "MDYNKILIRYGELTLKGENKKDFIRQLHNNLSWYIEKSEIKTEYDRAFVDYSQKNLDCLKYIFGISSYSPVYETETSLINIENIVKEIISKKQFDSFAINARRHNKNFSMTSSELNMYFGGIVLKQMPDKQVKLKNPDLTIYIEIRDQRSFVFCDYFDGLGGMPVLSSGKCIHLISGGIDSPVAANLLQKRGLKIVFLNFITPPQTDEKTIAKIDEIVNILKKYQGNAMLYQINYSKVMEYIGLISNQKYKITLMRRSFYRIANLIAKKENIKAISNGENLAQVASQTLESIYTISEVCDLPIFRPLISFDKNETIAIAKKINTLDVSIVRACETCELFAPKNPVTKPNRQEAYKLEEEELPLIKEFENECIENAIIKKYSI" }, "paralog": false, "identity": 0.9731903485254692, "score": 719.0, "evalue": 1.37e-265, "description": "Internal stop codon at 517832. Nonsense mutation around 517832.", "psc": { "uniref90_id": "UniRef90_D1J8J1", "gene": "ThiI", "product": "Probable tRNA sulfurtransferase", "uniref50_id": "UniRef50_B3PLZ2", "cog_id": "COG0301", "cog_category": "HJ", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J1", "UniRef:UniRef50_B3PLZ2", "COG:COG0301", "COG:HJ" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLZ2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLZ2" ], "product": "Probable tRNA sulfurtransferase" } }, "id": "GLJLFKCOHC_904", "locus": "GCA013368035_00900" }, { "type": "cds", "sequence": "contig_1", "start": 518937, "stop": 519050, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "nt": "ATGCCTCCAACAGGCGGTTCTGGTATAGGAATTGATCGTTTAATAATGCTATTAACTCAAAATGATTCAATTAGAGATGTAGTTTTATTCCCTCAATTAAAAGATTTAAAATAA", "aa": "MPPTGGSGIGIDRLIMLLTQNDSIRDVVLFPQLKDLK", "aa_hexdigest": "d9b52268f805181a0d249c6481f62c51", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A920J7V4", "query_cov": 0.8378378378378378, "subject_cov": 0.8611111111111112, "identity": 0.677, "score": 46.2, "evalue": 5.74e-06, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A920J7V4" }, "pscc": { "uniref50_id": "UniRef50_A0A920J7V4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A920J7V4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_905", "locus": "GCA013368035_00901" }, { "type": "cds", "sequence": "contig_1", "start": 519078, "stop": 519575, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGGAAAGAACTGAAGGTGTTTTCAAACATTTAATAAATAAATTCAATATTAAAGAAGTTAAATTTAATGGCTTTGATATAACATTTAATTTTCCATTTAAGAGAGTCAATATGATTGATGCTATATCGGAAAAAATTAATGTTGACATTAGAAAATTAAATGAAGAACAAATGTTTGAATTAGCTAAAAAACATGGTATTAAAGTTGAAAAATTCTTTAGATATGGTCACATCATCAATGAGTTATTTGAAAAATATGTTGAAGAAACATTAATAGAACCAACATTTGTATATGGCCACCCAATTGAAATTTCTCCTCTTGCAGCAATTGACCCTGACGATAAAAGATTTACACTACGTGCTGAACTATTTATTGGAACAAAAGAATTTGCAAATATGTTTACTGAATTAAATGATCCAATTGACCAATTGCAACGTTTTGAAAACCAATTGTTGGAAAAAGAAAACGGTAACGAAGAAGCAAATGAAATTGATTGA", "aa": "MERTEGVFKHLINKFNIKEVKFNGFDITFNFPFKRVNMIDAISEKINVDIRKLNEEQMFELAKKHGIKVEKFFRYGHIINELFEKYVEETLIEPTFVYGHPIEISPLAAIDPDDKRFTLRAELFIGTKEFANMFTELNDPIDQLQRFENQLLEKENGNEEANEID", "aa_hexdigest": "63d8fd55075fd618c782f9a6c8b2305e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0027D2801B", "query_cov": 0.9030303030303031, "subject_cov": 0.8097826086956522, "identity": 0.53, "score": 147.0, "evalue": 4.83e-42, "valid": false, "product": "amino acid--tRNA ligase-related protein", "uniref50_id": "UniRef50_A0AA38HFP6" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 165, "aa_cov": 0.9636363636363636, "hmm_cov": 0.806060606060606, "evalue": 7.319330198221772e-15, "score": 50.169349670410156, "start": 1, "stop": 159 } ], "seq_stats": { "molecular_weight": 19551.041799999995, "isoelectric_point": 4.986791801452636 }, "id": "GLJLFKCOHC_906", "locus": "GCA013368035_00902" }, { "type": "cds", "sequence": "contig_1", "start": 519582, "stop": 520403, "strand": "-", "frame": 3, "gene": null, "product": "amino acid--tRNA ligase-related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "nt": "ATGGAAAGAAAACTTAGTGAACAAGAAGAAATTAGACGTAATAAAATTGCTGAATTAGAAAAAAACAATTTACAAGCATTTCACAAAACTATAAAACCAACAACAAATTCAAAAGAGTTAAATGAAAAATATAAAAATTTAAGCCGTGAAGATCTTGACAAACAAAATTTAGAATTTGTATTTAATGGAAGGATTATGACTATTCGTGGCCCATTTATTGTTTTACAAGATAATTGCGGACAAATGCAAATTTATGCAAACAAAAAAACATTGAATGAGTCTTCTACAAAAACCTTTTCTTTAATGGATTTTGGCGATATTTTAAGTGTAAGAGGAACATTATCAAAAACTAATACTGATGCTTTGGTTATCAAAGCTCAAGACATAGTTCTATTAACAAAATCATTGAAACCCTTACCTGATAAATTTCATGGTTTAGTTGATCCAGAAGAAAGACGTCGTCAACGCTATGTAGATTTAATAATGAATGAAAAAACAAGAAATACATTTGTTATGAGAACCCAAATTATCAAAGGCATTAGAGAATATTTTGATAGTTTGAACTACATTGAAGTTGATACACCAATTTTAAATCCAATATTAGGAGGAGCAGCTGCTAAACCTTTTATTACTTTCTATAATGCACTTAATAGAAACTTTTATTTAAGAATTGCAACTGAATTGCCATTAAAAAAATTGATTGTTGGTGGATTAGATAGAGTTTATGAAATCGGTAGAATATTTAGAAATGAAGGCGTAGATACAACTCATAATCCAGAATTTACCTCAATTGAATTTTATGAAGCATATTCAGATTTATGA", "aa": "MERKLSEQEEIRRNKIAELEKNNLQAFHKTIKPTTNSKELNEKYKNLSREDLDKQNLEFVFNGRIMTIRGPFIVLQDNCGQMQIYANKKTLNESSTKTFSLMDFGDILSVRGTLSKTNTDALVIKAQDIVLLTKSLKPLPDKFHGLVDPEERRRQRYVDLIMNEKTRNTFVMRTQIIKGIREYFDSLNYIEVDTPILNPILGGAAAKPFITFYNALNRNFYLRIATELPLKKLIVGGLDRVYEIGRIFRNEGVDTTHNPEFTSIEFYEAYSDL", "aa_hexdigest": "02313c799feaf7cc566e63620b3ed94a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001EED3E5F", "query_cov": 0.9926739926739927, "subject_cov": 0.8885245901639345, "identity": 0.546, "score": 291.0, "evalue": 1.86e-95, "valid": false, "product": "amino acid--tRNA ligase-related protein", "ec_ids": [ "6.1.1.6" ], "uniref50_id": "UniRef50_UPI001EED3E5F", "kegg_orthology_id": "K04567" }, "pscc": { "uniref50_id": "UniRef50_UPI001EED3E5F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001EED3E5F" ], "product": "amino acid--tRNA ligase-related protein" }, "genes": [], "id": "GLJLFKCOHC_907", "locus": "GCA013368035_00903" }, { "type": "tRNA", "sequence": "contig_1", "start": 520495, "stop": 520569, "strand": "+", "gene": "trnR", "product": "tRNA-Arg(cct)", "amino_acid": "Arg", "anti_codon": "cct", "score": 68.0, "nt": "GGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 520524, 520526 ], "id": "GLJLFKCOHC_23", "locus": "GCA013368035_00904" }, { "type": "cds", "sequence": "contig_1", "start": 520840, "stop": 521022, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTGGACATCCAATCATTCTTTGGCAATGAACAAGGAATGAATTTTGTTGAACTTGATGAAAGCAAAAGAAAAGCTCACAAATTTTATGCCAAAATTTTCTTTATAAGTATCGCAGTTTTAATTGTATTGCCCTTTATAATATATATTATTTTAAAGAAGACAGTATTGAAAGGAAAGTAG", "aa": "MVDIQSFFGNEQGMNFVELDESKRKAHKFYAKIFFISIAVLIVLPFIIYIILKKTVLKGK", "aa_hexdigest": "3e48426f9f9a4a35211fe1f188288b60", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6981.354099999997, "isoelectric_point": 9.631657218933103 }, "id": "GLJLFKCOHC_908", "locus": "GCA013368035_00905" }, { "type": "cds", "sequence": "contig_1", "start": 521309, "stop": 521479, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAATTCTATACATATCTATGCTTTTATGTATTCCTGAAAGTTCAATCAATTCAATAGTAAATCTAGTTAACGAAATTACTATAAATATTGCTGGAATAATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTGAATAAATTTGGTCAAAAGTAA", "aa": "MVILYISMLLCIPESSINSIVNLVNEITINIAGIIYQAHFILKSFALEKLNKFGQK", "aa_hexdigest": "ff158cb0bfeffc849acb0c5cfd576242", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6310.531599999998, "isoelectric_point": 7.907514762878418 }, "id": "GLJLFKCOHC_909", "locus": "GCA013368035_00906" }, { "type": "cds", "sequence": "contig_1", "start": 521524, "stop": 521745, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350", "UniRef:UniRef90_UPI001CC77350" ], "nt": "ATGGATATTAAGCCCACAACAACAGGAAATAATAGCGATAGTAGTAAAAACATTGAAATTCCAACACGTACCTTTGAAAATCACATGGTAGAAGATTCAATGAATACTCAAAATTTGCAACAAATAAGTTACAAAATAAAAGATCCTCAGAAAATAATTCCTAATGGAATTTATTCAGTCTTTCAAAGAGAAATGAATATTAAAAAAATATCAATACTTTGA", "aa": "MDIKPTTTGNNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQISYKIKDPQKIIPNGIYSVFQREMNIKKISIL", "aa_hexdigest": "a5a690f6d6d8a6b1d3f044d532eebcbb", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 521391, "stop": 521745, "observations": { "insertion": [ 521421 ], "mutation": [ 521472, 521526 ], "stop": [ 521472, 521526 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001CC77350", "query_cov": 1.0, "subject_cov": 0.6239316239316239, "identity": 0.973, "score": 140.0, "evalue": 9.41e-42, "gene_start": 1, "gene_end": 73, "reference_start": 1, "reference_end": 73, "reference_sequence": "MDIKPTTTGSNSDSSKNIEIPTRTFENHMVEDSMNTQNLQQLSYKIKDPQKIIPNGIYSVFQREMNIKKISILWNFGILLLSVIAILLLYFWPNLFNFSNAKDFKIKWLDILFRQYL" }, "paralog": false, "identity": 0.9487179487179487, "score": 216.0, "evalue": 5.17e-74, "description": "Internal stop codon at 521472, 521526. Frameshift due to insertion around 521421. Nonsense mutation around 521472, 521526.", "psc": { "uniref90_id": "UniRef90_UPI001CC77350", "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001CC77350", "UniRef:UniRef50_UPI001CC77350" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001CC77350", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001CC77350" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8428.521199999997, "isoelectric_point": 8.030133628845213 }, "id": "GLJLFKCOHC_910", "locus": "GCA013368035_00907" }, { "type": "cds", "sequence": "contig_1", "start": 521752, "stop": 522516, "strand": "-", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGGATATTCTTCAAGAAAGACTCGAAAGAGAATTTAATTTAGATATTATTGCTACTGCTCCTTCAGTTGAGTTTGTTGTAACACTAACCAATGGGGACGTTCAATATATTACAAATCCTTCACTATTTCCAGACAGAAGTTATATAAAAATGATAGAGGAACCTTATATTAAGGCTAATATAATGTTAACCGAAGAATATTTGGGCCCAATTATGGAATTATGCCAATCTAAGCGTGGTAAATATGTAAATATTGAATATTTAGATGAAACTCGCAGATTATTGATTTATGAATTGCCTTTAAATGAAATAATATTTGATTTCTTTGATTTAATGAAGAGCTATTCAAAAGGTTATGCTTCATTTGATTATGACTACATTGGTTTAAGAGAAAGCGATTTAGTAAAGGTTGATATTTTATTAAATGGTGAGAAAATTGATGCCTTAGCAATGATTGTCCATAAAGACTCGGCATATAATAAAAGTAGAGCATTAACAGAAAAATTAAAGGAAGTTGTACCAAGACAAAATTTTGAAATTCCCGTTCAAGCTGCAATTGGTGCTAAAATTATAGCAAGAGAAACAATTAAGGCATATAGAAAAGATGTTACAGCTAAGTTATATGGTGGTGACGTTACAAGAAGACAAAAACTATTAAAAAAACAAAAAATAGGTAAAAAAAGGATGAAACAAATAGGAAGCGTTGAAGTTCCACAAGAAGCATTTTTGGCAATATTAAAAACAGATGATTCTCGAAAGGATTAG", "aa": "MDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHKDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD", "aa_hexdigest": "8f107582d8e501a141d9db8374051d14", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A449BBE2", "query_cov": 1.0, "subject_cov": 0.8639455782312925, "identity": 0.685, "score": 343.0, "evalue": 2.85e-116, "valid": false, "product": "GTP-binding protein lepA", "uniref50_id": "UniRef50_A0A449BBE2" }, "genes": [ "lepA" ], "truncated": "3-prime", "pseudogene": { "start": 521752, "stop": 522816, "observations": { "mutation": [ 522582, 522789 ], "stop": [ 522582, 522789 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.996, "score": 488.0, "evalue": 8.87e-169, "gene_start": 1, "gene_end": 254, "reference_start": 346, "reference_end": 599, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9915254237288136, "score": 686.0, "evalue": 2.23e-249, "description": "Internal stop codon at 522582, 522789. Nonsense mutation around 522582, 522789.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "GLJLFKCOHC_911", "locus": "GCA013368035_00908" }, { "type": "cds", "sequence": "contig_1", "start": 522580, "stop": 522705, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAGGATATAAAAAAATAAAACCAGTTGTTTATACTGGATTTTATCCTGTCGATACTAGAGATTACAATGATTTAAAAGATGCCCTAGAAAAAATTAGTTTATCTGATTCTTCATTGGTATGA", "aa": "MPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLV", "aa_hexdigest": "4a49afffe937ca97bc817e164d8b878b", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4667.291399999999, "isoelectric_point": 5.986134910583496 }, "id": "GLJLFKCOHC_912", "locus": "GCA013368035_00909" }, { "type": "cds", "sequence": "contig_1", "start": 522787, "stop": 523551, "strand": "-", "frame": 2, "gene": "lepA", "product": "translation elongation factor 4", "db_xrefs": [ "EC:3.6.5.n1", "GO:0003924", "GO:0005525", "SO:0001217", "UniRef:UniRef50_Q9ZHZ8", "UniRef:UniRef90_A0A454CA39" ], "nt": "ATGACAAACGAAAAAATAAGAAATTTTGCAATAATAGCACACATTGATCACGGTAAAAGTACTTTAGCTGATCGTATTTTGGAATTTACCAATACAATTTCTGCCAGGGAAGCAAAACCTCAAATTTTAGATTCAATGGAACTAGAACAAGAACGTGGAATCACCATCAAATTAAATGCTATTCAAATTAAATATAAAGATTACATTTTTCACCTGATAGATACACCAGGCCATGTTGATTTTACTTATGAGGTTAGTAGGTCACTTGCTGCATGTGAAGGAGCATTATTGCTAGTTGATGCAACTCAAGGAATTCAAGCACAAACATTGGCTAATGTTTATATGGCAATGGAAAATAATTTAGAAATCGTTCCTATTATTAATAAGGTAGATTTAATTAGTGCAGATCCCGAAAGAGTAAAAAAAGAAATTCAAGACATGATTGGAATTGATGCTTCTGATGCAATTATGATTTCAGCAAAGACAGGATTGAATATTGATAAAGTAATTGATGCAATTATAAACAAAATCCCCGCTCCTAAAGAAGCAGATATAAATAAACCATTGCAAGCTTTAATTTTTGATAGCTATTTTGACAATTATCGTGGCGTTGTTATCTATGTGAGATTGTTTGATGGCAAAGTAAAAGTAGGCGACGAAATTTACTTTATGCAAACAGAAAAGCATTATTTAGTAACGGAACTAGGCGTTAAAAATCCTAATGAAGTTCAAAAAAATGAATTGCAAGCTGGCGAAGTTGGTTGA", "aa": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDASDAIMISAKTGLNIDKVIDAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVG", "aa_hexdigest": "c30ab95eaf3fd539c9a8970ed0e6cc76", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00317F3865", "query_cov": 1.0078740157480315, "subject_cov": 0.8827586206896552, "identity": 0.633, "score": 325.0, "evalue": 4.9e-109, "valid": false, "product": "GTP-binding protein", "uniref50_id": "UniRef50_A0A0P1NWJ7" }, "genes": [ "lepA" ], "truncated": "5-prime", "pseudogene": { "start": 522484, "stop": 523551, "observations": { "mutation": [ 522582, 522789 ], "stop": [ 522582, 522789 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA39", "query_cov": 1.0, "subject_cov": 0.4240400667779633, "identity": 0.992, "score": 489.0, "evalue": 2.2e-169, "gene_start": 1, "gene_end": 254, "reference_start": 1, "reference_end": 254, "reference_sequence": "MTNEKIRNFAIIAHIDHGKSTLADRILEFTNTISAREAKPQILDSMELEQERGITIKLNAIQIKYKDYIFHLIDTPGHVDFTYEVSRSLAACEGALLLVDATQGIQAQTLANVYMAMENNLEIVPIINKVDLISADPERVKKEIQDMIGIDTSDAIMISAKTGLNIDKVIHAIINKIPAPKEADINKPLQALIFDSYFDNYRGVVIYVRLFDGKVKVGDEIYFMQTEKHYLVTELGVKNPNEVQKNELQAGEVGWISASIRDIKDVEVGDTVTLFENQCPEPLPGYKKIKPVVYTGFYPVDTRDYNDLKDALEKISLSDSSLVWQPETSKALGFGFRIGFLGLLHMDILQERLEREFNLDIIATAPSVEFVVTLTNGDVQYITNPSLFPDRSYIKMIEEPYIKANIMLTEEYLGPIMELCQSKRGKYVNIEYLDETRRLLIYELPLNEIIFDFFDLMKSYSKGYASFDYDYIGLRESDLVKVDILLNGEKIDALAMIVHRDSAYNKSRALTEKLKEVVPRQNFEIPVQAAIGAKIIARETIKAYRKDVTAKLYGGDVTRRQKLLKKQKIGKKRMKQIGSVEVPQEAFLAILKTDDSRKD" }, "paralog": false, "identity": 0.9887323943661972, "score": 685.0, "evalue": 6.36e-249, "description": "Internal stop codon at 522582, 522789. Nonsense mutation around 522582, 522789.", "psc": { "uniref90_id": "UniRef90_A0A454CA39", "gene": "lepA", "product": "translation elongation factor 4", "ec_ids": [ "3.6.5.n1" ], "uniref50_id": "UniRef50_Q9ZHZ8", "go_ids": [ "GO:0003924", "GO:0005525" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA39", "EC:3.6.5.n1", "UniRef:UniRef50_Q9ZHZ8", "GO:0003924", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_Q9ZHZ8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9ZHZ8" ], "product": "Elongation factor 4" } }, "id": "GLJLFKCOHC_913", "locus": "GCA013368035_00910" }, { "type": "cds", "sequence": "contig_1", "start": 523651, "stop": 524847, "strand": "-", "frame": 2, "gene": null, "product": "endopeptidase La", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9X8" ], "nt": "TTGGGCGGTGTTAAGGATGAAAGTGAAATAAGAGGACACAGAAGAACTTATGTTGGGGCAATGCCTGGAAAAATAATATCAGCAATTAAAAAAGCCGGAGTTTCAAACCCAGTCATTTTATTAGATGAAATTGATAAGATGTCATCAGATTTTCGAGGCGATCCAACAAGCGCAATGCTTGAAGTATTAGACCCTGAACAAAATGTTAATTTTCAAGATCACTACTTGGATTTAGAATATGACTTATCAAAAGTATTATTCATCGCTACTGCTAACTACTATGATTCAATACCCGCAGCACTAATTGATCGTGTTGAATTACTTGAACTATCAACATATACAACTAAAGAAAAAGTAGAAATTGCTAAATCGCATTTACTTCCAAAAGTGTATGAACAAAATGCCTTAAATCCTGAACAATTTCAAATTTCTGACGAAGTTTTAGAATTTTTAGTTCGTTACTACACAAGGGAAAGTGGAGTTCGTGAATTAAAACGTATTTTAGATAGTATAGCAAGAAAGATTGTTGTCGAAATTCTTGACAAAAAAATCAACGATAGTTTTGTTGTTACAAAAGAAGTTGTAATTGAATTTTTAGGACCAATTAAATTCGATGATGATGAAAATGAAAATAAGGCACAAATAGGTGTTGTTAATGGTTTGGCATATACAAGTTTTGGTGGTTCAACATTAGCTATTGAAGTTACAACATTCCCTGGAAAAGAAGGTTTAAAAATGACAGGGCAACTAAAAGATGTTATGCGTGAATCTGCTCAAATTGCATTAGCTTATGTAAGAGCAAATGCTGCCAAATTTAATATTGACTTTGATTTTGAAAATACAGCAATTCATATTCACGTTCCAGCAGGTGCTATTCCTAAAGATGGCCCAAGTGCCGGAGTAACATTCACAACCTCAATAATTAGTGCCTTATCTAAAAGACCAGTCCCTGCTAATATCGGTATGACTGGAGAAATTACATTGCGCGGAAAAGTTCTACCAATTGGCGGTTTAAAAGAAAAATCATTGGCAGCTGCTCAATTTGGTATTAAAAAGATTTTTATTCCATATGATAATTATAAAAATCTAAAAGATGTTCCTGTTGAAGTTAAAAATGTTGTTGAATTTGTACCAGTTCATTATTATGACGAAATATTTGAACAAATATTTGGCGCCAAGGAAACAAAAAATAATTAA", "aa": "MGGVKDESEIRGHRRTYVGAMPGKIISAIKKAGVSNPVILLDEIDKMSSDFRGDPTSAMLEVLDPEQNVNFQDHYLDLEYDLSKVLFIATANYYDSIPAALIDRVELLELSTYTTKEKVEIAKSHLLPKVYEQNALNPEQFQISDEVLEFLVRYYTRESGVRELKRILDSIARKIVVEILDKKINDSFVVTKEVVIEFLGPIKFDDDENENKAQIGVVNGLAYTSFGGSTLAIEVTTFPGKEGLKMTGQLKDVMRESAQIALAYVRANAAKFNIDFDFENTAIHIHVPAGAIPKDGPSAGVTFTTSIISALSKRPVPANIGMTGEITLRGKVLPIGGLKEKSLAAAQFGIKKIFIPYDNYKNLKDVPVEVKNVVEFVPVHYYDEIFEQIFGAKETKNN", "aa_hexdigest": "ef9dda48f28bbf6dd8f810fe6f6563c5", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A432F9X8", "query_cov": 0.9824120603015075, "subject_cov": 0.8593406593406593, "identity": 0.547, "score": 434.0, "evalue": 1.69e-147, "valid": false, "gene": "lon", "product": "endopeptidase La", "ec_ids": [ "3.4.21.53" ], "uniref50_id": "UniRef50_A0A432F9X8", "go_ids": [ "GO:0004252", "GO:0005524", "GO:0006508", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_A0A432F9X8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A432F9X8" ], "product": "endopeptidase La" }, "genes": [], "id": "GLJLFKCOHC_914", "locus": "GCA013368035_00911" }, { "type": "cds", "sequence": "contig_1", "start": 525187, "stop": 525570, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGTGATGAACTTCTTTCTTTAATTGAAAGTAATGATGTTTTAGCTAGATACAATGAACTAATTAAAAAAGCAAATAAACAAGTTCAATTTGAAAAAATAGAAAACGAAATTAATAGTAATTTACGTGAAGATATGGAAGACCAACAAAGAGATTTCTTCCTAAGAGAAAAAATGCGTCAAATTAGAAAAATGTTGAAGGATGATGAAGGTGGAAAATCTGCTGACGACATTGAAAAAGACAGTACTTTAAAAGAACAATATCCTGAATATGTTTTAGAAGCATTAAGATCTGAACAATCAAGAATGGCTTCAATGATGCCATCTAGCCCAGAAGCTAATATATCTAAAACATATATCGATTTGATCCTTGCATTGCCTTGA", "aa": "MSDELLSLIESNDVLARYNELIKKANKQVQFEKIENEINSNLREDMEDQQRDFFLREKMRQIRKMLKDDEGGKSADDIEKDSTLKEQYPEYVLEALRSEQSRMASMMPSSPEANISKTYIDLILALP", "aa_hexdigest": "9523a146ab3b961fc2e4893bcb42455e", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14864.652299999992, "isoelectric_point": 4.68656826019287 }, "id": "GLJLFKCOHC_915", "locus": "GCA013368035_00912" }, { "type": "cds", "sequence": "contig_1", "start": 525601, "stop": 526134, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTACCTTATATAGCAATAAAAAATCAGATTATTATTCCATATACTTCTGACAAAATTAGAGTTGGAAAAGCTAAATCAGTATCAGCTTTATTAAAGGCTTTTAATGGAAATAAAAATATAGTTATTACATTTTTAAATCCAAATTATAAACAATCAAATGAGGAACATACAATTAATGATATTGCTCCTGTAGGTGTGCTTGCTAATATTACAAACATTAGTGAAGAATCAGGAATTTATCATGTTAATTTAGAAGCAAAAGAACCTGTTAATATTAATGAAATCTCAATAGTTGATGAAAAATCATCAAATAGTGAGGATAATTTTTATGTTAATATTTCGCCTATTCAAGCCAATTCATATGATTCATTTTTACCAAAAATTGAAGAAATAAGACAAAAGTTCTTCTTGCTATCTAAACAAGTTATTAATGAAGGCGTAGGAACAGTTTTATTTCGAGATTTAGAAAAAGAATTTGAAACTAACAAACCTAAACAATCATTAGTTGAATACGAAATTGAATCATGA", "aa": "MKLPYIAIKNQIIIPYTSDKIRVGKAKSVSALLKAFNGNKNIVITFLNPNYKQSNEEHTINDIAPVGVLANITNISEESGIYHVNLEAKEPVNINEISIVDEKSSNSEDNFYVNISPIQANSYDSFLPKIEEIRQKFFLLSKQVINEGVGTVLFRDLEKEFETNKPKQSLVEYEIES", "aa_hexdigest": "7c233355f1bfbfb6303737569e165024", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20037.481800000005, "isoelectric_point": 5.176236152648924 }, "id": "GLJLFKCOHC_916", "locus": "GCA013368035_00913" }, { "type": "cds", "sequence": "contig_1", "start": 526201, "stop": 526989, "strand": "-", "frame": 2, "gene": "grpE", "product": "Protein GrpE", "db_xrefs": [ "COG:COG0576", "COG:O", "KEGG:K03687", "RefSeq:WP_175949931.1", "SO:0001217", "UniParc:UPI00158B9CA7", "UniRef:UniRef100_UPI00158B9CA7", "UniRef:UniRef50_D1J8J6", "UniRef:UniRef90_D1J8J6" ], "nt": "ATGAGTCAAAATATTTCAAAATATGATTATATAGAGTGCATTAGAAAAATTAGCAAAGATGAAAAAATTGAATATAAGGGATTTGTTGGCTTTGATTTAGATAAAAATATTGAAAAAACTATCTTGGAAGGAAAGCAAAACGAAAAAGGTGAGTTAGTTTTAAAAATCGATAATGAAATTTTTAAGGTAATAATTGTTGATTTTCAAAAAACAAGTCCTAAGTATTTAGAAGTTTTTGCTAAAAATCAAGAATTGAATGAAAATATTAAAAAATTACAACTAAATTTTTTGGAATTAGGCGAACTTAATGAAAAAATAAAACAAGAAAAGACACAACAAGAAATTTTGTTTAAAAATCAAGTAATCGAATTGGAAGCAAAAGCACAATCAAAAATAAATGAACATCGCCAAAAAAATGATGAGCATTTATTGCAACAAAAAACAGAATTAAAAAAATATGCCCTACAAGACTTTTTAGAAGAGTTCATTAAGATATATACAAAATATGACAGTGCTTTAAATTTTGCAAAAAAATCTGATAATATCGCTGTTAATAATTTTGCAAAAGGCTTTGATATGCTTAAGAATGATTTTGAAAATTTAATGTTAGATAACGGAATAAAAATAATTGAACCAAAAGTAGGAGATTTATTCGACCCTGAATGTCAACAAATTACTGAAAGCATTGAATCAAAAGAACCTTCTGGCACAATTCTTGAAGTAAAGAGCAATGGTTATTCATTATTTAACAGAATTTTAAAACCTGCCTCAGTAATAATTAGCAAATAA", "aa": "MSQNISKYDYIECIRKISKDEKIEYKGFVGFDLDKNIEKTILEGKQNEKGELVLKIDNEIFKVIIVDFQKTSPKYLEVFAKNQELNENIKKLQLNFLELGELNEKIKQEKTQQEILFKNQVIELEAKAQSKINEHRQKNDEHLLQQKTELKKYALQDFLEEFIKIYTKYDSALNFAKKSDNIAVNNFAKGFDMLKNDFENLMLDNGIKIIEPKVGDLFDPECQQITESIESKEPSGTILEVKSNGYSLFNRILKPASVIISK", "aa_hexdigest": "a2fc31267649be2600d3a126c858132c", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158B9CA7", "ncbi_nrp_id": "WP_175949931.1", "uniref100_id": "UniRef100_UPI00158B9CA7", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B9CA7", "RefSeq:WP_175949931.1", "UniRef:UniRef100_UPI00158B9CA7" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B9CA7", "uniref90_id": "UniRef90_D1J8J6" }, "psc": { "uniref90_id": "UniRef90_D1J8J6", "gene": "GrpE", "product": "Protein GrpE", "uniref50_id": "UniRef50_D1J8J6", "cog_id": "COG0576", "cog_category": "O", "kegg_orthology_id": "K03687" }, "pscc": { "uniref50_id": "UniRef50_D1J8J6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J6" ], "product": "Protein GrpE" }, "genes": [ "grpE" ], "id": "GLJLFKCOHC_917", "locus": "GCA013368035_00914" }, { "type": "cds", "sequence": "contig_1", "start": 527209, "stop": 528042, "strand": "-", "frame": 2, "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "db_xrefs": [ "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355", "SO:0001217", "UniRef:UniRef50_B3PN01", "UniRef:UniRef90_D1J8J7" ], "nt": "ATGGAGCAAAATTCTTCTGACACAAACACAAATAAGTCTTTAATCATATGCAATAATTATGATAAAAAACTAATTAAATATTTTAGATACATTGTTGAAATGTACATTAAAACAGGTGAACCAGTAGGATCAAAGAGACTAGTTGAAAAGTTCAAATTATCTTGTAGCTCTGCAACCATAAGAAACGCAATGGCAGAATTAGAAAAATTAGGTCTTTTGGAAAAAAGACATGTTTCAGGAGGAAGAATTCCTTCTTCAAAAGGTCTTGAATACTATACAAAAAATTTACTTTATAATGCTAAAAAATATTTCAATGAAAAACTTGAAGATCTTTTATCTAAGCGTAGGTTAAGCATCAACACAACATTAGAAGAAGCAGCAAACGTTGTTAGCCAAATGGCAGGTTTTGCCGTAGTTGCAACCTCAAATAATGAATCTGAATTATTGAAAAGTATTCAATTGACACCGCTTGATGAAGAATCTGCAGTTGTTGTTATTGTTACAAGCTCGGGAAATGTTCAAAACAAAATGTTTAAGTTTGAAAGAGGGATTGAATTAAATGATTTAAGGATTGCAATTAGGTTATTTAAAGAAAGATTGATTGATACTCCATTAATTAATCTTGCTAAAAAAACTGAATCATTAGCCCCAATATTATCTACACAAGTTAAACATTTTGAATTAATAATGCAGAAATTTATCAAAAATATCTTTTGTTTTGAAGAAAAATTGGAGAATAAATCATTTAATAAAAATGTAATAATATTATCAGAACAAATAAGCAGAGAAGAAATCGCTAAAACATTAGAATTAATTGAAAACCACTCAGTTTGA", "aa": "MEQNSSDTNTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKRHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFERGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSV", "aa_hexdigest": "18b413e457e8778b1b0760aaea8fa491", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "hrcA" ], "truncated": "5-prime", "pseudogene": { "start": 526990, "stop": 528042, "observations": { "mutation": [ 527211 ], "stop": [ 527211 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J7", "query_cov": 1.0, "subject_cov": 0.7914285714285715, "identity": 0.993, "score": 519.0, "evalue": 1.46e-184, "gene_start": 1, "gene_end": 277, "reference_start": 1, "reference_end": 277, "reference_sequence": "MEQNSSDTNTNKSLIICNNYDKKLIKYFRYIVEMYIKTGEPVGSKRLVEKFKLSCSSATIRNAMAELEKLGLLEKKHVSGGRIPSSKGLEYYTKNLLYNAKKYFNEKLEDLLSKRRLSINTTLEEAANVVSQMAGFAVVATSNNESELLKSIQLTPLDEESAVVVIVTSSGNVQNKMFKFESGIELNDLRIAIRLFKERLIDTPLINLAKKTESLAPILSTQVKHFELIMQKFIKNIFCFEEKLENKSFNKNVIILSEQISREEIAKTLELIENHSVWEAIENNLDEEKNIKLDFSHPNLNIISKRIQFINHKNIKEISILGPKNLDVEESLEAINLFEGIIKKEEEKGE" }, "paralog": false, "identity": 0.9914285714285714, "score": 666.0, "evalue": 7.04e-245, "description": "Internal stop codon at 527211. Nonsense mutation around 527211.", "psc": { "uniref90_id": "UniRef90_D1J8J7", "gene": "hrcA", "product": "heat-inducible transcriptional repressor HrcA", "uniref50_id": "UniRef50_B3PN01", "cog_id": "COG1420", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0006355" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J7", "UniRef:UniRef50_B3PN01", "COG:COG1420", "COG:K", "GO:0003677", "GO:0006355" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN01", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN01" ], "product": "Heat-inducible transcription repressor HrcA" } }, "id": "GLJLFKCOHC_918", "locus": "GCA013368035_00915" }, { "type": "tRNA", "sequence": "contig_1", "start": 528169, "stop": 528245, "strand": "-", "gene": "trnR", "product": "tRNA-Arg(acg)", "amino_acid": "Arg", "anti_codon": "acg", "score": 76.1, "nt": "GCGCCCGTAGCTCAATTGGACAGAGTGTTTGGTTACGGCCCAAAAGGTTGCGGGTTCGACTCCTGCCGGGCGCGCCA", "db_xrefs": [ "SO:0001036" ], "anti_codon_pos": [ 528209, 528211 ], "id": "GLJLFKCOHC_25", "locus": "GCA013368035_00916" }, { "type": "tRNA", "sequence": "contig_1", "start": 528248, "stop": 528322, "strand": "-", "gene": "trnC", "product": "tRNA-Cys(gca)", "amino_acid": "Cys", "anti_codon": "gca", "score": 67.3, "nt": "GCCACCATAGCCAAACGGCCAAGGCATAGGTCTGCAACACCTTGATTACCGGTTCGAATCCGGTTGGTGGCTCCA", "db_xrefs": [ "SO:0000258" ], "anti_codon_pos": [ 528297, 528299 ], "id": "GLJLFKCOHC_24", "locus": "GCA013368035_00917" }, { "type": "cds", "sequence": "contig_1", "start": 528524, "stop": 529045, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J8", "UniRef:UniRef90_D1J8J8" ], "nt": "TTGAACACAAACCAATCATTAGAAAATTTTGCGCCTGAATTAAAAAATGAAATCAAAGATATTACTTCGATGAAATCAAATCAGGGATTATTGCTTATTGATCAAAATCTTGTTAATGCAAGAAATATTTTAAAATTTTTAGCATTTTTATTTGGCACTAAGAAAAACAAAAGCGTAGCATTTGTTGACAACAATAATTTGTTTGAATTCATGTCTAATAATTTAAAAAACTCATCAGAAATTAATTCAATTGTTCAAAACTTAATTTCTGTTGATTATTTATTCATAGATCGCTTTGCAATCGGAGTCAAACCGGAATGGTATATTACAAACATATTTAATATATTGAGCGAACGTGAACAAAAAAATAAAATCACTTTTATGAGCTCACCTATAGATTTTACTTCTTCGAAGCAACATATAATATTTAATAAAATGCCTGGTAATGATGGCATAACTCGGATTGAATCATTGCTTAAATTGACAGTCTTAAGATTAATGAAAAAGTTCATTTCGTTCTAA", "aa": "MNTNQSLENFAPELKNEIKDITSMKSNQGLLLIDQNLVNARNILKFLAFLFGTKKNKSVAFVDNNNLFEFMSNNLKNSSEINSIVQNLISVDYLFIDRFAIGVKPEWYITNIFNILSEREQKNKITFMSSPIDFTSSKQHIIFNKMPGNDGITRIESLLKLTVLRLMKKFISF", "aa_hexdigest": "5016187b76dba128f59bc68de8169264", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 528524, "stop": 529345, "observations": { "mutation": [ 529096, 529129 ], "stop": [ 529096, 529129 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8J8", "query_cov": 1.0, "subject_cov": 0.5766666666666667, "identity": 0.983, "score": 328.0, "evalue": 1.7e-111, "gene_start": 1, "gene_end": 173, "reference_start": 128, "reference_end": 300, "reference_sequence": "MENKIFDELFKNSLLKNIDLEAEKAKILADPNVKAIIEKLNLNSKQIQKGMNYLQKYYSYIETHKQLPIWTLFVDDNGILQIDLSNDGFFRKQKEMNNFWLTNITSMDNEWIYYFNNAKAKKPQYIVLNTNQSLEKFAPELKNEIKDITSMKSNQGLLLIDQNLVNARNILKFLAFLFGTKKNKSVAFVDNNNLFEVMSNNLKNSSEINSIVQNLISVDYLFIDRFAIGVKPEWYITNIFNILSEREQKNKITFMSSPIDFTSSKQHIIFNKMPGNDGITRIESLLKLTVLRLMKKFISF" }, "paralog": false, "identity": 0.9743589743589743, "score": 523.0, "evalue": 8.56e-191, "description": "Internal stop codon at 529096, 529129. Nonsense mutation around 529096, 529129.", "psc": { "uniref90_id": "UniRef90_D1J8J8", "uniref50_id": "UniRef50_D1J8J8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8J8", "UniRef:UniRef50_D1J8J8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8J8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J8" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 19965.930000000004, "isoelectric_point": 9.454498100280762 }, "id": "GLJLFKCOHC_919", "locus": "GCA013368035_00918" }, { "type": "cds", "sequence": "contig_1", "start": 529127, "stop": 529426, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAACAAAATATTTGATGAATTGTTTAAAAATTCTCCTTTGAAAAATATTGATTTAGAGGCTGAAAAAACAAAAATTCTAGCCGATCCAAATGTAAAAGCAATTATTGAAAAATTGAATTTGAATTCTAAACAAATTCAAAAAGGAATGAATTACTTGCAAAAATATTATAGCTATATTGAAATGCATAAACAACTTCCAACATGGACCCTCTTTGTTGATGATAGTGGAATTTTGCAAATTGATTTGAGCAACGATGGTTTTTTTAGAAAGCAAAAAGAAATGAATAACTTTTGA", "aa": "MENKIFDELFKNSPLKNIDLEAEKTKILADPNVKAIIEKLNLNSKQIQKGMNYLQKYYSYIEMHKQLPTWTLFVDDSGILQIDLSNDGFFRKQKEMNNF", "aa_hexdigest": "cf63572f74635693f2a4c54feffd2361", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11719.366099999992, "isoelectric_point": 6.571974372863769 }, "id": "GLJLFKCOHC_920", "locus": "GCA013368035_00919" }, { "type": "cds", "sequence": "contig_1", "start": 529455, "stop": 530351, "strand": "-", "frame": 3, "gene": "dnaB2", "product": "DnaD domain-containing protein", "db_xrefs": [ "COG:COG3611", "COG:L", "KEGG:K03346", "SO:0001217", "UniRef:UniRef50_D1J8J9", "UniRef:UniRef90_D1J8J9" ], "nt": "ATGGTTGAAAATTTTTTTATTGAAAGCGAAAGGGAAATTACACAAAATGATTTTAAAAATCTAGTAATTTTTTATGCTCCGATTTTAGGCCATAGTAGCATATTTCTATATCAATTTTTATATAATTTAAAAAATATCTATGCAAAAAATAAACTTTTTAATTTTTCTGAAATTAAAGATATTTTATTATTAAATGAACAGGAATTTGAAGAATGTCGTAGCAAACTAGAAGCATTAAGCTTACTAAAAAGTTATTTAAATAAAGATGGTGTTTATATTTTTAGTTTAAATGCTCCATTGAATGCAAATGAAATAAGCAATAATAAAATTATTTCTAATATGTTAATTAAATTAATCACTAAATCAAAATATGAAGAATTATTAAAATTAAATGCAAATTATCAATTTGATAAAACAACAATTAATGATGTTTCTAAAAAATTTTATGAAGTATTTAATTTAGAAAACGTAATTGATTTAAATATAAAAAATGAACAATTAAATTTTAATTCAATTACCCTAGATGAATTAAAGGATAAGATAGAACCCGAACAATTTATTAGTCAATATACAAAAACAAATTTAAGTTTTAGCCAAAATGAAATGATTAGAAAATTGAAAAGAATGAAATTCAATAATTTTTCAATAAATGCCTTCATTTATTATTCAATTAAAATTAACCATTCAGTTGTATGCAAATATATTGAAAAAATAGCCGAAGATTTTAGTCATAGAAATCTATACGATGCCGAAAGAATTGATCAAGAATTAGAAAATGTTTATTTAATAAAGCAAAACGCAAATAAAGAGTTAAAAAATATCAAAAAAAATGAAGATGAATCGGTTACTAGAGAAGATTTAAATTGTTTTTTAGATCTTGCTAATGAACACGATTGA", "aa": "MVENFFIESEREITQNDFKNLVIFYAPILGHSSIFLYQFLYNLKNIYAKNKLFNFSEIKDILLLNEQEFEECRSKLEALSLLKSYLNKDGVYIFSLNAPLNANEISNNKIISNMLIKLITKSKYEELLKLNANYQFDKTTINDVSKKFYEVFNLENVIDLNIKNEQLNFNSITLDELKDKIEPEQFISQYTKTNLSFSQNEMIRKLKRMKFNNFSINAFIYYSIKINHSVVCKYIEKIAEDFSHRNLYDAERIDQELENVYLIKQNANKELKNIKKNEDESVTREDLNCFLDLANEHD", "aa_hexdigest": "dcf8f2cb9a8d5f07cc5c644d02766ac2", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8J9", "query_cov": 1.0, "subject_cov": 0.9900332225913622, "identity": 0.993, "score": 567.0, "evalue": 5.11e-204, "valid": true, "gene": "DnaB2", "product": "DnaD domain-containing protein", "uniref50_id": "UniRef50_D1J8J9", "cog_id": "COG3611", "cog_category": "L", "kegg_orthology_id": "K03346" }, "pscc": { "uniref50_id": "UniRef50_D1J8J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8J9" ], "product": "DnaD domain-containing protein" }, "genes": [ "dnaB2", "dnaD" ], "id": "GLJLFKCOHC_921", "locus": "GCA013368035_00920" }, { "type": "cds", "sequence": "contig_1", "start": 530665, "stop": 531015, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAATTAATTAACCTTCACTTAAACACTATATTTTCTTTTTTAGAAAGCACGATAACCATTAATGATTTTATTCAAGAATTATTAAAGAATAATATAAAATATTTTGGCATTACAGAACATTGTAATTTTTATAGCATGCCATATTTTTTAAATGAAGCTAGAAAATATGATTTAAAACCTATTTTTGGTCTTGATGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTACGGAAAAAAGGGCTATATAGATTTAGATGAAATTAGAGATTATTCTAATTTAATATGA", "aa": "MKLINLHLNTIFSFLESTITINDFIQELLKNNIKYFGITEHCNFYSMPYFLNEARKYDLKPIFGLDVNVKIENNLYRFIVYCQNKESFMNLKMLSLNYGKKGYIDLDEIRDYSNLI", "aa_hexdigest": "54c9ef2a5e944749ecab7a4e93e71af6", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13900.982699999984, "isoelectric_point": 6.715492820739747 }, "id": "GLJLFKCOHC_922", "locus": "GCA013368035_00921" }, { "type": "cds", "sequence": "contig_1", "start": 531199, "stop": 531456, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGAATATTCCAAATAATAAAATATTTGAAAGCATTTACGAAGAATTTAAGGTTGATATTGAAACAAAAAATAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAATGATTTTAAAATGCCTAAATTTAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTCGATAAGTTTTCATGA", "aa": "MSNIPNNKIFESIYEEFKVDIETKNNSKLEELIERTNNFAKKLYFEIETNDFKMPKFKNDKNIASNEYLKLLLFKSIREKFDKFS", "aa_hexdigest": "2de75fdabd67f7895e739d25e44fbfef", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10295.663599999987, "isoelectric_point": 7.867093086242677 }, "id": "GLJLFKCOHC_923", "locus": "GCA013368035_00922" }, { "type": "cds", "sequence": "contig_1", "start": 531700, "stop": 533655, "strand": "+", "frame": 1, "gene": null, "product": "DNA polymerase III subunit alpha", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023586AB7" ], "nt": "ATGCCTGACATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTCGTGGCAAATATTGTTACTTTTTCTTCACTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTTTTGGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCATTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACACATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCGATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGCATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAGACATTGACGACTATTTCAAATATAATTGATCTAATTTATCAAAATCAAAATCTTAAAATTAACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAATTATTGAGCTCTGGTCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGGCAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCGGGTCCAATGAAAAATGTTTCTGTTTATGCAAAAAGAAAATTTGGACTAGAATCGATACCAAAAATAAATAATGAATATGATAAAATCCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATCATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCGGACAATCTAAGAAGGATTATTTCTAAGAAAAAAGTAAATGAAATGGAAGATATTAAATCCATTTTTATTGAAAGAGCAATGAAAAATGGCTATGAAAAATCTTTGGCTACAAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTCAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCATTACCCTTTAGAATTTTATACGTCATGTATAAGCTCGGCACACGGCTCCCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGTGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAGGCTATAATTAAAAATAAAAAAATTATATTGCCGCTTACACTAATTAAAGGCCTTGGACCTGAAATTGCTAATTCTATTGTTGAAAATAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTATTAATAAAGGCAAACACTCTTAGATCATTTAATTATAATCAAAACACCTTTTTGAATGAGATTCAAAAAAATAATACTGATACAATGTTATTTATAAAAATGTTTAAAAATAACATTGAAGGTGTTAGCCCTGATATAATAAACTCTTATAAACCAAGTGAAATTCTTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTATTAGGTCAAGTTTATAATTTTAATTCAAACGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATCGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATATTAAGGGAAGAACTATCAAATTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAA", "aa": "MPDIDIDVQDNKRNDVIEYLLKKYGHEFVANIVTFSSLGKKSAIRDILRINNIAPTLINEISKNIENDEQDLLVEYNENRNLQLSLAKLNPNDESFSTRILNQAARISGFYRQIGTHAAGLVISDQPVISIAPVQYNSENFLQTQISMEYLEDFGLVKMDILGLKTLTTISNIIDLIYQNQNLKINLKDIDLTDQKTLELLSSGHTRGVFQVESPIMIKALRQVKVNNFEDIVAIISLNRPGPMKNVSVYAKRKFGLESIPKINNEYDKILDSTYGIIVYQEQIMKIAQVVAKMTFIEADNLRRIISKKKVNEMEDIKSIFIERAMKNGYEKSLATNIFNSIEKFASYGFNRSHAVSYSIITFQMAFLKAHYPLEFYTSCISSAHGSQDTIAKFVNEASECGVEIVSPDINLSESEAIIKNKKIILPLTLIKGLGPEIANSIVENRSKYGPYKNFLDFLLKISSSQSIGKSTIELLIKANTLRSFNYNQNTFLNEIQKNNTDTMLFIKMFKNNIEGVSPDIINSYKPSEILNQDIMFEKKNEIELLGQVYNFNSNASNGMPGQTSFIDMHVGNEYLAIVNCTNVKQKISKKGNNYFELDLEANMQKIRAFYFTSNQEILNIKGRTIKLTLEKRNENIFYLRSIKEVLNEER", "aa_hexdigest": "1ecbdf1c3b2bd3503a1d9861cc9793d3", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0023586AB7", "query_cov": 0.9815668202764977, "subject_cov": 0.8753424657534247, "identity": 0.502, "score": 586.0, "evalue": 3.61e-199, "valid": false, "product": "DNA polymerase III subunit alpha", "uniref50_id": "UniRef50_UPI0023586AB7" }, "pscc": { "uniref50_id": "UniRef50_UPI0023586AB7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023586AB7" ], "product": "DNA polymerase III subunit alpha" }, "genes": [], "id": "GLJLFKCOHC_924", "locus": "GCA013368035_00923" }, { "type": "cds", "sequence": "contig_1", "start": 533642, "stop": 534535, "strand": "+", "frame": 2, "gene": "exoIX", "product": "DNA polymerase I", "db_xrefs": [ "COG:COG0258", "COG:L", "RefSeq:WP_020002546.1", "SO:0001217", "UniParc:UPI0003651CA3", "UniRef:UniRef100_A0A2K9YTX0", "UniRef:UniRef50_A0A318U4A1", "UniRef:UniRef90_D1J8K1" ], "nt": "ATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCCTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCAGAAGGATTTGAAACCAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATCTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCGCCAGAAAGTTTTTATCTACAGCTTGATTTAATTCAAGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAAGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAATTAATAGATGAACAAACAGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTACCAAATCAAGTTATTGATTATAAAGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGTCCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACATTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAGAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTTTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAA", "aa": "MKKDNEQLLIIDGTFLAYRSYYATSYNATQILTTSEGFETNAIVAFFKTLFLLLKKLNPKYIYIAFDSHVKTFRHELFPSYKDGRKKAPESFYLQLDLIQEILTNINIQNQYYNGFEADDIIAKVKNKFNLNTIIFSGDQDLNQLIDEQTAIIKKDKTGSFFFLNSKNFSEYYDFLPNQVIDYKALVGDNSDNFKGVVGIGPKSAKTLLEDYKTIENIYNNIEKIKPSIAKKLMEYKENTFLDKYLATLRIDFDLQLPELENLAISNIAISNSAKQIIDKFELYSLENSLKNLINKK", "aa_hexdigest": "a6b2523fe6605cc4d24d3bfc903ae80d", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI0003651CA3", "ncbi_nrp_id": "WP_020002546.1", "uniref100_id": "UniRef100_A0A2K9YTX0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0003651CA3", "RefSeq:WP_020002546.1", "UniRef:UniRef100_A0A2K9YTX0" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTX0", "uniref90_id": "UniRef90_D1J8K1" }, "psc": { "uniref90_id": "UniRef90_D1J8K1", "gene": "ExoIX", "product": "DNA polymerase I", "uniref50_id": "UniRef50_A0A318U4A1", "cog_id": "COG0258", "cog_category": "L" }, "pscc": { "uniref50_id": "UniRef50_A0A318U4A1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318U4A1" ], "product": "5'-3' exonuclease, exo domain of DNA polymerase I" }, "genes": [ "exoIX" ], "id": "GLJLFKCOHC_925", "locus": "GCA013368035_00924" }, { "type": "cds", "sequence": "contig_1", "start": 534562, "stop": 534933, "strand": "-", "frame": 2, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "TTGCCAGCAACAACAAAAATTTTAACTTTTGGGAAGTCAAAAAAAGGAATAATAAAACTTAGAGATGAATTATTAAATGCTTTTCATGATATTTCAACACATATTGTATCAAAAGGATATTCAATTGAAATTAGCGATATTAATGCTAATAAAGGAATTGGCGATCGATTTGTATGTGATTTGTTAAATATTGATTATAAAGAAGCAGTTCACTTAGGTGATTCGGGCAATGATGTTGTTACTAAAAAATATTTAGGTGGCTTTGTAGCGATGAAAAATTCTACAAAGAACATAAAACAAAAAGCAGATTATGTAGGATATTCATATAAAAAATCTGGAGTAGCAAAAACATTGCAAGAACTTCAAAAATAA", "aa": "MPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK", "aa_hexdigest": "6f3e8c859900137c075ef2f9eb7bf287", "start_type": "TTG", "rbs_motif": null, "genes": [ "cof" ], "truncated": "3-prime", "pseudogene": { "start": 534562, "stop": 535233, "observations": { "mutation": [ 534978 ], "stop": [ 534978 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.458955223880597, "identity": 0.992, "score": 241.0, "evalue": 1.22e-78, "gene_start": 1, "gene_end": 123, "reference_start": 146, "reference_end": 268, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9955156950672646, "score": 437.0, "evalue": 3.42e-158, "description": "Internal stop codon at 534978. Nonsense mutation around 534978.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "GLJLFKCOHC_926", "locus": "GCA013368035_00925" }, { "type": "cds", "sequence": "contig_1", "start": 534976, "stop": 535368, "strand": "-", "frame": 2, "gene": "cof", "product": "HAD family phosphatase", "db_xrefs": [ "COG:COG0561", "COG:HR", "SO:0001217", "UniRef:UniRef50_A0A318UA29", "UniRef:UniRef90_D1J8K2" ], "nt": "ATGATTGATTTTAAACCTAAGGCATATTTTTTGGATATGGATGGCACATATCTAGATTTGCCTCGCAAAAAACAAATTATGATAAGCGAAAAAAATGTTGAGGTTGCTAAGGACTTCAATAATAGAGGAATCCCTGTTATTTTGTCAACAGGTAGAGGAAATTCAAAGTTTGTTTTGGATTTAACTAATAAAATAAATGCACCGTTTGCTGTTTGCCAAAATGGTGGAGTAATTGTAGATAAAAATAATAATGTTTTGAAACGCCATGAAATTAACCCTGATACATTGAAATCAATAATTGAAATTTTAATTGCACAAAAAATGTTTTTTATTTTTAATAGTGGCGATACAATATATGGAACAAAAACAAAATTTAGAATCGCTAGAGCTTGA", "aa": "MIDFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARA", "aa_hexdigest": "a1866e85facaa78a9fbe437420c7ae80", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "cof" ], "truncated": "5-prime", "pseudogene": { "start": 534673, "stop": 535368, "observations": { "mutation": [ 534978 ], "stop": [ 534978 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K2", "query_cov": 1.0, "subject_cov": 0.48507462686567165, "identity": 0.992, "score": 260.0, "evalue": 5.83e-86, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MINFKPKAYFLDMDGTYLDLPRKKQIMISEKNVEVAKDFNNRGIPVILSTGRGNSKFVLDLTNKINAPFAVCQNGGVIVDKNNNVLKRHEINPDTLKSIIEILIAQKMFFIFNSGDTIYGTKTKFRIARAWLKKLNKVTYDEIQSLPATTKILTFGKSKKGIIKLRDELLNAFHDISTHIVSKGYSIEISDINANKGIGDRFVCDLLNIDYKEAVHLGDSGNDVVTKKYLGGFVAMKNSTKNIKQKADYVGYSYKKSGVAKTLQELQK" }, "paralog": false, "identity": 0.9913419913419913, "score": 454.0, "evalue": 6.33e-165, "description": "Internal stop codon at 534978. Nonsense mutation around 534978.", "psc": { "uniref90_id": "UniRef90_D1J8K2", "gene": "Cof", "product": "HAD family phosphatase", "uniref50_id": "UniRef50_A0A318UA29", "cog_id": "COG0561", "cog_category": "HR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K2", "UniRef:UniRef50_A0A318UA29", "COG:COG0561", "COG:HR" ] }, "pscc": { "uniref50_id": "UniRef50_A0A318UA29", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A318UA29" ], "product": "COF family HAD hydrolase protein" } }, "id": "GLJLFKCOHC_927", "locus": "GCA013368035_00926" }, { "type": "cds", "sequence": "contig_1", "start": 535376, "stop": 535582, "strand": "-", "frame": 1, "gene": null, "product": "Thioredoxin", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "nt": "ATGATTGGCCCAGAATTAGAAAAATTAGCAGAAGAAAATTCAGATGTTAATGTTTTTGATTTGAACGTAGATAAATTCATTGATTTAGCAAGAGAAATGAATGTATCTCATATTCCATCGTTATTTTATTATAGAGACGGTAAGTTAGTTTCTCAAAGTGTAGGATACTTGCCAGTTGAAGAAATTTTAAACAATTTAAATAAGTAG", "aa": "MIGPELEKLAEENSDVNVFDLNVDKFIDLAREMNVSHIPSLFYYRDGKLVSQSVGYLPVEEILNNLNK", "aa_hexdigest": "d6a9e1f63bb324bb8081203c85a066ad", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P2T3", "query_cov": 0.8382352941176471, "subject_cov": 0.9193548387096774, "identity": 0.526, "score": 72.8, "evalue": 1.09e-15, "valid": false, "product": "Thioredoxin", "uniref50_id": "UniRef50_A0A3B0P2T3" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P2T3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P2T3" ], "product": "Thioredoxin" }, "genes": [], "id": "GLJLFKCOHC_928", "locus": "GCA013368035_00927" }, { "type": "cds", "sequence": "contig_1", "start": 535598, "stop": 535762, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATACTTGTTTTTGAACTAATAGAAAAACAAAAAGATTTGTATTATTTGCAAGAGCATATTAAAGGAGAAAATATTATGTATAAAACTATTGAATCAGTTGAAGAATTCAAAAAGAATGAATATTTAGAAGGAAAATCAATTGTTGCATTTAGAGCAGTTTGA", "aa": "MILVFELIEKQKDLYYLQEHIKGENIMYKTIESVEEFKKNEYLEGKSIVAFRAV", "aa_hexdigest": "e3c34a065d1b30a44f763fd2991c67c8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6499.5041, "isoelectric_point": 5.297132682800293 }, "id": "GLJLFKCOHC_929", "locus": "GCA013368035_00928" }, { "type": "cds", "sequence": "contig_1", "start": 535746, "stop": 535868, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09180.16" ], "nt": "GTGTATGCTGCATTTGATGGTAACGGTGATGATGAAGTAAAAATTAAAGAAGAAACAGGAGCATCAACAAGGTGTATTCCATTTAAAGAACCAATAAAAGTTTCAAATGATACTTGTTTTTGA", "aa": "MYAAFDGNGDDEVKIKEETGASTRCIPFKEPIKVSNDTCF", "aa_hexdigest": "79c1090abc86c7c2c3a94b405e703492", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09180.16", "name": "ProRS-C_1", "length": 40, "aa_cov": 0.95, "hmm_cov": 1.0, "evalue": 6.21739761228377e-11, "score": 37.764034271240234, "start": 3, "stop": 40 } ], "seq_stats": { "molecular_weight": 4427.896500000001, "isoelectric_point": 4.507070732116699 }, "id": "GLJLFKCOHC_930", "locus": "GCA013368035_00929" }, { "type": "cds", "sequence": "contig_1", "start": 535869, "stop": 536321, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03129.25" ], "nt": "ATGACTCATGGTGATGATCGAGGAATAATCATTCCACCCAAAATAGCAAGGACTCAAGTTGATATATTAGAAATTTTTAATTCAAAGGATGAACGAGTTCACAAACTTGCTCTTGAATTAAAAGATGCTTTATTAGCAAAAAATATTGTTGCAAACATAGATTCTTCAAACAAGGGTCCAGGTTTTAAAGCAGCAAATTCAGAAATTCATGGTACACCATTGAGAATAGAAATCGGTCCAAAGGATATTGAAAATAATAGTGTATTATTCGTTAGACGTGATAACCTAGAAAAAATCACTTCAACAATTGCTGAAGCAGCAAATATTGCCAGTCTCTTATTACAAAAAATACAAGACAATTTATTTGAAAGTGCTAAACAACGCTTACAAAACAATATTGTAACTGTTAATAATTATCAAGAATTTAAGGAAGCAATTGCTGCGGGTCACTGA", "aa": "MTHGDDRGIIIPPKIARTQVDILEIFNSKDERVHKLALELKDALLAKNIVANIDSSNKGPGFKAANSEIHGTPLRIEIGPKDIENNSVLFVRRDNLEKITSTIAEAANIASLLLQKIQDNLFESAKQRLQNNIVTVNNYQEFKEAIAAGH", "aa_hexdigest": "842bdaeaeb3bd23c7e6c122822dece20", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03129.25", "name": "HGTP_anticodon", "length": 82, "aa_cov": 0.5466666666666666, "hmm_cov": 1.0, "evalue": 8.052335320013221e-16, "score": 53.448184967041016, "start": 27, "stop": 108 } ], "seq_stats": { "molecular_weight": 16619.735999999986, "isoelectric_point": 6.423908805847168 }, "id": "GLJLFKCOHC_931", "locus": "GCA013368035_00930" }, { "type": "cds", "sequence": "contig_1", "start": 536355, "stop": 536600, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCACAAGGTTTATAAAGATGTTATTGAAGAAAATTTAGCAATTCCAGTAGTAGAAGGCTGCAAAACGGATCATGAAAAATTTGCTGGCGCTGATATAACATACACCTTAGAATCAATGATGAAGGACGGGAAGGCACTTCAATCAGGTACTAGCCACTATTTAGGACAAAATTTTACTAAGGCATTTGATATAACATTTAAAAACAATGAAAATAAATTAATCAATCCTTATGGTACCAGTTGA", "aa": "MHKVYKDVIEENLAIPVVEGCKTDHEKFAGADITYTLESMMKDGKALQSGTSHYLGQNFTKAFDITFKNNENKLINPYGTS", "aa_hexdigest": "95952e5e14c27a02029175ccc4162958", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9088.161999999993, "isoelectric_point": 5.837330436706543 }, "id": "GLJLFKCOHC_932", "locus": "GCA013368035_00931" }, { "type": "cds", "sequence": "contig_1", "start": 536721, "stop": 536972, "strand": "-", "frame": 3, "gene": null, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "nt": "ATGGATATTGAATTTAAAAAGAGAGGCGTTCAAAACGTTTATTTACCTCTTTTAATACCAGAAAGCTTATTGCAAAAAGAAAAAAATCATGTCGAAGGATTTAATCCTGAACTTGCAACTGTTACTGAAGTAGGCGGCAAAAAATTGGCAGAAAAGTTTTTTATAAGACCAACTTCAGAAACGCTGTTTGCTGATTTATTCAAAAATGAGGTTTCGTCTTACAATGATTTGCCAATAATTTATAATCAATGA", "aa": "MDIEFKKRGVQNVYLPLLIPESLLQKEKNHVEGFNPELATVTEVGGKKLAEKFFIRPTSETLFADLFKNEVSSYNDLPIIYNQ", "aa_hexdigest": "be7654839c9a3e753250bfb8135032dd", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A661HMY4", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.614, "score": 102.0, "evalue": 4.5e-27, "valid": false, "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein", "uniref50_id": "UniRef50_A0A661HMY4" }, "pscc": { "uniref50_id": "UniRef50_A0A661HMY4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A661HMY4" ], "product": "Aminoacyl-transfer RNA synthetases class-II family profile domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_933", "locus": "GCA013368035_00932" }, { "type": "cds", "sequence": "contig_1", "start": 537191, "stop": 537586, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACGTTGCAATTGACATTGCTTCTTCAAATACCAAAAACCAATTTGTATTTGTAAATCTTGTTCAATTTGACAGCCATTTTGGACATAGAGAAGACCCAATAGGATATTCACAAAATATTTCAAGGCTAGATATTAAATTAACAAAATTGATAAACGCAATGAAATCAGATGACTTGTTAATTATAACTTCTGACCATGGAAATGACCCAACAAAAGGCCCTGATCATACAAGAGAAGCATTGCCATTGACAATATTTTCAAAGAAATTCAAATCACCATGTGTATTTAAAGAACCTTTGAAAGGCTTGGGAACTGCAGGAAATATTGTTGCAAGAAATTTCAATGTTCCAACCGTTCAAACAGGTGAAGATATTTTTGACAAATTAATCTAA", "aa": "MDVAIDIASSNTKNQFVFVNLVQFDSHFGHREDPIGYSQNISRLDIKLTKLINAMKSDDLLIITSDHGNDPTKGPDHTREALPLTIFSKKFKSPCVFKEPLKGLGTAGNIVARNFNVPTVQTGEDIFDKLI", "aa_hexdigest": "efe568986058dda60c7d493c057c6d40", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14543.415699999978, "isoelectric_point": 6.397819709777832 }, "id": "GLJLFKCOHC_934", "locus": "GCA013368035_00933" }, { "type": "cds", "sequence": "contig_1", "start": 537806, "stop": 538108, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATGGGAATTTATACAAAAGTTCCAAACCCACAATTTATTGAAAAAGGTTTCCCTGCTGATTTAGTTGCAGAATTGTCAAAAGCGTTTGATAATAGAAAAATTATTGGAAATAGAAATGCATCAGGTACAGTTATATTGGCCGAATTAGGTTTGCAACATATTGAAAAAGGAGATATGATAATTTACACTTCTCCTGATTCTACATTGCAAATTATTGGGGATGAAAAAACATTGGGTGTTGAAAAACTAACTGAATATGCAAAGAGAGCAAGAGCAATTTGTTCATCAAGACCTGAATGA", "aa": "MMGIYTKVPNPQFIEKGFPADLVAELSKAFDNRKIIGNRNASGTVILAELGLQHIEKGDMIIYTSPDSTLQIIGDEKTLGVEKLTEYAKRARAICSSRPE", "aa_hexdigest": "5d61518090ed1c2deb07abf8c68a2cfe", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11024.595599999986, "isoelectric_point": 6.585217857360839 }, "id": "GLJLFKCOHC_935", "locus": "GCA013368035_00934" }, { "type": "cds", "sequence": "contig_1", "start": 538238, "stop": 538387, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAACAAAAATTTAATAGAATATTTTTTATAGTTACAGATGGTCTTGGCATTGGATATGAACCACGCCAAGCTGAATTTCATGATGAAGGTGCAAATAGCATGTTGCATGCATCAGAAGCATTGCCACTAGAGATTCCTACATGA", "aa": "MQEQKFNRIFFIVTDGLGIGYEPRQAEFHDEGANSMLHASEALPLEIPT", "aa_hexdigest": "4eec1535153f3fde8ad1d1f95641cfd4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5549.166199999999, "isoelectric_point": 4.583291816711426 }, "id": "GLJLFKCOHC_936", "locus": "GCA013368035_00935" }, { "type": "cds", "sequence": "contig_1", "start": 538679, "stop": 539929, "strand": "+", "frame": 2, "gene": "pyk", "product": "pyruvate kinase", "db_xrefs": [ "COG:COG0469", "COG:G", "EC:2.7.1.40", "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955", "KEGG:K00873", "SO:0001217", "UniRef:UniRef50_L0RUB4", "UniRef:UniRef90_D1J8K6" ], "nt": "ATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGTGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACATTCCAATCTCATTAATGCTTGACACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAAAACAGATGAAAATTCATACAAGAATTTTTTAGGTACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAAAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAGGATGTTAAAAAATTTGAAATTATTGTTGAAACAGTTAATGAACATTTCTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAGGATATTGACGATATTATATGGGGTGCAAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAACGCAAATGGTGGAGAAAATATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATTAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTTGCAAGAGGAGACCTTGGACTTGAAATTCCTTACTACGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTTGGCAAGGAAGTAATAGTTGCAACTCAAATGCTAGATTCAATGGAAAACAACCCTATTCCAACAAGAGCGGAAGTTACTGACGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCTGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTGGCAAATCATCCTACTTCAAGAATGTTAATTGCTTATGAAGTTGCAAAAATCCTTCAAAGCGGTGAATACAAATTTGCTATTATATTAAGTCACACCGGAAAATTATTAAAAGAAGTTGCTAAATACAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTGGTTTGA", "aa": "MKFNELRTKIVATIGPSSDNYDTMKSLIEAGVTTIRANFSHGGPETHVNKFEIARQISKELNIPISLMLDTKGPEIRVGKMENDGVTIPANHILKIKTDENSYKNFLGTQDQITVSYRMDNDLKIGDKVLIDDGKLVTTVKDVKKFEIIVETVNEHFLKTNKRINIPGVNFSLPFLSQKDIDDIIWGAKYKVDYIAASFVNSAKNVKEIRDILNANGGENIQIISKIESKMAINNIDEIIEASDGIMVARGDLGLEIPYYDVPYYEKKIIRKCRLVGKEVIVATQMLDSMENNPIPTRAEVTDVYYAVELGADSTMLSGESASGKYPVEAVKVMSKIAKRAEKEYYNELFYGKQIEKVANHPTSRMLIAYEVAKILQSGEYKFAIILSHTGKLLKEVAKYRPNAFIVGVVSDNRLV", "aa_hexdigest": "bcbe4b59702b4bb9be268bf04bb2ce4b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8K6", "query_cov": 1.0, "subject_cov": 0.879492600422833, "identity": 0.998, "score": 808.0, "evalue": 1.47e-294, "valid": true, "gene": "pyk", "product": "pyruvate kinase", "ec_ids": [ "2.7.1.40" ], "uniref50_id": "UniRef50_L0RUB4", "cog_id": "COG0469", "cog_category": "G", "kegg_orthology_id": "K00873", "go_ids": [ "GO:0000287", "GO:0004743", "GO:0006096", "GO:0030955" ] }, "pscc": { "uniref50_id": "UniRef50_L0RUB4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_L0RUB4" ], "product": "Pyruvate kinase" }, "genes": [ "pyk" ], "id": "GLJLFKCOHC_937", "locus": "GCA013368035_00936" }, { "type": "cds", "sequence": "contig_1", "start": 540294, "stop": 541013, "strand": "-", "frame": 3, "gene": null, "product": "Restriction endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q098", "UniRef:UniRef50_UPI001E622C03", "UniRef:UniRef90_A0A6A8Q098", "UniRef:UniRef90_UPI001E622C03" ], "nt": "ATGTGTAAATTGGGTTTATATAATAGCAATTCAAAAACGATTTTAAAATCTGAATTGAATAATTTTTTTAATTTAGAAAATTTGAAATATTTAAATGAAGCCAATTTTTATTCATTGCCAATTTTTAAGGTGGATAATATTGAAAAAAATATCAAGTTTCCATTTAAATATGAAGAAGTAATAGATATTTTAAATCAAATTCAAAGTGAAAAATTTGAATTTAAAGAAGCACCATCAGAAAAATACATTGCCCTAAAACACGTGCCAAATTTTGCGTTAGCAGAATATTTTGTTAATTTATTCTTCACATATTCCCTTAATATTAATCCGCAAAATTTTAAAAGTATATGTCATACAAAATTAACAAAGAATTTGTATCCAATTTTTTCTGCTCCCGGACATTCGGCAGATTTCGATTATTATGATTCAAAAAACAACATTCAGTATATAGTTGAAACGACTATACTAAAAACAGAGAAGGAAGTTGTTGATGGTGAAAATTATTCAATATCAAAACATGCACAAAACCTAGTTAACGCAAATCAAAATATAAAGGAAATTAATTTATATTTTGTAAATTTTTTAAAATTTAACGAAGAACAAAAAAAAGAATTAGAATCCGTATTTTTATCTACATATAAATCAATGCTTGAAAAAAATAATGTTGGGAAAAATATTTATATAAAAAAATTCTCTGAATTAATTAATTTAAATTTATAA", "aa": "MCKLGLYNSNSKTILKSELNNFFNLENLKYLNEANFYSLPIFKVDNIEKNIKFPFKYEEVIDILNQIQSEKFEFKEAPSEKYIALKHVPNFALAEYFVNLFFTYSLNINPQNFKSICHTKLTKNLYPIFSAPGHSADFDYYDSKNNIQYIVETTILKTEKEVVDGENYSISKHAQNLVNANQNIKEINLYFVNFLKFNEEQKKELESVFLSTYKSMLEKNNVGKNIYIKKFSELINLNL", "aa_hexdigest": "ff4f157e3b334aa320deb5bf80c44e52", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI001E622C03", "query_cov": 0.8117154811715481, "subject_cov": 0.8899082568807339, "identity": 1.0, "score": 385.0, "evalue": 2.84e-134, "valid": true, "uniref50_id": "UniRef50_UPI001E622C03" }, "pscc": { "uniref50_id": "UniRef50_UPI001E622C03", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E622C03" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 540294, "stop": 541313, "observations": { "mutation": [ 541016 ], "stop": [ 541016 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8Q098", "query_cov": 1.0, "subject_cov": 0.45610687022900764, "identity": 1.0, "score": 468.0, "evalue": 2.25e-162, "gene_start": 1, "gene_end": 239, "reference_start": 286, "reference_end": 524, "reference_sequence": "MKKQGFNKFGGTEFRVAEPINEYFSILSWVNYERISIENNLSNNLQKNFNVNWDEEFKKYVKNQKIYNYSNDKKIKSRFNFVISHLKKWGFLKNSKNLVFNFEPKKFFDTDSPVEELFFKKMYLYWEEFREMVNFIISCNESSISIEKLNWAFAVFDYKEDKFEDLYAKNKQQILEEIADVSNITNIKSLFKKKINEYKILEKIFNLLRIKEPLSYSDYLELKPKEFKYIKRLLNIPPKERNVNYLDKINFYIFNHLQEDFLLDLKKISIEFNIEKEYMDMFNRWMCKLGLYNSNSKTILKSELNNFFNLENLKYLNEANFYSLPIFKVDNIEKNIKFPFKYEEVIDILNQIQSEKFEFKEAPSEKYIALKHVPNFALAEYFVNLFFTYSLNINPQNFKSICHTKLTKNLYPIFSAPGHSADFDYYDSKNNIQYIVETTILKTEKEVVDGENYSISKHAQNLVNANQNIKEINLYFVNFLKFNEEQKKELESVFLSTYKSMLEKNNVGKNIYIKKFSELINLNL" }, "paralog": false, "identity": 0.9970501474926253, "score": 677.0, "evalue": 3.77e-247, "description": "Internal stop codon at 541016. Nonsense mutation around 541016.", "psc": { "uniref90_id": "UniRef90_A0A6A8Q098", "product": "Restriction endonuclease", "uniref50_id": "UniRef50_A0A6A8Q098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8Q098", "UniRef:UniRef50_A0A6A8Q098" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8Q098", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8Q098" ], "product": "Restriction endonuclease" } }, "id": "GLJLFKCOHC_938", "locus": "GCA013368035_00937" }, { "type": "cds", "sequence": "contig_1", "start": 541014, "stop": 541250, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAAGAATCAAAGAACCTTTGTCATATAGTGATTATTTAGAATTGAAACCCAAAGAATTTAAATATATAAAAAGATTATTAAATATTCCCCCAAAAGAAAGAAATGTAAACTATTTAGATAAAATAAATTTTTACATATTTAATCATTTACAAGAAGATTTTTTGCTAGATTTAAAAAAAATCTCTATTGAATTTAATATCGAAAAAGAATATATGGACATGTTCAATAGATGA", "aa": "MLRIKEPLSYSDYLELKPKEFKYIKRLLNIPPKERNVNYLDKINFYIFNHLQEDFLLDLKKISIEFNIEKEYMDMFNR", "aa_hexdigest": "26b7e4e233c6063d320d028564fd0e27", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9751.304899999996, "isoelectric_point": 7.901583671569825 }, "id": "GLJLFKCOHC_939", "locus": "GCA013368035_00938" }, { "type": "cds", "sequence": "contig_1", "start": 542095, "stop": 542520, "strand": "-", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "SO:0001217", "UniRef:UniRef50_A0A6A8PYM9", "UniRef:UniRef90_A0A6A8PYM9" ], "nt": "ATGATATATATATATATATATATATATAAGAAGTATGGCTCAATTGATAAATTAGATCCCGTAACGAAAAGAGTAAATTCATCAATTTATAATTCGATAAAAAGTGAATATAATTTATCAAAAAATAAGTATTCATATGAATCTTGTATAGTTTTATTAGTTCTAATAATTTATGGATTTAATAGTCAAATAAGATTTAATTCAAAAGGAGAATTTAATATTCCTTCCGGAAAACAAATTTTAAATGAAACAAGGAAACAATGCCTTGTCGATTTTTCTTCAACTTTAAATAGAAAAAATATTGATTTTTCTTCATTGGATTTTCAAGAATTTATATATTTGAAAATGCAAGAAACAAGAGAAGACGATTTTTGGTATTTTGATCCTCCATACTTAATTTCGGGTGCAATATATAATCAGGCCTGA", "aa": "MIYIYIYIYKKYGSIDKLDPVTKRVNSSIYNSIKSEYNLSKNKYSYESCIVLLVLIIYGFNSQIRFNSKGEFNIPSGKQILNETRKQCLVDFSSTLNRKNIDFSSLDFQEFIYLKMQETREDDFWYFDPPYLISGAIYNQA", "aa_hexdigest": "9a9c905308e20e14d284c478182ff584", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 541849, "stop": 542572, "observations": { "insertion": [ 542512 ], "stop": [ 542031, 542097 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PYM9", "query_cov": 0.9787234042553191, "subject_cov": 0.5774058577405857, "identity": 1.0, "score": 279.0, "evalue": 1.23e-93, "gene_start": 4, "gene_end": 141, "reference_start": 20, "reference_end": 157, "reference_sequence": "MCNIVKLLSNTNPIKILNDIYIYIYKKYGSIDKLDPVTKRVNSSIYNSIKSEYNLSKNKYSYESCIVLLVLIIYGFNSQIRFNSKGEFNIPSGKQILNETRKQCLVDFSSTLNRKNIDFSSLDFQEFIYLKMQETREDDFWYFDPPYLISGAIYNQAWTLEEDRKLFKLLDLLDSKNIKWGLSNLFQSKGKSNTELIEWSKKYKVFHLNKQYTNSNYQRKNRDQLDEEVYVTNYEKAGI" }, "paralog": false, "identity": 0.9791666666666666, "score": 463.0, "evalue": 1.06e-168, "description": "Internal stop codon at 542031, 542097. Frameshift due to insertion around 542512.", "psc": { "uniref90_id": "UniRef90_A0A6A8PYM9", "product": "site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A6A8PYM9", "kegg_orthology_id": "K06223", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PYM9", "EC:2.1.1.72", "UniRef:UniRef50_A0A6A8PYM9", "KEGG:K06223" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PYM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PYM9" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" } }, "id": "GLJLFKCOHC_940", "locus": "GCA013368035_00939" }, { "type": "cds", "sequence": "contig_1", "start": 542492, "stop": 542872, "strand": "-", "frame": 1, "gene": null, "product": "DNA methyltransferase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BBF45A" ], "nt": "ATGGGTTCTGGTTCGACTTGTGTTGCGGCCTTAAAATTGCAAAGGAATTTTATTGGATGTGAGATAGATAAAAAATATTTTTGTGCTGCAAAGCAAAGAATAGAAAGGTATGAGCTAGAAATGAATAAAATGAATAGATCACCATTGTATTATTTGGGCGATAAATATAAATTATTACCACAATTAAAAAAATTATTTCCAAATAATATTAGAAATTTTTATGATGCATTTGCCGGAGGAGGAACGGTTATTGCAAATGTTATAGCTCAAAAATATTTTTACAATGATAAGCAAAAAGAAGTGTGCAATATTGTAAAACTTCTCTCAAATACTAACCCTATAAAAATTTTAAATGATATATATATATATATATATATATAA", "aa": "MGSGSTCVAALKLQRNFIGCEIDKKYFCAAKQRIERYELEMNKMNRSPLYYLGDKYKLLPQLKKLFPNNIRNFYDAFAGGGTVIANVIAQKYFYNDKQKEVCNIVKLLSNTNPIKILNDIYIYIYI", "aa_hexdigest": "056c446d02ba95eae2c1974a810e8ed7", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0035BBF45A", "query_cov": 0.9920634920634921, "subject_cov": 0.8620689655172413, "identity": 0.568, "score": 137.0, "evalue": 3.07e-39, "valid": false, "product": "DNA methyltransferase", "uniref50_id": "UniRef50_UPI0035BBF45A" }, "pscc": { "uniref50_id": "UniRef50_UPI0035BBF45A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0035BBF45A" ], "product": "DNA methyltransferase" }, "genes": [], "id": "GLJLFKCOHC_941", "locus": "GCA013368035_00940" }, { "type": "cds", "sequence": "contig_1", "start": 544318, "stop": 544788, "strand": "-", "frame": 2, "gene": null, "product": "Mbov_0401 family ICE element transposase-like protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158F2D97", "UniRef:UniRef90_UPI00158F2D97" ], "nt": "TTGTTTAAAGATATTAAAAATAAGAATATTGGCTTTGAATTACGTAAAAAAGTAATCTCATTATATTTAAACGGGCTGAGTTATAGAAAAATAGCTAAAAATTATGGAATTAGCCACACAGAAATTTTTAAAATAGTCAAAAATCATTTTCCTCATTCTTTAAAAGAAGCAAAAATAATTAATTTTAAGGAAATTATAACCAAAAATGAGAATATTTATATTGCAGTAGATGATACTTATTTAAAAGTACGACAAGGTCAAAATGAAATAAACAAAATTAAAGCAAGAATAATTAATTTCTTTGCATTAGATAAAAATAAAAAGCCTTATTGCAAAAACCATTTGATTTATTTTTCGAGCCCAAATAGAAATATGAATTTAAATGATTTAGTAGAAAAAATTAATTCTGTAATTAGGAATAATTATTCAGAAGAAGCCAATATTATAATCACAGGAGACGGGGCAAAATGA", "aa": "MFKDIKNKNIGFELRKKVISLYLNGLSYRKIAKNYGISHTEIFKIVKNHFPHSLKEAKIINFKEIITKNENIYIAVDDTYLKVRQGQNEINKIKARIINFFALDKNKKPYCKNHLIYFSSPNRNMNLNDLVEKINSVIRNNYSEEANIIITGDGAK", "aa_hexdigest": "5689647cb71ec80d10d6873ab4a03c3a", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 544015, "stop": 544995, "observations": { "mutation": [ 544104, 544320, 544896, 544911 ], "stop": [ 544104, 544320, 544896, 544911 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158F2D97", "query_cov": 1.0, "subject_cov": 0.40310077519379844, "identity": 0.981, "score": 306.0, "evalue": 6.6e-102, "gene_start": 1, "gene_end": 156, "reference_start": 70, "reference_end": 225, "reference_sequence": "MEAIEIIKEKIINEDIEFFNSVARKNQGWRVKDWIYRKLTFEFGTIRVKIRKYVRKIDNKYENYNVCLSLFKDIKNKNIGFELRKKVISLYLNGLSYRKIAKNYGISHTEIFKIVKNHFPHSLKEAKIINFKEFINKNENIYIAVDDTYLKVRQGQNEINKIKARIINFFALDKNKKPYCKNHLIYFSSPNRNMNLNDLVEKINSVIRNNYSEEANIIITGDGAKWIRTLSKKLSARYILCKFHLHSKFQVIFNHSNHLKKELEKLEKITKFNLFNILYTYLKNHKYIEIFNLVSKHWDSIKKCLSEKRAKLLHDFAYYVHLNITGLSEIEECDKNYYGNIAESYVSHIIKSKTKKFYSICNIKTIIQRIINPYDYIKNNIEIHIID" }, "paralog": false, "identity": 0.9785276073619632, "score": 606.0, "evalue": 9.74e-223, "description": "Internal stop codon at 544104, 544320, 544896, 544911. Nonsense mutation around 544104, 544320, 544896, 544911.", "psc": { "uniref90_id": "UniRef90_UPI00158F2D97", "product": "Mbov_0401 family ICE element transposase-like protein", "uniref50_id": "UniRef50_UPI00158F2D97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158F2D97", "UniRef:UniRef50_UPI00158F2D97" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00158F2D97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158F2D97" ], "product": "Mbov_0401 family ICE element transposase-like protein" } }, "id": "GLJLFKCOHC_942", "locus": "GCA013368035_00941" }, { "type": "cds", "sequence": "contig_1", "start": 545182, "stop": 545325, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGGCCTTAATATCTAAAAAAGGTAAAAATGAACTAACATTGACAATAAAACCTTCTAATGATAGTTGCGAAGGTGAAACAAAATTAAAAGTTATTAACGATTTTGATGGCGACTTATCTAAAACAGAAGATAAACACTGA", "aa": "MKALISKKGKNELTLTIKPSNDSCEGETKLKVINDFDGDLSKTEDKH", "aa_hexdigest": "e882d149d97cae615e0f0e176c9df117", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5220.882400000001, "isoelectric_point": 6.54133815765381 }, "id": "GLJLFKCOHC_943", "locus": "GCA013368035_00942" }, { "type": "cds", "sequence": "contig_1", "start": 545416, "stop": 545724, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTAGTTAAAATAGAAATTCAAAAGCAATTAAATTCCTTAAATCCCGAATTAGTTTTGACTAAAAATGTGATTTATAATGAACAAGAATTTGAAAACATAATTACTAATTTATTTTATAAAGACATTATTTCAACATTGAATATATTTCCTAACCATCCAATGATAAAAAATTCTACTTCAATTGAAATTACTAATGGCCATAAAACAACACAAAAATTTAATTTTGGTGATTTAGAATTAGATGATTTAAACATTAAATCAAAAAATTACACAGAAATTAAAAAATTAATAATTCAATGA", "aa": "MANLVKIEIQKQLNSLNPELVLTKNVIYNEQEFENIITNLFYKDIISTLNIFPNHPMIKNSTSIEITNGHKTTQKFNFGDLELDDLNIKSKNYTEIKKLIIQ", "aa_hexdigest": "0d02170adc57aeb5bbae05dbadf484a3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11881.548199999983, "isoelectric_point": 6.079748725891112 }, "id": "GLJLFKCOHC_944", "locus": "GCA013368035_00943" }, { "type": "cds", "sequence": "contig_1", "start": 545731, "stop": 546195, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAATAACGAATTCAAAATTGAGGCAATTTTTAAACCGGTTGATTTATTTGATGATAGATTTGAACCTAAAATGATTAATTTAAAAGGTGAAACAAGAGAAGAACAAATAAAACAAATAATTACTCTTGAAATAAAGAAACAAATTATTCAATTTTTTAAAAAATTTGATATTGAGGTATCTGAAAATGATATAAAAATTCAAGAAATTACAAGTGAAATTGTACAAAAATTGAAAAATGTTTATAAAACAAAAGAAATTGCGTTAAACAATGCAACAAAACTAAATGTTGATATAAATAATAATAAACATAATATAAAAGCAAAAACTACATTTGAAATTATTAATTTTTCAGATACTTATGTTTCAAATATTATTGATTTAAGCAAACTAAAACTTGACCCTATTGATATAAATTATGATGTTTCTTTTTCAAATGATAAAGAATATCAAGCAAAATGA", "aa": "MTNNEFKIEAIFKPVDLFDDRFEPKMINLKGETREEQIKQIITLEIKKQIIQFFKKFDIEVSENDIKIQEITSEIVQKLKNVYKTKEIALNNATKLNVDINNNKHNIKAKTTFEIINFSDTYVSNIIDLSKLKLDPIDINYDVSFSNDKEYQAK", "aa_hexdigest": "cabea64516598edcacf3b06478fe0bc9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18123.55299999999, "isoelectric_point": 5.579167747497558 }, "id": "GLJLFKCOHC_945", "locus": "GCA013368035_00944" }, { "type": "cds", "sequence": "contig_1", "start": 546709, "stop": 546918, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCTATAACCAATTTAGCATATGAAAAAGATTATTTAATTGAAGAACTTAACAACATAGATTACATGAAGGCTTTAATATCTAAAAAAGGTAAGAATGAACTAACATTAACCATAAAACCTGTTAATATGAGTTATGAAGGCGAAACGAAATTAAAGGTTATTAACGATTTCGATGGCAATTTATCGGAAGAAGATAAAAAAACCTGA", "aa": "MSITNLAYEKDYLIEELNNIDYMKALISKKGKNELTLTIKPVNMSYEGETKLKVINDFDGNLSEEDKKT", "aa_hexdigest": "cc8cb716e0ab989cecc350c76dfab058", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7946.982099999999, "isoelectric_point": 4.850491905212402 }, "id": "GLJLFKCOHC_946", "locus": "GCA013368035_00945" }, { "type": "cds", "sequence": "contig_1", "start": 546943, "stop": 547251, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAATTTAGTTAAAATAGAAATTCAAAAGCAATTAAATTCCTTAAATCCTGAATTAGTTTTGACTAAAAATGTGATTTATAATGAACAAGAATTTGAAAACATAATTACTAATTTATTTTATAAAGACATTATTTCAACATTAAATATATTTTCTAACCATCCAATGATAAAAAATTCTACTTCAATTGAAATTACTAATAGCCACAAAACAACACAAAAATTTAATTTTGGTGATTTAGAACTAGATGATTTAAACATTAAATCAAAAAATTACACAGAAATTAAAAAATTAATAATTGAATGA", "aa": "MANLVKIEIQKQLNSLNPELVLTKNVIYNEQEFENIITNLFYKDIISTLNIFSNHPMIKNSTSIEITNSHKTTQKFNFGDLELDDLNIKSKNYTEIKKLIIE", "aa_hexdigest": "d79375a23f42b11982c0dfbf4d139550", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11902.521099999984, "isoelectric_point": 5.6736341476440435 }, "id": "GLJLFKCOHC_947", "locus": "GCA013368035_00946" }, { "type": "cds", "sequence": "contig_1", "start": 547258, "stop": 547722, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACTAATAACGAATTCAAAATTGAGGCAATTTTTAAACCAGTTGATTTATTTGATGATAGATTTGAACCTAAAATGATTAATTTAAAAGGTGAAACAAGAGAAGAACAAATAAAACAAATAATTACTCTTGAAATAAAGAAACAAATTATTCAATTTTTTAAGAAATTTGACATTGAAGTATCGGAAAATGACATAAAAATTCAAGAAATTACAAGTGAAATTGTACAAAAATTGAAAAATGTTTATAAAACAAAAGAAATTGCGTTAAACAATGCAACAAAACTAAATGTTGATATAAACAATAAAACACATAATATAAAAGCAAAAACCACATTTGAAATTATTAATTTTTCAGATACTTATGTTTCGAATATTATTGATTTAAGCAAACTAAAACTTGACCCTATTGATATAAATTATGATGTTTCTTTTTCAAATGATAAAGAATATCAAGCAAAATGA", "aa": "MTNNEFKIEAIFKPVDLFDDRFEPKMINLKGETREEQIKQIITLEIKKQIIQFFKKFDIEVSENDIKIQEITSEIVQKLKNVYKTKEIALNNATKLNVDINNKTHNIKAKTTFEIINFSDTYVSNIIDLSKLKLDPIDINYDVSFSNDKEYQAK", "aa_hexdigest": "818bbac4603834207d2171eb7153babd", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18110.55429999999, "isoelectric_point": 5.579167747497558 }, "id": "GLJLFKCOHC_948", "locus": "GCA013368035_00947" }, { "type": "cds", "sequence": "contig_1", "start": 547921, "stop": 548199, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATTTTTCATCAGAATCTAATAAATTTAAGGATAAAACCGAATTTTTAGAATATTTAAAACAAAATAATATCGATATTATTGATAAATATGGAAAATTTGAAAATAAAGAATTAGTAGGAATTGAAAAATATAATTTCATAGACAACAATCAACTAGAAATTACTTATAATCTAAAAACTGCTGATGACAGATATAGATTAACAGTCTTAAAGCAAACTTATTTAATTAATTTTATTAATGATAAAACAAATAAAACCGAAATATTTCTTCAATGA", "aa": "MDFSSESNKFKDKTEFLEYLKQNNIDIIDKYGKFENKELVGIEKYNFIDNNQLEITYNLKTADDRYRLTVLKQTYLINFINDKTNKTEIFLQ", "aa_hexdigest": "1dbffc7fc6fbeb6b6f7dfbc981c93c7a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11119.401199999986, "isoelectric_point": 5.132242774963378 }, "id": "GLJLFKCOHC_949", "locus": "GCA013368035_00948" }, { "type": "cds", "sequence": "contig_1", "start": 548365, "stop": 548514, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTCAAATGAAGGAACATATCTCTTATATTCTAGAAGCTCAAAAGATTCAATTAATAACTTCAAGATTTTAAAAATTAGCAATGAACAAACAATAAATAAGAAAATGTTTTCAGAAATAAACAAAATTGATTCAATTAAGAATTTATGA", "aa": "MSNEGTYLLYSRSSKDSINNFKILKISNEQTINKKMFSEINKIDSIKNL", "aa_hexdigest": "5497e751c08adb8b562fa440b50b585f", "start_type": "TTG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5699.468399999999, "isoelectric_point": 9.456109809875489 }, "id": "GLJLFKCOHC_950", "locus": "GCA013368035_00949" }, { "type": "cds", "sequence": "contig_1", "start": 548560, "stop": 548724, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCATTCTTTGCATACACATTGTTTAATTCAAAGGGTGATAAAATACGAGATAAGGGCTTTATTGCAGAGGCTACCGTAATCTCAAAAGCTGCTCTAAAAATGCTTGCCGGAAAAACAGAAGAGTATTACATAATTAAAATTGATAATAAATCCAAAAATTGA", "aa": "MPFFAYTLFNSKGDKIRDKGFIAEATVISKAALKMLAGKTEEYYIIKIDNKSKN", "aa_hexdigest": "7e6415fbf9a9072d15eab73596ada35a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6120.0999999999985, "isoelectric_point": 9.522834587097169 }, "id": "GLJLFKCOHC_951", "locus": "GCA013368035_00950" }, { "type": "cds", "sequence": "contig_1", "start": 548995, "stop": 549570, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAATACATCAAATGATGAAAAATTTATATTGATTGAAAATCAGGGTTTCGATTTTAAAAAATCTAATAAATTAGAAAAATTAGGAATAATAAATAAATTAAGGTTATCAGAAACTCAAAAATATTTAGCATTCGATCAAATAAATAACAATAATCCTACCGGAATGGCAAATGATAAGCCCGAAAGAATAGCAAGCGATAAAAAGATAGGTCTTGAAGGACAAAATTATTACCTAGGAACTTATAAATTACATGCTCCTGCTGTCTTTTCTTTTACGGCGCCTAATGACAATTATGTTGTTAAGGTAAATGGCCATAAAGTTGATTTAATTAATAACAAATTCAATATTAAACTTGCAGATAAAAGAGATGATGCAAGCGATGACCAAAAAATATACAATCAAGATTCTGATTCCTCCAAACCACAAGACGAAAAGAATTCGCATAAAAAAAATGAATATACAATTACTGTTGAAGAATATAGAAATATCAACGATCCATCAACAAAGACTATAACATATACCAAAAAATATATTATTGATTCAAAAAGTGGAAGTTTAGATTTTAAATGA", "aa": "MVNTSNDEKFILIENQGFDFKKSNKLEKLGIINKLRLSETQKYLAFDQINNNNPTGMANDKPERIASDKKIGLEGQNYYLGTYKLHAPAVFSFTAPNDNYVVKVNGHKVDLINNKFNIKLADKRDDASDDQKIYNQDSDSSKPQDEKNSHKKNEYTITVEEYRNINDPSTKTITYTKKYIIDSKSGSLDFK", "aa_hexdigest": "a22d6ffb1c73a02b6b24ec2e58f156ad", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21916.167100000006, "isoelectric_point": 8.474514198303225 }, "id": "GLJLFKCOHC_952", "locus": "GCA013368035_00951" }, { "type": "cds", "sequence": "contig_1", "start": 549619, "stop": 549846, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGGCCTAAATCACGCCAAAAAGCATTTGAATACTTTAAAAAAGAACTTTCTAAATTTATAGAAAGAGGAAAAAATGTTCAATTAATTACAGATTATAATCATGACAAAATTGATGATTATAAATTATTAGAGCCTGAATATGAGCAATTTAAAACTAACAGGCAATATTTTAAAGAGTGGGCAAAAAAACTAGATTTATCATTTTCTTATGAAAATTTCACTTGA", "aa": "MRPKSRQKAFEYFKKELSKFIERGKNVQLITDYNHDKIDDYKLLEPEYEQFKTNRQYFKEWAKKLDLSFSYENFT", "aa_hexdigest": "39172e7ed87f644ce54408ba8e3092ab", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9384.510699999997, "isoelectric_point": 9.09785900115967 }, "id": "GLJLFKCOHC_953", "locus": "GCA013368035_00952" }, { "type": "cds", "sequence": "contig_1", "start": 550528, "stop": 550845, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGGGAACGCAAAATTTTATGGATTTTAATCAAAATCAATTAGTTCAAACTAAATCTGCGGGGATTCTAGAAAATATGCAATATTCAATTACTCTAAGATCTAATTATCAAGATATAGAAGCATTAAAAACTCTATTCGAAAATAGAATGCCTTTAACAGAACAAGAATTGCAATTTTTATCTTCTGCTCCGATTGGGGGCGGAATATTATCCATTGATAACACAACAAGATTTCAATTCGAATCTTATTACAATGAATTAGAAAGAAAATTATTATTTAACAATGGCGATTTAAAAGCAATTGCAAATAATTAA", "aa": "MLGTQNFMDFNQNQLVQTKSAGILENMQYSITLRSNYQDIEALKTLFENRMPLTEQELQFLSSAPIGGGILSIDNTTRFQFESYYNELERKLLFNNGDLKAIANN", "aa_hexdigest": "0bf6a26f2302f1709a5f7afb74295a6c", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12063.43299999998, "isoelectric_point": 4.644507408142091 }, "id": "GLJLFKCOHC_954", "locus": "GCA013368035_00953" }, { "type": "cds", "sequence": "contig_1", "start": 551326, "stop": 552165, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF12846.13", "PFAM:PF01935.23", "PFAM:PF01580.24" ], "nt": "TTGAAAGAAAAGATATTAAATAAAGCATCAGCAATGGTGGAAATAAACAACAATAGCAAATCAAGATTTAAAACAGTTAAATCAAAGGTTGAAATGGAAGCAATTGAAAATCTTATTTATTTGGCAAATACAAAAAATATGAATATTTTTAATAGTAATTTTATTTTTGTAAATCAGGGCAATAATTTGGATGAAATTAATGCATTAGAAACCCAAAATCAATTAAACGCTTCTGAAATATCAGCGAACATAAACCCTTTATTATTTAAACAAAAACAAGCATATGCAAGTGCTTGTTTGATAAATGGCGATAATATTGGGGATGATTTAGAAATGGTTTCAGAAAATATTTCAAGAGGTTGGGGATTTGTCAATTCTGAATTAAATGATAAAACACCATTATTATTTGGCTTTGATATGACAACTGATTCACCTTTATTTTTGGATATAAAAACCAAAAATTCGCAAAGAGTTTATTCAAATATGCTGATTACCGGGACACCAGGTGCCGGCAAATCAACACTTATGAAAAAAATGGTATTGCATTATTTAATTTATGGCACTGAAATCATTATTTTAGACCCTCAAAGGGAATATACAAAATTTGCAAAATTATTTGATGGCGAAGTTTTTGAAATGGGAACAGGAATTAATACGTTAATTAATCCTCTTCAACTAGATTTGCAATTTAATGAACAAGTAGAAATTAAAAATCAAAATAAAATTATAATATCTTCAAATATTAGAAAAGTTCAAAAATTCCTTAAAATAATGTGTAATTTTAATGATATAGAAGTTAGATTTATCGGAAAATCCTTGTCAGAGTTATATAATAAATGA", "aa": "MKEKILNKASAMVEINNNSKSRFKTVKSKVEMEAIENLIYLANTKNMNIFNSNFIFVNQGNNLDEINALETQNQLNASEISANINPLLFKQKQAYASACLINGDNIGDDLEMVSENISRGWGFVNSELNDKTPLLFGFDMTTDSPLFLDIKTKNSQRVYSNMLITGTPGAGKSTLMKKMVLHYLIYGTEIIILDPQREYTKFAKLFDGEVFEMGTGINTLINPLQLDLQFNEQVEIKNQNKIIISSNIRKVQKFLKIMCNFNDIEVRFIGKSLSELYNK", "aa_hexdigest": "fd760804a4956af3cb5f318908dae5b1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF12846.13", "name": "AAA_10", "length": 74, "aa_cov": 0.26523297491039427, "hmm_cov": 0.8918918918918919, "evalue": 9.49180837625042e-10, "score": 33.38563919067383, "start": 153, "stop": 226 }, { "id": "PF01935.23", "name": "DUF87", "length": 73, "aa_cov": 0.25448028673835127, "hmm_cov": 1.0, "evalue": 4.1477329860342236e-09, "score": 32.22016906738281, "start": 159, "stop": 229 }, { "id": "PF01580.24", "name": "FtsK_SpoIIIE", "length": 56, "aa_cov": 0.2007168458781362, "hmm_cov": 0.8928571428571429, "evalue": 2.0837558354535718e-08, "score": 29.280803680419922, "start": 130, "stop": 185 } ], "seq_stats": { "molecular_weight": 31711.13990000001, "isoelectric_point": 6.645239830017091 }, "id": "GLJLFKCOHC_955", "locus": "GCA013368035_00954" }, { "type": "cds", "sequence": "contig_1", "start": 552229, "stop": 552933, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGGAGTAAGAAAATATGATATTAAGTCAAAAAATAATACTAGTTTTTTAATACCATACAGCGAAATAATTAATGATGAAATTGTAATTAATAGAAAGAATAATTTTGGAATATTTAATCATTTCAGTGTTGTTAAGATAAAAGGATTTAATGTTTTTGTAAATTCGGAGCAAGATAGAGAAGCGGAGTTTTTGAGATTTACAAATCTCTTAAATTCTGTTGATGATAAAATAACTATTATTAAAACCTCTGCGCCAATGGATTTAACTTCTAATATTAATGAAATTGATAAAAATTTAAATACCTCTTTTATTCAAAATAATAATCTAGCAAAAGAATATTGTTTATTGAATAAACAAGAATTAGAAACATTAAACGAAAAAGATAGCATTGATAATTATTATTTAGTTATTTATGGAAATTCTATTGAAGATTTAATGGCTAAAAGAACGAGAATAATTTTGCAATTAACAAATACAAAATTTAATCCTCAATTAGTAAAAGGAATTGATTTACTAAATGTATTAAATTTAATATTTAGAGTGCAATTAAATGACAAAAATTTATCTAATATTGAAAAGCCAGCACCATTACTTGCTCCTAAAAATGTAGAATTTTTTAAAAAGTATATGGTAGCTGATAATAAATATATTAGTTTTCAATCAATAAATGATTTTAATAACATTGAATTACAAGATGCTTGA", "aa": "MGVRKYDIKSKNNTSFLIPYSEIINDEIVINRKNNFGIFNHFSVVKIKGFNVFVNSEQDREAEFLRFTNLLNSVDDKITIIKTSAPMDLTSNINEIDKNLNTSFIQNNNLAKEYCLLNKQELETLNEKDSIDNYYLVIYGNSIEDLMAKRTRIILQLTNTKFNPQLVKGIDLLNVLNLIFRVQLNDKNLSNIEKPAPLLAPKNVEFFKKYMVADNKYISFQSINDFNNIELQDA", "aa_hexdigest": "66fa8224336f9429a845f1ef115bd431", "start_type": "GTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 27132.696400000026, "isoelectric_point": 5.974369239807129 }, "id": "GLJLFKCOHC_956", "locus": "GCA013368035_00955" }, { "type": "cds", "sequence": "contig_1", "start": 553028, "stop": 553180, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATATTGCTATTTTAAATTTTCAATTGAATTTATTAGTAAAACCGCTTAAAACAATTGCTAATGCTATTATTGCAAAAAATATTACAAAATCAAAAACTGTATATTTTCTTCATATTGTTAATTTTTTATCTGCTAATTTTTTATTTTGA", "aa": "MNIAILNFQLNLLVKPLKTIANAIIAKNITKSKTVYFLHIVNFLSANFLF", "aa_hexdigest": "4524df8624c32c332ed113865063a87e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5677.829699999998, "isoelectric_point": 10.301225852966304 }, "id": "GLJLFKCOHC_957", "locus": "GCA013368035_00956" }, { "type": "cds", "sequence": "contig_1", "start": 553172, "stop": 553927, "strand": "-", "frame": 1, "gene": null, "product": "Mbov_0396 family ICE element transmembrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TU60", "UniRef:UniRef90_UPI001939BB38" ], "nt": "TTGCTTCCAATTAAAGAAAAATTGACTAACTTAGGAATCTTAATAATACCTGACCTAGTTATCTATATTGGGGCAGCATATGCTACAAAAGCCCTTAATAAGTTAATTGGCGATTTGCTAGATATTCAAGTACCTGACTTAGGATTCAAACAAGCAGGTAGATTTATAAGAACAGGCGCAGGAATTATTGCTGGCGCTACAACTGCCGGAGCAGCCGTGGCAGCAGGCAATGCAACATTAGGATCAACTATTTCAAAGGGCGGTTTTTCAAACGCAATTGGAGGCTCTATCGGTAAGGCGGTTAATATATCGAGAATTAGACGAAATAAAGCATATGCAAAAGACTTGGGAATGAACAAAGAATTTATTAAATCTTCGTTTGAAAATGTAGGCAAAAATAAAGTAATAGAACAAAGAAAAATGCTTAATAATTTTAAAAGTATGGCCACTGATGAAAAAGGAAACCTACACAGAGAATCATTATATGATTTGGACTCTTTGAAGCAATATAAGGCATCAGAATTACAAAAATTCAAAGACAAACAAGAATTATATGATGAATCATATAAGTTTTACAAGAAAACAAATACAGACAGTTATTTAGAACAAAAACGAGCCGAATTGGAACATAACAAAACTCAAAATAAATATAAATACTCTATTTCAAAAGCGGAAAATCAATTAAAGCAATTAAATGATTTTGATTTAGAAAAAAACGATGTAAGAAAGGAAAATGAAAAAGATGCTTCAAAATAA", "aa": "MLPIKEKLTNLGILIIPDLVIYIGAAYATKALNKLIGDLLDIQVPDLGFKQAGRFIRTGAGIIAGATTAGAAVAAGNATLGSTISKGGFSNAIGGSIGKAVNISRIRRNKAYAKDLGMNKEFIKSSFENVGKNKVIEQRKMLNNFKSMATDEKGNLHRESLYDLDSLKQYKASELQKFKDKQELYDESYKFYKKTNTDSYLEQKRAELEHNKTQNKYKYSISKAENQLKQLNDFDLEKNDVRKENEKDASK", "aa_hexdigest": "381ff172c0125c5c8be1d802ebb02fd6", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 553172, "stop": 554227, "observations": { "mutation": [ 554011, 554146, 554206 ], "stop": [ 554011, 554146, 554206 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939BB38", "query_cov": 1.0, "subject_cov": 0.46828358208955223, "identity": 0.988, "score": 429.0, "evalue": 1.58e-146, "gene_start": 1, "gene_end": 251, "reference_start": 286, "reference_end": 536, "reference_sequence": "MLISAITILLSPILYAIFALFWGIFISFPFLILQGLFKVARFFGFELINYIIFKVNPGEYTFSFSDLPAAFLAFLLYGLIFTIIFIIISFAKYQIGKKNKNQNVEHLKFKNVLKQSFSAFLWALLLPFLIFVSNIVLAIIFYYFGQALELQTNMKGDIISLLYVALNDNAEWAKESVAMNNFAPMPFSDYVAQLVSTSWGLGLMFIKNTIISWVCVIMLGSLLVNIVKNCLNQFTLFMISPLVAAKSVEDNGRAIMNWKHKFVESVISIFISLFALNFFAIFILILLPIKEKLSNLGILIIPDLVIYIGAAYATKALNKLIGDLLDIQVPDLGFKQAGRFIRTGAGIIAGATTAGAAVAAGNATLGSTISKGGFSNAIGGSIGKAVNISRIRRNKAYAKDLGMNKEFIKSSFENVGKNKVIEQRKMLNNFKNMATDEKGNLHRESLYDLDSLKQYKASELQKFKDKQELYDESYKFYKKTNTDSYLEQKRAELEHNKTQNKYKYSISKAENQLKQLNDFDLEKNDVRKENEKDASK" }, "paralog": false, "identity": 0.9715099715099715, "score": 629.0, "evalue": 1.31e-227, "description": "Internal stop codon at 554011, 554146, 554206. Nonsense mutation around 554011, 554146, 554206.", "psc": { "uniref90_id": "UniRef90_UPI001939BB38", "product": "Mbov_0396 family ICE element transmembrane protein", "uniref50_id": "UniRef50_A0A3T0TU60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939BB38", "UniRef:UniRef50_A0A3T0TU60" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3T0TU60", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3T0TU60" ], "product": "Transmembrane protein" } }, "id": "GLJLFKCOHC_958", "locus": "GCA013368035_00957" }, { "type": "cds", "sequence": "contig_1", "start": 554417, "stop": 554782, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCTAATTTCAGCAATAACCATATTATTATCCCCAATTTTATATGCAATTTTTGCATTATTTTGGGGAATTTTTATTAGTTTTCCATTCCTTATATTGCAAGGTTTGTTCAAGGTAGCAAGATTTTTTGGTTTTGAATTAATTAACTATATTATTTTCAAAGTTAATCCAGGAGAATATACATTTAGTTTTTCAGATTTACCAGCGGCGTTTTTAGCGTTTTTACTATATGGATTAATTTTTACTATTATTTTTATAATTATTTCATTTGCTAAATATCAAATTGGCAAGAAAAACAAGAATCAAAACGTAGAACATTTAAAATTCAAGAATGTTTTAAAACAATCATTTTCGGCTTTTCTATGA", "aa": "MLISAITILLSPILYAIFALFWGIFISFPFLILQGLFKVARFFGFELINYIIFKVNPGEYTFSFSDLPAAFLAFLLYGLIFTIIFIIISFAKYQIGKKNKNQNVEHLKFKNVLKQSFSAFL", "aa_hexdigest": "95561a53507477f24da8ba870195de57", "start_type": "GTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13968.640499999978, "isoelectric_point": 9.744219017028808 }, "id": "GLJLFKCOHC_959", "locus": "GCA013368035_00958" }, { "type": "cds", "sequence": "contig_1", "start": 554792, "stop": 554995, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158C5032", "UniRef:UniRef90_UPI00158C5032" ], "nt": "ATGTTTATTTACCTTGTTGTTCTTTTTGCTCAGAAAACAAAAATAAAAATTCAATATGACAATAAATTAGAATATAAATCGGATGCAAAATACAAAAAAATAGATAAATTAACAAAGATATTTATATCAGTTTTTGCAATCTTGATAATTGCTGCATTAGTATTAATAACTGTTGGTATTGGTAATACTTATTTTATTAAATAG", "aa": "MFIYLVVLFAQKTKIKIQYDNKLEYKSDAKYKKIDKLTKIFISVFAILIIAALVLITVGIGNTYFIK", "aa_hexdigest": "8d52ab0a0ac9807bd3e4fcfc8f2319f2", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 554792, "stop": 555049, "observations": { "mutation": [ 555040 ], "stop": [ 555040 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00158C5032", "query_cov": 1.0, "subject_cov": 0.788235294117647, "identity": 1.0, "score": 119.0, "evalue": 4.82e-34, "gene_start": 1, "gene_end": 67, "reference_start": 19, "reference_end": 85, "reference_sequence": "MFHWTFLVAIALSPVVLAMFIYLVVLFAQKTKIKIQYDNKLEYKSDAKYKKIDKLTKIFISVFAILIIAALVLITVGIGNTYFIK" }, "paralog": false, "identity": 0.9882352941176471, "score": 159.0, "evalue": 1.95e-52, "description": "Internal stop codon at 555040. Nonsense mutation around 555040.", "psc": { "uniref90_id": "UniRef90_UPI00158C5032", "uniref50_id": "UniRef50_UPI00158C5032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00158C5032", "UniRef:UniRef50_UPI00158C5032" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00158C5032", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00158C5032" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7763.393099999996, "isoelectric_point": 9.77832279205322 }, "id": "GLJLFKCOHC_960", "locus": "GCA013368035_00959" }, { "type": "cds", "sequence": "contig_1", "start": 555145, "stop": 555360, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VBN4", "UniRef:UniRef90_UPI001588FCBF" ], "nt": "ATGAATAGTGGCACCTCTGGTGTAGAAACTCTTAAACAAGCATTAGGAACGTTGTTTGAGCAAATAAATCCATTTGTTTCACTGATAGCAGGTTATGCTGTTGTTATCTTTGCCTTAATTGCTATTGCTATGACATTCTTTGCGATTGCTTCATTGAAAACAGCCAAAACTCAAACTTCTAAGAAAGAAGCTAAATCTAGAATAGTGGGGATATGA", "aa": "MNSGTSGVETLKQALGTLFEQINPFVSLIAGYAVVIFALIAIAMTFFAIASLKTAKTQTSKKEAKSRIVGI", "aa_hexdigest": "f85a431a6ee3a2918905895972f9f87a", "start_type": "ATG", "rbs_motif": "AGGAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 555064, "stop": 555360, "observations": { "mutation": [ 555147 ], "stop": [ 555147 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001588FCBF", "query_cov": 1.0, "subject_cov": 0.7244897959183674, "identity": 1.0, "score": 128.0, "evalue": 3.69e-37, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNSGTSGVETLKQALGTLFEQINPFVSLIAGYAVVIFALIAIAMTFFAIASLKTAKTQTSKKEAKSRIVGIWLSFGFLIFFVVLIPVILKVATTFLAN" }, "paralog": false, "identity": 0.9897959183673469, "score": 180.0, "evalue": 3.23e-60, "description": "Internal stop codon at 555147. Nonsense mutation around 555147.", "psc": { "uniref90_id": "UniRef90_UPI001588FCBF", "uniref50_id": "UniRef50_A0A3Q9VBN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001588FCBF", "UniRef:UniRef50_A0A3Q9VBN4" ] }, "pscc": { "uniref50_id": "UniRef50_A0A3Q9VBN4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3Q9VBN4" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 7507.806999999999, "isoelectric_point": 9.873542594909669 }, "id": "GLJLFKCOHC_961", "locus": "GCA013368035_00960" }, { "type": "cds", "sequence": "contig_1", "start": 555686, "stop": 556075, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGGACTTTCTCAATATATTGAATCATTTATGGAAATAAGAACAATTAGCGATTCTTTATTTAAAATAATTGACTTATTTACGTTTATGATAAACAATATAAATGACCCTGACAAAACCTTCTTGGAGCAACTACAAGCAATGGGTCCAGAGAAATTAGATAAATTAAAACATCTGATAAAAAATAATTTAACAATTGAACAATTAATAGGCAATATAAAGATAGCCTTAGAATTAGACAATAAACTTTATGTATTTCCTGCTCCTATTGTCAAGAATTTTTATGACAAGGCAGGTCATTACAATAAAATCATTGATGCTTTAAATGAATTTTATAATCCCTTAGCATTTTTACTAGGTTTTATTACTGAAACAAATGACTTTTTTTGA", "aa": "MGLSQYIESFMEIRTISDSLFKIIDLFTFMINNINDPDKTFLEQLQAMGPEKLDKLKHLIKNNLTIEQLIGNIKIALELDNKLYVFPAPIVKNFYDKAGHYNKIIDALNEFYNPLAFLLGFITETNDFF", "aa_hexdigest": "a49527dc5ca2061aebcffe6b4d22ae77", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15000.25239999998, "isoelectric_point": 4.914549446105956 }, "id": "GLJLFKCOHC_962", "locus": "GCA013368035_00961" }, { "type": "cds", "sequence": "contig_1", "start": 556145, "stop": 556285, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTAAAAAGTCAAAAGAAGAGTATGTTAGAAAAATTAAGAATAAAAAAGTATTAGAAGATGACCCGAATTTTTTAATAAACTACGAAGGAAATTATCAATATTTTACCGAATCTAGAATTAAGGATAAAGATCCTTGA", "aa": "MFKKSKEEYVRKIKNKKVLEDDPNFLINYEGNYQYFTESRIKDKDP", "aa_hexdigest": "4d2e620f52d36f5a933b214b045bbc1e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5690.397000000002, "isoelectric_point": 8.925599479675295 }, "id": "GLJLFKCOHC_963", "locus": "GCA013368035_00962" }, { "type": "cds", "sequence": "contig_1", "start": 556295, "stop": 556459, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGGTTAATATTCATAAATTAAAAGTCCTTGATTCTAATGGAAAAGAAACAAATGAATATGAATCAAAAGAACAAAAATTACAAAGATATGCCCAAGCAAAAACTGTGATTTTAAATTATTCTAATGCAGTAGAAGAAGCACGCAATAAATTGCTAGGTAATTGA", "aa": "MVNIHKLKVLDSNGKETNEYESKEQKLQRYAQAKTVILNYSNAVEEARNKLLGN", "aa_hexdigest": "58db96d79887c49f6869f174161c3644", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6221.963600000001, "isoelectric_point": 9.049443244934082 }, "id": "GLJLFKCOHC_964", "locus": "GCA013368035_00963" }, { "type": "cds", "sequence": "contig_1", "start": 556487, "stop": 556645, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGATAAGCCCAAATCTACCAAACCAAATAATTCATATCCAAATGATTTGCCAAAGCCAAAACTGCCAAAGGTTCAAGAGGTAAAACCAGAAGATAGATTCGATACAAAACTTTTATATTCTTGTCCTGTTCCAGCAGTTTCTGACAAAAATTGA", "aa": "MYDKPKSTKPNNSYPNDLPKPKLPKVQEVKPEDRFDTKLLYSCPVPAVSDKN", "aa_hexdigest": "6627b61acc1d2f02263c1ece387f02f1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5961.774700000002, "isoelectric_point": 9.13157596588135 }, "id": "GLJLFKCOHC_965", "locus": "GCA013368035_00964" }, { "type": "cds", "sequence": "contig_1", "start": 557456, "stop": 557707, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACCTTAAACTTTTATTACCAAGTGCAATAAATGCTGAAAAGCAAGAATTAGAATATTTATCTGAACTTCTATTCAATTTTGCTATGGAAATTAATGCTGCTAACGATAAAAATAATGAAAAATTAAATAATTTATGCAGTGTAATTTCACAAAAAGCAGGAGATATAAACGAATATCTAAACGAGCCCGGAACTGATTATTTCTTTTATTTAGAATTGAAAACTATTAAAGAATATACAAAAAATTGA", "aa": "MDLKLLLPSAINAEKQELEYLSELLFNFAMEINAANDKNNEKLNNLCSVISQKAGDINEYLNEPGTDYFFYLELKTIKEYTKN", "aa_hexdigest": "5bf32fa01898b45f220a3b8fffe4280c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9598.755499999994, "isoelectric_point": 4.481663703918457 }, "id": "GLJLFKCOHC_966", "locus": "GCA013368035_00965" }, { "type": "cds", "sequence": "contig_1", "start": 557792, "stop": 558223, "strand": "-", "frame": 1, "gene": null, "product": "variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F073", "UniRef:UniRef90_UPI0034A4F073" ], "nt": "ATGAAACATTTCAAAAAACTATTAATTACATTAATCGCTGCTGTCTCAACGACTGCGTTGCCATTAGTGGCAGCATCTTGCAACAATGAAAAATTAGCATTAGAAAATGGCAAAAAAGAATATGCAAATAACTTGCAATTATTAAAAAATGCTAAATCCCAATTGCAAAATTTGAATGTTGAATCAGAGCATCTAAAAAAAATTGATGAAAATATTGAAAAATCGATTAAATTGACTTCTAAAAATGCCACTTATAAAGAATATGCAAAAGCAAACACAATATTATCTGAAACCTTAAAACTGACAACTCAATTAATTAACGATCATCCTAAACAAATTTATGAATACGCAAAGGGGATTCCTGAAATTCTTGAAAAATACGAATTGCATTTTTTAGAATTACCATTTTATTCAATGAAGCCAGAGCCATGA", "aa": "MKHFKKLLITLIAAVSTTALPLVAASCNNEKLALENGKKEYANNLQLLKNAKSQLQNLNVESEHLKKIDENIEKSIKLTSKNATYKEYAKANTILSETLKLTTQLINDHPKQIYEYAKGIPEILEKYELHFLELPFYSMKPEP", "aa_hexdigest": "8c6a7fdcc2f7f5ea873377c4666452eb", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 557489, "stop": 558223, "observations": { "mutation": [ 557794 ], "stop": [ 557794 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A4F073", "query_cov": 1.0, "subject_cov": 0.5257352941176471, "identity": 0.965, "score": 261.0, "evalue": 2.79e-86, "gene_start": 1, "gene_end": 143, "reference_start": 1, "reference_end": 143, "reference_sequence": "MKHFKKLLITLTAAVSMTALPLVAASCNNEKLALENGKKEYANNLQLLKNAKSQLQNLNIESEYLKKIDENIEKSIKLTSKNATYKEYAKANTILSETLKLATQLINDHPKQIYEYAKGIPEILEKYELHFLELPFYSMKPEPWIQPQNPEFDIKKAYEYSTGTPEIHGKEKMDLKLLLPSAINAEKQELEYLSELLFNFAMEINNANDKNNEKLNNLCSVISQKAGDINEYLNEPGTDYFFYLELKTIKEYTKNWFSQVKNEWKEFKKQNN" }, "paralog": false, "identity": 0.9672131147540983, "score": 468.0, "evalue": 4e-170, "description": "Internal stop codon at 557794. Nonsense mutation around 557794.", "psc": { "uniref90_id": "UniRef90_UPI0034A4F073", "product": "variable surface lipoprotein", "uniref50_id": "UniRef50_UPI0034A4F073", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A4F073", "UniRef:UniRef50_UPI0034A4F073" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A4F073", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A4F073" ], "product": "variable surface lipoprotein" } }, "id": "GLJLFKCOHC_967", "locus": "GCA013368035_00966" }, { "type": "cds", "sequence": "contig_1", "start": 558634, "stop": 558921, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939E4D6", "UniRef:UniRef90_UPI001939E4D6" ], "nt": "ATGTCTAGAAAAAATTTTGAAGATCAGTATTTTAATTTCGTTTCAAGAGAAAATGGCGAAAATAGAGTAAATGGATATTTTGTTGATATATTAGATTCTATAAAAGACACTGATTATGAGCAAGATGCATACATTCAATATAGAAGATTTATAGATAAAATTGAAAATACACCAATTACTTACACCATTTATGATGCTTTTATTGATGATTTGCAACAGAATAAACTATTAGAAAACAAATTTATCGATTATTATAGAAAGGAAATAGATACCGACATATATGATTGA", "aa": "MSRKNFEDQYFNFVSRENGENRVNGYFVDILDSIKDTDYEQDAYIQYRRFIDKIENTPITYTIYDAFIDDLQQNKLLENKFIDYYRKEIDTDIYD", "aa_hexdigest": "41803d3666b35a57196ffe3f77aac208", "start_type": "ATG", "rbs_motif": "AGxAG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 558331, "stop": 558921, "observations": { "mutation": [ 558522, 558636 ], "stop": [ 558522, 558636 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939E4D6", "query_cov": 1.0, "subject_cov": 0.4377880184331797, "identity": 0.9470000000000001, "score": 184.0, "evalue": 3.32e-57, "gene_start": 1, "gene_end": 95, "reference_start": 1, "reference_end": 95, "reference_sequence": "MSRKNFEDQYFNFVSRENGENRVNGYFVDILNSIKDTDYEQDAYIQYRRFIDKIENTPITCTIYDAFIEDLQQNKLLENKFIDYYKKKIDTDIYDWYDAKTKEIDFDNLLKTLINRYSYSIPQKEFELLFEKSWNNQEGYHEGFLIEPNIGFYDAFRNDLEYILDDLDYSQEDKKHFLNKYIETINNECERIINSIRREQKPNLLFIHPYDNDRGIY" }, "paralog": false, "identity": 0.9591836734693877, "score": 387.0, "evalue": 8.08e-140, "description": "Internal stop codon at 558522, 558636. Nonsense mutation around 558522, 558636.", "psc": { "uniref90_id": "UniRef90_UPI001939E4D6", "uniref50_id": "UniRef50_UPI001939E4D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939E4D6", "UniRef:UniRef50_UPI001939E4D6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001939E4D6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939E4D6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 11700.678299999989, "isoelectric_point": 4.36593952178955 }, "id": "GLJLFKCOHC_968", "locus": "GCA013368035_00967" }, { "type": "cds", "sequence": "contig_1", "start": 559011, "stop": 559256, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATGCATCAATAATAAAAGGCAATATTGATTTGTTTGGTTTTACTATTTTCTCAGAGATAAATTTAGGGCTTCAATTAGCAAGTAATTCATTTAAGACCTTAAAATATTCAAAAAAGTCTTTGCAAAATTCCTTTGGCCAATCTTTTTCAAAATTAAATCATGATGATTTTCCGGCCAAAACATCACAAGCATCATATACACAATATTCAAAATCTGACAGTATTTTTTCTCGGTATATTTGA", "aa": "MYASIIKGNIDLFGFTIFSEINLGLQLASNSFKTLKYSKKSLQNSFGQSFSKLNHDDFPAKTSQASYTQYSKSDSIFSRYI", "aa_hexdigest": "fed66ea0630e3d766b63fae2d72da600", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9166.207199999997, "isoelectric_point": 9.477255439758302 }, "id": "GLJLFKCOHC_969", "locus": "GCA013368035_00968" }, { "type": "cds", "sequence": "contig_1", "start": 559172, "stop": 559387, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A0D95F", "UniRef:UniRef90_UPI0034A0D95F" ], "nt": "ATGAATAAAAATATGTCAATAGTTAGCAAAAGTTTAAGAAATTTTTTTAATTCTTTGCCTGAAAAAAAGTTTAATTCAAAAAAAATCACCGAATATAAAGAAAATCATTTACAAGCGATTGAAGAATTTGTTCAAATATACCGAGAAAAAATACTGTCAGATTTTGAATATTGTGTATATGATGCTTGTGATGTTTTGGCCGGAAAATCATCATGA", "aa": "MNKNMSIVSKSLRNFFNSLPEKKFNSKKITEYKENHLQAIEEFVQIYREKILSDFEYCVYDACDVLAGKSS", "aa_hexdigest": "1f8a74a71db86b0daeb013d54a25e233", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 558914, "stop": 559387, "observations": { "mutation": [ 559087, 559174 ], "stop": [ 559087, 559174 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A0D95F", "query_cov": 1.0, "subject_cov": 0.45222929936305734, "identity": 0.958, "score": 134.0, "evalue": 7.9e-39, "gene_start": 1, "gene_end": 71, "reference_start": 1, "reference_end": 71, "reference_sequence": "MNKNTLIVSKSLRNFFNSLPEKKFNSKKITEYKENHLQAIEEFVQIYQEKILSDFEYCVYDACDVLAGKSSWFNFEKDWPKEFCKDFFEYFKVLNELLANWSPKFISEKIVKPNKSILPFIIDAYNIRLLFSNEALIKEYAKYYLKQNKLVESGENV" }, "paralog": false, "identity": 0.9681528662420382, "score": 305.0, "evalue": 7.26e-109, "description": "Internal stop codon at 559087, 559174. Nonsense mutation around 559087, 559174.", "psc": { "uniref90_id": "UniRef90_UPI0034A0D95F", "uniref50_id": "UniRef50_UPI0034A0D95F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A0D95F", "UniRef:UniRef50_UPI0034A0D95F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A0D95F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A0D95F" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8361.472999999996, "isoelectric_point": 7.7399587631225595 }, "id": "GLJLFKCOHC_970", "locus": "GCA013368035_00969" }, { "type": "cds", "sequence": "contig_1", "start": 559380, "stop": 559604, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A281B", "UniRef:UniRef90_UPI00193A281B" ], "nt": "GTGTTAGATTTTGATTCTGCATACGAATGGGCAAATCAAGAACTTAAAGAAAAAGGAATGGAAGCGGAGGGGTGGGCATTCGATATTGAAAAAATTTCAAATATAGAAACCTTTAATGTTGAATTTATTGCATTTAATGCTTATAATAGAGCACTCATAATTTCCAGAGAAGATGCACAAAAATATACAGATAACTCTGAAAGAGATAAAGATTATAATGAATAA", "aa": "MLDFDSAYEWANQELKEKGMEAEGWAFDIEKISNIETFNVEFIAFNAYNRALIISREDAQKYTDNSERDKDYNE", "aa_hexdigest": "567c60002717a23bbf030971a90d7c49", "start_type": "GTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 559380, "stop": 559673, "observations": { "mutation": [ 559628 ], "stop": [ 559628 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193A281B", "query_cov": 1.0, "subject_cov": 0.7628865979381443, "identity": 0.973, "score": 144.0, "evalue": 1.59e-43, "gene_start": 1, "gene_end": 74, "reference_start": 24, "reference_end": 97, "reference_sequence": "MAIMTLSEIKRDLDYWKTHSPECVLDFDSAYEWANQELKEKGMEAEGWAFDIEKISNIETFNVEFIAFNAYNRALIISREDAQKYIDNSERDKDYNE" }, "paralog": false, "identity": 0.9690721649484536, "score": 193.0, "evalue": 1.74e-65, "description": "Internal stop codon at 559628. Nonsense mutation around 559628.", "psc": { "uniref90_id": "UniRef90_UPI00193A281B", "uniref50_id": "UniRef50_UPI00193A281B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193A281B", "UniRef:UniRef50_UPI00193A281B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193A281B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193A281B" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8782.442099999993, "isoelectric_point": 4.2705068588256845 }, "id": "GLJLFKCOHC_971", "locus": "GCA013368035_00970" }, { "type": "cds", "sequence": "contig_1", "start": 559682, "stop": 559837, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6", "UniRef:UniRef90_UPI0034A3CAD8" ], "nt": "ATGCAAAACGATTTTTATAATATTGAATTTGTGGGCATTGACGCCTATGGTAGAGCTTTCATCATGAGCAGAGAAGATGCCGAAAGATTTTTTCAAAATCAAGAAATAGAGGAAAACAAATCCTCTAAATCCCAATCAAAATCAAAAACTTTATAA", "aa": "MQNDFYNIEFVGIDAYGRAFIMSREDAERFFQNQEIEENKSSKSQSKSKTL", "aa_hexdigest": "837ca6fd14dec4117e6d022b46bd8a44", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 559682, "stop": 560008, "observations": { "mutation": [ 559867, 559918, 559963 ], "stop": [ 559867, 559918, 559963 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A3CAD8", "query_cov": 1.0, "subject_cov": 0.4722222222222222, "identity": 0.98, "score": 95.9, "evalue": 1.33e-24, "gene_start": 1, "gene_end": 51, "reference_start": 58, "reference_end": 108, "reference_sequence": "MAIMTLSEIKKDLDYWKEHEPQDVYTLNDAWELADQELMKYGMEADLWAFDINNIYEMQNDFYNIEFVGIDAYGRAFIMSREDAERFFQNQEIEENKSSESQSKSKTL" }, "paralog": false, "identity": 0.9351851851851852, "score": 199.0, "evalue": 4.26e-68, "description": "Internal stop codon at 559867, 559918, 559963. Nonsense mutation around 559867, 559918, 559963.", "psc": { "uniref90_id": "UniRef90_UPI0034A3CAD8", "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A3CAD8", "UniRef:UniRef50_A0A6A8PZW6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZW6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZW6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6028.565399999999, "isoelectric_point": 4.893632698059082 }, "id": "GLJLFKCOHC_972", "locus": "GCA013368035_00971" }, { "type": "cds", "sequence": "contig_1", "start": 561010, "stop": 561177, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATCTTGTGCTTATAAATTTATTAATTCTTTATTAAAAATATTAGGCCAAGAAAAATATGAAACTTTGTTTAAAGTTGAACATAAATTCGACAAAGTTTCACAAAAATTTACAACAGAAAGAACGTTATTGATAAGACTTAATAATAATTATGCATTTTAG", "aa": "MKKSCAYKFINSLLKILGQEKYETLFKVEHKFDKVSQKFTTERTLLIRLNNNYAF", "aa_hexdigest": "843d2070ebc535958c09954ecc8f5f65", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6619.709000000001, "isoelectric_point": 9.723266792297363 }, "id": "GLJLFKCOHC_973", "locus": "GCA013368035_00972" }, { "type": "cds", "sequence": "contig_1", "start": 562042, "stop": 562170, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATGGTTCAGGCTTAGCTATTGATAGAGTTGTTGCTGCTATTTTAGAAAATTATCAAAACCCAGATGGTTCAATCACAATTCCAAAAGTTTTAGTTCCATACATGGGCAAAGAAGTTATTAAATAA", "aa": "MNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK", "aa_hexdigest": "daddee63f10a648e665742e1012dd38b", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_M4S1T5", "query_cov": 0.8809523809523809, "subject_cov": 0.8043478260869565, "identity": 0.649, "score": 54.7, "evalue": 3.78e-09, "valid": false, "product": "Seryl-tRNA(Ser/Sec) synthetase", "uniref50_id": "UniRef50_M4S1T5" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4515.254900000001, "isoelectric_point": 5.97095890045166 }, "id": "GLJLFKCOHC_974", "locus": "GCA013368035_00973" }, { "type": "cds", "sequence": "contig_1", "start": 562291, "stop": 562407, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTAAATGATGCCAAAAGTATTCTTGAATTGCTTGAAATTCCATACCGTGAAATGATGCTTTCAACTGGCGATATTGGATTTAGCTCAGCAAAAACTATTGACCTAGAACTATGA", "aa": "MVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLEL", "aa_hexdigest": "57b07484f1a654554c468177557b5823", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4244.898999999999, "isoelectric_point": 4.196388816833497 }, "id": "GLJLFKCOHC_975", "locus": "GCA013368035_00974" }, { "type": "cds", "sequence": "contig_1", "start": 562414, "stop": 562569, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00587.30" ], "nt": "TTGTCTAAGCCAAAGAAATTTGTTGCATATACAAAATGTTTCCGTTCAGAAGCAGGAAGCAGTGGAAAAGATACAAAAGGTTTAATTAGACAACACGAATTCCATAAAGTCGAACTTGTAAAAATAACCAGTGAAAAAGATGCAATGAAAGAATGA", "aa": "MSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKE", "aa_hexdigest": "1bf5c3511f53ff80841e97b1b2e2591e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00587.30", "name": "tRNA-synt_2b", "length": 46, "aa_cov": 0.9019607843137255, "hmm_cov": 0.9130434782608695, "evalue": 4.021530245260099e-11, "score": 38.68495178222656, "start": 4, "stop": 49 } ], "seq_stats": { "molecular_weight": 5830.716199999999, "isoelectric_point": 9.61141414642334 }, "id": "GLJLFKCOHC_976", "locus": "GCA013368035_00975" }, { "type": "cds", "sequence": "contig_1", "start": 562627, "stop": 563307, "strand": "-", "frame": 2, "gene": "serS", "product": "serine--tRNA ligase", "db_xrefs": [ "COG:COG0172", "COG:J", "EC:6.1.1.11", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434", "KEGG:K01875", "SO:0001217", "UniRef:UniRef50_Q98RH5", "UniRef:UniRef90_D1J8K7" ], "nt": "ATGTTAGATTTAAAATATATTATTAGTAACAAAAATGAAGTTATTAAAAAACTATCAACACGTAATTTCAATATTGCTATTATCGAAGAAATTTTTGAAGACGCTCAAAAAAGAAACGAATTAATTCATAATTTAGAAACTTTGCAAGCTAAAAGAAATAAATTAAGTGAACAAATTGGAATTAATAAAAGAAACAAGCAAGATTGCTCGGAATTATTACAACAAGTTGCTGAAGTTAAAGAACAAATTGAAAAAATTGATGAAGAAGCTGACACATTGGTAAAGAAAGTCAATACATTACTATACCAAATTCCTAACTTGCCATATGATGAAGTTCCTGTGGGAAGAGATGAAGATGACAACGTAGTTTTAAAAGAACATCCAAATTTAGGGAGAGGGTTAGTAAAAGGCGTATTGCCACATTATGAAATAGCAACAAAATTAGATATTGTTGATTTTGTTAGATCAGTGAAGTTAGCAAGCACAAGATTTGTTTTATACAAAAATGATGGTGCTAAATTAGTAAGATCCTTAGCCTCTTTTATGCTTGATACGCACATTGCAAATGGATATAAAGAATTAATGCCTGCCCATTTAGTTAATTCAAATATGCTTTATGGAACCGGGCAATTGCCTAAATTTGCAGAAGATTTATTTAAAATGCAAGGCAATGACCTTTGA", "aa": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDL", "aa_hexdigest": "799214d340c25b0e645f110351528f7b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "serS" ], "truncated": "5-prime", "pseudogene": { "start": 562324, "stop": 563307, "observations": { "mutation": [ 562416, 562629 ], "stop": [ 562416, 562629 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K7", "query_cov": 1.0, "subject_cov": 0.5368171021377672, "identity": 1.0, "score": 376.0, "evalue": 8.29e-128, "gene_start": 1, "gene_end": 226, "reference_start": 1, "reference_end": 226, "reference_sequence": "MLDLKYIISNKNEVIKKLSTRNFNIAIIEEIFEDAQKRNELIHNLETLQAKRNKLSEQIGINKRNKQDCSELLQQVAEVKEQIEKIDEEADTLVKKVNTLLYQIPNLPYDEVPVGRDEDDNVVLKEHPNLGRGLVKGVLPHYEIATKLDIVDFVRSVKLASTRFVLYKNDGAKLVRSLASFMLDTHIANGYKELMPAHLVNSNMLYGTGQLPKFAEDLFKMQGNDLWLIPTAEVPVTNYYYDEILDLSKPKKFVAYTKCFRSEAGSSGKDTKGLIRQHEFHKVELVKITSEKDAMKEWESMVNDAKSILELLEIPYREMMLSTGDIGFSSAKTIDLELWIPSEQRYRETSSISICRDFQGRRAKIRYRDETGHTKYAYTMNGSGLAIDRVVAAILENYQNPDGSITIPKVLVPYMGKEVIK" }, "paralog": false, "identity": 0.9938837920489296, "score": 575.0, "evalue": 7.73e-209, "description": "Internal stop codon at 562416, 562629. Nonsense mutation around 562416, 562629.", "psc": { "uniref90_id": "UniRef90_D1J8K7", "gene": "serS", "product": "serine--tRNA ligase", "ec_ids": [ "6.1.1.11" ], "uniref50_id": "UniRef50_Q98RH5", "cog_id": "COG0172", "cog_category": "J", "kegg_orthology_id": "K01875", "go_ids": [ "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K7", "EC:6.1.1.11", "UniRef:UniRef50_Q98RH5", "COG:COG0172", "COG:J", "KEGG:K01875", "GO:0000166", "GO:0004828", "GO:0005524", "GO:0006434" ] }, "pscc": { "uniref50_id": "UniRef50_Q98RH5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98RH5" ], "product": "Serine--tRNA ligase" } }, "id": "GLJLFKCOHC_977", "locus": "GCA013368035_00976" }, { "type": "cds", "sequence": "contig_1", "start": 563580, "stop": 563933, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATCCTGTTAAAGTTAATAAATACAACAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACCTTAAACAGCCACATTTATGAAATATCAGTCTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAACTATGTTATTAAATTTTTAAAAAGTAGTCATTTTCCTGGTATAGGCGAAGAAAAAGCAAAATTAATTGTAAAAGATTTAGGGATCGATGCTTTAAGAAAAATGGTTGAGGATAATTCAATTGATTATTCAAAATATTCAATATCAGAATTGACATGA", "aa": "MNPVKVNKYNSISVTLKNNLFSEKNITLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELT", "aa_hexdigest": "2f68b14a209bcc58011a83bcb9139939", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13419.083699999985, "isoelectric_point": 6.111578559875488 }, "id": "GLJLFKCOHC_978", "locus": "GCA013368035_00977" }, { "type": "cds", "sequence": "contig_1", "start": 563985, "stop": 564296, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTTTTAACAAATAATTTAACGCCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTATAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGTATCTCACTTGAAGATATGGACAAATTACATAATATCTTTAACAAATTTGAACACCCCTTTAAACCTGCAACCCACTTGTACAAATCATTTTTAAAATATCTATATAATTCTGGAAACACTAGAATTTTAAAAGATTATTTATACGACCAACTAATAAACTTTGCAAGCCAAGACACTGAATGA", "aa": "MFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTE", "aa_hexdigest": "bbd10f8aa144e225834635c5cbecca17", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12574.095599999982, "isoelectric_point": 5.754743385314941 }, "id": "GLJLFKCOHC_979", "locus": "GCA013368035_00978" }, { "type": "cds", "sequence": "contig_1", "start": 564336, "stop": 565718, "strand": "+", "frame": 3, "gene": "recD", "product": "Exodeoxyribonuclease V alpha chain", "db_xrefs": [ "COG:COG0507", "COG:L", "SO:0001217", "UniRef:UniRef50_D1J8K8", "UniRef:UniRef90_D1J8K8" ], "nt": "TTGGTTCAAAATAAATATTTAATCGAAATTGAATACGAAGATGGTACATATCTTAGTGCTAAAGATGTATTTGATATGGAAAAATATATTGTCAAAAGATTAAAACATATTGAATCTTCTTCAAACCCAAAATTCATTAATTTCAAGCCAAGCGAAATGTTTCACCCTTTGCAACTTCAAGCAATTGAAACAGCACTAACTAGCAAATTGACATTGATTACAGGAAACCCCGGCACTGGAAAAACATTGATAACTAATGAAATAATTAAACAATTGCTTTCAGTCTATGATGAAGATGATTTGGCAATAGTCACACCAACAGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAAACAAATGCAGTTACTATACATAGTTTTTTAGAATGGGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTTGATTTGTTTTATTCACTACTTAAAGGAATAAAAAAGAATTCATTAAAACGAATCATATTAGTTGGTGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGATGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCTCAGTTTATTGAAACGAAACAGAGTGACCTAAAGTCAATGTTACTCTTTAAATTAAATGACTTATTGCAACAAGGCTATAGCAAGCGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGATCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAGTATCGAGACCATGTTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCTTTTGAAAAAGTTTTGGCAAGTATCTCTGTTGAATTTGAAAATGATTCAAAAACTGTCTCTTATTCTAGAAAAGATTTTTTTGCTAATACATACCCTGCATATTGCACAAGCGTTCATAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCCAAAAAACTTATTTATACTGCTATTACTAGAGCAAAAAAATATTGCGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAATGAATAA", "aa": "MVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESSSNPKFINFKPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHVLAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVEFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE", "aa_hexdigest": "175dd8e5c69ce42333113355dfbf52c4", "start_type": "TTG", "rbs_motif": null, "genes": [ "recD" ], "truncated": "5-prime", "pseudogene": { "start": 564036, "stop": 565718, "observations": { "mutation": [ 564294 ], "stop": [ 564294 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8K8", "query_cov": 1.0, "subject_cov": 0.6157965194109772, "identity": 0.991, "score": 899.0, "evalue": 0.0, "gene_start": 1, "gene_end": 460, "reference_start": 288, "reference_end": 747, "reference_sequence": "MLQNSNIQGKFNKIMWTSTDGQVVIVSFLVSSNDGLNPVKVNKYNSISVTLKNNLFSEKNVTLNSHIYEISVSKNSFSKYPNSYIATDVIEINEDEEYKLNYVIKFLKSSHFPGIGEEKAKLIVKDLGIDALRKMVEDNSIDYSKYSISELTWNYAVEFIKNNPTLIEDQMFFLTNNLTPSLYEIVAKKFEHFKDFIDAYKDNFYLFYLDNERISLEDMDKLHNIFNKFEHPFKPATHLYKSFLKYLYNSGNTRILKDYLYDQLINFASQDTEWPRDVETFNNSVELLVQNKYLIEIEYEDGTYLSAKDVFDMEKYIVKRLKHIESSSNPKFINFMPSEMFHPLQLQAIETALTSKLTLITGNPGTGKTLITNEIIKQLLSVYDEDDLAIVTPTGRATININNIQEKTNAVTIHSFLEWDVDNDKFMINEKNAKPIECLIIDEFSMVSVDLFYSLLKGIKKNSLKRIILVGDKDQLPAIGAGYLIRDFIESNIFQTIVLSKIFRQAENFDIIQDALDINKGKLPEFKGNNSQFIETKQSDLKSMLLFKLNDLLQQGYSKRDIAILSPIYNYETGIDQINETLNNFFRKLDGNEQIKYRDHILAIDDKVINLVNDPKTKVFNGEIGYISHFGFENKKNSFEKVLASISVDFENDSKTVSYSRKDFFANTYPAYCTSVHKYQGSECKCVIVVLFSEAKKLLSKKLIYTAITRAKKYCVVIGEKEALITGINNDDDSNRITNIKYLWRNE" }, "paralog": false, "identity": 0.9928571428571429, "score": 1111.0, "evalue": 0.0, "description": "Internal stop codon at 564294. Nonsense mutation around 564294.", "psc": { "uniref90_id": "UniRef90_D1J8K8", "gene": "RecD", "product": "Exodeoxyribonuclease V alpha chain", "uniref50_id": "UniRef50_D1J8K8", "cog_id": "COG0507", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8K8", "UniRef:UniRef50_D1J8K8", "COG:COG0507", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8K8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8K8" ], "product": "Exodeoxyribonuclease V alpha chain" } }, "id": "GLJLFKCOHC_980", "locus": "GCA013368035_00979" }, { "type": "cds", "sequence": "contig_1", "start": 565718, "stop": 566278, "strand": "+", "frame": 2, "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "db_xrefs": [ "COG:COG0193", "COG:J", "EC:3.1.1.29", "GO:0004045", "RefSeq:WP_012855695.1", "SO:0001217", "UniParc:UPI0001BC2435", "UniRef:UniRef100_D1J8K9", "UniRef:UniRef50_Q98PE2", "UniRef:UniRef90_D1J8K9" ], "nt": "ATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCACAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATAGATAAAGAAGTAATTTTAGCCAAACCATTAACCTTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTGCCAGTTGGAAAAGCAGTTATTAGACAAAAAGGTTCGTCTGGCGGACATAACGGAATGAAAGATATAATTGCTAAATTAAAAACCGATGAAATAAAAAGATTAAAAATAGGAATTGGACGTGGTCAAAATGTAATTAATTATGTTCTAGGCAAATTCAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGATATTGCTTCTGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAA", "aa": "MKLIVGLGNPGVEYEKTRHNTGFMVIDKLAEKLHTPLTEKKFNGIYYIDKEVILAKPLTFMNNSGDFVRAIKDYYNIVDDDIIIVYDDLDLPVGKAVIRQKGSSGGHNGMKDIIAKLKTDEIKRLKIGIGRGQNVINYVLGKFSYQDFCTIEKIIDIASDALISFISNDIRFVMNKFSGELYRENK", "aa_hexdigest": "ffa6914d0b626ec4bf14b6ed375ee7a8", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI0001BC2435", "ncbi_nrp_id": "WP_012855695.1", "uniref100_id": "UniRef100_D1J8K9", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC2435", "RefSeq:WP_012855695.1", "UniRef:UniRef100_D1J8K9" ] }, "ips": { "uniref100_id": "UniRef100_D1J8K9", "uniref90_id": "UniRef90_D1J8K9" }, "psc": { "uniref90_id": "UniRef90_D1J8K9", "gene": "pth", "product": "aminoacyl-tRNA hydrolase", "ec_ids": [ "3.1.1.29" ], "uniref50_id": "UniRef50_Q98PE2", "cog_id": "COG0193", "cog_category": "J", "go_ids": [ "GO:0004045" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PE2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PE2" ], "product": "Peptidyl-tRNA hydrolase" }, "genes": [ "pth" ], "id": "GLJLFKCOHC_981", "locus": "GCA013368035_00980" }, { "type": "cds", "sequence": "contig_1", "start": 566394, "stop": 566762, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTAAATCATTGAATATTGTTGTTGCACATGTAAACTACAACAAAAGATATGATAGCAATTATGATGAATCATTAGTTTCACGATTTTGTGCTGATAACAATATCCCTTTAGAAATACTTTCATTACATAACAAATATTCTAGTGGCAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAGTTTCTAAAAAATATCAATGCGACTATATCCTCCTTGCCCACCAAAAAGACGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCATTATTTTGGGGTATTAAGCAACTTAGCATACTAAATAATAACGTCATTTTTAGACCAATGTTAAATTTATGA", "aa": "MLKSLNIVVAHVNYNKRYDSNYDESLVSRFCADNNIPLEILSLHNKYSSGNFQAIARKERYDFYFEVSKKYQCDYILLAHQKDDFLESSIMQENSNRKPLFWGIKQLSILNNNVIFRPMLNL", "aa_hexdigest": "538d81f4bc277f225a864fef1b8c2bf8", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14463.338299999987, "isoelectric_point": 8.517385673522949 }, "id": "GLJLFKCOHC_982", "locus": "GCA013368035_00981" }, { "type": "cds", "sequence": "contig_1", "start": 567248, "stop": 567457, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAATCCAAAAGAAAAAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTTATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAATGTTATTAGAGGAACTGCTAACAAAAATGATGGAAACTATATTTCTTCATGA", "aa": "MNNPKEKKPRKIGLFGIIGILFAIIAVITLGYIISDVVKERMSNFQTLNFLENVIRGTANKNDGNYISS", "aa_hexdigest": "7b75372bc2f7ddd2b075f58d089ff3ee", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7699.945, "isoelectric_point": 9.77445468902588 }, "id": "GLJLFKCOHC_983", "locus": "GCA013368035_00982" }, { "type": "cds", "sequence": "contig_1", "start": 567656, "stop": 568957, "strand": "+", "frame": 2, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "nt": "TTGAATTCTTGTGCAACATTTAATGGTCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAACCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTTGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGCAAGGGAAGAGGAATGCTAACTGGCGGTATTGATACAAACAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCTGGCGCTAGAATTCCAAAAGGAATATTACTAGGAGGCCCCCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAACGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTAGGTGCAAAAAGAGTTCGTGACATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGATGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGACGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAGATGGATGGTATCAAAGAAAATAGTGGTATTTTAATAATGGCAGCTACAAATAGAACCGATGTTCTAGACCCTGCTTTATTAAGACCAGGACGTTTTGATAGAACTATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATTTTAAAATTACATGCTAATGGAAAAAGAATTGCAAGCAATATTAATTTTGCTATGGTAGCAAGAAGAACACCTGGTTTCTCTGGAGCACAGCTTGAAAATGTAATTAACGAAGCCTCTTTATTAAGTGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACCATTTCTGAAAAAGAAAACGTTGCAGTTGCCTACCATGAAGCGGGACATGCAGTTGTTGGAATAAAAATTAAAGGTGGAAATAAAGTTCAAAAAATAACTATCATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCAGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCATTCATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGACGATATTGCTAAGGCAACAAATATTGCTAGAAAAATGGTTACTGAATGA", "aa": "MNSCATFNGQVLGNIVASSMPKPSIASKYIIPAFSAFVGLAFIIIIMAMYFGKGRGMLTGGIDTNKSLAQRIYSNKKFSDIAGNEEVKEEVKELVDYLKNPKKYEAAGARIPKGILLGGPPGTGKTLIAKATAGEANVPFFFISASNFVEMFVGLGAKRVRDMFAEARSHAPAIIFIDELDAVGRSRGAGIGGGNDEREQTLNQLLVEMDGIKENSGILIMAATNRTDVLDPALLRPGRFDRTITVGLPDIKEREAILKLHANGKRIASNINFAMVARRTPGFSGAQLENVINEASLLSVRENTGVITIEQIDEAIDRVIAGPAKKSRTISEKENVAVAYHEAGHAVVGIKIKGGNKVQKITIIPRGKAGGYNLMMPDEEKYNRSKSELLAIITSFMGGRVAEAIIYGADNVSTGASDDIAKATNIARKMVTE", "aa_hexdigest": "c989c8a2f48855be8e52fd3f7dc7ad78", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A485JC26", "query_cov": 0.9468822170900693, "subject_cov": 0.8102766798418972, "identity": 0.512, "score": 404.0, "evalue": 4.01e-134, "valid": false, "gene": "ftsH", "product": "ATP-dependent zinc metalloprotease FtsH", "ec_ids": [ "3.4.24.-" ], "uniref50_id": "UniRef50_A0A485JC26", "go_ids": [ "GO:0004176", "GO:0004222", "GO:0006508" ] }, "pscc": { "uniref50_id": "UniRef50_A0A485JC26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A485JC26" ], "product": "ATP-dependent zinc metalloprotease FtsH" }, "genes": [ "ftsH" ], "id": "GLJLFKCOHC_984", "locus": "GCA013368035_00983" }, { "type": "cds", "sequence": "contig_1", "start": 569027, "stop": 569467, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01434.24" ], "nt": "ATGAAAAATTCTTCATTTTCAAGTAAAGTAGGCCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACTGCTGAAAAGAAAGCACATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTCGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTACCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAATGGTTCAAGCAACCATTCAGAAGATTCTAATGATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAA", "aa": "MKNSSFSSKVGQEIDEQIRKIILTAEKKAHEIITENRQLLELIKDALIIKETIVAEEIEYIEKHMELPPEITNTKKELKNEYSEEDFNELFNEVAGQKQIQEDKYKATLNSELKKHNIEDNGSSNHSEDSNDTKNSDEDSNKEDNQ", "aa_hexdigest": "61bd94cd32eb25b9076ad18ed4e27bf0", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01434.24", "name": "Peptidase_M41", "length": 59, "aa_cov": 0.4041095890410959, "hmm_cov": 1.0, "evalue": 7.375265033194668e-11, "score": 37.650604248046875, "start": 2, "stop": 60 } ], "seq_stats": { "molecular_weight": 16923.382699999987, "isoelectric_point": 4.682134819030761 }, "id": "GLJLFKCOHC_985", "locus": "GCA013368035_00984" }, { "type": "cds", "sequence": "contig_1", "start": 569493, "stop": 569846, "strand": "-", "frame": 3, "gene": "ygaC", "product": "DUF402 domain-containing protein", "db_xrefs": [ "COG:COG3557", "COG:S", "KEGG:K07586", "SO:0001217", "UniRef:UniRef50_A0AA43TZF8", "UniRef:UniRef90_D1J8L2" ], "nt": "TTGTTTTTCTTTGCTAGAAACAAATTTTATAATGCTTCTATTACATTAAATAAATCTAAAGAAAACTATATTTATATTAATTTAGCAAGCCCTTTTTATATTGAAAATGAAGTAATTAAATATATTGATTTTGATATCGATATTAAAAGTTATGATGATAAAGATTTTAGCGTTATAGATTGGCAAGATTTTAAAAATTCAATTAGTCAATACAATTATTCATTGAAATTAATTTATAAATTGTATGAAGAATTAGATTTTTTATATGAACAAAAGCAAAAAGGAACAGGAGTTTTTTCTAAGAAACTTGTTAACGGCCTTGAAAAGATGTTGCGAGAAAGCGGAGATATATAA", "aa": "MFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI", "aa_hexdigest": "3b42402dbf2f9f440d25f246ec1fd3a3", "start_type": "TTG", "rbs_motif": null, "genes": [ "ygaC" ], "truncated": "3-prime", "pseudogene": { "start": 569493, "stop": 570107, "observations": { "mutation": [ 569867 ], "stop": [ 569867 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L2", "query_cov": 1.0, "subject_cov": 0.5735294117647058, "identity": 0.991, "score": 228.0, "evalue": 2.52e-74, "gene_start": 1, "gene_end": 117, "reference_start": 88, "reference_end": 204, "reference_sequence": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLVKTKVSEKSINWVVSEPILFFFARNKFYNASITLNKSKENYIYINLASPFYIENEVIKYIDFDIDIKSYDDKDFSVIDWQDFKNSISQYNYSLKLIYKLYEELDFLYEQKQKGTGVFSKKLVNGLEKMLRESGDI" }, "paralog": false, "identity": 0.9901960784313726, "score": 401.0, "evalue": 3.9e-145, "description": "Internal stop codon at 569867. Nonsense mutation around 569867.", "psc": { "uniref90_id": "UniRef90_D1J8L2", "gene": "YgaC", "product": "DUF402 domain-containing protein", "uniref50_id": "UniRef50_A0AA43TZF8", "cog_id": "COG3557", "cog_category": "S", "kegg_orthology_id": "K07586", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L2", "UniRef:UniRef50_A0AA43TZF8", "COG:COG3557", "COG:S", "KEGG:K07586" ] }, "pscc": { "uniref50_id": "UniRef50_A0AA43TZF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0AA43TZF8" ], "product": "DUF402 domain-containing protein" } }, "id": "GLJLFKCOHC_986", "locus": "GCA013368035_00985" }, { "type": "cds", "sequence": "contig_1", "start": 569865, "stop": 570107, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAGAAGAAAGTATAAAGAGTTTTGATGATTTATTAAAAACAATTGATACACCAAACTCAAAAAATATAAAAAAACATAAAAAGTTTTTTAAGATGGGACAACTAACAACCATTCAAGTTTTTAAATATGATGGAACTTTATACAGACAATATGAAGGGGCAAAAATAGTTGCAAATTTAACTGACTTTGTAGTTGTTTTGTTAATGAAAACAAAAGTTTCTGAAAAATCTATCAATTGA", "aa": "MEEESIKSFDDLLKTIDTPNSKNIKKHKKFFKMGQLTTIQVFKYDGTLYRQYEGAKIVANLTDFVVVLLMKTKVSEKSIN", "aa_hexdigest": "94f70607d96c17d2aa44eef453fc226f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9277.7353, "isoelectric_point": 9.363597679138184 }, "id": "GLJLFKCOHC_987", "locus": "GCA013368035_00986" }, { "type": "cds", "sequence": "contig_1", "start": 570361, "stop": 570627, "strand": "+", "frame": 1, "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "db_xrefs": [ "COG:COG0220", "COG:J", "EC:2.1.1.33", "GO:0006400", "GO:0008176", "GO:0043527", "SO:0001217", "UniRef:UniRef50_Q9F411", "UniRef:UniRef90_Q9F411" ], "nt": "ATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAAAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAAAGCATCCTGACATAGAATATATTGGACTTGAAAAATACAGCACGCCAGCTTATTCTGCTTTAAAAAAGGCTATTGACCTCAACCTTGAAAATTTTCACATAATTATAGGAGACGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGA", "aa": "MASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALKHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTI", "aa_hexdigest": "0853375c7acf98a4f16d0bdfcb0617fe", "start_type": "ATG", "rbs_motif": null, "genes": [ "trmB" ], "truncated": "3-prime", "pseudogene": { "start": 570322, "stop": 570930, "observations": { "mutation": [ 570625, 570646 ], "stop": [ 570625, 570646 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9F411", "query_cov": 1.0, "subject_cov": 0.43564356435643564, "identity": 0.9890000000000001, "score": 176.0, "evalue": 1.71e-54, "gene_start": 1, "gene_end": 88, "reference_start": 14, "reference_end": 101, "reference_sequence": "MRLRFNKNAETSLMASPMTFKDFPIDNKKNTILEIGMGRGTMLTKLALMHPDIEYIGLEKYSTPAYSALKKAIDLNLENFHIIIGDAINLSTYFKNKIKTIWLTFSDPWPKKRHYKRRLVYRDFLKIYQNVLDKDGVVYFKTDNDMLYQFAIDELKEINAKIIYQTSDLHHCNFKIENVFTDYEEKFNKLNKNINFIAFTFN" }, "paralog": false, "identity": 0.9752475247524752, "score": 399.0, "evalue": 4.83e-145, "description": "Internal stop codon at 570625, 570646. Nonsense mutation around 570625, 570646.", "psc": { "uniref90_id": "UniRef90_Q9F411", "gene": "trmB", "product": "tRNA (guanosine(46)-N7)-methyltransferase TrmB", "ec_ids": [ "2.1.1.33" ], "uniref50_id": "UniRef50_Q9F411", "cog_id": "COG0220", "cog_category": "J", "go_ids": [ "GO:0006400", "GO:0008176", "GO:0043527" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9F411", "EC:2.1.1.33", "UniRef:UniRef50_Q9F411", "COG:COG0220", "COG:J", "GO:0006400", "GO:0008176", "GO:0043527" ] }, "pscc": { "uniref50_id": "UniRef50_Q9F411", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q9F411" ], "product": "tRNA (guanine-N(7)-)-methyltransferase" } }, "id": "GLJLFKCOHC_988", "locus": "GCA013368035_00987" }, { "type": "cds", "sequence": "contig_1", "start": 570757, "stop": 570930, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATTAATGCAAAAATCATTTATCAAACGTCTGATTTACATCATTGCAATTTTGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCATTTACTTTCAATTAA", "aa": "MLYQFAIDELKEINAKIIYQTSDLHHCNFEIENVFTDYEEKFNKLNKNINFIAFTFN", "aa_hexdigest": "b45279f89f0fa34c5eb3b40da5a6eb1e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6923.743199999997, "isoelectric_point": 4.855323219299316 }, "id": "GLJLFKCOHC_989", "locus": "GCA013368035_00988" }, { "type": "cds", "sequence": "contig_1", "start": 571005, "stop": 572678, "strand": "+", "frame": 3, "gene": "gyrA", "product": "DNA gyrase subunit A", "db_xrefs": [ "COG:COG0188", "COG:L", "EC:5.6.2.2", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265", "KEGG:K02469", "SO:0001217", "UniRef:UniRef50_B3PN30", "UniRef:UniRef90_D1J8L4" ], "nt": "ATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAATAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGATGAAGAAGACGAAGAAGAAATTCCTCAAGACAAAGAAGGCTATAAAGTAGAACCCCAACTTTTAGATAAAGAAATTAATGGTTTAAAGCCTGCCAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCTCGTGCTCTCCCTGATGCTCGTGATGGACTAAAGCCGGTGCACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCGGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCTTTAATTGATGGACATGGTAACTTTGGATCTGTTGACGGAGACGAAGCTGCTGCAATGCGTTATACAGAAGCTAGAATGAGCAAGATTGCTGGTGCAATGGTTGATGGTATTAAGAAAAATACTGTTGACTTTATGGACAACTATGACGCAACTGAAAAAGAACCAGTTGTTTTGCCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTGGGCGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTAATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATCATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCGAACAGAGATGGCATTAGAATTGTAATTGATATCAAAAAATCTTTTGTTCCAGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGTTGGTAAATAATGAACCAAAATTATTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGATTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTTTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCGAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTTAGTGAAATACAAACAAAAGCAATTGTTGATATGAAGCTAGGAAGATTAACTGGATTAGCAATTGAAAAAATGAACGAAGAACTAAATGAAGTACATGAAAGAATTGCTAACTACATTAAGATATTAGGCGACCATAACTTATTAATCAATTTGATAATTGAAGAATTACAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGA", "aa": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEEIPQDKEGYKVEPQLLDKEINGLKPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIKILGDHNLLINLIIEELQEIKNAYGDARRSDIN", "aa_hexdigest": "5f7f774c368641cfdc22ec26a160be3f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0015AA695A", "query_cov": 0.8725314183123878, "subject_cov": 0.8983364140480592, "identity": 0.595, "score": 575.0, "evalue": 3.79e-199, "valid": false, "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_A0A2N1QA61" }, "genes": [ "gyrA" ], "truncated": "3-prime", "pseudogene": { "start": 571005, "stop": 572981, "observations": { "mutation": [ 572676 ], "stop": [ 572676 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L4", "query_cov": 1.0053859964093357, "subject_cov": 0.612691466083151, "identity": 0.986, "score": 1051.0, "evalue": 0.0, "gene_start": 1, "gene_end": 557, "reference_start": 1, "reference_end": 560, "reference_sequence": "MLFGEDDNNKKENKNFEEDNSHDLDEYTNFDDRIKKIFSDDDETKAKKIDEEDEEDEEEIPQDKEGYKVEPQLLDKEINGLKPANLSKVMKTSFIEYAMSVIVSRALPDARDGLKPVHRRILYGMSELGMFYTAPHKKSARIVGDVLGKYHPHGDSSVYEAMVRMAQDFSLRYPLIDGHGNFGSVDGDEAAAMRYTEARMSKIAGAMVDGIKKNTVDFMDNYDATEKEPVVLPSRFPNLLVSGSSGIAVGMATNIPPHNLGETIDAVCALAKNPEINVFELMEYIQAPDFPTGGIIFNKKGLIEAYSTGRGSITIRSKAHIQELSNGKSKIIVTEIPYEVKKTEIMEKISELIKDKKIDGIADFRDESNRDGIRIVIDIKKSFVPEIVLNNLFKLTKLQSNYSFNMIALVNNEPKLLNLKACLEVYLKHQIEVVSRRLKFDLEKDRARAHILEGLKICIENIDAVIKIIKESKTDQEAQSKLSQAFNLSEIQTKAIVDMKLGRLTGLAIEKMNEELNEVHERIANYIRILGDHNLLINLIIEELQEIKNAYGDARRSDINWDELSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKLDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE" }, "paralog": false, "identity": 0.9863842662632375, "score": 1272.0, "evalue": 0.0, "description": "Internal stop codon at 572676. Nonsense mutation around 572676.", "psc": { "uniref90_id": "UniRef90_D1J8L4", "gene": "gyrA", "product": "DNA gyrase subunit A", "ec_ids": [ "5.6.2.2" ], "uniref50_id": "UniRef50_B3PN30", "cog_id": "COG0188", "cog_category": "L", "kegg_orthology_id": "K02469", "go_ids": [ "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L4", "EC:5.6.2.2", "UniRef:UniRef50_B3PN30", "COG:COG0188", "COG:L", "KEGG:K02469", "GO:0003677", "GO:0003916", "GO:0003918", "GO:0005524", "GO:0006259", "GO:0006265" ] }, "pscc": { "uniref50_id": "UniRef50_B3PN30", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PN30" ], "product": "DNA gyrase subunit A" } }, "id": "GLJLFKCOHC_990", "locus": "GCA013368035_00989" }, { "type": "cds", "sequence": "contig_1", "start": 572685, "stop": 573740, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF03989.18" ], "nt": "TTGAGCAGCATTGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACATTAACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTTGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTAATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGTCACGAAGTTCCTGTTGGCACCAAACAATCAAAGGGTATTCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAAATTTTGCCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACAATTTTATTTGAAAGTATTAACAGAAACGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTTAAAGACGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGATCAATGGGAAGAATGGCCTATGGTGTTAAGGGAATTAAAATTGATGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTCGGAAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACAAGACGTGGGGCAAGCGGGGTTTATTCATTAAATGAAGAAAAATCTGGCAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTGATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAGCATTGTTGCGGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCTAAAAATAACCTTAATCAAAAAAATGAATAA", "aa": "MSSIDNEDLIPKRDIVITLTNNGYIKRLDIEEYREQRRGGVGVSTTKTYKDDAIQDILVANTHTDLLIFTDLAKVYRIRGHEVPVGTKQSKGIPIVNVIPSIQKNEKVIKILPINEYKQNEYLLTATKKGIVKKTPTILFESINRNGKIAFAIRDDDDKLIDALIVKDEDEIFIAASNNKICRFDSNEIRSMGRMAYGVKGIKIDDVEDNVVSVSSSNQGKYIFSLGKEGYGKLTLAAMFRKTRRGASGVYSLNEEKSGKLIYAATVHGVEDLIVLTSDGVAIRFSLKDVAITGRNTKGVKLVNLKGRTTSIVAATKILDDDLEDNDRKLSENEFKEIESDAKNNLNQKNE", "aa_hexdigest": "55d88bc43c480ce905e50f0f43fba182", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF03989.18", "name": "DNA_gyraseA_C", "length": 48, "aa_cov": 0.13675213675213677, "hmm_cov": 1.0, "evalue": 4.7373261664878894e-57, "score": 184.67364501953125, "start": 14, "stop": 61 } ], "seq_stats": { "molecular_weight": 39147.20080000002, "isoelectric_point": 8.587204933166507 }, "id": "GLJLFKCOHC_991", "locus": "GCA013368035_00990" }, { "type": "cds", "sequence": "contig_1", "start": 574114, "stop": 574215, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCAGAATCGATCCAAATAAATCAATATACAAAGAAGTTAATGATACTATAAAACGCTTAGAACAAAGTGGAAAATATGCCTCAAAAATTTCTGAATGA", "aa": "MTRIDPNKSIYKEVNDTIKRLEQSGKYASKISE", "aa_hexdigest": "fa61966352c9a8058a6181d12f21f3dc", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3843.3222000000005, "isoelectric_point": 9.223185539245605 }, "id": "GLJLFKCOHC_992", "locus": "GCA013368035_00991" }, { "type": "cds", "sequence": "contig_1", "start": 574582, "stop": 574830, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCAAAAATTTAATAAGCTAGATAGTGGTGTAAAAGATCCAGATTTTTTAGGTGTAACACAACAACTAAATGAAATAAAAGAAAAGGCATTGCAAGAATTAGCAAATATTAAAAACAATTCTAGTCAATTGGCAACCATTGATGAAGCGTCAAAATATGAAAATAATATACAAGAAGTACAAGAAAAATTTGTTAAAGATTTAATAAAAATATTTGATGGAAATATAAATACAGATAGGATTAATTGA", "aa": "MQKFNKLDSGVKDPDFLGVTQQLNEIKEKALQELANIKNNSSQLATIDEASKYENNIQEVQEKFVKDLIKIFDGNINTDRIN", "aa_hexdigest": "8f13256596a17dbd7ce0c67541b8915d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9384.418199999991, "isoelectric_point": 4.848388862609862 }, "id": "GLJLFKCOHC_993", "locus": "GCA013368035_00992" }, { "type": "cds", "sequence": "contig_1", "start": 574966, "stop": 575898, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_241863256.1", "SO:0001217", "UniParc:UPI001F519C31", "UniRef:UniRef100_UPI001F519C31", "UniRef:UniRef50_UPI001F519C31", "UniRef:UniRef90_UPI001F519C31", "PFAM:PF04778.17" ], "nt": "ATGAACAATAAAAGCAAAAAGATCGTTCTTCCATTAGCAATTTTTTGTGGCGGATTATCAATAGCTGCCATCGCTACCGTAGCAATAAGATCGTGTCAAAATAAAGTGGATAAAAAGACACAAAAAGAATCACTCTTCAAAAAATTTAAAATTCTTCAAGAAGAATTGCAATTATTGCTAGAAAATAAAAACATTCAAGGAATTTATTCAATTGAAGAACAAAATGTCTTTGATAATTCCAAAATGATCAATATTGAATGGCCAATTGATAATATTAATCAAGAAATTTTTAAGATTCAAAGTTCGATTGATTCATTAAATGCAAAAATTCAAAAGCAAAGCATGCAAAAACTACTTTTTGCAGCAAAGAAACAGTTGGAAAATATTATTCATGCTATGAGGATATTGGAAAATAAATTAATTGATAATGATGATGATATTCAAAAAACTAAGGAAGATTTAAATTTGCAAATTATTAATGCATTAGAGGTTTATAACAAAAATAATATAGACGAAATAAATAATTTAAATAAATCATTAACTTTTAAAATTTCTGAAGTTGAAGATATATTGATCAAATTTAAAAAATCAAAAGAACAAGAATTTGAAAAATTACAAAAAACTAGAAAATTAGTTGAAGAATTTTTGGCCCAAGATGTTGTTAAATCTGATTATTATGCAAATATAGTTAGAAATCTTAATAGAGAAGTTTCAAGACGAGGAAACGTTTCAATTAGTTCTAATAAATCAGAAATAGTAGCTGCAAATATTAATTTAAATGAGGTTTTAAAACAAAGTAAAGAATTCGTTGAAAATGATATGCAAAAATTAAGGGATAGCTTAATTTCTTTAATTGTAGAAGCAAAGGAATATATCGAAAATAAAATGCGCCCTAGAGATAAATACAATGCATACATTGAAAGAATAGAATAA", "aa": "MNNKSKKIVLPLAIFCGGLSIAAIATVAIRSCQNKVDKKTQKESLFKKFKILQEELQLLLENKNIQGIYSIEEQNVFDNSKMINIEWPIDNINQEIFKIQSSIDSLNAKIQKQSMQKLLFAAKKQLENIIHAMRILENKLIDNDDDIQKTKEDLNLQIINALEVYNKNNIDEINNLNKSLTFKISEVEDILIKFKKSKEQEFEKLQKTRKLVEEFLAQDVVKSDYYANIVRNLNREVSRRGNVSISSNKSEIVAANINLNEVLKQSKEFVENDMQKLRDSLISLIVEAKEYIENKMRPRDKYNAYIERIE", "aa_hexdigest": "d94bcd47e292671ad79eccd3b16ef6f4", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI001F519C31", "ncbi_nrp_id": "WP_241863256.1", "uniref100_id": "UniRef100_UPI001F519C31", "db_xrefs": [ "SO:0001217", "UniParc:UPI001F519C31", "RefSeq:WP_241863256.1", "UniRef:UniRef100_UPI001F519C31" ] }, "ips": { "uniref100_id": "UniRef100_UPI001F519C31", "uniref90_id": "UniRef90_UPI001F519C31" }, "psc": { "uniref90_id": "UniRef90_UPI001F519C31", "uniref50_id": "UniRef50_UPI001F519C31" }, "pscc": { "uniref50_id": "UniRef50_UPI001F519C31", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F519C31" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF04778.17", "name": "LMP", "length": 154, "aa_cov": 0.4870967741935484, "hmm_cov": 1.0, "evalue": 5.3139858210671714e-55, "score": 181.0630645751953, "start": 135, "stop": 285 } ], "seq_stats": { "molecular_weight": 36086.20200000004, "isoelectric_point": 8.725618553161617 }, "id": "GLJLFKCOHC_994", "locus": "GCA013368035_00993" }, { "type": "cds", "sequence": "contig_1", "start": 576324, "stop": 576512, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAGGTTATAGAAGATATTCAAATAAATGCTAATCCCGCAATAGCAATAGATAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGA", "aa": "MLNKSTTLLFTENFLFFINKVKKVIEDIQINANPAIAIDKTILIGINLVRFTAVSFIGKLLP", "aa_hexdigest": "0a09bd3b6dc0f56311683baa11dc4ddc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6964.3007, "isoelectric_point": 9.69799518585205 }, "id": "GLJLFKCOHC_995", "locus": "GCA013368035_00994" }, { "type": "cds", "sequence": "contig_1", "start": 576546, "stop": 576770, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAACCACTTATTAAAGCAATGAAAACAGGCACTAATGCATATTCAATCATTCATGTGCTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAGTATACCTAAAAAGGCACCTTTTCATGGTCCTAACGCTAAGCCAAAAATAATGAATACTGCATAA", "aa": "MLKKLNPIIHVIFCLAYINLNNQPLIKAMKTGTNAYSIIHVLIPLITWFSVSHSIPKKAPFHGPNAKPKIMNTA", "aa_hexdigest": "acce4a94919d4a8c92664eca63c8e709", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8276.937899999994, "isoelectric_point": 10.187310218811035 }, "id": "GLJLFKCOHC_996", "locus": "GCA013368035_00995" }, { "type": "cds", "sequence": "contig_1", "start": 576728, "stop": 576931, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTTGAATTTATTCAAAAAATTAGACGTATTAAAAAAACAAAACAACATAAATTAACAGTTTTTGAAATTTCTTTATTTGCTTTACTTTTAGCTATTTATATAATTGCTGCTATCTTAGAAAGATTTGTTTTTAAAGGAATAATGAATATTAATATTACTTATGCAGTATTCATTATTTTTGGCTTAGCGTTAGGACCATGA", "aa": "MFEFIQKIRRIKKTKQHKLTVFEISLFALLLAIYIIAAILERFVFKGIMNINITYAVFIIFGLALGP", "aa_hexdigest": "8d8277910469a4365480cd7504f96ef4", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.507999999994, "isoelectric_point": 10.21090564727783 }, "id": "GLJLFKCOHC_997", "locus": "GCA013368035_00996" }, { "type": "cds", "sequence": "contig_1", "start": 577106, "stop": 577333, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTTTTGATTCAGACTATGAAGAATTTATGAAAGGTTCAATTGTTGCGGATTTGAAGAATACAGACTATACTGGACATGCAGGTTCTTGTTCAGCAGCAATGTTCTTAAAAGAATTTACAAAAGGAATTGAATATATTCATTTAGATGTTGCGGGAACTAATGATATTGATGAAAAACCAATGTTTGCAATGACAAAAACATTGACAGAATTAGTGTTGAAATAA", "aa": "MPFDSDYEEFMKGSIVADLKNTDYTGHAGSCSAAMFLKEFTKGIEYIHLDVAGTNDIDEKPMFAMTKTLTELVLK", "aa_hexdigest": "d7f4f7412216ed7607c491ffc4bc2a8d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 59, "aa_cov": 0.7866666666666666, "hmm_cov": 0.9491525423728814, "evalue": 7.558222710275156e-15, "score": 50.34300994873047, "start": 1, "stop": 59 } ], "seq_stats": { "molecular_weight": 8373.472299999996, "isoelectric_point": 4.60369701385498 }, "id": "GLJLFKCOHC_998", "locus": "GCA013368035_00997" }, { "type": "cds", "sequence": "contig_1", "start": 577403, "stop": 578020, "strand": "-", "frame": 1, "gene": null, "product": "M17 family metallopeptidase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "nt": "TTGGGTATTACCCCTCCTAATGAATTAAATTCTGAACAATTAGCTAAAATAGTTGTTGAAGACTTTAAAGAATATAAAAACCTATCAGTTAAAGTTCTTGAAAAGAAAGAAATTGAAAAATTGGGTATGGGATTATTACTATCTGTGAATAGAGGTTCAGTTTATGAACCTAGAGTTGTAATAATTGAATATAAAGGAAATCCTGATTCAAATCAAAAAACAGTTTTAATTGGTAAAGGAATTACCTTTGACTCTGGCGGTTACAACATTAAAACAGGCCGCAACATGTTAGGAATGAAATATGACATGTCAGGTTCTGCAATAGTTGCATCCGTTTTGAAATCTGTTGCCCAATTAAAACCAAAAACAAATGTATCAGCAATTATGTGCATTACTGACAATAGAGTTAATGGAGATGCTTCATTGCCTGATTCAGTTTGGACTTCTATGAGTGGAAAGACTGTTGAAATAAATAATACTGACGCTGAAGGTAGATTAGTTATGGCTGATGGCTTGTGCTATGGAGCTGATGTGTTAAAAGCAACAAGATTAATTGATGTTGCTACATTAACTGGTGCAATGGTTGCAGCTCTAGGAGACACATATACAGGCATTTGA", "aa": "MGITPPNELNSEQLAKIVVEDFKEYKNLSVKVLEKKEIEKLGMGLLLSVNRGSVYEPRVVIIEYKGNPDSNQKTVLIGKGITFDSGGYNIKTGRNMLGMKYDMSGSAIVASVLKSVAQLKPKTNVSAIMCITDNRVNGDASLPDSVWTSMSGKTVEINNTDAEGRLVMADGLCYGADVLKATRLIDVATLTGAMVAALGDTYTGI", "aa_hexdigest": "5697e8edd402b9f93a669342bedaaab2", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI002201A65A", "query_cov": 0.9365853658536586, "subject_cov": 0.8170212765957446, "identity": 0.594, "score": 220.0, "evalue": 2.4e-69, "valid": false, "product": "M17 family metallopeptidase", "uniref50_id": "UniRef50_UPI002201A65A" }, "pscc": { "uniref50_id": "UniRef50_UPI002201A65A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002201A65A" ], "product": "M17 family metallopeptidase" }, "genes": [], "id": "GLJLFKCOHC_999", "locus": "GCA013368035_00998" }, { "type": "cds", "sequence": "contig_1", "start": 578030, "stop": 578479, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAATTAATTAAACAATTATCAACAAAGCGCAATGATCATTTGTTATTGAAAGCAATATTTGAAGATGAAATTTTGCCAGAATTTTTAGTTAAAGAAACAGAAGCAATAACAGATTTTATTGATAAAAAAGTTTCGTATATTTATCTAGGCAAAAAACAAGAATTACAATACAATTCTTTATATTCATTGGCACAAGATTTAGCATTGAATGCTGCCTACGATTATCAAATTGATCTTTCTTCTTTTGTAACAGAAAATTTAACAATTAATGATGTAGTTGATGCATTAACAAAAGGAATAAATTTCTCAGCTGCAAAGTTATACAATAAAAAGACATTTACATATAAAGAAAATCCAAACAACATAGAATTGTTTATAGAACAAGCGTCTCAATCAGTACTTGACTCATTCAATAAAGCAAAAATATTAATTGAATCTCAAAACTGA", "aa": "MQLIKQLSTKRNDHLLLKAIFEDEILPEFLVKETEAITDFIDKKVSYIYLGKKQELQYNSLYSLAQDLALNAAYDYQIDLSSFVTENLTINDVVDALTKGINFSAAKLYNKKTFTYKENPNNIELFIEQASQSVLDSFNKAKILIESQN", "aa_hexdigest": "3c9b75026abbeff3a1f541b35e1c095d", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17127.276999999984, "isoelectric_point": 4.910002326965332 }, "id": "GLJLFKCOHC_1000", "locus": "GCA013368035_00999" }, { "type": "cds", "sequence": "contig_1", "start": 578493, "stop": 578726, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00883.26" ], "nt": "ATGCCTCTACATGATGATTATGATAAACCAAATAAAGAATCTAAAGTAGCTGATCTTAATAATTACAACAACAGTGAAAAATCAGATTGTAATACAGCAGCAATGTTCTTAAAACATTTTACAAATAATGTTGACTATATTCATTGCGATATTGCAGGAACCGCTGACAATAAAGGAGTAGGACTAGGAATTTTAGTTTCTACCTTTGTTGAATTAGCAGCAAATCAAAAATAA", "aa": "MPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK", "aa_hexdigest": "fe1abfe2770c91cf8972a0375caca55b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00883.26", "name": "Peptidase_M17", "length": 58, "aa_cov": 0.7532467532467533, "hmm_cov": 0.9655172413793104, "evalue": 4.7588995774442076e-15, "score": 51.002933502197266, "start": 1, "stop": 58 } ], "seq_stats": { "molecular_weight": 8490.333599999998, "isoelectric_point": 5.05994358062744 }, "id": "GLJLFKCOHC_1001", "locus": "GCA013368035_01000" }, { "type": "cds", "sequence": "contig_1", "start": 578796, "stop": 579857, "strand": "-", "frame": 3, "gene": "pepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "db_xrefs": [ "COG:COG0260", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZXA3", "UniRef:UniRef90_D1J8L9" ], "nt": "ATGAATTCTATTTATTATGAAACTAAACGTAATAAAGATATGCTTTTAACAGCAGTTTATGAAGGCTCAAACGCTAGTTTAAATATTGCCAAGAAAGATAATCATATTACAGAAGATTTTGAAAAAAATGAAGCATTTATTTACATACCAAAAACTGTAGATAACTACTTTAGTTTTTTAAAAGTAGTTGACAAAATTCTAAGTTCAAATAGAAGAAATTATCAAATTAATATTGCTTCTTTTGTTAAAGAAAATGTTGTGTCAACAGAAGAAGTTTTTAGAGCATTTGTATCTAAATTAGCATTTAAAAATGCAAATCTTTATTCAGCAAAAACCAAAGAAAACAAACAAGAAAAGACAATAACATTGTTTTATGCTGATAAATCGTATGAAGAATTAGTTGAAAGATTGTCAATAATTCTTGAAGCAACAAATAAAGCCCGTAGTTTGCAAATGACACCCCCTAATGTTGCAACTAGTGAATATATCGCTAAGGTAGTAAAAGAAGATTTAGAAAATATTCCTGGACTATCAATAAAAGTTTTAGATCGTGATAACATCAAGAAAAATAACATGGGACTTATGTTGGCAGTAAATTCGGGTTCTTCTTATGAACCAAGAGTTTTGATTGCTGAATATAATGGCAATCCTGAAAGTAAAGAAAAATATGTTTATGTAGGAAAAGGAATTACATTTGATACCGGAGGATATAACACCAAAGGATACTACATGGATGGCATGAAATTTGACATGTCTGGTTCTGTTATTGTTGCATATGCAGTTAAAGCAATTGCTCAACTAAAATTAAAAGCAAACGTAGCAGCAATAATGATGATTACAGATAACGCAATAGATACACACCCAACAATGCCTGAATCTGTAATCAAATCAATGAGTGGTTTAACAGTTGAAATTACTGATACTGATGCTGAAGGAAGATTAGTGTTGGCTGATGGTTTATACTACGGTGCCTCGAAATTGAATGCTTCATTATTGATTGATGCCGCAACTTTAACCGGTTCGATGGAAAGAGCATTAGGAAAGACGTATTCAGGAATTTGA", "aa": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFEKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGI", "aa_hexdigest": "05bf4fef855c48fd651185d5b5ef25c1", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "pepB" ], "truncated": "5-prime", "pseudogene": { "start": 578493, "stop": 579857, "observations": { "mutation": [ 578732, 578777, 578798 ], "stop": [ 578732, 578777, 578798 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8L9", "query_cov": 1.0, "subject_cov": 0.7775330396475771, "identity": 0.997, "score": 672.0, "evalue": 3.39e-242, "gene_start": 1, "gene_end": 353, "reference_start": 1, "reference_end": 353, "reference_sequence": "MNSIYYETKRNKDMLLTAVYEGSNASLNIAKKDNHITEDFKKNEAFIYIPKTVDNYFSFLKVVDKILSSNRRNYQINIASFVKENVVSTEEVFRAFVSKLAFKNANLYSAKTKENKQEKTITLFYADKSYEELVERLSIILEATNKARSLQMTPPNVATSEYIAKVVKEDLENIPGLSIKVLDRDNIKKNNMGLMLAVNSGSSYEPRVLIAEYNGNPESKEKYVYVGKGITFDTGGYNTKGYYMDGMKFDMSGSVIVAYAVKAIAQLKLKANVAAIMMITDNAIDTHPTMPESVIKSMSGLTVEITDTDAEGRLVLADGLYYGASKLNASLLIDAATLTGSMERALGKTYSGIWSTSEQRWEDFNKAAKIAHEKVWRMPLHDDYDKPNKESKVADLNNYNNSEKSDCNTAAMFLKHFTNNVDYIHCDIAGTADNKGVGLGILVSTFVELAANQK" }, "paralog": false, "identity": 0.9911894273127754, "score": 877.0, "evalue": 0.0, "description": "Internal stop codon at 578732, 578777, 578798. Nonsense mutation around 578732, 578777, 578798.", "psc": { "uniref90_id": "UniRef90_D1J8L9", "gene": "PepB", "product": "Aminopeptidase (Leucine aminopeptidase)", "uniref50_id": "UniRef50_A0A448ZXA3", "cog_id": "COG0260", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8L9", "UniRef:UniRef50_A0A448ZXA3", "COG:COG0260", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXA3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXA3" ], "product": "Cytosol aminopeptidase" } }, "id": "GLJLFKCOHC_1002", "locus": "GCA013368035_01001" }, { "type": "cds", "sequence": "contig_1", "start": 580018, "stop": 580308, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "RefSeq:WP_175949952.1", "SO:0001217", "UniParc:UPI00158D643D", "UniRef:UniRef100_UPI00158D643D", "UniRef:UniRef50_A0A454CA46", "UniRef:UniRef90_A0A454CA46" ], "nt": "ATGAAGAAGAGTAAAAAAATAATTTTGTCTATTTTAGGAGCAAGTTCTTTAATTTCATTACCAATAGTAGCAATATCGTGTTCAAAATTGACATCAAAGCAACAACAAGTTAAATCAAAAATAAAGCAATTACTTGATATGGCAAAAAAGATACCATCTGCCGGTGAAATTATGAACCAATATGGAAAAATGATAAAAGATGAAGAAATTGCAAAACTAAATGATCAACAATGCGACGCAATCTTATTTAGCATAGATTTAGTTATTAATCAACTTAAAACTATTAAATAA", "aa": "MKKSKKIILSILGASSLISLPIVAISCSKLTSKQQQVKSKIKQLLDMAKKIPSAGEIMNQYGKMIKDEEIAKLNDQQCDAILFSIDLVINQLKTIK", "aa_hexdigest": "78506a13b0b52687030331d12cbe541a", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI00158D643D", "ncbi_nrp_id": "WP_175949952.1", "uniref100_id": "UniRef100_UPI00158D643D", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158D643D", "RefSeq:WP_175949952.1", "UniRef:UniRef100_UPI00158D643D" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158D643D", "uniref90_id": "UniRef90_A0A454CA46" }, "psc": { "uniref90_id": "UniRef90_A0A454CA46", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A454CA46" }, "pscc": { "uniref50_id": "UniRef50_A0A454CA46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA46" ], "product": "Variable surface lipoprotein" }, "genes": [], "id": "GLJLFKCOHC_1003", "locus": "GCA013368035_01002" }, { "type": "cds", "sequence": "contig_1", "start": 580386, "stop": 580517, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTAAACAAATAATAGTTTATGCGCCACATCCTTCACCTCTTGCTGCATATAAAGGATTTTTTGGCTCAAATGTTTTTAAAAAAATAAATTTTAAACTTAAATCATTATCAAAAGATGAAATCGATTGA", "aa": "MSKQIIVYAPHPSPLAAYKGFFGSNVFKKINFKLKSLSKDEID", "aa_hexdigest": "6572a11bb18712a3dec1c80bc831498f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4858.655100000001, "isoelectric_point": 9.6994779586792 }, "id": "GLJLFKCOHC_1004", "locus": "GCA013368035_01003" }, { "type": "cds", "sequence": "contig_1", "start": 580704, "stop": 581021, "strand": "-", "frame": 3, "gene": "ung", "product": "uracil-DNA glycosylase", "db_xrefs": [ "COG:COG0692", "COG:L", "EC:3.2.2.27", "GO:0004844", "GO:0006284", "KEGG:K03648", "SO:0001217", "UniRef:UniRef50_D1J8M1", "UniRef:UniRef90_D1J8M1" ], "nt": "ATGTCAAAATTTGCTTTTTTAGAATTTTTAAAAAAAGAAACTTCAAAACCATATTTTGAAGTCTTAAAAAAATTAACTATGTTGCCTAATGTAACGCCAAAGCCAAACAGAGTATTTTTTGCATTTAAAAATTTCGATTTTGACAACCTAAAGGTTATTATTATTGGCCAAGATCCTTATCCCAACAAAGGTGACGCAGATGGTATGTGTTTTTCAAGCAATGCAACAAAATGCCCGGCATCTTTAAAAAATATATTTATTGAAATAAAAAATGAATATCCAAATTCAACATTTGAAACTTTTTCGCTAAATTATTGA", "aa": "MSKFAFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNY", "aa_hexdigest": "6462a89db03abe20018c647a81e71307", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "ung" ], "truncated": "5-prime", "pseudogene": { "start": 580401, "stop": 581021, "observations": { "mutation": [ 580625, 580706 ], "stop": [ 580625, 580706 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M1", "query_cov": 1.0, "subject_cov": 0.4861111111111111, "identity": 0.99, "score": 215.0, "evalue": 2.38e-69, "gene_start": 1, "gene_end": 105, "reference_start": 1, "reference_end": 105, "reference_sequence": "MSKFVFLEFLKKETSKPYFEVLKKLTMLPNVTPKPNRVFFAFKNFDFDNLKVIIIGQDPYPNKGDADGMCFSSNATKCPASLKNIFIEIKNEYPNSTFETFSLNYWKNQGVLLLNSILTNIEGKTLAHKNLGWELFNQNLLTEINSCYSNIIYLILGNNAKDFIKNIDMSKQIIVYAPHPSPLAAYKGFFGSDVFKKINFKLKSLSKDGIDWSTKK" }, "paralog": false, "identity": 0.9757281553398058, "score": 405.0, "evalue": 8e-147, "description": "Internal stop codon at 580625, 580706. Nonsense mutation around 580625, 580706.", "psc": { "uniref90_id": "UniRef90_D1J8M1", "gene": "Ung", "product": "uracil-DNA glycosylase", "ec_ids": [ "3.2.2.27" ], "uniref50_id": "UniRef50_D1J8M1", "cog_id": "COG0692", "cog_category": "L", "kegg_orthology_id": "K03648", "go_ids": [ "GO:0004844", "GO:0006284" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M1", "EC:3.2.2.27", "UniRef:UniRef50_D1J8M1", "COG:COG0692", "COG:L", "KEGG:K03648", "GO:0004844", "GO:0006284" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M1" ], "product": "Uracil-DNA glycosylase" } }, "id": "GLJLFKCOHC_1005", "locus": "GCA013368035_01004" }, { "type": "cds", "sequence": "contig_1", "start": 581024, "stop": 581329, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02911.23" ], "nt": "TTGTTTGAAATTTACAACGGCCAATATAATCGAGTGGCTCAGGATGAATCTAAAGTAACATTTGCTCCTAAGATATTAAATGGTATGGAAAGGATTGATCAAAGCGAAAATCTTTCAAGTGTTGTAAACAAAATCAGAGCTTTTAATGATGAACCAGGTGCATATTTAATTTTTCAAAATAAAAGATTAAAAATCTTCCGGGCTTCAACAACAAAAATTAGTACCCCATTGAAAATAAAATTTAATGATGGATATTTGTACTTAATAGAATATCAATTTGAAGGGAAAAAAAGAGTAAGAGTTTAA", "aa": "MFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIFQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV", "aa_hexdigest": "e6c751612b4fecbc4246db4a139adee5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02911.23", "name": "Formyl_trans_C", "length": 86, "aa_cov": 0.7128712871287128, "hmm_cov": 1.0, "evalue": 9.471892480527858e-13, "score": 43.66648864746094, "start": 28, "stop": 99 } ], "seq_stats": { "molecular_weight": 11846.478999999987, "isoelectric_point": 9.653898811340333 }, "id": "GLJLFKCOHC_1006", "locus": "GCA013368035_01005" }, { "type": "cds", "sequence": "contig_1", "start": 581330, "stop": 581941, "strand": "-", "frame": 1, "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "db_xrefs": [ "COG:COG0223", "COG:J", "EC:2.1.2.9", "GO:0004479", "GO:0005737", "GO:0071951", "SO:0001217", "UniRef:UniRef50_B3PNE5", "UniRef:UniRef90_D1J8M2" ], "nt": "TTGCTTGAAATACAAGATAATAAGTTTATAAAAAATAATTTATTTTATAATTTTGATATGAAAAATATAAATAACAAAATTAATTTAGTTCTAGCGGGAACTGGACAATTTTCTGCTAAAACTTTTGAACCTCTTTTAAATGATGAAAGGTTCAATGTTTTGGCATTTATTTCTCAGCCAAATAGAAAATTGGATCGAAACAAAAATGTAATTGAAACAGAAATTTTTAAACTTGCCAAACAGTATGATATAAAAGTTTTTCAACCTCAAAAAATAAAAGAAATTTTGCCCGAACTTAGCGCAATGGATTTTGACTTTTTTATCACAGCTTCATTTGGACAATTTATACCTGATTCAATATTAAAATTACCTAAAAAAATGCCCTTGAACGTTCACGGAAGTTTGCTTGAAAAATATCGTGGAGCTGCGCCTGTGCAATATGCATTACTCAATGGTGATATTGAAACAGGAATAACATTAATTGAAATGGTAAAACAAATGGATGCAGGAGATATCCTAGAATCCTCGAAAGTGAAAATTGATTCTACAGATACAGCAATTGAAGTATTTGATAAATTAGCAAATGCGGCCTCGCAAAATCTTGGTAATTGA", "aa": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILESSKVKIDSTDTAIEVFDKLANAASQNLGN", "aa_hexdigest": "c21f718161a4d8369727ba02f64e17ab", "start_type": "TTG", "rbs_motif": null, "genes": [ "fmt" ], "truncated": "5-prime", "pseudogene": { "start": 581027, "stop": 581941, "observations": { "mutation": [ 581332 ], "stop": [ 581332 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M2", "query_cov": 1.0, "subject_cov": 0.6655737704918033, "identity": 0.975, "score": 384.0, "evalue": 2.91e-133, "gene_start": 1, "gene_end": 203, "reference_start": 1, "reference_end": 203, "reference_sequence": "MLEIQDNKFIKNNLFYNFDMKNINNKINLVLAGTGQFSAKTFEPLLNDERFNVLAFISQPNRKLDRNKNVIETEIFKLAKQYDIKVFQPQKIKEILPELSAMDFDFFITASFGQFIPDSILKLPKKMPLNVHGSLLEKYRGAAPVQYALLNGDIETGITLIEMVKQMDAGDILKSSKVKIDSIDTAIEVFDKLADVASKNLGNWLFEIYNGQYNRVAQDESKVTFAPKILNGMERIDQSENLSSVVNKIRAFNDEPGAYLIYQNKRLKIFRASTTKISTPLKIKFNDGYLYLIEYQFEGKKRVRV" }, "paralog": false, "identity": 0.9736842105263158, "score": 582.0, "evalue": 1.17e-213, "description": "Internal stop codon at 581332. Nonsense mutation around 581332.", "psc": { "uniref90_id": "UniRef90_D1J8M2", "gene": "fmt", "product": "methionyl-tRNA formyltransferase", "ec_ids": [ "2.1.2.9" ], "uniref50_id": "UniRef50_B3PNE5", "cog_id": "COG0223", "cog_category": "J", "go_ids": [ "GO:0004479", "GO:0005737", "GO:0071951" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M2", "EC:2.1.2.9", "UniRef:UniRef50_B3PNE5", "COG:COG0223", "COG:J", "GO:0004479", "GO:0005737", "GO:0071951" ] }, "pscc": { "uniref50_id": "UniRef50_B3PNE5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PNE5" ], "product": "Methionyl-tRNA formyltransferase" } }, "id": "GLJLFKCOHC_1007", "locus": "GCA013368035_01006" }, { "type": "cds", "sequence": "contig_1", "start": 581982, "stop": 582380, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAGAAGCAAATAGCAAAGATCTAATTGCAAGGTTAACAATTTTGAAATTCTTGAATCAACAAAAAGATTTATTTCTAGCAAATAATAATGCATTGCCAGCCAATACACCAATTGGAATAAAATATAGTCAATATAATATATTTAATATTAACAACGAATATAAAACACTAAATATTTTAAATGTTTATCAACCTATAATTTTAAAATACTCAACCATTGCTGAAACATTCAATCAAAATGGTTTGGACCAATTGTTAAAGAATAATGAATTTGCAAACTATTTTAAATTTTTAGACAGTAGTTACATTCAATTTTCAAATAACGTTTTAAGCAATGATAGTTTAACAAAAATAGGTTCAAAAACAGAAAATTTATTGAACTTCATATTGCCTTGA", "aa": "MKEANSKDLIARLTILKFLNQQKDLFLANNNALPANTPIGIKYSQYNIFNINNEYKTLNILNVYQPIILKYSTIAETFNQNGLDQLLKNNEFANYFKFLDSSYIQFSNNVLSNDSLTKIGSKTENLLNFILP", "aa_hexdigest": "d7c40f680cf749252d7b96cc8861e49e", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15178.125699999986, "isoelectric_point": 8.776935386657716 }, "id": "GLJLFKCOHC_1008", "locus": "GCA013368035_01007" }, { "type": "cds", "sequence": "contig_1", "start": 582960, "stop": 583148, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTGGGAAGTACAATAACCATTGGGCCGGTGGCAATAGCCTGCATTTCACTGCCAACAGCAATATTTGAGTTTAAAAAATCAACAATTTTAAAAAGAATTGGCGCCTCTAGCATTAAGCCTAAATCGTTTTTGGCATATACAGCAATATTTTATTTAATTATAATGATTTGTTCTGGCCTTTGA", "aa": "MILGSTITIGPVAIACISLPTAIFEFKKSTILKRIGASSIKPKSFLAYTAIFYLIIMICSGL", "aa_hexdigest": "acd0c1187f8c0e5ab3adecb3c36f4f7d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6638.100099999999, "isoelectric_point": 9.69825305938721 }, "id": "GLJLFKCOHC_1009", "locus": "GCA013368035_01008" }, { "type": "cds", "sequence": "contig_1", "start": 583330, "stop": 584175, "strand": "-", "frame": 2, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_052521867.1", "SO:0001217", "UniParc:UPI0006964018", "UniRef:UniRef100_UPI0006964018", "UniRef:UniRef50_D1J8M4", "UniRef:UniRef90_D1J8M4" ], "nt": "GTGGAAAATGAAAAAGTTTGTAAAAATAAAAGTCTAGAAATAATAGACAATTTTTTAAGTAATTTAGAAGATCATGAAAAAAACACAATTAAAGCAAATTCAATTGAAATGCGCCAATACAATTTAAAAAAGTTGGTGAAAATTAAAAATTTGACAAAAGAATTCAAAATAAAAAAAGAAAAAATAAAAGTTGTAGATAATTTGAATCTCGAAATTTATAAGAATCAAAACATTGCTCTATTAGGTGGAAATGGAGCCGGAAAAACTACAACAGTTGAAATGCTTATAGGAATTTTAAAACCTACCTCAGGCAATATTGAATATTATTTTAATGATGGATCAAATCATAATGAAAGTGCACAAACAAACATAGGAATTCAATTTCAGGATTCTTCATATCCTCAAGGTTTATCGGTTAATGATGTCATCTATTCAATGAACAAAATCTATGGCAATAAAAGTTCACCAGAAGAATTGCAAAAACTTATAAAGATTTTTGGGGTTGATGAATTTATAAATAATAAAGCTTCTTCCTTAAGCGGGGGCCAACATCAAAGACTTAATGCATTGCTTGCAATAATTAATAAACCAAAGTTGTTAATATTAGATGAATTAAGCACTGGTCTTGACATAAAAATAAAAACAAGATTGGTATCATTTTTGAATGACTATATCAAAGAAATTGATTGTACTTTGATTTTAATATCTCACGATATAAATGAAATTGAAATTCTGGCAGATAGAATTATTATTATGAATAAAGGTAGAATTGTCCTTGATGCTTTGAAAAGCGAAATAATTGAAAAATATGGATCAATATCTCAATGTCTTAAGGAATATATTTAA", "aa": "MENEKVCKNKSLEIIDNFLSNLEDHEKNTIKANSIEMRQYNLKKLVKIKNLTKEFKIKKEKIKVVDNLNLEIYKNQNIALLGGNGAGKTTTVEMLIGILKPTSGNIEYYFNDGSNHNESAQTNIGIQFQDSSYPQGLSVNDVIYSMNKIYGNKSSPEELQKLIKIFGVDEFINNKASSLSGGQHQRLNALLAIINKPKLLILDELSTGLDIKIKTRLVSFLNDYIKEIDCTLILISHDINEIEILADRIIIMNKGRIVLDALKSEIIEKYGSISQCLKEYI", "aa_hexdigest": "e0f3e08e7ad024974e8fba8eed628731", "start_type": "GTG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0006964018", "ncbi_nrp_id": "WP_052521867.1", "uniref100_id": "UniRef100_UPI0006964018", "db_xrefs": [ "SO:0001217", "UniParc:UPI0006964018", "RefSeq:WP_052521867.1", "UniRef:UniRef100_UPI0006964018" ] }, "ips": { "uniref100_id": "UniRef100_UPI0006964018", "uniref90_id": "UniRef90_D1J8M4" }, "psc": { "uniref90_id": "UniRef90_D1J8M4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_D1J8M4", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_D1J8M4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M4" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "GLJLFKCOHC_1010", "locus": "GCA013368035_01009" }, { "type": "cds", "sequence": "contig_1", "start": 584282, "stop": 584884, "strand": "-", "frame": 1, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "db_xrefs": [ "COG:COG0344", "COG:I", "EC:2.3.1.15", "EC:2.3.1.275", "GO:0005886", "GO:0006654", "GO:0008374", "KEGG:K08591", "SO:0001217", "UniRef:UniRef50_D1J8M5", "UniRef:UniRef90_D1J8M5" ], "nt": "TTGGCAATAATAATAACTAAATTAAGAGGGAATGATATAAGAAAAATTGGCTCGCAAAATCCTGGAGCAACAAACGCATTAAGAAATTTTGGAATAAAATTTGCAGCATTAGTTTTTGCTTTTGACATTTTTAAAGCCTTTTTAGCAACAATAATTGCATTTTTAATTAAAAAATATCTAAGAAATCTTGAATATATTTTTCCAATGCTAGCAGGGCTTGGTGCAGTAATTGGACACGTCTTTCCAATTTATTTTAAATTTAAAGGCGGCAAAGGAGTAGCTTGCTTTTTTGGAATAATGCTTGCATATAATTTTCTATTATTTTTAATTTTTGTAGCAATATATGTAATACTAGTTTTTTCAACAAAATATGTTTCACTTGCTTCATCAATAAGCTGCGGAATAATTCCTTATTTAGGTTTAATTTATATATTTTATGCAGGAACAATATTTGGTTATATGCAACAAAATGCTATATATCCCGCACATGTAATTATTTTAATATTGGCTTCGCTAATAATAGTTGTTAAACATATACCCAATTTTAAACGAATATTTAAACATACAGAACCAAAAATTTTTAGCAAAACAAAAAAAGCATAA", "aa": "MAIIITKLRGNDIRKIGSQNPGATNALRNFGIKFAALVFAFDIFKAFLATIIAFLIKKYLRNLEYIFPMLAGLGAVIGHVFPIYFKFKGGKGVACFFGIMLAYNFLLFLIFVAIYVILVFSTKYVSLASSISCGIIPYLGLIYIFYAGTIFGYMQQNAIYPAHVIILILASLIIVVKHIPNFKRIFKHTEPKIFSKTKKA", "aa_hexdigest": "247252e5ff46395546bc9564bb3e0257", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8M5", "query_cov": 0.995, "subject_cov": 0.868995633187773, "identity": 0.985, "score": 368.0, "evalue": 6.31e-128, "valid": true, "gene": "plsY", "product": "glycerol-3-phosphate 1-O-acyltransferase PlsY", "ec_ids": [ "2.3.1.15", "2.3.1.275" ], "uniref50_id": "UniRef50_D1J8M5", "cog_id": "COG0344", "cog_category": "I", "kegg_orthology_id": "K08591", "go_ids": [ "GO:0005886", "GO:0006654", "GO:0008374" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M5" ], "product": "Glycerol-3-phosphate acyltransferase" }, "genes": [ "plsY" ], "id": "GLJLFKCOHC_1011", "locus": "GCA013368035_01010" }, { "type": "cds", "sequence": "contig_1", "start": 585032, "stop": 585409, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "RefSeq:WP_036438733.1", "SO:0001217", "UniParc:UPI000513208E", "UniRef:UniRef100_A0A454CA59", "UniRef:UniRef50_D1J8M6", "UniRef:UniRef90_D1J8M6" ], "nt": "ATGGAATTATTAAAATATAGTTTAATTACAAAAAACGAATTCAATAATGAACCCTCTAAAACGATAGAAACAGAATTTGTTCCATATACCGAAGAAGTTGTTGATGAATTTATTCATATAAATTTTATTGATAATAATAATCAGGAATGTGATTTATTGGTCAATAGTGATACTTTAAAAATAAAATATTCTGGTTCATATATTGAGTTACAAAAAAACAAAAGAGTAAGCAATAAAATTCAAGAATTTTCTCTAGATTTTTATTTAAAGGATGTTTTTATTGAAGGAAACAAGATATCTGCTCATTATGATATTTTAGTTGGTAGTGATGTTGTTGCAAGCAATATAATTAACATTGAAACAAAGACCGAAAAATAG", "aa": "MELLKYSLITKNEFNNEPSKTIETEFVPYTEEVVDEFIHINFIDNNNQECDLLVNSDTLKIKYSGSYIELQKNKRVSNKIQEFSLDFYLKDVFIEGNKISAHYDILVGSDVVASNIINIETKTEK", "aa_hexdigest": "df684aec66dd184348c9c9a0a8c296fb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000513208E", "ncbi_nrp_id": "WP_036438733.1", "uniref100_id": "UniRef100_A0A454CA59", "db_xrefs": [ "SO:0001217", "UniParc:UPI000513208E", "RefSeq:WP_036438733.1", "UniRef:UniRef100_A0A454CA59" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CA59", "uniref90_id": "UniRef90_D1J8M6" }, "psc": { "uniref90_id": "UniRef90_D1J8M6", "uniref50_id": "UniRef50_D1J8M6" }, "pscc": { "uniref50_id": "UniRef50_D1J8M6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14520.160299999989, "isoelectric_point": 4.650191307067872 }, "id": "GLJLFKCOHC_1012", "locus": "GCA013368035_01011" }, { "type": "cds", "sequence": "contig_1", "start": 585390, "stop": 585734, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF19269.4" ], "nt": "TTGTTTGTTGAAACTTATAAACAATCAAGTCCAACGATTTCAACATTAAAGAAAGATTTAGAAATTTATTTAAACCCAAAAGAAAATTGCGACTTAGAGTTAGAAAGATTAGATGTTGTAAAAGTTTTTTATAAAGAGTTAATTTCAAGAGAATTCAATATAGAAAATATACAAAAAGCAATTGATGAAACAAAAACTATCTTGAATGTTAAGGGAAGAGATTTGTTCATGCCAATTAGAAAAGCAACAACATTTGAAGAACATGGCCCTGAACTTGCAAAGGCAATCTATCTTTTTGGGGCTGAATTAGTTTTTAAGAGATTAAATGGAATTATTAAAATATAG", "aa": "MFVETYKQSSPTISTLKKDLEIYLNPKENCDLELERLDVVKVFYKELISREFNIENIQKAIDETKTILNVKGRDLFMPIRKATTFEEHGPELAKAIYLFGAELVFKRLNGIIKI", "aa_hexdigest": "7a32e9ff1bfeb7e9472424c9302cc23c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF19269.4", "name": "Anticodon_2", "length": 80, "aa_cov": 0.6929824561403509, "hmm_cov": 1.0, "evalue": 7.812916241008243e-11, "score": 37.96106719970703, "start": 33, "stop": 111 } ], "seq_stats": { "molecular_weight": 13297.378299999978, "isoelectric_point": 6.60391788482666 }, "id": "GLJLFKCOHC_1013", "locus": "GCA013368035_01012" }, { "type": "cds", "sequence": "contig_1", "start": 585930, "stop": 586046, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F", "UniRef:UniRef90_UPI003594285F" ], "nt": "ATGGAAGGAAAAAAGCTTTCAAAAAGAGATAAAACTTTAAAACAATTTATTGAAGACTATAAAAATGAAGGCTATCCTGCAGCAGCAATAATGAATTTTTTAGCCTTGTTAGGTTGA", "aa": "MEGKKLSKRDKTLKQFIEDYKNEGYPAAAIMNFLALLG", "aa_hexdigest": "57ad0b88b87cbb936a0cd5b0b7ea7e03", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 585882, "stop": 586139, "observations": { "mutation": [ 585932, 586088 ], "stop": [ 585932, 586088 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI003594285F", "query_cov": 1.0, "subject_cov": 0.4470588235294118, "identity": 0.8420000000000001, "score": 63.2, "evalue": 3.54e-12, "gene_start": 1, "gene_end": 38, "reference_start": 32, "reference_end": 69, "reference_sequence": "EHITNTPKQLAIYDAFGWETPRFGHLTIITNMEGKKLSKRDKFLKQFIEDYKNEGYRPDAIFNFLVLLGWTSADKSEVMSHQEII" }, "paralog": false, "identity": 0.8, "score": 136.0, "evalue": 1.28e-43, "description": "Internal stop codon at 585932, 586088. Nonsense mutation around 585932, 586088.", "psc": { "uniref90_id": "UniRef90_UPI003594285F", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI003594285F", "UniRef:UniRef50_UPI003594285F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI003594285F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI003594285F" ], "product": "glutamate--tRNA ligase family protein" } }, "id": "GLJLFKCOHC_1014", "locus": "GCA013368035_01013" }, { "type": "cds", "sequence": "contig_1", "start": 586086, "stop": 586379, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001A2605D2" ], "nt": "TTGCAAATTTCTAATGAAGAAAAGCAAAGAAGAGAAGAAGCTAAAGAATATTCAATAAGATTAAAATTGCCAGCAAATAAAGATTATGCTTGGAATGACTTAGTAAGAGGCAAAATCTCAGTTAATAGTGATGATATAGGAGATTTTGTTATTATGAAAAGTGATGGTTATCCAACATATAATTTTGCAGTTGTTGTTGATGACCACCAAATGGAAATTTCACATGTACTAAGAGGCGAAGAACACATCACAAACACACCAAAGCAATTAGCAATATACGATGCATTCGGATGA", "aa": "MQISNEEKQRREEAKEYSIRLKLPANKDYAWNDLVRGKISVNSDDIGDFVIMKSDGYPTYNFAVVVDDHQMEISHVLRGEEHITNTPKQLAIYDAFG", "aa_hexdigest": "aff7873dc2ec53cc08c3191a1c34f16c", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00056C184B", "query_cov": 0.8350515463917526, "subject_cov": 0.8181818181818182, "identity": 0.617, "score": 114.0, "evalue": 4.76e-31, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_UPI001A2605D2" }, "pscc": { "uniref50_id": "UniRef50_UPI001A2605D2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001A2605D2" ], "product": "glutamate--tRNA ligase family protein" }, "genes": [], "id": "GLJLFKCOHC_1015", "locus": "GCA013368035_01014" }, { "type": "cds", "sequence": "contig_1", "start": 586596, "stop": 586787, "strand": "-", "frame": 3, "gene": null, "product": "glutamate--tRNA ligase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7", "UniRef:UniRef90_UPI002208A1AD" ], "nt": "ATGAAAACTATAAGAACAAGATATGCACCGAGCCCAACTGGGTATTTGCACATTGGTGGTGCAAGAACTGCGTTATTTAACTATTTATTTGCAAAACATTTCAATGGTACTTTTGTTTTTAGGCTAGAAGATACTGACGTTGCTAGAAACGTTGTCGGAGGTGAAGAAAGCCAATTAAATAATTTAGCGTGA", "aa": "MKTIRTRYAPSPTGYLHIGGARTALFNYLFAKHFNGTFVFRLEDTDVARNVVGGEESQLNNLA", "aa_hexdigest": "025c80f5c6cbd3489fb6fa238db8d3c4", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0016AA1FE4", "query_cov": 0.9841269841269841, "subject_cov": 0.8611111111111112, "identity": 0.613, "score": 81.6, "evalue": 3.58e-19, "valid": false, "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A5J4P5N7" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 586422, "stop": 586778, "observations": { "mutation": [ 586598 ], "stop": [ 586598 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI002208A1AD", "query_cov": 0.9523809523809523, "subject_cov": 0.4444444444444444, "identity": 0.9, "score": 114.0, "evalue": 2.38e-31, "gene_start": 4, "gene_end": 63, "reference_start": 3, "reference_end": 62, "reference_sequence": "MKIRTRYAPSPTGYLHIGGARTALFCYLYAKHFNGQFIFRLEDTDVKRNVPGGEESQLNNLAWLGIIPDESPLNPNPIYGKYRQSEKLDRYWKIANELIEKGFAYKAYDNSDELEQQKKKVMKKEYLVLGMINID" }, "paralog": false, "identity": 0.7711864406779662, "score": 184.0, "evalue": 2.47e-61, "description": "Internal stop codon at 586598. Nonsense mutation around 586598.", "psc": { "uniref90_id": "UniRef90_UPI002208A1AD", "product": "glutamate--tRNA ligase family protein", "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI002208A1AD", "UniRef:UniRef50_A0A654IDE7" ] }, "pscc": { "uniref50_id": "UniRef50_A0A654IDE7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A654IDE7" ], "product": "Glutamate--tRNA ligase" } }, "id": "GLJLFKCOHC_1016", "locus": "GCA013368035_01015" }, { "type": "cds", "sequence": "contig_1", "start": 587110, "stop": 587652, "strand": "-", "frame": 2, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8", "UniRef:UniRef90_D1J8M8" ], "nt": "ATGAAAAAACAAGCAATTATGACAAAATTTTTACTTTGTATAGGAAGTGTTGCTCCTGTGTTTGCATTGCCACTAGTTGCGGCAAGCTGTAATAAAACAGCAACCATAACTCTCAATTCAAAAGCGAAAGAATCATTTGAAAAATATGGTTATCACGTTAAGGGTTCTGCACTTGATTTTAATAAAAATAATTATTCAGTAAGTAACCCGATATCGCCAAGAGATTCCTTGTACAAATTAACAAAAAATTTAAAAGATGAAAAAGGAAACCCAATTTACTTTAGAGATGCCAACGGAAATTTATTAAAATTTAAGGGTAAAAAATTGGAAAATGGTGAAATAGAATTCGATAGAGATGCTAATGGAAACTACATTTATGACAAAGAAAATGGATACCCAAGATTGAAAGAAGTATTGAATAATGAACTCGAAGTAATTGACGGAAAACCAGTGCAAAGAGTTTATAGAGAATTTTTTAGCAGATTTTTAGATTTTAATAATATTTCTCCAAATTATGATTTTAGAGTTTTCTCTTTTACATGA", "aa": "MKKQAIMTKFLLCIGSVAPVFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDANGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFT", "aa_hexdigest": "b734f13cf62418a6f3f6c36a473dbf86", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 586807, "stop": 587652, "observations": { "mutation": [ 586848, 586893, 586983, 587016, 587061, 587112 ], "stop": [ 586848, 586893, 586983, 587016, 587061, 587112 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8M8", "query_cov": 1.0, "subject_cov": 0.6360424028268551, "identity": 0.9940000000000001, "score": 340.0, "evalue": 1.71e-116, "gene_start": 1, "gene_end": 180, "reference_start": 1, "reference_end": 180, "reference_sequence": "MKKQAIMTKFLLCIGSVAPMFALPLVAASCNKTATITLNSKAKESFEKYGYHVKGSALDFNKNNYSVSNPISPRDSLYKLTKNLKDEKGNPIYFRDANGNLLKFKGKKLENGEIEFDRDANGNYIYDKENGYPRLKEVLNNELEVIDGKPVQRVYREFFSRFLDFNNISPNYDFRVFSFTWEELINYFPATKNDSRYWPYRDNPRALFLVLYWTLKQNEAAPNWKSDFLDPSIESLLAKGKNRNVNTSIEEAPWPFYKGIMKGNKDAFWSNASEPITAIFEKP" }, "paralog": false, "identity": 0.9750889679715302, "score": 526.0, "evalue": 1.81e-192, "description": "Internal stop codon at 586848, 586893, 586983, 587016, 587061, 587112. Nonsense mutation around 586848, 586893, 586983, 587016, 587061, 587112.", "psc": { "uniref90_id": "UniRef90_D1J8M8", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8M8", "UniRef:UniRef50_D1J8M8" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8M8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8M8" ], "product": "Variable surface lipoprotein" } }, "id": "GLJLFKCOHC_1017", "locus": "GCA013368035_01016" }, { "type": "cds", "sequence": "contig_1", "start": 587819, "stop": 588322, "strand": "+", "frame": 2, "gene": null, "product": "cysteine peptidase family C39 domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2", "UniRef:UniRef90_UPI001E465ED2" ], "nt": "ATGATTTGCCACTTTTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAATACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAGAAAATTTCTGATTTTGTGGGGTTTGATACTAATATTTACAAAGTAGATTTTTTAAGTTTTATTTCAAATTTGAATGATGTTTCATTTCCGTTTGTCACAATATTGAAAAATAATAATTTTTTGCATTATACAATTGTTAAAAGAATTGATAAAGCCTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAAATAAATTCATAAATGTTGTAGTTATATTAAGCAAAAAATTTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAGCACAAAACACAAAGTTAACAATAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTATATTAAATATGTTTTAAGCGTTTTATGA", "aa": "MICHFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDVSFPFVTILKNNNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKNKFINVVVILSKKFKTPNCTKINYNLYLAQNTKLTIISIFLAILFNALMLSSSFYIKYVLSVL", "aa_hexdigest": "a5581690d918a2a561de81bbd18be779", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 587762, "stop": 588602, "observations": { "insertion": [ 588584 ], "mutation": [ 587765, 588320, 588371, 588398 ], "stop": [ 587765, 588320, 588371, 588398 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E465ED2", "query_cov": 1.0, "subject_cov": 0.5985663082437276, "identity": 0.982, "score": 317.0, "evalue": 1.68e-107, "gene_start": 1, "gene_end": 167, "reference_start": 20, "reference_end": 186, "reference_sequence": "MWINKQDSVKDCAIYTLQAMICHFFKYKADINFLKKNTIYDEEGVSLYSFKKISDFVGFDTNIYKVDFLSFISNLNDVSFPFVTILKNDNFLHYTIVKRIDKAFVYLCDSEFLNYRLEINEFKDKFINVVVILSKKFKTPNCTKINYNLYLTQNTKLTIISIFLAILFNALMLSSSFYIKYVLSVLWSENANNRLLSILIIFSWIAILKAITWLFKKIIINKLTFYYEKKLYLLVKERLKFASQKDLQKINQNDFMARILSISTITEYKAGLFLFYLKI" }, "paralog": false, "identity": 0.9713261648745519, "score": 518.0, "evalue": 2.11e-189, "description": "Internal stop codon at 587765, 588320, 588371, 588398. Frameshift due to insertion around 588584. Nonsense mutation around 587765, 588320, 588371, 588398.", "psc": { "uniref90_id": "UniRef90_UPI001E465ED2", "product": "cysteine peptidase family C39 domain-containing protein", "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E465ED2", "UniRef:UniRef50_UPI001E465ED2" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E465ED2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E465ED2" ], "product": "cysteine peptidase family C39 domain-containing protein" } }, "id": "GLJLFKCOHC_1018", "locus": "GCA013368035_01017" }, { "type": "cds", "sequence": "contig_1", "start": 588458, "stop": 588913, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAAAAAGACCTGCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTTCAACTATTGCAGAATATAAGGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACATTGCTTTTGGGTCTGTTTAACTATAGAATATTATTAATTTGCTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATATTCAATAATTATTTTTTAAATTATGAATATAGGACGAATGATTTAAATAATTCGAATTCTAAATTATTTCAATCGTTATTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGA", "aa": "MLVKERLKFASQKDLQKINQNDFMARILSISTIAEYKAGLFLFLFENIIIFISSTLLLGLFNYRILLICLLGISITFAISLFSLKYQSKKATELINLKIKSIKIFNNYFLNYEYRTNDLNNSNSKLFQSLFNTQYNLGIFNETKDSFITFI", "aa_hexdigest": "0a630f70bc6b5430a08a7be35618cb39", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17647.52529999998, "isoelectric_point": 9.546881294250486 }, "id": "GLJLFKCOHC_1019", "locus": "GCA013368035_01018" }, { "type": "cds", "sequence": "contig_1", "start": 589016, "stop": 589306, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCCCCTATTGTAAATTTAGTCTTCCTGATTTCAAATTCTTCAATAAATAAACAAGGAATAATAAGAATAAAATATTTATTAGAAATCACACAAAACAAGGATGGAAATATATCGTTCAAACCCCAGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAAAAATCTTAATTTAACAATTAAAAACAATCTAGAAATATCAGGGAAAAATGGAGTTGGTAAAAGTTTGATTTTGAATGCTATATTTAAAGTTTATTGA", "aa": "MNSPIVNLVFLISNSSINKQGIIRIKYLLEITQNKDGNISFKPQKQNKINILNASINIGSKNVIKNLNLTIKNNLEISGKNGVGKSLILNAIFKVY", "aa_hexdigest": "0f273c1a95af9b574aeff589db3ef117", "start_type": "TTG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10652.41649999999, "isoelectric_point": 10.253003501892088 }, "id": "GLJLFKCOHC_1020", "locus": "GCA013368035_01019" }, { "type": "cds", "sequence": "contig_1", "start": 589424, "stop": 589780, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATTGCATATGTAAGAAAGACAATATAAAGGAAAATATTTTTCTTAAAAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGAGGAAATAACCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAACATTACAAATGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCTATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTCGCTTTAAAAAAATAA", "aa": "MDCICKKDNIKENIFLKNFKKYNLNNLFLDMNLPLNKYIENGGNNLSSGQKEFIIIMRLFCQKYYFILLDEAFENITNEIFLKLKFLILNYQDRAIFIEISHNKKIVANKRKIFALKK", "aa_hexdigest": "ad3492312072d939909b2d012609c73b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14151.632599999977, "isoelectric_point": 9.444441032409667 }, "id": "GLJLFKCOHC_1021", "locus": "GCA013368035_01020" }, { "type": "cds", "sequence": "contig_1", "start": 589839, "stop": 590291, "strand": "+", "frame": 3, "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "db_xrefs": [ "COG:COG0319", "COG:J", "GO:0004540", "GO:0042254", "GO:0046872", "RefSeq:WP_012855716.1", "SO:0001217", "UniParc:UPI0001BC249E", "UniRef:UniRef100_D1J8N0", "UniRef:UniRef50_A0A448ZWQ4", "UniRef:UniRef90_D1J8N0" ], "nt": "ATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAATCAAAAATAGAATTTGGTGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCTAAAAAGATGAAAAAGTTAAATAACTTACACAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCATGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGGATTGCTCACCTATTTGGTTTTGACCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTCGATAATATAATGAATAAATTAAACATAAAGAGAATATAA", "aa": "MNRINFKNQSFHRFNFMKDFLDILEESKIEFGAKKSLCVDVMFVTAKKMKKLNNLHRQKNYTTDILSFPLDSQEELNFLDEIELGQIIISPWKIKKQAKEFNHSLRREFCYIFAHGIAHLFGFDHITEEEAKIMNGHVDNIMNKLNIKRI", "aa_hexdigest": "8a34892946feb09a64a863af62f051c6", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI0001BC249E", "ncbi_nrp_id": "WP_012855716.1", "uniref100_id": "UniRef100_D1J8N0", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC249E", "RefSeq:WP_012855716.1", "UniRef:UniRef100_D1J8N0" ] }, "ips": { "uniref100_id": "UniRef100_D1J8N0", "uniref90_id": "UniRef90_D1J8N0" }, "psc": { "uniref90_id": "UniRef90_D1J8N0", "gene": "ybeY", "product": "rRNA maturation RNase YbeY", "uniref50_id": "UniRef50_A0A448ZWQ4", "cog_id": "COG0319", "cog_category": "J", "go_ids": [ "GO:0004540", "GO:0042254", "GO:0046872" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZWQ4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZWQ4" ], "product": "Endoribonuclease YbeY" }, "genes": [ "ybeY" ], "id": "GLJLFKCOHC_1022", "locus": "GCA013368035_01021" }, { "type": "cds", "sequence": "contig_1", "start": 590305, "stop": 591150, "strand": "+", "frame": 1, "gene": "era", "product": "GTPase Era", "db_xrefs": [ "COG:COG1159", "COG:J", "GO:0003723", "GO:0005525", "SO:0001217", "UniRef:UniRef50_A0A501XB37", "UniRef:UniRef90_D1J8N1" ], "nt": "ATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGCTAAATTTTAACCTATCAATAATTACGGATAAGCCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTACCAAATCATTTTTATTGATACTCCAGGAATTCACAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAGCAATCGGAACAGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCGGTCATTTCAAAAATTGATTTATTAAATACAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAGGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTCAGATTTGATCAATGAAATTAAAACCTATTGCTATGAAGATGAACCTTTGTACCCTGATGATCAAATTGGGGATATAACATTAAGGTTTATGGCAAAAGAAATAATTAGAGAAATTGCTATAAACAATTTAAAATTTGAATTGCCACACTCAATTGCGGTTGAGATCGAACAATTTGACGATAAAAACGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATCGGAAAAGACGGCCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAGTTTTTGATCACAAAGTCTTTTTAAATATTAAGGTAAAAATTAGCGAAGATTGA", "aa": "MKKVCNVCLIGRPNVGKSTFLNNVLNFNLSIITDKPQTTKDNIRGIYNDKDYQIIFIDTPGIHKAENLLSERLNSKSYEAIENSDVVLFLTPANEAIGTGDKFIIEKINETNNENKIAVISKIDLLNTKEEADKKAEELKKLGFEKVFGIGQNLPTSYSDLINEIKTYCYEDEPLYPDDQIGDITLRFMAKEIIREIAINNLKFELPHSIAVEIEQFDDKNEDEDYKIYALIYVKKKSQKPILIGKDGQMIKKISIDSRHKMEKVFDHKVFLNIKVKISED", "aa_hexdigest": "f106e5c02bded14c057d8b201de985fb", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8N1", "query_cov": 1.0, "subject_cov": 0.95578231292517, "identity": 0.986, "score": 536.0, "evalue": 6.26e-192, "valid": true, "gene": "era", "product": "GTPase Era", "uniref50_id": "UniRef50_A0A501XB37", "cog_id": "COG1159", "cog_category": "J", "go_ids": [ "GO:0003723", "GO:0005525" ] }, "pscc": { "uniref50_id": "UniRef50_A0A501XB37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501XB37" ], "product": "GTPase Era" }, "genes": [ "era" ], "id": "GLJLFKCOHC_1023", "locus": "GCA013368035_01022" }, { "type": "cds", "sequence": "contig_1", "start": 591191, "stop": 591895, "strand": "+", "frame": 2, "gene": null, "product": "Hypothetical lipoprotein (MG045 family)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F", "UniRef:UniRef90_UPI00215D9E7F" ], "nt": "GTGAAACGATTAACTACACATAGAACGAGGGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTTTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAGAAAATAATTACCAAATTAAAACAAAATTATTCATATCACGCCTTTACAAATCTAGATGAGTTTACGAGAGCAATAAATAACGAAAAAGCCGTTGCCGGAGTTGGAAGTGATTACCAAATTGCTCAACTAATTCTTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTATTTAATGATTCAAAATTTAATGATTCAACCCAAATAAAGAAGCTTTATCCAGAAATTGTTAATAAACATTTTGATCTATTTGAAAAATTTATCATTAAAAAAATAAGACAAATAAATCCTAAAAACATAAAGCAAACTGATTGGCAAAATATTAGTCACTCCAAATTCTTGCCTTTTATTTATTATGACGAACATAACAATATTTTGGGTTTTGAAATTGACCAAAACCAAGGAGTTGACCACTTTTACGAATTTTTAATCCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAAGTCCAAATAAATTTACTGAAAGAAGCAATTTAAAAGATAATGAAAACTTTAACGATTTAGAAAGTTTAAATTCTTGA", "aa": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNDSKFNDSTQIKKLYPEIVNKHFDLFEKFIIKKIRQINPKNIKQTDWQNISHSKFLPFIYYDEHNNILGFEIDQNQGVDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKDNENFNDLESLNS", "aa_hexdigest": "f36ef2e22b51ebe1bcf58045488ed592", "start_type": "GTG", "rbs_motif": "AGGA", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 591191, "stop": 592198, "observations": { "mutation": [ 591893, 591947, 591956, 592040 ], "stop": [ 591893, 591947, 591956, 592040 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00215D9E7F", "query_cov": 1.0, "subject_cov": 0.612565445026178, "identity": 0.94, "score": 429.0, "evalue": 2.34e-149, "gene_start": 1, "gene_end": 234, "reference_start": 1, "reference_end": 234, "reference_sequence": "MKRLTTHRTRVIFIAIIALLTLGLFLIILFFKISSPFKVSIYNYESYLDKKIITKLKQNYSYHAFTNLDEFTRAINNEKAVAGVGSDYQIAQLILEKKLRKINFAKLFNNSKFNDSTQIKNLYPEIVNKHFDLFEKFIIKKIRQINPENFQQTDWQNISHTKFLPFIYYDKNNNILGFEIDQKKGIDHFYEFLIPYFIMDKMVVYNTDKSPNKFTERSNLKNNENFNNLESLSSWKDIIKTLVSKYKNPRVYWTNWFQDNAMIGQFYGYESLNKLKYFSKQNEWADINHENFKEIINDWVSLVKDATGESIKNSKTNKLATDGQELVSSIIEPINGKADVSIMYNGDALDAYYGEDNFKKLGNIPHIGFKRPKILIWILMLE" }, "paralog": false, "identity": 0.9432835820895522, "score": 632.0, "evalue": 7.7e-232, "description": "Internal stop codon at 591893, 591947, 591956, 592040. Nonsense mutation around 591893, 591947, 591956, 592040.", "psc": { "uniref90_id": "UniRef90_UPI00215D9E7F", "product": "Hypothetical lipoprotein (MG045 family)", "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00215D9E7F", "UniRef:UniRef50_UPI00215D9E7F" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00215D9E7F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215D9E7F" ] } }, "id": "GLJLFKCOHC_1024", "locus": "GCA013368035_01023" }, { "type": "cds", "sequence": "contig_1", "start": 592217, "stop": 592336, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAACGGAGATGCCTTAGATGCATATTATGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCGCATATTGGTTTTAAAAGACCAAAAAATACTTACATGAATATTGATGCTTGA", "aa": "MYNGDALDAYYGEDNFKKLGNIPHIGFKRPKNTYMNIDA", "aa_hexdigest": "274799ffbbd31f722b697248fbf20641", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4495.999700000002, "isoelectric_point": 6.514908027648925 }, "id": "GLJLFKCOHC_1025", "locus": "GCA013368035_01024" }, { "type": "cds", "sequence": "contig_1", "start": 592457, "stop": 592855, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGTTGAATCTAATATCAAAAGACAACAAATTAAAAAAAGATGAAATTGTTAATGAGCTATTTGCAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTGCAGAATATTTTGACTATTTCAGAGAAGCATTTGAAACAATGAATTTACCCGTTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTTTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTAGAGATATTCAATCCCGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAA", "aa": "MLNLISKDNKLKKDEIVNELFADKNMSMPKKIKDIKKDFFAEYFDYFREAFETMNLPVVNNFHSINYTPGFKNVKNFIEKWYFLNKNQEVDKTALEIFNPEFNKKINHRIYQPLDLNLKTKIIDYYFQKTKS", "aa_hexdigest": "7866475d9489341c5f9ac4ecfe57bca8", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 16023.323299999985, "isoelectric_point": 9.222347450256347 }, "id": "GLJLFKCOHC_1026", "locus": "GCA013368035_01025" }, { "type": "cds", "sequence": "contig_1", "start": 592883, "stop": 593080, "strand": "+", "frame": 2, "gene": null, "product": "ATP-binding cassette domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00112F2DA6" ], "nt": "ATGGAAAATAAAATTAAAACACCAAACAAAAATGAAGAACCAATAATCCAATTAGTAGACGTTGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTATTAGGTCCTTCGGGTTCAGGAAAAACAACCATATTGAGAATTATTGGTGGTTTTGAATGA", "aa": "MENKIKTPNKNEEPIIQLVDVVKEFEDKTVLDNVNLSINKGEFITLLGPSGSGKTTILRIIGGFE", "aa_hexdigest": "7f9b71fd612db45245843a321d9ed6c6", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI001E511DD0", "query_cov": 0.9538461538461539, "subject_cov": 0.8493150684931506, "identity": 0.532, "score": 73.6, "evalue": 6.32e-16, "valid": false, "product": "ATP-binding cassette domain-containing protein", "uniref50_id": "UniRef50_UPI00112F2DA6" }, "pscc": { "uniref50_id": "UniRef50_UPI00112F2DA6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00112F2DA6" ], "product": "ATP-binding cassette domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_1027", "locus": "GCA013368035_01026" }, { "type": "cds", "sequence": "contig_1", "start": 593513, "stop": 593872, "strand": "+", "frame": 2, "gene": null, "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "nt": "ATGAACCAAGAAGTATCGGAAATCATGGATCTTGTTGGTTTGAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGTGGTATGAAACAACGTGTTGCACTTGCTCGCTCGCTTGTTATTGAACCTGAAATTCTTCTACTAGATGAACCATTAAGTGCTTTAGATGCTAAAATTAGACAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTTATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATTAGAGATGGTAAGGTTGAACAATTTGCTTCGCCAAAAGATTTATACGATTATCCAATAAACAAATGA", "aa": "MNQEVSEIMDLVGLKGNEKKNVNFLSGGMKQRVALARSLVIEPEILLLDEPLSALDAKIRQKMQTLLRDIQKELKITFIFVTHDRNEALQLSDRIAIIRDGKVEQFASPKDLYDYPINK", "aa_hexdigest": "9efcb0bd6e594589d883b5805109b473", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00237D6FE8", "query_cov": 0.9747899159663865, "subject_cov": 0.9133858267716536, "identity": 0.517, "score": 122.0, "evalue": 8.09e-34, "valid": false, "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_UPI00237D6FE8" }, "pscc": { "uniref50_id": "UniRef50_UPI00237D6FE8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00237D6FE8" ], "product": "ABC transporter ATP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_1028", "locus": "GCA013368035_01027" }, { "type": "cds", "sequence": "contig_1", "start": 593942, "stop": 594184, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGCAAAATTTTCGATACTATTCACAAGGAATTTGAGCCAGGAGAAAACCTTGATGCATTAATTAGACCTGAAGATGTCTATGTTACCAAAGTAAAAGCCAAATCTAAATTTATCGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTAGGTAAAGAAGTATATTTAGATTGA", "aa": "MGKIFDTIHKEFEPGENLDALIRPEDVYVTKVKAKSKFIGKIIKSTYSGSYYNYEVLVKNKIIKMETSINYEVGKEVYLD", "aa_hexdigest": "a4241d06e7d04c26a50daf9835bcc07c", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9271.616300000002, "isoelectric_point": 7.863031578063965 }, "id": "GLJLFKCOHC_1029", "locus": "GCA013368035_01028" }, { "type": "cds", "sequence": "contig_1", "start": 594261, "stop": 594479, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAAAAACAACTGAAATCAACTTCCTATAAAGAAATAGATAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTAGGTGCAAACAAATTGCAACTTTCTTTGCTAATTCCTTATTTAGTATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGCCATCTTGCAAACTGA", "aa": "MEKQLKSTSYKEIDKKTKKSSKFAKFFLGANKLQLSLLIPYLVFSILFIILPLILIFIYAFKPLEGQGHLAN", "aa_hexdigest": "d1327045990832d03897901a28cd46ab", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8277.927599999992, "isoelectric_point": 9.875218772888182 }, "id": "GLJLFKCOHC_1030", "locus": "GCA013368035_01029" }, { "type": "cds", "sequence": "contig_1", "start": 594507, "stop": 595160, "strand": "+", "frame": 3, "gene": "potB", "product": "Spermidine/putrescine ABC transporter permeaseprotein potB", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "nt": "ATGATTATTCTTAGATCAATTGGAACCGGAATATGTGCAGCGTTTTTTGCATTAATCATTGGTTTTCCATATGCCTATATTATTGCAAGAAGTAAATCAATGATATTTAGAACTTTAGGATTAACATTGATTCTAAGCCCGCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTATTTTCGGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCTAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACACAAACTGTTTTTAAAGTTATATTGCCTTACTGCATGAAGGCTATATTGAGTGGTTTTGGAGTTGTTCTTATGATGTCAGCAACATCAATAATTCTTTCAGATAAATTGTTACCAAATGGTAGTCAAAAGCAACTTATAGGAAATCTTATAAATCAGTTTGCCAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTAGCTGTTTTATTAACAATATATGGAATTATTTATGGAATACCTTATTTAATTTCAAAAAGAAAGCAAGGAGGAATATATGAATAA", "aa": "MIILRSIGTGICAAFFALIIGFPYAYIIARSKSMIFRTLGLTLILSPLAIFTISKALAVRGLFSAIFDENELNNNFFLIFGMVYLYIPFMVMPLYQVIKDMPNNIIEASQDLGYSKTQTVFKVILPYCMKAILSGFGVVLMMSATSIILSDKLLPNGSQKQLIGNLINQFANTANPYDLANASTLVIITLAVLLTIYGIIYGIPYLISKRKQGGIYE", "aa_hexdigest": "e85be2a79d3e66b5ba532116f4c918c5", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00352DB7BA", "query_cov": 1.0, "subject_cov": 0.8037037037037037, "identity": 0.843, "score": 357.0, "evalue": 1.38e-122, "valid": false, "product": "ABC transporter permease", "uniref50_id": "UniRef50_D1J8N4" }, "pscc": { "uniref50_id": "UniRef50_D1J8N4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8N4" ], "product": "Spermidine/putrescine ABC transporter permeaseprotein potB" }, "genes": [ "potB" ], "id": "GLJLFKCOHC_1031", "locus": "GCA013368035_01030" }, { "type": "cds", "sequence": "contig_1", "start": 595153, "stop": 595290, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTAAAAAATTTTTGAAGTATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATCATTTTTGGAACAATATATAGTTTTAATGCGCCTTCAGATAAAGGAATATTTTCTGTAACCTCATGA", "aa": "MNKFKKFLKYSYVLIILACLYIPIIFGTIYSFNAPSDKGIFSVTS", "aa_hexdigest": "40b3e6b142f85f76aa021437701af697", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5156.148800000001, "isoelectric_point": 9.519095420837402 }, "id": "GLJLFKCOHC_1032", "locus": "GCA013368035_01031" }, { "type": "cds", "sequence": "contig_1", "start": 595459, "stop": 595710, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "GTGCAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACTGGTTTAACGCTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGGGCAATAATTTCACATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACATGA", "aa": "MQSTSNIPLINPDVITGLTLAIVLNLIFFTTLKATNEGFFRAIISHTVMTLPYGILIMLPKSDKFSKNIFEASQDLGYSKFKT", "aa_hexdigest": "c816bf02c6619802a6f11e59c2631485", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9241.785499999993, "isoelectric_point": 9.157943534851075 }, "id": "GLJLFKCOHC_1033", "locus": "GCA013368035_01032" }, { "type": "cds", "sequence": "contig_1", "start": 595732, "stop": 595863, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTAGGTTCAATAGGATTTACATTTGTAATCACAATGACACTTTCTTTTGATGACTTCATAATTACTAGAATTGTTTCAAATACAGAAACTTTGGGTACTCAATTATATGAAGGTCAATTTCAAGCATGA", "aa": "MLGSIGFTFVITMTLSFDDFIITRIVSNTETLGTQLYEGQFQA", "aa_hexdigest": "2f3fb28131a84edda84bf5710dc59e95", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4807.450900000001, "isoelectric_point": 4.0500284194946286 }, "id": "GLJLFKCOHC_1034", "locus": "GCA013368035_01033" }, { "type": "cds", "sequence": "contig_1", "start": 596014, "stop": 596349, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCTTTATTCCTTGCTTTTTTGGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCCCCAACCATTATTAATAAATTAAAATCAAGATACAATTTCAAAGAATTCAAAGAAATTAGTGAGTTTACTCAAGCATTATTACAAGATAAGGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGAAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGA", "aa": "MRSRILKAISLIFLPLIFIALFLAFLVIKSTNQYRPSIYNYESYLAPTIINKLKSRYNFKEFKEISEFTQALLQDKAIAGIGSDFQAAQLILDRKIQKIDFSLVYGEKYKD", "aa_hexdigest": "b90df7b835b5739ec8301abe712a0fcc", "start_type": "TTG", "rbs_motif": "AGGAG/GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12922.044699999988, "isoelectric_point": 9.583048057556148 }, "id": "GLJLFKCOHC_1035", "locus": "GCA013368035_01034" }, { "type": "cds", "sequence": "contig_1", "start": 596998, "stop": 597114, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACAATGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCAAAAAACACATATATGCTAATGGATAATTGA", "aa": "MYNGDALDAFYSEDNFQSVESGLIRFIRPKNTYMLMDN", "aa_hexdigest": "20c0efe0d8ec23b32898ec4eb584397d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4466.9336, "isoelectric_point": 4.232879447937012 }, "id": "GLJLFKCOHC_1036", "locus": "GCA013368035_01035" }, { "type": "cds", "sequence": "contig_1", "start": 597253, "stop": 597405, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACAAAACAATTAAAACAATCTGCTGAAGCTTCTTTTTTTGAAACCATACATAATATTGACAAAACTTTAAAAAATAAGTTAATTCAAGAATTTCTAAAAAACAAAGAATTTTTCAATATAAACGAAAAATCAACTAGCTTCGAAGAATGA", "aa": "MTKQLKQSAEASFFETIHNIDKTLKNKLIQEFLKNKEFFNINEKSTSFEE", "aa_hexdigest": "c2205a1656575510b7deb76e03c503f0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5968.699199999997, "isoelectric_point": 6.571235466003418 }, "id": "GLJLFKCOHC_1037", "locus": "GCA013368035_01036" }, { "type": "cds", "sequence": "contig_1", "start": 597526, "stop": 597630, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTGATGCTTTTGCAAATAATGAAATAGGAAGTATCTCTAATTTTGATTATGTATCATATACTCCAACTGAAAAAGTAATTTATGATTTTATTAAAAAATGA", "aa": "MSDAFANNEIGSISNFDYVSYTPTEKVIYDFIKK", "aa_hexdigest": "849351753b8e1fb16e3f20c9cd9bc479", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3908.2989000000016, "isoelectric_point": 4.436874580383301 }, "id": "GLJLFKCOHC_1038", "locus": "GCA013368035_01037" }, { "type": "cds", "sequence": "contig_1", "start": 598014, "stop": 598559, "strand": "+", "frame": 3, "gene": "asnS", "product": "asparagine--tRNA ligase", "db_xrefs": [ "COG:COG0017", "COG:J", "EC:6.1.1.22", "GO:0004816", "GO:0005524", "GO:0006421", "KEGG:K01893", "SO:0001217", "UniRef:UniRef50_Q4A9S1", "UniRef:UniRef90_D1J8N7" ], "nt": "ATGCAATTAGTTGTTAAGGGGGATCTAGCATCAAAGAAAGAAATCTCTGAAATTGCCATGGGCTCAGCAGTTAGTGTAATAGGTAAATTACACTTAACGCCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCTCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCGGGAGAAACTTTACTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTTTGGGGGTAACTGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGACCAACATTTAGAGCTGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGA", "aa": "MQLVVKGDLASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEF", "aa_hexdigest": "758f3f7ea52be6bf45cc6c15456d6cb4", "start_type": "ATG", "rbs_motif": null, "genes": [ "asnS" ], "truncated": "both", "pseudogene": { "start": 597873, "stop": 598862, "observations": { "mutation": [ 597942, 598557 ], "stop": [ 597942, 598557 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8N7", "query_cov": 1.0, "subject_cov": 0.40401785714285715, "identity": 0.9940000000000001, "score": 355.0, "evalue": 4.98e-120, "gene_start": 1, "gene_end": 181, "reference_start": 48, "reference_end": 228, "reference_sequence": "MNIKQLYKQISQLQENVELTIEGWIISNRGNDKIRFITINDGSTIANMQLVVKGELASKKEISEIAMGSAVSVIGKLHLTPEAMQPFELSVSKFLYINNPQEDFPIQKKETSLDFLREIPQLRHRTSLFRAIMLIRNSLMFEIHKYFQSKGFLNLSSPIITSNDGEGAGETLLVDDESKDYFFKQKAFLGVTGQLHAESYAEGFKKVYTFGPTFRAENSHTSRHLAEFWMMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKFLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIVKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVDRINELKITQETLQWYLDLRRFGYAPSSGFGLGFERLVMYVTGTSNIRDVIPFPRVAGSIKM" }, "paralog": false, "identity": 0.9878419452887538, "score": 637.0, "evalue": 1.14e-233, "description": "Internal stop codon at 597942, 598557. Nonsense mutation around 597942, 598557.", "psc": { "uniref90_id": "UniRef90_D1J8N7", "gene": "asnS", "product": "asparagine--tRNA ligase", "ec_ids": [ "6.1.1.22" ], "uniref50_id": "UniRef50_Q4A9S1", "cog_id": "COG0017", "cog_category": "J", "kegg_orthology_id": "K01893", "go_ids": [ "GO:0004816", "GO:0005524", "GO:0006421" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8N7", "EC:6.1.1.22", "UniRef:UniRef50_Q4A9S1", "COG:COG0017", "COG:J", "KEGG:K01893", "GO:0004816", "GO:0005524", "GO:0006421" ] }, "pscc": { "uniref50_id": "UniRef50_Q4A9S1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q4A9S1" ], "product": "Asparagine--tRNA ligase" } }, "id": "GLJLFKCOHC_1039", "locus": "GCA013368035_01038" }, { "type": "cds", "sequence": "contig_1", "start": 598560, "stop": 599075, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00152.25" ], "nt": "ATGATGGAACCAGAAGTAGCCTTTTGTGATTTGAAAGGTATCATCGAAATTACAGATGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATATTTGGATTCATTGCAAAACAATAAATTAATTGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGATGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTTGAAGAAAAAGATATCTTTTTTGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCTGAAAACATCATAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTACTAGTACCAGGAATTGGAGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATACCAAAAGCTTGTTAATAGAATTAACGAACTAAAAATCACTCAAGAAACATTACAATGA", "aa": "MMEPEVAFCDLKGIIEITDDMLKNVIANTLKNYPEEMKYLDSLQNNKLIESLNNFINKKLTIIEYKDVVENLKNYVDKFEEKDIFFGMDLASEHEKFLAENIIKGPVAVINYPKDIKAFYMYQNDDGKTVAAFDLLVPGIGELVGGSQRENRYQKLVNRINELKITQETLQ", "aa_hexdigest": "e2b2a2d2e4878c44a4ea87906d392d4f", "start_type": "ATG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00152.25", "name": "tRNA-synt_2", "length": 177, "aa_cov": 0.9824561403508771, "hmm_cov": 0.807909604519774, "evalue": 2.5895925655753855e-21, "score": 71.35741424560547, "start": 1, "stop": 168 } ], "seq_stats": { "molecular_weight": 19764.495599999995, "isoelectric_point": 4.80377025604248 }, "id": "GLJLFKCOHC_1040", "locus": "GCA013368035_01039" }, { "type": "cds", "sequence": "contig_1", "start": 599226, "stop": 599615, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCAAGAAAATAATTTAACAATATTAATTCCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTGTATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCCAAAACCTTTTTCTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATAATGTATTCATATATTGAAATTGAGTATGATTTTATTTCAAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGA", "aa": "MQENNLTILIPIYHPTLTINEILKNLYKQKQQNFNLVIAIDKPSESELYELEKAKTFFSNRLQIIINTNHQHIDSIISSCLNITKTKYTFIMYSYIEIEYDFISKINKLLLEFKDVDIISFDAYTKGIS", "aa_hexdigest": "832ac88b1f4755a0e7b3b13d06f85896", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15202.394499999984, "isoelectric_point": 5.884904670715332 }, "id": "GLJLFKCOHC_1041", "locus": "GCA013368035_01040" }, { "type": "cds", "sequence": "contig_1", "start": 599616, "stop": 599858, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCTAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGGTGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGA", "aa": "MNFNKYPKQNKEINIHIEEENVAKIIPFMYNFIAKTSLLIGATNQHNNKHLSEQYSPNILFKTLINANKILLTKDIQVID", "aa_hexdigest": "30606754ed0a59badd8a4bb2cce4762a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9327.695899999992, "isoelectric_point": 8.965183067321778 }, "id": "GLJLFKCOHC_1042", "locus": "GCA013368035_01041" }, { "type": "cds", "sequence": "contig_1", "start": 599931, "stop": 600116, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCATGAATTTACAACTCCTTTTTCAGAAAGTTATTATTATCTTGCAAAATTTTTAAATATAGCATATTGTTTCGCAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATCGATAAGTGAAAACATTGAAGAATGA", "aa": "MHEFTTPFSESYYYLAKFLNIAYCFAGFLGQCQFKSNTKNYLTLKNLKKHLESSISENIEE", "aa_hexdigest": "67d0416c72beeeb33e93d9cd4cbd7b03", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7182.0805999999975, "isoelectric_point": 6.712196159362793 }, "id": "GLJLFKCOHC_1043", "locus": "GCA013368035_01042" }, { "type": "cds", "sequence": "contig_1", "start": 600575, "stop": 600781, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTTTTATATCTCCCCAAACTTTTGAACAAGCAGCAAATCTTTCTCAAACAAAACCTCTAGATCAACTTCAAAAGGCTTTTCTTTCAATTCCTAGTGTGCTTTCGGCATTAGCCACATTTTTAGAATTCTTATTTATTTTAAGCAATGCCAAGGGAGATAGAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGA", "aa": "MIFISPQTFEQAANLSQTKPLDQLQKAFLSIPSVLSALATFLEFLFILSNAKGDRIGLNDKFSGSYTV", "aa_hexdigest": "ce72db8fc77111110aea6a6a0a21f483", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7483.5468999999985, "isoelectric_point": 6.000572013854979 }, "id": "GLJLFKCOHC_1044", "locus": "GCA013368035_01043" }, { "type": "cds", "sequence": "contig_1", "start": 601006, "stop": 601686, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13086.12", "PFAM:PF13245.12", "PFAM:PF00580.27" ], "nt": "ATGATTGATTTAAAAGATTTAAATGAACAGCAAAAAAGTGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGCGCCGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCGCTAACTTTTTCAAATAAGGCTGCAAACGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCTTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTATTCAGAACTTGATATTTCACCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAACGATAATAACGAACATTTTAATGAAGACTCAGATTCACAAGAAACACAAGATGAAAAAATCAAGGCAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTCGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTATTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCTGAAAGATGA", "aa": "MIDLKDLNEQQKSAVIYNEGPLRIIAGAGSGKTRVLTYKIAYLIQKLGVLPNRILALTFSNKAANEMKQRVFNLLAEDNPSNLSPYISTFHAMCAKILRKEIYNFGYENDFQILDELDQKEILKIVYSELDISPTEFTFSSIISFIQNKKNSIEYLNNSINDNNEHFNEDSDSQETQDEKIKAIKEKIYERYQLHLERSHSLDFDDLLVFVYKLFYEPKFSFVAER", "aa_hexdigest": "c8c0bd3435086c36cf028080ebc86a62", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13086.12", "name": "AAA_11", "length": 199, "aa_cov": 0.827433628318584, "hmm_cov": 0.8944723618090452, "evalue": 1.6316971088526832e-08, "score": 29.98868179321289, "start": 7, "stop": 193 }, { "id": "PF13245.12", "name": "AAA_19", "length": 123, "aa_cov": 0.5309734513274337, "hmm_cov": 0.5853658536585366, "evalue": 3.6217723381915625e-14, "score": 48.503517150878906, "start": 11, "stop": 130 }, { "id": "PF00580.27", "name": "UvrD-helicase", "length": 218, "aa_cov": 0.9336283185840708, "hmm_cov": 0.9220183486238532, "evalue": 2.692094041066698e-50, "score": 166.92657470703125, "start": 7, "stop": 217 } ], "seq_stats": { "molecular_weight": 26346.566800000026, "isoelectric_point": 5.243078804016112 }, "id": "GLJLFKCOHC_1045", "locus": "GCA013368035_01044" }, { "type": "cds", "sequence": "contig_1", "start": 601843, "stop": 602046, "strand": "+", "frame": 1, "gene": null, "product": "3'-5' exonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "nt": "ATGAATTTTGATAAGGATTACCCAAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATCTTAAGAGCAGCAAACAACTTAATTTCGCATAATAAACTTAGGTTAGAAAAGAAATTATTCACTGAAAACGAAGAAGGCGAAGATATCGAGTTTTATTGTGGTTTTAATGACGAAGCAGAAGCTAGATGA", "aa": "MNFDKDYPNSVTVKLEENYRSTKTILRAANNLISHNKLRLEKKLFTENEEGEDIEFYCGFNDEAEAR", "aa_hexdigest": "97549997a635dfe7cb9efc2ab9e63df3", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00273A74DA", "query_cov": 1.0, "subject_cov": 0.8701298701298701, "identity": 0.5670000000000001, "score": 73.9, "evalue": 5.36e-16, "valid": false, "product": "3'-5' exonuclease", "uniref50_id": "UniRef50_UPI00273A74DA" }, "pscc": { "uniref50_id": "UniRef50_UPI00273A74DA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00273A74DA" ], "product": "3'-5' exonuclease" }, "genes": [], "id": "GLJLFKCOHC_1046", "locus": "GCA013368035_01045" }, { "type": "cds", "sequence": "contig_1", "start": 602719, "stop": 603243, "strand": "+", "frame": 1, "gene": null, "product": "DNA 3'-5' helicase II", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "nt": "ATGACTGTTCACAACTCAAAAGGGCTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAATATCTTTCCATTAAAGAGGGCCATATCGATTTCTCCACAAGACGATTTTACTTTTTTAAATTCAAAAAATTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGGCGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCAGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTCTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATTTAATAGTCGGTGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGATGATGAAAGCCTAAATAGTTAA", "aa": "MTVHNSKGLEFDYVFVACLAENIFPLKRAISISPQDDFTFLNSKNFKENIEGLEEERRLAYVAMTRAKKRLMLSFSVGRNGINRKSRFLSEAGINEIRTIKIANNFSIAAEQETRNNDLIVGDYIIHNTYGKGLIIDMVDNVIEVKFENDKKIRKLSKTHHSIKKYEDDESLNS", "aa_hexdigest": "22892f36b61691374efe89fa7b9e4227", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEJ6", "query_cov": 0.9597701149425287, "subject_cov": 0.8835978835978836, "identity": 0.6409999999999999, "score": 207.0, "evalue": 2.45e-65, "valid": false, "product": "DNA 3'-5' helicase II", "uniref50_id": "UniRef50_A0A0F3QEJ6" }, "pscc": { "uniref50_id": "UniRef50_A0A0F3QEJ6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F3QEJ6" ], "product": "DNA 3'-5' helicase II" }, "genes": [], "id": "GLJLFKCOHC_1047", "locus": "GCA013368035_01046" }, { "type": "cds", "sequence": "contig_1", "start": 603689, "stop": 603871, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGATAATGTTTTCAATAAAGAAATTCATGATTTGTCTTATATTCCAACCATTGAAAATTATGAAGGCTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATGCTTTTTTAGATCTGTTTAAAGATTTAGGAAAACAACACAATATTAATGGCTTAGAAGTTGTGTATGAATGA", "aa": "MDNVFNKEIHDLSYIPTIENYEGLFFFLNKKHITDINAFLDLFKDLGKQHNINGLEVVYE", "aa_hexdigest": "2f4d50a3afdb6ea7115de8983dfb50be", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7118.981400000001, "isoelectric_point": 4.923245811462402 }, "id": "GLJLFKCOHC_1048", "locus": "GCA013368035_01047" }, { "type": "cds", "sequence": "contig_1", "start": 604465, "stop": 604659, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAGGTTCGGTTAATCTAGAGGTTAGTTCTTCATCTATTCAAATGAATTTAACTCAAGGGGATACCAGAGAAATTATTGTTGAATCAATATCCTTAATTTCGAGACAAAAATTGAGTTTTGTTCCTTGTAGTTTAACAATTTTTTGGGATATGTCTTTTATTCTTTCTGCTGATAATAATTTTATTGATTGA", "aa": "MLGSVNLEVSSSSIQMNLTQGDTREIIVESISLISRQKLSFVPCSLTIFWDMSFILSADNNFID", "aa_hexdigest": "134f2509d5cb5e85eff095020be02482", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7143.107599999997, "isoelectric_point": 4.261810493469238 }, "id": "GLJLFKCOHC_1049", "locus": "GCA013368035_01048" }, { "type": "cds", "sequence": "contig_1", "start": 604844, "stop": 604999, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAGTAAATAAAAAGTGGGTAGATAGCCATAAAATCCCTGATTCTAAAACCTCTATAGGTTGCTTTGCGCAATTAGATAAAAATATTACTACATTAACTAAAAAAATATTAAATACATGA", "aa": "MNKKLIPIDFFDAVNKKWVDSHKIPDSKTSIGCFAQLDKNITTLTKKILNT", "aa_hexdigest": "e7879c837e3af9107b8cc9a726466674", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5820.7789999999995, "isoelectric_point": 9.519159889221193 }, "id": "GLJLFKCOHC_1050", "locus": "GCA013368035_01049" }, { "type": "cds", "sequence": "contig_1", "start": 605171, "stop": 605338, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAACTCTTTATTTAGATGTAAACTCTACAATTCTACCAGACAAAAGTTTTTATACCGATCCTGCCAAAAAAGAAAAATTTATTAATGTTTGA", "aa": "MELKLSSLNTPLIFSVSTDFKNSDVQTLYLDVNSTILPDKSFYTDPAKKEKFINV", "aa_hexdigest": "d4ebd3015aa40d7eb1610e9a71230fb9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6257.0754000000015, "isoelectric_point": 4.983893013000488 }, "id": "GLJLFKCOHC_1051", "locus": "GCA013368035_01050" }, { "type": "cds", "sequence": "contig_1", "start": 605471, "stop": 606145, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAATTTGCAACACTATTTCAACAAAAGAATTAGCCCAAAACATTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCCGATGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAGTCTTTAACCAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTTGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACGTTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACAGTTGAACACATGATAAAAAGTATGATAGATGTTTATAAAGAACAACTAACAAAAAACTCTTGGTTAAGTAAGCAAACAAAAGAAAAAGCAATAATAAAACTTAACTCAATAGGTGTTTACGTTGGCTTTCCTGAAAAAATTCAAGATCACTATAAATATTTAATAGTTGAAGAATTTGATGGATACAATGATTTATTTGAAAATGTTTTAAAATTTAACAGAATTTTAGCAGAACACATCTTATCCGAATATGGAAAAACAGAAGACAAAAACCTTTGA", "aa": "MVKICNTISTKELAQNIKTINIENILRQLIKCFPDEINAIDPNSINIAKSLTNEENFEYYKASLIIDAILTYAPYFDYSTIQIASEFNLAINGNKKIESKEKYQIKQAKRFFDIPFGTYYGKTYFGLKNKQTVEHMIKSMIDVYKEQLTKNSWLSKQTKEKAIIKLNSIGVYVGFPEKIQDHYKYLIVEEFDGYNDLFENVLKFNRILAEHILSEYGKTEDKNL", "aa_hexdigest": "a9cb55d9936b5d6ee78956e5d8358dd8", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 26164.746200000027, "isoelectric_point": 7.639296913146972 }, "id": "GLJLFKCOHC_1052", "locus": "GCA013368035_01051" }, { "type": "cds", "sequence": "contig_1", "start": 606149, "stop": 606358, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCGCCTGCTATGGTTAACGCCTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCTGCAATCTTGCAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTCGGCGGAATTGGTGCTGTTATTGCCCATGAAATATCACACGGATTTGACAATAACGGAGCAAATTTCGACGAAAAAGGAAATATGATTAATTGA", "aa": "MSPAMVNAYYNPSKNVIVFPAAILQKPFFSADQSSSSNFGGIGAVIAHEISHGFDNNGANFDEKGNMIN", "aa_hexdigest": "6ebac6a6f366996c081c11a5317a49ee", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7351.097399999999, "isoelectric_point": 5.337602043151855 }, "id": "GLJLFKCOHC_1053", "locus": "GCA013368035_01052" }, { "type": "cds", "sequence": "contig_1", "start": 606404, "stop": 606574, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTAATTTATTTGAAGGTAGAGAAACATCTGTTGGTAAATGTAATGGAACATTAACCGTTTCAGAAAACATAGCAGATGCTGGTGGATTATCGTGTGCTTTAGCAGCAGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGA", "aa": "MINLFEGRETSVGKCNGTLTVSENIADAGGLSCALAAAKLEKDYNPKDFYINFATI", "aa_hexdigest": "8a342e6c8964b587198068ef7141ae43", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5986.715900000001, "isoelectric_point": 4.715044593811036 }, "id": "GLJLFKCOHC_1054", "locus": "GCA013368035_01053" }, { "type": "cds", "sequence": "contig_1", "start": 606790, "stop": 607701, "strand": "-", "frame": 2, "gene": "tmcAL", "product": "nucleotidyltransferase", "db_xrefs": [ "COG:COG1323", "COG:J", "EC:6.3.1.22", "KEGG:K27343", "RefSeq:WP_175949962.1", "SO:0001217", "UniParc:UPI00158EE0E3", "UniRef:UniRef100_UPI00158EE0E3", "UniRef:UniRef50_A0A3M0A6W2", "UniRef:UniRef90_D1J8P3" ], "nt": "ATGAAAATAGGAATAATAGCTGAATTTAACCCATTTCACAATGGGCATATTTATTTAATTAATAAAATTAAAGAAATCTTCAAAGATCCTGAAATAATTGTTGCATTATCTTGCGATTATGTTCAACGGGGCGAAATAGCATGTTTACCTTTTGAAGTTAGAAAAAACATTGCGCTTGAATATGGAGCAACAAAAGTTGTTGAATTAGATTTTTTTGCATCTACACAAGCAGCCCATATTTTTGCTAAAAAATCAATAGATTTGTTAATAAAAGAAGGTATTGATTATTTAGTTTTTGGGGTTTCTGACACTGATGATATAAAAAAATATTTAAACGCAGCAAACGTAATAAAGAAAAATTTTGATCAATATAACAAAGATGTAAGAATGAATTTAAAAACAGGAAAATCATATGTTTTGAGTTGTTTCTTGTCGCTAGAAAAATTAATAGGTGTTGAAAATATACCACAAGATATTTTAGGGTTTGAATATACCAAATATATAGTTTTTAATAATTTATCAGTAAAACCGTTTTGCATAAAAAGAACAGCTCCACACAATTCGTTAATAGCAAATAATAATTATGCTTCGGCAACAATGATACGAAAAATGTTAGAAGAAGGCGAAGATGTTTCAATGTTCACACCGATAAACATTCCTAAAAATTTTTCAAAAATTGAAGATAAATATAATGAGTTTAAACAAAAGGTACAAAATTCTTCTAAAGAAGAACTAGCAAATATAGCATTAATATCCGAAGGAATGGAAAATCTTTTTAAAAAGAATATTGAAATAGCAAAAAATTATAATGAATTTGTTGATTTATGTACTTCGAAAAGATATACAAATAGCAGAATTAAACGAGTGATGCTGTATGTTCTTTTAGGAATAAAGAAAGAAGATTTGTGTTAA", "aa": "MKIGIIAEFNPFHNGHIYLINKIKEIFKDPEIIVALSCDYVQRGEIACLPFEVRKNIALEYGATKVVELDFFASTQAAHIFAKKSIDLLIKEGIDYLVFGVSDTDDIKKYLNAANVIKKNFDQYNKDVRMNLKTGKSYVLSCFLSLEKLIGVENIPQDILGFEYTKYIVFNNLSVKPFCIKRTAPHNSLIANNNYASATMIRKMLEEGEDVSMFTPINIPKNFSKIEDKYNEFKQKVQNSSKEELANIALISEGMENLFKKNIEIAKNYNEFVDLCTSKRYTNSRIKRVMLYVLLGIKKEDLC", "aa_hexdigest": "d8850cb62c2dc7b4f700efac2526ab1d", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158EE0E3", "ncbi_nrp_id": "WP_175949962.1", "uniref100_id": "UniRef100_UPI00158EE0E3", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158EE0E3", "RefSeq:WP_175949962.1", "UniRef:UniRef100_UPI00158EE0E3" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158EE0E3", "uniref90_id": "UniRef90_D1J8P3" }, "psc": { "uniref90_id": "UniRef90_D1J8P3", "gene": "TmcAL", "product": "nucleotidyltransferase", "ec_ids": [ "6.3.1.22" ], "uniref50_id": "UniRef50_A0A3M0A6W2", "cog_id": "COG1323", "cog_category": "J", "kegg_orthology_id": "K27343" }, "pscc": { "uniref50_id": "UniRef50_A0A3M0A6W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3M0A6W2" ], "product": "tRNA(Met) cytidine acetate ligase" }, "genes": [ "tmcAL" ], "id": "GLJLFKCOHC_1055", "locus": "GCA013368035_01054" }, { "type": "cds", "sequence": "contig_1", "start": 607762, "stop": 607917, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58", "UniRef:UniRef90_A0A454CA58" ], "nt": "ATGTTTAATAAAAAGAAAACAAGCAAGAAAAAACAAATAAAAAAAGAAAAAGAACAAAGCGATATTGTGATGTTCGAAGATTTTGATTCAAACAAAGATTGTGAAGGCGATCAAAAAAACATTGAGATCCAAGAAACTGTGGAATTAAATTATTGA", "aa": "MFNKKKTSKKKQIKKEKEQSDIVMFEDFDSNKDCEGDQKNIEIQETVELNY", "aa_hexdigest": "e7f38b5d3faae0d5a5609b5fba976c54", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 607702, "stop": 607917, "observations": { "mutation": [ 607764 ], "stop": [ 607764 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A454CA58", "query_cov": 1.0, "subject_cov": 0.7183098591549296, "identity": 0.902, "score": 84.0, "evalue": 2.56e-20, "gene_start": 1, "gene_end": 51, "reference_start": 1, "reference_end": 51, "reference_sequence": "MLNKKKTNKKKQIKKEKEQSDIVMFEDFDSNKKSEDDQKNIEIQETVELNYWEDSSIEDFCDISLDELKKK" }, "paralog": false, "identity": 0.9154929577464789, "score": 129.0, "evalue": 6.46e-41, "description": "Internal stop codon at 607764. Nonsense mutation around 607764.", "psc": { "uniref90_id": "UniRef90_A0A454CA58", "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A454CA58", "UniRef:UniRef50_A0A454CA58" ] }, "pscc": { "uniref50_id": "UniRef50_A0A454CA58", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CA58" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 6130.8432999999995, "isoelectric_point": 5.356643104553222 }, "id": "GLJLFKCOHC_1056", "locus": "GCA013368035_01055" }, { "type": "cds", "sequence": "contig_1", "start": 608138, "stop": 609214, "strand": "+", "frame": 2, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002359FB52" ], "nt": "ATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGCAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAAGATTCAATTGAGATTGTTAAAAAAGTTTTTAGTGGTAAAGTTGGAAAAGATAAGACAATTACTTACGGAAATATCATTGATAATATAACAGGAAAAATCGTTGATGAGGTATTAGTTAATTGGTTTATTGGTAATAAAAATTATACTGGAGAAAATACCGTTGAAATAAATGCTCATGGTGGAATTGTTGTAACAAACAAAATACTTAATCTATTAATTGCTAATGGTGCAAGGTTGGCAAACAGAGGAGAATTTACAAGGCGAGCATTTTTAAACGGAAAAATTTCGTTAGATAAAGCCGAAGCGATAAATAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATTAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAGTCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATAAATGTTGCGATAGTGGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTTTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCCGGAACAACAAGAGACGTGGTAGAAGGTGAATTTCAGATTAATGGTTTTTTATTTAATTTGATTGATACAGCGGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATTGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATAAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATACCAGTAATGAATAAGCAAGATCTTTTAAATAAAGATCAATATCCTAAGAATATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGA", "aa": "MKNNIQDTIVAISSGNVNQAISIIRLSGEDSIEIVKKVFSGKVGKDKTITYGNIIDNITGKIVDEVLVNWFIGNKNYTGENTVEINAHGGIVVTNKILNLLIANGARLANRGEFTRRAFLNGKISLDKAEAINSLIHAKTNKQAEIAISQFNSKDNLIIENLINELLTIISLIEINIDYSDYNDIKEMDANTLTSQLSKFNRKLDSIINKSENACDIYKGINVAIVGRPNTGKSSLLNSLIGSQKAIVTNIPGTTRDVVEGEFQINGFLFNLIDTAGIRQTKDLVESIGIERSKNAIKEAKIVIHMHEPFIKENEEDKIIKELSKGKVYIPVMNKQDLLNKDQYPKNMVLISSKENSL", "aa_hexdigest": "be9033d188de82d9a5dd8d1ec65d4f65", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002359FB52", "query_cov": 0.9888268156424581, "subject_cov": 0.9915966386554622, "identity": 0.573, "score": 379.0, "evalue": 1.34e-127, "valid": false, "gene": "mnmE", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE", "uniref50_id": "UniRef50_UPI002359FB52", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006400" ] }, "pscc": { "uniref50_id": "UniRef50_UPI002359FB52", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI002359FB52" ], "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE" }, "genes": [ "mnmE" ], "id": "GLJLFKCOHC_1057", "locus": "GCA013368035_01056" }, { "type": "cds", "sequence": "contig_1", "start": 609245, "stop": 609397, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAGCTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCACTAGAATCACTAAGTCAAGGTTTAGGCCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGA", "aa": "MNIDLDDPQIIFNNRKLSLLKQAKISINTALESLSQGLGPEVVILDINEA", "aa_hexdigest": "2500abab43290ee70e2b4b9032c79911", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A0F3QEF2", "query_cov": 1.0, "subject_cov": 0.9090909090909091, "identity": 0.62, "score": 69.7, "evalue": 7.25e-15, "valid": false, "product": "Catalytic cysteine-containing of GTPase, MnmE family protein", "uniref50_id": "UniRef50_A0A3B0P0P4" }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5506.285699999999, "isoelectric_point": 4.385492134094237 }, "id": "GLJLFKCOHC_1058", "locus": "GCA013368035_01057" }, { "type": "cds", "sequence": "contig_1", "start": 609486, "stop": 610040, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTATAAAATAAACGACAAAATAGTTGTGAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTAAGAAGTGATAAACAAACACTTTTTGTTGAAAATCTCTTACCTGATGAAGAAGCAGAAGTAGCCATTTATAAATCAAATTCAAAAATTGCTTATGGACGTACAGTTAATTTGATAAAAAAATCATGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTGTTTGAAAGTGGTGCAGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTCAAAACATCGAACAAATTCAACCTAGTCCAAAAATATTAAATTATAGAAATAAAATAACCTTACATGTGAATTATAAAAACAATAATATTTATATTGGTTTCTATAAAAAAAACAGCAACAAACTAGTTATTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAAGAACTTATTAGTGCCCTAAAACAACAAATTTTATCAAAAGATAATTGA", "aa": "MYKINDKIVVKATELSYEGYGVVRSDKQTLFVENLLPDEEAEVAIYKSNSKIAYGRTVNLIKKSCKRVEVVEKELFESGAAPLMVLSYEDQLKFKQEIVNNLIKRNIGFQNIEQIQPSPKILNYRNKITLHVNYKNNNIYIGFYKKNSNKLVIQNSLPLCHDELDKYYKELISALKQQILSKDN", "aa_hexdigest": "63fc883b6cff312bec33e74aa9d2884b", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 21369.421800000026, "isoelectric_point": 9.09727878570557 }, "id": "GLJLFKCOHC_1059", "locus": "GCA013368035_01058" }, { "type": "cds", "sequence": "contig_1", "start": 610149, "stop": 611099, "strand": "+", "frame": 3, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F5D49A8" ], "nt": "TTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAATATATTAAAGAATCTAAAGATATATTCACTTCTTTGTATAAATCAATGCCACAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCAACAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCGGCGTGTGAGTGTGCAAAAATTAACATAAACCAAAATTTGATTAATCCAAAACTAGCGGATATAACAAATAGTTCAGCACAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCGATAGTTATATTTGACCCACCAAGAACGGGTTTAACAAATAAAGAAATTGATATGCTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGAATCGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAGACGGTAGCACTTTTGTCCAAACTTGATGTCGATAAGCATATAAATATTGAAATTGAGCTGGATGAGCTTGATTTGACAAGTGCTGAAAGTAAGGCAACATATGCTCAAATCAAAGAATATGTTTGGAATAAATTTGAATTAAAAGTTCCGACATTATATATTGCACAGATAAAAAGGAAATGTGGAATAGAATTACGAGAACATTACAACAAGTCTAAAAAGGAGAAACAAATTATTCCACAGTGTACACCTGAAAAAGAAGAAGCTATCATGGATGCCTTGAGACACTTCAAAATGATTGAATAG", "aa": "MKNNLIFLLKPNVISIQCQYIKESKDIFTSLYKSMPQQFNINNKIFNVNNDSFFQVNEEQTSLIYNQIKKFIDQENSTKIIDVYSGVGSIGISVCGNDSQLELIEINKSACECAKININQNLINPKLADITNSSAQDFFNKKEAEYFEKSIVIFDPPRTGLTNKEIDMLEKCNSVIYMSCNPHTMIRDLKYFEEKGYRIERLIPYDMFPQTYHIETVALLSKLDVDKHINIEIELDELDLTSAESKATYAQIKEYVWNKFELKVPTLYIAQIKRKCGIELREHYNKSKKEKQIIPQCTPEKEEAIMDALRHFKMIE", "aa_hexdigest": "9ddf5c0baed5d4a98f2a61123e010b96", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI000F5D49A8", "query_cov": 1.0031645569620253, "subject_cov": 0.8879551820728291, "identity": 0.502, "score": 283.0, "evalue": 1.41e-90, "valid": false, "gene": "rlmD", "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD", "ec_ids": [ "2.1.1.190" ], "uniref50_id": "UniRef50_UPI000F5D49A8", "kegg_orthology_id": "K03215", "go_ids": [ "GO:0008649" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000F5D49A8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000F5D49A8" ], "product": "23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD" }, "genes": [ "rlmD" ], "id": "GLJLFKCOHC_1060", "locus": "GCA013368035_01059" }, { "type": "cds", "sequence": "contig_1", "start": 611453, "stop": 611614, "strand": "+", "frame": 2, "gene": "ardA", "product": "antirestriction protein ArdA", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DB066", "UniRef:UniRef90_UPI00215DB066" ], "nt": "ATGTATGACGTGGCACGCTACTACATTGAAGAAACGGGTGCTTTAGGCGAAGTACCAGCTAGTCTTCAAAACTATATTGATTATCAAGCCTATGGTCGGGATTTAGACCTTTCAGGAACGTTTATCTCAACCAATCATGGGATTTTTGAAATCGTCTATTAA", "aa": "MYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGIFEIVY", "aa_hexdigest": "f5eb63f05aea186577acbf7cc66d002f", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00215DB066", "query_cov": 0.9622641509433962, "subject_cov": 0.8095238095238095, "identity": 1.0, "score": 107.0, "evalue": 1.1e-29, "valid": true, "product": "antirestriction protein ArdA", "uniref50_id": "UniRef50_UPI00215DB066" }, "pscc": { "uniref50_id": "UniRef50_UPI00215DB066", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00215DB066" ], "product": "antirestriction protein ArdA" }, "genes": [ "ardA" ], "id": "GLJLFKCOHC_1061", "locus": "GCA013368035_01060" }, { "type": "cds", "sequence": "contig_1", "start": 611589, "stop": 612095, "strand": "+", "frame": 3, "gene": null, "product": "Conjugative transposon membrane protein", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B7173", "UniRef:UniRef100_A0A0G2PMG1", "UniRef:UniRef50_Q832E9", "UniRef:UniRef90_C2BCT9" ], "nt": "ATGGGATTTTTGAAATCGTCTATTAAATCTGTCGGTACATTACTACTGGCAGATTTTCTATTTTACGGGGTGGCTCAATCAGCTACCCCTATTTTTTATGAAAGGATTGATTACATGAAGAAAATACGAAGCTATACCAGTATCTGGTCTGTGGAAAAGGTACTGTATTCTATCAATGATTTTAGACTTCCGTTTCCCATAACCTTTACGCAAATGACATGGTTTGTCGTGTCACTCTTTGCAGTGATGATACTTGGCAACTTGCCCCCTCTTTCCATGATAGAGGGAGCATTTCTCAAATACTTTGGGATTCCTGTGGCTTTCACATGGTTTATGTCTACAAAAACTTTTGATGGTAAAAAGCCTTATGGATTTTTGAAGTCTGTCATTGCTTATGCACTGCGACCAAAGCTGACCTATGCAGGAAAAAAAGTAACGCTTGGCAGAAACCAGCCACAAGAAGCCATTACAGCAGTTAGGAGTGAATTTTATGGCATATCCAATTAA", "aa": "MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVRSEFYGISN", "aa_hexdigest": "afbc1c34e35a5ffa43888b47dc39e41a", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000B7173", "uniref100_id": "UniRef100_A0A0G2PMG1", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B7173", "UniRef:UniRef100_A0A0G2PMG1" ] }, "ips": { "uniref100_id": "UniRef100_A0A0G2PMG1", "uniref90_id": "UniRef90_C2BCT9" }, "psc": { "uniref90_id": "UniRef90_C2BCT9", "product": "Conjugative transposon membrane protein", "uniref50_id": "UniRef50_Q832E9" }, "pscc": { "uniref50_id": "UniRef50_Q832E9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q832E9" ], "product": "Conjugal transfer protein" }, "genes": [], "id": "GLJLFKCOHC_1062", "locus": "GCA013368035_01061" }, { "type": "cds", "sequence": "contig_1", "start": 612079, "stop": 613953, "strand": "+", "frame": 1, "gene": null, "product": "ATP-binding protein", "db_xrefs": [ "RefSeq:WP_175949964.1", "SO:0001217", "UniParc:UPI00158E92B4", "UniRef:UniRef100_UPI00158E92B4", "UniRef:UniRef50_F7KH32", "UniRef:UniRef90_UPI0005E3455D" ], "nt": "ATGGCATATCCAATTAAATACATTGAAAACAATCTCGTCTGGAATAAAGACGGGGAATGTTATGCTTACTATGAGCTTGTTCCTTACAATTACTCATTTCTAAGTCCAGAACAGAAAATACAAGTGCATGATTCTTTCAGACAGCTTATCGCACAAAATCGTGATGGCAAAATTCATGCTTTACAAATCAGTACAGAATCCAGCATACGTTCTGCACAAGAGCGTTCCAAAAATGAAGTCACTGGCAAGCTCAAAGCGGTTGCCTATGACAAAATCGACCAACAGACAGACGCTTTAATATCCATGATTGGCGAAAATCAAGTGAACTACCGTTTCTTTATCGGCTTTAAGTTGCTTCTCAACGATCAGGAGTTTTCTATGAAAAGTCTTACCGTTGAAGCAAAAAATGCTTTGTCTGATTTTGTCTATGATGTGAACCATAAGCTGATGGGCGATTTTGTTAGTATGAGTAATGATGAAATCCTGCGTTTTCAGAAGATGGAAAAGCTCTTAGAAAATAAAATCTCTCGTCGTTTCAAAATCCGCAGGTTAGATAAGGACGACTTCGGCTATCTGATTGAACACCTTTACGGACAGACAGGCACTGCCTATGAAGAGTATGAGTACCATCTATCAAAGAAAAAGCTGGATAATGAAACGCTGATTAAATACTATGACTTGATTAAGCCTACTCGCTGTTTGGTGGAAGAAAAACAGCGATATTTGAAAATCCAGCAGGAAGATGAAACCGTCTATGTAGCTTACTTTACCATTAACAGCATTGTCGGAGAACTGGACTTCCCGTCCTCTGAAATCTTCTACTACCAGCAACAGCAATTTACATTCCCGATTGATACGTCAATGAATGTGGAAATTGTAGCGAATCGTAAAGCCCTATCTACTGTCCGCAATAAAAAGAAAGAACTGAAAGACTTGGATAACCACGCTTGGCAAAGTGATAATGAAACCAGCTCCAATGTGGCGGAAGCTCTGGAAAGTGTGAATGAGTTGGAAACCAATTTAGACCAAAGCAAGGAATCTATGTACAAGCTGTCTTATGTGGTAAGGGTATCAGCAAATGATCTTGACGAACTCAAACGTCGTTGTAATGAAGTGAAAGATTTTTATGACGATTTAAGCGTAAAACTGGTACGACCATTTGGGGATATGCTCGGCTTACATGAAGAATTTTTACCTGCCAGCAAGCGTTATATGAATGATTATATTCAATACGTGACCTCTGATTTCCTCGCTGGTTTAGGTTTTGGTGCTACTCAAATGCTGGGGGAAAATGAGGGGATTTATGTTGGCTACAGCTTAGAAAAACAACTGAACATTATACAGGTTGCGGACTTGGTACTTCCCGACAAGGAAACTTCCTTTGAGGAATATACCACTATGGAGCTTTTATCCGTTGCTATGCTGATTGTCATTAGTACCTTTGCTTTAGACTTTATCCATACAGACCGAAGCATTTTCAAGATTGTAGATTTAGACGAAGCATGGAGCTTTTTACAGGTAGCACAAGGAAAAACACTATCTATGAAGCTGGTTCGGGCTGGTCGTGCTATGAACGCTGGGGTATATTTCGTGACCCAAAATACAGACGACCTCTTAGATGAAAAACTGAAAAATAACCTCGGCTTAAAATTTGCATTTCGTTCCACTGACCTTAACGAGATTAAAAAGACCTTAGCCTTTTTTGGTGTAGACCCAGAGGACGAAAACAATCAGAAGCGATTGCGTGATTTGGAAAACAGGCAATGCCTTATCAGTGATTTATATGGTCGTGTCGGTGTGATACAGTTCCACCCTGTATTTGAAGAACTGCTCCATGCCTTTGATACCAGACCACCTGTGCGAAAAGAGGTGTAA", "aa": "MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHDSFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISMIGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDEILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDNETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQQQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESVNELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLGLHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENRQCLISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV", "aa_hexdigest": "e555fa771d732c33812211c90d958534", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158E92B4", "ncbi_nrp_id": "WP_175949964.1", "uniref100_id": "UniRef100_UPI00158E92B4", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E92B4", "RefSeq:WP_175949964.1", "UniRef:UniRef100_UPI00158E92B4" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E92B4", "uniref90_id": "UniRef90_UPI0005E3455D" }, "psc": { "uniref90_id": "UniRef90_UPI0005E3455D", "product": "ATP-binding protein", "uniref50_id": "UniRef50_F7KH32" }, "pscc": { "uniref50_id": "UniRef50_F7KH32", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F7KH32" ], "product": "ATP/GTP-binding protein" }, "genes": [], "id": "GLJLFKCOHC_1063", "locus": "GCA013368035_01062" }, { "type": "cds", "sequence": "contig_1", "start": 613956, "stop": 616133, "strand": "+", "frame": 3, "gene": "ytxH", "product": "YtxH domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1BBB6", "UniRef:UniRef90_A9J076" ], "nt": "GTGAAACCATCAATAGTAAACAGAATAAAATCAAACTGGACGCTGAAACGTCTAGGTAAAGTGGCAATGACAGTGGCTTTCACACTTGTGATTGCCATTTTTCTTTTAGCCATGCTGGGAACGGTGGTTCAAGCTGCGGGCTTGGTAGATGATACGGTCAATGTGGCAAATGAATACAGCCGATACCCACTTGAAAACTATCAACTGGATTTTTATGTGGATAATAGCTGGGGCTGGCTTCCGTGGAACTGGTCGGACGGGATTGGAAAACAGGTCATGTATGGACTATATGCCATTACCAATTTTATTTGGACAATCAGTTTGTATGTTTCCAATGCGACAGGTTACTTAGTACAGGAAGCCTATTCCTTAGACTTCATTTCCGCTACAGCAGATTCCATTGGTAAGAATATGCAGACCTTAGCTGGTGTGAGTGCAAACGGATTTTCAACAGAGGGTTTCTATGTTGGATTCCTCTTACTCTTGATTTTGGTTCTTGGGGTTTATGTTGCCTATACGGGACTGATAAAGAGAGAAACCACAAAGGCAATTCATGCCATTATGAATTTTGTGCTGGTGTTTATCCTATCGGCTTCCTTTATTGCCTACGCTCCCGACTACATTAAAAAAATCAATGACTTTTCATCAGACATCAGTAATGCCAGTTTATCACTTGGCACGAAGATTGTCATGCCCCATTCCGATAGTCAAGGCAAGGACAGCGTGGACTTAATCAGAGATAGCCTGTTTTCCATACAGGTTCAGCAACCGTGGCTACTGCTTCAATACAACAGTTCAGACATTGAAAGTATCGGTATTGACCGTGTGGAAAGCCTGCTCTCCACCAGCCCAGATTCCAACAATGGCGAAGACAGAGAAAAAATTGTTGCGGAAGAAATTGAAGACAGAAGCAATACCAATCTAACCATTACAAAGACCATTAACCGTTTAGGTACAGTCTTCTTCCTATTTGTCTTCAATATTGGGATTTCCATATTTGTATTCCTATTAACAGGAATCATGATTTTCTCGCAGGTACTTTTCATCATCTATGCTATGTTTCTGCCTGTGAGCTTTATTTTAAGCATGATTCCATCATTTGATGGTATGTCAAAACGAGCCATAACAAAGCTCTTTAATACCATTTTGACACGAGCTGGAATCACATTGATTATTACGACAGCATTTAGTATTTCAACCATGCTCTATACCTTATCGGCTGGTTATCCGTTCTTTTTGATTGCTTTTCTACAGATTGTGACCTTTGCAGGAATCTACTTCAAGCTGGGCGATTTAATGAGTATGTTTTCTCTACAGAGTAACGATTCTCAAAGTGTGGGAAGTCGTGTGATGAGAAAACCTCGTATGCTTATGCACGCTCACATGCACCGTCTACAGCGGAAACTTGGACGTTCCATGACTACTCTAGGGGCTGGGTCTGCCATTGTTACAGGTAAAAAAGGACAGTCGGGTTCGGGGAGTTCTGCAAGGACACAAGCAGATCACTCCCGACCAGACGGAAAGGAAAAATCAACACTTGGAAAACGTATCGGTCAAACCATCGGTACAGTAGCTGATACCAAAGACAGAATGGTAGACACTGCTAGTGGTTTGAAAGAACAGGTTAAAGATTTGCCGACCAATGCAAGATATGCAGTATATCAAGGAAAATCCAAAGTAAAAGAGAATGTCCGTGATTTAACCAGTAGTATTTCTCAAACCAAAGCGGACAGAGCCAGTGGACGCAAGGAACAGCAGGAACAAAGGCGAAAAACCATTGCGAAGCGTCGCTCTGAAATGGAACAGGTCAAACAGAAAAAACAGCCTGCTTCTTCTGTTCATGAAAGACCGACTATAAGACAAGAACAATATCATGATGAACAGACCTCAAAACAGTCTAATATTCAGACTTCATATAAGGAATCTCAACAAGCCAAACAAGAGCGTCCAGCAGTTAAGTCCGATTTTTCAAGTCCAAAAGTGGAACGCCAAGGCAATACCGTTCAAGAAAAAACCGTTCAAAAGCCAGCAACTTCAACCACTACAGCAGATAGAACTTCACAACGTCCAATCACAAAAGAACGTCCGTCTACTGTTCAAAGAGTACCACTACAAAATACAAGAAGTAGACCACCAATCAAAACCGCCACCATTAAGAAAGTCGGTAAGAAACCATGA", "aa": "MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQAAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNFIWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLLLLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDISNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRVESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFVFLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLIITTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRVMRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGKEKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRDLTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTIRQEQYHDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTTADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP", "aa_hexdigest": "fdb9a91ec5a8d1ad77011d8d73bd0ce1", "start_type": "GTG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_A9J076", "query_cov": 0.9972413793103448, "subject_cov": 0.9588859416445623, "identity": 0.9940000000000001, "score": 1303.0, "evalue": 0.0, "valid": true, "product": "YtxH domain-containing protein", "uniref50_id": "UniRef50_H1BBB6" }, "pscc": { "uniref50_id": "UniRef50_H1BBB6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_H1BBB6" ], "product": "YtxH domain-containing protein" }, "genes": [ "ytxH" ], "id": "GLJLFKCOHC_1064", "locus": "GCA013368035_01063" }, { "type": "cds", "sequence": "contig_1", "start": 616130, "stop": 617131, "strand": "+", "frame": 2, "gene": "nlpC", "product": "NlpC/P60 domain-containing protein", "db_xrefs": [ "COG:COG0791", "COG:M", "RefSeq:WP_000769868.1", "SO:0001217", "UniParc:UPI00000B51B9", "UniRef:UniRef100_A0A0G2PME6", "UniRef:UniRef50_Q2PJU3", "UniRef:UniRef90_R0N6K9" ], "nt": "ATGAAGTTGAAAACTTTAGTGATTGGTGGTTCTGGATTATTCTTGATGGTCTTCTCACTGCTTCTGTTTGTTGCCATTTTATTTTCAGATGAACAGGACAGCGGAATTTCCAATATTCATTATGGAGGTGTGAATGTTTCCGCAGAAGTGCTGGCTCATAAGCCTATGGTAGAAAAATATGCCAAAGAATATGGCGTTGAAGAATATGTCAACATACTTCTTGCGATTATACAGGTGGAATCGGGCGGTACTGCGGAAGATGTTATGCAGTCCTCGGAATCCCTCGGTCTTCCACCTAATTCATTGAGTACAGAAGAATCCATTAAGCAAGGTGTGAAGTATTTCAGTGAATTATTAGCCAGTAGCGAAAGGCTCAGTGTAGATTTAGAATCGGTTATCCAGTCCTACAATTATGGTGGTGGTTTCTTAGGGTATGTGGCTAATCGTGGAAATAAATATACCTTTGAACTGGCTCAAAGTTTCTCAAAAGAGTATTCAGGTGGCGAAAAAGTGTCTTACCCCAATCCCATAGCCATACCTATCAATGGGGGCTGGCGATACAACTATGGCAATATGTTTTATGTGCAACTGGTAACGCAGTATCTTGTCACAACAGAGTTTGATGATGATACGGTACAAGCCATCATGGACGAAGCACTGAAATATGAGGGCTGGCGATACGTTTACGGTGGAGCTTCCCCGACTACTTCTTTTGATTGTAGCGGACTGACACAATGGACGTATGGAAAAGCTGGAATTAACTTACCACGAACCGCACAACAGCAATATGATGTGACCCAGCATATCCCACTATCGGAAGCACAAGCTGGCGATTTGGTTTTCTTTCATTCTACCTATAACGCTGGCTCTTATATTACTCATGTTGGGATATACCTTGGCAATAACCGTATGTTTCATGCAGGCGACCCAATCGGTTATGCCGACTTAACAAGCCCCTACTGGCAACAGCATTTAGTGGGAGCAGGACGAATCAAACAATGA", "aa": "MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGVNVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSLSTEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQSFSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDEALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQAGDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ", "aa_hexdigest": "f1895a2693bf0e70b5f740c0d640b5ca", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00000B51B9", "ncbi_nrp_id": "WP_000769868.1", "uniref100_id": "UniRef100_A0A0G2PME6", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B51B9", "RefSeq:WP_000769868.1", "UniRef:UniRef100_A0A0G2PME6" ] }, "ips": { "uniref100_id": "UniRef100_A0A0G2PME6", "uniref90_id": "UniRef90_R0N6K9" }, "psc": { "uniref90_id": "UniRef90_R0N6K9", "gene": "NlpC", "product": "NlpC/P60 domain-containing protein", "uniref50_id": "UniRef50_Q2PJU3", "cog_id": "COG0791", "cog_category": "M" }, "pscc": { "uniref50_id": "UniRef50_Q2PJU3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q2PJU3" ], "product": "NlpC/P60 domain-containing protein" }, "genes": [ "nlpC" ], "id": "GLJLFKCOHC_1065", "locus": "GCA013368035_01064" }, { "type": "cds", "sequence": "contig_1", "start": 617128, "stop": 618063, "strand": "+", "frame": 1, "gene": "tcpC", "product": "Conjugative transposon protein TcpC", "db_xrefs": [ "RefSeq:WP_175949966.1", "SO:0001217", "UniParc:UPI00158BCC96", "UniRef:UniRef100_UPI00158BCC96", "UniRef:UniRef50_F7KH35", "UniRef:UniRef90_E4KMA0" ], "nt": "ATGAGAAAGGAAGATTTAATGATGAAATTTAGAAAAAATCAGAATAAAGAAAAACAGATACCAAAGGAAAAGAAACCTCGTGTCTACTATAAGGTCAATCCTCATAAAAAGGTTGTGATTGCCTTGTGGGTACTTTTAGGGCTTAGTTTCAGCTTTGCGATATTCAAGCACTTTACAGCTATAGATACTCATACTATTCACGAAACAACTATCATAGAAAAGGAATACGTTGATACTCATCATGTAGAAAATTTTGTAGAGAACTTTGCGAAAGTCTACTATTCATGGGAGCAATCCGATAAGTCCATTGATAATCGAATGGAAAGTCTAAAAGGCTATCTGACAGATGAACTTCAAGCTCTCAATGTTGATACAGTACGCAAAGATATTCCTGTATCGTCTTCTGTAAGAGGATTTCAGATATGGACGGTAGAGCCAACTGGCGACAATGAGTTTAATGTAACCTACAGTGTAGACCAGCTCATTACAGAGGGAGAAAATACAAAGACCGTCCACTCTGCTTATATAGTGAGTGTCTATGTAGATGGTTCTGGAAATATGGTACTGGTTAAGAATCCGACCATTACCAACATACCTAAGAAATCAAGTTATAAACCAAAAGCCATTGAAAGTGAGGGGACGGTTGATTCCATTACAACCAATGAAATCAATGAGTTTTTAACGACGTTCTTCAAGCTCTATCCTACAGCGACAGCCAGTGAACTTTCCTACTATGTGAATGACGGGATATTAAAACCAATCGGAAAAGAGTACATCTTTCAAGAACTGGTAAATCCTATTCACAATCGTAAGGATAATCAAGTCACGGTATCGCTGACAGTGGAGTATATCGACCAGCAGACCAAAGCAACGCAGGTATCTCAATTTGATTTGGTACTTGAAAAGAACAGGAGTAATTGGAAGATTATAGAATAA", "aa": "MRKEDLMMKFRKNQNKEKQIPKEKKPRVYYKVNPHKKVVIALWVLLGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSIDNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQLITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSITTNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTVSLTVEYIDQQTKATQVSQFDLVLEKNRSNWKIIE", "aa_hexdigest": "521a01c62b3c696e30c18902f98ccc9f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "ups": { "uniparc_id": "UPI00158BCC96", "ncbi_nrp_id": "WP_175949966.1", "uniref100_id": "UniRef100_UPI00158BCC96", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158BCC96", "RefSeq:WP_175949966.1", "UniRef:UniRef100_UPI00158BCC96" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158BCC96", "uniref90_id": "UniRef90_E4KMA0" }, "psc": { "uniref90_id": "UniRef90_E4KMA0", "product": "Conjugative transposon protein TcpC", "uniref50_id": "UniRef50_F7KH35" }, "pscc": { "uniref50_id": "UniRef50_F7KH35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F7KH35" ], "product": "Conjugative transposon protein" }, "genes": [ "tcpC" ], "id": "GLJLFKCOHC_1066", "locus": "GCA013368035_01065" }, { "type": "ncRNA", "class": null, "sequence": "contig_1", "start": 618220, "stop": 618384, "strand": "+", "gene": "sRNA_0030", "product": "Enterococcus sRNA 30", "score": 198.1, "evalue": 5.4e-28, "db_xrefs": [ "RFAM:RF02864", "SO:0000655" ], "nt": "GTCAATTTGATAGTGGGAACAAATAATTGGATGTCCTTTTTTAGGAGGGCTTAGTTTTTTGTACCCAGTTTAAGAATACCTTTATCATGTGATTCTAAAGTATCCAGAGAATATCTGTATGCTTTGTATACCTATGGTTATGCATAAAAATCCCAGTGATAAAAG", "id": "GLJLFKCOHC_41", "locus": "GCA013368035_01066" }, { "type": "sorf", "sequence": "contig_1", "start": 618338, "stop": 618424, "strand": "+", "gene": "tet(M)", "product": "tetracycline resistance ribosomal protection protein Tet(M)", "frame": 2, "db_xrefs": [ "COG:COG0480", "COG:J", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0032790", "GO:0046677", "KEGG:K18220", "RefSeq:WP_011058339.1", "SO:0001217", "UniParc:UPI00000E29F7", "UniRef:UniRef100_Q06SL5", "UniRef:UniRef50_Q47810", "UniRef:UniRef90_Q47810" ], "aa": "MLCIPMVMHKNPSDKSIYHWDFYALLGF", "aa_hexdigest": "e115f0495410ee952902df6e482318d4", "nt": "ATGCTTTGTATACCTATGGTTATGCATAAAAATCCCAGTGATAAAAGTATTTATCACTGGGATTTTTATGCCCTTTTGGGTTTTTGA", "ups": { "uniparc_id": "UPI00000E29F7", "ncbi_nrp_id": "WP_011058339.1", "uniref100_id": "UniRef100_Q06SL5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000E29F7", "RefSeq:WP_011058339.1", "UniRef:UniRef100_Q06SL5" ] }, "ips": { "uniref100_id": "UniRef100_Q06SL5", "uniref90_id": "UniRef90_Q47810" }, "psc": { "uniref90_id": "UniRef90_Q47810", "gene": "tet(M)", "product": "tetracycline resistance ribosomal protection protein Tet(M)", "uniref50_id": "UniRef50_Q47810", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K18220", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0032790", "GO:0046677" ] }, "pscc": { "uniref50_id": "UniRef50_Q47810", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q47810" ], "product": "Tetracycline resistance protein TetM from transposon TnFO1" }, "genes": [ "tet(M)" ], "id": "GLJLFKCOHC_1226", "locus": "GCA013368035_01067" }, { "type": "cds", "sequence": "contig_1", "start": 618440, "stop": 620359, "strand": "+", "frame": 2, "gene": "tet(M)", "product": "tetracycline resistance ribosomal protection protein Tet(M)", "db_xrefs": [ "COG:COG0480", "COG:J", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0032790", "GO:0046677", "KEGG:K18220", "NCBIProtein:WP_050232631.1", "RefSeq:WP_175950005.1", "SO:0001217", "UniParc:UPI00158B09BD", "UniRef:UniRef100_UPI00158B09BD", "UniRef:UniRef50_Q47810", "UniRef:UniRef90_Q47810" ], "nt": "ATGAAAATTATTAATATTGGAGTTTTAGCTCATGTTGATGCAGGAAAAACTACCTTAACAGAAAGCTTATTATATAACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCAAAATGGAATTGATTTATCAACGGTTTATCAGGATATTAAAGAGAAACTTTCTGCCGAAATTGTAATCAAACAGAAGGTAGAACTGTATCCTAATATGTGTGTGACGAACTTTACCGAATCTGAACAATGGGATACGGTAATAGAGGGAAACGATGACCTTTTAGAGAAATATATGTCCGGTAAATCATTAGAAGCATTGGAACTCGAACAAGAGGAGAGCATAAGATTTCAGAATTGTTCCCTGTTCCCTGTTTATCACGGAAGTGCAAAAAACAATATAGGGATTGATAACCTTATAGAAGTGATTACGAATAAATTTTATTCATCAACACATCGAGGTCAGTCTGAACTTTGCGGAAAAGTTTTCAAAATTGAGTATTCGGAAAAAAGACAGCGTCTTGCATATATACGTCTTTATAGTGGCGTACTGCATTTGCGAGATTCGGTTAGAATATCGGAAAAGGAAAAAATAAAAATTACAGAAATGTATACTTCAATAAATGGTGAATTATGTAAAATCGATAAGGCTTATTCTGGAGAAATTGTTATTTTGCAAAATGAGTTTTTGAAGTTAAATAGTGTTCTTGGAGATACAAAACTATTGCCACAGAGAAAAAAGATTGAAAATCCGCACCCTCTACTACAAACAACTGTTGAACCGAGTAAACCTGAACAGAGAGAAATGTTGCTTGATGCCCTTTTGGAAATCTCAGATAGTGATCCGCTTCTACGATATTACGTGGATTCTACGACACATGAAATTATACTTTCTTTCTTAGGGAAAGTACAAATGGAAGTGATTAGTGCACTGTTGCAAGAAAAGTATCATGTGGAGATAGAACTAAAAGAGCCTACAGTCATTTATATGGAGAGACCGTTAAAAAATGCAGAATATACCATTCACATCGAAGTGCCGCCAAATCCTTTCTGGGCTTCCATTGGTTTATCTGTATCACCGCTTCCGTTGGGAAGTGGAATGCAGTATGAGAGCTCGGTTTCTCTTGGATACTTAAATCAATCATTTCAAAATGCAGTTATGGAAGGGATACGCTATGGTTGCGAACAAGGATTATATGGTTGGAATGTGACGGACTGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAGAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTTACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCATTCTTAGTGGAGAAATCCCTGCTCGGTGTATTCAAGAATATCGTAGTGATTTAACTTTCTTTACAAATGGACGTAGTGTTTGTTTAACAGAGTTAAAAGGGTACCATGTTACTACCGGTGAACCTGTTTGCCAGCCCCGTCGTCCAAATAGTCGGATAGATAAAGTACGATATATGTTCAATAAAATAACTTAG", "aa": "MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPVYHGSAKNNIGIDNLIEVITNKFYSSTHRGQSELCGKVFKIEYSEKRQRLAYIRLYSGVLHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLLRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVRYMFNKIT", "aa_hexdigest": "4d115ce453bfce956f6fbdac8dec17b3", "start_type": "ATG", "rbs_motif": "GGAGG", "ups": { "uniparc_id": "UPI00158B09BD", "ncbi_nrp_id": "WP_175950005.1", "uniref100_id": "UniRef100_UPI00158B09BD", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158B09BD", "RefSeq:WP_175950005.1", "UniRef:UniRef100_UPI00158B09BD" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158B09BD", "uniref90_id": "UniRef90_Q47810" }, "psc": { "uniref90_id": "UniRef90_Q47810", "gene": "tet(M)", "product": "tetracycline resistance ribosomal protection protein Tet(M)", "uniref50_id": "UniRef50_Q47810", "cog_id": "COG0480", "cog_category": "J", "kegg_orthology_id": "K18220", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0032790", "GO:0046677" ] }, "pscc": { "uniref50_id": "UniRef50_Q47810", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q47810" ], "product": "Tetracycline resistance protein TetM from transposon TnFO1" }, "expert": [ { "type": "amrfinder", "rank": 95, "gene": "tet(M)", "product": "tetracycline resistance ribosomal protection protein Tet(M)", "method": "BLASTP", "query_cov": 1.0, "model_cov": 1.0, "identity": 0.9969, "id": "WP_050232631.1", "db_xrefs": [ "NCBIProtein:WP_050232631.1" ] } ], "genes": [ "tet(M)" ], "id": "GLJLFKCOHC_1067", "locus": "GCA013368035_01068" }, { "type": "cds", "sequence": "contig_1", "start": 620371, "stop": 620991, "strand": "+", "frame": 1, "gene": null, "product": "Recombinase family protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D1X8Q7", "UniRef:UniRef90_UPI0019398931" ], "nt": "TTGTTATATAAATGTGGCTTCTTGTGTAAGCAAATTAGAGAAGAATTGTTAGATGATGCTGTTGCAGAGGTAATTGTCAAGATTGTAAGTAATCCGAAATTTGCTTCTATGATGCAAGAGAAAATCAACATGAAGGTGGATACCTCTGAAATAGAAACAGAAATAGATAATTACCAAAAGGAATTGAGGAAGTGTCATTCTACAAAGTTTAAGCTAATTGAGGAAATAGATAATTTAGATGTTGAAGATAAGCACTATAAGAGAAGGAAACAGGATTTAGACGACAGACTTTATCGCATGTATGACAAAATAGATGAATTAGAATCATCACTAATTGATGCGAAAGCAAAGAAACAGACTATTGAAGCTGAAAAGCTTACAGGAGATAACATATATAAGGTTCTGATCTATTTTGATAAGCTCTATAAAGTCATGAATGATGTAGAGCGTAGACAGTTAATTACAGCTTTGATTTCTGAAATTCAAGTTTATGAAGAAAAACAGCCTAACGGACAATGGCTAAAATCAATTACTTTCAAGCTTCCGATTATAGAAGAAAATCTCAACATAGGTTTGGACAATGATGAGCACGTTGAAATGTTGTCACTTTTGAAAAAATAA", "aa": "MLYKCGFLCKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTSEIETEIDNYQKELRKCHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLITALISEIQVYEEKQPNGQWLKSITFKLPIIEENLNIGLDNDEHVEMLSLLKK", "aa_hexdigest": "b883885cc5bfb49547e7e35a19ba7ea5", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0019398931", "query_cov": 0.9271844660194175, "subject_cov": 1.0, "identity": 0.995, "score": 365.0, "evalue": 1.72e-127, "valid": true, "uniref50_id": "UniRef50_A0A3D1X8Q7" }, "pscc": { "uniref50_id": "UniRef50_A0A3D1X8Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D1X8Q7" ], "product": "Recombinase family protein" }, "genes": [], "id": "GLJLFKCOHC_1068", "locus": "GCA013368035_01069" }, { "type": "cds", "sequence": "contig_1", "start": 621241, "stop": 621537, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGCTTTGGGTTATGTATCAAGCGCAATTGTTGCAAACGAAATTGGAAAACAAAATTTTGAAGCTGCTCATGAACTCGGAAATAGTGCATTAAAGTTTGGCTTTTATGCACAGTCATTATTTTCAATATTAGGAGTTTCATTAACCTGGCCTTTGCTAAGACTTTATTCAATAGATATTCAAATAATTAATTCTGTTGGGTATGGTGTTATGGGAATTATGATGCTAAGAGCTATTCTGGATATAGGAAACCTTACAACCCTAAGGGCTTTATGGGGCGCCAATGATGTTTGA", "aa": "MIALGYVSSAIVANEIGKQNFEAAHELGNSALKFGFYAQSLFSILGVSLTWPLLRLYSIDIQIINSVGYGVMGIMMLRAILDIGNLTTLRALWGANDV", "aa_hexdigest": "a0f7b2932e5f795e14d6b83c3ad40064", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10622.31879999999, "isoelectric_point": 5.564105415344237 }, "id": "GLJLFKCOHC_1069", "locus": "GCA013368035_01070" }, { "type": "cds", "sequence": "contig_1", "start": 621655, "stop": 621831, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTGCACTTGAACCAATTTTGGGGGCAATATTTCATTTAACAATTGATTATTCAATTTCCTATCACTTTATAACAAAAAACAATAAAATTTCAATTAAAGAAACATGGAATACAAAATATGTTAAAAACATTCTTAAGTTAGGTCTTCCCATTGCTTTTGAAACAGGAATTTGA", "aa": "MVALEPILGAIFHLTIDYSISYHFITKNNKISIKETWNTKYVKNILKLGLPIAFETGI", "aa_hexdigest": "7acff5c6a09afb8e54dda36c12eae887", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6622.767399999999, "isoelectric_point": 9.101533699035645 }, "id": "GLJLFKCOHC_1070", "locus": "GCA013368035_01071" }, { "type": "cds", "sequence": "contig_1", "start": 621886, "stop": 622170, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAAAAATCACAACATCACTATAAATGATTTAGCTAGAATGAATCAATTTAATAAGGTGCTATACGGTGGAACTATAAATGGAATTACCCTAGTTAATACAAATCCTTTTAAGGTTTCAGTAACTTCCGAAACAACAATGGCTATAAAATTCTTAAGAATAACCACTTGCGACATATTCATATATTCAATAGCCGTTATTTTTACGGCCAGTTTACAATCTGTTGAAAAAAATAAATTTTCAATATTTGGATTAATTACCAGTATATTTGTACGTGCAAGTTGA", "aa": "MKNHNITINDLARMNQFNKVLYGGTINGITLVNTNPFKVSVTSETTMAIKFLRITTCDIFIYSIAVIFTASLQSVEKNKFSIFGLITSIFVRAS", "aa_hexdigest": "723e38f4e0c9e5227a8e43db31948215", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10498.180399999992, "isoelectric_point": 9.80527057647705 }, "id": "GLJLFKCOHC_1071", "locus": "GCA013368035_01072" }, { "type": "cds", "sequence": "contig_1", "start": 622216, "stop": 622695, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGAAAGAAAGAAAACAGTAAACAAAGAAATACAAATCGTTTAAGTATTTTTATTGAAAAATCAAGTGAATTATTTAAAGTTAAACACTTTAAGTATATTTTCAAATTGACTTTACCAATCTTTTTTCAGACACTTTTTGTTGCTTTAGTATCGGTAGTTTCTTCTCTTGCTTCTTCGCATTATATAAAAGTCTATCACGCCGATGGTTCATACAATGGTTTTTATTTTTATATGATTGCAAAAATATTAACTGTTTACAAAATGATAACCTTTATTCCTATTATTTACCAAAGCGGAACATTAGTCTTATGCTCAAATCTTTTTGGACAAAGCAAAATAAGAGACATACCAAAAGCTTTGTGGTCTGCCGTTTATGTATCATTAATAATAAATACTTGTGTCTATTTTATTATGTTTGGACTTGCACCAATAGTTTTACAAGCTTCTGGAACAAAAAACATTCCTCTTTTTGGTTGA", "aa": "MRKKENSKQRNTNRLSIFIEKSSELFKVKHFKYIFKLTLPIFFQTLFVALVSVVSSLASSHYIKVYHADGSYNGFYFYMIAKILTVYKMITFIPIIYQSGTLVLCSNLFGQSKIRDIPKALWSAVYVSLIINTCVYFIMFGLAPIVLQASGTKNIPLFG", "aa_hexdigest": "db464adc4046dff1ab82c559d7447749", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18207.470799999992, "isoelectric_point": 9.869481086730957 }, "id": "GLJLFKCOHC_1072", "locus": "GCA013368035_01073" }, { "type": "cds", "sequence": "contig_1", "start": 622987, "stop": 623379, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00448.28", "PFAM:PF02881.24" ], "nt": "TTGCAAAACAAGCATAATAAAATTGACGAAGAATTTTTTGAAGATTTAGAAGAAATGCTGATTATGTCTGATATTTCAATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAACGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGATAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATAATAGGTTGAATGTAATATTAGTTGTGGGTGTCAATGGTTCAGGAAAAACTACGTCAATATCAAAAATTGCTAAAAAATTAATTGACGAGGGTAAAAAAGTATTGATTGCTGCAGGTGATACATTTAGAGCAGCGGCAGTTGAACAATTAGAGATTTGA", "aa": "MQNKHNKIDEEFFEDLEEMLIMSDISIKLVQIIINECKKEVRNENITDPKLINEIIADKLFTIYTSNSVVDTTLNIKDNRLNVILVVGVNGSGKTTSISKIAKKLIDEGKKVLIAAGDTFRAAAVEQLEI", "aa_hexdigest": "32b482f41e01095d3ff1d7245a548d38", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00448.28", "name": "SRP54", "length": 48, "aa_cov": 0.36923076923076925, "hmm_cov": 1.0, "evalue": 1.5736087461481633e-20, "score": 69.00234985351562, "start": 82, "stop": 129 }, { "id": "PF02881.24", "name": "SRP54_N", "length": 62, "aa_cov": 0.43846153846153846, "hmm_cov": 1.0, "evalue": 4.933775696880977e-15, "score": 50.97073745727539, "start": 4, "stop": 60 } ], "seq_stats": { "molecular_weight": 14575.721299999977, "isoelectric_point": 5.072220802307128 }, "id": "GLJLFKCOHC_1073", "locus": "GCA013368035_01074" }, { "type": "cds", "sequence": "contig_1", "start": 623425, "stop": 623850, "strand": "+", "frame": 1, "gene": "ftsY", "product": "Signal recognition particle-docking protein FtsY", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "nt": "GTGGATCCGGCGGCGGTTGTTTATAGGGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCGGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATCAATAGGGTTTTAGCCTCTAAAATTCCCGATGCACCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCTAGGGTATTCAAGGAATCTACACCATTGACTGGAATCATTCTAACAAAAATGGATGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGTGAAAAAGTTGATGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTGACAAAAGGTATTAACGAATAA", "aa": "MDPAAVVYRAIDIAKSKKYDILIVDTAGRLQNKVNLMNELAKINRVLASKIPDAPHESLLVLDATTGQNGVIQARVFKESTPLTGIILTKMDGTSKGGIVLTIKDEIGLFVKYIGLGEKVDDLAEFDLDSYIYGLTKGINE", "aa_hexdigest": "6681501c031acd94ed94cda22d44d58a", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A449AH26", "query_cov": 0.9361702127659575, "subject_cov": 0.8407643312101911, "identity": 0.705, "score": 191.0, "evalue": 2.92e-60, "valid": false, "product": "Signal recognition particle-docking protein FtsY", "uniref50_id": "UniRef50_A0A449AH26" }, "pscc": { "uniref50_id": "UniRef50_A0A449AH26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449AH26" ], "product": "Signal recognition particle-docking protein FtsY" }, "genes": [ "ftsY" ], "id": "GLJLFKCOHC_1074", "locus": "GCA013368035_01075" }, { "type": "cds", "sequence": "contig_1", "start": 623850, "stop": 624074, "strand": "+", "frame": 3, "gene": "ylxM", "product": "putative DNA-binding protein YlxM, UPF0122 family", "db_xrefs": [ "COG:COG2739", "COG:K", "KEGG:K09787", "RefSeq:WP_020002601.1", "SO:0001217", "UniParc:UPI00031E45B5", "UniRef:UniRef100_A0A2K9YTY8", "UniRef:UniRef50_C4XE46", "UniRef:UniRef90_A0A2K9YTY8" ], "nt": "ATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTTTATATGAAGATTTGTCATATGGGGAATTAGCTCAAGAATTAGCAATGTCTAGACCAGGGGCTTACGACGCAGTTAAAAAAGCAAAATTACACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAA", "aa": "METIEERDYYVKLFEKYGDLLPQSQKQALQLLLYEDLSYGELAQELAMSRPGAYDAVKKAKLHLKQIDEKLKQS", "aa_hexdigest": "78c4178b8a69d62122a90a1d2b675034", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI00031E45B5", "ncbi_nrp_id": "WP_020002601.1", "uniref100_id": "UniRef100_A0A2K9YTY8", "db_xrefs": [ "SO:0001217", "UniParc:UPI00031E45B5", "RefSeq:WP_020002601.1", "UniRef:UniRef100_A0A2K9YTY8" ] }, "ips": { "uniref100_id": "UniRef100_A0A2K9YTY8", "uniref90_id": "UniRef90_A0A2K9YTY8" }, "psc": { "uniref90_id": "UniRef90_A0A2K9YTY8", "gene": "YlxM", "product": "Predicted DNA-binding protein YlxM, UPF0122 family", "uniref50_id": "UniRef50_C4XE46", "cog_id": "COG2739", "cog_category": "K", "kegg_orthology_id": "K09787" }, "pscc": { "uniref50_id": "UniRef50_C4XE46", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_C4XE46" ], "product": "Sigma-70, region 4" }, "genes": [ "ylxM" ], "id": "GLJLFKCOHC_1075", "locus": "GCA013368035_01076" }, { "type": "cds", "sequence": "contig_1", "start": 624173, "stop": 624340, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGTTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTAGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTTGCGATTGTTGTCGGTGGCGGTAATTTTTGA", "aa": "MSYKRVLIKLSGEGLANKGKHLAIDYELVEKFANQLKTIISRNIEVAIVVGGGNF", "aa_hexdigest": "6d8fef6658cc5c79d62692c6239603c9", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6047.0342, "isoelectric_point": 9.52193202972412 }, "id": "GLJLFKCOHC_1076", "locus": "GCA013368035_01077" }, { "type": "cds", "sequence": "contig_1", "start": 624368, "stop": 624901, "strand": "+", "frame": 2, "gene": null, "product": "UMP kinase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "nt": "ATGCAAAGGGTTAGAGCAGATTACATTGGTATGTTAGCTACAACAATGAACGCGCTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCAAGCCTAACAATGGACCCAAAGGTTTGTGAAGTTTATATAAACGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTAATTTTTGCAGGAGGAACTGGACGTCCATTTTTTACAACCGATACAGCCTCAACATTATTCGCTAGTGAAATTGATGCGGATGCAATATTGATGGGAAAAAACAACGTTGATGGGGTGTATGATAGCGATCCAAAATTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTACTAGAAAAGGAATTGAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAGGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAA", "aa": "MQRVRADYIGMLATTMNALALQSGFERLNLHCRVLSSLTMDPKVCEVYINEKAKKYLRDGEVVIFAGGTGRPFFTTDTASTLFASEIDADAILMGKNNVDGVYDSDPKFNKDAKKFPIITYDELLEKELKVIDQTAASMAKENNIDILIFDINEEDSLIRAIENKIPNTIITREREK", "aa_hexdigest": "9d37546a13ad5001476268f5f5e1a856", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A3B0P6F8", "query_cov": 0.96045197740113, "subject_cov": 1.0, "identity": 0.841, "score": 286.0, "evalue": 6.43e-97, "valid": false, "product": "UMP kinase", "ec_ids": [ "2.7.4.22" ], "uniref50_id": "UniRef50_A0A3B0P6F8", "kegg_orthology_id": "K09903" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0P6F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0P6F8" ], "product": "UMP kinase" }, "genes": [], "id": "GLJLFKCOHC_1077", "locus": "GCA013368035_01078" }, { "type": "cds", "sequence": "contig_1", "start": 624901, "stop": 625452, "strand": "+", "frame": 1, "gene": "frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "db_xrefs": [ "COG:COG0233", "COG:J", "RefSeq:WP_044329746.1", "SO:0001217", "UniParc:UPI0005C83D5E", "UniRef:UniRef100_UPI0005C83D5E", "UniRef:UniRef50_B3PLW1", "UniRef:UniRef90_D1J8P9" ], "nt": "ATGGAATTAGACAGTTATCTTTTAGAACTAGATGAAGAAATTAGCAAATCAATTACTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGGGCAAATCCTGTCTTAATCAGTAAAATTAAATTAGAATATTATGGTTCACCAATTTCGGTAGAAGAAATAGCATCTATAACTACATCAGGACCATTACAATTAATTGTTAAGCCATATGATATGAGTGTGCTAAAAAATATTGAAAAAGCAATCATGGATAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATCAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTAACAATATAA", "aa": "MELDSYLLELDEEISKSITNYENQLMKVVIGRANPVLISKIKLEYYGSPISVEEIASITTSGPLQLIVKPYDMSVLKNIEKAIMDNKINVSIINEGHQLRLQYPQMTTEKRVEMTKQLASITEQARIGVRQARQDINKKIKADSDLSEDIQKHYLDNIQKEVDKSIEKINEMSKTKEKELLTI", "aa_hexdigest": "55a096ccefd76ab8d64c2c5e0c6bd8a0", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005C83D5E", "ncbi_nrp_id": "WP_044329746.1", "uniref100_id": "UniRef100_UPI0005C83D5E", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C83D5E", "RefSeq:WP_044329746.1", "UniRef:UniRef100_UPI0005C83D5E" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C83D5E", "uniref90_id": "UniRef90_D1J8P9" }, "psc": { "uniref90_id": "UniRef90_D1J8P9", "gene": "Frr", "product": "Ribosome recycling factor (Ribosome releasingfactor)(RRF)", "uniref50_id": "UniRef50_B3PLW1", "cog_id": "COG0233", "cog_category": "J" }, "pscc": { "uniref50_id": "UniRef50_B3PLW1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLW1" ], "product": "Ribosome recycling factor" }, "genes": [ "frr" ], "id": "GLJLFKCOHC_1078", "locus": "GCA013368035_01079" }, { "type": "cds", "sequence": "contig_1", "start": 626221, "stop": 626463, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATTTCGGAAACTCTGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTACGGCCGTTTTTGTTCTTCCTGCAATTTCTATTATTGGCGATTTAGTATTTTCAGGAATAAAAAGAGCTCAAAATATTAAGGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGATAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAA", "aa": "MGINFGNSDSIKILINQKQIITAVFVLPAISIIGDLVFSGIKRAQNIKDFSELIAGHGGIMDRFDSLSFNTVFLTFILMI", "aa_hexdigest": "3abe1bc2c0a1005faea91ebe37609cbc", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8790.275099999995, "isoelectric_point": 6.520194053649903 }, "id": "GLJLFKCOHC_1079", "locus": "GCA013368035_01080" }, { "type": "cds", "sequence": "contig_1", "start": 626566, "stop": 627087, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAACAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAACCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTAAAATATAATTATTCATGTATAACTACTTCGGCAATTGAAATAGATGAAAAAACATTAACACTTTCCTTACTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGATTAAACAATAAAAAAAGCGTTGCAAGCAAAAAACTAGACGGTTGA", "aa": "MLDKTFVQLCKEIKFEFDEQQFYGAKVLSVESNLNEWKFKIAFANHIDIEQYKQFREKLVKRFGKVELILAIETIIRNKQIIVDYLNYLIECNPKKYWPFVKYNYSCITTSAIEIDEKTLTLSLLTNEIYEVFKSNKDLFKEALASIGFDFYNVVFNLGLNNKKSVASKKLDG", "aa_hexdigest": "1fb54a7fb45785f11e72ef2a585caf50", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 20372.389500000005, "isoelectric_point": 8.278788185119634 }, "id": "GLJLFKCOHC_1080", "locus": "GCA013368035_01081" }, { "type": "cds", "sequence": "contig_1", "start": 627190, "stop": 628260, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF13482.12", "PFAM:PF00929.31" ], "nt": "ATGGACTTAACTACTGCAAACGAAACGTTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTTATTGAAAGCAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTATTAAAAAATTTTATGATTCTGAAAACGAAATACCAGATATAAAGGTAGGGGACACGATTATTGTTACTGGAAAATTGAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAACTTATTGAAGATAACGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAATATGAGTGCTCAAGATGGAATTTCAACACCATCTGATTATATTGATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCTGAATTTTATAATTATGCCAAAAAGCAAAAGGATGTTAAGCCAATATACGGCGCAACATTAAGCACTATTGATTCAGATAAAAAATTCTTCTACAATTTTAAAAATTTCAACTTAAAAGAAGAAGAATATGTTGTGTTTGACCTTGAAACAACAGGATTGAGCCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTACAAACAAAACGTGAGCTATCAGCCTTTACTGTTGAAAGCAGTCACATTAATAATGAAATGTTAAAGAATGGTTATGAACAAGAAGAAGGCATTAAAAAAATTTATGAAATATTAAAGGATAAAATAGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTGGCAAACTCGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTGGCCTTATCTTATTTTTTAAACCCCGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTATTTATGATACAACTGTTGCCCACCGTGGTGATTACGACGCTAATGTTTTGGCTAAGATTTGA", "aa": "MDLTTANETFEEIKITTKGEVFFIESKVLKNKKILYTLKISDYKEAILIKKFYDSENEIPDIKVGDTIIVTGKLSDDNFTKSKVLIAMGKECFIKTEPLQKLIEDNEDNKRVELAARSNMSAQDGISTPSDYIDAAKFYGHKAIALTDLDDAQGFPEFYNYAKKQKDVKPIYGATLSTIDSDKKFFYNFKNFNLKEEEYVVFDLETTGLSPRLDDIIEFGATIIKNNTITQKIQFFLQTKRELSAFTVESSHINNEMLKNGYEQEEGIKKIYEILKDKIAVAHNANFDINFCKEKFGKLGLDNSKIFGLDTLALSYFLNPDEFRHSLGYVAKRFNIIYDTTVAHRGDYDANVLAKI", "aa_hexdigest": "7516cbdbadf91a63658870db2a03e310", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF13482.12", "name": "RNase_H_2", "length": 143, "aa_cov": 0.3960674157303371, "hmm_cov": 0.8321678321678322, "evalue": 1.6866866904897767e-08, "score": 30.000991821289062, "start": 201, "stop": 341 }, { "id": "PF00929.31", "name": "RNase_T", "length": 165, "aa_cov": 0.4410112359550562, "hmm_cov": 0.9878787878787879, "evalue": 1.2943140181665026e-31, "score": 105.76761627197266, "start": 200, "stop": 356 } ], "seq_stats": { "molecular_weight": 40711.82989999999, "isoelectric_point": 5.401034355163574 }, "id": "GLJLFKCOHC_1081", "locus": "GCA013368035_01082" }, { "type": "cds", "sequence": "contig_1", "start": 628267, "stop": 628530, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGATTGACAAACTTGCAAAAGATTACAATATCCTAAACTCACAGGATTTAAACAATGTCGATAGCCAAAAAATACTTTCTCATAAATTTACATATGAAACAAGAATATTGGTAAAAAATCAAGCCGGACTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCTCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGCACTGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGA", "aa": "MIDKLAKDYNILNSQDLNNVDSQKILSHKFTYETRILVKNQAGLKKLFKIISRSLTSQFKKNPQIYFDQLGTDEDLFFGSATNQSYV", "aa_hexdigest": "e105362f5a6e444f6efc89fb1221d240", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10080.355899999991, "isoelectric_point": 9.222669792175292 }, "id": "GLJLFKCOHC_1082", "locus": "GCA013368035_01083" }, { "type": "cds", "sequence": "contig_1", "start": 628561, "stop": 629139, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTAAATTCACTAAAATATTATGATTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGTAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCAGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAGATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGATAACTCGCCTCAAAAATTAAGAGAAGTTGTTTATAAGACTTTAAAAGAAAGATATGGGGATAATCCAGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAATCCAATTATAAAGTATGGCTATTCTGTTATTTATTGA", "aa": "MLNSLKYYDYIELPPISSFQYMIDSNHVTLDELKFLYKDLISKAKSLNKMCVAVSDCRYIYNYQRLIHEIYIEAPTLGGGLHWLKKRPSHPEFRYLTTREMLKEFAFLNDSYLINEIVIENPNKIANEISSNIQVIKDKLYVPTFDNSPQKLREVVYKTLKERYGDNPDPIIVKRIEKELNPIIKYGYSVIY", "aa_hexdigest": "af17b026e1be59bc442347efd93abd78", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 22730.060100000017, "isoelectric_point": 8.321143913269044 }, "id": "GLJLFKCOHC_1083", "locus": "GCA013368035_01084" }, { "type": "cds", "sequence": "contig_1", "start": 629332, "stop": 629610, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D", "PFAM:PF07733.18" ], "nt": "TTGCCAGACAAGCATTGTCCAAATTGCAACTCAATATTGTCAAAAGATGGGCATAATATCCCTTTTGAAACATTCTTAGGATTTGAAGCGGATAAAGTTCCTGATATTGATTTGAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAAGAATTGTTTGGCGAAGCACATACATATAGAGCTGGTACAATAGCAACTGTAGCAGAAAAAACAGCATATGGATTTTGCAAAAAATATGAAGAAGAAAAGAGAGGAAATTATCAACCTTGA", "aa": "MPDKHCPNCNSILSKDGHNIPFETFLGFEADKVPDIDLNFSGEYQPTIHNYVKELFGEAHTYRAGTIATVAEKTAYGFCKKYEEEKRGNYQP", "aa_hexdigest": "e91ce885ba96cabbf188544f2aa38639", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI00311D31FE", "query_cov": 0.8695652173913043, "subject_cov": 0.9523809523809523, "identity": 0.688, "score": 123.0, "evalue": 5.56e-35, "valid": false, "uniref50_id": "UniRef50_UPI0023B0427D" }, "pscc": { "uniref50_id": "UniRef50_UPI0023B0427D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0023B0427D" ] }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 59, "aa_cov": 0.6413043478260869, "hmm_cov": 1.0, "evalue": 1.2247975892881493e-19, "score": 66.29480743408203, "start": 18, "stop": 76 } ], "seq_stats": { "molecular_weight": 10477.550399999993, "isoelectric_point": 5.471401023864746 }, "id": "GLJLFKCOHC_1084", "locus": "GCA013368035_01085" }, { "type": "cds", "sequence": "contig_1", "start": 629617, "stop": 629766, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF07733.18" ], "nt": "TTGTTCTTGGATTTTTTAGCATCTAAAACACAAGGAGTAAAGAGGACAACTGGACAACACCCAGGAGGAATTATTATTATTCCTAAAGAATTCGATGTAGAAGATTTTACGCCTGTAAACTATCCTGCCAACGACACAGAAAGTCCTTGA", "aa": "MFLDFLASKTQGVKRTTGQHPGGIIIIPKEFDVEDFTPVNYPANDTESP", "aa_hexdigest": "013ec568ffe5429f053f3ee9f906ae61", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF07733.18", "name": "DNA_pol3_alpha", "length": 40, "aa_cov": 0.8163265306122449, "hmm_cov": 0.95, "evalue": 2.1594972418674102e-09, "score": 32.711971282958984, "start": 6, "stop": 45 } ], "seq_stats": { "molecular_weight": 5423.026500000003, "isoelectric_point": 4.646496772766113 }, "id": "GLJLFKCOHC_1085", "locus": "GCA013368035_01086" }, { "type": "cds", "sequence": "contig_1", "start": 629821, "stop": 630102, "strand": "+", "frame": 1, "gene": null, "product": "DNA polymerase III polC-type", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "nt": "TTGTTGGGTCATGATGATCCTACCGTTATTAGAATGCTTGAAACATTGACAAATACAAACGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTCTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGAGGCGAAACAACTGGAGCTTATGGGTTGCCTGAATTTGGAACAAACTTTGTAAGAAAAATGTTAAAAAAAGCTCAACCAAGATCATTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGA", "aa": "MLGHDDPTVIRMLETLTNTNAKEIPKFDAEVMKLFYTTESLKISPSDIGGETTGAYGLPEFGTNFVRKMLKKAQPRSFNDLILLSGLSHGTDV", "aa_hexdigest": "2d0c3d1ead0a0adca86ab0ead46ba3d3", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A432FBH3", "query_cov": 1.0, "subject_cov": 0.9117647058823529, "identity": 0.624, "score": 118.0, "evalue": 6.6e-33, "valid": false, "product": "DNA polymerase III alpha subunit finger domain-containing protein", "uniref50_id": "UniRef50_A0A3B0PG80" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PG80", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PG80" ], "product": "DNA polymerase III polC-type" }, "genes": [], "id": "GLJLFKCOHC_1086", "locus": "GCA013368035_01087" }, { "type": "cds", "sequence": "contig_1", "start": 630466, "stop": 630654, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAGTAGTGGCGTAGCAAGCGTTCACCAAAAACTTATTGAATTAAAAAATCGCCAAAATTCAAATGGTGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATATGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCTTTGGCTGATAAATGA", "aa": "MSSGVASVHQKLIELKNRQNSNGEKLSVKEEALIPVLEITQELYARGFKIKNVDLKLSLADK", "aa_hexdigest": "d0844758ac60e974c3ce09e4c0f782db", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6922.9564, "isoelectric_point": 9.09998645782471 }, "id": "GLJLFKCOHC_1087", "locus": "GCA013368035_01088" }, { "type": "cds", "sequence": "contig_1", "start": 630700, "stop": 630879, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF14579.12" ], "nt": "GTGGATGGTTTAGGAATTACTGTTGCTCAAACAATAGTATCTGCAAGAAAAGAAGGCTTTTTCCTTTCAATTGAAGACCTTGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCGGAATTAAAAAAAATAGGCGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAA", "aa": "MDGLGITVAQTIVSARKEGFFLSIEDLASRTKVNSRIISELKKIGVLDCLDDTNQVSLF", "aa_hexdigest": "9f00ef4ff60f338034dae1f1abda16da", "start_type": "GTG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF14579.12", "name": "HHH_6", "length": 56, "aa_cov": 0.8813559322033898, "hmm_cov": 1.0, "evalue": 6.039367681177621e-11, "score": 37.870018005371094, "start": 2, "stop": 53 } ], "seq_stats": { "molecular_weight": 6457.3866, "isoelectric_point": 5.086430549621581 }, "id": "GLJLFKCOHC_1088", "locus": "GCA013368035_01089" }, { "type": "cds", "sequence": "contig_1", "start": 631543, "stop": 631938, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTATTAATATTATCGGAATTGCAAGGAATACCAAACCTACAGCGGGTAAAACCCTAATATATCAATCTTCTGGTCTTTGGCCACTAGATACCAAAAATGAATTAATGGCTAAAGAAATTGGAATTAGAAAGGTTAATGCTTCAGTGTTAAATACCTTAAATTTAAAAACTAACAATAAAACAATAATAATTTGTATTGCAGCAATTGCTAATGCTATTGGAAACTCAACACCTCATCTTGATAAATTTTTTGTTGATTTTATAATTCAACCATCTATGCCTAAAATTAAAAACATTATAAAAGAAGGAATGACAATTAAAAATAAACCCGTTAGAGCTATTTTTCATGAACGTGATAATTCTCTTTTTGGCTTTCCTGATTCTACAACATAA", "aa": "MFINIIGIARNTKPTAGKTLIYQSSGLWPLDTKNELMAKEIGIRKVNASVLNTLNLKTNNKTIIICIAAIANAIGNSTPHLDKFFVDFIIQPSMPKIKNIIKEGMTIKNKPVRAIFHERDNSLFGFPDSTT", "aa_hexdigest": "695fa95a71adc7f3cf9619421060a697", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14540.933399999978, "isoelectric_point": 9.885920524597168 }, "id": "GLJLFKCOHC_1089", "locus": "GCA013368035_01090" }, { "type": "cds", "sequence": "contig_1", "start": 632061, "stop": 633317, "strand": "+", "frame": 3, "gene": "glyA", "product": "Serine hydroxymethyltransferase", "db_xrefs": [ "COG:COG0112", "COG:E", "EC:2.1.2.1", "KEGG:K00600", "RefSeq:WP_044420793.1", "SO:0001217", "UniParc:UPI0005C81B46", "UniRef:UniRef100_UPI0005C81B46", "UniRef:UniRef50_P56089", "UniRef:UniRef90_D1J8Q3" ], "nt": "ATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGACATTTATCACACGGATATAAAATTTCATTTAGTGGTAAATTTTACGAAGCACATACCTATTCTGTTAACGATCAAGGTTTATTGGATTATGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGCGATGAAGTAGGTGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCACCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATCTTTAAAAGAGTTAATAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGCTTTAGAGAAGCATTACAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAACTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAACACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTTTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACCAATTCATTTCCAATTAAACGTTCTCATTTAGTTCGTTAG", "aa": "MYKKISLDDKEIEELINLENQRLEDHVELIASENYVSEDVMKANGSCLTNKYAEGYPNKRYYGGCEYVDKIEEIAQERAKKLFNVKYANVQPYSGSVANAAVYMAMVDPGDKVLGLDLNSGGHLSHGYKISFSGKFYEAHTYSVNDQGLLDYDKILEIAKEVKPKMIICGYSAYSQIVDFAKFRKICDEVGAKLFADISHISGLIIAGLHPSPSGYADVIMTTTHKTLRGTRGAIILTNDEEIFKRVNSAVFPGVQGGPLLHQIAAKAVSFREALQPEFIDYQKQLLLNSKIMCQTFLNKGVKIISGLTQNHLFMIDVKTSYGITGKYATEVLGRVNITVNKNTIPNDSEKPTVASGIRIGSAAMTSRGLKEDEFIILANLIDKVLRDPENENLLYVVKKEIAKLTNSFPIKRSHLVR", "aa_hexdigest": "b8c5082caf53703babeccbbbd683622a", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005C81B46", "ncbi_nrp_id": "WP_044420793.1", "uniref100_id": "UniRef100_UPI0005C81B46", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C81B46", "RefSeq:WP_044420793.1", "UniRef:UniRef100_UPI0005C81B46" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C81B46", "uniref90_id": "UniRef90_D1J8Q3" }, "psc": { "uniref90_id": "UniRef90_D1J8Q3", "gene": "GlyA", "product": "Serine hydroxymethyltransferase", "ec_ids": [ "2.1.2.1" ], "uniref50_id": "UniRef50_P56089", "cog_id": "COG0112", "cog_category": "E", "kegg_orthology_id": "K00600" }, "pscc": { "uniref50_id": "UniRef50_P56089", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_P56089" ], "product": "Serine hydroxymethyltransferase" }, "genes": [ "glyA" ], "id": "GLJLFKCOHC_1090", "locus": "GCA013368035_01091" }, { "type": "cds", "sequence": "contig_1", "start": 633328, "stop": 633624, "strand": "+", "frame": 1, "gene": null, "product": "Asp23/Gls24 family envelope stress response protein", "db_xrefs": [ "RefSeq:WP_012855740.1", "SO:0001217", "UniParc:UPI0001BC24B5", "UniRef:UniRef100_D1J8Q4", "UniRef:UniRef50_A0A3G8LGV6", "UniRef:UniRef90_D1J8Q4" ], "nt": "ATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACCAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAA", "aa": "MILAEIKNLIKSSISIIPGIVDFVELTSTNNINIENDGLIIEEDPQNKNIINITIGLRIMSNLSAKNIVEGIYEIIPYNLTSYDIKLGTLTIYIKGTK", "aa_hexdigest": "7f63b54d2cc3e32b5b5d28cdfbd465c3", "start_type": "ATG", "rbs_motif": "AGxAG", "ups": { "uniparc_id": "UPI0001BC24B5", "ncbi_nrp_id": "WP_012855740.1", "uniref100_id": "UniRef100_D1J8Q4", "db_xrefs": [ "SO:0001217", "UniParc:UPI0001BC24B5", "RefSeq:WP_012855740.1", "UniRef:UniRef100_D1J8Q4" ] }, "ips": { "uniref100_id": "UniRef100_D1J8Q4", "uniref90_id": "UniRef90_D1J8Q4" }, "psc": { "uniref90_id": "UniRef90_D1J8Q4", "uniref50_id": "UniRef50_A0A3G8LGV6" }, "pscc": { "uniref50_id": "UniRef50_A0A3G8LGV6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3G8LGV6" ], "product": "Asp23/Gls24 family envelope stress response protein" }, "genes": [], "id": "GLJLFKCOHC_1091", "locus": "GCA013368035_01092" }, { "type": "cds", "sequence": "contig_1", "start": 633753, "stop": 635252, "strand": "+", "frame": 3, "gene": "fakA", "product": "DhaL domain-containing protein", "db_xrefs": [ "COG:COG1461", "COG:I", "SO:0001217", "UniRef:UniRef50_N9TTI7", "UniRef:UniRef90_D1J8Q5" ], "nt": "ATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCTGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCTTCTAAAAAATCTGCGAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGAGTTACTGATTCGGGTGGCGAAGGTTTATATGCAATATTGCAAGGAATGAGCGCATATTTTAGCGGTAATAAAGTAGAACCTATTGTTACAGAAGAGTCAGTTGAAAAATTTATTTCTGATGAAGAAGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTGGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTAGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTTGTAAAAATTAAAATAGATAACATGACTTTGCAAGCAAACAATAGTAAGAAAACCGCAAAGAAAAAACAAACAGAAGCACCAAGCAATATTGAATTAAAAAATAGCGCCATTGTTTCATGCAATACAGGCCAAGGAATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGTGGTCAAACAAATAATCCTTCAATTCAAGATATTGTTAATGCAATAAATGCAGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGTACTATTATTAGAGATAAGAATGTAATTATTATTCCAACTAGAAGTCAAGTTCAAGGATTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCACGAATTAATGAGCAATAGTTTAAAACACATAAGATATGGAGAAATTGCTGCTGCTATAAAAGATGCTAAATTAAACGGAATAAAAGTTAAAGCATCCGATTACATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTATCTGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAACGATGCGTTGGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGCGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAG", "aa": "MSSTISSACNSECLEDDCSIESASRIVAQNMLMSARGNSGVILSQIFKGFSNGFASKKSANAFDLVNAFEMAAKSAYGAVLKPVEGTILTVIREVSEGLKNQVVVDTTIEEVFEIAVKLARKSCDGTPKLLKILKEVGVTDSGGEGLYAILQGMSAYFSGNKVEPIVTEESVEKFISDEEVYEGEFGYCTEFILELYKPKKFDKDYLVRKLEKIGNSMVVVNDDKILKVHIHTPKPGDVLNSVNSLGQFVKIKIDNMTLQANNSKKTAKKKQTEAPSNIELKNSAIVSCNTGQGIVTLMKENGVSYVVDGGQTNNPSIQDIVNAINAVNAKTVFVLPNNSNIILSAQQAGTIIRDKNVIIIPTRSQVQGLNVALNYSSENSDEENHELMSNSLKHIRYGEIAAAIKDAKLNGIKVKASDYMVIVEGKLQKTSKSLFNAAQLLVSELVSDDSQVVTIIYGQDVSENDALELQNYIETTYDLVVEIYQGDQGIYPYLISVE", "aa_hexdigest": "b0f691aa817262b3ebdc41858c9be310", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_D1J8Q5", "query_cov": 1.0, "subject_cov": 0.9206642066420664, "identity": 0.992, "score": 928.0, "evalue": 0.0, "valid": true, "gene": "FakA", "product": "DhaL domain-containing protein", "uniref50_id": "UniRef50_N9TTI7", "cog_id": "COG1461", "cog_category": "I" }, "pscc": { "uniref50_id": "UniRef50_N9TTI7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9TTI7" ], "product": "DhaL domain-containing protein" }, "genes": [ "dhaL", "fakA" ], "id": "GLJLFKCOHC_1092", "locus": "GCA013368035_01093" }, { "type": "cds", "sequence": "contig_1", "start": 635264, "stop": 635743, "strand": "+", "frame": 2, "gene": "plsX", "product": "phosphate acyltransferase PlsX", "db_xrefs": [ "COG:COG0416", "COG:I", "EC:2.3.1.274", "GO:0008610", "GO:0043811", "SO:0001217", "UniRef:UniRef50_D1J8Q6", "UniRef:UniRef90_D1J8Q6" ], "nt": "ATGAAAAAAATAGTATTTGATTTATTAAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTGCAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAGAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTAAGAACTCAAAATTCAATGCTGGAAGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGTGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCTATTCTACCAACAACAACGGATAAAAAAATGTTATTAATAGATGCGGGGGCAAACATTGAAATCAAACCAGAATATTTAAGCCAATGGGCTATGATAGCAAGTGAATGA", "aa": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTDKKMLLIDAGANIEIKPEYLSQWAMIASE", "aa_hexdigest": "922e9b1b0cb019f893741adff7fa6e4f", "start_type": "ATG", "rbs_motif": "AGGA", "genes": [ "plsX" ], "truncated": "3-prime", "pseudogene": { "start": 635264, "stop": 636046, "observations": { "mutation": [ 635741, 636029 ], "stop": [ 635741, 636029 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q6", "query_cov": 1.0, "subject_cov": 0.4746268656716418, "identity": 0.9940000000000001, "score": 304.0, "evalue": 5.72e-102, "gene_start": 1, "gene_end": 159, "reference_start": 1, "reference_end": 159, "reference_sequence": "MKKIVFDLLNNDNGEEQAILGAKRFLQENSDYFLILVGHEQLIKKHLSDYIDRIAIINNETIVHIESSPRDVLRTQNSMLEAFKQLNESNADGILSSGDSGSLITLSSLKVKRLLGIERPAFMPILPTTTNKKMLLIDAGANIEIKPEYLSQWAMIASEWYSILFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKNWKTKLAALILKRHLREFKQKFDYRNTGGAFIIGLEKIVVKAHGGSDELAFYNALNQIKIGIENNISTILNKKFKEADNEE" }, "paralog": false, "identity": 0.9884615384615385, "score": 494.0, "evalue": 3.25e-179, "description": "Internal stop codon at 635741, 636029. Nonsense mutation around 635741, 636029.", "psc": { "uniref90_id": "UniRef90_D1J8Q6", "gene": "plsX", "product": "phosphate acyltransferase PlsX", "ec_ids": [ "2.3.1.274" ], "uniref50_id": "UniRef50_D1J8Q6", "cog_id": "COG0416", "cog_category": "I", "go_ids": [ "GO:0008610", "GO:0043811" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q6", "EC:2.3.1.274", "UniRef:UniRef50_D1J8Q6", "COG:COG0416", "COG:I", "GO:0008610", "GO:0043811" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q6" ], "product": "Phosphate acyltransferase" } }, "id": "GLJLFKCOHC_1093", "locus": "GCA013368035_01094" }, { "type": "cds", "sequence": "contig_1", "start": 635753, "stop": 636031, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGGAAGCAAAAAGCCAATAGTAGGACAATTAAACGTAGGCGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGAACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGA", "aa": "MFGSKKPIVGQLNVGVEEYKGSEITKNAKNILKEVSKSANFVFNGYVEATNAINGDIDILVVDGYGGNVFLKTLEGTFIAFSKLLKNILLKN", "aa_hexdigest": "59450b628a35b04801e5ffb8ffc405e5", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9999.448399999992, "isoelectric_point": 8.892075920104983 }, "id": "GLJLFKCOHC_1094", "locus": "GCA013368035_01095" }, { "type": "cds", "sequence": "contig_1", "start": 636261, "stop": 636794, "strand": "+", "frame": 3, "gene": "rnc", "product": "ribonuclease III", "db_xrefs": [ "COG:COG0571", "COG:K", "EC:3.1.26.3", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396", "SO:0001217", "UniRef:UniRef50_D1J8Q7", "UniRef:UniRef90_D1J8Q7" ], "nt": "ATGAAGAATAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAGAATTAAATCAACAAAAGAGACATTATTTTTATAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTTATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCAACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTTTTATCGAAATATTATGGTTTTCCAAATTGTTTGAGAACAGGTAAAAGTGCATTTGCAAACGGAAAAGGAATCAAATCAGATTCTGATCTAATTGAATCATTCATTGGTGCTTGTTATAAGGCCTACGGTTTGGAATTTACTAAAAAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGA", "aa": "MKNNFEENLQNALLKFGINFQELNQQKRHYFYNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSKYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKAYGLEFTKKIIYHFIDKILKKFHNSINEHPKTKLQELVQSN", "aa_hexdigest": "2d75ccc18e07ba1ed5607e998fbb793b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [ "rnc" ], "truncated": "3-prime", "pseudogene": { "start": 636261, "stop": 636956, "observations": { "mutation": [ 636792 ], "stop": [ 636792 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8Q7", "query_cov": 1.0, "subject_cov": 0.7662337662337663, "identity": 0.955, "score": 337.0, "evalue": 2.9e-116, "gene_start": 1, "gene_end": 177, "reference_start": 1, "reference_end": 177, "reference_sequence": "MKNNFEENLQNALLKFGINFQKLDEQKRRYFCNAFTHSSYSNEHKNLESYQYLEFLGDSVLGFVVSDFIFDNYKNFGEGKATKLKSEIVDKNALGFLSRYYGFPNCLRTGKSAFANGKGIKSDSDLIESFIGACYKACGLEFTKEIIYHFIDKILKKFHNSINEHPKTKLQELVQSNWGCRPRYEDQALNNGKFESKVFINNNLYGEGIGQSKKESQTKAANDALKKMAKI" }, "paralog": false, "identity": 0.9523809523809523, "score": 448.0, "evalue": 1.91e-162, "description": "Internal stop codon at 636792. Nonsense mutation around 636792.", "psc": { "uniref90_id": "UniRef90_D1J8Q7", "gene": "rnc", "product": "ribonuclease III", "ec_ids": [ "3.1.26.3" ], "uniref50_id": "UniRef50_D1J8Q7", "cog_id": "COG0571", "cog_category": "K", "go_ids": [ "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8Q7", "EC:3.1.26.3", "UniRef:UniRef50_D1J8Q7", "COG:COG0571", "COG:K", "GO:0003723", "GO:0004525", "GO:0006364", "GO:0006396" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8Q7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8Q7" ], "product": "Ribonuclease 3" } }, "id": "GLJLFKCOHC_1095", "locus": "GCA013368035_01096" }, { "type": "cds", "sequence": "contig_1", "start": 636978, "stop": 639914, "strand": "+", "frame": 3, "gene": "smc", "product": "chromosome segregation protein SMC", "db_xrefs": [ "COG:COG1196", "COG:D", "GO:0005524", "GO:0007059", "GO:0030261", "RefSeq:WP_175949970.1", "SO:0001217", "UniParc:UPI001589A103", "UniRef:UniRef100_UPI001589A103", "UniRef:UniRef50_A0A448ZX91", "UniRef:UniRef90_D1J8Q8" ], "nt": "ATGAAATTAATTCAAGTTGAGGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCAATAATAGGACCAAATGGGTCAGGAAAATCAAACATAAACGATGCAATCAGATGGGTGTTAGGTGAAACTAGTTCCAAAGTGTTGCGTGGAGATACAATGGAAGACGTTATCTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACTAGGGGAAATGGTTCAAATGAATATTACATTAATGGTGAATTAGCAAGACAAAGAGATATAAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCAAGTTTAGCAATCATAGGTCAAGGAACTATTTCTAACATTGCCGAATCTACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCCGGAACATCAATGTACAAAAGCAAGAAGATTGAAGCGCAAAGAAAATTAGAAAAGACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAACAATTAAAAGAAGTTGAAGTGGCATTATTGGTTCATGATTTCATGGATTATTCAGATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATTTAATTAAAGAAGAATTAGAAACTAAAATAAGAGTATACAACGAAGCAAGCGAACAAAAAAGCAAGTTTGTTTTAGAATTAGAGAATAACATTAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACTCTAGAAATAAGAAACGCAAAAGAAGCAAAACACCGCGAAATGCTTTTAAGTGGCGAATTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTACTATTTTAGGGTATAAGACATTCTTAGAAACTAAAAAGAAGGAATCTGAGCAATTATCTCAATCAGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCTTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAAACAAATTTAGCTAAAGGAACACATAATATTGTTGAAAATTCTCATTTATTTAGGGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAGATATGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGCGGTAGAGCAACTTTTATTCCCTTATCATCAATTAAACCAAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCTACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTTTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCTAAATTGCTAGAAACTCGTTACATGGTCGTAACGCTAGATGGCGACATCATAAGAGTTGGTGGGGTTCTTTCAGGTGGACAAGCAAGTCAAAATGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCAAAAATTTCACAATTATCAAATGAGTTGTTTGAATTAGAAAAAGACCAAAACAAAGAAACATCGTTATTAACAAATATTTCTCTTGAAAGAGCAAAGATTGATAATTTATATAGAACAACATTGGATAAGTTTGATTCATTGCGTGAACAATATCGTAGTGCTTCGCAAGAAGAATATGAAGCAGAAAACAATAAAAAAATTGATATAGTAGCAGCAATTCAACAGTTGATGCTTGAAAGAAGTGATTTATCGGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTGATTAAAGATAACGCTAACAAAATAGCAGAAAAAGCAAAAGCAGAAACAATTATTCAAAATGCAAAACAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTATAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTAGATATAAGAGAGTTAGGGCACGTAAACCTTGATTCAATTAACCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCAGCAAAAACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACGCAAACAGTTACATTAGTCAATGCCGAATTCAAGCATGTGTTTGCAAAAATGTTTGGCGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGTGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGATGAAGTTGAAGCAGCCCTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTACACACTCTAAAAAAAGATACTCAATTTATAGTGATTACTCACCGTCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGTGCAACGATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGATGCAAAGAAAACTATTGAAAACAACAATTAA", "aa": "MKLIQVEAHGFKSFADKVTLKFDGGIVAIIGPNGSGKSNINDAIRWVLGETSSKVLRGDTMEDVIFSGSKTEKEMDRAEVILTFDNKDRSCDIPYDVFTISRVLTRGNGSNEYYINGELARQRDIKEIAMQSGISKSSLAIIGQGTISNIAESTPEKRREIFEDAAGTSMYKSKKIEAQRKLEKTQEALEQISILIQEQERQLKPLQRQAEKAKIYKTKAEQLKEVEVALLVHDFMDYSDKLEKLNVEGQDYNLIKEELETKIRVYNEASEQKSKFVLELENNIKKISERLSEISEEITTLEIRNAKEAKHREMLLSGELTISPKEKLAAMKEELNALNTTILGYKTFLETKKKESEQLSQSASIRSTKINDIKRELSFKKEELNKIKSKLDVIEEIKSKKTNLAKGTHNIVENSHLFRGYKALVSELIEVNERYALAIETILSSAMQHIVVDKPETAVEAINFLKSNNGGRATFIPLSSIKPKFVPEQHIVVAQTQKGFLGIASELVSTEEQYNVLKKFLLGNVLVADNIENANKLSKLLETRYMVVTLDGDIIRVGGVLSGGQASQNVSTLAADEQVKKLQDLIPVINAKISQLSNELFELEKDQNKETSLLTNISLERAKIDNLYRTTLDKFDSLREQYRSASQEEYEAENNKKIDIVAAIQQLMLERSDLSAKKSSQEDIYHNLKQELQNLTNQRFQVSEELNNLIKDNANKIAEKAKAETIIQNAKQRLSEQYGMLFEVAKQFYNPDIDFEVARKTVADLKLDIRELGHVNLDSINQLEELETRYKEVKSQEQEIISAKTTIEDAISEMDKIIIERMTQTVTLVNAEFKHVFAKMFGGGMAEIRYTDPENILETGIDVIAQPPGKSIKNLKLFSGGEKALIAISLLFSILKAKPLPLCILDEVEAALDEANVIRFAEYLHTLKKDTQFIVITHRQGTMERVNKLYGATMQKRGVTTFFAVNLSDAKKTIENNN", "aa_hexdigest": "9cb16a1776306a89b74d2544b6af2bf9", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "ups": { "uniparc_id": "UPI001589A103", "ncbi_nrp_id": "WP_175949970.1", "uniref100_id": "UniRef100_UPI001589A103", "db_xrefs": [ "SO:0001217", "UniParc:UPI001589A103", "RefSeq:WP_175949970.1", "UniRef:UniRef100_UPI001589A103" ] }, "ips": { "uniref100_id": "UniRef100_UPI001589A103", "uniref90_id": "UniRef90_D1J8Q8" }, "psc": { "uniref90_id": "UniRef90_D1J8Q8", "gene": "smc", "product": "chromosome segregation protein SMC", "uniref50_id": "UniRef50_A0A448ZX91", "cog_id": "COG1196", "cog_category": "D", "go_ids": [ "GO:0005524", "GO:0007059", "GO:0030261" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZX91", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZX91" ], "product": "Chromosome partition protein Smc" }, "genes": [ "smc" ], "id": "GLJLFKCOHC_1096", "locus": "GCA013368035_01097" }, { "type": "cds", "sequence": "contig_1", "start": 640143, "stop": 640811, "strand": "+", "frame": 3, "gene": null, "product": "Variable surface lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZ40", "UniRef:UniRef90_A0A6A8PZ40" ], "nt": "ATGAAAAAGTCTAAGAAAATATTATTAACAATAGGAATTGCATTTCCACTTTTAGCTTCAACTCCATTAGTTGCTGCCGGATGTGTAGAAACAAATAAACCAGAAGATAAAAAACCAGGAACAACTCCTGAAAAGAAGCCAGAAGATAAAAAACCAGGAACAACTCCTGAAAAGAAGTCTGAAGGTGAAACCACAATAGAAAATATCATTAATAAACATAAAGAAATTAAAGATGATGAATTAAACAATACTAAATCATTATTAGATCAATTTAAAAAATCAATTGAAGAAAGAACAAATCAAGCAGTATTAAACGAAGCAAAGAATCAATTAAATTCAGCTTTAAAACATGCGGTAAAAATAAATACAAAGTCATTTAATTTTAACCAAAAATTTAATTTTAATAAAGAATATGTGAAGGGATTAGAAGCAACTAAAGGTAACGATTTGAATGTTATTAAAGAAATTACTTTCAAATTAAATAGCTTTATAAATTTAGCAACATCACAAAAACCAAAAACGGCAACAGAAATTCAAAATCATGTTGAAGGCGAAGTTCATGGATTAAATGATTTAATATTAAAATTGGATGATTTTGTTTCTGAATATGTTGAAGAAACATTTAATGATATAGATGAAGCAGGTTTAATTAGCTCAATAGATAATTGA", "aa": "MKKSKKILLTIGIAFPLLASTPLVAAGCVETNKPEDKKPGTTPEKKPEDKKPGTTPEKKSEGETTIENIINKHKEIKDDELNNTKSLLDQFKKSIEERTNQAVLNEAKNQLNSALKHAVKINTKSFNFNQKFNFNKEYVKGLEATKGNDLNVIKEITFKLNSFINLATSQKPKTATEIQNHVEGEVHGLNDLILKLDDFVSEYVEETFNDIDEAGLISSIDN", "aa_hexdigest": "42dc98255707f08ba379c573f6feac16", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 640143, "stop": 641114, "observations": { "mutation": [ 640809, 640929 ], "stop": [ 640809, 640929 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_A0A6A8PZ40", "query_cov": 1.0, "subject_cov": 0.6288951841359773, "identity": 1.0, "score": 369.0, "evalue": 3.96e-126, "gene_start": 1, "gene_end": 222, "reference_start": 1, "reference_end": 222, "reference_sequence": "MKKSKKILLTIGIAFPLLASTPLVAAGCVETNKPEDKKPGTTPEKKPEDKKPGTTPEKKSEGETTIENIINKHKEIKDDELNNTKSLLDQFKKSIEERTNQAVLNEAKNQLNSALKHAVKINTKSFNFNQKFNFNKEYVKGLEATKGNDLNVIKEITFKLNSFINLATSQKPKTATEIQNHVEGEVHGLNDLILKLDDFVSEYVEETFNDIDEAGLISSIDNWIDMVKDFIKESKNPSESLYFKLKYLKSQTKTFYEQKMESWANKVSDLLIEKGYDLLDKIAHIETYYSEKKMPMVESLAKYREELEDCLNKFAELDETNAVEKQLELSKKLYDLITKIQKELNNEYNEDLK" }, "paralog": false, "identity": 0.9938080495356038, "score": 578.0, "evalue": 6.8e-211, "description": "Internal stop codon at 640809, 640929. Nonsense mutation around 640809, 640929.", "psc": { "uniref90_id": "UniRef90_A0A6A8PZ40", "product": "Variable surface lipoprotein", "uniref50_id": "UniRef50_A0A6A8PZ40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_A0A6A8PZ40", "UniRef:UniRef50_A0A6A8PZ40" ] }, "pscc": { "uniref50_id": "UniRef50_A0A6A8PZ40", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A6A8PZ40" ], "product": "Variable surface lipoprotein" } }, "id": "GLJLFKCOHC_1097", "locus": "GCA013368035_01098" }, { "type": "cds", "sequence": "contig_1", "start": 640818, "stop": 640931, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTAAAGATTTTATTAAAGAATCTAAAAATCCTTCTGAATCGCTATATTTTAAATTAAAATATTTGAAATCGCAAACAAAAACATTTTATGAACAAAAAATGGAATCATGA", "aa": "MVKDFIKESKNPSESLYFKLKYLKSQTKTFYEQKMES", "aa_hexdigest": "f85d6bc0df3c9402ff335ce290fd30a0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4525.202000000001, "isoelectric_point": 9.347996330261232 }, "id": "GLJLFKCOHC_1098", "locus": "GCA013368035_01099" }, { "type": "cds", "sequence": "contig_1", "start": 640977, "stop": 641204, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGATAAAATAGCACATATTGAAACATATTATAGTGAAAAGAAAATGCCAATGGTTGAAAGCCTTGCTAAATATCGTGAAGAATTGGAAGATTGCTTAAATAAATTTGCTGAACTAGATGAAACAAATGCCGTGGAAAAACAACTTGAGTTGAGTAAAAAACTTTATGATTTAATAACAAAAATTCAAAAAGAATTAAACAACGAATATAACGAAGACCTTAAATAA", "aa": "MDKIAHIETYYSEKKMPMVESLAKYREELEDCLNKFAELDETNAVEKQLELSKKLYDLITKIQKELNNEYNEDLK", "aa_hexdigest": "5dee2c9f7540144b0e1ceca303a7f286", "start_type": "TTG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8990.135499999995, "isoelectric_point": 4.7701215744018555 }, "id": "GLJLFKCOHC_1099", "locus": "GCA013368035_01100" }, { "type": "cds", "sequence": "contig_1", "start": 641448, "stop": 641567, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGA", "aa": "MYGLVFYLDKEILKKEYSDSNHKEAYEKVRDILRHYGFH", "aa_hexdigest": "3e2077c335083dcd249c2a105b696001", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4836.4392, "isoelectric_point": 6.817859840393066 }, "id": "GLJLFKCOHC_1100", "locus": "GCA013368035_01101" }, { "type": "cds", "sequence": "contig_1", "start": 641941, "stop": 642618, "strand": "-", "frame": 2, "gene": null, "product": "Leucine-rich repeat domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0", "UniRef:UniRef90_D1J8R0" ], "nt": "TTGCTAGATTTATCAAAAACCAATATAACTTCAATACCCGCAGGTGCATTTTCTTATTTAGTTATGCAAAATTTAGCAGACGATGGTGCCGGCTTACCAACCGAATTAAAAACATTAGTTACAACTAATTCTTCAAATAACAACAAAAAAATAAATATTAGAAAAATTATTTTGCCAAAAACATTAAAATCAATTGGCGCAGGTGCTTTTTATGGATTAAGTAACCTAGAAGAAGTAGATTTTTCTCAATGCAACGACTTATCTCAAATAGATGCTGAAGCTTTTTCAAATACAAATATTAAAAAATTACAATTGCCTGCATCTTTAAATAAGTTAGGACAAAAAGCCTTTGCAAACAGTGGCATCACATCTGTAAATTTAGAAGATTTACCTTATTTAAAAACTCTTACACAAGGTGTTTTTGCTTCAAATAAGCTTACAAACATAAACTTAAAAAACATTGAATTAGTTGAAAGTGGAGCTTTGGCAGATAATCAAATAAAAACAATAGAAATTCCTTCAACAATGAAATCATTTAGTCCTGAATCTTTTGACTTTTACGGTGCTGGGGAATCAGCTGCTAAAACTCTTGTTTCTAAAATTGAAAACAATGAAATTAAATCATTTTTAAAAGATGCTTTGAAGGAAAACCCAAATCATCTATATAAATTAAGCTAA", "aa": "MLDLSKTNITSIPAGAFSYLVMQNLADDGAGLPTELKTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTNINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFYGAGESAAKTLVSKIENNEIKSFLKDALKENPNHLYKLS", "aa_hexdigest": "8e29f4beec80e648fedc4e18758f2e16", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 641941, "stop": 642804, "observations": { "mutation": [ 642636 ], "stop": [ 642636 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8R0", "query_cov": 1.0, "subject_cov": 0.7839721254355401, "identity": 0.9640000000000001, "score": 390.0, "evalue": 2.79e-135, "gene_start": 1, "gene_end": 225, "reference_start": 63, "reference_end": 287, "reference_sequence": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNHVQGPLKDPKNSTNHNQTWFAESGLLDLSKTNITSIPAGAFSYLVMQNLADEGAGLPTELSTLVTTNSSNNNKKINIRKIILPKTLKSIGAGAFYGLSNLEEVDFSQCNDLSQIDAEAFSNTNIKKLQLPASLNKLGQKAFANSGITSVNLEDLPYLKTLTQGVFASNKLTNINLKNIELVESGALADNQIKTIEIPSTMKSFSPESFDFIGAGESAAKTLISKIENHEIESFLKDALKKNPNHLYKLS" }, "paralog": false, "identity": 0.9651567944250871, "score": 513.0, "evalue": 3.5e-186, "description": "Internal stop codon at 642636. Nonsense mutation around 642636.", "psc": { "uniref90_id": "UniRef90_D1J8R0", "product": "Leucine-rich repeat domain-containing protein", "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8R0", "UniRef:UniRef50_D1J8R0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8R0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R0" ], "product": "Leucine-rich repeat domain-containing protein" } }, "id": "GLJLFKCOHC_1101", "locus": "GCA013368035_01102" }, { "type": "cds", "sequence": "contig_1", "start": 642634, "stop": 642804, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAGAACAAAAAATTTTTATTTTTAGGCTTTGGTTTAGTTGCTCCATTTATTGCTGGATTAACAGCAATATCTTGTGGAGGAGAAAAGTTCAATGCTTTTGCTCTAAACTATGTACAAGGACCGCTAAAAGACCCTAAGAACTCAACCAATCACAATCAAACTTGA", "aa": "MKKNKKFLFLGFGLVAPFIAGLTAISCGGEKFNAFALNYVQGPLKDPKNSTNHNQT", "aa_hexdigest": "7feac059743a39b3b7b2d704eccb07de", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6088.023000000003, "isoelectric_point": 9.809396553039551 }, "id": "GLJLFKCOHC_1102", "locus": "GCA013368035_01103" }, { "type": "cds", "sequence": "contig_1", "start": 642898, "stop": 644868, "strand": "+", "frame": 1, "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "db_xrefs": [ "COG:COG0556", "COG:L", "EC:3.1.25.-", "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887", "RefSeq:WP_175949971.1", "SO:0001217", "UniParc:UPI00158C4063", "UniRef:UniRef100_UPI00158C4063", "UniRef:UniRef50_B3PLX9", "UniRef:UniRef90_D1J8R1" ], "nt": "ATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCTGCCGGCGACCAACCACAGGCAATAAATGAATTGGTTCACGGTGTTTTAGAGGGAAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACCATGGCCAATGTCATTAATCAATTAAATAGGCCTACATTAGTTATAAGTCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCCGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCATACATTCCTAAGTCTGATTTATATATCGAAAAAACAAGTAGGAACAATAAAGATTTAGAAGCAATGAGAATGTCTGCTCTTAATGCTTTAAGTATCCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCTGAATTTAACCCAAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTGCGTGATCTTTTAACCAAGTTGGTTAATTTAGGTTATGAAAAGCATAATGGTGATTTATCTTCTGGACAATTTTTTAATAAAGGGGAAGTTATTGAAATTTGTCCTGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTGCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAATGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGCGAATTAAAAGAAAGAGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCACAAAGGATTCATGATAAAACTTTAAATGATATTGATTCATTAAAGGAATTCGGTTATGTTAAAGGCATTGAAAATTACGCTCGATATGTTGATAAACGTGAACCCGGACAAAAACCATACACGATATTTGATTATTTGCCAAAGGATGCAGTAATTTTTGTTGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTATGAAGGAGACCGTTCTAGAAAAGAAACACTAATTAAATATGGTTTTAGATTACCTTCTGCATTAGACAATAGACCCTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACTCCTGGTGACTATGAAATAAATAAAGCAAATGGTGAACTAGTTACACAATACATTAGACCAACAGGTCTTTTAGATCCCGTTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGATTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTGACTACAACTAAAAAGAATGCTGAAGAGCTTTCTTTGTATCTTTCTGAATTTAAAATCAAAAGCGCATATATTCATGATGGATTTAAAACCTTTGAAAGAAATGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTTAGTTTGATTTGCGTATTGAATGCAGATTCTATGGGGCTAATGCGCGATGCTAGAAGTTTGATTCAAATAGTTGGCAGGGCCTCTAGAAACGACCATGGAAAAGTAATATTTTATGCCGATAATATTACAACCAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGGACGTTGCAAATTAAATATAATAAAGAACACAACATTACCCCTAAAAGTATTATTAAACCAATACCCGCACCTATTCAAGGCAGTATTTTATTAGAAGATAATAAAGCAACAAAAGAAGCCAATAGAACTATTCATAATAAAAAATATATTGATAATATGATTAAACATATGAAAGAACTAGCAAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGGGATTATTTGATTGAATTAGGTGTCGAATTTGATGATTAA", "aa": "MEKFKLVSNYKPAGDQPQAINELVHGVLEGKEHQVLLGVTGSGKTFTMANVINQLNRPTLVISHNKTLASQLYSELRELFPENRVEYFVSYFDFYKPEAYIPKSDLYIEKTSRNNKDLEAMRMSALNALSIRRDTIVVASVAAIYAEFNPKEYIQSFMPIESHMKIKLRDLLTKLVNLGYEKHNGDLSSGQFFNKGEVIEICPGYTSENNIRIEMFDDYIERIALINPLTKSIVEVRKSLTIFPATTYNVEKGYIENICNSIESELKERVAYFKSQNQLLEAQRIHDKTLNDIDSLKEFGYVKGIENYARYVDKREPGQKPYTIFDYLPKDAVIFVDESHLMIPQIIGMYEGDRSRKETLIKYGFRLPSALDNRPLKFNEYEEYKFPRIYMSATPGDYEINKANGELVTQYIRPTGLLDPVIEIHSKENQIQDIHDLLKLQIEKKERTLILTTTKKNAEELSLYLSEFKIKSAYIHDGFKTFERNEILKGLRMGKYDVVIGINLLREGIDLPEVSLICVLNADSMGLMRDARSLIQIVGRASRNDHGKVIFYADNITTSMQKAIVDNEHKRTLQIKYNKEHNITPKSIIKPIPAPIQGSILLEDNKATKEANRTIHNKKYIDNMIKHMKELARAKKYEEAIEIRDYLIELGVEFDD", "aa_hexdigest": "a2ff83422562b079f0c49526ef44f8d0", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158C4063", "ncbi_nrp_id": "WP_175949971.1", "uniref100_id": "UniRef100_UPI00158C4063", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158C4063", "RefSeq:WP_175949971.1", "UniRef:UniRef100_UPI00158C4063" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158C4063", "uniref90_id": "UniRef90_D1J8R1" }, "psc": { "uniref90_id": "UniRef90_D1J8R1", "gene": "uvrB", "product": "excinuclease ABC subunit UvrB", "ec_ids": [ "3.1.25.-" ], "uniref50_id": "UniRef50_B3PLX9", "cog_id": "COG0556", "cog_category": "L", "go_ids": [ "GO:0003677", "GO:0005515", "GO:0005524", "GO:0006289", "GO:0009380", "GO:0016787", "GO:0016887" ] }, "pscc": { "uniref50_id": "UniRef50_B3PLX9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_B3PLX9" ], "product": "UvrABC system protein B" }, "genes": [ "uvrB" ], "id": "GLJLFKCOHC_1103", "locus": "GCA013368035_01104" }, { "type": "cds", "sequence": "contig_1", "start": 645039, "stop": 645320, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAAACCAATATAAAATGGAGTTTTTTTATACTATTAGCAATTATTTTTATAATTGCTTTTGCTTTAATATTAACTAGCTGTGCCTCAACTGATCCTAGAGATGCACAGTTTTATATTAGGGATGATAGCTTTTATTACAATAGTAGCGCAAACATAACAAAAGTTAATTTTCAAGTAAGTTTTGAGAATAATTCAATATATAAAATTGTTAAGAATTCAATAACATTTGATTTGTATGATAATGGAAAATATGTATTTCAAAACTCTTTTAATTGA", "aa": "MKKTNIKWSFFILLAIIFIIAFALILTSCASTDPRDAQFYIRDDSFYYNSSANITKVNFQVSFENNSIYKIVKNSITFDLYDNGKYVFQNSFN", "aa_hexdigest": "31eb05e8a183a4981f471ed7f65fd11a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10868.274199999993, "isoelectric_point": 8.732710075378417 }, "id": "GLJLFKCOHC_1104", "locus": "GCA013368035_01105" }, { "type": "cds", "sequence": "contig_1", "start": 645780, "stop": 646271, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00004.35" ], "nt": "GTGATTAGTTATAATTGCGATGAAGTAATGAATGAATACACTAAAAATCAATTAAATCAAGTTTATAAGCATGTGCTCTTAGATATATGCAAGGCTTCTGAATTTAAAGTTGATGAAAAAATAAAAAAAGAAGAATTAATAAATTTAATTTTGTCAAGAAGACCAGATTTTAAAGAAAGCGACAACACAAAAAAATTTTCTAAACCAAACGATAAAGAAATTAAAACATTAAAAAGCCCCCCCCCCCCTTCTAAAAAGCCGATGTTTGAAGACATTGTGGGTTTAAATAAAGCAAAAGAAGCGTTTATCGAAAAGATTATTCTTCCTTTTAAACACAAAGAAATATTTGAAAAGTTTAATAAAAAAATAGGTGGCGGCATATTACTATATGGTTTGCCCGGTACAGGAAAAACAATGTTTGCAGAGGCTACTTCTAATGAATTAAATGCTTTATTTATTCCAATAAAGTGTTCTGATATTAAAACTAAGTGA", "aa": "MISYNCDEVMNEYTKNQLNQVYKHVLLDICKASEFKVDEKIKKEELINLILSRRPDFKESDNTKKFSKPNDKEIKTLKSPPPPSKKPMFEDIVGLNKAKEAFIEKIILPFKHKEIFEKFNKKIGGGILLYGLPGTGKTMFAEATSNELNALFIPIKCSDIKTK", "aa_hexdigest": "d05983b092baab9ee81a94973e0c6860", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00004.35", "name": "AAA", "length": 35, "aa_cov": 0.2147239263803681, "hmm_cov": 1.0, "evalue": 2.885347580006958e-09, "score": 32.899879455566406, "start": 127, "stop": 161 } ], "seq_stats": { "molecular_weight": 18699.713899999988, "isoelectric_point": 9.179218101501466 }, "id": "GLJLFKCOHC_1105", "locus": "GCA013368035_01106" }, { "type": "cds", "sequence": "contig_1", "start": 646377, "stop": 646499, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGACAATGCAATTAGTGAAGGCAACGACATAGTCCCACAATTGCTATCCGAAATGCAAGGAATTGATAATTTAAAAAATGAATCAAATATTATTGTTATAGCAGCAACAAATAAACCATGA", "aa": "MDNAISEGNDIVPQLLSEMQGIDNLKNESNIIVIAATNKP", "aa_hexdigest": "7e0735a41115ad7540abd037d63b9e50", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4310.813000000002, "isoelectric_point": 4.0500284194946286 }, "id": "GLJLFKCOHC_1106", "locus": "GCA013368035_01107" }, { "type": "cds", "sequence": "contig_1", "start": 646500, "stop": 646850, "strand": "+", "frame": 3, "gene": null, "product": "26S protease regulatory subunit", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000698C617", "UniRef:UniRef90_UPI000698C617" ], "nt": "ATGATAGATTCGGCATTTCTAAGACCAGGAAGATTTGATGAAAAAATTTATATTCCACTTCCTGACTATGAATCAAGAAAAAAGATGTTTGAGATTCAATTATCTTCGTTGCCAATTGCCGATAATTTCGATTTTGATTATTTAGCAAGCATAACTGAAAATTTTAATGGCGATGATATAAAAGAAGTTTGTGAAAAAATAAAAATGAGTGCTATTAAAAAGAATATAAACAATAATCAAGAACATAAAATAGAAATGGAAGATGTAAAACAAATAGAAAAAGACATTAAATCTTCTGTTAATCTTCAAGATATTCAAAAATTAAAAGAATTTGAAAAAAATTTTTATTAA", "aa": "MIDSAFLRPGRFDEKIYIPLPDYESRKKMFEIQLSSLPIADNFDFDYLASITENFNGDDIKEVCEKIKMSAIKKNINNNQEHKIEMEDVKQIEKDIKSSVNLQDIQKLKEFEKNFY", "aa_hexdigest": "f16e6d72874abc2f2b75de6f07fed373", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 646200, "stop": 646850, "observations": { "mutation": [ 646269, 646497 ], "stop": [ 646269, 646497 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000698C617", "query_cov": 1.0, "subject_cov": 0.5321100917431193, "identity": 0.983, "score": 216.0, "evalue": 1.4e-69, "gene_start": 1, "gene_end": 116, "reference_start": 103, "reference_end": 218, "reference_sequence": "MFAEATSNELNALFIPIKCSDIKTKWYGESEQKIHKIFERARKQEKAIIFFDEFEAIGTKRMDNAISECNDIVPQLLSEMQGIDNLKNESNIIVIAATNKPWMIDSAFLRPGRFDEKIYIPLPDYESRKKMFEIQLSSLPIADNFDFYYLASITENFNGADIKEVCEKIKMSAIKKNINNNQEHKIEMEDVKQIEKDIKSSVNLQDIQKLKEFEKNFY" }, "paralog": false, "identity": 0.9768518518518519, "score": 410.0, "evalue": 1.38e-148, "description": "Internal stop codon at 646269, 646497. Nonsense mutation around 646269, 646497.", "psc": { "uniref90_id": "UniRef90_UPI000698C617", "product": "26S protease regulatory subunit", "uniref50_id": "UniRef50_UPI000698C617", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000698C617", "UniRef:UniRef50_UPI000698C617" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000698C617", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000698C617" ], "product": "26S protease regulatory subunit" } }, "id": "GLJLFKCOHC_1107", "locus": "GCA013368035_01108" }, { "type": "cds", "sequence": "contig_1", "start": 646887, "stop": 647279, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R8", "UniRef:UniRef90_D1J8R8" ], "nt": "ATGCAGGTAAAAAATGCCAAAAAATTTGCGTTAGCTACTATAATATTATATTTTATTTGTGTAGTACTTTTAATACCAATGATTATATTAAATTTGATGCAAGATACAATAAAGGAAAATCTTTCTTTGTTTTCGGCGATAGCTTTGCTTTCGTTTGCATTAGAATATTTAGGAGCTGCTATAACGTTCATAACGTTTTATATAATTACAATTGTAAATGGTTTTTACTATAAGGAAACGCTTGGATCAATATTAATGTGCGTAGGAATTATTGTACCTATTGTAGGAATTATTGGAACGTTTTTTGTTTATTTTAATCTTAAAAAAATACCGGTTGTTAAATTAACCACACCAATCGTTAAACCAACAGTTGATACTCCAAATCCATCATGA", "aa": "MQVKNAKKFALATIILYFICVVLLIPMIILNLMQDTIKENLSLFSAIALLSFALEYLGAAITFITFYIITIVNGFYYKETLGSILMCVGIIVPIVGIIGTFFVYFNLKKIPVVKLTTPIVKPTVDTPNPS", "aa_hexdigest": "02ec5168d2e602e10e1eacb867c598bf", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J8R8", "query_cov": 1.0, "subject_cov": 0.8904109589041096, "identity": 0.9620000000000001, "score": 227.0, "evalue": 1.27e-74, "valid": true, "uniref50_id": "UniRef50_D1J8R8" }, "pscc": { "uniref50_id": "UniRef50_D1J8R8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8R8" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 14426.47379999998, "isoelectric_point": 9.14582347869873 }, "id": "GLJLFKCOHC_1108", "locus": "GCA013368035_01109" }, { "type": "cds", "sequence": "contig_1", "start": 647328, "stop": 647423, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAGAAATAAAATAAAAGAATCTCTAGCGATTGTTATATTGCTCCCTATTATTACTGCCTTGAACTTACTTTTTGTATTTACTAGAGGATGA", "aa": "MKRNKIKESLAIVILLPIITALNLLFVFTRG", "aa_hexdigest": "d9f99be9066e1c128f429feb8c88c941", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3526.369400000001, "isoelectric_point": 11.097023582458494 }, "id": "GLJLFKCOHC_1109", "locus": "GCA013368035_01110" }, { "type": "cds", "sequence": "contig_1", "start": 647853, "stop": 648518, "strand": "-", "frame": 3, "gene": null, "product": "Type-2 restriction enzyme", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S0NKX5" ], "nt": "GTGAATTTCCAAAAAGTTTATGAAAATATTGAAAAAATAAAAATTGAATTATGCATTTTAAATTCATTAATTAAATCAAAAAATATAAAAGCAGATTTTAAAAATTTAATACAAATGTATCCTAATGTATTAAAAGCCATACCAACTTTATTAGCAATAAGAGGTAATAATATTCGAGTTGGAATTGAAAAAGAAATTCTAATTAATTTTGATAAAAAGCTAAATAGTATAGAAGAATATACACTATTTATGGAAAAGACAGGTTTGTTTGAATTAATAAGAAATAATATAAACAATAGCCTTGTTGACTATGCAACTGGTGTTGAAGTGGGAATTGATACAAATACAAGAAAAAACAGAACAGGCTCAATGATGGAAAACCTTGTTGAACATTATTTAATAAATTCAAATTTAATTGAAAATGTTAACTATTTTAAGCAAATTGATTTAAATAACTTTCAAAAGAAAACGAATATTAATATTGACCCCACATTATTAAATATAAATAAAAAATTTGACTTTATTTTTATAAATAATCAAAATCATATTTTTGTATGCGAATGTAATTTTTACAATACAAGTGGTTCAAAATTGAATGAGGTTGCCAGAAGTTATAGGATTTTATCAAGGTTATTTGATGAGACTGGAAATATTACATTTCTTTGA", "aa": "MNFQKVYENIEKIKIELCILNSLIKSKNIKADFKNLIQMYPNVLKAIPTLLAIRGNNIRVGIEKEILINFDKKLNSIEEYTLFMEKTGLFELIRNNINNSLVDYATGVEVGIDTNTRKNRTGSMMENLVEHYLINSNLIENVNYFKQIDLNNFQKKTNINIDPTLLNINKKFDFIFINNQNHIFVCECNFYNTSGSKLNEVARSYRILSRLFDETGNITFL", "aa_hexdigest": "141219749a9f4cd0feee7d4febea7640", "start_type": "GTG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_UPI0030CDD1ED", "query_cov": 0.9638009049773756, "subject_cov": 0.8452380952380952, "identity": 0.507, "score": 195.0, "evalue": 5.04e-59, "valid": false, "product": "type II restriction endonuclease", "ec_ids": [ "3.1.21.4" ], "uniref50_id": "UniRef50_A0A2S0NKX5", "kegg_orthology_id": "K01155" }, "pscc": { "uniref50_id": "UniRef50_A0A2S0NKX5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A2S0NKX5" ], "product": "Type-2 restriction enzyme" }, "genes": [], "id": "GLJLFKCOHC_1110", "locus": "GCA013368035_01111" }, { "type": "cds", "sequence": "contig_1", "start": 648578, "stop": 649294, "strand": "-", "frame": 1, "gene": null, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "EC:2.1.1.72", "KEGG:K06223", "SO:0001217", "UniRef:UniRef50_N9U9M3", "UniRef:UniRef90_A0A6A8Q0M8" ], "nt": "TTGCCTAAGAATTTTTGTATAAATGATATTAATAAAGAACTGGTTCTAGCATACAAGTGTTTTACAAAGAAGGAATCATTTGATAAATTAAAATCACTTTTAGACCTTCATACCAGTAAGCATTGCGACGAATACTATTATCAAATAAGAAATTTAGATAGACAACCCGAATATAAAAACTTGTCAGATGAAGAAAAGGCTGCCAGAATTATTTATTTGAATAAAGCATGTTTTAATGGTTTATATCGTGTAAATAAAAATGGTTTTTTTAATGTTCCATCAGGAAAGAAAGAAAAAGTCAATTGCTATAATAAAGAAAACTATAATTCTATTTTTGAATATTTTTCTAATGTAGATTACAAAATAACAAATTTAAATTTTGAAGATGCAATAAAAGATGCAAAAGCTGGCGACTTGGTTTATTTTGACCCTCCTTATGATACCTTAGAAAATAAAAATTCATTTACTTCTTATTCAAATGAAACCTTTGGAAAAAACGAGCAAATTAAACTTGCTAATGTTTTTAAGGAACTGTCAAAAAAGGGCGTTTATGTAATATTAAGTAATCACAAAACTGATTTTGTTTGCAATTTATACAGGGATTTTAATATTAATATAATTAAAGCAAGAAGAAACATTAATAGAAATGGTAAAAATCGAGGCGATGTCGAAGAGGTAATCATTACTAATTTTAATAAAGAGGTTCTATGCAAATAA", "aa": "MPKNFCINDINKELVLAYKCFTKKESFDKLKSLLDLHTSKHCDEYYYQIRNLDRQPEYKNLSDEEKAARIIYLNKACFNGLYRVNKNGFFNVPSGKKEKVNCYNKENYNSIFEYFSNVDYKITNLNFEDAIKDAKAGDLVYFDPPYDTLENKNSFTSYSNETFGKNEQIKLANVFKELSKKGVYVILSNHKTDFVCNLYRDFNINIIKARRNINRNGKNRGDVEEVIITNFNKEVLCK", "aa_hexdigest": "c3c21b0ff8eb23a31a68a24a5433e077", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A6A8Q0M8", "query_cov": 1.0, "subject_cov": 0.8263888888888888, "identity": 0.966, "score": 466.0, "evalue": 3.38e-165, "valid": true, "product": "Site-specific DNA-methyltransferase (adenine-specific)", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_N9U9M3", "kegg_orthology_id": "K06223" }, "pscc": { "uniref50_id": "UniRef50_N9U9M3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_N9U9M3" ], "product": "Site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "GLJLFKCOHC_1111", "locus": "GCA013368035_01112" }, { "type": "cds", "sequence": "contig_1", "start": 649601, "stop": 649783, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAGAATTATCATTATTTGCAAACGTTGGATATGACAAAAATAAATCATTGTATGAACAAGTTGTGCAATTGATAGAAGGAATAAAAAATACTAATTCGCCCAAAGCAAAATTATTAATTAATGAAATTATTGATTTATACAATTCTTATTTTTTAACCTTTGGAAGTAACGGATATTGA", "aa": "MKELSLFANVGYDKNKSLYEQVVQLIEGIKNTNSPKAKLLINEIIDLYNSYFLTFGSNGY", "aa_hexdigest": "a2d4ddd39945bc4da95159407d27a344", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6850.752199999999, "isoelectric_point": 6.089240837097167 }, "id": "GLJLFKCOHC_1112", "locus": "GCA013368035_01113" }, { "type": "cds", "sequence": "contig_1", "start": 650405, "stop": 651088, "strand": "-", "frame": 1, "gene": null, "product": "MYPU_1760 family metalloprotease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D", "UniRef:UniRef90_UPI001E491E9D" ], "nt": "TTGCAAAAGAGAGTTGAGCTAGTTTTTAATACTTTATTTCATGAATACGCACACCACATTGATAATACATACAACCGAAGTATAAGAGAAAATGATGAGTTTGCCAATAATGATTTAAGCTATAAATTAGCAACTCCTGAACTAAACAAAATAATCAATAAATTTAATGGCAACAACAACAAATTTTTAACCGAATTTAGAAAAACGCTAAACTACGATGGTTCAATGCAAAACGATGAATATTTAGTCAATAAAGAATTATTTAATCCTAAAAACAAAGAAATACCAGTTTATTCAAAATTCAGTCAATATGACTTATTCAAGTTAGCTAACCTCGCCTTAACAAACAAAGAAGTTGAAAAATACAATATTTTAAACAATAAAAACTTTTATTTTAATAATTCAACAAACAATACAGTTTATTATGCAAATTCTACTAATATTGATCAAATAAAATACTTGTTTTCATTTGAGGAACTTTTTGCAAGAGAACTAGTAAAACTTTCGTATTCAGCACATGAAAATTTTCAAATGTTTGACGGTCATTTAACTTTGGATGATTCTTTGAATTTCTTATATTATAAATTAAAAAATGATGATATTTTTCTATCTTCAATTGGTGATGACACACTAAAGATGATTGCCAATTATTATAATAAATATGCAATATATTCAGCCAATTGA", "aa": "MQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQIKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYAIYSAN", "aa_hexdigest": "acf1a384b2029c7844e8b42e7e5cf766", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 650386, "stop": 651388, "observations": { "insertion": [ 650413 ], "mutation": [ 651094 ], "stop": [ 651094 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491E9D", "query_cov": 0.9911894273127754, "subject_cov": 0.5541871921182266, "identity": 0.982, "score": 423.0, "evalue": 1.09e-146, "gene_start": 1, "gene_end": 225, "reference_start": 174, "reference_end": 398, "reference_sequence": "MGNIYYNLGFYINGGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNIQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQSWSLQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQLKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSPIEYLMMN" }, "paralog": false, "identity": 0.987987987987988, "score": 645.0, "evalue": 1.91e-236, "description": "Internal stop codon at 651094. Frameshift due to insertion around 650413. Nonsense mutation around 651094.", "psc": { "uniref90_id": "UniRef90_UPI001E491E9D", "product": "MYPU_1760 family metalloprotease", "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491E9D", "UniRef:UniRef50_UPI001E491E9D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D" ], "product": "MYPU_1760 family metalloprotease" } }, "id": "GLJLFKCOHC_1113", "locus": "GCA013368035_01114" }, { "type": "cds", "sequence": "contig_1", "start": 651092, "stop": 651649, "strand": "-", "frame": 1, "gene": null, "product": "MYPU_1760 family metalloprotease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D", "UniRef:UniRef90_UPI001E491E9D" ], "nt": "ATGAGCAAGCAAAAACAAGGTAGAAAAGCATTTCTACCGAAATGGGCAATATTTATTATAATCTTGGCTTTTATATTAACAGCAGGTATTATTGGCGTCGTAATATATTACATTTTTAATTATGTTAATTATTCAAATGGCAATGGTGGGGGTTTATTTGATGGCCAAAAAAATAAAAATTATTCAATGCCAAATGAAGAAAGATATCTACAAAGTTTCAATGCAAAAACAATTGCCAAAAACAATGTTCAATTAAAGAACAAGAATTATCAATATGGAAATTTAAGTATTTTTGAATATCCTTTTGCTTTAACAAAAAATAATGAACCTATTTATTTTTTGGGTGAAGAAGGAAGAAAATTATTGAATTTAGAGTTTTTAAAAAGAGGCTTTTATGGTCCTGAAATTAATGGTTTAAATCATGTTTATATAAATAAAAAAATACCTAATGTTTCAAATATTAAAGATGTTAATGGTGTTTATTTGCCACAAGCTTTTGACATTTTTATTCAAATAAATCATTTTATCTCTGGGGGAATAAAACAAGTCCAATCATGA", "aa": "MSKQKQGRKAFLPKWAIFIIILAFILTAGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIAKNNVQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQS", "aa_hexdigest": "e0ff13611bfd0e781c26a30d9e88239f", "start_type": "ATG", "rbs_motif": "AGGAG/GGAGG", "genes": [], "truncated": "5-prime", "pseudogene": { "start": 650789, "stop": 651608, "observations": { "insertion": [ 651569 ], "stop": [ 651094 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E491E9D", "query_cov": 0.8864864864864865, "subject_cov": 0.4039408866995074, "identity": 0.9570000000000001, "score": 318.0, "evalue": 3.88e-106, "gene_start": 22, "gene_end": 185, "reference_start": 8, "reference_end": 171, "reference_sequence": "MGNIYYNLGFYINGGIIGVVIYYIFNYVNYSNGNGGGLFDGQKNKNYSMPNEERYLQSFNAKTIVKNNIQLKNKNYQYGNLSIFEYPFALTKNNEPIYFLGEEGRKLLNLEFLKRGFYGPEINGLNHVYINKKIPNVSNIKDVNGVYLPQAFDIFIQINHFISGGIKQVQSWSLQKRVELVFNTLFHEYAHHIDNTYNRSIRENDEFANNDLSYKLATPELNKIINKFNGNNNKFLTEFRKTLNYDGSMQNDEYLVNKELFNPKNKEIPVYSKFSQYDLFKLANLALTNKEVEKYNILNNKNFYFNNSTNNTVYYANSTNIDQLKYLFSFEELFARELVKLSYSAHENFQMFDGHLTLDDSLNFLYYKLKNDDIFLSSIGDDTLKMIANYYNKYVIYSPIEYLMMN" }, "paralog": false, "identity": 0.9852941176470589, "score": 549.0, "evalue": 2.03e-199, "description": "Internal stop codon at 651094. Frameshift due to insertion around 651569.", "psc": { "uniref90_id": "UniRef90_UPI001E491E9D", "product": "MYPU_1760 family metalloprotease", "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E491E9D", "UniRef:UniRef50_UPI001E491E9D" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E491E9D", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E491E9D" ], "product": "MYPU_1760 family metalloprotease" } }, "id": "GLJLFKCOHC_1114", "locus": "GCA013368035_01115" }, { "type": "cds", "sequence": "contig_1", "start": 651719, "stop": 652171, "strand": "+", "frame": 2, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "db_xrefs": [ "COG:COG0742", "COG:J", "EC:2.1.1.171", "GO:0031167", "GO:0052913", "KEGG:K08316", "SO:0001217", "UniRef:UniRef50_A0A077L643", "UniRef:UniRef90_A0A454CAI7" ], "nt": "ATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAGTCAATAACAAGACCAACAATAGATAGAGCGAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGGGGCGCAAAATTTGCGGTATGTGTTGATAAAAATGCCGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGATTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTGCTAGTTGAGTGTTTAAACAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGA", "aa": "MLRIIAGKYRSRILKQPSKSITRPTIDRAREAIFSSIQFNIENKKVLDLFSGSGAFCLEALSRGAKFAVCVDKNAEAIKIIKENQNSLKEPNLKIFNIDALNFLNNNVEVFDYIYLDPPFVEKKLLVECLNKIIDKKCIDKSGTMIIETD", "aa_hexdigest": "b71f3fdbd296b995342c242285c41994", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAI7", "query_cov": 1.0, "subject_cov": 0.8152173913043478, "identity": 0.993, "score": 292.0, "evalue": 1.81e-99, "valid": true, "gene": "rsmD", "product": "16S rRNA (guanine(966)-N(2))-methyltransferase RsmD", "ec_ids": [ "2.1.1.171" ], "uniref50_id": "UniRef50_A0A077L643", "cog_id": "COG0742", "cog_category": "J", "kegg_orthology_id": "K08316", "go_ids": [ "GO:0031167", "GO:0052913" ] }, "pscc": { "uniref50_id": "UniRef50_A0A077L643", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L643" ], "product": "N6-adenine-specific methylase" }, "genes": [ "rsmD" ], "id": "GLJLFKCOHC_1115", "locus": "GCA013368035_01116" }, { "type": "cds", "sequence": "contig_1", "start": 652325, "stop": 652948, "strand": "+", "frame": 2, "gene": "gmk", "product": "guanylate kinase", "db_xrefs": [ "COG:COG0194", "COG:F", "EC:2.7.4.8", "GO:0004385", "GO:0006163", "RefSeq:WP_175949974.1", "SO:0001217", "UniParc:UPI00158ED231", "UniRef:UniRef100_UPI00158ED231", "UniRef:UniRef50_Q98PN5", "UniRef:UniRef90_D1J8S4" ], "nt": "ATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTGTCAGTATCAATGACAACGAGATTACCACGAGAAGGAGAAATTGATGGTGTACACTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGAGCTAAACAAATTTTAGAATATTATAAAAAACGTAACAGATTGGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGCTATTCTACAATATTTCATCATGTGATAGTTAATGATGATTTAAATGTTGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAA", "aa": "MSKKLIIFTGPSGVGKGTIEKPLFEDKSLKLKLSVSMTTRLPREGEIDGVHYYFVSKDTFNSCLAENKLIEYSMHFDNYYGTLYSEIERISEQGKIPFLEIETNGAKQILEYYKKRNRLDEICSIFLMPPSYGELRKRIEGRNTETEDLIIKRMKKAKEEIGYSTIFHHVIVNDDLNVALEEIKEIIRSEFSDIINKKEVASEGRKA", "aa_hexdigest": "d65482e6a2272d312c2cae73cd9d02d1", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00158ED231", "ncbi_nrp_id": "WP_175949974.1", "uniref100_id": "UniRef100_UPI00158ED231", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158ED231", "RefSeq:WP_175949974.1", "UniRef:UniRef100_UPI00158ED231" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158ED231", "uniref90_id": "UniRef90_D1J8S4" }, "psc": { "uniref90_id": "UniRef90_D1J8S4", "gene": "gmk", "product": "guanylate kinase", "ec_ids": [ "2.7.4.8" ], "uniref50_id": "UniRef50_Q98PN5", "cog_id": "COG0194", "cog_category": "F", "go_ids": [ "GO:0004385", "GO:0006163" ] }, "pscc": { "uniref50_id": "UniRef50_Q98PN5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98PN5" ], "product": "Guanylate kinase" }, "genes": [ "gmk" ], "id": "GLJLFKCOHC_1116", "locus": "GCA013368035_01117" }, { "type": "cds", "sequence": "contig_1", "start": 653042, "stop": 653185, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGCTGCTTTGTGTGATGGTATGGGCGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAACAATATTTCCTAGACACATTTCCTCAAAAGTTGGAATGTGTTGATAAAAAAAACGTTAGCAAATGA", "aa": "MAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECVDKKNVSK", "aa_hexdigest": "024db3a0c3f3040a62103268956aa3b6", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5038.733300000002, "isoelectric_point": 7.717677879333496 }, "id": "GLJLFKCOHC_1117", "locus": "GCA013368035_01118" }, { "type": "cds", "sequence": "contig_1", "start": 653270, "stop": 653707, "strand": "+", "frame": 2, "gene": "pTC1", "product": "Protein phosphatase", "db_xrefs": [ "COG:COG0631", "COG:T", "EC:3.1.3.16", "KEGG:K20074", "SO:0001217", "UniRef:UniRef50_D1J8S5", "UniRef:UniRef90_D1J8S5" ], "nt": "ATGGTTGTTGCCCTCATTTTTAATGCAAATGGTTTAGCTTATGTCTTTAATATTGGCGATTCACGCTTGTATGCATACAATGGATTACTTTATCAAATCACAGAAGATCAAAATTATTTATATCAGTTAATGAGAGAATTTAATTTAACATACGAAGAAGCAGCATTAGATCCTAATTCATACAAACTTATAAGTTGTCTAGGACCAAATAAAAAAACCAATTGTCAATCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAATAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAA", "aa": "MVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNNSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE", "aa_hexdigest": "047451719cc46a0f53df156f44ee6d6d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "pTC1" ], "truncated": "5-prime", "pseudogene": { "start": 652970, "stop": 653707, "observations": { "mutation": [ 653036, 653183 ], "stop": [ 653036, 653183 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S5", "query_cov": 1.0, "subject_cov": 0.5776892430278885, "identity": 0.993, "score": 283.0, "evalue": 4.61e-95, "gene_start": 1, "gene_end": 145, "reference_start": 107, "reference_end": 251, "reference_sequence": "MVDFCIASDQGKFRKENQDRAIVVNGEYWTLAALCDGMGGHVGGSQCSTLSTNSIKQYFLDTFPQKLECSDKKNVSKWFNNAISFIKEALVNYAKEYTEFEDMGTTMVVALIFNANGLAYVFNIGDSRLYAYNGLLYQITEDQNYLYQLMREFNLTYEEAALDPNSYKLISCLGPNKKTNCQSFFISQKSAVKYYLLTSDGLHDYVSKPIIETVLQTNKSLKDKLNLLIKYAKKNLSKDNITGILVGLKNE" }, "paralog": false, "identity": 0.9836734693877551, "score": 480.0, "evalue": 3.84e-175, "description": "Internal stop codon at 653036, 653183. Nonsense mutation around 653036, 653183.", "psc": { "uniref90_id": "UniRef90_D1J8S5", "gene": "PTC1", "product": "Protein phosphatase", "ec_ids": [ "3.1.3.16" ], "uniref50_id": "UniRef50_D1J8S5", "cog_id": "COG0631", "cog_category": "T", "kegg_orthology_id": "K20074", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S5", "EC:3.1.3.16", "UniRef:UniRef50_D1J8S5", "COG:COG0631", "COG:T", "KEGG:K20074" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S5" ], "product": "Protein phosphatase" } }, "id": "GLJLFKCOHC_1118", "locus": "GCA013368035_01119" }, { "type": "cds", "sequence": "contig_1", "start": 653700, "stop": 653951, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00069.31", "PFAM:PF07714.23" ], "nt": "ATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTCGAATTAATTGGGACTGGAGGATTTGGTGAAGTTTATTCTGCTACATATAAAAAAGATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGTTGGACAGAGCAGAGAACGAGATTCATTAACGAAATAAATATATTAAAGGAAATTCATAGTTCTAACGTAGTAAAAATATTGGGCTGA", "aa": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKDNKRVAIKILNCIDDSRWTEQRTRFINEINILKEIHSSNVVKILG", "aa_hexdigest": "4c3a0d66d030114c3b31d8bcfb015a2e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00069.31", "name": "Pkinase", "length": 65, "aa_cov": 0.7831325301204819, "hmm_cov": 0.9692307692307692, "evalue": 1.5308971620158936e-13, "score": 46.0963134765625, "start": 18, "stop": 82 }, { "id": "PF07714.23", "name": "PK_Tyr_Ser-Thr", "length": 67, "aa_cov": 0.7590361445783133, "hmm_cov": 0.9552238805970149, "evalue": 1.230250618389967e-11, "score": 39.75590515136719, "start": 21, "stop": 83 } ], "seq_stats": { "molecular_weight": 9614.046599999996, "isoelectric_point": 9.130608940124514 }, "id": "GLJLFKCOHC_1119", "locus": "GCA013368035_01120" }, { "type": "cds", "sequence": "contig_1", "start": 653982, "stop": 654716, "strand": "+", "frame": 3, "gene": "sPS1", "product": "mitogen-activated protein kinase kinase", "db_xrefs": [ "COG:COG0515", "COG:T", "SO:0001217", "UniRef:UniRef50_D1J8S6", "UniRef:UniRef90_D1J8S6" ], "nt": "ATGGAGTTAATTAAAGGCCAAGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATCGATCCTGATACTGCAATTATGTATGCAAAGCAAATATGTCAGGGTTTGATTGAAATCCATGCTGCCAAAATAGTTCATAGAGATTTAAAACCAAGCAATATATTGATTGATGAAGAAACAAACAGCTTAAAATTAATTGATTTTGGTATTTCAATAGGTGAATCAACTCAACGTGTAACTAGAAATTATAAAGCTGTCGGTTCTGTTCAATATATGGCGCCTGAATTATTGCGGGGAATAGCTGATCCTTCTCCAAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGAAAGGTTCCTTTCAATAATCATGATGATATTAACCAAATCATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCACAAGCATTAGAAAATATTGTAATTCGTTGCACTGCAAAATCTGCAGAGCAAAGATATACTGATTGCATCGAACTAATGCATGATTTAGAGACATGTTTAGATTCAAAAAGAGTAAACGAAAAAAGATTGGAATTAAATCCAAGTAAGTCAAAAATGAACGCCAAAAGAATCTTTAATAGTAAATTATTCAATATTATTTTTTTCACAACAGGAGGCTTTTTAGTAGCCTTTATTGTGATATTCGTGGTTTTATTTTTAAAGGGGATATTGTAG", "aa": "MELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNILIDEETNSLKLIDFGISIGESTQRVTRNYKAVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCIELMHDLETCLDSKRVNEKRLELNPSKSKMNAKRIFNSKLFNIIFFTTGGFLVAFIVIFVVLFLKGIL", "aa_hexdigest": "5ba38050d7f824aa18535bb29c3e3901", "start_type": "ATG", "rbs_motif": null, "genes": [ "sPS1" ], "truncated": "5-prime", "pseudogene": { "start": 653700, "stop": 654716, "observations": { "mutation": [ 653949 ], "stop": [ 653949 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S6", "query_cov": 1.0, "subject_cov": 0.7218934911242604, "identity": 0.992, "score": 466.0, "evalue": 3.69e-164, "gene_start": 1, "gene_end": 244, "reference_start": 95, "reference_end": 338, "reference_sequence": "MNKIKELPGMFVLNKYFTDFELIGTGGFGEVYSATYKKDNKRVAIKILNCIDDSRWTEQRTRFINEINILKEIHSSNVVKILGWHYKGNEVFYVMELIKGQGLKTIIAKNKKIDPDTAIMYAKQICQGLIEIHAAKIVHRDLKPSNILIDEETNSLKLIDFGISIGESTQRVTRNYKAVGSVQYMAPELLRGIADPSPRSDIYAFGIILFEMLEGKVPFNNHDDINQIMMMHINNELPRMSDVNVIIPQALENIVIRCTAKSAEQRYTDCVELMHDLETCLDSKRVNEKRLELNPSKSKMKAKRIFNSKLFNIIFFTTGGFLVAFIVIFVVLFLKGIL" }, "paralog": false, "identity": 0.9911242603550295, "score": 665.0, "evalue": 3.58e-245, "description": "Internal stop codon at 653949. Nonsense mutation around 653949.", "psc": { "uniref90_id": "UniRef90_D1J8S6", "gene": "SPS1", "product": "mitogen-activated protein kinase kinase", "uniref50_id": "UniRef50_D1J8S6", "cog_id": "COG0515", "cog_category": "T", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S6", "UniRef:UniRef50_D1J8S6", "COG:COG0515", "COG:T" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S6" ], "product": "mitogen-activated protein kinase kinase" } }, "id": "GLJLFKCOHC_1120", "locus": "GCA013368035_01121" }, { "type": "cds", "sequence": "contig_1", "start": 654717, "stop": 655568, "strand": "+", "frame": 3, "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "db_xrefs": [ "COG:COG1162", "COG:J", "EC:3.1.3.100", "EC:3.6.1.-", "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022", "KEGG:K06949", "RefSeq:WP_044329804.1", "SO:0001217", "UniParc:UPI0005C96051", "UniRef:UniRef100_UPI0005C96051", "UniRef:UniRef50_D1J8S7", "UniRef:UniRef90_D1J8S7" ], "nt": "ATGATAGAAGGTAAAATAGTTAAATCAATTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGTGGCAAACTTCGCTTGCTTGATATAGTGCCAATTGTGGGCGACAACGTTTTGTTTAAGAAAGACGAATTCATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCGTTGGTTGAACCTGAATTTAGTTCATTGCTTGTTGATAAATTTTTGATTATTATTGAAAGTAAGAATGTCAAACCTATTATCGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATAGCTTTTTATAAAGATCAAGGCTATGAAGTTTTTGAAATTAATTACGAAACAGGCCAAGGATTTGAAGGCTTAGGAGAAATATTTAAACATAAGACAAGCTTCTTTGTTGGTCAAACAGGTGTAGGAAAAACTACCTTGATAAATTATTTGGCAAAAACAAATTTTGAAACGCAAGCAATTTCTAAAGCCTTAAACCGAGGAAAACATACAACAAGAGAAGTTAGTTTGATTGATTTTAACGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCAATAGAATTTGATTTAACAATTGAAGAACTACCGATTGCTTTTAAATCGTTTAGAGAGGCTGCAAGTAAATGCAAATTTAGAACAAGCTGTAAACATTATTTAGAATCCGTTGAGGATTGTGAAGTTAAAAAATTAGTTTTACAAGGTAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATCAAATAG", "aa": "MIEGKIVKSIAGFYDIKSFEDKQIYRVRGGGKLRLLDIVPIVGDNVLFKKDEFIYQILGRKNFFIRPKIANVDQAIVVMSLVEPEFSSLLVDKFLIIIESKNVKPIIVLTKKDLTSDSKIAFYKDQGYEVFEINYETGQGFEGLGEIFKHKTSFFVGQTGVGKTTLINYLAKTNFETQAISKALNRGKHTTREVSLIDFNGGEIIDTPGFSSIEFDLTIEELPIAFKSFREAASKCKFRTSCKHYLESVEDCEVKKLVLQGKIKQERYDNYISFLKRLTNPIK", "aa_hexdigest": "900405c20e342e4b1bdb8634cdb660fc", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005C96051", "ncbi_nrp_id": "WP_044329804.1", "uniref100_id": "UniRef100_UPI0005C96051", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C96051", "RefSeq:WP_044329804.1", "UniRef:UniRef100_UPI0005C96051" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C96051", "uniref90_id": "UniRef90_D1J8S7" }, "psc": { "uniref90_id": "UniRef90_D1J8S7", "gene": "rsgA", "product": "ribosome small subunit-dependent GTPase A", "ec_ids": [ "3.1.3.100", "3.6.1.-" ], "uniref50_id": "UniRef50_D1J8S7", "cog_id": "COG1162", "cog_category": "J", "kegg_orthology_id": "K06949", "go_ids": [ "GO:0003924", "GO:0005525", "GO:0006412", "GO:0043022" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S7" ], "product": "Small ribosomal subunit biogenesis GTPase RsgA" }, "genes": [ "rsgA" ], "id": "GLJLFKCOHC_1121", "locus": "GCA013368035_01122" }, { "type": "cds", "sequence": "contig_1", "start": 655682, "stop": 656224, "strand": "+", "frame": 2, "gene": "rpe", "product": "Ribulose-phosphate 3-epimerase", "db_xrefs": [ "COG:COG0036", "COG:G", "SO:0001217", "UniRef:UniRef50_A0A448ZXM9", "UniRef:UniRef90_A0A454CAB1" ], "nt": "ATGGATGGCATTTTTGTAAACAATACTGCTTTAACATTTCAAGAAATCAAACAAGTTAAAGAAAATTGCAAGAAACATACAATGGACATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCATTTGAAAATAGTGATCTTGAAAAGATGATTAAACAATGCCAAAAAGATAACGTTAAACTTGGCTTAGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGTTTTGGTGGCCAAAAATTTATTGAAAATTCATTTAATAAAATAAAGGAATTAGCAGACATTCGTAAAGCTTTAAATTTAAACTTTATTATTGAAGTTGATGGCGGAGTTAAAGACCAAAATATTGCTAAATGTTTTGAATGTGGTGTTGATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATCGAACAACTTTTAAAATAA", "aa": "MDGIFVNNTALTFQEIKQVKENCKKHTMDIHLMVKDVFKYYEMYKNIGDILTFHYEAFENSDLEKMIKQCQKDNVKLGLAIKPNTQVEEIRQYLKHCSLVLIMSVEPGFGGQKFIENSFNKIKELADIRKALNLNFIIEVDGGVKDQNIAKCFECGVDLAVVGSYLVKNFSKETIEQLLK", "aa_hexdigest": "2d4ca67afda99318b755150498351b60", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAB1", "query_cov": 1.0, "subject_cov": 0.8372093023255814, "identity": 0.983, "score": 359.0, "evalue": 5.46e-125, "valid": true, "gene": "Rpe", "product": "Ribulose-phosphate 3-epimerase", "uniref50_id": "UniRef50_A0A448ZXM9", "cog_id": "COG0036", "cog_category": "G" }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXM9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXM9" ], "product": "D-ribulose-5-phosphate 3 epimerase" }, "genes": [ "rpe" ], "id": "GLJLFKCOHC_1122", "locus": "GCA013368035_01123" }, { "type": "cds", "sequence": "contig_1", "start": 656230, "stop": 657099, "strand": "+", "frame": 1, "gene": null, "product": "Lipoprotein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9", "UniRef:UniRef90_D1J8S9" ], "nt": "ATGAAAAAAGCAAATAAAATATTACTAGCTACATTTGCAACTACAATTTTAGCATTGCCTCTTGCCACAATTTCTTGCAAAGTCGATCAAGTTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCTGCTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTAAAAACCCGTTTAGCAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTATTGAAATTCAATTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAACGAATTATTAAAATATTATAAATTAATGGCCTTGGTGCAAGATAAGTCTGAAGTAAACAAATACGCAGATTTAATCAAAAAATATTCAAAAAAATTTTCTGATGTTTTGAAGGATAATAGTTCAATATTAAATGATATTTACAATCAAGTTACAAATTTAATTCCTAGAATTAATTTGCTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATCCACTTTTTTGAATAATAATGGAATACTATTCAATTTGGATTTTATATTGAATAACATTGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAAAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTTTATAAAAATTATGAACTAGTTGAAGATTTTAATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGCAAATAATTATTTGGTTAATTGA", "aa": "MKKANKILLATFATTILALPLATISCKVDQVKEKEKLINNSSLLNKENKKILLNELNILKTRLANSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALVQDKSEVNKYADLIKKYSKKFSDVLKDNSSILNDIYNQVTNLIPRINLLKNSYIFSLNINNKKSTFLNNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFANNYLVN", "aa_hexdigest": "a8f02801994bb616fdbd735af6c165c3", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 656230, "stop": 657351, "observations": { "mutation": [ 657097 ], "stop": [ 657097 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8S9", "query_cov": 1.0, "subject_cov": 0.774798927613941, "identity": 0.965, "score": 518.0, "evalue": 2.21e-183, "gene_start": 1, "gene_end": 289, "reference_start": 1, "reference_end": 289, "reference_sequence": "MKKANKILLSTFATTILALPFATISCKVDQVKEKEKLINNSSQLNKENKKILLNELNILKTRLVNSKLNEKQKNEFIEIQLFLKLITLKELKISNELLKYYKLMALLQDKSEVNKYADLIKKYSKKFSDVLKDDSSILNDIYNQVANLIPRINLLKNSYIFSLNINNKKFTFLSNNGILFNLDFILNNIENKENKYSIYYNTKKYELNLEIKNIEFNSNIKTALDFKNQDFLTTNFLDYDKDLVCNEMLKNAMVYKNYELVEDFNENDKNIKFSKMFTSQDFVNNYLVNWLTNEPNICQIGEDKFLIYYHLPFSIENKKIVQKPSLYISNVVADSTIIKAIASRNVYLLKNLFLLLPKENYNENLKVLNNLMK" }, "paralog": false, "identity": 0.967828418230563, "score": 706.0, "evalue": 2.73e-260, "description": "Internal stop codon at 657097. Nonsense mutation around 657097.", "psc": { "uniref90_id": "UniRef90_D1J8S9", "product": "Lipoprotein", "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8S9", "UniRef:UniRef50_D1J8S9" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8S9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8S9" ], "product": "Lipoprotein" } }, "id": "GLJLFKCOHC_1123", "locus": "GCA013368035_01124" }, { "type": "cds", "sequence": "contig_1", "start": 657734, "stop": 658273, "strand": "+", "frame": 2, "gene": null, "product": "Lmp related protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8T0", "UniRef:UniRef50_UPI001939F067", "UniRef:UniRef90_D1J8T0" ], "nt": "ATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCATATTATGTGGAGGTTTAGCGGTGGCATCAATTGCTACAGTTGCTGCATACGCATGTAAATCAAGATCAGAATCAAAAATAGAAAAAGAAAATCTTTTGAATGAACTTAAAGAGGCATTAAATCAATTAAAACAATCGATTGCATCACCAGAAGCTAAAAATATTGAAAAATCAGTGGAAATCAATATCTTAAATAATGCTAATGTTTCAAAAGAATCCTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAACAAGGAATTTGAAAAATTTGAACAAATCAAAAACGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATCGAAATTAAATAACATATCGCAAAGCTCAAAAGAACAAGAAATTATAGACGCAATAAGCAAATTGTATGATTTAAAGAAATGA", "aa": "MYKKTNRKILISLAILCGGLAVASIATVAAYACKSRSESKIEKENLLNELKEALNQLKQSIASPEAKNIEKSVEINILNNANVSKESSIKEIQSKINQVKYAIDSLNKKIADRNNKEFEKFEQIKNDLQTYINRDLNVVIYNEITKKSKNEISKLNNISQSSKEQEIIDAISKLYDLKK", "aa_hexdigest": "9561300efca1c607a9493d8cb7764ace", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI001939F067", "query_cov": 1.0, "subject_cov": 0.8731707317073171, "identity": 0.8270000000000001, "score": 221.0, "evalue": 8.4e-71, "valid": false, "uniref50_id": "UniRef50_UPI001939F067" }, "pscc": { "uniref50_id": "UniRef50_UPI001939F067", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001939F067" ] }, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 657734, "stop": 658576, "observations": { "mutation": [ 658271 ], "stop": [ 658271 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8T0", "query_cov": 1.0, "subject_cov": 0.5507692307692308, "identity": 0.86, "score": 226.0, "evalue": 3.82e-71, "gene_start": 1, "gene_end": 179, "reference_start": 1, "reference_end": 179, "reference_sequence": "MYKKTNRKILISLAILCGGLAVASIATVAAYAGKSKPESKIEKENLLNELKEALNQLKQLIASPEAKNIEKSMKINILNNANVSKDSSIKEIQSKINQVKYAINSLNKKIADRNKKEFEKFEQIKNDLQTYINRDLNVVIYNEITKKSKNEILKLNNISQSSKEQEIIDAISKLYDLKKWILAQKLLIDNSINEQKRQLKSLIKIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTTSELFRERILLHKCINETLLFEETMFKEKETKINELKKQSETIFEHIGKDPNNSIFDYNSQFYRNIITKIDSLNTNEKSLTLNKKI" }, "paralog": false, "identity": 0.8964285714285715, "score": 427.0, "evalue": 1.95e-152, "description": "Internal stop codon at 658271. Nonsense mutation around 658271.", "psc": { "uniref90_id": "UniRef90_D1J8T0", "product": "Lmp related protein", "uniref50_id": "UniRef50_D1J8T0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8T0", "UniRef:UniRef50_D1J8T0" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8T0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8T0" ], "product": "Lmp related protein" } }, "id": "GLJLFKCOHC_1124", "locus": "GCA013368035_01125" }, { "type": "cds", "sequence": "contig_1", "start": 658361, "stop": 658840, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E", "UniRef:UniRef90_UPI001594B72E" ], "nt": "TTGTTATCAAACAAAATAGTTGAACGTGACCTAGAATTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAACAAAACTGCATCTGAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCTATTATTTGAAGAAACAATGTTTAAAGAAAAGGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACAATTTTTGAACATATTGGAAAAGACCCAAATAATAGCATATTCGATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACACTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGTAATGAAATAAATAATCTAGACAAATCATTTTCTAAGTTGGTTTCTGATACCCTTGAAGAATTTGAATATGAGGATGATCTTAAAGACCATATTGATGTTGCAAAAGATCCCAAAAATTGA", "aa": "MLSNKIVERDLEFKQEISKAEASLSQTNKTASELFRERILLHKFINETLLFEETMFKEKETKINELKKQSETIFEHIGKDPNNSIFDYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEEFEYEDDLKDHIDVAKDPKN", "aa_hexdigest": "6c077bb5ff211c0e66dcef40535d5ee8", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 658193, "stop": 658880, "observations": { "insertion": [ 658811 ], "mutation": [ 658271 ], "stop": [ 658271 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001594B72E", "query_cov": 0.9937106918238994, "subject_cov": 0.6899563318777293, "identity": 0.924, "score": 266.0, "evalue": 2.74e-88, "gene_start": 1, "gene_end": 158, "reference_start": 58, "reference_end": 215, "reference_sequence": "MKLNSISQSSKEQEIIDAISKLYDLKKWILAQKLLIDNSINEQKRQLKSLIEIANQILLSNKIVERDLEFKQEISKAEASLSQTNKTTSELFRERILLHKFINETLLLEEKMLKEKETKINELKKQSETIFEHIGKDPNNSIFYYNSQFYRNIITKIDSLNTNEKSLTLNKKIEDISNEINNLDKSFSKLVSDTLEKFEYEDDLKDHMMLQKIPKIELIFKNISDTLGQ" }, "paralog": false, "identity": 0.9517543859649122, "score": 407.0, "evalue": 1.21e-146, "description": "Internal stop codon at 658271. Frameshift due to insertion around 658811. Nonsense mutation around 658271.", "psc": { "uniref90_id": "UniRef90_UPI001594B72E", "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001594B72E", "UniRef:UniRef50_UPI001594B72E" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001594B72E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001594B72E" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 18728.76329999999, "isoelectric_point": 4.963203620910644 }, "id": "GLJLFKCOHC_1125", "locus": "GCA013368035_01126" }, { "type": "cds", "sequence": "contig_1", "start": 659123, "stop": 659350, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "ATGCAAGTAGACCAAATAACAGACGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTTTGAAAGCAAACTATTAAGTGCAATATTGTTGCCTACAATATTTAAAAAGCCATATGAAAATCTTTCTAAAATAGTAAAGCCAATTATAGATATGATACATAATAAAGACGAATATAGCATAGAGATTATTGGATGA", "aa": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIG", "aa_hexdigest": "798d9665491a9955e8927b63f637e1ad", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 659123, "stop": 659653, "observations": { "mutation": [ 659348 ], "stop": [ 659348 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 1.0, "score": 142.0, "evalue": 1.12e-41, "gene_start": 1, "gene_end": 75, "reference_start": 1, "reference_end": 75, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9829545454545454, "score": 329.0, "evalue": 9.14e-118, "description": "Internal stop codon at 659348. Nonsense mutation around 659348.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 8709.233199999995, "isoelectric_point": 5.721890449523926 }, "id": "GLJLFKCOHC_1126", "locus": "GCA013368035_01127" }, { "type": "cds", "sequence": "contig_1", "start": 659432, "stop": 659659, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6", "UniRef:UniRef90_UPI00193AA5C6" ], "nt": "TTGACACAAGAAAATTTAAAAAATAAATTAAAAGAATTTAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAAGCATCCGAAAAAGACATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAGTTCTATTATTAGAATAAAAAAAGAAATAAGATACCTTAAATATAAACTAGATTAA", "aa": "MTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD", "aa_hexdigest": "e543fbce8307ee00b337215e089de2fd", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 659132, "stop": 659659, "observations": { "mutation": [ 659348 ], "stop": [ 659348 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00193AA5C6", "query_cov": 1.0, "subject_cov": 0.42134831460674155, "identity": 0.987, "score": 137.0, "evalue": 1.06e-39, "gene_start": 1, "gene_end": 75, "reference_start": 104, "reference_end": 178, "reference_sequence": "MQVDQITDEMKTKIFDLLIKDLSNILKKDSFESKLLSAILLPTIFKKPYENLSKIVKPIIDMIHNKDEYSIEIIGWEKASQELSLDISNIKEAEKKALEIKAKLTQENLKNKLKEFKLVKENLGNLIKKASEKDIEVEEDTKFIYENSEEDENSTLYEVISSIIRIKKEIRYLKYKLD" }, "paralog": false, "identity": 0.9828571428571429, "score": 327.0, "evalue": 3.71e-117, "description": "Internal stop codon at 659348. Nonsense mutation around 659348.", "psc": { "uniref90_id": "UniRef90_UPI00193AA5C6", "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00193AA5C6", "UniRef:UniRef50_UPI00193AA5C6" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00193AA5C6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00193AA5C6" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9001.181399999992, "isoelectric_point": 5.288549995422362 }, "id": "GLJLFKCOHC_1127", "locus": "GCA013368035_01128" }, { "type": "cds", "sequence": "contig_1", "start": 660652, "stop": 661266, "strand": "+", "frame": 1, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "nt": "ATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTAGCTTTTAAAAATGGCGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTTTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATTACAGTTCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAAAACGCAGGAATTATTGCTGGTCTTGATGTTGTAAGAATTATTAACGAACCTACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCGTTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTAGCAACAGCAGGCGATAACCACTTGGGTGGTGATGACTGA", "aa": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDD", "aa_hexdigest": "8ddb54cc4cdd33d560f0071fd07de60e", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI002E1EEFC2", "query_cov": 0.9754901960784313, "subject_cov": 0.9660194174757282, "identity": 0.6729999999999999, "score": 264.0, "evalue": 4.31e-87, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A3D2IMT5" }, "pscc": { "uniref50_id": "UniRef50_A0A3D2IMT5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3D2IMT5" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "GLJLFKCOHC_1128", "locus": "GCA013368035_01129" }, { "type": "cds", "sequence": "contig_1", "start": 661288, "stop": 661563, "strand": "+", "frame": 1, "gene": "dnaK", "product": "Chaperone protein DnaK", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "nt": "ATGGTTGAACAAATTAAATCTAAATACAATTTTGATCCAACAACCGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACTCGTGCTACATTTGAATCAATGACTGAACATTTACTACAAAGAACAAAAAAACCATTATTAGATGTTTTAAGTGAAGCAAAATTAACATGA", "aa": "MVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTKKPLLDVLSEAKLT", "aa_hexdigest": "4e642841e4cb695772b40ef48d2c5583", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00387B89B0", "query_cov": 0.9340659340659341, "subject_cov": 0.8252427184466019, "identity": 0.5760000000000001, "score": 105.0, "evalue": 9.4e-28, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_A0A223M9D4" }, "pscc": { "uniref50_id": "UniRef50_A0A223M9D4", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A223M9D4" ], "product": "Chaperone protein DnaK" }, "genes": [ "dnaK" ], "id": "GLJLFKCOHC_1129", "locus": "GCA013368035_01130" }, { "type": "cds", "sequence": "contig_1", "start": 661579, "stop": 662439, "strand": "+", "frame": 1, "gene": "dnaK", "product": "molecular chaperone DnaK", "db_xrefs": [ "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662", "SO:0001217", "UniRef:UniRef50_Q49539", "UniRef:UniRef90_Q9ZEJ0" ], "nt": "GTGTTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAACTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCAGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGACGTTACTCCTTTAACATTAGGTATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATCGTTATCACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTCAATCTTGAAGGAATTGAACCTGCGCCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGATAAAAAGACAAACAAAGAACAAACAATCACCATCCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGATGCAGAAGCAAATAGAGAAGCTGATCAAAAGAAACGCCACGAAATTGAAGTTATTGTAAAAGCAGAACAATTAAGCAATGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAATCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGGAATAGATGAAATTAAAGAATTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAATGACAACCCTCAAACTAACTAA", "aa": "MLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKGIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN", "aa_hexdigest": "9518783dfb99dfe63c27dfd7ab350b0f", "start_type": "GTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI002A7481CD", "query_cov": 0.9230769230769231, "subject_cov": 0.88, "identity": 0.527, "score": 244.0, "evalue": 1.46e-76, "valid": false, "product": "Hsp70 family protein", "uniref50_id": "UniRef50_Q6L5N6" }, "genes": [ "dnaK" ], "truncated": "5-prime", "pseudogene": { "start": 661279, "stop": 662439, "observations": { "mutation": [ 661285, 661561 ], "stop": [ 661285, 661561 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_Q9ZEJ0", "query_cov": 0.9965034965034965, "subject_cov": 0.4789915966386555, "identity": 0.996, "score": 508.0, "evalue": 1.84e-176, "gene_start": 2, "gene_end": 286, "reference_start": 311, "reference_end": 595, "reference_sequence": "MAKEFILGIDLGTTNSVVSVIENGTPKILENPNGKRTTPSVVAFKNGETIIGESAKRQLESNKDSVASIKRLMGTSQTVHLNNKDYKPEEISAMILSYMKDYADKKLGQPVKKAVITVPAYFDNAQREATKNAGIIAGLDVVRIINEPTAAALAFGLNKDKNENQKILVFDLGGGTFDVSLLEMESGTFEVLATAGDNHLGGDDWDHEIVKWMVEQIKSKYNFDPTTDKMAMARLKEEAERAKITLSEQLIANISLPFLAMNENGPVNVELEITRATFESMTEHLLQRTRKPLLDVLSEAKLTWNDINEVLLVGGSTRMPAVQKLVAEVTNKKPNNSINPDEVVSVGAAIQGAILAGEIQDVLLLDVTPLTLGIVVEGDVVAPLIPRNTTIPVTKSQIFSTAVDNQTAVTIVITQGERQLARDNKILGQFNLEGIEPAPRGIPQIEVSFSIDVNGITKVTAKDKKTNKEQTITIQNTSSLSKEEVEKMVKDAEANREADQKKRHEIEVIVKAEQLSNDLEKTLKSEQAKNLGEPQKQELQKEIDEIKELINKKDIEQLEKKITEFEQKMAQAAEFLKKQQGNNNPNTNNDNPQTN" }, "paralog": false, "identity": 0.9896373056994818, "score": 726.0, "evalue": 1.05e-264, "description": "Internal stop codon at 661285, 661561. Nonsense mutation around 661285, 661561.", "psc": { "uniref90_id": "UniRef90_Q9ZEJ0", "gene": "dnaK", "product": "molecular chaperone DnaK", "uniref50_id": "UniRef50_Q49539", "cog_id": "COG0443", "cog_category": "O", "go_ids": [ "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_Q9ZEJ0", "UniRef:UniRef50_Q49539", "COG:COG0443", "COG:O", "GO:0005524", "GO:0006457", "GO:0016887", "GO:0051082", "GO:0140662" ] }, "pscc": { "uniref50_id": "UniRef50_Q49539", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q49539" ], "product": "Chaperone protein DnaK" } }, "id": "GLJLFKCOHC_1130", "locus": "GCA013368035_01131" }, { "type": "cds", "sequence": "contig_1", "start": 662661, "stop": 662963, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTAATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATAATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATCTGA", "aa": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAII", "aa_hexdigest": "13c2c510db0c8a1b34ed134faf46f4eb", "start_type": "ATG", "rbs_motif": "GGAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11355.716099999987, "isoelectric_point": 9.781288337707515 }, "id": "GLJLFKCOHC_1131", "locus": "GCA013368035_01132" }, { "type": "cds", "sequence": "contig_1", "start": 663006, "stop": 663419, "strand": "+", "frame": 3, "gene": null, "product": "ABC transporter permease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B", "UniRef:UniRef90_UPI001E50136B" ], "nt": "TTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGGCATTGGTTGCACATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATCATAATTTTTGCACCTATGGCAATAGGTGGCGGATTTATATTTGCCAACTCAACATCAACAAATGAAAATTTTGCCCATTATATTAATAGCAAATATAAATACCACAGAGCAGGAAATGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCTTGA", "aa": "MFAFVSSIIVYSQVSKGNMFGYLVLLALVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLA", "aa_hexdigest": "1250f1d1d8da8eb3e0a3ac646024dc7c", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 662706, "stop": 663547, "observations": { "deletion": [ 663537 ], "mutation": [ 662961, 663000, 663417, 663447, 663462 ], "stop": [ 662961, 663000, 663417, 663447, 663462 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E50136B", "query_cov": 1.0, "subject_cov": 0.46440677966101696, "identity": 0.985, "score": 259.0, "evalue": 5.31e-85, "gene_start": 1, "gene_end": 137, "reference_start": 116, "reference_end": 252, "reference_sequence": "MKTALNNYHNFLFKVTAKKKSTIIIPIILLLCSLILCFVFVGTKPAPRYFNVIIFAYTLVAILFTVLYGSLKSLNIFKDLEQDGIELIIFSKPISRKAIIWGKILSFNSLGLIWTLFAFVSSIIVYSQVSKGNMFGYLVLLSLVAHFLAYTIFGYIAALIAYKVNQKIAITVPIIIFAPMAIGGGFIFANSTSTNENFAHYINSKYKYHRAGNEVNSEVFYLNKNDDKYYLVPNGINNNKFSDVQNQYLNLAWKYSNSSANEWQKYSWLAMPYQFVDIFNIENQNIFSNLSSRQY" }, "paralog": false, "identity": 0.9785714285714285, "score": 524.0, "evalue": 6.12e-192, "description": "Internal stop codon at 662961, 663000, 663417, 663447, 663462. Frameshift due to deletion around 663537. Nonsense mutation around 662961, 663000, 663417, 663447, 663462.", "psc": { "uniref90_id": "UniRef90_UPI001E50136B", "product": "ABC transporter permease", "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E50136B", "UniRef:UniRef50_UPI001E50136B" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E50136B", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E50136B" ], "product": "ABC transporter permease" } }, "id": "GLJLFKCOHC_1132", "locus": "GCA013368035_01133" }, { "type": "cds", "sequence": "contig_1", "start": 663471, "stop": 663833, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGCCATATCAATTTGTTGATATATTCAACATTGAAAATCAAAATATTTTTAGTAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAACTAAGATTAATAAATACTATGTCCCAGGGATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCTTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGA", "aa": "MPYQFVDIFNIENQNIFSNLSSDSINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLK", "aa_hexdigest": "b6aa917564e8c4b1a5a800e5c70bec1f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 13912.351099999982, "isoelectric_point": 7.826155662536623 }, "id": "GLJLFKCOHC_1133", "locus": "GCA013368035_01134" }, { "type": "cds", "sequence": "contig_1", "start": 663840, "stop": 664427, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247", "UniRef:UniRef90_UPI001E5A3247" ], "nt": "ATGAGCCAAGTTTTAAAATATCATAAATTTACAGAAGCAGCCGAAGAATTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCACATGATATTAAAAAGTTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTACAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCGTTGCTATATTTTATCTATTTTAAAGATAATCAAGGTCAAGTTATTAAATCATTATTGAAATCTGAAAGTGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTGCAAGAAAATAAAATAACTAAAAATAAACAAGTTGTAGTTAGATATGAATTAATTCCAAGCAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGA", "aa": "MSQVLKYHKFTEAAEEFYSKVKNEISLKKLVKPHDIKKLLLETISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILI", "aa_hexdigest": "1714de7b6c4bf654923db67fee8a3efd", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "both", "pseudogene": { "start": 663540, "stop": 664493, "observations": { "mutation": [ 663831, 664425 ], "stop": [ 663831, 664425 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E5A3247", "query_cov": 1.0, "subject_cov": 0.5571428571428572, "identity": 0.985, "score": 369.0, "evalue": 8.5e-127, "gene_start": 1, "gene_end": 195, "reference_start": 134, "reference_end": 328, "reference_sequence": "MNDKNIHDLQCHINLLIYSTLKIKIFLVTFLRDNINNSLSNYLYYKNQENTIYKYKLNRNSDLLKLPTLENKTKINKYYVPGMLNLNSNIDNEVNNGVIYARKNASNFSASFEEDNFVYASPNNLVGKLKWKIMSQVLKYHKFIEAAEEFYSKVKNEISLKKLVKPYDIKKLLLEMISNEVNNTNSKFFNIDNVNLAVFDPHGVRDGRLQSETERKIYIATALLYFIYFKDNQGQVIKSLLKSESDKTTYAPSQIEIKVDGYIYEIGGYESYEPKQQVQENKITKNKQVVVRYELIPSNKNYLFQTVDDVYSIERDRKIVNKQFYILIWIVVAIILMMANRFLYLRKDYK" }, "paralog": false, "identity": 0.9810725552050473, "score": 612.0, "evalue": 4.25e-225, "description": "Internal stop codon at 663831, 664425. Nonsense mutation around 663831, 664425.", "psc": { "uniref90_id": "UniRef90_UPI001E5A3247", "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E5A3247", "UniRef:UniRef50_UPI001E5A3247" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E5A3247", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E5A3247" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 22894.838900000006, "isoelectric_point": 8.76139850616455 }, "id": "GLJLFKCOHC_1134", "locus": "GCA013368035_01135" }, { "type": "cds", "sequence": "contig_1", "start": 664493, "stop": 665197, "strand": "+", "frame": 2, "gene": "rbbA", "product": "ABC transporter ATP-binding protein", "db_xrefs": [ "COG:COG1131", "COG:JV", "RefSeq:WP_020002508.1", "SO:0001217", "UniParc:UPI000365E072", "UniRef:UniRef100_A0A454CAA1", "UniRef:UniRef50_A0A449B6T2", "UniRef:UniRef90_D1J8T4" ], "nt": "ATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGTGAAAATGGTGCAGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGTGGAATTACAAACTTAAACCCTAATTCTAAAAAAATTCTTGGTTATGTTCCAGAAAATGCAGTATTTCCTAAGGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCAGGAATAAATAAAACCGAAGTAAAGGCAAAAATCGAAAGTTATTTACAAAGGTTTGGAATTATTGAATTAAAAAACGAAAAACCTGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAAGCTTTAATTAACGAGCCTAAATTATTGATTTTAGATGAACCAGCTGCTAATTTAGACCCTACTGCAAGATTTGAATTGTTTTCAATACTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCGTTGACATTAATTCATGGCGGAAAGATTTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAG", "aa": "MDNHILELKNVVKIYNKSDRGIHNISFAVESGNFHAFIGENGAGKTTTIKTIIGSYINFKGEILIGGITNLNPNSKKILGYVPENAVFPKELTTYEYLKALALLSGINKTEVKAKIESYLQRFGIIELKNEKPANFSSGQKKKVLLIQALINEPKLLILDEPAANLDPTARFELFSILHELNEKGTTIFISSHVLSEIDKYANSLTLIHGGKILYSGPKYDSLEKIFYEKVIKN", "aa_hexdigest": "cbe68b00230783e80869ce26faa8a7ac", "start_type": "ATG", "rbs_motif": "AGGA", "ups": { "uniparc_id": "UPI000365E072", "ncbi_nrp_id": "WP_020002508.1", "uniref100_id": "UniRef100_A0A454CAA1", "db_xrefs": [ "SO:0001217", "UniParc:UPI000365E072", "RefSeq:WP_020002508.1", "UniRef:UniRef100_A0A454CAA1" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CAA1", "uniref90_id": "UniRef90_D1J8T4" }, "psc": { "uniref90_id": "UniRef90_D1J8T4", "gene": "RbbA", "product": "ABC transporter ATP-binding protein", "uniref50_id": "UniRef50_A0A449B6T2", "cog_id": "COG1131", "cog_category": "JV" }, "pscc": { "uniref50_id": "UniRef50_A0A449B6T2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A449B6T2" ], "product": "ABC transporter ATP-binding protein" }, "genes": [ "rbbA" ], "id": "GLJLFKCOHC_1135", "locus": "GCA013368035_01136" }, { "type": "cds", "sequence": "contig_1", "start": 665175, "stop": 665567, "strand": "+", "frame": 3, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35", "UniRef:UniRef90_UPI000A573B35" ], "nt": "ATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCATTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATCGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATTCAAGCAAAAATCAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGACATTTTTGTACAAAATCAAAATAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTTCCTATTTATAATAGAGCCTCAGATAATCCGGGTAGCGAACTTTATGGATTTATAAGACAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGA", "aa": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRN", "aa_hexdigest": "bf92365f4129e4aec179e2b3aa3b5ef9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 665175, "stop": 665700, "observations": { "insertion": [ 665649 ], "mutation": [ 665565, 665571 ], "stop": [ 665565, 665571 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A573B35", "query_cov": 1.0, "subject_cov": 0.7471264367816092, "identity": 1.0, "score": 243.0, "evalue": 9.04e-81, "gene_start": 1, "gene_end": 130, "reference_start": 1, "reference_end": 130, "reference_sequence": "MKKLLKISLSLPLVIATIPLVASSCKQTNIEIKKPDDKKETANQIQAKINKEFQKNKVVQKLLSSFIENEKEQDIFVQNQNNILPNVYSELKFSLLYFPIYNRASDNPGSELYGFIRQSREIIENTLQRNWYWYLKQIGNFVYSINPYNDRYIETSQTKKQFNLPKKTLVFYIK" }, "paralog": false, "identity": 0.9770114942528736, "score": 328.0, "evalue": 1.23e-116, "description": "Internal stop codon at 665565, 665571. Frameshift due to insertion around 665649. Nonsense mutation around 665565, 665571.", "psc": { "uniref90_id": "UniRef90_UPI000A573B35", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A573B35", "UniRef:UniRef50_UPI000A573B35" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A573B35", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A573B35" ], "product": "aromatic motif membrane protein" } }, "id": "GLJLFKCOHC_1136", "locus": "GCA013368035_01137" }, { "type": "cds", "sequence": "contig_1", "start": 665709, "stop": 666104, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE", "UniRef:UniRef90_UPI000A5C6EDE" ], "nt": "ATGCCTAAGGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTCGCTAAAAAAATTAATGAAAGTATTGATTCTAAAGTTCAAAACCAATTTAGCGATTATTTAGAAGATAGAATTGATGAATCATCGAAGTTAAAAATAGATACAAATTCAGAAATAACCAATTTTTTAAGAAACAAAATTAACTTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAATAATAAGAACAAATTTGAAATTAGTAATCAATATCAAATTAATAATGAAATTTCTAATAAAATATTTAATCTATTAAATACAAAAGGATCTTCATTTGACAACTATACTTCAAAGAAAATCTTTTACGTAGCATATGACTACAAAACTTGA", "aa": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDTNSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNQYQINNEISNKIFNLLNTKGSSFDNYTSKKIFYVAYDYKT", "aa_hexdigest": "d09a12e9ee2629d5585e71541e8d000d", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "3-prime", "pseudogene": { "start": 665709, "stop": 666407, "observations": { "mutation": [ 666102 ], "stop": [ 666102 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "query_cov": 1.0, "subject_cov": 0.5282258064516129, "identity": 0.9620000000000001, "score": 236.0, "evalue": 8.44e-77, "gene_start": 1, "gene_end": 131, "reference_start": 1, "reference_end": 131, "reference_sequence": "MPKDIIELPYNPISSDELKEIVAKKINESIDSKVQNQFSDYLEDRIDESSKLKIDINSEITNFLRNKINLNDAKKLIENYLNNKNKFEISNEYQINNEISKKIFNLLNTKGSSFDNYSSKKIFYVAYDHKTWIRVIVYTENNVKKISINPDVYILNQSNSYEQSKEILKKLNKQIFIERLNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE" }, "paralog": false, "identity": 0.9741379310344828, "score": 445.0, "evalue": 1.01e-161, "description": "Internal stop codon at 666102. Nonsense mutation around 666102.", "psc": { "uniref90_id": "UniRef90_UPI000A5C6EDE", "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI000A5C6EDE", "UniRef:UniRef50_UPI000A5C6EDE" ] }, "pscc": { "uniref50_id": "UniRef50_UPI000A5C6EDE", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000A5C6EDE" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 15390.065999999979, "isoelectric_point": 5.557341575622559 }, "id": "GLJLFKCOHC_1137", "locus": "GCA013368035_01138" }, { "type": "cds", "sequence": "contig_1", "start": 666246, "stop": 666455, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAATTCAATTATTGATTCTTTAATTGAAAATATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAA", "aa": "MNSIIDSLIENINEKDFNIKNFFEKNNDKKFFELFQKFSYNKYLIEAMQKINKEKDLIYRYSWETVDYE", "aa_hexdigest": "bcfa9f81827cb6780bcbbaa8e8746bb1", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8619.6525, "isoelectric_point": 5.142019081115721 }, "id": "GLJLFKCOHC_1138", "locus": "GCA013368035_01139" }, { "type": "cds", "sequence": "contig_1", "start": 666448, "stop": 666753, "strand": "+", "frame": 1, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1", "UniRef:UniRef90_UPI00241181B1" ], "nt": "ATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACCGCTATATCGTGCGTTGATAAAAGTGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGGGATGTTGTAAACAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAACGACTTATTGAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTGGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGA", "aa": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKK", "aa_hexdigest": "670d981eccc8d6508d2c5805232c3907", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 666448, "stop": 667056, "observations": { "mutation": [ 666751, 666823, 666874 ], "stop": [ 666751, 666823, 666874 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00241181B1", "query_cov": 1.0, "subject_cov": 0.4855769230769231, "identity": 1.0, "score": 185.0, "evalue": 1.13e-57, "gene_start": 1, "gene_end": 101, "reference_start": 1, "reference_end": 101, "reference_sequence": "MNKRNLLNFLFVPTLILAPLTAISCVDKSEKSDLNALATKIGDVVNKKENPAIEVTKKIINDLLKQVYRDNDVEKINFLDQQQNKEYQKNIFLKIKELSKKWNSKLNNTEKENIANQLNQIYEKNWYIVLTNINKFNLRFYEWLLFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLRELKEGDESGELSNMNMLYLKKR" }, "paralog": false, "identity": 0.9801980198019802, "score": 387.0, "evalue": 5.19e-140, "description": "Internal stop codon at 666751, 666823, 666874. Nonsense mutation around 666751, 666823, 666874.", "psc": { "uniref90_id": "UniRef90_UPI00241181B1", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00241181B1", "UniRef:UniRef50_UPI00241181B1" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00241181B1", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00241181B1" ], "product": "aromatic motif membrane protein" } }, "id": "GLJLFKCOHC_1139", "locus": "GCA013368035_01140" }, { "type": "cds", "sequence": "contig_1", "start": 666880, "stop": 667143, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTTTGATAAGCCTGAATTTTTAAGTCAAATTAGGGCAGATGGAAAGCATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAGAAACCAGATAATGTTCCGATTATTAATAATTATTTAAAGGAATTAAAAGAAGGCGATGAATCAGGGGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTGACAAAACCAAAAATCAAACTAACTTGTTTTGTTTGA", "aa": "MFDKPEFLSQIRADGKHSKEYLNSLKSEKKPDNVPIINNYLKELKEGDESGELSNMNMLYLKKDSMIFRFRVVDTLTKPKIKLTCFV", "aa_hexdigest": "c03b67fc41a90cbb416fdcf9376ff96d", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10187.76769999999, "isoelectric_point": 9.01450138092041 }, "id": "GLJLFKCOHC_1140", "locus": "GCA013368035_01141" }, { "type": "cds", "sequence": "contig_1", "start": 667177, "stop": 667341, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTTCAGATATTGTTCATAGCGCTTATATTCACGGTGTTCAAGAAGGATTCAATAGATTTGAAAATGATATGGCAAACCGTTATGGCAAACCTGCCAAAATGATCATGGGAATTTTCGATGATAATGCTTCTAGCGCCAACGGAGGGGAAGAAAATGAATAA", "aa": "MISDIVHSAYIHGVQEGFNRFENDMANRYGKPAKMIMGIFDDNASSANGGEENE", "aa_hexdigest": "b54393e84d362da2668720e0d9f5e886", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5979.497800000001, "isoelectric_point": 4.690774345397949 }, "id": "GLJLFKCOHC_1141", "locus": "GCA013368035_01142" }, { "type": "cds", "sequence": "contig_1", "start": 667334, "stop": 667492, "strand": "+", "frame": 2, "gene": null, "product": "aromatic motif membrane protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388", "UniRef:UniRef90_UPI001E405388" ], "nt": "ATGAATAAAAAAATTTTGATGACTCTATCATTAAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATCGAGGGCAATGAAAATGCAAACGAAAGAGAATGA", "aa": "MNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANERE", "aa_hexdigest": "b64cf36178635a37a8549461ed4726ef", "start_type": "ATG", "rbs_motif": "GGAGG", "genes": [], "truncated": "3-prime", "pseudogene": { "start": 667334, "stop": 667591, "observations": { "mutation": [ 667490 ], "stop": [ 667490 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001E405388", "query_cov": 1.0, "subject_cov": 0.6046511627906976, "identity": 1.0, "score": 95.9, "evalue": 7.5e-25, "gene_start": 1, "gene_end": 52, "reference_start": 1, "reference_end": 52, "reference_sequence": "MNKKILMTLSLSTLSIIPSCFSLISCTKENKQDEKKSLNQIIEGNENANEREWKNFLNRDYVKEILRLSFDADVDAKNKYIAEQKN" }, "paralog": false, "identity": 0.9882352941176471, "score": 165.0, "evalue": 9.59e-55, "description": "Internal stop codon at 667490. Nonsense mutation around 667490.", "psc": { "uniref90_id": "UniRef90_UPI001E405388", "product": "aromatic motif membrane protein", "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001E405388", "UniRef:UniRef50_UPI001E405388" ] }, "pscc": { "uniref50_id": "UniRef50_UPI001E405388", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001E405388" ], "product": "aromatic motif membrane protein" } }, "id": "GLJLFKCOHC_1142", "locus": "GCA013368035_01143" }, { "type": "cds", "sequence": "contig_1", "start": 667808, "stop": 668014, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAATTTGAATCATCAGATGAACAATCAGCGTTAACCGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAAGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGAGAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTCTTCAATTAACTATTGATTGA", "aa": "MNKFESSDEQSALTAQGNSLILGHFYKPKSNEIIQYTTQVTKNDKNGERTYDVYLLTKEGFILQLTID", "aa_hexdigest": "a92758be7146349171454e00ea7f9761", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7789.6061, "isoelectric_point": 5.216478157043456 }, "id": "GLJLFKCOHC_1143", "locus": "GCA013368035_01144" }, { "type": "cds", "sequence": "contig_1", "start": 668615, "stop": 668737, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGA", "aa": "MSEQKQQAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQ", "aa_hexdigest": "204e7738df961b78ab38eaacecd8719e", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4382.8659, "isoelectric_point": 6.529799842834472 }, "id": "GLJLFKCOHC_1144", "locus": "GCA013368035_01145" }, { "type": "cds", "sequence": "contig_1", "start": 668774, "stop": 668875, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGA", "aa": "MGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFI", "aa_hexdigest": "82e2324bb86e16d48affdc20ce88ce1b", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3821.5944, "isoelectric_point": 9.602904319763184 }, "id": "GLJLFKCOHC_1145", "locus": "GCA013368035_01146" }, { "type": "cds", "sequence": "contig_1", "start": 669074, "stop": 669514, "strand": "+", "frame": 2, "gene": "rpsB", "product": "30S ribosomal protein S2", "db_xrefs": [ "COG:COG0052", "COG:J", "GO:0003735", "GO:0006412", "GO:0015935", "KEGG:K02967", "SO:0001217", "UniRef:UniRef50_D5E5E7", "UniRef:UniRef90_D1J8T8" ], "nt": "ATGGACAAGAAAATTGCCAAGTTGCAAAAGAACTTAGGCGGTATTAGAAAATTAGCTGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCGGCAAACGACGATTCTGTAAAGTCAATTACTTTAATTACAACTATTTTGGCAGACGCAATAGCATCAGCACATGGCGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACTGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATTAGAAAATACTAAGAAATCTGAATAA", "aa": "MDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVELENTKKSE", "aa_hexdigest": "e0964f8621593198634804862b56725f", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "genes": [ "rpsB" ], "truncated": "5-prime", "pseudogene": { "start": 668774, "stop": 669514, "observations": { "mutation": [ 668873, 668954 ], "stop": [ 668873, 668954 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8T8", "query_cov": 1.0, "subject_cov": 0.48344370860927155, "identity": 0.986, "score": 243.0, "evalue": 1.12e-78, "gene_start": 1, "gene_end": 146, "reference_start": 157, "reference_end": 302, "reference_sequence": "MSEQKQQAQPAQPAVAPTQAEPAIVSREKLLEAGTYFGHRVSQWNPKMKQYIWGKRMGIHIIDIAKTQKTLEYAYKLLNKMAQKPISFIWVGTKKQAKKAIESAAARTNSVYVSERWLGGTLTNSQTIFRSVRELERLEELQKSEYQGYTKKEGLLMDKKIAKLQKNLGGIRKLAGKAAMPQVMICASPIDDEIAIREAKKKGLKIFAIQDTNSNPDLVDFVIPANDDSVKSITLITTILADAIASAHGEKPVYAYKSNEEIILPEEQKPEKVEKIANTEERKPRTFVKKANVESENTKKSE" }, "paralog": false, "identity": 0.983739837398374, "score": 431.0, "evalue": 7.56e-155, "description": "Internal stop codon at 668873, 668954. Nonsense mutation around 668873, 668954.", "psc": { "uniref90_id": "UniRef90_D1J8T8", "gene": "rpsB", "product": "30S ribosomal protein S2", "uniref50_id": "UniRef50_D5E5E7", "cog_id": "COG0052", "cog_category": "J", "kegg_orthology_id": "K02967", "go_ids": [ "GO:0003735", "GO:0006412", "GO:0015935" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8T8", "UniRef:UniRef50_D5E5E7", "COG:COG0052", "COG:J", "KEGG:K02967", "GO:0003735", "GO:0006412", "GO:0015935" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5E7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5E7" ], "product": "Small ribosomal subunit protein uS2" } }, "id": "GLJLFKCOHC_1146", "locus": "GCA013368035_01147" }, { "type": "cds", "sequence": "contig_1", "start": 669535, "stop": 669651, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAGTAGATTTAAAAAAAATTAAGGAATTAAGAGAAAGAACAAATTCGGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAACAACGATATTGAAAAAGCAATTGAATGA", "aa": "MSVDLKKIKELRERTNSGFLDVKNALEATNNDIEKAIE", "aa_hexdigest": "70bdda38816f371176ed85c51c179563", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4333.8732, "isoelectric_point": 5.289686775207519 }, "id": "GLJLFKCOHC_1147", "locus": "GCA013368035_01148" }, { "type": "cds", "sequence": "contig_1", "start": 669652, "stop": 670416, "strand": "+", "frame": 1, "gene": "tsf", "product": "translation elongation factor Ts", "db_xrefs": [ "COG:COG0264", "COG:J", "GO:0003746", "GO:0006414", "SO:0001217", "UniRef:UniRef50_Q98Q37", "UniRef:UniRef90_D1J8T9" ], "nt": "TTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCTGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTGATTTTTGAATTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGATGATTTATTAGAACAAAAAATTAATGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGCGAAAAGATTCATTTAAGAAGAGCATTAAAGTATGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAATAGAATTGCTACTATTTTAATTGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCTTTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAAGAAATTTACGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAACAGAAAATAAATTGCAATTAATAGATGCAACAAGATTTGAAGTTGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAA", "aa": "MQEKGIVKAAKKAGRIAAEGIVRAVIKDNIAVIFELNSETDFVAKNQLFLDFAKLLEEELPKVNFSNIDDLLEQKINGKTIEEYTTDLTAKIGEKIHLRRALKYEAKEDEVVAGYTHANNRIATILIAKGHNQEALRHVAMHIAALNPAHLFETCLDEEELKEIYERINNDPKLASKPEKIQVSMKSGMLRKEFNERGVLMYQPFVMEESKTVQQYLTENKLQLIDATRFEVGEGIEKKTVDFATEVAEQMSLK", "aa_hexdigest": "3438d9ef9e9f6effb134627f2937a747", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D1J8T9", "query_cov": 1.0, "subject_cov": 0.8668941979522184, "identity": 0.992, "score": 476.0, "evalue": 8.46e-169, "valid": true, "gene": "tsf", "product": "translation elongation factor Ts", "uniref50_id": "UniRef50_Q98Q37", "cog_id": "COG0264", "cog_category": "J", "go_ids": [ "GO:0003746", "GO:0006414" ] }, "pscc": { "uniref50_id": "UniRef50_Q98Q37", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98Q37" ], "product": "Elongation factor Ts" }, "genes": [ "tsf" ], "id": "GLJLFKCOHC_1148", "locus": "GCA013368035_01149" }, { "type": "cds", "sequence": "contig_1", "start": 670448, "stop": 671275, "strand": "-", "frame": 1, "gene": "whiA", "product": "DNA-binding protein WhiA", "db_xrefs": [ "COG:COG1481", "COG:K", "GO:0003677", "GO:0043937", "RefSeq:WP_044730225.1", "SO:0001217", "UniParc:UPI0005CC9D11", "UniRef:UniRef100_UPI0005CC9D11", "UniRef:UniRef50_D1J8U0", "UniRef:UniRef90_D1J8U0" ], "nt": "ATGGAGAATAAATCTTTTAGTCAACTAGTAAAAGAAGAAATTATGTCTTTTCAGTTAAAAAAACAAGAAAAGTTTTTTTTGATGGCTGGCATAATTTATTGTTGCAATGTAAAAAACAATGAAGCTAAATTAGTAATAAATAATATTAAGATTAGGACATATATATTAAATATTTGTAATGAACTTAAAATTTCTTATACATATACTAAAAAAAATGAAATTTCAATAAATACAAATTTCTTAGCTAACATAAGTGTTAAATATCAAGGAAAATTCTTTGCTGGTTTATTCCTTTCTTCGGGTTCAATAATAAATGGAAGAACAAATCATTTAGAGTTAAAATTTAATGATTTAATAAATGCAGAAAATGCTTTATCAATTTTAAATTCTCACGGATTAGAATTTAAAAAAATAATTAGAAAACAAAAAATAATAATTTATTTAAAAAAAATTGAAAATATTTGTGATTTTTTAAAAGCTATTGAAACCATTGAATCTTATTATTTATTCGAAGATGGCAAAATTCAAAGAGATTTTTATAATTCGGCAAACAGAATAACAAATCTCGACGTATATAATCAAGAACGTATTGCAAAAGCAAATTTAGAATTTATTAAAAATTATCAATTTATAAAAGAAAACAATCTCTTGGATGAGTTTAGCGTTGAAGAACTTACACTATTTGAATTAAAATTAAATAGTTTAGACAGTTCCTTGAATGAACTTAGTTTATTATTAGAAACAAAAAACATTAAAAAGTCTCGTTCTGCATTGAATCATTTCTTAATTAAGCTAAAAAAAATAACTAAAAAGTATCAAAAAAAATAG", "aa": "MENKSFSQLVKEEIMSFQLKKQEKFFLMAGIIYCCNVKNNEAKLVINNIKIRTYILNICNELKISYTYTKKNEISINTNFLANISVKYQGKFFAGLFLSSGSIINGRTNHLELKFNDLINAENALSILNSHGLEFKKIIRKQKIIIYLKKIENICDFLKAIETIESYYLFEDGKIQRDFYNSANRITNLDVYNQERIAKANLEFIKNYQFIKENNLLDEFSVEELTLFELKLNSLDSSLNELSLLLETKNIKKSRSALNHFLIKLKKITKKYQKK", "aa_hexdigest": "bf99c3d455573e7739357d0ed10cfe2c", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI0005CC9D11", "ncbi_nrp_id": "WP_044730225.1", "uniref100_id": "UniRef100_UPI0005CC9D11", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CC9D11", "RefSeq:WP_044730225.1", "UniRef:UniRef100_UPI0005CC9D11" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CC9D11", "uniref90_id": "UniRef90_D1J8U0" }, "psc": { "uniref90_id": "UniRef90_D1J8U0", "gene": "whiA", "product": "DNA-binding protein WhiA", "uniref50_id": "UniRef50_D1J8U0", "cog_id": "COG1481", "cog_category": "K", "go_ids": [ "GO:0003677", "GO:0043937" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U0", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U0" ], "product": "DNA-binding protein WhiA" }, "genes": [ "whiA" ], "id": "GLJLFKCOHC_1149", "locus": "GCA013368035_01150" }, { "type": "cds", "sequence": "contig_1", "start": 671324, "stop": 671851, "strand": "-", "frame": 1, "gene": "pncA", "product": "Isochorismatase-like domain-containing protein", "db_xrefs": [ "COG:COG1335", "COG:HR", "RefSeq:WP_044730224.1", "SO:0001217", "UniParc:UPI0005CD53E5", "UniRef:UniRef100_UPI0005CD53E5", "UniRef:UniRef50_A0A077L748", "UniRef:UniRef90_D1J8U1" ], "nt": "ATGAAAGCAATCTTTGTTGTTGATATGGTTAATGGATTTTGTAAAAAAGGTGCACTATATAGTTGCAATATTGAGTCAATAATTGTTCCTATAAAAAATTTTTTAGAAACTCAATATAAAGACAATGATATATTTTTTTTAAACGATGCTCATAGTAGCGATGATATTGAAATGCAATCATACCCAATACATTGTTTAAAAAATAGCAAAGAAAGTCAAGTAGTAGATGAATTAAAAAAATATGCTAAAAATATAATTGAAAAGAATTCTACTAACTCCTTTTTTGCATTAAAAAAGGAAATTTTAAGCAAGTATGATTCCTTTGAAATTATTGGTTGTTGTTCAGATATTTGCATTTTGCAATTTGCAATAACTCTAAAAACTTATTTCAATCATTTAAAGCAAAATAAAGAAATTGTTGTCTTTAAAAATTTAATTGCAACATTTAATATTCCAAATCATAATTCTCAAGAATATCACGATTTTGCTTTAAATTTAATGGCTAATGCTGGTATAAAAATTAAATAA", "aa": "MKAIFVVDMVNGFCKKGALYSCNIESIIVPIKNFLETQYKDNDIFFLNDAHSSDDIEMQSYPIHCLKNSKESQVVDELKKYAKNIIEKNSTNSFFALKKEILSKYDSFEIIGCCSDICILQFAITLKTYFNHLKQNKEIVVFKNLIATFNIPNHNSQEYHDFALNLMANAGIKIK", "aa_hexdigest": "cb86993d15af95f9790e64ee2df3a832", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI0005CD53E5", "ncbi_nrp_id": "WP_044730224.1", "uniref100_id": "UniRef100_UPI0005CD53E5", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005CD53E5", "RefSeq:WP_044730224.1", "UniRef:UniRef100_UPI0005CD53E5" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005CD53E5", "uniref90_id": "UniRef90_D1J8U1" }, "psc": { "uniref90_id": "UniRef90_D1J8U1", "gene": "PncA", "product": "Isochorismatase-like domain-containing protein", "uniref50_id": "UniRef50_A0A077L748", "cog_id": "COG1335", "cog_category": "HR" }, "pscc": { "uniref50_id": "UniRef50_A0A077L748", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A077L748" ], "product": "Isochorismatase-like domain-containing protein" }, "genes": [ "pncA" ], "id": "GLJLFKCOHC_1150", "locus": "GCA013368035_01151" }, { "type": "cds", "sequence": "contig_1", "start": 672022, "stop": 672174, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGA", "aa": "MAEEQLVVTSSPKKKIGFFSAILIVMGSAIGVGVFLRSKNVLQNSANNVV", "aa_hexdigest": "02596721837798f5e42380334bb00f3d", "start_type": "ATG", "rbs_motif": "AGGA", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5293.2089000000005, "isoelectric_point": 9.996161460876465 }, "id": "GLJLFKCOHC_1151", "locus": "GCA013368035_01152" }, { "type": "cds", "sequence": "contig_1", "start": 672328, "stop": 672459, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCTATCTATATCTACCATTTACTTACTTCTTTATGCCTTACTACGTTATCGTTCAATTCCAAGACGGTATCGCAGGATTCATAGGATACAGCAAAGTAGGATTTGGTGGTTCTACAAGTGCTCCATGA", "aa": "MTYLYLPFTYFFMPYYVIVQFQDGIAGFIGYSKVGFGGSTSAP", "aa_hexdigest": "d37629d4c277e2ad5df48abfe6bee962", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4845.5412000000015, "isoelectric_point": 5.586102104187011 }, "id": "GLJLFKCOHC_1152", "locus": "GCA013368035_01153" }, { "type": "cds", "sequence": "contig_1", "start": 672820, "stop": 672975, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAACACCCAGAAAAAACCCCTGCTGCTATTGTTATTGGGTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAACAACGGTTCATTCTACGGATTCGCAGATGAATTAAACGCTGTTAAAGCAGGTTGA", "aa": "MKHPEKTPAAIVIGLASMTVLYMLIAVTMTLGGNNGSFYGFADELNAVKAG", "aa_hexdigest": "4528d2c402537aae70871edef7bee25b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5315.211200000002, "isoelectric_point": 6.514055442810059 }, "id": "GLJLFKCOHC_1153", "locus": "GCA013368035_01154" }, { "type": "cds", "sequence": "contig_1", "start": 673177, "stop": 673290, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGTTATCTTTACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGA", "aa": "MVIFTAIGAYAYFGGGYSTDYGYQIDNLLTFADLMAN", "aa_hexdigest": "80ebdad5c03bf7b06982cc073cecfbe3", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4048.5029000000013, "isoelectric_point": 4.0500284194946286 }, "id": "GLJLFKCOHC_1154", "locus": "GCA013368035_01155" }, { "type": "cds", "sequence": "contig_1", "start": 673432, "stop": 673677, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCACAATACGTTGCAAATAAAACAGAAGACACTAGAAACGGCTTAGTAGGAAACATTGCTACATCAGTTCTATTTATAATATATGCAGTAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAACATTTGTGCACACGAAGTTCCAGAATGA", "aa": "MFFLAIDPFISIGLSAAQYVANKTEDTRNGLVGNIATSVLFIIYAVVSFLGAPVERQIAKSNKIKFDRVLSGNICAHEVPE", "aa_hexdigest": "fe6400b8bc2df6f2bc71c05b1f30f87a", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8801.109299999998, "isoelectric_point": 6.551114463806152 }, "id": "GLJLFKCOHC_1155", "locus": "GCA013368035_01156" }, { "type": "cds", "sequence": "contig_1", "start": 673878, "stop": 674144, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00690.31" ], "nt": "ATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAGATGAAACATTGATTAACACAAATTTAAAAACTGGCCTAAGTTCACAAGAGGCTGATTTAAGACTTAAAAAGTTTGGAAAAAATGAATTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAGCAATTTAAAGATATATTGGTCATTATATTATTATTTGCAGCAATAATATCTTATGCTCTTGCAATTGTTTCAGGAACTCAAAATAATTGA", "aa": "MKNSKEQINNIPYVLDETLINTNLKTGLSSQEADLRLKKFGKNELHKAKKINPFLIYLEQFKDILVIILLFAAIISYALAIVSGTQNN", "aa_hexdigest": "11c9140809ead45203ef22b93c809fb8", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00690.31", "name": "Cation_ATPase_N", "length": 56, "aa_cov": 0.6363636363636364, "hmm_cov": 1.0, "evalue": 2.6668607960221772e-21, "score": 70.48544311523438, "start": 21, "stop": 76 } ], "seq_stats": { "molecular_weight": 9977.57569999999, "isoelectric_point": 9.26070613861084 }, "id": "GLJLFKCOHC_1156", "locus": "GCA013368035_01157" }, { "type": "cds", "sequence": "contig_1", "start": 674274, "stop": 674783, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGAGCAAATTAAGTCCCTTACAAGCTAAAGTCGTCCGTGATGGCAATTTAATAACATTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGCTATTTGTTGTTTAGTTCAAATTTAACTGTTATCGAGAGTGCATTGACCGGAGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAAAGAAATTTAGCATTAGCAATGGCAGATAGACAATTTATGGTTTATTCTTCAACTGTAGTTGCTACAGGAACTGCTTATTTTGTTGTTACATCAGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAATTAGTTAATCAACAAAAAGAATCAATGACTCCTCTACAAATTAAAATCAATAAATTAGGAAAAATATTTGGATATTCAGGCCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCGGTATCAAGAAATTCCTTTGCTGATACAATGTTTTGA", "aa": "MSKLSPLQAKVVRDGNLITLASSDITVGDIVLIESGDVVPSDGYLLFSSNLTVIESALTGESEPATKDYQAKRNLALAMADRQFMVYSSTVVATGTAYFVVTSVGENNEIGKISKLVNQQKESMTPLQIKINKLGKIFGYSGLALFLASLVTQIVFHAVSRNSFADTMF", "aa_hexdigest": "967030df51c550dd9b8fd29e713174b5", "start_type": "TTG", "rbs_motif": "AGxAG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18190.707799999993, "isoelectric_point": 5.791972923278809 }, "id": "GLJLFKCOHC_1157", "locus": "GCA013368035_01158" }, { "type": "cds", "sequence": "contig_1", "start": 674729, "stop": 674869, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCTAAAATAATTGAAGTAAAAGCAATTAACCCCTCAGGAATTGCAGCAACGGCTAGTGAAATGGCTCCAACTATTGCATTGCCTCAAAACATTGTATCAGCAAAGGAATTTCTTGATACCGCATGGAAAACTATTTGA", "aa": "MAKIIEVKAINPSGIAATASEMAPTIALPQNIVSAKEFLDTAWKTI", "aa_hexdigest": "0a18c7c5b57c8c2d34719a402ebc2fd7", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4855.669899999999, "isoelectric_point": 6.013644981384277 }, "id": "GLJLFKCOHC_1158", "locus": "GCA013368035_01159" }, { "type": "cds", "sequence": "contig_1", "start": 674883, "stop": 675293, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCAAGAAAGAATGCAATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGTAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAATAAGATGACAATTGTAGATGCTTTTGATGGAAGCCAACTACTATCAAATAATAGTGAAATTAAAAACTTCTTAAATATTATAGAATTAGGTTCATTGTGTTGCGATGCAAATTTAACTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTTTCTAATTTTAAATACAAAAACAACCTAGAAATAGAATATAAGCGTTTGCATTTGTTCCCATTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACGACAACAATTATTGA", "aa": "MARKNAIVKNLMAVESLGNCAVICSDKTGTLTQNKMTIVDAFDGSQLLSNNSEIKNFLNIIELGSLCCDANLTRNEKDEYVVVGDPTEIAFLYELEKFSNFKYKNNLEIEYKRLHLFPFDSDRKMKSVINQNDNNY", "aa_hexdigest": "2d7b5a1415aa3084a2c47abec1a2062d", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 15515.485699999983, "isoelectric_point": 5.193060493469236 }, "id": "GLJLFKCOHC_1159", "locus": "GCA013368035_01160" }, { "type": "cds", "sequence": "contig_1", "start": 675303, "stop": 675794, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00702.32" ], "nt": "ATGGGTGCTCCTGAAATAATAATTGAAAAATGTAATTTTGATTCGCCTTCATTAAAAGAAAAATATTTACAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTCTTAGCAATAGCAAGAAAATCCTTAAAAAAGGAAACAATTGAGAAATTTAATGAATTGTCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATTGATCCTCCAAGAGAAAGTGCAAAAGATGCTATTACATTATGCAAACAAGCAGGAATAAAACCTGTTATGATTACTGGTGATAATTTAAATACAGCAAAAGCAATAGCAACAAATCTTGGTATATATAGTGATCAAGATCGTGCTATAACAGGAACTGAACTTTCAAACATTTCTGATGAAGAATTAGTTCAAAATATTGAAAAATACAGTGTTTATGCTAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCTTGA", "aa": "MGAPEIIIEKCNFDSPSLKEKYLQINNQWANNAYRVLAIARKSLKKETIEKFNELSISKLQEHVENDLEIVGLVAMIDPPRESAKDAITLCKQAGIKPVMITGDNLNTAKAIATNLGIYSDQDRAITGTELSNISDEELVQNIEKYSVYARVKPEDKLRIVNA", "aa_hexdigest": "ddee433b747e7eb7312d5b18e1cdb0bd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00702.32", "name": "Hydrolase", "length": 136, "aa_cov": 0.8159509202453987, "hmm_cov": 0.8676470588235294, "evalue": 5.1987726452964996e-12, "score": 41.943267822265625, "start": 29, "stop": 161 } ], "seq_stats": { "molecular_weight": 18218.646199999996, "isoelectric_point": 5.331520271301269 }, "id": "GLJLFKCOHC_1160", "locus": "GCA013368035_01161" }, { "type": "cds", "sequence": "contig_1", "start": 675819, "stop": 676097, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGACCGGTGATGGCGTTAATGATGCTCCAGCATTAAAAGCTTCAGATATAGGCTGCGCTATGGGTATAACAGGAACTGAAGCTTCTAAGCAGGCTGCAAATGTTATATTGGCAGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAACGGAAGATCAATTTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCAATTGCAGAGATCATTTTATTATTTATAGGTTTATTTTTGTTTAAAGCGATTTTTTCAAGTGCTGAATGA", "aa": "MTGDGVNDAPALKASDIGCAMGITGTEASKQAANVILADDNFATIVSAVANGRSIYQKIKNVIQSLLITSIAEIILLFIGLFLFKAIFSSAE", "aa_hexdigest": "d5e62797fa58ea977c0fde073636f9ca", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9562.986599999993, "isoelectric_point": 4.842193412780761 }, "id": "GLJLFKCOHC_1161", "locus": "GCA013368035_01162" }, { "type": "cds", "sequence": "contig_1", "start": 676533, "stop": 676778, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGCTACACCGAAATTCATTCTAAGCCCGAACTTTTAGCACTAGGATTAGCATTACCATTAGTAATTATTCCAATATATTCAATTCATAAATTAATCATATATTTAATCGAAAAAAACAAAATTAAAGAAAATAAAATTACAGAATTTAAAATGATATTACCTCCAAAGCAAGTCAAAAGAAATCAAAAAAATGTAGATCTATAG", "aa": "MIVVFVKNINLVFGCYTEIHSKPELLALGLALPLVIIPIYSIHKLIIYLIEKNKIKENKITEFKMILPPKQVKRNQKNVDL", "aa_hexdigest": "f70518fd76fb1310bed3534aef7ec7a9", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9384.37839999999, "isoelectric_point": 9.630303382873535 }, "id": "GLJLFKCOHC_1162", "locus": "GCA013368035_01163" }, { "type": "cds", "sequence": "contig_1", "start": 676841, "stop": 677206, "strand": "-", "frame": 1, "gene": null, "product": "Zinc-ribbon domain-containing protein", "db_xrefs": [ "RefSeq:WP_044420832.1", "SO:0001217", "UniParc:UPI0005C9394A", "UniRef:UniRef100_UPI0005C9394A", "UniRef:UniRef50_D1J8U5", "UniRef:UniRef90_D1J8U5" ], "nt": "ATGAATTATTGCCCAAATTGTGGCACTAAATTAAATAATGCTAATTATTGCCCAAATTGCGGAAAAAAGGTGGAAAAAGATGCATATGTAACAACACAAATGAATACAACGCAACCGTCTCAGCCTTATTTGCAACCTCAACAACAAATCCAAAGTTCAGAAACTAATGGATTTGCTATTGCCGGATTTATATTGTCGTTTTTCTTTGCGATGTTGGGTCTTATTTTTAGCTGTATTGGACTTAGCAAATCAAAATCAATGAATGATTCAGGAAGAACTTTGTCAATAGCAGGAATTATTATTTCTTGCTTAACTATGTTTTTTGCCTTTGTTGTATTTGTTACTGTAATGAGTGTAAGCCATTAG", "aa": "MNYCPNCGTKLNNANYCPNCGKKVEKDAYVTTQMNTTQPSQPYLQPQQQIQSSETNGFAIAGFILSFFFAMLGLIFSCIGLSKSKSMNDSGRTLSIAGIIISCLTMFFAFVVFVTVMSVSH", "aa_hexdigest": "855dfc02ae5d6ebfb256ae3ac8f1f8ab", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "ups": { "uniparc_id": "UPI0005C9394A", "ncbi_nrp_id": "WP_044420832.1", "uniref100_id": "UniRef100_UPI0005C9394A", "db_xrefs": [ "SO:0001217", "UniParc:UPI0005C9394A", "RefSeq:WP_044420832.1", "UniRef:UniRef100_UPI0005C9394A" ] }, "ips": { "uniref100_id": "UniRef100_UPI0005C9394A", "uniref90_id": "UniRef90_D1J8U5" }, "psc": { "uniref90_id": "UniRef90_D1J8U5", "product": "Zinc-ribbon domain-containing protein", "uniref50_id": "UniRef50_D1J8U5" }, "pscc": { "uniref50_id": "UniRef50_D1J8U5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U5" ], "product": "Zinc-ribbon domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_1163", "locus": "GCA013368035_01164" }, { "type": "cds", "sequence": "contig_1", "start": 677322, "stop": 677414, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGAATGTAAGGAAATGCACTTTATGCAACTTGACGGCGAAACATTTGATATAGAAAATAAAATATATATAAACAAAAAAAGAATCAATTAA", "aa": "MECKEMHFMQLDGETFDIENKIYINKKRIN", "aa_hexdigest": "792aa9df189a1e6b36362e2805f492ff", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 3719.2922000000017, "isoelectric_point": 5.596105766296386 }, "id": "GLJLFKCOHC_1164", "locus": "GCA013368035_01165" }, { "type": "cds", "sequence": "contig_1", "start": 677616, "stop": 678206, "strand": "-", "frame": 3, "gene": "lCB5", "product": "Transcriptional regulator", "db_xrefs": [ "COG:COG1597", "COG:IR", "SO:0001217", "UniRef:UniRef50_D1J8U6", "UniRef:UniRef90_D1J8U6" ], "nt": "ATGCTATATATATTTTATAATTCTCTATCAAAGAGTGGAAAATGTCAAAGAAAGATGTATGACATAGTTTCTGACGCCATTAAAGTATTTTTACAACCTGAGGTAAAAATATTTGACATTATAAAAATTAAAGACAGTCAAAATCTAGTTTCTCAAATGGATATAGAAAAAGATATTGTTTTATTAATTGGCGGTGATGGAACATTAACCAATATCACTAATAAAATTCACAATATAAAACCTTTACCCAAAATTTATGCATATAAAGCAGGAACGGGCAATGATTTTCTAAGGTCTTTAAAAATAAAACAAGATGTTCAAATAATACATAAAAAATATTATTTGATATCTCCTTACATTGCTAACCTACCAACAATTAAATTTAAAAACGACCAAGATGAAAGATCTTTTTTAAATGGCATTGGTTTTGGCATTGATGCAGTTATTGCAAAAAAAGTTAATGATGAAAAAATGCTTAAAAATAAATCATCATTTGCTAAAGTAACAATAAATGCATTTAAGACATTCAAAACAATTCCTTTAATAAAAATTAATGTAGATGGAAAAGAATTAGAATTTAAAAATGTTTGA", "aa": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYIANLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNV", "aa_hexdigest": "efc3829b4a98d8303decf518426e66b6", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "genes": [ "lCB5" ], "truncated": "5-prime", "pseudogene": { "start": 677314, "stop": 678206, "observations": { "deletion": [ 677336 ], "mutation": [ 677495, 677507, 677618 ], "stop": [ 677495, 677507, 677618 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U6", "query_cov": 1.0, "subject_cov": 0.6068111455108359, "identity": 1.0, "score": 385.0, "evalue": 2.03e-133, "gene_start": 1, "gene_end": 196, "reference_start": 1, "reference_end": 196, "reference_sequence": "MLYIFYNSLSKSGKCQRKMYDIVSDAIKVFLQPEVKIFDIIKIKDSQNLVSQMDIEKDIVLLIGGDGTLTNITNKIHNIKPLPKIYAYKAGTGNDFLRSLKIKQDVQIIHKKYYLISPYIANLPTIKFKNDQDERSFLNGIGFGIDAVIAKKVNDEKMLKNKSSFAKVTINAFKTFKTIPLIKINVDGKELEFKNVWLASIMNNCYFGGGMKLAPNCNNTDKLNLIIINNVSKWKLAWLFFSVYSGKHLRYKKYVHEIQGYKFSMECKEMHFMQLDGETFDIENKIYINKKKNQLNKKRPNKNWDVFLLLYCVIFIYKINLKK" }, "paralog": false, "identity": 0.98989898989899, "score": 578.0, "evalue": 4.09e-212, "description": "Internal stop codon at 677495, 677507, 677618. Frameshift due to deletion around 677336. Nonsense mutation around 677495, 677507, 677618.", "psc": { "uniref90_id": "UniRef90_D1J8U6", "gene": "LCB5", "product": "Transcriptional regulator", "uniref50_id": "UniRef50_D1J8U6", "cog_id": "COG1597", "cog_category": "IR", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U6", "UniRef:UniRef50_D1J8U6", "COG:COG1597", "COG:IR" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8U6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8U6" ], "product": "Transcriptional regulator" } }, "id": "GLJLFKCOHC_1165", "locus": "GCA013368035_01166" }, { "type": "cds", "sequence": "contig_1", "start": 678210, "stop": 678956, "strand": "-", "frame": 3, "gene": "argE", "product": "Xaa-His dipeptidase", "db_xrefs": [ "COG:COG0624", "COG:E", "SO:0001217", "UniRef:UniRef50_A0A448ZY24", "UniRef:UniRef90_D1J8U7" ], "nt": "ATGATCTATGTAACAGAAATTCATTTTCCAAAGGTTCCAGGAGTTGAATTAAATGGGGGCGACGTTGTTAACCAAATTCCTGATTCAACATCTTTAGTATTGAATGAAAAACCTAATATTATTAAGGGAATTGGTGGACACGGCTCAACGCCTGAAAAAGGTGACAATGCGATAATTAAAGCCATTAATATAGCAAGCAAAGAAAATAAAGAATTTGCTAAATCTAAATTATTTAAATTTATTAAAGAAAATTTAAATAGCAAATCATTTAATTTAAGTAATATTTTTAAAAACTATAGTGATTTTTCGGGCGAATTATCTGCAAATTTAGGCATTATTAGAACCTTGAAAAATGAATATGTTTTAACTTTTGATTTTAGAGTCCCAGTTTCTCATAAAATTGATGATGTAACCAAAAGCTTAAATGATTATTTAAAAGATGAATTTGGCGATGATGTATTTTCAAAATTGCAAGGATTCAAAGAACCAAAGTATATTGAACAAGATTCTCCAATTGTAAGAATTCTTATGGATACATATAATGAAGCGATGAATTCAAATGAAAAACCTTTAGCAATTGGTGGCGGTACATTTGCAAGATTAATAAAAACTTGTGTCGCTTTTGGATCAACTAAATATATGCATTTAATGCATGGACCAAACGAATATTTTACATTTGATGAAATAAAATCATCACTTGAAATTTATATCAATGCATTAAATAGATTACAAGATTTGGAAAAATAA", "aa": "MIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGDDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEK", "aa_hexdigest": "5a54aa0d90e982c4bb7cec90702eb2d2", "start_type": "ATG", "rbs_motif": null, "genes": [ "argE" ], "truncated": "3-prime", "pseudogene": { "start": 678210, "stop": 679256, "observations": { "mutation": [ 678980, 679091, 679229 ], "stop": [ 678980, 679091, 679229 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8U7", "query_cov": 1.0, "subject_cov": 0.5662100456621004, "identity": 0.992, "score": 485.0, "evalue": 3.62e-170, "gene_start": 1, "gene_end": 248, "reference_start": 191, "reference_end": 438, "reference_sequence": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQWRTSAFVPVITNESIIGRGSLDDKGPAIINLYAMKYIHDHGLLSNEWKIRIIFGISEETTMKSMKSYLADFKDPYISYTPDGEWPLIYAEKMIYVTEIHFPKVPGVELNGGDVVNQIPDSTSLVLNEKPNIIKGIGGHGSTPEKGDNAIIKAINIASKENKEFAKSKLFKFIKENLNSKSFNLSNIFKNYSDFSGELSANLGIIRTLKNEYVLTFDFRVPVSHKIDDVTKSLNDYLKDEFGNDVFSKLQGFKEPKYIEQDSPIVRILMDTYNEAMNSNEKPLAIGGGTFARLIKTCVAFGSTKYMHLMHGPNEYFTFDEIKSSLEIYINALNRLQDLEE" }, "paralog": false, "identity": 0.985632183908046, "score": 681.0, "evalue": 6.97e-250, "description": "Internal stop codon at 678980, 679091, 679229. Nonsense mutation around 678980, 679091, 679229.", "psc": { "uniref90_id": "UniRef90_D1J8U7", "gene": "ArgE", "product": "Xaa-His dipeptidase", "uniref50_id": "UniRef50_A0A448ZY24", "cog_id": "COG0624", "cog_category": "E", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8U7", "UniRef:UniRef50_A0A448ZY24", "COG:COG0624", "COG:E" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZY24", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZY24" ], "product": "Dipeptidase SA1572" } }, "id": "GLJLFKCOHC_1166", "locus": "GCA013368035_01167" }, { "type": "cds", "sequence": "contig_1", "start": 679227, "stop": 679526, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTTATTACTTATAAGCAAAGTAAAGAAGACTTTGATGAAATGATTAAAAATATTGCAAATATTTGTGCAATTCCTTCGATTAGTGAAGAAGATTTTAGTAGTGAATTTCCTTTTGGAAAAGAAACTGACAATGCTTTAAATTATGCATTAAATCTTGCCAAAAGTTTTGGATTTAATATTTATAAAGATCCTAAAAATCGTTATGGGTTTGCTGAATTTGGCAATGGAGATAAGATTATTGGAATACTAGCCCACCTTGACGTTGTTCCTGCAGGAGATGAAAGCCAATGA", "aa": "MKKFITYKQSKEDFDEMIKNIANICAIPSISEEDFSSEFPFGKETDNALNYALNLAKSFGFNIYKDPKNRYGFAEFGNGDKIIGILAHLDVVPAGDESQ", "aa_hexdigest": "6d5702e55358a66b66de891d7d2c573f", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 11122.363599999988, "isoelectric_point": 4.749943733215332 }, "id": "GLJLFKCOHC_1167", "locus": "GCA013368035_01168" }, { "type": "cds", "sequence": "contig_1", "start": 679777, "stop": 680016, "strand": "-", "frame": 2, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8", "UniRef:UniRef90_UPI00068CCCF8" ], "nt": "TTGCAAGGCAACAAAATATCCAAAAACGGTACAAAAACTGGTTTGTCAGTTGAAGATGGTGGATCTGTTACTGAACCTATTAATGAATTTAAGGGTGATGTTGCTAGGGCATATTTATACTTTTGTTTAACTTATAACGATTTAAATATAACATCAAATAATCAATCAACTCGTGTATTTAAAGAAAATAATGGAAAAAATACTATAACTCCTATATTTTTAAAAACATTTTTAGCTTGA", "aa": "MQGNKISKNGTKTGLSVEDGGSVTEPINEFKGDVARAYLYFCLTYNDLNITSNNQSTRVFKENNGKNTITPIFLKTFLA", "aa_hexdigest": "ed3e31b222f48c697241ea2ce5c22306", "start_type": "TTG", "rbs_motif": "GGA/GAG/AGG", "genes": [], "truncated": "both", "pseudogene": { "start": 679603, "stop": 680084, "observations": { "deletion": [ 680069 ], "stop": [ 679778 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00068CCCF8", "query_cov": 1.0, "subject_cov": 0.49375, "identity": 0.9490000000000001, "score": 149.0, "evalue": 1.42e-44, "gene_start": 1, "gene_end": 79, "reference_start": 24, "reference_end": 102, "reference_sequence": "MFDQPDKLVNAWHANYPYGTVKELQGNKISKNGTKTGLSVEDGGPVTEPINEFKGDVARAYLYFCLTYNDLNITSNNQSTRVFKEINRKNTITPIFLKTFLAWNNLDKVSKFDIDRNNGVYKHQGNRNPFIDYPKLIDVVYNNNNDYVFKNKGIAVGLNK" }, "paralog": false, "identity": 0.96875, "score": 315.0, "evalue": 1.2e-112, "description": "Internal stop codon at 679778. Frameshift due to deletion around 680069.", "psc": { "uniref90_id": "UniRef90_UPI00068CCCF8", "product": "endonuclease", "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00068CCCF8", "UniRef:UniRef50_UPI00068CCCF8" ] }, "pscc": { "uniref50_id": "UniRef50_UPI00068CCCF8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00068CCCF8" ], "product": "endonuclease" } }, "id": "GLJLFKCOHC_1168", "locus": "GCA013368035_01169" }, { "type": "cds", "sequence": "contig_1", "start": 680197, "stop": 680649, "strand": "-", "frame": 2, "gene": null, "product": "endonuclease", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A5CC56", "UniRef:UniRef90_UPI0034A5CC56" ], "nt": "ATGAAAAAATCCAAAAAAATATTCCTAACTATGGGTGCTATTTTACCTTTGCTAATTTCAGCGCCATTAGTTGCTGCTGGATGTGTAAAACCTGAAGTTAAACCAGAAGGTGAAAAACCAAAAACAACACCAGAAAAGAAACCAGAAGTTAAACCAGAAGGTGAAAAACCAAAAACAACACCAGAAAAGAGCCCTGAAGCTAAATCCTTAGATAATAAGATAATTTACGATAATTCAAACGACTTTTATAAATCCTTAGATGGCTTAAGTGGACAAAACCTAAGAGATAACTTATTTGCATTGCAAAAAGCTCATAGAAATCACTCAACAAAATATAATGACTTATTTGATACATATAAAGATGCTTTTGTTGATAAATATTATGAAAAAGATGGATCTGTTTTAGATATCTATGAAGAAATCCCAGATAAAGAAGATGTTTTTACTAATTGA", "aa": "MKKSKKIFLTMGAILPLLISAPLVAAGCVKPEVKPEGEKPKTTPEKKPEVKPEGEKPKTTPEKSPEAKSLDNKIIYDNSNDFYKSLDGLSGQNLRDNLFALQKAHRNHSTKYNDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTN", "aa_hexdigest": "404d2ce95ab51772c8f1f772502422e6", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 680068, "stop": 680649, "observations": { "mutation": [ 680079, 680124, 680199 ], "stop": [ 680079, 680124, 680199 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI0034A5CC56", "query_cov": 1.0666666666666667, "subject_cov": 0.7843137254901961, "identity": 0.9129999999999999, "score": 231.0, "evalue": 2.9e-75, "gene_start": 1, "gene_end": 150, "reference_start": 1, "reference_end": 160, "reference_sequence": "MKKSKKIFLTMGAILPLLISTPLVAAGCVKPEAKPESEKPGTTPEKKPEVKPEGEKPKTTPEKSPEAKPEKPKMPEAKSLDNKIIYDNSNDFYKSLDGLSGQNLRDNLFALQKAHRNHSTKYNDLFDTYKDAFVDKYYEKDGSVLDIYEEIPDKEDVFTNWHGKYRDTGKREGQGMNREHLVPQSWFRKSAPMRTDAHHVWPTG" }, "paralog": false, "identity": 0.8719211822660099, "score": 320.0, "evalue": 7.85e-113, "description": "Internal stop codon at 680079, 680124, 680199. Nonsense mutation around 680079, 680124, 680199.", "psc": { "uniref90_id": "UniRef90_UPI0034A5CC56", "product": "endonuclease", "uniref50_id": "UniRef50_UPI0034A5CC56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI0034A5CC56", "UniRef:UniRef50_UPI0034A5CC56" ] }, "pscc": { "uniref50_id": "UniRef50_UPI0034A5CC56", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0034A5CC56" ], "product": "endonuclease" } }, "id": "GLJLFKCOHC_1169", "locus": "GCA013368035_01170" }, { "type": "cds", "sequence": "contig_1", "start": 680806, "stop": 680919, "strand": "-", "frame": 2, "gene": "marR", "product": "MarR family transcriptional regulator", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035", "UniRef:UniRef50_O85035", "UniRef:UniRef90_O85035" ], "nt": "ATGAGAAAAGACGTTAGATTAGCTAACAAGGTAAAAGCAGCTAAAAGAGAACATAAATTAGCTTTAGAAAAAGCCAGAGCTCGCCGTAGACTTGCTAAACAACAAGCTGAATAA", "aa": "MRKDVRLANKVKAAKREHKLALEKARARRRLAKQQAE", "aa_hexdigest": "96ab6046e0d54d5a6a1bff54ed7fd2fb", "start_type": "ATG", "rbs_motif": "AGGAG", "ups": { "uniparc_id": "UPI00000B85C5", "uniref100_id": "UniRef100_O85035", "db_xrefs": [ "SO:0001217", "UniParc:UPI00000B85C5", "UniRef:UniRef100_O85035" ] }, "ips": { "uniref100_id": "UniRef100_O85035", "uniref90_id": "UniRef90_O85035" }, "psc": { "uniref90_id": "UniRef90_O85035", "product": "MarR family transcriptional regulator", "uniref50_id": "UniRef50_O85035" }, "pscc": { "uniref50_id": "UniRef50_O85035", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_O85035" ], "product": "MarR family transcriptional regulator" }, "genes": [ "marR" ], "id": "GLJLFKCOHC_1170", "locus": "GCA013368035_01171" }, { "type": "cds", "sequence": "contig_1", "start": 680956, "stop": 681195, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGATTAAAAAATCCTATAATGAAGATATTCAACATTTAAATAATGAAGAACAAGAAGAACTATTTCAAATATCTACTGAAGATATTGAATTACCAAATGTTTCAAATAATGTTAATGACAACCAAAAAGATAAAAAAAATATAGCAACAAAAGAAAGCGTTAGTCAGAAAATACAAGAAATTGAAAAAATAGATCTTGAAACAATAATGCAAAAAATTAAACAAATTAAGAAAAAATAG", "aa": "MIKKSYNEDIQHLNNEEQEELFQISTEDIELPNVSNNVNDNQKDKKNIATKESVSQKIQEIEKIDLETIMQKIKQIKKK", "aa_hexdigest": "edd27fcc6bcd566397502f9ba9fa8422", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9343.429799999998, "isoelectric_point": 5.167255592346189 }, "id": "GLJLFKCOHC_1171", "locus": "GCA013368035_01172" }, { "type": "cds", "sequence": "contig_1", "start": 681391, "stop": 681891, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTCAAAATTAGGATATGCCAAAAAAGTTAATTTAAGAGATTTTGAAAATAAATCTTCTCTTAAAACAAAAACTTGCATGTCGTTTAAGGATGATAATGATGAATTAATAGATGCCCAAATTTCTAATGATGAAAAAATGCTATTTATTTTACTAAATAATGGTATGTATCATTTAGTTTCAGAAAACGAACTAAAGGTTGGAATTTCTTTGAAAGCAAGAGGCATTAGACTTCTTTTAAACTTATATAAACATCCTCAACTTCAAGTAAGTGGTTTTATAACGGTTTCAAAATACAACAATATAATTTATTTAACGCAAGGTGGCTATATAAAATGTTGGGATACTAGCAAATTAGAATTGACCACACGCAATACTCCAAAAATGTTGTTTACGCCACTAAAAAATAATATTTTAGGTCTTCAATCACTTGCTGTTACATTGAGCAATTTAAAAATGTTATACACTAATAATAATGGTAATTTGGCAGAATATGATTGA", "aa": "MSKLGYAKKVNLRDFENKSSLKTKTCMSFKDDNDELIDAQISNDEKMLFILLNNGMYHLVSENELKVGISLKARGIRLLLNLYKHPQLQVSGFITVSKYNNIIYLTQGGYIKCWDTSKLELTTRNTPKMLFTPLKNNILGLQSLAVTLSNLKMLYTNNNGNLAEYD", "aa_hexdigest": "19ee8a9912a9c2868d29e2cbc7d44e7f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18934.766600000003, "isoelectric_point": 9.24349308013916 }, "id": "GLJLFKCOHC_1172", "locus": "GCA013368035_01173" }, { "type": "cds", "sequence": "contig_1", "start": 682000, "stop": 682311, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCTTGAAATAAAAAATCAATATTCAAAGCCAAGATTAACAGAAATTTCAGATCAAAAAATCGATAAAGAAATTGATCATAAATTATTGACAAAAAATGAAGATTTTTATTTATATATAACCAAAGATGGATATTATAAAAAAATAAGTTTAAAAGTTTACACTAGCAATGAATTAAACACATTCAAATTAAAAGAAGAAGATAATGTTTTCTATTTTGATAAAGTAAACTCGTTATCAAAGATATTATTCTTTACAAATTTAGGGAATTATTTTATTATTGATTGCCATTTGTTTAAAGATTGCAATTGA", "aa": "MLEIKNQYSKPRLTEISDQKIDKEIDHKLLTKNEDFYLYITKDGYYKKISLKVYTSNELNTFKLKEEDNVFYFDKVNSLSKILFFTNLGNYFIIDCHLFKDCN", "aa_hexdigest": "765b64ecf6ef4be0ec11af47519c0fcb", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12428.095899999984, "isoelectric_point": 6.718846321105957 }, "id": "GLJLFKCOHC_1173", "locus": "GCA013368035_01174" }, { "type": "cds", "sequence": "contig_1", "start": 682339, "stop": 683421, "strand": "-", "frame": 2, "gene": null, "product": "DNA gyrase subunit A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "nt": "ATGCATGGTAATAAAGGTTCAATTGATGATGACCCTGCCGCTGCAATGCGTTACACTGAATCAAGACTTGAAAAAATTAGTGAACTGATGTTGAGAGATTTAGACAGAAAAGTTGTAAAAATGGCTCCAAACTTTGATGACTCTGAATACGAACCAATTGTTTTGCCGGCCTTATTTCCTAATTTATTAGTTAACGGTGCTAAAGGAATTGCCGCTGGTTTTGCTACAGAAATCCCACCACATAATCTAGGCGAAGTTATTGATGCAACAATTGCATTAATCAAAAATCCTACAATATCAATTGAAGAATTAAGTGAAATAGTTAAAGGCCCAGATTTCCCAACAGGAGCAATTATTAATGGTATAAATGAAATAAAAAAAGCTCTTTCAAGTGGGCAAGGTAGAATTACAATTTCTTCGAAATATCATTATGTTTATGATAAAAAAGATGAATCGAAAATTATTGGTATTGAAATAATTGAAATTCCTTTTGGGGTTGTTAAATCAAAATTAGTTGCCGACATTGATGCAATTGCAATAGATAAAAAAATTTCTGGTATTAAAGAAGTTTTGGACCAAACAGATAGAAACGGAATTTCAATATTTATTCAATTAGAAGACGGCGCAAATGCTGACGCAATAATTGCATACTTAATGAATAAAACCGAACTAAGCATCTCGTATAGTTATAACATGGTTGCAATTGACAATAACCGTCCAGTAATTTTGAATCTCTATAGTGCCTTAATTGCTTATTTAAGTCATTTAAAAGAAGTTAATATAAATGGTATTAATTATGATTTAAAGAAGTTTAAATTGAGACTAGAAATAGTTGAAGGGTTCATTAAAGTAGCCGAAATTTCTGATGAAGTTATACATTTGATTAAAGAAAGCGATAACTCAAAAAAAGGTGTTATCCTTGCATTGATGAATAAATTTAAATTTAGTGAATTGCAAGCAACAGCGATTGCTGAATTAAGATTGTATAAGCTTTCAAGAATGGATCAAATCGAATTTCAAGAAGAAAAGAAAAACCTTGAAATTCAAATTGAAAATTGCAATAAATTATTAAATGATAAATGA", "aa": "MHGNKGSIDDDPAAAMRYTESRLEKISELMLRDLDRKVVKMAPNFDDSEYEPIVLPALFPNLLVNGAKGIAAGFATEIPPHNLGEVIDATIALIKNPTISIEELSEIVKGPDFPTGAIINGINEIKKALSSGQGRITISSKYHYVYDKKDESKIIGIEIIEIPFGVVKSKLVADIDAIAIDKKISGIKEVLDQTDRNGISIFIQLEDGANADAIIAYLMNKTELSISYSYNMVAIDNNRPVILNLYSALIAYLSHLKEVNINGINYDLKKFKLRLEIVEGFIKVAEISDEVIHLIKESDNSKKGVILALMNKFKFSELQATAIAELRLYKLSRMDQIEFQEEKKNLEIQIENCNKLLNDK", "aa_hexdigest": "205914d130ca573767ea57f0a559b380", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0021B04E97", "query_cov": 0.8611111111111112, "subject_cov": 1.0064935064935066, "identity": 0.506, "score": 299.0, "evalue": 4.35e-97, "valid": false, "product": "DNA gyrase subunit A", "uniref50_id": "UniRef50_UPI0021B04E97" }, "pscc": { "uniref50_id": "UniRef50_UPI0021B04E97", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI0021B04E97" ], "product": "DNA gyrase subunit A" }, "genes": [], "id": "GLJLFKCOHC_1174", "locus": "GCA013368035_01175" }, { "type": "cds", "sequence": "contig_1", "start": 683575, "stop": 683757, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00521.26" ], "nt": "ATGAAAAAAGATAGAAAAGAAGAAATACAAGAAGTTACTGAAAACATTATTGAAAAAAATATGGCCGATATAATGTCTGATAGATTCGGACGTTATTCAAAATACATTATTCAACAAAGAGCAATTCCTGATGCTCGTGATGGACTAAAACCTGTTCAACGTCGGATTTTATATTCAATGTGA", "aa": "MKKDRKEEIQEVTENIIEKNMADIMSDRFGRYSKYIIQQRAIPDARDGLKPVQRRILYSM", "aa_hexdigest": "8d3587316b5efea3fb752e3d36364935", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00521.26", "name": "DNA_topoisoIV", "length": 21, "aa_cov": 0.35, "hmm_cov": 1.0, "evalue": 1.0205122744290668e-10, "score": 36.42316436767578, "start": 40, "stop": 60 } ], "seq_stats": { "molecular_weight": 7217.295900000001, "isoelectric_point": 9.451403617858887 }, "id": "GLJLFKCOHC_1175", "locus": "GCA013368035_01176" }, { "type": "cds", "sequence": "contig_1", "start": 683820, "stop": 683930, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAACCCCGAAACTCGTTCAATAATTAAAGTTGATGTAGAAGATGAAATTTTAGTTGAAAGAAATGTTTCAACATTGATGAGTGACGACGTAATAGCAAGAAAACAATGA", "aa": "MNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQ", "aa_hexdigest": "099d210941b061bc58644c32aaae5669", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4144.682400000001, "isoelectric_point": 4.507070732116699 }, "id": "GLJLFKCOHC_1176", "locus": "GCA013368035_01177" }, { "type": "cds", "sequence": "contig_1", "start": 683940, "stop": 684134, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAACTAGTTGAAAACGGTATGGTTTATATTGCTTTGCCACCTCTTTATAAGATTTCAATAAAATCAAAAAATACACAGCCCCTTTATGCTTGGGATGAAGATCAATTAAAAGAAATTACATCAAAATATACCAACTATGAAATTCAACGCTACAAAGGGCTTGGCGAGATGAATGCTGACCAGCTTTGA", "aa": "MKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQL", "aa_hexdigest": "62171427d03e6284d882c45f788b6bc4", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7514.605900000001, "isoelectric_point": 7.907514762878418 }, "id": "GLJLFKCOHC_1177", "locus": "GCA013368035_01178" }, { "type": "cds", "sequence": "contig_1", "start": 684141, "stop": 685097, "strand": "-", "frame": 3, "gene": "gyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "db_xrefs": [ "COG:COG0187", "COG:L", "SO:0001217", "UniRef:UniRef50_Q59961", "UniRef:UniRef90_D1J8V0" ], "nt": "ATGCCAAATCTTACTATTGAATTTTATAATGAAAATACCGATGAACATGTAACATTTAAAACCAAAGATGGCATAAAAGAATATATAAATTTTGTTGGTGAAGGCAAAAAATTTATTAGTGAAATTTTTTATGCATCTGGTATGTGCAATTACGGCATTGAAATTGACTTGGCATTAGTATATACTGATGGATATAGTGAATCAATTTACAGTTTTGTAAATAATGTAAAGACTCGTGATGGTGGAACACATGAAACAGCACTTAAAACCGCATTAACTAAGACAATAAATGATTATGCTTCTAAAAATGGTTTAGCTAAGAAAATAACTTTTGAAGGCTCGGATGTTAGAGAAGGAATAATTGCTGTTATTTCTTTAAAAGTCCCAGAAGAAATATTAGAATTTGTAGGTCAAACGAAAGATAAATTAGGTACGCCAGAGGCTAGAGAAGTTGTTGAAGATTTTGTAAGTCAAAAGTTTTATTTCTTTTTAAATGAAAACAAAATTGAAGCAGAAAAAATTATTTCTAAAATTAAAAAAGCATACGAAGCAAAAGTTGCAGCTAGAAACGCAAGAAACGAAGCACGTAAGATTAAAAATAAATTTGAGAATAGAAAGATTCTTTCAGGAAAATTAACTCCTGCTCAAAGTAAAAATCCTGCCGTTAAAGAATTGTTTTTAGTTGAAGGTGATTCAGCTGGCGGTTCTGCTAAATTAGGAAGAAATAGAGTTACCCAGGCAATATTGCCACTTAGAGGCAAAGTTATCAACACCGATAAAGCAAAATTAAGCGATGTTTTAGCAAATGAAGAAATTGCAACAATAATTAATACAATCGGAGCGGGTATAGGTGAAGACTTCAATTTAAAAAATGCTCAATATCACAAAATTATTATTATGACAGATGCTGACACTGATGGTGCTCACATTCAAACATTACTATTAACATTCTTTTGA", "aa": "MPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAVKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFF", "aa_hexdigest": "affae0f700569dfb94e210f1993c8744", "start_type": "ATG", "rbs_motif": "3Base/5BMM", "psc": { "uniref90_id": "UniRef90_UPI001E534FD0", "query_cov": 0.9842767295597484, "subject_cov": 0.8368983957219251, "identity": 0.534, "score": 318.0, "evalue": 4.62e-104, "valid": false, "product": "toprim domain-containing protein", "uniref50_id": "UniRef50_A0A380FFC7" }, "genes": [ "gyrB" ], "truncated": "both", "pseudogene": { "start": 683838, "stop": 685397, "observations": { "mutation": [ 683942, 684143, 685178, 685283 ], "stop": [ 683942, 684143, 685178, 685283 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8V0", "query_cov": 1.0, "subject_cov": 0.49765258215962443, "identity": 0.997, "score": 609.0, "evalue": 5.1e-215, "gene_start": 1, "gene_end": 318, "reference_start": 196, "reference_end": 513, "reference_sequence": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTVWRDKKAYFTAFQQDTIIEKTHEISPTTKQGTKVQFWADFSIFKKSKYSPEIIKEKLREASFLMPNLTIEFYNENTDEHVTFKTKDGIKEYINFVGEGKKFISEIFYASGMCNYGIEIDLALVYTDGYSESIYSFVNNVKTRDGGTHETALKTALTKTINDYASKNGLAKKITFEGSDVREGIIAVISLKVPEEILEFVGQTKDKLGTPEAREVVEDFVSQKFYFFLNENKIEAEKIISKIKKAYEAKVAARNARNEARKIKNKFENRKILSGKLTPAQSKNPAIKELFLVEGDSAGGSAKLGRNRVTQAILPLRGKVINTDKAKLSDVLANEEIATIINTIGAGIGEDFNLKNAQYHKIIIMTDADTDGAHIQTLLLTFFWRYMKKLVENGMVYIALPPLYKISIKSKNTQPLYAWDEDQLKEITSKYTNYEIQRYKGLGEMNADQLWETTMNPETRSIIKVDVEDEILVERNVSTLMSDDVIARKQWINNNVDFSSEDDFKINNK" }, "paralog": false, "identity": 0.9903660886319846, "score": 1001.0, "evalue": 0.0, "description": "Internal stop codon at 683942, 684143, 685178, 685283. Nonsense mutation around 683942, 684143, 685178, 685283.", "psc": { "uniref90_id": "UniRef90_D1J8V0", "gene": "GyrB", "product": "DNA topoisomerase (ATP-hydrolyzing)", "uniref50_id": "UniRef50_Q59961", "cog_id": "COG0187", "cog_category": "L", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8V0", "UniRef:UniRef50_Q59961", "COG:COG0187", "COG:L" ] }, "pscc": { "uniref50_id": "UniRef50_Q59961", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q59961" ], "product": "DNA topoisomerase 4 subunit B" } }, "id": "GLJLFKCOHC_1178", "locus": "GCA013368035_01179" }, { "type": "cds", "sequence": "contig_1", "start": 685281, "stop": 685682, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02518.31" ], "nt": "ATGAGTTTAAATAATTACGAAGCTAGCGATTTAAAAGTTTTAAAAGGATTGGATGCTGTCAGAAAAAGACCAGGAATGTATATTGGGTCTACTGATTCAAGTGGGCTTCATCACTTAATTTGGGAAATTTTTGATAATGCTATTGATGAAGCGTTGAGTGGGTTTGCAAATAAAATAATTGTAACTTTAAAGAAGGATTCTTCTGTTATTGTTGAAGACAACGGTAGGGGAATTCCTGTCAATAAACACGAGTCTGGAAAAAGTGGAGTTGAGCTTGTCTTTACTGAACTTCATGCTGGTGGTAAATTTGGCGATGGTGCTTATAAAACAAGCGGGGGATTACACGGAGTAGGTTCCTCGGTTGTCAATGCCTTAAGTTCTAAAGTTGAAGTTACGGTTTGA", "aa": "MSLNNYEASDLKVLKGLDAVRKRPGMYIGSTDSSGLHHLIWEIFDNAIDEALSGFANKIIVTLKKDSSVIVEDNGRGIPVNKHESGKSGVELVFTELHAGGKFGDGAYKTSGGLHGVGSSVVNALSSKVEVTV", "aa_hexdigest": "5ba540597d72b901175e8134d81c6abe", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00037E1229", "query_cov": 1.0, "subject_cov": 0.8012048192771084, "identity": 0.677, "score": 187.0, "evalue": 1.9e-58, "valid": false, "product": "ATP-binding protein", "uniref50_id": "UniRef50_A0A962E3V0" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02518.31", "name": "HATPase_c", "length": 101, "aa_cov": 0.7368421052631579, "hmm_cov": 0.8514851485148515, "evalue": 1.4118490270874167e-17, "score": 59.5620231628418, "start": 32, "stop": 129 } ], "seq_stats": { "molecular_weight": 14056.700999999986, "isoelectric_point": 6.182343101501464 }, "id": "GLJLFKCOHC_1179", "locus": "GCA013368035_01180" }, { "type": "cds", "sequence": "contig_1", "start": 685736, "stop": 686194, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F47C538" ], "nt": "ATGGCTTGTTCTTTAAGCGATTTATTAGTAGTTAAATACTACTTCAACGAACCTTACGATTCAATATTTAAATCTAATAATTTTATAAAAGATTTAAGATTAATAGGTAAGGCTAAGGATTATGTAATTGACTTTGCTTTTTGCGACAAAAATGTTATAAATTCAAATAAAAAAAATATATATAAATTAATTAAAAAAGAATTCAACAACGGCAAAGATTTAACATTGATTGTAGGCCACGATACTAACATTGCTACGACCTTAGCAATGTTAGACGTCAAAATGCCAGATCTTAAACAATTAGAAAAATATCCAATTGGGTCTAAATTAATTTTTGCAATTAATGATGACAATAGTTTTGATTTGTATATTGCTTTTTTTGATTTTAAAGACATTAGAAACTTTAATCTTTCAAATCCACAAATTATAAAAATAGCAAGCAAAATTTTTCTAAAATAA", "aa": "MACSLSDLLVVKYYFNEPYDSIFKSNNFIKDLRLIGKAKDYVIDFAFCDKNVINSNKKNIYKLIKKEFNNGKDLTLIVGHDTNIATTLAMLDVKMPDLKQLEKYPIGSKLIFAINDDNSFDLYIAFFDFKDIRNFNLSNPQIIKIASKIFLK", "aa_hexdigest": "b638d06aba26115dd4e4e3235e0bdd00", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI001F47C538", "query_cov": 0.9868421052631579, "subject_cov": 0.8571428571428571, "identity": 0.5, "score": 154.0, "evalue": 3.02e-45, "valid": false, "uniref50_id": "UniRef50_UPI001F47C538" }, "pscc": { "uniref50_id": "UniRef50_UPI001F47C538", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI001F47C538" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 17583.31289999999, "isoelectric_point": 8.925406074523927 }, "id": "GLJLFKCOHC_1180", "locus": "GCA013368035_01181" }, { "type": "cds", "sequence": "contig_1", "start": 686387, "stop": 686563, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGCCCAAAAGATTAGAGAATTTTTCAATATAACAGATGATATTGATGTTAAGGTAATGGCAAATTCAACGCAAAGAACTTTTCAAACTGCGCAGTTACTTTCAATTGGCCTTTTTCCTGCTAAAAAAATCGACATTCAATGTCAAGATTATTCATTCAAAAATGAGAATGAATGA", "aa": "MAQKIREFFNITDDIDVKVMANSTQRTFQTAQLLSIGLFPAKKIDIQCQDYSFKNENE", "aa_hexdigest": "de626c0ae5f40209a4137c0b17bdc16a", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6744.595899999998, "isoelectric_point": 5.214375114440917 }, "id": "GLJLFKCOHC_1181", "locus": "GCA013368035_01182" }, { "type": "cds", "sequence": "contig_1", "start": 686838, "stop": 687095, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00044.30" ], "nt": "ATGAAAAAAACTAGAATTGCAATCAACGGTTTTGGTAGAATTGGTCGTTTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGATTTAACAGATGCAGCTACATTAGCTCACTTATTGAAATACGATACAGCTCATGGAAAAATGAACGAGAATATTTCACACACAGAAAATTCTATTGTTGTAAATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGA", "aa": "MKKTRIAINGFGRIGRLIFREIFNDPEFEIVAINDLTDAATLAHLLKYDTAHGKMNENISHTENSIVVNGKEYRVYSEKDPLNLP", "aa_hexdigest": "86d25214f487f0e534c30ff6a9ad30fb", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_UPI0024BE2B32", "query_cov": 0.9529411764705882, "subject_cov": 0.826530612244898, "identity": 0.667, "score": 114.0, "evalue": 2.02e-31, "valid": false, "product": "glyceraldehyde 3-phosphate dehydrogenase NAD-binding domain-containing protein", "uniref50_id": "UniRef50_A0A2M8L0B5" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00044.30", "name": "Gp_dh_N", "length": 81, "aa_cov": 0.9529411764705882, "hmm_cov": 1.0, "evalue": 8.488957007857683e-33, "score": 108.13509368896484, "start": 5, "stop": 85 } ], "seq_stats": { "molecular_weight": 9688.924599999997, "isoelectric_point": 6.267487907409667 }, "id": "GLJLFKCOHC_1182", "locus": "GCA013368035_01183" }, { "type": "cds", "sequence": "contig_1", "start": 687330, "stop": 687773, "strand": "+", "frame": 3, "gene": null, "product": "Glyceraldehyde-3-phosphate dehydrogenase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "nt": "TTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTCCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACTGGTGCTGCAATAGCAATTGGAAAGGTTATCCCTTCATTGGATAAAAAACTAAATGGACTATCTTTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCTACTGTTCAAGAAATTAATGAAGCAATGAAGAAATCTGCTTCTGAATCATTAGAATATTCTACTGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGTGTGTTAGAAGTAGAAGGCAAAAAACTATACAAAGTTTATGCATGA", "aa": "MEKNFGIEKGFMTTVHSYTADQRLQDAPHKDLRRARAAASNMVPTTTGAAIAIGKVIPSLDKKLNGLSLRVPTITGSFVDLTVELKKDATVQEINEAMKKSASESLEYSTEPIVSSDIIGAKAGSIFDSLLTSVLEVEGKKLYKVYA", "aa_hexdigest": "f19088dcd724609e9acacf2d9826b54d", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_D4XW52", "query_cov": 0.9251700680272109, "subject_cov": 0.8553459119496856, "identity": 0.654, "score": 172.0, "evalue": 2.24e-52, "valid": false, "product": "Glyceraldehyde 3-phosphate dehydrogenase, C-terminal domain protein", "uniref50_id": "UniRef50_A0A3B0PH09" }, "pscc": { "uniref50_id": "UniRef50_A0A3B0PH09", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3B0PH09" ], "product": "Glyceraldehyde-3-phosphate dehydrogenase" }, "genes": [], "id": "GLJLFKCOHC_1183", "locus": "GCA013368035_01184" }, { "type": "cds", "sequence": "contig_1", "start": 687869, "stop": 688357, "strand": "+", "frame": 2, "gene": null, "product": "Septicolysin", "db_xrefs": [ "RefSeq:WP_036438585.1", "SO:0001217", "UniParc:UPI000512C9D7", "UniRef:UniRef100_A0A454CAF9", "UniRef:UniRef50_D1J8V3", "UniRef:UniRef90_D1J8V3" ], "nt": "ATGAAACTTTCAATAAAAGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTTGAAGATTTAAAAAAAGAAGAATCCAAGGAAAACTTCATTGTTTATTTAGAAAACAAAAGGCCAACTAACATTGAATATGATTATCAAGATTATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAATTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATACAAAAAATATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAAAAAATTTCTAGGGTTACTACAAACGATGGCCAAAACGAATATACGGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAAATTAAACGAAGAAATTTCTGAAATTCAAATTGCAATTGATAGTGCAAACATTAATACATTAGTTGAAGTTAAGTAA", "aa": "MKLSIKEIIKKIKLLELDLEDLKKEESKENFIVYLENKRPTNIEYDYQDYSNKIKNLYNEIFKLRTLVQVTNINTITSYKKYSISELIILLAQKSNQLERLQEMRDCKKISRVTTNDGQNEYTEYLFDPKMVAIELRKLNEEISEIQIAIDSANINTLVEVK", "aa_hexdigest": "7cbe3cf54989aa1c3bfaf47a086add7e", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "ups": { "uniparc_id": "UPI000512C9D7", "ncbi_nrp_id": "WP_036438585.1", "uniref100_id": "UniRef100_A0A454CAF9", "db_xrefs": [ "SO:0001217", "UniParc:UPI000512C9D7", "RefSeq:WP_036438585.1", "UniRef:UniRef100_A0A454CAF9" ] }, "ips": { "uniref100_id": "UniRef100_A0A454CAF9", "uniref90_id": "UniRef90_D1J8V3" }, "psc": { "uniref90_id": "UniRef90_D1J8V3", "product": "Septicolysin", "uniref50_id": "UniRef50_D1J8V3" }, "pscc": { "uniref50_id": "UniRef50_D1J8V3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8V3" ], "product": "Septicolysin" }, "genes": [], "id": "GLJLFKCOHC_1184", "locus": "GCA013368035_01185" }, { "type": "cds", "sequence": "contig_1", "start": 688605, "stop": 688787, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATACAACAAAAGTTTACATAGATTTTGAAGCAATTACCAACCCTTTTGCAAGATTAGTAAATTTGCCAAGCGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGCCAATATCAAATTAAAACATTTATTATTGATTTTAAAGAACATCATAATTTAAATTCAATTTGA", "aa": "MNTTKVYIDFEAITNPFARLVNLPSGTPYAYTLGLLNIHGQYQIKTFIIDFKEHHNLNSI", "aa_hexdigest": "8990b4dae1576a3af97b68c45f302653", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6897.818599999997, "isoelectric_point": 6.8117780685424805 }, "id": "GLJLFKCOHC_1185", "locus": "GCA013368035_01186" }, { "type": "cds", "sequence": "contig_1", "start": 688766, "stop": 688987, "strand": "-", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAAAATTTTTCCTGTTAATTTTGACAATGACACAATTGTATTGGAAATTAAAGGTTCAACATTGTTTTCAGGAAATAATTTTTTTAAGCATTTATTTTCTAAAACAGGATTGTGGCCTACAAATACAACTTCCTTTAAATTTACAGTATTATTGATCTCTAATATGTCAGAAACTATTGCATTTTTTATGGCAGTTCAAATTGAATTTAAATTATGA", "aa": "MLKIFPVNFDNDTIVLEIKGSTLFSGNNFFKHLFSKTGLWPTNTTSFKFTVLLISNMSETIAFFMAVQIEFKL", "aa_hexdigest": "155cee6c432fba8a9779db58a006b694", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8372.725199999999, "isoelectric_point": 8.121356391906737 }, "id": "GLJLFKCOHC_1186", "locus": "GCA013368035_01187" }, { "type": "cds", "sequence": "contig_1", "start": 689160, "stop": 689360, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCATGACGATGTCAATAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTTTACTAAAAGAACTTTTATACAAACGTGAACTAATAAAACAAATAAAAAATTTGGAACTTGACGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGA", "aa": "MNKNLLLKELKRYSHDDVNKMLYIVSHPDETETLLKELLYKRELIKQIKNLELDDNLTIKEIKEKI", "aa_hexdigest": "c5f9e23ce5844c46f86b1d1e4514c0d0", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7996.301299999995, "isoelectric_point": 6.728338432312013 }, "id": "GLJLFKCOHC_1187", "locus": "GCA013368035_01188" }, { "type": "cds", "sequence": "contig_1", "start": 689487, "stop": 689717, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGATAATTTCATGGTTAGCCATATTGATGGTGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTCTCTATTCAAGCAATTGGAGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGATTTTGATAAAGTAAAGCAGATAATGAATTTAAGAATTTGA", "aa": "MPIIIGEIIFNLNGLLDNFMVSHIDGGIDALAYANTYTGIIYTIFFSIQAIGAMFVGQYYGRKDFDKVKQIMNLRI", "aa_hexdigest": "d3969568ee1c60af6e60a2100fd3071a", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 8568.956099999996, "isoelectric_point": 5.485781288146971 }, "id": "GLJLFKCOHC_1188", "locus": "GCA013368035_01189" }, { "type": "cds", "sequence": "contig_1", "start": 689892, "stop": 690218, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTGCTAAATGAAACTGGACATTCAAAATACGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATACGGATTTCATAAACCTGCATATTATGCTGCCTTTGGTTCAATTATTGCTGCGACAGTTTGCTTTACAAGTGATTCTTTATTTACGTATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAAATGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCTGGAATGTTAACTTTACCTACAAGAATGATTATTTGA", "aa": "MLLNETGHSKYAFVSACLTMLTNASINSICLYGFHKPAYYAAFGSIIAATVCFTSDSLFTYYKERKIFINVFKMFHITKPIAKQLLKRIPAMLIAIAGMLTLPTRMII", "aa_hexdigest": "dbe169a7b3d9239eb32e3d85b6e2c83f", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 12136.510399999983, "isoelectric_point": 9.676914024353028 }, "id": "GLJLFKCOHC_1189", "locus": "GCA013368035_01190" }, { "type": "cds", "sequence": "contig_1", "start": 690273, "stop": 690776, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCGTCCGTCGCCTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCATTTTTCGTCGCCACAAGATTAGGAGAATCTGATTTTGAAGGTGCAGATAAGCACGCCCATGCATTGCGGGGTTTTCATGCTTTGGCTGGATGTTGCATGTCTATATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTACTACAACAAAAGGTGTTGGTATCGGCGTCTCTCAGAGATTTTATGATGTGCAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAATTCACTTGGCAATGATTTTACATTCACAAAAGGTCAAAATTTGGCTTATGACCAATGGGTGGCAAGCGCAGTACTGGCAACTTCAAAAGAATTCAAAGAAACATTTTTGTTATGTAGTGCAACCTTTATTTTCTTTAATCCTATATGA", "aa": "MGINAVTILGLVESLSSVASAITSVCASNVSFFVATRLGESDFEGADKHAHALRGFHALAGCCMSILMIAVVFGIAYSPTTTKGVGIGVSQRFYDVQRKDYIIEILNKSDILKNSDLLNSLGNDFTFTKGQNLAYDQWVASAVLATSKEFKETFLLCSATFIFFNPI", "aa_hexdigest": "09ddb4df15639d1801a88b444aad005d", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 18008.497599999988, "isoelectric_point": 5.85478000640869 }, "id": "GLJLFKCOHC_1190", "locus": "GCA013368035_01191" }, { "type": "cds", "sequence": "contig_1", "start": 691148, "stop": 691453, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF00561.26" ], "nt": "ATGTTATATGATTTAGTTTCACCATATAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGCAAGCCAATTTTGTTTGTCCATGGTGGTCCAGGTGGGGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGTGGACTTAGCATACCTAGTGCCGAAATCAAACAAAATACGACCCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGA", "aa": "MLYDLVSPYNSGHLKVSDIHSIYYEESGNPNGKPILFVHGGPGGGTKPSCRQFFDPEFYRIILFDQRGCGLSIPSAEIKQNTTQDLIEDIEKLRKFLNIDK", "aa_hexdigest": "049e0eec9caeeee4c69317414b102aa2", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_A0A1L4BUW4", "query_cov": 0.9702970297029703, "subject_cov": 0.9245283018867925, "identity": 0.633, "score": 138.0, "evalue": 1.23e-40, "valid": false, "product": "Proline iminopeptidase", "uniref50_id": "UniRef50_A0A1L4BUW4" }, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF00561.26", "name": "Abhydrolase_1", "length": 71, "aa_cov": 0.6831683168316832, "hmm_cov": 0.9859154929577465, "evalue": 1.8665019052263204e-10, "score": 36.224388122558594, "start": 33, "stop": 101 } ], "seq_stats": { "molecular_weight": 11419.813099999985, "isoelectric_point": 5.849380302429199 }, "id": "GLJLFKCOHC_1191", "locus": "GCA013368035_01192" }, { "type": "cds", "sequence": "contig_1", "start": 691577, "stop": 691732, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGTATCAAAAAGGCGCTTCTTATTATTTTCCTGCTGAATATGAAGAATATTCAAACTTTATTCCTGAAAATGAAAGAGGCGATTTAATTAACGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGA", "aa": "MYQKGASYYFPAEYEEYSNFIPENERGDLINAYYKYLNSSDQNVAEQAAYH", "aa_hexdigest": "e9321f42b69b295c7098dd81562c2c79", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 6056.417700000004, "isoelectric_point": 4.375886344909667 }, "id": "GLJLFKCOHC_1192", "locus": "GCA013368035_01193" }, { "type": "cds", "sequence": "contig_1", "start": 691760, "stop": 692002, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAAAATTGCCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGCAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAAATATTTTTAAATGACGATAATTTTATTTTAAATAATGCAAGCAAGATTAAAGAAATCCCAACAATATTGATTCATGGTCAATTTGATATGGATTGTCGCCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAGTCAAAGCTTTGA", "aa": "MKKLPNLEEILSDKKSNLELARLENYYFVNKIFLNDDNFILNNASKIKEIPTILIHGQFDMDCRPVGAYLLHKALPKSKL", "aa_hexdigest": "8eff35bcba50ad8ab78d3385e415356d", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 9289.797099999992, "isoelectric_point": 8.734515190124512 }, "id": "GLJLFKCOHC_1193", "locus": "GCA013368035_01194" }, { "type": "cds", "sequence": "contig_1", "start": 692179, "stop": 692724, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF09371.15" ], "nt": "ATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCAGAAGGGGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTGTATGATGTTGAATTAAATAAAAGAAAAGAAGCCATATTGAATATATTGGCCGAAAATAAATTATTAAATGATGAATTAAAGCAAAAAATTGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAGGCATCAGATGCTATTGCTCTTGGTTTAAGTCCATTGGCTGATGAAATTTTAAATAATAGTGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGA", "aa": "MNSINNVAKKLNLSENQVKNTLSLLSEGATVPFISRYRKNITGGLDEDQITKINDLYVYDVELNKRKEAILNILAENKLLNDELKQKIEAADTKQAVENIYEPFKIGKKTKASDAIALGLSPLADEILNNSDDNYNLYYAAKKYISDKLKTEDEVLQQTKFIIAQNISQDPSTREYVKNQL", "aa_hexdigest": "32b0fc2bbce9867efdc607075b6f80b7", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF09371.15", "name": "Tex_N", "length": 73, "aa_cov": 0.40331491712707185, "hmm_cov": 1.0, "evalue": 2.016164634940162e-29, "score": 96.58173370361328, "start": 2, "stop": 74 } ], "seq_stats": { "molecular_weight": 20516.969499999996, "isoelectric_point": 5.389893913269043 }, "id": "GLJLFKCOHC_1194", "locus": "GCA013368035_01195" }, { "type": "cds", "sequence": "contig_1", "start": 693088, "stop": 693237, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF16921.10" ], "nt": "ATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCATAATCAAGAACAATGA", "aa": "MLLSPATKNKRILAIDPAYVNGCKIAAIDQNGNFLKEGIIYPHNHNQEQ", "aa_hexdigest": "738eccb456a93190e2c542b3ed3fb0da", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "pfams": [ { "id": "PF16921.10", "name": "Tex_YqgF", "length": 38, "aa_cov": 0.7755102040816326, "hmm_cov": 1.0, "evalue": 6.311783089871881e-13, "score": 44.69309997558594, "start": 10, "stop": 47 } ], "seq_stats": { "molecular_weight": 5463.2110999999995, "isoelectric_point": 7.928402519226075 }, "id": "GLJLFKCOHC_1195", "locus": "GCA013368035_01196" }, { "type": "cds", "sequence": "contig_1", "start": 693349, "stop": 694326, "strand": "+", "frame": 1, "gene": null, "product": "S1 RNA-binding domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "nt": "TTGCAAAAAAGACTTGAAAAAAATCCTAATGAAAAGATTAAGTTTGTGATTGTAAGTGAAGTTGGTGCTTCTGTTTATTCAGCTTCAAAAATTGCTCAAGAAGAATTTCCACAATTACATGTTGAATATAGATCAGCAATTCATATTGGTAGAAAGTTTCAAGACCCCTTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAAGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTTGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAATACTATAGCAAGCAATATTGTAAACTATAGAGAAGAAAACGGTTCATTTAAATCACGCGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACCATTGTCAATAAGCTAAATATAGATTTAAATAATATTGACAAAGAAAAATTATTATCAGTTGACAAAGAACAACTTGCAAAAGAATTAAATTCTGATAAATACAGTATTCAATTAATTATAGAATCATTGATTGCACCCGAAAAAGATATTAGAGATGACAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATTGATGATATAACTCAAGGTCAAATAATTAAGGGTCAAATTCAAAATGTTACCGATTTTGGAGCCTTTGCGTTTATTGGATTAAAACAAAATGTTTTGATACATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAACATACAAATAATTTCCATTGATAAAGAACACAATAAAATTCAAGGAAAAATTATTTGA", "aa": "MQKRLEKNPNEKIKFVIVSEVGASVYSASKIAQEEFPQLHVEYRSAIHIGRKFQDPLNELIKIDPKSIGIGQYQHDVNQKELSKQLTEKLNKVVNLVGVDLNTATKVILSYISGLSNTIASNIVNYREENGSFKSREELKKVKGLGPKAYEQSVGFLRIHDSNNFYDKTNIHPESYKLADTIVNKLNIDLNNIDKEKLLSVDKEQLAKELNSDKYSIQLIIESLIAPEKDIRDDKPGFLVSDKVLTIDDITQGQIIKGQIQNVTDFGAFAFIGLKQNVLIHITNMKKKENQFIKHPLDVLNVGDNVNIQIISIDKEHNKIQGKII", "aa_hexdigest": "d0b90ef213ea410aacde4e8011e64be0", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI003593DDCE", "query_cov": 0.8615384615384616, "subject_cov": 0.9929078014184397, "identity": 0.7390000000000001, "score": 412.0, "evalue": 1.23e-142, "valid": false, "product": "helix-hairpin-helix domain-containing protein", "uniref50_id": "UniRef50_A0A502M9J9" }, "pscc": { "uniref50_id": "UniRef50_A0A502M9J9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A502M9J9" ], "product": "S1 RNA-binding domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_1196", "locus": "GCA013368035_01197" }, { "type": "cds", "sequence": "contig_1", "start": 694553, "stop": 695380, "strand": "+", "frame": 2, "gene": null, "product": "site-specific DNA-methyltransferase (adenine-specific)", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "nt": "ATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAACGTCAAGCAGGAATTGAAGACTTTGATTATACAATTTTATCAGATGAAGAAGCATTGACGGGCAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAAAAATGCTACAACAAATAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAGGGGCAACAAAGTGAAAATGATTTTTCAGGTTTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGTAATTACCAAGATAATTCAATAGATGCCTTTGGTGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCCGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTTAATAAGAAAAAAGTTAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTGCTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGA", "aa": "MFYRYISENIATYANNRQRQAGIEDFDYTILSDEEALTGRDDLINEKGFFILPSELFINVVKNATTNNCLNETLDNIFKNIESSAKGQQSENDFSGLFNDVDVNSQKLGRSVDERNKKLAAILQEIAAMKLGNYQDNSIDAFGDAYEYLMSMYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIK", "aa_hexdigest": "558f051a7301bdb7f08055161e1a18b2", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0028EAAB7E", "query_cov": 1.0, "subject_cov": 0.8136094674556213, "identity": 0.6579999999999999, "score": 369.0, "evalue": 1.91e-125, "valid": false, "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7U9ST90", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ] }, "pscc": { "uniref50_id": "UniRef50_A0A7U9ST90", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A7U9ST90" ], "product": "site-specific DNA-methyltransferase (adenine-specific)" }, "genes": [], "id": "GLJLFKCOHC_1197", "locus": "GCA013368035_01198" }, { "type": "cds", "sequence": "contig_1", "start": 695537, "stop": 696001, "strand": "+", "frame": 2, "gene": null, "product": "type I restriction-modification system subunit M", "db_xrefs": [ "EC:2.1.1.72", "GO:0003677", "GO:0009007", "GO:0009307", "KEGG:K03427", "SO:0001217", "UniRef:UniRef50_A0A7X6YKI2", "UniRef:UniRef90_UPI001939D1E0" ], "nt": "ATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAGGCAACTTGTTTTTTGGAACAAGCATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATCGACAATAATATCTTATTTATTGATGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAATTAACTAGTCAAAATATTGACAATATTATTGATTATTATGGTCAAAGAAAAGACATCTCTTATATTTCAAAATTAGCCAATGTTGAAGAAATCAAGCCCAATTCATATAATTTATCAGTCAATAGTTATGTTGAAAAACAAGACACTTCAGAAAAAATAGATATCAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGTGAAATTGACAAAATAGTTGCTGAATTAGAGGAATAG", "aa": "MYRGGAEQKIRQYLVENNFIDAIIQLPGNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNIDNIIDYYGQRKDISYISKLANVEEIKPNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE", "aa_hexdigest": "57a280fcb329d2abc51d53a6e14b73da", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_R6V7S9", "query_cov": 0.9935064935064936, "subject_cov": 0.9807692307692307, "identity": 0.654, "score": 201.0, "evalue": 7.37e-64, "valid": false, "product": "site-specific DNA-methyltransferase (adenine-specific)", "uniref50_id": "UniRef50_A0A645HPD1" }, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 695237, "stop": 696001, "observations": { "mutation": [ 695378, 695489 ], "stop": [ 695378, 695489 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI001939D1E0", "query_cov": 1.0, "subject_cov": 0.4652567975830816, "identity": 0.968, "score": 278.0, "evalue": 1.22e-91, "gene_start": 1, "gene_end": 154, "reference_start": 178, "reference_end": 331, "reference_sequence": "MYASNAGKSGGEYFTPQEVSELLTKIAVFNKKKVNRVYDPACGSGSLLLQTIKVLGKENIKDGFYGQEVNLTTYNLCRINMFLHDIGFDKFNIYNGDTLLSPSPEHQRKEPFDVIVSNPPYSIKWEGEDNPLLINDQRFSPAGILAPKSKADFAFILHSLSWLATDGVAAIVCFPGIMYRGGAEQKIRQYLVENNFIDAIIQLPSNLFFGTSISTCIMVLKKSKIDNNILFIDASQEFLKVTNNNKLTSQNINNIIDYYGQWKDISYISKLASVEEIKSNSYNLSVNSYVEKQDTSEKIDINVLNSQIKETVSKIETLRSEIDKIVAELEE" }, "paralog": false, "identity": 0.9724409448818898, "score": 482.0, "evalue": 1.21e-174, "description": "Internal stop codon at 695378, 695489. Nonsense mutation around 695378, 695489.", "psc": { "uniref90_id": "UniRef90_UPI001939D1E0", "product": "type I restriction-modification system subunit M", "ec_ids": [ "2.1.1.72" ], "uniref50_id": "UniRef50_A0A7X6YKI2", "kegg_orthology_id": "K03427", "go_ids": [ "GO:0003677", "GO:0009007", "GO:0009307" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI001939D1E0", "EC:2.1.1.72", "UniRef:UniRef50_A0A7X6YKI2", "KEGG:K03427", "GO:0003677", "GO:0009007", "GO:0009307" ] } }, "id": "GLJLFKCOHC_1198", "locus": "GCA013368035_01199" }, { "type": "cds", "sequence": "contig_1", "start": 696463, "stop": 696732, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E04BC5F" ], "nt": "ATGGAAAATATTATCGAGTTGCAAATTCCACTTCCCCCTCTTGAAATCCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAAATAAACAATATAATTACTATAGAAATAAATTATTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAAACAACAATGCAGTAATAGTATAGTTGAATATAAGAGGTTATGA", "aa": "MENIIELQIPLPPLEIQNQIVNILDKFTELTTELTTELTTELTTELTYRNKQYNYYRNKLLDFDNNKELLNKIMNKQQCSNSIVEYKRL", "aa_hexdigest": "df0600436dc8e6404b30e6f1ec1bbf48", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI0034D97483", "query_cov": 0.9325842696629213, "subject_cov": 1.0121951219512195, "identity": 0.867, "score": 115.0, "evalue": 3.66e-32, "valid": false, "product": "restriction endonuclease subunit S", "uniref50_id": "UniRef50_UPI000E04BC5F" }, "pscc": { "uniref50_id": "UniRef50_UPI000E04BC5F", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI000E04BC5F" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "GLJLFKCOHC_1199", "locus": "GCA013368035_01200" }, { "type": "cds", "sequence": "contig_1", "start": 696793, "stop": 697329, "strand": "+", "frame": 1, "gene": null, "product": "restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_UPI00131480C8", "UniRef:UniRef90_UPI00131480C8" ], "nt": "TTGTCATTTAAGCATGTTTCTGCAAATGAACTTAAGCTCTATAAAAAAGAAGAAACGGGAAATGTAAAATTATTATCAACAGGTCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTACGGTGAGATTATTTCAATACCTTCTGGTGGCGCGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTATTTTTTAATTGCTAGTAAGGAAAACATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATTGAATTACAAATTCCACTTCCTTCTCTTGAAATCCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACAAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGATCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGGTATTAA", "aa": "MSFKHVSANELKLYKKEETGNVKLLSTGQYDGFTSYVENDKNINYGEIISIPSGGAPLIKYYKGYFIDSLNILLSSKDVKKYSLKFLYYFLIASKENIEQNFRGSSIKHPDMENIIELQIPLPSLEIQNKIVEILDKLETYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGGY", "aa_hexdigest": "6fe8f119cd81bd2a3b43c95e56fdc613", "start_type": "TTG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_UPI00131480C8", "query_cov": 0.9887640449438202, "subject_cov": 0.8036529680365296, "identity": 0.9940000000000001, "score": 350.0, "evalue": 1.31e-121, "valid": true, "product": "restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_UPI00131480C8", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_UPI00131480C8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_UPI00131480C8" ], "product": "restriction endonuclease subunit S" }, "genes": [], "id": "GLJLFKCOHC_1200", "locus": "GCA013368035_01201" }, { "type": "cds", "sequence": "contig_1", "start": 697737, "stop": 697967, "strand": "+", "frame": 3, "gene": null, "product": "Type I restriction modification DNA specificity domain-containing protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9NTY9" ], "nt": "ATGTTACAAAATTTTGTTATCCCCATTCCCCACATTTCAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAATCTGGACTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGACAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGA", "aa": "MLQNFVIPIPHISIQNKIVEILDKLQAYTKDIQSGLPLEIDQRKKQYEYYRDKLLDFKDLAGGVLSKNYLLLLNEL", "aa_hexdigest": "258d6c1a3cf425456a5f3c661b8cfd6a", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_F9NTY9", "query_cov": 0.8026315789473685, "subject_cov": 0.8356164383561644, "identity": 0.557, "score": 69.3, "evalue": 4.63e-14, "valid": false, "product": "Type I restriction modification DNA specificity domain-containing protein", "uniref50_id": "UniRef50_F9NTY9" }, "pscc": { "uniref50_id": "UniRef50_F9NTY9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_F9NTY9" ], "product": "Type I restriction modification DNA specificity domain-containing protein" }, "genes": [], "id": "GLJLFKCOHC_1201", "locus": "GCA013368035_01202" }, { "type": "cds", "sequence": "contig_1", "start": 698061, "stop": 698528, "strand": "+", "frame": 3, "gene": null, "product": "Restriction endonuclease subunit S", "db_xrefs": [ "EC:3.1.21.3", "KEGG:K01154", "SO:0001217", "UniRef:UniRef50_A0A454CAF2", "UniRef:UniRef90_A0A454CAF2" ], "nt": "ATGAACAAAGGGGATTATTTAGCAATAACTTCAGGAAATAAATCATTTTATGTCTCTGATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGTGGGCAAGCAGACGTTAAATATAATCAAGGAAAAACAATGTATTCCGATCATGTGTTTGCGTTTAAAACAAATGAATGCAACACAAAATATCTTTACTTTTATTTATTGAATATTTCTAATTTTATTAACGAAAAATTATTTGTTGGTAGTGGATTAAAAAATTTAAATAAAAAAGAATTCTTGAATCTTAATATTGCAATTCCTCCTATTGAAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAAACTGGTCTTCCTCTTGAAATTGACCAACGTAAAAAGCAATACGAACATTACAGGGATAAATTATTGAACTTTAAAGAAAAGAATACTAAAATTTAA", "aa": "MNKGDYLAITSGNKSFYVSDFLVDGEYIFVNDGGQADVKYNQGKTMYSDHVFAFKTNECNTKYLYFYLLNISNFINEKLFVGSGLKNLNKKEFLNLNIAIPPIEIQNKIVEILDKLQAYTKDIQTGLPLEIDQRKKQYEHYRDKLLNFKEKNTKI", "aa_hexdigest": "bc26592f60119368824511b8924b14e1", "start_type": "ATG", "rbs_motif": null, "psc": { "uniref90_id": "UniRef90_A0A454CAF2", "query_cov": 1.0, "subject_cov": 1.0, "identity": 0.987, "score": 305.0, "evalue": 3.75e-105, "valid": true, "product": "Restriction endonuclease subunit S", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A454CAF2", "kegg_orthology_id": "K01154" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF2" ], "product": "Restriction endonuclease subunit S" }, "genes": [], "id": "GLJLFKCOHC_1202", "locus": "GCA013368035_01203" }, { "type": "cds", "sequence": "contig_1", "start": 698560, "stop": 698799, "strand": "+", "frame": 1, "gene": null, "product": "Type I restriction-modification system, restriction subunit R", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "nt": "ATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACAGTTGTTTCTGAATATAAGCCTCTAGCGAATAAAGCAAAAGATTATCAAACAGAGAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAACCAAGGATATGAGTATTTGAACATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGTTAAATAAAATAAATTTTTCAGATAATGAATGA", "aa": "MEKYNIIVENENDTVVSEYKPLANKAKDYQTESELESQFISMLKNQGYEYLNIHNEEDLINNLRQQIELLNKINFSDNE", "aa_hexdigest": "cc1b22fe18b7ae4c95a225b4c13a441b", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_A0A0F5H0F8", "query_cov": 1.0379746835443038, "subject_cov": 1.0, "identity": 0.524, "score": 71.2, "evalue": 1.14e-14, "valid": false, "product": "Type I restriction-modification system, restriction subunit R", "uniref50_id": "UniRef50_A0A0F5H0F8" }, "pscc": { "uniref50_id": "UniRef50_A0A0F5H0F8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A0F5H0F8" ], "product": "Type I restriction-modification system, restriction subunit R" }, "genes": [], "id": "GLJLFKCOHC_1203", "locus": "GCA013368035_01204" }, { "type": "cds", "sequence": "contig_1", "start": 699022, "stop": 699285, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGGTTAATGGCATTCCAATGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTCTAAAAGAGGCTTTTAATCAAATCATTAGATATCAAAGAGATTCGTTTTGGGCTAGCAACGGCTTATATGAATATGTACAAGTGTTTGTTATTTCAAACGGGGCAAATACCAAATATTATTCAAATACAACAAGATTACAACATGTTAATAAAAAATCAGATATGATAGATTCAAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGA", "aa": "MVNGIPMVHIELKRRGVALKEAFNQIIRYQRDSFWASNGLYEYVQVFVISNGANTKYYSNTTRLQHVNKKSDMIDSKTSKSFEFTHF", "aa_hexdigest": "c5fc9c08793e399412bd90854a98a26c", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 10196.490599999994, "isoelectric_point": 9.695351982116701 }, "id": "GLJLFKCOHC_1204", "locus": "GCA013368035_01205" }, { "type": "cds", "sequence": "contig_1", "start": 699367, "stop": 699516, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "TTGACCAAATATTGTATATTTACATCTGAAGAAATGTTGCTAGTAATGAGACCTTATCAAATTGTTGCTGTTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCGGGTGGCTTTATTTGA", "aa": "MTKYCIFTSEEMLLVMRPYQIVAVEKIINKINISHNYKKYGSIAAGGFI", "aa_hexdigest": "7ec3749f931cb3c8345612fd2e48a881", "start_type": "TTG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 5627.6636, "isoelectric_point": 9.251745033264157 }, "id": "GLJLFKCOHC_1205", "locus": "GCA013368035_01206" }, { "type": "cds", "sequence": "contig_1", "start": 699541, "stop": 700671, "strand": "+", "frame": 1, "gene": null, "product": "DEAD/DEAH box helicase", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "nt": "TTGACCTCTTTTAAAACTGCAAAATTAGCCTCACAATTGCCTTATATAAATAAAGTAATTTTTGTTGTTGATAGAAGAGATTTAGACTATCAAACAATGAAAGAATATGACAAATTAGAAAAAGGCGCTGCAAATTCAAATCGTTCTACATTAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCTGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTTCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCAGAAGTGATTAAAACAACCGAACAAGCTTTCGGCCAAAAATTGCACACTTATACAATAATAAATGCAATAAATGATAGAAACGTTTTGCCATTTAAAATTGAATATATTAAAACAATTGATGTTGATAAATCGCTAAACGATAAAGAGGATCAAAAAGTAAAAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTTGATCAAAAAACTAGGAGAAATTCCAATATAATTTATAGCCATTCAATATCTGATAATTTAGATAAAACTATTAAAAGAAATGTGAGCGGTTTTAATTCTATTATGGCGGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAATCAACTTGCTCAAGACCCTAAAAACAATCTAAAAATTGCTTTAATATATTCATATGGGGTTAATACCGAGGAATCATATGGCGGTTTAATCGATGAAGAGGATAATGAAAATACATTGCAATTAAGTAAGCCAGATAGAGATTTTTTGGATGGTGCTATTAAAGATTATAATAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTAGACATGCTGATTGTAGTAAACATGTTTTTAACTGGTTTTGACGCCCCAACATTAAATACATTATGA", "aa": "MTSFKTAKLASQLPYINKVIFVVDRRDLDYQTMKEYDKLEKGAANSNRSTLILQRQLESNDPNKKIIITTIQKLSIFIKNNRNHPVFDKEVVLIFDECHRSQFGSMHTAVIKNFKKYYLFGFTGTPIFAANINKSSPEVIKTTEQAFGQKLHTYTIINAINDRNVLPFKIEYIKTIDVDKSLNDKEDQKVKDIDRESVFLDQRRIKLVVEYILKHFDQKTRRNSNIIYSHSISDNLDKTIKRNVSGFNSIMAVSSIEMAKKYYLEFKNQLAQDPKNNLKIALIYSYGVNTEESYGGLIDEEDNENTLQLSKPDRDFLDGAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTL", "aa_hexdigest": "0ca0ba012ee67db561edb524466272a0", "start_type": "TTG", "rbs_motif": "AGGA/GGAG/GAGG", "psc": { "uniref90_id": "UniRef90_UPI0030771B9D", "query_cov": 1.0265957446808511, "subject_cov": 0.9578163771712159, "identity": 0.591, "score": 433.0, "evalue": 5.16e-148, "valid": false, "product": "HsdR family type I site-specific deoxyribonuclease", "ec_ids": [ "3.1.21.3" ], "uniref50_id": "UniRef50_A0A3E2CM26", "kegg_orthology_id": "K01153" }, "pscc": { "uniref50_id": "UniRef50_A0A3E2CM26", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A3E2CM26" ], "product": "DEAD/DEAH box helicase" }, "genes": [], "id": "GLJLFKCOHC_1206", "locus": "GCA013368035_01207" }, { "type": "cds", "sequence": "contig_1", "start": 700996, "stop": 701643, "strand": "+", "frame": 1, "gene": null, "product": "type I restriction endonuclease subunit R, EcoR124 family", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6", "UniRef:UniRef90_UPI00280A69CD" ], "nt": "TTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGACGAATTCATTGGTAAAGAAATTTTAAATGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACTGAATTATTAAAGACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTTAGAGGAAAGACAATTACTGAAATTAACAATTTAAGAAATGAAATATCTTTAGCTTCTTCTTCATCGCCTGAATTAAGAAGTAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACAAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAAAAGAAAGAGCTTGACAAAATTATTAGTGAAGAAAATTTGGATTTTGATAAAACCCATAAATTTATGGATGATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCGTTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGGCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAA", "aa": "MFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDDSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI", "aa_hexdigest": "dc00a72271e9cca2ee211ea66ebf6ae9", "start_type": "TTG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 700696, "stop": 701643, "observations": { "mutation": [ 700954 ], "stop": [ 700954 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_UPI00280A69CD", "query_cov": 1.0, "subject_cov": 0.5456852791878173, "identity": 0.991, "score": 402.0, "evalue": 7.18e-139, "gene_start": 1, "gene_end": 215, "reference_start": 180, "reference_end": 394, "reference_sequence": "MQLNKPDRDFLDGAIKDYNNMFNTNFNSEGEQFQMYYKDVSLKLKNKDLDMLIVVNMFLTGFDAPTLNTLWVDKKLREHGLIQAFSRTNRILNDKKVYGNIVCFRNLEKETDQAISIFGDGEASGIVKIKNFVDYYYGYNDSQQVHHNGYCDYIQKLNNEFPLDNWNVISEQKNKEFIILFGKVLKLKNILASFDEFIGKEILNVRDFQNYSSKYLDIKDEFKKIYKEEIASINDDVIFETELLKTYEVNIDYILQKVEEFRGKTITEINNLRNEISLASSSSPELRSKKQLIEKFVSTINNNEQDIPTKFQEFMELEKKKELDKIISEENLDFDKTHKFMDNSFELGEIKTAGSEIGDLLPKMPLFGSKGKERSIKKKAVLEKFENYFQKFYI" }, "paralog": false, "identity": 0.9936507936507937, "score": 620.0, "evalue": 4.25e-227, "description": "Internal stop codon at 700954. Nonsense mutation around 700954.", "psc": { "uniref90_id": "UniRef90_UPI00280A69CD", "product": "type I restriction endonuclease subunit R, EcoR124 family", "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_UPI00280A69CD", "UniRef:UniRef50_A0A412PBS6" ] }, "pscc": { "uniref50_id": "UniRef50_A0A412PBS6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A412PBS6" ], "product": "Type I restriction endonuclease subunit R" } }, "id": "GLJLFKCOHC_1207", "locus": "GCA013368035_01208" }, { "type": "cds", "sequence": "contig_1", "start": 701707, "stop": 702333, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF08529.16" ], "nt": "ATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAAGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGACGAAGAAGCAGATCTTGAATTTATTTTCGATCAAGAAAATGACAATTTTGCAATTATTAATCATAATAAAGTTGTCGTTGGCGATCCAATAACAAGTGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCGTTAAGTGATGCTAAAAAAATAGATCCAAATGCAGAAATTGGAGATTCCCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGACATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGTGAAGTCGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGATGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAACCAAAGAATAATTTCAAAATTAAATCCAGGCGATTGA", "aa": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNAEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGD", "aa_hexdigest": "4f9281516ccb8135e0fea36fb62d02dd", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF08529.16", "name": "NusA_N", "length": 130, "aa_cov": 0.625, "hmm_cov": 0.9153846153846154, "evalue": 4.902407938446978e-21, "score": 70.60208892822266, "start": 24, "stop": 153 } ], "seq_stats": { "molecular_weight": 23765.912400000016, "isoelectric_point": 5.478733253479004 }, "id": "GLJLFKCOHC_1208", "locus": "GCA013368035_01209" }, { "type": "cds", "sequence": "contig_1", "start": 702541, "stop": 703347, "strand": "+", "frame": 1, "gene": "nusA", "product": "Transcription termination/antitermination protein NusA", "db_xrefs": [ "COG:COG0195", "COG:K", "SO:0001217", "UniRef:UniRef50_D1J8W2", "UniRef:UniRef90_D1J8W2" ], "nt": "ATGGAAGAAATTGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGTGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGTATATATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGTAATATTACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTGGCAATAAAAATGCTACAAACAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTACGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAA", "aa": "MEEIGAIMGRDSERISTISRKLNGEKIDVVLYSDDIKVYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNATNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW", "aa_hexdigest": "a4d1094e9bfcff61b52d325cb2409c1c", "start_type": "ATG", "rbs_motif": null, "genes": [ "nusA" ], "truncated": "5-prime", "pseudogene": { "start": 702241, "stop": 703347, "observations": { "mutation": [ 702331 ], "stop": [ 702331 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W2", "query_cov": 1.0, "subject_cov": 0.4908424908424908, "identity": 0.9890000000000001, "score": 498.0, "evalue": 1.55e-173, "gene_start": 1, "gene_end": 268, "reference_start": 279, "reference_end": 546, "reference_sequence": "MPPFLLKIDKKGMKMSEIKLTAKEIYKQIYNLSQIKKIDQAEVVEAFKNTVTKLITETYDEEADLEFIFDQENDNFAIINHNKVVVGDPITSEEKDRLTRCIEIPLSDAKKIDPNAEIGDSLSQEINFEYFSKKDYNKILANFSQDIKNLERKMTCEKYASEVGNSTKAKIVSVNKGKVNLELRDGTLAFMPSNFVNQRIISKLNPGDWIDVVIEEVKEENSNAQIIVSSVESRLLQKLFEIEIPEISQGLISIVNIARIPGERAKVSIKKSNDAPETMEEIGAIMGRDSERISTISKKLNGEKIDVVLYSDDIKEYIKNALSPAKVIDLIEVPSKNSYPSFNVIVPTIQHTLAIGKKGQNVSLASELVKAKLDILSQEQADERGIEYNFENGNITKEEIELLESGKKLHHSFKHPRTIGNKNASNKFNTFENSFNIDEFDEDLAEMRRKAQQNDNIFERQMFSNSLDEDLERTLSELNDDKENNVDENDLDPYSKSFDELNEQKNDEEEDYEKITATKMKDFKRDEDLSSGLENIDLSDLDDEKW" }, "paralog": false, "identity": 0.9864130434782609, "score": 706.0, "evalue": 1.45e-257, "description": "Internal stop codon at 702331. Nonsense mutation around 702331.", "psc": { "uniref90_id": "UniRef90_D1J8W2", "gene": "NusA", "product": "Transcription termination/antitermination protein NusA", "uniref50_id": "UniRef50_D1J8W2", "cog_id": "COG0195", "cog_category": "K", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W2", "UniRef:UniRef50_D1J8W2", "COG:COG0195", "COG:K" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8W2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W2" ], "product": "Transcription termination/antitermination protein NusA" } }, "id": "GLJLFKCOHC_1209", "locus": "GCA013368035_01210" }, { "type": "cds", "sequence": "contig_1", "start": 703341, "stop": 703598, "strand": "+", "frame": 3, "gene": "ylxR", "product": "YlxR domain-containing protein", "db_xrefs": [ "COG:AJ", "COG:COG2740", "KEGG:K07742", "SO:0001217", "UniRef:UniRef50_D1J8W3", "UniRef:UniRef90_D1J8W3" ], "nt": "ATGGTAAAGTCTATACAAAGAAAGTCAATTGTTGATAATAAAATATATGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTCTAAACGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAGAATATAGATATTCAAAAATACAATGAAATAAGGAAAGAGGTGGATGAATGA", "aa": "MVKSIQRKSIVDNKIYDIDLLLRFSKDKNNLISFDPYKKNPGRGAYCLNDSEQIEILFKRRLLNKAFKQNIDIQKYNEIRKEVDE", "aa_hexdigest": "50e290183594512d6394b48159039ae9", "start_type": "ATG", "rbs_motif": "GGA/GAG/AGG", "psc": { "uniref90_id": "UniRef90_D1J8W3", "query_cov": 1.0, "subject_cov": 0.85, "identity": 0.988, "score": 168.0, "evalue": 9.64e-53, "valid": true, "gene": "YlxR", "product": "YlxR domain-containing protein", "uniref50_id": "UniRef50_D1J8W3", "cog_id": "COG2740", "cog_category": "AJ", "kegg_orthology_id": "K07742" }, "pscc": { "uniref50_id": "UniRef50_D1J8W3", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8W3" ], "product": "YlxR domain-containing protein" }, "genes": [ "ylxR" ], "id": "GLJLFKCOHC_1210", "locus": "GCA013368035_01211" }, { "type": "cds", "sequence": "contig_1", "start": 703595, "stop": 704338, "strand": "+", "frame": 2, "gene": null, "product": "Translation initiation factor IF-2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "nt": "ATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCATTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCAAAATTTTCTCTTAAAATAAATAAAAACCCTACTGAAATATTGACCTATTATTTTAAAAAGGGAATAATCAAAACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGACCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGACATGTTGACCATGGTAAGACTACATTAATTGATAGAATAAGACATAGTAATGTTACTGAAACAGAATTCGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACATTCTTAGATACACCTGGTCATGAGGCTTTTACTGAAATGCGTTCAAGGGGAGCAAAAATTACTGATATAGTAGTCATTGTAGTTGCGGCAGATGATGGAGTTAAACCTCAAACTATAGAAGCAATAGACCATGCAAAAGCAGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAAGGAGCCGATATTGAAAGAATAAAAAGCGAACTTGCTGATAACAATGTTTTATGTGAAGAATGA", "aa": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISKFSLKINKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEE", "aa_hexdigest": "2c9c91fdc1921c42aa2fa534e582e2ff", "start_type": "ATG", "rbs_motif": "AGxAGG/AGGxGG", "psc": { "uniref90_id": "UniRef90_UPI0025CBB306", "query_cov": 1.0242914979757085, "subject_cov": 0.8322368421052632, "identity": 0.534, "score": 262.0, "evalue": 3.17e-84, "valid": false, "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_A0A357QY70", "kegg_orthology_id": "K02519" }, "pscc": { "uniref50_id": "UniRef50_A0A357QY70", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A357QY70" ], "product": "Translation initiation factor IF-2" }, "genes": [], "id": "GLJLFKCOHC_1211", "locus": "GCA013368035_01212" }, { "type": "cds", "sequence": "contig_1", "start": 704537, "stop": 705412, "strand": "+", "frame": 2, "gene": "infB", "product": "translation initiation factor IF-2", "db_xrefs": [ "COG:COG0532", "COG:J", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413", "KEGG:K02519", "SO:0001217", "UniRef:UniRef50_Q98R05", "UniRef:UniRef90_D1J8W4" ], "nt": "TTGCTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTCTAACCTCTATTGATGGTAAGCCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATTACTGGTTTAAGCAAAAACCCTCAATCGGGTGATAAATTTGTAGCTATTAGTGATGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCGTATTTAGATAAATGTAAAGAATTAAATGAAAAAAATGCATTTTCTATACAAGATGGAATAAAAACTGTAAATGTCATTGTTAAGACCGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATCAGAAATGATGAAATTGCAGTTAACGTTATAAGAGCAACCTCTGGCGAAGTAACGAAAGCAGATATCTTGCTTGCTGAAACATCTAATGCAAGAATTTATTTATTCAACAATACAATAAACAATGAAATTAAAAAGGCTGCCGAATTAAAGAATATAAAAATCTTTAAACATAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTATGAAGAAAAACTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGCCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAAGTATATAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAGGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAA", "aa": "MLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN", "aa_hexdigest": "25f5765bf5fa470359b78f9f0bffe737", "start_type": "TTG", "rbs_motif": null, "genes": [ "infB" ], "truncated": "5-prime", "pseudogene": { "start": 704237, "stop": 705412, "observations": { "mutation": [ 704336 ], "stop": [ 704336 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W4", "query_cov": 1.0, "subject_cov": 0.4809917355371901, "identity": 0.997, "score": 557.0, "evalue": 1.69e-195, "gene_start": 1, "gene_end": 291, "reference_start": 315, "reference_end": 605, "reference_sequence": "MSKQNIQKRKSNVSSLKEQLTAGETKLQDGVFIFNGPLTISEFSLKISKNPTEILTYYFKKGIIKTINTTLSEDEIAELCFEFNLDFKKEENVDAQNLFNKLEIVEDPNDLASRPPIVTIMGHVDHGKTTLIDRIRHSNVTETEFGGITQHTGAYQVEYQGRKITFLDTPGHEAFTEMRSRGAKITDIVVIVVAADDGVKPQTIEAIDHAKAANVPIIVFVNKMDKQGADIERIKSELADNNVLCEEWGGNYQFVYGSALKNSNINELLEAIFLQADLLDLKANINRDPIGTIIESRIDKGRGVVSTVIVESGTLLPRDFIVAGGQYGKIRSLTSIDGKPIQKALPGTPCIITGLSKNPQSGDKFVAISDEKFAKKLASEKSYLDKCKELNEKNAFSIQDGIKTVNVIVKTDVQGTAEAIKQTLQKIRNDEIAVNVIRATSGEVTKADILLAETSNARIYLFNNTINNEIKKAAELKNIKIFKHNIIYKMVEELETLITTLKEPVYEEKLIGSATVLQLFFYSKIGQIAGCMMQEGIVKEFSKVEVYRRNKLIFKGQIISLKRGKNDVKSVEKGFDFGTHVKDFDKIEVDDELKFYEDVLVNEHN" }, "paralog": false, "identity": 0.9974424552429667, "score": 755.0, "evalue": 7.26e-276, "description": "Internal stop codon at 704336. Nonsense mutation around 704336.", "psc": { "uniref90_id": "UniRef90_D1J8W4", "gene": "infB", "product": "translation initiation factor IF-2", "uniref50_id": "UniRef50_Q98R05", "cog_id": "COG0532", "cog_category": "J", "kegg_orthology_id": "K02519", "go_ids": [ "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W4", "UniRef:UniRef50_Q98R05", "COG:COG0532", "COG:J", "KEGG:K02519", "GO:0003743", "GO:0003924", "GO:0005525", "GO:0005737", "GO:0006413" ] }, "pscc": { "uniref50_id": "UniRef50_Q98R05", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98R05" ], "product": "Translation initiation factor IF-2" } }, "id": "GLJLFKCOHC_1212", "locus": "GCA013368035_01213" }, { "type": "cds", "sequence": "contig_1", "start": 705399, "stop": 705632, "strand": "+", "frame": 3, "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "db_xrefs": [ "COG:COG0858", "COG:J", "GO:0006364", "SO:0001217", "UniRef:UniRef50_A0A448ZXY5", "UniRef:UniRef90_D1J8W5" ], "nt": "ATGAACACAATTAATCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATATTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTAGCAAAGAATTGA", "aa": "MNTINHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKN", "aa_hexdigest": "f25d477706714015a6172cd1e1eb10bd", "start_type": "ATG", "rbs_motif": "AGGA/GGAG/GAGG", "genes": [ "rbfA" ], "truncated": "3-prime", "pseudogene": { "start": 705399, "stop": 705740, "observations": { "mutation": [ 705630 ], "stop": [ 705630 ], "directions": [ "3-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W5", "query_cov": 1.0, "subject_cov": 0.6814159292035398, "identity": 1.0, "score": 154.0, "evalue": 2.25e-47, "gene_start": 1, "gene_end": 77, "reference_start": 1, "reference_end": 77, "reference_sequence": "MNTINHERKTQLLLELIGNSFYELKDFDVTNISINDVILSNDGSHAKIYVTLFKDQKRYLEKLTTMTPFIRSVVAKNWKYKVLPELIFEIDTVEEHAAKIDKILNKIEEEKNN" }, "paralog": false, "identity": 0.9911504424778761, "score": 220.0, "evalue": 7.88e-76, "description": "Internal stop codon at 705630. Nonsense mutation around 705630.", "psc": { "uniref90_id": "UniRef90_D1J8W5", "gene": "rbfA", "product": "30S ribosome-binding factor RbfA", "uniref50_id": "UniRef50_A0A448ZXY5", "cog_id": "COG0858", "cog_category": "J", "go_ids": [ "GO:0006364" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W5", "UniRef:UniRef50_A0A448ZXY5", "COG:COG0858", "COG:J", "GO:0006364" ] }, "pscc": { "uniref50_id": "UniRef50_A0A448ZXY5", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A448ZXY5" ], "product": "Ribosome-binding factor A" } }, "id": "GLJLFKCOHC_1213", "locus": "GCA013368035_01214" }, { "type": "cds", "sequence": "contig_1", "start": 705761, "stop": 706033, "strand": "+", "frame": 2, "gene": null, "product": "Glucose-inhibited division protein A", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "nt": "ATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGTGGTTCTGCAAAAGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGTTAAATACATCTAAAGGCCCAGCAGTTTGA", "aa": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAV", "aa_hexdigest": "1a1f7e54074d6f0611af994f07d089c1", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_UPI0021AE77E7", "query_cov": 0.9555555555555556, "subject_cov": 0.8113207547169812, "identity": 0.733, "score": 130.0, "evalue": 8.79e-38, "valid": false, "product": "FAD-dependent oxidoreductase", "uniref50_id": "UniRef50_A0A1T4L5B9" }, "pscc": { "uniref50_id": "UniRef50_A0A1T4L5B9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A1T4L5B9" ], "product": "Glucose-inhibited division protein A" }, "genes": [], "id": "GLJLFKCOHC_1214", "locus": "GCA013368035_01215" }, { "type": "cds", "sequence": "contig_1", "start": 706112, "stop": 707440, "strand": "+", "frame": 2, "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "db_xrefs": [ "COG:COG0445", "COG:J", "GO:0002098", "GO:0008033", "KEGG:K03495", "SO:0001217", "UniRef:UniRef50_Q98QV8", "UniRef:UniRef90_D1J8W6" ], "nt": "TTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGTGTTGGAATTGAAACACTTGAACATGGTAAACTATATTCAAAAGTTGTTATTATGACAACTGGTGTTTATATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCCGATGGAGAAAAGACAAGTTCAACACTTTCAAAAAATTTAAAAAAATATGGTTTTGAAATAATAAGATTAAAAACTGGAACACCTTGCAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTAGAAAAAAATGAACTTTGTTTTTCAAGCCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTCGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAACCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCAGCTCAAGGATTAATAGCAGGAATTAACGCTGCTAATAAACTTGATAATTTAGAACCATTAATCATAAAAAGAAATGACGGTTATATTGGCGTTTTAATTGATGACATTGTAACTAAAGGCACAAATGAACCTTATAGAATGTTAACAAGCAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAAGCAAAGAAGAACATGATAGGATTGAAGAAAAATATAAGATTATTGCTGAAACAGTAGAAAAATTAAAAAATGAATATGTTTCTTCTAAAAGTGATGTTGGCCAAAAGTACAATGTAACAAATGGTATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGTTCAAATTTCCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCTGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGA", "aa": "MIQDEASDLIVEDGTCVGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVEKLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDY", "aa_hexdigest": "820f609c203aacd9f7a1f43746ed372b", "start_type": "TTG", "rbs_motif": null, "genes": [ "mnmG" ], "truncated": "both", "pseudogene": { "start": 705812, "stop": 707581, "observations": { "mutation": [ 706031, 707438 ], "stop": [ 706031, 707438 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8W6", "query_cov": 1.0, "subject_cov": 0.7293729372937293, "identity": 0.991, "score": 865.0, "evalue": 3.45e-314, "gene_start": 1, "gene_end": 442, "reference_start": 118, "reference_end": 559, "reference_sequence": "MTNNFDAIVIGGGHAGIEATYALAKRNFKVALVTLNINRLAMLPCNPSIGGSAKGIITREIDALGGVQGFFADNAMIQIKMLNTSKGPAVWSLRAQIDKEKYSEIILKDIEKQKNITLIQDEASDLIVENGTCIGIETLEHGKLYSKVVIMTTGVYMNSRILRGENIKYDGPDGEKTSSTLSKNLKKYGFEIIRLKTGTPCRIYTDSIDFSKVEKEVLEKNELCFSSRSNKKLDEQTCCYLTHTTLKTKEIILKNLKRSSLYSGIIIGIGPRYCPSVEDKIVRFQEKDTHHIFFEPETARGDIMYINGLSTSMPEDVQDEMIASIPGLEHARVQKYGYAIEYDAINPLNLYKSLESKILKNFFSAGQPNGTSGYEEAAAQGLIAGINAANKLDNLEPLIIKRNDGYIGVLIDDIVTKGTNEPYRMLTSRAEFRLLLRNDNVDERLYKYAYDNKMISKEEHDRIEEKYKIIAETVERLKNEYVSSKSDVGQKYNVTNGMSYLKLLSNPEVDPVDILGSNFPYIDEITIQVRLYGYLKKQESAADKMLRLEQLKLPKDIDYWSVDNLATEARQKLDKIRPETIGQAARISGINPADIQMLIFYLNNKR" }, "paralog": false, "identity": 0.9915110356536503, "score": 1149.0, "evalue": 0.0, "description": "Internal stop codon at 706031, 707438. Nonsense mutation around 706031, 707438.", "psc": { "uniref90_id": "UniRef90_D1J8W6", "gene": "mnmG", "product": "tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG", "uniref50_id": "UniRef50_Q98QV8", "cog_id": "COG0445", "cog_category": "J", "kegg_orthology_id": "K03495", "go_ids": [ "GO:0002098", "GO:0008033" ], "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8W6", "UniRef:UniRef50_Q98QV8", "COG:COG0445", "COG:J", "KEGG:K03495", "GO:0002098", "GO:0008033" ] }, "pscc": { "uniref50_id": "UniRef50_Q98QV8", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV8" ], "product": "tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG" } }, "id": "GLJLFKCOHC_1215", "locus": "GCA013368035_01216" }, { "type": "cds", "sequence": "contig_1", "start": 707584, "stop": 708015, "strand": "+", "frame": 1, "gene": "rlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "db_xrefs": [ "COG:COG1576", "COG:J", "EC:2.1.1.177", "KEGG:K00783", "RefSeq:WP_175949994.1", "SO:0001217", "UniParc:UPI00158E6BE5", "UniRef:UniRef100_UPI00158E6BE5", "UniRef:UniRef50_Q98QV7", "UniRef:UniRef90_D1J8W7" ], "nt": "ATGAAATTAAATATTGTAGCAGTAGGTTCTCTAAGCAAAGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTGATTGAAATAAAAGAACAAGTTAATAAAAATATTGATTTAAAAATAGCCGAAGAAACAAAATTAATCCTAGAAAAAATTCCAAAGAACTCTAAAGTTTATTATTTGTCATTAAGAGGCAAGAAACTTGATAGCAATGAATTTTCGGATTTATTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTAACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAA", "aa": "MKLNIVAVGSLSKEYKTIYDQYLKKISFFCSINLIEIKEQVNKNIDLKIAEETKLILEKIPKNSKVYYLSLRGKKLDSNEFSDLFIEDNITFVIGGSNGVDETYFKNKICFSDLTFPHQLFRIMLIEQIYRGFSIKNNIKYHK", "aa_hexdigest": "9d6819ae8726bd14b86d072b12e81cfb", "start_type": "ATG", "rbs_motif": null, "ups": { "uniparc_id": "UPI00158E6BE5", "ncbi_nrp_id": "WP_175949994.1", "uniref100_id": "UniRef100_UPI00158E6BE5", "db_xrefs": [ "SO:0001217", "UniParc:UPI00158E6BE5", "RefSeq:WP_175949994.1", "UniRef:UniRef100_UPI00158E6BE5" ] }, "ips": { "uniref100_id": "UniRef100_UPI00158E6BE5", "uniref90_id": "UniRef90_D1J8W7" }, "psc": { "uniref90_id": "UniRef90_D1J8W7", "gene": "RlmH", "product": "Ribosomal RNA large subunit methyltransferase H", "ec_ids": [ "2.1.1.177" ], "uniref50_id": "UniRef50_Q98QV7", "cog_id": "COG1576", "cog_category": "J", "kegg_orthology_id": "K00783" }, "pscc": { "uniref50_id": "UniRef50_Q98QV7", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_Q98QV7" ], "product": "Ribosomal RNA large subunit methyltransferase H" }, "genes": [ "rlmH" ], "id": "GLJLFKCOHC_1216", "locus": "GCA013368035_01217" }, { "type": "cds", "sequence": "contig_1", "start": 708470, "stop": 708589, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTATGACTATACTTGGGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATATATCGCAATGATGGCAAAATGA", "aa": "MLRIDHHPVDPDINYDYTWEDATYAAAAEQVGYIAMMAK", "aa_hexdigest": "a8aae5d778d2622d7d44e34280a6413b", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4486.9666, "isoelectric_point": 4.3189905166625975 }, "id": "GLJLFKCOHC_1217", "locus": "GCA013368035_01218" }, { "type": "cds", "sequence": "contig_1", "start": 709116, "stop": 709505, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF01368.27" ], "nt": "ATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAGAAAATTGAAGAAAGAATTAAGGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATTGGTGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTGCCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCTAACTTTAAAGAACGTTTAGAATGTAGAGAATATTTAGACAATAATTGTTTTGATGAAGTAATAAGAATCGATCACCATCCAAATGATGATGATCTAGATGCTTCATTAAGATGA", "aa": "MNKISAEKLAIFKKIEERIKAHKNIVIYHHIRPDGDCLGSQFGMKNLIKVNFPDKTVYTIGDSKGIYSFLEFKMDKLPLEKLDDSLAIIVDANFKERLECREYLDNNCFDEVIRIDHHPNDDDLDASLR", "aa_hexdigest": "0c2a38fe4e5df54da82c9499dd708430", "start_type": "ATG", "rbs_motif": "GGAG/GAGG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF01368.27", "name": "DHH", "length": 100, "aa_cov": 0.751937984496124, "hmm_cov": 0.76, "evalue": 1.3816121060291775e-12, "score": 43.433292388916016, "start": 24, "stop": 120 } ], "seq_stats": { "molecular_weight": 15013.060199999985, "isoelectric_point": 5.709670066833497 }, "id": "GLJLFKCOHC_1218", "locus": "GCA013368035_01219" }, { "type": "cds", "sequence": "contig_1", "start": 709773, "stop": 709901, "strand": "+", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTTGGCTCAATTGATAACACAAAGGCATGA", "aa": "MKIANDVVSFYFPLSEQIKLGIKDPLKANRPFVLGSIDNTKA", "aa_hexdigest": "dbc4e5e42ae0b72b5140856c776f461e", "start_type": "ATG", "rbs_motif": null, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 4679.4378000000015, "isoelectric_point": 9.30261058807373 }, "id": "GLJLFKCOHC_1219", "locus": "GCA013368035_01220" }, { "type": "cds", "sequence": "contig_1", "start": 710342, "stop": 712717, "strand": "+", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF6", "UniRef:UniRef90_A0A454CAF6" ], "nt": "ATGAAAACAAAATTAAAGGTTTTGCTTTCTGCTTCACTAATTACGGCAGTTGCAACAACGGCAGCAATATTTGCTACTGCTATAAAAAGTAAATTAAGAAAACAACACGATTTAAAGGAAAAGTATAACAATTTAAAATTGGAGTTAGAAAACTTAATTAAATTAGCAAAAATAGGCAATATTGAATCTTTGAATGAAATAAAAGCATTGAATAATTCAAAAATTGACAATAATTCAACAGTTAAAGAAATTGAAAATGCTACAAAGGAAATTGAAAAAGCAGTTGAATCAATTAATAAAAAAATTATTAATAAAAAGCATGATGATCAGATTCAACAAGAAATGGCAAATAAAGCTAAGCAAGAATTTATAGCCAAAAAGAATCAATTAAAAAAACTAATTAATTCTTCCCAATTAAGTAATATTGACAAGTTAAACGAATTAAAAAAGTTGAATGATATTAATATTAATGATAAAGATTTGGATGAAGAAATAAAAAATAAAACAAAAGCAATTGAAAAAAATATTGATTCATTGACTAAAAAAATAAATGAAAAGAAAGCCATAGAACAAAAAGCACAACAACTTCAAGAATTTGAAACCACTAAAAAGGCACTAGAAGAATTATTAAATTCTGAAGATGGCCAAAAAGTAAATAGTGATGATATAAAGAAAATATTGCAAAATAATAATGTAAACAATTCATCAACAAATACTCAAATTATTCAAAAAGGCAACGAAATCAAAAAAGCCAAACAAGATTTACAAAAATTAATCGATGATGCTAAAAATAAGGCAAAAAATGGTTTCGATGCTAAGAAGCAGGAATTGAAAAAGCTTTTGGATTCAACAACCGGTGAAATCAATAAGCAAACTGAAGATGAAGAGTATAAAAATATTGAAGTTAAAGGCAATGCTTCAATTAAAGATATTGAAAATAAAACAAAAGCAATTGAAACGGCAATTAATTCATTGACTAAAAAAATACAAAAAAGTCAATGTCTACAAAATTTTAATAATGCAAAAAGAATTTTAGAAGAATTTATTAAAAATGATGAAGATGCTAAAAATGATGAACTAATTTCAAATTTGAACGATGCTAAACAAGAATTAGCCAAATTTAATGTCATAAATGATTCACATGAAATCAATGAAATTAAAGAAGCTACTAATAGTTTAACAAATGCAAAAGCAAAATTGGAACAAGAAATTAAAAACAAAAAGCAAGAGTTGTTTAATGCGTTTGAATTATCTAAAACAGCATTGCAAAATTACATTACAAACGATATTAAAGATTCAAAATATAAAATTATTCAAGATAAATTTAATAATGTTATTGAAGAATATAAAAACATTACATCAAACGACAAAATTTCAAAAATTAAAGACGCTACAAACAATTTAAATGCAAAATTTATAGAAATTGGAAGCAACAAGGCAATATTAGATAAGTTTTATGAATTGAAGCCAAAACTTGAAGATTTATTAAACACCGCAAATCTTAAAGATGATGCAAAATCAGAAAATATTGATTTATCTTCTGAAGATCAAGCTTCTGCTGATGCCAATGCTATAAATAATAGTTCAACTATTGAAAATATTGAACAAGTTAACCAAAAATTACAAACAGCAATAGACAAATTAGCTAAAGATATTCAAAAAATAAATGACGATAAAAAAGCTAAATTTGAAGAATTTAAAACAGCAAAACAACATCTAGAAAACATAATCAAAACCAAAGGTTTAGATAAATTTATTAATATTGATGATGAAATAAAATCAATAAATCAAAAAATTAACGAAAAAATTAATGAATATAGTTCTATCAATCAAGATTCGAGAATTTTTGAAATAGATGATTCAAATAAAAAAATAGTAGCCTCTCATGAAGAATTTTCAAAACTATTGGATGATTATTTATCTAAGAGAAATGAATTAATTAGGAATCATAATACTGAGAAACAAGAATTACAAACATTAATTGATTCTGCAAAGGCAAAAAATGTTGATATTTCTGAGGAAATGAACATTGTAAGTAATGCAAATATTGGTGATGATGACCCTATTGATAACATTAGAAATAAAATAAATAAAATCAAAGATGCAATAAGCCAAATAAATTCAAAGATTAGAGCAAAAAAAGAACAAGCCAAAATAGAATATCAAAAATTCTTATCAATAAAGGAACGAGCAGAAAGCGTTATTGAGGAAATAAAGAAAATTCCTGATTATAAGCCTGAATATAACGAAAGAAAAACAGCAACAATAAATGAACTAAATCAATATTTATCTTCTTATAAAGGACTTTCTGAAAATTCAAATATAGAAGAAATATCTAAAGGAACACTTGATATTTCTAAGTGTTTAACATCATGA", "aa": "MKTKLKVLLSASLITAVATTAAIFATAIKSKLRKQHDLKEKYNNLKLELENLIKLAKIGNIESLNEIKALNNSKIDNNSTVKEIENATKEIEKAVESINKKIINKKHDDQIQQEMANKAKQEFIAKKNQLKKLINSSQLSNIDKLNELKKLNDININDKDLDEEIKNKTKAIEKNIDSLTKKINEKKAIEQKAQQLQEFETTKKALEELLNSEDGQKVNSDDIKKILQNNNVNNSSTNTQIIQKGNEIKKAKQDLQKLIDDAKNKAKNGFDAKKQELKKLLDSTTGEINKQTEDEEYKNIEVKGNASIKDIENKTKAIETAINSLTKKIQKSQCLQNFNNAKRILEEFIKNDEDAKNDELISNLNDAKQELAKFNVINDSHEINEIKEATNSLTNAKAKLEQEIKNKKQELFNAFELSKTALQNYITNDIKDSKYKIIQDKFNNVIEEYKNITSNDKISKIKDATNNLNAKFIEIGSNKAILDKFYELKPKLEDLLNTANLKDDAKSENIDLSSEDQASADANAINNSSTIENIEQVNQKLQTAIDKLAKDIQKINDDKKAKFEEFKTAKQHLENIIKTKGLDKFINIDDEIKSINQKINEKINEYSSINQDSRIFEIDDSNKKIVASHEEFSKLLDDYLSKRNELIRNHNTEKQELQTLIDSAKAKNVDISEEMNIVSNANIGDDDPIDNIRNKINKIKDAISQINSKIRAKKEQAKIEYQKFLSIKERAESVIEEIKKIPDYKPEYNERKTATINELNQYLSSYKGLSENSNIEEISKGTLDISKCLTS", "aa_hexdigest": "5ab3b86327e0d45ae66542d9327c906b", "start_type": "ATG", "rbs_motif": "AGGA", "psc": { "uniref90_id": "UniRef90_A0A454CAF6", "query_cov": 1.0075853350189634, "subject_cov": 0.805050505050505, "identity": 0.955, "score": 1230.0, "evalue": 0.0, "valid": true, "uniref50_id": "UniRef50_A0A454CAF6" }, "pscc": { "uniref50_id": "UniRef50_A0A454CAF6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A454CAF6" ] }, "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 90278.88160000059, "isoelectric_point": 7.883274650573731 }, "id": "GLJLFKCOHC_1220", "locus": "GCA013368035_01221" }, { "type": "cds", "sequence": "contig_1", "start": 713404, "stop": 713946, "strand": "-", "frame": 2, "gene": null, "product": "Phage abortive infection protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6", "UniRef:UniRef90_D1J8X0" ], "nt": "ATGACATCAAAAGAAAAGAAAATGAAAATAAAAAATGTTATATTGAACATTATTGGTTTTCTAACCGTTGCTCTCTTTCTTATTATTGCTATCTTGCTATTTTTAGCAGCAAATGGCATTATGGGAACTATATCTAAAAAATCAAGTATTGTTTGTTATGTTTTTGGTGCAATCTTCTTAGCTATATTCATTTTAATTGTAATTAAAATGATATTAATATTAAAAAAAGAAAATGTATATATAAAAAATGCAATCGATACAGATAAGCTCTTTGCAAATGCTTCTCTAAGCCCAGAAGAAAATGAAATTCATAAGCAATTTATTGAAAAATTTAAACAATATCAACAAAGTAGAAACATATACTTCGGGTATTTATTTACTAAAGCATTAAGCTCATATAAAAGAGATAATATCGATATTTCAGACCACGAAATAAATTCATTAATTGAAAAAATGATTATTGACTGCCATAATGAATTTGGAATTTTTGATGTCTATTTAGCAATTGATTTAGCAAATTCATTAAATAAAAAGCTTGTTTGA", "aa": "MTSKEKKMKIKNVILNIIGFLTVALFLIIAILLFLAANGIMGTISKKSSIVCYVFGAIFLAIFILIVIKMILILKKENVYIKNAIDTDKLFANASLSPEENEIHKQFIEKFKQYQQSRNIYFGYLFTKALSSYKRDNIDISDHEINSLIEKMIIDCHNEFGIFDVYLAIDLANSLNKKLV", "aa_hexdigest": "3ea961e4c7dff9cf994cc226c8728a81", "start_type": "ATG", "rbs_motif": "AGGAG", "psc": { "uniref90_id": "UniRef90_D1J8X0", "query_cov": 1.0, "subject_cov": 0.8450704225352113, "identity": 0.9940000000000001, "score": 331.0, "evalue": 6.8e-114, "valid": true, "uniref50_id": "UniRef50_A0A501X7R6" }, "pscc": { "uniref50_id": "UniRef50_A0A501X7R6", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_A0A501X7R6" ], "product": "Phage abortive infection protein" }, "genes": [], "id": "GLJLFKCOHC_1221", "locus": "GCA013368035_01222" }, { "type": "cds", "sequence": "contig_1", "start": 714055, "stop": 714585, "strand": "-", "frame": 2, "gene": "pncB", "product": "nicotinate phosphoribosyltransferase", "db_xrefs": [ "COG:COG1488", "COG:H", "EC:6.3.4.21", "SO:0001217", "UniRef:UniRef50_D5E5M9", "UniRef:UniRef90_D1J8X1" ], "nt": "ATGGCAGATAGGGCAGATCATTATATAAATCAACCAAACGACTCATATGCAATAAGAATAGGCGGCATAGAAAATCACTCGACTTTTCTAAGCTCAAATTTTGATCCAAAGCATACTTTTGGAAGCATGCCTCATGCATTAATTCAAATGTTTGACGGTGATATTGTAGCTGCTTGCAAAGCATATAAAAATGTTTTTCCAAATGATGATCTAGTTGCCTTAGTTGATTTTCACAACGATGTTATAAGCGATTCATTAAAATGTTTAAAGGCTTTTGGCAAAGACTTAAAAAGTGTAAGAATAGACACATCAAAAGCGCTTATTGATAAAATGTTCAAGGAGGGCGAAGCAGAATTTGGCGTTACACCAAATCAAGTAAAAAGACTAAGAGAAGCATTAGACAATAATAACGGCAAGCATGTAAAAATAACTGTTTCAAGCGGATTTGACGCTCAAAGAATTGCTGAATTTGAAGCCGAAGAGACTCCTGTTGACACATACGGCATCGGTGCAGCACTGCTAAAAATTTGA", "aa": "MADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKI", "aa_hexdigest": "75d1c7c3d0104449e47eb3857299e092", "start_type": "ATG", "rbs_motif": null, "genes": [ "pncB" ], "truncated": "both", "pseudogene": { "start": 713947, "stop": 714885, "observations": { "mutation": [ 714057, 714624, 714684 ], "stop": [ 714057, 714624, 714684 ], "directions": [ "3-prime", "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X1", "query_cov": 1.0, "subject_cov": 0.5238095238095238, "identity": 1.0, "score": 355.0, "evalue": 1.03e-121, "gene_start": 1, "gene_end": 176, "reference_start": 125, "reference_end": 300, "reference_sequence": "MKKINLKDYISIYFYKTQKISKKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGIWEGIIDGILARQSSIATNAYWAIKAANGKKIVSMADRADHYINQPNDSYAIRIGGIENHSTFLSSNFDPKHTFGSMPHALIQMFDGDIVAACKAYKNVFPNDDLVALVDFHNDVISDSLKCLKAFGKDLKSVRIDTSKALIDKMFKEGEAEFGVTPNQVKRLREALDNNNGKHVKITVSSGFDAQRIAEFEAEETPVDTYGIGAALLKIWVNFSADATRLNGHNIAKAGRGYLKNPQLITYKEAK" }, "paralog": false, "identity": 0.9903846153846154, "score": 613.0, "evalue": 8.42e-226, "description": "Internal stop codon at 714057, 714624, 714684. Nonsense mutation around 714057, 714624, 714684.", "psc": { "uniref90_id": "UniRef90_D1J8X1", "gene": "PncB", "product": "nicotinate phosphoribosyltransferase", "ec_ids": [ "6.3.4.21" ], "uniref50_id": "UniRef50_D5E5M9", "cog_id": "COG1488", "cog_category": "H", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X1", "EC:6.3.4.21", "UniRef:UniRef50_D5E5M9", "COG:COG1488", "COG:H" ] }, "pscc": { "uniref50_id": "UniRef50_D5E5M9", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D5E5M9" ], "product": "nicotinate phosphoribosyltransferase" } }, "id": "GLJLFKCOHC_1222", "locus": "GCA013368035_01223" }, { "type": "cds", "sequence": "contig_1", "start": 714682, "stop": 714957, "strand": "-", "frame": 2, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "PFAM:PF02749.22" ], "nt": "ATGAAAAAAATCAATTTGAAAGATTACATTTCAATTTATTTTTACAAAACTCAAAAAATATCAGAAAAGTTCAAAAAAGATCAAAACATTACTCTACAATTCTTTCAAAGACATAATGATGTCAAATTATGTGGAATAAATGAAGCAATTGATATTTTAAAACAAAACACTGATATTTCTAAATACACCATAAGATACATGCCAGAAGGCAGTATAATCCACAATAGAGAAGTAGTTTTAGAACTTGAAGGCCCTTATTATGAATTTGGTATATGA", "aa": "MKKINLKDYISIYFYKTQKISEKFKKDQNITLQFFQRHNDVKLCGINEAIDILKQNTDISKYTIRYMPEGSIIHNREVVLELEGPYYEFGI", "aa_hexdigest": "25c5857975b38a372459a90621109c0e", "start_type": "ATG", "rbs_motif": "AGGAG", "hypothetical": true, "genes": [], "pfams": [ { "id": "PF02749.22", "name": "QRPTase_N", "length": 79, "aa_cov": 0.8681318681318682, "hmm_cov": 0.8481012658227848, "evalue": 3.6096365643266696e-11, "score": 38.595455169677734, "start": 12, "stop": 90 } ], "seq_stats": { "molecular_weight": 10912.484899999996, "isoelectric_point": 8.625950431823732 }, "id": "GLJLFKCOHC_1223", "locus": "GCA013368035_01224" }, { "type": "cds", "sequence": "contig_1", "start": 715282, "stop": 715539, "strand": "+", "frame": 1, "gene": null, "product": "hypothetical protein", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2", "UniRef:UniRef90_D1J8X2" ], "nt": "ATGGTGAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCAATGATAGCTGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCATTAAGAAAATGCTATTTGATTTCGCTAATTCCATTATTGTTTTTTGTAGCATTGGTAGCAAGCTTTGTATCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAACGTTGCTATAGCAGGATAG", "aa": "MVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG", "aa_hexdigest": "581490f79f53323a0d0b60fb7875800c", "start_type": "ATG", "rbs_motif": null, "genes": [], "truncated": "5-prime", "pseudogene": { "start": 715114, "stop": 715539, "observations": { "mutation": [ 715195, 715210, 715252 ], "stop": [ 715195, 715210, 715252 ], "directions": [ "5-prime" ] }, "inference": { "uniref90_id": "UniRef90_D1J8X2", "query_cov": 1.0, "subject_cov": 0.6028368794326241, "identity": 1.0, "score": 155.0, "evalue": 3.73e-47, "gene_start": 1, "gene_end": 85, "reference_start": 57, "reference_end": 141, "reference_sequence": "MAKNQDKKMLSPEEKEIENIKKISKNGWHFSYWSLFFILGVIAILIWAVESESSSSMVKYPLSIIFIILCIAMIAGMYTINVMGYKLAIKLNNESLRKCYLISLIPLLFFVALVASFVSSIKLKEIYIDKKSQFQNVAIAG" }, "paralog": false, "identity": 0.9716312056737588, "score": 256.0, "evalue": 1.42e-89, "description": "Internal stop codon at 715195, 715210, 715252. Nonsense mutation around 715195, 715210, 715252.", "psc": { "uniref90_id": "UniRef90_D1J8X2", "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef90_D1J8X2", "UniRef:UniRef50_D1J8X2" ] }, "pscc": { "uniref50_id": "UniRef50_D1J8X2", "db_xrefs": [ "SO:0001217", "UniRef:UniRef50_D1J8X2" ] } }, "hypothetical": true, "seq_stats": { "molecular_weight": 9565.66899999999, "isoelectric_point": 9.555197715759277 }, "id": "GLJLFKCOHC_1224", "locus": "GCA013368035_01225" }, { "type": "cds", "sequence": "contig_1", "start": 715710, "stop": 715910, "strand": "-", "frame": 3, "gene": null, "product": "hypothetical protein", "db_xrefs": [], "nt": "ATGAAAATTACTTTACCTATAATACTTGTAATTGTTGGTTATATTTTAATTACATACATATTAACAACATCAATACTATTGATGAAAAAGCCACAATTAATAACTAAACGCCACACTTTAATTTCGCAAGTTGCAATAGCGTTTATTCCAACATTAAGTGTTTATGCTATAACTTCATTGGATAATTATTTCATCCTATGA", "aa": "MKITLPIILVIVGYILITYILTTSILLMKKPQLITKRHTLISQVAIAFIPTLSVYAITSLDNYFIL", "aa_hexdigest": "022642b7f95753253b7474b93d30f264", "start_type": "ATG", "rbs_motif": "4Base/6BMM", "hypothetical": true, "genes": [], "seq_stats": { "molecular_weight": 7463.145299999999, "isoelectric_point": 9.777226829528807 }, "id": "GLJLFKCOHC_1225", "locus": "GCA013368035_01226" } ], "sequences": [ { "id": "contig_1", "description": "[organism=Metamycoplasma hominis] [strain=SP3615] [gcode=11] [completeness=complete] [topology=circular]", "nt": "TTAAGCTGCTTTTGCTGTAAGCTTTGGTTCAACTTTTTGTTTAAAGAATTTTGTTCTTTGGAAATAGTGAACAAATATATGTTGCAATATTAATATAAATGCTCCAACAATTCAATAAATTTGTAGTCCGGCGCTAAAGAATGCTCCAAAGATTATAAATACTAATGAAATAATATTTGACATACGATTTTGTTTTTTCATTGCTGCTTTTTGATAAGCATCAACACGTAATGACTTCTTTTTCTTTCAATTTAAAATCTTTGGCATATATTGCGATAGTGCTTGCATACCGACAGATATTAAAATTATTGGTAGATATATGAATTCTTTGTTAGAAAATACCCGGTTTAGTGAGTTTGCTGATAATTGAATTGAATATCAGGTTGCTTGTTTTATTTCTGGAGAGGTATTTATAACTCTAAATACAACAATTAATATTGGCAATGCAATAAACTGAGGCCAAATCTGTGATCAAGGAGAAATTTTTTCTTTCTTATATAATTCACTAATTTCAGCTTGCTTACGTTGCATCATTTGTCTGTCGCCTTTGTAAGCAGCATATTTGGATTCAATTTTGGCTTTCTTTTGGTTGAATTCTTCCATTTTTGCTTGACTAAACAAAGACTTGAATGAAACACCAAATACTACTAAACGAACAATTATAATAACAACTATTAATGCTAATATAATACTTCATCCTGTTGTTCCAAGTCCTTTTATAATTGAGTCCATAAATAAGTATATAGGTTGAACAAATATACCATAAAATGGTCCTTGTGCTCAATATTCTTTGTATGTTGAAATAGGTTTTTGACTCAATATTGCGCTATTATTAAATATTGTGTTTGTTCAATATTCTGAATTGTAATTTGAAGCAGGACTTAGACTTGATTTTCTATATACAAAACTACCATCATTTGTTTTAGAAGGCAATTTTACATAATTTACATTTGATAAGTATTGTTGGAAAACACTTTTAACAAAAGTAAATAGTTGAAGTAACTTTCTTCCTTCATCTTCGTTGGCTAAAAGAGTTTTATTTTTTGCTTTTTGTTCGTAGCTTTTTATAAATTGATTTCATTTTTCAATTATTTGGTTAGCATTTTCGCTACCATTTAATTTTAATGCCTCTTTAAAGATGTTAACCTTATCATCTTTAAAGGTTTCCAAACTTGCTCATTTTTGTAATGTATTTGAAACAAGAATTTGAAAAATATCTCTTAGAACAATTTCATCAACTGATGAACCACCAGTTAACTTTCCTTCTACTATATCATAGTTAAATTCTTTTATTTTTGTTCAATCTGTTGGTATAATGCCATCCTTATCTTTTGGCTTAGTGTCAAACGATAAAGAATTAGGAATTAAAATTTCACTGAATTTGGTTAATGGAGTGTAATTTTTGGTTTTGTAATTTCCCTTTTCATCACCCAAATTAAAGTAGGCATAATTTCCATTTACATTGTAGATGTAGCCACTTTTATCAGATGTTCCTTTTTCTATTATATTTGTTCAATCTTCATCATGAGAAGATAAAACTTTTGCTTTGTCGTTTTTTGGCATTTGCAATTGCAATCCAAATGAACGAGAACCATATGCACCATAATTAGGATCTCTCTTCCTTAACTCATTAATAGCATTAGGTTCTTTTAAAGCAAGATATGTATTGGCGATAATATTTTTATCATTTATTTTGTTTGGTAAGGTAAAGAAATTACCAGTGCTATCGTATCTATAAGTGACAATATTTGGTGAGATGTCACTCTTTTTAGAATATAGCTCTTGTCCAGAACCGACTTTATTACTTGATTTTACAGTAAATGATTGTACACAACCAACTAAACCGATAGCCAAAAATAATATAACTATGACAATTTTTATTCATTTTCAAACTTTTAACCAAATATTTTTCGGTGTTTTGTTTTCTGGTTGACGAACTCGAAAGTCTTTATAGAATTTAGAGACATCCTTATTTGCCATCAGCGATCCTTTCATAAAGTTTTTTTACTTCTTTAACTTTTTGATTCCAGTCAAGACTCAAGAAGCCTTTGCGAGCAATAATCACAGTTTCGTAATTTAAAGTGCTAAAATCAATGTTTCTTAGAATTGATCTGATTTGATTTTTGTAATGATTTCTCAAAACAGCATTTGCAAATTGCTTAGGGATTGAAATACCCACTTTAAAGAAATCGGATTTGTTAAAATAAATTATTAAATTTCTTGAAATCACTTGATTGTGAGAATCAAGAATTTTTTGAAATTCTCAATTTTTTTTAACAACGTTATTTTTATTTATCATAATTTAAAAATATGCAAGGTATTAGACTTATTTATTGTCTGATACAGTTAATGTATATCTACCTTTTGCACGTCTAGCTGCTAAAACTTTTCTACCATTTTTAGTTTGCATTCTAGCCATAAAACCATGAACTTTAATGTGTTTGCGTTTTTTTGGTTGGTATGTTCTTTTCATAACAATTCTCCTTTGCGTTTTTATATATAAAATAACTTAATTTATTTTACCAAAAATACGACAAAAAAAGTATATAAAATACCTAAGAAAAAAAATTTAATATTTTTTATGTTTTTAATGTAAATTAAAAGAAACATAAAATTTAAAGTTAATAACAAAATAAACAAAGAAATTATTTAAATGTTTATTATGTTATTTTAGTATAAAAAACCTCTTATTTTAAAAACAAAAGCCCTAAAAAAATGTTAATAATTTTGTTAATAATTTGTAAATTAGCATTTATAAGGGCTTATTAATTTATTAATTTTGAATTTTAAAATATAATAGAGGTATGAAAACTACAAATCCCTCTGAATTAGATTTAAGAACACATAATATGTCATTTCAAAAAGAATTAGTTAATACTGCAAGTGATCCTTTAATATATGATCAATTCTTGTCGTCATTAGAAATTGTAAAAGAAAATAATAAAATGATTTACATATTAGTTAAAGGACCTGAAGACGTTTTAACATATATTAAGAACAGTCATAATGAAAGTATTAAAAGAGCAATTAAAAACGTTTATGGTGAAGAATATAATTTGGTTTATATTAGAAATTTAGATGAAATTAAAAGCTTACAAGATAGCAATAATGTTGAATCTCCAACTAATTCATTGACTAATAATTCTAAAGAAAATTTTAGCAATATTAAGAAGAATTTAACATTTGATGATTATGCAATAGGAAAATTTAATAATATGGCTTTAAAGGCAGCAAAGGCTATTTGCAATAGTGAAAAGATATTGTTCTCTCCTTTATTTATTCATGCTTCAAGTGGGCTTGGTAAAACACATCTATTACATGCTATCGGAAATGAATTATTAAAACACGGTAGAAGTGCATTATATATAAATCCTGATTCATTGACAAGAAGATTAGTTGAACAATTAAAATCAAAGAATCAAGAACAAATTAATAAAATTATTGATGAATTGATGTCTTATGATTGTCTAATGTTTGATGATGTTCAACAATATGGTAATCGAGATTCTACATTAAATGTCTTATTTAATATTATTGATAATATGATAATGAACGATAAGCAAATAATCTTTTGTGGTGATAAAAAACCGGATGATTTAGGTGGATTTGAACAAAGATTTATAACTAGATTTAATGGCGGATTAACTGTTGAAATTTTAAAACCAGAGTTAAATGATGTAATTAATGTCTTGAAATTTAAATTGCAAGAAAACGGTATAAATCCGGAACTTTGAGAGGAAGAAAGTTTAAAATTTATTGCCAGAAACTTCTCTTCATCAATAAGAAATATTGAGGGTGCAATTAACAGAATAAAATTATTTCAAGATGGTGATGATGGCTTTTTTACATATGATATTAATACAATAAAATCAATATTTAACAATATGACTCAAGTTGCCGAGAATATTACTCCTGATAAAATAATTGATGCTGTTTCTAAATACTATAAAATTGACAGAAAAAAAATATTGAGTAATGTAAGAACAAATGATGTGGTTAATGCTCGCAGAATAGCAACATATTTAGTAAATAAAAATTTTAATTATACATTGCAAGAAATAGGCAAGGTTGTAGGAAATCAATCACATTCAAACGTTATTGTTTCGCTTAATTGAATTGAAAAAAATGCTAATAGTAATCCAACACTTAAATTAGCATTGCAAAAAATAGAAAGCAATCTTAAAAAAATTTCGTAATAGGTAGGTATAGAAATGAAATTAAAAATTGATAAATTATTATTAGATAATGCAATTGAAAGAGTATCAAAATCTGTGGACGCAAATCCGTTCTTGCCAGTCATGAAAGGTATTTTAATTCAAGCAGAAGATAACAAAATAACATTAATTGGTTCAAATGGTGAAACAAGCATAAAACACGAAATTGAAACATCAATTGATGCTCAAATTGAATTGCCAGGAATTGTATTAGTTGAACTTAGCTTGTTTAAAAATATTATTAAGAAGTTAGATGGCGAAATAGTATTAGAAGTTGTAAATAATATTCTTAAAATTAGTACAAATAATGAAAATTATAATTTAAATATATATGATGTAAATGAATATATTGCAATCGATTTTGCATGTTATGGGGAACAAGTAAAAATTAACTGGAACGATTTTAAATCTTTAGTTCGTGATGTTATTTTTGCCGCTTCAACAAACGAAGTTAGTCTAATCTTGTGCTGTGTCAATATATCGGCAGCCAATAAAAAAATAAAGTTTATAACAACAGACAGATTTAGATATGCTCAAGCAGTTCAAGACATTTCTGAAGATGTAACATTCAATATTTCAATATTAGCTAAAAACTTAAGAGATATTACCAACTTTGATTACAATGGCGACGTTATTTTAAATATTTCTGATCAAAAGATTTCATTTAAATTAGACTCAACAACTATTGAGTCAAAAGTTGTTGATCAATTGTATCAAGATGTTTCAAGAATTGTTCCACAAGAATTTGACACCGAACTTAAAATAACTAAAAAAGAACTAAATAACTTGTTAAATAAGGCATCAGTTATTATTAATGAAAACTATAATAAAATTAAGTTGCACATTGTTGATAATGTTTTAATCATTTCATCTGCTAGAGAAGAAATTGCTAATGCTGAAATTAAAACAAATAATTTTTCTTACAACGGTGAAGAATTGAAACTCGCATTGAATAGTAGATTCTTAAAGGATGCTATTTCTGTGTATGAAGATAATTTGAATATTTTATTTACAAAAGATAAATTAAGAATAGTCATAAAATCAGATTCTAACCCAAATGTGCTACAATTGATTACGCCACAAAAAGGATTCTAATGGAAATAGAAATTACTGGAGAATTTATTAAATTAAGTCAATTTTTAAAGAAAATTGATGTGTGTCCAACAGGCGGAATGGCAAAATATTTTGTTCAGGTTCACAGAATATTGATTAACGGGAACCTTGCAAACGGAAGAAATGCTAAGATTTATCCCGGCGATACCGTTTGAATAGATGACAATGTTTATTTAATAAAAGGACAAAAATAAAAATATTTTTTATAAATATTATAAAATATTATAATTATTAAGCTTAACAATAACATAAAGGAGAAATCATGCCAGGAAGAGATCAATTAACAGGTCAAAAAGCTCTAAGTGGAAACAAAAGATCACACGCTTTAAACACCGCAAAAAGAACATTTGACTTAAATCTTCAAAAAGTTACTGTTTTAACTGAAACCGGAAGTAAAAAAACAGTTCGTGTAACTGCAAAAAATGCTAGAACACTAAAAAAATATGGTCTAGTTGCATAGTAGTTTTTTATAAAAATAATATGAAGTTGCTTAGGCAACTTTTTATTTTATTTATGAAAAAAAGAATAATAAAAAAGCGCAACTCTCTTTCTTAGTCCATACTAAAGAGAAATTGCATAATTATTATAACAAGTTATAACAAATTATTAATAATAAGTTTTTTAATTTCATAATAAATGTAATTTCTCTAAATATTAAGGAGAATTTATTATGAGAAATATAGATCAATTAGAAATCTTTTATGCTGGATTTGAAACGAAGGTTCAAACACATAACAGAAGTGCTAAAACACTTAAAAATTATACATTTGTTTTAAGACAATTAAGAGACTGGGACACCCCTCGAAGAATTAATAGAATAAATTAATTTTATTTTTAAAAAATTAAGCAGTTGGCTTAATTTTTATTGTTTATAAATTTGAATAAATTAATAGAAATTTCTTTTGTAATTTTTTCAGCTCTTATATTTAAATCTAGTTCATTGCTTTTTAAAAATTCAGAAATTTTTTGTTTTAAATTGCTTGGAAAATTATTTAATAAAGTTTTACGTTTGTATTGAAACATTTGTTTAATAAATTTCAAAATCTCAACTTGATTGTTTCAAATAAAATCATTATTCTTTATTAGATTCAGTTGAATAACTGCCGAATCCACTTTAGGAGCGGGATTAAATTCTTTTGCCTTAACAGTGAATAAATATTTAGGTTCATAGAGAAGTTGTATTGATGCCGCTAATTTACTATATTGAGAATTGCCCGCTCCACAGCATATTCTATCTGCTACTTCTTTTTGAACCATTAATGTTATTTTATTAATGTTTTTAGCGTTATCTAATAATTTAAAAATTATATCGCTTGTTATATTGTAAGGAATGTTTCCAATTACTTCAAAATCAGTATATTTTAATAAATCTGTTTGTAAGAAATCTTGGTTCAATATTTCAACATTGCTTGAATAATTTTTTTGTAATAAAAATTCGTATAATCTTTTATCAAATTCAATACATACAAGTCTATTTACAATATCTGCCATTTGATCAGTTATTGCGCCCATTCCTGGGCCAATTTCAATAATATTTTTGCCCGCAACGTTTGCAGCCTTTACAATCTCTTTTTGAATGTTTTTATTGCTTAAGAAATTTTGGCCCAATGATTTTAATGCTTTAATCATAGATTTTAAAAACCTTTCTAACATTCTTTTTAATTTGTTCAACTAAAACATCAACATCAATTTGTTTTAAATTAGCAATATATTCATATGTGTATTGAACAAAAGGGCTAATGTTGGGTTTTCCTCTCATTGGAGTTGGGGCTAAATAAGGTGTATCAGTTTCACAAAATATTCTATCTAAAGGAATATTTTTTACTGCTTCTTGTAAAGGCTTAGCATTTTTAAAAGTAACAACCCCGCTTATTGAATAATAGATATTTGAATATGTCAAGCATTTATTTAAAAAACTAAGATCCCCACTAAATGAGTGAAATATAAAATTAATGTCTTTATATTGAGGCAAGGTTATTATATCTAAAGAATCATCTAAAGCATCTCTAATGTGCATAATTACTGGTAAATTATGTTCTTTTGCAAAATCTATTTGGGCAATAAATCCTTGTTTTTGAATTTCTTTGCTAGGCGAATCATCATAATGATAATCAAGGCCAACTTCTCCAATTGCAACAACATTTGGTTCATATATCTTGTTTAAAATTTCTCTATCTTGCAAACCATTTGATAATGTTGGATGTATTCCAATAACAGGATAAACAAAATCAACCTTTTTAGCAAGTTCTATTGCTTGTATCGAATCATCTTCGTTTGTTCCACATACAAAAAGCTTTTCCATATTTAGGTTATGCCATTCATTAACTATTTCAAGAGGATCTGAATAGTATTCGGTTGATGGGTGGGTGTGTAAATCAATAAATTTCATTATAATTCTCCTAATATATCTTTAAAAATATCAATTATTTTTAATTCTCAAATTGCTTTTGATTGCCTGTATTCTAGGTTATTATTTGATTCCAAACAATCTGAAATCACCTTGAATGAATAAAATGGTTTGCCAAAAATAAATGAAGTATGTGCCAATGAACAACATTCCATATCGCCTAATAAATTATTTGTATTTCAATTTAATATATTTGAATTTATTATGCTTTTGTTAATAAAACTATCACCGGTTATAATATTGGCTTTTTTAAAATTATTGCAAATTTTTAGATCTTGATTGCAATAAAATACATCTTGCAGATTAGGCAATTGCCCGATTTTATAATTAGGTAAGGCAGTTAAATTAACGTCATAATATTTGCAATTTTGTATGCAATAAATATCTCCAATACAACCATTAAAAATTGATATAGCAGGTCCAATGTTAATTACTTTTAATATATTGAATTTAGTCAATAAATAACTTAACATTAAAGCAGAATTAGTTTTTCCAACTCCACAGTGCGCTAACAAAATTTGTTTATTGTTTCAATTTAATTCATAAAGATTTTGAAAATTGTTTTCAAAATCAATTTTTATTTGTTTTAAATGCTTGTTATATTTTAGTCTTAATTGTTCAGTTTCTAGTGGTTCTGCAAAAATAAATAAATAAAATTTCTTTTCCATATATTTTTATTATTATATTAATAAATTAATAAAAAATACATATTTAAAATAAATTAGTTATTTAAGTCGTTTTTGATTAAAATTAACAAATTAACTCAATATCGTTTTAAAATTAAAATAGTATGTGAATATTAGAAATAGTTATTTCTGTGTTAATATGAATTGTTATTATTGTAGGAATTGCTTTTGCAATTCATAAAAAGTCATCTCGACACAATTATGATAAAACAATAAATTTAATCAAAAGAGATTCATCAAAACAACAAAAAGGCAACGATGGAGAATTTTTAGTAGCTCGAAAATTAACATCTTTGAATGAAGGCAAATATAAGGTTTTAAACGACATTCTATTGCAAATTGATAATGATAAAACAGCTCAAATAGATCATATTGTTGTTTCAACTTACGGTATTTTTGTAATTGAAACAAAGAATTGAAATGGATTTATTACAGGCGGTAGGGATTCATACTTTTGATCTAAAAGAAATTCGGGAAATAATATTGAAATAATGAAGAACCCAATAAAACAAAATGAACGACATATTGAAGCATTAAAGCAGATATTTTCAAAATACAATATAAATATAAATAAAGAATTATTTTATTCTCTTATTGTATTTGTCCAAGGCCAAAAGCCCAAAATTGATGATCTGAATAATTGCTCTGTAATACCTCTCAATGATTTAGTAAAAAAAATTCATTCATACAATAAAGAAATATTAGAAGAATCTCAAATTAAAGAACTTATAGAATTGATTCAACAAGAAAATGTTGAAGATATACAAACAAGGTTAGAACACAACGAACGTGCAAAAAAAGTTTTACAAGTTCAACAAGATAAAATTAAAGAACATATTTGTCCACTATGCAATGGCGAACTTACATATAAAAATGGTTTACACGGCAAGTTTTATGGTTGCTCTAATTATCCAAAATGTCATTTTTCAATAGAAAATATTGATTAAATTAATGACTTTTATAAGTAGCCTAATTATTTTTTAAATAAAAAATTACAAAAAAATATTTTTTTAACAATTTATTTAAAAATGGCTTATAATAATTACGCTATTAAATAAGAGCCACTGCCCAGGTGGCGGAATGGTAGACGCTACGGACTTAAAATCCGTTGGCTGTAATGGCCGTGCTGGTTCAAGTCCAGTCCTGGGCACCATAAATGCGCCCTTAGCTCAGCAGGTAGAGCAAATGGCTTTTAACCATTGGGTCAGAGGTTCGAATCCTCTAGGGCGTACCATTTCAAGAAATTGATCAAGAGAGCATTGCTCTCTTTTTTATTATTTTTATTTATAAAAAATCAAGTTTATTTTTAAACCTGATTTTTGAATTATTCGACTATTGATTCTAAATATTGAAGTTTATTGTTCCGACTATTGAATTTTATTTTAGCTCCATAAGCAATAATAGTTTTTGGATAACAATGTCCGAAATTAACATTGTAAATTATTGGAATATCAAAAGGAACAACTTCTTTTCAAACTTCTTTATATTCATTATAATAAACACGATTTTGAGGGCATCCGATTAATATTCCTTGAATATTTTTAAATAAGCCAACTTCTTTGAATTTTAGCAACATCTTTTTAAGTAATTGTGGGCTTGGCTTTTCTTCACTTGTTTCAAGAAATAAAACCTTATCACATAGTTCATTAGGTTTCAATATTAAATTATATTTTTTAAATATTTCAGTTTCTTCTTTGTTTTTTTCTTCATCTAATCCACTATAAATAGTTTCGATGCAACCACCTCATAAATATCCCTCAAAAGATTTCTTTTCATTTAAAAATTCATATTTTGTAATGTTTCTATGTTTCTCTCTTGGTATATTCAATGCTTTTTTTGAAAATTCATTTCGTTCTTCATATCAATATGCACTTGGTTTAATTTCAAATTTAGTTTTATTATCGAATAATCATTTAAATGAGTTTTTAGTATAAGGCAACATTTCTTTGTCAAGTTCAGCAATATCGCATAAAATGTTGTGCCCATAAAATGTTTTTAGTCCTAATCTATAAAACATTAAATGGTTAATGGTTGAATCTGAATATCCAATAAAAATTTTAGGATTATTTAAGACTAAATTTTTAAAGTTTTCATCTTCTAATAAAAGTGGAATAGTTTTGTACGTGTCAAATCCACCAATTGCACAAATTATTGCTTTTATATTGACATTGCTAAAAACTTCATTTAAATCCTCAACTTTTAACATAGGATTTTCTTTTAAAACTTTTATTCCTTTAAGTGCGTTTTTAGCAATCATAACGTTAAGTTGAAAATCCAAATTGAGTCTTTTTATTGCTAAATCTAATTGGTGACTAGCAAATTCTTCTCCAATAGTTCCTGCAGATAAGCTTACTATTGCAATGCCATTTTCTTTACTTAATTTTATTGGTTTGATCATATAGAAATTATATTGAAAAGTTTATTAAATTAAAACAAAAAAATTAGACAAGCAACTTTGCACAAGTCTAATTTTTAGGGCTAGATTTAATTATTTATTGTTTTGAAAATATTTGAATAATGATTCAATATCTGAAATAAATTTATGTTTATTGATAATTAATTCTTTTATGCCGTCAGGAATAGATACATTGACCTCTTTATCAAGTTCTGCATTATCACTTGCCCCTAAAAAATAAAAGTCTTGGTTTTCTTCATCAACATAACAAATTAAAAACGTGTAATTATTATTTTTTTCTACGTCATCAACAAATAAAGCATTATCAGGTTTTTTATTGTCTAAATATTTTTTATAGCTAGTAACTATGTTTTTAGTTTTTTGTTGATTATAATCGTGTTTTGCACTTTTAACTTCAATATATAAATAATTTGTTACTTCACCACTATTTATTTTAAAAACAAAATCAGGATATGAAGACTTAATTTCTCTTTCTTCATCAAAATATTCTAATTTTAAACCATGAAAAACAGGGTTTTTTGTATAAACTTTGACGTTTATTTCATTTTTATATTCACTAATTATTTTCTTTGCTTCGTTAATGAATAATGTTTCGGGATTTGAATCAAAATGATGAAGTCTATCGTTTAAATCTTTATATGCATAATTTTCTGTTTTGTTTAGTTTAACATTATTTTTTTCTATTTGATCAGGATCAATAAATACTTCATTAGTTTCAGGTAATGTTTTATTGGCTAATATTTTATAAAGTTGATTTGTTGAATCTATTTTTTCAATATATTGAGCATATTGTAATTTAAATGTATTGAAATATTCTTTTATTAAGATATATCAAAAAATATTTCTTGTTATTGGACTATTGTTTAAATAAATAGAATCATATTTTTCATTTAACGCTTCAATAAGAACTTCGTTGAATATTTTTTTATGCATAAGAAGAAATTTGTTGATAAATAATTCTAATTCAATTGAATTATTTATTCATGAATAAACTCTAATTTTATTTTCAATGTTTCCTTGTTCTTCTATTAATTTCTTGTTGTTTTTAATATATTCTTTATGGTATTTTTTAAAATATTCATCAATTTTTTCTTTAACAATAGAATTTTTAGCTTCTCTAATAAAATCTGCTCCTATTATTGCTTGTTTGATTTTTACTTTATTTATTTGGCCATAAACAAATTTTTCATTTTCAAATTCTCGTTTTAAAATCAATGTTCTTCTTATATTTTCTTTGGTTTGAGCATTTGAATAAATATAGTATCTATTGCCTTCTCTAGAAATTTGATCTTTGTTATAACTTGGATTTGGGAATCTTTTAATTCTTCCTATTGTTTGAATAGACAAACTACTTGATGACACTTTTCTAAGTTGCACAAGCATGCAAGCCCTTGGAATATTTCAACCAGTTGAAGGACCAATTTTAAAAATTATTGCATCAACATTAGAGTCATTTTTTGAAATTTCTTTTAAGTTTATTTTGTGATTTTCTTCCTTTATTAAAGAAGAAGATTCCACTTTATCATTTGAAAAGTATTTAACATAACTCAAATTACATTCATTTAATTTTTTAATTATGTTTTCAATGCTTTCTTTAAAATCATCTTTTTCTTTGGCAGTTGATTTTTCACTTGGTTCATTTTCAACTTGAATAAGCATTGCTGGTCTTATATTTTTTAAATCACTATTATTGTTTGCATATTGGCTTTGAACTTCTTTAAATGTTTCACATGCTTGATTTAGTAATTCATTATCATCAATTAAATCTTGACTTGAGTCAGTTATACCCTTGTTATAAATTAATTCATCTTTTAGCAATTTAATATCATCTTCGGCTAAATCCTTTTCATCAATATAGACAAGTTTATGTGTTCCTTTAGGAGTTGCTGTCATTTTTATTATTAAATTGGCTGCTTTTTGAATTTTATATTCAAATCTAACTTCTTCTTTTTTTAATTTAGATTCATCATGGATTGTATCAATATCTTCAAATTTGAAATTACTTCTACCTTTTATATTTCCACCATGATGCGCTTCATCTCTAATATAAACTAATGTATAGTTTTGATATTTAATTTGATCAAGAAAGCTTTCAAGCAAACCTTGTTCGGTAAATATTCTATTTTTACCAAACGATGAAACACCGAAGATTATTACTTTATTTTTTTGAGGAATTAAATTATATGTTGAATCTTTATTTTTTTGAGAATTTGAGGAAGGAGATTCCTTTCTTTCTACTTCATATAAACCATTAATATATCTTTTATATTCATTTAGGTTTGATTCCATTTGATAAGGTAAATCTGCCGATGAAAGTGTGGCAATAACAAAGACTAACTTGTTTGGTTCACCATTGGCATTATTTTCTTTTATTATTTCATTTATAGTATTAGCTAACATAAATGTTTTCCCAGAACCAGTTGGAGCTTGAAAATCAACTATTTTAGGTTCACTAGATTGAATTGCAATTTTATATTCTTCAAGCAATTGTTTAACTGCTTTTTCTTGTACATTAGTTAGATTCATTGTTATCTTTTCCTTCAATTGGTTTTAAAGCAGTTAAATTTAGTAATATTTCTTGTTCGTTAATTTTATTTACATTCGATGAGTCAAAATCATTAAGCATTTTTACAAAATCTTTTTTAATTATTTCATTGTTGTTGGCAAGTACATCAGTGTCGTAATATTTGATTTCAAAGACATCAAGGTTTTGATTATATGGTTCATTTTTCTTTATTCAATCAAAGTCGGTTTCACCTTTACTTCCCTTGCCTTTATTAATTCTATAAAGCCTTTCATAGCAAACATTTATGCCAATATTATTTTCATTATTTGTAACTAATGTGAAGGTTCTATTACCCCCATCTTCTTTATTTAAATCTAAAACTGCATGACCTGTTGTTCCTGAACCTGCAAAAAAGTCAAGAATACGAGCATTAGGATTAGGACAAGAAGATATTATTCGTTTTATTAAATTTTTGGGTTTTGGATATGAAAATTTATCGCAATTAAAAATTTGTTTCAAATCTAATGTTCCATAATAATTTGAATAGATATTATCCATTAATTTTGAAGAAGACCAATCAATTCCTCCATCTTTTTTACATAATTTGTATTCTTTTCCTTCTTTTTTAATTTCTAGGTTTTTATAATTTTTTACATACAAAATACCCTTTGAATTTTCAACAATTATTTCATTTTTAATCATTCAATCAATTCTTTCTTTGCTAAAAGATCAATTGCCACCATTTCTAGGTTTAAATATTTTATTATTTAGTTCAACTTCAAAATTGTATTTGATATTGCCGGCGCCACCATCTAATGGGTGTTTTAAGGTATAGGGGCCTTTTGCATCTTCAAACTTATAATCGTCTCAATCACTTTCTATTTTTTTAAATTTAAAGGCTTTATAAATATTTTTGCAATAAACTAATATATAATCTGTATCTTTTTGAATATTTGAATTTCCAAATCTTCCGCCACCTTTTTTGGTTATTCTTGCTAAACATGCCACAAAATTTTCTTCTCCAAAAATTTCGTCCATTAATACTTTTAAATATGCTTGTTCATTGTCATCAATTGAAACAAAAATAACGCCATCTTCTTTTAAAAGTTGGCGAGCCATTTTTAATCTTTCGTTCATCATATTTAGTCAACCTGTGCGACTAAATTTGTCTCTATAAATAAATTTAGATGCCTCAACATCATCTTTTTCAGAAAGATTGTTGCCATCATCTTTTGCACTTTCAGTATTATAAGGAGGATCAATATATATTAAATCGTAATTATAATCCCCCCCCCCTATTTGTTCTTTTTGTCTTTCTATTACAAGTAGGTTTTTTAATGCATCATAATTTTCGCCAATAATTAAAGTGTTTTTATTAGTATTCAATAAATCATTTGTAAATGATAATTTTCCTTGTTTTTTTAATATTGCAATTTGTTTTGTATCAACACTAGGCGCCGCATCAAAAACAAAGCCCGTTTTAACTCTTTGAATCAAAAGTTGAAAAACATTGTCCAAGTTTTCTTTATTTGAATTGTTTGTGTTAACTAAAATTTCTTTTATTAATTTCTTTTGGTCTTGATTTAATAAAAAAGAATTCAAGTTATCTATATCATCTATATAGTTTTTTAAATCCTTCATGAGTTGTTCTCCTTGATATACCTAAATTTAAGATTTATATAATAATTTTAAATTATTTGATTACTATTTCTGTAATTTCCGACTTATTTCTTTACTATTTTTTAGTTAAAAAATTAAATTTAATAATTTAATTAACATATTTAACATATTTTTTTAACCTTAAATTTAAATATATTTGTAAAAAAATAAAAAAATTTGAACTTCATTTTAATAAAAGAGTATTATTAATAAAAAGAAACGCAAACATGCGCATATATTTCGATATATGGCATTTTTATTTTATTGCTAAAAAGAAAGGGGGATAATTATGAAAAACAAAGAAATAGATGCTAAACAAGCAAAACAAGAAAGAGCAGAAAAATTATTTGGTCAAACACCAATAAAAAAAGCAATTTGAATTGTTGCTATTCCAAGTTTGCTTGCTGCAATTATGGCTGGACTATATGGATTTATTGACCAAATTTTTATTTTGAATTTGGTTCCAAAAAATAGAAACATCTTTGGAAATAATGAAAGTGAAATTGTTCAATTTTTAAATCCTGCCTTGCATAGTGGTGGATTTTTTAGTGATTATAAGATAATGATTGAACAATATAATTTACATTCAATTACGAAATTAGCCGAAATTACTCCTAATAGTATTGTTTCAACGGCGTCGGCAGTATTTGGCCCATTAATATTATTTTCTAATGCAATAGTATTTTTAGTACCTGTTGGAGCTTCAATTTATTACACTAAATGTGTAAGTAAGCAACTAAAACGGGCTGGCCAAGATTTATGGGCGACAATGTTTTGATTTACGGTTGCGCTTTCTTTATTTGCTACGTTAGTATCTTTTGTGTTTATATGAAGTGGATTACTAAATGTATTAGCAGGTGAAACTCATATTGATTATAATATTGCTAGAATAGCCGGCATAGATCCTAATGCTTTGCAAGATTATTACAGCGCTGCATATTCATTAAGTATAAAATGAGCAAAGCAATTTCTATATATTTATGCATCAGGAACAATATTACAAGGATTAACTCTCTATCTATCTTATTTTATAAGAGCAGAAGGCTATAACAGTTATGTAATGATTTGTGCTATAGTTGCAAACGTAATAAATGTTGCTTGTGATGCATTATTTATTATTTGCTTTAAAATGGGAGTTTTGGGTGGTGTCATGGCAACCATTGTTGGTTGAACATTTAACACAATTACTTATTTTGCTTATATTTCAATAAAGATTAAGCAAAATAAATCCTGGTTATCTCTTTCGCACCTATTCAAATTTAAATTTAAAAAAGAATTATTGGGACCAACATTCTTACTTGGTGCAAGTGGATTTTTAAGAATTTTTGGAATAAGTTTTTCATTTTTAATTATGAATATTTTAATTACTAAAACCTCATTTTCAATGCCTGATTATTTCCAATATTACTGAGCAAAAACTCAACCAATTATTATGTTGTTCTTGACATCAATATTTGGTATAAATGATGGCGCTAGAAGCTTATTTACATATAATTATTCACTAAGAAACATGGATAGATGTAAACAAGTATATAAATGAACTATATTGATAGCTATTCTATATTCAGTTCTTGTTTATATATTTATTGCCTTAACATCTAATAATTTGTGAATATGAGTTTTAAACATAGACCCTGATAGAATTGCAGGAACTAGTGTATTTATTAAGGTTATGTCGCTTAGAATAATTGCCGTTTCATTTGTTGTTGTTTCATTGTTAGCATTTCAAGGTTCAAATGATATTGCAAAGTCTATCTTCGCCTCAATATTTGAAAACTTTATTTGCTTTGTAATAATTGTTCCTATTGGATATGGAATAGCTTGAGGAATATTTAATCATGATTCTGCTAACAGAGTAATTTCAAATTGAATCATTATTGCTGTGTTTGTTTTCAACTGTGTATTTGCTTCATCAATATTGATGTTTTATTCATGATGATTTGTCTATAAGAAAATGCCTTATATCGATCAGGCAAAAGTTAGTTGAAGTAGAAGAATTGAACATAAGTTTTTTGAACACGCAGAAAAACAAGAAGAACTATACAATTCTCAAATGCAACAAGAACAAGTTAATGAAAGTAATAATTTAAAGCAATAAAAACTAATAAGAATTTATATAAAAAAACTTGCAAATTTGCAAGTTTTTTATTTCTTATCGTTAAACAATTGCTTTAGTTTTATTGTCTTAAATTTTGTTGTTGGGCTGGGTGTGAATTGTTTTTACTATTATAAGTTTTTACTGCAAAACATAATAATGCAATTAAAACACCTAATTGTGCAAGATTAGCAATTACTGAAAGTCCATATATTGCTATAGCAGTTTTAGCAGCGCCTGATAAAAACATTGCTGTAGCAACAATTGCTAAGACGAATGAAATTATAAACAAGGCAACTAACGAAATTGATAATTTTCTTGTTGTTATTTCATTTTTACTTAAGCCGCGAGATCCTGTTTTAAATCCATAAACAACTGTTAGAATAAAAAAGACGATGCTTGCTATTGCTAATAATAATCCAAATACTGGTACAAGCGAACCACAAATAATATAAATAACATCTATTCCTAATAAGATTGCTGAAAATATTATTAGAAGTTTTGCATTTTCTTTGGTTTTCAAGTTTTTTGAATTATTTTCCATTACTTATTTTCCTTTCGTTTTATATAAAGCATAATAATTATATAAAATTCATAATTTTTAAATATTTTAAAAATAAAACATCAAGCGCCCCAAAACTAAATTTTGTTTTAAATTCGTGTTGCTGCTTGATGTTTTTAAATAATTTTGTAATTTTTAGCTTTTATTGTTGATTGTCGGAATTTCAATTTTGGGGGCCTTGAGAGCTTCCATCATAGTTATTTGTTTCATTATTAACATTATTGTTATTTTTAGCGCAATAGACCTTAACTGCAAAACATAATAATGCAATTAAAACGCCTAATTCTGCAAGGATCAAAATTAAACAAATAATATATAGTGCAAATGTTGATGACTCTAATAAAAATGGATTATATTCATTTGTTGGGTATTCATAATTATATGCGCCAAGTGATAAAGATATTGCTATTATTATTGCAACAATAACCATTGCAATTGCTGCTGCAAACAATGCTATTAATGCAATGGATAATTTTCTTATTATCATTCGATTACTATTTTTTGGTTCACGTGATCCTGTTTTAAATCCATAAACAACTGTTAAAATAAAAAACACAATGCTTGCTATTGCTAATAATAATCCAAATATTGGCACAAGCGAACCACTAATAATATAAATAACATCTATTCCTAATAAGATTGATGAAAATATTATTAGAAGTTTTGCATTTTCTTTAGTTTTTAAGTTATTATTCACTTCATTCTCCTATTTTATAGCTTAGATATTTTTTAATTATATTAAAAATTAGCGTTTTAAAATTCATTTTTTTTAAAAGCATAAAAAAAGTTTGCAAATATTATTGCAAACTTCTTACTTATGAATTAATTATTTGTTTGTAATTCAATAACATTATTATTTGAAACTGTTAATAAGGCAACGTCACCTTCAACTAAATTTCCTTTTATGATTTCCATTGCTAATTTGTTTTCAATGTTTTTTTGAATATATCTTTTAATTGGACGAGCGCCAAAAGCGGAATCGTAAGCGTTATCAGCAACGAATTTAACTATTTTGTCATCAAAATTAATTACTAAATTATGGTTATTAATAACTCTTTTCTTTAATTTTGATAATTCTAGTTTTACAATATCGCAAACAACGGCAGGACTTAATGGGTTGAATGGCACTATTTCATCGATTCTATTAATAAATTCTGGTCTAAAACTTCTTAATAATAATGCCCTTACCCCATCAATGGTTAAACTCTTATTTATAATTTCACTACTTCCAATGTTTGAAGTCATGATAATTATTGTGTTTTTAAAGTTTACTACCCTTCCTGTACTATCTGTTAAGGTACCATTATCCAATATTTGTAACAATATATTTAAAACATCTGAATTTGCTTTTTCAATTTCATCAAATAAAACTATTGAGTAAGGATTTTGTCTAATTTTTTCAGTTAGTTGTCCCCCATTATCATAACCAATATAACCTGGAGGCGATCCAATTAATTTTGACACGCTGTGTTTTTCCATATATTCAGACATGTCTAATCTTACAATGTGATTTTCATCATCAAATAAAGCTTCTGCTAAACTTTTTGCAAGTTCAGTTTTACCAACACCAGTTGGCCCTAAAAATAGGAACGAACCAATTGGTCTATTTGGGTCATTGATGTTTGCTTTAGAACGCTTGATTGCATCAGAGATTAGTTCAATTGCTTGATCTTGGCCCTTCACTCTCTTCTTTAATGTTTCAGACAAATTCAAAATTTTTTGTTTTTGGTTTTCTAGTAATCTATTAACTGGAATATTTGTTCATTTTGAAACAATATCTGCAACTTCTTCAGCATCAACAACTTCTTTGATTAAACGATTTTTCCTTGATTGTATTTTTTGTTCCAATTCATCTCTAGTTTTAATTAATTCAGGAACTAAAACATACATAATTTTAGAAGCTTCAACATATTTTCCTTCACTTTGTAGTAAAGGCAATTTTCTATTCAACTCTTCAATGTTTTCTTTAATTGAAGAAAATTTTTGAATATCTTCTTTTTCTTGCTTCCAGCGTTTTTCAAGGTCAACTTTTTGCAATTTTAAGGTATTTAGTTCTTTATCTATTTCTAAAATTCTTTCTTTATTTTTAATAGCTGTTTGTTTTGTTTCACTATCATTTGTTAGAGCAGCTTTTTCAATTTCTAATCTAGCAATTTGATAATTAATTTTTTCTAGAGGTTCAGGCAAATAGTTCATTTCAGTTTTTATATTTGATGCTGCTTCATCAATTAAATCTATTGCTTTATCGGGCAAAAATCTATCCGCAATATATCTTGCTGATAAATTTGCTGCCGAAACTATAGCAGAGTCATCAATTTTAACTTCATGATATGCTTCAAATCTTTCTTTTATACCACGCAATATAGTTATGGTATCTTCAATTGAAGGTTCTGAAATTAAAATCTTTTGCATTCTTCTTTCAAGAGCTGGATCTTTTTCAATATATTTTCTATATTCATCCAATGTTGTTGCCCCAATTAGATGCAATTGTCCTCTTGCCATCATAGGTTTCAATATATTCGCCGCATCCATATTGCTATCGCCAGTTCCTCCGGCGCCAATTATCATATGAATTTCATCAATGAAAAGAATGATTTCACCATTTGCTTCATCAATCTTTTTCAATACTTTTTTTAGTCTTTCTTCAAATTGACCACGATATTGTGTTCCTGCAACCAATGCAGGCAAATCAATTTCAATTAATTGTTTACCTTTTAGGTTTTCTGGAACTTGATTTTCAACAATTTTCCTTGCTAGGCCTTCAACAATTGCTGTTTTGCCTACACCAGGTTCACCAACTAAAACAGGATTATTTTTAGTCTTTCTTGACAAAATTCTAACTAGTGATCTTATTTCTTCATCACGGTTTATAACAGGTTCTAGTTTGTTCTGCGTAGCTAACTCAGTTAAGTTTCGACCAAATTCTTTCAAGGGATCTTTTTCTTGTTCATAAGGTGTATAACTTGCTTCCATATATTGTCCTCCTTTTACAATAAAAAATTATACTACAAAAATTAGCACTGTGTTAAAAAGAGTGCTAATTTTTTAACTGATATGTTGCCCATAAAAAAATTTGCTATTAAAATAATTTTATTTTTTTATAAAAAAATATTTTAGTGAAACATTATGTTTTTTTATTCTATAATTAATCCGGTTATTTCGTAACCGATTGAGTTAAGATGAAAGACATTATAAATAAACGATTTTGATTTCTATTTTTATTTATAAATGTTGCTACGCTTTGCATTTTTTTAGGAATTGCTTATCTAAACTGAGCTCTACTAACAGGATATTTGGTAGGAGTAATCAGTTTTCTTATTTTTTTAAGTGGCTTACATTTAGTATTTAAAAAAATGAATGATTGAAAAGAAAACGCTAGTATTAAAAAAAACAAAAACCTCGCAGTTATTATTTTTTTAATTTTAAATTTTCTTGCTTTATTGATTATTGCCTTATTTGTAATCTTTAACTTACTTTATAAAAACAAACATTCAAATGCAAATGTTGCATTTGTACCTTTTAACGTAATCACAATGGCTATTCCATATACATTATTTAGTTTACAAATAATAGTTATGGAATTAATTAAAAAAATTACCACTAAAAGGAATTTAAAGAGAAGGGAGGAAAATGGATAGAATTTTACGTCTTCATTGATTTGGTGGTCCAGTTAATCCTGGCGAAGTAAGCAGTTTCAATGACAACCAATTATTTACTTTAATTATTTTAGTTGCTTTAATTTTTATTATTTCAATACTAATTTTTGTAGCAATAAAAAGACAAAAAGTTGATAAAGCACCTAATACAGTTGTATTAATGGTTGAAAGTATAATAAAAAACAGTGATGAATTTATTTCTGAAACCCATGGCAATAAATTTGACAAAATGAATCCTTATTTTATTTCATTATTTGCCTTCTTGATTTTAGGAAATATGCTTTCATTAGTTGGTTTAGCCCCAATTGGAACTAGTTTGTCAGCAATTTTTGCTGCCACTGTTATAAGTTGACTTGGAACACACATCATTGGATTCATATATAAAAAATTTAAGTATTTATTTAAAATTCTTAATCCCTTAGAAGCATCATCAAACTTTTCACCATTAATAAGTTTAACTTTCCGTATGTATGGAAACATACTTGGAGGAGTTGTATTGGTTGCACTAGTAAGTTTATTCTTAAATAATATTTGAACCAAAATTATTGGCGCTAACTTAGAATCACCTTCAGCTGAGTTAAACCCTATTGGGTTATTGATCTTGCCAGCATTTAATTTATATTTTGATATTTTTGATAGTTTCATTCAAGCATTTGTATTTATGATTTTAACAATTTCATATTGAACGCAATCTGCTGAAGAAGATATAAAAGAAAAACATAAAGACAAAATAAAAGAATGAAAACAAGAAATCTTAAAAAATAAACAAAATAAAAATAATATAGATAATAAAAATAACGAAACCTCAGTAGTAGAGGCAACTATTTAGGAAAGGAAAAAAAATGGATAAATTAGAAAATATTGTTAATGCATTCAATACAGCATCAGCAGGTGTTAATAGTAAAGAAGCAGGAGTGAACTACCCTCTAGCATATGGTTTAACAATGTTAGGAGCAGGTATTGCTGTTCTTGGCTCAGGTTTAGTTTCTATTGGTCAAGGTATAGCCGTTGCTAAAGCCTGTGAAGCTGTTGGAAGAAATCCAGAAGCATTTTCAAAAATTCGTACATTGTTAATTTTGGGTTTAGGTATTGTTGAAACCGGATCTATTTATTGTTTAGTTATTTCATTATTGCTAATCTTCGTTTAAAAATATGTTTCAAAATTTTGCAAATTTATTAAGCCAATCGCAAAATGCAATTGCAGCAGCTAAGCCATCTGCTTCTGCTAAAATTGCAGAAGAACTAAACAAAACTTTTAATGGTTTAACATTCAATTGACCATTCTTTGTTTTTTCATTAATAAGCTTAGGCCTAGTTGTTTTGATTATTACTTTATTAGTATATAAACCAATAAAGAAATTAATTAAAAACCGCCAAGAACTTATTCAAAAAAATATTGATACTTCAATATTAGCAAAAGAAAATGCTTTAAAAGTTCAAGAAGAAAATGATAAAAAAATTATTGAAGCTAATAATCAAGCAGCTTTAATAATTAATCATGCTAAATCGGAAAGTGAAAGAATTGTCAACGCAAGCACGATTGCTGCTAAAAAACAATCTGAACTTTTATTAGATCAAGCACAAATTTTGATTAATAAAAAGCAATCTGAGCTAGAACAAGCGCAAAAGAAAATAATAGTAGAAAATGCTGTTGAATTAGCCAAGAAAATTATTGGCCGTGAAATTAGAAATGAAGATAACATTAAATTAATTAATGAAGTCTTAGAAGATTAATATGCAAGATTTTAATGAAATCATTTATAACTGAGCATATGCAATATATGATCTTACAAATGAAAACAAAAATTTGGCATTAGTTTCTAATGAAGCTGCTGAAATTACAAGACTTTTAAAGAAAAATAAAGATTACTTGTATGTTTTAAATTCTTATGATATTGACGAAAATGAAAAATATCGTCTAATTGATGAAGCATTTTCAAAATACAATTTTTATTTAGTAAATACTCTTAAATTAGCAGCTAAGCAACATGTGATTAAATACATTATTGTAATATTAACAAAATTTGTTGAACTTTCAAATGAAAAAAGAAACATTAAATACGGGTTTGTTTATACAATATCGCCTTTATCTGAAAGAGAATTAAAAAGGTTAGAAGTAAAACTTTCAAACGAGTTAAAGGCTAATATTCATTTAACAAATGAAATTGATCCCAAATTAATAGCGGGAATCAAAATAAAAGTTGACGATTATTTAATCGATAATTCAATAGTCAACAAATTAAATGACTTTAAAAAAGTCGTAGAAAATAATTAAGGAGGGAGGAATATGTCATTAGAACCAAATGATTTGTCAGCAATCATTAAGGCGCAAATAAAACACTTTAATGAAGACATATCTAAACAAGAAATTGGGCGTGTAATAACAGTTGGTGATGGTATTGCCTTAGTAAGTGGCTTGAGCAATGTAGAATATGGCGAATTGGTAAAATTCGAATCTGGCGTTATTGGAATGGCCTTGAATCTTGAAGAAGATTTAGTTGGTATAGTTGTCATGGGTGATGACCGTAACATTGTTGAAAATAGTACAGTTTATCGTACTAAACAAGTTATATCAACACCTGTTGGTAACCATTTATCTGGCCGTGTGTTAGATGCACTTGCTAACCCAATTGATGGAAAAGGTCCAATTCAATATGAATATACTAGACCAATTTTTAAGATTGCACCTGGTGTTATGACTAGAAAAGAAGTTAATCAACCACTTGAAACCGGGATTTTAGCAATTGATGCTATGATTCCTATTGGTAAAGGTCAACGTGAATTGATTATTGGCGATAGACAAACAGGTAAGACCGCTATAGCCATTGATGCAATTTTAAATCAAAAAGGTCGCAATGTTTATTGTATTTATGTTGCTGTTGGTCAAAAGAATTCAACTATTGCACAAATAGTTGATCAATTAGCAAAACATGATGCATTAAAATATACAACAATTATTTCTGCATCAGCATCAGACAGTGCTCCTTTACAATATATTGCACCATATACAGGAATTACTATTGCCGAAGAATGAATGGCAAGAGGTAAAGATGTTTTAATTATATATGACGATTTGTCAAAGCATGCTGTTGCATATCGTACATTATCATTACTATTAAGAAGACCACCTGGACGTGAAGCTTATCCTGGAGACGTCTTTTATATTCACTCACAACTTCTAGAACGTGCAGCTAGAGTAAATTCCGATTATGGAGGCGGTTCAATTACTGCTTTGCCAATTATTGAAACTCAAGCTGAAGATATTGCAGCATATATTCCAACAAATGTTATTTCAATAACCGATGGTCAAATCTTTACTAAGGAAGCATTGTTTAACTCTGGCCAACGTCCTGCTATTGATATCGGTTATTCAGTTAGCCGTGTTGGATCAGCAGCACAAACAAAATTAACAAAAAAGGTTGTATCAAGTCTAAAATTGGAATTAGCACAATACAATGAAATGTTAGCATTTGCTCAATTTGGTAGTGATCTAGACCAAGCAACTAGAACTGTTTTGGATCACGGTGCTAAAGTGTATGAATTATTGAAACAACCTCAATATTCTCCATATTCTTCAATAGAACAAATAATTATTCTATTTTGCACAAAATATCGTTTAATAAATGTAATTCCAAAAGAACAAATATTAAAATATAAAACTGATATTTTAAATTACTTTAGAACTAATCCTAATGCTATTAATACATTAAGAGCCGTTTCTGAAGCAAATGATTGAACTAACGAATTAGTTGAAAAACTATATAATGAAATAATTAGCTTTAATGAATTATTTGTTAAAACTATTCCATTATATAATAAAGATTTATATCAACCAGTACCTGAATTACCATGGAAAGTTTGCAAAAAATAAAACACAGAATTAATTCGGTTAATTCAACTAAAAAAATTACCAAAGCAATGGAGCTAGTTGCAACTGCTAAGTTTGCTAAAATTAAAAATCAATTTGAAAATATCAATAATTATTATCAAAGTGTTGAAAGAGTTTTTTTAAACTTAATTAAAAATTCTGATCAAAATATTGATAAATTATTGAACAGTGCTTCTTGAAAATTCAAAAGCAAAAGAAATTTATATATTGTATTTGGCAGTGATTTAGGACTATGTGGTCCATACAATTCGGAAATGTATAAAAAAATTACTCAAACTGTTGGAAAAGAAGATATCCTAATAGTAGTTGGTAGCAAATTATTGAGTTTAATTCAAAAAGATAATAGCCATCATATTATTCAAATATTAACTCAAATTGGTGACAATCCAAGTTACGAAATCGCTCAAATATTGTCAAATAAGATTTATCATGTTTTAGAAATATCATGGCTAAAATCTATTAAACTTATTTATACTCAATACATTAATCCAATAAAATCTGAACCAATAGTAAAAGAAATTTTTCCAATTTCTATTGATACACTATCAACAAATAATGATAACAATTTAGCAGGGGAAGAAAGTTCAAAAAGTATTGATCCTTCTTGCTTTGAACCAAACCCTACTGTAATATTGCAAAATTCATTTTCAATATTCTTTGAAGCCTCTATATACAATGCCTTAGCAAATGCTAAGCTTGCAGAAATGAGTCAAAGAAGAACAGCAATGGAACAAGCAAGTGATAATGCACAAGATCTAATTAATGAATTAAATGTTCAATACAATAGTTCTCGTCAAGCAAAAATTACACAAGAAATTACTGAAATAATTAATGGATCAAATTAAAAAGAAAGGAGTAAAAATGGCAAATAAAAAAACTAATGAAAATCAAGAAATTCAAGAAGAACAAAACCCAGTTGATGAATCTAAAAATTCAGAAAATTATGGCGTTATTAGCCAAATTTTGGGATCTGTTATTGACGTTAGATTTGAAGAAGGACATACTCCAAAAATCTTAAATGCTTTAAAAATTATTGATGAAGACAAAAAATTTAGCAATGAACAAATTCTTGAAGTTGCTCAACACGTAGGAAATGATACAGTTAGAACAATAGCAATGAACTTGACTATTGGTTTAAAGCGTGGTATGAAGGTTCTTGATTTGGGTTCTCCAATAAAGGCGCCTGTTGGTAAAGAAGTTCTATCTAGAATGTTTAATGTTCTTGGTGAACCTATAGACCAAAAAGGTGGCGTGTTCAAACACAAAATGCCTATCCATAGAGCAGCGCCAAGCTACGAAGAACAAAAATCAACACTTGAAATATTTGAAACAGGAATTAAAGTTATTGACTTACTTGTTCCATATATTAAAGGTGGAAAGATAGGTTTATTTGGAGGAGCCGGAGTTGGTAAAACTGTTTTAATTCAAGAATTAATTAACAATATTGCAAAACAACACGGCGGTCTTTCTGTGTTTGCAGGTGTTGGAGAACGTACAAGAGAAGGTAATGACCTTTATCACGAAATGCAAGCAAGTGGTGTTCTAGAAAAAACTGCTCTAGTATTCGGTCAAATGAACGAACCTCCTGGAGCCAGAATGAGAGCACCATTTACTGCTTTAACTATCGCTGAATACTTTAGAGATGTTATGCATCAAGATGTGCTATTGTTTATTGATAACATATTCAGATTTACACAAGCAGGTTCTGAAGTTTCTGCATTGTTAGGCCGTATGCCTTCTGCTGTTGGATATCAACCAACATTGGCTGTTGAAATGGGTCAATTACAAGAAAGAATTACCTCAACCAATAACGGATCAATTACTTCTGTTCAAGCTGTTTATGTTCCTGCCGATGACTTAACAGACCCCGCACCAGCAACAACATTTACACACCTTGATGCAAAAACTGTTTTGGACCGTGATATTGCTGCTTTAGGTATTTACCCTGCTATCGATCCTTTGGCTTCATCATCAAGAATGGTAGATCCAAATATAATTGGTCTTGAACACTATAACACTGCTAGAGCAGTTCAAAATATTCTTCAAAGATTTAAGGAATTACAAGATATTATTGCTATATTAGGTATGCATGAATTAAGTGAAGAAGATAAGAAAGTTGTTGCAAGAGCAAGAAGAATAAGAAACTTCTTATCTCAACCTTTCTTTGTTGCCGAAAAATTCTCTGGTATTAAAGGACAATACATAAAATTGTCAGATACTATTAGATCATTTAAAGAAATTTTAAGTGGAAAATATGATGAATTGCCAGAAGAAGCATTCCTTTATGTGGGTACCATTGACGATGTTATAGAAAAAGCTAAAAGACTTGGGGTTAATGTTGAAAAATAATGGAAACTCCTAAGATACATATTATTATTAGTACTCCATTAGGAATATACTTTGATAAAGAAGCAAGTATTTGTACATTTACTACTACTGAAGGGCAAATTGGAATTATGGCAGGCGCAACTGAGTTTATGGCAGCCTTGGTTCCTTCAAAAATAATGGTTAATTTTTTAAATTCGCCTGATTCTAAAGTTTTTTATATTGATAAGGGAATTGTTCAATTTAAAAATAATGTCTTATCAATAATAGTTAATCAAATTGATACTAAAGAAATTGAATTGGATCATAAATTTAGCCCTGAAGATCAAAAGAAATATACAATTATTGAAGAAGTTACTTTGAAAAAGAGCATTTTGAAAAGTCAAACAAACAATAAATAATTAATTTTAAAAAAGTAGGAATTTAAAATCCTGCTTTTTTTATGCTAATTTTTAAACTAAATTTTTATAATGAAAAATTTATCAAATTAATAGTTTGATTAAAAAGAAAAATTTCTGATTTCAATTTTTAAATTTTAAAATATTTCGGCCATTTTTTATATGTTTAAAATTCTTAAAATTTGTAATTCTTTTTATAAATTTTAGAATTATAATTCCACAGTACAACAAAAAACAAATAAGGAAGGAAGATAATAACATGAAAAAAACAGCAAAAGCTTTATTAATAATATGCACAACTATAACACCTAGTTTTGCTATGCCATTAATATCGGCGGGATGTAATAAAACAACAAACCATGAAACAAACAAAGAAAATACAATTATAGATAAACAGGCCCCTATTAAAAAACAAGAAAATGATTCTAATAACCCCAATAAGTTAGAATCCAACAGTGAATTAGCAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGACAAGATTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAATAACCTTGATAAAAAATTTAAAATAGAAATTTTAAAATTAGAAGCAGACAAGAAGAATAATTTAATATCGATCTCGTATAATATTTCGTTTAATGATGGAAATAAAATCATTAAATTAGAAAATCAAAAATATTCAATGGCAGGACTTTCAACAAATAATGAAAGCTATTTAAATGGATTATTAAATGATAATGACACAATATTTAATTTAACAACTAATTTCAAAAAAGGTAATGAAATAGATGCATATCATTTAGCTAATAGCCAAGGCTCTATTAGTTTATTGGCATCTAATAAAAATGTTTATGTTCCCAAAGATCTAATCTTAGAATATACTTGAAATGAACAAAATGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACTAAGGACAATAAAACTATTACAAGAAAAAATGTAAAAATTGCTAATAAGATTGCCGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAATTAACTTTAAATTATAAAGATGCTGACAAGACTTCAATAGATTCTTTTAGCGAAGGTGATATTGAACAAACGTTAAAAAATAATTTTATTGTTAAATTTGAAAAAAGCAGTGAAGAAGAAACTAAAAAATTCTTAGAAGACAATGGAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAATGTTAATCTTCAAATAACATTAAAAAATAAGAAACTTGTCAATGCAAATATTGAAGTTGCAGGATTTAAAGGCATTGAAAAAGTAAGCGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAGTTAAAACCTTTAGTCAAAGTCGTTTAAACAAATTAAATTTATATATTAAAGATGAAAACGGAAAAGCTCAATTAGAAAAACCTTTAAATTTTGCAGAATATTTTGCCAAAAACGGAATTAACAATTTTGATAAAAATTATATAGTTAAATATGATAAAGCCGAAAAGAAATTAATTACAACATTAAAATTGAATGAAAAAGAATACACAATTGAAACAAAATTAACGCATGAATCAAAACCAGATGAATGGGTCAACGAAGTAGGTAAGGAATTTTTAGGAAATCCAAATGAAATTTTGGCAAGTGATTTCTCAGCTACAAATTTAAAATTGAATAAAAATGTTGGCGATAGTCTTGATAGTTTAAATTTTATTAAATTATCTTCAATAAAAAGACCTTCAAAAAATTCAGAGGAATTAAAGCTCAACGAAGAAGGTAAGGCAACGTTAATTTATGAATTTAGAAATGTATATGATAATTCAATATATACAACAGAAGTCGTATTTGAAAATCTAAAAAAAGTAGAAAAAGCTGATTATAAAAAACAACACGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTAAAAGTGGTTGGGCAACTGCCGAAGAAATAAATGAATTGGAAACTTTTTTGCCAAGTCAAAAAAAATCTAAAAAAAATAAGAGAATAAACATTGAACATTATATAAACAATGGCAATATAGAAATACTTGTTGGAAGAGTAATTACTAAAAAAAATAAAATTACTTTCGGCGATACATTGAACAGACAAAAGGAATTTGAAGAAATAATATTTGATGAAGAATTTGTTGAAGCAGTTAAGAAATCATCTAATAATAAAAGCGAAAATAAAATATTATCTAATGTAATAACAGAAGAAAATTCTAAACATAAAAATCAAAAGAATTGAATAAGACTTACAAAACACGAAGGAAAATGATGTATACTATTTGCATTCATTGAAGATACAAAGTCAACACCATATTATGTTCAAATAGAAAAATAGTCTTATAAAATTTTATTGCAATAATAAACGCAATAAAAACATTTAAAGAGTAGGGGACTGGCCCTACTTTTTTATATTGAAACAAAATAGTAAAAACATAAAAAATAAAGTCAAAAGTTTTTATTTTTAACCAAGGAGTTTAAAATATTTGTTTTTTGTTAATTTTTAAGATGAATGCTCATTTTTAATCACCTTAGCAAATAAAATTATTTTAAAATCAAAAAAAGGAAATACAGAATATGAAGAAATCGAAAAAAATTTTGTTGACCATGGGAGGGATACTTCCCTTGGTTATTTCAGCGCCATTAGTTGCTGCTGGATGCAAGGAGCCAAATAAACCAGAAGTTAAACCAGAGGATCCAAAGAAACCTGAAGGTGAAAAACCCGGAACAACTCCCGAAGTTAAGCCAGAATCTAACAGTGATTTAAATGATTTAACAGAAGCAGTTAAAAAAGCTAATTTTCAATATAAGGATATTGATAAGGTTTCAATGTTTGACAAATTAAACAAAGAAAATTTATCAAACAACTTCGATAAAAAATTCAAAGTAGAAGTTTTAAATTTAGAAGTAGATAAGAAAATTAATTCAGTATTAATTACATACAACATTTCGTTCAATGACGGAAGTAAAGACGCTAAATTAGAAAATCAAAAATATTCATTGACAGGACTTTCAACAAACAATGAAAGCTATTTAAATGGATTATTGAACGATAAGGATACAGTATTCAATTTAGGTGATGCTTCTAATGATGATAGTTTAACAACAGTTGAACAGTTAGATGCATATTACTTAGTTGATGGTCGAGGTTCTATTAGTTTATTAGCATCTAATAAAAATGTTTATGTTCCTGAAGGTCTAATGTTAGAATATACTTGAAATGAACAAAACGAAACAAATAAAAAATTCAATGGTGAAGTAGAACTTGATGTTAGTGTTAAGACTAAAGACAATAAAACTATTACAAGAAAAAATGTAAAAATTGCTAATAAAATTGCCGAAGGCGTTTCAGATGATTATTTAACTAAATATAAAAAGTTAACTTTAAATTATAAAGACGCTGACAAAACTTCAATAAATTCTTTTAAAGAAGGTGATATTGAACAAACATTAAAAAATAATTTTATTGCCAAATTTGAAAAGAGTAGTGAAGAAGAAACTAAAAAATTCCTAGAAGACAATGGAATTAAATATGAAGTTAAGAGCATAACTGAAATTTCTCTTGAAAAGAAAACAATCAACGTTAATCTTCAAATAACATTGAAAAATAAGAAACTTGCCAATGCAAATATTGAAGTTTCAGGATTTAAAGGCGTTGAAAAAGTAAGTGATGAACAAGTTAAAAAAATAGTAGATAAATTGACCTTTGGTGATGTTTATTTCCCTGAAAAAATAAAAGAAATTTCAAAAGATGAAACATTTTTGAATGATTTGCCTTTATATATCAAAGATGAACATCAAAAAACTAATCCCGAAAATTTTGTTAATTCTGAATATTTAATTAAAAATGGAATCACCGAATTTGATAAAAATGAATCTGGGCAACTTAACTACAAAGTCAAATATGATGAAAATGCAAAAAAATTGTCTGTAACATTAAAATTAAACGGCAAGGAATATAATGTTGAGACAACATTAACTCATGAATTAAAATTAGATGATTGGGTAAATGAAGTTGGAAAAGAAATTAATGGCGATTCAAATACCATTCCCGTAAATGAATTTTCTAATAAGAATTTAAAATTGAAAAAACAAATAAAAGAATTTGTTGCAGGTTTAGACTTTGTTCAATTAAATTCAATAAAAAGAGCAAAATCTTCTGAGGCAGAAAAAAAATTGAATTTAGAAGGTAAAGCAACCTTAATTTATGAATTTAAAAATATATATGATAATTCAACATATACAAAAGAAGTCGTATTTGAAAATCTAAAAAAAGCAGAAAAAGTTGATTATAAAAAACAATATGCCTCTTTAATACAAAAAATTAATGATAAAAAAATTATTACTAATAATAAATGAGCTTCGCAAGAAGAGATAAACAAAATAAGCGCCTTTAAAAACAATAAAGAAACATATTCTTATAAAGATAATGAAGGTAAAGAACAAAGTGAAACATTATCTGGCAGATTTAAAATTCAAGGAAAATCAGGCCATTTAGAATTGCATTTTGGAGATAATGGTAAAGCTCGCGAATCATTTAAAGAGTTAAAATTTGACAATGATTTTCTTGAAGCAATAAAAAGTAAAACATTAGTTAATGTTGTTAAAGGTTCTAAAAATAACCAATGAGTTAGATTTACACAACACAACGGCAAATGGTGTATAGTATTTGCTTTTAAAGATGATCCAGAACTTACACCTTACTATGTTCAAATAGAAGAATAATGAGTTAAAAAATAAAGTTCATTCTATAAAAAGAAAGGACTTTATTTTTTATTTATAAAATAAAATTTATGTATTATAATAAATTTTTATAAAAAGCAAAAGGCGGACAAAATGACTAAGAAAAAAATATTATTTAAAATATTTGCTATGGGCACTATCCTAGCCGGAGGCGTTTTTAGTTTAAATTCATGTATTTTTAAATCAAGTGATACAGAAAATAAAGATAATAGTTTTGAAAATAATAGCAATAATGATCAAAGCAACCAACATGATTTAAGCACCGAAGATAATAATTCAAGAGATTTGATAGTTCCTGAATCAAATAATAAGCCCGAAAATGATTCTATCCCTCAACCTGAATCAAAACCAAATATTATCCCAACGCCTGAAATAACGCCCAAACCAGCGCCTGAAATAACGCCCGAACCAGCGCCTGAAATAACGCCCAAGCCAAATGAAAATGACTATAATAAAGCCAATCATAATCAAACTCATTTTGTAAGGCCACCTTTTATTGCTAAATTTAAAATTAAAAATACTGATTTTTCAATAGCGACAATTTTTAACCATTTAGATAGTCCTGGTACTAGAAAAAAAGGAGAATACATTGAACCAAAGCCATCGATTTTATTGGCTGATAGTTCAAATAGACAGTCAGATTTGAATAAACAAGGAGCCCAAGAAGTAAGTGAATTTCTAGGCTTAATAGATGTTATTAATGAAAACAAAGATTTGGCCAATTTTGTTATTTACGGTGGCGATACTAATATAAAAAATGAAAACTTTTATTTAGCAAGAAAATATCCCGAAAATATTCAATTTACTTTAGATAGAGACTTAAAATTAAAAAAATATGACCAAAAATTTCTAACATCGTTAGGAACAAGTGGAAAATATTCAGAACAATACGACAAAATGTTCTTTATTAATCAATTGCCCAATACATTCAAACCAAAAATAATTACCGATGGTCTTTTACCTTATAAGGTTGATATTTATAGAACGTTTAATGATGTTATTTCAAAAGCAGAGCTTGAAAGCTTAAAAGGATGAAAATCAAAAGACAGTGATAGATCTACAATTAGAAGTCAAATTTCAGACCATGCTCCAGTATTTACAGATGTCGTATTTTCAAATCCAATCGAAACGCTAAAAGTAAATGAAGCATTAAGAAAAAACCAATCTTTATCTAAAGAGCAAAACACTATAAGAATAGCTCATTGAAATATACTGAATTTTGGCGGTCCTAATCAAGATGCTAAAACAATGGCTATTGCTACTACAATTTATAAGTCCGGTTTTGATATAATAGGCTTAACTGAAATTAATGATGGAAGAGGAAACTATGTGCAAAATATAGTTGATCTTTTAAATGAATATGCAGGAAAAGAAAGATATAAATTTGTATTACAAAATAAAAAGGATACAAATATAAGGCCTGAATATTTACAATCAAATAAGTTTGGAAAAGCACAACAAGAACAAGTTGCAGTGATTTATGATAGTCAAAATTTTGAATTTGTTTCAAGCCGAAGCTATTTAAAACCAATTAAGTATTACAAAGATAACTAAAAAAACTTGGATGCTTCCAAGTTTTTTTAGGCTAATAATCGCCTTGTTTAATTTTATTTAATCCACTTCATTTATTAATTGAAGTTTTGTTATTCATATTCATGAATTTTCTATAACCACCTTCTTTGATAGTCATTAGAATATATTCTTTTTTAGAAACTTCATTAGGTTTTCCATTTTCTTTAACTTTGAAGTTTACGTTTTTTAATTTACTACTTTCAGATTTGTTATATCCACTTGTTACAATTAAATAAGTAATTGATTCAGGACCATAATTTTCTTCTTTATAGTATTCTCAAGAATGAATAAATCATCCTCCAGTTTCATTATAAAGATAGTTAGTAAATTCTCTTCTTCTTTCAGAGTCTTTAAAATAAGCCTGCATTCCTGTAAATTTACTTGATACTCCAGGAATTGCTTTAAGATTAGTTCCTTCTTGAAATTCTCAAAGTATTGCCCGTGTTCCTTGATATAGGCTCAATGTTGGCGCATTTCATTTTGAACCGGGTTCCCTATCTTTTTCCAATGTTTCTCTACCAAACATTTCAAATATAGCAAAATCTACTGCTACATTAAAACGATATACTGCTTTTCATGCTTCGGATTGTGCCCTAACTTTATATGTTGATGAATTTGCATCCTTATCATTATTGTATGCCTTTGCAAGCAATTTATAAATTGTATCAAGTAATTTTAAAACGTCTGCTGAATATTGAATATTATCGGGGTCATCTTTATCGCTGGCTTTTGAAGGTTTGAAAATGCTTCCCAACGCATTTGAAACCGAGCTATTTAAAGTATTCATATTTGAACGTTTTGATTCATCTGAATTATTGTTTAGATTTCTTAATGCATTATAGATTACAGAGTCATTAAAATTACTTTTTCCAAATACTAAATTGATTAATTTAACCAAAGCAGTTTCATTGATTGGTTTATCTTCACCTACTAATTCAGATAATACTAAAACATCATTGAAGCTAAATGCTGATTTAATTTTTTCAATTGCATCATTCAATCATTTAACAGGATTTAAAATATCAAATTGTTTAGCATAATTACTTATCAATCCTATTGAACGCTTAATGAATTTCCTTTTAATAAAATCATTATTTAACAATGCTGGTACGATTTTTCCAATTAAGTCTTTTAGGAATTTTTTATCTTCATCATTTCCTTCTATTTTTAGATAATTTAAAATACTTTCAACAATGTGATTTGTAATTTCATCATCAGTTGCAAGATGCTTAATAAGTTCAGAAGAGTTTTTAATTAGATTATCTTGAATTCTAGTAATTGTTTTAGATATTAAGTCCCCAAGGTTGGTCGATTCAATTTTTGTTCCTGCTAGAAAATCATTAACTACGGCTTCAATTAAGCTTTTAATTTCTGGAAAATCAATGAATTTTTTACTTATTAATTCCAACTTATCTTTTGGAATGATATCTTGTTTAAGGCCATCTCAATTAAATTTCTTAATTTGATCCTTCAAAACATTATATACAAAATCAACTAATGCTTCTTTTCCGTTTTTATTATCTGCTTTTATTGATCCATTTAGAGCTTTTAAAGCATTGTAAATTAATTTTTCTAAATTCTTTAGGAATTGTTCGTGTTCTTTATCTTGTTTAGCTTTATCTACTTCGTTTAACGTTGTAGAATTACTTTGAGCAGAGAATGCTTGTTTAAATTTATCAAGATTGTCAACTATATATGAATAAGCGAAATTCAATTCATTATACAAGACATTTTTATCAATATTTTTATCTAATATTGTTTCAAATAATTCACTTAAATTTTCTGTTGTTAAATAGTTATGGTATTCAATCTTTTTAATAGATTCAAGAAGTTTGGATGCCAAAGCATTTCCATTGAAATGACCATTATTGTCAAGGAAATCAACATCAATAAATAAATCAATGAAATCTCCCAATATATTGCTAAATACGTTCATTGAACCTAGTTTGTTAATTAATCTAGTCAAAAGATTTGCAATTCCTTGAATTTGAGCGCTACTTGAACTAATTGAATATTTCGTGTTGAATAAATTCAATGCATATTCAATAGATTTGTTAATTAAGTTTTTGTTAGAAGTCATTTCATTTACTGCGTTTTTAATAAATGCTTTAAATTTATCTTTATTTGCTGACAATAAATGTTTAAATAAACCATTAAAATCACTAATAGAAGCTCATTGATTGTTATCGCCTAAATCTATCAATGAATTTAATAATGTTAGTATAAATTCTTTTGTATTATTTGAATCAAAAATTACCTTCATTAATGTTTCAACTATTTCATTTCCTAATTCAGAAGCTTTTGAATCAATTTTTAGAATTTGTTCTTTTATTTTTAATAAATTGTGTTTGATATATTGTTTGAATTTATTACTATGCAAGAAAATATCAACTGAATTTTTTAATTCATCTTTAATTTCTTTCTTTGCCTTATTATCTAATATTGAAAATACGCATTTAAACAAATTAAAATAAACATTAATATTTACCTTGATTTGTCCATCAAATCCAACTATATTTGTGTTTGAAGAATCTAAATTTTTATGTTCAGGAAGTGATGGAGATTCATTGCTATAATCTGGAATAATATTTTCAAAGTTCTTGATATTTTTTAATAATAATAATGCAATATTTTTTATATCTTCAGATTTTAAAGATTTAACAAATTCTATGATTTTGTCGTAGTTTAAATTATTAATAATGCTTACAATATTAATTAAATTATTTCAATCGTACTTTTTAAATTCAAATTGATTCTTGTTTAGATTATTGACAGAGTAGTCTAAAACATTTTCAATTAAGCCATCTAGAATATTAAATTCTGGCAATTTTTCAATGCCTAGCGAAATTAATTTTAGTAATGTTTCTTTTGCATTAACAGGTCATGTAACATTCTTTAGATTTTGTTCCATCAATGAAATTAATAATTTTGCTATTGAATTTTTAATATCAATACCTAAGGTTTTGTTATTGATAAATTTATTGAATATTTCTTTTATTTTTTTAGTGACATATTCTTTATTATTTGAAATAAATTTGCTTATTCATTGAGAAATATTATTTAAATCTTTTAAATTATTATCATCAAATAACTTTTTAATTAGCTCTTTTATTAAAGGTAAAAGATCTTCATTGTAACTTGTTTCAACAAATGATTTTATATATGAAATTATTTGTTGAAGGTTTTCTTGTACATCACCCTTAAAGCTTGAAATATTTATTTCATCAATGTATTTCAAAATGATTTCTTTAATATTTATTTTGCTTGCTATGTAATTTATAAATGTTTCTTTTTCTAAAACAATCTTTAATATTTTAATTAGTTCGACATCTTTTATTTGGTTAATTTCTTGTTCTTCATATTTCTTTGTTAAGAATTGTTGAACATCCTTAATTAATTCACCATTATTGAAACTAAAATAATTCTTAATCGTTTTTGCAACGGATTCAAGAATAGAAGCTCAAGAATATTTATTTATTGACTTCAATTCATTTGTTAATTTAATTTTTAAGTTTTTATATAAATTAGATTTCAATGAAGTAGGTAAAATCTTGTTGAAATAATTAGTTAATTGGTTAATTTCATCAGAATTCAATTTTGTTTTTGCTTCAATTTCTAAAAACGCAACTAAAAATTCAACTAATTTTTGGGTTATATCTTTATTTGATAAAACATTTTCTAGTAATTGTTCTAGCGCATCAAAGCCTTTGCTGTCTGCGAGATTTTTCAATATTTTATTGATAAATTCAGGCAATGTTTGAGCATCATATTCACTGCCGTTTTCAATTAAATTTTCAATAAATTTGAAAACTTTGTCAATTAATTTATTATCAATAATCTTATCAAATTTATCGACAATAAAATTGGCAAGATTATTATCATTTGTATTGACTTTACTTAGAACTTCATTATGAACATTTTTTAACTCATTATTAATTATTTTTGTTAAATATTGTTTTAAATTTTCATTTTTTAATAACTTTTGAACTTCTGGTTTCAACTCATTGATAATTGTAGATTTAGATGAAGCTAGTTTATTTCATAATTTTTTAATTAGTAAGAAGATTTGCTCAAAATTTAAATCATTGTCTAATTTAATTGGTTTTGAACCTTCATTTTGTTTTTGTGTTGTTGTGTCGTTGTTTGCTAAGACAACATTTGCAAATTCATTTAAATTCTTGATTAATATCAAGAATAGAGTTTTTACATTTTCTTCATTTAATGTATCTATCAATGAATTTAATTGTTCGTAGTTAAATAAATGCTTAATTAGATTTTTTAGATCTAAAGCATTGCTTCAACTAATTTTGTCTAAATTAAAACCATTATTTTCAATGTATTTAGAAATATACGAAGCGAATGTTGATACTAGCAAGTCAACATTTTGTTTTTGCAAGGCAAAATCAATCAATTTTGATACTAATTTTTCAATATTGCTTTCACTATCTATTCCAGCAAGAAATTGAGGATATTGTTTCTTTAATAATTTAAATAAACCACCTTTAATTTCTTCGTTGCTTTTTATTTCTTTATCTAAGAAATTATTTAAAATTTCTTTTACTATTCCTACATTTTTTGAAATTACATTTGCAACAAATTCATTAATGTTTTTAGATTCTATAACCTTATTATCTATTGCAGAAAGTTCAATAATTGATTTAATTTTTGGAAGTAGTTCGTTTTCTCATTGACTATCTATGTAATTTCTAGTTTTGGTGATTAAGGTTTGTAATAGTGAATTTAATTCAGCATCGTTAGATAGCTTGTTGTCTTTAAATGAATCTAAGTAATTTAATATAATAGATTTAATGTCAAATTTTTTAAAGACGTAGTTAACAAGTTCAGATTTAGAAGCAAGAACAGAAAGTGTTTTTATTAATTCAGAAAATTTTATTCCTTCTGATTTATTATCTTTTTCGAGTAGTAAATCAACAAAATTATTAATTAAATCCTTATTTTTTTCAATGTTGAAGTAGTCACTAAATATTGATTTTAAATAGTTTTTCAATTTATCAAATGTGTCAATTTTTTTATTATCTAAAATTTGAATTTTTTCATCAAAATTTTCAATAAGTTTAGTTACTAAATTGCTTTTAGTAATTGAATTGATTAATTTAATTAAATAATTGCTTATTTCTTTAATTTCTTTATCATTTAGTTCAGTATTTGTTTCTTTTTTTAATCAAATTGCGCCCAATTCAACTGCTTTATCAATAAATTTGGTGTTAGTTGCAATTTCTGATATCACCGTTTTTAATAAATCAATTGAACCATTCTTTTTAGAATTTTTTAAGAATGAAACCAAAAAATTATAGCTAGACGAGAAATCGTATTCATTATTTCCACTAATAAAATCATTTAAAATATTTTTTAAACAACCTAAAACTTTCTCACTTCTTATTGTTGAAATTAGTTGATCTCAAACATTTTCAACAAATTTTTGAATTTCTTCTTCATTGTTGAATATTTTTTTATAATTAATTGATTTAATGAAAGCAACGATAGGTTGAATAACAACATAATTTCCAATTTGATCAATTAATTGACTGAACAAACTATTATTCTTAAATAATTCATTTACTAGAAGCGAGATAATATCTTTAAATATTTCGCTACCAAATTCGTTTAAATTCTTCTTTGCATTTAATGCTATATTTTTATAAAATCTTAATATGTTTTCTCTTGAATCTTTATCAATTAATTTGCCTATGTTTTTTGGCATTAATGCATTATTTTTAATTGCATATTTTAGGGCTAAATGTTTTAATCCTTGTTTGTAGTCGTTAATATTTTGTATTTTTGGTTCAAACGATAGGATTTTAAATAAAAGCGAGTTATCCCTATTTAGGTCGTATTTATCAAAAAGAATTCCATTTAGAATTTCTGAAATTAAATCCCTATTTTGGTGGTCATCATCTAGTTTAGAATTCATTAATATTTGAATAATAATGTTTGATATTTGAGATAAACCATCGAAGTTTGAAATGTTGTCAAAAAATGTTGAAACAAACAAAGAAATATTTGGTTTTTCAAGTAAATCTAAATCATATGATTTGTATAAAGAATCAACAATTTTTCCGGTCAAATAGTTTGCAAGTTTGCTATCTTTTGAAATTTCTTTAATTATTAGGCTAACTAAATCATTTACATCTTTGCTTGAATCATGAACCAATGTAGTAATAAAGTCATCAATTTTTTCAAATTTAAAATATTTATCTGAATTATTAATTAACAAATTCAACACTTTTTGAAAAATGTTGTCTAAACCTTTTTCAATATCTTTGTTATTGAAAAGATTTTTGAATAAATCAACAGTAGTTTGAGGCAATAACCTCTTTAATATTTTTTTGATGAATACATCTGAAAATATAGCCTTAAATAATTTTGCATATTCACTTCTTTTAAGATCTTGACTTAAAGATTTGTTATAGAAAATTTCTTTTAAAATTTCATAAATATTTTGTTTAGAAGTAGTTGTTGAATAAACAATTTTTTTAATTTCTTCAAAGTTTAAACCTGCATATGATTTTTTATTAATAGCATTATTTAATGCGTTATTAATATCTAAAAACGATTTGCTTAAATTTTTTGAATCAATAAAAAGATTCAAAAAATTAATGATAGATTTTTTATTTTCTTTATTCTTTAAAGATTCTAATAAACACTTATTAAAACTGTTTAAATCTTTAGGGTCTAAGCCTATTAATCCGAATAGTATTGAAAATGTATTCAAAATTTCTTTATCTTCAATGTTTTTGTTTGAATTTAACATTGATTTTAATTGAAGGATTATATGGTTTGAATTGTATGAAGAATTAATATTTTTGATATCTGCATTTTCTTCAAATGTATATTCTTTTTCAAAAGAATCTACATTGTTGCCATTTATTCCTAAAATCATTGAAAAAATTTCAGATGGAGTTTTGCTAAATTCAAGTGATTGGCTAAATTCATTTAGATTAGAATTATTATCAACTAATTCTTTATATTTATTATTAGGCAATGACATTTTCATAAAAATATCTTGCGCTAATTTTTTGTATAAATTATTAGATGGGAAGCAGTCAAAGAAATTGTTGTTTAGTTTATCGGCATTATCTTTGAGATTTTTAATGTCATTATTAATGTTTAAATAGTTTAGCTTGTTTGCTTTGGCAATGTTTGCTAATGATTTGTTTAATTCTTCTATGCAATCATTAGTTAATGAAATATTGTATTGTTTGTCAATTAATTTTGTTAATTTCAAATAAGGCGATATATAACCCACAAGATTAATGTTTGCCTTAGGATTATATTGTTTAATGATTGAAATTAATTTTGAATATGAATCTTCAAAATTTTTACAATTTCTTTTAAATTCAAGAAGAATTTTTGAAAAATATTTCAAAATTTCTTCTTTATTTTTGTTTTCTCTTAAAAGATCAAACGCTTCTTTAAAAAATGATGATTGTTCAAAAAGGTCGTTGAATCCCAAAGAAATTGTAATCAGGTTTGCTTGTTTTATATTTTTAATTAGCTCTTGTAATTTTTTATTAGAAAAATCGCCAAATAGATTATTCAATTTGGTACTATTAAATTTTGAAAGTAAATTTTGATTATAGGAAATAACTTGATTTAAGTGAGCTGAATCCTTTTTGTCATCTAGCGAAAGTAATGAAATTCAATCATTAACATTTGAAAGTGGCAAACCGTAATTATAATAAGTTGATAATGTTGTTTGCTTATCAGATAATAGTTTTATAAAATTAGCAATGTATGAAGGATACGAAGTTCCTAAAACTAAATCGTTTTTGCTATCATATTCATTGTTGAAATAGTAATTTGAGCTGCTATTATAACCTGCAGCAAAATCATCGCCTAATGCAACATAATTAATTTTTTTAGAAACTTTATTTTCGCTTTGAGTTATTTTTGTATCCGGCTTCTTAGTTTTGTCTTTGCATGATATAGCAACTAATGAACTAGAAGCAATTGAAGATGTAATTCCTAAAGCAACAATCGCCTTGAGGATTTTTGTACTTTTTTTCATAAATACCTCCTAACCAATAGAAATATCTTCATAAATATATTGATATCTTTGTGGTTTTGATTTTGAAAAAGTAAAAATTTTTAGAAATTGTGATATTCCTAATTGTCCTACTATCATTGTTATAATTAAAAGTATTTTTCCAGTAATGCCCAAATTTGGTGTTAATCCAATATTAAGTCCTGAGTTTCCAAAAGCTGAGAAAACTTCAAACATTACATAGACCATTGGATATGACTCATTATAAACATTTTTTAAATCGCTTGGAAGTGTATGATATATTACTATTGTCATTATTAAAGTTATAAAAATTGCAATTGCAAAAATGTTAACTGCCTTAATTAAAGATTCTTTTCCTATTTGTCTATGAAAGGCATTTATGTTTTTGCGATTTTTAATTAAAGTAATTATACTCATAAAAATTATAGCCACTGTAATATTACGAATTCCTCCAGCAGTTGAAACAGGAGAAAATCCAATAAACATTAGGATACCGTGAACCATAATTGTAGGTTGAGTAAAATTATAATAATTTACAGTTGAAAAACCTGCTGATCTTGTTGACATAGTTTGAAAATATATTGCAAATACTTTGTCGCCTATCGAACCATATTCGCTTGAGTTCCAAAAATAATTTGCCCCCTTAGCAGTTGTTTCAAAGATAAATGTTAATAATAAACCAATTAGTGATGTAAAAATGTATGTAATAATTGTAAATTTTGTAAATAATGTAAAGCAATGACGAGGATTGTCTTTCTTGATGCTTTTAATTTTTTGTCAAACATCATAGAATACAGGGAATCCCATTCCACCAATTAGAAATATAATTGATGTTAAAAATAAAAAGTAATAATTATGATAATAAGGTTGCAAACTTTTTGTGCCAATTATATCTAATCCGGCATTATTTATTGCACTTAGTGTATGAAATATTCCAAATCTTAGTGACATACCAAAGTTATGATAAGGATTTCTTATTGATTCTTCATAAGGATTAAATGTTCATGGATTATTTTTACTTAATGCATTTAATTGAGACATTTTTTCATCACTAAAAAGTGCTGTATTATCATAATAAAATATTAAAGAAAATATTAAAGAAAATAATATAGAAATAATAATTAAAAAACTTATTGAAACTTTTATCATTTTTTTTGTTTCATAAATATTAGAACCGCCTCTTTCAGTTTGGTGAATTTGGCCACTAAATAAATTCGTTCTTAATCCAAGTAATGATTGAAAAATATAAAGTTTAAATGTAAAAATTCCAATTCCGCCAAGAATAAAACAGACAGCTATTAATATCTGTCCAAACATGTTAAATACTTCACTAACGGCAACTCCACTTAAACCGGTTGTTGTAAATGATGAGGCTGCTAATAGCAAAGCGTCAAGATAACTAATATGCTTTGAGGGATTATGACTAACTTCTCAATATAACATCAAAGACATTAGAATCGTAAAAAGTATATAAATCAAAAAGATAAATCTTATTGTTCCAAGTTTCTTTAAAAACACAAATAAGACATTTGATTTTTTTGGATTTCCCTTTTTAATCATAGAAAGAATTTTACCACAAAGTTAAAAAATGTTATTTTATAATACTTTACCATACCGCTTCTGTGTAAAAAAACTATAATATTTAAGCTAATATATTTAAAATTCAAAATTATTTTTTAAAATATTAAAATTTTGAAATGAAAGAGGTTATTATGAGTTTATTTAAAAAGATACGTGAAATTTGTATTATTGGTATAGGTCGTTATGGTTCGTCGGTAGCAAGAACATTATTGGCCGATAGAAATAATAATATAAGGATAGTTTTAGTTGATGAAGATGAAAAACATTTGTTACCTTTTAAAGATGAAGTAGAAATGATCTATGTTGCAGACTGTGCTGAACAAAAAACATTAGAAAGTCTTAATATAAAAGATTTTGATGCCGTTATTGTTGGCGCTTCAAATAATATTGAAATAGTTGCTGCTTTAACTGAATGTGGTGTAAAAACTATAATAGCAAGAGCTTCAACAAAAAGACATGCCCAAGTTTTAAAGCAAATTGGTGTTAGCTCAATTGTTTGCCCCGAAGAAGAAGCCGGAAAGAAAACTGCAATGTTAGTTGCAAACCCTGCGCTTGCATTATATTCAGAAAACATGGTTGAATTGCAAGACGGATTTGTTTCTGCTTCAATATATGTTAAAAACCCTGCTGTTGCAAACAAAAAAATTAGTGAATTAAATTTCAGAAATAAATACAATGTTTTAGTAACTATGATTAAGAGAGATAAACATTCATACATTCCTGAAGGTGATTTTGAAATAAAATTAGATGATTTAATAACTCTTATTGGCCAAATTGAAGATATTATTCAAGTTATTGAAATTTGTGCAATGAATGAAAAATAACTTAAAACAAGAACAAAAAAATAGCAAATTTTTTCTAGTTTGCTATTTTTAATTTTAATTTGTAAATATAGTAAAATTATAAATATTATCAATTTACAAAAGGAGATTAAATGGCTATTTTAGACAATGAGCTTCTTAAAAAATTAGAAGCAAAATACGAATCTAATTTAAATAACAAAATTATTGAAAATGCTATTTTTAAAAATGGTATTAAGGCTTCATCAACTAATAATCAAGTTGTAAGAAAACACGATTTTCAATTTTCTGTTGAAGTAAAACCTGGTTCAATAACTAACCAAAAACATTCAGGTAGATGTTGAATTTTTGCAGAACTAAATGCAGCAAGAGCAAAGGTTATGAAGGATTTAAACATTGAAGATTTAGAATTTTCACAAAATTTTATTCATTTTTATGACAAATTAGAAAAAGCAAATGCATATCTAACATGAATTGCAACAACTGGTTTGGAATTAGATAATGAAGATAGATTATTTAGAAGATATAATGAAAACCCTATTTCGGATGGTGGATATTTTGAATTCTTTTTAAATATTGCTAGCAAATATGGAATTGTTCCAAAATCTGCAATGCCAGAATCATTCCATAGTGAAGCAACAAACGATATGGTAGAACAAATTAATTGAAGACTTAAAGCATATACATTCAGAATGCGTGAAGCATATAAAAAGAATCATAATATGAGTGAAGTAGAAGTAATTCAACAACATGCTTTGGAAGATGTTTACAACATATTGGTTAAGTCCCTAGGTATGCCTCCAAAAACATTTATTTTTGAATACTATGATAAAGATAAAAAATTTGTAAGATTGCCAGAAATGACACCAATAAAGTTTTTTGAAACATACATTAAACAATTTGTTGAAAATAAGGTTGATGTAATTAGCGACCCAAGAGAAATTTACCCTTACTATAGACAATTGGTTGCTCCTTCGGTTTTCAATTCGGTTGGTGGTATTGAATATAAAATGTTAAATATACCAATGAATGAATTAAAGGCAGCAGCAATTAAACAATTGCAATCTGGTCAATCAATTTGATTTGGTTGCGACGTTACAACTTTTTATTCAAAAGAAGGAATTTTTGACACTAAATTATATGACTATGATTTAACATTAACAGAAACTCCAAAAGAATTTAATAAGAAGGAAAAATTCGAATCTCGTGCTTCAGTTATTAGCCATGCCATGAACTTAATTGGTGTTAATTTAGATGAAAAAGGGAACCCAATTAATTGAAAAGTTGAAAATAGTTGAGGAACTGAAGTTGGTAAAAAAGGCTATTGATCAATGAGTGATTCTTGATTTGAAGAATATGTTTACATGCTAATTGTTGACAAGGAATTTTTATCAAAAGAAGCAATTGAAGGTCTTTCAAAAGAAGCTATAGAAATTTCTCCATACGACCCATTATGTGAAGAATAATAAGTTTAAAAAAGTTGCACACCGCAACTTTTTTTATTAAAAATAATAATTTATATAATTATTAACTTAATACATTTATAATTAATACATTAATGTATTAGAAAGAGGAAAAATGTTAGGTGTGGACTTAACCGATATTAGACGATTTAAAGAATATAGTGAAACGCTTTTGATTAAGTTCTTACATCCGATTGAAATTGAACAATTTAAAAAGGTTTCGATAACAAAACGTGCTGATTTTCTTGCGACTAGATGAGCAATTAAAGAGGCAATCTTTAAAGCCGACAATCAATATTATTCATTCAACCAAATAAATATAACAAAAGATGAAGTTGGACGCTACGTTTTTAAAGATTTTGAAATTTCAACCTCTAATGAAGGAATATACATAATTGCAGTAGTTTTTAAAAGATAGAACAAATAGTAGGAGTTATTTATGAATTTAAGAACAAGAATATTTTTTAGATTTTTTGGTTTGTTACATTGCATATCGTTTTTATGTGCAAAATCAAGACGTGATTTTAAAATGCCTGATTATTACAAAGTAGTAGAAAAAAACGACTTTTTGCAACAAAAAAGTAATGCCATTTTAAACCATTTTAATATTTCTGTTAATGTTGAAGGGTTTGAGAATGTCCCTAATGGACCATGTTTAATTATTCCAAACCATTCAACGTATTTAGATCCACTAATAATGTGATCGGCATTATGAAACCATAGAGATGGTTCAAAACAAAGTAAATTGGTAAATTTCGTTGCAAGAAAAGAAATAACAAAAAAGAAAATGGTTAAAAGAATAGCAGATTTAGGTCAAACTTATTATATTGATACTCAAAATCCTAGAGAAGCATTGGCTACCTTGAAAGATTTCGGCCAATTTATTAAAAGGAATAAATCATGTGGCGTTATTTTTGCTGAAGGAACAAGAACAAAAGATGGAAAATTAGGCGAATTTCATAGTGGCGCCTTTAAACTTGCTCAATCTTCATATTTAACAATTGTTCCAGCAACAATAAATAATGCAGCAAATGCATTGGATTGAAATAGAAAAGAAAAATTAGAAGTTAAGGTGACATTTCATAAGCCAATGAAGCCAATTCAATTTCAATCACTTGATAGCCGTGTATTAGCAGGACAAGTTAAATCAATAGTTGCATCAGATTATATTGATCAAACAATTACATCAAATGAAACAATCAAAAACAAATATTCAAAACGAGACAATGTAAAAGAAGTTAAGAGATAAAATATGCCCGATTTAGAAAAACCAATTATTAGAGAAATTTCAATAAAAGAAGAACCAAACAAAGATCAAAACAATCAAACTAATTCATCAATTTTTAAAGAAAAACACGTATTTAAATTAAAAAATTTTGATGGTCCGCTTGATTTGTTGGTTTCTTTAATTAAAGAGAAAAATATAAGCATTTTTGATGTTGACTTATCTGAATTAGCAAATCAATATCTTGAAATAATTCATAGTCTTCAATCTTATGAAGTTGATATAGCAAGTGAATATCTAGTTATGGCTGCACATTTATTACAATTGAAGGCTAGAATGGTATTGCAAGATCCAGAAGTTGAAGAAGAAATTAGACAAGAAAAAAAGAGATTGCTCGAACAGATTGCTGAATATGAAAAATTCAAAGAAATTTCTTTACTTTTACGTGAACAAGAAGAAAAAAGATCTAGATTGTTTTCAAAAGAACCAGAAGACACACAAGATTTCGTAAGAGATATTGATGGTTCAATTCTTGATGGACATTCCAATAGTTCAAAATTAGTTGTAACTTTAAGAAAAATGTTTGAAAGAACATATGCTGAACTTATTAGAAATATTACTATTTCAACAATAGCAGTGAGTCCTGAAGAACAAAAAGAAAGAATTATTAAATTATTTAAAACAAAGTTGCAAGTGACCTTTAAAGATGTTTTCTCTGTTCCAAGTTTGGGACACTTTGTTATTACTTTATTGGCGGCATTGGATTTAGTAAGACAACAAATTATTGAAATTTATCAAGAAGAAAATGAAGGCGAAATTCATTTTAAGAAAGGGGCTTTATATGAAGAATAAAATTATTGAAGCTTTGCTATTTATTCAAGGCTCTGAAGGGTTAAGCAGTGAACAAGCACAACAAGTTTTCAAATTAAATACAATTCAAGAAGCAAGACGATTATTAAGGGATTTCAAAACATACTTTAATAACTTGGAAAGAGGAATAAAAGTATTGGAATTCAATGATGTATTCAAGTTTTTAACTATTGAAAGCGTCAAAGATTATATTAGTGATTTAGTAACTATTACTAGAAAACAAAGATTATCAAATGCCGCAATTGAAGTTGCTGGTATTGTTGCTTATAAACAACCTATAACAAGAGGCATGATTTCAAACATAAGAGGTGTTGTAAGTGATGGAATTGTAAGTAGTTTATTAGAAAAAGGAATTATTGAAGAGGTTGGAGTTGCTCAAACTCCAGGCAACCCTATTTTATATGGAATTACTAATAAATTCTATGATTATTTCAAAATAAAGACCTTAGGAGAATTACCTCCTTTTCCTGAATTTAATAAATATAGTAGTGAAGAAGAGGAAGAAATTGATAAAGACTTCAATTTGTTTGATTCTCAAAGACAAGAAACAGACAATGATGGAGTTTTAATTTCTAATGAAGGGGCAGAATATGATAAATTTTAAGGCTAAAAAGGATGACGAAGGTCGTATCCTTTTTAAATATTTGGTAAAGGTTTGCGACAATGTTCCAATATCTAGAATAGAGCGACTTTTTAGAGAAAAGGATATCAAGGTAAATAATATACGTGTAAATGATAAGCAATACAAAGTCAAAGAAGGAGATTTAATTTCAATTTATGGATTAATTGAAACCTCCAAACATATTGACAATAAAGCTAATACAAACATAAATTTTAAAGTTATTTACGAAGATAAAAATCTTTTAATTATTGACAAGGCACCAAATATTGCAATGCATTCTGAAGAAAATTGTCTAGATGATCAAGTTTTAAAATATCTAAATTTTAAAAAAGTTTCTTCGTTTAGACCTGCTTCGATTGGTAGGATAGATAAAAAAACTAGCGGTTTGGTTGTTTATGCAAAAAACTACAAAACTCTAGTTGAATTTGAAAATAAACAAAATAAATTTGACAAAATTTACCAATTTGTAAGTGATTTTGATGAAGATATTTTGGATATAACAGTAAGACTAAAAAAAGATATTAAAAACGAAAAAATGATTGTCGATAATAATTTTGGCGTTAAAGCACAGACAATATTTTATAAAGAAGGAAATAGAAAATTTGCCAAGATAATAACAGGCAAAAAACATCAAATTAGGGCGACATTAGCATATTTAAAAACACCTATTTTAGGAGATATTAAATACGGCGGAAAATTTGCTAAAAGAATGTATTTGCATTCATATTCAATAAAATTTCATGATCTTTCTGATGATTTTAAAGAATATAATGAACAAGTATTCATTTGCTATCCAAAATGATAAAAACGATTAAGGAGTTAACATGGAAAAAGAAGAGATTAAAAAATTGGCTAATGATCTTTTATTTGAACCAAGTGAAGAAGTTATTAAATTAACTAATAACTTGCTTAATTCAATAGAAAAAGGTTTAAAAGATTTAGAGGAATTTGACTTACAAGACATTAAACCGATGTCGCACATCAATGAATCACCCATTTGTTTCGAACAACTAAGAAGTGATGAACCGGATAAAAGTTTTTATTTGAATAAACAAGATTTATTAAATAATGCATCTGAACATGATGAAAATTACGTAATTATGAAGAAGGTAATAAATGATAAATAAAGGTAATTTAAAAGAAGCAATTTTACAATTAAATGCTGATAAAAATAATGCGGTATCATTTGTTTTTAAAGATGCTCAATTAAAAGAACAAGGACCGCTTGCAAATTGTGTTTTTACAATTAAAGATAATTATGCGGATTTGAATGTTATTGAAAGCGCTTCAAGCAGATTTCTTTCTAATTTTAGACCTTCATATCAATCTACAATCTTAAAATTATTAAATGAAGCAGGAGCAACGTGCGTTGCAAGAACAAATTTGGACGAATTTGGTATGGGCGGTAGTGGAGAATATTCATGCAATGGATTAATAAAAAATCCTTTGAATAGTAATTATTTAGTTGGGGGTTCTTCATCGGGTGCCGCTGCGACTTTTTCTAAAAATATTTCGTTTGCAATTGGATCGGACACAGGCGATTCAGTTAGAAAACCTGCATCAAATATTGGCGAGGTTGGATTTAAACCAAGTTATGGTGCTATAAGCCGTTATGGATTGTCCGCTTTTGCTTCGTCATTAGATACAGTTGCATATTTTACACATAATGTTCAAGATGCAATAACTCTCTCATCAGTATTGTTTAAGCAAGATTTAAAACATGATTTGACATCAATTGATTTGTCGTTTAATGCAGATAGTGTTATTGAAACAAAACCACATAAAATTGCTTATTTGGATTGTTTTGACAAATTGGATATATCTGTTGCTGATGCATATAAAAAATTATTGAATAAGCTAGAAAAAATGAATATTGAACTTGTAAAAATTTCAATAAATGAAAAGTTACTGCAAGCCATCGATACTGTTTATAAGGTTATTGCTTTTTCAGAATCATCATCTAATTTAGCAAATCTTGTAGGTGTTGAATTTGGCAATAGAATTACTGGCAATTCTTGAGAAGAAACATTTAATTTATCAAGGACAGAAGGTATTGGAAAAATGGTTCAATCAAGAATGATTTTAGGATCTTATTTTTTAGAAGATAATAATCAAATAAAGTATTTTGTTAAGGCTAAAAAAATGAGAAGGTATTTGAGCAATTATTTCACTAATGTTCATAAAAGTGCAGATATATTTATCTATCCAGCATCTAACATGTCCGCTCCTCTTATTGGAAAATTATATGAACCAAGTTATATGGATTATATTTTAACAAATTCTAATTTGACAGGAAATCCTTCTTTGACATTAAAATTAGGAATTGACAAAAATAATAATCTTCCATTTAATTTGGCAATTGACGGAGAAATATATAAAGATTGCAAGATGCTAAGTCACGCATTATTTATTGAAAGAGTATTGGAAGGTGAAAAAAATGAATAATGATTGAGAAATAGTTATAGGAATTGAAATTCATATTGAGTTAAATACAAAGACAAAAATGTTTTCTCCAGTTCAAAATGAATACGGAGATGAAGCCAATACAAATGTTTCAAATATTGACCTTGCCTATCCCGGAAGTTTGCCTCTTTTAAATTCTAGTGCCATAATTAAGGCAATTAAATTAGCCAAGGCGCTAGAAATGGAAATAGACCCATTAGTTAGATTTGATAGGAAAAATTATTTTTATCCCGATTTAACAAAGGGATATCAAATTACTCAACAATTTCATCCAATTGGTAAAAATGGTTTGATAAGGGCAAAGGTTCAAAATTCATGAAAAACTTTTTCTATTGAAAGAATTCATATGGAAGAAGATACTGCTAAATCAATTCACGAAAATGGTTTGACATATTTAAACTATAATAGATGTGGAGTTCCTTTAATTGAAATAGTTTCTAATCCTGTAATGCACAGTGCAGAGGATGCAATGGCTTATGTTGAAGCCATAAGACAAACAGCATTAGTTTTAGACATTAGTGATGCAAAATTAAATGAAGGAAGCTTAAGAACTGACGTTAATTTATCAGTAAGAAGAAGAGGAGAAAATATTCTTAATACTCGCACGGAAATAAAAAATTTAAATTCTCTTTCAAATATTAAAAAAGCAATTGAATATGAATCTAATTTCCAAATTCAAATGTATGAAAACAATTTAACTTTTAATCAAGAAACAAAGCGTTTTGATGAAAAAATGCAAAAGACTGTTTCAATGCGTTCAAAAGGTGATGCAATTAGTTATAAATATTTTCCAGATCCAAATTTACCTTATATTCAATTGCAACAAAAATTTATAGATGATATTAAAATAGAAGAAATACCATATAAAAAAGAATTAAGATATCTAAAAAATGGCTTAAATCAAGTTCAAATATCTCAATTGTTGAATAATATTTCTTATGCAAAATATATTGATAGTATAAACTCAAAAGATTTTAAAAAAGCCACAAATGTTTTCTTTTCTGACATAGTTACCTATTTAAATAGTAACAATAAGAATATTGAAAATTTATCATTTAAAGCAGATGATGCAAGAAGGGTTGTCGAATATTTAATTGATAATAGGATTAATAAAAATAATATTGCTGAAATTATCAAAATTAAAGATCAAAATCCAAATTTAAATATTGAATCTATTATTAAAGACAATGGCTTTTTAATTGAAATAAAAGAAATTGATTTGAACGAACTAGTTCAAGAAATATTAAATGAAAATCCAAACTTGGAAACTGAATTTAAAAACAATTATAACCGTGCCCAAAAATTTATGCAAGGTCAAATAATGAAAAAAACAATGGGAAAGGCAAATCTAAAAAATCTTGATGAAATAATCAAAAATAGGTTCAATAATGAGTAAAAAATGAAATCCAAATAAGCTACCTACGGGTTGAAAAGTTGATGAAGATCAAATAAATAGAAAACCAACAACTATTTCTTCAACCCAAATTATTGCAAGAAAAAAACGAAACAATATTTTAGAAAAAATTCTTAAGTGAATTATTTATGGAATTATTTTTATAGCAATACCTAAATTTATTAAAAGTTCAAAGACAAAAGGAAAAGAAATTGTAAATTCTGCATATGAAAAAATAAATTCAAATGAATTTTCTTTTGTCCCCGCTGGATATGCAATGTATCTATTTTTGTCTTTTATTCCAATCTCAATATTGATGCTTGGAATTATTGGCTCGGTTGATGAAAAGTATGAAGTGGTGCTAAAGAACGTAATTTTGGGTCAAATTATTCCCGGAATTTCAGAAGTAATTCCATCATTTAGTACATTATGAGGTTCGACAGGCAATGCAATTGCTTTAATCTTATTTGCATTATCAGTACTAATTTTAGCATCGAAGGGATATTCAAAATTTATTTTTTCAATAGATGCTTTATATGAACACAAATCTCCATTCAGAGCGTGAAAATCGAGAATTAAAGGATTCATAGTAAGTGTCTTTGTCTGCTTGATATTGTCTTTAATCTTACTAGGGCTAACAGCCTTTATGGTATTTTTAATTGATAAAGTAGGTCTTGGAAAATTTGATGTCGATATTAAAAAAATGAAATTAGCAGATTTTAATTTAACTACAGAATTTTATTTAATATATTGATTTACAACAATTTTGTTTTTACCTGTATTTACTTATTTAGGATTTCTATTTTATTTTGTATATGCCCCAAATTTTAAATTGAAAATGTCGCAAGCACATCCTGGGGCTTTGATAGCAGCAATACCAACAGCAATATTTATTTTGATTTTTGGTTCATTAACATCCTTAATTCCTTATAAAAAATTTGGGATAGTTGCTTCATTTATGTATATTATTTTATTATTGAGTTTTACTTCTTATTTTATTTACATGGGAATTATTGTTAATTCAAGTTTTTATAAAACTTTTATTAATTTACCAACTGTAGAAAAACATTCATTTTGAAAAAAACAAAAAATCTCAATTTAAAGTCATTTGATTTTTTCGCTAATTTTTCATTATTTATAGTTATTAACGACATTTAAAATATAATTATTATAATAATTAATAAAGCAAAGGAAATATATGAATAAATTACCCGGAGGGTTAAGACACTATATTTATTGAGCTCGGTCACAGACAAATATTTCATATCGTAAATGAAACTCAAAACGTATTGCGTTTGTGGGTGTTTTGATTGCTATCTCAGTTGTCTTTTTTCTAATAAGTGTTAGAATCATGCCTATAAGTGCCTTACCAAGTATAAAATTTAGCTTCATTGGTTTGCCAATAAAAATAACTGGTTTTATTTTTGGACCAATTGTTGGTATGATAACTGGGATATTGGCGGATATAATTTCATTTGCTCTAGTTCCAACTTACTATAATGTTTTGTATACAATAGCGGTTGCGGTATCAGGGTTTGTTCCCGGGTTTGCTGCGTACTATTTTTTCAATATTAATGAATTGTTTTTTTCAAGAACGTATAGAATTTTTAAATACAAAGAAACAATTGATTATTTTAAAGAACAATATGATATTGCACTTACAATAGGCAACTCCGAAGATATGCAATATTTTTCAGAAAAAATTGCTTTTTATGAAGTCAAGATCATAATTTTAGAATGTAAGAAAAAGCCAATTGCCATGATTAATTTTTCATTTATTTCAACATTGATAATATTAGGTATTCAAGTGTTGATAATTCTTGCAATTTTTTCTAACCTAGATCCTTCAATATTTGAAAAAAATAGATTTGTCAAAAACAAAAAATTCTATATGTTCTTAACTTCTATAGGATTTATAGGAATGATGATTTTTGTCTCTCTATATAGAATATTTTTAAAGAAAAAATTTGAAACCTTTTTAGAAATAATGGCAATAATTTCATTTAGCGCTGTTTTAGAAATAGTAAATGTTATCTTATTGGCGTGAGCAGATACTTCTACATTAAAGACAGATTTCTGGGTTAATTTTACTGCTCAAAGTCTCTTTAGTCCCGTTAAAATATTTTTTAATTTAGCAATAATATTAGCAACATATAAAGTTGTTGCACCTTTAATTAAATCAAAAGAAGGTGGACATTTATAATGAAAAAAATAGCAATAAGTTCTTTAAAAGTTACTAATTTAAAAAGTATTAATGTTGTAAATGAACAATTTAAAAACTTAATTAATAATCTATCTTTTACGCTTAATGCAAACAATATATTGGGTTTTGTAGGTGGAGATAATGAAACAAATAATTTAATATTTAATTATTTAACTAATAATACAATAAATCCTAAAAGATATCAAGGTTACATTGAATTTCAAACAAACTTAGGTGAATATTCTTCAATATATAAGAACTCAAATTATCAAAATAACATAGCTTTTGGTTTTTATAATGATATTTTAAACAATGATTTAAATGAAGATAATAGCTATAGAAATATAAAAAAATATATTAACCACGGAGCAGTTATAGGGGCACTAACGGAAGAATTTGTTTCTCAGTGAAAGCCAGTTTTTCAAGACTATAAATTATTGCTTCAAAGCGAATTTGCAAAGTTACAATATGGTGTTGAAATTGAACTCGGAAGCAAATTAGATGACTTATTCAGTGAATTAAGTCAATTTGATTTGCGAAATATCGACGCTAAAAATTTAAAATTTGATACTCTTTTTAAGAAATTTAAAGATATAGAAATTTTATATAGAACCCATGAAATGAAACTATTCGCATTTGTTAATGCGACTATAGCAAAATATAATAAGGGGTTATCGTTTTTAAAACGCAAGGAATATTTAGACAGTAAAAAGGAATACGAAGAGGTAATAAAAAAAATTTATAAACCTTTTTCAAAGCCTAATAGATTTTTAAATCGAGTAAGATTTTTTTCTAAATTTTTAGAAACAAAAAAATCTTCAAAATCATCTATTAAAAAATTTGTAAATAAATTAAGGCGTGATTTAAATATTGAAAACTATTATTACAAATATGAATTAAAGAAAACAATAAAACAATCTTCGTTTATTCACTTTTATAAAAATCTATATTTTAATTGTCTATTATTGAAATTTATAAATAAAAATTTAAAGGCCATATTAGGGCTAAAAGAAGCAAAATCTTTTGAATTATTGTATGAAATTTTTGATTTAAAAAACAATATTACAAATGAAGTTAATGTAATTTCTAACATTGATAATCAAAAAGAATTAAAGAATGAAATAAAGGCTGTAATAAAAAATAATTTTTTAAATGATGTGATGCCATATATTGAAAGAATTAAGGCGTCTAGTTATGAACACACTCAAGAAGTTATTAATGATATTACAAGAGAAATTCAAACGTCTAATAACTATGATTTAAACATAGGCGAGTTTGGCCTTGCAAACAAAGCGTTTGAAAAATTAAAAACGGTTGAATCAAAATATAAAGATGCAAAAAATGAATATTTTTGAAATTATAAAACTGAAGGGAATGCCATTTTAAAGGAAACTAAAAAAGTTTTAAGAAATTTTAATAAATATTCAAAAATAAACTCACATAAATTAATGAAATTAAAACTAAAAATTTTTAATTTGATTAATTTAATAAAAATTAAGGAATTTAATAATTCAGTTCTAAATGAGCAAGTAAAAAAAATAAAAGAAAATCTGCAATTTTTAAAAATTTTAAGAAATGAATTTGATTCTTATAAATATGTTGTAAGTGATTTTAAAACGGCATCAAAAAAACAAATAAAACTTTTTGTTTCAAAAATTAATATTTACAAAATTATTGCAAAGGCAAATATTGACGTTAATTTTTTAGTAGAGAAATTAGGCTCTTTAAGCGATGATGAAAAAATTAGATTAGAGTGCGAAAAAATTTTAATAAATAATCCTTCACTAATAATAATAGGACCCAATATTTCTAAATTACCATATGAAAAGCAATGCGAAACATTGAATAAATTGAACAAATTCGTTTTAGACAATCACAAAATGGCAATTTATTTCTTAGACAATATAAATATTGCTCAAAAAATAACTACAGATTTATATATTATAAATTCATCAAAAATTATTGAGCAAGGAAAAACTAGTCAATTAATAAGGAACCCAATAAATCCTATAGTCAAAAATATGTTAAATTACAAAGACGAGAAGACTCAAGAGGATTTAAGCTTTTATATGTCTCAAGAAGACAATATGGAAAATATATACAAATATGAAATAGAGCCATACCACTTTGTTTGATGCACATGAAGCGAATTGAACAGATGAATAAACGCAAATAATGTTAAAAACAAAGAGCTAATGAGTTTATTTGTTCCACAAAATACTTTTCAAAGTAAAGGAAATAAAACAATAGACAATAAGGAACCATTTGAAGAAACAACTCTTATAAATTTAATCGATGACGAAGATAACGAAGTAAGGAATTTGAACATGGAAAAAACTTTTAATCATAAAATAGTTGAAAAAGATAGATGCAAAAAATGAAATGATATGAAGATTTTTCAAACTCACGATGAATCTAAAAAACCATTTACCATTATTTTACCACCCCCAAATGTAACTGGCAATCTTCACATTGGGCACGCCCTAGACAATTATATTTCTGATACAATAATAAGATTTAAAAAGTTACAGGGATATGATGTATTGTGACTACCAGGAACAGATCATGCAGGTATAGCAACTCAAGCAGTAGTTGAAAAGAAACTAGCTTCCCAAAATATCAATAAATATCAATTAGGAAGAGAAGAATTTATTAAAAAGGTTTGAGAATGAGTAGATGAATATTCAAAAAATATTCATAACCAATGACAAAAATTAGGTTTGGCCCTTGATTATGGTTTTGAAAGATTCACTTTAGATGATAATTCTAATGAAGCTGTATTAAAGGTATTTATTGAACTTTATGAAAATGGTTTAATATATCGTGACAAGAAAGCAATAAATTGAGATCCTGTATTAGAAACAGCTCTTTCTAATATAGAAGTAGTACCAAAAGAAACAAAGCAAAAGATGTACTACATAAAATATCCAATTAAGAATTCAGAAAACTTTCTTGAAGTAGCCACAACGAGACCCGAAACAATGTTCTCAGATGTAGCTTTAGCAATAAACCCGCAAGATAGTAGAGCAAAACAATTAATAAAAGAAATAATAATACATCCATTAACAAATAAAGAAATTCCAATTATTTTATCAAACAAAATAGATCCTAATTTTGGTAGTGGAATAATGAAAGTTTCAGCCCATGCTGTTGATGATATTGAAATTATTATTGAAAATAAACTTCCAATAAATGAATGTATCAATAAAGATGGGAAATTAAATGATTTAGCAGGTGAATTTAAGGGATTAGATCGTTTTGAAGCTAGAGAAAAAATTGCACACTTATTAAGTCAAAAAGGACTTTTAATAAAAGAAGAAGAAGTAGTTTCTAATATTGGATATAGTGAAAGATCAAAGGCCCCAATTGAAATTTTAGTTCAGCCACAATGATTTGTTAAGATGTCAGAATTTTCAAAGATGCTTTTAAAAGATTTGTGCAGTGACGAAGGAGTAAAAATTTTTCCACCACGTTTTGAAAGCACGCTAGAAAATTGAATGGAAAATGCTCACGATTGAACAATTTCAAGACAAATTTGGTGAGGGCATCGGATTCCCGCTTGGTACAATGAACAAAATGAAATTAAAGTTCAAATTCAAAAGCCTGATGGAAATTGAAAACAAGATGATGATGTTTTAGATACTTGATTTAGTTCGGGACTTGCACCATTTGTATTTTTAGGATGGCCACAAGACAAGAGTAAATTAAAACGTTATTTTCCTACAAGCTTATTAGTAACTGGATATGATATTATTTTCTTTTGAGTAGCAAGAATGTATTTCTTTAGCTTGTATTTTATGGATCAAAAACCTTTTGAACATCTCTTATTGCATGGATTAGTTAGAGATTCGCAAGGAAGAAAAATGTCTAAATCGCTTGGCAATGGAATTAATCCAATTCAAGTAATTGATGAATACGGTTCTGATGTTTTAAGAATGTCTTTAATTTTTAATTGCACACCTGGCCTTGATATTAATTACAGTGATGAAAAAATCCAAGGTGCTAAACTATTTGCTAATAAATTCTGAAATATAGCTAGATTTGTTGAAAATATTCCTGTTTCGATAACTGAAAAGATTGATTTTAATAAGTTGGATGAATATGATAATTGAATTTTAAATGAATATTTAACATTCAAAAAAAATATTGAAGATGCCATGGATAAATATGAGTTAACTATTATTTATAAGCATATTCAAGATTTTGTTGTTGACAAATTCTCAAATTGATATTTAGAATTTTTAAAATTTAAAGAAAATTCATATTTAATACATTATTTATTCAAACAAATATTGATTTTATTGCATCCATTTATGCCGTTTCTAACAGATTATGTTTTTGAAAAATTATATCATGAAGAATTGCTATGCAGCAATCTTCAAACTTTTGCTTTAAATAAAGAAAGTTCAAACAATACTCCTAAAATTATTTCTTTAATTACTGCTTTAAGAAAATACAGGGAAGATAAAAAGATTTCAAAATCTATTGAATTATTCTTCTATTTGAATAATCTTGTATTCAGCAGTATAGATTTAAAAATAATTAACAAATTAAGCAATTTTTCTTGAAAAGAAAATCACGATTTTTCACTAAAAACAGAAAGTTTTGAAGTATTTATAATTCAAAGTAAGCAAGACAAAGAAAATGAAATTCTAGAATTAAATAAATTAATTGAATTTAGTCAAAAAGAAATTGAATTTAATGAAAAATTAATTAATAATCCTAAATTTATGGAAAAGGCTAATAAAAATACAATCAATGAAAAATTAGCAAATTTAAAATTGCATAAAGAAAAATTAGAGTTTTATAAAAACCAACTTAAAGAAAAACAAGAAAAAATAAATTAATTAAAAACAAGATAAGAGTTTTTTAATAAACAATTATCTTGTTTTATTTTTTACTGTTATAAAATTGCATTAAAAATTATTATTTGTCTTGAATTGCTTCTATTCCAGGCAATTTCTTTCCTTGGACAAATTCTATTGATGCTCCACCGCCAGTTGAAATAAAACTAAATTTATCTTGATAACCTAATTTGTTTATTGCTGTAACAGAGTCACCCCCGCCAACTACTGAATAGCAATCTCTATTTTGAGCAATAGCGGTTGCAATTCCTTTTGTTCCAGCTTCAAAGTTACTGAATTCAGTCACTCCTGCTGGGCCATTTCAAAAGATAGTTTTAGCATTTTTAATAATACTTGCAAATAATTTAATACTTTCTGGTCCAATATCTAATCCCATATTTCCGTCAGGAATATCAGGATTTTCTGGACTTGTAAATTTAGGAGTTTTGTCAGCGAATTCATCAACATAAGCATTGTCAATTGGCAAAACAATTTTATCTTTATATTTGTTTAAATATTCTTTTGCCATTTCAACTTTATCTTCTTCAAAAATAGAAGTTCCAATTTTTCTATTTAAGGCTTTTTGGAATGTATATGCCATACCACCAACAATTAAAACCTTATCTGCTAGTTCAAACAACTTTTCTAGAACTTTGATTTTATCGCTTACTTTTGCTCCACCAATTATTGCAACAAAAGGACGTTTAAATCCTACTAATAATTTTTGAAGGTGTTCAACTTCATTATTCATTAAAAATCCTAATGCTGATTCTTTAGAATATTGTGAAATTCCATATGTTGATGCATGAGCTCTGTGTGCAGTTCCAAAAGCATCATTAACATACACGTCGCCTAGACTTGCTCAATATTTTGCAAGATCTTCATCATTATTAGATTCTGCTTTATTATTTAAATCTTGAAAACGAGTATTTTCTAAAACCAAAATTTCTCCAGGTTTTAAGTTTTTAGCAGCATTTGTAACTGCTTCACTTCTATTTTCGTTAATAAATTTAACGTCTCTATTTAATAATTGCGCCAATTTTTTAGCTACAATTTCTAAAGATTTAGTTTTCTTGTCTTCTTCTGTTTTAACTCTTCCTAAATGAGATAGAATGATTAAAGAAGCATTTTTACTCAATATGTATTTGATTGTTGGCAATTCTTCATCAATTCTTTTTGTGTCCATAATAGTATTGTTTGAAATAGGAACGTTAAAATCAACTCTTAAAATAACCTTTTTATTTTCTAATTGAAGGTCTTCAATGGTTTTCTTCATTTTATCTCCTTGTGTGTCTTGTATGTTTTATATAAATAAAAATAATATTTTGTTTAAATTTATATAATTATAAAGAAAAAAAGTTATTTTAAAATCAACTTGGGAGATTAAATGGCAAAAATTTTAAATGCTCAAAAACAAAAGGACACAAGAACTCTTAGCTATGATCCTGAAAAAGACAGCTTGACTTTGCTAATTTCAGAGTTTCAACTTTACAATAAAAACACCAAATTTATTAATGATTATTCAGAAATGGAAAAATTTCATTATTTTAAAGAATCTAGATGAATGAAAACAATGGATGAAAAATTGCAAGATCAAGTGCAAGAATTTTCAAAAAGGCAAAAAAAATTAAATGCCAAATATAAAAGTTACATTGAATATTCTAAATGGTCTGAATCGCCATATGCAACAAAAACTAAACCGCTTACAAAATGAAACAGAATCATTATTATTTCGGTTTTCATTATTTTGGTAATTATCATGTTGCTAATCGTAATTGGCTTGAATAAATTGTGATAAAAAACGGAGGTATTGACATGGAAATAAAACTATTAATTGTTTTACTAGTGTTGCTAGCAGGTGTTATTATTTTCATAATTGCTAATTATTCAATTACTTGAATTAAAAACAAAAAAAATTCAAAAAGTAACAAAAAGGTTTTGGGTGAATCTACAAAAATAAGAATTATTTATCAATTAAAAGAAGCCCTTGAATTTTTATCAAAGACAAAGACTGGTGCAATTATCACCATCGAAAATAAAGATAATCTTGATTTGCTAAGAACTGACGGCGTGATATTAAATGCTAACATTTCTGCTTCTCTAATTATTTCTATTTTTAATAAACACGCTCCACTTCACGATGGCGCAATAATTATTAGAAATAACAAAATTTATTATGCTGCCACATATTATAAAATAACAAAAAAATCAATTGATAATAAATATGGCGCAAGACACCGCGCTGCTATGGGTATTTCAGAAATTTGTGATGCTACAACTATAGTTGTTAGTGAAGAAAATGGTGGAATTACAATAGCTAAAAATGGAACTTTTAGCCCTGTTTTACTAGAAAATCTACAAGAAAGATTAGTCAATATTTTTAAGGATAATAATTAAAAAAATAAAAATTTTGAAATTATTAAAAAAGTATGGTAAAATGTATTTCGCAATTTGAAAAAAGCGATCGTTTAATTGCTCAAATTGCCGCTTAAAAAAATAAATATTATAGTTATATAATATACTCTCGCTCTTTTCTTATATTTATAATAAACCCTTATTTATTTTATACACATATACATACAACCCTTATTTTTTATATTTCCTTAACTTAGAACTTGAAGTTCAACTTATTATTAAATTAAATAATCAAAAGCTTATATTGTTCGATTTTTAGCGAGAGTAATCTTATTTTAAAAAATATTTCTTTCAAAATAGTAAACAAAAATGTTTGCTATTTTTTAATATTTTTGTTTAAAATATATAAGTACATAAATATCACTATTTTATGGCCAAAATTAAATCATTTACATTAAAAAAATATTTTTTTTGTTAAAATTACATTGCTATAAATGCGCGCTTTTGGACAGGACATAGTTGAAAGGCTATGCTAAGGTAGGTTAAACGCGTTTAATCAAAAGATTTAATAAATATTAAAAAAATTAAAATGTAATTTATTGAAAGGAAATTATTATGGCAAAATTAGATTTTGACCGTTCAAAACCACACGTTAATATTGGTACTATCGGTCACGTTGACCACGGTAAAACTACATTAACTGCTGCTATTGCAACAGTATTAGCTAAAAAAGGTCTAGCAGAAGCTAGAGACTACGCTTCTATTGACAATGCACCAGAAGAAAAAGCACGTGGTATTACAATTAACACATCACACATTGAATATCAAACAGAAAAACGTCACTACGCACACGTTGACTGTCCAGGACACGCTGACTATGTTAAAAACATGATTACCGGTGCTGCCCAAATGGATGGAGCAATTTTAGTTGTTGCTGCTACTGATGGTCCTATGCCTCAAACTCGTGAACACATTTTACTTGCAAGACAAGTTGGTGTTCCAAAGATAGTTGTATTTTTAAACAAAATTGATATGTTTAAAGATGATGAAAGAGAAGAAATGGTTGGACTAGTAGAAATGGACGTAAGAAGCCTTCTATCAGAATATGGTTTTGATGGCGACAATGCTCCTATTATTGCTGGTTCAGCTCTTAAAGCATTACAAGGAGATCCAGAATATGAAAAAGGTATTTTGGAATTAATGGATGCTGTTGATACATACATCGAAGAACCAAAACGTGAAACAGACAAACCATTCTTAATGGCTGTAGAAGACGTATTTACAATTACTGGTCGTGGAACTGTTGCAACTGGTAGAGTTGAACGTGGTGTTTTACAATTAAACGAAGAAGTTGAAATTGTTGGATTAAAACCAACCAAGAAAACAGTTGTTACTGGTATTGAAATGTTCCGTAAGAACTTAAAAGAAGCACAAGCTGGAGATAACGCTGGATTATTGCTACGTGGAATTGACAGAAGCGAAGTTGAACGTGGACAAGTTTTAGCAAAACCTAAGACAATCGTTCCTCACACACAATTCGAAGCAACTGTTTATGTTCTTAAAAAAGAAGAAGGTGGACGTCACACACCATTCTTCCACAACTATAAACCACAATTCTACTTCCGTACAACAGACGTTACTGGTGGAATTGAATTCAAACCTGGACGTGAAATGGTTGTTCCTGGCGACAACGTAGAATTAACTGTTACTTTAATTGCTCCTATTGCTATTGAAGAAGGAACCAAGTTCTCTATTCGTGAAGGTGGAAGAACTGTTGGTGCCGGATCAGTTACAAAAATTCTTAAATAATTATCAAAATTATTAAAAGAAATTTTGAATTACAAAATTGGATTTGTATAAATCAAGTCCAATTTTTTTATATTTTATTTAGTATGTTTAAATTGTAAATATTGCCGTTTTTATCTTCTATTATTAATTGTTAATAAAAAAAGTAACCATTTGTTCTTTAAAAAATGCCCCCCCCCCCCTATTTTTTAATTATTTTATTAACTTTTTAGTTAATAAAAATAAAATTAATTAATTAAAAATATAACTAAATTTCCATTTTTAATATAGGAGAACGAATGAATGAAAAAAAGAAAAAAATTGCCATTCCATTGGCAATTTTATGTGGCGGTTTAGCAATTGCTACAACAGCGCTTATTGCAATAAAGGCTCGCCGTCATAAAATTGCTAATCAATTGCAAAAAGAAAATTTATTGCAAAATTTTAAAAAATTACAAAAGCAATTAAATGAATTGCTAGGATATAAAATTGTTAATGAAATAAATGTTTTTCATGAACAAGAAGTTTTGCAAGGATCACTAAAAATTAATAATAAATCTGAAACCAAAGTAATTGAAGAAGAAACTTTAAGGTTAAAAGATGCTATTACTTTATTAATATCAAAAATAAAAAATCAAATAAATCAAAAAGAATTGGAATTTGCTAAATTTAATGAAATAAAAGATAAATTGCAAGAATATATCAAAAACGAGTTATCGAAACAAGAATACGAACATATTAAACAAAATATCGAAAATGAATTAAATAAATATACTCCAATTAGTCTTGAATCAACTTTAATCGAAATACAAAATGCAGCAAATAATCTAATTAAATTGTTAAATGAATCAACCAAAGAAAAAGATAACATTGATAATTTAAATGCCAAAGAACAATTAAAAGCATCTATTTCTCAAGCAAACCAATTATTGCCTCAATTAAGTGACAATGATAGTGAAATTGCAAAAGCTAAGAAATCATTAGATGCTGAAATTAAAAATGCAAATCAAGCAGTTGCTTCAAACAATACTGCTTCAATGCAATCAGCTAAGACTACATTAGATGCTAAAATTGCCCAAGTAAATCAACAACTACAACAATTTAATAAAGAAAAAGAAAACAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTTATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTGATGCTAAAAAAGCAAAGGAATTAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCATTGCAAACAGCTCAATCTGCTAAAACTGAAGCTGATGGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTTAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGATATTATTGCAGCAAATGATGAATTAAAACAAGCACTACAAACAGCTCAATCTGCTAAAACTGAAGCTGATAGAACAAATGGCGATGCTAAAGCTAAATTATCTACAAGTTTAACTACTGCAAAAGAATTAGTTAAAAAACTAGTTGATAGTGATGGCAAAATTCAACAAGCAAAAACACAATTAAATCAAGAAATTAAAAAAGTAGAAAGTGCTATTGCTTCAAACAATACAGCTGCAATCCAAGCATTGCAAAATCCATTTGATACAAAGATTTCTGAAATTCAAAATCAATTAACTGAGTTTAATAAAGATAAAACAAATAAATTCAATGAATTAAAACAAACAAGAAGTCAAATTGAAGCATTCATTAATGCAAATAAAACTAATCAAAATTATGCAGATTTGATTGCAAAATTAACTAATGCTAAAAAAGCAAAAGAATCAGTTTCTGAATCTTCAAATAAATCAGACATTATTGCTGCAAATCAAGCCTTACAACAAGCATTAAATACTGCAAAGGCTAAAAAATCAAGTATAGATAATGAATTAAAACCTCTAAAGGATGATTTGCAAAGTAAAATTGATGAATTTGGACCTATAAGAAATACCAATTTTTCATGAATATCTAGTAGGTTAGAAACTATTAAAAATAAATTAGCAGAAGAATTAACAAAAGCAGATGCAATTAAAAATAATCCATCAAGTTCTAAACAAGCATTAAAAGATTCTTCACAACAAGTTCAAAAATTAGGAAATGAATTATTGAAGACTATAACCGAAGAATTTGGAAAAGTTGAAACTAAAAATAGTAACATCGGATATAGATTGTTTAAATTGGCACAAGCTGAACAATTTAATAACAGTGATGTTGATAAATTAAAGAACGCTTGAGAAGAAAAACAAACTCTACTTTCAAAGAAACAAAAACTAGGCAATCAATCAACAAAAGATTATTTAACCCAATTATCTACAGAAATGAGCACTCAAGAATCTACAATTAAAAAAGTTATAGTAAATATTCAAGCACATATAAGAAATAACCTTAACTCACAATATAGATTAGAAGCTGACAAGCTTATTGCCAATATGAAGAGAGGTTATGGAGATAAAGTTGGTATAGAAAGCTTACAAAAATGACAAGATCTTATGAACGATAGTGTATTGAGTGTAGATGATAGTTTGAAAGATGACTTTAACAAAGCACTTAGAGTATTAGTTGGAGATTACACTAAAAACCCTCCTGTTTTATCATGATTTATTAATAAAAGAAATAGAAGTATAGAAAATTATCAAAATCTAAGAAATTTAATTCTAGTAAGAGAAAATGAAATTTTATTAGATAAAGCAAAAGACTTAGATAAAAGAGCGGAAAAAACAATTAAATTTGTTGATGAAAATATTAATAACCTTGGTCAAGACGCTAAAAGATTAAAACAAGAAATATTAAATGCTAAGAAGGACTTAAGTAACTTTACACTAAATCATCAAAAAAATCAATTTACTGCAAAAGATATAACCCCTAAAATTTCTCTATTAGAAAATAAATTAAATGAAATAAATCAATATCTTCTTCCAATAATAAAACAAAAAGCAGTATCAAAAATATCGGAAATTGAAAAAAATAAAAAAGAACTTGAAGATATTATAAGAAGCAATTTTTATCTTTGAGAAAAAGTTGAAATTAATAAATATATTAGTGAATTAACAAACAAACAAGTAAATTTAAGATCTAATATAAATTTCGAAAATAAATGAGAATCTATTAAAGATACATTAGATAACTTAAATATTAAAGAAAATGTTTCTTTATTAAAAGAAGTAATAATAAATAATTCAAATGCTCAATATTCAATTAATAAAATATTGTTAACAATTCCTGAATTTATTAAAGTTGCTAAAACAACAGGAAGCAATAACTTACGATCACTGACAGAACAATTTAAAATTACATTGTTAGACATTGATAAGAATTTAAAAGAAGTAAAGAAAATACTAGATGAAAATAAAACATTAAATAGTGATAACATAGCCAAATTAAACGAAAAAATAAGATTACTTGTAGATAGATCTCGCGAATTAAAAGAATCATTAAGATGATTTGATAACTTCCATGAAAATATAAGTGATCAAAGGATTAAAAACAAGTTGGATGAAGTCCGTGAACTTCTTAAAAACGTTTAGTTTTGTAAGGTCTTTAAATTAAATTTAAAATTAGTGCAAAAATGCACTAATTTTTTATTTTAATGTTAATCAAAAAAATCTTGGAATTTTTTTAAAAACTTTATTTTTTTAATGTGTTATAATGATAAATTAAATGCTTATTTTTAAGTATTTATATTGCATGGGAAGGAAAATATGATAAAGAAACGAAGAAGAAAATTAGGATGATTGACCTTTATTGCCGGGGGAGTTGCCATTACTGCTTTTGTTTTAATAATGTGTAGATTTTGCAAGAAACCTAATAAGCAAATAAAAGATCGAACCTCTTTAAATTTATTTAATAAGGCGCAAAGTGAATTGAAAGAATTAATTAATTCTCCAATAGCCAAACTATGCCAAACCGCTATTGAACAAAAAGTTTTAAACGATTCAAATAAAATAAATGAAAAGTCTACTGACGAAGAAATTTCAAAGAATACAGAATATTTAAATAGTTCAATCGCTTTGCTTAAAAATAAGTTAGATCTTGCAAAACAAGAATTAGAAAAATTCAACATGGCTATGTTTCAACTAAGATCATTAGTTAATAATAAAGATAGTAAAAGTGTAGATACCAAAAATTCAATCAATGCTTTAAATAATGCTAAAAAATTGCCTTTTAATTCTATTGAACAAGTCAAGAGCGAATCTTTAACTTTGAAAAATAATATTGATGAATTAAAGTTAAAGCTTAAAGAAGTTAAAAAGTCTAGACTTGCAGAAGCCAAGAACAAATTAAAAGCAATTATTGATTCGGAACCTTCTAAATATGTTGATAGTTCTAGAGAATTGCAAATATTTAATGATTCAAATGTTGAAGCAAAATCAACACTTGATGATATTGATAAAAAGATTGATGCTGCAATAAGAGCAAGCAATGATTTAAATGAAAAAATTAAAAATTACAAATCAGCAAGATTATCTGATCTTGAATCTTCAAGAAATGATTTGTCAAACTTTATTGCAAACGATTTAAAAGATCCTGTATATAAAGAAATTGCAGATAAAATTAATGAAAAGATTAAAGAAGTAAAAGGCGTTGGAAAAAATTCAACAATCAAAGAATTGCAAGTAGCCATAAAAACTTTAGCAGATGCTAAAGAAAAAGCAATTAAAGAAAAAGCTTGGATAGATAGAGTTGGTATTAATAAAGTTAGAGAAATTCAAAGATCTAAAATATTGTTGGTTGATGCTGAATATATTGCAAGATTTTTAAGATCAGGAAATGGTGAACCATTTAGACAAAAGAATGAATTTAATGAATATTCCGGAACAGAATTTTTCAAATTAAAAAATGAGTTGGAAAGGGTTTCAAATCTTAAAGATTTAAATTCTCTTGACAGTGTTGATTATAAAGTAATATGAAAAGCTAATGAAGAATTAAAAAAATTTATTCCAATAATTAATAAAGCAATTAAATCATATTTATACGAAATTTTTGAAGGCTGATCTGATGTGCTTATTTCAAAATTAAATGTTTGAGATAAATACTGCTATTCGCCAAAATATACTGGTGCAAGTGAAAAATTAGCAGCATTTAAAAAGGAAATTCAACAAAAAAATGAAGAACTAAGGGCTAAGGCACTAGATGCCAAATGAATGGATATTTGAGTAACTTATTATCCACAATTGTTAAAGCTTCTCACTGTTGACATTCGCGATAAATTTAGAGAAGTTTATATAACTGAAGAAGCAAAGAAAAATAATCTTGCATATAAAGTTGTTGATTTATTAAATAAATTAGAATCATTTGATTTGAACAATAGTAAATTAGATAAACAAGAAATTAAAGATTTTAAAGAAAAATTAAGTGCAAAAAATCTAGAAATTGAAAAATATTTAAGTAGCGAAGAAACCTTTAAAGATTATCGCGCATATAACCCTGATTTATGAATGAATAATTTAAGTTATGATATAAAAGAACAAGAAAAAATTTTGAATGATATAAATTCTAAGATTTCTTTAATTGAAGAAAACTCATTTACAAACCAAGAATTAAAGAATACTTTTGCTGAATTAAAACAAAAATTATCAATATTGAATAAAAACTAAAGGATTAGAAACCTTTTAGGGTTAAAAAAGTATATTAAAAATAAGGCTTAAGAAATGCCTTATTTTTAATTAGTCTGGATTATTGAACATCTTTTAGTTCGCTTAAAATATCATCTAATTCGTGGATATATTGAGATGTAAAAATTTCTTTTTCATAAAATTCAGGAAATTTTTTGATAGGAACAATGTTATCTTCATTGTCTTTTTTAACTTTATAAAATTGTCAATTATAGTAGATATTTTCTCGATTTGTTTCACCCGAAAGTATAAGTCTATAAGAAATATTTTTTGAAAGCAATTTTTGCAATTTTTCTTTTTGTTTTGAATAATTAGCGTTATTTTGAATTTTTTGTAATAAGGCTTTTGCTTTTTTATTGTAATATTCAAAAACTGAATTAATTTTTTGATCTAGTAATTGTCCAAAATGATAATGAAGATACATCATGTCTTCTTCAGGGTTGAAATAATAGTCATTAGATTTTATTAATTTTTCTTCATCTATATTTTCGGTGTCATGATTTATTGTAAAACTATATAAATATTTATCTAATTTATCTTGTTTTTGCTTTTGATATGATAATAATTCAGTTTCGATATCTTTAAATTCTTTTGAAATAGTTTCGTCATTATATTCAAAAAATAAATAATCTTTATTTGTTTCAGGACTTTTAAAATAAAAATGTTCTAAAATTCTCACTTTGTGAATATAAATTTGTCATGTTCTTTCTAAAACTAACGCCATTTCATTAATTCTATAAAATGCTTCTTTTCTTTCTTCATATTCTTTAAAGAATTGCTTTATTTGTGCGGGTTTTAAATTAATAAATTTTTCTTTTGGCATTTTATATAGTTTGCTAAATGCACTTCAAAGTAATAAAAATGATTTTGGTTCTTTGAATTTTGAGTTTCTAAGTTCCAAAAATGTGTCTTCGCCAGAATCTTTATTTACTTTGTTTTCACCATTTTCCTTAATTTCTTTGCTTAATCTAGCGTGTTCTTTAATTTCTTGTTTTAAATAATTAATTTTAGCATCTGTTTCTTTTGAATATTCATCATAACTTTTTATAGATTGAATCTTTTTAAAATCCTTTTTAAATGATTCAATTTCATCTTTAAACAATTTGTAATATTCTTCCCCATTATCAATATGTGAAAAATAACTATTTTTAATGTTTTCTATTTTTGAAATATTTTCTTTATATTCAGTAATCTTTTTTGATATTGTATTTTCATATTGGCCTAAGGAATTTGAAACGTTTTTTATAGCTGCTGATATTTCAGAATATCTCTCTTTCAAGTCATTTAAATTAAGGCTATTATCAATATTTTTGTTTTTGTTTATACTGATTTCACATTTTGATATTAATGAATCTAGATCTTGCTCTATATTGTTATTTGCTTTTATATTAATTAATTTAGAATGCAAGGATTTTAGTTGCTTGACTGAAGCACAGATTTTTTCTGCATATTCATAAAATTCAATATTTTTTATATAAATGTCATTATTAAGTTTTGAATTCATACTATTTATTTTGTAGAATGAATCCATAAATTGTTTTGTAGAATTTTTAGTTGTTAAGTTATTTTTGATTAAAAAATTTTGTAGGTCATTTTTTTGTTGTATTGCAAAGTAATAATAATGTTCATTTTTATTTATAAGATTAATTAATTTTTCTGCCGATTCTCTTGAATTATTGTATTTTTGAATTCAACTATTTTTGTTCTTGATAATGCAATAGATTGAAGCGGGTATTGTGGCGGCAGAAATAATCAAAGAACTGGATAGCGCAATTAGAAATTTTTTTGATTTTTTCATCTTTCCTCCTTATTTATATTTTTGATTTTGTGATTAGTTTATATAATTTAATAAAAATTTTAATTTACTTATTTTTTAGTAATTCCTTTTGTTTTTGACCCATAATGTGGCACTTTCTGCATCTTGCTTCATATTCATTATAGTCGCCAATTAAGTTTTGTTCATTTGAAGAATCTTTTCTAAAACTTGTTGAAGCAGCATGTTGGCATACTACGCAAATTGCTTGTAATTTAGTTACTCGTTCAGCCATGGCCATCAAGGCGGGAATTGGTCCAAAAGGTTTTCTTAGATAATCTTGGTCTAGGCCAGCTACAATTACTAAATATCCCTTATTTGCTAAGTCATCAGCGACTTCAATTAATGAATTTCCAAAAAAATGGGCTTCGTCAATTATTATTGCCTTATATTTATCTTGTTTAAGTAATTCATAAACTTCGTTAATGTTTTTTAATGAATGTGTTTTTCTTCTCACACCACAACGGCTTACTATTTCATCATCAGAAAAACGATTATCATATTCGGGCTTTATTAAAAGCGGTAGCATCTTAGCATATTCTAAGGTTCTAACTCTCCTTAATAATTCTTCACTTTTTCCACTAAACATTGGTCCAGTTATAACTTCTATCATTCCATCATTAAATGTTTTATACATTTTTATACCTCAAACTTTTAAATAATAATGTTTTAACATTATAATATAATTATTACTTTTTTAAAGGAGTTTTTATGAGTTTAAAAGCTGGTATAGTTGGATTGCCTAATGTTGGCAAAAGTACATTGTTTTCTGCATTAACATTAAATGAAGCTGAAATGGCAAATTATGCTTTTACTACAATTGAACCTAACATTGCAATAGTTAATTTAGAAGATCCAAGGCTTTATGAATTAGCTAAAATAGTTCAAACAGAAAAAATTGTTCCTGCTACTTTTCAATTTGTTGATATAGCTGGATTAGTTGCAGGAGCTTCAAAAGGAGAAGGCTTAGGCAATAAGTTTTTAGCAAATATTCGCGAAGTTGATGCAATTGTTCATGTTATAAGATGCTTCGAAAATGCTGATATAATGCATGTTAATAATAAAATTGATCCTATTAGTGATTTAAAAACTATTAATATTGAATTAATATTGGCAGATTTGCAAACTATTGAAAATGTAATCAATAGAATTGGTAAAAAAGCTCAAAATTCAGGTGATAAAGAATTAAAATTTGAATTTGAAGTTGCCTTAAAAATACGTAATGCGTTAGAAAATGAAATTATGGTTAAAAACATAGATTTGAGCCCAGAAGAACAAGCCGTAATCAAATCATATCAATTACTTACTGCAAAACCTACAATCTATGTTGCAAACCTTGATAGTGATAGTTTTAAAAATCTAGAAAGCGCTAAATATTATCAAGAATTAAACAAATATTTGCAAGACAAAAAAGAAATTTTAATACCTATTTGTATTAAATTAGAATATGAATTGTGTAAATTTAATGATGAAGAAAAAGAAATGTTTTTAAATGAATATAACATTGAATGCTCCGGATTAAATTTAATTATCAAGAAAAGTTTTTATTTATTAAATCAAGCAACATATTTTACGGCGGGCAAAATCGAAGCAAGGGCATGAGTATTTAAAAAAGGAATGAATGCTGCTCAATGTGCAGGAATTATTCACACTGATTTTGAAAAGAAATTCGTAAAAGCAGAAGTCATTAAATATGAAGATTATGTTGCTTTAAATGGCGAAAAAGGTTGCAGGGAAGCTGGAAAAATAGCAATTGAGGGCAAGAATTATATTATGCAAGATGGTGATGTTTGCTTTTTTAGAGTGGCAAAATAAAAATTAAAATTAGGGGAAAATACCTAATTTTTTTTGTTGTATTTTTTTAAAATCGTATAATAAAAGAGTAATGATACTTATCATAATGATATGATATGAATTAACGAAATATAAAACTTGACCAAATTTATTTGCTATGGTTTTAAAGTTAATTGCATGATTAAAAAGTTTATTGATATACTATCAAAACTAATACTCATTAAAAGTTTCATTACATCATAAAAATTTTATTAGTCTATATATTGCTATATAGTATTTTTTGGTATTACATTAAGTTATTTGTAAGAAACGAACTTTTAAATTTCAACCTATTTTAAGGAACAATTATGAATTTAATAAAAATTGATTTGCATGTACATTCTCAGGATTCAAGCCATACAGGAAGTAATATTTCTCAAGAATCTGATTTAGAAAAATTGTTAATTTTAACTAAAAAAAGAGTCAAAATTGCTAGTTTTTCTGACCATGATAATTTTTATATTGAAAGTTATTTGAAACGTCTTGAAATAATCAAACAATACAATATTGATATTACATTATGGCCTGGTATTGAAGTTAATTTAAGAAAATATGATGGTCAAATAGGACAAGCTATATTTATATTTGATCCAGATAGCAACTTAAAAGAATTAAATGAAGTAACTAAAAAGGAATTTAGATTCAATAGCAATAAATACACATACAAAGACGCTGTTGAACTATTTAAAAAAAGCAACTTCAATTTTATGGTTTTCCCTCATGCTGGAAAAGGTCAAGATAATATGGAATGACAAGATATATGCAATAGCCAAGTTGATGGGCTAGACATAACAGATTTTAATAATAGCAACAAAAAGAAAATATTAAAACAAGATCCAAACATTCCAGTTTTATATTTTAGTGACACACATACATGAAAAAAATATCCTCAATATGGTAAATTTTGTTCTTATGTGGAGGTTGAAAATAAAGATCAAATTACATTTAATGAAGTAAAAAACAATATAAGAAATAATAAAGTTGATTATGACAAAGTATAGAAAGGAATAATATGATAAAAAGCCTAAAAATTAAAAATAATAACGAAACTTATGACATAAATTTAAATAGTCAAGTTAATGTTATTGTTGGTGAAAAGGGTTCTGGTAAAAGCACCTTGCTTTTGATTTTAGCAGAAGCAATAGTAAATAAAAAAATATTCAAAAACGAATATGAATGATTCAATGAAAAGGCTAAATTTTTAGTCGATTCAATAAATATTGATAATACAGAAATTAAAACACAGGATTTTTCATATATTTTTGATTCAGAAAATAAGCAAAATTCAAAAGATGCAGGAATTTTAGAAATTCAAAAAAACCTAAGCGGTTATATTAGTCAAAATGATAGCAGGAAAAATTCTCTTGATTCTTCTGAATATGTTGAAAAGCAAAAAAGTAATTGTATTGATAATTTCACAGAAAAAATTTACTATGATCAAGATCAAAAAATGTTAAAACAATTAGACGATTTTAAATTGCTAGAAGATTTGTTAAATGAATATAGGCGCGAACGCGATAGACAGATAGCACTTTCAAATGTTTTTAATCAATCTTTTAATTTAAATGAAGGTAGTACTAAAAACATTGCAACCATTGACTATGATTCTTCAAATGAAATAAAAAAAATTAAGGAATTATTGAAATCAATTGAAGAAACAAGAGAAAAAATAAATAGTACAAATTTGGTTGTGAATAATCTTTTGTCACAATTTGAACAATCTTCGTCGCTACTTCAAATTTCAAATGAACAATTATTGGATATTCAACAAAGAACAGAAAATGTTATACTACAAAATAACTCATTTCTAGAATATTACAATCAATATAATTTAGTTGTATCCAAAGATAAAAAAACTCTTGAATGTTTTAAATTATCATTTACAAATGCTAAAAGAGCATATCTTGAAGAATTAAGCAACACTTTGAAGATTCAAAATGATAATGAACAATTATTGGAACATTTTTCTAATTTAGGAAGAATAATGAAAAAAATCAATAGACAATATAATTCAATAATTGAAAAAGAAATCATTTTAAACTGAACATTAGAAGAAACTGATAAAAAAAATAAGAATATAAAATATAAAATAGAAAGTTTTGCTTTAAGTGATGACGAAAAAAATTTAATTTTGAATAAATGACTCGGTTCAACAAATAAGAGTAAGACAATCGCTGATGTTTTTAATGGTAATATTAAAGATAACTTTGATATTAAAAAGATGATTAAGACATTGATTAAAGATAAAATAAAAATCTATGCAGGGGATGAAGAATATAAAAATCTATCAATGGGCCAAAAGACATTGTTTGGTATAACACACGCGATTGATACATTGAACAATGTTCCTAATGAACAATACTTATTGTTAGACCAAATTGAAGATAATCTAGACAATAAAACAATATATGAAAGAATAGTTCCAATGTTGCAAGAACAAATTAAAAAAGGCAAACAAATTTTTATCGTTACACATAACCCTAATATTGGAACATTGATAAAAGGCAATATAATAACAACAGATATTTTTAATACTAATTTAAACAAAAAATTTGTTGTTAATGAAATAATTCGCGAAGATAATGAAATTGACACACCTCAATCGCTTTATTTGGAGGGCAGTATGAAAGCCTTATATGAAAGATTAAAAGTACATGAAGAAAAAATTAATAAAACTAAAGGAGATAAATAATGATAACAATTGACTATGTATTTACAAAAGATGAAAAGAGATTAATAGTTATTTCTAATGCAAGCGATAGCAAAAATAAATATAAAATTGAAATTGACTTAGACAACCCTTCTGATGCTTGAAATAAAGAAAACATTAATAATTTTATAATTAGAGCAATATCTATTAGTGACGAAAAATTAAGTGAACCACAATTAACAGAATCGGCTCAAGAACAATTACAAAAGGGTAATAAACAAATAGAATTTATTAAAAATTTATTCACAAATTTTGTTGAAAGATATAATGAAAATTAATTTTTATTCAATCTTAAATAAAGCACAAGGTTTTTAATAAAAACATTTATTTTTAAGTTGAAAATTAAAAAAACAAGGCCATGAAGGCTTTGTTTTTTTAAAAATTTTACATTTTTTCAGGTGCTTCAATGTCCATTAATTTTAAACCATCAGCTAAAACAATTTTTGTTGCCTTTACTAAGGCTGCAAGGCTTTGTTCGTTAGGAGATCCTAAAACTTTGGTATTTGAATAAAATGAGTTAAATGCCTTTGATAATTTTAATAGATAAGGAGGCAACAAGTTTACTTTATAAGTTTTGGCCATTGTTGAAATTAATTCAGGGTATTCTTTTAATTCTGCAACAAGCTTTTGAGCATATTCATTATCCAACTCATCAGAAGCAATAGGGTTTTTAATTCCTACTTTATCCAATAATTGGCAAGCACGAGCATGAGCATATTTAATGATATACATAGGGTTATTTTCATTGTTGTCAGTAACTTTGTTAATATCAAAGTCAATTCTTGAATTATATGATCTATCAATTAGAAAGTATCTAATTGCATCATTTCCAACTTCTTCTGCTAGTTCTCTTAACGTTAAAGAAGTTCCTAAACGTTTTGACATTTTAACTTCTTTACCATTCTTCATGATTCTAATTAGTTGGAATAGAAGAATTTCAATTTGGTCTCTTCTATGTCCTAAATCTTCAAATGAAAATTTAACACGTTCTACGTATCCTGAGTGGTCAGCTCCTCAAATATCAACAATTTTTCCATTTGGATCATTTAATTCCAAAAGTTTTTGTTCGTGGTATGCAATGTCCGCACAGAAGTATGTTGAATCACCATTGCTTTTGATAATAACACGGTCCTTGTCATCTTTGCCACCCTTTGTTGTTTCAAGTCAAGTTGCGCCATCTTTTTTGTATGTTGTCTTTAATCTGTGAATTGCTGGTCATACTCTATTTTTGGCATATTGTTCTGCCTCTGATGTGAAAATATCGAATGTTACTCCAAATTTTTTCATATCTTCAATAATATTTTTTAAAGCAAAATCTCTTCCAAAACTTCTAAAGATTTTTCTAATTTCATCAGTATATTCTACATTTTTGTATTCATCTTTGTGAATGTCATAAAATGCTTGAGCGAATTCTATCATATCTCCGCCATGGTATGAATCTTCTGGCATAGGAAATTCAGGATTAAATATTTGTTGGTATCTAGCAAATATTGAATCTGCCATTTTATTCATTTGGTTTCCATAATCATTAACTAAATATTCTCGAACAACGCGGTGACCTGCTTTTTCTAGAATATTGCACAATGTTGCACCAATAGCAGCATTTCTTGCATGTCCAATATGCAAATATCCTGTTGGGTTAGCAGACACAAATTCAACATTAATTGCTCCTTTACCTTTTGGATCAATACTTCCATATGTTGATCCTTCGTTGTTGATTTGATTTATTGTGTTGGCTATAACTGAGTTTGAAACTCAAAAATTAATAAAACCAGGTTCGGCAACCTTTATTTCACTAATTTCTTTAAATTTATTTAAATCAATATTTCTTACTATTTCTTGGGCAATATCTATTGCCTTCTTACCTAAATCCTTTTGCAATGTTAGGGCAATATTGCTTGAATAGTCGCCGTAATTTTTAGATTCGGTAAGTACAATAGGCTTAGAAATATTTAATTTTTCAAGTGCTTCATTTAATGAAGCAATAATAATGTCTTTCGTTGTTTTCATATTCATAATTATAATATAATAAAATTTAAAAACATTATATTTATAGTTATAATATTGAGTATGAAAAGATATTATTTATGTGGCCCAACTGTTTATAACTATCCCCATATAGGCAATTTAAGGCCAATAGTTAGTTTTGATTTAATGATTAGGGCTCAAAGGCAATTGGGAGAAGATGTTTATTTTCTTCACAATATCACTGATATAGATGACAAAATCATTGCTAAGGCAAAACAAGAAAACAAAACAGAAAAAGAAATTTCAAGCTTTTATGAAGCATATTATTTAAATTTATTTAAATTATTCTCAATTGAAATGCCAAATAAGATTGTAAGAGTTACTGATTCACTTAATGATATGTATGAATATATTCAATCATTGATTGAAAAGGGCGCAGCATATCAAGTGAATGGCAATGTTTATTTTGACGTTTTAAAATTTAAAGATGTGTATGGAAAAATAAGCAATCAAAAATTGGCAAATTTAATTTCAGATTCAAGCAATGAAGTTGACAAGCGCTCGCCATATGATTTTGCTTTATGAAAAGACACAACTGATGGTATAAAATTCCAAAGTCCATTTGGTCTTGGAAGACCAGGTTGACATACTGAATGTAGTTGTTTTATAAATAAATATTTCAAAGGCAAACAACTTGACATTCATGGAGGAGGTATTGATTTAATATTTCCTCATCATGAAAACGAGAACATTCAACATTTTGCAATCAATGGCCAAGATATTGCCAAGGATTGGATCCACTTTGGTACATTGAATTATAAGAATCAAAAGATGTCTAAGTCAATAGGAAACATAATTTATGCTGAAGAATTTTTAAAAAATTATTCGGCTGATACTTATAAATTGTTAATGCTATCAACAAGTTACAGCAAACCAATTAATATTACTGATGAATTACTTGCATCGCTTCAAAAACAAATTAATAATATTGAGGTACATTTTAATAAAAGTCAATTGGAAGATATTGAATATACGTTAGATAAACAAACAATAGATTCAATTTTAAAAAATGTTTCTAATCTTGATTTTTCATTAGCATTTAAGCAACTTAATGAGCAATTAAAATCACCACTGAATTATGGTAATGCATTTTTTGTATTTAAGGTTTTAGGCTTTAAATTAGTTGAAAATAAAGTAAGCAATGATGATAAAGAACTATATAGAAAGTGAAATATTTTAAAAGAACAAAAACAATACGATAAAGCAGATGAAATAAGAAACATTTTAAAAACAAAAAGGTTGATTTAATGAAAAAGATAGTTTTTGGAAAAAATTCAGTCTTAGAAGCAATTGAATATGAATTTCCTATAGAAGAAATTTACTTGCAAAAAGGACTTGTTAACAACAATTTAAAATTTAAGAAAGTTGTTTATTTAGATAGAAATAGCCTAGATAAATTAACAGAAAATGGAAATCACCAAGGATATGCTGCAAAATTAAAAGATTTTCAATATCATGATTTGGGTAGTATTTTTAAAGACGGCTCAGATAAGGTTTTAATTTTAGACCATATCCAAGACCCTCAAAATTTTGGTGCTATTATACGTAGTGCCAATGTGTTTGGCTTTAAACATATAATAATTCCAAAGGATAGAGCATGTGATGTAACTCCGAGCGTTTTAAAAACTAGCTCAGGGGGTTTTAAAAACGTTAAAGTAATTAAAGTTAACAGCTTATTTGATGCAATTGAGGAATTAAAAAACAAAGGTTTTTGAATATATGCATCGGCCTTAAACCAACAAGCAAAAAAACTAAATGACGCAAAATTTAATGAGAAAATTGCAATTATTGTTGGAAACGAAGGGAGTGGCGTTTCAAAAACATTGCTTAAACATTCGGATGAAATTATTTACATTGAGCAAGATAAGGAATCCGTTCAATCTCTTAATGTTTCAGTGGCAACAGGAATTTTATTATATACAGTTTTTAATAAGAAATAAAATAGCAATTTAAAAAAACAGCGGGTCTCGCTGTTTTTTTGTTTATATAGTTTTTTAATATACGCTTAATTTAAAGTAACTTTTGTACCGATTTTTTCTGCTAGAATGTTTTCAAGATCTTTTAAATCACCAATAAATGCTGCTTTTCCGCCTTCTTCAACGAATTTAATAGCTGCTTGTACCTTTGGTAACATTGATCCTGGGGCAAATTGTTTTTCAGCAATGTATTTTTCAAGTTCTGCTTTTGTAGCGTGTTTTAGATCTTTTTGATCTGGCTTGTTGTAATTTACTTTAACATAATCAACTGCTGTTAGAACAATAAAGTAATCAGCTTTTGCTAAAGCAGCCATTTTTGCTAAAGCGAAATCTTTGTCAATAACACCATCTACACCCTTAATAAAGCCATTTTCATCTTCAACAGTAGGTATTCCACCACCACCACCAACGATAACGGTAGCACCTGATTCAATAGCTTTTTTAATTTGATTAATACCAACAAAGTTTAATGGTTTTGGAGATGCTACAACTTTTCTGTAGCCTCTACCTGCATCTTCAACAATTGTGCTATTTGGGTTTGCAGCAACTGCTTCTGCTTCTGTTGCAAAGAATGGGCCAATTGGTTTTGTAGGATTTTGGAATGCTGGGTCTTTTGGATCTACAAGAGTTTGAGTTAAAATGTATAAAACTTCTTTGTTCTTTAATCCTTCGTTTTTAAATGCATTTGTAATAGCATTAACCATATGATATCCAATGTATCCTTGGCTCATTGCACCTGCTTCAGGCAATGGAACTAAAGGAGACTTAGGATTAACTGAGTGAGCACTAACGAAACCATTGAAAATCATTCCAACTTGTGGTCCGTTTCCGTGTCCGACAATAACTTCATGTCCTGCTTTTACTAATTCAGCAATTTTGGCTGCAGGAACTTTAACAAGTTCTTTTTGTTCTTCGGGGTTATTTCCTAAAGCGTTTCCGCCTAATGCAATAACAATTCTACTCATATTAGTATCCTAAAGTAGCTAGAATAACAGCCTTAATTGTGTGCATACGGTTTCCAGCTTGTTCGATTGATTTGTTGTATTTTGATTGGAATACTTCATCAGTAACTTCCATAGCACCGGTTGAAACAACTGGGTATTTTGCACCTAAGGTTTCTTTAATAGTTGCTGAGAATTGAGTTTGGTCATCGTGGAATGCTGGTAAGCAGTGTAGGAAGATAACATTTTCTTTTGCTGCTTTGATCATTGCCATATCAACTTGGAATGATTGTAATTCTTGAATACGTGGTTCAAATAGTGAGAAGTCTTCTCCTAATGATACTCATACGTCTGTGTAAATAGCATCTGCGTTTTTAGCAGCTTCTAATTTGTTATCTGAAAATGATACAGAACCACCATTACGTTTGAATAGTTCTTGACATGCTTCGTAAACAGCTTTGTGGTCTGGTCCATTTTTAACAATTTCTCATTGAGCTTTTGGTCCACATAATACAATATTAACACCAAAGAATGCAGCACCGATCATTAATGAACGAGCAACGTTGTTTTTAATATCACCTGAGAAAACAAGTTTCTTTCCTCTTAGGTCTCCAACGTGTTCTTTAAATGTTAAGTAGTCAGCTAGCATTTGTGTTGGGTGTTCAGCATCGGTTAAACCATTTCATACAGGAATTCCTGAGTATTTTACTAATGCATCAACATCTGATTGTTTGAATCCACGGAATTCAATACCATCATAAATTTGGCCTAAAACTTTAGCTGTGTCAATAACTGATTCTTTTTTACCAAAGTTTGATCCTGTTGGGCCAATGTATGTACAGCTTGCACCTAAATCAGCAGCTGCAACTTCAAATGAACAACGAGTTCTTGTTGAGTCTTTTTGGAACATTATAACAATGTTTTTTCCAACTAATGGGCGATTATTTGTGTGTAAACCAGCAAATTTTGCTCTTTTTAGTTCTAGTGATAAATCTAGTAAATAGTTAATTTCTTCGGTTGTAAAGTTTAGAGCGCTGTCTAAACTACGACCCTTTAAATTTAATGGCATATTTTCTCCTTAATTAAAATAAATTTATTTAATAAAAAATAAGTGTGTGTTTTGAATTTTTCAAGTTTTTTAAAACACGTTATAATTATACAATGTTTTAAAAATATAGATTTTTTATTTTTCATTATAATTTTTATAAAAAAAATAAGATTATTTTTCAATAACCTTATTTTTTATTTCTTTAAAAAATGTTTTATTTTAAATCATTTTTTAAATCTTTTTTATTCCCAGAATTTTTCGTTTCTAATAAATTTTCATTTAATTTTTTTAATTCATTTAAAATTAATTCTTCTGTTGTTGGAATAGGTTCTTCCTTTATTTCTTCTTCAATTGGGTTCTTTTTAGCTTTTGCATATAGAATAGAATTCTTAATTGTAAATACAATCATTAATCCTATAAAAATAAATGTTGAAACAATAATAAATTGAATTAAAGCACCCAAAAATTTACCAATAAAAATTCCACCAGTTTCTTTTGTTGCATGAATGCCTGGTCAAACCACTCAAGCATCAAGATTTTTCATACCGACTGCCTTTGTGATTGCTGACATAATAACATCATTTGCTAGTGATGATACTAGTGCCCCAAATGAAGCTCCTAATAAAAGCCCAATTGCTAGCATGAACATGTTTCCACGCGAAACTATTTTTTTTGCATCTATATATGACTTTTTAATATAATGCTTTTTTTCTTCTAATTCTTTACTTGTCATATCAATATTATATAGAATAAATTTAATTTGAGTTTTTGTTTTTTAATTAATAAAAAAAATAATAAAAAAATGGCGGGTAAGAGAGGATTTGAACCTCCGCGGGTGTTTTAGCACCCCTACAGTCTTAGCAGGACCGCCTCTTCGACCACTTGAGTACTTACCCATTTGCGCCATTGCATTAAAATTATATATAAATAATTTAAAAAAATAATAAAAATAAATCAAGGAAATTAATTTTTCCTTGATCTTTGAGGGTAAATTTTATTGATATCATTGCCACTATCAAAAATTCCTAATTTTAAATTTTTAGCATGATTAGAAGCTTCTTGTAATTGTTTGTAGTATTCAATGTTCTTTGTGTAATAAATGCTTTGAGGATTTGTACTAATATAATACATTCTTGCCATTCCAAAGAAAACCAATTGTTCATTTAAATTATAAATTTTTTTAGTTATTTTGTCTTGATAGTAAATAATTGCAACAATTCTTTTATAGTTATCATAAAAATTTTCGTCTTTTGTATTAACCCTTGATTTAGTTTCTTGAGGGACTATTCAAATTTTAGCAGCATTGTTTAGAAAATTCTTTGTATAATTTTCGGCAATTTTTCCATATTTATATTGAATGCCAGATGTTTCTTCAAATTTTCCATTAGTTATTTTATGAGTTTCTGGTGTATCAATTCCATTAAAGCGATATTTATTTCGATAGGAATCTGAGATTGTATCACCATCAGCAATTCTTGCAATATTTATTTCTGTTGCTTTTCCTACTTCAAATTTATTATTTTTTAGTATTTCAAAATCAAATTTAGCTTCATGAAAATTATTGATGTTAAATTTGTCATTATTTGTGTCGTTTTGATTATTCGACTTAGCTATCCTAGTAAGTTTTTTTATTCGTGACGACAAAATATCAAATTCTTTAATTCAATCAATTTTTTTATTGTTTGAATTTATTAATTTTTTGGCATCAGCAATTAATTGTTTATAATAATCATTTTTTTGCCATTCGTTGCTATTTTCAATTTCCTTTATTTTTTTGTCATATTTTTCTTTAAAATTTTGTTGTTCCTTGTCATTAATTTTTTCTAGTTTGCAAGAATATGAAACTATTGAAATTGGCAAAATTGTAGCAATAGAGCTCAATGCAAGATTCAATATTTTTTTAGTTTTTCTTTCCATATTTATTCAATAAGTCCAACGTACATTTATATATTGCTTGATTTGAATTTGCATAAAGGATTCTTGGATTGTAATTTTTGTTTTCCAAAAAATTATTATTTTTTAAAAAATCATTTAGGGCTTTTGCATTGGCGATTTGCAACTCAGTATTGAAATTGATTTTTTTAATGCCTAATCTAATTGCTTTTTTAATATCATCTTCTTTGATCCCACTTGCTCCATGTAAAACTAATGGAATTTTTATTTCTTGATTTAATTTGTCTAATAGTTCAAAATCTAGTCCCTTTCAATTACTTTCATATTGACCATGAATATTTCCAATTGCCACTGCTAATAAATCAATTCCAGTATTTGCTAATTTTTTAGCATCTTCAATGTTTGTTTTTTGAATATCAAGTGATTTATTTTTACTAATATCTAGTCCAATTCTACCTACTTCTGCTTCAACAGTTATATCCATTTTTTTTGCAATGTTAACTATTTTTTTTGTGTTCAATATATTGGTTTCAAAATCTTCTTTGCTACCATCATACATTATTGACTTAAAGCCGGCATTTATAGCATTAATACAATCGTTATAATTACCATGATCTAAGTGCAATATGACATTAGTTTTAATTTCTAAATCTTGAATAAGACCATTAACCAAATTGACAACGGTTTTATAGCCACCCATGTATTTAATTGCAGAAGGAGAAGCTCCGAGTATGATATCTAAATTTGCTTCTTTTGTTGCTAATAATACGAATTTCGCTCATTCTAAATTGTTTATATTTATATGCAAGATTGCATAATTTTCCTCTTTTATTTTTTTAAGTGTTTGATTTAAATTTTGTATTTCCATATTTTTTCTCTTTAATTTTATCATTGCAATAGTCATTTTAAAACAATAAAAGAAATAAAAAAACTAGCATACACTAGTTTTTAAATTCAGGTCTTTTAGAAGTTCAAAGTCCATTTACTTTTTTAAAAAAACGCCCATCTATTGTTAGTAGTTTATATAATTCACCACGTTTTTTTATTAATAGCTCTTCATCAGAAATACTAGATGTTAATTCTTCTTTTCAACGTTCAAGTAGTTCGTCTTTTACATAATTAAATAAAGTTTCAAAATCTAGTGCATCGTGTCTATCTAAAATTTCTTTGGCTATATCAACTTGCGATTTATATTCCATAATTACCTCATAAAATTTGTTTTTTATTATAACACTTTCTTACAATAATCTAATAAGCAATTCTCTATATATTATATAGATATAACTTTCATAATGACAAAAAATGTACATAATGGCGAAATTTTAGTTAACTCTAGTTAGATTTTTTCAAGTAAGTATATTGTATTTTTACTATAGACCATGAAAATATTTTGAATTTTACTTTAGATTCAAAATATGGAAATAAATAATTGTTTTATGTAGAATCTTTATATATTTTAACTTTTTTAACATTAGCCTGCCTAATTTTAAAATATGATGCATTTTATGCATAAAAAATGTTTTAAATTCTTTGCAAATTTATAAACAAAAAAATATAGTATAATTTATTACGTATAAATCATAATTAATGCAATAAAAGTGCATTTGTATTTATACGCTATAACATCTATATTTGAAAGGAATAAAAAATGTCTGTATTTGACAGTAAATTTAATGGCATTCACGTTTATTCAGAAATCGGAGAACTAGAAACTGTTTTAGTTCACGAACCTGGACGTGAAATTGACTACATTACACCAGCTCGTTTAGACGAATTACTATTCTCAGCTATTCTAGAAAGCCACGATGCTAGAAAAGAACACCAATCATTTGTTAAAATAATGAAGGACAGAGGAATCAACGTTGTTGAATTAACTGACCTAGTTGCTGAAACATATGATTTAGCATCAAAAGCAGCTAAAGAAGAATTCATTGAAACATTCTTGGAAGAAACAGTTCCTGTTTTAACAGAAGCAAACAAAAAAGCTGTAAGAGCATTCTTATTAAGCAAACCAACTCATGAAATGGTTGAATTCATGATGTCAGGTATTACTAAATATGAATTAGGAGTTGAATCAGAAAACGAACTAATCGTTGACCCAATGCCTAACTTATACTTTACACGTGACCCATTTGCATCAGTAGGTAATGGTGTTACAATTCACTTTATGCGTTACATCGTAAGACGCCGTGAAACACTATTTGCAAGATTTGTATTTAGAAATCACCCTAAACTAGTTAAAACTCCATGATACTACGACCCAGCTATGAAAATGTCAATTGAAGGTGGTGACGTATTTATCTACAACAACGAAACATTAGTTGTTGGTGTTTCAGAAAGAACTGACCTAGATACTATTACTTTATTAGCTAAAAACATCAAGGCAAACAAAGACGTTGAATTTAAACGTATTGTTGCAATTAATGTTCCTAAATGAACAAACTTAATGCACCTAGATACATGACTAACAATGTTAGACAAGAATAAATTCTTGTACTCACCAATTGCTAACGATGTATTCAAATTCTGAGACTACGATTTAGTTAATGGTGGTGCAGAACCTCAACCACAACTAAACGGATTGCCACTAGACAAATTATTAGCTTCAATCATTAACAAAGAACCAGTTTTAATTCCTATTGGTGGTGCTGGAGCAACTGAAATGGAAATTGCTCGTGAAACAAACTTTGACGGAACCAACTACCTAGCAATTAAACCAGGACTTGTTATTGGTTACGACCGTAACGAAAAAACAAATGCTGCTTTAAAAGCTGCTGGTATTACTGTATTGCCATTCCACGGAAACCAACTATCTCTAGGTATGGGAAATGCACGTTGCATGTCAATGCCTCTATCACGTAAGGATGTTAAGTGATAATTTATAAATATTTATAATAATATTTAAATTTATATAAATAATAAACGGACTCACAATAGTGGTCCGTTTATTTTATATGCTTTAAATAAATATTTATTTAGGTTTGGTTAGTTTTAATAATTATTTTTTATTCTTTCATAGTTTTTATGATTTTTTTTGGCATAGGCCGTGCAAATCATATCGCGATCAATATTCATAATTTCACCTAATCCCAAATAAACACCATATGCTTTTTTTATTAATCTTTTATTATGGTGCAAAAACAATTTGGTAAAGCATTGATATGTATAAGCAAGTTGCAAGGTAAAGTCATTATATTTAATTATTGGCTTTATATCAAATGAGGTTATGTTATTTTTTAATGCGATACTAGTAATAAAATGAATTCCATCTGCATATTCTTCTAACATTGAGATTTGGTTTATACTCTTATCTTTTTTTCAATATTTAAATAGTTTTACTTCGTTTGCAAATTCTCCTAGTTCAACAAGCAAAGCAATAACCATTTTAATTTTGATATTCAAAAATTCTGTTTTTTCAATGCTACTTGCAAATGCAAGGTCTAATTTCTTTTGGGCTTCAAAAACTTCTGTTAAATTCATGTCTTGAATTATAAACTTAAAAGTTAAAAATAATTCCATAATAAATTATTTTTTTTATTATAATTATTTGTATGCCCCGATGGCGGAATGGTAGACGCAGTTGACTCAAAATCAACCGGTGAGAGCCATGCTGGTTCAAGTCCAGTTCGGGGCACCATATCTTTTAAGACTATTTAAACTAGCAGCAATGCTAGTTTTTTTGTATTTTTTATATCTAAAAAAAGAATTTGCTAGTTATAACGAGGCATTTTACTAAAAAATAAATTTATCAATGTTATACTTAAATAGTTAATTATAAAAATATGGGGAATTAAAAATATGGATAATAGGAAGCAAGAATTAAAACAATTAAAAAAAATACCGCTGCAAGAAAATTGACCTAGAATTTTAATATTGCTAATATCTGCAATTGGTTTGCTTGTTTTGATTTATATTACATTAGTCTTTAAAAACAAATATGAAGAATCTTTTGAACTTGAAATTTTTAATGATATTTTTGTAAGTTGTTTAGTAGGAATATTTTCAGGAGCATTGTTGGTATTGGCTTCTTTTATATTGCTAGATGTTTTTAAAAGAAATAAAATCAAAAATTTCTTTGAATATTATTGTTATCTTTCTTCATTAAAAAGTAAAAATAAATTTTTATTTTTTAAAAATTATGATTTAAGAGAAATAATAAATAACAAAAATCAACTAACCAAACAAGAATTTATTTGCTTTGTTGCCGAAAAATTAAACTATACAATGCCTTCTATTGAATATAAAAATTTAGTTAATGAAATTAATAGCGATTTTGCTAAACATTCATTTTTAGATCCAAATTTTAAAAAGCAAAAGAAAGCAACACTTACTAGATTAATTATCTTTGAAATTGTCATTCCTTTATTTATTTCTATTGCATTAATAGTATTAATTGCATGTCTTTTTAACAATGATGATCAACCAATCAGGGCAATTTATAGATTGATAATCATTTTAGTCAGCGCAATAATTGTTTTAAGCCTATCGATTTTAATTTATGAACTATTTATTTTAAGTAAAGTGAAAAATTATCAAAATTTCAATAATTTATATTTACTTTCATTTAATAATTACTCATATAAGTATTTAAATTCTAGTTTAGTAAGATAGCAAAAGTACAATAAATTACAAAAATGTTAATTTATTTGAAATACTTTTTTCTAAAAGTTTTATAATTATACGTTTTAATAGGACTTATAAATGGACAATTTATGGTTTTATTAGTAATAATGAATTTTTGTAGCAATTTAGGTCTAAATTGTTAATTTTTTGTCTCCATATTAATTTAAATATATTAAAATTATCTTTGTGGAAACACAAGATTTATTTAACGCTATTAAATTTTATAAATTAATCTCAACCCTAGTAACAAGCTAGTTATTTTTTGGTTGAGATTTTTTAATTTATTTTAATTGACAAATATTAAATTAAAGAACATTTTTAATTATTAAATTGAAAGGAATTATAAAAATATGAAAAAGATAAACAAAATTCTTTTAGGGCTAGCACCTGCTGCAGCACTTATTGCCGCATCAGGACTTTCTGCTGCTTGTGGTCATACGGGTGGCGGTTCAGGAACTAGCTATGGATTTGACCAAACAGATGATGGAAAATTAGTATTAGCTTCAGGATTTTCTAACACAAATAAACAAGGTATTGCCTTAAAAGCAGTTGTTGATGCATACAACAAATGAATCAAAGACAATAATAAACAATCAGAAGGATATTTACCTGTTGAAGTTAAAACATTGGCTAATGGATATAATACCAATACTTTAAGAAGTGACTTGGATGCTAAAAACTCAAATGACTTTTATAACATGACATTAAATTATCCAGCTGCTGCTTCGATATTGTCTCAATACAAAATGAATTTAGCCATTTCAAAAGAAGATTATGATAAATTCGGTATTGAAAATGGATTTAATTTGGGTAATGATTTAATTGGTGGAAACACTAAAAAAGAAAAATGAGTAGTTCCTTTATCACGTTCATCAGAAATGCTTTCAGTTGCTAAAGCATTAGTAGGAAAATTCATTAAAGAATTAAACGGCTTGGGTGTAAAAATTTCTGACACAGACAATACTAAAGTTAAGGAATATCTAGACTATTACACAACAAATAAAAATGAAGCACAAGGTGTTGATGAAACTTGAGCACACGCTAAAACAGAAAAATTAGATGAAATTAAAGAACAAATAGTTAAAGCATTGCCAGAAATGTCAGATCAAATTTTTAGAGATTATTCAAGTATGATTAATTTCTCAATTCAAGCAAAGAAAATGTACAACAAAGATCCTTTATTAAATGTTATTGGTTTTGACTCACTACCAAATGTTATTAATACAATGACAACTTCATTAACCAATGGAGATATTAATAAAGGATATATAAAACCAGATGAAGATAAAATAACAACAGGTGGATTTGACTACTCAATATTTCTAAAAGATGCAAATAGTGAACAATCAAAATTATTCAAAAAGATAGTTGACTTAATCCTAGAAGGTGTTAAAGAAGGCGCTGTTTGAATTGGTGGTGCAGGTGCTTATGGTTCAAACAACTTACTAAAATATCAATTAGCAATGAGCCTTGGATCAACTGCCGGATACTCACATACATTCTCAACTGCTAAATCATTAACAAACTTAAAAATAAAAGATACAACTACTTCATTAGATCAATCATCATTGGAAGTTTTAGGCCAAAAACCTGGTGAATCAGATAAATGAGTTATTCAAATTAAATCTGGTAAACATACAAATGGCGTATATGGCAAAGATAATTGAGCAAAAGCTTCAAAATATGATAAGAAAATAACTCAAGAAGCTGCTAATATAATTAAAGAAAAAGTTCAAGGCCAAGCAGGATATTTAGTAGAATTATCTTCAACATTTGAATATAAAAATAATAAGATTTCTCTAAAGAAAGGTAATAAGGAATTAGATGCTGTTTATTTAGGAAAAATTTATGAAGGAAATGAAAAAGAATATTTCTTCTTAAAGGAATCGCAAGTTAAGAAAGAAGAAGTTTCATCAAATAAAATTTTAAATGAAACAGATGCTGATTGAATTTCATCACCATTAGCATTTGATAAAACCGCTAAAAAAGCAGTATTTATTCAAGGACCTTCAATTCTTCCAATTCATGCTAACGAAAAAGAAGACAAGGCAACTAAATTATTCATCAACTGAATGTTCCAACACGATTTAGAAAACTTTGAAATTGTTGAAGTTTCTAAAAAAGGAGAAACTAAAACAACATTTGACGGTAAGCAAAAACCTATTGATATTTTCAATGAATACGGAAGCTATATTTCACCAACTAAGTCATACTTCTCATCAGGTAAAGCAAGCAAATTAAATAAAGCAACAAAAATAGCATTTGACAACTTTAAACAACTTGACAATAAAGATAGTGGTTACATTCCATCAGAAGATATTGCAACTTCATTATCAGATGTTTTAAGAGAAGCAATAACAAGTGCTGGAAAAGCAGCGTTATCAAAAGCAAACGTTAACGAAGTATTTGAATATAAGGAATTTGTTTCAAAAATTTTAGGTCAATTAAGTAACCAACTTTAATAGATTTTTATTAAATATTAATATTTAAAAAACATAAAAACAACCAAATTTGGTTGTTTTTATACGGAAATTAGAGAAAAATATAAAATGAAAAAATACAATAAATTTTTATTAATTTTAGCCTCATCTGCTGGCTTAGTGGTCGGGGCTCCGTTACTGTCCGTATCATGCAAGACAGTGGATAAAGAAAAAATAAATTTATCAAAATTTGATGTAATAAAGAAAAATTATGAAATTCCAGTATATAAATTTTCAAAAGTGGGAACAATTTTTAATAAATCAATAAATTATAGAATGTATGTCACTGATAAAAAAGGAAATAAAACTATTAAAGAAGTTTCTCAATCATTATGGGATTATTATAATTACATTGAAGGCGAAGTTACTAGAATTTTTGATGGTGACACCATTGAAATAAAAGTAACAAAGCAACCAAATCCTAAACCTGGTGGTAAAGTTCTTAATGTTCCTACAACAATGAAATTAAGAATTCCAATGATTGATACATTAGAAGAAAATACGCCAGAAGTTGGAAAAGAAGAAAAGGAATTAGCAAAAAAAGATCATGCATATGCCGAAAAATTAATTCCTATTGGTTCAAAAGTTAGAGTAATTTCAAGCAATTGAAGTGAAAAAACATATGATAGATTTGTTGGATATGTTTTCTTTGGCAAAAATTTTGAAAGACAATTTGATATTGAAATGTTAGCGGGGGGATATACTCTTGCTAGATTAGATACAACAAAAGTTATTTCTGAATTTATTGCTGATTTGTCAAAAGAAAGCACTGAAGCAAAAGAAATAAGGAGCTACTTGTTACCTTATGCTGCATATGCAATTAATTATGGAATTTTAAATAGAAAAGGATTTTATGGTGAAGTTGCCAACATGCGTTCTCCTTATGAACTAAGTTCAAAATATTTAGAACACGGACAAGGTATGGTTGATTTCTCACTTTCAATACTTCATTATAACTATTGAAAGAAGCCTAAATTGGCAACTAAAAAGAATAATATCTATAGATATTTAGAACAAATTAATCAAAATAAAAAATAAAAGGAAATTCTATGAAAGAAACAAAATCAAGAGAAGAATTATTAAAAGATATTCATAGTTTTTCTAAGCAACGTCATGAAATTAAAAAGACATTGCCTGCAATTGAAATTAAAAATTTAGTTATTGACTTTGGTGAATCACTAGCAGTCGATAATGCTTCATTTTGCATTAATAAAGGAGAATTAGTAACATTATTAGGACCATCTGGTTCTGGTAAAACGACTACCTTGAATGCCATTGCGGGACTTTTAAGACCAACAGGGGGAAAAATTTTCTTTTCAGGAATTGATGTAACAAAATTCTCACCTCAACAAAGAGAATTAGGATTGGTGTTCCAAAACTATGCACTATATCCTCACATGTCAGTTTATGAAAATATTGCATTTCCTCTATACAATGACAAACATTGACGTGAAGAGACTCTAGAAAAAACTAAATTATCACAATTGAAAATTTTTGAAATCATTTTAAATGCTTATAAAGTCGATGAAAAAGCAATTGAAGATTTAAAGACAAAATGCTACTATTCAATTGACAACCCTAAAGAAACTAAAAAGTATTTAAATGAATTAATTTCACAATATAACGATATTATTGATGAACAAATTAACAAATTAAACTTTTATAAAACTAAAAAAACAGCCGAAGGAACTTCGCTAACTAAAAATATTTTGCAATATATTAAAAAACTAGAATTGCAACGCCAAGCAGAAATTTCGGAAGCTAGAGAAAAATTTTTGAGTGAAAAACAAAAACTTGATAGAGAATTTGAATTTACTCATGACAAAAATATTTTAAAAAATATATCAGCATTAAAATCAGAATTTTCAGATACTGTTTCTAAAATTAAATCTAAAAGTGCAGATGAGATTGCTAATGCTAAAAAAGAATATTCTGAAAAATATAACGAAGCGTTAGCTCGTAATACTCAAGCAATTAAAGATGCAAAACTAGAACTAATGGAAAAGAAACGCGAACAAGCAGAATCTCCATTAGTTAATAAAATAAAAGAAATAAAGAATAGATACGTTTTAGTTTCAAAAATAACAAATGTTGAATATACAGAAGCAGTTGAAAATGTTTTTGGTTCGTTGTTCAATTCTCAAAATATAACAAAAGGTAGTTTTGATACTAAACTTAAGGCTTTAATATTTGCTTTGGATCAAGAAACTCAAAATCAAATTTCAGAAATTTCTAAAGATGTTTTGACAATTGACGAAGCAATAGTAAAAGATGTATTAGATGTAGCGCAAAGAGTTGAAATTACAAAGAACTTAGCAAAACGTCCTACTCAAATGTCTGGTGGTCAACAACAACGTGTTGCCATAGCTAGAGCAATTGTTAAAAAACCAAAAATATTATTATTGGATGAACCATTGTCAAACTTGGATGCTAAGTTGCGTATTTCAACAAGAAAGTGAATACGTTCAATTCAACAAGAATTAGGAATTACAACCGTTTTTGTTACACACGACCAAGAAGAAGCTATGTCAATTAGCGACAAGATCGTATGTATGTCAACTGCTAAAATTCAACAAATTGGTTCTCCTATGGAACTATATTTGAAACCTAAAAATGAATTTGTTGCCAAATTCTTAGGTATGCCAGAAATGACAATATTTGAAGCTCAAATTAAAGACGGATATATTATTTACAACAATAAAAAACTACTTAAAGCTCCTGAGGGATATCAAAAATCTTCTATTGATGTTGGTATAAGAGGCGAATCTCTAATAGAGGAAGAAGAAGGAATATTTGAAGGAAGCATAAAATTAGTTGAACATTTAGGTAAGGAAATTCAAGCTCAAATATTTATCAAAGAATTAAATAAAATTGCAAACGTTTTCTTAGCTAAAAAACACAATTACGAATTAGGTGAAGTTGTTAAGTTAAATGCAAAAAGTGAAGGCTTATTCCATTTATTTGATGTAGACAGCAAAGAACGTGTATAATGAAAACCTATTTTTGAAAAAAATATAGAGTTAAAAACACTGGACTTGCCTTAAGCACATTAAATAACAGGGTTAGTTTTTGAAAACCCTTCTTATTGATGTTGCCATCATTAATTACATTAACTTTATTTACTATAATTCCATTTATTTTGGTTTTAATCTTTTCATCAACATATCAAGTTGGTGCAACGGTTTATGATAAGGGATTTGGACTAAGAAACTATATTAAGTTGTCAAAAGATCCAATATTCCACATCGCTATTAGAAATACATTGGTTTATGCTATATTAGCTTTACCAATTAGCTTGGCGATTTCAATTTTAATTTCATCTGCAATAAGCTTTGTAATCAAACAAAGTTTCCGTAGATTTTGACAAACAGTATTCTTTCTACCATATGTAACATCAGGAATTGCTGTTTCAATCGCCTTTGCATTCTTATTTAGAACATCAGGCGGATTTATTAACATGCTAGCGGGTAAAAACATCCAATGGCTAGATAACCCAAATGATGGTAATTGAAATGCATTTGTTGTTATTTTAATTAGAGGTGTATGAGGAAATTTGGCCTTTCAAATTCTAATTTTAACAACTGCTATGCTATCAGTTAACCCTGATTTGTATAAATCAGCTTCTATTGATGGAAGTTCAAATTTAAAACAATTTTTTGCAATTACTTTGCCATCAATTAGAAAAACAATTACTTTCCTATTTACAGTAGGAATTATTGGATCAATTAAAACATTCCCATTGGCTTTATTTGATAACAAACCTGCTGAGGCAGCTGCAAACTCTGGTATGAGTATCATGCTATATATATTTTGATATGTTCAACGTGGAGTAAATGGCGTTGCTGGAGCAGCATCATTGATACTATTTCTATTAGGCTTATTAGTATCATTTGGTTTAAGAAAATTAGTAAGCTTAGTGTTCTTTGCTAACAAGAAGATTGGAGAAATAAATGTCATTAGGAAAATTGAAAATAAAACATTGAAGAGAAAAGTCGTATTTAAGATCTAAGCAAGAGTTAATGTCGAAACCTTACTCTTCAACTAAGGCTAGTTCATTAATAGCATCTTGAGCATTTAAAATAATAATTTTAGTATTCTTCGCGTTAGTAATAATATTCCCATTCTACTTTATGATTGAAATGTCATTTGTAGAAAAACAATTAAGTACAGATTCAAGAACCACAATACTATTTCCAAGGGCAATAGTTGAAGGTGGAAAGAGCTTCTTCCCTGAAAACTATAGAGATGCTTTTGAATCAGGATTCCTTGAAGCACTAGTATTTACTGCTGGTATCACAACTTTCTCGGTATTAATTAGATTAGGATTTTCGATTACATTAGGATATGCACTATCATTAAGAAATTGAAGGGGGAAGAATTCGTTCTTTATCTTCTTTATATCGTTAATGATATTGCCGGAAGTCGCCTTACTAAGTGGTCAATACCGGTTAGTTGTAGCATTGGGTTGAACTATTGGATGAGGTCAATTCTTTGCATTAGTTATGCCGTTTGCTGCTTCAATATTCTTTGGATATATGTATAAAAATGCATTTGAAGCAATCCCAAACAGTGTTAAAGAATCATCAATGCTAGATGGCGCAAATGGAATCAAATACTTCTTTAAAATAGCAATGCCAATGGTTTCAGCAACAACCTGAACAGTTTGCATTCTTACAGCATTTGCATCATGGAACTCATATACATGACCTGCATTAATATTTAAAACATTAGGTTCACCTTGAAAACCTTTAAACCTATGAGTATTTACTACCGGTAAATCACAAGAATCAGAAATTCAAATTGTTTATACATCAATAAGAATGGCTGCAACTGTAATTGCAATTCTCCCAATGTTTATAATTTACTTCATATTAAAGAAGAGAATTATGAATGCAATTTCAAGACAAGGAAATGCAACAAAAGGATAGAATTATGGAAAAAGACATTAATGTTCAATTAGTAAAATTATCAAAATATCAAAAGAAAAGTAAAATTAAAAAAGTATTTGTTTATATTGGTATAGGATTTGTATTAATTTCTTTGATTGTTATAATGATATTGGGTGTATATGGCATAATTGGTGGTCTAGACGGATTAATAAAAAAAGGTTTATAAAAAACTGTAATAAAAAATATCGCATTTGCGGTATTTTTTATTGATATTTTTTAGTTTGCAAATATAATATTTAAACAAAGTCAATAAAAAATTTGGAGAGATATATGGCCTATGGAACTAAAAATTTATTAATATTTAAGAATAATTTAATTCAAAATAAACAAAATTTAAAATCTTTTGCACTTAAATTAAGCTTTTATGTTTTAAAACAAATGCCACAAATTAAAAGTGTTATATTGGCATATGACAATGAATATTGAAAAAAAATGAGCCAATACGTAACTTATTATTTAAAATCTAGGGGTATAAATGTTTATTTTTTACATAAGAATTATTTAGGAGTTCCTTTTTCATTCTTAGAATTTTCTTTTAAAAAATTAAATACCTTGGATTTGGCAATATATTTTTCTTTTTTTGACAGTAAATCATGTAAGATTCAAACTATTTTTAAAAATAATCAAGAGATAAATTTTTTTGATTTTAGCAATTTCAACCTCAACGAAAATGAATACCAATTTTTAAATGATGAGCCAATATATTTAGATACTAAGATGCTACTTAATGATTATTTAAACCAAATTACTTCAAATAGAGTAACTAAATATCATAATTCAATCCTTAATTTAATAATTAACAAATATCAATATAATTTTGAATTAATTAAGAAGATTATGGGTTTTGATGATTTGGGATATAAATTTATTAATAAACAAAGATTCACTAATGATACAATTTTTAGAGTTTCTAAAACTTTTTTAACTAGGGGCATGCAGTTTAATTATTTCGCTAGATTTTTTATAAATCAAGAAGTTAAATATTATTTAGCATATAAAAAATTATTTAATTATGATTTTAAACAAATTTTAAATGAACATTTAGTTGCATTATATATTAAATTTCAAAACACATTCTTTAGCTCAATGCTAATAAATGATAATTTTTTTAAAATAAATAAAGTTTATATAAACCAACTTTATCCCACTCCTATTTTAATAAAAGTTTGTGAAATGTTTGACATAGAATATAGTTTTGTTTTGCAACAACAAGACATTATTTTAAACGACAATGAAAACGTTTTGTATGTTGATAATAATGATATTTCATTTTTAAGTGCTGAGTATAATTATAGAGATTCATTTAAGACATTTTTTTATATTAATGAAATGTTAAATTATTTTGAAACTCAGCAAATTGATATTTTTGACATTGAAAAAGAATTGAAATTAAAATTCTTAACTTATAAAGAATTTGAAATTGAATGTAGTATTGCAAATTTAGTTCATTTTAAAGACAAATTAAGTATTCAAAAATCAATATCTCGTATTGAAATTGATCAATTAACAGTTTTTGAAAACAAACAAGACAAATATATAAAACATATTGCTAAGATAGCATTAAAAACCAATCCTCAATGTTTTATATCTATTAGTTATAATGAAATCTCCTGCAAGCTGAAATTTTCTGTTTTTTATTCGCAAACTACAAAAATTTTCAATAAAATCAAAGAGTATTTTAGCAAATTTCTTTTATTAAACAAAAAAATTCGCAACTAAGCGAATTTTTTAATATTATTCTTGGAACAAAAGCTTAATTTTATTAATAAATTCATTGTAATCAAAAATTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCTCTTAATTGACCACTTCTTCTAGTTGCAACATCATCGGCAGGTTTGTATATTGATCCTTCTTTGATTAAGTGTTTAAAGTTTGTGAACGCTATTTCTTCGGCAATATTTTTTGTTACGTTCTTTGCATCTTGGCTTAGATATTTATTTGTAGGAGAAATATAACCACTAAAGATATTAAAAGCGTCTATTGCTTTTGTACCATTGGGGAATCTTTGAACATTTATCTTTTTACCCTTACTATCAACTTTATAAAGCTCTAAATTATTCAATGGTTGTGTATACATTCAATTAATAAATAATTTAGTTGCTTTATCTTCTCTTTTATTTGCATGAACCGGAATTACAGAAGGACCTTGAAGAATGATTCCTCTATATTTTTCATTTTCTGAATATGAATAAGGGGGCGATATTATATCTACATCTTCTTGATTAATAAAATTATTAGCATTTAATACATTGACTTCTACATCTTTTTTGTCAATAAAGAAATATTCGTTATTGTCATTGTTAAAGAATTTTCCTAAATAAATTCCTTGAAATTTAGTTATTTTATTTCCAATTTTTAATTCGATTTTATCTTCAGCTTTGTTGTATGAATATTCAGGCCCTGAAACAATAAATCCTGCCTTGCCTTGTAATTTTTTAGCTTCTTTTGTTGATGAAGAAGATATTTTTTTAATATATCTAGGATTTTGTCCGGCATCTTTTATATCTTTTTCACTAGCATCTACTGTTCAAATTGTATTCGAATATTTATCATTCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTGGGAGTATCGTTTAATATTTCAATTGTTGTGTTTGGATTTAACACTCTGTTTGCCTGCTTGATTGTATAATTGTTATTTTTTCCATTTTCATTAACAAAAGTGTAATGATAAGCAGCGGTTGAAGTAATAACCATTGCTAATTTATAGGTGGTAAAAATGCCCGTTCCTCAAACACCTTGTTTAGCTACTCAAACGGCACCTTCTTGAATACCCTTTAAAATAATAGAAGAAACTTTTTTAAATAATTTCGCTTGCGGGCTATCAGGTTTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGTTGGACAATTATCATCTGGGTTTACATAGTTTTTATTGATATCACTACCAATGATTGATGTTGCCATAACATTGATAGCCGAAGGTATTTCATCTATTCCCAAAACGTTTAATGTAGGGTCCGCGTTGTATAATCTTTTTGCTTGAATTGCAAAATTAATTAAATCGCTATAGTTGTCAAATAATTTATCTGACATGATTGGCAATTCTTTTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATACCATCAACGCCTTTTGCTTCTTGATCGTTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTACTATTTTGACTATCTAATTTAACTCCTAAAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTACTGTAGTCATTTCTCCTGAACGAGATAGAGGTATTGCCCACTTTTTATTATATTTGTTATTTGCAATTAAAGAGTTTGCGACATTAAATACTGGTTCAATGTTATATTTTTTAAAATCTTTGTCATCAATTGCTAAATTCATTTTGTGATTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATGTTGTGAAAATCTTTCCTATTTTTTACTTTTAAATCAAGTGCAATTGAAGATGTATTATATCCTGAAGCTAGTCAGGAAATTTTTAAAGGCAAGTATCCTTCAGATTGCTTGTTATTGTCTTTGATTCATTTGTTGTATGTATCAACAATCCCTTGAATAGCAAGCCCTTGCCAATCATTTTTTAAAAATCCCATTGCTAATACTAATTGTCCATCATCTTCTTGGTCAAATCCGTATTTCGAATTTTCTGTTGTTGAACATGAGGATAAAATCAATGTAGTCGTAGGAAGTAAACCTATAGTCATTAAAGTGAATAATTTGCCAAGTCTTTTCATATAATCTGTTTCCTTTAAAAATGTATAAGAAATGAACTACAAATTCATATAACCGTGTTGTTCTCTACTGGTTTTAAAACCATGTAGTAATATAAAATATATTTTAAATATAAACCCAAATTTCTTATTTTAACTAATGAAATAAATTATATATTTTTATTGCATTAATTTTTAGAAAACGAAATTCTTTATGCACTTTTAGAAACAAAAAAATGTCTTATTAAATTAAAAAATTCGCAACTAAGCGAATTTTTAATATTATTCTTGGAACAATAGCTTAATTTTATTAATAAAATCTTTGTAGTCAAAAACTTTTAAAACATTGGCATCACTCAATATTGATCTAGCTGCTTGCTTTATTGCATCCCTTAATTGGTCGCCTTTTCTAGTTGCAATATCATCGGCAGGCTTGTATATTGATCCTTCATTGATTAAATGTTTAAAGTTTGTGAATGCTATTTCATCAGCAATATTTTTTGTTGCACTCTTTGCATCTTGACTTAGATATTTATTTGTAGGAGAAATATATCCACCAAAGGCGTTGAAAGCATCTATTGCTTTTGTACCATCAGGGAATTTTTGAATGTTTGTTTTTTTGCCATTGCTATCAACTTTATAAAGTTCTAAATTATTCAATGGTTGTGTATACATTCAATTGATAAATAATCTAGTTGCTTTATCTTCTCTTTTGTTTGCATGAACTGGAATTACAGAAGGACCTTGAAGAATGATTCCTTTATATTTGTCATTATTTGAATATGAATAAGGTGCTGCTATTCAATCTGCATCTTCTTGGTTAATAAAATTATTGGCCTTTAATACTTTAACATCCACGTCTTCTTGATTAATAAAGAAATATTCATTTTCATTATTATTAAAGAATTTGCCTAAATAAATTCCTTGTAATTTAGTTATTTTATTTCCTATTTTTAATTCAATTTTATTTTCGGCTTTATTGTATGAATATTCAGAACCGGAAACTATATAGCCTGCCTTGCCTTGCAATTTTTTAGCTTCATTAATTGATAAAGGAGATACTCTTTTAATGTATGTAGGATTTTGTTTAGCATCTTTTATATCTTTTTCACTAGCATCTGACGCTCAAATTGTGTTTGGATATTTTACACCCAATTGTTTAAATTTCAATAATCATTTTTCATTATTTTTAGGAGTATTATTTAATATTTCAATTGTTGTTGTTGGATCTAATACCCTGTTGGCTTGTTTAATTATATAAGTACTATTTTTTTCATTATTACCAACGAATGTGTGACGATATCCAGCAGTAGAACCAATATTTATAGCCATTTTATAACCAGTAAAAATACTTGATCCATAAACGCCTGGTTGAGCTACTCAAACAGCATTTTCTGAGATACCTTTTAAAATAATAGAAGAAACTTTTTTAAATAATTTTGCTTGTGGACTATTAGGATTTTCAATAAAATTGTCATAATTGTAGCCACCAGTTGCAGGGCAATTATCATCTGGGTTTATATAGTTTTTATTGATATCACTACCAATGATTGATGTGGCCATAACATTGATAGCCGAAGGTATTTCATCTATTCCTAAAACATTTAATGTAGGGTCAACATTATATAATCTTTTTGCTTGAATTGCAAAATTAATCAAATCGCTATAGTTGTCAAATAATTTATCTGACATTATTGGCAATTCTTTTAATATTTCAGGTGTTAATTTTTTTAAATTTTCTGGTGTTGTTTTTGAATATGCTCAAATGCTATCAACGCCTTCTGCTTCTTGTTTATTATTATTGTAATAGTCTAAATAAGATTGCAATTTGCTACTATTTTGACTATCCAATTTAACTCCCAGAGCCATTAATTCTTTAACGAATTTTCCAACTAATGCTTTAGCTATTGTTGTAATTTCTCCTGAACGAGATAGAGGTATTGCTCATTTTTTGTTATCTTTATTATTTGCAATTAAAGAGTTTGCATTATTAAATACAGGTTCAATGTTATATTTTTTAAAATCTTTGTCATCAACCGCTAAATTCATTTTATGGTTTGCATATAAAGCAGCTGCTGATGCGTAATTTAATAAAATATTGTGAAAATCTTTTCTGTTTTTTGTTTCTAGGTCCAATGCAACAGAAGAAGTGTTGTAACCTGCTGCTAGTCAAGAAATTTTTAAAGGTAAATATCCTGCTTGTTTTCTTTCTTCTTTGAATTTTGGATCATTTATTCAATTATTATATGTATTAACAATTCCTTGAATGGCAAATCCTTGCCAGTTACTTTTTGAAAATCCCGTTGCTAATACTAATTGTCCATCATCATCTTGGTCAAATCCATATTTTGAATTTTCTGTTGTTGAGCACGAGGCTGAAATTAATGCAGTTGCGGGAAATAATCCTGCTGCTACTGCTGTAAATAATTTGGTAATTTTTTTCATATAATTTATTTTTCTCCTAAAAAAAGTGTATAAAAAAACGAACTACAAATACAAACATTTTGTACAGTTATGTTCTTACTAGTTTTAAAAAACGCTAGTAATATAAATATATATATTTTTTATTTTTTGTTTCAAAAATTTTTATTTTTTTTTCAGCCAAAAATATAAATTTAAATTTTAAATTTTTGCTTGTTAAAATAAATTAATATATTGTTATTACATTATTTTTAAAAAATAGATTTTTTTATAAAAATTAGTGTAAAATAAGCATGCAATGACTCAGCATAAAAATATTTTTTAAATAATTTTTGCTACATTGGATTGAGAGTAAGAAAGCTGTAATCCGAAATCATTTAAGCAAAAATCTTAGGAAAGAATTTTTAAAGTCCTTGCATATGAAAGGAACTAAAAAATGTCAAAATTTGTTGGCTCAATGTTTAAAAAGAGCCGTAGATATGGAATTTCTTTATTAGAAAATAATAAAGAATTCACAAAAGGCAAAAAACGTACAACTGCCCCTGGACAACACGGAGCAAAAAGAAGCAAACCATCAGACTATCAACTACACATGTATGAAAAACAAAAAGTTAGATATATGTATGGTTTAAATGAACGTCAATTTAAAAATTTATTTATTAAAGTTTCTAAAAAAGCCGGAGTTACTGGTATTAACTTACTACAAACTGTTGAATCAAGATTAGACAATCTAGTATTTAGAGCAGGATTTGCAAGAACAAGAGCTCAAGCAAGACAATTAGTAAATCACGGACACTTTACAGTTGATGGATGCAAAGCAAACATTCCTTCAATGATTGTTAAAGTAGGTTCAGTTATTGAATTAAAAGAAGCATTACAAAATAATGTTGCTATTAAAGACGCTATTGAAGTAATGACAGTTGCTCCATGATTAACAAAAGAAAACTTTAAAGTAACATTTACAAGATTGCCAGAAAGAAACGAATTTGCTAAAGATATTAACGAATCATTAATCGTTGAATACTACAACCGTTAATAAACAAAAAAACGAAGTTTGTTCAAACCTCGTTTTTTTATTATTTATTTTTAACATAATTAACTTTTTTAAAAAATATAAATTGTTTTTCTTTTATTTTTGCAATCAAAACAATAAATAAGATAAATGCTGGTATATAAACTAAATTAATTAGATAAATATTATTGTATAAAGGGTTAAGCGTCATTGTTATATTTGAAAGTGAATATATAGAGTTTAAAATTTCAGGGAAAACTACAGAACTTAAAACAATAATCAAATAGTTTGCGAGTCTATCATGACTAATTTGATAAAAAAGAGTACCCAAAATAAGCGATATTAGATATATTAATATAATTACAAAAGTAAACAATATTGCTTTTTTGAAGTTATTGGTGATTTTTATTAATAAAAAACTTAATAAAAATCAATAAGCTAATCTAAAAATATAAACATAAAAATATTCGATAATTTTTGTATTTTCTAAATATACAGGAGTTTTTGAATGTCAATGTCCAGAGACGATTTCATAAAAATTTTGTTTAATAAACAAAATCATTACGTTAATTAAAAATATTACAATCAATGATATTGCAGTCGTCAATATTATTATTAAATAGTTTCTTCTTTTTTCTTTGTGATCAAGTTCTATGCTTCGTTTTGATAAATATCGTTTAACAAAATAGAAGTTTAAAAATAGTCCTATTGATAATAACACCAAAAGAAGCTGGGTAGTATAAGAAGGAGCGATTAAAAATATTTGATTTATTAAAGGAAATAGATTAAATTGATCGATTTGACCATCTATTTTTAATATTATTGCTGATATTAAAAGATAAATTACAAATCCGGCTAGCAATATTCAATATGGCAAAAATAGTAGTAATTGTTTTTTATTTTTTTTCATTAACAAATTTCTCCATTTCTCTATGCATGGTAAATAAATGAATATATAGTTTTAGCTCCATATGCGGTAATTGATTTTTATTTTAGCTGTTGTCAATATCTAATTATTGTTTAGTTTCTTAGATATAATATCATTTGCTTTTTCAATTCATAAACTCATTTGGTTATGAATGTAATTGTAAAATCAACTAGATTTGTTTAAATATTCCTCATTAAATTTTTTTAATTCAAAACTAAAAGTATTTGTTGCTTCTAATAGTAATGCTTCGTATTTTGTATTATCTAATGTTTTAAATAGTTGTTCAAGTTCTTTTTTTGCATCTTCTGGGTTGCAATATATATAAAAAGGTTTAATAATATTATTTTGGATTTTTGTCATAATAATAAAGCAATAATTAAGGTCTTCATCATTGACAAAATTTAATAGTTTTTTTATTAGTTTTTGAATAATATAACTTGCTTGGTAATTATCTTCATCATTTGAATTGCTGAATTTAAATTGGTACTCTGTTTCTTCATCAAATAATTGATTATCAGCAACAAATGCTTGGTTTATATCGCAATATAAACCTTGAAAATTATCAATGTCATATTGACTTAAATTATTTGATATTGAAGTTAGAAGTACTTCGTTATCGTGTTTAATTTTTGAAGCCTTTGTATAAATTGTAAATTCGATTGTTCTAACAAGAGGAATTAATTTATATTCATCAACATCATTCAAAGGCAATAGTAATCTAGAACAAATCATATATTATCTCCAATCTAATAAAAAAACCACAAGACATAATACTTCTCGATATGGCTGCTATATTTCTATCCTGACCAAGTTACAAGGTTATCATTCTCATGGCATTCTAATTATACTAAAAAAATAACTTTAATTTTATAATTGAAAAAATAATAAATTAATTTGATTCTAAACTTTGCCTTAGTTTATTTATTATCATTTTTTATATTTTACGAATTATTAGATTAATTTCTTTTTTTGCAAATAAAAACCTCCCTCATAATATTTTTTATAAAGGAAGGCTTCATAAGTTTATGTAAATTTTATAGTTTCTTATTTTTAATGGTATTAAATTTCTTAAAAAATATGAATTTTTTTTCTTTGATTTTTGCGATTAAAACAAAAAATAAGATAAATGAAGGCACATAAACTAAATTTATTAAGTAAATATTATTGTATAAAGAACCAAGTGTCATTGTTATATTTGAAAGTGAATATATAGAGTTTAAAATTTCGGGAAAAACAATGGAACTTAAAATAATAATTAGGTAGTTTAAAAATCTATCATGGCTAATTTGATAAAACAATGTGCCTAAAATAAGTGATATTAGATATATTAATATAATTACAAAAGTAAACAATATTGCTTTTTTAAAGTTATTGGTGATTTTTATTAATAAAAAAGTTAATAAAAATCAATAAGCTAATCTAAAAATATAAACATAAAAATATTCAATAATTTTTGTATTTTCTAAATATACAGGAATTTTTGAATGATCCCTTCCATCCTTTATTCTATAATAACTTTGATTAATAAATAAAATCATTATGTTAATTAAAAAAACACTAATTAATGCAATTATAGTTGTTAATATTATTATTAAATAGGTTTTTCTTTTTTCTTTGAAATCTAGTTCGATATTGTGCTTTGTTCAATATCTCTTAGCAAAATAAAAGTTTAAAAAGAGTCCTATTGATAACAACATTAAAAGAAGTTGCGTAGTATATGACGGGGCAATCAAAAATATTTGATTAATTAAATTAAATAAATTAGTATTGTCAATCTCACCATCTACTTTTAATATTATTGATGATATTAAAAGATATATTGTAAAACCACCTAGCAATATTCAATATGGCAGAAATAATAGTAATTGTTTTTTAGTTTTCATTAATAAGTTTTCTCATTTCCTCGCCAGTATGCATATTATTTTTATAATTAAACAATTTTTTAGTTACTAAATTTTGGTTAGCCTTTGGCCTCATAGTAAAAATTATTTTTAATGGTGAGCCAGAGGCTGCTAATTTTCAATAATTTACTAGTACTATAGAGTCATTATTTCCTCATAATCAATTTAGTAGAACCATCTGAGTTTCTTCATCATATCCATATATTATAAATGCGTGCATAGTTTTGGCGGTACTTACAATTACGGGGTTATTATTTTTTATATTTTCTGTTGCCTTATAATAATAAGCACCTTTTTTGTAATCATACTCATACTTATTTTTTGCTTCCTTATTATTTAAAAACTTATCTATTGGATATGTATAAGAATTATAAGCATAAAAATCTAGTCACCTTTTATTAAGCCTTCATAATTTAGCAACTAATGAATAATCAGGATGATCATATTGATAATATCTAAAAATAGGCGATGAATTTTCTATAGAATCGTCATAATTATCATATTTAAAAAAATCATTAAATTCTTCATTTGTAAAAATACCACTTTGATTAAAAAGTTCATTATAAAGTAAAATATTTGATATTGCAACATATTCACATAATCCAGAAGTATCATTGTATTTATCACTCAAAAAAGTTTGATCTACATATCCTACTTTTTGCATTGTGTTTCTAGTTGCTCACCACCAACTATTTTTAACCATATTGCTTATATTTGGCAATGTATCTAGTTTGTCTTCCAAAACCGGAGAAAAATAATCTTTCTTATATGCCATTCTTAATTCATTAAAGTTTTTATTTTCTTTTTCTAAATAATTAAAATTATATTTTATGTTTTCATTATAAGAATGAAAACCCTTTTTGATTTCAGTAATTGAAAAATCGTTTTTATCAACGATAGCATAATAGTTAGAACATTGAATGTATAAATATTCATTATTTAAATTTTTTATATATTTAAATTGTTGATTTTTTCCAAAAAATCTTTGAATAGTATTGGTTACTTTTTCTTTTAATTCTTTATAGTTTTCATTGTTGTTAATATTGCTAGACAATAAGCAACAAGGCATTGCTGCTACTATTGTTAATGCAGGAATTTTTATTTTCATCATTTGGTCTCCTAAAAAAATTATATAATTTTTTTGCTTAAATATTAAATTAGTTATGAACACATTTTATAAAATCAATTGTCTTTTTTAAAAATGTTTAATTAATTTTTTGTTGTCCTAGTTATGCTAAAAAGACACTATTTAATTTTTTTAAAATTTACTAAGTGCAATAAAAAATGCCATTCAATAAATGCATTTTTAGCAAAATATTGAATGGCATTTATTACAATAAAATTTTATTTTTTATTGCCCTTATAATTGCTATTATTTGGGTTAAACTGATTTGAGTGATTATTTCAATTTGATCTATATGCTGAATTATTTGGGTTCAATTGATTAGAATGATTGTTTAAATTTGATCTGTATGCTGAATTATTAGAATTATTTTGATTTGAATAATTATTCATTTGTTGTCTTGTATGTGTGTTTCCACTTACTTTATTATTTTTGTCATTCATAATTTACTCCTAATTTTTATATTCGTTCGGCAATCCATAATGATCACAAAGCACAATGTATGCCGCCATTTTTTTAGCAAGTTTTTTTGATGTGCCATATCCTTCTGTGTATTTAGTCCATAAGCCCATTCATTGAAGATTTCTAAAATTAACATTACATCTGCAATGTCATCATTGTTTGCCGTTATCATCAATTATAGGTTTTTCTGATATTTGATATATTGGCATTGAGCATTCACCACGCTCTGCTAATTCTTTTAATGTATTGATAGCATTTTCTAATTTAAATTTATTTGGTCTTTTTTCTCTGCAATCAACATCTCTAAAAAAATGTTCAATATCTAACATCTTTATAATGATGTTTTTTAAACGCTTAATATCTCAACCACTGTCAATTGCTATTGCACCAATTATAGCTTCAAATAAATCAGCTTTCACACTATCTTGATTAGCAACATTATTTCTTTTATCATTATCTCCTAAAACTAAATACTTAGCAAAACCTAATTTATTAATTTTTTTAGCTAGTGTTTTATTGCTTACGATTTGATTTTTTAAATCAGTAAAATCGGATTCATTTTTATTGGCTATAAGGCAATATTCTTCAAGATCTTCATATTTGTTGTAATATTGCGAAGCCGATTTTAAAAAGCCAAATTCTTCTGCCATTAGTTGAACTACGCAAAAGCTCAAAACTGAATCTCCTATAAATTCAAGAACTTCATTATTTTCAACTCCGTATTGATTTGAATAAGAACTTCTAGTAAATGCTTGATAAAGTAAAGATTTATTTTTAAAGTAATAACCTATTGCACATTCAATTTCATGAATATATTTTTGCCTTTCTTCTATTCCTATATTTGAATTTTTCCTTTCTAAAATAATTATTGTTCAATAGTTAAAATATGCTTTTGTTATATATTTTAAATTTTATTTAATTAAGGCAAAATAATTTTTATAATATAGTTCATCTAAACTTCTTTATTTAATTGTAAAAAAATGTAAATAATAGATATTATTCAACTACTTTAACATAACTAAATTATTGAACAATTTAATTATGTTTTATATTAAACTATGACTTTCTTTTATTTAAAAATAAATTTAAGCGTATATATTAATTTTTGCAAAATAATATTGCAATGTGAGTTTTATTTGATTTTAGCGTTTTATAATAATTTAAATAATATAATATTTATGTATTTAGGAGAAATTATGTCAAACGAAGAAAAAATTTATGTTTTTAGTTATGGAACTATTCAAGATCCACAATTTTATAAGGAATTATTACCAAACTCAAAGCCAATGCCCGCAATATTAAATGGTTATGCAAAATGTGTTGATGAAACAATGTATTTTTTATTAAAAAAAGACTTGTCAAGTCAAGTTAAGGGAAGCGTATTTGAAATTAGTAAAGAAGAATTGTTTTTAATTGATAGATGAGAATTATTTCCTCAATATCAAAGATTTCAAGTCAATGTTTTATTAACCGAAACTAATGAAATTTTAGAAAATGTTTATGTATATACAAAATTAGAAGTCGGAAAATATTATTTAGCAACAGATGATATGGGATTTTCTAGAAATCCTAATGCTAATGAAAATAATTTAAATGCTTTTATTGAAATGGAAAAAGCAATAAAAGATTTTCCATTAACTGACTATATATTTTTATATGACATAAACGAACAAGAATTTGAAGAAATTAATAAATTAACTCACCCTTATGCTGCTTTAATTATTGATGATAAGGAAAATAGAAATTATGTTGCAATTCATGGATCAATTTTTGCAATTAAAGAAGATGGCAAAATGTATGGAGCCTTAACTTCGTTTAGTCAAAAGTCAAATTTAAATAGTATTTTTTATTATCAAGCATTTAATGAAAAGTTATTAAATAGCAAACCAGAAATAACACTAAAATCATTATATGATAATACAAACATTGATTTTTTAATTAATAAAAAACCCGTTTATTATTTAAGCTCAAGAGAAGACAAAGCAATCAATGAAACCCAAGTTGGTTGATATGAAAATAAAGCCTTTGAATTAGTTGAAAAAGATTTTGACATTGATCCATTTATTCGCTTTAATAAAATGTTAAAAGCGTTTTTTGATACAAAACAAAAAAATGATAAATAAAGAAATAATTATAAAAAATGTGCCACATAAACCCGGAGTTTATTTATGAAAAAATAAAAATGGCAATATTATTTATGTTGGCAAAGCAAAAGATTTGAAAAATAGAATGTTGCAATACTTTGATATAAAAATGCAAAATTCTTTTAAAACAGAAAAAATGAGGCAAGAGATTGCCGATTTTTCTACAATCATTTTAAGCTCGGAACGTGAAGCATTTATTGAAGAAAGAAAATTGATTGATTTTTATAAACCATTTTACAATGTTCTTTTTCCAACTAGAGATAGTTTCCCTTATATAAGAGTTAAATTACTAAGTACTAATAAATTAATAATTGATATAAAAAATCATTATAAAAAAGAACCAAATTCTATATATTATGGTCCTATTCCTGACAACAAAAATTTCAAACCATTAATTAGATATTTGAATCATTTACTACTTTCTAAGGATGGAATCATAATTGAAAAACAAAGTTCTGAATTTGCAAAGCAGAGATTTGAACAAGCCAAATCAATTATGAAATTTGGCCCTGATTTTAAAAGAAATTTAAAACAAAAAATCAATGAGGCAATTGAAATGAACCTGTTTGAGCAGGCGAGATTTTATGACCAAATTTTAGATTTATTAAATTACAATAAACAAAGTCAAAATATTTTTATAAAAACTATCAAAGATATTGATGTCTTTGGTTTTTATCAATATCAAAACTATATGTTGGTTAATGTTATGATTTATAAAAACGGATCATTAGTAAATTCTTATGACTATATTTTTGAAATAATATTGAATGAACATGAATTTGTTAATCAATTTATTGATGAATTTTATGTTAATAATCAATTACCGGCTCAAATAATAATACCTTTAAAATATTATGAATTAGGTGGATTTGATTATAACGACAAAATAAGCGCGCCATTGAACAAATTAAACAAAAACTTGATTGATATTGCTATTGAGAATGCATCAAATAATATTGAACAAAAAATAAAGAATTATTTGAGTAAGCAAACAAAAGACAATGAAATTTTCAATAAATTATGTTCATATTTGAATGTTTATGCAGAAAGAATTGTTTTATTTGACAATTCATTTTTATCAAATACTAACGAAGTAATAGGAGCAGCTTGCTATTTTAAAAATGGCCAAGCTTTTAAAAAATTCTACCGTTTCTATAATTTAAAATTGAATTTATTAAGAAATGCTGATGTTGAATATATGCAACAAACTTCCTTTAATTATTTAAAAGATTTTGGAGATCAAGTTGATGTTGTTATTGTTGATGGTTCAATTGGTCAAATAAATGAAGTTAAAAAATCAATGGCATTTTTAAATTTAAATATTCCTCTATTCGGGCTGGTTAAAAATAGTAAACATGAAACTCAAAAATTAATTGATTCAAACAACAACGAAATTAAAATAGATGACCAAGATGTATTTAATTTGCTTTCAAGAATGCAAACAGAGGTTGACAGGTTTGCAAAATATAGATATAACCAAAAACAAAGAAAATCAATGTTATCTAATCCTCTATTAAAAATAAAAGGTATTGGATTTAAGACAATAGAAAAATTAATTAATTATTTTGGCAGTTATAAAAAAATTCAACAAGCTTCCTTTGATGAAATATCAAAGGTAGCAAACAAAAAAGTTGCTAAGCTAATTTGTGAAGACAAAGGGGAATCGTTTTATTAAATTATTTTTCAAAAATATTAAAAAAATTTTTAAATGGTGTAAAATAATAAAGCAACAAAATAATGGTCGTGTAGCTCAGGGGATAGAGTACGTGCCTTCTAAGCATGTGGTCGCAGGTTCGAATCCTGCCACGATCGCCATTTTTTTTATTCATTTTTTTAAAAAAATAAAAAATATGTATATAATTATTAACGCATACAATTAAATACGCGAGTGTAGTTTAATGGTAGAACATCAGCCTTCCAAGCTGAATACGTGGGTCCGATTCCCATCACTTGCACCATTGCGGATGTAGTTCAATGGTAGAACATCAGGTTGCCAATCTGAATACGTGGGTCCGATTCCCATCATCCGCACCATTTGAGTAAAGTTCACCAAAATTGACATAGGCTAAGGCTTGTGTCAATTTTTATTTATAAAATCGCGTGAATACTGTTACTGCTATATTTCATAAAACCTTAGGTCTGGCAGTTATTATGGTTAATAAACATCGGCTCTTTTTTTTATTATTTTCTTTATTTTTTATTAAGTAATAAGGTGCAATAAAAAATTCAAATTAAAAATTAAAGTTTGAAATATGAATAAAAAAAGTATGCAAAGACGTACTAAAACCATTATGATATTATTTTTATTTTATATTGAAATAAGCATTTAATTACGAGTTTAAATTTTTGCTCTATTTTAAGGCTATACATCTTTTTTGTTTTTAAAACATATTGATGCTTAATTTATTATAATTAACTTAATATTTAGCAAATACGTTAATTGACTAAATATTAACCTTAAAAATATAAAGGAAAATTATGGCAAAAGTATTAATAATATATGGTTCAGTTATACCAAGAGAAAATTCAATTTCAACAAAAATGTTAGATCTTTTTGTTGATGAATATAAAAGAAATAATATTAACGATGAAATAAAAATTCTTGATTTAAATAATGAAGATATGTATTCATTAACTGACAAGAACTTCAAATTGTTTTATAGTGAAGAAAACTCTGACAAATATATAAATTTATTGAAGTCTGTTGATAAAGTTGTTATGAATGCGCCGATGATAAACTTCAATATTCCAACTATAATCAAGGCATTTTTTGACCGTATTGCAGTTGCAAATAAAACATTTTCATATAAATACGCAAGCAAAGGTTCTTCAATTGGTTTATTGAATAATTTAAAAGTTCAAATAATTGCAACGCAAGGAGCTCCATTAGGTTGATATCCATGAGCAAGTCATATTAATTATCTTGAAGGAATGTGAAAGTTTTTAGGCGCAAATCTTTCAGAAAGCATTTTATTAGCTGGCACAAAAGTTGAACCTTTAAATAAAATGCAACCTCAAGAAATAATTGAAACTATTAGCAAAGAAATTATTAAAAAAGCAAGCATATTTTAATTATTTTTTGTTATAATGTAAATGCAATTTTTATTGCCACATTAAAGTGGAAGAATTTGAAACAAATTCGTTTGAACCACTATAATCGAAAGGATTTTATTTATGGCTAAAAAAGTAGTCAAGAAAGCAAAATTACAATTTGCTGCTGGCCAAGCAAAACCAGGCCCAGCATTGGCTGGTGTTGGTATTAACATGCCAGAATTTACAAAGGCATTTAATGACAAAACAAGAGAAAGAGGAAATGAACCAGTTCCTGTTTTAATTACTGTTTATGAAGATAAATCATTTGAATTTAAATTATTTACTTCTCCAACATCATACAAGTTAATCGAAGCTGCCAAAATTAAAAAAGGTAGCGGAGTTGCAAACAAAGAAAAAGTTGGTTCTATAACAATTGATCAATTAAGAGCAATTGCAGAATATAAATTACCAGATTTAAATACAACTAAAATTGATTCAGCAATGAAACAAGTAGCTGGTACTGCAAAGAATATGGGAATTACAGTTGAAGGTTATGACGAATGACTAAAAGGAAGTGCTAACTAATGGCTAAAAGAATTACAAAAAACATTAAAAATGTTAAAAGCTTAGTTAATAAAAAAGACGTATTTCCTCTTGCAGATGCTATTGAATTAGCAAAAAAAGCTTCATTTGCAAAATTTGATGAATCTGTTGATATTGCAATTAGATTAAATCTTGATACAAGAAAATCAGATCAACAATTACGTGGATCAGTTGTACTTCCACACGGTACAGGAAAATCAGTTAAAGTTTTAGTAGCAACTGACGAAGTTTCAGCCCAAAAAGCTTCATTAGAAGCTGGAGCAGATTTTGTTTACAGTTCAGCTGAATTACCAGAAGTATTAAATCAAGATAAATACGATTTTGATGTAATAGTTGCCGATCCTAAAATGATGTTAGTTTTAGGAAAATTTGGTAAAAAATTAGGACCTAAAGGTTTAATGCCAAACCCAAAAACAGGAACAGTTACAATCAATCCTGCTAAAGCTGTTGAAGAACTTAAAAAAGGTAAAGCAAATTATCGTGCTGATAAGGGTGGAATTGTTCACGCCTCATTTGGTAAGAAATCAATGGAAACAAATGTTCTAGCAGAAAATGCTGATACTATTATTCAAACAATTAAGAGATTAAAACCTCAAACAGTTAAAGGTACATATGTTTTAAATATTACCGTTTCAACTTCAATGGGTCAATCTGTTAAAGTAAAAATTGATTAATATGTTGAAAAAAAGCAGTCTTAACACTGCTTTTTTATTTGTTTTTAATAAAAAAACAAAATTGTTAAAATAAAAAATACCTCAAAATTAGAGGTATTTTTTAAATTTAGTTAATTTTTTAGATTATAAAATGAGTTTTTGCCTTCGAATTTAGCTTCATTGCCTAATTCTGATTCTATTTTTAACAAGCGATTGTATTTAGCAATTCTATCTGTTCTTGAAAGAGAGCCGGTTTTGATTTCTCCAGTATTTAATGCTACTGCCAAATCTGCAATAAATGTATCTTCAGTTTCACCTGAACGGTGAGAAATTATTGCTACAAAGCCAGCTTTTTGTGACATTGTTATTGCCTTAAATGTTTCAGTTAATGAACCTATTTGATTTATCTTTATTAAAGATGCATTGATTGCATGCTTGTCAATAGCCATTTGAATGTATTTTGGATTTGTTACAATTAAGTCATCTCCAACTAGTTGAACTTTGTTGCCAAATCTCTTATTCATTTTTATAAAGCCATCTCAATCGTTTTCATCATGTGAATCTTCAATAGAAATTATTGGGTATTTTTCAATTAAATGGCCATAATATTCAACAAATTCATCGCTAGTAAATGAATATTTAACATTTTGATATTTTTCAAATCCTGGGCGTTTTTCTTCAATTGCTTTCTTGAATTTTTTAAATACATATAACTTTGTATCTGAATGATAAAGTTCTGAACTTGCAGCATCCAAACAAATTGCTACTGCTTTTTCTCCTGAAGTGGCTGGATTATATCCTGCGACTTTAATTGCATTTACTAAAAAGTCCAATGCTTCTTCATGAGTGTGTAGGTTTGGTGCAAATCCACCTTCATCTCCAACTTGTGTTCCATGACCTGCTTGTTTCAATAATTTTGCTAATGTATGAAATACAAAGTTTGCCATTTGCAATGATTCTTTAAATGTTTTTGCCCCAATAGGCATTATCATAAATTCTTGGAAGTCAATAGTATTTGAAGCATGTTCTCCACCATTAATAACGTTTAGCATTGGCACTGGCATTTTGTATGCTACTTGGTTGTTTAATTTTGCCAAATATTTATATAATGGAAGATTTGCTTCAATGGCGCCAGCCTTTGCAATTGCTAATGAAACAGCTAATATTGCATTTGCTCCTAATTTTGATTTTGTTTCAGTTCCATCAAGATTAATCATTAAATTATCTAATTCTTCTTGCTTAGAAACTTCCTTGCCAATAATAGCTTTAGCAATAATATTATTAACATTATCGCATGCTAATTGCACACCTTTACCGCCAAATCAATTATTTTCGTATTTTGTTCCTTTATCGCGTAATTCAAGTGCTTCTTTTGATCCTGTTGAAGCTCCTGATGGTACTAATGCTTCAGAATATGCAAGTTCAGTATTTAATTCAACTTTAACTGTTGGATTTCCTCTACTATCTAAAACTTCATAGGCATTAATCTTAATAATTTTTGACATAATTCTCCTATATGATTATTTAAAATAAAAGCTAAAAATAAATGAACATCATTTATGTTAGTTTACATAACAATTATATTATTAATTTTTAAAGGTAAAAAAATAATTGTTATTTTGTGCGTTCAATCTAAAAGAAATATAATTTTAAAAATATTTTTATGTAATGAAAAATTGCGATTTTAAATTAGTGTAAAATTTTATTTATGAATAACATTGATAATAATTTATTTTTTATTGAAGAAGAAATTAAAAAAATTGAAAAAATTGCAAAAGAAGACCCACTTAAAGCAATTTATATTTTAGATGAAGACAAAAAACAATTCTCTCTAAAAGAAGACTTGGATGATTTAGATGCATTGAGGGATAACATTGAGTTTCAACTTAAGAAACAAAATTTAATAACCTCAACAAAACTTGATACATTAAGTTTAATAAACAGTTTAAAAAACAAAAAAATGGGTTATGCATTTATGCTGAGCTACAACGAACTTAAAAAACGAGATGATATAGCAAAATATGCAATTGAATTTCAAGATTTTTTTAAAGGAAATGATTTTGATAGTAAAGGATTTCAAACATTGATTTATGACTTATTAGTCAATAGTAATATTGATTTTGAATACGATATTCAAAATAAAAAAATTAATCCCAAAAAATTAGGATCATTATTAAAAAATGCCGAAATTCAAAAGATGCAAGATGAAATTATTGCAACTTTTGACAAAGATATTTCAAAAAACAAGGTTGCAAGGCAAGTGTTTTCAGCGTATTTATTTCAAAATTGGGTTGCAATCTTATTGCATGAAACAAAAGACGATTATTTAAAAATATCACACGTAATTGAAGTAATTTTAGGTAATCAAACGTCAGATTCACTTTCTGAAGAAGAAAAAGAGTTATATAACATTTTTAAATAAATTATTATGTATAATTTATAACATTAAACTAAAGGAGCATTTATGTCAAGAACATTAAATAAAAAAGAATCAGAATTAACTATTGAATATACAATTCAAGGTGAACAATGAGAAAATGCTAAAGAAAAAGCAAGAAAAGCATTAAGAGCCAATGTTCAAATCGATGGTTTTAGAAAAGGCCACGTTCCCGCTTTTGAAGCTGATAAAAGAATAAATCCAATTGAAGTTTATCAAAAAGCATTGGATAGTATTTTAGATAATATTTATAAAGAACAAATAATTAGCCAAATAACCAAAGATGATGAAATTGTTGGACATGCAAATTTAAGAATTAAAGATATAAATGAAGAAAAAGCAGAAATTTCATTTGTATTTCCACTTTTCCCAGAAGTTAAATTGGGAGATTACAAAAAATTGGGTGTTAAATTAGGATCATGTGAATTAACTGAAAAAGATTTAAATGATGCAAAATCACAAATAGCTTCAAACTATGTAGTTATGCTAGATTCTAAAGAACCAATTAAAGAAAAAGATCAAGTAAACTTTAATTTCAAAGGCTTTATTGACGGAAAACCATTTGACGGCGGAGAAGCTGAAAACTTTGATTTAGTAATTGGATCTAAACAATTTATTCCAGGCTTTGAAGACAAAATGATCGGCCTAAAAGCTGGAGAAGAAAAAGATTTAGATTTAGTATTCCCAGAAGATTATCACGCTAAGAATTTAGCAGGAAAATCTGTTGTTTTCCATGTAAAAATAAATTTCGTTAAAACTCCAAATTATCCAACAATCGATGATAATTTTGTAAAGGAAATTAATTTGCCAAATGTTAAAAACGTTGCCGAATTTGAAGAATATGTAAAATTTATTGCTTTAAAGAACAAATTAGCAAATGTTAAAAATGAATTTATTAATGAAGCAGTTAAAAAATTATCAGAACAATCAGAAGTTTCTTTATCAGAATCATTAGTTGAAGAAGAAGCAGATAAATACTATCAAAATTTCCTTAACAATTTAAAACAACAACAAATTTCTGAAAAAGATTATTACGAATTTTCAAAGAGTACAAAAGAAGAATCATTAAATGTATTTAAAAAACAAGCCCAAATAAACTTAAAACAAATGTTTGTATTAGGTGCTATTGCCAAAGCTGAATCTTTAAAAGTAACATTAGAAGATTATGAAAAAGAAGTTGAAAAATTGGCAAAAACATACGGCTTAGCTCCTGAACATGTAAAACAAATTTTGAAATTTGAAAATGTTGAAAATAATTTAATTAATGAAAGAATTAGCGAAATTTTACTAAAAGAAAATGACACAAAAGCATTTGAAAACTTTGACAAACTATTAAAAGAAGTCGAAGAATATGAAGCTAAACAAACTCAAGCTATTGTAGACGAAGCTAAACGTAAAAGAGAAGAAGCAGAAAAAATAGCTAAAGAATCAAAAGAAAACATTGACGAACAAAAATAATATAATTTAAAAAGTGCCATAAAAATTGGCATTTTTTATTTATTGTTTTTAACAATCATTAAAAATAAAATAATATTGTAAAATATTAACTATGAAAATATTTGAATTTAAATCAACTGAAATGAGAATTGCAGAGGCAACCCTTAAAAGGATAAATGCTTTAGAACCAGAAATATCAAAATTAAGCAACGAGAAATTGCAACAAAAAACTATTGAGTTTAAGTCACGTTTAAAAGCAGGAGAAACGCCAGAACAAATTAGACCTGAAACGTTTGCTGTTTGTAGAGAAGCAACAAAACGTATTTTAGGCAAAAGACCATTTGATGTTCAAATGATAGGCGGAATAATTTTAGATTTAGGTTCTGTAGCAGAAATGAAAACCGGTGAAGGTAAAACTATTACTTCTATAGCACCAATTTATTTAAACGCAATTACTGGTCAATCTGTTATTGTTTCTACAGTTAATGAATATCTAGCAGAACGTGATGCTCAAGAAATGGGGCAAGTTTTTAACTTTTTAGGTTTAAGTGTTGGAATAAACAAATCTCAAATGTCAAGCGAGTTAAAAAGAGAAGCATATGCTTGCGATATTGTTTATTCTGTTCATTCTGAATTGGGTTTTGACTATTTACGTGACAATATGGCAATGAGTAAAGAAGAAAAAGTTCAACGTGGACTTGATTTTATTTTACTAGATGAAGTAGATTCAATATTGATTGATGAAGCAAAAACTCCTTTAATTATTTCAGGTGGTGATAATGACGAAAGTAATTTGTATACAATTGCAGATTTGTTTGTAAGAACATTAAGTCAAGATGATTATTTTATTGATGAAGAAACAAAATCTGTTTATCTGACAGACAAAGGAATTGAAAAAGCTAATAAATATTTCAATTTTAAAAATCTTTATGATATAGAAAATTCTGAACTTGTTCATAGAATTCAAAATGCTTTAAGAGCACATAAAGTAATGAAATTAGATGTTGAATATATTGTTAGAAACGACAAAATTGAATTAGTTGATTCCTTCACAGGTAGAATAATGGAAGGAAGAGCTTATTCAGAAGGATTGCAACAAGCAATTCAAGCAAAAGAACGTGTTGAAATTGAATCAGAAACCAAAACATTAGCAACAATTACTTATCAAAATTTTTTCCGTTTATTTAAAAAAATAAGCGGTATGACAGGAACAGCAAAAACAGAAGAAAAAGAATTTATTGATATTTATAATATGAGAGTCAATGTTGTTCCAACAAATAAACCAATAGCTAGATTTGATGATAAAGATGAAATCTATGTTGATATGCACAGCAAATGAAAGGCAGTTACCGAAGAAGTTAAACGTGTTTATAAAAAGAAACAACCAATATTAATAGGAACTGCTCAAGTTGAAGATTCTGAAATATTGCATGAATATTTGCTATCTGAAGGCATACCTCATACAGTTTTAAATGCAAAGCAAGATGCTTCAGAAGCAGAAATTATTTCTAAAGCGGGACAAGTAGGAGCAGTTACAATTGCCACAAACATGGCTGGACGTGGAACAGATATCAAGCCTTCAAAAGAAGCACTAGAATTAGGTGGATTGTATGTTTTAGGAACTGAAAAAGCTGAATCAAGAAGAATTGATAATCAACTAAAAGGACGTTCTGGCCGTCAAGGAGATGTAGGATATACAAAATTCTTTTTATCGTTAGATGACCAATTGATTTTGCGTTTTTCAGTTCAAGATCACTGAAAAGAAATTTTTAAAGAATATGGCGAAGATCCAATTCCTGGTGAAGCAATTAGAAAGGCCTTTTTGAGGGCTCAAAAAAAGATTGAAGGGTTTAACTTTGATAATAGAAAAAGCGTCCTAAATTTCGATGACGTAATTAGACAACAACGTGATTTAATTTATGAACAACGTGATCTAATTTTAAATAGAGATGATCTTGGAACAATAATTAGAAAAATGATATCAGTTGCAGCAGAAAAAACAGTAAATAACCCATTCTTTATAAAAAACAATAGTTTGGATTTAACAGAATTTGTAAAGCATCTAAATAACAATTTTATGATCTTAACAAATAAGGAATTTAGTGTTGAAGAATTAGTTGAATATGATCGTGAAGAATTAATTCAATACTTAATTGAAACTTGAAATAAATGTTATGACCAACTAAGACAAAATATCATTGATAAATACGGGATATCTTCATTAATAAATTCTGAAAGAAATATAATATTAAGCGTTTTTGATGCTGCGTGACAGGATCATATTAATATAATGGACAGATTAAGAAGAAGTACAAATTTAGTTCAATATTCTCAAAAGAACCCATATCAAGTTTACACTCAATTGGGTTCAAAGAAATTCAAAGAATTAACAGACAGAATTGCTTTGGAATGTACAATTAATTTAATGAATAATTACGACGCAATACCTTCAACAAATACTGATGTTGATTTATCATGGTTAACAAATGCTTTTGAAGAAAAAACAGATATGGCTCAAACAACTTCAAAGAACCAACAAGCTCTTGAATTTATCAATTATTTATTGAAGTCAGAGCGTGATCATTTGATTAAAGAAGGTTTAGGCGAACAAGAAGTGGATGATAAACTAAAGGAAGTGGAAAGATCAATATTAAAAGAGTTCCAATTCCAACTTGCTTCTTTGCAAACTAATGAAGAAAAAAAATAACGAGTGTTTAAAACTCGTTATTTTTTGTTATATATTCGCAATAAAATTCAACAGCCTTTTTCAATATTTCACAATCACTTTCGTATGTTATGCGATATAACAATGCATTTGTTGTATCTTGCCATTCAACTACCTTAATTTCACTATCTTGCTTAATTAAATCAAAAGATGTTGGCTTAGCAATAAAATAAACAACTTCTTTAGATATTAATTTTCCAATAATATATTTATTGACTTCTCTAAAACCCTTGAGCAATTTTATATCTATGTTAGTTTCTTCTTTTACTTCTCTAATCGCGGTTTCCTCTTCTGTTTCGTTTTTTTCAACATGGCCTTTTGGGAACCCTCAATGGCCTGCTGTTTGTTCAACTAATAAAACAAAAAGATTATCATTTTCTTCTTTGAAAACAATAGCGCCACACGACTTCTCTTTTTTCATTAGCGAAATTTCCCTTACCTTTCTTAACTACAATTTATTTATATAAATTATATAACAATAAAGAAAATGCTCGCCTTGCCAAAAACTTAATTATTCATCTTTTTAAAAATAAAACTACATAATATTAATGATGAAGCAATTGCAACTAAAAATATCGTACTTGAAATTCACATTTCAACTTTAATTACTTTGCCTTGTCAAAGTATACTTTGTAATAAATTATTATTTTTAGTCATTACTGCTTGAATGTATGTTTTTCTAATTGAAGGTAAAATTAAACCATTAACAATAAAACAAATTCCACATATCAAAATAAAAATGCTGATTATTAATAGAGTTTTTAATATCTTTTTGTTTGCAATTTCTGATTTAAATCTATATACTAAAAAGAAATTCAAAATATAATAAACAACATAAAAACCGCAAATTATTTGTCCAAAGCAAGATATTGCAAGACGGCTATTACTTGAAAGTGATTGTATTGAAAATATCCCGGTAATCAAAAAAGAAATCAAAATCGCTAAAAAGCCAAATGCGGTTGTTATTAATAATCTTTTTATATTATTTTTTATAATATTGTTAATATCCATAATGCCTCTTTTGAAATATTATAAATGATAATAATAACCTTATTTTTGAATTTGGTAAAATTAAATTATTAAAAGGAGAAAATGTGGCACTTACTAATAAATATTTAGCATTGTATAGAAAATATAGACCAAAAATTTTTGATGATATAAAAGGCCAAGATCACATTGTAACAACATTGAAAAATATTATTTTGAATAATAAATTGAGTCATGCGTATTTATTTTGTGGTCCCCATGGAAATGGAAAAACTTCAACTGCTAAAGTTTTTGCAAACACAATTAATTGTTTGCATAATCAAAGCGATATATTAAAACCGTGTGAAGAATGTATCAAAAATGTCGATAGAAATCTAGATATTATAGAAATAGATGCTGCTTCTAATACCGGCATTGATGACATTAGAGAATTAAAAGAAAAAATAAAACATTTACCAACACAAAGCAAATATAAAATTTATATTATTGATGAAGTTCATATGCTTTCAAAAGCGGCCTTTAACGCTTTATTAAAAACAATTGAAGATCCACCTAGTCACATAATTTTTATTCTAGCAACAACTGATCCTCAAAAAATACCAGAAACTATTTTGAGTAGGGTTCAAAGATTCAATTTTAAACTTATGACAATTGAAACATTAGTTTCTCAATTGAAGGATATTTTCAATAAAGAAAATATTGAATATGAAGATGATGCAATTAATGTTATAGCTAAATTAGGAAATGGTAGCCTTAGAGATTCATTGAGTATTGCAGACCATGTTGCGATATATTGTGCCAATAAAGTTATTAAGAAAGAAGTTGTTGAACAATTATTTGGAATTGTAAATATAGATAATAAAATAGAGTTATTAAATTGTATGTCAATGCATGATATCAATAAAACATTGAATCTTGCAAGAGAATTATTTAGTCAAGGTATTGATATAAACATCTTGATAAACGATTTAATAAACATTTTAAAAGAATGAATAATTTTTAATAAAACAAGAGATCAAAGTCTTGTTTCAACTGACTTTAAAATTTTATTGCAAATTAAAATTGATGATGTTAAAGCACACTATTACATTGAAACATTGATAGATCAATTGCATAGTATTGCCGTAAGTGATAGTCCAGAAGATTATTTCGAAATTCTATTGCTAAAGTTATGTAATTACTTAAGTCCAAAAGAAGAATACAAAAATTCTGCAGTAATGCCAAAAATTAGCAATGAAGAATTTAATAATTTTAATAGTCAAGAAAAACAGGCCATTAATAAAAATAATATTTACAAATCATTGGATTTATCCTCAATAGCAAATGGATTCAATGCAAACAATTCCGCAATACAAAATTCAAGTGATAATAATTCAACAGAAGACTTAGCTAATAATTCAAAAAAGATTGATGACTTATTAGAAAAAACTGCAGAAATATATTCATTAGATCAGACATCAGAGTTTGAAGTTGCTGATATTTCTAATCAAATAATAAGCAATAATCAAATTATCATAGATTCAAATGTTGATAACCAAGAAACAGCCGATTTACAAATATCAGAAAATTGAAAAGATTTAGTAAATGAGGATTTAATTTCAAAAGAAAATTCAGAAAATAAACATGTTCCTTTGGAAACAATAATCGACTGTTTATTAATAAAACAATATTTGAAAAACAATATGTTAAATGATGGCCGTAGTAATTTTACAGACATTGATGCTATTAAATATGGTATGTTAAGTCAAAAAATAACAGACAAAAATGAAATTAACATCTTATCAGACTTTAAAATTATTTTTTCAACGAATGATTTTATTTTGTTAACATGTGATATAGATGAAAAAGTCTACGATTTAAATATAAATTCACATAATGAAAATATTATGGCTGCATTGAATAAAGTTTTTGATAGATATCTTTATGCTTATGCCATTACAAAAGGCGAGATATTAGAAGCAAAGAAATATTGGAAAGAAAATATAAACGAAATAAGAGCCAAAAAAGTTAAACCTTTGGAAGATTTAAATTTAAAATACAATAAAACAGTTCAAAAAGATGTTGCTTGAGCACAAGAAATTTTTGGCGATAAGTTCAAGTTAAACAAATAAAAATTAAATAAGGAGAGAGTAGTATGAGTATGAACATGAATGAAATGCTTAAAAAAGCACGTAGAATTCAAACTGAAATGGAAAAAGATGAACAAGAAATTCAAAAGAAGGAATTTACTATTGAAAAGCAAGGCATAACCGTTGTTATGAATGGTTTAAGAAAATTAGTGTCTATAAAATTTAATCCAGCATTAATCGATCCCGATGACCCTGAATTATTAGAAGATTTATTGATGCTTGCGGTTAATGAAGCAATAGAAACAGTTGATAAGGCTTTTGATGAAATTAATGCTAAATATTCTGATAGTGGCCTTTCATTCTAATGAGCAATATTGAAATACAAAAACTAGAAGAACTTTTAAAAACGGTTTTTGATTTTACTAAAAAACAATCTCAAAAATTTATATTCAACATTTTTAAATCATCAATTGAAGATATAGACCAAGTTTGCAATCAAATAAAAGCATTGAGAAATTCAATAAAAAAGTGCAACATTTGTAATAGATATAACGAAAATACAATTTGCAATATTTGCTTGGATACTAATCGAACAAAACAACTTATGTTAGTTGGTAATTCGTTAGATATTGATAAATTTGAAAATATTGGTTTGTATAAAGGAAAATATTTTGTATTTGATCAAAATATAAATTCAAAGAATGAAGATTCTATAGTTTCAAGTTTATTAGAAAAATTAATAGATATATTAAAAGATAAGAATGAACTAATTCTTTCACTTTCCCCAACTATTGAGGGCCAATTTAATATGCATTATATTAAAAAGTTCTTGAAAAACAAAATAAATTTTACAAATATTTTTCAATTATCAACAGGTATTCCTTTAGGTTCATCAGTTGAATATATTGATCAAGATACATTAAAAGAATCATTATTGAATAAAATTAAAATATAGTAGGAGCATTTATGTCAAAAGGAAAATTTGTAGTATTTGAAGGGATGGATGGTTCAGGAAAAACAACTATTATAAAGAGATTAAAAGAATATTTAGAGTCTGAAAATAGACTAGATGATTTCGTATTTACAAGAGAACCAGGAAGCTCACATTCAAAAGAAGCAGAAGAAATAAGACAGCTAATTTTAGATAATGATAATTCTTTTAGTGTTATGGTGGATGCTTTGTTATTTGCAGCAAGTAGAAGGTTAAATCTTGAAAAATGCGTTTGGCCAGGATTGGAAAAGAATAAAACGGTTATTTCAGATAGGTATTGAACCTCATCATTTGTTTATCAAGGAGTTTTAGGCGGTTTAGGACTTAAGAAAGTAAAAGACATCAACAATATTGCAACTGAAGAAACAACACCTGATTTAACTATATTCTTTGATATGGAGCCAGAACTATCAGTTGAAAGAATTACGCAAATTCGAAAAACAAAAACTGATAGATTAGAATCTACCAATGTTGAATATTACAAAAAACTAAGAGATTCTTATTGATATGTTATTAATCAAGGTAATGAAAAATATGAAATTATAAAATCTGATTGTAATATTGATGAATTATTTAAAAGAGTATTAAATGTATTAAAAGAAAATAAAATAATTTAATTTGATTTTGTCTTTTTATATTTAAGGTTGTAAAAATATGATAAACAAAAATTTGAGTAAAGTATTGGAAAATTCAATAAAAAAAAATAAGCTTCAACAAGTTTATTTACTATTTTCACGTGCAAATCAAGATATTGATTTAGATATTTTAGAATTTATTAGATTAGTTAATGATGAAAAACAAATAAGCTGACAACAATGTTTGCAAAAAGAATATTGTTTTATTATTGATGTAAGCATAGATGAAATATCGAAAGAAAATTTACAAGAATTTTTAAATCGTGTTTCCAAAAGCTCTCTATTTGCTAATAAATATAAATTTTTAATTATTAAGTGTGTTGAAAATATTACACTAAAGGCCGCAAATAGTATTTTGAAATTTTTAGAAAATCCTCCATTTAATACAATAATATTATTGCATACAATAAAGCCTCAAAACGTTTTGAAAACTATCAAATCAAGATGTTTTATTATTGATATTCGTGATGATTTTAATGCTGTAGCTAGCGTTGAAAATTTTTTTATTACATATAAAATTGACGTAAGCAAGCAAAAACAATTTAGGGAACTTGCTGATTTAATATACAATTCACCAGCAAATCTATTTAATCTTATTTTGTTTTATGTTCAACAATTAAATATAGAAAATTATAAAAATATTATTCATTTTTCTATTTTTATTTTTAAACAAATTTACGAATTAAAGAATAGCATAAAAAAAGATAAAAATATTAGTAAATTTGATTCTATTTCAATATTAAATATTATTAATAATTTATATAATTTTGTTGAATTAATTGATAACAATAATTGTATTTTCAATATTCAAAAAGCAAGGCTAATTTTAAAATTAAAGGAAGAATATGAATAATAAAATTTATATAGTTGGAACTCCAATTGGAAATTTAGAAGATATAACATATAGAGCAATTAAGGTTTTACAATCCAGCGATATAATTTTGTGTGAAGATATAAGAGTTAGTCAAAAATTATTGCAACATTACGAAATTAATAACAAACGTTTGATTGCATACCATAAATTTAATGAAAAAGAAATGACACCTAAAATTATTGAATTATTGCAACAAGGGATGATTATTTCATTGATTTCTGATGCCGGTATGCCTTGCATTTCTGATCCTGGTTTTGAAGTTATTAAAGCAGCCAAGGCAGCAAATATTGGCATTGATGTAATTGGCGGACCAACTGCTCTAATACATGCAATAATAAAGGCTAATTTAGACAATTGCTTCACATTTCTAGGCTTTTTAAAAGATAAGTCGGGTGCAAGACAAAATCAATTAAAATCCTTGACATTTGGCACATATGTTGCTTACGTTTCACCTCATAAATTGCTTTCAACTATTAAAGATTTTGAGATTATTTTTCAAGAGCAAGTTAAATTATTTTTAATTAAAGAAATGACAAAAATGTTTGAAACCTCATTTGAAGGAACACCTCAAGAAGTTCTAACACAACTAAGCGAAGAAGATCAACGCGGTGAATATACTCTTGTATTTAGTTTCAAAGAACCAAAGAAACTTAAAATTAACAAATATAAAAAAGAAATAAAATAAAAAAGTTCGGACGCGAACTTTTTATTATTTAATTTTTTAAATTTAGTTGTTATTTTTAGCAATAAATGCATCTAAACGGCTAGCTTTTCTAGAACCATTGTTTTGATGTAAAACGCCCTTTGAAACAGCTTTGTCAATTTCGTGGTGAGCATTAGCTTGAAATTCAGCAGCTTTAGGATCTTTATTAACAGCAGCAATTTTTGCTTTCTTAATAGCTGTTTTAACTGATGATTTAACGGCTGAATTACGGATGTTTGCTTTTTCGTTAGATAATATTGCTTTTTGTTTTGATTTAATGTTTGCCATTTTTTGCCTTTCATGCTTAATAATTAGCAATAGTATTTTACAATAAAAAAGCGTTTTATTTAGAAAAATAATATTTTTATTAAAATATTCTATATGGTAACAAAGAAAAAATATGATGGATATCTTAAATATTCAAAAAATAATGAATCTGAGTTGATAAATTATTATCTAACTAATAATAAAAATATCTATTTAAAGTTAAACAAGGAACAAGAAATCGTAGCCTATGTGCCACGTAAGTATATGAATACAATAATATTGGATAATTTTTTAAAATCGTCGGTTCCTAAACTTCTAGAAATAAAAACAAAAATTGAGCAAAAGAAAATACTTGCAATTGATATAGAAAAGAATAAATTTTATTTATGAGGCAAGGAATCAAAATTTTTAATTCAAAAAAATATGTTAAATTTATTTGAATTAAATAAGAATTTAAAAATCTTAAAACAAGGTATAGAATTTACTCTAAATAAATATTTAAAAAAAATACTGCTGGAATTATCAACTTCTTATGTAGAAAAATTTTGTAAGTTATTATGCATACCATCTGAGTCAATAAAAGTAAGAATTTTGAAGAAGAATAATGCCTGGGGAACAAATCATTTTTTAAAAAGAACAATAACTTTTTCAGATCGGTTAATTTTTTACAGAAAAGAAGTTTTGGAATACGTTGCTTGCCATGAAGTTGTTCATTTTATTTATCAAAACCATTCATCTGAATTTTGAAATATGGTAAAAAAATATATTCCTAACTTTAAACAAATAAAAAAGGAATTAAAAGATAAAATTTTTATATAAGCAGGAGATTTCATGGAAAATAAAACAAAATTTATAAATGGAATTATTATAGGATTAGTTTTGCTATTAGTAATAGTTATAATTCTAATTTCAGTTTCAATTGTTCTTTCTAAAAAGAATAGAAGATTTTTAAAAGATCAAAAGGTATTTTTAGATTCATACAATACACAATTAAAAAATATTAGTGAATCTATAAAAAATTGCAAATTTATAGGTGCCAACGATTATTCTTATAACGATCAAAGAATACACTTAGATGATATATTTGTTACTGATAAAATTATTTTTGTATTAAAGCCTTATATGTATGACTCTGACTCTTCTATTCAAGGTGACTATTTGAGCAACGAATGAATTTTAACTAAATCAAATGGTAAAAAATCTAAGATACCCAATCAATTAATTAGTTTAAAAAAATCTATGAATAATTTTGGACTATTGTTTTCTAATGAAATGAGTATATGTTCAATTTTTGTGCATAATTTAAGCACGGAATTAAATATAGAAGATGTGCCATCGTTTCAAGGAATTTGCAATATTAAAGATTTATGTGCTTCAATAAAATCTGTATATAATTCGAATATTAATTCAATGTATAACCAATCTATTTCAACTATTTTCAATGTTTTAAATAACAAAATTGCAAAATAATTGTAAAAATTTAGTATTTTCAATAATTTTTCAATAAAACAGCAATATCTTTTTGCTATTTTAGTGTTTTTGTGTAATAATTTAATTGTAAATATTAATATTTAATTTATTTATAAAATTTTTTAAAAAAATAACCTTACGTAAGGAGTATCTTAAATATGGCAAAAGAACAAAAAACAGTATTAGAAAAATACTATATTTCTGAAGCTTTTGATAAAGATAGAAATATTTCATTTAACTTTAAAAAGGCAAATAAGGGCATTTCAGGAAACTTTTTAAATTTTTCTAGCGCAGTTGAAGAATTTATTAAAGTTAGTGAAAATACACAAAACCCAACCAAATGTTGATTCCACCGTGATGGCGCTTACCGTGGATGCGTAAACATTGATGGTGCCCGTGTTATTGTTGAAAAAGTTGCTGAAAAACAAGTTAAAAATGAAGAAGCTATTGAATTCATCGAAAGAGAACAATTGGTTGAAAAACCTGCAGCAAAAGCTAAGAAAGTTGAAGAACCAAAACAAGAATGTGAATGCTCATGCTCTTGTGATTGTTTAACCGGTATTGAAAACAACAAACACTGAAAAGAATATTGAACAAAGAAATCTTCAAATAATATTGTTTGTTCAGGTATACAAGACCACAAAGGTGGAGAAAAACTATTTATTGGTCTAACAGTGTGTCTATCGCTTCTAACAATTATTAATTTGATTTTGATTATTTTGTTAGCAACAAATGTTATTTAATCCAATAAATTAAAAAATAAAAACTGGAATTCCAGTTTTTATTTTTTAATTTATTTTAAATTCTTTTTTAATTGTTTTAAGTAATTTACAAATTCATCTTTGCTGTATGTTTTAGAATCATCTTTTCCATATTCTCTAACTGATAAGGTATTTGTTTCAATTTCTTTTTTTCCAATTACTACTATAAATTTGGTCTTTAAAATTTGAGCATCACGAATCTTTTTATTTATTCTTTCTGGTCTTATATCAATATTAACATTAAAATCTTCATCTAAAAATTCGTTATATAGGTCTTGAGCTTTTTCATTTAATTCATCATTAATAGGAATAATTGTTATTTGTCTTGGACTTAATCAAAATGGCAATACACCTTTTGTTTGTTCAAGTAGTGTTGCAATAAATCTTTCATAAGTTCCAATTAAACCTCTATGAATTAATACTGGTGTTACAAATTGTTCATTACTATCAACAAATTTCATTTCAAATCTTGATGGTAATAAAAAGTCTAATTGTAAGGTTGACATAGTAATAATTCTATTTAGTGCAGTTTTTACTTGAAAATCAACCTTAGGTCCATAAAATGCAGCTTCTCCAATAAATTCTTTATATTTAATATTTAATTCACCTAAAACTTCACGCAAGTCATTTTCTGCTTTATTTCACATTTCATCATTTTGGAAGAATTTTTGAGTATCTTGTGGATCTCTTAATGATAAAGAAATATGATCTATTTCAATATTAAAATCTTTTAATGCTTGCAATATCATTTTATACAAGTGCTTAAATTCTGATTTTATTTGATCTGGTCTAACAAAAATGTGGCCTTCTGTTAAATCCATTCCTCTAACTCTTTCAAGTCCACTTAAAGCACCCGATGCTTCGTAACGATATAACCTTGATTGTTCTGAATATCTAATTGGCAAGTCTTTGTATGAACGTCTTGTTGAATTGAATAATATTATATGGTGTGGGCAAGTCATAGGTCTAGCAACTAATGTTTCGTTATCCATTTTAATTGGGGCAAACATAGTATCTTGGTAATGTGCTCAGTGTCCACTTATTTCATAAAGCTTCTTTTCACCAAAGTGTGGAGTTAGTACTTCTCTAAAACCAAATTTTTTATCAAGTTTTAAAACATAATCTCTAATTGCATTATGAATCTTCATTCCGTCTTCTAATCAAAATGGGAATCCTTGTCCACCTAATTGGTTAAAAGTGAATAAATTTAAATCTCTTCCTATTTTACGATGATCTCTTTCTTTTCTTTCCCTTAAGATATCTAAATATTTTGCTAATTCATCTTTTGTTTCCCAACAAGTACCATAGATTCTTGTTAACATTTTGTTTTTAGAATCACCTCTTCAATAAGCACCGGCAATTCCTAACAATTTGAAGTATTTAATTTCTCTAGAATTTTCAATATGGCCACCTGCACATAAGTCTGTAAATAGAATTTCTTTTGTTTTTGGATTTCAATATTGAAAGAATGTTATTTCTTTTCCTTCTTTTTTAAATTCTTCATAAAGTTCTTTTTTATATGGTTGATTTTCAAAAGAATATTGTTCAATGCCAACTTTTCGCATTTCGTAACCTTCTTTTGCTAATTCTTTCATTACTTTTTCAATATTTGGAAGATCTGATTCTAAAATTGGATTTTCAAATTCAAAGTCGTAATAGAACCCTTCTTCAATGGCTGGTCCAATTGCCAATTTAGCATTTGGATAAAGTTTTAATATAGCAGCCGCTAATAAATGGCTTGCTGAATGATTTAATTTTTTATTTGCTTCCATTATCTTAATCCTATTCCTTTTAATAACATATCTAAGTTAATTTGGGCAACTTCACTTGCCTTTTTTGCTCCCTCTTTACTCAATGTTTCAATTTGATTTAAGGCATTTTTATACTTATCTTGGATCTTAATTAAAAATTCCTTTACACATTGAGCGACAGCGTTTTTAAATTCACCGTAATTGCTGTTTTTAAATTTTTCAGCGCTTTCTTGTAATGAAATATCATTTAAAGCACTATATATAGTCAAAAGGTTTTTTATGCCCGGCTTTTCATCTGACAAGTATATTTTTCCTTCTGAATCTGTAACAGCTTTTAATATTTTTTTGTATGCATCTTCTGGATCATCTAATAAATAAATTGCTGATTTAGGATTTGTATCAGATTTTGACATTTTCTTACTTGGGTCAGTTAAAGACATTATTTTGGCCCCAACTTCTGGTATATATGGTTCAGGTATTTTGTACGTTGTATTGAATTTATTATTTAATCTAATTGCTAAATTTCTAGTCAATTCAACATGTTGCTTTTGGTCGATGCCAACAGGAATAATATCTGCATTATATAGTAATATATCGCCAACCATTAAAGTAGGATACATTAAAATTCCTGTTGGAATTGTTTCCATACCATTTGCAGTTTTTATTTTAGCTGATTTATCTTTAAATTGCGTCATTCTTGACAATTCTCCGATTGTCGTATTTGTCAAAATTAATCAATTCATTAATCCGTGTTGAATGACATCTGATTGAAAAAATAACACACTCTTTTTAGGGTCAATTCCACATGCAATATAAAGTGCAAAAATATCCTTTTTATTTTTTGATAATTGCTTAGGATCAATTGGCAACGTTAGAGCATGTAAATCTGCTATAAAAATGTAATTGTTATATTCTTCTTGCAATTTAACCATATTTTTTATAGCGCCAATATAATTTGCTAGCGTTAATTTACCAGTTGCAGTAATTCCACTTAATAACGTCTTCTTTTCAGAATTTTGCATAATTAGCCTTTCTTTTATTGTTTAATACAAATAAAATTTTATAACTTTCTAAGATTATAAAATAATTTTATTTTACATTTATAAATTAAATAATATTAATTAATATTAATATATTTTTATTAAAATATAATTATATTGCTTGCATAAGTTACATAGGCAAAACTATAATAGGAGAAACAAATGCTAAACAAAAATAAATTTTTATACATATTTTTAATAATCATTACTCTTGGGTTAATCTTAATATTTTGAAGAAAAAAATACAAGGTTTTAAAAACTAAGAATTATTTAACAACAGACAATGAAATAACCTTTTCAATGCAAGATTTAATTTTATATTTGGGTGGAATTTCAAATATAGTTTCTTCATCAGCGACTCAAAAAATTGTAAGAATTTCATTTAAAAATAAAGCAAATATTTCTATTGAAAAAATAAAAAAACTTAATGGCATATCAGGTATTGCATTTCAATCTTCAAATATATCATTAGTTGTTGGAAATTGCGCAAATTATTTATCTACATTACTTAATAAGGAATTAAACAATCATGAATAACAAAGATTCTAAATATCAAACATTAATGGCTAATTTATTGAATATTGATACATATGATACTTCACTATATACATCTATAAATAGTGAAAATTTTTTTGATATTTATAACAATTTTGGCCAAGAAATATTTGATCAAATTTTCAAAAATTACAAATTTAATTGTATTTTAACCGAAGTCAAAAATAAAGAAATGCGCCAATTGCTAGAAAAGGCACAAAATAAGCAGGAAATAATAACAATTTATAAAGATAATAACGTCAGAATCGAACAATCGATATTCAAGATGCTATCAACTGATTTTGAAAAGGCTAAAACTTTGTTAATTACTAAATTAGAATCAGAATTTCAAAAATCAATAGTAAATTGAAAAAAAATAATAAATAAAGCTGAAAATATAAACATTGAAACAAATATCTGACCCTTGCATGTAGGTTTTTTATTTATTTCAATAAAAACGGATAAAAAGACAATCTTTGCTCCCTTATTATTTAAAGAAGTAACATTAGAAGTTAAAAATTCCTTGGTTTATTTGAAATCAAATTCAGATGTAAGAATAAATAGTAAGTTAGTAACATTTTTATCTCAAGAAGGTTTTTTGTTAAATATTGATAATTTAGATTTTTCTAATTTGTCAATTGAATCTATATTTGAGCATTTAAAAAGAATTTGATCTCCTATATACAATATGCCTCAATCACTAAGAAGCAATATTCCGCTTGTATCTCAAAGTGATATTCAAAACGTATCTATCGAATTTCATCCCGGAATGATTTTTGGATTTTATAACGTTTCTTCTGGATATTTATGAAATCAAATGAAAAAAATTATTGAAAACGATGAATTTGAAAGTATTTTAGTACCCGAAATTAATAAAAATGTTTATAGAGAAAAAATTAATACAAGTATTTTTAATAATAAATTTAAGTTATTTAAGGTACAAAAAACAAACTTTTCACAAGATGTTGCCACAGTGTCTGCACTATGTCAAGATACAATAATTTGAGGACCTCCAGGTACCGGGAAATCTCAAACTATTTCTAATATTATTGTCAATATAATTGCAAGGGGATATACTGCGCTAGTTGTGTCTCAAAAGAAGGCCGCCCTTGATGTATTGAAAAATAGACTAGGACAATTGAGCTTATTTTGTTTATTTGCTTTAAATGATAAAAATTTAAGACAAGAGGTATTTTACGAACCATTAAAAAAATTTATAGAAAAAATTGAGAATTTTTCTATTTCTCAAGTTGAAAAAGGAATTCAAATTTTTTCTGATGAAGATAAAGGTTATGTTGATACATTGAATGAAATAGAAGCACTGCCTAACTTAAATAACTTACTTCATTTCTATGCCTCAACATTGAATGCCAATTTAACAATAAATGATTTTAATACCCTAAGATTATTGGCAAAAGATATAAAATATGATGTTGAAGGACATACTTTTAATGATAAGAAAGAACTTAAAAAATATCTATATGAAGTAAATTTAAAAAGAAGACCGTCAATATTTTCAATTTATCCAAGGTCAATAAAAGAAGCTAGTGAATTAATTATTAATGCGCCTGAATTATTTAAAATCAATGTAGATGAAGCAGTAAAATACATTGATAAAGTAGAGTTTGCCGAATTGGAAACTTTTGTTGAAAAATATAAAAATTTATTGATTAAAAAAACAATAAATGTAAGTAACTCAGCCGTTTTATCTAATATGTTATTTGCTAAATTAATAAATACAATTTCGAATTTCAATGATGAACAAAAACGTGAATATACATCATTTGCAATGTCTATAAGAACGGGTAGCTTAAAACCTTACATGTTTTTCCATAGATATAAAAACATAATTAAAGTATTATTTCCAATAATTGTAACTACTCCTGAATTAGATTTGTCAATGTGAGGTAAAGAAGAATTTGATTATGCAATCTTAGATGAATCAAGCCAAATCTTTATTGAAAAAGGAATTCCAATTTTATACTTAGCTAAAAGAAAGATACTTGCTGGAGATTCAAAGCAAATGCAACCAACACGTTGATTTTCGGTTTCATATAATTTTGAAGATGAAGAAGAATTGGGTGATATTGAATCTCTTCTAGATTACGCTACTGCTAGAGGAACTTATTCAATATTGCTTGATAAGAACTATCGTTCAAAGAGGGCATCATTAATGACATTCAGTTCTAAACATTTTTATGATTCCAAATTAGATGTAATAGATGATTTTAAATTAGCATTTTCTAGTGAAAGACCAATTGATGTAATTCAAGTTGATGGCGAATGAAATAATAGTTACAATGAAGCTGAAGCTAAAGAAGTATTAAGAATAGCAAAAGAAAATCTAATAAAATATGAAAAAATAATTATTCTTGTTTTTAATGTTAAACAACAAGAATATTTGACAAATGCAATATTTAGCGTTGAACCAGAACTAGAAAAAGCCTTATTTTCTGATAAAATAACAATAAAAAATATTGAAAACATTCAAGGTGACGAAGCTGATTTAGTCATTATGTCTGTTGTTTATGACAAAAAAACAGCATTGTTTGGAACATACGTTGCAAGGAAGGGCGGAAAAAACGCCCTAAATGTTGCCATATCAAGAGCAAGAGAAAAAATTATTGTTGTAAAATCAATTTATGCAGATGATGTTGAAATTAATGAACGTTCAACATCTGATATGCGTTTATTCAAAGAATGGCTAAAATTTTTAGATTTATCAATAATTGAACAAAAAAACTATTTATTTTTAGATAATGAAAATAAAAAATATGATGAAAAAACACAAACAATATTGTTACCAAAAGTAAGCAACCTTCAAATGGATGTATTGGCAAAATTAAACGAATTATTGAAGGAACTAAATGCTAACAATTTCGAAATTATTTCGAATTATTCAATAGGAACTAAATTATTAGACTTAGTCTTAATCAATAAAATTTCAAATAATATGATTATTGGTATCAATATTGACGATTTTAAATATTTGCCTGATTATAGAAAATATTTAAAATTTAAAGACGATTTTAATTTTTTACTATCAAAATCATATCCAATAATTAATATTTCTAGAATAAGTTGAATAGTAGAAAACAAACAAATTTTAAACAAGATTAAAATTTTGATTAAACACGAAGAAGAGTTAAATTCTCAAATTTTATTGTCCGAAGAAAATAACAATTTAAATGTAGAAGAAATTAAAGAAAACATTATAGAAAATAATGCCTTAAAAAACATAAAATCAAGCCAAATTAAACAAGGTCCTGAATTAAAAGAAATAGATTTTGAAAATGTTGATGACGAAACTTTTGAAGTTGATCAAGATTCTAATAATAAAATTTATGAATTAAATGAATCTGAGGTTGCTCAATTTGAAGAATTCCATAATAAGAATATAAATCATGAATTTAATGACCAAACCGAATCAAGAGCCGAATACTCAAAGGACTATGAAAAAGATTTTTTGGTTAATTATAATGATGTTGAATTGGATAATACGCATATTGATCAGGAATTTCACATTGAAAATGAATTTGAATCAACAATAAATAATAGTAATGTCATAAAGATTGAAGATGTCACTTCATCATCGGCTAACGATGAATTAAAAGTAATTAATTCTAATGAACAAAAAACTTCTGCAAAAACAGCAAGTGAAGATATTGAATCGGGATTTTCAGTTGAAGATATAAACAAAGAAAATATTAGCAATTTATTTTCTAATACATTTGATGATCTTGATATAAATATAGCACCTGAAAGTGAACAACAAGTTGAAGCTAAACTCAATTCGGATTCAGACATTATTCAAGAAAATTTTCATGAGCAAAACGATGAATATTTTAATTTAGAAGAAAATAATATTGAAGAGCAACCAAAAAATAATGATGTTCAAGGCGCAGATGAGACATTAAAATCTCAATTAGTTTATATTGACAATAATAAAGAAGTAAATGATAAACAAAAAAGCTATGATTTTGCAGGTTTATTTGCTGATGAAAACGAAGAAACTTCTACAGATGTAAAAAACATGATAGACATGAATACTGCAGAATATAATCAATATATGAAAAATAAAGATATTGAGCAAGATGAACAAAATAAAGAGATAACTAAAAATACTGCTGATGAAGAAATATTGAATTTTGATTTAATCGAAGATGAAAAAACTACGGAATTAAACGTGGAGGACAACAATGTCAAAAAATAGCGACCCTCTTGATCAATGAGAGCAAGAAATAAAAAGAAGAGTTTCAATAGAAGATCAATTATTGCATAAGGATATTGGAACTAAAATTGCAAAGCAAAAAAAGATTAAAAAAATTTTGTTCATAACTTTTTCTGTTGCTGGTATTGTTACAATCGTAGGGGCAACAATTGGAGTTACAAGCAGAAAAAAAATGGAATTAGTAACTTATGATGATTATATTGATGTTTCATTATATTCGCAAATAAATTCTAAATTTAAAGAATTGTATCTTGATGGAAAACAACCTGACAAAAAAAATTACCCAATTACTTGAAATGTAAACACTAAACCAATTTTAGGTGAGCCCTTTGAAATTCTAAAAAAAATATTAAGTGAGGGTGCTAATTTAGACAATGAAATTTTTAAAGAATTCAATTTTAAATATGATTCTAGTCAATGAAAACCTAGATTAACTAATTTCTTTTTAGATGCAATAAATAGAAAGCATTTGCATTTTGATATTTATTTAGAAAACAAGAAAAATTTAAATCAATCATATGTGGTAAGAAATTTTACCATTGAAACAACGAATGTTTTAAATAATGAAATGTTTTCTGAAACACAAATTGCCAAGTGAAACGAACAAGATCCCAATATTTCTAAGGCAGCTTTAACTAAAAATTGAAATTTCTATTATGTTACTAAAACAAATGCAAACCTAAATTTTGAAAATGATAAAACATATGAAGAACTTAGAAACAAAATAAGTAATTTTAATAATAAAATTTTGAATAATAGTGATTATAATTACGTTGATAAATCGCTTTGATTAACATCTCATCCTAAAAAAACCGATGAAGCCGAATTAAATTATTTTAAGTTGTTTCAGTCAGATGTAAGAGACTGATTTGTTCCATTTAATTTCTTAAGTCAAGGAATTCAGTTGAAAAAGATTGAAAAAAATGAAAATAATATTTTTATTAAGACGCTCAATTTAGAATTCAAGTCAACTTTTTATACTATTGTAAAGAATAAATTAATTTTAAAAAATAAATCTAGTTATGAATATGCTATTTATGTTCCATTTTTTACAAATAAGGATAAGACCATTAAAGACGGCAAGGAAATTTATCAACCTAGAGTTTTTGAATATAGTTGTCCTATAACATTTGTTAATAGTAATTACAAAAAATTTAAATAAAAAAACCGTTTAAGTCGGTTTTTTATTTAACTAAATTAAATGAGAATTTTTTTCTTGCTTTCAAGGTTGATTTTTATTGATTCTGTCTATAAATAATTTTCCTTGCAAGTGGTCATATTCGTGTTGCAATACAATTGCCGGATAGCCGGAAACATCATAAATTTTTTCTTTTTTTTCAAAATACGAATATGCTTTTATTACAACTCTTGAATATCTATATACAAAACCTTCTTGACCTGCATCGCTTTCCTTAACGCTTAAACATCCCTCCCCTTCTTGCAATGATATTTTATGGTTTGAATGTGAAAGCATTATTGGATTTATTAAAGCGTCTCTAAAAATATAATTATTTTGATCGTCTTTTACCATTACATAAAAAATATTTTTCAATATTCCATATTGAACTGCAGCAACTCCTACGGCAGGTCTAAATTTTGTTTGAGGAGTTTGACTATCATCAACATGAAAAATCATTTTTTGTATTAATAAATCATCTTCTGCGGTCAATGGGAGTTCAACGTTCTTTGATTTATTTCTCAATACTTTTTCTGGTAATTGAACTAATTTAACATCTTCAAATTTAAACATAGTTAAATTATTACATATTTAATTGAATTTAAATGAACTTTTTAGAAATATTTTATTATAATTAGAGATTATTTTAAATATAGGAGAAATTAATGCCAGAGCCTTTATCCGGTAACTATAATACTTTAAATTCAGCATCAGGAGCTTCGACTTCAACCGGTGCACCATGACAATACATTTTATATTCAATCATTCTTTTTTTACTAATATTATTTAGTGCTATATTTTCAGCTTCAGAAACAGCATATACAACCGTCTCAAGGGCTAAGATTGAAAATATGATTGAAAAAAAGCAAAAGTTCGCTAAATTAATTCAAAAACAACATTTATTTTTTAATAGAATTTTAGGGACTATTTTAATAGCTAATAATTTGGTAAATATTGCATCTGCAACATTGTTTTCATATGTTTTATCATTAACAACAATGTCTTCTGGCAAGGCTACTATTATTTCAACTGCTGTTATGACTCCACTTATTGTTTTGTTTGCAGAAATTTTACCAAAATTAATTGCAAAAGCATATCCCGAAAGAGTTATTAGATCAACTTGTTGATTTATTGAAGCTTTATATTGAATATTTTTTCCAATTACTTATCCAATATCTAAAATTGGCAAAAAGATTTATATAACAAATACAGAAGAAGATATGAAAAACTTGATTAATATTGCACAAGATGAAGGTGTTTTAGAAATCAATGAATCAACTATGGCAAAGAATGTTTTGGATCTTGATTCAAGTAAGGTTTCTCAAAATTACGTTAAGTTAAAAGATATTGATTATTTGAATTATAAAGCAACTTTGCAAGATGCATTAAACAAGTTTAAAGAAACTAATTATTCAAGATTGCCAATTGAAAAAGATGGAGATATGATAGGCATATTGCTTTTAAAAGATATATTCTTTTTAAAGCGTGGCAATATTATGAATTATGTTAAAACGGTGCCAAAAATTTCTGCAAATAGTTTACTTTCTATTGCATTAGAAAAATTAAGGAAGGCCAGGGCACAAATGGGTTTCGTAATTAAAAATAATAATGATGACAGTGTCATAGGAATAATTACAATCGAAGATATCATTGAAGAAGTTGTAGGCGAAATATACGACGAATATGATGATGATGAACAAATTTATGAAATAAGTTTGGAACGCTGTGAGGCCCGCGGAAACGTTAAAATTTCAACAATTTGAAAGCGCTTTGAGTGAGATGAAATTTTAAATTTAGAAGCTCCTAATGATGAGGATTTAAATTTATCAAATTGACTAAAAAACCGTGTTGGCCACGAGCTACATGTTAATACAAAATATACTTACGAAGATAAAATTGATTTTCAAGTGATATCATTAAAAAATAAAGATCAAAAATTTGATATTATCGATATAGATTGAAGCAAATAAAACCTGTTTTTCTTAACGGGTTTTTTATATCTTTATTATTTATTTTATAAATAAACATTCATTTTATACAAAAAAACTATTTTGTGTATATAATGTAGAAGTTAAAAATATTTTTAACAAGGTTTATGACTCCTTGACCGTTAGGTCCAAATGTCCAAAAGGAGAAAAATTATAATGTCAAATTACGAAATTATGATTCTAACTAATCCAAAAACAACAGATGAAGAATTACAAAATCTAGTATTTTCAGTATTAGATAAAAATTCATCAAAATTTGAAAGATTAGAAAGAAACGAAACTGCATATCCAATTAAAAAGTTAACTCATGTAAATTACTTTTTAATTACAACAAAGGCAGAACCTCATCTAATGTCTGAATTAACAAGAAAGCTTAATATTGATAAATCAGTTTTAAGATCTTTAATTATTAATTTAGATTCTGAAAAAGGTTTAAAGCCAAGAAAAGTTTCTAAGAAATTCAATAAGAGAATTCCTCTAAACCGTGAAAATAAAAAACCTTATGTAAAAAACAACGAAACTTCTAAAGATGCTAGCGAAGAAAAGAAGGTTGCAAGAAAACCAAGAACTTCAAAAGTTTTAGACGAAAAGAAATAGTTAAAAAATAATAAGATCGGTAGGAAAAATGAATAAAGTATTTTTAATTGGTCGTTTGTCGGCCAAACCTTATAAAAATGTTACACCTTCTAATGTTGAATATTCAAGATTTACTATGGCTATTAGACGCGAATACGCCGCCCCTAATTCTGAACCTGTAGTAGACTTTGTCCCATGTGTTGCTTGAAGATCTAACGCAACTTTTATTAATAAATATTTAGATAAAGGTTCGCTTCTTCTTGTTGAGGGTACATTTCAATCTTCACGTTTCAATAACGGCGATGGTCAAATTTCAACATCATATGTTGTTAGTGTAGAAAAAATTCAATCACTAGAAACACGTGAAATGTCAGAAAATAGACGAAAAAATAACAATGGTAAAGAATTTAATATTGCCACAGAAGAATCAAACACTATCGATAATAATTTAGAAAATAATCATCAATCAAATGAATCAAATGATAATCCTTTTGATCAACTTGATTGAGATTTATAGGAGTTAAAATGGCAAGAATTGTTCGTAAGAAACCTTTTATTCGCAAACGTCCATGTCAATTTTGTTTAAGTAAAGAACCAGTTGTTTACATTGACTACAAAAATGAAGAAGTTTTATCAAAATTAGTAAATTTACAAGGAAAAATTTTATCATCACGTATTACCGGAACATGTGCAAAACACCAAAGAGCGGTTGCATTAGCAATTAAAAGAGCAAGATTTGTTGGTATTTTGCCATACATCGGTTTAGTAAAAAGAGAATCTAAAAAAGAAACTGTTAAAACTGAAAAAGTAGAAAAAGCAGAATAAAATAATAAAAAAATCAAGTGTATGTATTTGTTATGTAACTTGATTTTTTTATTATAATTTTTAGTAATCATGAATATAAAAACCAAGCAAAAACTAGCAATATTTATTGGCGTTGTCAATTTTTTAACAATCGTAATTTTTTTATGTTGATGTTTAACTTATTTTTTAGAAAAGAAAATAGATTTTAAATTATTTGGTTATATTTGTTTAGGCTTTACATTGGTTGCTTTTTGTGTAAATATAGTTGGATTAATTTACACTACAAAATATGATATTAAAAAACCTTGAAGAGTTTTTTTTATAAGCCTTTTAATAATACCTTTAGGATTTTTTGGGGCAATAAGCTTAGCGTTGAATTATCCAGAAATTAAAAAAAATAGGAGTAAGAGTTAAATTAATATGAATAAAAAAATAAGTTTTAGTCAGATGATCTTAAATTTGGTGGCATTTGTTTGCTTCATCGTACTTATTTGATCTATTGTGGCCAATATTTCCTTTAAATTTACATATGAAAAAAATTTTCAAAATCCAATTTCGGTTTATGAAACTAATAAATTTAATATGATCAATAGAGTCTTAACGACTTTTTTAGTATTGTATATTTTATTTACTATTGCTTCTATTTCACTGAATATTTATGTTTCAATAATTTTATTCAAAAACAAGTATGCAAAACTCTTTTTACTTTCATTAGCAAGTGTTTTAGTTTCAAGAATAATTGGTATTTTTTGTGGAATCATTTTGCTTTTAAATAAAAGACTTTCAACAAGCAAATGCTAAAAGTCTTTTATTTTTTAATAAAATAAAAAAATTAAATATTTTATAATTTAACCATATGGATTCTAACTTAGCAAATTTAATAAGGCCAAAAATTTTAGAAGATTTTATTTGCCCACAAGAACAAAAAAAATTATTTCAAAAAATTATAGAAAATAATGATTATCGTTCATTTATTTTTTACGGAAAGCCAGGTACTGGAAAAACAACTATTTCCTATATTCTTGCCAATGCCTTGAAAGTAAATTACGAATATTTTAATGCGGCCATTGAAAATAAAGAAGATCTTGTACATAAATTAAAGTTGAACAAGATTTTGATCATTGATGAGATTCATAGATTAAACAAAGATAAACAAGATATATTGTTACCATATCTAGAAAACGATTTAATCACAATTTATGCAACTACAACGGAAAATCCATATTTTAAGCTTAATCCTGCATTGCGTTCAAGATGTTCAATAATTGAAATTACTAAGCCAACAATTCAAGATTTATCAAGTTGTTTAAAAAATGCCTGCCTTAAAACTTTTGGTGTGGATCTTAATAAGGATATTGCTGATTATTTAGCCTTGCAGGCTAATGGCGACTTTAGATCTGCATTAAACAATCTTGATTTGATTATGTTAATTTTTAAAGATCAAGAATTTTCGTTGGAGGCAATTAAGAAGATTATTCCTTCAATTCAATTTAGTTCAGATGAATCTGGCGATAATCATTATGATTATTTGAGCGCTTTTCACAAATCACTACGCGGTTCTGACATTGATGCAGCATTATATTGAGGATTTATCATTATTAAATCCGGAGATTTTGATGGTTTGTTTAGAAGAATGTTGTGTGCAAGTTACGAAGATGTTGGAATGGCAAATCCTAAATTGGGATCAGAGGTTTTGTCAGCAATTGAATGTTTTGAAAGAATAGGAATGCCTGAGGGGTATTTACCAATAACACAAGCAATATTAAATATTTGTTTAAGTCCAAAATCAAACACTGTTTATAGCTCATCAACATTAATAAAATCTATAATTGATAGTGGAAATATTTTTGAAGTGCCAATTCATTTAAAAGATGCTCATTATAAATCGGCAGCAAAACTAAAAAGAGGATTAAATTATAAATATCCTCATGATTATAAAAATAATTATATTGATCAACAATATCTACCAAATGAATTAAAAGATAAAATTCATATTGAATTAAATGGGCAAGGTTATGAACAAAAAATTAAACAATATTGAGATTTAATAAAAAACAAAGGAGAAAAATAAAATGGAATTTGAATTAAATAAAATAAATACATTGGAAGATTTAAAGTTAGCTAAAAATGCTTTTAACAATTCAGATGAGTTAAAAGAATTAATGTCTCAAATTAAAACAGCAGATCCATTGCAAAAAGCAAAACTTGGTAAAAAAATTCAACTGCTAAAAAAGCAAGCAGAAGATTTTTTTGATTTAGCAAAGCAAAAACTTGAAGATATAAAAATTCAAGATTTAATTAATCAAGAGTATGTAGATTTTGCAAATCCTATAAATACAACTGGAGGAATTCATCCAATTAATTTGGTTGCAAATAGATTTAGAGAATGATTATTGGCAAATGGATATTTTGAATATACAGGGCCAGAAATAGAAAATGAAGAATATAACTTTGAACGTTTGAATATTCCTCAATCTCACCCTGCAAGAGAGATGCAAGATTCACTATATATATCAGAAAAAGAATTGCTTAGAACTCACAATACAGGATTTAGTGCAAGAGCATTAGAGGAACATAAAAATCAAGAGTTCTCACAATTTGCTATTGGAAAAGTTTATAGAAATGATGAAGAAGATAGAACACATACTCACCAATTTACTCAATTAGATTTTGTTAGTGTTGGCAACCATTCATTTGGAACATTGATTTATACATTGACTGAATTATTTTCTTATGTTTTAGAACAAAAGGTAAAAATTAGACTACGTCCTTCCTATTTCCCATTTACTGAACCTAGTGTTGAGGTTGATGTTTTGTTTAATGAAAAATGAGTTGAAGTATTGGGCGCTGGTATGTTGCATGAAAATGTTTTGAAAATGGCTGGATATACCAATGATATGAATGGCATTGCTGCAGGAATTGGTATTGAAAGAATAACAATGATAAAGTATGGCATTGACGATATAAGAGAATTGTATGGAAATGACCAAAGATTTTTAAAACAATTTAAATAAAAGGAGCAAAAATGTTATTTTCTTATAAAAAAATAGTTAAATTAGCAAATATAACTGACAAAAGTGTAGAAGAGGTTGTTGGGGCAATTAATTCAATAGGCTTTGAAGTTGAAGAATATCATAAGTTTCTAGATGCTGAAGGTATGAAATTCTGTCATGTATTAAAAGCATACAAAAATCCTAATTCTGATCGTTTAACGGTTTGTGAAGTTGAATTTGGCAATGGAAATCATTCAATAATTCAAACTAATGCAACAAACATGCATGAGGGCGATTATGTAATGGCTTATGTTCCTGGATCGAGATCTGGAAAAAATATATTTGCTGCCAAGGAATTAAAAGGAATCATCTCTGAAGGAATGTTTGTAAGTTTGAATGAAATTGGTATTCCAGAAGAATTTCATCCCTTAGATTCAAAAGATGGTATTTTTCAAGTTGGAAAAATAGATTTAAATTTAGACCCTGTTGAATATTTTGATCTTGATGATTATTTGATTGATGTAACAATTTTATCCAATAGAGCTGATGCTTTGTGCTATTTAATTTTAGCTAAAGAAATTTCAGCATATTTTGAATCTTATATTGAACCTCTTCCAAAACCAAAACCTACCTTGGAAAGCAATATTGTTGTAAGCAAAAATTTAGTTGCTACAAACTGTTTTAGTTTAGTTGAAGCTATTACTAAACAACCTGCTATTTCTCTTCAAGATGAATTTCTTTTATGAAAACACGGAATAAAAACATTTAGAAATGCAGTTGATATAACCAATTTGGTCTTATTATACGCAGGAGTTCCTTGTCACGTATATAATAAAAATTCTTTAAGTTCAAATGAGTTTTCTACATCATTGAGTAATGAAACATTAAATGTTTTAGGCAATAAACAGGTTACATTAAACAACAATTTGGTTGTAAAAAACGGAGATCAAACCGTTTCAATAGCGAGTGTTATAGGCTTAGAAAATTATGAATATAGACCTGATGGTGGACATTGCGTATTTGAATTAGCATCATTTTCATTAAAAGAAGTTAGAAAAAGTATAAAAACAGTCAAATTAGAAACATTAGCATCTTTACGTGGTTCAAAAGAAATATCAAGCGGTCAAGTAGTTATGGCATATGAATTTTTAACTAAATATTTAGATCAATATTCAGTTCAAATAAACGCGCCTAAGCTAAGAAAAACAAATATATTAATTGATAGCCATTATATTACTAAATATGCAGGCTTCAATATTACGAAAACAAAAAAATATATTCAAGTTTTGAATCAATTGACTCTTTTGGGATTTAAAATAAAGGAAGATTTATCATCAGTAATTTTCCCAACTTATAGATACGATTTAAAAAATATGCAAGATTTTGTTGAAGAAGTATTTAGATTTTATGGATATGATAATTTCCCAGCTAAATCTCCAAAAATTACTAGATTATTAATTAAAGAAAATAAGGAATTTAAGTTTATTGAAAACTTCTGTGCAAAAGGATATTTCAATGTTAGAACATACACGTTAATTAATCCTGAAAAAAACATTTTCAATCCATTTGATTTTAAAACTAATTTCAATGCAATTGCTGCAAAGAATTATGATCATTCTCAAATTAGAAATTCAATGATTTCTTCATTATTAGAAGTCTTAGATACAAATATCAAACAAGGCTTGCCAGACGCTTCATATTTTGAAATAGCTATGATTAATAATAAAATGAATGTATTAGGAATTGCTTCATTAAATAAAACTATTGATGATATAAAAGCTGATATTTGTTCAATAACTAATGAAACATTAGAATTTAAACCAAGTAAATTAAAAATTTTTAATCCAAATGCAGCACAAGAAATTTATTTAAATAGCGAATTAATTGGATATGTTGCAAAATTAGACCCTCACTATTGTCAATATAATGCAGTGTTTAGCGAAGTCTTTTTGGATAAAATGAATAGTAATGTAATTAAATATAAAAATTATAAACATACACCTCTTAAGTCTAGAGATGTTACTATATCTGTTTTAGATAAACAATCTATTGAAGAAACAATTAAAAAAATACAAAGTATTTTTGGCGTTTACAAAATTTCAATTAAGGATGTTTATAATAAAGATAATGGCACAAAAAATATTACATTAGAAGTAATTTTAGAAGATTGAGCAACCAAAAAATTTGATTCAATTTTTAATAAATAAAATTAAAAAATGGTAAAAAAAGCCATTTTTTCTTTATTTTTTCTCTATTTTAAATTTTTAGGATATTTTATGAATATACTTAATTGCAGGAGTGAGAATGAAAGAAACAACAAATGATAATCTTCAAAAAGAAAAGAGCATTATTGCTGAAACATTAAAGGAAATAGAAAAGAAATTCGGCAAAGAAGCCGTTATGATTTTAGGAGAAAAGCCAAGCATAATTCCTGAAACTTTTAGCAGTGGTTCGTTGACTATCGATAGAGCGTTAGGAATAAATGGATGACCAAAAGGCAGAATTATTGAAATATATGGTCCAGAAAGTAGTGGAAAAACAACATTAGCGTTGCACGCAATTGCTGAAATACAAAAAACTGGCGGAATTGCAGCATTTATTGATGCTGAGCATTCTATAGATCCAATATATGCCAAAAATTTAGGGGTTGATATTGACAATTTAATTTTATCTCAACCAGATTCAGGAGAACAAGCATTAGAAATTGTTGATACATTGTCTAAATCCGGAGCAATAGGACTAATCGTTGTTGATTCGGTTGCTGCATTGGTTCCTGAGGCTGAGTTGAATGGAGAAATGAAAGACCAAGTAGTAGGTGCACAGGCAAGGCTTATGTCTAAAGCTTTAAGAAAAATAACTGGATCATTATCAAAAAACAAGACAACAGTAATATTTATTAATCAAATAAGAGAAAAAATTGGAGTTATTTTTGGCAATCCTGAAACAACAACTGGCGGAAGAGCATTGAAATTTTATTCAAGCATCCGTCTTGAAGTTAGAAAAATTCAAAATGTAACCAATAATGGAGACATCACAGGTAATCAAATTAAGTGTAAAGTTGTAAAGAACAAATTAGCTGCTCCATATAAAACTGCTCAAATTGATATTATTTTTTCAAAAGGTATTTCAAAAGAGCTTGAAATAATTAATTTAGCAGAAGAATTAGGAATTTTAGTAAAAAAAGGCTCGTGATATAGTTTTAATGGCGAAAATATAGCACAAGGGGTATTAAATTTACAATTTATTTTGCAAACTAATAAAGAATTATTCGATAAATTAAAACAAGAAGTTTTAGACAAATTAAATTCGATAAATTAAAATAGAAATTTAAACATATAAATGTTTATAATTTAAACTATGAAAAATGAAAATATAAAAATTCTATTTTTGGGAGACATATTTGGTTTACCGGGAATTAGATTTGTTGAGCAACAATTGCCAAACATAATAAAACAAAATAATATTGATTTTGTAATCGCTCAAGCAGAAAACGTTTCGGGAAGAAAGGGTTTTGAGCCTAAAGATTACGAAAGATTGAAAAGAGCGGGAATAAATGCATTTACATTAGGAAATCACGTTTGAGCCAAAGAAAAGATTCATCAAATAATAAACAATGGAGATTTAATAAGACCAGCAAATATAAATAGTTCATATCCTGGTAAAGGCATAAGAATATTTGATATTAAAGGTCATAAAGTCGCAATAATGTCTTTTATGGGCATAACGTTTAATCCTCTATTAGAGCCATGAAAAGAACAAAGCGCAAATAATTTCTTTGATGAATTTGACGAGTTATATGAAAATAACGGCGCAGATTATTACATAATAGATTTCCATGCTGAGACAACATCTGAAAAAGCGGTATTTTCACTATACGTTGATGGTAAAGTTGATGCAATTTTTGGAACGCATACGCACGTTCAAACAAACGATGCAAGAATTTTGCCTAAGGGTACATATTTTGTAACTGATGCCGGAATGTGTGGCCCAAGAGATTGCGCTATTGGTTCTGACTTCCAAGAAGTATATGAAAAAATGCGTTACGCAGCATTTTCTGCATTTAAGGTATCAAAAAATAAATGTCAATTAAATGGAGTTATTTTTACATTAACCAAGGATCCAGCAGAAAAGCATATTGAGTTAGTAAATATAAAGGAGCAATAATATGAATAACGATTCATCATCAAAAATCAGTGAGAAGGATAAACGAATTTTATTTCTACTCAATAAATATAAGGCAAGGATGCATATTATTAATTGCATATATCAGAGCGAGCTATTTAATAAGGATCTTTCAATACAAGAAATTTTGGAAAATTGTGGAGAAAATCAATTGAATGCGAATGAAATAGAATCTTTAAAAATAATTGAAGCTAAATATGATGTTTTTACTAGAATAGCAAAAAAATTTATTAGTGAAGACTGAGAATGAAATAGAATTTTACCACTATCGAGAGCCATAATAATATACGGTGAATATGAATTATTATTTAATGACCCAAGGGTGGTAATTAATGAGATGGTTAATATTTCAAAAATATACAGTCCTAATAATGATTATAAATTTATCAATAAGGTTCTAGATCTTGTTTCTAAAAATGTAATTAAAAAAAATACTAAAGATGAAAAAGAAAATTAGCGAGATAATAACCAATATTTTTAAATACGATTTGAAAATTTCAGAAGCGTTAATTAGAGAAGGAAGAGTAATTGTAAATAATGAAAAGATAATTATTCCATCATTAAAATTTGAAGAAACCGATGTAGATATAAGATTCATTAATATGAAATTGCCCTATGTTTCAAGAGGCGCTTATAAATTATTAGAAGCAATAGAAAAATTTAATATAAACTTAACGGATAAAATATGTCTTGACATAGGATCGTCTACTGGGGGATTTGTTGATGTAATGTTAAGGCATGGTGCTAAAAAAGTATATGCCGTAGATTGTGGCACAAATCAACTAGCATATGAACTTAGGATAAATGATAAGGTTGTTGTTTATGAAAAAACTAATCTTAAAAATTTAAAAAAGGAAATGTTCCTTGAAAATATTGAATTTGTATCCTGCGATGTTTCATTTATAAGTTTGAAATATGTATTTGAGGTTTGCAATAAAATATTAAATAATAATATAAATGTAGTTTTTTTAATTAAACCCCAATTTGAAGCTTCAAGCAAATATGTTGAACCCGGAGGATATGTTAATCAAGAAAATCATAAATATATTATTGATAAAGTAATTTCATATGCAAAAAATAATGGCTTTGTTTTGATTGATTTAATAAAAAGCCCTATTTTAGGAGAAAAATCAAAAAATATTGAATATTTAGGCTTTTTTAGAAAGGATAGTTAATGAATTATAAGGATTTATTTCCTATGTTTAAAAATAACCCTGATATTGTATATTTCGATAATTCAGCACTTACTTTTAAACCACAAACCGTAATAAATTCAGGTATTGATTTTTATGAAAAATATTCAATTTCTACAAGGACTGCTGATAGTGTTTTAGGAATCCAAACAGAAGAAGTAATGCATGAAACTAGAAAAAAAATTGCAGATTTGATTGACGCTTCTGCCGATCAAACTATATTTACTTCAGGAACGACTGATGGATTAAACTTAATATCTTTAATGTTAAAAGATATAATAAAGGATGGCGAAATAATATTTAGCTATTTTAATCACAGCTCAGCAATCGTTCCATTCATTGAAAATCTTAAAAACAATAATATAACATTTAAATATGCAAAAGACAACGAGGAAGTTCTAGATTTAATAAATGAAAAAACAAGGTTAATTGTATTGCCTCAATTAACTAATAATTTTAATGTATTTTTTGATTTGAAAAAAATTCATCAAAAATCTAAAAAATATGGTGCTTATTTGATAAATGATGCCGCACAGGCTATTGCTCACGAAAAAGTTTCATTGAACGACTGCGATGTAATTGTTTTTTCAAGCAATAAAATTTATGGTCCTACCGGTATGGGAGCTTTAGTAATTGAAAAAGATTTACTAGAAAAAATAGTTCCTCAAAAATGAGGTGGTGGTCAAGTTCAATCTATTGGTTCTGGTTGCGAATGAAGTGCCAAGAATGATATAAAAAAATTTGAACCTGGAACTCCTAATTTTGCCGGGATTTTTCAATTTAACGCTGCAATTGATTTTATAAATAGTCTAGGTTATGAATTAATAAATAAATTAGAAACAGCTGTTGCAAATTATTGCTATGATGAATTAAAAACAATAGATGGAATAGAAATAGATTCAAAAAGAGGGGATAAAATTATTTTATTTAATATAAAAAATATTCCTTCTCAGGATATTGCATCATATTTAGGACATAATAATATTTATGTAAGATCTGGGGTTTTTTGTGCACATAAATTTCATGAATTAAAACAATATAGCAATAGTTATGTAAGAATTTCAATTTCATTTTATAATGATTATAATGATGTTGATAAATTCATTAAACTTTTAAAAGAAATGGGGGCTAATTTTCTTGAATTTTTATAGTAATATTGAAAAACAAAGAATAATTTTTGAAGCATACGAAAACCCAAAATTTAAAATAAAAAATGATGAAGATTTTAAGAAAACTGATAAGACATTATTTGAACATTCAAATGTATGCGTTGATGATATTAAGCTAAATTTTTTATTTGATGAAAAAAATACTTTGAAAAAAGTGGAATATTGTGCTAAAGGGTGTTCAATTTTTTTGTCATCCGTTGAATTAATGATTCAGCAAATTTTGAATAAAAATAAATTAGAAATAAATGAAATTTTAAATGAATATAAAACAATGATAGATACATCTACAATTTCACAACGTGGTGGAGAAATATTGGGAAAATTGGTTGTATATCAAAATGTAAGAGTTCATTTAAATAGGTTAGAATGTGCATCAATAATTTTTAGGTTAATAAAAAAAGCAGTTTCTAATGAATAAGATAATTTTTCACATAGATATGGACTCCTTCTTTGTTAGTTGTGAACGTTCTATTAATACAAACCTGATAGACAAGCCTGTTGTAATTGCTAAAAGATACAAACGTTCAATAATTAGTGCAATGTCTTACGAGGTAAAAAACCTTGGATATAGAGTAGGAAGTTCTTTATACACCGTTTTAGAAAAGGTTCCTAATTTACTTATAGTAGAACCACACTATGATTTATATTCTCTAATTTCTAAAAAAATTTTTGATTATTTAAAAAATAAGTATTGTACACAACTAGAAATATATTCAATAGACGAATGCTATATTGATTTTAGCAACACTATTAGCAATTGGGAGGAAGCTAAAAATCTTGCCCAACAAATTAAAGATGATATTTTAAAAACATTTATGATTCCTTGTTCAATAGGAATTTCCTTTACAAAGTTTTTGGCAAAAATGAGTACAAATAAAGCAAAACCATCAGGAATTTTAATAACAAAAAAGGAAGATATTGAAAGAAATTTTTACTCATTGCCGGTGGAAAAAATATTTGGGGTAGGAAAAAATATTTCGCAACAATTAATAAAAAATAAAATTTCTACTTATAAAGATTTGCTAGAATGCAATAATGAAATTTTTTTAAGAAAATTGTTTAAAAAAAATTTTTTCTTATTTATTGAACAACTAAAGGGAATTAATGCAACCGACCACATCTTAAATCTTGAAACAAAAGGAATAAGTAACACGATAACTTTTATGGAAAAAGATTCTGATGATATCGTTTTTTTGAAAAATGAATTGTTTAAATTGGCAAGAAATGTTGCTAATAGGGCCGTAAACAACAATTATGAATGCAGATGTGTTGAAGTTTTAATAAGAAAACAAGACAAAAAATGAATGTCAGAGCAAAGAAAAATTGATTTTTTTACAAATGATATTGACATTATTTTTAAAATTGCGTTGAAACTTTTTTTAAATTTATGAAATGAAAAAGAATGTTTAAGGGGTCTTGGAATTAGAATCTGCGATTTGAGGTCGATATTTCAAGAAAACAAACAAGTTAATTTGTTTGAAGACAATAATCAAAAATCAATAGTAAATTCAATATTAAATGAGGTTAATACAAAATTTGAGGGTAAAATTTTAATAACAGGGAAACAATATTTGTTAGAGAATTCAATTGATATTGATAATATAAAGTTTTTAAGAAAAAATAATCAACCCTATGATAAAAAAATAAATTTAGAGGAGAAAAAATAATGAAGATAGGAATATTTGGCGGAAGTTTTGATCCTGTTCATAAAGGACATATTTTAATTGCTAATGATGCAATTGAATTATTAAAACTGGATAAAGTTATTTTTGTTCCTGCTAACAAAAATCCTTTTAAAGATAAGCAAGATTATGCATCTAATGAACATAGAATAAATATGATTAATATTGTTATAAACAAGAGTAATATGGAAGTTTCACAATTTGAAACAAAAAGAGGTGGCACTTCATACACAATTGATACTGTAAAATATTTTGCTCAAAAGTATCCAAACGATGAGCTATATTTTTTAATTGGATCGGATAATGTTGGCAGTTTAAACAAATGAAAAGATATAGAAGAAATTTCAAGAATTGTAAAAATTGTTGTATTTAATAGAAATAATATTTATTCAAAAATCAATATTAAAAAATACAATTGTTTAGTTTTAAAAAATTCTTTTCATCCTTTTTCTTCAACTTCTTATAAAAATGGAAATATAAACGTAGTAGATGAAAGGGTTCAAGAATACATAGGAAAGAATTGTTTATATTTAGAAAATATTGCCAAAAATACTCTTTCTATTCCTAGATTTAAACATTTAAGATATACTGCTGAATTTTCTGCACAATTGGCTAAAAGAAATAATTTTGATGTCGAATTAGCGTATAGAACTGGTTTTATGCATGATATTACTAAAGAGTGAGACATAAATAAAGCATATAAATTTTTAAGTTCATATGGGTATAATGAACAAAACTTGCCAGAATATATGCTACACCAAACTACGGCCTATTATTGGTTGAGGGATGTATATAAATATCAAAATGATGAAGCGTTACAAGCAATCAAAATTCATACATCATTAGATTTTAAAATATCTTTATTGGCAAAAATTTTATATATTGCTGACAAAATTTGTGAAGGAAGAAAATGACCGGGAATTCAAAAAATTAGAGAGCTTGCCTTAAATAATTTAGAAGAAGGTTTTAAGGAAGTAATTAGAATATGCGGTCTTAAATTTAATGAACAAAAAGGCATTATTTTTGATGAAAAGCAAAAAGAAATATATAAGTTATTATTAAAATAAAATGTGTTATAATGTATATGCTTTAACTAGCATAAAGTAGATTCATTTCTCACCTTAGTGATTTTTTGCTGGGTTGCTACAATAAAGAATTTGTAGTTTTTGGGAAGTGGATTAATACCACTTTTTTATTTATAAAAGAAGGAGGAGTTATTATTAATAACCAAAAAATTGGAGACGATTTAAATAACAATCAAACAAATCGTAAACCAAAGTCAGAACATGTTATCAACAATGCTATTCCATACAAAAAAGTTTTTGTTCTAGGACCTACAGGGGAAAAAATTGGTGTGTTAACCAAAGAAGAAGCTTTAGAAAAAGCATCTGAATATAATATGGATTTAGTTTTAATTGCAATAGAAAACAATAAGCCAATAGTTAGAATATTAGATTATGGCAAATTTAAATATGATAAGAAAAAACGCCAAAAAGAAGTTAAAGAAAAACAAGCAGTTACTGTTAATAGAGAAATTCGCTTAACTCCATTGATTGGTGATCATGATTTGAAAACGAAGGCAAACAAAACTAGAGAATTTATTTTAGATGGTAATAGAGTTAAAGTTTCAGTTAAGTTCCGCGGTAGAGAAAGAGCAAGAATTGAATTAGGGGAAGAAATATTAAAAAAGTTCTTTGCCTTAATTGAAGATGTTGCAAAAATTTCAAAAGAAGCAACATTAGTTAATGATAGATTTTTAGATATGTATGTAGAAAAAGATAAACGAAAAGTTGATACATTAACCAATAAAAAAGATAACTAAACCTTCAATAGCATAAGGAGAATAAAATGCCAAAAATGAAAACCAAAAGCGGTTTGAAAAAAAGAATTTCAATCACTGCATCAGGAAAAGTAAAACGTGGACAAGCGTTTAGATCACATTTAGCACAAAACAAAACAACAAAACAAAAACGTCACGCTCGTAAAGCAACAACTTTATCAAGTTCAGATTTTAAAAGATATAAAGAATTAATTTAGAAAGGATAAATTATGCGTACTAGAGGCGGAATTGTAACAAGAAGAAGACGTGCAAAATGAATAAAATTAGCTAAAGGATATTGAGGACACAAATCAATAGGATATAAAGTAGCTAAACAAGCAGTTGTTAAATCATGGACATATGCATTTAGAGACAGAAAACAAGTTAAACGTGAATTCAGAAAATTATGAATTGCTCGTATTAACGCAGCTGCAAGACCACTAGGAATTACCTATTCAAGATTAATTGAAGGTCTTAAAGAAGCTAACGTGTTAGTAAATAGAAAAATGTTATCTGAATTAGCAATTAATTATCCTACATCATTTGCTAAATTAGTAGATCAAGCAAAATCAGCTTTAGCAAAAAAATAAATCTGTAATGGATTTATTTTTTATTATATTTATTAATAATTTATTAATAAATTAATTAAGTTCTTTATAATTTTATTTATATTGCGTAATGGAGGAAAATGAAAAAAAGAGTGGTATTAGGAATGAGTGGCGGAGTTGATTCTAGTGTTTGTGGATATTTGCTACAGAAACAAGGATATGACGTTGTTGGTTTGTTCATGAGAAATTGAGATTCATTTTTAAACAATGATATTTTAGGAAATGAAAATATAACTCAAAATATTTGCCCACAAGAGCAAGATTATTTAGATGCAAAAGCGGTTGCAAGCCAATTAAATATTCAATTATATAGAATTGATTTTGTTAAGGAATATTGAGAAAATGTATTCAATTACCTAATTGATGAATATAAAAAAGGTAGAACTCCCAACCCTGATATTTTTTGTAATAAATTTATAAAATTTAAAGCTTTTTCTGATTATGCCTTTAAAGAATTAAAAGCCGATTATATTGCTATGGGGCATTATGCAAATGCAATTGACGGAGAATTATTTCGGGCGCAAGATGATAATAAAGACCAGTCTTACTTTTTAGCGCAATTAAGCCCTGACCAACTTAAAAAAGTAATTTTTCCATTGGCTAATATTACCAAACCAGAAGTACGAGAAATTGCAAAACAATTAAACTTGTCAACCGCTATGAAAAAAGATTCAACAGGAATTTGCTTTATAGGTGAAAGACATTTCACGAAATTTTTAGAAAATTATATTCCTGCTCAACCGGGTAATATCATTGATATATTTAGCGGGAAAGTGGTTGGAAAACATATTGGTGCGATGTATTACACTATAGGTCAAAGAAAAGGTTTAAATCTTGGCGGGTATGAAAAACCATATTTTGTAGTAGGACATAATCTTTCAAAAAAAGAAATTTATGTAACAAACGATGACCATATTGACTATTTAATGTCAAATAAATTAATCGCAACAAATTTTAATCAAATTTCAAAAGAATTTAATTTTGCTAATTTAACTGCTAAGTTTCGTTATAGACAAAAGGATATCAAAATAACTTTAAAAATTTTAAATGATAATAAAATAGAAGTTTGCTATCCAAATACCGAAGCAGTAACTCCTGGCCAACAAGTTGTAATATATGATGGAAATAAAGTAGTTGGTGGAGCGATAATTGACAAAATTTATTTAAATAATGAATTAAAAACGTTTTTATAAAGGAAAGGAAAATAGAATGCTAACTTCAAAAGAAATTAGAAAAATGTGATTAGATTTCTTTAAATCTAAAGGACATTTAGTGGTACCTTCAAAATCTTTGATACCACAAAATGACCCCTCTTTGTTATGAATTAACTCAGGAGTTGCAACATTAAAAGATTATTTTAGTGGTAAAAAAATACCTCCTGCTAAAAGAATTACCAATTCTCAAAAATCAATTAGAACAAATGATATTGAAAATGTAGGAATAACAGCAAGACACCATACATTTTTTGAAATGCTTGGTAACTTTTCAATAGGCGATTATTTTAAAAAAGAAGCAATTGCTTTTGGATATGAATTTATTTTTAATGTTCTAAAACTAAATAAACAAAATATTTATATAACTTATTATTCAGAAGATAAAGACACTTATAATTATTGAATTTCAAATGGTATTGATCCTTCGCATATGATAAAAGGCAGTAGAGATACAAACTTCTGAGATGTTGGCCAAGGACCATGTGGCCCTGATACCGAAATTTTTTACGACCGTGGGACAAAGTATGATACCCGTGGCATTGAATTGCTAGAAAACGATATTGAAAATGATAGATATATTGAAATTTGAAATATCGTATTTTCACAATTTAATAATGATGGTGAAAACAATTACACCGAATTAGCAACAAAGAATATTGATACTGGCGCCGGTCTTGAAAGAATAACATCAATCTTGCAAGATGCTCCAACTAATTTTGATACTGACTTATTTTTACCAATAATTCATGCAACAGAAAAATTAACAAACAAACATTATGATATTAATAACTATTTTACAAAAGAACCAATTCAAATGAAAATTAACACCCAATTTAAAATAATTGCTGATCACATTAGAGCTTCAGTTAATGCAATAAGTGACGGTGTTAAACCTTCAAATATTGGTCGTGGTTATATTATTAGACGTTTAATAAGAAGAGCATATAGGGCAGGTATTTCTCTTGGCATAACTCAAAAAGCATTTTTATATGAACTTGTTCAAATAGTTAAAGATTCATTAATCTATGACATTGATGTTGAAGAAGTTAAAAAAGTTATTTTGGATGAAGAAAATCTTTTTGCTAAGACTATTAATGAAGGCGAAAAACTATTAAATCAAGAAATTGAAAAAAATGGTACTATCGATGTTAAAATAGCCTTTAAAATGTTTGACACATATGGCTTTCCTATTGAACTAACAAGAGAAATTCTACAAGAAAAAAACATTGACATCGATATGAATGAATTTAACGAATTTTTAAAGCAACACCAAGAAAAATCAAGAGGAAATATTGAAGCTGGAATGCAAAAGGTTATTAATTCACTTTCATTAATAACTGATAAGATATCTGAATTTGTTGGATATGATTACACTAAAACAACTTCAAAAATTTTATATTTATTAAATAGCGAAGAAAAAATAGATAATGCGGGCGAAGATGAAGTTTCTTATTTAATATTAGATAAAACTCCTTTCTATGCTACAAGTGGTGGCCAAAATCATGATGAAGGTTACATTTTACAAGGAAATAACAGAATTGAAGTTTTAGATGTATTTAAAGACAAATATTGAAATCATGTTCATGTTGTAAAAGGCAAAATAAATTCAAATGAACCAGTTCAATGTTTTGTTGATGAAGATAAACGTATTAATTTTGCAAGAAATCACTCTTCAACTCACTTGATGTTTAGTGCATTAAGAGAAACATATCCAAACGAAATTATCAAGCAATTAGGTTCGAATATTACATATGAACATTTTACATTTGATTTCAGTCTTGATCATAGGCCAACTAAAGAAGAGATAAAATTAATTGAACATAAAATGAGGCAATATATTGCTAGAGATGTAAAACGTAATTACATAATAACTTCGCTTAAAAAAGCAGAAGAAATGGGCGCATACATGACAATTGATGAATCAGAATATCATGATGCAAATTATATAAGAGTTGTTGAATTTGAAGGTATTACCAAAGATTTATGTGGTGGAACACACGTTCCTCATTCAGGTATAATTGAAGATTTTAAAATTGTTGCCGTTGATTCAAAAGGTACAGGAATATATAGACTTAGAGTAGTTACTTCATATGATTTAGTAAGAAAATATTTATTATCAGAAATTCAAGAAAAACAAAAAATATTGGATACATTAATTAGAAATAATCAAAAACTAAATGAAGGCTATAACTTTAAATATACAAAGGAAATTATTTCTAATAAGACATCTGATTTAGAAGAATTACTTGAAGAATATGATAAATGCGAAGAACAACTAAGAAATGATTATCGAACATTATTGAAACAAAAACAATCAGAAGATGTTGATTTAAGTTTGTATAATTATGAAACCATCAACGATAAAAAAGTATTCTTATTAATTAACATAGAACAATCATTAATTAAAAAAGTTTCAATCTCATTAAGAGAAGCAAATCCTGATTCAATTATTATTATCTTAAACAAGAGCAAACAAGATTCATATTATTTTGCAGTAGCATCTAAAAAATATGATTCAAATGCAATATTTAAAAACTTGGGCAAGGTTTTAAATATTCATGGCGGTGGAAATGCAATAATTTCTCAAGGTTCATTGGTTTCAAACTTGAAACAAGAAGAAATTATTGAAATTATTAAGAGCAATTTATGCGAATTTTAGCATTGGATTTAGGCACAAAAAGTTGTGGCTTTGCAATAAGTGATCCTAATAATATAATTGCAAGTGGACTTGAAAATTTTAGATTTGAATTGAACCATTTTAACTTAGTTATTGAAAAAGTGCTACATTATTTAAATAAAAGTGAATATCAAGGTTCAATTGGTACAATAGTTTTAGGATATCCCTTAAGAATGGATTTATCAAAAAGTCAACGAACATATATGGTCGAAAGATTTGGAGAAAGATTAAAAAAAGTTGTTGATATTCCAGTTGTATTTCAAGATGAAAGACAAAGCACTATTGGAGCTCAAGAAATATTGCAGAGCGCTGGATGAAACACTAAAAAATCTAAATCAAAGAAAGATTCACTAGCAGCGCAACTTATATTAGAAGATTATTTAAAAGGAATTAAATATGAATAAAAATATTATTAATTTAGATGTAGTTGATAGGCAATTGACTACAAGCGATGGCGAAAAACTTTATGTTATTTTTGATATTGAAGAAAACGGCGAACATTATCTTGTTTTAACAGATTATGACGCGATTATTTTTGCTAAAGAACAAGATCAAAATTTAATCGAAGTAACAGATGAAGGTGAAATTGACATTCTTGTTGATTTGACAATGGAATTTGCAGAAAATAATTTTGTTTTAGATAAAGATGGAAAATCAGATTTAATGAAGAAATTAATTGGCAACGATCAGGGTGAAAATGAAGCATAAGGGTTCAATATTTAGCGCTAATACAAAATTAGGTTTAATAATCTCTGTCGTCGTTGTATTTATTTTAATGTGTGTAATTTTGTTAATAACTATTTTAGCACGTAGACATTTCTTAAAAAGCACAATAACATCAAGCGAAGAACGAGAACTACAAAATTTAAAAACAAAGAATCCAAATTATGGAATTGTTTTGAGTGGCATTAAAAAATATTATGATAATGTTTTAGGCGACCATTTGATTGCGTTTTTAATTAATACAATATATTTGAATAATTTTCTAAACGTTAGTCTTATTGAAAAAAATGACTATGCTTCAATTTCAATAGCAGCGTTATCTATGGCTAATGTTTATTCAAAAAATCAATATTTAATTCAAAAGCAAGGTTCAAATATTCAAAACGAAAATCAGGATTTAGATCTATCTAGATTAAATTATGTTGATGAAATTTATAATCCTTCGGATATGATAATATCTTTATGCGATGATCTTTCAATAGAACAACAAATTAATTCAAATTTACCTTATTTAAGTGATAGAGGTATGCTAATTGTTTCATTTAAGACTATAAAAGACTTTAAAAATCAAAAAAATGTTATTTTAGATAATAATTTAAGATACGAAACTGTTAAAATTAGTAATAAAAATGTTATACTTTTAGCAAAAAACAATATGAATTCAAACAATGAAAATTCTAAGGAGCAACAATAATGGCTAATAATGATAATAAGATTTATTTAACAGAAGAAAGTTTAAAGCAATATCAAGAAAAATTAAAATATTTACAAGAAGTTGCAAGACCTCAAGTTATTGAAGAAATTAAAGAAGCAAGAAATCAAGGTGACTTGTCAGAAAACGCTGAATATGACTCAGCTCGTGATAAACAAGCTGCTATTGAAAATGAAATTTTAGAAATTCAACATATTCTTGAAAATGTTGAAATTATTAAATCAACATCAACTCATACAATTAGAATTGGTTCAACTGTAAAATTAATAAATATAAACACAAACGAAATAAGCGAAGTTAAAATTGTTGGTGCATTAGATGCCGATCCATTCAACAATAAAATTTCAAATACCTCACCGCTTGCAATTGCATTAAATGGCAAAAAAGTCGGACAAGTGATTGAAGTTGAAGCTCCTAAAAAATATAAAGTTAAAATTGAATCAATTTTATAATTTAAAAATATTTGCACTGCGCAAATATTTTTTATTGTTATTTTATAAAATAAAATATTTTATTTAATGAAACAATTTCACACATTAATTTATAATATCTAATAATGAAAAATGAAGTTGTTAAAAATAATTTAGGATATAGCGGAGATTTATTTGTATATCAAGATAAATCAATGTTTAATTACTCTGTTGATACTATTTTACTCGGCAATTTTGTATCAATAAACAGAAAAGTAAGAAATATTTTGGAAGTAGGCACGAATAATGGTGCCCTTTCTATTTTTATTGCTGCAAGAGATAAACGTTTGAACATTGATGCTATTGAAATCCAAAGCAAAGCTGTTGAACTTGCAAGAAAAAATATTATTTTAAACAAACTTGAAAATCAAATTAATCTTATTGAAGGCGATTTTCGGATGTGGGCTAATCAAAAGGCATACGACTGTGGCAATAAATTAGCCAAAAAATATGATTTAATTGTTGCCAATCCACCTTACTACAATGAAGATTATAATCAACAAAGAAAAAACTCAACTCTTGAACAAAAACTAGCAACTCATGAAATAAATTTAAATTTAGAACAATTAGTTGCTAATAGTGCAAGAATTATTGAACAAAAAGGATACTTAGCAATTGTTTTGCCAATGGCAAGATATATTGATCTAATTTGTTTGCTAAGAAAATACAATTTTGAGCCAAAGAGAATTCAATTAGTTTATCCAAGAATCACCGATCTGCCAAAATTCTGTTTAGTCGAAGCTAGATTTAATTCAGGTTGAGGAACATATTTTGAAAGCAATATTTATTTGCATAATGAAGATAAAAATGACCATAGTTATAGTCAAAAAGTAAAAGAATTATATAAACCAAAAAAATCTAATTTTTAAATATAAACATTGAAAGGAAAATATGAATAATAAAAAAACTTTTTATATTACCACACCAATTTATTATCCAAGTGGTAATCTTCACATAGGACATTTATTAACCACTACATTAGCTTCAGTATATAGAAATTACAAAAATCAACAAGGATATGAAACCTTGTTTATTACTGGAATTGATGAACATGGTCAAAAGATTCAAAAAAAAGCTCTTGAACAAAATATTACTCCAAAAGAATACGTAGATATTCAATCTCAAAAGTTCGTTGATTTATGAAAATTATTAAAAATTGATTATGATAAATATATTCGTACAACTGACGATTATCATATTGAAACAATTCAAAAAGTTTTTTATTTAATGGAACAAAAAGGCTATATTTATAAATCTAAATATGAAGGCTTATATTCAATTAGCGATGAAGAATTCTTAACTAAAACTCAAGCAATATCGAAGGACGGAAAATTTTACCACCCAATTTCAGGACATGAACTTCAAGAAGTAAAAGAAGAAAGTTATTTCTTTAATATGTCAAAATTTACTCCTTGAATAAAGTCATATTTCGAACAAAATCCTGATTTCATTACCAATAAAGCAACATATAAAGAATTAATGAATAATTTTATTAATAAAGGATTAGAGGATCTTTCGATAACAAGAGTTTCTTTTGACTGGGGAATTAAAATAGAAACTAAAGATGCTGAAAAGCAACACATTATTTATGTTTGATTAGATGCTTTATTTAACTATTTATCAGCATTGGGATACAATAATTACCTAGGCCAAGAGTTATATAATAAATTTTGGGTAAATGGTAATGAAAGAGTTCATTTATTAGCTAAAGAAATTTCAAGATTTCATGCGATATATTGACCTATATTTTTAAAATCTTTGGATCTTAATTTACCAACAAAATTAGTAATTCACTCATGAATTGTAACTCCTGAAGGAAAAATGTCAAAATCAAAAGGCAATGTTATTGAGCCAATTCCTTTAATTGAAAAATATGGGGCAGAACCCGTTAAGTATTTTTTTGCATCGCAAATAAATATTGACAATGATTTTAGTTTTAGCGAAGAATTACTTATTAATGTATTAAATGCTGATTTAGCAAATAATTTTGGTAATTTGGCCAATAGAACCTTTAAAATGGTAATGTTAAATTTTGAAAATGGTACAACTTATTGTTCAAACCAATTGAATTCATTAGATAAAAATGTTTATTCAGAAATAGAAAATGCATACAATGATTATAAAAAATATTTTGACAATTTTAATGCTGATAAGGCTTTAAAAAGGGCAATTGAATTAAGTAAGTTTTTAAATGAATATATTGATAAAAATGAACCTTGAAAATTAAAAGAAAATTTGCCAAGATTAAATGTGGTTTTAAATACATTGTTGAATGGAATTTATGCAGTTAATATGATGTTAAGCGTTGTTTTAAAAGAAAAATGTAGCATTTTATTAAACGATTTGCAATTGTCTGAATTCTCTGAAAAAATGTTGTTTAATTATTCTAAATTTGATAACAAAAAGTTTTCTAAACCTAATATTTTATTTGAAAGGATAAAACCAAATAATTAAATTATTGGTAAAATTAGTTCATGAAATTTATAGATGAAACAAATGTAATAGTAATAGCTGGCAAAGGTGGCGATGGCATAATTAGTTTCAGAAGAGAAGCTAATGTTGACAAAGGCGGTCCTGATGGTGGCGATGGTGGCGATGGTGGCGATGTTTATTTTGTAGGTGATCCTGGCCAAAATACACTTCTACCATTTTATTATCAAAATAAATTGGTCGCTGAATCAGGAGAAAATGGAAGGCCAAAAAATCAATATGGGGCTGATGGCCAAGATTTAATTGTAAAAGTTCCACTTGGAACAATGGTTTATAATGGCGATGATTTAATAGCCGATGTAGTTAGCCAAGAGAAATACTTAATAGCAAAAGGTGGACACGGTGGCAAAGGAAATCCTAGATTTAAATCTAGTACTAACACTGCTCCTAGAATATGTGAAAATGGTACACCTGGTCAAAAATTTGAACTTCACTTAGTTTTAAAGGTTTTAGCAGATGTTGGCTTTGTTGGAAAACCTAGTGCTGGCAAGAGCACTATTTTAGGCGAAATATCTAATGCAAAGCCTAAAATTGCAGATTATGATTTTACTACATTGGTTCCTCAACTAGGTTTGGTAAAATATTTTGACAACTCATACGTTGTAGCCGATTTACCAGGACTTATTGAAAATGCTCATTTGGGCAAGGGCCTTGGAATTCAATTTTTAAAGCATATTGAAAGATGTAAAATTATTGCTCATGTTATTGATTTTGGTAGCGAAGAAAAGAATCCAATAGATGATTATGAAATAATTAATAATGAATTAAAATCATATAATTTGTTGCTTGAAAAATTACCACAAGTAGTAATAGCAAACAAAAAAGATCTACCGGCTTTTTCACTAAACTTATCAAAATTTAAAGAAAAATATCCAAATATAAAAGTATTTGAATATTCAGCATTAATGGCTGAAAATTTAGATGAATTAAAAGGATGCTTGTTTGAAACATTGCAATCTGCTAAAGATTCAATCGCTGTTCAACAAATTCATGAAAGTCAAAACATTGTAAAAATAACATTAAATAAAAAACCTATTCAAATTAAAAAAATTGATAATGAAACATATGAATTGTTTGGTGATGATATAGATAATATATATAATAAGATACCAGTGATTTCATTAGATAATTTATGAAGATTTAATGCCAAATTAAAAGCATTAGGTGTATTTGAATTAATAAAACAAAATAATATTAAAGATGGTTCTACAATAAAAATTCATGATTATGAATTTATTTGGAATGCTGAAGAATTTTAAAATTTGTCAAGATTTTATTATTTTTTAAAAATTGTATATTACTATGTAATAAAAAAAATTTTAATTTTAAAACTTTTAAAAAAATAATATATAATAATTAAGCATTAAGCAAAAGCAATAAGTATTTATTTATAAACAACAATTGTGGGGAAGTAGCTCAGCTTGGTAGAGCACTTGACTTGGGCTCAAGCGGTCGCAGGTTCGAATCCTGTCTTCCTCACCATTTATGGGGGGGTAGCTCACCTGGCCAGAGCGCCTGCCTTGCACGCAGGAGGTAGAGGGTTCGAGTCCCTTCCTCTCCACCATTATGGCACTGTAGCTCAGTCGGTTAGAGCGTCCGGTTCATACCCGGAAGGTCAAGAGTTCGATTCTCTTCGGTGCTACCAAATGCAACAAGCCTAACGGGCTACGGACCCATAGCTCAATGGTTAGAGCAACCGGCTCATAACCGGTCGGTTACAGGTTCGAGTCCTGTTGGGTCCACCATTGGGCAATTACCCAAGAGGCTGAAGGGAGCAGTCTTGAAAACTGCCAGGGGCTTTACGGCCCGCGGGGGTTCAAATCCCTCATTGCCCGCCATTATATAGCGGAGTAGAGCAGGCGGCAGCTCGTTGGGCTCATAACCCAAAGGTCATTGGTTCAAATCCAATCTCCGCAACCATGGTCCAGTGGTAAAGTGGTTTAATACGCTTCCCTGTCACGGAAGAGATCGCGAGTTCAATTCTCGTCTGGACCGCCATGGCTCTGTAGCTCAGTTGGTAGAGCAACGGACTGAAGCTCCGTGTGTCACTGGTTCGATTCCTGTCGGAGCCACCATTTCAGAGATGACTAACACCTTAGGGTGTTTTTTATTTTTTATGTTTAATAAAAGATAAAAAAAGTAAAGTATAATAATACATGTTATGAATATTAATAGTATAAAAATTGCAATTCAATTATTTGGAATTTTTGGTTCAATTGTAACGATTTGTTTAGGAATTCCACAATTAATTAGATTAAAAAAAACAAAAAACACGGGCAAAGTTAGTTTTGCAGCATTTTGAGTTTTTTATTATGGAATTTTAGCGTGACTAGTTTTAGGAGTATTTACTTCTAGCGAAAATTCATGATATTTATTCTTATCAAACTTTGCATGTGTTACAATAACATCCTTTACAGTATTTTACTTGTATTGATATTATGAAAAATTAACTAAAAAAATGTTAATAAGGGCTATTGTTGGAATAAGCATTAATCAAGTTATTTCATTAATTTTCTTGTGCCTATTTTTTGTTACGGTATATCAAAAGTTGAAATTTGGTAAATTTTATCCCGATAATTCAATTATTCCTGTTCTTCCGGGTAATGCGCAACTTGCATTTGGACTAATATGTCCCGCCCTTACAAGCGCAGCATTTTTCCCTGGATTAATTGCTAGTTTAATTAAAAAAGAAGAAATAAGTTTAAGCCCATGAATGGCTCTTGTTTATATGCTAAATAATACTTTTTGAATATTGTATTTCTCATTAAATATTGTTTATTCGAAGTTTGGCGTGGATCAAGCAGCACTTAATAGTATGCCAGGATTTATCGGGGGACTAGTTTGACAATTACTAGCATTAGGAATGTTTGCTTTCCAATTTGGAGTTACATTACACTTTGATATTAAGCACAAAAAACAACAAAAGCAAGCAATGGTTAACGTTATCAATCAAAATTAATCAAATTAAAAGTAGCAAATTACTGATTGCTACTTTTAATTTCATCATTTGCAACTTTAGAATATGCTCTAATTAATTTGCTAGCGCCTAATTTTATGCCACCAAAATATCTAACTACAAACAACACACAATTATTTAATTTATTTCTTTCTAGTAAAAAATATAAAGGTTTGCCTGCTGTTCCTGATGGTTCACCATCATCACTCATTCCACCACATTGAACGCCATTTTCAATAACTAAATAAGCATAAACTATATGCCTTGCTTTTTGATGTTGATTTTTTAATTGCTCTTTTAAAAGTTTCACATCATCTTTACTTTTGATATTAAATTTATAAGAAATAAATTTAGACTTTTTAATTTCTAAAATTGCCATAGTTTTATTATAATAAAAATTATTGTATATAATTACTAACATGTTAAAAATTATTTCAAATTATTCAGACAGAATAGATAAATATGTTGCTAATAATAGCGAGATTTCAAGAAACGATATTAAGGAATTAATTGAAAATGGCGCAGTATTTGTAAATTCAATAAAGATAAATAAACCTAAATTCGTTGTTAAAGAAGGCGATGAAATTTTAATTGAAAGATTATTAGATAAACAAATAAAGGTTGAACCTCAAAACATTCATTTAGATATAGTGTATGAATGCGATGATTATTTAATAATTAATAAGCCATCAGGATTAGTTGTTCATCCGGCTCCTGGACATCATGATAATACATTAGTCAATGCTTTGATGTATCATTTTAAAAATAATTTAAGTGATGTTAATGGTTTATTACGTATGGGGGTTGTTCACAGAATCGATAAAGATACTAGCGGTTTATTATTAATTGCTAAAAATAATAAAGCGCATAACTACTTTGCTTCGCTTTTAAAAACACATGAAATTAAAAGGAGTTATTACGCTATTGTTGAAGGCAAGATTGCAAATGAAGAATTACATATTGAACTTCCAATAGGTAGAGATACTAAAAATCGCCAAAAATATGCTGTTAGTGAAACAAATTCAAAACCCGCTTATACAATAGTAAATGTTGTCAAATATTTTAAAATGAATAATAAAGATTTTTCGTTAGTTAAATGTAATTTAAAAACTGGTCGTACTCATCAAATTAGAGTTCACTTAGCATATATTAAACATCCTGTTTATGGCGATCCAATTTATGGTCACAAAATTGATGATTTCAATCAAAGGCTTCATGCATTTGCTTTAGATTTTGTTGATTTAAATGGAAACAATAAACATTTTGAAGCAGAAATGCCTGAAATTATGAAAAAAGAAATAACTGGCGAATAAAAAAACCTAAATTAAATTGCAATTTAAAATATAATTATTAAATATAAAAGGAGTTTTAATGAAATCAATAAACATAAATGAATATAATTCTACAAATGAATTATATACAAAGAACAAAAAAGTTTTTTGGGCTTGATTCATTGCATGAAGCGTTTTATTTGCAACAATTTTAATATTTGTAATTGCTTCTTTTATTCAATTTGCAGTTGAAAAACAAAACTATTTGAATAAATATGCTGAACTTTTTGGCCAAATCCAAAAATTAAATCCAAATTTTCAAGGAGATGTATTCGCACTAGCCAATAATGAATATGTCAAATCTTTGGCAATGTCAGGCGTTTTACTTGTAATTTTAATTGCGGTTTATGTTTGACATATTGTTTCCATAGCATTGGTAGCCAAAAATAAGGATTTTTCTGCTTATTCAAGAATTTTAACTACATTATATTTATTTGTAATTATTTATGAAATAATAACATTAATCTTTTCTGGATTTCAATCATTTTCGTTAGCAATTTGACAAGTAAATAAAATTTTAAATTTAGTGGCAACATTGTTATATATAGTTTCATATTTTGCTGTGTTTTTAAAATGTTCTTATGTTGCAAAATTATTTTTAATTTTAAAGAGAAATTTAGCATTTAAGGCATTAAACAACTCGGGCTCAATGGATGATATTTTAAGATCATATTTCTACGGTCAAGAATCTCCTGAACCAACACTAAATACCAATAATACATTTGATTCAAATCAAAAAACCGAAGAAAACAAATCTGAAGATTTAAACAAAGAAGAAGATTCAAGATCAATTGAATATCGTAGTAAATTAGAACAATTAAATGATGAACAATTGCAAAATATGGCTAAAAAATTAAATATTTTTGGAATTGAAACATTTTCAAGAGAACAATTGATTTCAAAAATTAGCGATATTTTTGAACAATCAGCCAAGTCTCAAAATCAACAATCAAAACCTGATAATTCCGATGAAGACAACAACGATGGAAGTCAAACCAGCAATTAATTTTTTGTAATTTTATAATTACTATTTAACAAGGAAAAAACAATGAATAATCAAACATCATTAAAACAACATATTTTTACTTTTTTTTCACAAAATTCTCCTGCTAGTCCAATAAAAAATGGTGATCAAAAATTATGAATTCCTGCATTAGTTTGCATTATATTAATTTTATTTTCTCTATATCTAGCAATAAAATTTTTTGTTAATTTTAAACGTAGCATTGCTAAAACTAAATCTATTTTAAAAGACGATCTTGTTAGTGCCTATATACAAGGCTTTAACATTAAATCCTCAGCAGTTTTTAATTTTATATTAGCTAACATAATTTCTTTAGTTTCAATTATCATTTCTATAATATCAATAGTTAAACATTTTTCAGCAAGCTCAACGTTAGGTAAGTCTTCGTTTCTAGCGTTGTTTATAGCAGTGATTGCGCTTTCAATTGTTTTAATAATATCAATTAATTTATTACTATTAGCAATTTATTTTGTTTCATTTAAAGAAGCTAAGTTTGTTAATAAAAAAAATTTAGAAGATGAGCTTATTTCTTTGAAGTATAAAAAATTCAAAGAATTAAATGATTTAAATGCTCCTGAAAATATTAAACTGGATATCAAAGCAATGGAATCACACAACCCATTAGCCCCTAGAGTGTTAGCAAAATTTATTGATTTTTATAACAAAATGTCAAGTATTGAATTATTTAAACGTTATAAGAATTATTTAAATCAAACATTCAAAATTAGATATTTTAATGAATCTCTTGAAGTAATTGAAGGAAAAAATGAAATGGAAGATAATCTTGAAAAATATCTAGACAACATAAATAATAAAGATCCTGAATTGCAAAAAAATTCTCTAATTTTAAAAGAAATTGCTTGAATGAATGATATGGATAAAAAGGTAAAAATTATTAGAGAAGCAGATAAAAATATAACCATGTCTAAAAAAGAATTTCAAGAAAAATACGATGAATATGTTTATTTGCAAAGATCACAAGATCCTGCATATCAACAAACTCAAAAGAATACATGACTTACATATCGTGATTCTGATATTGAAGATCTATTTTATAACACAAATACATCAGTTATTTATACCATTGATAACGGCAAGACAATTTTAGAAAAACCAATGAATGAATTTTTAAATGAATATGGACAATATTTAGAAACTAAATTTTTTGCATCAATTAAAGAAAATGAAGAACTTAAATAAGAGATTGCAATGGCTATAAAACACAAAAGAATTTTATTTTTATCAACATTAGCATTACCGGTAGTAGTTGTACCTGCAACTATGGTTTCGTGTTCAAAAAAAGATATTGAAGAAATAAAATTAGATATGATCACAATAGCAAAAAATCAAATGCTAAATTATTTACAAAAATTTGAATCATTTTTTAAAAATGATGATGATCCTGATGTTAAAAATTTAGAAGTAGAATTTAATAAAGTTATAATTTCGTTAAAAAAAATGGATATAACACCGGATTTTAATATGGACCAATTTATTCAAGAAATGACTAAAAAATTCAGCGATGTAGCAAATAATTTTTTGAATTTAAAATACAATAAAGCCAAATTGACAAGTGAATATTATATTTCAAAAGTAAAAGTTCAGTTAATTTTAGAACAATTTAAAAATCCCGGTTTGTTTCCAGAAAAAAATGAATTTGCCAAGATTTTAGAGCAAGAATTATCAAAAGTTTCCGATCCAACTATTAAAACCTCTGAAGAAGAGTTAAAAAATTCAATTAGATTTTTAGATGATTTAAACAAAAAATATGATGAAATTTTAAAAAAGCAAGTAATGATTTTAAAATAAAATTTCTTGCTTTTTTTATTTTTTGTTTTTATTTAAAAAAAGTTTAATTTTTTTTAAAAACACCGGGTTAGTTGTTGTATAATTGTACAGTATTTATCTATTAAAATACCAAAGACCATATAAAAATACAATATATAATTTAAATTTTAGAAAGGAAAAAATATGCCTACAGTTGCACAATTAGTAAAACAAGGCCGTAAAGATAAAACAACAAAATCTAAAGCTCCCGCTTTAGGAAAAATGTATAACTCTTTACAAAAAAAAGAAACTGCTATGCCCGCACCATTTAAGCGTGGCGTATGTACTCGTGTGGCAACAATGACACCTAAGAAACCTAACTCAGCTATTAGAAAATATGCCCGTGTTAGATTATCAAATGGTCAAGAAGTTACTGCCTATATTCCCGGTGAAGGACACAATTTACAAGAACACAGTGTTGTTTTAATTCGTGGAGGTAAGGTTAAAGATTTACCCGGAGTTAGATACACAATCGTTCGTGGAACACAAGATGCTGCTGGAGTTAATAACAGAAAGCAAGCTCGTTCTATTTATGGAACTAAAAAACCAAAAAGTAATTAATTATAGTTAGAAATTAGAAAGGAAAATCATATGTCAAGAAAACATAAAGCGCCTGTTAGAGATGTCTTAGCAGATCCAGTTTTTAACTCTAAGATAATTACTAAATTAGTAAATACCATTATGTTAGATGGTAAAAAATCTATTGCTGAAAACATTTTATACAGTGCATTTGATATTATTAAAACAAAAACAGGAAAAGAACCAGTTGAAGTATTTAATCTAGCATTAGAAAATGTTAGTCCACAACTAGAAGTTAGATCAAGAAGAGTTGGTGGTTCAAACTACCAAGTTCCTTGTGAAGTTTCTGCAAAAAGAAAACAAACTTTAGCATTAAGATGAATTATTCAATATGCAAGATTAAGAAACGATAAAACTATGGAAGAAAAGTTAGCAAATGAATTAATAGATGCCTCAAACAAAGTTGGTGGCGCTATTAAGAAACGTGAAGATACCCATAAAATGGCTGAATCTAACAAAGCATTTGCTCACTTTAGATGATAATAGGTTGGTTGTATGTCTCGTGAATATAAATTAGAAGATTACCGCAATATCGGAATTATGGCTCACATTGATGCCGGAAAAACCACAACTACTGAACGTGTGTTATATCACACAGGAAAAATTCATAAAATTGGTGAAACACACGAAGGTGCTTCACAAATGGACTGAATGGTTCAAGAACAAGAAAGAGGTATAACTATTACCTCAGCTGCTACTACAGCTTATTGAAAAGGAAAAAGATTAAACATCATCGATACCCCAGGACACGTTGATTTTACTATCGAAGTTGAACGTTCGTTAAGAGTATTGGATGGTGCAGTTGCTGTTTTGGACGCCCAAAGTGGTGTTGAACCTCAAACAGAAACAGTTTGAAGACAAGCAACAAATTATCATGTTCCAAGAATAGTTTATGTTAATAAAATGGACAAGATGGGTGCTAACTTTAAAGCATCTGTTGAATCACTTCATAAATTACTTGGTGCTAATGCTCACGCTATTCAATTAAATATTGGTGAAGAAAACCATTTTGTTGGAATCATTGATTTAGTTGAAATGAAAGCATATTCATATGATGGCGAAGTTGATGAAAACGCTAAAGAAATTGCTATTCCAGAACATTTAAAAGATGAAGCTCATATTATGAGGGCTGAATTAGCTGAATCACTTGTTGATTATGATGAAGAACTTATGACTTTATTATTAGAAGAAAAAGAAGTTCCAGTTGATATTTTAAAAAGAGCAATTAGAACTGCTACAATTTCTTCTGAATATTTCCCAGTTGTTTGTGGAACATCATTTAAAAACAAGGGTGTTAAGTTAATGCTTGATGCAATTGTTGATTATTTACCAAGTCCTTTAGATATTCCTCCAATGAAGGCATATAAAGGCGAATCAGAAATTCATATCCCTGCCTCAGATGATGAATTTTTCTCAGCTTTAGCATTCAAAGTTATGAATGACCCATTTGTTGGTAACCTAACATACTTTAGAGTTTACTCAGGAATTGTTTCAAAAGGTTCTTATGTATTCAATTCAACAAAAGGTGAAAAAGAAAGACTAAGTAGAATTCTATTGATGCATGCTAATAGTCGTACAGACATTGACGAAGTTAGAACAGGAGATATTGCTGCAGCAGTTGGTTTGAAAGTAACAACCACTGGAGATACATTGATTGATGAAAAACAAAAAGATGTTGTATTGGAAAATATGAATTTCCCTGAACCAGTTATATCTCAAGCTATTGAACCAAAGACCAAAGATGCTTCTGAAAAATTATCATTAGCATTGCAAAGATTGGCCGCTGAAGACCCAACATTTAAATACTACACCGATGAAGAAACAGGACAAACAATTATTGCAGGTATGGGTGAACTTCACCTTGACATTATTGTTGACCGTCTAAGACGTGAATTTAAAGTTGAAGTAACAGTTGGTGCCCCACAAGTTTCATATCGTGAAACCATTACAAAGACTGCCGATGTTGAAGGTATGCACAAAAAGCAATCTGGTGGTAAAGGTCAATATGGACACGTTTGAATTAAATATGAACCAAACCCAGATGGAGGATTTGTATTTATTGACAAAATCGTTGGTGGTAAGATTCCTAAAGAATATATAAAATCTATTGAAAAGGGACTACGCGAAAAAATGGCTATTGGTATTTTAGCAGGATACCCTATGATAGATATCAAAGCTACTTTATTTGATGGATCATACCACGAAGTTGACTCGTCTGAATTAGCATATAAAATTGCTGCTTCTAAATCACTTACAAAAGGCCGTGAAGCCTTGGGAACAGTATTACTAGAACCTATTATGGATGTTGCTGTTGTTGTTCCTGAAGACTTCTTTGGAGATGTTATGGGTGATATTTCTAGAAGAAGAGGACAAGTTAGAGATAACGAAACAAGAAACGATGGAGCTCATGTTATTAAAGCATATATTCCATTGAGTGAAATGTTTGGTTATGCAACTCAATTACGTTCAATGACTACAGGTCGTGGAACATACCAAATGTGATTCGATCACTATGAAAAATTACCACGTAATTTATCAGAAGAAATTATTAAAAAACGTGGCGGAAAATTAGTAGCTGACGAAGATTAATAATATTAAAAAAGATGCTGCATTTTGTAGTATCTTTTTTAATAACTAACTTTAAAAAATTTATATAATTGTTTTATGCCTTCAATTAAAAAAAATACTAATTTAAAAGATTATCAATTAAATAATAATAAAAAATTGCTAAAAGCAAGACATCGCCTAACGTTTTGATTTGGTATAGTGATTTTATGTTTATTTGTTATTACTTTTGTATGTTCATGAATTAGCGATGGACATGAATTAAAAGTTGCTTTTTCTAAATTGAGTCAAGAAACTCGAGATAAATTAATTTTAGCTAATGCCTTGCCTTCTGTTTCGGGTGCAATGTTACAAGTTAGATTTACATTTACCTATATTAGTAACATTTTAATAGGTTTTGCATTGATATTTTATGGTTTACTTCCTAATTATCTATGGACAAAAAGAGGATTTTTTTTGGCAACTGTTTATATAAATATAACATTCTTTACTTTTTGAGGTTTAATAATACCTTTTGTATTCATTAATCCAAATATATGATATGAAATTCCTTGGAATTGAAAAGTTTTAACTGTTCCAGTTCATTTAATAAATCCCGTTATAGGAACAACATTTTTTATAATAAATAGACGAGAATTAGTAGTCTCTCATAAGACAATGCTTTTAAGTTCAATTTTTATGTTGGGATATTATTTATTTGCTGTCTTAATTTTTTGAATTGGTGTGCAAGTAACTAATTTATTTTTAATTGAACAATTTAATAAAAAAACAACTGGACTTGATATTTTTACAAAAACTAAGGTGGCAATCTATCCCTTTTTAAATTTAAAATATCCTCTTGGTTATCATGGCAATAATTTGTTTTATAAAATTCTATTAAATTTATTTATTCCTATATTTGGAATTATTGTATGTATATCTGTTGCTTATTTTTTAAAATTTATTTGTAAGATTCAAACGTATTCAAAAAAAGATTTTGAAAATCCTGCGTTTTTAAGAGAAAATAAGCTCTTAATAAAATCAATAAAATCACAAATTTTTAAGTAATAAGGAAAATCATGACGGCTTTTAATTTAAAAAAATATTTTGAGTTACAAACAATTGAAATTAAAAATAGAATTAAGCAATTTAACAATAAACTATATTTAGAATTTGGTGGCAAAATTTTTGACGATTTACATGCCTCAAGGGTTTTACCCGGCTTTTTAAGCGATAGCAAATTACAATTATTGTTGAAATTAAAAAACGAAGCAGAAATTGTAGTTGTAATTTCTGCTAATGATATTGAAAACAATAAATTGCGTGGTGATCTAGGAATCAAATATGAAGACGATGTATTGAGACTAATTGATAACTTTAAAAGTTACGATTTAATTGTAAATAATGTAGTTATTACCCAATATCAACCAACATTTAGAGTTAATAAACTGCAAAACATTCTTAGTGAACATAATATCAATGTCGTTAAACACTATAAGATTGACGATTATCCTAATAATTTAGATTTAATAGTTTCAAAAAATGGATATGGCAAAAATGAAACTATAAAAACTCAAAAGCCATTAATAGTAATTACTGCCCCTGGACCAGGTTCTGGAAAATTAGCAGTTGCATTAAGCGAACTTTATCATGATGCAATAAATAATATAGATTCTGGTTATGCAAAATTTGAAACATTTCCCGTTTGAAATTTAGCAGTTGATCATCCATTGAATTTAGCTTATGAAGCTGCAACAACAGATTTAAATGATCAAAACAAAATCGATCCTTATTATTTAAAAAGTCGCAATAAAATAGCAACAAATTATAACCGTGATATTCAAGCATTTCCAATTTTAAGTCAAATAATGAACAAAATTCAAAAGCGTAATATTTATGATTCACCAACATCAATGGGAGTTAATATGGTTGGATTTTGTTTTAATAATGAAGATGAAATTAAACAAGCGGCAAATAAAGAAATAATTCGTCGCTACTATCAAAGTGCTGTTGCTTTTAAAAAAGGTATGGTTTCTAAATCAGTATTAATAAGAAATGCAAACTTGATATCTAAAAATAATATTGATTTTTCAATTAGACCTTGCATTGAAATTGCTAACAAATTAGCAAATCAATTGAATATGAATATCACAACTTTTACCTTAAACAACAATATAAATATTACAGGTAAGACATCAATGCTCCTTGGTTCATCATCAGCTGCGTTATTAAATGCATTAAAATATTTAGCAAATATTCCTAAAGAAATCCATTTACTATCAAAAGAAGCAATTGAATTAGTTCAAAATCTTAAAGTAAATGTTTTTAAAAGTAAAAATCCCCGCTTACATATGAATGAAACTTTAATAATTTTAGCAATGTCTGCTAAGAGCAATCAATATGCAAAATTAGCCTATGATCAACTAAAAAATTTAGCAGGTATCGAAGCACATTGCAGCGCAATACTTTCTATGCAAGATATTGAAACATACAATCACTTAAATATTAACATTACTCAAGAACCAAAATACGATAATGACACCCTATATCATAAATAGAATATTTAAAAAACATCAAGTTGTATCATTAACCGCTTGATGTTTTTTAAATATTATTTTATATGAAAATCTAAATCAAAATCGTTTTCTATTGCTTTACATTCGATATTAAAGGCAGCAAAAATTTTATTTGAAGCCTTAAAATCATCTGAATCAGGCTTATATGCAACTGCATAAACAACTTTAGAAATCTTAGATTGGACAATTAGCTTAGCACAGTGGTAGCATGGTGAATGTGTTACGTATAATATAGATCCTGGTATAATCTTTGAATTTGTAATATTTGCATTTATTATTGCATTTGCTTCGGCATGGACAACGTAAGTATATTTGGAATTTAAGACATCGTTTTGGGTCGTAGGTCTATTTCAAGTGAATAAATCATCATTGTTAACTTCTTTATTTAAATAACTTGTTGGCATACCATTATATCCTAGCGATATTACATAATTATCTGGACTTACAATACAAGCTCCTACTTTAGTTAAAGGATCTTTCGAACGTAATGCACTTAATTTGGCAAGTGACATAAAATAAATATCTCATTTTAAATTATCTTTATGGTTGTTAAAAATTAATTTTCTTGTTTTATTATTCATAATAAAATATTTTAACATAATAAAGACAAAAACAGGCCCCTCAAGTCCTGTTTTTAATTATTCAGTGAAATAAGCTATCAGCTCTGTTAAGACATTAACTATGTTAATCCAATTTACTTGAAATTGAATTGCTATAATTATAATAAAAAATTAAAAAAATTTTTATTTTTTGCAATATTTTGTTTTTAATTGCTAATTAATGCAAAAAATACACCTTAATGTTAAAAAAAGTATACAAATTTAAACATAAAAGCTTAAATTGTTGTAAAATAAGTCTATAAAAAAAAGAAGGAAAAAGAAAACATGAATCAGAGTTTGAAAAACCAAAGAAACTTAAAGAATATGTTTAAACCAGCTATGTCTGGCTTTTTCGCTATATCAATTATTAGCTTTATTATGAAGAATGTTATGAATTGCGTTATGTCTAAAAACATCAACTATATTATGACAAACATCATTTGAGGAGCGAATAATGACTATCAATTTTTCAACTATAAATAATTTTTTTAACAATCAGTATATTAAGGCAAAAAATAATCTTAAAAACTGAATAATGGCTTTTAGAGGTTCATAGCTTCGTTTAATATTTTTATATTTTGTATTTAAGAATATAAAAAATATTAAAAACATAAATTTATTAAACTAGCTAATATTAATATCAAAAGCCAAATATTTCAAATATTAAGTTTATTATTCAATTATTTAATAAAATTACTAACATTTTTAATTACTTAATGACCTTTAAAATTAAAAATGTTGGTATAAATTACAATAACATTCTTTAAACAATTATTGTTTAAATATTTACAAACAAGATATTTGTATATCAACAAAGATATGCAAAACAATATATAAAAATTTAAAATTTTAAGGATAAAAATGAAAACTAAATCAAAATTATGATTACTTGGTCTTGGGTCCTTACTAGCAGTAGGAGTACCAGGATTAGTAGCCGCTTCATGTAAAATAGACCCGGCATATGTAAAAGGCGAATTCGTATATGAATTTAACGCAGTAAACAAAGAACCTATATTTGATGCAGACGTTTCAAGTTCATATGGTCAATATATAAACTCATTACCTGCAACATATACAGGTAGTGAACTTGTTAGAAAACAAGCCTTAACTCAAACAAAAACTATAACAATAACAGAAAAAGGCACTAAAAAAACCACTACCTATTTAGTAAAACCTACTTTTGCTAAATATAGATTAGAATTAGCAGAAGCTATTGTTTTAACTGAAAAAGGAACTAATAAACAATATGTATTTGATTCAGATGAAATTCCAAGTGAATCTGAATATCCACAACCAACTAAAACTGTAACAGATGTTACCGGCAAAGAATACAAAGTTTATGAAAAAACAAACGTTTTTGTTAAAACAAAAGGTGACAAGAACGTTGAATCAAGATCAATTAATAGTGCATTCTTTTTAGACAAATTAGCTAATGCCTCAAAATTACAATTTGTTATTAGAAAAGGTGTTAAATGAGTTGATCACGACGGAAAAGACACTGGATATGAAGTTAAAGCAAAAGACTTCTGATATTCATGACTAAGAACATATTCAAGAAACTTAGACTATCGTTTAAGCACCGGTGGATCAAAAGAACTAGACGATGCATTAAAATCAAAATTGGCTGAAGAAAATTCACCAGTTTATGGTGAACAATGATCATTTGGTAACATATATACTTATGCATTGTTCGGCATTGATGCTAATAAATTATTTGAAAAAGATAAATTTATTCAAAAAGTATCAGCTGAAGGTAATTTAAAAGATAGAGAAGCAATTACTTTTGAATCAATTACTGGAAAAGAAAAAGATGCCAACATTTCAAGCTTCTTTGAAAGTCAATTTGCATCAGGAACATATGACTTTATGCCTGCTCCATCTGACTACATTGATGCATTAAATGCTAAAAATGAACAAGTTGTTTTAAAATCAAACGGTATTGCTTTATCAAAATCTGAAACAGAAGCATATCGTAACGCTTTAAATTCAATGGATAAAGATAGCTTACAATACAAAATTGGTCAATACTGATATGGAACATCTGCTAAATCTACATTGTACTCAGGTTACTACTACAACAAAGGTATAGAAAACCAAATCCAAAAATTACCAAAGAATACACTATACTGAGATAAGAAATGAGTTGAAGATGATTCAACAATCAAAACAATTGTTTTCAACTTCCAATCAAAAGCAGTTGAACCCGCATTATACAACCAAACAGCATTTAGAAAATATAGCCAAGGAACAATTAGCCAAATTGGTTTTAGTAGTTTAACAGAACCACAAAAGAAAGAAGTTTCTTCAGAACCAGAAAAATTTGGATTGAAATTAAATGAACGTTTGAATGTCCAAACTTCAATCTTTGGATTTGTTAGAACACCATTTATTTCTTCAAAATTAGACAAAAATTACAAATTCAATGAAAACTTTGCTAAATTAGTATACGGATCTTCATTAGCTGATATTGACAGTGGAAAAGCAGACACTTCAAAATCATACCTATTTGGTAAAGGTTTAAGCTTCAGAACACTTCTATATGCAGCAGTAAACTGAGATACATATGCAGCTCAAGCATCTTCTGGCCAAGCAACTCACTGACTTGCAAAAGTCCCAGAAGGATCAAATTTAGGTGGTTCAGATCAAGGCACTGCAAAATTGAACACTATTACAGACTTTAGAGACCAAGCTAACTCACTATTTGCAATTGACAAAGATGGAAACAAAGTTGATTTCGGTGCTGAATTAGGTAAGGAATTAAGTCCTTCTGAAAACACCAAAGCTTCAGAAACTGAATCATCAATTTCTGAAAAGATGAAATCAGCAGGATTTAAAGTTCTTAAAGAAAAAATGAAAGAATTACTAGATCAATTCTTTAAAGAACAAAACATTACCGATCCTGAAAAACAAACAATTGAATTTGAAGTAGGATTTAGATATTTAAATGCTCCACAACTATACATTAATGCATGAGAAAAAGTTAAAAACGTTTTTTCTGAACTAGATCCAAGAATTAAAGTTACAGTTAAGAGCTTTGATGACCCGCGTGATCCAAACTTCAACGCATATAGATTTGATGGTGTGACAGGTGAAAACTTATTAGCATGATCAGCAGACTATAACGGTGTTGCTTCAACATACGACGGACTTTCATGAGGAGCTGCATTAGTTCCAACATTGGTAAAGGTTGCACACCAAACAGGCGCTGAAAAAGCTAAATTTGAAAAAGCCTTCCCAGAAATTGCTAAATTAGCTGATAAATTGTTAAAATATGCAGAAACAAACAAACCAAATTTATCATTGGATTTTGATAAAATTGATCAAATTGACAATAGATATTTAAATTCAAACTTACCTTCAATTTTAGCATCATATAAATTAGAACAAGATACCAATACTAAAAAATATGTAATTGCTAAAAAAACTGTTAAAGGTTCAAAACCAAAACCAATCGAATTCGATTTTGGAAAAGACAGTTCAGGAAAATCAAAAGAACCAACCGATCTATATCAATGATCTGCAATATTCTGAGCAAGCACATTTAAGAATTTTACAAATGAAGAAATTTCTAAGATTGTAGAAGAATTTACAACATTTGTAACCTTAGGATATACTAGTGACGTGTTTGTTGGCCGTGATAAATTTGTTAAAACTTTAATAAACAAACACTACGTTTCACCAACTATTGCAGGATCAGCAGTTGATTCATACCAAGATTGAAGAATAATCAAAGATACTAAAAAATAATTAATTCAATAAAAAATGACAAAATATATTTTACAAAGAATAGCGTTTGCTATTCTTACTCTATTTATTATCTCTATTTTCGTTTATGTGCTAGTTGCTGCATTTGGAAACAATCCAGTTAATGACTTAGCACAAGCACAATTTCAAGCAAGCAAAAAATCAGGATTAACATATGCTGAAATATTGCATAAACTTGAAATTGAAAACGGATTAAGATATAAAGATAACCCGGACATTTTAGTTCCTATAATTGTTAGATATGGCAATTATATGAAAAACATTTTTACGAAGGGCGATTTTGGATTTTTATATAATCCAGCTAACAACCCAAACTCTGCTTTATATACAAATATGGCAAAACTATTCTTTACCCCCCTACAATACTCATTAATAATCACTGTTCCAACATTCTTTATTAGTTCAATATTGGGTATTGCAATAGGTGTCATTGCTGGATATAAGCGTGGAAAATGATTTGATGTGTCTTCAAATGTTTTTGTGTTAATATTTGTTGCTATACCATCATTTATATTGGCTCCTATTGTAATAACTATTTCAGTCGCATTAGGAATTTCTAGCGTTGTGCCTAGACCTGGTGAACAATCATTTGGAGTAATGTTTGTTTCTTATTTACCTCCTATATTTGTTATGTCAATAACCTCATTAGCAGGCTATGTAACATACACAAGAAATCAAGTTATTACAGTATTAACATCAAATTTCGTTTTAATAGCTAAAACAAAAGGATTGAGTTCTTCACAAATTTTCTTTAAATATGTTTTAAGAAATATTTCAATTCCTTTATTTAGCTTAGTTTTTGGGTCATTTATTGGTCTATTATCAGGATCAATAATTATTGAACAATATTGACAAGTACCAGGAACATCACAAATAATAGTTAATGCCTTCCCTACCGGAGAAATTAATGTTGTTATGTTCTCTACATTATTCTTTACATTTATATCGCTAATTGCCGATATTATTATTGACGTTTCATATGCAATATTAGATCCTAAGATTAAATATTCTACTAATAGCAAGAGAAACTATTGATTATTTTTCTTGGCATATAGAGAACGTTCTAAACTTGCAAAAGATTTGTTTAAATCACAATTAATAAACAAAGCAAGTCAAAATACAACTGTTAAAGGAGATAACTAATATGAATATAAATGAAGTTAAAGCCTTTAACAAAGAATATAAAATTAATACTGAATTAGAGAAAAAATTTAAATTTGTTGCACCAGAAGATAGAATAGTAATTTCTTCTGTTGCGGGTAAGCCTAAGAAATTAGGTATAGAAATTTTAAAGAGATTTTTTACTAAACCAGTAGTATTAATAGCACTAGCAGTATTTATAATTATTTTACTGTTATCTATATTAGTTCCTGCTAATGGAATTTCTTACTATAAACCAAACGATCCAATTTCAGATAATGCATTTGTAAAGAATTTACCTCCTGCATCTTCTCCAATTGTTACTCAAGCATTAGAATCAAATGATCCCGTATTCAAATTTTATCAATTTGTCTTAAATAGGTCAGAAACCGATCCTGTATATAAGGCTAGAATGGCATTTTTCTTAAATGGCTTTCAAGCAAAGGTTAATGATATCGGGATCTATGTTGCAAAATATAATGCTTATGATTTAGTAGTTGCTAATTACATAAATGGAATTATTTCTAAGAATCAAATAACAGATGCTGAATATTTAAAAATATTTAATGAACAAAAAAACTTTTATTCTCATGTTTTATTAGGAACATCATCAATAGGCTACGATATTTGAACTACTGCATGATATGCAACATGAAGAGCAATTAAAATTGCTTTAATAGTTGTAATATTGGAAGCAATTATTGGAATATCAATAGGAGCATTCTTAGGATTCTATGCTGGAAAATTAATTGATACCATTATAATGCGTGTTATTGAAATATTCCTAGCACCTCCTACATTAATATGAATCTTATTATTTGTTTCAATTATTGGAGCAAATGAATGGTCATTAGTTTTAACGTTAACAATAGTTGGATGACCAGGATTTGTTGGAATAACTAGAATGTTCATTATTACAGTAAAAGATGAAGAATTCATCATTGCTGCAAAAGCAATTGGAGTTGGTACACCAAGAAGAATTTTCGTTCATGCATTACCAACTATAATTGGAAAAATTGCAAATAATATAGTAAGATCAGTACCAGGCGTTATTCTATGAATAGCATCACTAGCATTCCTTGGATTCTTTAAAGAAAAGAAGGATACTAACCTAGGACAAATGTTGATTGAAGCTTCGCAAGAAGTTGGCTCAAATATTTGGATTATTGCTTTGCCTACAATTATTTTATTGTTTTTATCATTATCATTGAATTTCATTGCTCTAGGTGTGCATGATGCACTTGACCCACGTGTAATGAACAAAGGAAAAGGTAAGTAAAATGGCAAAAATTATTAATAAACACGACAATTTAGACAAAAACAACTCAGATACTATAAAACATCAAAAAATTAATAAAAGTACTAGAGTTTTAGATTCAATTCGTGTTGAACCTCTAAAAAATAATCAAATATTAAAAGTTAGAAATTTGCATGTTAGTTTCCCTCAAGGTCATAAGAAAAGTATCCATATTGTTAGAGGTGTTGATTTAGATGTCCATAGAGGTGAAATAATTGGTCTCGTTGGAGAATCTGGTTCTGGTAAGTCAGTAACTTCAAAAACCTTAATTAATGTTAATGAATCAGGTATTATAAGTGCTGATCAAATTCAAATTGGCGAATATGAACTAACAAATATTAAAAAACAAAAATATTGACAATATATAAGGGGACAAAAAATTGGTTATATTCCACAAGACCCCTTAACTAGTTTAAATCCAACAAGAACAGTTGGAAAGCAATTATTAGATGCATTAAATAATAATGAAGATTGAAAAAACTCAACTTATACTGAAAAGAAAAATTATTTAATTGGTTTATTAAAAACATTTGGATTGCGTTCGGCTGAAAAAATATTTTATGCATACCCTCACACTCTATCAGGGGGTATGAAACAAAGAATTGTTATTACAATGGTTGTTGCTTTGAAGCCAGAATTAATAATCGCTGATGAACCAACAACGGCATTAGACCCAACAGTTCAAGCCAGCGTTTTGGCATTATTTGAAGATATTAGACAAAAAATGGGAATTTCAATAATTTTAATAAGTCACAATATATCTGTTATTGCAAAATTTTGTGATTACATTTATGTTATGTATGCTGGTAGAATTGTTGAAAAAGGAACCAAACAAGAAATCTTTACAAATCCTGCCCACCCATATACTTGAGCATTAATTTCAGCCATACCCGAATCAAAAGATGAAAAGCTATACACCATTCAGGGAACCCCTCCTGATATGGCAAATTTACCTCTTGGTGATCCATTTGTATACCGTAATGACTATGCATTAGAAATAGACTATTTAAAAGAACCTCCTCTAATTCCAATTACTGAAACCCATGCTGCCGCAACATGATTATTATCTCCTGAAGCACCAAAAATTGAAAAATCAAAAGAATTACAAAAACGTTTAGACTTATTTAAGAAGGCATTTAACAATGAATAATAAAAATTTTAAAGATAAAAAAGTTATCTTATCGATAGATAATTTAAAAAAATACTTCGTTAATCAAGGAATGATAAATAAGGCTGTTGATGGAGTTTCTTTTGATGTACATGAAGGAGAAATAGTTGGTTTAATTGGAGAATCAGGTTCAGGTAAAACAACCGTTGGTTCAACAATACTTAGACTATATGATGATTACAACGGATTTGTAAGATTAGAAGACAAAATAATTTCAGGTAAGCGCATTAGTGAATCTTTAAATCGTTTTTTAAGAAAAAATGTGCAAATGATTTTCCAAGATCCTCATGCTTCATTAAATAGTCAACAAAATATTTATTCAATTTTAAAAGAACCTTTATTAGTTAATGGAGTAATGAAGGACAAAATTAAAGATATTTTTAAAGATTGATTAAATGTTAAAAAGAATTTTAAATACACATTTCAAATCGAAGCAATGAGATTGGAATTACAAAATTATTTAGAAATTAACAAATTAGCTGAACCTTTATTTAAAAAGTGAACAGAAAACCTAAATAAGCTAGAATTCAATTTTGACATTTCTAGAGAAGATAACTTTAGCTTATTCTTTGGATATTTAGAAGAAAAGCAAAACGTTGAAAGCATTATTATTAATAATATGTATGCAAATGTTGATAGGTTAATTGAATTTTATTATGAAACACAACGTAAATATCGTAATAAAGAATTACCTGGACCACAAATTGCTTTAAATGAAAAAGAAGAAAAATTAAAATACATTCAATGTTTAAGCAAAACTTCAAAGATTGCTTATGAAGCTTCGCTTGAATTAAAAGAAGTTAAAAAACAAATATTTAATTTAAGACATAAAATTGAAAAAATATCTTCTGAAAGCAAAAATACATTTGCAAACTATATTCATGAATCGAAAAATGAAAAGAGTTTAATTGACATAGCAAGATTAATGTCATGTGATTTAGACTTTTATTCATATAATTTAAAAAGTGAATTGTTGATGAATAAAAGGGGTCAAGTATTGGCAAAAATAAAACCATTTAATAAATTCCTAGGATTTAATAATATAAAGAAGTTAATTAATGAACTAGATGAATATATTAATGAATTTTATAAAACAAAATTAGCCCCATTGCCATATTCAAAAACATTGAAATCTGACATTAAAAATGTTTTAGAAAAAGACTTTGATTTTAAATTCGATGAATATACTAAAATATCAGAATTAGAAAAACAAAAGTTAGATTCAGAATTAAAACAATTACTATCAAGAGAAGTTGAAATTAAAAAAGTAATAAAAGAAAATCCAAATCCTGAAATTACTTCAGAACAACTTCAAGAAGCAAAAAACGATCTAGAATTAGGAAAACAAGCATATCTTGAAGGTAGATCTAAATATTTAATTTCATATAAAGCTGAACTTGAAAGCTTGTATAAAAATCTTGAAGAACAAAAGAAATTCTATCTTGAATTGCGCAAGCAACAAGATTATTGCAATAACAAATATAAAGAATTGAAACAAAAATTCTTTGAATATGTTGAACAATTACGTCAAAAAGAAAAAGATAAGATTTTATTAAAAATAGCTGAATTAAAACAAAATCAAGACAAATCAAACAAAGATAAAAAGTCATTAGCAAAATTACAAAGCAAATTAAAATCAATTAAGGTTCAATACAATACAATATTAAAAATGTACCATTCAGATATTTCATTAAAAGAAGATACATTGAAATCATTTGATATTGAAAGAAAATATCTTGATAAAGATATAAATAATATTTATGTTTTATTAGGAATTGACCACAAGTGAGTAGAAACTAATTTAAAAACAGCTGATGCTATGGCTGGAGTTGACGCTAAACATATGAAGTGGGTATGACGTAAAAGATATTTTGATAGTAAAGTTTCATATCCTATTGCAAAATTATTAATTTCTTCAATATTGTATAAAACAATAATTTATAAAGCATTGGAAGATGTTGGACTATTAAAACAATTTGCTTATCGTTATCCTCATGAATTTAGTGGGGGTCAATTGCAAAGAATTGTTATTGCAAGAGCATTGATAGTTGAACCTCAAGTTATTGTGGCCGATGAACCTATTGCCTCACTAGATATATCAATTCAAGCGCAAGTTGTTAACTTGCTAAAAGAATTATGTATTAAAAAGAACATCGGTCTTATATTCATAGCACACGACCTTTCAATGATTGAATATGTTGCTGATGAAGTTCAAATAATGCACCTTGGAAAAATTGTTGAAAGTGGAAAAACCGAAGCAATATATGCAAATCCAATTCACCCATATACAATAAACTTATTTAAAGCTATTCCTAAAATTTCTAATGCTAATGAAAAATTCCAAAATGTTTCATTTGCTTTAGATTACTTAGATGAACAAAAATTCCCTAATGTTCCTGAAACATTTAAAGTTGGTGAAGATCACTATGTTTATGGAACTGCTAGTCAACTTAAACAATGAACACATAATGCAAAATTAGAGAAAGAACATAAAGAATTATAAAAATAATTTACCATTAAACTAAATAAAAAAATTAGACTCTTGACTAAAAACTTAAGAGTCTAATTTTTTTATTTCTTTATATAAGCTAATAATTTATTAATTTCGCTTGAAGTTAAATTTCTATATTGACCTATTTTTAAATTACTATCATCAATGCATCCAAAAGATTTTCTATGTAATTTTTTAACATAATTATTTACTAACAAAAATAAATTTTTAACGTGATGGTTTCTGCCTTCATAAAGTAGAACTTCATATTTAAAAGCACTTAATTTTGTAACTTTTTGTTTTGAAATTTTGCCATTTAATAATACGTGATTTGAATTTAATAAGTTTAGTTCACCATCAAGCATTTCACGTTCTAATGTAGCAATATAAATTCTAGGAATTTCAGAAGAAGGATGGCTTAATAAATTAGCAAATTCTCCATCGTTTGTGATTAATATAATTCCTGTTGTATTAAAGTCTAATCTTCCTATTGAAAATAAGCGTTCTTTTGCTTTGAGATATTCGTAAATTGTTTTTCTATTTTGAGGATCACTTGCTGTACAAATTGTATTCATGGGTTTATTTAAAACAATGTATTTAAAATTATTTGCAATATTATTATTTAATTTGATAATTCTATTGTTTATTTTAATTTCGTCATTTATAGTTACACATTCGCCTAATTTTGCAATTTTATTATTTATTAAAATCTTCCCCGATAAAATTAATTCTTCTGCTTTTCTTCTAGAACAAATGCCTTGCTCACTTAAAATTTTTTGAATTCGTTTTGCTTTTTCTTCCATTTTTAAAACCTTACTAAAAAGATATTTATATATAAATATATCTATTGTCGAGTGCTAAAATTATAACTTTTTTTTGAATTTATTTTTTAATTGGAGTTGGAGGAAAAATGGAATATTTACAAAATATAGACTTAAGCAAAATAAGATCGCTTTGTTTACAAAAAAAGCGGGAATTTATCTTTAATTATTGCCTTTTTGAGCAGTTAAAAGATAAACAATTAAAACAAAAAAAATCACAAACAATCAATGACGAAGAAGATGATGATATTTCAAAATTAGAAAAATTTATTAAATTATTAAATTCTGATAATCAAAAAATCTTTTTTAATAATTTTATTTATAACAATCCTGAATCTGAATGATATTTGGCATATTGATCAAAGAATGCATATTACAAAAAACTTAATTATATAGTTAATTTATTTATTGATTTTATTAACCAATAATGTTTCCTATTGTTATGCAGGCAATCAATGATCAAGAACCAACAAAATGGATTGAAAACATAAATGCTTATACAACCAAACAATTATATTTGGATAAATGAACAAATTCATTTCAAAAGGTTGATTTTAAATTACAAAGGTTAATTGAAAATAATAAATTTATAATTGAATTTAAATATTTGAATTACGACCAAGAAATAGATGATATAGATGTTAAATGTTCGATTAACAATCAATTATTGAAACTCCAAAAAGTCTTTGATTCAGATAAACATGAATATACATATACTCTTATTTCTGAATTTAATGAGGCAACAAAAACTAAAAAAGTTCAGGATATAAAAAATATTAATTTTGAAATTTTTTATAAAGTAAAAGGTTTATTGTATCAAGTAGAAAGATATACATATGAAATATATAGTAATAACAATTCTAATAAAGAATTAGTTTCAAATAAAAATGGCGTTGAATATGATTTATGAACTAAATTAAGAATTCTATCATTAGCAGATAGAGATATTCCGACAGATATGGAACATTATTCATTTGTTGAACAAGTTAAATACAAATTTATGCCAACTACTCTGGTGTTGGGAAAACATAATAAAAAATTATTTGATGTTGAAATATCAAAAAAAGAAATTTCTAACAAAAGTGGCATAATAAGCGATTTTTCTCAACTAATTCAAAGCGATATTAATGGTTTTAATGTATCTATTGATATTGATAAACAATTTATAAAAGGCAACTTGAATGCAAAAATTAATGATTTCAGTAAAAATAAGCATAAGCAAATTTCAATAGATTCTTATTCATATTATGACAAGGTTTCTAAGGCAATAATAGCAAATAATGATAGTATTGGCGCAGCTAAAGGCTTATTGTTGCCTCTTAATTATTCAGGAAATTATTCTTATTTACTAAATATTAATTTTGGTGAGGATTTGAAAGGTTTTAAAATTAGATACAATCAAGAAATTTCAAAACCATTTTTTAATGAAAAAAGTGGCTTAGTAAAATTAAATGTAAATAATATATTTAAACAACAAAATTTCATAAAATGAAGTGTCGTAAAAGCAGAGGATATAAGAAGTTTGATTAATAATTCCAATACTCTAGAAGAATTAATGAAGGGAGGGAAATAATGTTGATTGCAAAAAAGAAAAAAATAATTTTCGGTTTATCTCTTGGATTAGCTTGTGCCGTTGGCGCTGGCGCATTAGTTGGAGTTTATTTTTTAGCATCTTCTAATTCAAATAAAAAAGCTAATAATCTAGACAATAAAAAGGTTAACAAAAAAGATTCAACAAATAACAATAGTGACAACAAAAACATTAATTCTGGCAATGAAAACGAAAAGGATAACACTATTGATGGCCAAAACGAAGATAGTAAAATTGAAGATTCTAATGAAGATGGCCAAGCGAATGATGATGATAAAAAGCTTAAGCCAGAAGCAAAGCCAAGGCCGAATATAAAAACAGAGATTAATTGAAGAGAAATATTTCCTGATGTTAAAACTTCAGATTATTACAATCAATTAAATTTTAAAGATGGCCAAGGATTTATTGACAAAGAAATGATTACTTATATAATAAAGGATATTTTAAATAGGATGACTATTTTAGATGGCAATGTTTCTTATGCTTATAAACAAGAGAATGATCAAAATTTATATATTGCTTTTAAATGAGAAAATAAAAGTCAAAAATCTAATATAACTTATAAATTTAGTGTTAATAGAATATAATTAATTTTATGGAATCTGAAAAAATTATAATTAATGCTATTAAAAAACTAACTAAAACAAAATTTAATAAAGAAACTTTTATTCGTGATTTAAATATTGATAGTTTAGATTTGGTTATACTTGTTAGTGATTTAGAAGAGGCTTACAATATAAAAATAAGTGATGAAGAATTGCTTTCATTAAAGACCATAAATGATATTATTGAATTATTTGATAAAAAAACGAATAAATAAAGCAAAAATAATGTAAAATTATATTGCTTTATATGGGTATAGTTCAATCGGCAGAACAACGGGCTTCAACCCCGTGTGTTGTGAGTTCAAGTCTTACTACCCATGCCATATCAGAAATGATTAATAAAAGTAGCAAAATTTTGCTACTTTTTTTAATTTAAATTATAAAAAAAGTAGGGATTTTAAATCCCTACACGACATAAAAAAATGATATGGTGCCGTCAATTGGAATTGAACCAACGACCCCTTCCTTACCATGGAAGTGCTCTGCCCCTGAGCTATGACGGCATTAAAAGCAAAATTATTATAACAAAAAAACTAAGTTTTTGCTTATAATAATAAAAATAAGTTATTAATGTTATTAATAAATTAATTTAAATTTAATTATAGAGGCTATATGGAAAAGACAATGTACGATTCACTACTTTCAATTAAAAATCAATACGATGAATTAACTGAAAAATTAAACGACCCTAATATTTATAATGATATAAAAGCATATACAAGAATAGCAAAAGAAAAATCTGAGTTGCAAGAGATTTATGATGCTTTTAAAAAATACCAAAACATTGAAGAATTATATTTCTTGTCAAAAGAAATTTTAACAAACGAAAAAGATCAAGAATTAATTGAACTTGCAAAAAACGACATTGACAATCAAATGAATCAATTGCAAGAAATAGAAGAAGAAATTAAAGAACTTTTATTGCCACAAGATGAAAATGACAAACGTGATGTTATTATGGAAATAAGAGGTGCAGCTGGTGGTGATGAAGCAAATATCTTTTCTGGTGATTTATTTAAAATGTATCAAAAATATGCAGAAATCAAAGGTTTTAAAATTAAAATAATTGATTCTACATATGGCGCAGCTGGTGGATATAGTCAAATTGTTTTTAGTATAAAAGGCGAAAAGGTTTTTTCACAACTAAAATTTGAACGTGGAGTGCATAGAGTTCAACGTGTTCCTGCAACTGAAACTCAAGGACGTGTTCATACATCAACTGCAACTGTAACTGTATTGCCTGAAGTTGATGAAGATGTAAAAATTGAAATAAGACCAGAAGATATAGAAGTTAATGTATTTAGAAGTTCTGGAGCTGGCGGACAATCTGTTAATACAACGGACTCTGCAGTAAGAATTACTCATAAGCCGACAGGATTGGTTGTAACATCACAAGATGAAAGAAGCCAAATTCAAAATAAAGAAACAGCCTTAAAAGTTTTAAAAAGTAGATTGTATGAACTAGAATTAAGAAAACGTGAAGAAGCAGAAAGCGGACTTAGAAAACTAGCTGGAACAGGGGATAGATCAGAAAAAATTAGAACATATAACTATCCTCAAGATAGAATTACAGATCATAGAATTGGCTTTTCAACTTCATTAAAAGTAGTTATGGAAGGCGAATTAGACAAAATTATTGAAGCATTAATAGCTGATGAAAAGGCTAAAAAAATCATTGAAGCAGGAAATTAATGATAGATAAAGAAATTTTGCTACGTGAAAAAGAAAGATATAATTTACCCCTATATATTTCAAAAAAAGAATTAAGAATGCTAAAAAAAGATGTTCCTGTGCAAAAAATAATTGGATACCAGGAATTTCAAAATGTAATTTTAAATTTAAAATATAAAACCTTGATTCCTAGATATGAAACAGAAGAGGTTATTTTAGCTGCATATCCATTTATAAAAAAAGAATCTAAAGTATTGGATTTAGGGTGCGGTAGTGGCCTAATAGGACTTGCAATTAAAAAAAATAAAGACGCTAATGTTACATTAAGTGATATTAGCTGTTTTGCAATCAAACAAACAAAAATAAATGCCAAATTAAATAATTTAAATGTGAATGTAATTCGTTCAAATTGATTTAATAAAATAACATCCAAATTTGATGTAATAGTTTGCAATCCTCCTTATTTAAAAAAACAAAAGAAATTAATAAAATCATTAAAATGAGAACCCAAAAGAGCCTTGTTTGCTAGAGACAACGGTTTATTTGCTTATAAAAAGATTTTAAAATCTGCGTTTAATTATTTAAACGAAAAAGGAATAATTATTTTTGAGGTCGATCCTTATGTTTCTTTATGGTTTAAAAATAAATTTCCAAATGTTCTAATATTAAAAGATATAAATAACAAAGATAGAATAGCATTGCTAGCAAAAAATGATTTAAAAAATTAGCACTCTTTTTAATTTTGTGCTAAAATTAAAAATTATGGAAAATAAACGTGATTATTATGAAGTTTTAGGCATTTCTAAAAATGCCACAGAAAAAGAAATCAAGTCGGCATATAGAAAATTAGCAATGCAATATCACCCAGATAGAAACAAAGCGCCAGATGCTGAAGAAAAATTTAAAGAAGTTTCAGAAGCATACGAAGTTTTATCTGATCCTGAAAAAAGACAAAAATATGATAAATTTGGACATTCTGCATTCGATCAAAGTTCATTTGGATATTCAGAAGATGTATTTAATAGTTTCTTTAATTCTTTTAGAGATATGTTTTCTGGCGGAGGTTTTGAAGTTGATTCGAGTGATGATTCAGATGATCCTTTTTCAAGTATTTTTGGTTTTGGTTCAAATAGATCTAGAAAAACAAAGGGCAGTGATTTGCAAATGAAATTGTCAATTGATTTTTTAGATTCAATATTTGGAAAAAATGTAACTGTTCAATTAAAGAAATATAGCGAATGTTCACATTGCCACGGTTCAAAAGCAGAAAGTGATAGCGATATTGTTTCATGTCCTCAATGCCATGGTAGTGGTCAAGTTCAACAAAAAATGGGTTTTTTTTCAACAATTAGTCCTTGCAACAAGTGTGGAGGCTTGGGTAAAAGCATTAAAAACACATGCCATGTATGTAGTGGAAAAGGATATGAATATAAATTAGTGAATGAAAATATTCAAATTCCTGGTGGAATTAACAATGGCGAATATATTAAATTGTCAGGATACGGTAATCCTTCTCGTAATGGAGGATCTAATGGAGATTTATATATTATCATTGCTATTAGACCACATAAATTTTATGAAAGATTTAACAATGATATTGTAGTTTCAATGCCTATTTCTGTTTTTTCAATTATTAATGAAGATACTATTAATGTTCCTACACCATACGGAAATAAAGATATAAAATTAAATAAAAATATGAAATTAGATGGAGTAATCACTTTAGATGGCTATGGTTTTCCTATTAAAAATAGTTCTAAAAAAGGCAACTTCATTATTACATTAAAGCCTTATATTCCTTCGCTTAATAAAGAAGATAATAAAAAAGTTCAAGAAATATTTAGCAACTATAAAGATGTAGAATTTCAAAAATGAATAAAAGATTTTAATAATTAAATTTAAAAATGCATTTATTAATTTTAATAATTAATTGCATTTTTTTATATTCGAATAATTAAACTTGTATAATATTTAATTATTTTTTACTTTTAATAAAATAATAAATCAATGTATCAAAAGAATATATTCAAAATAACAAAATTATTTTTCAAAGGAGCAATATGGAAAATAAAATCATGATTGTTGAATCACCAAATAAGGTTAATACTATTCAAAAGATAGTAGGTGATAACATAAAAGTATTGTCAAGTATTGGCCATATTTTAAAAATGTCAACATCAGGCATGGGGGGATTAGGTATAGATTTTGAAAATTGAGAACCTAAGATGGTAATTGATCCAAGCAAGAGAAAAATCGTATCTGAATTAAAAGAAGCAGCTAAAAATGCTAGTGAAGTTTTAATTGCAACTGACCCCGATCGCGAAGGCGAAGCAATTGCAAATAATTTAGTAAATACTCTAAAAGTGCAAGATAAATATAAAAGAGTGAAATACAATGAAATCACTCCAGATGCCATTAATTACGCAATTGAAAACCCTTTAACAATTGATGAAAATTTAGTAAATGCTCAAAAAGCAAGAAGAATATTAGATAGGATTATTGGTTTTAAATTGTCTCAATTAATCCAAAGAAAAGTAAAAAATACTCCAACTAATCCATCTGCTGGTCGTGTTCAATCCATTGCTTTAAAACTAGTAGTTGATAAAGAAGAAGAAATTGCAAAATTTATTCCAACATTATATTCAAAAATTGAAGCACATACTGCTGATGAACAAATTGCAAATTTCGTATATAGAGATAATTTAGATTTTGTTGGTGAAAATGAATGAATTAAAAGAGAAAAAGTAGATGATATATTAGCTCAATTAAATCAAAATAAATCATTGCAGGTAATAGACTTTAAAAAATCTATTCGTTCAGAGGCAATGGTTGTTCCATTTAAACAATCTATTTTATATAAAGAATCTCAATATAGTTCAGCAACCGTTCAAATTGCTGCACAAAAATTATTTGAAATGGGGTTAATTTCTTATCCTAGAACAGATTCAACTAGACTTTCATCAATATTTATTGAAAAATCCCAAAAATTTATTGCCCAAAAATATGGAAAAGAATATATAGCAACTGAAATAAAAGGATTTTCTGGTGATCAAGATGCCCATGAAGCAATAAGACCAACAGACATTTCACTGCTTGCATCCGAAGCTCAACAAAAATTTAATTTAAGTAATGAAGAAACAAATGTATATAAATTAATCTATGAAAAGACATTAATGGCTTTGATGGCTACACCAAAACGAGAAATATATCAATACGAATTAAAAAATGGCCAATATAATTTCAAAATGTCATATAGTAAAATAATTTTCGATGGATATTATAAAATTTTTGATAAAAAACAAGAATCTATTATTCCTAATTATAAAGTTGGAGATATTATTAAAGTTTTGGAATTTGCTAAATTAGACAAAGAAACTAAAGCTCCTGCAAGATATAATGATGGTTCGTTAATTAAGATGCTTGATGATATAAAAGTAGGAAGACCATCAACATTTGCAACGACAGTTAAAATTATAAAAGATAGATTATTTGTTGAAACTAAAAATAATAGTTTATATCCGACAGATTATGGAAAACTAGTTTTAGAAAAATTAATTCAAGGTTTTCCAACTACAATTAATGAAGAATATACAGCTAGCGTTGAAAACCAACTTGATTTAATTTCAGAAGGATTATCAGATTATAAAATTTTATTGAAAGATTTTTGAAATAATTTTAATTTGCAACTTCAAAATGTAAATGAAACAATGGAAACAGATTTCTTACCTCAAGAAATTTTGCAAGAAAAATGTCCTCTTTGTGGACATGACTTGCTTTATAGATATACTAAATCAAAACACCAAAAATTTATTGGATGTTCTAACTTTCCAAGTTGTAGATATATAAGAAATATTGACAATGAAAAACCTAAATTTTTTAAAAGAAGATATAATAAATAATTTTTATTTTTGCCTAAATAATAACAAATGTATTAAAAAATTAGATTTTATTACAAATCTAATTTTTTATTACTTATTTTTATTGAGAAATTATTTTAATTTATTCCTGAAATTCTATCAATCATTATAGGATTATAATCTTTGCCCTTGTCATTGCTTCCTAATTTGTAATTTAGACCAAAGGACTTTTTGCTATTTTCTCCTTTTGTTGAAGTAACAAGATTATATTCATAGTTTGAGTTTATTGATACAATACCTACAAATCTATATCTATTTTCTGAACCACCCCATTTTGCTAATCCTTTGAAATTTGATTGTCATCTAGGTGGAACATTTTCGGTACTGAATTCAAATGTAACATCTTTATAGCCCTTAAAAATATTGTCTTTATTTCTTAAGTCTAAATCGTTAGTATTTGGTCTTCCAGTTTTTGAATAAATATCAATATTGTAAATAGTCAATGTTTTATGGATGGTTCCGTAGTATTTGTCAGTGGTATCATTAAATAAATTTTGACATTTTTTAATAAAACTAGTTTTAGCAGTTCAGAAAAATGCATCTTCTAATTTACCATCAAATTCACTTCACTTTACAGCTGGCCCTTCTAATTCATTTTGTTTGTTAATTTCTTCTTTATATTTTGGATCATCATAATTTGGCTTACCATCTTTTAATGGCACATTGCCTTTATTATAGAATGTTTTATAAAAATCTTCAAAATGATTCATATTCATTTTTTCTAGTTGGTCAAAAACTTTATCTTGACTTAAATTATTTTTTAAATTATCAAAAAATTCATTTTTTTCTGCTTCATTCTTTTGTACTGATGTATCGTCGAAAAATTTATATGCTGCTTTTAAAATTCCTTCATATCTTGAAATTAAAGCTTTGTCTAAAAAATCTATTTTATTAATGCATTCATCTTTTACCTTACCGTCAGCATTTTTATTAATTAATTCTTTTTCTATTTCGTTTATGAAACCTTGAAAAATTGCCTTTATACCTTTGTTTTCGTTTGAAAGACTACCTAAGGTACCAATGTTTTCAGTATCTAAAAATTTTAACTTTTCTTCAATTTTTTGTTTTTGAGAATTAAATTCTTTAATATTATTTATTATTTTTTCAAACTCGTTTTTAGCAGGTTCTAATTGTTTTTTAGCATTTTCTATATCTTGAATTTTTGAATTTTTATTTATACTTGAAACACCATTTATTTTATTTTCGATTTTTGTTCTAATTGAATTATATTTTTGATCCTTCAAATCTTTATTAATAAAATTTTGCAACTCATTTTTTATTTGTTCAAATTGAGAAAATTCGCTGCGTCTTTTTTCTTCAATTTTATTTTCTATTATTTTTTTAATATCATTTATTTTTTGGGTTGCTTGTTTAATTTCTTCATCTTTTGAAGCATCCGATATATTTTTATAATTTTTTAAAATTGTATCTGCTTCTGCAGTTCCTGCTTTTTTAGCATCATCAGTTTTTATCAAATTTTCCAATTGCTTTTTGCTGTTGTAAAAATTAGTAATATCTTCTTTTGTATTCTTTGCATTATTTAATTCATTTTGCAATGATTGATTTGCATCTTGAATTTCTTTTTTATTATTAGAAATAGTTATTGAATCTTTAGCAATTTTTTTATTTTTTAAGTTTTTAATTATATTTTGACAATTGTTTTTAGTTTGAGGAACATTTTCAATTTGTTTTATGAATTCATCAATATTTTTTCTTGTTTGTTCTAATTTTTCATATTCTTTATTTTTTGATTCTATGAATTTATTAAGTTTTTCTTGAATTTCATTAGTTTTATTTGTTAATGAATTATTTGAATCAGTCAATGATTTTGTATTTTTTGAAGTAATTGCCTTATTTGCGTTATTTATTTCTTCTTCAAGTTGTTTTTTATAATTTTGAATGTCAGTATCTGAATCAGTTAATTTTTTTAATAATTCGTTTGCAGTAGATAAACTGGTGGTTAATTTATTATTAGCTTCGCTATTTGCTCTATCCGCTTGTTTTTTTGCCTCTTTTGCTTCTTTCAATGCATTAGTTAATTCATTATTTGCAGCAATAATATTTGCTTTGTTTGAAATGTTGTTAATTTTTTCTTGCGTTTTTAAAGAATTTTGTAATTTTTTAACAAGATCAGCATAATTTGGATTGGTTTTAACATCATTTGTTAAGAAATCATTAATATCTTTTCTTGTTTTTTCTAATTCTTTAAACTTTGTTCCTTTTTCTTTATTAAACTTGTCAAGACTTGATTTAAATTCATCAATTTTGTTTGCTAATTGTTCTTTTGCTTCAATAATTGCTTTAGTATCTTTTTTAAGTGCTTCGTTAGCTTTAGCAATTTCTTTTTCCAAATTAGTTTTTAGCGTAGTTATTGTAGGATCACTATCGATTAATTCTTTTTTAAGTTTATCTGCCTCTTGAATTTTTGCATTTAGTTGTTCTTTGGCATCAATTTTTGCTTTTTCTTGTTCGGCGTTAGTTTTAGCCTTGATTAATGCAGCTGTTGCATCTTTAATTTCTTGAATTTCTTTAGTTGCTAAAGATTCTTCTTTTATTGCACTAAAACTATTTATTTTATTTTGTGCTTCTTTTTTAAGAGAATCGTATTTTTGATTATTTAATTCATCTTTAACGTATTTTTCTAATTCTTTTTTTGCTTTATCAAATTTTGAATATTCTTGTTTTTTGGCATCTGAGATTTCTTTATTTATATTATCTATTAATTTTTTAATTTCTTTTGATTTTTCTTGAATTTCATTCTTACTTAATGTTTCTAAATCCAAGACTTCATATTCTTGTTTTTTATTATTAAATTCAGTTTTTTTAGATTCTTCTAATTGTTTAAAATTATTATTAGAATTTATCAAAGAATCTAATTCGGTTTTTTGCTTATTGAAATTGTCTATAGATTCTGTTCTTTGAGTTGAATAATCATCCAATAAACCTAATAGTTTTCTTTGTGTTTGATCGATACTATCTTTTGCTGCTAAAATATCAGAAATTTTTGAATTTTTGTCTATTTTGTGTTTTTTAATTTCTTCATCAATTTGTTGTTTTATTGATTTTATTTCATCATCAATATTAATAAATTTATCTAAACCTTTGGTTTTGATTATGTTTTCTAGATGTTGTTTTTCTGTTTTAAATTCTTCAAATTTAGCTTTTTTATCTTCATTAATTTTTTGAATATCTTTAGTTAATTTATCTATTGCTGCTTGCAATTTTTGGTTAACTTGTTCAACATTTTCAATAGTTGAACTATTATTTATAGCCTTGGCATCAGTAGAAGCTTGCTCTTCAGAAGATAAATCAATATTTTCGGATTTTGCATCATCTTTAAGATTTGCAGTTTTTAATAAATCTTCAAGTTTTGACTTCAATTCATAAAACTTATCTAATATTGCCTTGTTGCTTCCAATTTCTGTAAATTTTGCATTTAAATTATTTGTAGCTTCTTTAATTTTTGAAATTTTGTCGTTTGATGTAATGTTTTTATATTCATCAATAACATTATTAAATTTATCTTGAATAATTTTATATTTTGAATCTTTAATATCGTTTGTAATGTAGTTTTGCAATGCTGTTTTAGATGATTCAAATGCATTAAACAACTCTTGTTTTTTGTTTTTAATTTCTTCTTTTAATTTTGTTTTTGCATTTGTTAAATCATCAGCAGCTTTTTTAATTTCAGTGATTTCGCGTGAATCATCTATGCCACTAAATTTTGCTAATTCTTGTTTAGCATCGTTTAAATTTGAAACTAATTCATCACTTTTAGCATCTTCATCATTTTTAATAAATTCTTCTAATTCTTTTTTAGCTAAAACGAATTTATCAATTTCTTGTTTTTTATCATTTGCTTCTTGTAAAGAATTTTTTAGCAAAGTGTTTGTGTCTTCAATATCTGATTTATTTGAAGATTTAGAAACTGAATCTTTTTCATTTTTAGTCGTGGTTAATTTTGAAATTATGTTTGAATAAGTTGAATTATCCTTATTTGAATTAATAAATTCATCAATGTCTTTTCTTGTTTGTTCTAATTCATTGAATTTAGCATCTTTATCTTTATTAAATGTTTCTAATTTTTTAGTAATTTCAGTAACTTTGGCATCTAGAGATTCTTTTGCTGATTGCATAGATGTTGGATTATTTAAATTAAGCTCTTAACTTGCACTTTGAATTTCTTGACTTAATTCAGTTTTTGCTTTTTGAATGTCTTTGTCTGAATCAACTAATTTATTTAATAATTGGTTTGCATTAGTAATTGAATCGTTTAATTGTTCTTTAATTGATTTATTTGCTTCATCAACTTGATCTTTTGCTGCTTTTGCCTTTTCAAGGGCTTGTTTTAATGCTTCATTTGCGGCAATAATTTCTGATTTATTTGAAGATTCAGAAACTGATTCTTTTGCTTTTTTGGCATCAGTTAATTTTGCAATTAAATCTGCATAATTTGGATTGGTTTTATTTGCATTAATGAATGCTTCAATTTGACTTCTTGTTTGTTCTAATTCTTTAAATTTAGCATCTTTATCTTTATTGAATGTTTCTAATTTTTCAGTAATTTCAGTAATTTCAGTAATTTTGGCATCCAATGAAGATTTAGCAGATTGCATTGAAGCATTGTCATTTTGGTTAACTGCTTTATTTGCATTGGTAATTTCTACATTTAATGATTCTTTAGCTTTTACAATTTCACTATCATTATCATTTAATTGAGGCAATAATGTGTTTGCTTTGTCAATTAATGCATTCAATTGTTCTTTAATTGATTTATTTGCATCATCAACTTGATCTTTTGCTTTATTAGCATTTGCTAATGCTTGTATTAATGCTTCATTTGCGGCAATAATGTCTGATTTATTTGAAGATTTAGTTAATGATTTTTTAGCATCTTTTGCATTGGCTAAATCTTTAACTAAAGTTGCATAATTTGGATTATTTTTAACGTCATCTGTTAAGAAATTGTCAATATTCTTTCTTGTTTGTTCTAATTCAGTGAATTTTGCTTCTTTTTCTTGATTGAATTTATTTAAGATTTCTTTATATTCAATAAGTTTAGTATTCAATAAATCATTGGCTGATTCCATTGATTTTTTGTCTTTTGAAAGTGATTGTTCTGCTTTAGAAATTTCATTATCTAAATTAGATTTTAAATCAACTATTTTTTGGTCATTGTCAATTAATAACTTTTTAAATTCCTTGGCATTATTTATAGTTTTTTTAAGAGTATCTTTTATATTTTGACCATCTAATTTTTCTTTTTCAAGGCCCGCTTCTTCTTTTGCTTTTATTAAAGCATTTTGCCCAGCTTTTATTTCTTTAATTGTTGAATCTTTATTTATTCCATTTAAACTATTTATTTTATCTAAAGCCTTTTGTTTTATTGATTGATATTTTGCTTCATTTAATTTTGTGTTAATAAAATCTTGTAGGTCTTTTTTAATATTTTCAAATCTTTGAAGTTCTTGTTGTTTGGTATTTAAAATTTTTGATGTTAAAGAAGAAGTTGCTTTTTCAACTTCAATAATTTTGTTTTTTATATCTTCAATTGATGATGTTTCACTTATATTTGTTTCAACAAAAACTTTTTGTTCTTTTATAAAATCTACTTCTTTTGCATCTTCTAAATCAAGCACTCTTTTTAATTCTTGTTTTTTATCATTAAAAATTTTTGATTGTAATTCTCTTTCGGTATTTATTTTATTTAGAAGTTCTTTTTTTGCATTTTCAAGATTTTCATTTGTCTTTTTAATTTTATCTATTGGCAAAGAATCATCTATTTGATTTTCTTTTAATGCTTTTTCAGCATCAGAAGAATCGACGTTTTGACCGTCATTTGAATTAATTAAGTTTTGTAAATCTAATCTTAATTTATTTAATTTATTTAATTCTTCTAATCTTTGTTTTTCATCTTTTTGTAGATTTTCTAATATCTTTTTACTAAAAATTTCTATTTCATCAATAAGTTTTGATGTGTTTGAATGATTCTTAAATGCTGTACTATTAAATTTCTTATCTTGTCTTGCAATAAAATCATTTGCTTTCTTTTGACCTTCATTATCTAGTTTCTTAATCAGATTAAGTAATCTAGATTTTGCTTCGTTATACTTTTTCGTAGAGTTATTGCTTATCGAGCACATAGCAGCAATAGCTGACGTTGATGCAGCAGCACAAACGATTGAAATTGACATTAAAATGTTTATTCTTTTTTTCATAATTTTTTTGTTATCCTTTCAAAAGCAATATTTAAATAAAATAATAATAGTTATTATTATATTTAATAGCTAAAGAATTTGAAATTAAACAATTAATTAGTATTTTTTTTAAACCATTAGGTGCATTAAATCTGTTTTACAAAAAATATTTTAAATTAAGGAATATTTTTTATTCTTTTAACTACTAAAAATATGTAAAAAAAATATTGTTTTAATTTTTGAAATAGAAAAAATACTTATAAAAGCATAATAAAATACTGGTTTTAATATAAAATTAAGTAAATATGAAAAATCATTCTAATACTAGACGAAAACATAGATCATTAGCACCTTTATACTATACCCTTGGTTCAATTGCAGCCACTGCTACAATTGCGGGGGCTATATATTTTGTAGTAAAAAACGGTAGAAGAGTTAGCAAGCAATATTGAAGTGTAGAAAAATTTTTGCAAATTAGTAAGCATATAAAAATTGATCCGGTTGTAATTAATTCGGCTTCTGCAAAAGAAATTTATGAAAATTTTATTCATGAAAAACAAAAAGTAGATGCGTTAAGAAATAAATATATTAATCAACATCCTGAGTTAAAACCATATTTGAATAATAATACTAGAACGGCATTTGATTTTAAATTTAAAGGTCCTAAAAAGTCCGAAATTAAAAAATTACTAAAAGATATGCCTGAAGAATTTGACGCTCATAATTTCATTTTTTCAAAAATTTTAACAAATCAAACATTTATTGAAATGAAATTCTTAAAAATGAGATTTTCTAATTTGGAATTAGACACATCTGATAAAGAATCAAATAAACTAAAAGTAACATATGAAGTTTATTTGAATTATGAATATGCAACAGGAAGCTTCGAACTTAATAATGTTAAGGGCACTTCGCAATCTAAATATTATTTTAAAGGAAATCAAACTGTCAAGGTAATTAGTGATTCCGAAAAGTGAAATTTGAATTCTAAATTTGCTAATAATCTAGAAAAAAATTTAGATGATGCTAGAAAGATTATAAGAATTGGAATAGTTAAGAATGATCCAAAGTGAACACAATCAAATGAATTTAGAGAAATGATTTTCAAATGATTTAAAAGAATTTTTATAGAAAATGATGCCTTTCCTGACACATATAGCAAAGATGAATATGAAATAATGCCTTTTAATGAAACAAGTAGTGGTGATGATTATGTTAGATGAAATCCCAAGAAAGGATTAAATAATCTAACAATTAAGTTTTTTTATATAAATAAAAAAAATCCAGATATTAAATCACCTGGCAGGATTAAGATTTTTACTATATTAAAGGTTTAGAATGGAAATAATTAATAAAGATCTAGAGGCAAAATTAAAAACGTTTCAAAAGCAAAATTATAAATTGCAAAAAATTTGTCAATATTTTAAAATAGATTTAAAAAAACTTAATTTAAATGAATTTTTAGATTTTAATAAAAATAGTGAAAACTCTTTATTGGATTTAAAACAAATCAAACAAATTATTGCAGAAAATCAAGGTGATTCCAATATCCTTGAAGTTTTAAACATGATTAAAGAACTAGAAAGTATTAATAAAGAAAAAACTGCAAATGATGAAACTTATAAACAAGATCAAATTTTAAAAAAAATTTTAAGTAATTTAGAGTAATGTGTCTATAATTAGTATTAATTTAGTTATATGCATCAACACGTTACAAACGTAAAGAAAGTAGGTTATTATGAAAACACAAAAGCGTATTAATATTGCAATTGATGGACCTGCTGGTGTTGGCAAAACTATTATGTCAACAATGCTAGCAAAAACATTAGGTTATAAATTTTTAAGTAGTGGTAGTTTTTATAGAGTTGTTGCCTACAATGCATTACAAAACAGACTAGATTTAAATGATGAAGAATCTATTAATAATGCATGAAATATAGAAGATATTCATGTAACAGAAGACGATAAAATTATTTTTAGAGGTCAAGATGTTAGTAAATTAATTAGGCAAGAAGAAGTTTCTATGGCCGCATCAGCAATAGCAAAATTTCCTTTAATAAGGGCAAAGGTTAATAAGTTTATTCAATCATTTGGTCTTAAATATAAAGGAATTATTGTTGATGGTAGAGATGCAACATATAGAATCTTACCGCAGGCAGAGGTTAAATTCTTTTTGTGAGCTACACCTGAAGTAAGAGCAAATAGAAGAGTTGCCCAAGATTTAGAATTGGGTTTGAAGGCCGACTATAATGAGGTATTAGAAGATATAAAAATAAGAGATTATAATGACACAAATAGAAAATTTGATCCATTAAAAATTAGCGAAGGCAGCATTGTTATTGACACTTCAAATATGAGCATTCAAGAAAATTTTGACGTAATGTATAAAGAAGTGCGAAAGAGGCTTGATGATGAGAAATAATATTGTTGCAATAATTGGAAAACCTAATGTCGGTAAAAGTACCTTATTTAATAAAATCATAAACAAAAGAAAATCCATTGTTTATGATACGCCTGGGGTTACTAGAGATAGATTATATGAGGATGCAATTTGAACTGGCAAAAAATTTAGAGTAGTTGATACCGGCGGTATAACTATTGAAGATGAAGATTTTAAAAAGCAAATTAAACTTCAAGCACAAATAGCTATTGATGAAGCAAATGTAATTGTTTTTTTAATAGACGGCAAAGAACCGCTTACAGTTGAAGATTATTATGTTGCTAGTTTATTAAGAAAAAGCAATAAACATGTTTTGTTGGCAGTAAATAAACTTGAGGGTTCAAACGTTAATTATTATGATAATTCAATTTACTCGCTTGGATTTGATGAAATATTTCCAATTTCAGCAATTCATGGTGATGGAATTGGAAACTTATTAGATAAAATCATAGAAAAATTAAATTTTAGTGATAAAGATGTTGAACCATATTTTAAATTGGCATTGCTTGGAAAAACCAATGTTGGTAAAAGTACATTGCTTAATACCTTGGCTAATGAAGAACGTTCAATTGTTAGCAATGTCGAAGGGACAACGCGAGACTCTGTTTCATCTCTAATAAAAATTAATGGAGAAATCTTTGAGGTCGTAGATACAGCAGGTATTAAAAGAAAAAGCAAACTAACTGAAAGTGTTGAACATTATGCTTTAATGAGAGCAAATCAATCAATTGAAGATGCAAATTTATGTCTATTGGTTTTAGATGCAACTGAAGAAGTTAGCCATTTTTCTCAAAATGTAATTGGCATAGCCTATGAATTAAAAAAACCATTAATTTTGATTGTAAATAAATGAGATTTAATTGAAAAAGATACAAACACAATGGAAAAATATAAAAAAGACTTGTTGAAAAAAGTTAAATTTGTCGATTGATCTCCTATTGTGTTTATATCAGCCAAAGATAATTTAAGAATAAATAAACTAAGAGATACTATTATTCAAGTCAAGAATAATATTTCAAGAAAAATCAATACAAATCAATTAAATGACTTAATGGCTACTGCTCAAATGATAAGACCTGCTTCATCAATAAACGGCAGAAGATTATCCATTACATTTGCAAGACAAATAGATGGATCAATTCCAACATTTTTATTGTTTGTAAACAATGTTGATTTAGCCCATTTCACTTATCTAAGATATATTGAAAATCAAATAAGAGAAAATTATGATTTCTCTGGAACTCCTATAGAGCTAGTTTTAAGAAACAAAAACAAAAAAGAAGAGAGAGAATAACAATGGCAAATATTTCGATTTTTGGAAGTGGCGCTATGGGTACTGCTGTAGCAACTATCTTAGCAAATAATGGTCACAATATTACAATATATGGCATAGATGATAGTGAGTTAAATGATCTAAAAAATGGTAGAAATACAAAATATTTTGAAGATGTAAATTTACCAAAATTTAAAGTTACAAAAGATAAAAAACAAGCGGTTGAAGATTGCAATTATATAGTTTTTGCAATTCCAACTAAGGCTTTGCCTGAAACATATAAAGAAGTAATTAAATTAATAAAAAAACAAGTCATAATAATAAATGTATCTAAAGGCTTTTGAAAAGATTCAAATAAATCTTGCCACGAAATAATGACACAAGAAGCCCAAGCTAATTCATTAATAAGTGGTGTAGTTTCATTAATTGGGCCATCTTTTGCAATAGATATAATTAATAAAAATATAACTGCCGTTGACGCTGTTTCTAATAATGATAAACTAAATAAAAAAGTCCAAAAATTATTTTCAAATGATTATTTTAGAGTTTATACGCAAAAAGATGTGGCAGGAGCAGAATCAGGATCAATTTTTAAGAATATTTTAGCGATTGCTAGCGGTATGGTTGCTGGGCTTGGATATAGCACAAATAGCCAAATAGCCTTAATCACTCGAGGATTTAATGAACTAAAAAAATTTGTTTTAGCTCGCGGGGGTAAGCTGAAAACGATTTTTGGATTGTCGTGCCTAGGTGATTTAATGCTAACAGCATTGTCAGATAAATCACGAAATTACACATTTGGAAAAAATTTTTATAACAAAAATTTTGATAGTTCAAAATTAACAGTGGAAGGTCTTAGATCAATAGAAATTGTTTACGATGAATATATAAAAACAAAACTATTGGATTTGCCTATTGTAAATTCGCTTTATCAAATAATATACAAGAATGCTAACCCTAATGAAATAATAATTAATTTAATGAAAAGAAAGCTAAAATCAGAATAACATAAGGAATAACAATGAACAAGAAAGAACTACTAAGTAAAACTAGCAATGAAACAGGATTTGCGCATGTATTAATCGAATCTGCATTTGATGAAATAATCAGAATAATTGCCGATGAAATAGAAGCGGGAAGAGAAGTTACAATTTCAGGATTTGGAAACTTTTCTTCTAAATTTATAAAAGGCAAGGAAAAGGTTCACAACATTACTAAAGAAATTATTAATGTTCCAGATAGATTGGATCCACGTTTCAAATTCTCAACAGCTTTTAAAAAACGTATTAACAGCAAAAATTAATTTAAACGCCGTTTATGGCGTTTTTATTTTAAAATATTCAAAAAATCTAATATTCCAGGAAAAATAAGTTTTATTTCTTTACCTAATGATTTTATTGTTTCAAAACAAATGTAAGATTTTTTTTCTTCAAATAGACACTTTTTAAAATTGGCATGTTCAATCAATAAAAACTCATTTTGAAATTTGAAATAAATTATTCAAAACGCAATTCCTTGATGCAATAAAACCTTATTTAAATATTCAATTTGATGCTTTTTAATATTCTTTTTTTCAAAATTTTTTTGCTCTGTGCTTTTAACTTCAAATGCTAAAAATCTTCCTTTTCAAACTCCATAATAATCTACAGTGCTTTTTGAAAATATGCTTGCATTGTTTAAAAGTAATTTATTTTTTTCTAATTTGACATTTTTAAATTTAATATCCAAATTTTTTTTATGAATTAAAGCAATTCCATTTTTAAAATAATACTCGTTAGTGTTATTTATTATAGATTCTAAAAGCATACCTCTATTATTTTTATTTTTCATATAAAAATTACAACATATTTATGCCTTGATTTTTTCGCTACTTTTTCGCTTTTAAGTCTTTATATGTTTCGAAGTTTTTGAAATAATTATCTTTCGATTGTAACAAAAATTCAACCAAATCAAATGCCATAATCATTGCCAAAACATCGTTATGGCAATATCTTTTTAATTGAGCTTCTTTGACCGTCCATTCATTATCTCTTATTTTATTTAAAGCTCTTAGCGTTGCTATTTGCAATGCCATTCCGCCATTTTTTACATCAAGTTCGTGATATGGAAAAATTTTATGTTTTAAATTTAATTTATTTTCATTTACAAAAAGTTCAATCTTTTTTATTGAGTAGACATTTTTTATTTGCCCTAAATTTACAATAGAGCTTTTTATATTGCAATCCATTGCTGGTCTAAAAAATAAATTTTCTATATCAATAATCTTGCTTAATATAAATTGAGATTTTTCTTTTAAAATGTCAAATTCATTTTGGCTAATAAATCCATCATCTTTGTAATATTCCAAAATTTCTTGCATTTCTTTTATTCTAGATGCTTCATATGTGTCGTTATATACGACATAATAATCTGCTTTCTCATCATATAAATCATTTATTACTTTCATAATTGTTTTATAGTTGTAATTCAAAGGGTCATAAACAAAATCATTTGAACTATATATTTCACCGTTTTGAGTTTTTATTATAGATGTTTGACTTATTAATTGATTTCATGCTAGAGAGTGGTTAATCATTGGAACGGGCAAGGTAACGCCTTCGAAATCAAACCAAACTATTTTTTTATTAAATTCTTTTAATAATAAATATTCAGGGCATTTATCCTTGTTTAAAATATCGGGTGAAATTGAAATTAAATTGTTTTCATAAAAATCTAAAATTTCTTTGGTTTCAATTGAATTATCAGTTAATGATTTAACTTTTAATTTTAATAATTCTTTTTGACAAATAATGTATTTAGGCATAACCTTTTTAACTAATAATTGGTAGAATTTGATTCCTAAATTTAGATTATCTTTTAAGTCATCCGTTGTTATATTGCAATCTAGTTCGTGTATTTCTTTATTACTATCAAAGTTTTCAATAATTCTTAAGAAATCAGAAAAATTGCAAGTGTATTTACTTATCTTGCTTTTTGCATCGTTATCTTCAATTTCAAAACGACTATTAAAATTAAATGCATTATTTAAATTTAATTCATTTTTTACATAATCAATTATTTTGTCATTTTTTATTTTTTTATTATCTTCATTAAGCAGAGGATTTTTATCAAAAATAGCATTAATTTCAGTTTCTGATAAATTCTTTTTTTTGTCAAATGTTGCTTTTGATTTTTTATAATTGCAATATTCAGTCTTTATTATTTTTAATTCATTTTTTTTCGAGTTTTTTCTTGTTTGATATCAAGGTTTCACAATGAAAATTTTATTGATTTCGATCCCTGAGGATGAAAGAACGGAATAATCATAATATGCTCTTAATAAATCCTTTAGGCTAGTTTTTGATGAATATACAATATTTCCCATTTCCTTAGTTTTGCTATCGTAGTAAAAAGGCTTTGATATGCATTTTTTATATTCAAAAACAGGATTTATTATTAGTGAATTTTGCATTAAAAATTCTTTTGTTTGTAAAATTTTTTCATCGGTTGAAGATTTTTTAGAAATTATATTTACTTTATTTAGAGAATTTTTTGTCAAAAAATTTGTAATTTGTTCCTCAACAAGGGCTATTCCACTATCAACCATTTGAAACTGAAAAAAATTATCCAATATTTTGTTAAATAATTCTAGTTTACCATCTGAATTAATTTTTTCAAACAGTGTTGAATCATTTTGCATATCATGTTGAATTTTTTTTATTTTTTCAATAGTGTTTAGCTTTAAAAAGTCTTTGTTTTTAGTAGAGTTATTTTCAAAGTATTGATTAAAATTTTTGTCAAGAATGTTTTCAATATCATCATTTGCAAATTCCAACATTTCATAACTATTTTCTTCTTGGCTGTCGCTTTCATAAACTTCACTAAAATCATTCAATAATTCTTCTTCTCAATTATTAAAAGCAAAGTAAGGTCTGGCAGTATTTAGAATCAAAAAATTTTTATAATTAAAGTATTTAGGCTTGTTCATATTCGGGAACAGCCTTTCCTTGATCTACTCAATATAAATATAAATTTTTCCATTCTTCTTGATTATTGCATTCAAGGAATTTTAATAAATCTTGGTCAATTTGTTCATTTCATTTTGGACTTATTCCAATTTGAGTGTATTTCTTATATCATTGCATACAATAATTAATATCGTCTTGGACTTGTTCGTTGTTTAAGATATATGTTCAATTCTTTATTATTCTTTTAGTTATGTCTACGTCGTTTGGATTTAAATAATCTTCTTCTTTTAAAAAACAAGCAACGATACTAAATGAAGTTGCCCCAATCAAATATGTGTATAATTGAGCTTGCTTTATATAGCCAGGATTAACTCCATATTTTATTCAATTTTCATAATTTTTTTCCCCGACAGTTTTAATTTCAATAATTAAATGTCTTTCGGGACTATAGCCATCAGGCAACCCTCCAAATAGCTCGTTATCTTTAAAATAATCATAATTATATTGATTGGCTTCATATCTAAGCAATTTGGAATTTAATTTTTTTTCAATAATTTTTACTATTTTTGGCTCTAATGCAATACCTGCATCAACATATTTCCTGTTCAATATTGGTTTTCTAAAACCACATATATTACAAAATGCTTCAAATTGGCTGTCAAATGAGCTCAATCCCATTATAGCTCCCAACGCCGAACCGGTCATTTTTTTAAATCCTCCAAAAGTACCAGGTTTTTTTTCTTGCATAGATTTTAAGATTTTTGGATCTACTATTAAAGTCTTATTTTTATAATCCAAAGTGTAATTTTTATTGTTAAAAAATGTTCTTTTAGGAATTATTATTTTTTTTTCCATATTATTGCCCTTCTTAATTAATTACTATTAATGTAATGGTTATTTAAATTATATATTTTCAATAGTTAAAAACTAACAAAATGGGAAAAAATAGTGAAAATATAGTTTTATCAAAAAATATAAACAATTAATGTTTAATTTAAAGTAAATAAAAAATGTTTTTTTTATTAATGTTCTAACACTTAAAATTTAATTAGTATATAATTTTTTTTGTAAATAATGAAAAGAGGAATGATGAAAAAATACACTAAAATTTTATTAACATTAGGAGCTATTTCTACTTCTATTGTTGCTGTTCCATTAGTAGCAGCAGGATGCAATGATCCAAAGAATAAAAAAAACCCAAACAATAATGATCAAAGTCAAGATGGCCAAATTGAATTTCAAGAAGGCTTAGGATTAAAAATTGCAAAAATTGCCAAAGATCAAGAAAATGTTAAAGTTGATGAATTTGTAAAAGAAGCAAAGAGCGCAAAAACTCTAGATCAAATAAAAGCATTATTTACTAAATATAAAATTGCTTATGATTTAAGTGAAATTCCAGATGAAGCAAAATATGAAGTTGCTGCCGGTACACACGGCCATGCCGATGAAGGACTAGTACACTTAGATATTATTCAAACAGTGAATGAAAAGCAAAATAATAATAGATTTGAAATTAAAGGTTTTAAAAAGGAAACTGTTGAAGACACAGTCCAATTGGGAGGATATAAGTTATCAACAAAAATCCTAGATAATAAAAAAGATTTTTCTTTGCAAGAAATAAAGAAACAAATTATTGACGCTCAAGAAAAAGACTTTAATTCTTTACTTGAAAAATTAAAGGAATTTGTTAATATTGAAGTTGATGAAAATAATAAAACGAAACAATTTGAATTTAATAAAGAAAAGATTGAATTAATTAGTCAATCTGGTCAAATTGTTTTTAAAGGAATTTCTATTATTGATATTAGTAAAAAAGTAAATGAAGTCGGATATAAAACCGAAAGCAAAACTGATTTTATAATTCAAGGCTTGAACAAAGTTGAATAATTAGTTTATAATTAAGGCAATAATGAAAAACAATATTTTAATTTTATTTAAGCACATTATTGTTTACTTTTTTGTCTTGTTTGTCATTGTTAGTATTATCTTTTTTGTTATTAACTCATTGATTGAACCACAGATAAAAAAGAATATTGCAGTTTATTCACAGATAAATTCAAGCCCATGAATTAGATATGGCGAATTTTTGAATAATTTGTTTTCATTTAGAGGTGGAAAGATTTATTCAAAAGAGCTTGCAGCAACTAATTTGTCAATAATTACATTGTATTTTATGCAATTTAAATGAACAATTTTATTTACTGTAATTATTTTTATTTTTTCTTTGATTATTGGAAATTTACTAGGAATTTGAAGTGCATATAAATTTAATAAAGCACCCGATTTTGTCGTAAATATAATTGTTAGTGCTTTAGCTACAATACCTTTAATAGTTCTTGCAATATTAGCATTAGGAACATCAATTTTCATAGGATATCCAAGTCAATTTATAAATAATGGAAGTTTAACTTTTACTTCTATAATTACACCAATTATTATTACAGCATTTGGCACAATTAGTTTATTTCATGCAAGATCAAGAAAAATAACAAAAGAAACTCTTTCTTCTAATTATTATTTGTTTGGTATTTCGCTAGGAAATTCAAAAGCTAAATTATTTAAACAAAACATTATTAAAGATTTAGTTATTGCTGAATTACAAGTCATTTTACCTTTTTATATATTGCTTTTTAGCACTTCATTAATAATTGAGAGAATTTTTTCAATACCAGGACAAAGTATTTTTATTTCTTATGTATTTACAAAAGCAGAATTAAATTGCATAATGTTTTATTTTAGTTTTAATTTTTTTGCTTTATTGATTGCTAGATTCATAAATGAAATAATTCTAAATTATTTGAATCCTGCTCAAGCTATTGAAAAGCGTATAAGATGGATTAAAAGAAAAAGGTTAGATTATGAATGAACTAGATAATAAAAAAGAACTTTTTCAATTTGCTAAAAAAAGACAACAAATTCACAATTACCCAATATTTCAAAGCGAAACAAAATTATTTTGGAAGAGATTTTTTAACAAAAAGAGCAACATCGTAATATTTAGTTTTTTATTAGCATTCATTTTACTAACATTAATATTGCTATGTTTTGTAAAATTTTCTCCAATACATTCGATTGATAATAAAAGCGATTTTGTCAATAATTTACCTCCTTATTATTCTCAAATTATTACAAGAAATTTTGAAAGAGGATATCAACTTGATTTGATAAGATCTATTGAAAATCAAGAGTCAATTAGGGCTGCTAAATTAGGCATTAAACCAATATTTAGAATCTTGTATGATTCAGCAAGAGATAGCGGGGGAAGTTTAACTGTAAATACTGATATTGTTGCTTTAACATATAATCCCTACGATTTAATAAAGGCAATAAATTTGAATCAAAATTCAATAAATATCAATATTTTAAATAAAGTTTTACTTGGAACAACAGAAAGCGGAATTGATATTTATTCAAGGATATTTTGTGCATTTGCATTAACAACCTCAATTATATTATTGGCAATATTTATTAATATTTTTATTGGGTTTTCTTTAGCAGCATTTTATGTTTTTAATCGTTACAAATGATATGGAATTGTTATTGATAAAATTGCTGTTACACTTAATGCAATACCCGAAATAATTTGAATATTTTTGTTGTGTATATTTTTAGGCACAAATTGATATGGTATTTTAGTTGCTTTCATATTGATTAGTTGAATAAGTTTTTATGAACTTTCAAAAAACGAAATTCTTAATTTAATTCAAAAAGAATACATAAAAGCAGCTAAAATAATAGGTTTAAATAATTTTCAAATTACTTATAGACATTTATTTAAAAATGTCCTACCTTCATTAATTATTTTAATTGCTGATCGTCTTGCAATAAATATTCTAATTGTTGCATCCCTTGCCTTTCTAGATTTCTTAACCGCTGAAAGTAATTTAAACATAGGAACAATTTTTAAAGAAGCATTATCTTCAATAAAAGTAAATCCTCAATACTTGATAATTGTTGCTGTTTATATTACTATATTCTGTATTTTACTTAAACTTTTTTCAATAGGTCTTTCAATGACATATGATCCTAAGATTTCTAAAAATTATTAATAGAATTTAATTAGTTTTATAAAATTCTTGTTATATATCGTTAATTATTTAATTTTAATATACGAAGCTAGAATTTTTATAAGGAGTTTAATTATTATGTTGAATTTTATTTATAAAAACGATTGCAAAATCATATTTGGAAAAGATTCAATTCAAAATTTAGAAGAAGAATTGAAATCTTTAAAAGTAAAGAAAATTTTAATTGTTTATAGTGGAAAATATATTTTTGAACTAAAAATATATGACGAAATAATCAAGGCATGCAAAAAATTAAATATAGAATTTATTGAAAATGGCAATGTTATTCCTAACCCAAGAATTGAATTAGTAAGAAAATTAGTATTAGAAACAAAGGCTCAAAATGTTGATTTTATACTTGCCATCGGTGGCGGTTCGGTAATCGACACCGCCAAAGCAATAGCAGTTGGAGCGAAAAGCGATGTTGATGTTTGGAAATTTTATACATATGAAGATAAACCAAATTGCACATTACCTATCGGAGTTATTTCGACAATTGCATCTAGCGGTTCCGAATCATCAAATTGCTCAATTTTGTCAAATGAGAATCACAAACTTGGCATAGAATATGATTTTATTATTCCAAAATTCGCAATAATTGATCCTTGATATACAAAAAGTCTCCCTATGTATCAAATTTCATGTGGCATATCTGATATTAGTTCTCATTTAATTGAAAGATATTATACAAATATAGAACATGTTGATGCAACAGATTACATGATTGAAGGACTTTTAAAGGCATTGATGATAAACGCTAAATCATTAATGGAAGATCCAAACGATATGAATGCAAGGTCAGAAATATTTTTAATTTCTATTTTTGCACACAATAATATGTTAGATTCTGGTAGAGTTGCTGATTGAGCTTCGCATAGAATTGAACATGAATTAAGCAATTTTTATGGCATAACTCATGGTGAAGGTATGGCGATTGTCATGGTTGCATATGCAAAATATATGGCAATTGAAAAACCTAAAAAATTAGCTCAATTAGCCGAAAGAGTTTTTAATGTTGACAAGGGTTTGAGCCAAAAAGAAAAGGCTAATTTATTGTCAAAAAAATTAGATGAATTTTATAGAACAATAAAAATGAGAACTAGATTATCAGAATTAGGAGTAAGTTGCGAAAAGTTTCGTGAAATGGCATTAAGTGCAACTAAAAACGACAGTCAAACCATTGGTCATTATAAACCCCTTTTATCAAAAGAAATAATTGAAATTCTAAAGATTGCAAAGTAATAATTAATTTTTTTTATTTTATAAAAAACATGCCTTTTTTTAAAGCATGTTTTATTTTTTTACTATTGAAGTTAAAACTTTTTCTACAATATACTCTTCATCATTTTTTTCAAGGTTGCAAAATTTATTTAATGCAATGTTATATTTAGCAATTTTTTCATGAGATAATGCCTCATAAGATTCAATTAGTATTGAATTATTTCTATCGTAGTTCAGGCTTTCAATGCTTTCAATATTTGATATTGAAATAATAGAACTATTTAACCTTCTAGCAATAATCTCTGCTATTCTAAGATCAGATCCTGCAGCTATAATATTTGTTGTTTGACTAATTTTTCTAATGTAATATGGGCCATCTTCAAATTCAACTTTTGAAGTATCCTTTATTTCTTTAATTTCTTTATTTTCTAAATAAATTATTGATGACTTTTTTGTGTGATTAAAATGAAATTCAAATGCTCCTTTTAATTCATTGAAATCGCAAGGCCTCAATAAATTTATATGGTCATATGAATATAATATTTTAAGCTCATTGCTTAGGCTAAAATTATTTCTATTATTGATTAATAGTATAAGAATTTTTGCATTTGAATATTTTAAGACTTGCAATGTTGGCAATAAATAACCTAAATAATTAATATCTACAATGACAATTGTTTCTATGCTTGAATGCAAACCTAATCCCATTGCCATATTAATCATTGTAAATTCTCTATTACCTAAGAAAATTCCTCTTCCAAGATTGTTGCTTGCAAAAATCCCATTTAACGATTTAATATTTTGAAAATAAGAAATATCATTTGATAATACTAGTAAATTTTTGTATTTATCACATAAATTATTTATTATTTTTAAAGCATTTTCAACGTATGTTTTTTCTTTGAATTCTTCAACATCGTTAATATTTTCACGAATATTGTTTTTTAAAAAGATTTGCAAATTGTTTGAATTACTAAATGTTTTTATTTTGTTTTCTATTTTTTGCAATCTATTTTCATAAAACTCAACAACCTCAGAATACAAATCACTCAAATTTGTTTTTTGAAAAGAGGTTTTGTCTTTGAATTCTTCTAATTTTTCATGAGTTATTGAATTATCAAAGTTCTTTTTAAATAATTGTTCGCTTTGAGGACTTAAAATTTTAATAAACACAGGTTGATTAATTTTTTGTGCTTTTTTTATTGCTTTTATTATTGAGCGACATTGACCATTTGACACTTCAATGTATTTGAAATTCATCGACTCAACCTTTTTTGCTATATTTTCTGGATTAATACTTTTACTATCTACTGTGTATTCAATTTCATTTGCATCATAGATAACAATTAATTGCCTTAAATTATGAGTTCCAGCATATGCTAGCGCTTCTTGAGTGGATCCTTCTTGAAGTTCACGTTCAGTTACATATCAATAAGTAAAGTGATTAATATTCTTATCTAACCTATTTAAATAATTTTGAGCGATTGTGTTTCCTAATGCAAAACCGCCTGATATTCCTAAAAATATTTCGCCGCTTTTAATTCAATCTAATGTTCAATTCGAATATGAAATGTTTTGATTTCTATTGCTTATTAAGTTAAGGTATTCATTTTCACTAATTAAACTATATAATCTCAAAATTTCATAAAAAAGTGGCAATATGTTGGGATTAAAAAAAACAAATCTATCCCTATTGACATCAGAATTATTTTTTCTATTTATATTGAAAAAATAGCCAAAAAGAGCATGAAATATTTTTGCCCCACTCATGATTGAAGAAAAATCACTATATTTTCAACTAAATGCCATATTTAAGGAATTGATTTTTATATTGTCAATAGCTATTTGATCAATTTTTTTATTTTTCATCTTCATAACCTTGGAATATTAAATTAATATTGTTTGGTTTGGCATTTATTAATGAAAAAACTTTATGTTCAATCTTAAATATTAGTTTTTTTGTTTCATATGCAAAACTAGTTTTGTTTATTAATTTGTAACTTAAATTAATAAAAACATTTGTTTTAGTGCGATTTGGTTTAATTTCTATTTTTCCAATTAATTTTATATTTTTATTTGAACTAAAAATATTGTTTAATTCTTCATATATTGCATCTTGTTCAACAAAATAACGAAAGTTCATTTTTGTATTAACTTCAATTGCTCACATATTTTACGCTCTTCCAGTGTATTTACCAGTTTCGGTTGAAATAGTAACTATTTCACCTTCTTTTATAAATAATGGTGTTTCAACAACATAGCCGGTTTCTAGAGTAACCTTTTTTTGTGCTGCTTGAGCTGTGTTGCCTTTAACTGCATCTGGTGCTTCGACAACTTTTAAATTTACATTGGCAGGTAATTCAATGTCTAAAACCTCATTTTGAAATTTTCTTACTATTACCTTTTGTCCTTCTTTAAGAAAGTTTAATTCTCAATTAAGATGTTCCGAAGGTATTTCAATTTGTTCGAAAGTTTCTTGATCCATTAAAACTACAACGTCTCCTGCATTGTATAGATAATCCATATTTGATTTTTCAATCATTGCTGGTTCAACTTTTTCGCCACCAGTAAATGACTTAATTGTTGTTGAACCTGTTCTTAGATCTTTAACTTTTGCTTTAACATTTGCTTGGCCGCGGCCTTGTTTTGAATGCGCTGCTTCAAGCACTACATAAATATTGCCTTCTAATTTAAATGTAACTCCTGGTTTTAAATCGTTAACATTTATCATAACGTACTCCTTTAAATTTAATTAATAAGGTATTTAATCTATAAACTTAATAAATATAAATTATAATTAATTTATGGAATTTTACATTTATTATTGTTAAATTTAATGATAATTGAAATAAGGCGGGTTAAAATGGAAGATTTATTAAGAAAAGTTCCTAAGACAAAAAAAGAATTGAAGATATATTCGGTTTTAATCAATAAAATAGTTTCTTGATTAAAGCAAAAAATAAACTCCTCAAATTCTAAAGGTATAACATTAGGAATATCTGGTGGAATTGATAGTTCAACATTAGCGTTAATTTCTGAATTAGCTTTTCCAAATGGTTGCAAATTTTATTATTTAAAAACAGAAAATGATTCATATACAGAAAGCCATATTAAACTTTTGCAACTTAAATTAAAGACAAAAATTGAAATTATTAATTTAAGTTCTTGCTTTAAAAAAATAGTAAAGATTGCAAATATAATAGACCAAGGTTCAATTGCAAATACTAAATCCAGATTATTTATGACAACATTATACGGTCTTGCATTTCAAAACAAAAATCTTGTGCTTGGCACAGATAATTTTGATGAATATTATTTGGGATATTTTACAAAATATGGAGACGGTGGATGCGATTTACTGCCTTTTGCTAACATAAAAAAATCTGATGTATATGCTATGGCGAGTTTGTTGGGTGTTCCACAAGAAATTATTGATAAAAAACCTAGCGCAACATTATACAAAAATCAATATGATGAAGAAGAATTAGGATTTAAATACTCAGATTTTGAAAAATATCTATTAGATCCAACATCTGTTTCTGAGGAATTAATTAAAAAGATAAAGAATATTCACAAGCAAACTGAACACAAAAGAAAAGCAATCCCCAAAGGTCCAAAATTAAAATAATGTTCAATATAAAAATTCAAGCATCCTCACCTAAATGCTTGAATTTTTATATTGTCTTTTATGCTTTTAAATTGTTTGCTTTGGTTCAATTGCCAAAAAAATTATCTAAGTTTAATGTTTTTATTGTTGAAATGTTTAGTTTAATTAATCCTTTGTTTTCAATATTAAATAAGGGGTTGTAAATATTCAAGGTATAATCATTAATTTTTTCATTTCACAATTTAAAATTACTATTTAACTTTAAATTGATAATGCCATTAAAGTGACTTGGAATGTAAAATCCTTTTTTGATTGAATTATTATATGATTTAATTTTTAAAAAATCTTGTGTTTCTTTGCTAAAAATATATGAATCATTTAGAAAGTAATTAAAAGTATAAGAAAGGGGTCCATTTTTTTCTAAAGTATATTCTAATTTGTTAGTAATATTTAATTTATTATTTTGACTATAAAAATTAATTGAATCAAAAATTGGATTTTTTAATTCTGGATAATCATAAATAATTTTTTTGTTGTGTTGGTTTGTTTCAATAAAGCTTTGCGAATATCCTATGTGATTTCAAGGCATATTTTGTTTCAGCTGATTGAATTTAAAATATGTAACAAAAAATTCTTTAACTACAGTTGCAGAAGAATTATTAACATCTTCTTTAAAACTCTTGTCAGTAAGAAAAATATCAATATTTTTCAAGGTTCTTGTTTTAAAATCTGAGTCTATATTTCTATTATCTGATAGGTTATTTGCTAAGTCATAGTTAATAAGAATATTGGCTCCGACTTTATAGCTCAAATAATCTCTAATTTTTGTATAAGCATCAATTTTTATTATCAGTTTTTTTTCTAAAAATATAGGTTTTCCATCTTCGTATTTATCAATTTTAAAGTGAATATATTCATCATTTGTTTTATGATAAATCAACAAATTTAGAGATTTTTTATTTATTGAAATTTCTTTTTTTGTAGCAACTTCATAAATCAATCTATGAATTGAGAGAGTGATTGATAATTTATTGCTTTTTTCATCTCTATTTATTTTGATAAATATTGGATATTTTTTTAGCATCGTATTAGATACTAAAGTATTCGATTTTAAAGATTTTGAAGCAGATATTTTAAAAGTTACATCTTCACAAATATAGTTTTTTGCTAGGATATCATTTGCCTTTTTTTCAACAACTACTTGGTACAATCTTTCTTTTCTTCTTTAAAAATATTTTCTTTGAATTCACAAATCAATCTAGTTTTTGCTATAAAATACAAATAATAAAAGGGGATTCAATATTTATATAAATCGTGTTGCTTCTTTTCTTTGTCTATTTTATATTTTTCCATTGATCCTCCGGTGTCTAGAATTTGCCTTCTAGACATAATAATGTAACCATGAAAATTAAGGATTTTACCAAATTTTGGGAATTGTGAAATCTCTTATTTATTACATAAATAAAGAAAATTTATTGTCTAAAAAATTAAGAATCAAGCAATTGCTAAAATTAAATAATTTAATTTACGATAATATGATGATTTAGAAAAGAAAAAGTCTGTTCACTTAATATCATCTTTAAGTTCTTTAAAATATTTAATATAAATACATTTCAAATCATCACTCAATGATGATTCAAACAATTTTGCTGTTTTTAAAACATTCTTTTGAATTAAAAATTTAAGTGAAATTAAATTCTCTTTATTAAAAATTTCGGGAATTTTATTTATATCTATTTTAATTTTTCGTTCCCTTAACTCTTTTATTATTTCCTTAAAATAATTAAAACATTTTAAAAAGAATGCTCTGTTGCTACTACGACGCATTTTTTGCCTCCGATTAAATTGCGTTTGTATGATTTTATATCTTTATTTTTAGTAAATAATTTCAAAAAAAGTTAAATAAAAATCTAAAAAAAAGTAAGTTATACAAAAAAAACTTACTTTTAGGTTTTGTATTTTTGATTTTATAAATATTTGTCAGGATGTTCAATTTTCATTTTTTTAATAAATTCTTCTTTATCAAGAGAACCATATTTTTTCATTAATTCTTGACATTCTTTATATTCATTTTCGGCTCAAACAACATCTTCAAATCCTGTTACTAATGTAGCATTTTTACCCTTAAATTCTTTATAAGTAGAAAAGAAATTTCTTACTTGAACTAATCACATTTGATCGACATCTGCTAATGATTTAATATTATCTAATCTATAATCATCTGCGTGAACTGCAATTAATTTAGTATCTGTTTCGCCACCATCAATCATTTTCATTGCACCAATTAGTCTTGCATTTAATGATGTTCCGGGTAAAAAACTTTCTGAAGAATAAACTAATACATCCAATTCATCTCCGTCTCAATCTAGTGCATCAGATAAATAGCCATAATTTGCAGGATATTTAAAATCACCTCTTAAAATTCTGTCAACTTTGATTTTGTTGCTTTTGCGGTCATATTCATATTTAATTTTTGAATCTTTACTAATTTCAATGTTTACTTCTAAATTTTTCATATGCTTATTTTATCACTTTCATAGAAATTCCTTATTGTTTGGGAAAAAAATAAGAGTTTTCGCTTATTTTTCCCAAATGAAATAATAGTCAGAAAAATCCAATAAACTTAAAAGTATGAGGAGTAAAAATGAAAATATATTTATATGGAAAAATAATGCACGTAAACACCAACTATTTAATTTTAGATCATAATGGTGAGGGTGAATTGATATACGTGGCAGATATAAAAAGATTTAATAAGGATGATTTGAGAAAAATATTTATTTACGATGTGGTAAATGAATATAAAAAAACTACTTATGGATTTGAAAATTTTAAAGAATTAATAATTTTTGAGGATTTAATTTCATTACAAGGTTTAGGACCCAAGACAGCAATTTCTCTTCTAAATTTAGGATGAGAAAATTTAATTCAACTAATTGCAAACGAAGATAAAGATAAATTATGTGAAGCTTCTTATGTAAGTTTGAGGATTGCAAACAATATAATTTTTGCCTATAAACAAAAATATGCAAAATTTTTATCAAAATTAAGCGATGAAGATTTACTTAAAATCAAGAGCAAAAACACAAGCGACTCTTTAAGAGAAAAATTTGAATATACAATGAGAATGCTCGGTTTTAAAAAACAACAAATTTCATACGCATTAGAAAAAATGAGTATAACCGAAAATATTGAAGAATCAGTTGAAAATGCAATAAAAATAATTGGAATGAAGCAAAATGAGTCAAGAGTTCTCGATCAATAGTTTTGATGAATTTGTAGGCCAAGAAAAACTAATTTCCACTATTAAAATAATAATAGAATCTGCAAAGGTTCAAAAAAAGCAAATTGATCATTTACTATTTTATGGTCCGCCAGGTTTAGGAAAAACTACACTTGCTAAAATAATTTCAAAAGAAACAAAAAGCAATATTGTTTATGTTCAAGGCCCACTTATTGAAAAAAAGAGTGATGTATTGACTATTTTTTCTTCAATAAATGATAATGACATAATTTTTATTGATGAAATTCATGGCATAAACAAAAATATTGAAGAATTATTTTATTCAGCATTAGAAGAAGGAACAATTGATGTTGCGTTGGGCGTTGATGGTGATAAAAAAATCATGAGAATGAAACTAAAAAAATTTTGTTTGATTGCTGCCACAACAAAAATCAATTTATTATCCAAACCATTAAAAGATCGTTTTGGCTATATAGGAAAATTAAGCGATTATAGCGACTTAGAAATTGCTAAAATAATTTTTAACTCTGCAACTAAAAATAAGATAAAAATTAAAAATGATGCGATTAATTTTATTGCAATTCATTCAAGGCAAACACCAAGAGTCGCTAATACTCTTTTAAAAAGAGTTAATGATTTTGCCATATATTACAAAATTGATCTAATTGACTTAGAAACTGTTAAGAAAGCCTTTTCGTATTTGGGTATTTATTTGTATGGTTTGTATTCGCCTCAAATTGAATATTTAAAAACATTACATAAAGTATTTAATGACAAATTTTCTTCGCTTGATGCGATAAGTTCAATAATTAAGGATGATAAATATACTATTTTAAATGATATTGAGCCTATATTATTGATTTATAAGTTAATTGAGAAATCACCCCGTGGTAGAAAAATTACAAACAATGGCATTGAATATTTAAAGAGTCAAAATATAATTACTTAATTACTAAGTAATTTTAATATTTTAGATAACATGCATTTTTTAGTATAATTATTAACTATATGAAGAAAACAAAAATTTTAAACAATAAATTTAGATGAGTTATTAGCATTTTTTTAATTTTAATTTTATTGATTGTGACAATTTTGGGATCGATTTTTTATTTGTTTCCGCAACTTAAAAAACAATCCAATTCAAATGTTACATCTGCTAAAATTGGGTTAAAAATAGAAAGGGAAAATCAAAAAGAAGACTCGTATAAAAAATCATATGGATTATCAGATCAAGAAATTTTGAATTTGACTAAGAATTACATAGGAAACCAAAACGGGGTTTTAAGTACTTCATATGATATTAATTTGGTATCTAAAAATTTGATTTCTGCTACTAGTTATCAAGATAAAACAGATAAGGATGTTAATAATTTAATATCCAATTTTGTTAATAAGCCTTATTTAACAGTAACTGATGGTGGCGGTAATCCATTATTTTATAAAGGTGAATATGTTTCTAGGTTTTATCCAAATCCTGATGTTCCTAGAACTCTTAAAGATTTTTTGAAAAATGGACCTCAAAACTACAATATTAGCGTAGAAAAAAATCCAGCAACAACCTACAACAAATTTGGTGCAAGACAAAAAATTCAAGTTAAATTGGATGAATATGGATGGGATAGTTTTGTGCAAATGATAAACGAGTACATGCTTGCAACAAATTTATCTTTACAACGTAATAGAAAAGCCTCGCGTGAAGAAATTATAAATAACCCAGGCAATAAAATTTACTTTTGAATGAATTTGGCAGAATTCATTCGCAGAGCAAAAACCGAGTTTCCAGATGAATGAAAGGCAGCGGGAGAAAATCCTATAAACTTTGCTTATGTTGGAAATTCTGCTTTGCCTGAAATAAAAAAGGACAAAAAAGGGAATATCATTGATATTAAATATCCTGTTTTAAAATCAAAAGAAATTAATGCAAGAAAATATTTGATATCAGCAGTCAACCCCGCAACATCTAGAATACTTTCAACTGAAAAAAATGAATCATCATTTTATTTAGCGAACGAAAATTTAGAAGGTTTAAGTGATGAATATATTGCTGCTGCAATTAACTATTCATATGCCCCATTTAAATTAACTAAAACCTACAACTATTTTTTAACCGGTAGTTCAACAAATCAAAATAGATATTTAGCAGTTTTAGCAATTCTATTTTCGTTATTTGCAGTATTTTTGATTGCTAAATATCGTTTATATGGAATTATATCTTCAATATGCTTGGCATTTATGATATTTGTGTTCTTATCAATTATTACTTCATTTAACATATATATAACAGGAGCCGTGGCATTTACTTTCTTAGTTTCTACATTCTTAACATTTTTAATGATTTGACAAATATTAAGACGTTTAGATAAAGAAGTAAATTCTGGTTCTAATGGAATAAAAGCAACGAATAAATCATTAAGATTTGGAATAATATCGACACTTGATATTGCCTTTGCATTAATTGTAGTTTCAATATTTACTATCTTTATAAAAACTTCATATTTAAATGCGATGGGCATAATGCTATTTATTGGACTAGTTTCTGGATTAATTATTGGAGTATTGGTTAATGCGTTAATAATGTGAAATTTAATAAAAACGGAAACGTTTGATAAAAGAACAAATTGAATTGTTTGAAATAGCAAAAGAGCAAATTCTTTGATAGCAAAAATTGATTTAATCTCAAAATCAAAATATTTTAGTATAGGTTTTGCAATATTTGTAATATTAACCATTGTATTCTTTGGAATATATAGCGGTGTATTTGGAAATACAAAATTAGGAATTAATGCTAGTGAAACATTAGCGAATAATTACTTATATTCTATTCAAAAATCATCAATAAATGGCGATTGAAATAAAACTGAAGTTGATCAAATTATTAAATATTGAAAAGAATTAAATATTTCAAATGCAAATATTTTCTTTGAAAAATCAAGTTTAACTAACGACTCATATAATTTATTAATTTCTTCAGCAGACAAGATTGATTCAACTAAATTAGAAGCTTTAAAGACATTTATTAATACTAAAATTTCAGCTATAGGTAATGAATTTGATTTGAACATACACGAAATATTATTAAATGCAAATGGTGAATTAATCAATTTTGGATTTACAATTCTAATAATTGCGGTTTCATTAATAATTTGTGGAATATATAGCATTATAAGATTTGGTTTAATAGCGTTTTTTGTAATGTGAATAAACGAAATATTAGCAGTTATAAGTTCAATAGTTTGATTAATAATTTGCCATGGACAATTTAACAATGGAATTTTAGGAGCATTGATATTATCTACTGCATTTAGTGTAGTTGATTCTATCTCTAATTCTCAAACAATTAAGGAAGAATTTGGCAATAATTTAAATACAAAAAATTACATATTTGAAATTGACCAAATAAACAATATATTTAAAAAATATGTAATAGAATCTTTGAATAAAAACATCTTCAATATATTCTTTGCAATTATTTTTGTGGCATCTACATATACGTTGATGACAGGCTTTAATGCAACAACAATATTAGTAACTTCAATGTTTATTATTTCAAATTCATTTATCAATTTATTTATATTGCCAAACATTTGAAGATTGATGTATATCAAAAGATATTCAATCAAGCAAAAGAGAATTGAAACTAAATATTGAGAAACAGAAAAGATTAGCGAACAAACATTTGTTGGTATTAATGATTTTAATATCTAAAAAAGGAGGAACCAATGTTTCAAAGACTTAAAGGTACAAGAGATATATATGGGTTAGAAGAAAAGGTTTTGAATTTGATAGAAAATAAATTTTTTAAAATTATAAAAAATTACAATTTTAAAAGAATTTGCACACCGATAATTGAAGACGCTAACTTGTTTATAAGATCAGTTGGTGAAACTAGTGACATTGTTTCAAAAGAGATGTATCTGTTTAAGGATAATGGCCAAAGAGACATTGCCTTAAGACCAGAAGGAACCGCTTCAACAATAAGAGCTTTTGTAGAAAACAAAATAAATAATTTAGAACCTACTAAATTAGCCTATTTTGGACCTATGTTTAGATACGAACGCCCTCAAAAAGGTCGCTATAGACAATTTATTCAGGGAGGAGTTGAATTAATTGAAAATTCAACTATTGAGAATAATTTTGAAATAATAAAATTGGCATATGATTTTTTAAAGTCGTTAAAAATTAGTAACATTACTTTAGAAATAAATAATTTGGGCTCTTTTGAGTCAAGAAATAACTACATAGAAATATTAAAAGAATATTTTTCTAAATACAAAGAACAATTGAATGAAATTTCTTTAATTAGATTGCAAAAGAATGTTTTGAGAATCTTAGATGATAAAGAAGAAATAAAAAAAGATTTTGTAAAAAATGCTCCAAAATTAATAGATTATTTATCTTCAATTGAAAAAGAAAGATTTAAAAATTTACTAGATTTACTAGATGAATTTGAAATCAAATATGTAATAAATCCCTACTTAGTCAGGGGGCTAGATTACTACAATGATATTGTTTTTGAATTTGTTTCAAATTCAGACGCCCTAGGTTCGCAATGTACCGTTCTTGGGGGTGGAAGATATGATGGTATGATTAAAAGCTTTGGCGGGCCTGATATAGGCTCTATTGGGTTTGCCTTTGGAGTAGATAGATTAATGGAAATAATAATGTCAAATATTGACAATTATCCAGAGCTAAATTTTAATGAAACTATATTAATTGCTTATTTGAATGAAGATGAAAAAAATGAAATAAGAAAAATTGCTTATGATTTGAGAAAGGATTTTCAAGTAATTTTTCCAAGTCAAAGACAATCAATTAAGTCTCTGTTTAGACTTCAATATAAACTACAACCAAAATATTTAATTTTTAAAGAATTAAATATGAAAAAAAATGAGTTGAAAATAAAATCAGTTTTAAATGAAAGAAATATAATTTATACTAATTTAGACGAATTTAAAAATATTATTAGTGAAATGAAAAGAGATGAAAACAATGAATAAAAATTATTGTGGAACATTATGTATTAATGATTTAAACAAAGAAGTAGAGCTTTGTGGATGAATTGCAAATAAAAGAAAATTTAAGCAACAAGTTTTTATTGACTTAAGAGATTCAAGTGGCGTAGTGCAATTAATATTTCAAAATGTATCAGACCCTTTATTAACTAAAGAAAGTTGTATTTATGTAAAAGGAACTGTTCAAAAGCGTTTAGAAGTTAATCCAGCATTAAAAACCGGAGAAATCGAAGTTATAGTTTCTGATTATAAAATTTTTAATTCAGCAAAGCAAATACCTTTTGAAATTAACAATTCAAAATCAACAAATGATGCAAACGAAGATATAAGATTGGAATATCGTTTTCTTGATTTAAGAGAAGAAAGAATGCTTAATAATTTAAGATTAAGACATAAATTCTTACTTTTGGTAAGAAATTTCTTCGATAAACAAGGTTACATTGAAGTAGAAACTCCAATTTTAGGTAAATCAACTCCTGAGGGAGCGAGAGATTACTTGGTTCCTACTAGAAGAAAAGGAAGATTTTTTGCATTGCCCCAATCACCACAACTATTTAAACAATTATTGATGGCTGCCGGATTTGAAAAATATTTCCAAATTGCCAGGGTTTTCAGAGATGAAGATTTAAGAAAAGATAGACAACCAGAATTTACTCAATTAGATATTGAAATGTCATTTGGAAGTCAAGAAGAAATATTTAAATTATGTGAAGAAATGTGAAAGGATGTTCTTTCACAATTGGGGTACAAAATTGAAACTCCGTTTCCAAAAATGGACTTTTTCTATTCGATGGCTCATTATGGAAATGATAAACCAGATACAAGATTTGGATTTTTAATCGAAGATTATTCAAAACAATTAGCAACTAAATATAATAAAAAATTTGTAAAAGCTATTGTTTTTGACAGAAATATAAATGAACATAAACAAATAATTAATGAAACTTTTAAAAAGAATAATGGACAAATATTGGATATATTTGATTTAAAAGAAGCAAACAAAAATGAATTCCAATGTCTTTTAGATAAGGCAAAAGAAGATAATATTTCAACCCCATGAGCAATAATTTCTGCTAATGATTTAGAAGAAGATGCATTGAAATCACTTGGAGCAATTAGAACAATTGCAAATGATTTATACAAATTAGCAGATCCAAATCAATTGAATTTCTTATGATTGGTAAATTGACCTATGTTTGAATATGACAGAGAAACAAAAACATATTCACCTGCTCACCATGCGTTTACACAATTTGAAGAATCTACAATGAAATATTTAGAAACTGGAGAGCTTGAAAAAGTTAGAGCTAAATCATACGATTTAGTGTTAAACGGATTTGAATTAGGATCTGGTTCTATAAGAATTTATGATCCAAAGATTCAAAAGAAAATGTTCGACGTAATTAATCTAAGTGAAAAACAACAACAAGATCGTTTCGGATTCTTTTTAAAAGCATTTGAATATGGTCTACCTCCTCACAATGGTATTGCATTTGGAGTTGAAAGAATTTTAATGATTTTAACCAAATCAGAATCAATTAGAGATGTAATTGCATTTCCTAAAAATGCTAAAGGTATTGACTTGTTGACATCAGCTCCTAGCGATGTTACAAAAGAGCAATTAGATGAATATGGATTGGAACTTAAAATTAATAAAAAATAATAATATATAAATTAAAATATATGGGTAATAGTTAAATTAATAATTTGATTTTAATTATAAACAAAACAACATAAAAAAAGAAAGGGATTTTATGCATGCTGCTTCAATAGCCTTAACAGTTATATTGGTAATAATTTCATTTGCGATAATTGGAATTTCATTTATTATGGCTCCTGATTCAAATAGTTTTAGCGGAGCTTTAGTTGGTTCAAATGACCTAGACTTGTTCAAAGTATCAAAAGAAAGAGGAATTAAAAAAGTATTAAAGTGATCAATGATTACTTTAGGTATATTACTTTTTATATTCTTGATCGCTTTAAGAGTTGTAATACAAAATGGTTAGAATTTTTAAATAAAGATGACATATATAAAAAATTTTAAAAATAAGAATAACAACCCTAAAATTAATGAAGAACAAGTATTAAAATGCATAAGAGAAAGTGATGGAATTACTTTTATTGAAATTGCAAAAAAATTAAGAATTCCTGCTTTTTTGAATTTTGAATTATCAGAAATATTAAATAACTATTTATCTAAAAAATTAATTGATACGAAAAGCGATGAAAAATACATCGCTATTTATTTTCTAACTAAAGTACAAAAGGAAATATCAATAACTGCCAAAAGATTAGGATTTATTGATTTTGAAATGCAAGAAGAATCTCAAACTAAAGTATTTAGTGCCTTTCTTGCGCCTTTTCAATTAAAAGCGGTATTGGACAAAGATTTATTGCTTGCAAATATTTATTATTATTATGAAAATGGCATAAAAAAATATAAAGGCGATATTATTCAAATTTTAAATCACGCAAGAAAAACTATTGTTGGAACTATTGAAAAGAGCGCAAATAAACTGTTTTTTAATGCTTATGATGAAAAAGATAGGGCTAAATTTGAAATTATTAATGAAAAAGAATGCCCAGATATAACAAAATTGAAAAATTCATTGGTATCTTGTGAGATTTTAAAACCAAATAATAAAAAAGTTGCTATTGCATATAAAAATATAATTGGTAATATTAATGATAAAGAACATGTGATTAAAAAAATCATTGCCCAAAATGATGTAAATGTCAATTTTCCACAAAATTTATTAGAATATGTTTCCTCAATACCTTCTTTTGTTTCACAAGAAGAAATTAATTCAAGAATAAATTTAATAGATTTAATGACAGTTACAATTGACGGTCTTGACACTAAAGATTTTGATGACGCCATTTCGTGTTATAAAAATGAAAAGAATAATTGAAAGTTATTTATTCATATTGCCGATGTTTCATACTATGTTAAGGAAAACGATTTAATAGATAATGAGGCTTTATTGCGTGGCACAAGCATTTACTTACCCGATAGGGTTATACCAATGTTGCCGGTTGAATTGTCTAACGGAATTTGTTCATTAAACCCAAATGAAGTCAGAGCTTGTATAACATTGGAATTAGAAATAGACAAGCAAGGGAACAATATATCATATAAAATTTATCCTAGTGTTATAAAATCAAATTATAGATTGACTTATAATGAAGTTAATGATTTTTATAGCAATATAAAAAGTGTTCCTGATGATGTTGCAAATATGCTGTTGCATGCACGAGATCTTGCAAAAATTATTAGAAGTAAAAAAATATCCGAAGGTTATGTTGACTTTGAAATTCAAGAATCAAAAGTTATAATGGATGGAACAAAGGTTGTTGATATCAAGGTGAAAGAAGAAGGCGAAAGCGAAAAATTAATTGAAGACTTCATGGTTCGGGCAAACGAAACCGTTGCTCAAATGATGACTAATTTAAAAATTCCTTCAATTTATAGAATTCACGAAAAACCTTCACCTGAAAAACTAACTTTATTGCAAGAATTATTCAATTTTTGTGGATTTAAGGATGTAAAAGTTCCTTATGATGGAGAACCAAAATCATTTGGAGAAATGATTGCAAAATTAAAAGAACATAAATTTGATGATTTTATTAAAATGGCATTATTAAGAACAATGCAAAAAGCAATATATTCATCAAATAATATTGGCCATTTCGGTTTAGCTTCAAAAGCTTACTCACATTTTACTAGTCCAATTAGAAGATATCCAGACTTATTGCTACATAGATTAATAAGAACTTATATTTTTGAAAAGAAAGAAATAACACCCGATTTATCAGAATCGCTGAAACAAAAAATAAGTCAAATTGCTTTAATGAATAGCGAATCTGAAAAAAGTGCAATGGTTGTCGAACGAAGTGTTGTTGATGTTAGAAAATCAGAATTCTTTGAAAATTTAATTAATAAGCAATTTTTTGCAACATTAGTATCAATAGAAAAATTTGGAATGTTTTTTAACATTGACGAATATCAAACAAGTGTTTTAGTTCGTTTTGAAAATCTCACGATTCCAACTAATAAAGACAACGATTTTCAAGCATCATCTAAAAATAAAATCTATAAAGCAGGCAATAAGTATTTAATTACTATCACTTCGATAGATCACGAAAAAGGAAATGTAAATGCAACATTAGCTTAGCATTGACATTTTTTTATTAATTAATTAAAAATAATTTTAATAGTTATATTTTTTTATAAAATAATAGTATGCCCAAATTGATAATTTCAAATAAATTTATAAAATCAAATTATGAAATTGATTCCACATATGAATGTGGCATTTCGTTATTAGGTTGAGAAGTCAAGAGTTTAAGGGCAAAAAATGCAAAACTTGATAATTCTTTTTGTTCAATTAGTAATAACCTAGAATTATGAATCAATAATTTAAATATTGCTCAATATATGCAGGTAAAAGGCGATTTAAAAAGAAGTAGAAAATTGCTAATGCATAAATCAGAAATATTAAAATTAAAAAACAAGCAAGAAAAATTTTCTTTTGTTATTGTTCCGGCTTCGATATATTGAAGCAATAATCATATTAAGGTAGAAATAGCTTTAGCTAAAGCTTTAAAAAAATATGATAAGCGAGCTAAAGAAAGAGAAAACGAAATAAAGAAACAAATAAAATCAAGTTATTAAGGGGGCGTACAGGCTTCGACATACATGCAAGTTATTAAGTTGCAGTAGTTTGGCAAACTATAATGCTACTAGGCTTTTTAAGCGCAGAAGAAAAACAAAACAAACAATCATTTGTTTTAAATCAAATGATGTCATCAAATCCAGTTTTTGCATACTAATAAATTCTGGTAGGCTAATGAATAGCTCGGTTCATTGCTAGCCTTTGGAGAGCTCAGCAATTAAAGTGGCGTTTTAATTGCAAAATTATGCCAATGGTTATTATGAAGATTGTTAATTTGATAATAATATAACCAAACATAAAATTAACTAAACTGTAGATACTTAATAATGAACATGTGTGGACCGGGGTTCGATTCCCCGCGCTTCCACCATATGAAAAATAAAGAGATCAGCGTTTTATGATCTCTTTTTCTTTTAATTTGGTGATATTTTTTTAAAATAAAAATAAATAATAAAAATTATTTTTATTTTTTATAAAATTAAAATAATCTAGTTATTAAAATTAATAAATTAAATATAGTTTTATAAAACTTATTTAACTTTAATATAATTTTAACTATAAATATATAAGGAGGGGAAATGGCGGTCAGATTAGTAGTAAAGCATGAAGATGGTTGGGCAGTCAAAAATCCAAAAGGAAAAAGAGCATTGAGAGTATTTAAGACTCAAAAAGAAGCTGTTCAATATGCAAAAACTTTGCAAGATACTTCATCTGTAAATGTTCAGTCTAAAGCTGGGAAGTTTAGAAAGTTCACAAGCTAAAATGGCAATATTATTAAATGGTAAAGCAATCTCTTTAAAAGTTAAAGAAGATTTAAAAAAAGCTATTGAAAAAATGCCTCAAGAAATTAGACCTACTTTAGGAATTTTACAAGTAGGCGATTTAAGTGAGTCAAATATTTATGTAAAAAATAAACTTAAATATGCTTCTGACATAGGATTAAATACAAAACTAATAAAATTGCAACAAAATGCAAGTAAATTGCAAATTTCTGAAGCGATAGAATTATTAAATAAACAAAGCACCGGATTTATAGTTCAAATTCCAATGCAAACTAATCAAATTATAGATAGTCAAGAAATATTGGATTTAATAAGCATAGATCACGATATTGATGGTCTAACCTCTTTTAATCTTGATTTAAAAAATCCTAATAATTATTTTTTATCAGCTACTGCTAGTGGAATTTTTTTACTTTTAAACAAATACAATATTCTTTATAAAAATGCAAAAATTGGAGTTATTGGTCAAAGTAAAATTGTCGGGTACCCATTGGCAAATTTCTTAGTTTTAAAAGGATATAACGTATTTAGATACAATAAAGATACTCCTAAAAATAATATTAAAGACTTGGATATAATAATTGTTGCAACCGGCGCAAAAGATCCAATTACATACGACCAAGTAAAGCCTGGGGCGGTTATTATTGATGTTGGAATACACAGAGATAGCAATAATAAAATTTCAGGGGATTTAAATTTTAATGAATTTTCTAATGTTGCCTCATTTATAACCCCTGTTCCCGGAGGGGTTGGTCCAATGACTATTTCGGCATTGCTCATTAATCTTTTAAAAGCAAGTTCCTTGCAAAATCCAATTATTGTACCATATCTTAAATTTTTAAAATCATATTTTAATTAAAAATTTTTTAGCTGATTTTGAAGGCTAAAAAATTTTTTTAAATTTGATTTATATTATTATTTTTTTGTAAAATAATATCACATAAAACAGGAGGGAAAATGATTATAGGTGTTAGTGGCATGATTGCTGCCGGTAAAAGTTCATTAGCTGAAAAACTTCATCGTTATTTTGAAGGTTCGCTTATGCTTCATGAATTTGAAGAAGACGACGAAGTATTTAATACATTTTTGAAATGACTTTATGAAGGGAAGCCTAATTTAACAATAGGATTTCAATCATATATAGTTGAAAATCATTCTACAAAATTTAATAAAATTAAAGAAGAATTTGAAAAAAGCAAAAAAGATCCAATTGAAGATATGATTTTTTTGGATAGATTTTCAATAGAACATTATATTTTTGCAAAATTAATTTTATCTAAAAAAGGTAAAAGATATTTAGATGCATATGATGCTTTGTTTGAAAAATTAATTAGTAAAGATGAATTGCCTGATTTTGCAATATTTTTAGATATTAATTTTGAAACATTTAAAAAGAGAATATTTACACGCGGAAGGCAAAGTGAAGTAGATAACTGAGATGAAAACTATGAATACTTCCGTAATTTACATGAAAACTATTTTAAAATTTTTAAAGAAATTGCTCAAAAATTTGGCTTAAAATATGTTGTAATTGACACAAATAACTTAACAGAAAAAGAAGTTTTTAACAAAGCTTTGGACATTATTGAGAGTGAAAAAGAAAAATGTCGCAAATAGACTATAGAATAAAAAAAGTTCTTTTTGATCAAGCAACATTAGAAAAGCGCATTAAAGAATTAGCTAATTGAGTCAATGAAACATATAAAAATTCAAAAAATTTAATCATAGTTGGTTTATTAAAAGGCTGTTTGCCATTTTTGGCGCAGTTAATTAAAGACGTAACTGTAGATTTTGTTTTGGATTGCATGATAACTAGCTCATATGATGGAAAAGATAGATCTTCGGGAAATGTCAAAATAACATTGGACTTACAAAATAATATTGCAGATAAAGATGTATTAATTGTTGAAGATATAGTAGATAGTGCTAGAACTATGACCAAAGTTGTTTCACTGCTAAAATCAAGAAATCCTAAATCATTAAAAGTTCTTACTTTACTTGATAAACCATCTGGCAGATTAGTAGAATTTAAACAAGATAACTTTGGTTTTGAAATCGGCAAAGATGACTTTGTTGTTGGCTTTGGCTTCGATTGAGAAGAAAAAATGCGTCAATTACCATACATTGGTTTATTGACAGATAAAGAAATTAATAAACACAAAAAACACTAATAATTTTTCAATGATTCTTAAGTTACAAAATTGGCAAATAACAGAAACTTCAAATCAAGATAGTGGCACTAGTATACCTTTAGCTATTGGCTCTGCGTTTGTAGCATTTATTTTAATAGTTGCTGTAGTTATTTTTATAATAAAAAAAATAATAAATAAGCAAACAAAAACAAAAGATAATTCAACTAATAAAGAATTAAATAAAGACAATAAAAATGACGAATATAATAAAAAAATTACATTGCCTTTTTTTAATTCTGAAGACCTAAAAAGAATAGATAAACTAAATTCCTTAAAAATTGAAAAACAAATTGCTTATTTATTTGACCCACAAAAATTTTTTAAAGAAAATTTAAATAATTTATGAGTTAAAAAATATTACGAAATTATTAAAAAATTTAAATTAGAAAAATCGCCTACAAGTAAATTTAATAAATTAAAAATCAATAAATATTACAAAAATTATCTTAATGAAGTGGGCCAATGAAATTATTGCAGACACCACATTGATGAAATAAAAATAAGTGGAGCGATTTATAGTGCTCATCCTCAAACAAAAGAATATTATAAAACCGGTGATGTTATATTGGTAACATATGAACAACACTTATTTTTACATTATTTAATTGTAATGGCAAAAACTACAATGCCAAATCACGGAATGATAGTGGGACTTAAATTTCATTTTAATGATGCTAAGAAATGTATTGAATATTGAGATAAAGTTATTCAAAAAATGTGCGAAAAATATTTAGTAAAATATGACAAAAATTGAAGTGAAAATTTAACTTAATTTAAATAGTGAGTATTCACTATTTTTTATTTTAAAAAAACTAATTTTCTAGATTTATTATAATTATTAATATAAAATATCAAAAAATATTAATTGCAATTTTAATTAATAAGAATTTTTAATTTATTAATAATTTTTAAGGAAAAATATGAAAATAAAGCTTTTTAATAAAATAATAAATGATGAAGATAAACCTAAAAAAACTCATTTAAGTTGAACAGAAAAATTTAAACTTTCGTTGCAAAAAGAAAAAGACAATTTTAAAAATTCAATTCAAAAAACAAAAGACTTTTTTTGCAATCCTAAAAAGTTTTTTAAAGATTATAAAGAAAATGTTTTGAAACCAGAATGAAAAGGATTTGTAGAAGGTTTTAAATTTGAAAATTGGCCAATTGTTCAAGCAATAAAAAGACTGAAAAACCCTAAAAATAACATTAAAATTAAAGAAGAAAAAATTAAAATAGCAAGATGAAATTTTACTGAAAATAAAGACGAAGATCAAACAAATGATAAATAAAAATTTGCTAAAATTTTAAATTTTTTAGGTTTTAAAAAATATTTTATTACCTAGGCTTTTTATAGCTAATTACCATATTTATAAATGTTGCATAAAATATTAAAAAAATTATTTGAAATTAATTTTTTAATAGTGATAAAATCACTATTTTTTATATTTTAAATAAAATATATTAACCTTAATTTATTATAATTATTAACATGATTGAAATTAAAAAAATATCTTATAAATATCCAGGTTCATCGAAGCTGGCGTTAGAAAATATTGATCTTAAAATTGAAGAAGGTCAATATATTGCTATTTTAGGTCATAACGGTTCGGGAAAAAGCACATTTTCTAAACTTTTATCTGCAATTTATAAACCAACATCAGGAAAAATAATAATTGATGGATTAGAAATTAATTCAGAAAATTTACTAGAAATTAGAAAAAAAATTGGAATCGTATTTCAAAATCCTGATAACCAATTTATTGGTTCAACAGTTGAAGATGATATTGCTTTTGGTCTTGAAAATAAAATGTTGAAACCTGAAGAAATGAAAACAATTATTTATAAATATGCTCAAGAGGTCGGAATGCTAGATCAGTTAGATAGAGAACCACAAAATCTATCTGGTGGTCAAAAACAACGCGTTGCAATTGCTTCAACATTAGCCCTAGATCCTAAAATAATTATTTTTGATGAAATTACTTCAATGCTAGATCCTAAAGGGCGTAATGATATTTATAAAATAATTCATAATTTACACAAAAAAACTAATAAAACATTAATTTCTATCACACATGATATGGATGAAGCACTACTAGCAGATAAATTAATAGTATTTGCTGGCGGTCATGTTATTGCTCAAGGTAAACCAATAGAAATCCTAAATAACAAAGAAATTATTGAAATTGCAAAAATAGATTCTCCATTTATCTATAAACTAAGTAGTCTCTTAAATGATATTGAACCAACATATAATAGAGAGGAATTAATTAAAGAAATATGCAAATAAAAGCTTCTGAAATTACCAAAGAATACGACAAAGGATTGCCCACTTATATAAAAGTTATTGATAATGTAAGTTTAGAAATATCAGAAGGAGAAGCAATATCCATTATTGGCCCAACGGGTTCAGGGAAAACAACTCTTATTGAACATTTTAATGGTTTAATTATTCCTACAACTGGAAAAATTATCTTTAATGAGCTGCCAATAAAAATTAAAACTCCGAAACCTAGAAAACCTAACTTAAAAAAAATTAAACATGATTCAAATGAATATGAAATTGTTCTTGAAAAATGAAAAAAAGATCTAAAAGATTGAAAATTAAAGAAAAAATCCGAAAGGTTTGAGTGAGTTACAACCGATTTAGAGATTTCTAAAACCATAAGAAAAATAAAAAATATTAAGGCATTAAGAAAACAAGTGGGAGTTGTATTTCAGTTTGCTGAATATCAACTTTTTGAATCAACCATTGAAAAAGATATAATTTTTGGACCTATTTCAATGGGTGTAAGTAAAGAAGAGGCTAAGAAATTAGCACTTAAATATATTGAAATGGTTGGATTGCCTATTGATTATTTGCAAAGAAGTCCATTTAATTTATCGGGCGGTCAAAAACGAAGAGTTGCGTTAGCTGGTATATTAGCAATGGAACCAAAATTTCTAGTTTTAGATGAGCCAACTGCCGGGCTTGATCCGCAAGGTGTCGAAGAAATGCTAAACTTATTTCACAATCTCTATTTAAATGGAAAAACGATCATTATTGTAACACATGACCTTGATAATGCTCTAAAATGAACAAATAGAACAATATTTGTAAAAGATGGAAAAATAATTAGAGACGGAAATACATATGATATTTTGTCAGACGACAATTTCTTAATAGAAAATAATTTAATTCCTACTAGATTATTATCATTTGTTAATGAATTAAAAAAGAATAATGTAGATGTAGGTAAGGTAACAAGTGTTGAGGATTTAGCTGAAAAAATTAATTATTACTTAACCATGAAGCGCAAAGGAGGAACAAATGAATAAAACAATTATTGGTAAATATTTAAATTACGATACGTTTATTCACAAAATTGATCCTCGTTTCAAATTTTTAACAAATGTTTTATTAATAATATTGCTTTTTATGGCTGATCACTTTATAACTTTGGGAATTTTAATTTTCTCAATAATGATTTTTTATTGAGTAGCCACAAAATCATTAAAATCACTATTTTCTAAGTTGCGCTTGCCACTATATATTGCAATATTTCTATTGATAATAAATATGTTTACAGTTAAAGGAGCAGTCTCTTTAAATTCGGTTTTAAGTTATGTTCCACTTGCTTCTGATGGAAATTTAGACTCCTATGTCTATTATAGAAATGAAACAATAATTAATACAATTTATTGAGCCCCATTTGGAAGTGAAAATATATTTCAATTAACCAAATTTACAATTTATAGAACGTTATCAATATTTTTAAGAATATATGGTGTTATTTTAACAACAACAATTTTAACAGTTTCAACAAAACCAGTTTTACTAACTAGAGCAATTAATGATATTTTAATCCCCTTAAAGTTAATTAAAATTCCTACAGAAATTATAACAATGATTATTTCAATATCATTACGTTTTATCCCAACATTACTTGAAGAATCTGGTCGTATCATGAAAGCGCAAGCAAGTAGAGGTATCGATTTTAAAAATGGAAATTTAAAGGATAAGACTAAATCCTTTATTGTTTTGATTGTTCCACTATTTGTTTCTTCATTTTCAAAGGCGAATGATTTGGCTGACGCAATGACATCAAGAGGTTATGAACCTTATGCTAAAAGATCTCACTATAGAGTGTTGACACCTTGTTGAAGAGATATAATTGCTGTATTAGTCATAGCTAGTTTAACAACGTTAGTTTTAGTGGCTCAACTTGACGCTGTTCCATTGCCAAAATGATGATTAGTAACATTTCAAAGAGTTTAATATGAATAGCAATAACGATATAGAAAAAATAAGAAAAGAAATTTTTGATTTGCAGGCTAAAATTAGTGCTTGAGATAATGCATATTATAATTTAGATTCGCCTGAAGTTCCAGATGAAATTTATGATGTTGAATTTAATAAATTAAAGGCGCTTGAAGAAAAATATGCCTCGTTTTTTAGTAATGAAGAATTAAAAAATTCTCCAACAAGAAAAATTAATGCATTGGCTTCCGACCTATTTAAAAAAGTAAAACATAAACGTCCGATGCTTTCATTAAATAAAGCATATTCGTTAGATGAAATTCAAAAATTTATTGATAATATAAAGAAAATTACATCTGATTTTTCTTTTTTTATCGAGCCTAAAATTGATGGTCTTTCAATTTCTATTATTTACGAAGATGGTAAATTAGTTAGAGCAATAACAAGAGGAGATGGAATAACTGGCGAAGATGTTACGGCAAATGTTATTCAAATGTCTTGCATTCCTAAAACTATTAAATATAAGAAATCATTAGAGGTAAGAGGGGAAGTTTATTTAGCAATATCTGATTTTGAAAAATTAAATTTAAAATTAGGGCAAGAAAATAAAAATAAACTTGCAAATCCTAGAAATGCAGCAGCAGGCACATTAAGGCAATTAAATTCTCAAATTATTAAAGAACGCAATTTGTCAGCATTTTTGTATTATGTTGTGGACCCTTTGCAACATAATATTAAAACAATGCAAGAAGAACATGAGTTTTTAAAAACTTATGGTTTTCCTGTTACTCCTGAAGTTCAACACGCTAACACATTACAAGATATTGAAGAATATATTAGAAACTTCAAGAATCAGAAACTATTAATAGATTACGAAACAGATGGAGTTGTTATAAAGTTGAACGAAGTTCAATATTTTGATAAGTTAGGATCAACTGCTAAATTTCCTCATTATGCAATTGCATTTAAATATGAACCTAATGTTGAAACAACAACAATAAAAAGAATATATGCGACAGTAGGAAGAACAGGATTGGTTACGTACAATGCAGAATTAGAACCTGTAGAAATTTCTGGCAGTCTCGTAAGCTTTGCAACATTGAATAATTTTCAATATATAAAAGATTTGAATCTAAATGAAAATGATTTAGTATATATTAAAAAAGCTGGAGAGATTATTCCTTGTGTAATTGGCTTAATTTCTGGCAAAAAAGAAATTGATCATTTTCAAAAAGTAGAATTCTGCCCATATTGTCACAATAAACTTGTCGATTCAGAAACGGGATTGGAGCAATTTTGCTCAAATGATCAATGTCCTGAAATACAAAGAAGAAAATTGATTCATTTTTGTTCAAAGGATGGTTTAGATATTAATACTATGGGGGAGAGAAATATTGATCTATTTTTAAAAAATTCTTTAATTTCCAACCCTATTGATATTTTTAATTTAAAGAACAAAAAACAAGATTTATTAAAATTAAATAGAATGGGTGAAAAGTCTATTGAAAATATTTTGATTTCCATTGATGAATGTAAGAATAAACCTTTATATAAAATGATTTCTGCTCTTTCAATCCCACTAATTGGCGTAAAATTAGCTAAATTCGTATCCTCGAAAATTAAATCATTACATAATTTATTAAACTATGACTTTTCGCAATTAATTGATTATCATGAAATAGGAGATAAAATTATAAATTCTATAATTTCTTGAATATCTAATGATTCTAATAAAAAGTTAGTTGAGGACATTATTAATCTTGGATATGATTTAAAAGATGAAAATGAATCAATTACTAATTTATTGGAAAATAAATCATTTGTAATAACAGGAACATTGAGACACCCTAGGTCATATTTTGAAAGGTTAATTGAAAAAAATGGTGGTTTAATTCACTCAAGTATTTCATCTAAAACTAATTATTTATTAGCAGGTGAAAATGCCGGATCTAAATTGCAAAAGGCAAATAAATTAAATATTACTATAATAACTGAAGAAGATTTATTTAAAATGATTGGTCAATAAATAATATTCTCTTTTTATTTTAATATATGTATATATTGAAATAATATAAAAATTAATAGAATATCCTAATATTGAATTTTTTTCTACTTCAATAATCAAACAACCATAGCTTAAAGTATTTATTATTATTTCAAATATTGAAACATACATTTTTATTATAGGTTTAACTATAAAAGCAAATAAACAAAATACATAGTTAAATATTGAAATTGGTAGAAAGAATAATTGATAAAATATTCCTAAAATGTTAAATTTTGGATTAAATGAAATTACTATTGAAATAGAAGAAATATAAGGTAATATAATAAATAGAAAATTTACAAATATATTGTTTTTGTAAATTTTTTTAATGTCTAAAATTAAAAAAATAATCAATGTTATTGTAAAAGATAAAATAAAAGAATAAGAATACATGGTTCTAATATCAAAGAATATCAATAATAGTGCTGCAAAACTCAATATGTCAATGTTATTAAATTTGCCTTTAAAAATGTTTTTGCTTGCAATTTTTAATATCAAAAACAAAAACGCCCGACTTGCAGATATTGAAAAATTTAATAACCTTAGGTAAAAAAATAACATTAATATTGAAATCAAATCAACAACCAATTCCTTTGAATTCAATCATTTTAAAATTTTTGATATAAGCAAATATATTATTCCAAAATGAAATCCACTTATTGTAAAATAGTGCATAACACTTAGTTGTTTTAATTGATCTACAAACAAATCATTAGCTTGTTGAATGTTGAATAAAACCAGTGCTAGAAATTTTTTTGTTTTATAATCATAATCTTGCAATATTGGAATTTTTTCTTGATATGCATATGCTTTTGAAACCAATTTAACATTTAATAATTCAAAATCAATTTTATTGCTTAAACTAAACATATTTTGATTTTTAATTATTGATAAAGTGCCTGATAATTCGATATTATATTTGTAAAAATTATTGAATTCCCAACTGCTCCAATTTTTAACTAAGACATTATAATATTGATATTTAATTAATATTGATTTATTAAAAAATCTCTTTATTTCAAACTTACCACAAATATTCTTTCCATTTATTTTAAATAAATCCAAATATCACAAATAATGAACTAAAAATATTAAAGGAAATACTATTGCAATTTTTATTCAATATTTTAAATTAAAAAAATTCACTGCAATTATTAATGACAACATTATTGATGATATAACAATATTAATTTTATAAATAATTAAACAATATAAGCAAAAGCTTAGCGCTACAAATAAATTTGTATTTAAATTATTAAATGATTTTTTAATATAGTTAAAGTTTTTTCACCTACTCCTGGTAAATTTTCCAATTCCTGTCATGTTATTAAATAATCCTTAGCAATGAAGTAGCTTAAAATAATTTTTGCTATATCTTTTTTTATTCCTAAATCCTCTAATATGCCAACGTTATTTACTTCTCTAATAGAAATTGAATTATCTTTTTTAAAAGGAATATTAATTGATTGATTTTTTTCTAACTCTAAATTTAAATCAATATTGTTTAAATCTGCGCCCGCTTTAAGACGTGCTTGTTTTATTATTTCCAATAATTTAGTGCCTTTTTTAATTTCGTATGAGTCGGGATACTCTATTGCACCCGATATTCCTATTGTTATTATTTCATTTTTGTTTTTGATAAATAATTTATTGTTATTTTTTATATTTGAAATGTATTTATTAATTCCTATTGACATTAGTATTATTATTGATGCTGATGAAAATAATCCTAGTCCCAAAAAAATTTTTCGTTTATTCATTTATCCCTCCATCTTTATCTTAAATTAAAGGATTTTATTATTGATGAAAATGGGAAAAATTAGCGAAAAAACCGTTTTTGGTTTTATATAAAAAAATAAAAAACGCAAAGCGTTTTTTATTATTCTCAGATTGCGTTGTGTCAAACTTCTTGGACGTCTTCATCGTCTTCTAAAAAGTCAACAAATTGTTCAATTTGGCTTGCTTTTTCTTCATCTACTTCAATTTCGGTATTTGGGATATATGTAACTTCAGCTGTTGTATAATTTTCAATTTTGAATGTTTCGTCTAATGCTTGTTTTACTTTTTGAAATGCTGAAGGACTTGTAATTATTGTATAAGTTCCATCTTCATTTCTAAAATCTTCTGCTCCTGCATCTAAAACTGTCATCATGGTTTCTTCTTCACCAGAAACATTACTTGGAAATTCAATTAAACCTTTTTGATCAAAAACATATGGTATTATTCCTTGTTTTCCTAATTGACCATTGTGTTTTGAAAAGAAATGTTTGATATTCGAGCTTAAACGATTGAAATTATCTGTTAAACAACTTACAATAAAATTTACACCGCCAAATGCTGTACCTGAATATAAATAAGATTGATAGTTTGCAGCTTCTTTAGATCCTCCAGCTACTTTTGCAATTGCCTTTTCAATGTTTGCTTTAGGCATACTTTTTGCCTTAGCCTTAGCAATTGCTAATTTTAGCGATGGGTTAGAGTCTGGATCTGGTCCGCCTAATGTTGCAGCAACAATAATTTCTTTTGAAAATTTTTGAAACATTTGGCTTCTTTTAGCATCAACTGCAGCCTTGTGATTTTTTGTGGTAGCTCACTTTGAGTGTCCTGACATATTTTCTCCTAAATTTGATAGATAAATATAGGATATTATACCAAAAATAACACTCTTATTTTAGTTATTTTATATAATAAGAATATGTATTTAATAAGAGGAGAAGATAGTTATTTAATAAATCAAGAATTACAAAAAATTATTGATTTAAAATTTGAAAATCAAGACGATTATTCAGTAATTACTTACAATGAATCTATTGATTTAGAACAACTGTCAGATGATATATTTAACCAATCATTATTTTCTTTAAAAAAACAAGTCATAGTTTTAAAAAATATTGATTTTTTTAATTCTAAGAAAAAAAATGACAATAAAAAAATGAACGATTTTTTACAATTGCTTGAAAAAGGGCAAGATAACAATTTAATATTTATTTTGCAAGATATTAAAAAAAACGATATTTCTTTTAATCCTTCGTTGGCTTATAAAAAAATTGAATATATTTCAAAAATAATTGAAATTAAAAAACCAAGTGATAAGGATGTATATAACTATATAAAGGAATATATTTTAAAAAATGGAGGCAATATATCTCCAACTACATTACTTGATTTTTTGTCAATGTTTCCTAATGATTTACAACAAATTATTAATGAAGTAGATAAATTGCTATTGCAAAATAAAAATATTGATATGTTGTTGATCAATAATTCAAATATTATTTCTTCTGACAACATTGAATTTAATTTATCAAATTGTATTTTAAAACAAAAAAGCATTGATATTATAGAAGCAGTAGATGAGCAAAAGAAATATGGTAATGGTGCTAGTGAAATTCTAAGCCAAATAAGTTATTTGTTTTATCAAATTTATTGCACGTATTTGTTGATGGAAAATACCAGTGATAATGAATATATATCAAAAACTTTATCAATACATTCATATAGAATCAAGTTGTTTATGTCGTATATAAAAAAAACAAACAAGCATAAATTAAAAGAATTAATAATGTTACTTGCTGATATAGATATAGCAATTAAATCAGGATTATTAGATGAAGAAAATGCCTTAAATAAATTTATTTTATTTTACATAGAAATATAAAGTTGGTGTTATGAAAAAAATTTCTATTTATGAACTTAATTTAAGCACTTTTAATAATAAAAAGGGTTTAAACAAGTATTTGTCAATTATTGAAAAAATTGAATATTTAAAATCATTAAATATTGATATCATAGCAATTGATGATATATTATTGAAATGTTATGAAATCAATAGAAATTATGAATTTAATGATTGATCGGATTTGCAAGATTTTAAAAAATTAGTTGAACTTTGTTTAGCAAATAACATTAAAATAATGCCAACAATCAATATTGCAAAATTAAAAAAAAGTATTATTGCAAAAAAACATTTAATTTCTATTTATCGAAATAAAAGCAGCGAAAATGAATCAAATAAAAATGATATTGAATCTTATTTATTAACCCAAGAATTTTTAGTTCCCACCATTGAAAATATTTCAAATTTAAGCGTTTTTTTAAAAGAAATTATTAGTTTTTATAGTGTATTAGGCATTAAGGGATTTATTCTTGAAGATTTTGAATTCTTAATTAATAAAAAAACAAATAACATGAAGTCAAAATTTAGTTTTTTATTAGATATATATAGAATTATCAAACACATAAATTTTGGTTCTATGGTTATTTGAAAAACAAAGCAATATAATGATTTTTTAAACATAATTGATATTGCTAATCAAAACGATTTAATATTTTTTGATTATATTTATTTAACTCATTTATCTGACATAAATTTAAAAAATTCATGAGATTGAATTAAATTCAATAAACTAAATGTAAAAAAATTATTTGAATTGTGAAAACCTTTTTGCAATAATGAGCAATATATACTTTCTTTATCATCAAATTATAGCGGCTGAGTTGTAAACAAATACGGAGATAATTTATCATTTTATAAAGAGAGTGCAAAATCTCTATTAATGTTTTTAATGCTTGCAAAAAATTCTTATAGTTTATACAATGGCGATGAATTAGGAATTCTTGGTACTGATTCAAAGAATATTTTTAATTTCAATAACATTAATTTTAATGAAGAAAAAAGATATTATCAATCTAGGAATATTTCTAGTCAAAAATATATTGAGTCTCAAATTAAATTCAATCCAATAACACTACAAATAGAATTTCCATGGACTAATAACATTCAAAATTTAGATTTTATACAGCCGAAATTGCGTAACAAAATTAATGTTGAACAACAGTTGACCAGTAAGGATAGCACATTTAATTTTTATTCAAATTTAGTTAGAATTTTAAACAAATCAAAATATTCAATTTATTTTAAGGCTTTTCCAAAAGTTTTACTTTGCAATAATTTAATAAGTATTGAATATAGTACTAGCGAAAATAAAAAACTTTTAATTTTATTAAACTTGAATTCGTTTCCAATAAAATCATATATATGAAATCAATATCTAATTCTTTCAAGTTCTTATACAAATAAATACTATTCAAGTATTCCAGAATATTTAAGTCCATATGAATGTTTAATTTTAATTAAAGATAATAATTAATTAGTTTATAATTTTTAATATATTTTATTTATTTAATAATAAAGGAGTCTTATGCAACTACAATTCGATGCATCACAAGTAGCAAAGGAACAAAAGGCGTCAACAGTTGGTTCTGCAATTGTTTATACACTTTTAGCTATAGTTTTGGCTGCCGTCTTAGCTTTTTTAATTTGAAAATTAATTAAAAATAAAGTTTTAAAGAAGAGGGCTCAAAAAGTTGAAATGCAAAAGCAAAAAGAAACGTTAGCACTATTTTATCAATATATTTTAAGCTTTTATGAAGTTATAAAATTTACAAACCAAGAACTTAAAAATTTTGAAGTTTCAATAAGCACTCATCCAATGGGTGAAATTAAAGAAGGCGCTAAAAGGTTACTATATCGTTTAATTACAAGAGATGATTTTTCATATTCATTTGTTAAAAATGAACAATACAAAACTTTTGTAAAACACGCCGAATTAATAAATATTACAAATTGCAACTTATGAGATAAAAAAATACCTGAAACAATCAATTATTTTAAAGAACAATATGAATTAGTCCCTTTAGGAAAAACAAGAGATGACTATATACAATTAGTTCAAAAATCAATATCTGAAAGATTTTACAATGAAAAATAAAAGTCAAAAGAAACAAAAGATAATAGTCTCTAAATTTGGTGTTGCAAATTGATTAACAGTTTTAAGAATGTTGCTAATGATTCCTTTTATTGTTTTTCTTTCAACTTCATTTGGTTTAATTTTAAATGGTGTTCAACTTTCTTACAAAACCAACAAAGGAATAGTGCTATCCATTTTATATTGAATAAATGTTGCAATTTTTATCATTGCAATGATAACTGATTTTTTAGATGGATTTTATGCAAGGAAAACAAAAACCGTATCCTCATTTGGAAAGATATTTGACCCAATTGCTGATAAAATTGCAACTACATTGATGTTACTATTCTTAGCGACATATAATTTTGTTTATATACCTATTGCAATTTTGTTTATTATAAGAGACATAATTGTTGATGGTACTAGAGTATATGCTGTAAAGAAAAACATTGAAGTATCAGCAAATTGATTTGGCAAAATTAAAACTATTTTAGTTTCATTAGCGTTAGTAGTGATTTCAATAGGAGTTCCTTTTATATCAGATAAATCAGATTATAAATATATACTATTTTATTTCAATATACCTTTAATAATAGGACTATTATTAGCGTGAATTAGTGGCATTATCTATATTATTAAATATACAAAAGGAATTAATAAAGAATTGCTTCAATCATATACAAAAAATGATTCAGATGCAACAAATAAAAATAATCAAGATCAAAATAATAATAAATCAAATCAAGATTCTAGCCCATCGCCTAATTGCTAATTTCAAATAAAATTTAAAATGTAACTCAATTGTTACATTTTTTATATTCTTTTCAATAAAATCTCTCAATTATTATTGATTAAATTCATAATTTAATCAAAATACGTTTAAAAAATTTATAATTCTAAATATATTTTAAATGTGAGGTACTTGCAATGAAATCAAAGAAAATAAAGTTATTAAAGTTATTATTAACATCGTCAGTTGCAATTTCGTGTGCAATATTACCTGTTTCTTGTTCTTTATTATCTAATGATAATGATAAAAAAATAACAATAAAGAATTATGAATTTAATAATTTATCAAGTGAATACACAATTGAATCTAAAAAATTAAATTTATCGTTTGTTGATGATGGCAATGTTCCATATGTCAATTTACTACAAATGATAAATTCTTTAGATGGATTTTTTGATAAAAACTATATAAATTTTTCGACAAATATTTTATTCTCCGAATTAAAAATGACAACTAAATTCGGATATATTTCGTTTAACTGAAAAACAAATAAAATTACTATGCCAAACACTTATTATGAATATATAACAAAAAGCCCAGGAAAGACTAATTTCAACAAATATTTGAAATCCAAATCATATTATTCAAAGGATTTTAATAAAAATACAATTGTTATAGATTTAAAAAAATATAATTTTGACATTTTATATCATAACGGTAATGTTTTGCTACCATTAGCAATATTTAATTTGTTATTTTGCTCAACTAATTATTACAATTTGTATTTCAATGGTAACAAAATCTATGGGTGCGATTTTGACATCAAATCTAATGTGCAAGAAATTCCTCAAATTAGAAATAGCAAATTTCAAAACTCACTTATTCCCAAGGATATAGTTGAAGCAAAATTTAACTTCACTGCTTTACTAATTGATTATTTTTACGGCCTTAAAGAATATAATAATATTAATAGTTTTTATTCTACAATTTCAAATGAACAAAAGCAAAGAATGCTTAGTCAAAATCCAGTTATTTCAACAAAGGAATATATAAAATATTTATACAATTTAAATGATTTACACACCTGAGTTTCAATGCCTTCATTTTATAATCACAGAGATTCTAGCATTGCAAAAGATTTCTATGAATATACTACAAATAAAAGAGAAAGAATTTATCAATCTACACGAAAATATCTAAAAAATAATAAATTAAATAATTGAGTAATTCAAACAAGCAAAGAAAACAGAGAATTATTTGAGAAATACGGTGTTAGATTCATTTCAAACAATACCGCAGTAATTTCATTTAACTCATTTAAAACCTTTCAAAATTTAAATGATAATTCTACTAATAAAGGATTTAGCGAAGACACATATTTAGGGTTTGCTAATGCTATGAAATTAATCAAGAAAAACCTAAGAAATATGAAAATAAAAAATATTATTGTAGATTTATCAACTAATAGTGGCGGCAATATTGGCGCTATGCAAAGGGGATTGGGTTTTATTACAGATGAACCAATATTAAACAAAACATATAATCGTTTAGATGAAGAAATAAATATTAGATCTGTTGAAGTTGATACAGATGGCGATGGAGATTACAAAAACGATGCCTATTCTGAATACAATTGATATCTTTTAACCGGAGTAAACACTTTTAGTGCTGCAAATACCTTTACTTCAATTTTTAAAGAAATGAAATTGGGCAAGATAATAGGACAAAAAACAGGTGGTGGTGCAGCATCAATTTTACCTGTTGTTTTGCCAGATGGAACTTCAATAGTAATAAGTTCTAATGATTTATCCGTAACTATGAAAAATAATACAATTAAATCTATTGAAGGTGGAATAGAACCTGATATTTACTTAGAATACAAAGATTTTTATAATGATCAAAAACTATTAGAAATAATAAAAAATAAATAAATCAATACATATCATCATTTTTAAGTAGATTGAAATTCATATTGTTCAATGTTTAAAAATATTGCAACAAAAACAAGATAAGAGTTTTTAAACAATTATCTTGTTTTTTTATATAAGAATATATGATAAAAAAATAACTAAAATAGAAATGTATATTTTATGGTAAATAAATGTCTCAAATTAATTAACCTTGTTGAATTTTTAAAACATCAAACCTACCTAAAACATTAGGTCAGTCAATTACCAAACTTTAATTTTTTCTAATTTTTTAAAAAATTAAAAATAGAATTTTTAACAGGCTATTGCAATTTGGAAATGCTTTTTTAGCTACAACGCATTTTTGGTAATATAAAAATGAATTTCATCAATATAAACAATTAGATAATAGTTAAAATAAAAAACCTTTCATAATAATTTTATCTACTAAGAAAGGTTTTTATAATTACTAATTTTTAATAATTAATGTTAACAATAGTTTTTATTTAGCACTTTTTTTAGCATTATTTATTGCTTTAAAAACTTTTGGTAGTTGAGATGTTAAAACGTTTTTTAAATCTTTACTATAAATATTTGTGATTAGTTGCTTAAATATCAATATTATTCCTTTAAATTCATTAAAAATAATTTCATATTCTTGTTTTGTTATTTTATTTGAATTTACCATATCATTTAATTTCTTTTTTAAATCATTGCCGGATGGATCAGAATATTTTGGCTCTATAAATTTTTCTACATCAAATAATATAGGTCATCCACCAAATAATATCAATACTTTCTTTCCTCCACCTTTATTATCATATTCTGTTTGTTTTGGACCATCATTGTGGAATAAATAATCAAATTTAAGGTTGCAAAAGTAATCCAAAATATATTCTAAATGTTTTATTAACATGTTTTTTAATTCATTATATTTGCTATCATTTGTTTTTAAAAATTCATGTTTTTCTTTAAAGCTATTCAATATTGGAGTTAAATATTCAATAAAAACATCAGTTCCATTTTTAAATTCTTTGAACATTTTTATAGCTCAGGCTCCAACTGTCTTTGATAAAACTCAGTCTGCTGTCAGATTTTTATAATACATAAAACCCCTACCATTAGAAACTTTATCATCAATGCCTAATTCTAATAATACATTTAACATTTCATCATTGGTGTTAATAATTAAATTAATTAAGATGTTGTAAAGTTTTTTAATAGATTCTTTGTTAGAATTAAATTGACCAAATACATTAATTGCTTTATTTGCTAGTTCAAATAACGTTTTTTCTATTTCTTCGTTAAACTTTTTATATTGTTCAATTATTGCTTTAATTACGTTTGCATGAGCGGCTTGTTTTCCTTGTTCTTTTCTTGCTTCATTAATCTTTTTTTCAATATTTGTTTCAATATTTAATTTAACATTTTCTAGATATTTTTTAAATTCATTTGATTTTTTTACATCCGATTCTAAATTTTTAGTTATTTCGCTTATATCCTTAGCAAATCCATAAAATTCAAATGTTCTTGATTTAGTAGATTCTATTGGATTTTTACTATATTCATTCAATAAACTTTTATTTGACTTTAACTTAATTTTATAAGTTAATTTTCCCAATGTTTTATCATATTTAACCGCCTCTATTTCATAGATAGCTGCAAAATCACTATCTTTTAGATTAAATCTATAATCTTGAATTGTAATATCTTTAACATCAATAGATTGCTTTTCAAAATCATTATTTTTAGTGAATTCCATTGTAAGTTTATCCAATAAATCGTCAAGCCTTTTTGGTTCATTTTCTAATTCTTGTTTTATTTTTTCAATGCTTGCTTTGTCATTGAAACCTGATTTAGTAAAGGTTTTTATTTCACTATAATCATTTAGCTTAAACGTTATTTGTATATTGATAGTATCTTCTTTTTCACTTGCCAATAGTTTATAATCTTTTTGAATTTTATAATCATCAATAATATAAGATTCTTTTCCACTACCAGAATCAATTTTGTCTTTAAAAAAGAAATTGTAGCCTTTATTTATCGATTCATCTATTGTCATTAATTTTGCTAATTCTTTTTTGTTGTCATTTTTAAAATCAAAATCAAACTTATTTGCAAAATTTTTTAAATTTCGTTTATTCATAATATTAGCAATAGAAATTTCACCCGAGAATTTTTTAAAAAGTTTATCATTTGCCGATTTTATCTTTAAAATAAATTCATATTTTACAGTAGTATTTGCTCATTTTTTTAATGACACAATAGCGTTTGTGCTTTCTTGATTCAATTCGATAATTGAATTTTGATTTAGAAAATCAGAAATACCTAACAACTTAGCAATCTCTTGTTTATTATTGGCAATTTTATTGTTAAAATCCGCTTCAGTATAAACACCATCAGTTACATAAGTTGATTCTTTTGAAATGCTTAAGTTATCAATTAAATTAAATGTGTAATTTTCATCACTTAAGAAATTGAATGTAACTGTCTTTTTGTTTGATTCTTTTGTTCCATGTTTAAATGTTTTATTCAATAACGAAAAGGTTATTTTTAATTTTCCATTGGCATTGGCCTTGATTTCTGAAATATTTAATTCCAACCCTGCTAGTTCATTACTTTTTTGCTTGTTTAATTCAAAGTCATTTTCTGAAATCATTGAAGATTTTTTAGATGTTAAATATTCATTATTTTTTAAAGATATAATGTTTTCTTGTTCAACTAATGTATCCAATCTTTTTTGTTCAATATTGATTTCATTTTGCTTTTTAGTTAAGAAATTCGTAATTTTTTTTGAAATTTTTGATTCAGAAACAATAAAGGTTTTAAATTTTTCTTCTTTAACTTTTATTGTTCAACTTACTGACAAAATTCCTTGATCATCATCTGCAATAATTGATGGATTTCCTTCAATAGTATATTTTGAATCGCTTATATTTAGATGAATTTCTTTATCTTCAACATTCGAAGGTAATATATTTGACTTATCATTTTCTTTATCAAAAACTAGATTTGCTTCTATCTTATCATCGCTAATTAATTTATTCAACTCTTGCTTACAAGCTTTTAAGTATTGTTCTTTTGTATAAAAACCTGACAATGTTATAGTTTGCTCATTTGATTTTAAATCTTTATATTGTTTTTCGTTTGTTTTAACAACAAATTTAATTTTTAAGTTTCCTTTATCATCATTTGATTCACTTATTTCTAAAATTTCAATATAAAAATCATTGCTAATTTCTGTTTCAAAATCGTTTATTTGAACATCGGAAGCAAATGTTTTGGCAATATTTTTTGAAGAAATTAATCTTAACATTGTTGTTTCTTTAGCAACTTTATTTAAAGACTCTATTTTTTCATTTTTATCCATTGTTTTAGTTTTATAACCACTTAATTCAACTTCAATAAGTTTGCTTGATTCGCAATATTCATTAATTTGTTTGTTCAATTGTTTGATTGCTAATCTTATACTTAATTTTCCACTTTCATCGTTTGGAATTAATTGCTTTACAACAACTTCAATATTTTTAAAATTATTATTTGGCTTTGGAATTTCAATATTTAAATCTGATTGCTTGATTGAAGATGCAAATCTTTTAATAGTAGTTTCAGGTTTCTTATCGCTTACTTTTATATTTAAATTTCCTATTAATTCATTTAAATAAGCAATTGTTTCATTTCTTTGGTTATTAATAAATTGTGCATTTGTTAAGAAATCTGTCAATTTTACTGTTTTAATTTCTGTTTTTAAATCGCTGAATTTTGGGTTAGAACTTTTTAAATAAAAACTAATTAACAATGTTCCTTCTTCGTCATTGGCTTGCATTTTTTCAATAACTAACATTGCTCCATCAATATTTTTTTTATTGAAATCAATATTTTTGATTTCTGAAGGTTTTTTGTTATGCTTTTCAGGTGCCAAAAATTCGTTCAGATCAATTGAATTAACTAAATTAGAAAGTTTCTTTGATGTTTCTTGTCTTTCTATGTTGAGTAATGTATTAAATCCCACAAATTTTTTAGTAACAATATTTGATTTTAGATTCTTTTTATTGTCATTGTATCAATAGCTAATAGTTAATTCACCTTTTTGATCATCATAACTAAATTTTTTTTGCAATTCATATTCATTATTTAGATTATCGAATTTGAAATTGCTTAAATTTTGAATATATTGAGATGGCAATATTGTATTTATATTTTTTTCATTTATTGATAAATTAACTTTATCAACCGCGATATTAAGATTTTTTAAAAATGATTTAAAGCCCGTAATTTTAGTTGTATAAACCTTTGATTTAATTCAATATGAGGCGCCACTTTTTTTATCAATATATTGTTTTTCAAAATAATAATTTAATTTAACTTCCCCATTTATATCATCAAAAATTAATTCACCATTGCTTCGTAAATAAGCTGAATTTAGTTTTTTAGATGTTTCAAAATCACTTCAATTCAATGTTGAAGCATATGTTTCTGATTTATTAATTTGTTTCTTTAATTTTAAAGATATCTCATTTCCTAACTTATTTAAATCGTTAGCTTCTTTTTGCATATTAACGATATTATTCAATGAATTAAAACCATAAAACGTTTTTGAAATAATTTTTGATTTTATATCTTTGTCTTTTTGTACATAATAATATTTAACTTCTATTTTGCCTTGTTCATCGTTTGCAATATATTCAGTTTTTAATTCATATTGGGAATCGATGTTTTTAAATGAAAAATTGCTTTGCAATTTAATATATTGCGAAGGCAAAAAGTCTTTTTTATTAAATTCATTTATTTCTAGATTAACTTGTTCAATTGACGAATTTAAAGGGGCAACCAACGCTTTGAATCCTGTGTATTTTTGAGAAAACATATTTGATTTTATTAACAGTGGTTGTCTTGTTTTAGAATCGATTATTGATTTTGTAAAATAATAATTTATAATAACATTCCCGTTTACATCATCTGCAATAATATTAGAGATTTCAAACGATATTTCTTTATCAAGATTATCGACATAAAAATTTGATGAATTAATTTCACTTGGAAGTATGTTATCTTGTTTAATAGAAAGTTTTAAATTTGGTTTAATATTTCTTGTTTGTTCTTTTAATTTTGTTGTTTCAGTTTCTTTATCTTGCGTCTTGTTGCAGGCTGATGAAATGACTCCTGATACAAGTAAAATGGCAGTAGCACTTAAAATGGAAATGCTTTTGATTTTTGTATTCTTTTTCATATCTATTTTTAATATTTCTTTCCTTTTTAATTAGATAAAAAAAGCATAAATGGAAGACTCCCATTTATGCTAAAAGTAAAAATTATTTTAAGATTTCTAACACCTTTGGTAAATCTCTCAATGATTGTTCAAATAAGTTAATTGAGTTTTTTAGACCTTCATTTAAAACGTTGTAAATTGGTCTAATTACGTCTTTTACTTTTTTAATATCTTCTTCAGACATTTTTACAGATTTTAATACCGCAACTGCTTCTGCCTTAGCGTCAGATAATAATGTAATTAATTTTTCAAATGTTTTTACATATCCTTCTTCAGCCAATTTGCTAAAATCAAATTCTTTTAATTTCTTAGCGGTTGTTTCAAATAAATTATCTTTTCCAGCAAATTTATTGTCAACAGCTGTAATTAAATCGTTTAATTGAGCTATTGCTTTTGTTGATTCGTTTGTTTTTAGTGTTTCAAAAATTACTTTTGCATATTTAGAAATATTTTTTCCAAATGCTACCAAGAAATCTGCCAATCCGTTTTCTTTTTTAATTAAATCAGAAATTTTTTCTTTTGTTAAATAATTTTTAATTGTCATTAATCCATCAGAAACTTTTGTTTTATTTGTGTTGTATTCTTTTAATGTTGACAAAATAATATCATCTAAAATTTCATTTAATTTAGAAATTGCTTCATTATATGTTTTTGTAATATTTGGCAATTCTTTTTCAGAAACTGCAGCTTTAATTTCTTTATTTTCAATAATTGGCTTTAAAATGGCTTCTGATTTAGTTTTTAATTCTTCGCGATATTTCTTTAATTCACTTTCAAAATTTGCTTTAACTGTAGCATCTGCAATACCATCAGTTGCTTTTTGAAGTTCGTTTTTTTCTTCTGGTTTAACTTCAGGTGTTGTTCCTGGTTTTTCACCTTCGGGATTCTTTTCGGGTGTTGTTCCTGGTTTTTCACCTTCAGGATTCTTTTGATCTTCTGGTTTAACTTCTGGTTTAGTTTTTACGCAACCTGCAGCAACCAATGGAGTTGAGGCTAATAATGGAAGTGCTACCCCCATAGTTAATAAAATTTTTTTGGATTTTTTCATTTTATAATTTCCTTTCTATATAAAAATAAAATATATTAACTTCATATAATTTACCATATGGATTGTTTAAGAACAATTTAATAAATTGGATTTCTGAAAAAAAAAAAAAAAAATGAACCAAAAAGTAATTTTTTACTGTTAATTATTAAATTACTTTTTAATTTAAGATGCTTTTTATAAGTAAGAAAAAAATTTTATTATTTCTAAATTTAATGAATAGCTATGCTTTAAAGATTCGTAATATTTAAAATATTAAAAAATGTCTATATAAAAATTAATTTTTTTTAATTTAACTTATAATTTATATTAATTATTTATATATAAATATTTTAAAACACTATATTTTATAGGAGGACACTATGCCAAGAGAAGGTTTAACCTTAAGATGTGAAAAATGTAAAATGGAAAATTACATTACAAAAAAGAACAAAAAATTACATCCAGACAAAATGGAAGTACAAAAATACTGCTCAAAATGTAATGCACATACAACACACAAAGAAAAGAAATAAATTTCTTTATTAGCATTAAAGCTATTAATATAAATAAGGATTTAACATGATTAGAGAAAAATTAAACAAAATTTTAAAAGATAACGAAATTGATGCAATTATTTCAGAAGCTCCTCAAACTCGTTTGTGATATGCCGGAGTTCAAACAAGCGATGGATTTCTTGTTATTGAAAAGGAAAAGGCATATTTATTTGTTGATGGAAGATATATTGAATATGTAACCAAGAATTCAAAAAATGTAGAAGTTGTATTATTAAAAGGCACTTCATTTAAAGATTTTTTGGCTAAAAAGAAATATGCAACTGTTGGTGTTGAAAAGGACTATTTAATACTCGAAACATTTGAAAATTTAAAATCAATGTTGCCAAAATCTAAATTTGTGTTAGTAAATGGACAAGAATTTAGAATACTAAAAGATGAAGAAGAATATAGCAATGTTCAAGAAGCATGTAACATTTCTCTTCAATCTTTTGAACAAATAAAAACCGTTCTAACTGAAGGAATTACAGAATTAGAAGTTTCAAATAAATTAGGCTACTTAATGCGTTTATTTGGAGCTGAAAAAGAATCATTTGAAACAATTGTAGCTTTTGGTACAAATGCTGCTGAACCTCACCACCATCCAACAGATCGTAAACTTGCTGATGGTGATATTGTTAAGATAGACTTTGGTGCTCAATATCAAGGTTGAGCAAGTGATATCACTAGAACATTTTTCTTTGGAAAACCAAAGAATCCAAAACTTGTTGAAATCCTAGATATTGTTAAAGAAGCTCAAAAATTAGGAAGAGAAGCCGTAAAACCAGGTATTGCTACAATAGAAATTGACAAAATTTGTAGAGATTATATTGAAAGCAAAGGATATGGTCAATACTTCACACACTCAACAGGACATGGAGTTGGAATCGATGTTCATGAACTACCTGGAGTAGGAAGAAATCGTGGAGATGCAATATTAGAAGAAGGTATGATTATTACTGTTGAACCTGGTATATATATCGAAAACGAAGGTGGCGCTAGAATAGAAGACACTATTTTGGTTACAAAAGACGGCTCAAAAGTATTAAGCAGACCCGAAGATTATGAAAAATAATTAAATAAAAAAATGAACAGCTGGAAAATCCGGCTGTTTTTTTCGCTATATTTTCTTTATATTTAAATATTTCTAATTATCAATGATAAAGTTAAATCAAGGAGAATAAATTATATGAACAAAGAAGAATATTTAGCTTATTTAAGTTATATAAATAATGGCAAATGACACCAAATCTATAATGATTTAAAATCACAAATAACATGTCAAAAAACAAAATATGCCGATTTTTGTAGTGACCAAGCAATCAAAAACAAATCATACATAACAATTTTTGATAGTAATTATCCGCTATATTGCAATAATGTTTGAAATCCACCGTTTGTCCTTTATTATTATGGAAATAAACAACTATTAAAGCATAAAATTATTATGATAACTGGAAATATTTCTATTTCTAATATTATTGAGGTTAAAGATTCTATTGAAAATTTTAGCAAAGAATATGTCTTCTGCAATGAAGCATGAAAAGGTTTAGACATACAATTAATTGACTATTTAATTTCAAAAAATAAAAATATTATTATTATTTTAGCATGTGGAATTGAATACGCTAAAAATTCTTTACCTAAGAAATGATTCGATTATAGCAATTTGCTATTCATTTCTGAATACCCTAGCAATTATCACACAAGTAAAATAACGCTAAAAGCGCGAAATAGAATTAGCGCAGCAATTTCTAATTATTTAGTCTTACTCTCTTGTTATAAAAGCACCATGAATAACCTAATAGATCAATTCTTAAATATTGGCAAAGAGGTTTATTGCCTTGATGATATTCAAAATAATTTGAATGAAGATAATTTCAACAAAAACTTAATTAATGAAGGAGCGAGAAAAATAACTTCAATAACTGATTTAATATTAAAATAGAATATTATCTTTTTTATTATCCTCTAAAAGCATTATTATTTCATCTTTGTAAGGACTTTTATTATTAATAAACGGTGTTTCTAAAATAATAGGAATATTATCGAAGTCTTTATCATGAACAAATTTTTTCAATGTTTCTAAACCTATATGGCCTTTATCAATGTTTGCATGACGATCTTTTTTTGATCCAAGTTCATTCATAGAATCATTTAAATGAATAACTCTAATTAAAGGCAATATATTTTGTTGTTTTATATAAGATTTGAAATTTTCATAATCTTTTATATCATACCCAGCATCTCAGGCATGGCAAGTATCAAAACATATTGCAATTCTTTCGGAATTAACGGATTTTATCACGTATAATAAGTCATTGAAATTGCTGCAAATTTGACTGCCTTTACCTGACATTATTTCAAGACATATTATTACGTCCTTAGTAGATTCAATTATCTTTTTAAGAGATTGAATTAATGATGCTAATGCTTCGCTTCTGCTATATTCAACGGATGAACCAGGGTGTAAAACTAACAATTTAGCACCAAAACTATTCATTCTTTTAATTTCACTACATAAAAATTTAATCCCAAAATCTGCCTTTTCTCTATTGGCTAAATTTACTATATATGGGGCATGAACCACAATATCAGATACAGGAATAATACTATTGCATTGCTTTAAATATTTTTCAATATTATACAATGAACTATCAACTCTAAAAGCACTTTGTGGTGCTCCTAAGTAAATCATAAAACAATTCTCGCCATTATTAATAGCTTCATTAAATGAACCTATTAAATAATCGGGTTTTTTAAATGATATATGGCTTCCTAATTTAATCATAAACACTCCTAAATTTATAAAATTGTCTAATCTATTAATATTTAATTATATTAAATTATAAAGTTTAAATGCTCTTTTTAAATCATTCATAAATTTAGAAATAAAAAAATAAGGCTACATGTAATTTTTGATTTATAAACAGCCTTATTAATTGTATGTGTTTGTTTTAGTCTAGATCATAGCTATCGCTATTAAAGAATTCCTTCAATTTTCTACGTTTTTGAGGATGCTTCAATTTACGCAAAACTTTTGTTTCTAATTGTCTAACTTTTTCTTTCGAAATGCCTAATTCTTTTGAAAGTTCATCTAAAGTATGAGGTTTGTATTTGTTTCCCTTTAAGTCGTTAATTCCAAAACGTTTTCTAATTAAATCCCGATCAGCTTGATCACTTAATCCTTCTTCAATCATTTCATTTATAATAACACTTAACTCTTCATGACTTGCATACGAAGTTGGACTAATTACGCTTTCGTCTTTAACAAAATCTGAAAAACTAGAATTTTCTTCTTTTCCAATGTTTTTATCCAAACTAATTGGATCAATATTAATTTTTCTAATATATCTTACTTTTTCAGCAGTAAAATCTCCACCATATTTTTTAGCAATTTCTTCATCTGTTGGAGGATATCCATTTTCTTGTTGCAATTCACGTTCAATCTTCAATATTTTATTTATTGTTTCAACCATATGAACTGGCACTCTCACAGTTCTTGCTTGATCGGCTACTGCTCGTGTTATTGCTTGTCTAATTCATCAAGTAGCATATGTTGAAAATTTAAATCCTTTTGTGTAATCATATTTTGAAACAGCCTTCATTATTCCGGCATTTCCTTCTGATATTAAATCAATAAAATTCAATCCACGATTTTTATAACGTTTTGCATTATTGATTACTAAACGAAGATTTCTTTTAACTAACATATCTCTAGCTTTTTTATATTTATAATAATTATTTGCTTCTTTAGCTTCTTGCATTTTTTTTGCTAGCGCTTGTTCTTCATTTGTAGTTAGTAACTTACCATATTTACCAATTCATCTCATATATCATTTAACAATATCATTGGTTTCAGTTAGTTTATTAGTTAATTTTATATCATCTTGATCATAAACTTTTTCTTCACTCAACTTTATATCTTCATCATTGTATTCTTTTAGCAAATTGTTAGCATCAAAGTCATCGTATGATTGTTTTTTACCTTGCTTGTGGTTTGTTTGATAATCGTCATCTTCATATTCGTCAAAATCTTCGCCGTAATAATCATTATATTCTTCATCATCAAGATCATTAAATGCCTTTTTAACAAAATGATTATCATCTTCGTCAAAAGACATTTCTGAGTCATCTTCTTCCTTGCTTCTAATTGAACTATCGTCAAATTCTTGATCAATATTTATATCTTCGTCAAGATTTTCTTCTTCCAACTCTTTAATTTTTGAAGGATCAAAATCTTTTTCTTCCTCTTCTGCGTCATCGTCATCATTATCATCATCTTTAGTTGTTGCGTGAGCAACTTCATCATCTAACGAATTTATGTCAATATCTTCAATATCTTCGTCATCAGTATCAGATGAGACTAGATTAGCTGCAAAAAGGCTTTCAAGTAAAGCTTCTGCTTCTTCGTCATCAACGTAAAGGTTCTTTTTGTTTAGGAATTCAAAAATTTCATCTTGAGTAAAAGCCTTTTTATCCTTATTTTTCTTTTGGACTTTTTTTAACTCGTCTGCAACAATTTTAGTTATACACTTAACTGATGATTCTACATTATTTTTAGTATCTTGCATAATTATTTCCTTCTGTCCAAATTGATTATTAGGTCTTTGGATTGGCCTGTTTCTTTCATTCAATTATTTAAATTTTTATCTAGTTCTTCTTTTGTATATTCTTTGTTTTTAAGACTCATAATTTCCTTGAATAAATCTAAATATATTTGTTTAGCATTATCTTCAAGATCAATATTGTTTGTAATATATGATTCAAAATTTGTTTCATCTATTGATTTATTATTTTTTATGCTGTTTATTAAAAAACACAAAAACATTTTATGATTTTGACATCCATTAGAAAATGCCATTCTAGCAAAATCAACAAGTTGATAGTTAGCAATGTATTGTGGGTGCTTTATAATGGTTTTTATTATTAAATAAAATACTAAATTTCTACCAGTTATTAATTTAGGCTGTTGTATTAAAGGTTCTTCAAAAAAATCAAGTCTATCAAACGAGGGATCAGAATATGCAAAATTAAGATCTTCGTATTTTTTGTAATTTGAGTTTTTAGAATAATTTTTTTTATTATATTCATTATCTAGACTAGGTTTTAAATTATTTTTTGAAATAGGATGAAGTGGAAAGTTTTCTTTACCATATAAGAATAAATATTTGTTTCAAGTTTCAACTGAAAAAATTTTATTTTCAATCAATTTTTCTTTTAATTTCAATATTAATGGTTCTTGCAAATAAATCACAAATTCAAACAATTCAGCATTTAATTCAACCTTTCTTGATTCGTTGGTTTCTAATTGAAATACTTGTTTAAATTCATTGAATAAATACATAAATAAATCAACAGGACAACATGCTATCTCAGACAATGTTTTTCCTTCGTCAATATTATATAATTCGTCAGGATCTTTGCTATGTTCATTCAAAACAAAAGAAACTTTTAAATTATATCTTTTTTGATTTGCTAATATTATTCTTAAATTTTTCTTAGTAGCATTTTTGCCGGCAAAATCATTATCAAAAAACAAAACAATATTACAATTTCTTAAAGATTCTAAATTTTGTATAGATAAAGCTGTGCCTAAAAGTGCCACTGCATTTTTTATGTTTATTTTATAAAGAGCAATGCAATCAAATTGCCCCTCAACTAAATAAACACTTGATTTCTTTATAATTTCATCCAATGCATGATAGTAATTAAACATAACTTCATGCTTTTTAAAAACTATTGTTTCAGAACTATTTAAATATTTTGGTTCAACATTATTATAAATACTTCTTCCAGAAAAAGCAACAATTCTTCCATCCTTATTTTTGATTGGAAACATCAATCTATCATTAAAAAAGTTATCAAAATTGTTGCTTGCTATCAATGATGAATTGGCTAAATTAAATGATGAACAATTTTTAGCAATTAGCATATTACATAAATTTTTATCTTTATCAGCAAAGCCAATTTCAAATTCCTTAATAAGTGGTTTTGTTAACCTTCTTTTTTTAAGAAATAATTTTAATTGCTCATTTTGATTAATTTCTAAAATTTCAAATTGAAATATATCGCTCGCTATTTGATTGATATTGTATAAATCTTCTTGTTCTTTTGTTGTGGTAGTTATACTATTACTAAACCTTGTTAAATCGATATTTAAATTGAATTTTGAAACAAGCTCCTTGATACTTTCAAGAGGGCTTTTTTTAGTATAAAGTTCAATGAAAGTTATTAAATTTCCGCTTTTAGAGCAACCAAAACATTTAAATATTTGTTTTTCTTGTGACACAACAAAAGAAGGAGTTTTCTCACCATGAAATGGGCAACAAGCCACATAATTCTTTCCTTTTTTTGTCAAAGAAATATATAAAGAAATAACATCAACAATGTTTAACTTTTGTTTAACAATTTCAAATATATTATCTTCTGCCATTTAGCTCCTTTTATTTTTTATAATTTTCAAAAAACTTAATTAAGTTGTCTAATTCAACTATATGTTGTTTCATAGAATCGCGTTCTCTGACAGTTACTGTATTTTTTTCTAATGTGTCAAAATCTATTGTTACTGCAAATGGTGTACCAATTGCATCTTGACGACGGTAACGTTTTCCTATCGAACCGCTTTCATCATAAATTAATCTTAATTCAGTTTTTTGTAATAAATTATCAAATATTTCTTTAGCCTTAGAGTTAAGTTTTTTCATTAACGGAAAAATCGCAACTTGATATGGTGCAATATTGAAATCAAGTTTTAAAACAATTCTTACATCATTATTTTCTAGTTCTTGTTCTTCATATGAATCTACAAGCAATGCAAGCAATAAACGATCTAAACCAATTGAAGGTTCAATAACATGAGGAATTATTTTAGTTCCATCGTCTAACAAATATTCAAGAGATTCATTTGAATATTTCATATGTTGGCTTAAATCATAATTGCCACGATTTGCAATACCAAGCAATTCTCCTCAACCAAATGGAAACAAATATTCAATGTCGCTAGTACCTTTTGAATAATGGCTTAATTCTTCTTTTTCGTGTGGTCTAATTCTTATATTTTCCGACTTTAATCCTAAAAGTGTAACAAAATCAACGCATTTTTTAATTCAATAATGGTAAAACTTTTCATCTTCACTAGGATTGCAGAAATATTCTAATTCCATTTGTTCAAATTCCCTTGTTCTAAAAATAAAATCCCTAGGTGTAATTTCATTTCTAAAACTTTTACCAATTTGGCCAATTCCAAAAGGTAACTTAGCTCTAGTTGTTCTTAAGACATTTTTAAAATTTATAAAAATTCCTTGGGCTGTTTCAGGACGCAAATATGTTTGATTTGCCGCAGTATCAATTGCACCCATTTGGGTTTTAAACATTAAATTAAATGGCCTAATTGAAGATCATTCAGTTTTTGAACCATCATATTCCTTAACTTTTTCTTTAATTATTTTTTCAAGTTCTTCAAATGTTGCTTTTTCAACATTAATTTCTGGAAATAAATCTTTAACAATATGGTCTGCCCTATAACGTTTTTGATTTAGTTTGTTTTCAATTAAAAAATCTGAAAAATTGTCTAAATGCCCACTGGCTTTTCAAACTTTTGGATTCATAATAATTTTTGAATCAATTTGAACATTTCCTTCTTTGGCAATAAATTCTCTTACTCAATATTTCTTAATCGCAGTCATAATATTAACTGCTAGTGGCCCATAATCCCAGGTGTTTATTAAGCCTCCATAGATTTCTGAATTTTGATAAACAAAACCAGAAACTTTTAAGTGATTTATTATATTTTGAATATTTTTTTGTATTTTATCTCCCATAAAAACTCCACAATTTTATTTTATATAAAATAACAATGAAATTTTACCATATTTTTCCTTTTTCATTATTCAAAAAATAAAAATGATCATTGCATTTTTCGAAATTGTTTCAATAATATATTGCTTTGTTAGTATAATAATTACATGTTAGATTTTATTCAAAAAAGAATGCAAAATTCAATACAAAAAATTAATAAAAAAATGTCTATTAATGAAGAAGACATTTTAGAAATTTTAAGAGAAATTAAAATATCGTTATTGGAGGCAGACGTTAACCTTGAAGTTGTTAAAAACTTCACTAAAGAAGTAAAGGCAAAAGCCCTTTCTTCTCAAATTATTGGAAAGTTAAATCAGCAACAAACAGTCCTAAAAATATTCAAAGATGAATTGACTAGAATTTTAGGTTCGAAATCAATTGATATTAAAACAAACAATAACCCTACTAAAATAATGATGGTAGGATTGCAAGGTTCAGGTAAAACTACTACTGCTGCAAAATTGTCTGTTTATTTCAAAAAGAAGAATTTGGCAGTAAACCCTTTGCTTGTTGGTGATGATATTTATAGACCTGCTGCCAGAGAACAATTGCAACAATTAGCAAAACAAACTCAAACAGATTTCTTTACTATGGAAGAAAACAATGCAATTAATATTGCTCAAAAAGCAATAGAATTGGCAAAAGAAAACAAAAACGATTTAGTAATAATCGATACTGCCGGAAGATTGGCAATTGATGAAAATTTGATGCAAGAACTAAAAGATATTAAAAAATATACTCACCCTGATTATATATTTCTAGTAGTTGATGCAATGAGCGGACAAGATGTTATAAATACAGCAAAAATTTTCCACCAAGAACTTAATTTAGATGGCACTATTATCACAAAATTAGATAGTGATGCTCGTGGTGGCGCAGCATTGTCGATTACTCATTTATTGAACGTTCCAATAATTTTTATTGGTACTGGTGAAAAATTAACTGGTTTAGAATTATTTCATCCAGATAGAATGGCAGATAGAATATTGGGCATGGGCGATGTTCTAACATTAATTGAAAAAGCCTCGGAAGAAGTTGATGAAAAAATGATGAAAAAAATTGGCTATAAAATGCTTTCTGGCCAATTCAATTTAATGGATTTAATGAATAGTTTAGCTCAAATTAAAAAACTAGGTAAGATGAAAGCAATCTTAAGAATGATTCCCGGAATGGCAGATAAGGTTTCAGACGAAAAGATTGAAGAAGCAGAAAATAAATTTAGAATTTATACTTATTTAATTAATTCAATGACGAACTTTGAGAAAAAAAACCCGCGAGTTTTAAAAAACCCAAGTAGAAAGCAAAGAATTTTGCAAGGTTCTGGTAGAAGCCCAAGAGAATTTAATATGCTAATTTCGGATTTCGAAAGAATGTCTAAGCAAATGAAAGAATTAGCAAACGGAAATCTAAAATTAAAAAATGGCTTGGATGAATAAAAAAAGTGCCTATTGTTTGGCACTATTTTTTTAACTAAATAATTAGCCATTTAAAATATAAAATTGCAAAATAATAACTAAAAAATTCTTTAAAAATAAAAAGCACTTATGGGTGCTTTTTATAATTTATTCTTATTTTTAATTGAAATTAAGACTAATATTGCTTTTGATATTTTTAGACTTGATAAAACATATCTATCAAAAATAATAATATTTCTTAATTTAAATATTGAATTTAATTTTTTAGTTTTTACAATTAGGTTATATGGAGAAATAGTTTTAATTTTATTGATGAAGTGATTAAATAAATTATTTAAAAAATATGAATGATTTTTAAATGTTTCTAAAATATATTTGATATTTTCAGAGGCTATTTTTTTATCGCGAGGTTTTAATTCTTGACAATTTTGTTTTGAAAAATAAGTATTTAAAATGAAATTGATTGTTTCTTGCATTTCTTCATCTGTTGAAATTTCAATAAATGCCTTATCTAATAAATAAATTAAATTGTATTTATTATTTACTATTAAATTAGTTCATAATTCATCAAAGGTTTTTAATTTTGCATAAATATCGCTATAGTTTAAAAGTGTTTCTACTATAAAATAAATAAATTCCTTAAATGTATTCAATTGAGACAAAAATTTCTTAATTTCTATATATTTTTGATTATCGTTGAATGAAAATAAAAACATTAGCAAATCTGTATTTAATGATTCTTTTGCAAACGTTAGAGCAATTTTTTTAATTTCTTCTTTGTTTTCTTCTATTATTCCTGCACTAAAGAATTGTTTAAATACATCATATATTAAATATTGATAATTGCCTAGAATATAATTTCATTGTTCACGCAAATCAATATTAATAATTGTTTTTTTGGTTAATAATACATCTTCAAGTCTTGATTGTAATCTGAATAATATATCATCAATTTTTCCATCTTTAAATAACGTTAAAAATATAGCTTTGCTATTTGTTTCGTTGCTTAAAAATGCATCAATCAACTTATAAAATTCATACTTAGTTTTTGCACCAAAAAATTTATTTTTTAAGTAATGTCAAGCAATGTTATGAATATCATAAGAAGAATATCTATTTATTAATTGAATAAATTCTGAATTATTTAAATGTTTGTATAAATATCTTTTTAGAGATATTTCTAATTTGCTATCTATTAATATATTGAAATTCTTATTAATTCCATAGATATTTTCTAGTAGGTCTAAATCATTGTTGTATAGTGAAAATAATTGTTGATCTTGGTTTTCAAGCGTTTGATTTCAATATTTAGAATCGCTACAATATTGAATTAAATTAGACATTTTAAATCCATTAGTTAAACTTAATTTAGCAAGTAAATCATATGCAACTTTTTTATATCCTTTTTCGGTGCAAAACCAACTAAATAAGTTTTCATACAAATAATTTTGATGTTCTTTTCAATATTTTTCTTCATAAATATCTACATATAAACATTGGCATTTTTTAGCTACTTCTTTAGCGACATTGTTTAATAAATCTTGTATCATTTCAACCAAATCAAATTCATTGTTATATGAAGAAGAAATGTAAATCGAATTAATTAAATCTTGCAAGGCCATAAATGGTTTGGCATAATTTGTTACCACGATATTTGCTAGTGGATTAATTTTTTGAATATATTCAACAAGCTTTTGGTATTTTAAAATAATTTCTTTGCCGATATTTTCAAAATCTTTTTTTATTTCAATAATTAAATTGGTTTTTGTTTCATTTTTAGCTGTTATTAAATTAGTTAATTTATAAAATGGCATTTTAAAAATTAAATCGCTAAATCCGGCTGTAATTGTTATAAAATTTGCCTTTGAAATAGTTTCTTTATAAAAAGAAAAATTTTTTTTAGAAATGTTTCAATTTAAAAAAATTCATCAAAAAAATTCTTTGAAGGATTTGAAACATGCCAATCAATTGATTGAAGCAAATCAAGACTTTTTGAATTTGCAATTAATTCATTTTCATTATTTGAAGTTAAGGCATAAATTATGTCTAAATTACCCGCTGGTATTGCTAGATTGTAAAAACTTGTTAATCTATAGTTTAAATCTCGCAATTTTAAGGCTAAAAATGATGGATAACCCAAACCATCTATATTACCATTTGATAATTTTCCATTAGTATTAAACCCTACTTTGCTATTAAATCCCGCTGCAAATGAATCTCCTAAAGCAACATATTTAATTTCGTCATTTAAATTGTATTTTTGATTTTTAGCCATTTTGTTCCTTTTCTTGACATTGATCATAAACAATATTATTTAGATTATTAAAATAAGAGATTAATTGAACTATTGCTTTTTTTATATCGGGTAATTGATTTTTTAATATTCAATTATTATTTTTAGCATATAAACTCAATTGGCTAAAGATTTCTTCTTCATCTTGACTTGGTTGCAATTTTATATCAATAATTTTTTGAGGATAATACTTAGATATTAGGCGGTAAGTAGCAACAATAACTTCATTTATATTTAAAGCTTCCAACTTTATTGAACTAACTACAGCTAATTTGGCACCTGTTTCTTCATCTTTTAGTTTTGGCATTAAAAGTCCCGGTGTATCTAAAAGTAAATAATTTTTGCAATTTATTCATTGTTCTGATTTTGTTACTCCAGCCATTGCACCCACTTTTGCAACTTTAGAACCAGCCAATAAATTTATTAAGGTACTTTTACCAACATTAGGAACGCCAAGCACGACACATTTTAATGCAGGTGTTAGCTTATGTTTCTGTGCATTTTTTTCTTTTACTTTTAAATAAATATTATTAAGAGCTGAATTTATTTTTTTATAGGCTAATTTATCTTTCAAATTAGTTGTTACAACAACACCTTTCTTTTCATATTCATTTATAAAAGGTTTTAATTTAGATAGATCTGCTTTGTCGCTTTTGTTTAATATAAATAAAATTGGCTTATTATTAGCCATCTTATAAATATCGTTATTAAACGAAGTAAGAGGTAAGCGTGCATCTAAAACAATTATAAATAAATCAAATAATCTTATTTTTTCATTTAATAATCTAATGGTTTTGGCCATATGACCAGGGAATCAATTAAACATAAAATTTATCAATCTTTCCTTAAAATAAAATCCTTCCCTTTTTGACTTTCAAATTTTGATAGTCCAGCGAAATCATTTTGTCTTAATACTTCAAATAGTACTATTGCAACACTATTGGCTAAATTTAAACTACGACATTCATCAAACATTGGGATACGCAAACAATTAGGAATATTTGTTTGCATAATTCTCTTAGGAATTCCTGTGCTTTCAGTTCCAAACATAATTCATAGTTCACCATTTGTTTGCAATTCTTGTTTGTAATCAACATCTACGTAATTTTTTAAACCGTATCTTGTTACATAATAAATATTTTTATCACCATATTTTTTAAAAAATTCTTCATAATTTTCATGAACTTCATGTTGAATTTCAGTTAAAAAGTGCCCAGCAGCCTTTCTTTTCATTCAATGAGGATGAAGATCAAAGGCAATTGGTTTAATAATATGCAATTTTGCGTTGCCGGCAAAGCATGTACGAATTATATTTGATGTATTTGGGCTTATTTCGGGTTGGTATAAAATAATGTTAATCATGTAAAGTATTTTACTACAAATTGGCATTAAAAATAGTAGATATTGTAATTTTAATTATTTAAATAATTAAAAAAACTTATATAGATCAAATTCAAAAAAGCAGATTTTAAAAAATTTATAACTAAATATTCATTTTTATTGTAGCTACATTTTTATCTTAATATATAAAACAAAATAATTACAGAAAATAAGAAAAAATAGCGAAAAAATCGCTTTTTTTCTAAACTAAAATAAAAAGCACATTCGTTGTGCTTTTTATTTGCTTTATTTTCTGATGTTTAAAGCTTTGATGGTTTCTAAATATAAGTTGTAGTTTTCGTCTTTTAAGTGATTTAGTAATGCTTTACGGTGATTAACTTTAGCAATGAATCCTCTCATTGAATGTAAATCTTTTTTATTTACTGCAAAATGTTCTTTTAAACTTTCAATGTCTTCAGTTAAAATTGCTATTTGTACTGGTAAATATCCAGTGTCTTTTGCATTACGTCCGAATTTAACAACTAATTCAGCTTTTTTTTCTTTTGATACCATATTTTTCCTTTCATAGCCTAGCAATAATCTTAATTATTACCAAGGGATAAAATTGTTATGAATATATTTTAACATAAAAATAAAAAAAATACGCAAATGCGTATTTGGTCAAGTTAATGAAATGGTGCGAACGAATGGACTTGAACCATCGACCTCACGATTATCAGTCGTGTGCTCTAACCAACTGAGCTACGCTCGCATATAAGGAGTTTTAAAAAACTCCATAATATTATAACACAAAAACAAATTAACGTTTTGAAAATTGTCTTGCACGTCTTGCTTTTCTTAAACCGAATTTCTTACGTTCTTTAACTCTAGCATCACGTGTTAAAAATCCTGCATCTTTTAATTTATTTCTATATTCGTTATTAGATGCTTCTAATAATGCTCTAGCAATACCTAATCTAATTGCTCCAGCTTGTCCTGTTAAACCACCACCATTAACATTTGCAAAAACATCAAATTGGTTAGCAGTTTCAGTTACTGCAAAAGGACTTAAAGCATCTTTTATTAAAATTTCTGAATTTAAGTATTCTTTTGCAACTATTCCATTAATAATTAAATTTCCACTACCTGGTAAAATATAAACTCTTGCTACTGAAGATTTACGACGACCTAAACCATAGTATCTTACTTTATCTGCCATAATTATTTAACCTCTAATTTAACTGGTTGTTGTGCTTCTTGCTTGTGTTCAGAACCTGCATAAACATATAAATTTTTAAATTGTTTACGACCTAATTTGGTGTGTGGAAGCATTCCTCTAACAGCTTTTTCAACTAATAATTCTGGTTTTTTTGCTCTTAAATCAGCAGCGGTAATTTGTTTTAATCCACCTGGGTATCCAGTGTGGTGGTAATATACTTTTATTTCATCTTTTTTAGCAGTTAAAAGTACATCTTTAGCATTGATCACAACCACATTGTCGCCCATATCAACATTAGGGGTAAAAGTTGGTTTATTTTTACCTCTAAGTACAGAAGCTACAAGTGCTGAAAGTCTACCTAAAGGTACGTTGGCTGCATCGATAATGAATCATTTTTTATCAACGATTTCTTTTCTAATAATTGTTGTTTGACGCATACTTTCTCTTTCTTATAGTTTGTTATTTTTATAAAATGCTTACCTATTATACCATATATAGTTATTATTAAAACATTTTTATTTAAGTTTTAAATATTTAAATAAAACCTGTTTAATTTAATTTTGAAAGCATGTAAATTAAGGCGGTCTCAGCCCTTAAAATTGTTTTTGTTAATGAAACAACTTGAACATTGCAAGATATAGCATAATCAATTTCTTGTTGGCTAAATCCACCTTCAGGGCCAACAATAATTAAAGCATCTTGGCAATCTTTAATTAATTCGTTATTCTTTTTATTTTCATAAGCTAATACTTTATTTTTAATATCTAAGACACAAAGTTCTGAAAATGAAATTGGATCATGAAGAATTGGGACATAATTTCTAAAACTTTGCTGGCAAGCTTCAAAAATTATTTTTTCATAACGTTGCTTTTTGGAATCAAAATTTAAATTAGATTTATCAACATATTGGCTTTGAAATACATAAATTTCATAAGCGCCAAGTTCAACTGCTTTTTGCAATGCTATTTCAAAATGGTTTTGTTTAATTAAAGGAATTGCAACTGAAATTTTATAATCCAATTCATTATTAATATCTAACTCTTTAACTAATTTAGCACTATTAGGAAAAACAAATTGACATTCATAAAACTTATTTTCATAATTTACAATAATATTTTCTTTGTTAAAACGTAAAACCTTAAAATGCTTTTGGATTTCATCACTAAAATAAAAAATTCCATCTTCTAATTTGTTGGCAAAAAATTTATACATATAAGTATTTTAATATAACCAACACAATAAAACAAAAAAACCCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTACTAAATAATTAATATGTAATCTAATTGCTAAAAATTATTTTTTTAATAATTTAAATTAATTGTTAGATGAAATAATTTTTATCTTTGTTTTGTCTATACCAGTATTTTCTCAATTTATTTTACTTGCATCACCTTTATAAATTACTTCTTTAAGGTGTGTGCAATTATAAAACGCATCTTCGCCAATTTCTTTTACTGAATCAGGAATAGTAATAGATTCAATTTTTGTATTTAAAAATGCCCTAACTCCTATCTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTCTACAACCATAAAACGCTCATTTGCCAATTTCTTTTACTGAGCTTGGAATAGTAACTTTTGTGGTTTTTTTATCCGAATATGTTAATATTTTTTTATTATTTTTATCAATAAAAAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTAAAATACTTGAAATATTTCCACTAGATAATGCGCCTTCTCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTTAATACTTTTATTTTGAAATGTCTCGGCGCCGATTTTTTCAAGACCTTCATTTAAAATTACTTTTTCAAGGTATGTGCAACCAGAAAATGTACTTTTGTCAATTTCTTTTACCGAACCTGGAATAGTAATAGATTCAATGTTTGTACCATAAAATGCATCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATGACTTCTTTAAGGTTTTCGCACCAAGAAAACGCACCTTCGCCAATTTCTTTTACTGAACCAAGAATAGTAATAGATTTAATGTTTGTATGATTAAAAGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCATTAAGGTTTGTGCAACCAGAGAACGCACTTTCGCCAATTTCTTTTACTGACCGCGGTATAACAATATATCCAATGTTTGTATTAAAAAATGCCTTAGCGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATGTTTTTGCAACCAGAAAACGCACTTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGCCTTAGCGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTTCGCACCAAGAAAACGCACCTTCGCCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATCATAAAATGCCTCAGCGCCGATTTTTTCAAGACCTTCATTTAAAATTATTTTTTCAAGGTTTGTGCAATCAGAAAACGCATTTTCACCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATTTTTGTATTATAAAATGCCCCAGCGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAAGGTTTGTGCAACCAGAAAATGTGCTTTTACCAATTTCTTTTACTGAACCAGGAATAGTAATAGATTCAATGTTTGTATGATAAAATGCCCCAGCGTCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTTAATATTTTTGCAACCAGAAAACGCTCCTTCGCCAATTTCTTTTACTGAACCGGGAATAGTAACTGATTGAATAACACTATGAAAAAAAGCACAATTACCAATTTCTGTAATATTTTTGCCTTGATATTCCTTAGGAATTGTTACTTTAGAATCATTTCCAATATATTTTGCAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAATTTTTTATTTAAATTTGATGAATCATCTTCTTTGGTAGGATTTTTTGGTTTAGGTTCTGGTTTATTTTCATCAGGCTTTGAATCTTCTTTTGTAGGATTTTCTGGTTTGGGTTTATTTTGATCGGGCTTTGATTCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTTTATGATCGCAAGAAATAGCTGCTAATGGAAGTATAGCAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATATTCTTTTCCTTTGTTATATTAATTATTATATTTTATATATCTATTTAAAACAAATAAATTATTAAAAAAATAAAAAAATTCTTTTTTTGAATGAATTTGGAAGCGTAGAAAAATGTGGGATTAATTACAAACAAGAAAAAAAGCAAGATCATAAAGGTCTTGCTTTTTTCTATCATTCATAAAAAAATAAGTTTAAAAATTTTTGAAAAGAATTTTATGGGTGGCAATAAAGAATTTATGTTAATGAAGCAAGAGGCTAAATCAAACAATGATTATAGCCTTGTGGATGCATTTAAAATAAATTTAAATTGGAAGAAAAATTATAATAAAAAATCTATGACAAATTAAGCAGCAAAGCTATAAACAAAATTTTAAAATCAAAAATAGTTATTAGTGAAAATTATTATCAAAAGCTAAAAACAAATAAAGTGTTGCTTGAAGTTTTTATAGTTATTAAATCCTTAAAAAATCCGTCTTCTAATTTGTTAGCAAAAAAATTTATACATATAACTATTTTAATATAACCAACACAATAAAACAAAAAAACTCGTAACTTTTTTATAAATTACGAGTATAAATTTTTTGCATAATAATTAATTATATGTAATCTAATTGCTAAAAATTATTTTATTAATAATTTAAATTAATTGTTAGATGAAATAATTTTTGTCTTTGTTGTGTCTATACCAATATTTCATCAATTTCAATTTATTTTACTTGCATCACCATTATAAGTTACTTCTTTAAGGTTTTTGCAATTAGAAAACGCTCTTCGGCCAATTTCTTTTACTGAATCTGGAATAATAATAGATTCAATGTTTGTATTTGAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAACTTACTTCTTCAAGGTTTATGCAACTAGAAAACGCTCTTTGGCCAATTTCTTTTACTGAATCTGGAATAATAATAGATTCAATGTTTGTATTTGAAAATGCCTCAGCGCCAATTTTTTCAAGACCTTCATTTAAACTTACTTCTTCAAGGTTTTTGCAATCAGAAAACGCACTTTCGCCAATTTCTTTTACCGAACTAGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATGAAGAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATATGCGAAATTATTGAAAAATCAAATGCCAAAGCGTCAATTCATTCAAGACCTTCATTTAAAATTAATTCTTCAAGGTTTTTGCAATCAGAAAACGCTCCTTTACCAATTTCTTTTATTGAACGAGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTTTTTTTATCAATAAAAAAGTTATCTTTAATTTTAAAATTTTTATTATATGAATTAATTGAAATATGTGAAATTTTTGAAAAATCAAATGCCAAATAGCCAATTTCTTTTAACGAGCTTGGAATAGTAATAGATTCAATTTTTATAGTTCGAAATGCCGCAGCGCCAATTTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGTTTCCGCAACCATAAAACGCATCTTCGCCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATGAAGAAGTTATCTTTAATTTCAAAATTTTTATTATTTGAATTAATTGAAATACTTCGAATATCCCAAATAGATAATGCACCTTCGCCAATTTCTTTTACTGAACCGGGAATATTAATAGATTTAATTCTTGGACTTGAAAATGCCCTAACGCCTATCTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGTTTCTGCAACCATAAAACGCACCTTTACCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAGAAAATTATCTTTAATTTCTAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTTTTGAAAAATTAAATGCTAAACTGCCTATTTCTTTTACTGAATCAGGAATAGTAATAGATTCAATTTTTGTATTTAAAAATGCCCCGGCTCTAATTTTTTCAAGACCTTCATTTAAAATCACTTCTTTAAGGTTTCTACAACCAAAAAACGCACTTTCGTCAATTTCTTTTATTGAACTAGGAATAGTAACTTTTGTGGTTTTTTTATCCAAATATGCTAATATTTTTTTATTATTTTTATCAATAAGAAAGTTATCTTTAATTTCTAAATTTTTATTATTTGAATTAATTGAAATATGTGAAATTTTTGAAAAATTAAATGCTAAACTGCCTATTTCTTTTACTGAATCAGGAATAGTAATAGATTCAATTTTTGTATTTAAAAATGCCCTAACGCCTATCTTTTCAAGACCTTCATTTAAAATTACTTCTTCAAGGTTTCTACAACCCAAAAACGCTCTTTCGCCAATTTCTTTTATTGAACCAGTTATAGAAACCGAATTAATAAAACTATTAGAAAAAGCACCATTACCAATTTCTGTAATATTTTTGCCTTGATATTCCTTAGGAATTGTTACTTTAGAATCATTTCCAATAAATTTTGTAATTTTTCAAGTATTTGATTTAGTTAGTTCAAATTTAAAATTTTTCCTAAAATTTAATAAATCTTCTTCTTTGGTAGGATTTTCTGGTTTTGGTTCGGGTTTTGGTTCTGGGTTAGGTTCAGGTTTATCCTCTGGTTTAACTTCTGGTGTTGTTCCTGGTTTATCCTCTGGTTTAGGTTCTGGTTTACTTTCATCAGGCTTTAAATCTTCTTTGTTTTCTTTTGAATTTTGTTTTACTCCATCACCACAAGAAATAGTTGCTAATGGAAGTATAGCAGTTAATGCTGAAATACTACTTAATATAAGTCATATCTTACTATTTTTTTTATTTTTCATTTTCTTACCTTTCATTATTATTTATAATTTTATTTTTATATATATTAAAAAATAAAACCTCTTAATAAAAAAAAAAAAAAAAACAGATTTTTAATATAATTGGTTGAAAAAATGGCTTATTTTCTATATTTTTTATGCAATTTATATATTCTTAAGGTCAAAAAAATGCAATAAAAAAACTCAGATTGAACTGAATTTTTTTATTTTTTAAATGGCAATAAAGACAATTAATTGCATTTTTTTAAAACAGATTTCTTACATCATCTTAGTGTTTTAATATATCTGCAAGTTCATCGTTTGAATCTTCATCATTATCCATTGAAAAAAATGATTGTGTTTTAAATGTTCCTTCAGATACAAAGAATTCCTTTGTTTCATCGTTAACATAATTTAAATCGTCTTTTTTTATTACATTGATTACTTCATCTTGAATTTGATCAATGTCTTTAGGAGCGTCTTTTCTTAATCCCGTGGCGATTACCGAGACAAATAGCTCACCTAATTTGTTGCTTTCTTCAGATTCTAGAGTTGTTATACCAAAAATAATGTTAATTTCATTACCAACAATTTCCCTCATAGCTCCCAAAGCACTTTGAATTTCATTTAAAGTTACTTTTTGAGAAGCACTGAAATTTACTATTGCATCACTAGCCCCAACAACACTTGATTCTAAAATTGGGCTTGTTATTGCAGATGTGATTGCTTTTACAGCCCGATCTTGGCCATTTGCTTGGCCAATGCCGATTACTGTTTCACCTTTATTTTTAATAATGGTTGATAAATCTGCAAAATCAAGATTAATAATGCTTGGGGTGGCAATAACATCAACTATTGTTCTAATAGTTTGTTTTAAGACATTATTTGCGCAAATAAATGCATCTGAAAAAGAAATATTTCCATATTGTTGTAATAGTTTATTGTTTGAAATAACAATATATGAATCAACACATTTTTTAATTTCTTGTAAGCCTTGCTTTGCAATTCTCATTCTCTTTGGACCTTCAAAGTCAAACGGAGTAGTTACAACTGCAACTACTAAAGCATTGCATTCCTTGGCTATTTTTGCAATCACGGGCGCTGCACCTGTGCCGGTTCCACCGCCCATTCCTGCAGTTATAATTACTAGATCAGCTCCTTGCAATCTACTTTTGATTTCTTCACGTGAAGATTCAGCAGCAGTTTTGCCAATTTCAGGGTTAGCACCCGCGCCAATTCCACGTTCATCACCTAAATGTAAAACGCATTCTTGAGGAAATTTTGCTAATACTTGTTTATCGGTATTGGCAGCAATAATTTGAAAACTATCTAAATGGCTATTTATCATAGTTTCAACACTATTATTTCCGCCCCCACCTACTCCAATAACAATTATTTTTGCCACCGGGTTATAATTATTTAATTCTTTTGCCATAGAACTCCTAATTTATATCGTTTAGCATTAAATTGTAATACTTTTTGGAATTACTATAAACTTCAATTGTAAATAATTCCCTACTAATTTCATTATTTAAAGCATTTTGTTTGATTTGACATTCTAAAATATTATCTATTGCAAGCATTTCGCAACTTAAATATTCATTTTTGATTTCATATTCAAAGTCACTAAATAAGTTAATTTTTTCATTTTGAATTAATATACTATTAAAAAGTTTTAATTCGTTGTTGAAATATAATTTGCAAACCTTTGAATCAATTTTATAATTATTAACAAAAGTTGCAACGCTAGTTAATAATTTATTTATAAATTCATTTAATATATTCTTTATTATTGGAGCAAAATTGCATTTGTAGGTTAAACAATTTACAACTTCACTAAATCAATTGCTTTCTGTACTTAATAAATTGCATTGTAACATTCTTGCTTTTAATTTGTTTAGTCCTAAATCTAATACTTGGCTTACTATATTTGATTTATTAATGTTTAAAATAAATTTAGCTTTATCATCTAATACATCAACAAAAATAGCTACTTTTTGTTTTGAATTTTTAAACAAAACTGAATTTGTCTTTAAACTATCTAAGCAAAATATTTTGGCAATTTTTAAATCGTTGCTACTAAATATTTCTTGCAATCTTAGACTATTAGCATTTGATATTGTTTTTAAAGAATAAACTTGCTTAATATTAGTTTTTTTAACTAGTTTAAATTCCAATATTTTTTGGCCTATATTATTGAAGTTTGATCTAATATTTTTTAAATTACTAATATTAAAATTGTCATTATTTAAGGTTAATAGTTTACTATTTATATTTCATTTGCTATCTTCAGACAACATAACAATAACTTCATTTATGCTTGCTGATATTAATTTTTGAATTTCATTCTTTGTTTGCTTTATAAAATTTTTTATATCGCTTTCAGTTAAATAAATATTATTAATTGCTTTTTTATAAATACAAAAAGATAAAGTATTAGTTTTTTCCATAACTTCTACTTCAATACTATTTTTTAAAAATTTATATGCAGCAATAATAATTTTTTTCATTTAACTAATCCATTAATTTTGTAATAACGCGTAATTTTGCGCTTCTTGATCTTTTGTTGATTTCTTCTTCTTTACTACTTGGAAAAATTACTTTTTGATGTCACTTATGAGTTTCAACAATTAGTATTTTTTTTAACTTAGGGTCAAAATAATTTAAATTTTGATAAAACTTTTTAACTATAACGTCTTCTTTACTATGAAATGTTATAATCGCTATTTTTCCGTCTTTTTTTAATAAATTGGGAAGTTGGATTAGCATTTCACTTAAGGCATTTAGCTCATCATTCACTGCTATTCTAAAAGCTTGAAAAACTGCTTTAGAAGGATTTTTTTGTCTTACTATTTTAGCTGGCAATGAATCCTTTATTATTTGATTTAACGTAAATGAATCAGATATTGGTCGATTTTTTACAATAGCTTTCGCAATTAAATTACTTAATTTTACATCAGCATTAGATTGCAATATATCTATAATTTCTTTTTCAGTTGCATTATTAATAATTTCTTTTGCAGTTAGAGTATTATCTAAATCCATTCTCATATCTAAATCAGAATTGATTGAATAAGAAAACCCTCTATTTGGATCATCTAATTGAGGACTAGAAACTCCTAAATCTGCTAATATTCCATCGACTTTATCGATATTTAGTTTTTTTAGTTCACAAGTAATGTTTCTAAAATCACTTTTTATTAAAATAAAGTTTTGGCTTATTTTTATTAATTTTTCTCTACTTTCGTTTATAGCTTGAATATCTTTATCAAAACAAATTAATTTTCCAGTTGTTAGTCTTTTTAAAATTTCTTGGCTATGACCACCACGTCCTAATGTTAAATCAACATATATTCCGTCTGGTTTTATTGATAATTGTTCTATTACTTCATTTAATAGAACTGGAATATGTTCCATTTAGAACTTAACTCCTGCTTCAAATAATTTTTGAGCCAAGTCATTTACATTATCATCATTTTCATAGTAGCTTTCTTGTTCATCATAAACTTCTTTAGCAAATAATTCACAAGTATCACCAGTTCCTAGGAAAACAACTTCTTTGTCGATAGTGGCTTTATTTAAAAGATGTTTGGGAATCATGATTCTGTTATTTTTATCAGGAGATACAACTTCAGTATTTCCAAAAATGTAGCGAATGAAATCTCTTACGTTTTTGTTAAGTGTGTTTCCTTCTTCCAATTTAGTTTTCATTTTTTGAAACTCAGAAACCGTTCTTAATACTAAGTTTTTATCTATTCCAATTGTGATATAGAATTCTTTGCCTAATTCTTGTAGTAATTTAGGCGGAACAACAACTCTATTTTTATCATCAATTTGGCGATAATATTTACCAAACATTCCCACATCCTCCCACTATCAACTTAATTCTAACATATAAAACAAGTATTTTTTAATTTTTTATAAATAAAAATAAAAAAAGAGGGCAAAAAAGCATTAGCCCTCTTGCAATAGAACCATAGTAATTATTTTTTTAATTGTTTTATTAAAAAATAAATAACTGTATAAGTTTTTTGTTAAAATTAAAATATGCTAGTTTAGCTCAGTTGGTAGAGCAATTGATTCGTAATCAATGGGTCGGGGGTTCGATCCCTCTAACTAGCACCAAATGCACAAAGTAAGCGCCAAAAATAAAAAATAAAAAATTTATTAAAAAATTAATTTTTTTTAGTTATAATAATTAAGCATTTATTAAGTGCCGACTTAGCTCAGCGGTAGAGCAGCTGGCTGTTAACCAGTTTGTCGTTGGTTCAATCCCAATAGTCGGCGCCATTATGGCCCTATGGTGAAGCGGTTAACACATATGGTTTTCATCCATACATTCGTGGGTTCAAGTCCCGCTAGGGTCACCATTGGAGAATTAGCTCAGCTGGGAGAGCATTGCCCTTACAAGGCAAGGGTCGTGGGTTCGAGCCCCTCATTCTCCACCATTTGTTTAAATACACCGCAAAAGGCCAATCGGCCTTTTTATTTTTCATTTAGATTTAAATTAAATGTAATTTTTATATAAAAAACTCGTAACCTTTTTGATTACGAGTTTTTTATATCTAGTTTTTAATCATCAAATTGTGAATAATATAGTCTTGCATATTTTTTATTTAATTCTAGCAATTGCTTATGGGTTCCTTGTTCAATAATACGACCATCATCCACAACTAAGATTAAATCAGCATTTTTAATCGTGCTTAGACGGTGGGCAATAACTATTGATGTTTTACCAACCATGATATTTTCTTTTAATGCTTGTTGAATTATTTTTTCTGTATTAGAATCCACGTTCGATGTTGCTTCATCTAATATTAAGATCTTTTTGTTTCCCAATATTGCTCTAGCTATTGAAAGAAGTTGCTTTTCTCCTTTAGATAATATTGAAGTGCTATTATCAATTATAGTATCATATCCATTTTCTAATTTTTCAATAATATGATGAGCAGATGCTAATTTTGCTGCCCTTATTACATCTTCTTTGGTTGCTTTTGGATTTCCTACTAAAAGGTTATTTAAAATACTTTCATTAAACATAAAGCTATCTTGTAAAACAACTGCCGTAAAATCTCTTAAACTGTCTTTTGAAATTTCTCTTAGCTCTATTCCATCTATCTTTATAGAACCTTTTTCATAATCATAAAAACGATTCAATAAGTTTATAATAGTAGTTTTACCTGCTCCAGTTTCTCCAACTAAAGCAATAGTTTGTCCTGCTTTTGCATTAAATGAAGCATCTATTAGTTGATATTTAGGCAAATTTTCATTATATCTAAAATATACATGATCAAATGATATATTTCCAACAGGATTTTCTATCACAATTCCCTTTGAGTGGTTTTTTATATGTGTGTTTGGTAGGCTTAAAAGTGTATTAACCCTAACGGCAGATGCAATTCCAATTCTTGCATAAACTAATATAGTAAGCATTGATTGAAGTGTTGATAAAAAACTATATATAAGTGTTAAATAACTTATTAAAAATCCATGTTCTGCATTTGTATTAAAAAGCCCATAAACATGAATATTATTTCCCTTTAATACAAGTGTTACAGTAACTATTAATATAACCAAAATATTGTTAGTAAAAACTATTCAAGGGTTAATTATTTGGTTATTAAGATCTCCTGTGAAGGCTGTGTTAAATATGTTTCTTGCTATTTGTTTACCATGATTATTAACTTGTTCTTGTTGTTCGAAAGTTTTAATAATTTTAGAATTAGTTAGCATTTCTTCGACAAAAGCGTTTAATTCCCCAAAATGTTTTCTGGTTTGAATAAATGCTTTTTGAGCTTTTTTAGTAATTAATATTCCTATAAATGAAAATAAGAATAGAACAGGTAAAACAACAGCAGATAATACTAAGCTAACCATTGATAAAAATATTACAGATAAAATAACATTTATAGCACATTGGATTAATTCGTTTAATAATTTAAATATAGTTTCAGATAAACTATTAATATCATTTACTAAAATAGAAATAATATTACCTGATTTTTGATTATCAAAATACGAAACAGGAATATATAAAAGGTTTTTCATGGCGGTTTTTCTTATATTCTCACAAACTGAAAAAGTTATTTTTACAAAGAATACCCCTGAGACATATCTAAAAATTCCATACAATATAAACGATATAACCAAACATGAAGCCATACCTATAAATAATTTTAAATTAAAAGGTGTTTTATGATCTAATACATCTTTTGTTAAACATATTGTAAAAATATATCCAATTAAAAATGAACCTATTGCATAAAAAATTGCATGAAAAAATGAAAACAAAACAGCCAATCAAAATTTTGTTTTTGATTCACCTGAATATTTAAAAACTAATTTAAACAAAGTAATAAATGAAAGTTTTTGCTTTTCTTTAGTTCTTTTTTTCATCATTGCCTCCTTTTCTATTGGCTTAATTGGTTATTATATGATTCATTATAATATGAACATGATTTCATTAATTCTTCATGATTTCCTTTATCCAACATATTTCCATCAGATAAAACAATAATTTCATCAGCATTTTTTATTAATGGCAATTTTTGGCTAATTATTATAGTTGAACATGAATATTTATTTTTTATATTTTGTAAAACTTGGTGTGCAGTAATATTATCTAAAGCACTAGTTGAGTTATCTAATATTAAAATTTTTGGTTTAGTTAATAAAGTTCTTGCAATTGAAAGACGTTGTTTTTGGCCACCTGAAAGGTTTGTTGCCCCTTGAGTAATGACATGATCTAAATTTTCTTCAAATTTATAAACAAAATCATATGCACAAGCGTCACGTAATGCTTGATTTATTTCTTCATCTGTTGCATCATTTTTAGCAAAAAGCATATTTTCTTTTATTGTTCCTGTATTTAATAGGGCATCTTGGTAGACAATTCCAATCGTTTCATGTAAATTTTTTGTATTAACTTCTTTAATTTCATTATCCCCAATTAAAATCGATCCTTCATTATATAGATAGTTATTAACCAATAAATTTGCAAGTATGCTTTTACCACTTCCAGTAGGGCCAACTAATCCAAGAGTTTTTCCAAACGGTAGGCTAAAACTTATATTGTGTAAAGTATAATTTGGATTTGAAGAGTAATATTTGAAGCTTAAATTTTCAACTTTTAAATCAAAGTTTTTTGTTATTTTTTTACCATTTGATACATAAATTTTATCTTCGTTAGTATTTAATACTTCTATAACTCTAGTAGCAGAAATTTTTCCTTTTACAAATGCAGAAATAAATACTGCCCCCAATATAACACCAAATGTTACATAAAATGAATAGTCAATAAAAATATTAAATCTTACAATTGTATCATATGTTGCTACATTAGAAATAACATCATGAACAACTAATGAATAAAGCACCACAACAAATAAATTAAATATTCCAAAGAATAAAGGAGCAATACCTACAAAAATGCTACCAGCATTAACATTAAAATTCTTTCATGTTTTATTTATTTTTGCAAACTTTTCTTCTCTATTTTTATATAAATCATATATTTTTATTAAACGAGATCCTAATATATTTTCATCAACTTGCTTAGTTATTTCTTCAACGGCTAATTGAGCTTTTTTAATTGAAGGACTTGCTTTTTTGGCCATAATAGAAACTATTGTTAAAATTATCGGAACTAACAAAATTAATCCTATTGCCATTTTTACGTTATTCATGACGGCTAAAATACATCCACCAATTATCATTGAAATAGCCCTTGTTAAGACATTTGTAGCGCTTATTAAAAATTCTCAGAAAACCGCAATATCATCACTCACCCTAGTAATTAATGATTCAGGTTTCAAATCAGATATATTTTTTAAACTTAATGTATTTAATTTTCTAATAAAGTTTTCTCTAATATATCTACTACTTCTTTCTGCTGCATATACAATTAAGCTAGTGCAGCCAAATGTTAAAGACATTGAAATTATAGTTAAAACTATTATAGAATAAATCATATAATTACGAGCATTAATAGCATTATTAAATGTAAAGTTTATTAATCCATTAAAAAAACTTAAATCTACTTGACTATTTTTATTTTCAATAAAAAGCAATCTAATGAATTGAGAAATCAATGTAGGAGTAATCAATTCAAATATAACCGAAATTAAAGAAATAAAGCTTCCTACAATAAACAATATTTTAATTTTTCATGGTAAAAATTTAATAAGCTTCAACATAATTTCTCCTTCTTTTAAATGTTTTTTATTGCCACTTTCAAGGCTAATTAATAAATTATAATGAAACATAAAAAAAAGTAATAATTTAGTGCAAAAAAATACGAAAAAAAAGCGATTTTTCGCTTTTTTTGTTTAATTTATTTTTATAAACTAATTTTATTTATAAATTCAACTTCTATTCCATTTCATTTAGCAGTGATTGTATATTTTATATATTCTTTACCTTTGCTTGTATATTTTTTAACTGAGATTTTTTCAAATGTTAATCCTTCTGGCAATTTGTATTCGAATTTAGAAATATCATCTTTAATTGCTTCATCAACTGTTTTTTTATCGCCAATGTAATTTACGGTTACTAATTTTCTAAAATGTTCTTTTAAAACTATTTTTTGCATAAAGTCTTTATCTTCATTTATCTTTTCATTAACATTAACACGTTTAGATGACATGTTGCTAAAATCATTATTTTGAAAATTTTGAAGAGAAACTATAGAAACTCTAATTGATTTTACTTTGTTATTTTCTATTTTAGGAAATGAAAAGATATAAACATTTTGTACATCAACCATATGTTTTGTATATTCAAATGCAAACATTGAATGTTTTAATTTTAATGTTGTTTTATCATAATCAAAATTTTGAACCGAAATGTAATTATCACCTTTTTCAATCATTTCTTTTTCAATGTTTTTGCTTAAAAGACTAATATTTTTCAATTTTTCTATAAACATAAATGTTGAATTATTTTTAACTTCATCGTTAGATAATTTATCTTTGTTTTCTTTGAAATATTTTAAGAATGCATCTTTATTTAATTCTTTTACATCTTGGTCTTCAATAGTTGATGTTGGGGCTTGTTGGTCTTCTATTTCAGTTTCATTATTTTGTTTTCTAATCTTATTATATTCATCAACACCCAATTTGCTTAAATCAATTTCAACAAATTTTGATTTCTTAATACTATTTTCATAATCTTTAAATGAAACAGTAAGATTAATCAAACACTTATCGCCTTCAAATTTTAATTCACCTAATGTGTAATTAATTTCACTGTTTTTATTATTAATTCAGTATGATTTATTTTCTAATTTGTCAATAGATTTCTTTAAAATGTAATATTTGTCTTTACTATAAAAAGGATAAATTGACGTTTCTAAAGCTTTGTTTAAAAGTTCAGCTTGTTTTTCTGGTGATAGCAATGCAATATTATCTAAACCGTTTGCAAATTTATCTAATTTAACTTCAATAGTTTTAGATTCACTTATTTCTTTATTTAAAAGACTTGACAAAGTTAAAGTCAATGTTATTTTTTTATCTTCAACTTTTAAATTGCTTAATACTACTGAATATGAAGCTTGGTCGTAGCCTTGAACTGCAAAATTTGGTTTTTTATCAAAATTGTTTATTTGTTTATTTTTTAAAATATCTTGATATGACTTGGCATTTGCAACTAAAACATCTGTTTTTTCAATTTGATCTTTTAGTTCTTTTTTGGCCTTATTTATTTTTTCTTGCAATTCTGGAGTTAGTTTAAAACCTTTTATTTCATAAATGTATTTTTGACTTGAAATTTGTTTATTTTTGTATGATAAATAGTAAAGAATTGACAAGTCATATGTTCCATCTTCTCCTGTATTTTTAGTAATTTTTAACGAATCTTTAACAATTGAAAAGTTTTCTTTAATAGTTTTTAAAAATACTTTTGATGTGTCTGCGTCTTTTATGTATGTTTCTTGTTTGTTAGGATAGTCAAAGACTAGATTTTTTGAAACTTCTTTAAAATCTTCTTCTGAAACTTGATCTAATCCAGAATCTAAGTTTCCCAAATTACTTTCATAAACCTTGTTTGAAATAATATGTTTACCATTTTGTTTAAAAATTGCAACCTTATATTTCAAAATAAAGTTTCCTTTTTCATCTTTTGTTAATAACAATTTAGAGTTTAACTTGTCTCCACTTCTAAGAGGTTTTTCAGAATTTACCAATTGAACAGATGTGCCTGATTTAGAATTTCATTTAGCAATAGCAAATTCTTGATTTTTAAAATCATATGCTCCCTCAAAAGCCATGATATTTTTGTTATTTCTTTCAAAAAGAATTTTGTATTTGTCTAGATTATTTAAAACCTTTTTAATAAATTCTAAATTTTCAGCATTTCATGTTATTTTTGAAATTGATTCTTCACCATATTTATCAGCATCATTTTTATTTGTTTCTTCTTCATGACTTGGATCTTCTGGTTTAACTTCAGGTGTTGTTCCTGGTTTATCTTCTGGTTTTTTAGGTTCTTCTGGTTTAACTTCAGGTTTTTTTGTTTTTACGCAACCTGCTGCCACTAATGGTATTGCAAAAACCGAGGTTGCAACACTACCTAAGGCTAATAAAATTTTTGTATGTTTTTTCATTTTTTATATTTCTTTTCCCTTTTTCTTAAATTAATAATTTAAAAATTTATCACACGAATAAAGGCTCAAGTTCAAATGACTGGTTTTTTCTTTAAAAAAGAAAAATAAATAACCGTTTGGTTATTTTTTATTTAAAGATAATGTTTTTAAAAAAACAAAAAAATAATAAATTACAAAAATTGTTACGTTATTTTTTTGATTTAGCAAATATTATAATGTGTTTTTTTTAGCCTATAAAAATATTTAAAAAACTAGCATTTTAAGCTAGTTTTTTTAATATGGTACATCCTGTGGGGCTCGAACCCACGACCCAGGGATTAAGAGTCCCTTGCTCTGCCAACTGAGCTAAGGATGCGTGTTTTTGCGTTATTTATTATAACATAACTAAAACAATTTTTTAAAATTAAACAAATAAAAAGTAGCAAACGCTACTTTTTTGTTACAACTTGATGATATGGTACGCGAGGAGGGACTTGAACCCTCACGCCGCAAAGCATTAGTGCCTAAAACTAACGTGTCTGCCAATTTCACCACTCGCGCATATGGTGCCGTTTATAGGACTTGAACCTACGACCTACTGATTACAAGTCAGTTGCTCTGCCAACTGAGCTAAAACGGCTTTTGTAAATGCTTTATTATTATATAAGGAAATTTTAAAAAATTTTACATTTTTATTCAAATTTAAAATTTTAATTATTCATAATACCTTAAAATAAATGTTGAAATATTATTTTTTACTATTACTATATGATTTTTTTATTAACTTTAATTTTTAAAGATAATAAATTAATATTTTTAATGTATGATTACATGGCAAGGACAAAATTATGTTTTATAAGTTATATAAAAAAAGAGGCCAATCAAGTTTTTTTGCAATAAAAAAATTTGCAAAAAATAATAATATCAATAAAATTGGACATTGTGGAACATTAGATCCTGAGGCTGAAGGTCTTTTAATCGTAGCGACAGATGAAGATACAAAGGCCTTGTCTTTAATTGAAAATCAAAACAAGGAATACTATGTGGAAGCAAAATTCCATTACTCTTCAGATAGTTTTGATTTAGGAACATATGTAACTAAAATACAAAATTTTCAAATAATAACTAAATCTCAAATTATACAAGCACTAGAAATATTAAAAAAACAAACAAAACAAGTGCCTCCAATTTATAGTGCAAAAAAAATTAATGGAACAAGAGCATACAAACTTGCTAGAGATAATAAAGAATTTAGTTTAAAAGCCAATGATATAAAAATAAATAATTTAGAATTAATTGAATTTAATGAAGTTAACCAATCAATCATCATTAAGGCCAATGTTTCAAAAGGCACATATATTAGATCAATGATTCATGATTTAGCTAATATATGCAACACAGATGCAGTGGTGGTCGTTTTAAAGCGAATTAAAATTGGCAATATATGTTTAAATGGTCAAGAAGAATTTGAAAAAATTACAAACTTAAACGAACTTTTTAATGTAAAATTATTTAAGCTAACCAAAAATGATATTCAAATATTAGCAAACCAAAATTCAAATAGTTGCTACTTTGAATCATTGAAAAATTTTTGCGGAAACATTATTTTTATTTACTTGAATAACATAGTAGGATGAGGAAAATCAGAATTTGGAAATGTAACTATTTTCAAGCGATTTTTTAAACGCCTTACGATGTTAATTTCTAGCTAGGAGTAAACATGACAAAATACAAAATGATAGTATTTGATATTGATGGAACATTGTTGCCATTCGGAGTTACTCAATTAACACCTAGAATGCAAGAAATGTTTGAAAAATTAAAAGAACAAGGCTATGTAAATGTGTTGTGTTCTGGTCGTGATATTTTTACAGTAGGCGCCCAAATTGATAACCCTTATGTTGATTATTTTGTAGGTGCAAATGGAACTTTTATTCTTGATCTAAGAACAAAAGAATATATTTTTGAAAAAACTATTAATTACAAAGATTTTGTTGAATTTAGAAAATATGCAAGCGAACATAAATTATCTTTTTCTTTTGTTGGAAACAAATGGGGATATTACAATGAATTATTTAATATAGATCATTGATTTTATAGACCATATAAAGACAAATTTATCCCAGAAAAAGAATTCGAATCGCAACATGATAAAAATTATTTAATTACTATTTCAACTCCAACACCATCTATAGTAGCAGCTGATTTAGAAAAATTCTTCAAAGAAAAAAATCTTGATTTATGAATTCTAGCTCAATGAAATGGTGGCATATTCATATCTGCTAAAGGAATAACTAAGGCTGTAGGTTTAAAAATACTAGGAGATTTATTAAATATTTCTCTTGATGAAATGGTGGCATTTGGTGATTCAGAAAATGATATTGAAATGCTTGAAAAAGTAGGATTAGGTATTGCTATGGGCAATGGAGAAGATGTTACTAAAGATGCTGCAAATGAAATTTGCTTGCCAGTTACTGAAGATGGTCCATATTGAAAATTAAGAGATAAAGGATTTTTTGACTAATAAATTTCAACCTTATGGTTGATTTTTATTTATTTTATTTTAAAATACTTATATTAGGAAAAAAACACATGGCAATTACAAGTACACAAGATCCAAAAATTCTAAAATATAAAAAATTACTTACTCCCAAAGGCAGACAAGAACAACAATTGTTTATTGTTGAAGGTTATCATTTAGTAAAAGAAGCAATCAAGGCAAATATAGTTATTGACATTTTAGAATTCAATGACATACTAACTTATAAAAACTCAACTCAAGTAACTAGAGCAATTATTAAGACACTATCAACAACACAAACGCCTCAAAATATCGTTGCAATTTGTAAAATACAAAATTTAAATATTAAAGCAAATAAAATAATAGCCCTTAATAAATTACAAGATCCAGGAAATATTGGTACTATTTTTCGACTTGCTAAATCTTTTGGATTTGATAGTGTTATAGTTGAAAATATAGATATATACAATGAAAAGATAATTCGTTCATCACAAGGAGCAATGTTTTCACTAAACATTATTAAGACAAATGATTTAAGCAAAGAACTGTCAGCCTTTAAAAAAGATGATTTTTTAGTTTATAGTACAACATTAAATAAAAATTCAATTAAGCTCAATGACATTGATTTTAGAAATAAAAAAATTATTGTTGTTTTGGGAAATGAAGGAAATGGTATAGACAAAAAAATTATTGACTTAAGTGATTTTAATGTTTATATTCCTATTGAATTTGAAAGCCTCAATGTTGCTTGCTGCGGCGCAATTATTATGAATAAAATTTATAATGGAGATAAATAATGCAAGTTTTTGATTGAGATTTTAAAGCAAAATTAAACATAAACGAACCTTTAACAATTTGTTTAGGTTCGTTTGAAACATTACATTTAGGGCACAATGAATTATTTAAAATAGCAAAAGATTTAAAGCAAAGTTATCCAGATCAAAAATTAGCAATAATGATGTTTAAAAATCCAGTTAAAAATTCTCAGGTATTAACAAAAAAAGCAATGCAACCAAAAACTAGATTATATACATTAGCAATGTTGGGATTTGATTATGTTTTTTTAGTTGATGTAAACCAAACGATATTGAATTGTAGTTATAGTGATTTTATTCAAGGATTAAAAATAAACAACGTTAAAAATGTTGTTTGTGGTCAAGATTTTAGATTTGGGTTCAATAGAGAGGGTAACATTGATAAATTAAAAAAATATTTCAATGTATATGTTGCCAATGAATTAAAGATAAATAAACAAAAAATTTCTTCAACAATAATCAATGAATTTATTGAAGAAGGAAACATAAATGGCTTGAACAAATTACTAATAGACAAATATTCAATAATTGTAAATTCTCAAAAATTTAATTTCAAATATCCAGAACATTTAAATCAATTAAAAGCAGGAATTTATATTGCCAATGTCGTTTGAAATAACATTGAATATCACGGCTTAATTTTTATAAATAAAAATCAATTTGACTCAAATAATGGCCTACAAGAAAACAACATAATTTATTTATTTGATATTGATTATTTTATTTCGAAATATCAAGAACTTTACATTGAATTTTTGCAATGCATTAGACATATAAATAATAATAGTGAAAATCAAATTTCAACAAATGATATTGAAATTGCAAAGAATTACTTCATTGCCAACAAAAAATAATATTAAACAAAAATAGCAAGTTTGTAACACTTGCTATTTTTTAACAAAATATATTATTTTAATTTAATCTTTGTTGTTCAAGTCAATATATTCAATATCGCCGTTTGAAATAACTTTAATTAACCCTAAATTATTTAAATATCCATATAGATCATTTAATTTTGAAATATTATTTTCTGTTCTTGCTATTAGTAATGCTTTTAAAATAGTATTAGTTAAATTGTTTTTTAAAATAGAACTTTTTGAATTGTTTAAAACTTGTTTTATTAAATCAAACATTTCCAACGAACTTATTTCTGATATTTTTCTATTGTCACAATCCTTTGGCATTCTAAAAGTATTTGGCTTTGAAACGTCATGATAAAAGATTTCGCCATCAGGATTTATTAATTTTTCTAACATTTTATTATTAACAAAATAATTTATAGAATTTTCACAAAGAAATTTATAAGTATATTCGTCTTTATTTAAAGGTAGGTTCACGAATATATCTTTTATCATTTTATTGATTTCTTTTTCTCTATAAGTATGGCCATAATTAAACATTTGTTTAACAATTTCATAGCCATTTAACAAAGGATTTCTTTTGTTTGACTTTTCTATAAAATTTAGTGTCTTACGATATGAGTAATCAAACGGAAGTCATTCTTCAAAATTAAAATTATCTCTTTTTTCAATAATTTCAAATTGACTATTTTCAATTGTTTTATATTCATTTTGCTTTACAACAAATTTTTTTTGCTTAATTTGATTTACTTTATCAACTATTTTTTCTAATTCTCTTCTTTTGTTTTTGAATCAATTTAAAGATCAAATTCTGTGAATTATTCAACCTCTTGACTCAAGTAGCATTTGTCTAATCGCATCTCTATCACGAGCGCTTTTTGATGAATGATATGTGGCGCCATCGCATTCAATACCTAAAATATATTTTGATGGATTTTTATCATCAACAATTGCTAAGTCTATTTTGAATTTTGAGCATCCCACTTGAGTATTTACTTTAAATCCAAGCTTCTTAAGTTCATCATAAACTTGGATTTCAAACTCACTATCAAATGTTTTAGTGTCATCTTGTAATATATCATCTGATTTGCTGTCGTATCCAAATTCTGCAAATTGTAAATATTGCTTTAAAAATCTTAGTCCATCATTTTTTAAATCGTCTACTTTTAAATCAGAATATGATATTGAAGATACAACAATAGTTGCTTCCTTGGCTCTACTTATTGCGACGTTTAGTCTCTTTTCACCGCCAACATTATTTAATGGTCCAAAGAATCTTGACAATCTTCCGTTTTTATCCCAACTATTTGTAATTCCTAAAATAATGATGTCTCTTTCATCACCTTGAACACTTTCAATGTTCTTAACAAAAATTTTATTTTCTGTGTCTAAATCCATATATTCAGGATTTTCATTCATAAATAAGAATAATTTGTGATCAATTAAGTTAGCTTGTTTTAAATTAAACGTTACAACCCCAAATGTTTTTGTATTGCCATAATAATCTTTTAATTGCTTTATTAATTCAATGATTCTATTTGCTTCAATTTCGTTTGTTTGATCTTCATAAATTCCATTAACCTTAATAAATGAAACGCCTTCAAAAGGAAGCAATTCCCCGTCTTTCTTGGCGGTTTTTTGATATGTTGGAAAAGTAATTAATTTATTATCATAAACGAACTTATTTGAAGGCAAAATTAATTCTTCAAATTTTGAACGATAGTGTCATTTTAATGTAACTTGTCTAAATGAGCTTTGCATTCTTTCAAGAAGTGAATCATAATCATTTTCTTCGTTGACTATTTCTTCGTCTTCGTTTGGTTCTTGCTCGCTAGACATAGTTTTAAAGAAATTAGTTGGTGGCAATTGATTTATATCACCGGCAATAATAATTTGTTTAGCCTTAATCATTGATATCAAGGCATTTTCTGTCGTTAATTGAGAAGCCTCATCAAAAATTACACTATCAAATGTAATGCTACTATCCGCTAAATATGTACTTAGCGAAGAAGGAGACATCATAAAACAAGGCTTAATATTTTGAATTAAATTTGGAATTTCTTTAAATAGTTTTCTAATCGGTTTTTTATTTCTAACTTTTGAATATTCCTTCTTTAAAATTTCTAATTCATCATTGTATATTGTTGAATTTAACGTAGGCAAATTGTTTGATAAATTTTCAATTATTTTGAATTTAGCAATTTCATTTAGCTTTAAACAAGAATCTTTAAAAACATTGATAATTTTTTCATATTCTCTATCATTTCAATCTGCTAAATCAATTGATAAATAGTAATCAATTAAAAGTTGATAAAATCTTTTATTAAAAATATTTTCAATACTATTTTGTTTTAAACAATCAATATCTAGCAATTCAATAGCATCCACAAAATCATTAGTAAATGGATTGTCTTTTAATTCTTGTCTTGATTTTATATAACTACAATATGCTTCGGGATATTCATCAGAATCAATTAAAAAATTTAGTTTTTTTATAAAATCATTAACTTTATAATTTGAATTTGAATAATCTACATATTTATTATTAAAGTAGTCATTGAATTTATCTTCAAAAGTCATTAAATTTTTATATGAATTTATGTAATTATTTGTTAATTGAATATGGCTTAATAAAAAGTTAAAAAAGCTAATTTTTAAATCTTGGTCAAATTTTAATTCACTTAATTGATTATTAATAATTTCGTATTTAGAAAATATATTTAGTTGTTGCCTTAAACTATTAAGATCCATTTCATTATAGTTTTCTTTAAATGAAAAAGGAATTTTATTTGTTAAAAGCGTAATTGCATTTTTAGCAAGTTGAATGTTATTTAATGCCACTAAATCTTTTAACAAGTTTTTGTAATTTAAATTAGAAGATATAAAATTAATTTTTAATTCTTTTTTTATTGTTCTATATCTCTTTGAAAAAATACGCTTAAAAATATTATTTCGAGATTTCACAAAATCTAAATTCTTATAAATTTCACTATTAAAAATATTTTGATTATATCTTGAAAGAATTCAGTCAATATCAGGTTGAATTTTTTCTTTTTGTAATAGTATTTCATTTAGGGTTTGTGTTGCATTTGTTTCATAATATGAAAAAATTTCTAAATATTTTGGATTTATATCTATATCTTGTATATTTGAAAAAATTTTAAAAAATGAATTAAATAATGAATAATTGCTATTTTTAATATATGGTTGATAATTGTTTGGAATTTCAAGATTTAAGAAATTGATTAGGCTTTGAATTTCATTTATTAAATGGCTAGAAATCTCTTTAATGTTTTCTAAATCTTGAAGCTTTACATCCAAAATCGAAAGCCCGTATCATAAACTTTCCTTAGCGTTAAAATTTATATTTTTATAGTTTAAATTAAACTTATTTAAACTACTTTGAATTTTAACAAACTGTTCATTATCCATTTTTTCAAAATCTCTAATATCAAAATTTATACCTTTTGTATTGGCTAGTAATTTTTGATAATCACCAATCATTAAATATAATGAGCGACCTAGTGGTTTTCTATTTGATAATATCTTTCTAACAAAATCATTCATTTTTTCTAATGAATCACTATATAAATAATCATTATTAGCAATAACTTCATCACTTACTTTTTTAAATTTTTGACCGTCTTGCAATGTTTCATACAAATCATTAGAAAAATCTTTTTTATTGCTGTTATTGCTATGAATAAGCAAAATAAAATTAGCAAATCCAAGATTTTGGATTGATTTATAAACAACATCCAAAGCAGCCTTTTTTTCAGCAACAAATAGAACCTTTTTATCTCTTGCAATTAATTCACTTATTATATTTGCTATAGTTTGAGATTTTCCAGTTCCTGGAGGACCTTGAATAATAAAACTATTTCCTTTAATTGCTGATTGAATAGCTGCTTCTTGCGAAGAATTAGTCATTAGGCAGTGATAATAGCCTTTAGGAGAAACTATATTATCAACTTCTTCTTCAGAATACAAATATTCAGAACTAAAAACATTGTTAATCCCTGTCATTGCATTCATAATTTCTGAATTAATGATATTATCTTTGTTTTTATCAACATCTAAATAAATGTCCATAGAAGAAAACCTAAAAGCAGACAATGAGGCTTCATCAACTATTTTTCAATTTTGTTTTATAGAATCATTGAACGAATTCATTTTTTGATTTATAAACTCAATGTATTGTGTATATTTATCATATAGAGAACCTTCAATATTATCAAATGCTTCAAATTTAATATCAAGTCCAAAATCATCATTTAATTTCTTTATCAATGAATTATTTACAACGAAATCATTGCTTTGAATAATTATTTTTTTATTTTGCAAGCCGTTTTCACTTTCAATTGAAACAGGTAAAAACAAAAGTGGGCTATAAACGTTTTTTTCTGTTTTATCCTTTCATTGAAGCCCACCAAAAGCCAAATATAGAATATTAATTCCTTGTTCTTCTAATAAAGTCTTTTGCTTTCTTTGCAATAACGACAATATTGCTAATAATTCTTTTTGAGAAGCAGAGGACAAAAATTCATTGCTTTCAACTTCAATGTCATCATCAGAATCTTGAGAACCAATAAATTTAAATTCACTTCTATTTTCAATTTTATCTAAAATTGATTTTATGTCTTCCTTTTCAATTGTTATAGTTGATGATTTATTTTTTTTATAATTTATCAATATATTTCTTAATGTTGTGTCTAATAGCTTATTCTTTAAAGACTCATATGCCAAAGTTTTTGTAATTAATTCTTTATTTTCCATTTCTAAATTATATTATTTTAATTTTAAAATAAATGTTGTTTAATATAAGATTTTATTAGCATATTTTAAAAAACAAAAATTTTAACGCTTGATGAATTAAAAATTGAAATTTTTACATTTATTGATCGGCATAATAATAAATTCATTAAAAAAATGATTCAAAAACACCCCAACAAATGTGGGATGTTTGTAAAAAAAACAAGACTCGTTTTATTTTGATGTGAGTCTTGTTTTTTACTTCTAGTTGCTTTTTTCTATTATTGAACCCATTTCTAAAAAACCTTGAGCATCTAATGCTGCTCCGCCAACTAAAAAACCATCCAAATCCTTAATTTCAAGCAATTTTTCAATATTTTTTTGTGAAACTGAACCACCATACAACACTGGAACTTGATTATTAGTAATTTTTTTAATTACAGATATTGCTTTTTCGATATGGTTATTTTGAGGAATTTTTCCATTTCCAATGCAATAAGTAGGTTCGTATGAAATAATTAAACGATTATAAACATCGACGTCTTCTAAAGCTTTTAATATTTGGTTTTTTATAATATCTTCAAAGACTTTATCGCTACAATTTTCTTGATCTTCCCCTATACACAAAATAGGAATAATGCTAGTTTTTTCAATTTGTTTAATTTTTAAATTAATCAATTGATCAGTTTCATTAAATAATTTTCTACGTTCACTATGTCCTATTAAAACAAAGTCAATGTTCAATTCAACTGCTGATCTAAATGAAACTTCCCCAGTGTATGCACCTTTTTCTTTATAAAAAATATTTTGAACTCCAAACAAGAAATTTCTTTTATTATATTGTAAAGAAAGAGACATTGCATCGAATGACAAAGCCGCTCCAACTATTACATTTTTAAAATTTTTAATTTTATTTAATGATTGTAGTTCATTTAAATAATTTTGACTTTCTAAATAAGATAGATTCATTTTTAAATTACCAATTAAATATTTCATATTAACCTCTTTTATTATCAAACTATTTTTAATGCTAAAAATGGAATCAATATAATCAATATAATTAATCCATATAATATTAATAAAAGAAATGATAAATTATTAATTGATTGATAGTTTCTTTGCTTTTCAGCTTTGAGTCTTTCACGTTCAAATTCTTGGCGTTCTTTAGCAATAATAACATCCTTTTGATGAATTGTCATTCCTGAATAAAATTTGTCTTTTGCGCGACGCATTATGATTCTATTTCTAATTAAATTAGCAATAGGATTGACAATTCATTTTCCCATTCCTTTTCTATATACAACAATTATAAAATTTATAGTAATTGCAAAAATCATTGATACAGTCAATGAATTTTGCCAAGAATGAACATATTGACCATCAACAGATTTTTTTGAATATCTAACTATAAAAATTAATGCAATATAAATCACTAATGAAACAAAAAAATAACTAATCGTTGCTTTATTTCAAGCATTCTTTCAATATGATATTGATTTCTTTTGATTTCTAAAATTTTTTTGTTTTAATGTCATAACATTATTTTATATTATTTTTTAACAATGCAAAACAAATTTAAAATATATCTGATTAATGATTTTAAAAATAAAAATTAAATATTATTTTGTTTCTTTACATACAAGGTAAATTAATTTATTTGATTAGTGAATTTATTTTTTTGTGATAAATTTCGTTAAATTCATTTTTATCATGCTCAACTTGTTTGAAATTTGTATATGAACTGTCTTTTTCGTTATTAACTAATTTATTAATATATGGGTTTATAAATTTTTCAATAAAATCTTGGCCATCTGGTTTTTGAAATATTTTATTTGAATTAATTTCTGCATCAGCCAAAATTTCTACAAACTCTCAAATAGACTCGTTTAATGCTAACTTTTGACTTATAGAATTAATTTTTTCATTTAAAGATTCTGCTAAATTATCATTTGCATGACACTTGTTAATTTCTTCAATAATTTGTCCTTTAAAATATTGATATTGATTTATTAATGGTATATATTTTTCTTTTAAATTGTTTTTTGCAATTTTATTGATAATATTTTCAATTATTTGGTCTTGGAATTTTTGTTGCAATGCCAAATTAATAAATTTAGTTTTTTGAATTCTAAGCGAGGTTATTTGCTTAATTTCTTGCAATTTATTTAAATAACTGGCAATATCTTTGCTATTAATATTATTCAATAATGCTTCTAGATTAATTGCCTTTAAAGTTAAAGATAAATTATCAGCAATTGATTTTAATTCGTTTCTTATTAATTTATCTATTTTAGAATCGTCTGCATCTAATTTAATTTTATTTTCAAGATTATTTAATTCGTTTACTAAATTATCAATTTTTGTTAAGTCTTTTAAAAGTGGTTGAATTTTAAGTATTTTTTGTTTAGCAACGTTTATTTTTTGTATTTTTTCATTTAAACTTTCTATTGATGATTCAGAGGATTCTTTATTAAAAGATTCCAATAATTTCTTGACATCCTGAACTTCTTTTAAGGTGTTAAATTGTTCAGCTCCTTCTATCAAAAAGTTATCTTTTAAATAACTAAAAAGATCAGTTTTTGCATCATTAAATTTTTTTAATAATTCTTCAGATTTTTGTTTATTTTCATCTTTAGTTTGTGTGGTTTCTATATTTTGTTTAGGTTGCTTAGGTTTTTTAAAACAAGAAGCTGCAACTAATGGGGTTATTAAAATAGTTGAAGATAACGGTAATATATAAAGTAATTTTTTTCTCATTTTTCTCCTATGAAGTGTGTAATATTTTTTTTAATTTTATATTCTTAAATTGTGCTATCTACTTGTATTTGTTATAAATCACGATTATTTTACCAAAATGTTCAATAAATTTAAGTAGTTTTATCAAAACAAATTATTTTCATAAACCTAAATAATTAAAAAAATAGTCTAATTTAGGCTATTTCTTCAATTAAAAATTTTTCACTATTTTATATTAATCAATTTCAAGTGGTACTTCGCTATCACCATCACTACTAAAAATTCCATTATTTGATTTATCAGGGCATATTACACTATAAGAAATTACCGGCAAATCCCTTAATCTTCCCTCTGATTTTAATTGTATTGAAATGGGGTGGCCAAACGGAATAAGTATCTTAGGCAATGTAACCTTAAAATATTTTACAAAATCTGCATCACTCAGTACATTTTTATATGCTTTATAAACTTGCGAATTTATTCCACTAGTTTGTTTATTTCTTATGTTGATTTCAATTTGTTCATCAATAGATTTATACTTATTGATTATTTCTTTTAAAATTCTTTGTAATTCTTTTGTTCCGCGTGCAGGGTCATTTAATATTTCTTTACCTTCATTAGTTAGAGTAAAACCAAATTGGTCTCTAATATATGACAATTGGCTTTGGCCTAATTGACCTTTTGCTCTCTTATTAGGATCTATAATGTCGCCAGATTGAGATTGTCCAGTCGAATCTTCACCTGTGGTTGTAGAATCTTGACTATCATTTGTTGTTTTGCATCCTGCTGCAACTAATGGCATTGCAACAACTGAAGTTGCAACGGCACTTAGTATTAATAAGCGAATTTTATTTCTCATATTATTCCCCCTGAATATAAATATATCGTTCATCTTCAATAACTTGTAAAAATTGGCTACTTCTTAATTTACATATTATACTATATTTTATAAATATAGTATCTGATTTTAAATTTGTATCACCATATCCAAGTTCTAATTTTAATGTATGATTTAATAAGTATTTCGAATCAGGCAATGAAATTTTAAAATATTTTAAAAATAACTCATCACTGAATATGTTTGACTTTTGATCATCTTTTTTGTATTTATCATTGAGTTTATCAATGATTTTGAATAAAAATTTCTTATTCTCTTGATTAAGCGGTATTTTGGATTCAAAATTAAAACTATCATAAATTGCTGCAAGCATAGTACTAGACATGTTTTGATTATTATTAGATAAAACATTATATTTAGTATGACATGGGCAATTAATTCCTTCAATAATCTTGGTTTTTAATAAATCATTTTTGGCTTTTAAATTAGAGGATTTTTGGCATGAAACTGCTGCAATTGTTGGAATTATTAAAGCTATTGGTAAACAAAATAGTGTAATCTTTTTATTCATTATTCTAAATCTAATTTTATTTCTTGATCTTTGGCATCATATGTTTTTACATCTTTGCAATATATGTCATAGATTAATGAAATATTGTTATTATTATCAATTTTAAAATTAATTATCAATTGGTGATTTACACCTAAATTCGGCTTAACCACTTTAAACAATTTTAAAAAGTTTGCATTATTAATTAACCTCAAACCTGCTAATTTATTATTTTCAGAAGGGCTAAGATTAATTTGCAATTCATAATCTTTACAAATCTTTTTTAGCTCATCTGCCAACGCCTTATTGCCATAATTATTTTTTAGCATTTTGCTCTGTTCAGTTAATTCGAAAACAAAATCATTTTTAATTGCTTCTAATTGAGAAGAATTAAAAATTCCACTTTTATTAGTGTAAATTTCATTTGCTTTTGCTGTATTTTCATCTGTTGAAACACAAGATGCTGAAATTATTGGCGAAGCTAAAACTAGAACTGCCCCTATAGATAATATTATTTTTTTCATATGTTTTTCCTATTCTATTACAATATCTTGCAAGTAAGACAACCCGTCATAACTAATTGGTTTATTAATAAATTTTTGGTATATTACTTTTGAAAAACCAACAGTTTTAATGATATTTTCAAATGAAAAGGTATAAGAAAGTAAACTGTTAATATCATTAATTATATTTAATTGGTCTTGGTAAGAGATATTTTCTAAGTATTTTAATATAATTGATTTTATTATAAAGAAATTTTTGTTGTAATCTTCTAAATTTTTGTTAAAAATTTCATTATTTGTAATTAGTTTTGTTTTATTTAATTCATTTAATATTTTTTTGGTATTTTCTAGAATAAAACTATTGCTTTTAGTTAAGCTAGAAATTTTATTAAATATTAAAAACAATTTATAGTTTTTAGCAATAGCTGTTGCTATAAATTCTTTAGTATTTGATTTTAAAAGTTCAAAGCTATTATTGCAATATATTGAAGCATCTTGATTAAAATACAAGTCATATTTTGCTAAATCATCAATAACTACAAAATTTGGCTTTAAATGTTTATTTACTGCTTTAAATATTGTTTTGATATGTTCCAAAACATTTTGCCTTTGGTTATTAAATTGCAAATTCAAAAAATTTAGTGGAATATCAAATTCAATATCAGCAGCATCTGGATGTTGTTCATAAAACTTTTTGATTATATCATTTAGTTGTTTAGATATTTCTTGAAAATAAGCTGATTTTAATTTGTAATCAAGATTGCTTGATTGAAGATCGTTTAATATTTTTTTGTTTGCAAGATAATAACTATTGTCAATAAAGTCAAGACTATTTTTATGAACACTTTTTGGAATCAATGGCTTAAAAATGTTTACCATAGAATCTTTTAAAACATGATAATTAATTCCACTATCAAAGCTTGAAATATCTAAATCGCTTGGCGCTTGAGATGTTCAAATATCACCTTGGTTAGCCAACATTCCATATTGATTAATCAATTGGTGAATCAATAATAAAAAAGCAATATTTTTAGATTTTAATATTTTATTTATATTCGTATTTTTATTTAATTGGTTTACTTTTAAACCGTATGCTAACATTGAAAAACTATCATTAAAAAATTTTTTATTTGCAGTCGGCATTCAATTAATGGTAATATTATTACTCAACTTATTTTTAGAAACATTACGAGTATAACTTATATTAAAATCATTATATTTATGCAAAATTTCTTGTTGTTGATTTATGTCAAGATCGTCAAAATTCAATGTAGTTACAATATTTTGCAAAAATGCATGATACATTTGCATACCAAAAGTATTTTTTGATATTGGCAATGTATTAAATTGAAAATCAATTTTTGAAATTGATTTTTTATTTTTATCGTAAAAGAAATTCTTTAATAATTTTATTTCATCTACATTTACTTCATTAATAAAATAATAAGATGCATAATATCTATCTTTAAAAGTTTTATCATATTCATAAAATCAGTCAAGGTTTGTTAAAAAATCTTTATATTTTTCATTAATGATTTCATAAGGCATTGCATCAATGTAGTTTGAATGTATAAACAAATTGTTAAATAAATCAACTCAATCATGTTCAATATTATCAGCACTACAAATACTTAAATTTTTGTCGTTAAAGTCTAGTAATTTATAAACATCAACGTTGTAACTTTTTAAATATTCAAATAAATCAAAATTATTCAATTGATTTTGCTTTAAAAATTCTTGTTCTTTATCTTTGTTAAGTGTTATATTATTTTTTAATGCTCATGATCTTCTAAAATTTTTATTTAAAAGCAGCATTCTTAAAAGACCTAATTTGAAATCCTTAACATTTACTCAATGAACTTTATTGTTTATATTTCTAATTCCTTGCCAATTAATTCAATAATTTTTTCTTTGATTTATACTGATTGAAATATTTTTTGCTTTTCTTAGAGATTCAATGAATTCTTTGCTATTTATAGACATTGAATTTTTTGAAGGAACGAACAAATATGGATTATTAAAACCATTGCCTTTGTCTTTGCTGGGGTAATATTTGTTGGGATCTTGCTTATTCAATTCAATGTTTTGATAGTCAACATTTAGTTTATCATCACTATTGAAAAGTTTATCATTTATTTGAATACTATTACCATATTCAAATTTATAACTTAATTCAGTCGGGCGCAAAACTATATTATCCCTAAAGTCTATTTTAGGTTGATTTTTTGAATAAATTCTAAGAACTTTGGCTCCTGTCATTAAATTTATTTCTTTTAAATTTTGGTTTTTAAAATTTGGTATTGAACTGCCATATTCTAAATAAGGATTTGCAATATTAAGCTTTTGAATATATTTTTTCTTGTCTTCATTAAAATTATTAAATTTACAAGATATTGATATCGCAGATGTCATTGTAAATAATGCTAACATTGAACTTAAAATAAGAATGTTTTTTTTCATTTTTACCACCTTAAAATGCCGTTGCTGTATGAGTGTGAAAACATAAAATAACTATAGTTAATACTAGTCCTAAACTAAATAAAGCAAATGAAGTATATAAAACTTTTGGGGATTGATTTCGAACATGGATAAATTCCGGAACCAATTCAAATATGGACATAAATGTAATAATTCCTCCACCTAATGCCAAAAACGAAGGGATTATTCAAAACACTTTACTATGTTCAATATATTGACCAAGCAATCCGCCAATTAACATAAATGGGATAATTAATAAAAATGTTAAGAAGTTGTATAGTATGGCTTTTGATTTCTTTTCTCCATATTGAATTTGACGATAATAAACAACTAAAATTTCAAGTAATATATGAATATTAAATGTTATTATTAAGGGAATATTTGCTTTATATGTTCCACCAATTGATGTTAATGTATAAACATTATATCCCAAAAATAGTCCATCAATAATTCTATGACTCATTAGCATTAAAATAGCGCCTCATGCTGCTCTTGGATTGTCGAAATCTTTTATTGAAAATAAGTGGTCCGAGTGATTGTGTTTATCGTGATCTGCATGAACGTCTGTTTTTAAATACTTTACGAAAATGAACCTTCCTAGAATAACTAAAATTAATCCCAATAATGCTGATAAACCAATTATTGCAGCTTGGATTCAAGGAATATTAGTTTCAGAATAAACTCTTAATCCATGTAAATAGCTATTAGATCAATCAAAACTTTCTTTAAAGAAACCTACAGTGCCAATCATTAGAAACATTCCCGTAGCAAATGCATAAATATAAATATTTTTCTTATCAGTAATTTTTAATTTAAATATAGAAATTACGCCCAAAACTAATGAAGGTACGCCCAATAATATTAAAATATAGATTATTAGATTTACAAATGTTGCTAAAGTTATATTTCCGTTAAATGCACTTGTTCCTATAAACATGAAATTCCTTTCTATTTCGTTTATTATACAATAATTTTTAAACAACTATACAATAAAAAATGATAAAATTGTTATATATTATGAAAAAAAAGTTTTATTTTAATTCTATTTTAATTGCTAGCTCACTTAGCTTTATGCCTTTAATTATTTGTAGTTGTCAAACTAAAAAAGATAACTATATTATAGAGGGTAGCAATGCTTATATAGATATTTCAAAAATTTCTAGAGTTTTTTTGCATAGGTTAAGCATAGGTCAGATTGCTGCATTACACAACATGTATAAGATTTTTTATTACTATGATAATTCTAATAAAAAGCAATATTATGACTCTGCATATGTAAATGGCCAAAAGCTATATTTAAGAAATGCAAATAAAAATGTTGAATATAAATTAAATTTTCCAATTAAACCTTCTTGGAAACAAGAAATATCAAAATTTGACAATATTAATATTGTAAAAAGCAATAAACCAAGCGATATTAATAATTTTTTAAACCAATATAGTTTTGATGAAGTAGATTCTGCAGGCGATGTTAATGACGAATGATATAGCATATTAGGCGATAAATGAAAAAAAGACTTTAACAGAAATAATGATCCTTATTTTGAAGATATGCAAACTATTATTTTTAGATTAATTCAAGATACAGAATTAAATTATTCAATAATGAAATCTAAATATTTAATAAATTCTAAAAGAGAAAATGTAATTACACAAAATTTATTTAAAAACAAATACATTCAAGCTAGTTCATGACTAAATGATGAGGAACATGAGCAACATCGTCAATGATTCAAAAGATTTTTAGTAATATATTTGAATAAATTTAATGTCAATGTCGGGGATGTAGATATAGATTGGAAAAATGCAAAAATTGTAAGGTCTTTTTCTGGAACCACTGATTATATAAAATTTAAATTTAAAGATATTAAAGATTGAAATGGAAAATCATTATTAAGAAATGATAAAAAAAATATTGAATATTACATAAATGGTTTTAGAACATATGCAACTAATCAAAAATTCGGTGTCGGCAATCAAGGTTTAAAAGAAGAACTACCATTATTCAATGATTACGTACCAAATCCACTTCTTGAAATTGATGGCATGAAATTTATGAATATTATTGATAATATTAACTATTTTGTTAAAGGCGCAACTGATATTAATTATTGAAATGCAAAAGGATTAATGTATCTTTTTCAAAGCTTTAAAAATGAACCTGGTTTCTTTAAATTAGCAGTTCCCGAATATAAAAAAAATGAAGATAAGGAATATAAAATTATTGATTTTGAATATACCCCATATTTTAAAACAAATCAAATTTTTAAAGCAATAGTAAGGGTATTTAAACTAAATGGTACATATAAAGATTATGTTTTAATATCTTCTAATTTTGATGATCACGGTCACAGATTAAAAGGTTTAATTACTAAAAATGCTTTGCCAAATGAACTTAAAGCTTCTGATATATACTCAATTAGAGCAGATAGTGAGCCAATTAAACAAGGAATCAAATTGGATGATTTCATTGATAAGAATAATAAAAATAGTGTTTTTAGATATATGTTAAATGAATCTGCAAAAAAACTAGAAAATATTTTTGAATATTGAAACAATAATAATAAGCAAAATTATGAAGTTGCTAATTTATTGCTTGCCAAAGATCCATTCCAATTAAAATTATTAGCTTCTTATTTAAATAACTACTTATTAGCATATGCACTTGAAAATAAGGCAGGTGAAGTATTTAGTGGAATTAAACGCATCGATCTTAACATAGTTAAATTACCAAATCAACCAATTGGAAGATTATATTTAAAGATGGATTTTGTTTCATATGCCAATAACCAAGATTTCAAATTCAAATCAAAAAATGAAAAAATTGTAAAAAGTGTTTATTTATATTGAAATGGATTTAAAGGATATGACCAACAAAAATATGGAAATGAATATTTTACCATTGATAAAATTATAGACGGAGATAATTAAACATGAAAAAATCAATAAAATTGCTATCCTTGCTTTGTACTTCTACAATAATTTCATTGCCTGCTATTTCTTGCACTATCAAAGTAAATCACGATAACGAAGACAAGGAAAATTTAGATTTTTCAAGTGATTTTAGTGGCAAAAAGCAAAAAAATGAATTCAATTTCAAAAATAAGATTTCTCAAAATATTTATGATTTGTATCAAAATAAAATTGCTATTTTATTTAACGATGTAAAAACTAATTACCGGGGATATAAAAAAATATTTGGATCTTTAACAAGAAACTTAAACACTCTAAGGGCTAAGATTAATAGCTTAAGATACGAACAAAGTTTAAAAGAAAACAAAAAAATATTAAATGATTTTTACAATAATTGATTAGAAATAAAATCATCAATGCTAAAAAATAATCCATTTGCATTATATTTATTCAAATATAGTTTAATTTTTTCTGATGTGGATGCTGTATTAGCTGATACAAATCTTGTTTTTGAAACTTCTGAATTTTTAAAATATTTAAAAACAATTGATGACAGACTTGAAGGTAAAGACATTGATTTAGGTCAAACTCAACATGCATTACAAGCGGTTTGAGATTTTATTAAAGCACATATATTCAATCCAAATAAATTAACCAAGAGAGAAATGCTCGATAAAATTAACATTGAGGATGATAAAAACTCGCATAATCACTCACATGCAATCATAAATTTAACATATGAAATGGGTTTATGGCATGAAATGCTGAAAAAAAATAAACTTCAAAATTACGAAGAATTTCAAAAAGAATATAAAATTGCTAAAAAACACATAATCGATAATATAAACAATATTGATGTAAATAATAATTATTTAGCATTAAAAAAAGTCCTAGATAAAAATTGAAGTTTGGAAAATTCTTATTACAACTTATTAAATGATTCATTTTTTAGTAGAGCAAAAGAAATTTTAAAAATACTAAAAGAAAATTTGATAAGTATTGCTAAAATTGTGGGTTTAACTGATAAAATCAATTTAGACTAAATCTTTAAATCATAATTCCTTTTTAAACTAAAAATAAACGTAACTTGTTATAAACCTGGACAAAAAAATTAGACTCGTAATGAAAAAATGCGAGTCTTTATTTTTATTATTGAAAGGTTAAATTATTATGGGAGACAATTAAGAGAAGATGAATGATTATCAATATTTTTTTGATATGAACAATATTTAAATCACGATATTTCTAAAGGATTTTTATCATATAAATATTGTGAAATTAGTAATGGAAGGCAACTAAATAAATATAGTTTAAAACTAATTAAAGCCAAATATAAACTCTATAATTTAGGTATGAATATAAAATCACAAACAGGCAATGCCCAAAAAAGCTAAAGGCTCAGGTCGTCCAAGAAAAAGAGATATTGAACAAGAGAACAAACTTCGTGAAAAAACTTTAAAAGAAATGTCAAGAGAACAACTCGAATATCTCATTACTGATATTTTTTATGAAGAAATTTTAAAACGCAACAATAGCAAAAATTTAGAAGAAGTTGTTGAAAAAATTAAAAAGAAGCATAATTTTCAATATCAATAAAGCAAATTATCACTATTTTAGGGATTTAAAAATCTACTTTTTATTATAGGATTAAAAAATTTAAATTGCCAGCCAAAAACAAGGCAATTCAATATGAAAAACAAATAAAAAAAAAGGGGGGGGGGGCTTTTATTGAATGCAAAGGAAGATTTAGAAGGGAAAGACTTTCACTTTTTTTAAAGAAAAAGTACCAAATTGATATAAACCCAAGGACTCTGGGCAGAATTATGAATAAATTGGGTTTGCTATGCAAAATAAGAAGAAGCAAAAGAAAAAGAGAAATAAAGAATGTTAATGCAACATTCAAAAACTTAGCCAATAGAGATTATGATGGCCAAATAAATGATATATATGTTACCGATGTGACATATATTCCTTCACCCACTGATATTATAGAAAATCATGTATATTTATCGGTTTTATTGCATCATAAAACAAAGAAAATTATGAGCTGGAATTTATCAAAATATAATGATACTAATCTTGTTTTAAAACATTTTAAACTAACTAAATTTCCTAAAAAATTCATTGTACATTCAGACTACGGATATCAATATTCCTCTAAGGAATTCCTTGATTACATAGATTCAATTAATGGCACTATTTCTATGTCAAGAGTTGGAAATTCTTTGGATAATAGAGAAGTAGAATATTTCTTTTCTATTCTAAAAACTGAAATATTTTCAAATATTTTCAAATTTTTTCAAAAAAGCAAAAAGTTTAACATTTAATGAATTGCAAACAGAAATAGACAAATTTATTAATTGATATAATAATGAAAGATTTATCAAAAAATTTGATTTAAAAACACCCCAACAAATGCGGGATGTTTATATGAACAATAAATCTATTTGAGTCTAAATTTTTTGTCCTAGTTTATTTATAGGCTTTTTTTATAAGTTTATATTATTTTTTATCTAATGTTCTAAATCTTAATGATAAGCAGCTAAGTCCACCATCAAGTTTTCTATATTCACTTGTATCACAAGTAATAACTCTATAAATACCTAAATCTTGAATAGCTTTTAAAGTTTTTGGATATCCTTCAGGTACTATAACAGTTTCATTAACTCAAATACAGTTTGCGGCATATGCTTCATCGGCAGGAACTACAATTTTGTTAAATGAATCAAATGTAGGATAGTTAACAAATTCACCTGCAATTAATAAGTTATTATGTTCAAGATAGTTAACTCCTGTTTTAAGGTGCAACATTTCTGTCATTGGTACTGGAACTAACTTCTTACCAATTTTTGCTAATATATTGTGGAATTGTCTAATTCCGTCACGATTAGTTCTTTCGGACATACCAATGTAGTATGTATCTCCAACCATCATAACATCTCCCCCGTCAACAGTTCCGGGAGCTTCAATTTTTGCAATGTGTCCATCATCAAAGTATTTCTTTAATGCAGGAAGCATTTCAACTTTTTCTCCATTACGTGTTTTAGCACCTGGATTAGTTAAAATAGCTAGTTCACCTGGAATAATAACAGCAGCATCTTCTACGAAGCATGAGTCTGGATATTTTTCATTTAAAGGTAAAACGGTTATATCAACACCACAAGTCTTTAATTTTTCAATATAATCTTTGTGTTGTTTTTTAGCAAGTTCATAATCTGGATGTAAGCCAGCTGATGAAATACCATTTACCATAGCAGCACATGGGGTTTTAACAATAATACTATTAAATTTATTTTTTAAAGGTTTTTCACATGATTTGCAGCAATCTTTGCAATCATTACATTTTTTACAATTACACATGTTTTTTCTCCTTAAATATAAAATTGTCGTATATATAACTCATAATTTTACTATTGCTATACATTTATAATTATGCCATTTTATGGTGAAGTAGAAATTTAAAATATTGAATTTACATAAAATTGTTTGTTCCTAAACAAATTGATAATCTTTTACTAAAAAAATTATAAGTTAATTTAGTAAGAGATTTTTTATATTTTTTGCCAAATGAAATCGTTTATTTTTTGGTTAATTTCTATTAATTCATTATAAATTTTTGTTTGATTTTTAATAATTTCAATTGTTGTTTTATTTTGTTGATCTAAATACTTTTGATTTAGCTCAAAAAGTTTTTGATATCCCTTGTTGGTTTTTTCTTTTTCAGCTCACAAGGCAACTAATGCACCACTTTTGGCATCAATTGCATGATAAATATGAATTTTTTCATTGGTTACAAAAAATCTATCTTGGCAAGCATCTACTTCAATAATTTCACCAAATTTATAATTTTGGTTTTTATTTGAATATATTGACAATTTTTTAGTGTTTTCAATCAATAAAATATTCGCCTTATAAATTAATTTTTCACTATTGCTAATTTTGCTGTCATTAATTTTCAAATTTGAAAAATATTTTTTGCAATTCTTTTAATTTTATGGGTAGCATAAGGACTGCAAAAACCATGCTTTAATCAAGCATTATATAAATACGATAACGAAATTTTTGACTTTATTTCTTCACTAAGGTCTTCATAAAAATCTTTAACAGTAATAAATTTTTTAGTAACATTTGAATTTATTTTTTGATTTTCAATTTGATATTTCAAAAATAAGTCTTTTGCAAAATCGTTTTCTATTATTTTTGCATTTTGATTATTAAAGTTTTTATGTCTAATATTTACTTTAATTTTGCTACCTTGCATATTATCTTTAATAATTTTTTTATATCTACAAATTGTAGGAATTGATAACATTGTTTTTTCTTGCAAATTTTTGGAAATAAAATCTAAATTTTTAATCTTTTTAATTTCATATTCTGAAATAATTGAACCATTAATTTTTGCCATAAATAATACTCTCTTATTTAATTTACAATTTTAAGTAAAAGATTATCAATTTGTTTAAGAATAGACATTTCTTTGTGAAAGCGAACAAATCAGTTGCGAAAACGAATTTTTTATGATATAATTTTACCAAGGAGGATTGAAGTATGGCACTAAGTTATAATAAATTGTGGAAACTTCTGATTGATAAAAATATTAATAAAACTGACCTAAGGAATATGACGGGAATTAGCCAATCTACCATGGCTAAACTCAATAAAGGTGAAAATGTGAATACTGAAGTACTTGAAAGAATCTGCATTGCTCTCGATTGCCAAATTTCTGAAATAGTTGAAGTAGTAAATAAAGAATGTGAGGAAAAAAATAATGAATAATTATAAAAGATTCTATGACGAAAAATGGAATTTTAAAGAAGGCAGTATGAAGGATTTGGTTCACGGTTTACATCCATATCCTGCAATGATGATGCCATTGATATGTAGAGAGATGTTAAATCAATATGGAAAGGGAAGCGAGACCGTATTTTTAGATCCTTATGTTGGCTCTGGCACAACCTTGGTTGAAGCACAATATTATGGTGCAAAAAAAGCGTATGGAGTTGATTTGAATCCATTAGCCATCTTAATTTCGAGGACAAAAACAGAAGAAATAGATTTAAAACAAATACGAGTTTATATGGATTCTTTCGATGAAACGATTAGTAATATTAGTGATGATGTTTCCGCACCTGAATTTTCTATTAGAGACTCATGGTTCAACAATAAAACGATAAGAGATTTAGCTAAAATCACCGCTTTTGTAAATTCAATAAAAGATTCTACAATAAATGATTTTTTCAAAATTTCTTTATCAGAAGTGATTAGAACGTCATCTGAAACAAGAAATGGCGAATTTAAGCTTTATAGAATGTCAAAAAAAGCGTTGGAAAAATTCAATCCTGAACCAATAAGAATATATAAGGAAGTGATTGAACGTAACTATGGAATACTAGAATCAATTAATTATAAGAGAAATACTGATATTATATTAATTAATGAATCAACTCTAAATTTAGAAAAGCACAATGAACTTAATCAAAAAGTTGATATTATAGTAACTTCGCCACCTTATGGTGATAGTCATACAACTGTTGCTTATGGACAATTCTCAAGATTGGCAAATGAATGGCTTGGATTTGATGGTGCCGGTTCCTTGGATAAAAGATTATTAGGAGGAAAAAAAGTCCAAAATAAGACTTTTGATATCCCAGAGCTAGATACGGCAATTGAAAAAATCAATGAAGCTGATAAAAAGTTTAATCGTTGTAGAGCTTGGGAAGTTGTTGCTTTTTATTCTGATTATGAAGACTCCATAAAAGCAGTTGATAAAACTGTGAAAAATGGAGGATACGTTGTTTATGTTGTTGGAAATAGAAGAGTAAGAAATATCGAGCTTCCTTTAGATGTAATAACCTATAAAATGTTTGAAAAGTTAGGATATTACCACGTAGTGACACATGTAAGAGACATTTTAAATAAAAGAATGCCATCGAAAGCTTCTCCGTCAAATGAAGCAGGGGAACAGATTCCAACAATGACAAGCGAATATATCGTTGTAATGAAAAAGAATTAATAAAAAAGTCTATTCAAGCATATAAGCTATACTTGGTAGACTTTTTTCATATCGTTTCTTAATGTTTATTAAATTTCGCTTTTTAATTCAAAAAGCTGATCCGTGAGCATGTTTTTTGCCTTCATTTTTTTCTGACCTATAAAAGCCAAATCTAAATTTAATAATTATTTTGCCATTCTTTTACGAATTAATAACATTATCAAAAGTAATGTTTTCTATAAAAGTAAGGCTATCAAACCAAATAAAGTGTTTACCATTATCGTCTTTCTCTTTTTTATCAACGATTGCTATATTCTTTATTTTGCTTGCTATGATTTCTTTAATTTTCGTGTATGAAAAAATTCTTTTTTACCGGTTTTCTCATCTTCGACGATATTACCAGAAGAATTTTTACTAATAGAAAATTTTTTTGTTTTCATTGCCAAATTTAAGTTTAAAGGCAAATGTTTTAATTTTGTTATATGTCCATTTATCAGCTGAAGCTTTGACATATAAAAGATTATATTTTTCCCCATTATCACGGTAACCGTTATTTTTTCGGATAAAACTGTTAATGTCTCTTGAGGATGATAGAACTTTGAAGATAGTGAATTTTAGATTTTGAATTAATTCGATGAAATTTTAGTTAAATAACACCATAATCAGCCACTTGTTAATTGTCTTCAGCTTTTCCTGCATAGTATTATAAAGCGTTGCCGATGCATTCATCGCCAGAAAGATTTGTTTCAAGCCATTTTTTTTCTTAAATTTTTTGAATTCACATTTTAATTTTGCCTTATCTTTTTTTATTCTTTCATTAATCTATCTCCAAAAGATCAACATATTTCAACAAAGATTCTAATTTAATTCTAAAAGCAGTGCCATGGTCGTGTGTTTTTCCTTTGTTTTTACCAGATGCATATACTCCTATTCTTATATCGATCATTAAATCACCACACTCTATAGCTTTTAACATTTTTTCTAAAGATAGACCTTGAATAATCAACATTCTTGTGTATCGAACAAAGTGCATTCCGTTTTCATCTTTTTCATCGCCATAAGTAATTGCGATTTTTCCTAATTTTTTATCTAAAGCATTTGATAATACTGAAAAATCCCAGTTTGTGTTTTTACCTTCGTTTTTACCTTCTAAAACTATTCCATTGTTATTTACAATTGAAAGCCAAAGCTTTTTCTCGGTTCTATCAACAATAACTTTAAATCGATGTCCACCTCTATGGGTATTATATTTGTCGCCACCCACAGTAGTGTTTAATATTTGTTTTCCTGAATCTGGTTCAATAACACCAAATGTTTCTCTTAAGTAAGTGTTAACTCCTTTTGGATGGGAAGGAGATTTTGAAAACAGAGAAACCATAGAAGAAGTAATACTTCTATGCGTTTTCAATTCAATGCCATGAAAATCAGCTTCATTTTTGTTGTTTTCTTTAACACCTAACAAATCTTCGAAAGCATTGCCTAAGCATTGATCACCGTGCCTAGTTGTCTCAATCCATTCTCGACTTTTTATATCCTTAAAAAAATTTATTAATTCAATTTCATAATTATCTGTCATTTTTTTACTCCTTAAACAATTCTGTTTTTTACTTTAAAAGATATGGCTTATCAATTTCTATTATTCGAAATCCTGTACCTTGATCATGAGTTTGTCCATCTTTTATTCAACCATGATATTGTCCAATTCTTAAATCCACATAGATTTGACATTTTTCTAGTAGTAATTCAACAAAAGAATAGTAGTCAAAACCAGACATCTCATATGCTTCTATATATCTGAATTCTCCATAAATTCCAGTTCCTATTGATTCAGCCATAGCATAAACAAACTTATTTCTATATTTTTTAATTCTTTTCTAGTCTAGAAAGTATGTTCATTGTCATATTTGTCAACTATAGAAATTCTATCTGCATAACATTTTATTATGAGTGTGTGTCCTGTATCTGCTATTTGTGTATAGTGGTTAGCTGATAAAGCGGATTATTATACGATCGGAAAAGGTATCCAAAAGTCTCTCTCAAATGATTTACAGCTCCTAAAGGCTGTGGAGTTTTTGCAAATAGTAAGAGCATACTGTTGGATTCAACTCTGCACGATTTTAATTCATATTCACAAAAATCAGGCTCATCATAGTTGTTTTCAATAACTCCAAGCAAATCTTCTAGTGTTTTTCCTACGCCCGTAGAACCTGAACGATGTGTCAGAATCCATCCGTATTCTTTTATTTTTTGAAACTCATAAATAAAATTTTCTAATATATATCATTTAGCTTCCTCTTTATTTCCTATGTATTCCATTATATCGCCAAAATCACACTCAAGATGTTTACAGATTTGCATAAAACATCAGTTGTGACATTGGCACCTTTTGTTAATTTTGCCATAGAAGAAGTGCTAATTCCGGTTTTCTCAATTAATTTTTTCTTATTAATATTTTTATCAATAAGAAGTTTCCATAATTTCTTATAACTTACCATTTAATACCTCATGTTTAGTTTTGATACACATCTTTTAAGAAGCCATATCTACCGCGTGTTCTATTCTTAGTAAATTCTATAACTTTGTTTGTTTCACCAGAAACTTCAGGTAGTTCATGATTATCTGTGTTTTCGATAATGATAATCTGATCATCTTCGTAGTTTTCAAATATATATTTGAAGAACCCTTTCCTTAAATCGTCTTCATTGTTCTCGTCACTTGTCTCTGTCATTAAGCCGTGAAGAGGGGAATCAAGAAAGTAGAAGTGCGGATTGATCGCACGTGATCGTTCATATATTTTCTCAGTGCTAGTACAATAATTGAATTGAGTATTGCAGCATAGCCCTTGCTTCGATTAGGTTTTGGATTATCATTTACTATCATATCAAAAGCCGCTATATCAAATTTAACGGTTTCTACAGGTGTGAAGTTCATTGCTATTAAAATATCTCTGTAATTATTTGCAAGTTTAACAGCAAAATCGCTAGCGAATAGGTTCCTTGGCTTGAAGTTAATTTTTGCTTCAACCTTATCATATTCAGTAAAGTCGGTTCCATATTCCTTTTCCATGTCTCCATACATAGCTAACGCTTGTTGATACGACACGATATCTTGATAATTTTTAAGTTTATCCTGTAGCTCGGTTTGAGATGGAAGTAGAGTGTTATTTAGTTCTGTTCAAGCCTATTATTCTTATTTTCAAGCTCACTAATGGTTGCTTCTAGTTCTGCTTTCTCTTCTTTAAGATCTTTCTTAGATTCATTTAATCCGTTTAAGTCATTAATTGTGCGATTCAATTCACCTCTAGAAGCCGAAATATATGATTCGTGGCTATGTTTAGTGGTTACGGTTCCGTCACAGAAAGGGAATTTAGTTGTTTTTGGAATTCCTGCAATAATTTTTGCATTATCAACAATGAAACTTAATCTTTTTATATTTAATGTGTATTGAGACTCTAGAATTTCAAATCTGCTTAATGAGATTAAGACTCGCTTTGTCTTTGCTTAGCTGAAAGAAGAAGTGGACCTATTTCATCTTGTTGAGAGGTAATGGAAGAAATTCTGTCATTAATTTCCTTCAACTTGAATGTTACTTCATTAAATAATCCCTCTATATCTTCAGGCTTAAGATTTTTATTTTATTCATTCCAAAAGTAAAATGCGCTTAGAAGAAAATCTAGATGCATTTTTACCGCCTTTATATAAAAATTAAGAATAAATTTTTAAATTTGCTATTGTGTGCATTCTAAAACATTCTTCTTAAGAAGTTTATTCATTATCAAAGTCAAAGTCCTTAAGAATCAAATTCCTGAGGACTTGGCAATAAAAAGCCGTTTGGCTTTTTATTTTTTAAATTCTTTTTTAACGTACTTAGAGTTAAATAAATAAAGCAAACTTCCTCAATCTAAATCAAATTTAAGTATGTCCCCACATTTATAATTAATTTTAGAATCGTTAGCATTAATTATTAAATGGTCTGAAGATCCACCTAATATTTTTAAATCTTTATCATAAGGTATCATCGCATCAAACATCGTGTCTAATTTGCCGATTGCACAAATTATTCTTTTAATATTTCCAATGTCTTCAAAATAAGGCTTATTTCCAAACGCATCAATTCCACATTCACCAATTGGCAATGAACTTTTGTAATCAATTTCTACAATTTCAGCTTCGCAACTAAATGCGTCTCGATACATACCTTTTATAGTTTTCCTATACATATCTTCTGTACCAAAAATCATTGCAAATCCCATTCTTAAGAAATTAATTTCTTTAGGCATACGATTTTCTCAAACTAGGTGCAAGCTTAGTGAATTTCCGCCAGACATATGAGTTAATTTAATTTTTAGTTCATCTTCAATTTTGTGTTGAATATTTACAAATAATTTCATTGATTCATCTGAGGGAACTCTAGCGCCATAGCAACCGAAGTTACAGCCTAACCCAATTAATTTAACACCTTTTAGTTCATGTATTTTTTTAGCAGTGCTCAAAGCATCTTCAGGTAAAACACCTTCTCTTCTATCACCTAAATCTATCATTAAAACTATTTCATGTGGTTTTAAATTATTTTTAAGACAATATTCACTAATTTTTTCTATTACTTGAATATCCCCATTTAAACTTGAATCACAAATTTTTACAACTTCAGAAATTTCTGAAATCATTGGTATTCTTAATAATTGTTTTTTAACATCTTTAAATTTATTAAAGATAGTAAAGTTTTCAAGTCTAGAATCACCAAAATACCTAATTCCTGCTTCATAATATAGTTCAGCCATTCTTTGATTTCCGCAAAAACCTTTAGTAACGGCAAGAACTTCTATATTGTGTTTTTTACACATTTCAATAGCAACTTTTATATTATTTTTAAATTTTTTCTCATCAATAATTATTTTAGGATATGACATTTATTTACCTCTCAAATATTATTTATGTAATGATATTTTACAATAAATAGCTTTAATTATTAATTAGTTAGCTCTATAATTTTAAAATAAAAAGGATGTTTATAAACCTGGACAAAAAAATTAGACTCGTAATGAAAAAAAATGCGAGTCTTTATTTTTTTAAATTATTAATCTATTTAATTTTTGCTCTTTTGATCACTTAATGGATCAAAAGCAACGTAAGGATATTTATCTTTTAATGTAGATCATATGTATGAAGTATCAATGTTTTTGTTGGCCTTTTGAATAGCTTCACATTGTTGATATGAATTTATTGCATCATATATTGCTTTATATGTTGTCGGGTTGTTTTTTTCTCATGCAAAATATTCTTTGATAACAACTTTATCAATTTTGTTTTCAGTATAATTTGAAAAAAAGCAATGAACTATTTCATGTAGCAATGAATAAAATATATATGCTTCAGAATTAAACATATCAGAAAGGAAAATATACCTTTTTGCTCCCTTTTTCAAAGCAATAGCCCTTATCAAACTATTTTCAATAAAAGGCATCGTTACTAGATTAATTCCTTTGCTTAATAAAACTCTTTTAATTGTTTCCATTTTTTCTTCAAATGAACAATGTGTATTAGACATGATCGTTAAAATCTTATGATAAAGTGAATTAAATGAAGATTTTCTAAAAATCCCCACGTTTTCGTCAATATTTTTATCAATTGAAAGTAATTCGCAAAATCTTATTCAAACTTTTGTATTTGGGTTTGAATATGTTCATTCTTCTGCTGAAACATTATCTTTTAAATATTTATCATAGTTAGCCAAGTCTGCAACACCAAAAAATCTTTTTAGCATTATTAATTTAACATGTAGTGGGCTATTTTCATGAATGCTAATGTTAGCTGCCAATGCAAGCTCTGGATGATTTATTAAAAAATCAATACCATGCTTCTTTAAATCATCTTCAATATTCTTATTTTTCTTTAACGTTTTTAGGTTATTATAAATATGATAAACTTCTGTTAATGTGCCAATTTCTAAGTGAAATGCATATTCAAGCGCCTCAATAATAGTTGCACTAACGTTATTAACTTCATTATTCATAATTGAATTGATATGTTTTATTGATAATCCAAGTCTTATAGATAATTCTTTTTGAGTCATTGAAAACATTTTTAAATAGTATTTTATTTCATCTGTAAAATCATTGCCATGAATTTTAACGTGCTTCATATCACAGATGTGCTCTTCAATCATAAACCCGCCTTAACTTAAACTATTTTTTTATTTTTTATAACTATATTATAATTTTTTTTAGTTAAAAAAGTTTATTTTTGATACAAAAAAATATACAAAAAAGTATTTTTATATTGTTTTTTGTATATTTTTTTAAAATGATAATTATTGTTAATATATTAAATTTAATATTCCTAATCCACTATATCTTTTGGTGCAACTGCAAAAGGTAGAATATCTTTCATTTTATAATGCTTTTGTTGCTTTAAATCAAAACTGTATAAATATACATCGCCATCTGGTTTTAAAAATTGGCTAATAACTTGGGCACAAGCACCGCAAGGATATAACAATTTAGATAAATTGCTAATTATATGTATTTCTTTAAAATTACCAACTCTAGCGCCATATGCTACAGAACCAAATAATGCACTTCTTTCAGCACATAATCCAGATGGAAATGCAGCGTTTTCAGTATTGACTCCCCAATATTCTTTACCATTTTCTTCAATTGCACAACATGCAACTTGAACATGTGAATAAGGACTAAATGAATATTTTAATTTATTTTTTAATTCTTCTATTGTCATATATATAATTTTACAATATATTTACAATATAAAATTTATATTAAAATAAACATTTTTTAAAAAGTGTTAAGTATTTTTGCTTAACAGTAAAAAATGTGCTAAAATATATACTAACTTTAAAAATAAAATTATTAATTAGAAAGGAAAAACCATGGTTAAATTAAGATTAAAAAGAACTGGTAAAAAATTCTATGCAACATATAAAATTGTTGCCGCAGATGCAAGAAGCCCACGTGATGGTAAATTTATTGAAGAATTAGGCTACTATGATCCTAATTCAAAAAAATTAGTGTTAAATAAGGAATTAATTATTAAACACTTGGACAACGGTGCAAAACCTTCAGACACTGTACGTAATTTGTTAAAGCAAGACAACTTCTATGCAGACTATGTTGCTTCTAAAAAATAATTTTAATAAATTATGAAGATAAACATTCTGACACTATTTCCAGATTATTTAAATGCTTTTTGCAATCAAAGTATGATAGCAAAAGCATTAGAGCTTGGACACATTGAAATAAACATCATTAATTTTAGAGATTATTCATTAGATAAACATCATAAAGTTGATGATAGCCCTTATGGTGGAGGCGCAGGTATGTTGTTGCAAATCGAACCAATAGATTTAGCACTACAACCTTTAAAAAATAGTCACAAAATTTTACTTTCACCGCAAGGAAAGGTATTTAGTCAAAAAAAAGCACATGAACTTGCAAAATTAAATGAAATTACTTTAATATGTGGGCATTATGAAGGCTTTGATGAAAGAATAACAAAATTAGTAGATGAAGAAATATCAATTGGAGATTTTATCTTAACAGGTGGAGAAATTGCAGCAATGGCAATTTCAGAAGCAGTAGCAAGATTAATACCAGGAGTATTAAGAAAAAATAGCTATGAAAATGAAAGCTTCGAAGGAAATGGATTACTAGATTATCCACAATATACAAGACCTGCCGTATATAAGGGAATGAAAGTACCAAAAATCTTATTAAGTGGAAATCACCTAGAAATAGAAAAATGAAGATTAAGAAAAGCTCGGGAAATAACCAGAAAAAAACGTCCTGAATTATTAAAGAAAGGGAAATAACATGAGAACAAAATTGTTAGAATTAGTAGAAAAAGATCAAATAAGAGAAGACTTGCCACAATTAAGAGAAGGTTATAACGTAAGAGTTCACGTAAGAATTAAAGAAGGCGATAAAGAAAGAATTCAAGTTTTTGAAGGATTAATTATTGCTGAATATGGTACAGGAATCAATAAATCAATTACCGTAAGAAAAGAATCATATGGTGTTGGAGTTGAAAGAATCTTTAAAATTAACTCACCTTTAATTGCTCAAATCGAAGTTATTAGACGCAATAAAGTAAGACGTGCTAAATTGTATTACATGAGAGATTTAAAAGGTAAGAGTGCTAGATTAAAAGAAATTAAAAACTCTAAATAATAAAAAAATAGGGCATAAGGCCTTATTTTTTTATTATTTAGGGCAATAAATTATATTACTTTAATTAACAAATTATTAACATTTTATTAACAAAAAAATTCAATATAATTGTTGGTAAAAAATTTTGCAATTTAATCAAATTAAATACAATTTTTAATATCTTTTTTTTCGACTTAAACTATAATCTTGTTCGAAAGGAAATATTAAAAAATGAAAAAACACACAAAAATTTTATTAGCATTAGGAAGCATTGCAACCTCATTTGCCGCAATACCATTAGTTGCTGCAGGTTGTGTAAAAACAAAAAAACCAGAAGTTAAACCAGAAGAACCTAAAAAACCAGAAGGTGAAAAACCAGGAACAACACCTGAAGATCCAAAGAAACCAGAAGGTGAAAAGCCAGGAACAACTCCAGAAGTTAAACCAGAGGATAAAAAATCAGAAGAACAAAAAGAAATAGATAATTGAATAGAAACTGTTAAAGACCAATTTGAATTTTCTCCATACAACAAAGAAGAAGTATTAAAATCATTAAAAGATAAAACAGGAATATTAGTATACAGTTACAAATCTCATAGTATAGTTTTAAAAGATAATAATGATGGTAAAGTAAATTGATCTAACATTGTCTTTTCTCCTAAAGATGAATCTTTATTCTCACAATATCAACTAGCCAATCCAACAAATCCTACTTATTATAATAAAAAATTCAAGAATAATTCAATAAGTGGTTTCATTGACTATGAATTAAAAGACAATAAGATAACATTAAAATTTAAAGGAGCTAAATTTGTTGCAAGAAAAGATCCTATTATTGGAACTAAAATTGTAAGCAATGAAATAGATCTATCAGAAACACAATTAGATAAAATTATAAAAGATCTTAAATTTGATATCAAAGACAAAAACAAAGAAATTAACAAATTAACTGCCGATGATGTTAAACTAGAAGGCAATCTAAACGGTGCAAATATTACTTATAATTTTGCAGCAAAAAAAGGTGATGAAACAACACTTGTTTTAACTGCTAAAATTTCAAAGAATTCCGTAACTCAAACTTTTGAAAGAGAAATTAAAGGATTTAAAAAACAAGAAACAAAACCCGAAACTAAAAAAGAAGACGAAGAATTAGATCCAAAAGTAAAATTGATCAACAAAAAAATTGAACAAAACAAAGACTTATTTACATTCAAGGTTTGAGATCAATCTAAAAAACAAGATGTAATTGAAAAACTAACAAAAAAAGAAGCTAAATTGTTTTATCTAAAAGGAAATCATAATATTATTATTGAATTTAATGGCCAAAAACCAAATGAAACCTTAAGTCAAGTTGTATTTGAAGGAAAATCCGGAGCATTTTGATCATTTGGTCAAGTTCAATTAATAAATCACCTACACCACTATAAAGATGAAAAGGTTGCAAAAGATAAATTAGAATTATCTTACTACTTAGAATATGAAGTAAAAGATAATAAAATTACTATAACTTGAGCTGGTGGGCAAAAAGATAGTGGAGTAGAACCTTTTATCTTTGGAACTAAAACCGCTTCACAAACAATTGAATTAAAATAACAAAACAAAAAAGTGGGCAACATTGCCTACTTTTTTATATGCTTTTTTTAATAGGAATTTATTAAATTCTTTTAAGTATGTAAAAGTCTTTTTTGCCTTTTTTTAATAGCGCATATTGACCATTAAATTTATTAAATGCAATTGAATAATTTTCATCGTTTATTATATTGTTATCAATTAAAAAAGTATTTTTTGAAATAAATTCTCTTGCTTCTCTATTTGAAGACACAATTTGATTATTTTTTAAAATTTCAATAAATTTTTCGCCTTCTTTAACTTCAATACATGACAAATAGCCTAAAAGCATCGAAATATCTTCTATGTTTAATTCTTCGTTACTTTTATTGAATAAAATCTCACTTATTCTAATACATTTGTTTGCAATATCAACTCCATGAATGTCTTTAACCAATTCAAAGGCTAATTTTTTTTGGGCAATTCTTTTGGTTTTATTTTCATTGTGTTGAGTCATAATTTCATTTATTTCTTCAACTTCTAAAAATGTTAATCATTTTAAATATTCTTCGATTTTTACATCACTTTGATTCAATAGGAATTGATATATAGAATATGGACTAGTCATATTTGGATCAAGCCATAACGAACCTCCGCCAGTGCTTTTACCAAATTTTTTGCCATTTTCGTCAACTAATAAATTGAATGTCATGGCAACAGCATCAGCAGATTCTCCATATAATTTTCTAATAATTTCTAGTCCTGTTGTAATATTTCCTCATTGGTCTGAACCACCCATTTGAATTTTTACATTATGATTTTCATATAGACATTTAAAATCTCAACCTTGAAGCAAGGTATATGAAAATTCTGTAAATGTTAAACCATTTGCAAGTCTAGTAGTAATAGAGTCTTTTTCAAGCAAATAATTTACATTAATTAATTTACCAATATTTCTTAAAAAATCTACTACTGTTCAATGCTTATAAAAATCTAAATTATCAATTACTTCTAAACCAAATTTTTGTAATTGACTTTTAATTGCATTTTTATTTTGTAGTAATGTTTCTTGATCTAGAAATTTTCTTTCAGAATTTCTAAAACTAGGATCACCAATCATTCCAGTTATTCCACCCAATATTGCAATTGGTTTATATCCTGCTTTTTTAAAACGTAACAAACTTAATATTTGTAGATAATTTCCAAGGTGTAAACTTTTAGCAGTTGGATCAAAACCTGTATAAACACCTGTTCCTAGTGATATATTATTAAATTTTTCTTCACTAGATATATTATTTAAAACACCTCTTGATTTCAAATCATCTAATATATTTTTTTGCATATCTATCCTTTTTGTTATAATGTATTAAATTATTTATTAAAAATATAAGTAATATAGTAATTATATATTAATTTTGTTTATTTTATGGCTATGAATCTTAAAATAAACAAATAGAAGGACATTATGAAAATTAAAATTATTATAGACTCATCAGCAGGAATGTCACAAGAACAAGCAAACAGCTTTGGATGAGATTTAATTCCAATTCAAATTGAAGTAGGAAATGATATATATAAAAGTGGTATAGATTTTGATACAGACAAATTTGAAGAACTTTGAAGAAATGACAAAAAAATAGATGCTAAAACTTTTGCAACTCCCCCTGGAATAGCCATGCAAGTTATAGAAAAATATTTTAATGACTACGATAAAATTTTAGTCTATCCAATTTCAAATGCGCTTTCTTCGCAATGTGCTTCACTAACAGCACAATTTGCTTCAAATCCAAAAGTTCATGTAGTACAATCAAATAAAATTTCTTATCTGATTGTTAGAGACTTATTAATGTTTGAAGAATATATTAAAGAAGGAAAAAGCTTCGATGAGGCAATAGAACACTTTAAAAATAATAATGAAAGATTACTTCTAATTCCGGAGTTTAACGATGCCTTGCTAAAAGGCGGACGTTTATCAAAAGCAGCAGCCGCAATAGCAAAATTATTAAAAATAGTTCCTATTATTAAATTTGATAACGGAATTTTAGAAAAAGATGGTATTGGTAGAATCTTTAAAAAAACTCTAGAAAAAAATTTAAAAGAATTATATGAAAATACAAACAAAGATAATGAAAACAATTATCTATTAGTTGTAAATGCAAATAACGATAAAATAGATGAATTAATTCAGTCGTTTAAACAAATAACCAATAATTTTCCAAGGATTTATACAATTCAATTGAGCTGCGACATTGTAGTTCATGCTGGTATTGGCGCAATCTGTTTAACATTTGCCGAAGTTAGTCCTAAAATAAAAGATAAATTCTTTTTATACTTAAAAAAACAATAAATTAAAAATACCAGGTTATTACTTGGTATTTTTAATTAGTTTATTTCTTGCTTTCAATTGGGTAAACTGATGCAAATTTTCTGTTAATTCTGCTTTCGAATTTTACAATACCATCGATTAAAGCAAATAAGGTGTCATCTTTTCCTCTACCAACATTTTGGCCTGGGAAAATTTTTGTTCCTCTTTGTCTATAAATAATGCTTCCTGCTAAAATAAATTGGCCGTCGGTTGCTTTTACGCCTAATCTTCTTCCCGCTGAATCACGGCTATTGGCGGTTGTTCCACCTGATTTCGTATGTGCCATAACTATTTTTCCTTAATTTCTGTAATTTTAACTCTGGTAAATGGTTGACGATGTCCTAATTTACGTTTATGAGTAGATTTAGCATTATGTCTATAAACAACTATTTTTTTTGCTTTTCCTTGTTTTTCGATGGTTCCTAAAACAGAAGCTTTTGGTAGATAAGGTGAGCCAACTTGTTCATCAATAACTAAAACTTTATCAAATGAAACTTTGTCACCTTCATTGCCATCAATTTTTTCGATGTAAATTACATCACCGCTTTTAACAAGTAATTGTTTTCCGCCGGTTTCAATAATAGCAAACATTATGAAGCCTCCTTATATTTTGTCACATCCTTAAGGTACTATTGATATAGTTTTAAAACCTTTTTAGTATGGTTACTCTATAAGTAACAAATACATTATAGCAAAAAAATATTTTTTTAATCAGTAAAAAGTGTTTCAAAAAATTTCTTTTATTTTTCAAACAATTTACTTGCTTTTTTAATTTCAATATCTTGTTTTTCTATTGAAACAAATATTTCATAAATTGTTGAACTTTTATCCTCTTCGGCGTTTTCTAGTAATAATATAGTAAATGCATCAACTGTAATATTTTTTAAACTTAATAAATTAATTTATTAAAAACCCCTATGCTAATTAATAGTAGTATTGCTTGATTAAAAAAAGAAAAACGATATATTCTGGCAAATAAAAATATTATTTCAAACCTTTTGGGTGATTTTAATTGCTTTTTTTGAAAGCATAGAAAAATGTGGGGTTAATAGAAATAAGAAAAAAAGCAAGATCATAACTGTCTTGCTTTTTTCTATAATTTAAAAAAAAGAAAGACTCGCATATTTTTATAGAGTATGAAAGTCTATTTTATATATCCTTTTTTTCATTTTTTAATTTGCTCACTACCTATTTTAAGGCCTTCTTTTATTCCGTTTAATTTCTCTTTAATTTCCATATTTCAAATTTCTTTGTCTTCGTTTTACAATTTTTAATATTCTTCAATAATATTTCTTGCTTCATTAACATAATTTTCTCTTAAATACGTTATTAAATCGTGAAATCTTCGATCTTTTGTGTTTTTATAATTTTCTTGTAAAATTAGTCTTAATTTTCTATTGCATCTATATATGCTTGGATTAAAGATTTCACAATTGCTTTTATTTGCATTTTAAATTCTTTAACTTTGCTTCTTAATTTCCCAAAAACAGATTTATTTATTTTTTTTGTATAATATAAGTGTAAATAATGAAATATAAAAGATAAGGAGATAATGTAATAGAAATAGTTGAATATTTACAAAAAAGACAATTTAGATAAATTTTTAAAAAGAGAAGATTTACCAAACTTTTGACCTATTTATGTTTATTGTGGGTCTGCCGGTTGAATTAAACCTTGAAAGTGAATTAGGGATGGATATCCTTGCTTAATATTTGGACTTACATACCTGATACTTGGGATAACCAAAAAAATAGGACATGCCGTTGTTGCATATGGTATGTATGATAATGGCAATAAACTTCCTTGCCATTATGGGTGAGCAGGCAGTTCTCAAGTTATTGTTTCTTCGAGTCTATCTGGCCAATTGTTTTTATTAGCTATAAAGCCAAATGGCAAAATTAAAAAACAAGACAACATTTTACTAAAAATGGAATTAAATATTCGGGAAAGGATTTTAAATAATGAAGAAAATTACATTAAAATGGTTAACAATTTTAGGAGCAACAACGCTTACTGCCTTGACTTTTTGTGCAATTTCTTGTAGTAATGAGGAAAAAGAAATTGAAAAAGCAGAGAACATTATTGGCGATAAAGAACTAACATTTAAAATAGATGATTTAAAATATGAAAATTCTGATAATTCTATATTGCAAAGTGCTAGAAGTATTGCAGGACCATGGCGCAAGGACTTTCTTAAGGTTGTTATAACGTCAAAATCAAATATTGAAGAATATTGAAAAGATAATTGAATTGTAGGAAAAATAGATTTAAAAAATAAGGCTATATTATTTTTAATACCTAGAGAAGTATTATTAGATGCTGATTTTATTAAAGAAAAAAATATGGATCTAAAATCTTCTTACCAAAAAGCAAACAAAGCATATTTAATAGAAAAACCAAAAAAAGTATATTCAAATCTAAAAAATAAACAAATATTAATAAATAACTACACTATATTTTTTGATGATATAAATAATGATAAATCTCCAAGAGTTTCAATGGCGATAAAAGAATTAAAATTTAATTTTGATCAAGACCGAAAAACCATAGAAATTAAACTTGGTTTAAGATATGAATTATCACAAAAACATAATAGGAAGTCTAATATATTTTTTCTTTCTCCTAAGGCAACCTTTAAATACATTTTAAGCGAAGAAATCAAATAAATTTATTTAATCAGCAAATATTGATTTAAAACATTAAAAAAGGGAATCATAGAAAAATCTGTAGTTAATTCAAATAAGAAAAAAGCAAGATCATAACTGTCTTGCTTTTTTCTATTATTCATAAAAAATAAGTTTAAAAAAATTAAAAAATCTTTAAAAGGAATTTATGGGTGCTAATAAAGAATTTATTTAATGCAGCAAGAGGCTAAATTAAATAACGATTATTAAAAGCTCTTTAATTCTTGTAAAAACCAAATTACGAATTTAAATAATTTAAGCATCGAAGATAAATTTCTAAAACAAAGAATTTTAAAAATTATAAATAATGAAATAAATGATTTTAAAAAAGTTGGTAATAAGATAATCAAAACCTTACTAAATGCCTATAAGCAAAACAATAATGCATTTACTATTTAAAAGAATTTCGCTTCATTTTTTAATGAGTTTTAAAAACAAAGTTAACTATTTTATTAAAAACAATAACAAATAAAAAACACCCTTATAAATATAGGGTGTTTGTGTTAATAAAATTAAATTCTTATTTAAATTTTAATTATTCAAAATCGTTTTGATTTTCTTCATCATCAAATGTTTCTTCTTCAGCAACAAGTTCTTCGTCGTTTGGAGAATAATCTTCTTCAACTGATTGTTTATCATTGTCATATTCTTGCGCTGTATTAACAACTGAATCAATATCAATAGGTTCAGAAATTTCTGTGCCGTCAATAGGAGTTGTTGTAAAGTAATTAATTGATGGTTTTATATCATATTTTGAATGTTCTTCAAATGGGAATAATGCTGTTCCTGATGGAATCTTTTTACCAAGAATAATATTTTCTTTTAAGCCAGATAAATTGTCAATTCTTGATGAAATAGCAGCGTGAACTAATATCTTGCTTGTTTCTTGATAAGAAGCAGCGGCAAGGAATGAATCAGATAACAATGGAACTTGTTTTGCACCTTTAATAACAACTTTACCATATGCTGGCTTCAAACCTTTTGAAAGTAGAATAGCATTTTCTTCTTGATAGTCAAAAATATCTACAATAGCACCAGCGAAGAATTTAGAATCTCCGGGTTCAGAAATTACTAATTTAGACATCATTTGACGAATTATAATTTCAATATACTTATCGCTAATTGCAATACCTTGAATACGATAAATTCTTTGAATTTCTTTCAATAAATAATTTTGAACAGTAATTACGTCTGTTAAAGCTAATAATTCTTTTAAGATAATTGGTCCTTCAGAAATCTTTTGACCAATTCTTAATTCATCACCTTCTTGAACACGTAATTTTTTGGTTGTATTAACAAAAATTTTGTGAGAAACTGTTGTGTTTGAAGATCCTTTGCTTTCAATTGTTACTAAATAGTTTGTATTGTCTTTTTCATCAACTTCAATCTTTGTAACAATACCTTTATATGGTGAAATAACGGCAGGACGTCCTCATGGTTGTTCGTGGGCATCAATTAATTCGATCAATCTAGTGAAACCACCAGTAATATCTTCAACCCCAGCAACACCACCAGTGTGGAATGTACGCATAGTTAATTGAGTTCCTGGTTCCCCAATAGATTGAGCTGCAATAATACCTACTGCTTCACCGATATTAACAATTCTATTTGTTGCAAGGTCTTTACCAAAACACATCTTACATACACCGTTACGTGTATTGCAGCCTAAGATTGATCTAATTTCGACTTCTTCAATACCACTTGCTATTATTCGTTGAGCAATTTTTTCAGTAATTAATTCATTTCTAGAACAAATTTCTATACCCGCACTTGTAACAACGGGCTTGTTTGCAAATCTTCCAACAATTCTTTCTTTCAAAGGAACAATAATTTGTTTAGTTTTTGTATCAACTATGTTTCTTGCTTTATAACCATAGTCAGTACCACAGTTTTCTTCTCTAACAACAATATTTTGAGCAACATCAACTAAACGTCTTGTTAAATAACCAGATTTTGCAGTGTTCAAGGCAGTATCGGTTAAACCTTTTCTAGCACCGTGAGTTGATGAATAGAATTCAAATGCAGTAAGTCCTTCAATAAATGAAGACTTAACAGGAACTTCAACTGTTGATCTAACAACACGGTCGTTTTTAGCATCGGCCTTAGTAGTTTTAGTATTGTTAGCCATCAATCCACGCATACCTGCTAATTGAACATAGTTAGAAATGTTACCACGAGCACCTGAGTTAATCATGGTAATAACTGGGTTTTGAGGATTTTCTTTAATTATTTTTTGTAGGTCTTCTTGAATTAAATCCTTAACTTCTGCTCATTTTTTAATAGTTAAAGAGTAACGATCATCATCAGTTATTCAACCTTGTTCATAGTAGTGTTTTAATTTAGCAATATATTCATCACCTTGAGCAATATATTCTTGCTTTTTATTAGTTTCTAGAATATCACTAAACGAAATAGAAATTCCAGAATATGTTGAATAGTCAAAACCTAATTGTTTAATACTATCCAATATTGAAGCAACAATGTTTGTATATCTAAATCATACAGTGTCTAGAATTTCTGCTTTTTCAGCTGGACTAAGTGGAACTTCTTGTCCATTTGCCCTAGCAGGATTTAACATTTCAATTTTTTGCTTTTCTTGACTTGTAAATTGAGCTAGCAACATTGCATGTTCATAATCAATTTTTCTATTTTCGTAATTTTTAACATGCAAGAATTGAATTATTGAATCATTAGCATCACTTGATTCAGTTAAATTATTAATAACTTCTGCAATATCAGACATTGAAATTTGAGCAACATATTTATCAAAAACATAACGTATTATTTTAGAAATATTCTTTTTGTTAAATGCTTCTTCAATAGGCATATTTTTGATATATTCAGGAATATTAGTACCTGCTTGAACGACATATTTTGCATTGAATGTTTGATTATAATCTTCCAATGAATTTGCCTTAGCAACTTTATTGTCGTAAATAAATGGAAATGTTTTAGGGAATATATTATTAAAAATAAATTTACCTACTGTTGAAATTATATATTGAGGACCATCAAATGGGAAAATAAGTTTTGGAGTTTTAACTTCTGAAGCAGGTAATGCTATTCTAGTGTGCAATGTAACTACTTTATTTTCTAATGCTCTAAGCATGTGTTCATAGTTATCAAAAACACGTCCTTCACCTTTAGCTCCTGGTTCTTCAATAGTTAGATAATAAATACCTAAAACCATATCTTGAGAAGGGTTAATAATAGGTTCACCATCTTTAGGTCCTAGAATGTTTTTATTAGCCAACATTAATTCACGACTTTCAAGCAATGCTTGTTCAGAAACAGGAACGTGAACAGCCATTTGGTCACCATCGAAGTCAGCATTAAATGGAGTACATACTAATGGGTGTAATCTAATAGCCTTGCCTCTTACTAAAACAGGTTCAAAGGCTTGAATAGATAAACGGTGTAATGTAGGTGCACGGTTTAATAATACTAGTCTGCCCTTAATCGCTTCAGCAACATGAGGTCAAATTACTGGGTTTTGGTCTTCAATAATTTTTTTAGCACTTTTAATTGTTGTAGCTACTTCTTTTTCAATTAGCCTTTCAATTATTCATGGTTCAAATAATTTAGCAGCCATTTCTCTTGGTATACCACATTGGTGCATTTTTAATGAAGGCCCTACGGCAATAACTGAACGTCCAGAATAGTCAACACGTTTTCCTAGTAAATTTTGTCTAAAACGACCTTTTTTACCAGTTAATGCATCTGAAATTGATTTAAATGGACGATTGTCTTTTGATAAAACAGGGTTAGGTGTTCTTCTTTGGTTATCAATTAAAGCATCTACTGCTTCTTGAATCATTCTTAGTTCGTTTTGAATAACCAATGTAGGGGCATCTTTTTCTTGTCATTGTTGCAATCTATTGTTTCTAATAATTACTCTACGATACAATTCATTAATATCGCTAGTTGAGTGTCTACCACCATCTAATTGAATTAAAGGTCTTAAATCTGCTGGAATAACAGGCAAATTATAAATTAACATGCTAGTTGGATCTTGTTTAGATTCAATAAATGCATTAATTACTTGCAAACGTTTATAAAGTTTTTCTCTTGTTTGTTTTGCAGTTGTGGCAAAACGTTCAGGATTATTAATTTCTTCTGCATTTAAACGATTAATTTCATCAGTTACTTTTTGCTTTTCTTCAGCTAAATTTATGTTTTTTAATAAATATTCAATAGCCAAAGCACCAGTACCTATTTTAGCGTCTGAATAATGTTCAATTACTTCGTTTAATTCATAAAAATCAATTCCATATTCTTGGCCGATTTTTGATGTTGCTTTTTCAATTAAAGAATCTAGAGTTTCTTTAATATCATCATATTCTTCTGTACCTTCTTGAAATCTTTCAAGTAATTCTTCTAGAGCATTGCGGTAAATGATTGCTGCTTCATTGATTTCAATAATTTGATTTTTAGGTAATGATTTTAAGCCACCATCTTCTAGAACAATATGATTTTTGTAATAAATTAAATTTTCTAGTTCTTCACGTTTAACAACGTCAGAACCATTTGAAGATTTTAAACCAAGCAATTTATAAATAATTGAGTGATCAACTTTGAAATATCAAAAGTGAACAACTGGACTATTTAGTTTTATGTGTCCCATATGGGTTCTACGTGCTATTTTAGGCAATATTTGAACATGTTCTTGTTTACATAATTCAGTTCTTGTACAATAGCTTCCTTCATTAACTTTTTTGTATTTGAAACCACATACAGGGCAACGATAATCAATCATTGGTCCAAAAATTATTTCATCAAATAATCCACCACGTTCTGGCTTATATGATTTATAGTTAATAGTTTCTGGCTTAGTAACTTCACCATGAGACCAAGTTTCAACATCTTGAGGAGTTGCTAATGCTAATGAAATTTTTGAAATTTTTTCTTCATCTAATGATTCGTATTTTGAAATTTTTTTCATATTATTCTAAACTCCCTTCAAATTCTTGGAAATATTTTGTGTCTACTCTTTGATCTTCTTCTGAATCATCTACAACATTATCAGATTTTTGATGTACTTCTAATTTAATTCCTAAACCACGTAATTCATATGCTAAAACATTAAACGATTCAGGTGTTCCTGGTTTAGGCAATCTTGCATCTCTAGCAAGAGAGTTATATAGTTTATTACGTCCAACAATATCGTCAGATTTATATGTTAATAATTCTTGTAGAACATTTGAAGCTCCATATGATTCAATAGCTCATGTTTCCATTTCACCAAATCTTTGACCACCATTTTGACTCTTTCCACCAAGTGGTTGTTGGGTAATCAATGAATAAGGTCCTACTGAACGTGCGTGCATTTTGTCATCAACCATGTGATACAACTTCAAATAGTACATAATCCCTACAGAAATTGGATGATCAAATGGTTCTCCAGTGGCCCCATCGTATACTATTGATTTTCCTGTTACATCAATATTTGCTTCTTCAAGAATATCACGAATTTGGTTGTGTGAAATTCCATCAAATATTGGTGTAACAAATTTTGTATTTAATTTTTTAGCAGCAAGTCCTAAGTGCAATTCTAGAACTTGACCAATATTCATACGTGAAGGGACACCTTGAGGGTTTAAAACAATATCTAATGGTGTTCCATCTGCTAAATAAGGCATTTCTTCAACAGGCAATACTATTGAAACAACACCTTTGTTTCCGTGACGACCAGCCATCTTGTCGCCGACTTGAATTTTACGTTTTTGAGCAATTGATACCTTAACTATTTTTTCAATGCCATCTTCTAATTGGTCGCCAAATTCTCTACTTAGAATATCAACAGCAACAACGGTTCCATTGTGGCCATGTTTTACTTTTAGAGAAGTATCTTTTCTTGAGGCAGTTTTTTGACCGAAAATTGCAGCCATTAATTTTTCTTCAGGAGTTGGGTTTTCTTCACCTTTTGGAGAAGTTCTTCCGACTAAAATATCACCTGCTGTTACTTCTGAACCAACTCTAACAATTCCATTTTCACCTAAGAAACGTTTAGAATAGTTTGAAACGTTAGGAATATCTGCAGTTAAAATATCTTCGCCAGCCTTTGAATGTCTAAATTGAATTGATTGTTCTTCAATGTGAATCGATGTATAAACATCATCCTTAACCAATCTTTCAGATAAAACAACGGCATCTTCATAGTTATAACCATGTCAAGTAGTAAAACCTACTAGAACATTTTTTCCTAATGCTAATTCTCCATCTTTCATTGAAGGACCATCAACTAATAAATCTCCTGCATTAATTTGTTGGCCAACTTTAACCAAAGGTACTTGGTGAATTAATGTACCTTGGTTACTTCTTTCAAATGTTCTTAAATGATAAACATCACTTTTTGAACTATTTTCAGGAGTTACTTTAATAACGTTAGCATCTACATAGTTAACAACACCTGATCTGGTTGCTCTAATATTGGTTGATGAATATTTAGCAATATCAGCTTCAACTCCTGTAGCAACTAATGGAGCTTCTGGGAATAACACTGGAACAGCTTGACGTTGCATGTTAGAACCCATCAATGCACGGTTAGCATCATCGTTTTCCAAAAATGGTATAGCCGAAGCAGCTATTGAGGTCATTTGACGGTTTGAGACACCAATAAAATCTACATCTTCAGCATTCACTTCTAAATATTCATTGTCACGTCTTACCATTACTTTTTCATCAACAATTACATTACCTTCAATGTGAACAGTTGATTGTGCAAATGTATATCCTGTTTCTTCAATTGCAGTAATGTAAACTGGTTCTGAAAAATCTACAACTTTTTTATTTACTCTGAAATATGGTGATTGAATAAAACCATATTTATCAATTTTAGCAAATGATGCTAAATTTAAAATTAAACCAATGTTTGGTCCTTCGGGTGTTTCAATAGGACAAATTCTTCCATAATGGGTAGGGTGAACGTCACGAACTTCAAATTGTGCAGTATCACGGTTCAACCCTCTTGGTCCTAGGGAGGTTATTCTTCTCTTGTTTGATATTTCAGCAAGAGGATTACATTGATCCATAAATTGAGAAAGTTTTGATGTGTTAAAGAACGATTTAAATTGATTAAATATTGGTTTATTGTTTGTAACGTTCTTAGCAGTAATCTTATCAATATCTTTTGCCGAGATTCTTTCTCTTGTATTTTTTTCTAATTTTAATAGCCCTATTCTAAATTGATTTTGTAATAATTCGCCTATTGTTACAATTCTTTTATTAACTAATGAGTCAGGATCATCATCAACCCCAACCTTTGAAAGTAAATTAAAATAATACGAAATCGTTGCAATTAAATCAGGAATCAACAAGAAATTTTCTGTTGATGTAGGATCATTACCTAAAACCAAAACTTTTTGGTCATGCAACATATCATTTTCAGTTGGATATATTCAAATGCTTGTTACAAATATTCTGCTTGCTAGTTGTTCATTTCCTTCAACTTCAAGTTGAGATCCATATGCATTTTCATCAATATCTGGCAATTTTCACATAGGAATAATTCCTTGTTTAAATTCATCATGAATCTTTCTTGCGATATTTCAAGTAATGAACATTCCTTTTTCATAAAGAAGTTCACCTTCACTAGAAAAAATATCTTCTGCTAAATAAGAATTAGCAATTCTTTCAATAATGTTTAATTTTCTATTTAAAGTGAAACGTCCTGTTTCAGTTAAACTATATCTTTTTTCATTAAACAAAGTTGTAGGAATTAAATTTCTAGCAGAATCAGCTGTCATACGATCGCCTTTTCTGATGATTCTATAAATTGCTTCTTGTGCTTCCCTTGTAACTTCTTCTGCATTAATTCCAGTGATTTTATCACGTTTAAATGTTTCTTCCAATTCTGGAATATTTTCGCCAAACATTCTTCTAATACCTGATTCACTAAATCCTAATGCTTTTAAGAAAGTTACAACTAAAAATGATTTGTTTTTATCGATATGTATTTTTACAGTATCTGGATTTTGAGAAGTTACTTTATGATAAATTTCTACTCATGAACCAATTTGAGGAATAATTTCTACTTTATTAAACAAGTCGTTGGCTTGTTTATTTCTAACATTAATTCCAAAATAAGCCCCGGTAGATCTAATTAATTGCGAAACAATAACTTTTTCTGAACCATTGATAATAAATGATCCACCATAAGTCATTATAGGAATTTCAGCAAATACTACTTCTGAATCTTCAACTTCACCTGTTTCGATATTGATTTGTCTTAGGTGCGCTTTTAACTTTCCTTGATATGATGAACCTTTTTGTTTAGCTTCTTTAATGCTTTCATATTCAGTTTTTTTAGGATATTCAAAATGTGCTGAATTATGAATGTATTCAATTCTAACTTTTCCATGTGCTTCAATTGGATAGATATTTCTTAATTCTTCTTCAATTCCAACTGTTAGAAATTTTTCAACTGATTCACGTTGCATTTCAAGAAAGTCAGGAGTTTCAAATTTCTTTTGACTAATTGAATAGTCACGTCTTAGGGTAATAGGTCCAAATTTTCTAATTTCGTATTTATCCTTATTCACCATGCTTTACCTCAATTTTTATTAAATTTTGTTTATATTTTTATTTTTTGTTGTATATACGCACACGTGCATAATATACGACAAAAAACACAAAAAAGGCTTTGTGTATTAAAAGCCTCACTTGTGTTTTTTATAATTAGTCTAAAGTAACTTCGGCTTTAGCTTCTGTTAATTTAGCTTTTAGTTCTTCAGCTTCTTCTTTTTTAGCATTTTCTTTAATTACAACAGGTGCTGATTCAACAAGTTTTTTAGCATCCATTAAACCTAGACCTAATAAGTCTTTAACAACTTTAATAACTGCTAGCTTGTTTCCACCATCTGATTTTAAAGTAACATTAAATACAGTTTTTTCTTCTGCTGCGTCAGCTGCTGCTGCTGGAGCTGCTGCTGCTACAACTGCTGTTGGATCAATTCCAAATTCTTCTTTTAATCCTTGAATTAATTCCATAACTTCTTTAATGTTCATTTCTTTTAATGCTGAAACAAATTCTTCTTTTGTTAATTTTGCCATGATAAATCTCCTTTAAAAATAATTATTCTGAAATTTTTCCTTGTTTTATTAATTCATTTAAACCAATTGATAATTGTTTCAATGGACTCAATAATGAATTTCCAAGAATCATAAGTGATTCTGTAAATGAAGGAAGTTTTGATATTTCGTCTAATGTTTTTTCATCAACTACTTTATTTTCATAAATTCCACTTACAATTCTTAATGCAGGGAATTTTTTCTTTAAGCCTACTAAGGTTTTTAATGCTGCTAATGAATCGCCTTTAACAAAGGCATAAGCATTTTGTAAAACTAAACTATCATTAATATTTGCATATTCTGTATTTTTTAATGCTTGTTTAACTAGTCTATTTTTGTAAACTTTTAGCAATGCATTATTTGGAGCTAATTCTTTTCTAATTTCTTGAAATGAAACAACATCCAAAGTAGCATAATTAATTACATATAATGCTTGTGATTCTTTTAAATTGTTTGAAATTTCTTCAACAACTAAAACCTTGGCATCTTTTAATGCGCTCATTATTATTCCTCCTATAAAGATTATGCAATGTACAAACAAGTATAAACATACTAGGTAACATTATTAAGCACTACGCCGTTACTGTCTTCATATATTGCGTTGAAATTATAACAAATTTTTAAAATTTTTATAATTTTTAAATAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATAACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCATCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTACGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCGTTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGCCATTTTGTAATTGGCACGTTTATAATTATACATATTTTTTTGTTTTTTTATAAAAAAATAAATTTATTTTATTGTTTGTTTTTTTATAAAGTTTTTAGCAATCAAAATTGCTTGATTTATTATATAACTTTAATTAATTTTTTTGTAAAAAATAGATATTTTTTTATTTGCTTATATATTTTTTTAAAAAATGCCTTAATAATTATTCATTAATAATTTAATTATTTATTTTTTTAAATTGAAGAGTTTGATAAATAAAAATTCTCTCGAATAAATCAAGAGAATTTTTTATTATTTAATATTATTTAATTTTATCGTTAATTGCTTCTTTAATAACTTTTCTTCCTCTATAAAAACCGCAGTTTTCACATGCTTGGTGAGGAGTAATTAAATTCTTACATTGTGAACATTCAACTAATGTTGGAGCCACTAGAGCGTGGTGTGATCTTCTTAAATGTTTTCTTTGTTTTGAAGTTTTTCTTTTTGGTACTACTGCCATAATTTTCTCCTTTAAATAATCAAATTATACCAATTCGATTATTGCCATTTTAGCATTATCACCAGCACGATTGGCTAATTTTGTAATTCTTGTATATCCACCATTACGTTCAACGTATTTTGGTGCCACAACATCAAATAAATATTGTAATGAATTTTTATTTTTGTTTTCTGTTGCAATTAATCTTAAATATTTGGCTGCTTGTCTACGCGAAGCTAGGGTATTTTTCTTACCTAGAGTAATCATTTTTTCTGCATTTGAACGAATTCTTTTAGCACGTTCTAATGTGGTTGTTACTTTGCCATTAATCAATAAATCTGTTACAAGTGATCTTTCAACGTGGTCTCATCACTCAGTATTTCTACGAAATAGTTGTTTTGGATTTGCCATTGTTATTCTCCTTCTTCGTTAGCAGCATATTCTTTGTTAGAATCTTCATTATTTAATTGACTATCAAGTCATTCTCTACGACGGTCAATAATATCTTGTACTGATTTTTTACCCAAATTTTTAATATTCGAAAGTTCTTCATCGCTTAATTTCATTATTTCATCAACATTATTAAATCCAGCACGTCTTAAGGCATTCAATGAACGAATTGTCAAATTCAATTTATCAATAGAAACTGAAACTTTTGGAGTTTTTTCATGTTTTTCTTTTTGATCATCGAATAAATCAATTGTTCCTAATGCGTCAATGTTTCCAATGATTTGGAAATGAGCAACTATAATCTTTGCTGCTTCTTCCATAACGTTTTTAGCTTCAACAGTTCCATCGGTTTCAACTTTAATTTTTAATCTTTCTTCAATTATGTTAGAAGTAGAATTTAATTCTTCAAATAAAATCGCAACTTTTTTAATAGGACTGAAGTCACTATCCATTGCCAAAAATTGACCATTTTTGATTTTTGATTCTAATTTTGGACCATATTCTAGAATGGTTTTTTTATTGTCTTCAAATGAAACATAGCCTCTACCGGTTCTTAAATATAATTCAAATTCCAATGAACCATTTTTTGATATTGTTGCTATGTATTGATCTTTATTAACAATGTCAAGTCCAGGGATAGTTTCAATATCCGAAGCATGAACTTCCCCTTCTTTTGTTGATTTAAATGAAATTTTAGCTAAATTATCTTTTTCAAATAATTCTGGGTCATATGCAAAGCGAATTTTTCTTATGTTTGCAACTAATGTAATAGCATCTTCTCTTAATCCTTCAATTGCAGTAAATTCATGTTCAACATGACTAATTTTTATTGCAAATGGAGCAACAGAAGTAATCGAAGAAAGTAATGTTCTTCTTATTACAGTTCCTATTGTGTTACCATAACCACGCATTAGTGGTTCAATAACGAATGTTGTATTAAAATCTGATACTTTTTCTGCTACTAATTCTTTATATGTAATTTTTTGTATTTTCTTCATATTTATTCCTTAAAAAACAAAATAACAAATTATCCACGTTTAAGAATCTTTTTAGGAGGACGAGTTCCATTGTGAGGAGTAGGTGTTACATCCTTCACTTCTTTAACACTTAAACCACTTGTTTCTATTGATTTTCTAGCAGTTGATTTTCCAGGACCTACACCTTTAACTAAAATTGTAACTTCCTTTAAACCAAATTCTTTTGCTTTTTCAACTGCAGCAGCAGCAGCAAGTCCTGCTGCATATGGGGTTTTCTTCTTTGTGCCTTTGTATCCTATTGCACCAGAAGAACTTCAAGCAAAAACATTTCCTTTTAGATCTGAGAATGAAACAATTGTGTTTTGGTAAGTTGAGTGAATATGTGCAATACCTTGAGTAATAACTTTTTTATTTTTCTTTGCCATTATTATTTACCTTTCTTTCCTGCTATTGTTTTTCTTGGACCTTTTCTTGTTCTTGCATTTTTCTTTGTAGATTGACCACGTACAGGTAATCCCTTACGGTGTCTAATACCACGATATGATTTAATTTCCATTAATCTTTTAATGTTTAGATTTATTTCTCTTCTTAAATCACCTTCAGTGGTGTATTTTTTAGCTTCATCTCTGATTCTAGTTAATTCTTCTTCGCTAAGATCTTTAACTCTTTTTTCGCCGTCAACATTAGCATCTTTTAAAATTTCTTTTGCTAGTGAAACGCCAATACCAAAGATGTAAGTAAGTGAAATACGAACTTTTTTATTATTAGGAATTTCAATGTTTAAAACTCTGGCCATGTTTTATCCTTGTCTTTGTTTATGTTTTGGGTTAATGCAAATTACGCGATTAACACCATGTCTTTTAATTATCTTGCAATTATTGCAAATACGCTTAATAGAAGCTCTGACTTTCATATTTCTCCTTATTATTTGTGTCTAAAAACAATTCTACCTCTGCTCATATCATATGGACTAAGTTCAACATCAACTAAATCCCCAGGGATCATTTTGATATTGTGAAGTGACATTTTACCTGAAATAGAAGCTTTAATAGTAAGCCCATTTTCTAATAAAACATCATAGTCTCTTGATGTGTGCATTTTAGTAATTTTGCCTGACATTTTGATTGCGTCTTTTGCCATTAAATATTGTCTCCTGAGAGTATATATGCTTGGTCATTGTCAATTAAAACTGTTTGTTCATAATGGGCAGTGTTATAACCGAGAGGATCATAAACTGTTCATCCATTTCCTTTGACCAATACATTTTTAGATTTTTGTAAAATCATTGGTTCAATGCAAATTACCATACCATTTCTAATTAGCTGTCCAGTTCCTGGATAGCCATCGTTATAAACATATGGATCTTCATGCAACTTTCTGCCTATTCCGTGACCTGTATATTCATCTGGAGTAAAATATCCTCTATCACGAATTAAATTTCCTATGGCTGCAGAAATATCACCAATGTGTTTGCCAACTTTAATTGCATTAAAGCCGGCATAAAAAGCGTCTTTTGCGCAGGCAATTAATTTTTTGTCTGCTTCAGTTATGTTGCCAACTCCTTTTGTAAATGCTGAATCAGAATAATAACCGTCATAAATTGCTCCAGTGTCAATTTTGACTATATCTCCATCCTTTACAACATAATCACTAGGAATTCCATGAATTAATTCATTATTAACAGAAATGCAACATGTATTGGGAAAACCATATTGTCCTAAGAATGCTGGTTTTCCACCCCTTTTTCTTATTTCTTTAAAAGCTACGCTATCAATTTCTTTTAAAGAAACGCCTGGTCTTATAAAGTCGTATAAAACAGTTTTCACTTCTGCCAGGATTTTACAAGCTTTTTTGATCTTTTCAATTTCAAATTGATTTTTTATTATGACCATTTTAAAGCCTCTTCTACATCATTATATACTTTATCAATTGTTTGATAACTATTTATCTTTAAAAGTATACCAAGTTCTTGATAAAAATTGATCAAACATTTAGTTTGTTCATTATATATTGATAAACGTTTCTTTATTACTTCTGGTTCGTCATCTGGTCTTTTTGTTACTTTTTCGTGGCATTTAATGCACAAATCACCAGGTAATGGTGGAAATGATTGCAAATGATAAATGGTTTTACAATTTAAACAAATTCTACGTTTTGAAAGACGGTCAATAATTTGTTCATCAGTAATTTCTAGCAAAATTACTTTATCAATTTTAATTCCAACATTTGCTAATGAAAGTAAAAACTTTGCTTGAGCTAATGTGCGGGGATATCCGTCTAATATGAAGTGTTTCTTTTCTCTTATTAATTCAGTTAATTTTTTTTCAACTAGTTGATTTGTTATAGAATCATCAACATATGCTCCCGAATCTAAAATAGATTTTATTTTTAAGCCAAGGGGAGTTTGATTTTTAATTTCTTGTCTAAACATGTCCCCAGTTGAAATTTGAACATAATCATTATTTTGCACCATTTTTGTTGCAATAGAGCCTTTACCAGCTCCTGGTGCGCCAAGCATAATTATATTAGGAAATTTTTTTGTGTGTTCGTTGTACACTTCTTGAGATTTTTCTATCATATTAAATCTTCTTCTTCCTCTCCATACATTTCTTTTAATTCTCTTATCATTTTTCTCTTTCTCGATAATTCTTGCGACTTGTAGCGGGCTTTAATTTGTTGAACAGTTTCATAAGCCGTTGAAACTAGTATCATTATACTTGTACCGCCAAATGCTATTGAGGCTGGCATGCCTAGCATTTGTTGAATAAATTGTAAAGCGCCTAGCAACAATAAATATCCTGAAGAAAAGAATGATAAACGTAAAATTACATTCAATAAATAATCTTCTGTTTGTTCTCCGGGTCTAACGCCAGGAATAAAAGTACTATTTTTAGCAAAATCTTCACTAATTTTATCAATTCTTGATTGTTGGATTCCCATTAATATTGTTAATCCAAATGTAATTAAAATAAATAAAAAGAATCCAAGTGGTTGAGTAAATTGCAAATTGTTGTAAACTCATTGATAATACTTGCTCGTATTTGGATCAAATAAGTTTGCAATCATTGTTGGCACACTCAATATCATCATTGAGAAGATAACTGACATGATACCAGCAGGATTTGCTTTAATAGGTAAATAACTCAATTCTTTTTCGTTTTTAGAAAGACCCGCACCAACTTGTTGAATTGGAATATGTCTTTCTGCAATTGTAAATATTACAACAATAAATATTACAAGTAAATAGCAAAAGATATATACTGCGTAATTTATTGCTTCTTGTAGAACTAAATTATTTTGTTGAGCTGATGGAACATAAAATTTAAACGCGCTCTTGAATGAAGGAATTAAAGCACTTGCTATACCAACAAAGATTATTAAACTCGTTCCATTGCCAACGCCTCTATTAGTTATTTGTTCTGATAAGAACAATGAAAAGAATGAGCCAGCTACTAATATTGCAGGCAAAATGAAATATTGATATGCTACCATTCCACTTTGTCCAAATAATTTTTCTAATTTTGGTGAAAAAGTAATTCCAAATCCTTGACGAGGATTAACTAATGCTTTTGTTATCAAATATGCTTGAACTATTGCAAAAACAAAAGTAACTCCATAAGTAATGAAATTTATTTTAATCCGGCCTTGAGGACCAGATTGACTTAAACGGTGAATGGCCGGGAATGCTTTAGTTTGCAATATCATCATAACTAAACTAGATGATATAAACGGTCCTATTCCTAGCGCGACAACAGAGAATTGTCTTAATCCACCGCCACCTACTAAATTCAAAATCCCAAAGAAATCTCCTTGGGTTAATGAATCAGGGTTTGCTAAATTGACACCTGGAATTGTAATTGTTCCAGTGGCTAGAAAAAGAGTAATAATGAATAAGGTGAAAAGAATCTTTTTAAAAAGATTATGATTTTTTCATCATTCATTTCAAGTGTTTTTTTTATCAACTAAAAACTTATCTAGTGCTAATTTGCGCTCGATCTTTTCTTGACTAATCTCTTTTTCCAACTTAGTTTTTCTAGCCATTATTTAACCTCTATTTTTCCTCCGACTTTTTCAATAGCTTCAATAGCGGATTGTGAAAGTGTTGGAATTTGTACATTAAGTTTTTTAGTTAAAGAACCCTTAGCTAAAATTTTAACTGGTAATGTTCCTTTAATCAAGTTTCTTGCAGCAAGTGTTTCGTAAGAAACTACTTCACCATCTTGATATTTTGATTGTAAATCTGCTAGATTTACTACTTGGAATTCAACGTGATTTACATTGCTAAATCCACGTTTTGGAATTCTTCTAAATAGTGGCAATTGGCCACCTTCAAATCCTAGTCTTACTTTGCTTCTTTTTGTTTGACCACTTTGTCCTCTACCGGCTTGTTTTCCTTTTCCAGCAGCATGACCACGACCAACACGGTGTTTGTCTTTTCTTGCCCCTGGAGTTGGTTTTAAATTATGTAATTGCATAATCACTCCTTATTTTGATAATAGATGATTTACATCAATATCGCGTAATTTTGCGATTTCTTCAACAGTTCTAACTTTTTTAAGTGCCTTAATAGTAGCTTGGACAATGTTTTGTTTTGTTCTTGAACCATAAGTTTTTGTTGAAATGTCGGTGTAACCTGCTAATTCAACAACAGCACGAACGGTGTTTGAAGCAATGATTCCGGCACCTTTTGGAGCTGGACGTAATTGGATTCTTGATGCTAAGAATTTATCTTGAATTTCATGAGGGATTGTTCCGTTTGAAATTGGAACTGAGAAAACATTTTTTTGTGCATCTTTAACTGCTTTTTTAATTGCATCTTGGACTTCATTAGCTTTTCCGTGTCCAAATCCAACTTTTCCTTTTTTGTCACCAACAACAACATAAGCTGAGAATGAAAATCTACGTCCACCCTTAACAACAGTTGTAACTCTTGCAATGTCAATAACTTTTTCTTCAAATTGTTTTGCTTGAGCTTGATTTGCTGAATGATCTTGATTACGATTTTGGAATTGTTTTGGTTTATTTTCGCTTGATTTTAGATCTTTTGAATTTGATCTTTCTCTTTTAAAAGAAACAACCTTTGTGCCTTCTGTTTTATTTTCTTTTAATTCAGGTTTTTCTGAATTTTTAATATTTTTTTCTACTTCAGCCATTAGAATTTAATCCCTTCTTCTCTTAGAGTATCAGCAAAAGCTGCTAATTTTCCGTGGTATATGTATCCTGAACGATCAAACACTATTGATTTTACATTGATTGCCAATAATTTTTTAGCAAAATCTTTTGCAACTAATTTAACTGCATCAATATTGTTTGCTTTATTTTCTAGTATTTGTGTTGATGAAGAAGCAATTGTGGTGTGCAATTCATCATTTACTGCTTGTGCATAAAATGCATGTAAAGATTTAAAAACAACAACTCTAGGAACTAATGCAGTTCCTTTTGACAATTTGTTAGTAATCTTTAAGTGTTTTGCTTTACGTTTTAAATTTCTTGATAACATAATTTATAACTCCTTAGGCTACTATTTAGCAGCTGTCTTTCCTTCCTTACGTCTAATAACTTCATCTTTATACATAATACCTTTTCCAGAATATGGACTTGGACGTCTTACATCACGAATATTTGCAGCAAATTCACCAACAGCTTGTTTGTCAATTCCTGAAATAATAATATTGGTTGGTTTTGGTAATTCAATTTTTAGATTTGATGGAACTTCTAAATTTACAGCATGTGAATAACCAGCAATAATTTCAATTGTATTTCCCTTTAAGGTAGCTTTGTAACCTACACCTTTAATTTCAATTTCTTTTTTGTAACCACTTGAAACACCTATTAACATATTTTTAATGTTTGCATTTGTTGTTCCGTGTAATTGTTTTGTAGATTTTTCTTCGTTAGATCTCAATGTCTTAATTTCGTTGTTTTCAATAACTACTGAAATTAATGGTGAGAATGTATAACTCAATTCTCCTAATTTGCCTTTTACTTCGATATGGTTTTTATCTAATTTAACTTCAACATCTTTTGGTATTTCTAAAACTCTTTTTCCAATTCTAGACATAATTACTTCTTTTTAAGTTTATTATCAAATGAAGGCGATAACTTCGCCACCAACATTAGCCTTACGAGCTTCTTTATCTGTTAATAAACCTTTTGATGTTGAAATAATAGCTGTTCCATATCCACTTAAAACTCTAGGCAATTTTTCAGCAGATGAATATACTTTTAAGCCAGGTTTTGAAACTTTTTTAAGACCAGAAATTGCTGATTGATTTTGATTCATACCTTTGTATTTTAAAGTAACTTTTATTTCTTTAAAATTGGTTTTTTCGTTTTTTACAACTTCAAAATCAACAATATATCCTTCTCTTTTAATAATTTCTAAAATTTTTGCTTTCTTGTTTGAATGTGGCAATACAACTTCATGATATTTACGAGAAATTGCATTTTTAATACGTACAAACATATCTGCAACTGGATCTATAATAATAGCCATAATTATCAGCTCGCTTTCTTAACACCAGGAAGTTTTCCTTCATGTGCTAATTTTCTAAAACAAATTCTACAAATTTTATATTTACGCAATACAGCGTGAACACGGCCACAAAGTTGGCAACGAGTATATTTTCTAACCTTAAATTTAGGTTGGCGTTCGGCTTTAACCTTTAATGATGTTCTTGCCATAATATCTCCTAATTATTGCCCTTTGCAAAAGGGATTCCTAAGTGTTTTAATAATGCAAATGCTTCTTTATTGCTTTTTGCAGTTGTTACAAGAATAACATCCATACCTTTTAATTTACGGATTTTATCAAATGAAATTTCAGGGAAAATAATTTCTTCTTTAATGCCGATAGCATAATTTCCACGGCCGTCAAAGCTTTTTAGTGATGTTCCATTGAAGTCACGTACACGAGGTAAAGCAACATTAATTAATTTTGTTAAAAATGATCACATCTTTTCTCTTCTTAGTGTAACTTTCCCTCCCATTGGCATTCCTTCACGTAATTTTCATGAAGCCAATGATTTCTTAGCGGTTGTTTGATATGGTTTTTGACCTGTAATTTGAGCAAGTTCAACCATAACTTCTTCGATTGCCTTTGAGTTTGAAACTTCGTTACCGGCAGTCATGTTAATAACGATTTTTTCTAGTTTAGGAGCTTGCATTACACTGGTGTAACCAAATTCTTTAACTAGCGCGAAACGAATTTCGTCTAAATATTTTTGTTCTAATGCTAATTTAATTTGTTTTGCCATATATTTGCTCCTTTCTAGATAGTCTTGTTAATTTTCTTCATCACTCTTTGTTTTTTGTTGGTTTTAGGATCAACTTTTCAACCAATTTTTGAACCGATTGATTTTTGACCTTGAGCACCTTTTTTAACCAAATAAGCAACGTTTGAAATATGAATTGGGTATTCTTTCTTTTCAATTTTTCCATCATTATTTTCTTGTGATGGTCTGTGGTGTTTAGTTTTAATGTTTACGCCTTTTAAAACAACAGTTTGTTCTTTTTGATTTGTTGATAAAACAGCAGCAAATTTTCCTTTGTCTTTACCAGCAATAATTAATACCATGTCATTTTTTCTAATTTTAGCTTGAGCCATATTATAAAACCTCCGGAGCTAATGAAACAATCTTTAAAAAACCTTTTTCACGTAATTCTCTTGCTACTGGACCAAATACCCGAGTTCCTCTTAATGAGTTATCTTCCTTAATTAATACAACAGCATTGTCATCAAATCTTATATGAGAACCATTTTCACGCATTAAACCACGTTTTGTTCTAACTATAACTGCTTTAACAACTTGGCCTTCTTTTACAATTCCGTTTGGAATAGCTTTTTTAATAGTTACAACAACAATATCACCAATGTTGGTTGATCTAACAATTGATCCACCTAGGTTTTTAATAATCATAACTTCTTTTGCGCCTGAATTGTCAGCAACGTTACATCTTGAAAATTCACTAAGCATTTTCTGCTCCTTCAATTGCGTGTGATTTGATTTCTACTAGTCTAAAGTGTTTGGTTTTTGATAAAGGTCTTGTTTCTTGAATTTTAACAATGTCACCAACTTTAGCTAAATTTTGTTCATCATGAACTGCGAATTTCTTTGATTTTTTAAAACGTTTTGAATAAAGAGGGTGTTTTTCGTAAATTTCTACAACAACAGTAATTGTTTTTTGGTTTTTAGTTGATACAACTGTTCCAACTAAAGTTTTACGACGATTTTCTCTAACTGTAGTTTCCATTATTTATTCCCTTCTAAAGTTTTTGCTTTAATTGCTGTTAAAGTTCTTGCGATATCTTTTTTAACTAATTGGATTTTGTGGGTTTCGTCTAATTGTCTTGTAGCATTTTTGAAACGTAATGAGAATAGTTCTGCTCTTAAATCAGCAACCAACTTATTTAGTTCGTCAAGTGATTTTTCTTTTAATTCTGCAAATGTCATAGTAATTATTCTCCTTCAACTTCATTAGTTTTAGTTTCTAAAGATTGTTCTTTTGAAATAACTTTTCAAGTTACAGGTAGTTTGTGACCACCTAATCTCAATGCTTCCTTCATGGTATCTACTGTATTGCCTAAAACTTCAAACATTACTGTTCCTTCTTTTACGACTGCAAATCATTGATCTGGGCTACCCTTACCAGATCCCATACGAACACCAATAGGTTTACTAGTTTTTGACATGTGAGGGAATATTTTAATAATAACTTTTCCTTCACGTCCCATATGTCTAGTAATAGCAATACGGGCTGCTTCGATTTGTCTTGCACTAATTCATGCTGATGAAGTTGATTTTAAACCAAATTCGCCAAATGCAACTGTATTATTACGGTGTGCAACAACTTTGTCGTGGCGGATACGGAACATTTTTCTATGTTTAGTTCTTTTTGGTTGAAGCATGTCTTTCACCGCCTTTTTTAAATTCTCTTTTACGTGGAGCTTCTTTTACTTCTGCTTCACTTGTTTTAGGTGATAAAATTTCGCCTAATGAAACTCATACTTTAACTCCTAAGATACCATATGTTGTTAAGGCTGTTGTTACAGCATAATCAACATCTTGTCTTAAAGTATGTAATTTCATTTCACCTTCGGTATATCCTTCTGTTCTAGCCATATCAACGCCATTTAGACGTCCAGCTACTGAAGTTTTAATACCTTTTGCGCCAGCTTTTAATGCTGCTTTAATTGCAAATTTTTGTGCTAGTCTAAAACTACCACGATTTTCTAATTTAATTGCAATTCCTTCTGCTAGCAATCTAGCATTTAAATCAGGATTTTTTAATTCAATTACTTCAATAGTAATGTCAGCTTGTTTTTTAGCAATAACCTTTTTTAATTGTGCTGTTAAGGCTTTGACATTTTCACCATTTGTTCCAAGGAATGCTGCAGGTTTTGCAGTGTGAATATAAACAATAGTATGTCCTGCTTGATCACGTTTAATTTGTACTTTACCAATTAAATATTCACGAACTTTTTTGTCAAAGAAATCATAAATTTTTTGATCTTCTAATAAATAAGTTGCAAATTTATCTTTATCTGCATATCAAGTTGTGTTATGAGATTTTGTAATTCCATAACGAAATCCATTTGGATTAACTTTTTGACCCATTATTTATCTCCTTTTTTGTTGATTTCTTCAACATTATTGATTTCTGTTAATTCAATAAAGAAATGACTTGTTCTTTTTAAAATTGCATAAGCACGTCCACGTGAGTGAGGTTGGAATCTTTTTAGTGTAGGTCCATCATTTACTAAAATGGTAGTTACATAAAGTTTAGTTGCATCAAGTCCAGCATTATTTGTTGCATTAGCAATTGCTGAATTTAGAAGTTTTAGAATAATTTCTGACGCTTTTTTGTGTGTATGTTTTAGAATAACTATTGCTTGAGTTGCTGATTTATATCTAATTAAATCTGCTACTAATCTTGCTTTACGTGGTGAAATTCTTTGCATGCGAACTGATGCATGTGCTGAATTTTGTAAAATTTCTTTAGCCATATTCTATTTCTTTCCTTTATCTGCACCATGACCATTGAAGGTTCTTGTTGGAGCAAATTCACCTAGTTTGTGTCCTACCATATCGTTTGTAACAAATACATCAATAAATGCATGTCCATTGTGAACTTGGAAGGTTAAACCGATAAATTCTGGATAAATTGTTGAGCGTCTTGATCATGTTTTAATTGGACGTTTTGGTGATTTATTTTCAATAATAGCCAAAACTTTCTTCATTAAATGATCATCAACAAAGGGAGCTTTCTTTAATGAACGAGCCATTATTTAGTTTCCTTTCTTCTTCTAATAATAAATTGATTTGATGCTTTTTTAGTTGCACGAGTTTTAACACCAAGTGCTCTTTTTCCTCATGGAGTCATAGGACTCTTTCTACCAATAGGTTGTCTACCTTCACCACCACCATGTGGGTGATCATTAGGGTTCATAGCTGAACCACGAACTGTTGGTCTAATTCCTCTTAAACGGTTACGTCCTGCCTTACCAATATTAACAAGTGAGTGTTCTTCGTTTCCTACTTCACCAACTGTTGCACGATTAATTGCTAAAACTTTTCTAACTTCGCCTGATTTTAATCTTAGAATTACATAACGACCTGATTCATCTTTACCTAAGATTTGTGCTGATGATCCAGCTGATCTAGCAATGATTCCACCTTGTCTTGGTTGTAATTCAATATTGTGAACAAATGTACCTTCAGGAATGTTGGCTAATGGTAAATGGTTACCAACTTGAATGTCAACATTATCACCTGACAAAATAACTTGTCCAATTTTAATACCTTTAGGGCTTAAAATATATCTTTTTTCACCATCTCTATAAACAACTAATGAAATATTAGCTGAACGATTAGGGTCATATTCAATAGTTTTTACAGTAGCTGGGATTCCATCTTTTTGACGTTTAAAATCAATGATACGATAGAATCTTTTCAATCTACCACCTTGATGACGGGTAGTGATTTTACCTTGATTGTTTCTTCCTGCTTTTGTTGGAAGAGCAACAAGCAATGATTTTTCAGGAGCATGTCCTGTTAAATTTGCTTGATAATCAAGAGAAGACATATTACGACGGCCATTGGTATATGCTTTAAATTTTTTAATAGCCATGTTGTTTTCTCCGTTCTTAAAAATATTGTTATCTTTTAAGAAAATATTAATTATTTTTTAATTTAGTTTCATCTTATATTTATGCAAGATTAACTATTTGTTTTCAGCAGCTTTTTTAGCTTCAGCTGCTTTAATTTTTTCTGCTGCTTTTAATTCTGCAGCGGTAGGTTCAGCAGGTTTTTCTTCTTTAACTTCTTTTTCTGCTGTTGCTTTTTCAGCTTCTTTGTTAGCTTTCTTTAATTCTTCAGCAAATAGAATAATTTTTGAATCTTTTGCTAAATATACAGTTGCTTTTTTAACTGAATTTTTAAAACCTGAGAAACGTCCTAATTTAGCAGGTTTTTTATCGTAATTAGTAATGTTAACTTTTAATACTTTTACATCAAAAATAAATTCAACTGCTTTTTTAACTTCGATTTTGTTTGTTCTTTTATCAACTACAAATGTATAAACGTTTTTATTTTCACGTGATAATTCACTTTTTTCTGTTAAAAGTGGGTATTTAATAACTTCATTTATATTCATTATTTAACCAATCCTTCTAAGTATTTAACATCATCTGAACTAATAACTAAAACATCTGCTGCCAATAAAGATTCAACTGATAAACTAGTTACTTTTGTTACAGTTACATTTGGTAAGTTTCTTGCTGATAAGAACACAGTTTCATTGTTTGAAACTAATAAAACCTTGTTTAGACTAGCAATTTTATCTTCGTTTAATTTAATTAATAATTCACGAGTTGAAATTTTTGATAAAGAATATTCTTTTACTAAAACAGCATGGTCTTTTGCTAATAAAGTTAATGCTGATAATAAAGCGTTTCTTCTTGTTTTTTTGTTGATTTTTAGATTGTAGTTTCTTTCAGTTGTTGGGCTGAATACTTTACCACCACCAACAAATATAGGTGATCTTAGTGATCCAGCACGAGCATTACCAGTATGTTTTTGATCTCATGGTTTCTTACCACTTCCACTAACTTCTCCACGAGTTTTAGTTTTGTGAGTAGCAAGTCTTCTTGAGCCACGTTCTGATAAAATTGCATCAAAAATTGCTTGGTTATAAATTTTTTCTAAACCAAATACTGATTTAGGAAGATTTTCACTTGAGAATTCAACGTGTTCTTCATTATCTTTTTTAACTGATATTTTTGATGGTTTTTTAGGAAGAGTTTTTTCTACTAGATTTTCTTTTTCTAAGTAGTCGATTGCTTCTTCATTTTTAACTTTTTCGCCAGTTACTTTTTCTAAAATTAATTTAGCTTTTTCTAATCCAACTGATCCGCGGTAAGCGCCATCACGGTGGAATCATACTCTAGTATTGTTTTTTGATTTTTCAGAAAGTTCAATAAATTCTGTTAATGCTTCTGAAAAATTTTCAAAATTCTTGAATGATTTTTGGTTAGCCTTTTTTAAATTAAATGAAATATTACGATTTTCATCAAATTTTTCAGAAATGTAGAATTTTTCTAATGTTACATTTTTTGTTGCCATTATTTATTTTCTCCTTCTTCTCCGGCCTTAGCAATTGCTTCATCCAAAGCAGCTTTCATTTCTTTTAATGACATATCCATGTTTAATTCAAGATTGTATTTTTTAGCTTGAGTAAAGATTTCATTTTTAGCCAAAGCTTCTTTTAGATTCAATAATTTAATAACTGGTTTATTCTTAATTGATTTCTTAGCAGTTTTAATGATTAAGAATGCTCCTTTAGCACCAGGAACTGATCCCTTGATCAATAAAATATTGTTTTCTGCATCTTCTTTAACAACTTCTAGGTTTTGAACAGTTACTTGTTCAGCTCCTAAATGTCCTGGCATGGTCATACCTTTTCAAACCCTATTTCCAGAAATATCTCCTAGAGAACCGGTTTGTCTTACAGGTTGTGAACCACCACCACCACCGTGTGATTTAGGGCCAATATGTTGATTTCATCTCTTGATGGTACCCGCAAATCCTTTACCTTTAGAAATAGCTGTTACATCAACAACTTCTCCAGCGGTAAATAATGAAGCATCTACAGTATCACCTAATGAAAATCCTGACATATCACGGATTTCTTTTACGAAGCGCTTAGGTGTTGTATTTGCTTGTTTAAAGTAATTAACTTCAGGTTTAATGATTCTTGAAGATTTTTTATCTTCTGTTGCTAATTGAAGAGCAACATAACCATTTTTTTCTGATGTTAAAACCTTAGTAACAACATTTGGTTTAACTTCTACTACTGTCACAGGTATTAATTTACCTTCTGAAGTAAATAGTTGAGTCATCCCAACTTTTCTTCCTAAGATTCCTTTCATGATTATCCTTTCAAATTTATTAATTTATTAAATATATATACTATATTTATAAATAAAAAATAATTACTGTTATTGATTAACAGTTTTTAAACTTATTTGTACACCAGCAGATAAATCTAATCTCTTAGCCTTATCAACTAATGATTCAGAAACTTTATCTAAAACGATTAAACGTTTATGAGTTCTGCTTTCAAATTGTTCACGAGATTTCTTGTTAACGTGAACAGATCTTAAGATGGTAATAATTTCTTTATGTGTTGGTAAAGGAATTGGACCACTAATAGTTGCTTTTTCAGCTTGAGCTAATAAAACTATTTTTTTAGCAGCTTCATCTACTAAGTGGGCATCAAAAGATTTAATTTTGACTTCTAGTTTCATATTTGTTCTCCTTCTTCGTAATTCGAAGTAACTCCACACAAGTTCTCAATATGCGAGATGAACAACTAAGCTCGGCATATCGTAACCTTGTGTTTCTTCGTTGTAGTTGCAAAAGAATTATAACAAAATTTTTATTTTTTAAAAAAATAATTTTTTTTGATGAAAAAAACATGCTTTTTACTTTTGATTTTATTAAAAAATTAACGACAAAAAATAATTGACTTATACTAAAAAATATAGATATTAAAAAGCAATATAAATAATTAATTTCTTATAAAAAATATTAAAAATAGTTGTGTAAGGTCAGTAAGCATATCATAAGATATATAAAAAGCTACGTTGGCTATGTTTTATATCACTAAAAGAATTTGTTATCTGTAAGTATAATAGTCCTTTTTTGTTTTTAAGTTTTTTATAAATAAATCAAACTATGCTATAATGCATTCATATGAACCTAGAGAGATACACGTAAGTAATCGAACAGTAGCGTGCTAGTTCGTGTAGCTACAATTACTCTAGGCTGGTTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATAATTTTTATATATGCTAGAAGCATGAAAACCCTTAAAAATAACAAATCCTTTATTAAAAGGTTTTTTTATCACCAATTGATGGCAAAGTTTTAAATAATGTAAAAATTAAAGCTCGCCCACTGATAACTTTAAGAATGTTACAAAATTAAATTTATTTGTAGTATAATTTTTGTAACTTAAGAAAGGATATTTATAGGTAATCGAATAGTAGGCAGCCTATAAACTTTAATTTTTTGTAAGGCCTAATGATATGTCTTAAGTCTCGGTCGTCTTAAAAAGAGGTTTATACCTCTTTTTTTGTATAATTTTTAAACGTTTTTACATCTTATTTAATTTATTTATGATAAAATTTAATCGAATTTAGGGCAATACACGTATGCAATCGAACAGTAGCGTGCTAGTTCGTGTAGCTACATTCTCCCTAAACTGGTTCGTCTTAAAAAGAGGTTTATACCTCTTTTTTTGTATAATTTTTATATATGCTAGAAGCATGAAAACCATTAAAAATTACAAATCCTTTATTAAAATGACACCAGATTTAAAGTTCATCCCTTGATAATTTTTAAACATTTTTACATCTTATTTAATCTATTTATAATATAATTAGTTTAATTAGCTTGAGGCAATACTTATAAGTAATCGAAAAATAGGCAAGCTTATAAGGCTTATGTGTTAAATGTCTATTGATACCCCTCTAAGCTCTAGTTCGTCTTAAAAAGAGGTTTGTTCCTCTTTTTTTGTATAGTTTTTATATGAGTTCAGAATTATGAAAACCATTAAAAATTACTAACCCTTTACTAGAAAATATTTTTTGTCTAAATATCGACACCAAATTTAAAGTTCACTTTTTGATAATTTTTAAACAACATTTTTACGTCTTATTTAATCTATTTATGATATAATTTAATTGAATTTAGGGCGATACACGTATGTAATCGAACAGTAGCGTGCTAGTTCGTGTAGCTACATTCCCCCTAAACTGGTTCGTCTTTAAAAGAGGTTTGTTCCTCTTTTTTTGTATAGTTTTTATATGAATTTAAAACTATGAAAACCATTAAAAATTACTAACTCTTTATTAGAAGATATTTTTAAGTTGCCAATTGATGGCAAAGTTTTAAATAATAAAAAAATTCAAGCTCACCCCTTATAATCTTTGAAAATATTATAAAATTAAATCGTTTTACGATATAATGAATTCATATGAACCTAGAGAGATGCATATAAGTAATCGAACAGTAGCAAGCTTATATGTAGCTACAATGCTCTAGGCTGGGTCGTCTTAAAAAGAGGTTTGTTCCTCTTTTTTTGTATAGTTTTTATGTGAATTTAAAACCATGAAAACCTTTAAAAATTGCTAGCTCTTTATTAGAAGATATTTTTTGTCTAAATATCGACACAAAATTCAAAATTCATCCTTTGATAATCTTTGAAAATATTATAAAATTAAATTTAATTTATTTGTACTATAATTATTGTAACTTAAGAAAAGGGTTTATGGGTAATCGAATAGTAGGCAGCCTATAAACTTTAATTTTTTGTAAGGCCTAATGATATGTCTTAAGTCTTGATTCGTCTTAAAAAGAGGTTTATTCCTCTTTTTTTGTATCCTTTACTAGAAAATATTTTTAAATCAACAATCGATGGCAAAGTTTTAAATATTGTAAAAATTTAAGCTCACCCCTAATAACCTTTGAGAATGTTACAAAATTAAATCGTTTTACGCTATGATGTATTCATATGAACCTAGATAGATGCATATAAGTAATCGAACAGTAGCAAGCTTATATGTAGCTACATTACTCTAGGCTGGGGTCGTCTTAAAAAGAGGTTATTCCTCTTGTAAAAATGAAAATTCAAGTGCCCCCAACTAAATTTTATTTCAAATTTGTGTTGCACTTGAATTTTCATTTTGACAACTAGCAATTTGATTTTCAAATTGTTAGTTTTCTATTTATTTTTTTAATTTTTAAAATAATCCTATTAATCAGCATCCGAAAGATCTAAGTAAATATATCCTTCCGATTCTTTACCCATTAAATTGTTTTTAATCATATCTGGACAAAGAATTTGTATATAAATACAAGGCATATTCTTTTCTTCACCTTTTTTTAAAAATTCAATTAAAAGTTTGTGACCTCAAGGCATAAGATTTTCAGGAATTTTTATATCAAAATATTTTTTAATGTCATTAGCTTCCATAAAATATGTATAAGCAATTCTTTCTTGACTTTTTAACATTTCAATAGTGTCGTATTTTTTAGTTTCATCAATTGCTTTATTAACTATTTCAATTAAGGCTTTAGCTCCATTATTGGCTACAAATTTTTTACCCTCTTTAGAAAGTTCAAATGAAAATTGTGTTGTTATTTCTGAAATTTGTGAAGCGGATAATAGCTTTCTTTGAAACATTCTATGAAGTCTATCGGGTTGTACGGGATCTGTATCATCAGTATTTTCGTTATTTGTATCTTTATTGTCATCTTTCTTGTCGTTAGTATCGGCGGTACCGGTATTTGTGCTATCTGTATTTTTGTCATTAGTATCTGTAGTGCCAACACTAGAATTACCTATATTCTTATTATCAGTATCAGTAGTACCAGTATTTGTGCTATTTGTATTTTTATCATTAGTATCTGTAGTGCCACTATTATCTATATCAATAGTGTTATTACTTGTATCTAAGGTATTATTAATTGTGGTAGTTTCCTTGCAAGATATGCTTAAAAGAGGTAATAATGACACAGCTGGAAAAATAAAACTTAATAAAATATTTTTTTTCATATTCCCTTCACCTTTTAATAACTATGATTATACAACAATACGGCAAAATATATTAGTCAAAAAATTTTTAAATTTGAGCAAAATAATGTTTATATTTCTTTACATTTAGTAAAATTTAATTACTAGATAACAATATTTTGTTTTTTAGTTTAAATTTCAAATAATAAAATAATAATAAAAATTAAATCAAGGAGAAATATGATTTCTAAAATTTATGATGATAAAAAATATCTTGAAAAAATGGATAAATGATTTAGAGCTGCTAATTATTTAGGCGTATGTCAAATGTATTTAAGAGACAACCCTTTATTAAAAAAGCCTTTAACATCAAATGATATTAAACTATATCCTATTGGACACTGAGGAACAGTTCCTGGACAAAACTTTATTTATACACATTTAAATAGAGTAATTAAGAAATATGACTTAAATATGTTTTATATTGAAGGTCCCGGACACGGTGGACAAGTTATGATTTCAAACTCTTATCTTGATGGATCTTATTCAGAAATATATCCTGAAATATCTCAAGATGAAGCTGGACTTGCAAAAATGTTCAAAAGATTCTCATTCCCTGGTGGAACTGCAAGCCATGCAGCACCTGAAACACCTGGTTCAATTCACGAAGGTGGTGAATTAGGATATTCTATAAGCCATGGAACAGGAGCAATATTAGATAACCCTGATGTTATTTGTGCAGCTGTTGTTGGCGATGGCGAAGCAGAAACAGGCCCACTAGCAACTAGCTGATTCTCAAATGCCTTTATTAACCCTGTAAATGATGGGGTAATACTTCCTATATTACATTTGAATGGTGGAAAAATTTCAAACCCTACACTTCTTTCAAGAAAACCAAAAGAAGAAATTAAAAAATACTTCGAAGGTTTAGGCTGAAATCCAATTTTCGTTGAATGAAGCGAAGATAAAAGTAATCTTGACATGCATGAATTAATGGCAAAATCATTAGATAAAGCTATTGAATCAATTAAAGAAATTCAAGCAGAAGCAAGAAAAAAACCTGCTGAAGAAGCAACAAGACCAACTTGACCTATGATTGTTTTAAGAACTCCTAAGGGATGAACTGGACCAAAGCAATGAAACAACGAAGCTATTGAAGGAAGCTTTAGAGCTCACCAAGTTCCTATTCCTGTTTCAGCATTCAAGATGGAAAAAATTGCTGACTTAGAAAAATGATTAAAATCATATAAACCAGAAGAATTATTTGATGAAAATGGAACTATAATAAAAGAAATCAGAGATCTTGCACCAGAAGGTCTAAAGAGAATGGCTGTTAATCCAATTACAAATGGCGGAATCGATAGCAAACCTTTAAAATTACAAGATTGAAAAAAATATGCTTTAAAAATTGACTATCCCGGAGAAATAAAAGCCCAAGATATGGCAGAAATGGCAAAATTTGCTGCTGATATAATGAAAGATAACCCTTCATCATTTAGAGTATTTGGACCTGATGAAACTAAATCAAATCGTATGTTTGCATTATTCAATGTAACAAATAGACAATGACTAGAACCAGTTTCTAAGAAATACGATGAATGAATTTCACCTGCAGGCAGAATTATTGACTCGCAATTAAGTGAACACCAATGTGAAGGTTTCCTAGAAGGTTACGTATTAACCGGAAGACATGGTTTCTTTGCATCATATGAAGCATTTTTAAGAGTTGTTGATTCAATGCTTACTCAACATATGAAATGAATTAAGAAAGCTTCAGAATTATCATGAAGAAAGACCTACCCTTCATTAAATATTATTGCAACTTCAAACGCTTTCCAACAAGACCACAATGGTTACACTCACCAAGACCCAGGACTTTTAGGACATTTAGCTGATAAAAGACCAGAAATTATTAGAGAATACTTACCAGCTGATACAAACAGTTTACTAGCAGTAATGAATAAAGCCTTAACTGAAAGAAATGTAATTAACTTAATTGTTGCTTCAAAACAACCAAGAGAACAATTCTTTACAGTTGAAGATGCTGAAGAATTACTAGAAAAAGGTTATAAAGTTGTTCCTTGAGCTTCAAATATTTCAGAAAATGAAGAACCAGATATCGTATTTGCTTCAAGTGGTGTTGAACCAAACATTGAATCATTGGCTGCTATTTCACTAATTAACCAAGAATATCCACATCTAAAAATAAGATATGTTTATGTTCTTGACTTATTAAAATTGAGATCTAGAAAAATAGACCCAAGAGGAATTAGCGATGAAGAATTTGATAAGGTATTTACTAAAAATAAACCTATCATATTTGCTTTCCATGGATTTGAAGGATTATTAAGAGACATCTTCTTTACAAGATCTAACCACAATTTAATTGCACACGGATATCGTGAAAACGGCGATATTACAACATCGTTCGATATTAGACAATTAAGTGAAATGGATAGATATCATATTGCTAAAGATGCAGCAGAAGCAGTATATGGAAAAGATGCAAAAGCATTTATGAATAAACTAGACCAAAAACTAGAATATCATAGAAATTACATTGATGAATATGGATATGATATGCCAGAAGTTGTTGAATGAAAATGAAAGAACATAAACAAAGAAAATTAATTTTAGTTTAAATAATTTCTTATATTTATGGTAAAATTGACTAAGTTAATTAACGAAAAATAACATAGGAGTACACATGCAAAAAGATATTCACCCAAAATACAACCAAATTAAAGCTGTTTGCTCATCATGCAATTCAACAATAGAATTTGGTTCAACTGCTGATAAAGTTACTTTAGATGTTTGCTCTAATTGTCATGCTTTCTACACAGGAAACCGTACAGATGTTAAAGCTCGTGGACGTGTTGAAAGATTTAATAAGATATTAGAAAAATCACATAAAAAGAACTAATTAATTAGCATTAATAAATAGGCAAAATGCCTATTTTTTTATTATGTGCAATAATGCATACCAACTGCATAACCAATGTTTTCATTGCTATTGTCTCATTTCAATGAAATATTTCCAGCTTTTACTTCTCCTTTATTTGCATTTAAAGTCTTAGCTAAGCCTATTGATGTTCCAATAATTCCAACAGTGCTTGTTACTACAGACAAAATAGTTGAAATTAAAGCAGTAATTGCAAAACCTCCTACAATGTTTTTTGTTTCTTCTTCGCTCATTAGTTTGAAATTTATATTCATTTTTTATTTCTCCCTTCAATTTCAAATATAACAAATATTCCATTATGTTGAATGTATTGAAGAAAAAATAGCGAAAAGTAGCAATCTTTTTCGCTATTAGTAATTTAAAATTGTTATGCCCTCGCTATTTGCTGATATTGCTAATAAATCTAAAAATATTGCATATTTAATCTCGTTGGGTCTTAAACAAATGTTATATTCATTCTTTATCACTATTTTCTTTAGTAATACTTATTTCTTATTTGATAAACAAAAAATTTAAAGTCAATACTATTTATAAGTTGATATTTAGAATTATTAGTTCTTCAATAATTATTATTGTATTGACATTGATAAATAACTTTACAAACTATTTTGTAACCTTTGTATTATATTCATTACTAATATTAATTGGATTATTTGCGAATAAAAAATTAATTTAAAAATTAAGCAAGTTAAAGAACAAATAAATAGAGTTTAAAATTCTATGAATAAAAAATTAGACTCATATAACTTAAGGGTTGCGAGTCTAATTTTTATTTTGTATTTTTATTATTATTTTGTTGAATCAAAAGTATTTTCACTATCAACGTTTTCATTATTGGTTAGTTGTTTTTCTTTACTCTTATTTTGTTTATTATGAAAGCACAAACCTATTATTATTGCAGAAATTATTGTTCCAATTCCTCCACAAATTAAAAATGAATCTGCGAAATTAAATGTTCCTTTATCAATTCATGGAAAATATATAATATCTCTTACACCTTGAAATTGAAATCTATCATACATATTTCCAAGCGAACCTGCTGCTGTAACTGCTAGTCCTGCAACTACAAAAATATATCAATGATTTTTAAAAAATAAACTACCAATAAAGGTTGCAAAAATTATAATAAAACTTATTATGTGCAATCCAACGTTGCCTAATCCTAATTCCAAAGTTGTTCCAGCATGGAATACAGTTCTATATCATAAAAATGCATGTGCTCCGGGTTGACCAAATGAATAAATAGCTTCGGAATCTCTATAATCTGCCGGAATATAATAATTTCTTTTAGTTAATAAATCAATTAAAGCAAATATTATAAATATTCCTATGTAAACAGAAGCATTTATTAAAGCATTTTTTCATTGGCTCTTCCAATATTCTTTAGTAAAAATGGAAATTTTTTTATTTTGCATCAATTACATCCTTGCATCTTAAACAAATTTGCAATTCATCATCATAATCTGAATCTTCAAAATGGTTTCAACATCTCAAACATTTTTTGGTTTCTAATTCTCTTACTTCAATGGTATTGCCAAACGTTACCTTTGCCACCATTAATAATCTATTTAATGGCAATGATTTTATAAAATCTGAATCGCTTTTTATTACTACTCATGCTTCATTTGGTCTCTTTATTGCTTGCGATTTAATTTTTTCTTCAATTAATTTGTATACTTCATCTTTTATTTCAAAGAATTCTTTTCATTGATCTTGTAATTTAAAATCAAAATCTTTTCTTTCTAAAAATGGCAATAAATGAACGGATGGCAACTTGTTAGGTTTATTGTAATATTTATAAATTTCTTCGGTTGTAGTTGGCAATATTGGGGCAAGAGCTTTTAATAAAACCTCAATAATGTTACTAAAAACATATTGAACCATTCTACGTTCAGGGTCATCTTGTTTTTCAAGATATAAAATATCTTTGGTTATAGAGATATAGTAACTACTTAAATCAATAACAAAATTATTAATATCTTTAATAACATTAACAAATCTATATTGTTCGTAATTCAATAATATATTTGCTTCTAGATTATGCAATTTTTCTAACATTAAAGCATGAATTGAAGCTGGCGTAATTTCTTTAAAATCAAAGTCACTAATTCCACCAAGCATGAACTTAATTGTATTTCTAACTTTACGATAAATTTCAGTATTTTGGTTTAATATCTTGTCGTCAATTGTAACATCAGCAGTATATTCAGAATTTGCTGCTCAAAGTCTTAAAATATCTGCTCCATATTTATCAATCACAGCCAAAGGATCAACAACATTGCCTTTTGATTTTGACATTTTTTGACCTTTGCCATCTAAAACAAACCCATGAGAAACCAAGGCTTTAAATGGGCTAGTATTTCTTCAAGCTACTGAGTTAATTAATGAACTATTGAATCAACCACGATATTGATCTGAACCTTCTAAATAAAGATCAAATGGGGCTTTTGTTCCGCCTGGTTCAACGCTTATTGAAGTTGAGCCTGAGTCAAATCAAACATCCATAATGTCTGTTTCTTTTTTAAGACCTAAATTTCTAAATTTTTCTGGCAATAATTCATCAGCTGTTCGTTCGTATCAAACATCGCTGCCATGTTCTTTAACTAGATTAATTACATGTTCAAAAATTTCATCATCTAGAACAGGATTTTTGTCTTTATCATAAAAAATAATTATTGGAACTCCCCAAGATCTTTGACGAGAAATACATCAACTTTCACGATTTTCCAACATTAAACTTAAACGTTTTTTGCTTCATTCATTGAATGTTTTGACTTCACTCAACGCCTTAATGATTTGTTGTTTTATTGGTTTTAATGAAACAAATCATTGTGGTGTTGCTCTATACATAATTGGTAAATGAGTTCTTCAATCGTGAGGATATGAGTGTTTAATTTTCTTAAACGCAATTAATTGATTATTTTCTGCTAAAAATTTGCCAATTAAAGGATTTGCATCATCGTAAAAAACACCATTATATTCAAAACCTTTATCATTAATTGTTCCATCATCGTTTATGTGCATAATCATTTCTAGATTTTCTTTTTTGCCGATAATAAAGTCATCTTCACCAAATAATGGAGCAATGTGAACTAAGCCAGTACCAGTATCTAAACTAACATGATGACCAAAAACAACTGGCCCCACCAATGGGGTTATAGGATTTTTATATTTAATTCCTAAAAATTCTTTACCTTTGAATGTTGATAGAACTTGATATTCTTCTCATTTAGCAACTGCAGCAACTGATTCAACTAAATCTTTTGCAATTATATATTTATTGCCATTTACTAACACTTTTGCATATTCAAATTGATCATTAATTGCAACTCCAGAGTTAGCCAACAATGTTCATGGGGTTGTTGTTCATATAATTAAATAATCTCCATTTTCAACACATTTGTTTCCTTCGACTACTTTGAATGCAACATATAGAGAAGGTGAAATGTGGTCAGCATATTCGACTTCTGCCTCAGCAAGTGCAGATTGAGAAGAAGGTGATCAATAAACTGGTTTCAAATCGCGATAAATTAGGCCTTCTTTAACCATTTTAGCAAAAAGTTCTAATTGCTTAAATTCTAAGTTTTTATCCAATGTCACATAAATTTCTGAAAAGTCTGTCAATAAACTTAATTTTTTAAATTGAGCCTTTTGATGTTCAACTTGACTTAAAGCATATTCTGCTGCCTTCTTTCTTAATTCAATAACACTAAAATCTTTAGCATTCTTTTTAGCTTCTAGCAACATTTTATGTTCAATAGGCAAACCATGTGTATCTCAACCCGGAACATATGGAGAATAATATCCCTGCATACTTCTAAATCTTACAATTATATCTTTTAAAATTTTGTTTAGAGCGTGTCCAACATGAATATTGCCATTTGCATATGGCGGACCGTCATGTAATACAAATGATTGGCTGTTCTTATTTCTATTTAAAATAGTTTGATACAACTTAATATCTAGCCATTGCTTTTGATATTCCAATTCTTTTTCAACTAAATTTGCTTTCATTGGAAAATCAGTATTTGGCATCAATAACGTGTCTTTATAATCTTTCTTTTCTCCCATTATTAACAAACCTTTCTTTTTCTATTTAATTATGAAATTTAAAATTTTAAAATCTTTATATATTACTTTAACTATTTCATGACCTTGTAATCATTTAGCAACATTGTCTAGTTTCTTTGCATTTTCAATAATTTCTTCATTGCTTCAATTTGGCAATATTTCAATTTGAGAACGAACTTTTCCGTTAATTTGAATACCAATTTGTATATTTTTAATAATTATCTTGGTTTCATCAGCATAAGGTCAAGTTTGATATTGAATTGGTTTTTCATTCAACATAAACAAAAGTTCTTCAGCTAAAAATGGCGCAAACGGACTTAATAAAATAGCAAATTGTTTTAAAACAAATGATGATGAAATAGTTTTTAAAGACGTTAAGTAATTAGTAAACACCATCATTTTGCTTATTGCAATATTAAACTTAGTTTTTTCAATATTTTCGCTAACTTCTAAAATCAATTTATTAATGTTGCTTATTAATTCATTATTACTATCGTTTATATCAATATTTCCTTTGACAAAATATTCAAATATATTGTAGATTTTTTCAATTCATCTTCTTATACCATTTAATGAATCTGTACTTCAAGATTTTGATTCCACCAAAGGACCCATAAACATTTCATAAACTCTTAAACTATCTGCTCCATGAGATAAAATAAAATCATCAGGATTTATAACATTACCTAGAGATTTAGACATTTTTTGACCATCAGGTCCCATAATTAATCCTTGGTTTATTAGCTTTGTAAATGGTTCTTTTGTATTAACAATACCCAAATCAAATAAAAATCTATGTCAAAATCTTGCATACAATAAATGCAAAACAGCGTGTTCTTGCCCGCCAATATACAAATCAACAGGCATTCATTCTTCAAAACGCTTTTTTGCTTCCAATGAATTTAGTGGAGTATACGAACCATCTGGATTTTTTAATATATAAGCCAAATAATATCAACTTGAGCCAGCCCATTGAGGCATAACATTTGTGTCATGATGATATTTTTTGCCATTAATTTCAACATTCATTCAACTTTCAACATTTGCCAGTGGACCCGAGGTTGTGCCTGATGGTTTTATGTTTTTTATCTTTGGCAATTCAACTAATTCTTTTACTAGATAAATATTATTTTCTTCATCAAATAAAACAGGAAATGGTTCTCCCCAATATCTTTGCCTTGAAAAAATTCAATCGCGTAATTTATAACTTACTTTTTCATCTGCCAAATTATTTGCTTTCAATTCATTAAAAATACATTTTACTGCCCCTGCATTATCCAAACCATTTGCAATATCTGAATTGATATGAATACCATCTCCTAAAAATGGTAATTCATCACATTCAATAATTTTTTTAATCTTTAAATCAAATTTTTTAGCAAATTCATAATCTCTTTGATCATGACCAGGCACGCACATTACTGCCCCGCTTCCATATGACATCAAAACATAATCAGAAACTCAAATAGGAATTAATTCATTAGTCAATGGATGAATTGCATACGAACCAGTAAAATATCCTGTTTTATCTTTATTTAAAGATGTTCTTTCTAATTCATTTTTCAATTTAACTTCTTTTAAATAGTTAGAAATATCATCATGATATTTTGCGGTAGTTATTTTTTGAAGAAGTGGGTGTTCGGGAGAAATTACTATTGCAGTTACACCATAGATAGTATCCAATCTAGTAGTAAATGTTGAGAGTTGCAATTTTTGTTGTTCGCTCGAACTAATATTTCACTTAACGATATGTCCTTTACTTCTTCCAATTCAATTTCTTTGCAATTGTTTTAAACTTTCAGGTCAATCTAAATCTTCTAGGCCTTCAAGTAATTTATCTGCATAGGCTGTTATTTTTAACACTCATTGTTTCATAGGCTTTTTAACGACAGGAAAATTACCCCGTTCGCTAACTTTAATACCGTTTTTTTCTAAAATTTCTTCATTGGCTAAAACAGTACCTAGCCCTTCGCATCAATTAACATCAATTTCTTCAATTGAAGCTAGTCCTTGTTTATACATTTCTTTGAATATTCATTGAGTTCAAATATAAAATTTAGGATCAGTAGTATTTACTTCCTTGTCATAATCATAAGAAAATCCCATTGCTTGCAATTGTCTTCTAAAATTAGCAATATTTTTTAATGTAAAAGGTTCAGGATCATTCCCTGTTTTTATTGCGTATTGTTCAGCAGGTAGGCCAAAAGCATCTCAGCCAATAGGATGCAACACATCAAAACCATTTAGGCGTTTATACCTAGCAATAATATCAGTAGCGGTATAACCCTCTAAATGCCCAACGTGCAATCCCATTCCCGAAGGGTAAGGAAACATGTCAAGTATATAGGCTTTTTTGTTGCTATTATTTCCTGTGGCAAACGATTTATTTTCGGCTCAAAATTTTCTTCATTTTTGTTCTATTTCATTGTGCTTATACATAATATTTAAATTTTACATTAATATTAATTAAAAAAATAAGAAATAAAAAAAGTTGCGAAAATTTAATCGATCCAAAAAAAATAATTAATTTAAAAATAAGCAAATGTATTGAAATTTCTAATTAATATGTTTAAAATTTTTCAATATTTAGAAAATATTACAAATTTTTATATCTATCCTGTAAAATTTACTTATTTAAAGACTAAAAACAAAATAAAGTTAAAAAAGTTTTAGTTTTATTAATAATAGAAATAAATTTAATCAAAGAGGTATAAACATGAAAAGAAAAATTTTACTATTTACCCCCTTAATTATTGCTGGTAGCTTAATACCAACAACATTGACAGCATGCAATAATGAAGATAGCAAACTTACAAAGCAATTAAAAGCAAAGCAAACATTTAATGATTTAAAGAATAAATTGCAAGATCTATTGAACTCAGAAGATGCAAAAGATATTGATGCAAATAAAGAAATTAATACACTTGCAAACACAATAATTGATGATAATTCTCAAATACAAGACATTGAAAATAAAATCAAAACTATTGAAGATGCTATTGAATCATTGACTAAGAAAATTAATGATAAAAAAATCTTAATAAAACTAAGACAAGATTTTAACAAAGCAAAAGAAGATCTAAAAAATCTTTTGGAATCAAAAGAAGTAGCGGATATTGAAAAAGAAACCGAATTAAATACTCTAAATACAACTGATATTTCCAAAGATTCTTTACAACAAGAAATTCAAACCAAAATACAAAAAATTCAAGAAGCTATACAATCAATTAACAAAAAAATCAATGACAAAAAGCAATTAAACAAAGAACAACAAAATCTTGTTGAAGCAAAAAAGACACTTGAAAATTTAATTGCTACAGAAGATGCTAAAACAGTTGGAATTGATGAAGCAAAAAAAGTTCTTGACAATAATAAAAATAATAAAATATCAACTATTGAAGAAATTAAAAAAGCAATAAACGATTTGAACGATATTACAGCCAAATTAAATCAAAAAATTGCAAACAAAAAGCAAGAATTATTAGAAACTTTCAAAAAATCTAAACAAGATTTAGAAGAATATATAAATAAAAATTTAAATGAAATCAGATTTATTGAAGTTAAAGAAAAAGCCCAAAAAACCATTGAAAAATTTTCTAATATAAACAAAGATAATTCCTCAATCAAAGAAATTGAAGATGCTACTAGCTCGCTAAATAAAGCTAAAGAAGTTGCTTTGAAAGAAGAAGAAGAAATCAGAAAATTAAACGAAGTAATAAATTTTATTGCCAAAAGAAATGAACTAGATAAATTAATAAAATCATCTAATATTAAATCTAGTGAAGATGTTATGAAAAAGGCTAAAAAAACTTTAGACGATAATATGTCTGTTGATACTAATAAATTATCTTCGAAAGATTTTGAAGAAAAAAGAAAAAATATTGAAGAAGCAATAAATGAACTAAAGGCAGCATTAGAAGCAACAAAAAAATCTAAAAATTAAAGATCAATATGTATTGATGTATTCATTCAATGTAGTTAAACCCAAAATAATTTAAGTTGTTATAAATTATTTTGGGTTTTAATATTAAATGATTTTTGCATATTATATTTTTATTCAAAAAATATATATACTGACATTAAAAAACAATTAAATGTTAAGATTATAAATATAAATTAATTTATTTTAAAATTATGGAGTGTAAAAATCTATGGGTAAAAAAGCAAAATTATTTGTCTTTGGCGGGATTAGTTTTTTAGCGGGAGCTTCAATTATTGGTTTTGTTGCAGCATATGCTTCAATTAATAAAAAGAATGAAATTAGAGTTAGCGAATATAATAAATTTCTTGAAGAAAAAACTAATTTAAATTCTTTTGACAATTGAGAAAATGATAAAAAAATTAGTTCAATAAAATCTAATACCTTAAGTGAAGTTGAAAAGATAGATAAAAATTCAAATTTATTTACTATTCAAAAAGCCCGCAAAATAGTCAACGAATCCATTGAAAATATCAAAAATATAATAGCATCGCATAAAAAAATGTTATTAGAAAAAGAAAAAGCGTTTATTCAATATAGCAATGTAAAAAAGCAATTGCAAGATATGCTTTTAAAAATTTTTGACTTTTTGAATAAAGAAAGCCAAGAACATTATTACATTGGCGATGGAAACGAAAAAATAATAAACAAACAAAATTGACTTTTAAAAGAAATAAATGAAAATCAAAAATATTTTTCAAACATAACGATTGATTCGGATATAGATAGCATAGAATTGGCAACAACTAAACTGGTTGAAATGAAATTTTTAATTAATGATAAACAAGAAGCATATAAATTTGCCAAAGAATATAACGACTTATTAAAAGAAATCAAGCAATTTATTTCAGATTGATATGAATTATTAAAAGATCCTGATATAAAGAAACATGAAGGTCCTTATGGTTACAACACGCCAGAAACTTTGTTCGTATATTTTTTATTTAAATACAAAAATGATGACAAAAAAATATTTACAAAATTTAAAAATTATGCACAAGAAATGTATGACAAAACTGCACTTCTTGATGAATTCGACATTGATTTTTTAGGATTTAAAATCAAGGATAAGAACTTCTATAAAAATTATATTGATTTGTTTGCACCATATAAAAAGGCTATTGATTTGTGAAAAAATTATTATGATTCATATTCTATAGGGCTAAAAGAACTAAAAGAAATTATTTGAGACAAATTGTATATTAATGGTTATACTATCTTTTTTAAACCTAAAAAAATATATGCTTTGATAAATCAACCCGAACTAGACAAAGAACTAACTGAAACTACTGATGATTATAAATTTAAACTAATGCTTATTCCAAAAGAAAATACTATAGAACATAATAAATTAATAGAAAATTTTAAAAACTATTGCGACGATTTAATGAATAGAAGCAAAAAATTAAAAAATGACAATCAATAAAGGAGACACGAAAATGAAAAAAAGAGCAAAAATTCTAACCTCATTAAGTCTTTGCTTAACTACTGCTATTTCAACAATAGCAATCTATTCAATAATCGCCGTCAAAAAAAATATAGAACTAGATAAGTCAAATAAATATTCATTATTTATACAAGAAAAATCTAGATTGCAATCGCTTGTTGCAAATAATCAACACAAAATTCCAAATAGCTATTTAAATTCAATTCAATATTTTCATAATATAACTAAAACCTCAAAAGCAATAGAAATAATTGATGCAACATCAAAAATAAGAAGGGAAATTATTGACGTAAATAAAAAAATTCTTGCCTTTGATTTAGAAATAAAAAATCAATTATCGCTATTCAATGAATTTAATCAAATAAAACAAAAGCTTGAAAATGATTACCATAACAGACTATATAAAATATGAAGAGCAGATACTGGAGAACCAGATATGATAGTTGATTTTGGTTTTCTTGAAGAAGAAACTCATTTTCAAAAATATCTTGAAACAATTAAAAAATATTCTTGAATTTCTAAAAATTCATCATCATTTGAAATAAAAAGGGCTATCTTTGATTTAAAAAATATTGAAAATAATATTGATTATTTTTTAATTAAAAAAGAAGTTGAACATTTATTTGAAAAAATTGAAAGCACTATTTCAATAGTTGAAACTAGACTAGAAAAAAATAAAATCAATAAAATATTTGAGGATAATGAAGTAAAAGAAATTTTAGATTTAATTTCACAGTTTAAATTAGATTTACAAAAACAAAAAGATTTATATAACTCAGTAGACAGTAAAAGTGATAACATCAAACACAAAACTTTTAAAGAAATATTTAGCCATTATAATAAAAACAATTTCTTTAAAATTTTTGATTGCATAGATCAAAGAGTTGAATTATATTACGACTTTATGTTCTTTTACTCACCTTTATATTTTTATGAACCAACAGAAGAAATCAAAAAAGTTTTCCAATATCCTGATAAAAAAGATTTTGAAAAAGAAATAATTTTAAAATATAATCAAGCCAAACAAGATCTTACTAACTTCAAATCAAAGATAAATGGAAATGAAAAAATTGCCAAAAAAGCCTACAATAAATTCAATGAATATATACGCAATTTAGCAATAAGTTTTGAAAAGGAATTAATACAAAAAAAATTATTATTATTAAAAAATTTATTAACCAAATATGATCAAAAGCAAGATGGAAAATCACAATTCATAACAGTCGAAATAAAAAAAACCATAAATGATGCAGAATCTTTATTAAGTTATAATGACTTAAAATATTCCACAAGAGATAAAAAGAGTATTTGAGAATTTAGAACAGTAAAAAATAATTTGCAAGAATTAATAGACAAATATTAAAAATCCTTGAATTGTCCTGCCTTTGCAATAATCAAAAGATTATTTCATACACAATACTAAAAACAAGATAATTGAAAATCATTATCTTGTTTTTATTTTTAATTTGGCAATATTGAAATTAAATTTGATTGACTTTTTGTCTAATTAAATACAGTTTATTTTGTAATTTAAGTAATATCTCGATTAAATATTTTAAATTTGGCTGTGATTTCTTAATATAAAAATCTTCTAATTTTTCTTCGTGTTCTTCTATAAGTTTTCTAACTTCTTGTATTTCGTTAGAAATTTTTTGATTTTTAGAATAGACATCATTTGCAAGAATATTAAATTCCTCAACATTTGATGATATCAATTTCAATAAATTTCACCTGTTTAAAATAATTTCTAAGTTTAATTTTCTAGAAAGTTCGTATGTAATTTCTATATTTTTATTGATTTTGGCAATATCTTTTGAAGAAAATAACTCAGAATTTTCTTTTAATATTTTGGCTATTTCATTATTTAGAAATTTACTTAAAAAATTTCCATCTTCTGAATTTTTTTGTTCTTTTAAACTTTCATTAAGCATTTTTAAAAGTTTAATGCTTTCTTCTTTATTAGTAATGTTTAAAAAATCCTTATTTATATTTTTATAAATTTCTTCTTGGACAGTTAGAATTTTTTTAAATTCTTCATGTATAGATTTTTTTATTTGTTGATCATTTCTATTGTTTTCCAATTTATTAATTGTTGCTAAAAAATCTGTAATTTTATTTTTTGTTTCTTGACTAAATAAATTAAATAAATCGCTATTTTTTTGTTGCATTTCAAATTCATTCTTTGCATTATAAATAATTTGTGAATATAATTCAATATATTCGGCCAATAATTTATTCGATTCTTCAAATTGATAAATAACTTCTGCAAATGTTTTTGAATCAATATCCTTAAAATCATTAAATAATTCATTCAACATTAGCCTATCCACAACATCTCATGTTTTACTGTTTTTAAAATTATTAAAGTTAATCAATTCTTGTATAAGAGGATTTAAATCATTTTTAACATTATTTAAATGTTTTTGTATATCTTTTGATGAATTTATTTTTTGATAAATATCTTCAATTCTTTTTGAAATTTCACTAGTTAATTTCGCTATATCATCATTTTTAAAACCAAAATTAACATCATTAATCTTTTTAAGAAGTTCTATTTCTTTGCTCATATCTGCTTTGTTTCATTTTTTATAAATGCTAAATAGATTTTCTAATTTTCTTTTTTGTTTTAAAAATTCAACATCAATTTTTGATTTTGGACTTAAGGACTCTTTAGTATATCTTTGGTCAATTAAATTCTTTTGAACGCTAATGTTTCAAAGAATTTCTTTAGATTTCAAAATAAATTCATCTGCCTCATTCTTTTCTTTTTCAATGAAATCTTTGATTTCTAATTTTAAATTTTCATAAATAAAATCTTTAAGATTATTGGCTATATATGTTTGAAGTTCATTTTTAACTTCTTGTAACCTACTAGGGCTAGAACTTGTCGCATTATTTTTATCTTCTTTTGGTTTTTGAGATTCGTTTGGAACAGTTGGCTTTATATTAGGACTAACTATGTGGTTATTATCTTCTTGTTTTTCTTTAGATATATTTGGTTTGACATTAGGCGTATTTGCTACATTGTTGTTTGAATCTTCTTTTTTATCTTTAGTTTCTTCTTTTTTATCTTGTTTATTAGAACATGAAGCACTAGAAATTACTGTAAATACAGTTGCAAACGATCCTAAGATAGTTGATAGAATAAATAGTTTGTTTTTTCGTTTCATTTGTGTTTCCTTTGATTTGATATTAATAAATTATTTTTAAATAATTATTAATATAATATAGAAATATCAAAAAACTTATATTTAATTTAATTAATTTTTAAAAAATTAATAAATTATTCTATTAAAAGTACAAAATTAGATTTGCTAACAAAAAATATATTTTTTTAACTTTTGTTTCTAATATATTATATAATTATTAAGCAATTTAAAGGAGGGGTAGCGAAGCGGCCAAACGCGGGTGGCTGTAACCCACTTCCTCACGGTTCGGGGGTTCGAATCCCTCCCCCTCCACCATGTTGCCCTTTAGCCAAGTGGAAAGGCAGCGGTTTTTGGTACCGCCATCCGTTAGTTCGAATCTAACAAGGGCAGCCATTTCATTAAATTGTCCAAAAAAGCGCAATGCGCTTTTTTATTTTATTAAAAACCTTCCGATTTTTAAGTTTTACTATCGGAAGGTTTTTAATAAATTATTTATATTATTTGTTCATATTATATTTATTTTTATATTCAAGTAATTTGGCTTGTATTTCAGCTTCTTTATTTTTTAATTCTAAATTGCTATGTTCTCCTGGGTTGTCCAAAATTTTTTCTGCTTCTTGTATTAATTTGTTTAAAGCTTCGTAGCCTTCTTTATATTCAACTTTTCCTTTAAGATTTTTTTCAATAAAAGAAGATGTATTACTATTTGTGCCATACAAATTATTTATGAAATCAAAAAATTCACCAATAATTTCATTCATCTTATCTTCAATTGGTTTTTTTAATGCTCTAAAATCAGATTTAAATTTTTCTAATTTATCTTCCTGTATTCCTTGTTCTTGTAACTCAAGAGCATCCATTCTAATCCTTTCAATTTCTTTTTTTGGTTCTTCTCAATATTTTTTGCTATCCAAACAAGTTAAAGATTTGCTTTGCATATCTCCTATAAATTCAGGTACTTCTAGATTTACGAAGCAAGATATATCGCAACTAAATTTATCTTCCTTTGCATTGTTTAGGTCTTTGGTTAATCATTGCTTAGCCACGGATATTTGTTGAATGTCAGAAGCAGAAGTAATATCTTTAAAGAGATTAAGTTGTGCGTTTAAATGGTCTATTATTTTTTTATAATCATAATAGTTACTATTATATTCTTTATAATATTCATCCTCTTTTACTTTTTCAATAAATTGTTTAATTTGTTCCCTAATTTTTTCAAGCCCTTCATATTCAATATTTTCCTTTGGTTTTTTATCATTAACTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTTCTTGTTTATCAACATCTTTAGCTTCATTTGATTTAATTAAATTATCTAATTGTTGTTTCTTTAAATTAAAGTCTTGTGTGGCTTTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCATTCACAATTTGATCGGTCGTCGATGAAATATCTACATTGTTATTTTTCAATGATTGATTTGCATTAGATTTGTCGACTTTTTGTGCGTCTTGTGAATTGATTAAGTCTTCTAATTGCTTTTTAGCGTCATTAAAGTCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTAGTCAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGACTCAATGTCTTTAATTAAATCATTGTTAGTAAGATTTGTGTTATTAAAGATGTTGGTTTCTGATTGTTTATCAACATCTTTGGCTTCATTTGATTTAATCAAATTATCTAGTTGTTGTTTCTTTGAATCAAAGTTTTGTTTAGCATTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCATTAACAATTTGATCGATCGTTCATGAAGTATCTACATTATTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCAACTTTTTGACCATCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCTTTGTTAGTAAGATTTGTGTTATTAAAGATGTTGGTTTCTGATTGTTTATCAACATCTTTGGCTTCATTTGATTTAATCAAATTATCTAGTTGTTGTTTCTTTGAATCAAAGTTTTGTTTAGCATTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCATTAACAATTTGATCGATCGTTCATGAAGTATCTACATTATTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCAACTTTTTGACCATCTTGTGAATTAATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTAGTTAATGATTTAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCTTTGTTAGTAAGATTTGTGTTATTAAAGATGTTGGTTTCTGATTGTTTATCAACATCTTTGGCTTCATTTGATTTAATCAAATTATCTAGTTGTTGTTTCTTTGAATCAAAGTTTTGTTTAGCATTTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCATTTGTTGCATTAACAATTTGATCGATCGTTGATGAAGTATCTACATTATTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCGACTTTTTGTGCGTCTTGTGAATTGATTAAGTCTTCTAATTGCTTTTTAGCGTCATTAAAATCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTGGTCAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTTGGTTTCTGATTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTATCTAGTTGTTGTTTCTTTGAATTAAAGTCTTGTGTGGCTTTTTCTTTAGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATTAATAATTTGATCAGTCGTTGATGAAGTATCTACATTATTGTTTTGCAATGATTGATTTGCTTTAGATGTGTCAACTTTTTGTGCATCTTGTGATTTAATTAAGTCTTCTAATTGCTTTTTGGCATCATTAAAGTCTTTAAGAAGATTGTCTTTTTGGTTCTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATAGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGTAAGATTTGTATTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACTTCTTTGGCTTCATTTGATTTAATTAAATTATCTAATTGTTGCTTCTTTGAATTAAAGTCTTGTTTGGCTTGTTCTTTGGCTGCATCAATTAATTTTTGCAAATCTTGTGTGGCTTTTTTAATTTCAGTTGTTGCATTAATAATTTGATCAGTCGTTGATGAGGTATCTACATTGTTGTTTTGCAATGATTGATTTGCTTTAGAAGTGTCGACTTTTTGTGCGTCTTGTGAATTGATTAAGTCTTCTAATTGCTTTTTAGCATCATTAAAATCTTTAAGAAGATTGTCTTTTTTATCATTAATTTTCTTAGTTAATGATTCAATAGCATCTTCAATGGTTTTTGTTTTTGATTCAATGTCTTTAATTAAATCATTACCAGCAAGATTTGTATTGTTAAAGATGTCAGTTTCTGGTTGTTTATCAACATCTTTGGCTTCATTTGATTTAATTAAATTATCTAATTTAGATTTTGATCTATTAAATTTTTCCTTTAATTTGTCAATATTATCTTCTTGTTCATATTGTCTTCTTTTAAATTTACTTTTTAGTGATGTTGCAACTAAGGTTGCCGTTGTTGCTAGAGCAATAATTAACGAAGTAGAAAGTAAAATTCTTAATTTCGATTTTTTCATATATCCCCTTTTTTTATTTTTCCCTTATTTTTTATATAAAAACTTATAAACAATTATTAAATTCTTATATTGTGATAATTTTCAAACAAGACTTCGAAAAAAAAAAAAAAAAAACGGTTTTCGCTCTATTACAATTTTTTTATGCACCTTTTAGTTTATTTTAATTGCTTTCTGTTAAAACATGCTTAAAATAAACTAAAAAATCAAACTCATATTTTTTAGTTGAGTTTGATTTTTATTTGTTTTGGATACGTTATTAATCTTGTTGAATATCTTTATTTGAACCTATATAAGCAAAAATTTCAGGATTTTGTTTTAAATATCTTCCTTCCAATATTCTTATAACGCTTTCAACTGTTTCGCTTAATGGCACAAATTGACCAGGCGATTTAGTAAAATGTTCAGTCATAAAGAAATTTTGGGTAAAAAACATTTCAAGTTGAAGAGCTTTTTTAACAGTGATTTTGCTTTCTTCATCAAGCTCATCAAATCCTAATATCAGAATAACATCTTCAAGATCTTTATATGCTTTTAATATTCTTTTTGCTTCAATTATTGCATCATAGTGACGCTTGCCAATAATGGTTGGATTAATTGCGTTTGTAGATGAAGCAAGTGGATCAAAGGCAGGAAAAATGTTTTTAGCTGCTTGAGATCTAGATAAGACCAAATTACCATCTAAGTGATTAAATACAGCTACTGCAGAAGGATCGCTTAAATCATCCATTGGTAGAAATATTGTTTGGAATGAAGTTATTGAGCCCTTCTTGTTTCTAAACAATCTGCTTTCAACTTCAGTTACATCACTTTCTAAAGTTGATTGATAACCTCCAATTGAAGGCTTTTTGCCTAATGCTGAACTTACTTCATTTTCTGCTTGAATAAAACGATAAATATTGTCAATAAATAATAAAACATTTTCATTGCATGTGTCTCTTAAATATTCTGCAGCAGTAATTCCGATTGGAACAATTGACGTTCTAGCTCCTGGTGCTTCATTCATTTTTGAAATAAACATTATTGAGTCCTTCATTAAATTAGATGAAGAAAGTTCATTGTATAATTCTATTCCTTCTCTTGAACGTTCACCCGAACCAATAAAGATATTAGAATTTGCTTCATGTTGTTTATTAAGGTTAAAAATAATTTCCTTCATTAGAACAGTTTTTCCAACTCCGGCTCCTCCAAATAAACCGATTTTTGCACCCTTAACAATTGGCATAAAGAAATCAATTGCCTTTATTCCAGTTTCTATAACTTCTTTGTTATTTTCTAGATATCTTTCATTGTTTATAGTTGAATTCATTTCTACATATTCAATTTTATTCTTTGTTTCTAGTAAAGGTTGACCTCAAAAGTTGTAAATATTGTTTTTTGAATTTTGCCCTATTGGAACCAAAAAACTCTTATGAGTGTTAATAACTTCTTCGTTAATTGAAATATCTTTACTTGCATATACAACAATTGCTCTAACATGTTTTTCGTCAATTAATCTTTTTACTAGTAAAAAGGTTTTTCCTTCATGCAATGTCAACAAGTGATTGATAGCAGGTAAATCTTTATTAAATTCTACTTCAACTATATCTGATCAAATTTTTATAATTTTTCCATTCATTATTTATCACCTAAAACCTTCGCTGCCTTATCTAATTGTTCTTCAGGTATTGGCACAAAATTATGTTCCAATTCAATATCTCAATTTAAATTTAAATAGTCTGAATATGATTTCAAGACAAATGCAAAATAATTCTTCATAATATTATCATCGTAGTCTAAATTATTTAAAATTGTTTCAAATGCTTTTATGCAATCGTTATAATTATTTATTAAATAATCTAGTAACATAAATGCTTTTTGTTCATCTTTTATTCCTTGCAAAATACCTCAAGAAATTAATTTTGTTGTTAGCACTACAAAACGATATGAGTAAAGTGAAAAACCCTTTTGCAAGAACATTTTGTCAATTAATTTTCCTTTGTGAAGCAAAATAGAAGTTTCTTTATTAAGCTCGTAATCTAACATTGCCAATTTTAAATGTCTCTTGTAACTTCTAAAAATTTTATTAATTTCACCCGCTATTTTGGTCATCATTCTTGATTGAACACTTGAACCTGTTCTAGAAACAGACAAATCAATGTTTATAGCAGGTAATTTTCCAGAAGCAAATAAGTCAGAAGAAGTTACAATTTGACCATCAGTTATTGAAATTATATTAGATGAAATTAAACTAGTTATATCACCGTCAACTGTTTGCAAAATTGGAAGTGCAGTAATTGTTTTTCTATTAACAAAAGATCCAGCTCTTTCAAGCAATGATGAGTGGGCAAAAAAGATGTCGCTAGGCATTGCTTCTTTTCCAACTGACTTGTTGGTTAATAGGGCAATTTCTCTAATAATATTAGCGTGTTTAGTTAAATCATCAAATACTATTAAAACATCATTGTCATATGAAAGATTTTCTGCATGAGCCATTCCAATGTAAGGTGCTAGATATTGTTCATAAACGTTTTGAGGCGCATCAATAATCATAGTGTTTTTCAATGCATCGTATTTTTTTAATGTTTCATAAATATTAGTTAATGATTCACGCTTTTGGCCAATTGCAACATAAATACATTTTGTGCCATTTTTTGCTTGATTTATAATTGCATTTAATGCCATATGAGTTTTTCCTGTTTGCCTATCGCCAATAATTAATTCACGTTGACCTTTTCCAATTGGAATTAATAAATCTATTGCAATAATTCCAGTATAAAGTTGTTCATTTAATGTTTTAACTGTCATCAAATTATGAGCTAGACCGAATATTGGATGATGATTGCTTAACATTGAATTATTTGGTCTCTTTTTTTCATTTGTTGGTCAAATAATGCCACCGTTAATATCAATAATTTTGCCCAAATAATCAAGACTAGTATTTATATTTTGGGATTCATTTAATTCAATTAATTCACTATTTATATCTAAAGATACGTGTTCGCTATTAATCAACATAAATGCCTTATTAGTTGTAGCGGAAATAACTATGAATTTAACATTTGGGTTACTTGCTAAATAAAATTGTTGATTTTGGCGGTAGTTATATTCTCCCTCAATTGTTGCTATGCAATTGAATATTGATTTAATACGAGGATTTTTAGTTGTGTTGTTCATGTGTTCTCCTTCTAACTATATAAATAATAGCCCTCCAATTATTAATATTAGTAATGCAATCGCTAGTATTACACTATTGATAATAATTACCTTTTTCTTTAATTTTAAGTTTTTTAACAATGCAAAAATTATTATGTTTGTAATTATTATGATTAAAGAAATAACTATAAAAGATGTCCCTAGTTTTTTAATAATCTTCTTTTGTTCAAGATAATTTTTATTTAAAACATTGACTGCTTCTAAATAAGCTTTATTGAAATTTTCTTTTTCTTCTTTATTAGCTTTTATTGAAATATTACTTGCTAAATTTGATAATAAGTCAAAATTATGTTTTATACCAGACAATAGTTCAAGGTAATCAGAATATCATTTCTCTTGGTTGAAATCTCTTTGGCTAATTAAAGCGGTAAATTTGTAGTTTAATTGTTTATTTAATAAGAATAATTCTCTTAAAACATTCTCATTGCCTTTGATTTCTGCTAAATTTGTTTTAATGAATTTTTCATTGAATTTAAGTGCTTCACTAAATTGTAATTGTACAAGATTATATGCCTTTGCTATTGTAGTTCAAAAGTATATATTATTTACTACTAGACTATTTAAATAAGAAAAAAATGTATAACGAATTGTTTTGCCAAACTCTTCAAGTAATTTTTTTGTATTTGTTTTATCTTCTTGCAATTTAACTCAATATTTTTGCAATATTTTATTGCTAATTGCACTATATGAATCTTTATCAGCTTTAAATGACATTTGTAGCGCTGAGTCAATCATTGCAAGCAATGCTTTTCTTAAATATCCATTTCTTAGCTTATTGTAATCTAATTTTTCGTCTAAGCCCAAACCTTTATATAATGGCTTAAATATTTCTGAAATTGATTTTATTTCAGTTTCTTTTAAGAGATTAAAATATTTGTCTTGTGACGGAGTATATTTTTCAATTAAATATGTTCTTTCTAACTTAGGATTTATATTATCTGAAATATGTACTTTTAAATTAATAATATCATTGCCATTAATATCAAATGAAAAAACAGAATATTTATAACGAGTATTTATTGGGTTATTGAAATAAAATAATTCTTGACGTTTGCTTGCATCTAATGAATCAAATTGGCTTTTAATTTGACTTAATGATGAAGGCAAATTATTACTTGATATAAACGGAACCAAAAGGGGCAATGATTGAATTAAATTTTTTTCTTGTTCTTTTGTTAAGTGACTTGTTTTATCACCATCAACCAATGGTTTTAAATCAGGCAAGGAATCAGGATTTTTTTCTTCTTTTTGTTCAGCTTCGTTTGTTTCTTGATTTTGTTTTAAATCAAACTTTGTAATTCTTACTTTAAACTTTGAAATAAAATATTCATCTCAACTTTTAAAGCCTTTGGGCAAAGTGGTAGAAAATACGCCGTTTTTATCCCTAATTAATTCAACATCTTTGTCAATATTTAAAACATCTTTTTCATTAAAAAATAACTTATTAACTTCTTTTAAGAAATCAGATTTATATTTCTTTAAAAATTGAGAATACTTATCTTTTTTAATAGTATTTAATTGATCTTTAGCAACCTCTTTAACACTTCCTTTTGGCAAAATTGCTTTTTTATAATCTAGTGGATCGGTTTTACCAGTTTTTATTGATTTAAATAAATCGCCTTCGTAATCAATATCCTGCAAATCGTGGTTTTTTTCTTTACCTAATATAAGGGGAGTATTTAAATAAATTCCATACTTATTAGGATCTTTTTGAATTTCTTCTTTATTTTTTGTTAGAAATGTTTCTAAATTTTTTAAATAAATTAATTGTTGAATTTTTAATTTAAATTCTAATTCTTTATTTTCTAGCAATCTTTGTTGTTGAACTTGCAAATAATTGATTATTGCTTTTAAACCATCTTCTATTGTTGAAGAAATTGTTTTTTTAGCTTCGCTTTCAAATTCACCAAAATTTGGATCTAACTCAGTTTTATTATTTGGATCATTATTTGACGCTGCCGAAACTACTATTGGAGATAATAAAGGAACTGTTGCAAATGATAATAATAAGATTTTCTTTTTATTCATTGTTAATCCTATCTTTTTCAATTAGATTTAATTCTTGCCTCAAAGATAACAAGTAATATTTTTCTTCAAGAACTTCTAATTTTAAAAATTCATCTAATTTTAAACCTAATTGTTGTTTGGCCTTTCAAAATTTAATCTCGTTTGAAAGTTCAGATATTGTTTTAACTAAATTGACCTTGTTGAGCGATTTTATATAAAAATGAGGTTGGGTTAATGTTGAAATTGTAGCTATGTTATTGTTTACAACAATATTTGAGTTTTTTAAAAAAACAAAGAAGGTTTCGTTTTGTTTTGAAATATCAATAATTTTTAGCAAAAGGTTTTCATAAGAAGCAATTGATGTATTTGCTATTTGTTCTCATGTATTTTCTTCATTGACATTAATATAAACTAAGCCTTTTTCAATATTTAGCTTCTTATTTTCTAAAAAAAGTAATTCAATTTTATATACGTTATTCATAAATATCACCTTCATCAGGGTCTTTTTTAGTCAATAAATTTAGTTCCTCAATTTGCAATTCACTTTTTGCTCTTGATATTTGCTTTTTAAGTTTAAGAATGCTTTCATCTAAATCTTTTAAAATTGTATTAGTTGAAACTAAACCTATTTTTGCTTTATAAAATGCAGATTCAGATATTAGCATATTTATAGTGTTTAATAAATAACAATTAATTTGAGAATTAATAAAATCATTAATATTGGGATATATTTTTGCTTTTTTAAAATCTATGTCATATAAATTATTATTGCTTTCATTTAAAGGAAATTTTTCAAACGAGAATTTAGACATTGGCAAAATTGTAAAATATTGATTAAAATTTTTATTGGAGTTAACTACAAAATTGACTTCTTCGTAATTTTCATATTCATACAAGATCATTACTATTTTGGTTAATTTTTCTGCCAAATTTTCAATATTTGAATTTTCATAAGATTGCAATACTTGTAGGTTATTATCTTGACCAAATTTAATTGCATTTTTGCCAATTAAAATGAACTCATTATTAGATTTTTTTATATTACTTAGAATAGTTTTTTCATATCTTGAATATGAGTCAGTGCTATATTTTTGTTCTTCTGTTAAATAAATTCACAATATGTTTCTATTTATTTTCCTAAATAAATTAAACCTATGGCGACTTTCTCTCCTCAACATATCATTCTTAATATTGTAGATTTCTTTTAAATGCAAAATTATTTGCCTTGATACCAATGCATTTTTATTAAAAAAGCTTAATCTTTGGTTAAGTTTCATAATATTTATTAAATAAATATTTTTTTCATTATTTACTTTTAATGAAATATTGTTTAAACTTTTGCGTTTATTCTCTAAATCTTTTAGTTGCATGTTATTGTTCCTTTGTAACAACTGCTACTACTTTTTCACTGAATAACAAAACTAAATCATTTTTTAATTCTGAATGAAATAAAATTATATGTGGAAATATTTTAATTGATTTTTTAAGAGCCAAAAGTTTTTTTATTTCAGCATTATATGAATTCAAAAATTCTTGGTATTTTATATCGCTAGAAGCAGTTAAATCAATTGCGTTATTGGCAGAATAATTTTTTGTTGTAATTATAGGCACCAATACCTCTTGTGAGAATTTCAAATTCAAATTAAAAGTAATGTTAAAATCCTTTAGCAAAATGTCGTATTTATTTTTAAAAGCTAAATCAATTTTGCTTATAAAATCTAAATAAATAGGAGAATTAAATCCTAGGTTGTTGTTAAATAATACTGTTGATATCATTTCATCAAAAGTAATTAAATCTTGTGAGGACTCGTGTTCTTTATATTCTTTAAATACATCAAAATAACTCAATAATGGATTATTGTCTTTCTTTTTCATATTCATTCTCCTTATTTAATTTTCTTATATAATAAATTGCCAAAATAAATTTAAGCAATAAAGATAATGCCATAATAAATGCAATGCTAAAGTTTAAAAGTAAAATAATTTGAATAATATTCATTCTACTTTTAATTATTGAAAATAAATAATTTTCATTGCCATCAGCAGTTAAAATATAATTAAATCCAATAATTGCAAAGATTAAAATTGAAGAGATTATTGCTAGTTTAATTATTATTAATAATAGCAATGAAATATTCTTGTTTTGTTTAGCAAACACAAATAATATTACTATTTGTGTTGTTGCTGTTACAGCAACGTTGATTAAAGAAACAATGCTATTGGTGTTAAATAATGTAGTCAATAGCAAACTTAACGCAAGCGCTATTTGAACAATTGCTAAATTCAATCACGATCTAAATGAACTTTTCATTTCAAAACGCTTTTGAAATGAAACAACCATATATGCAACAAATACCACAATTGCAAAAACGCTTTGAAAAATGTAAATTCATGTTGTTTTTGAAGAGCCTATTGAAATGTTTTCATTATTATGCTTATATGCAAAAATTCTTAGAATTCAAATAAATACAGCCCCAAATAACACTAATAAAAATACTAATTGATTTTTTAAATAGAATTTATCATATTTTTTATTTATCAATAAAGCAAAGAATTTAATATTAGATAATACTATAAAGGTAATTAAAACTAAAAATGTTAATACAATAATCACAAAATTGCTAGCAGATTTAATTGTTAAAATATCTCTAATTGTTTCATAAAATTTATTGCCTACAAACAATATGTCTTTATTAATTCCTAAATTAATTCAAACAAATGATATTGCAACAAATAATATTGTTAAAATCCCTTGAAATGTTAAAGAAATTATTAAAATATTTTTATTTTTATAAAGCAATGGATTTGTTTTTCTTTGAATTAAAAAGTTAAATATTTCATAGCTAAAATTAATAATATATAAAGCAATCAAAATAAATGATATTGCAAATAATTTGCTTGGAAGAATTTTAAAATAGCTAAAAAATAATGTATAAGAAGTTAATGAAAGCAATAAATATAAGAAGTAAAATGGAATATATTTTTTAATAGTAACCTTATGTCAATATAAATTAGCAAAACCTTTTACTAAACTATAAAATAAGACAAAGGCTAAAATTAAGAATCTATAAATTATTAAAAAGTTTAATTGTTGATTTCTTGGACTTGAAAAGTCAAAAAATGAATTTAGTGAATTAGAAAGCAACATTTTATTTGCAAAAAGTGTCTTTTCACTAAAAATTAATAAAGTTGTAATAAATACAAGACTAATTGCAATCAAAAGGTATTTCAACACCTTATTTAAAATGTTGTTTTTCTGCTGATTTTTTATTGAATATTCATCATCATAAATCAACTTTTGATTTATATTATTCGAATCGATTTGTTTTTGTTTCATCTTATTATTTTCCTATTAAATAAAATCTCCGTCAGAATCAGAAGAAGAAGAGGTCTTTACACTATATCCCAAACTTTCTAATTGAGATTTTAAAGCTGAGGCATTATTATCAACAATTATTTCTTTTTTCAATTGTTCTGCAAACATATATAAACGCGAAAGGTTGTCAATTCCTTCTGAATTTAGTAATGAAGTTCCTGTGATTCTTACTTTTGTTGTTAAATTTCCATTACTAAATAAAATCTTGCTTTTTTGACCAGGTTCGCCCGAAACAATATGTCTATTAAAACCGGCTTCATTTAATTCTTCACCTGATATGTTGAACGCTTCACTGTTATTATATAAAGTAAGTCTTCTTATCATTCTTGATTTTGAATTACTTGGTTTTAATTTATCATGAAATTCTAATCCCCTCAGAGAACGTATCTTTGGTATTCTAGTAAAATCTAGGCCTTGTGGATAACTATTGCCATCTTCTTTATGATCTGGTTCTAAGCCCGGACCAAATCCACCTTCAAATATTGGTTCATTATTTCTAACTCAATAAACCATTCTCATTCCATCGTTAATTCTCTTTAATTTTTCTTGTAAAGTAGAAGCATTTTCATTGTAATCAACAGGATCAAAGGCCAATGTATCAAATGTTATTCTTGTGGCGATATCAGCACCTTGTTTATAGTCTCAAGAAACATTGTAATCGTTTGAGTTTATTCATTCAACTTTTCTTAATGCATTAGGGTTAATTGATCATTTATCCAATAATGAATTTCCTAAAGTATAAATTCCTAGTTCCTTTATGTGTTTATTTTCTAACGCAATTAAAGAAGAAGTGTTTGAAGAGCCCGCTGAGAAAAATAGTTCTAGTAGTTGAATCTCATCAGGAATTGCCTTTAAAATATCCATAAATTTTTGGCTAGAGTCAGATTTTCCCATATTTCTAATTCTATAACCAGTAATATTGACCTTTTTAGCCTTTAAATCTTCAATTAATTTTTTAGTTTTTGCATAACCATCTGGGTTGTCAGCATCAATATCAACGATATAACCTTCATTGATCTTTCCGTCTTCTTTTTTTTCTCTAGTATATTTATTGACAGTTATACCATCACCATCACTAATACCATATTTTTGAAATTCTTTAGTTACATTAGTTTTCTTTCAACCAGGATAGTCGCCATTAGCAAGACCATATGGGCTTCTATTTTCAATACTATCATATCCAAAAACTCTTCTATTTTTATTGTCGTTTATTATTCTATTTGCAACACCATTATAGCCTTTGGCTGGAGAATATGCATATGAATCTAGCTCGCCTTTTTCGTTTACATAGGCGTTGTCTTGATCTAATGCAAGGCCTTCAGCTAATTGCTTTTCAGCATTAGAAGTAATCTTTGTAAATTTAGAGAAATCTAAATGAGAATACAATCAAATATATTTGAAGTGTTTCCTGCCTTCAAATTTAGGCTTCATTTCTGGATATTTTGCCTTGCCTTCTGCTGTTAAGAAATTAACAACATTTTCTGAATCAAATAATCTTTGGAACTTTAATAATTGGTTTGCAGTGTAACTTAATGAACCACGTTCAAATTCTTTTTTAAGATATTTTTCGGCGTCATCTATTCAAGTTGCCCCTCAACTTTTTTCATAATCTTCAAGATCTTTTTTGAAGTATTTTTCATCTCTTTTTAAACGTTGAGTAGCCGCTTGGGCTGCTGCTTGTTTCAATTCTTCAGTAACCTTAACTTTTTTAATTTTTGGAGCACTATCATTGATATATGGGTTTAAATTAGTTAGTTGATTTAAGATATCATATTGATTATATTCACGTTTCTTTTGATCTTCAACTTCCGCTTCAACTTTGACACCGTTTAGAATAATTTCAGTTGTTCTAGTTGAAGGTTTTGATGAAGGAGGAGTAGGCTTAGGTTCGGGTTTTGGCTTTGGCTCTGGTTTTGGAGCTAGAGGGGTTGGTTGAGGTTGAGGTTTTGGTTCTGGTTTGGGTTCGGGTTTTGGAGCCGGCGGAACTGGGATTGGTTCAAGCTCTGGTTTAGGGGTTGGAATTGGTTGTGGCTTTTCTAATTCTTTTAAGTTGTTATCCTTTATTGAGGGCAACGCATTTTTATAATCTAGGTTGCTTTTATTTATGAAATCATTTCCTGTTGCTGAGCTGGTGGTATTAAAACGAGAATAATTTTGCGAACTTGTACTATAAAATATAGCCCCAGCAATTATTGAAGCTCCTATAATAACACCTGCCGTCATTAAAATTAATTTGTTTTTTCTTTTCTTTAATAAGTTTTTCATTTCACCACTTTTTTTAAGTTTTATTATTGATTATTTTTTATTTTCTATAAATTTAAATTCATTAGGAACTTTATCATCACCTAAGCCATTAGGGAATAATGCTGTTCTAAAATCGCTATTATTTTTATATAGTTCTTTTAATGTTTGTCTAAATGAATTTTTTTGGTCTTTACCGCCGCCATAAATTAAATCATATTGAGGCAAATTGTAATTTCCATACAAGCCTTTGTAGTCAAATCCTTCCGATCTAAAAGCAGCTGCTAAACCGGTTTTTGCGCCTATATTTGATAAATAAAATATTCCAACTAGTTCGTTATTTTGATTTCTTAAGCTTGTGCCAGAAGCGCCTCCAACTGGAGCAAAATGTTTAGGCATATAATTTAGCCCCATACGCATATATGTTTTGCCATCGCTACTTTCATAGAATGAAAATACTGATTTTTGATATTTTTGGCCGTCTTTTTCTTCTTGAACTTGCTTCATTCCACCTGGTTGTGCTAAAAACACATCAGAAATACCAGGTTTATCAATGAATGTTCTATATCCTAAACTATAAGATAAGTAATTTCCTCTTTCTGTTTTTGATGGAGGAAATGAAGGGGCTTCATTTTCGCCAACTGCTAATTGATCATAAAATCTATAATCCGAATTTGTTCATAAACTGTAATTAAATTCATCTCTTCTTTTAATTTGGTCATCATCTACATATCTTCTTAAAAAGAAATCTTGGGTTGAATTTGGATAACCCAATGCAAAAAGTTCATCATAATTCTTTCATCATTCTTTGTTTTCTTTATTTGCCTTTAAAGGAACATCAATTTTTTGGTAATTTGATAAATATGATTTCGACAAGAATTTAATTTGTTTTTCTTTATTTTGAGCATAATTACTTGTTAAGGCTCTTGCTAATTCGTGGGCATCTAAATTGCTAAATTTTTGTGTAATATCGCTATTTTCGCTTAGATCATTTTTAGAAACGCTTTGTAATGTCAATTTTTCAAAATCAACTTCAAAAACTGCAAAATCAGCATATTCTTCAACATCTTTATACTTTTCTTTTTGCGATTTAATTAAATAATCAGAAGGTTTTGAATTTAAGAAATCTCTTGCAGTATAAACTGTCTTTATTCCATTAGAAACATTAGTAGAATCTTTACCAACAAATGAAAATCTATATGTATTTTCATCAAGATTTGCCAAACGCAATGTTGTTTTAATTTTTGCACTATCCATCAATTTTAGAAGTGTAAATGAAGTTGTAAGTTGCTTTAATGCATCTGCAACGTGTAAATTTGTTGCAAAATATCATTTTGTTGGATATTTTCCATCACTTCTTTGTTGATAATCCATAATTCAAGTTGTTCCAGCTGTATTAGTAAATTTTCTTTCTTGGCCTTCAATAGGAGTTAAGAATCTTATTCCAAATGTTTGTGAAGCAATGTCACGATAATGTTCATTCGGCAATGTTCTTGCTAAACCTGTAATTTTATATGGATCTCTACCAAATGCATCTGTAGGTGAAGGCCCTTGACCTCCTGGAGTTTCATTAATAGCAAGGCCTTCTACATTGCCTTTTGAATCGTAGCTTGGCAATGTAAAACCTTTTCTTGCAGCTGATTCATATGAATCTTGATTAATTTCTTTTGCCTTAGCATCAAATTTTTGAATGTTAGATTGTGAAGCTTCCAAATTAGGTCTGATTTCTTTTATTGATTTTTGTTCTTGTTGACGAGTTAAAATGTCTAAATATTTTTTATTATCTAACTCATATCTTTTTGTTTGATTTCCTTCAAGATATTGTTCCAAATCACTTTTATTCAATCTTAAAGCGTCAGAACCTGTATTAGGAATTGTTCCATTTTCATCAAAACCATAAGGATTTGTTTTAAATTTTCCTACAGTTATTGTTTCAATAAATGTTTTGTTTGTCTTTTTGTCTTTAATAGTAATATTAAAATTCAATTGACCGGTTTTATTTGCAACTGATCAATCATCAGAATTGGTTGAAGTTTGTACGTATAAAACATTGACTATTTTTTTAATTTCAATTTCATCATTAAATTTTTTTTGTTTAAATTCAACACCTTGATTGAAATTTCTAACAATTTGAGATGGAAGCAAATCTTGTGAATTTTGATTTGAAAAAGCCTTGAATTTCAAATCAAATAATTGACTTGGATCATTTAATGCTAATTTTGAAACCTCAGAAATTATTTTCTTATATTCGGTAGTTTTTTGTTCTTCAACTGATAGTTCATCTGTAGAATTATTATTTTTGTTGTCGTTGCCTTTTGAATCTTGATTATTTTGGGGTTTTTCATCAGGATTCTTCTTTTCGTCATTTTGATTATTATTAGTCTTTTTATTTGTATTTTTACATGAAATAGCTACAGCAGACATACTAGAAAGTGATGTTGCCGCTATTAATAATGATATTATTAATTTAGATTTTTTCAACCTTATACCTTCCTTGTTTTTTAATTTTAAACTTGAAAAATAGGAACTAAATTTCCTTTGATTTTTTATTGATTTCCCTATCTTAAAAAAACAAATATTTCCATTTTTCGCAATAATTTTACTATATTAAATTTAAAAAAAAAAAAAAAATACTTCAATGTTTAAAAAAATTACTTTAAACTTTTTAAAATCAATATTAAATTCTAGGGATCTAATTAAAATTAAGATAATAAATGCATATCAAGATATGCTTTAAAAAATGAAATTTTTCATTTTAATGTATAATTTAAAAATTAATATTTGAGGAGGCAACATGTTGGATGTTTGCAATTTAGCTAAAGTTTTCCCAGACAAAAAATTATTTACAAATGTTAACTTAAAATTCTTGCCTGGAAATGTATATGGAATTATTGGCGCTAACGGGGTTGGAAAATCCACTTTTTTAAAAATATTAAGTGGGGATATTGAAGCATCAGAAGGGCAAATAATTAAAGAAAAAAATGCTAGAATGAGTGTATTATCACAAAACCAAAATGAATTCGACGATTATATTGCAACTGATGTTGTAATAATGGGGAATAAAGAACTTTATCAAATTAATCTTGAAAAAACTAAAATTTATGAAGATCCAAATGCAACTATTCAAGATTATGAAAAAGCAAGCCATTTAGAAGAAAAATTTGGTGAAATGGGGGGTTGAAATGCTGAAAACGACGCCCAAACATTATTATCAAATTTAGGAATTGAACCTGAAAAATGAACTATGAAAATGTCGGATTTAAAAGCTAATGAAAAAGTAAAGGTTTTATTGGCAAAGGCTTTGTTTGGAAATCCTGATATTTTAGTAATGGATGAACCAACAAACAGATTGGATCTAAAAACAATTAAGTGATTAGAAAACTTTTTAATGGATTATCCAAATATTGTTATTGTTGTTTCTCACGACAGTGATTTCTTAGATGCAATTTGCACGCATATTATTGACATAGATTATTCTGAAGCTAAATTATTTATTGGAAATTATTCATTTTGAAAACAATCAAGCCAATTGTTAATAGAAATGCAACAAAAACAAAACCTTAAAAAAGAAGAAATTGCCCAAAAATTAAAAGATTTTATTGCCAGATTCTCTGCTAATGCCTCAAAATCAAAACAAGCTACAAGTAGAAAGAAATTACTTGATAAAATTGTCATTGATGAAATCAAACCATCATCTAGAAAATATCCTTTCATTCAATTTAACTTAAATAGAACTCCTGGAAAACAAATATTAACTGTTAAAAATTTAACTTATGTAAATGACAATGGCGACACTTTATTTGAAAATGTAAGTTTTACAATCCACCCCGGAGAAAAAATGGTTATTATTGGCAATGACGACATTGCCAAAACTAGATTGCTTGAAATACTTATGGGTAAGCGTAAACCAACATCAGGAACAGTTGAATGAGGAATAACTATTAAACCAAATTACTTCCCAAATAATAACCTAGAATATTTCAAAGAGCCAATGTCAATAATGGATTGAATTAGCAAATGGCCTCTAAATAATACAACTCAAGAAACGAAAGATAATAGTGATTCAAGAATGAGAGCATTTTTAGGAAGAATGTTATTTTCAAATGATAGCGTATTTAAGAATGTTAATGTAACTAGCGGTGGTGAAAAAGTTAGATTAATGATGGCTAAACTAATGTTAGAAGAAAGTAACTTTTTAATCTTCGACCAACCTCTTGACCACCTTGATAGTGAATCAATTGATAGTTTAATTGAAGCAATTAAAAATTACAAAAGTAGTTGTATATTCACAACATACAATAGAGCAATGATAAAAGAATGTGCCTCAGTTATTTTAGAAATAAAACCTACTTCAAGTTATTTATTCTATGGAACCTTAGAAGAATATGAAGAAGCAATGGGATATTAAATAACTAATTAAAAAAACTCGTAATTAAAACTACGAGTTTTTATTTACTTTTTAAAGTTTAGCAGCTTATTTCTATAATATTCGTATTGTTTTCTACGTTGATCAATTTCAAGAGGAAGGCCAGATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGGATTTCAACTATTTTGTTTTGAATGGAAATGTGAGGGATTGGAATAACAAAATTTTGTAACATTGGAAGAGTAATATAAGGAATTCCGGAATCTAATTTATAAACATTAATATAATTTTTTAAGTTACTCTTTAAATAAAAGTAAGCATATTTTCAAAGAACAAAATGATTTATTTTTAACAAAATATACGTTCTTTGGTATGCATTAAATTTTCCATTATAAATGTTTATATGACCTACTTTGCTTCCATTTCCTGATATTAATATTGACATTTCATCAAAGGCATATTCATTTATTAATTTTGGTCTTTCATCGCATGTTCAAAACACATATTTTCCATTTGGAGTTTCTGCATTTGCGTTTAATTTTCCGGTTTTTATTTCTCTAAAAATTTTTGATATAGGTAAGTATTCAACTATATTGACAATTTTGTCTCAAAGTTCATTTAAAAGCAGTAAATAATTTTTACCTAATACCCCCCGCAAGGTCTTTGAAGTCTAACAACTTATCTCTATAATATTCGTATTGTTTTTTACGTTGGTCAATTTCAAGAGGAAGGCCAGATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGGATTTCAACTATTTTGTTTTGAATGGAAATATGAGGGATTGGGATTTCAATATTTTCCATAATATTACTCATAAGTTTAGGATTTCCACTTGCCTTGTTTACAAATTTTTTTGTCTGCATAGATAAGCAAGTGCTTAAATATTTCACATTTAATTTTGATTTATTTAGAACACTCAATATTCCACACACATTAGTTATTGAAAATCTACCAATTCTATAAAATACTGTCCCGGCATATGCTCCATCTGTAGTTCATGTTACATGTTCACCATCAAAATCATATTTGTCAATTTTTCCTAAAATTCCATCGTTTAAACTTTGAGATGAATAAACAGGATATTCACCTTTATTTAATTTTATGAATTCCTTAGAGTAAACTTTTCCTCGCTTTATTTCACACAAGTTTTTAATTAAATCATATTCAACTATATTGACAATTTTGCCTCAAAGTTCATTTAAAAGCAGTAAATAATTTTTACTTAATACCCCCCCCCGCAAGGTCTTTGAAGTCTAACAACTTATCTCTATAATATTCGTATTGTTTTTTACGTTGGTCAATTTCAAGAGGAAGGCCAGATTGAATGTCTTTGGTATATGTTTCAAGTTTGTCAAGGATTTCAACTATTTTATTTTGGATTTCAAGAGGGGGGAGTGGAATTTGCAATTCAATAATATTTTCCATATCAGGGTGTTTTATTGAGGAACCCCTAAAATTTTGTTCAATGTTTTCTTTGTTAGCAATTAAAAAATAATATAAGAATTTGAGACTATATTTTTTAACATCTTTTGATGATAATAATATATTTAAACTATCAATAAAATATCCTTTGTAGTATTTTATTGATGGCACGCCACCAGATGGTATTGAAATAATCTCACCATAATTAATGTTTTTATCATTTTCAACATAGGAGGTAAAACCATCATATTGACCTGTTGATAATAATTTTACGTTTCCCGTTTCTTCTTTTTTATAGAGCTTAAGTTCATTTGCGGAAACATGCTTAAATGACAATATATTTTGTTGTTTGTTTTTATCTACTCCTTTAAATTTTTTGTCAAAATCAACAATTTCTCATAACCTCTTATATTCAACTATACTATTATTGCATTGTTGATTATTCATTATCTTGTTTAATAATTCTTTATTATTATCAAAGTCTAGTAATTTATTTCTATAGTAATTATATTGTTTATCTCTATAGGTCAATTCGGTGGTCAATTCGGTGGTCAATTCGGTGGTCAATTCGGTGAACTTGTCTAAAATATTAACAATTTTATTTTGAATTTCAAGAGGGGGAAGCGGAATTTGAATATTTAAAAAATCGGATTTAGGAATATAAAATCTAGTCATTCCTTTAGCAATTTTATTAACCTTTTTTCTAAAATCAACACAATGAAAATAAAAATTTAAATATATAGGATTAATCTCTTTTAAAAAATCTTTTAAAATTCTTATAGCAAATAAATGATCATCCATAAATATTTTATCCTCAATATAATCATCTATAATCGAAGATATGCAACATTCGTCAGGGGTTTCTGATGAAGCCGTAAACAAGATATCGTTTTTTTGCAATATATTTTGATTTAATGATTTTACTGTGGCATTTTTTAGTAAAGATACATCAATTCTAAAATTTTTATAGCAATTCATATAATCTATAAATTTGCAATTCCCTTCTTCACTCCATTTTTTAGATACTCCCTTCATACCCGAAAACAATGTAGAAATTTCTCCAATTTTTTTATATTCAACTCCATTAGGACATAGTTCCTTAATTAACTTTTCTATTTTGTTCATAACTATTCCTCTAATTCAGCAACTATTTTGTCAATTTCGCTTCTCAATGTTTCAATCTTGGAAACTGTTTCTTTTATTTGAGAGTTTAAGACATTAATATCTATTTTTTCTGAAGTGTCTTGTTTTTCAACGTAACTATTAACTGATAAATTATATGAATTGGACTTAATTTCTTCAACACTGGCTAATTTTGAAATATAAGAAATGTCTTTTCTTTGGCCATAATAATCAATAATATTGTTAATATTTTGACTAGTTAGCTTGTTGTTGTTTGTTACTTTTAAAAATTCTTGAGAGGCGTCAATAAATAAGATATTGTTGTCATTTTTTGATTTTTTCAATACCATTATGCACGTTGAAATACTTGTTCCAAAAAACAAGTTGCTTGGGAGTTGAATTATTGCATCTATGAAGTTATTTTCAACTAAATATTGTCTAATTTTTTGTTCAGCGCCCCCACGATACATAATGCCCGGAAAGCAAACTATTGCTGCTACGCCATCAGTTGCAAGTCAAGAAAGGCTATGAAGTATAAAAGCAAAATCGGCCTTGCTTTTTGGAGCTAATATTCCAGCTGGCGAAAATCTTTGGTCATTTATTAATAAAGGGTTGTCTTCGCCTTCTCATTTAATTGAATAAGGAGGATTTGAAACGATAACATCAAATGGTTCTTTGCGTTGATGTTCAGGGCTTGGAGATAGCAATGTGTCGCCGTTGTAAATATTGAATTTGTCAAATCCTATATCATGCAAAAACATATTTATTCTACATAAGTTATATGTTGTTAAATTGACTTCTTGGCCATAAAATCCATCCTTGATATTTTCTTTGCCAAGAACCTTGATTGTTTGTAAAAGCAGAGAACCCGATCCACATGCAGGGTCATATACTCTATTAACTTTTTTCTTATTAAAAACTGCTATTTTAGTAAGTAATTCAGAAACTTCTTGGGGAGTAAAATATTCACCCCCTGATTTTCCGGCATTTGATGCATACATTGACATCAAATATTCATAGGCATCACCAAAGGCATCTATTGAATTATCTTGATAATTGCCTAATTTCATGGCTGCTATTTCTTGCAATATTGCTGCTAATTTTTTATTTCTTTCATCAACACTACGACCTAATTTTTGAGAATTAACATCAACGTCATTAAATAGACCTGAAAAATCATTTTCACTTTGTTGTCCTTTTGCTGATGATTCAATATTTTTAAAAATATTGTCCAACGTTTCATTTAAACAATTGTTTGTTGAAGCATTTTTAACAACATTAATAAACAATTCAGAAGGTAAAATAAAAAATCCTTTTTCATTGATTAAATCATCTTTGCCCGTCAATGCTTCTTCATCTGATAAAGTTGTATAATCAAAATCTTCAATTCCTGCTTGACGTTGGCGATTGTTTGCATATGTTGCAATATTTTCAGAAATATATCTATAAAACATAATTCCTAAAACATATTGTTTAAAATCCCAACCATCAATTGAACCGCGTAATCTATTTGCTATATCTCAAATTTTTTTATGTAATTCGTCACGTTCTTGATTATTAATAGCCATAATTTTTTCCCCCCCAGTGTAATAAATAACTTTTATATATAAATTTTACAAAAATAACCCCAAAAAAGGTTTATTATCACTTTAAACTTAAAAAACCTTTTGAATCTTAAATATACGATGACAATGTTTCTACAAATTAAAATACAAAAAAACTCGCAATAAATGCAAGTTTTTTTTAATATATCAAACATTTTTTTAATCAATAGGTTTTATTGCTTGAAGAACTAATTTAGCAAGTTGATTGCCACCTAAAACTTCAATAACAGCTAATGCAAAATCAAAAACAGCAGCCGCACATTTTCCTGTTATTAATTTCTTATCTGGACTTATTGATACTAATTCTTTACTACGATTAGATATTTCTTTTACACTGTCGTTTATTGGAAATGAACTAAATTTAGTTTTACTATCAATAAACTTATTTTCATATAAAACATTGGGAGCATCGCATATTGCTATAATTAATTTGTTTTGATTTTTAAACTTAGCTATTAAGTTTAAAAATGTTTGGTTCTTTCTAATACTTTTAGCAGCAGGTCCTCCAGGAATAAATAAAACATCATATTCATCAACATTTATATTTGAAGTTGTTTGCAATTTTACAATTTTATGTGCGCCAGTTGCTTCAATTCCTAGAGGATTATAAAATGTGGTCTTATTAAATAAATTATTTGCTCTATGAAATAAATCTAATGTTCCTGTTAATTCAGTATCTTCAAATCCTTCTTCAACAATTACTAATAAATTCATAATTTTCACCTCTTGTTTGATTATATACTTTTAAACAAAAAAGAATAGACAGTTTTAACTAAAAATCTTTTATTTTGTTAAATAAATTATTTTAAATTTAAAAAATTCAAGTGGAATATTTTTAACACTTGAATTTTTATATTTTTTAACATTTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGGTTTTGAATATTTTAAGCTGAAATCCATTTCAATTGTTTTAGGATTTGAATTCATATGAATTGTTAATTTATGAATATTAATATATAAACCAGGCAATTCTTTTCAATCTTTTGCAGCACCTAATTCAAAGTTTTTAATTGTGATATCGCCGCTTTTTAAATTTGCATAAATTTTAGTTTTAGAGTTTCGATATAATGAATTATATTTAAAGAATGCTTCTTTTAAATCAACTTTTTCATTATCAATAAATTTTGCTTCGAGTAAAACTTTTTTAGGAATCAAGAAAAAAATACAATTAAATTCTTTATCTTCTAAGGTGAATCAATCTAAAATTCAATAGTCTTTTCAATATTCAAATCGTTTTGACTTTGGTCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCCGCTATACTTTTATTGCTTTGCAATACTGAATTATATGATTTGGCACTGAATAAATTTTCTATATAAAAATGTGGATTACTATTTCCAATAATGTTTAAAACTTTTTTATGTTGTTTTTGTTCATTATAGCAAGATGTAACACATAATGGCAAAATTGGCAATGTAGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATCATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTATAAAAATCATAAGGATCTTTATTTAGTATCTTCTTCAAAAATAAGTTTAAATTTTGAATATGGATTAGTTCCAAAATTATATTTGCTTTTTGGGTGTTGAATATATCACAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGATTGCAATCTAATGTTAATTGTTTAATGTAAAAGCGCAACCTGGGTTCACTATTCTTATCATCTAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTTGAAATTTTTCTAGGCTGTTCATTAAACACGAAATTAAGGTTTTTACTATAAAAGTCTGCTAAATTAAAATCTTCGCTTGCAATAAATCCTGTTTCTAATAATACCTTTTTAGGAATTAAAAACAAAATGGAATATGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATAGTTGCGTCGTTATTTAAAACTGATTCTTGTTCTTTTAAAATATCAACCATATTAAAAACTATTTCTTTATTGCCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAGGTTACAAATAATGGCAATATTGGTAATGCAGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCGTTCCCGAATATTTAATTCCGTTTTTAATAAAATGCTTCCTTGTTTTTTTAATTTCGCCATTTGGTTTCATACCTAATAAGAACAATTGGCCAGAAAGACTCGAAGAAACAATAACTTGAGAATATCCTCCCCAACCATAATGGCAAAGAAGTTTATTTCCATTGTCATACATTCCATAAGCAACAATTGCATGACCAACTTTTCCTCTTCCCATCGGATCAGGCATATATGTCCCAAATATTAAACAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCTGCGGATCTTCGATAAACATAAACAGGTCAAAAATTTGGTAAATCTGGTTTTTTTAAAAAATCATAAGCAACGTTTTTTAAATATGATGAGGTTGTTACTATTGCTCCTCATCCGTGTTTTGTCCATAAATCTGTAACAAATTCATCAGAAATAGCAGGAGAATATGGATAGCCATATTTTTTAAATGTTGAATAATCCGATGAATCCTGATAATCTACAAAGTATTTTTTCACTTGGTCATCTGAAAAGACTCCATAATTTTGAAAAAACTCAGCATATAATAAAAGCATACCTAATGCAACATAATGGCAAAGTCCTTGTTCTGTATCAGAACATTTATATTTGTCTATTTTGTCTTGAATAAATTTTGCTGTTTCCTCATCTTTTCCAGCTTCCTCTTTTTCTTTTTCTCATCTTGCATTAATTGTAGAATAATCTTGATAACCAAAATTTTTGTCTTTTATTGCTAGCTTAAATCATCAAGAATACGGAACTTCTTTTTCTGCATGTATCATTTCAACATCAGTATTAATTTCATGAGCCTCGCTTGAATTTCTATAAGAAATAGACTTTTTTAGTGAAAACTTTAAATTTTGTTTTTCTTCTATTAATTTTTTATTATTTAAATTATCACTAATGTCGTAATATTTACCTTTGAAATTTTTTAAAACAAAATTTTCTTGATAAGAACGAATTTGAGAAGAGTTATTTTTTGGCTGAATTCTTAAGGAATCTAAGTCTAAAAAATATTTTTTATGGAAATCGCTATTGTTAAAATTTTCATTTTTATTCAAAGGATCAATGATGACACTTTCATTATTTATTAACGAAACAACCATATATCCGTAAGGATTAAAATTAACATAAATATATGGATTACCAAAATTGTCAAAAATAATTTCTATATCTTTTATTTGATAATCAATTTTCCTATTGTATTTAAATATTTCCAATTCCAAAAACTTTAAATAAGCTTTTACATCAGAAATATTATATTTTTTTAGTTCATTTATTTTTTTTGCATTATTTTGAACAAATTTATTGAAATTGTCAATTTTTTGCATATATTCAACTAATTCAAACATTTTATTCCCTCTTTGTATATTTAATTAACATATATTTTTATTATACTTTATATAATGAAACCCCTTTAAAAATGTTTCATAAAACCAGTAATCTAATAAAAAATAAGTAATTTTGGTTTTTTTATTTGAAATCTTTTTTTAAATATTTAATTTATTTTTAGTAATTTTTTTGGTTCTTGTAAAGATTATAAATTCATTATAAAAAGAATTTCTTGCATTAATTTTTTGAAACCTTCAACAAAAAAATGGCTGGTGAGAGCCATTTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGCTTTAAAAGAAACTTTTGCAACTTCGCGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGCGCCAAGTTTGAATTCAAAATTATATTTTTTGTTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGAAGCTGCTGCAACAGCCAGGGATTTTTCTTCGCCCTTGGTTTCAATAATTATTACATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAATTTTTTGAAATGCTTTTGTAACACTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGATTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATCAATTTATTATAACCGCTTTGACTAAGGCAAGTTTTTTGATAATAAACCAAATTTTCAATTTGATTAAAAATTTCAATTATTTTTTCAAGAAATAACCTCAAAAAATTTATTATCACTTTAAATTTAAAAAATTCAAGTGGATTTTTTTAACACTTTAATTTTTATATTTTTTTCAACATTACTTTTTTTCTTTGAATATATATTTATATTCAAAAATATAGTCATCTTCAAAACCTTCAATCTTTTGTTGATGTTCTTTAGATTTTGAATATTTTAAGCTGAAATCCATTTCAATTGTTTTAGGATTTGAATTCATATGAATTGTTAATTTGTGAATATTAATATATAAACCAGGCAATTCTTTTCAATCTTTTGCAGCACCTAATTCAAAGTTTTTAATTGTGATATCGCCACTTTTCAAATTTGCATAAATTTTAGTTTTTTTATAGTTCCAATATCCTGAATTATATTTAAAGAATGCTTCTTTTAAATCAACATTTTCATTATCAATAAATTTTGCTTCAAGTAAAACTTTTCTAGGAATCAAGAAAAAAATACATTTTTTTGAATTGGATTCTTTATCTTCTAGAAGCAAATCATCTATAATTCAATAGTCTTTTCAATATTCAAATCATTTTGAATCTGGCCCTATAGCAATTTCTAAAAAATCATTACGTCAAGGACCTGCTATACTTTTATTGCTTTGCAATACTGAATTATAAGATTTGGCACTGAATAAATTTTCTATATAAAAATGCAGATTACTATTTCCAATAATATTCAAAGCTTTTTTATGTTGTTTTTGTTCATTATAGCAAGATGTAACACATAACGGCAAAATTGGTAATGTTGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTATTTAAGACTCCATTTTAAAATATTTATTTTTATATTTTTTATAAAAAACATAAGGATCTTTATTTAGTATTCTCTTCAAAAATAAGTTTAAATTTTGAATATGGACCAGTTTTAAAATCATATTCGCTTTTTGGGTGTTGAATATATCACAAATGAACATCAAGTTCAAGTCTTTTTTTGTTTAGACTGCAATCTAACGTTAATTGTTTAATGTAAAAAAAGCGCAACTCGGGTTTACTATTCTTATCATCCAATTTATGCACCGGGGCCATATATTTATAAATTCATATTTTTTTATTTTTTAAATTTGTTGAAATTTTTCTAGGCTGTTCATTAAACGCAACATTAAGTTTTTCACTATAAAAGTCTGCTAAATTAAAATCTTCGCTTGCAATAAATCCTGTTTCTAATAATACCTTTTTAGGAATTAAAAACAAAATGGAATATGCATAAAAATCTATCTTTCCAAAAACACAATAATCTTTTTCATATTTATCAAAATTCTTTTTTGATGTTATTACAATTTTAAAAAAATCATTACGCCATGGGCCAGAAATAGTTGCGTCGTTATTTAAAACTGATTCTTCTTCGCTATTTAAAATATCAACCATATTAAAAACTATTTCTTTATTACCAATAATTGTTTCGGCATTTTTTAATTCAGTTTCTCTATTATTGCAAGAGGTTACAAATAAGGGCAATATTGGCAATGTAGAAATTCCAAAAATTGTTAATAATTTTCATTTATTTTTTTTCATTATTTAAAATCCTTTCCCGAATATTTAATTCCCTTTTTGATAAAATGTTTCCTTGTTTTATAAACCTTTCCATTTGGTTTCATGCCCAATAAAAATAATTCGCCAGAAAGACTTGAAGAAACAATTACTTGAGAATATCCTCCCCATCCATAATGGTAAAGAGGTTTATTTCCATTGTCATACATTCCATAAGCAACAATTGCATGACCAATTTTTCCTTTTTTTATTGGATTAGGCATATATGTTCCAAATATTAACCAAGGATAACCATCTCTAATTCATTTTCAAGGTTTTATTCACCCTGCAGATCTTCGATAAACATAAACAGGTCAAAAGTTTGGCTAATCATCTTTTTTTTGTTTACACCTTCATTATAAAAAAACAAAGAAAATAAGATATTTTTTTATTTAAAATAATGATGAAATTAGTTAAAAAAATTAATAGTCGGTTATTTTTTTATTTTTTAATATTAAATTTAGCGCAACCTAACTAGTTGATAAAAATGATCATCAATTTATTTATAAATAATTGTTTAGTTGAATTTATATGCCTCTATTTTAAGTTCTACAATTTTTTTAAAGTTATATTTAATGTGACAAAAAACCTATGCAAAAGTTATAATTTCGCTTCTTGCATAGGTTTAGGCTTATTCTAATTTATTATCTATTTTTTCTAATGCTTTTATTGTTGTTTTAAAAGAAACTTTTGCAACCTCGCGCAATGTCTTTTGACCATATTTCAAAATAAAATTTGCTGCCAAATCAATAATTTTATTGCTTGCACCAAGTTTGAATTCAAAATTATATTTTTTGTTTTGTTGTTGCATTCAATCTATTAAAAATTTTCTTGATATTATTGATGCTGCAGCAACAGCCAGGGATTTTTCTTCGCCCTTGGTTTCAATAATTATTTCATTTTGAGGTTTTTTAAAATCTAGTCAAGAAATTGCTTGCAATTTTTTGAAATGCTTTTGTAACACTTCTAATGAAGTTGTATATTGATCAATAAAAACATCAATGTTCTGATTTAATTTTTTTGTTAATATATTTATTGAATTACTATGAATTAATGTTTTTATTTCGTTGTTATTAATACCTGCATCAATTAATTTATTATAACCACTTTGACTAAGGCAAGTTTTTTGATAATAAACTAAATTTTCAATTTGATTAAAAATTTCAATTATTCTTTTATCAGAAAGTTTTTTGCTGTCTTTAACACCAATTTGTTTTAGTTTTTCAATGTTATCTTTTGGTATATAACAGGCAACTGAAATTACGGGACTGAAATAATCACCTACTCCCGTTTCATCAACGCCAATTAATTCATCTAAATTTTTATTCAATTTCTTCTTCTTTACTAAAAAAGTTATCTGCACCTACAGTTTTAATATTATTTTTTTGTTCGGCCTTTTGTTTTTTACCTAATTTAATGTATTTTTCTGGGTTTTTTAGGAATTGATCAATAAAGTATCCAACTGCGCCTTCTTTATTTGTTTGTTTCATAACAACTGTTGACATCTTTTTAATTAAGGGTTCAGCATTCGCAGGTGATACACAAACATTAGCAACATTATACATGCTTAAATCATTATATGAATCTCCCATTGCAACAGTATCATCAATGTCAATGTTGTAATATCTCATAATTAGTGAAATAACTTTTCCTTTATCAATACCTATTGAAGTAATATCAAATACTGGGCTTAATCCTTCGCCTTTTGATCAAGAAGAAAATTCTCCTAAATCTCCATAACGTCTTTTTAAATATGTCAATAATTCCAAAACATCTGTATCAGGTTTAACATCAAATACAATTCCGGTTGGTTTTAGTGGAATTTTTTCTAGGTTAATACATTCTCTAAATTTTGTTGCTTGGTTAAATCCAAACACTCTTTCAAGGTTAGGATCTCTATGCATTAATTGAACCCAGTCAGGACCTTCTATTGCATAATTAGTAATTTCTTTTTTTACTTTTTCATCGCCTAAAATATAAAGAACTTCATTTAAGTCCAAATATGTAATAGCAGGTATAAAGAATGGGTCAGCAGGGTTGTGGATGTGAGCACCATTGTAATTACCTACAATTGCATTAAGTCCAAGTTTTTCATACACAGGCAATGTACTTCTTCAAGGTCTTCCTGTAATTATTGAAACAATGTGTCCTTGGGCAACAGCTTTTTTAATTGCTTCTTCTGTTTTTGGATGAACTGTGCCATTGGCTGAATCTGCTAATAGTGTTCCATCTAGATCAATAGCAAATAAAAATCTTCTTTGTTTTGATTCTTTGTTGTATGCCATAATTTCCTTTCGTAATATATAAATATTTTACAATATTTTTTATATCTTCTACATTTTGAACAAAATTACTAAAAAATGTATCTCAATTTTCAATGTTTTATTGCAAAAATTTACACGGAATTAAATTTATTGCAAAATAAAAAACTTCAAGTTTTTTTACTTGGAGTTTTTTAAAAATTTATTTATTTTATTTTTGTTCCAAATATACGGTCACCAGCATCTCCCAAACCAGGTAATATATAATTATGTTCATTCAATTTTTCATCTTTTGAAGCTAAATATATATCAACATTAGGAAATTGTTTTGTTACTTTATCAATACCTTGTTGAACGCCTACTAAACATACTAATTTAATATTGCTAAAGCCCAACTTATCCAATTTTGCTATTGCATCGCATGCAGATGAGCCGGTTGCAAGCATAGGGTCAACAATAATAATATAGCTATCATGTGGTACTTCTGGAATTTTAAAGAAATATTCAACAACTTCGTTTGTTTCTTCGTTTCTATACATTCCAATATGACCAACTCTAGCTTGGGGAACCAAATCCATAATACCATTCAACATTCCAAGCCCCGCACGTAAAATAGGAATGATTACAATTTCTCTATCAAGAGTTTCGCCTTCATATTTAACATTCATTGGTGTTGTTATTGATATTTTCTTAGTTTGATAGTCCCTCAATGTTTCATATACCATCAACGAAGCAATCTCGTTCAAATTATCTCTAAAATCTTTGTAAGATGTTTCTTTTGAACGCATCGTTGTTAATTTAGCGTTAATTAAGGGATGCTGAAAAATTTTTACCATATATTTGTGTTATCAACTTTCTTTTTATTATTTTTCTAATTTAAATAATTCTTCCATAACTGGAACTGATTTAGACATACCCAAACGGTTTGCGCCTAATCTAATCATTTCTTGGGCATCTGCTAGATTTTTAATACCACCTGATGCTTTAATTAATAGCTTGTCACCACAAGTTTCTTTCATAGTTTGAATGTCTTCAAATGAAGCTCCTCTATAAGAAAATCCGGTTGATGTTTTAATAAAATCGGCACCAGATTGCATTACTAATTCAGTAATTTTTATTAATTCAGCTTTTGTTAACAATGCTGTTTCAACTATAACTTTTAAAGTTTTGCCATTGCATGCTTCTTTTATTGCTTTGATTTCATTCAATACGTATTCATATTGTCCATCTTTAAATCTTCCAACATTGATTACCATATCAATTTCGTCAGCGCCATGTTCAACAGCAATTTTTGTTTCATATACTTTAACACTTGTTGCATTATAGCCTAGTGGAAAACCAATAACTGTGCAAACAAGCACATCACTGTTTTTTAATGCTTCTTTAGCATACTTAACATATGATGGTGCAATACATACTGATTTAAAATCGTATTTAATTGCTTCTTGAATTAATTTATCAATGTCTTTGCTTGTTGCAGAAGGTGATAAATTTGTGTGGTCAATATATTTGTTTAATTCTGTCATATTCTTCCTTAAGTTAATTTAATTAATTACATTAATTTAGCAAAAACCGTTTGAATGTCATGCTTGCTTTCGCTAAATTCTATTGCAGACAACAATTCTTTTTGAAGTTCTGGATTTATAGGCTTTGATGAATACAATGTAAATAATACATCTCCTTTATTTACATATTCATTTGAACTCTTATTCAAATAAATTCCTGCTTCGTAGTCGATAGAATCTTCTTTTTTAGATCTACCTGCTCCCAATTTCATTGCTACTAATCCAAAATCAATTGCAGATTTTATTTTTAAATATCCACTTTGATTTGCAATAATTTCTTGTTTATATTGAGGATTAAATCATTTTGAATCTTTTTCAAAAACTTTGATGTTTCCACCTTGTGCTGAAATTCATTCCAAAAATTTGTTATATGCTTTTTTATTATTGATTACTTCATCAATCATTTTTCTTGCTTCAACTTCATCTTTAGCTTTTTGTGCTAAAACTAATAATGTTGAACCTGATGAGTAAATAATTTCTGTAAAATCTTTAGGTCCGTGTCCATTTAATGTATCTATTGCTTCTAAAACTTCAATTTTATTGCCAATAGTTTTTCCTAAAGGTTGTTGCATATTGGTTATTTCAACGGCAATCTTTCTATTTAATTTCTTGCCGATTTCAATCATCAATTTTCCAAGTTTTTTTGCTTCATTAATATCTTTCATAAATGCGCCATTGCCACATTTAACATCCAATAAAATGCAGTTTGAACCAGTTGCAAGTTTTTTAGACATAATTGATGAGGCGATCAATGGAATTGATTGAACGGTACCCGTTACATCACGTAATGCATAGATTTTTTTATCTGCAGGCACAAGTTTTTGATTTTGTCCAACTATTGCAATACCATGCTTTTCAACTAAATCTCTAAATTGTTGGTCAGTCACTGAAAAATTATAACCGTCAATTGATTCTAATTTATCAAGTGTTCCTCCTGTATGTCCAAGCCCTCTTCCTGACATCTTTGCAACAGTTAAACCTAAGGCTGCTAAAATTGGGCATAATGCAATACTTACTTTATCTCCAACTCCTCCTGTTGAGTGTTTATCAACGATTACTTTATTTAAATAACTTCAATCAAGTACTTCGCTTGAGTGCATCATAGCATCGGTTAATTGCGCTGTTTCAGCATCGCTTAAACCATTTAATCTGATTGCCATTAATAAAGCAGCAGCTTGGTAGTCTGGAACATTTTCTGCTACATATTCTTTAATGAAAAAATCAATTTCTTCTTTGGTAAGAGTTTGATTTTCTACTTTTTTGTTAATAATATCAATAATTCTCATAATTATTTCTTTCTATTTATTCAAAATCTTCTGATATTGATAATGCTAATTCCATCATTTGATTGAATGAATTTTGTCTTTCTTCAGCGGTTGTATATTCATGTGTTATTAAATTATCAGAAATAGTTAAAATTGTTGCAGCTTTTTTGTGCAATTTTTCTGCTACTGTAAATAGTCCATATGCTTCCATTTCAACAGCAACTGCTCCGCCTGAATTTTTAACTCTATCTTCAACAGAAAATACTGAATAAAAGGCATCTTCCGATAAAATTCTTCCTTCTTCAAGTTTTAAATTCAATTTCTTAGCATGTTTTCTAATTGAATCATTTAATTCACTATTTGGAAGAGCTACATTACTTTCATCATTGACTAATAAATTTTTACGGAATGAAGTGCTATCACTATATGCTGAAGTTGCCAAAACTAATGAATAATTTTTTAAATCAGCCTTGAATGAGCCACCTGATCCAATTCTAATTATTGCTTCAACTCCATATTGGCTAAATAATTCATATGAATAAATTCCAATTGAAGGAACGCCCATGCCTGAAGCACAAATAGAAATTTTTCTTCCTTTATAAAAGCCAGTATACATTAAAACATTTCTAACTGTTGACACTAACTTAGCGTTTTCTAAATAGTGTTCAGCAATATACTTTGCTCTTAAAGGATCACCTGGCATTAAAACTAATTTTGCAAAATCATCATCTTTTGCATTTATATGAGGTGTCATATTTTCTCCTTATTCTATTGTTAGTTTTTTATACATTATTATTTTATAATAATTAAATAATTAATAAACATTAAATGTCTAAAACGCTAATAAGTCAATACATAAAAAAGCAAAAAGATTAACTTCTTGCTTTTTTATGACACTATAAACCGCTTTCTGTACTATTTTAATAGCGCCAACTATTTATCTAAGCCAATCGGCTTCCGGGTTCTTTGTTCGATTCCTTGTTCCCAGTTCCCCTACCAAAATTTGGGTTTCTAGCTCATGGAGTTTACATCGTTGCATCTTAACTAAATAAGCTCGTCTCTGTTGCACTAGTCGTCAACTAAATTGTTGCCTAGACTTATTGGGTCTAGCATGAACACTACTCGCATCACAGCGAGTGCTAGAGCGGATTTTCCTCTACTTTGTAGTAGCAGTTGGCCATGTAATTTAATTTTACCTTAAAACACAAAAAATATCAATGTTTTTTTAGTAAAAAAATATAATTTTAATATATGGAATTTGAGCTAAGTGGAATTGTAATTAATAAACAAAATGTTAATGAATTTGATGAAATAATATCATTACTAACAAATAAAGGCATTCAAAGAATTTTTTGCCCCGGGGTAAATAAAACTAATTCAAAAAATGGCCCATCAATTTTAATTTTAAACTTAGTTAATTGTGAAGTCATTAAATCAGAAACAAGAAATTTATTACCTAGACTAAAAACTGCTTCGTTAATAAAAAATTTTCCCACAGATTATAGACTTTTAAATATTGCAAATGATTTAAAAACCTTCTTTAATTGTCTTGATAATTGCAATAATATTAATCTTATTAAAGAATATGATCATTGCTTAGATTTTATATTGTCAAAGTCTGATAAAGTTGAAGCGTATTTATTGTTTTTAGTTGTTTCTAGTTTAGGATTTAAGCCTAACTTAAATTCTTGTATTGAATGCAATAATGTTGAAAATATTGTAGATTTTAAACTATACAAAGGGGGATTTTTGTGTTCGATGCATTCAAACATTGTAAATGCTGATGTATCGCTATTAAGGTCTTGATATTGAATAAATAAAAAATTTGAACAATTTGATTTAAATTGTTCAAATCTAAATGCTATTAAAATCAATAATGAACTTAAAATGTTTTTGTATGATAATAACATTATTTATCGTTAATAGATATTTTTTTTATCTCATCAAACTTACGTTGCAATGTTTTAAAATCAATGTTATTTGTTTTATTAGAATATAACATGTCTTCAAAATCATTTAATTTTATTAAATCTTCCTTTGATATATTCTTTTTATGTAAATTAATAATTAAATTAAATGCTTCATTATAAAATAGTAAGTATTGTTTAAAATTATCTTCTAAATATTCATATAGATTTTTAAAATCATCAGAAATTTTAGTCAATGTTTCCTTGTCTTGTTTATCTTCAATATATTGACTAAAAATATGTTCATTCTTATCCACTATTTTTTTCTTAAAATCATATTGAGCAACTTTTTTTTCAAAATAATCTCTTAAGTTTCAAAATATACCATAATTAGGCAATGAATGAATTAAATTTAATGAACGTAAATATTGCTTATAAATTTCTTTACGAATTATTATTAATTGACTGTTTGAATTAGTTTTTTTGTGATTATTAACAAGAACATAAGTAATACCTAGCAAAAAAGGCAATCCAATAACTGCACTAATTCCTGAAATGATGCCTATTTTTGATCTTCTAGTTAAAAATCTATGTGGTTTTTTGTTTTGAACCTTTTCTTGATTCTTTGATAACTTCATAACAAAATTATATAGTTTAAATTAGTATTGTTATTTTAAAAATAGTAGAAAAATTTATTATTTTTTAGTAGATTTTGCCAATAGGATAACATTTTCATCAAGGTTTGGATCTTCTTCAATACTTGCAGTAGTATGATTTGTAAACAAAATTGTAGGAATAATAACAACATCTCTACGTTTGTTTTTATAAAAGAATGGGGCTAATCTATCAATTATTAGTTGCTTTAATTGAGGTACAGTTCAATCATTTGAGTTTTTAATTGTATATAATATAGCGCCGTGAACCAATCTCTTAGCTTCTTCAATTAAATGCAATGAATTTTTTACATAAAATGCGCCACGTGAAACTATTCTTGGTTTACCAATAATTAGATTCTTTTCTTTATTAATTGCTAAAACAACATGCATAAATCCGTTTTGGCCTAATTCACTACGTTCTTTAATAATTTGAGAGTTAGTTTTTGATAAAATATTGCCATCAATAAAAACAGGCCCGAAATAAACCTTTTCGTTCGACAAACGAACTTGATGATTTTCTAAATAATAAACATCCCCTAATCTAGCAATCAAAATGTTTTTAGCATCAACACCGCTTTCAATAGCAGTATAGCCATGAACAACGCTCATTCTATATTCACCGTGATAAGGCATAAAATATTTTGGTCTAGTCAATTCAAAAATTTTTCTATGTTCATCTTTGTAGGCGTGGCCCGAGGTATGTAAATAACCGTCTACTCCATTTTCACGAATTATTGCGCCTAATTTATATAGTCTGTTAACTAATAATTCAATTTTAGATCTATTTCCAGGAATTGGACTAGATGAAAAAATAATAGTGTCTTTTGGTTTTATTGTAATATTTGGATGTTTTCCTCATGACATCTTTGCAAGTCCAGCTAGTTCTTCACCTTGAGAACCAGTTGTTAAAATTAATAACTCATTATCTCCAAATTTGCTTAATGATTTTTTGTCAATAAACACTTCATCTGGAACATTAATATAACCTAGTCTTCTACCAATATCTATTCCATCAACCATAGAACGGCCGAAGGCAACAACCTTTTTATTTAAAGAAGCGCCTAATTCAATAATTGCTTTAATTCTAGTTAAATTAGAAGCAAATGCAGTTACAATGATTTTCTTTTCTGCTTCCAACATATATTTTTTAATATCTTTTAAAATATCTGACTCTGATGGTGAATGATTTGGACGCATAGCATTTGTTGAATCAGAAAATAAAACATCAAGACCTTCATCGCCAATTTGTTTAAGTTTATTAAAATCTGTAAAATTTCCAATAGGTGTATAGTCAAACCTAAAATCGCCTGTCATCATTAATGAACCGTTAGGAGTTTTAATTCTAATGCCAAAAGCATCAGGAATTGAATGTTGGGCAGTTCAAAAATCTACTTGACAAGGGCCAAAAGTTCAAACTGCATCTTTTTCAGTTTCAATAAATTCAATATTACGTTTTATCTTCATATCCTCAAATTTTAGTTTTAGATATTGAATAGCAATTCTAGGTGCAAATATTTTTTTAACATCTACTTGTTGAACTAAATATGGAACACCTCCAATATGATCTTCATGTCCGTGGGTTATGAAAAGACCTTCGATTTTATCTTGGTTTTCTGCTAAAAAAGTGAAATCTGGAATAGAACCATTTACACCTGTTACATATGAATCGCAAAATTTAATTCCGGTGTCAATAATAAAAATATGATTTTCATATTGAATAACCAAAGTACTTTTACCAATTTCTTGCATTCCGCCTAATCCAAAAATTTTAGTTGGGCTCATAAATCCTCCAATTTTTATAATTATTATAATGAATAAATATTCAGCTTTTTATTGTTAATATTTTAGCAAAAAAATACGAAATTAAAAAAAATACAAATTTTTTATTAAAATTTTAGATATGAATGATAGTAGGGTACAAAAAACAAAGATTCCTAAAGTATTAGCAAGTTTTAGTTTAATTTTAGAACTTATTGCATTAGCATTACTTATTTATGTTATATATAGTTTCTCTTCAATTTGAAAAGAAACACTTGCAGATATAAAGGGAATTATAAAAAATAATAAAGACAGTTTTGAAACATTTTGAAAGGAATTTTCTAAATATTGAATAAACTTTTATAAAAATAATAAAGTTTTAATTAGTGCAATTTTAGCATTGCCAATATTATTATATGTTTGCTTTATATTTAGCATGTTAGTAATAGCAAAAGCTAGTTGGTCATTACACAAAGTTCCAATAATACTTATTATTGTTACATTATGTTTGCCAATTCTATTATTATGTGAAATAGTTAGAATAATATCACTATTTTGAATAATAATCAAAAAGCAAAAAAATTTCAATAACCAAGTGTACTATGGACCACAAGTTGCTAACATACAAAATTAACAAAAAATTCGTAATTATTTCTATTACGAATTTTTATTTATAAAATAAGAAAACAAAAAGAAATCTTATTTATATAATTTATCGATTTTATTACTTATTGCTCTTGCAGCAATTGTCCCATCAGCTGTTGCTGTTGTAATTTGTCTTACATCTTTTACAATAACATCGCCTATTGCAAAAATATTAGGAATTTTAGTTTCCATATTTTCATTAACTTCAATAAATCCACGGCTATTTAATATTTCATCGTATTTGCCTTTTATAAACGCTGTGTTAGGTTTAAAACCAACATAAGGAAAAATGCAACTTATATTCATTTTTGTTTCTTTTCCATTTACATTAGCAATAATTTCTTCAACTTTTTCATTGCCCAAAATTTCTTTAATAGTTGAATTTTCATGAATATAAATATTGCTTTTAGACATTGCTTCATTGACAAGTTTTTTTTCTGCAATAATTCCATCACGAACAAAAATATGAACCTCAGAGGCAATTGATGCTAAATAAGACGATTCTTCGAATGCTGAATTACCTCCACCAATGATTGCACAGGGTTTATTTTTATATAAAGGACCGTCGCAAATTACGCAATATGATACACCTTTGCCTCTATATTCTTCAATACCTTTTACATCCAAAGGAACAATTTCTTTCATGCCAGTTGCAATAATTATTGCTTTTGATTTTATTGACATGCCATTTTCCAAAATGACTTCTTTTTCAATATCTGATATAGAATTAATTTCCTTTACATTACCGTAAATAAATTTTGAGCCATTATCTCTTGCTTGTTTAAGCATAGCTTTAGAAAGTTCAAAACCACTTATAAAACTAAAACCTGGATAATTTTCAATTTTAGATTGGGCAGAAACTTTTCCTCCCGGTACTCCGCCTTCAATAAAAGCAACATTTATGTCATTTCTTGCTAAATATATTGCAGCTGTTAATCCTGCCGGCCCTGCCCCAATTATTAAAACATCATATAAGTTATTATTTTCCATATATTTCTCCATTACTAAAAAAGCAGCAAATTATTAAGCTGCTGTTTTAGTTTCTATTTGATTTTTAGTAGTCGATTTTTTAAATGTAAATAAATTTGAATTTTTAGGTTCATAAACTATAAAGTAAAAGAATTTTGAAAGCTTTTCGTTATTATATGTATTAACTTTGTAAACATTGTAATTCAATTTTATAACGAATTCAAACATTACAGTCAATGCCAAACCAACTATTATATATAAAGCAGAAATTACTTTATAAATAGTATAACTGCCATCACGTAAAGGTTCCATTCCAAAACGAATTATTCCATAATAAACAATATATAAACCACCAGTTGTTCCTGGACGTAATCAATTTCAATAATTCAATATTCAAACTAAGATAATATAAGCAGCTAAGTTTGCAATTGATTCATAGAAAAATAAAGGATAACGATATTTTCCATCAATAAACATATGATCTCTAATTAATTTTGGCAATATTTCAAATGCGACAGAACCTTTTTCCATAATGCCACCATAAACTTCATGGTTTGCGAAGTTTCCCCAACGTCCAATTGCTTGTCCAATTAATACTGCTGGTAAAATTATAGTAATTGCTTTTTTTAAATCAATTCTATTTTTATTTTTTGAAAATAGAATATAAAGAATACAACAAATTGTAGCAGTTATGACACCACCATGTATTGATAGTCCACCCTTTCAAATAAAGAATGCATTATACCAAGGACCATCTGCCCAACCTTTATGTTCAATTAGTTGTTGAATAACGAATACAAATCTAGCTCCGATAATAGCAGTAGGAAGTGCTATAAATACCAAAATTGTTAGTTGTTCGAAAGTATATTTTTCTCTTCTTCAGAAAAAATAAACAGTCAATATAGCAGTGAGCATTCCTAACATCATTGTTAAGGAATAAACATTCATTGGGCCTAATTTTAATTGAATTCCCGCTCAATCTTTAACTTGCATTTTCCCTCCTTATAAATTTGCCACTTACATTAAAATATAATTATAAATTATACAAATCAAAAAAATTTTTTTTAATAATCACTATAAAAGTTTTACTAAATATTGCCCCGTGTACGAATTTTCACATTTTGAGACCTGTTCAGGTGTTCCTGAGCAAATAACTTTTCCTCCTTCGTCCCCACCATCAGGGCCTAAATCAATAATATGATCAGCACATTTAATTAAATCCAAATTATGTTCAATTACAATTACTGTATCGCCATTGTCCACTATTCTATTTAATACTATTAATAAATTAGAGATATCGTAAGGATGTAATCCAGTTGTTGGTTCATCTAATACATATAAAGATTTGCCTGTTGGCTTTTTTTGTAGGTATGTTGCTAATTTTACCCTTTGAGCTTCACCACCGCTTAATGTCGTAGCATTTTGGCCTAATTTAATATAGCCAAGTCCAACATCTAATAAAACTTGCAATTTTTCTTTAATTTGGCTCTTGTTTATAAATAATGCATAGGCTTTTTCAATTGTCATATCCAAAACATCGCCAATACTTTTACCATGATATTTTATTTCTAGCGTTTCTTGATTATAACGTTTCCCGTTGCAATGGTCGCATGTCACATAAACATCGGGCAAAAAATGCATAGCAATTTTGATTACGCCATCGCCTTCGCATTTATCACAACGCCCACCTGGAACATTAAATGAAAATCTTGATTTTGTATATCCTCTAATTTTACTTTCTTCGGTATTTGCAAAAATTTCTCTAATATCGTCAAAAACACCGGTATATGTTGCAGGATTTGATCTAGGAGTACGCCCGATTGGATTTTGGTCAATTTGAATTAATTTATCAATGTTAGTTTGGCCAATTAGTTTATTATCTCTTTGAATATCATAATATTGATCTAATAAATATTTATTAAGACTTCCAACTAGTTCATCGTTTATTAATGTAGATTTTCCAGAACCACTAACTCCAGTAATTGCAATAAATTTTCCTAACGGAAAAGTTACTTTAATATTTTTTAAATTATTTAATTTTGCATTTTTTAATGTTAATGTCTTGCCATTTCCACTCCGCCTAAATTTTGGCACCTCTATTTGTCTTTCTTTTGACAAATATTGGCCAGTTAGACTTTGCTTACATGCAATTACATTTTCAACATTTCCACACGCAACAATATTTCCACCATGTTCGCCTGCTTGAACACCAATATCAACTATATAATCTGCTTGTCTAATTGTTTCTTCATCATGTTCAACAACAATTAAACTATTACCTAATTCAACCATTTTTCTTAAAGAGTTTAGCAATTTTTCATTATCTTTTTGATGTAAGCCAATTGATGGTTCATCTAAAACATATAAAATGCCTGTTAGATTCGAGCCAATTTGTGTTGCTAATCTAATTCTTTGAGCTTCACCACCGCTTAATGTCTCAGCTTTTCTATTCAATGTTAAATACTTTAAGCCAACATCAATTAAAAATTTAATTCTATGTTTTAATTCAGACAAAATTAAAGAGGTAATTTCTTTTTCTAAATCACTTAACTGCAATCTTTCTAAAATAATTGCTAAATCCCCAAGCGACTTATTGCAAATTTCATATATATCTAGATCTAGAATTTTGCAAGACAGCGCATACTTATTTAAACGCTTTCCATGGCATGAAGGACAAGTAATATCTCCAATGTATTTACTATAATAAGCCCTAGCTTCTAGTGATTTTGTATCAAAATACTTCCTTTGTAATTTATCAACAATACCTTCAATAACTTGAAACTTCTCGCGTTTTGTACCACTTTCAGATAATAACACATATGATATTTCTTCTTGCGACCCATGTTGAATAATATCTAATTCAAAAGAAGATAGAGAATCAATGGGTTGGTCTTTATCAATATGATAATAATTTAATAGCTTGTCAAATTCTTGCCATTCTAAATTGTAAGTATTCATGAAATTTCTATAATACTTTATTGCTCCTTCGTTTATAGAAAGCTGCCTATCGGGACAAATTAAATCAAAGCTTGCTTTTTGAATAACACCAAGTCCCTTGCATTCAGGACACATTCCGGCTGGAGAATTAAACGAGAATAATTTGGGTTCAATTTTTGGCATATCAAAGTCGCCAAAACTGCAACTATGATGTATTGAATAGGTATTCTTTTTGCTTTCATTAACAATAGTTGAAATTAAACCATTAGATTGCTCTAATGCAACCTCAATTGCACTTGATATTCTTGATTTTATATCATCATCATTTGATAATTTAACTCTATCAATAAATAGATCAATATTTCAACGTTTATTTTTATCTAGATTAATTGAATCAACATTTTCTAAGAAGTAAATCTCATCATTTATTAAAACTTTTATAAAACCATCTCTTTTTAATTTATTAAGTAATACTTGGTGGCTTCCTTTTTGATTATTTACTAATGGGGCTAATATGTATATTGTTGATCCTAATGGGTTTTTATAAATGTTAGAAATAATGTCTTTGATTTTTTGAGCAGTAATTTCAATTTTGTGATTTGGACAATAAGGTTTTGCTACTCTTGCATATAGCAATCTTAAATAATCATAAATTTCAGTAACAGTACCAACTATACTTCTGGGATTAACATGTACTGTTTTTTGTTCAATAGAAATTGCTGGGCTCAATCCTTCAATGCTATCAACATTAGGTTTTTTAGTACCACCTAAAAATTGTTTTGCATAATTAGAAAGGCTATCAACATACCTACGTCTGCCTTCTTCATAGATTGTATTAAATGCTAAAGAACTTTTACCAGAGCCACTGACACCAGTAAAAACCACAAATTTGTTACGTGGAATATCTAAATTAATATTTTTTAAATTATTTTCTCTAGCACCTTTTATTATTATTTTATCGTTCATTTTCTTTGATATAACCCTTTTCTATTAAATATTGTTCTGCTTTCTTTATTCTTTTTTTAAAAAATTTATTAATGAATTTTAAGTATTTCATATCCATTTTAATTTTATTAGGTTGCCTAAATTTAAATTTTTTAAGTTGTTTGATTCAATCTAAATGTCTTTTTCTAATAAAAATTTTTAGTTGTTGATTAAAACTTTCAAAAAACCTAGTCTTGCGATTCTCATAGTCATTTAAAAATGCAGTTTTTGCAATTGATTTTAAATAAATAGGATAATCAAATATTAAACTATTGTTGAAATCAAATATTGGAGCTGGCTTTGTTAAAATTCCACTATTATTATCCAACATCATGCCAAAATTATTTAAATGTCTATCCCCATTGCAAATTAAAGCATCAAAAACCATTAAATCTTCAAATAACTCTTTGCCATAATAAAAAGCAATCTGACTACATAGTTTTAAATTATTATCAATACTATTGGGATTTATGATTTTAGAAAATGGGATAAATGAAAAATCTTTGTTTGTAAACAATTTGCAATCACTAACAAAATGCTTTCCATACTTAGTAATTTTATAATCAACCGCCGATAAATTCAAAAATTTGGCAACTTGACTTGCAAAATATTCACTATAAACATCGTATAAGTTTTTATTTGCAAATTTTGAGTTTCGTTTATATAAATGAATTTCGCCATCCACCTCAACTCATGCTTTTAACATGTCGCCATCAATAAATCAACTAGGGCTTTTAATTTGTAGTCTGTTAGTTTCTGTTAATTTTGCTTTATAATCTAAAAAATAATTGCAAGAATCATTATTGAAATGATCAAAATAATTTACATCTTCTCAATTTAATTTGCAACTTTCTTCTTGGCTACATATCCAAAATGAATTGCACAAATTCAGATAATTATTATTTAAAATTAATTTCTTATAATTTAAAAAGTTTTCAATTAATCCTTTGTAATTAGGCCTTTGCATTGGAATTAATGAAATATTTAAAATGAATTCAAGTCATTGTCTTGGTGAATATTCATTATATTTTTGCTTTATAAAAATAAAATCATCAAGCAATTTAACATTGCTCAATGTTGTTAATTTTTCATCTGCTTCAAAACTTAAACAAATCTTGTTTTTACATTTTAAATAATATGTTTTCATTACTAACTAATTTATTTCGCCATCAAAATCGTTCTTATTATCTTTTGATTTTGTTTTTGGATCTTCTTTGTGGTCTTCCTTTGCTTGATTTTTATACTTTTCTTCAACTTCTGCAATTTCTTTTGAAAGATCACGATGCAATATTTTTTGTAAATTTTCTAGCATGCTTTTTTCTCATGAACCATCATTAGGAATATTATTTATTCCCATTCCCCTTAGTTCATCGAATGCGCCCTTGAAATAATCAACCATTTCGTCATCACGAACATTTCCAATTGCTTTGGCAGCTTTATACATACTATCAACCAATTTTTCCATACTTAGATCTTCATAATTCATTTCAAATGGAGAACCAGACATAAATTTATTATCAGCAATTTGTTCTTTTAATTTTTTTGCTTGTTGCTTTAAATCATCAAAACCTTCTAAGAAATTTACAAATGAATTTTTAAAATCATCCATACTGCTCATATCTATTTCTTCATCATTTGAATCGCCATCATCTGATTTTTCATCAGATTCTTCGTCTTCTGATAATTCAGATTCAAGAGAAAATTCCTTAGTTCCAAAACCACCATCATAATCTTCTATTGACATTCTACGTTCAATAGGGAATGCTTCTTCTATTGCTCAAATTTTGTTTCCCAATAAATTTTCTACTGAGTTTCATGCATCAATACCATTTTCTATTTTTGTTAATTTAAAATCAAAACTAGATGGTTCGGTTTGCAATAAATTAAGTTTTTCTGATAATTCAAATAATTCGTCTCATTTGCTAAAATCACTTCTAGTTATATTTTTAATATCATTTGAAGGGTTTGTGTGAAATTTTTTAAATTCATTTTGCTTTAATTCTTTTAAATATTTTAAAGTTTCTTTTCTTTCAGAAATTACACTTTGACTAATTAATTCAGGATCAATTTGAAAATCTTCATCAAATTCTCTTTTATCATCATAAACAAAATCAATTTTTAATGCTAATGAAATAATTTCTTTTGTAGGTAAAATTGCAGAGAAAATAATTTCATTTTGTTCAAGTCCATATTTTTTAAAGTCAAAAGAATTTTTATTTTCTTCATCGTCGTTTATTAAAAAATCATTAAAAATATTTAAGGAATTAAAAGTGTTTAAATCTGACAAAAATTCTTGTGATGCTTGTAAATCGCCAAATACAAATTCAAATGGGACAATTGTTTTTAAATAATTTGGAATTGTATATCAATGAAATACCACGCTCTTTTCAAAACCAAAAAATGCGCCTAAAACATCCACTAATTCAGAAAAAGAAATCCCCGAAGTTATTGGAAATGATATTGGTTCAAAATTAATTTTTTTAACTGCTTCTATTGGTTTAAATTCGTAGTCAAAAGCTACATTTAATTCTCTAACTGTTAATTTTAGATTTATTCCTAATTGTTTCTTAATCATTTTCTGCTCCTCTATTGTTTTCAAATTCATGCAACATATATTTTTTGGTTATATTTTTTAATCTATATATGTAATCAAGTATTGCTTCTCCCAATCCATTTTGTTCAACAGCACTTGTATGAAAGTGGCTATGTCCATGTACAGTTTGTGTTGCATTTGCCATTGCATAAGAATATTTTGATATTGACAACATTGGTATATCATTATTTCCATCCCCGATTGTCATTATTTCATCTTTAGATGTTTCTACATTCTTGTAAACATTTTCCAACATTCAAAGAACAGCAGACCCTTTTGAAATATTTAATGGTGATATTTCAATATTACAATTTGTTGGGATCATTGCCAAATTTGTTATTGACTTAAATTTTTCAAACATTTGCAATGCTGATTCTTCCGTTTCAGTTCCATCTGCTGAATAAATTTCAATCTTAGTTGGTTCAATAAATTCACCTTTATATAATTTAGGAATCGAGTGTAATAATTCCTTGCTTATAAAGTGATATGAGCAAATATATTCAACTGATTTTTGATTATCTTTTGTGTAATAGTAATTATTATTGTCTCATAAGAGAACTTGGAAATCATTTTTTTTAGCAATGTCAAGAATCAATTTTGTTGTATCAAAATCTATTTTTCAACAGGCAATATATTTTTTTGATTGCATATCAAAAATTTGAGCGCCGGAAGATCCTATTAAATATCTTGCTTTTAATGTTTCGGCTAGTTTTAACATTCTTTCTTGAATTGGATTTCCGGTGCATATATTAAAATCTAATCCATTGTCTACTGCAAAATGGATATCATCAATAGTTTCTTGCTTTAAATTAAAATTTTTATATATAGTACCGTCAACATCTGAAAAAGCAATTGGCTTCTTGTTGTTAGTAACTTTTAAAATTGTTTGACTCATAATTCTCCTTGTTTTTTTATGCTCTTGCCTTTGATTTATGCTTCTTTTTTAATACCATTATAATAATATAAATTATAATGCCAGAAATGGCAACTGGTGGCACAATATAGCTTAATAGTCTAAATGTATAATGCATTGTAGTTTCGGGTTCTTCTGATTTTAGAAATTCCGTATAATCTAATAAAAAATGATTTATGGCATATGTTTTTAAATTGAAATAATTCTTTACATTTAAATCCGAGGCATCTTTTTTTATTTTATTACTTAATTGTTGTTGCATTTTTTTCGATACTAATTCAAACACAAAAAGTATTTTTAAAAGTTCATCATTTGTTCTTAATCTTATTTGAAAATATTTATTAAAAACCCTATAAAGATTAGGATCGGGATCATTTTTTAATAAATTAATAAAACTATTTTGATTCTTTATATCAAAGAAGGGATATTTAAGATCATTATCCAACTCCAAAGCTCTATTGTATTCAGACTTTTCTTTAAAATTCCAATCTTTTACTCTATTGTTATAAAAATCTTCAAATTTTATTTTTTTAAATGGTTTTTTAGGGTCTTCTATATTTAAATTTTGTAAGAATAATTTTTGATTGATTTCATCAAATGAAAATCTGTGTTCTTTAAATATTTCAACTATTCTTTTGGCAACACTTAAATCTTGTTTTTCAATTCCGTCATCATTGCCTAATTCTAAATTATCTTGATTATCTTTTGATTTTTTGCATGATAATGAAACTAATGGGATAGTTAGTAAAGTCGGAATAGCCAATGAAAAACTTAAAAATTTTTTTATTTGTTTTTTATTCATAATATTTATTTATTATATTTTACTAAAATTCCAAACTTAAAAGATTTAAAAGAGAAATAAATGCTAAAAACTACACACTAGAAGTTTTTAAAAAATAACAAATTAAATTAAATTTCAAACTTTATAACTCCTATTCTATTATTGTAGATAGTTAAATCCAAAAAAGTTTTCAACAAAAATTAACCTTTTAGTTCAAAAATATAGTTTTTTTTATTTTTTTCAATATATTGAATATGAACCATTTAAAAAAACAATAAAATATACCTCGGAGATTATTAAGTCTCTTTTTTATATTTACTAAATTTAAAAAAGGATAAAAAATTAGAAATGAAAAAATCAAAGAAAATATTTATAACATTATGTGGAATAGCAACTACTGCTATTTTGCCAGTTGCTACTATTTCATGTAATGATGATAAACTAGCAGAGAAAAATGGAAAAGAAAAAGCAGATGCTGCTTTAAAACAAGCAAATGCATTAGCGGAAGAATTGAAAAAAAATCCTGATTATTCAAAAATTTTAGAAACATTAAACAAAGAAATTGCCGAAGCAACCAAATCATTTAAAGAAGCAGGTTCATACGGCGATTATCCAGCAATAATTTCTAAACTTTCAGCAGCAGTAGAAAACGCAAAAAGTGAACAACAAAAAGTTGATCAAGCAAATAAAAAAATTGCTGATGAAAATCAAAAAATTAAAGAAGGCGCTAAAGAATTATTAAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTAATTTCAACAATTGAATTATTAAATAAAAAATCAGTCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACAACTCATGACTTGAAAAAATCGTATCAGAATGAGAAGAAGTTAAAAAAGCTTGATCAAAAGAATTAGCTGAAATTAAATCTGACGATGATAAAAAATTAGCAGAAGAAAATCAAAAAATTAAAGAAGGCGCTAAAGAATTATTAAAATTAAGCGAAAAAATTCAAAGTTTTGCTGATACAATTGCATTAACAATTACTAAATTAGAAGGAAAAAAATTCCAAATTGATGAAACATTTAAAAAACAATTAATTTCAACAATTGAATTATTAAATAAAAAATCAGTCGAAGTTAAAACTTTTGCAACAGTAAATACCATTAAAAAAGATTTCCTTTTATCAGAACTAGAATCATTTAAAGAATTTAACAACTCATGACTTGAAAAAATCGTATCAGAATGAGAAGAAGTTAAAAAAGCTTGATCAAAAGAATTAGCTGAAATTAAATCTGACGATGATAAAAAATTAGCAGAAGAAAATCAAAAAATTCAAAATGGTATCGCAGAATTATCAAAATTAAGCAAGGAAAATTCAGATTTAGCAAAAACTATTGAAGAAACTATTGCTAAATTAGAAAAGAAATTCCAAATTCCAAAAGATTTTAAAGAAAAATTAACCTCAACAATCAAATTATTAAATAAAAAAGCTAACGAAATCAATACATTTGTTTCTACTGTTTCTAAAAAGACAGAATTTGTTCTTGAAGAATTAGAATCATTTAAAGAATTAAACACCCTTCAATTTAATGAAATTAAAACTGAATGAGCAAAAGTACAAGAAGCATGAAAAGACCAATTAAAAGAAATCTCGACAAAATAATATAAATTAAAAATACAAAAATAGTGCTACTAATGGGCACTATTTTTAATTGCTAAAATCGAAAATGAATTTTTTATTAAAAATAAATAAAAAAACGCCCCATATAATACAGGGCCAGCTTAAGCAAAAAACATATTTATTTAATGATAGTTTGCTAAATAATTTAAATATTATTTAGCTTTTTTAATTATGCATTAATAAATTAATTTTAAATATTAAAAATATTAAATATAGAATTTTAAAAAATAAGATAAATAAATGATTAAAAAAATTAATAAAAAAATGGGGCGTTTGATGGGGATCGAACCCACGTATGCCGGAGCCACAACCCGGAGCGTTAAACCACTTCGCCACAAACGCCATTTTGTTAAATTGCTTATATATTTTAACATAAAATACAAATATTATTGTAATTTAGCACCAAAAAATTAAACTCTTGCTTTATATTTATTTTGATAGTCCTTATTAATTAAACAAAAAACGGTTTTCTCCATCCAAAGAGATTTTTTTGAACTTATTGGTTAATTAAATGTGGTTTTTTTTATTTTTTTGAAATTTTTTTTAAAACAAAATAAATAATAAATATATAGATGTTTTTTAGCATGAAAGCCCATGAACATCTATAATAAATTAATCACAAAAATTTATAATTTAGAAACTAAATAAATCATTTTTGATTATTTTAAATAATAAAAAAGGCTTGCTATTATTATGCGCAAGCCTTGTTATTTTTTAATTATTATTAATATCTTGGGATTATATGAACATGAATTCTGAATACTGTTTGATCTGCTGAAAGATTATTATTTATTATTAAATTAAATCCGGTTGCACCTAATTCCTTAACTCTTTTTAAAGCAAGTTCTCTCGCCTTTAATATTAGTGATGACAATGCTTGGTCATCAATATCATATAAATTTGTGGCGTGGTTTTTTACCGTTACTAAAAAATGACCTTTATCTACTGGTTTAATATCTTCAAAGGCAATAATTTTTTCATCTTCATATATTATTTTTGCTGGGATTTCTCTTGTTATTATTTTTTCAAAAATATCATCATGCATATTATACTAGACCTTCAACTTTTAATATATCATAAACAAATTTGTTTGTTTCTGAAACATTTAATTTAAACCCTAATTCATTAATATATTTAACTGCAGTTTTGTATAATGTCTCAAAATTAGCAGTCACTATATTATTTGCAATAATTTCTTGTTTTTCTTTTGTTGCTCATGGAGTTTCATCAAATGTTAAAACTTGTTTTGTAACTTCTTTGCCGTTATAATCTTTAACTTTCTTAGTTAAATATAATGGTGAAATTTTAACTACATATTGGGCAGTAATTGAATTGTCTTTTGTAACATTTATTTTTTGAGGATTTTGTTTTAAATTGTATGCAACATCTTGGTTTATTGAATCAAATTTCTTTGTATTTTTATTGTATTCTAGTTTAGCATTGAATTCATAGAAATTTCTTTTTCCTAAACCATCAACTGTTAAATTCATAGGAATTGTTAAAAAGTTTCCATCAAATGATATTGAACCTTTTTGACTAAATCTCAATGTATATTGCTTACTACCAAGAGTTATTGTCTTTGTATATTGATAATTATCCTTGCTATCTTTTGTTAAACCATCAAATTCGTAATCTTTGTAATCAAACGATTTTAATTCACCTTTTGAAACTTTAGATTGCAATGTTTCGGTTGCTTTTGCCTTTGGATGGTTTATTTCTTGGGCAAGAATTTTTGCAAGTAAAAAGCTTTCTTGACTATAAACTTTTTTGCTGTATGGATCTACAAATCCATATACTGAACTTAAATCTTTCTTTATACTATCTAAATTGCCACCTAAATCACCTGACGTATTTGAATTTTTAACTATGCCTTTATATGCCAATAATGATTTTAATACTTCGCCTTCCATTGAACCGCTATTAATCAATTGTTCTGGGTTTTTAGCTGCTTCATTGTGACCATATTCTGGTTTAGTTGAAGGATTGTAAGAAGCTAGCTTATTAAATCCTTCAATTGAATTTATATATGATTTTGGTCATCTTAAATTCATATCACGCTTATCAGTAAATTCTCAATTTTCAATAATCGGATTTTCAACTAGATATTTCATTAAATACAATGAATCATCTGCGAAATTCAATGCTTCATATAATTCTTTTTCATTAATAGGAGCATCTTTCTTAGACATTTTGTTGTGTAATGCAACTTGTGCTTTGTCTAGACCGTTGCTATCATTTGCTATTTTTTTAAGTTCTGGTCTATTTTTTAATAAATAAAGTTTAGCAAGTAACATTTTTTGAAGTTCTAATCTAATGTTTGCCGCGATTTGATTTGAGTGTTTAAAAATAAATTTAAATTCTTGTTCATTTGGTATTGAAAATGCAGCAGGATTATAGTTAGTTGTGTCAATCTTTGCATTGATAAACAATGATTTTAAATTTCAAAAGAATTGAGGATCAGAAGCAACTCTATTAGTTGCATAATATTTGAATAATTCATAAAGATCTTTTGTTAGCGCTGAATTGCTGAAATTTGAGACAATGTATTCAATCTTGTCTTTATCTTTAGAATCTTTAGCATAGCTTTCTATCTCACTTTTATAAAATTCTGAAATGTATATATCAGTAAGTCAAGTTAAAACTCTATTTTTTGTTAATGAATCTTTTAAAACACGTTTTTGGTACCCCGAATTTTGTTGAGAATCTTCCTTTTCTTTTTTACATGAACTAGCAATCAAAGGAAGAACCGCAAGTGGCATTGCAATAGCTGTTAATAATCTAATTTTTTTAGTCATTGTATCTCCTTTTAATTTTTAGTATTTATGTAATTTACAATTGTTGTGAATAAATAAGATAAGACCTCTTTGTCTTCATGTGGTGATAGAATAAACTTATTGTTCTTGAACACATAATCTTTGTATAAATTACTATTAAATTTTTTAATTATAAATTGTTTTATTTCGTTTGCTTTGCCATTAATTAAAACGTATTTACTTGTTTCGCGAATTAATTTTTCATAGTTGATTGCATTTAAAATATCTTGATCGTTGAATTTACGTTTTGTTCCTTCAAGACTTAAATATTCTTTTGATTTAATATAATTTTTAAAATTATCTTGTTGTAATAAATCATTAACCATATAGTCACGGGTTAATATTTCAGAAAATATTGAATCTAAATTAAACAATTTGGTATTTAATGTATCTGAGTGGTTAGCCTTAGATAGTATTGACAAATCGTTTGTTATCAATCTAGTTACAACTTCTTCTTTGTCTAATGAAAATACATTTTGAATTAAAATGCCACTTTCATTAACTTGAATGTAATTTCCATTACTTTTAATTAATGTTGAAACCTTATACCCACTTGCTTCATCACTAAATGCATCTCGAAATGCCAAACCAAAAGATTTATTAAAATCTTTTTCACTTAAATCATTGATAAATTTTTCAATAGCTGCATTGTATCTAGTGTATTTTTCAGAGAAATTTTCTTTGTCAACACTTTCAGTTCCTTCATTAAAAATATCAGCAAATTTCTTTGGTTTCTTTTCATGATCAATAAATGTGGCATCGCCAAAGATCTTTTTGAAGTTTTCTTTTAATTTTGTAAACAAATCTTTTTTATCTTCAACTTTAAAAATGCTTTTATCTTTGTTAGCATCTCCCATTAAGATCGAAAGAAGATTCATATACTTATTAGGATCTTTTTCTTTTAAACTATTCAATACACTTTTAAAGCCGTCGAAACCATATTTATCAGCTGACTTGCTATCACTTGAAACATATCTCAAAAGGTTGCTATCATTTTTGGCTGCAATTTCTTGCTTTTTAGTAATTTCATTTGTTTCAAGAAGCGAAGATATACCCTTAAATGTTGAAATGGCATCAATACCTTGTTTAATCGAAACCTTATCATCGCTTTCATTTAAATAAGCAGAAAAACTTAATAATTTAATAATTTCTGCCTTTGTTACGACTCATGGATTTGAAGCATCTTTTGGATCTGGATGTGCCAATAAATTTAAATTAGATGTTATTGCTTGCTTTCTTTCGATTCAATTTTTAATGAATTTTAAAGGATTGCGATATTCTTTAACAAAAGATTTTGTAACAAACATCGGAATTTTAACTTCATCTTTTGTTCCAATTTTTAACGATTTATCATCAGATTTTGGCAAAACATAATTTTTATTTTCTATAGCAAAAGGAAGAGTAATTTTTTCACCCTTCTTAGCAATGTCAACTCTTTTGCCTTGATATGTGTAATAAACGTCTTTGTTTGCAATAATTCCATTTTTTAATTCACTATAAGTAAAATCACTATTTACTTCGGTTGTATATCTATTAAATGCATATCTATTTAATTTTTGTGCTAGAAGGTATTCAATTGCTTCTTCTTTTGACTTTGCTTTTCCATATCTATCAGTTGCAAGTTCTTGTAAGAATTTTTCTTCTCAATTTTTTTGTAGACCAAATTGTTCTTGATATTTCTTTTCAAGATCTTGAATTTCTTTTGTTATTTCTTTTTTAACTTCTTCGATTGGTTTTAGTGCTAATTTTTTAATATCTTTTTCTGCCTTATCAGCATTATAAACAGCTTCATATCACAATGAACCTTCATATTCTTTTTCATAAAGGTATTTAATTAGTTGCTTTTCAACACCTTCAGCAGCATGTTTTGATGAATTTGCTATTTCATGGATTTTATTGATATCACCATAGTTCAAATTTAACCTTTGACCATCTGGCCCGGTTATTGATAAAATTGAAGAAGAATCTTTTAATAAAGGTGAAGGCTTAGGTAAGGAATGATTAGCTTGTGCTAACGGAACGCCAATTCCTGCAGCAACTGCGGTTGCTATTGCTGTGCCTCAAAAAGCACCTCAAATAATTTTTTTACGCTTAGCCTTTTGTTCGGTTGTTTTGGGTTGATTTTGATTTGACATAGTAAACCTTTCTATTATTTAATATTAAATAATTATACTATTTTTGGTTATTTTTCTTTATTTATGGATATTTAACCAGTATTATTTTATAATTTAAAACATGAATAAATTACTTTTAAATAAGGATAGAATATTGCTTGAATTTATTGAAATTTGTAAAAATCTTAATCTTTTTTATAGTTTAGATAAATTGAGCTTGTATGGCGCTATGTCAAAAGAACATTCTTGGAATAATTTAGATCACCATGAAATAATGATGAAATATCAAGATTATGAAATCTTAAAAAATGCTTGTCCTAATAGAGTTTTAGATAATACATTGCATTCTTTGTATCACGACTTACAAATAAAATTTGTTGATGATAACAAAAATATATTTGAAAACCAACCGTTTTTGAATATTAACTTAATTTATCCAACAAACATTGCCAATATAAAGAAATATTTAAAACATCGCAATCAAGCTAGATCGTTTATTGGCCACTATTTAACAATGACAAATAGCAACGATTGAAAAACAAAAAATAATATTATTAAATCTCATTTTCTATCAATCTTTTATAAGCCAATTACATACAAAGAAGTTGCTAATTTATTAATAAAAAATGATCACCAAGGTTTTTTGATCTCAAGTCCGATAATTAAAAAAGATTCATTGAAATATTGATTGCCAAATATTAAATTTGAAACAAAAAAAGTAATTATAAACAATATTGAAGTTGATGTTTTAGTTGAATTTGACCAATATCTAAAAAACGTTTATGAAGTTGAATATTTTGAACATTCCGTTCCACAAAGAAAGATGGAATATATTAACCCCATTGAATATAAAAAAATATTTAGCTAATTTTTATAAAAGAGAGTTCACAATTGCTTTTTATCGAAGAAATTTTAGAATTTCTAATGTGCATTGAAATGTCTTTGTTTTTTAAAAAATAAGCAAATAAAAAAGTAGGAAGTTTCTACTTCTTACTTTTTTAAAATTGACTTTCTCTATCGATTAATCATTTTCTTTCTTGAAGTTTTACTTCTTTAATTAATCCCAACTCCAAGTATATAACTTTATCAGCAATTTGGGCAACGTCAGGGTCGTGGGAAACAATGATAATGGTTGTGCCATATTTTAAATTGATTTGTTGCAAATTTTTCAATACAATAATTCCTGTTTTTTCATCCAATGCTCCTGTTGGTTCATCAGCTACAAGAATTTCGGAATTTTTTGCAATGGCTCTTAAAATTGACACTCTTTGTTGTTGACCACCGGACATTTGAGAAGGATATTTATACATACATTCAGAAAGATCAAAATCTTCAAATAATTTTTTAATATTTAAACGCTTATTTTTATCTCTTTGTAAATATGAACCGGTTTCAACATTGTCATAACTATTCAAATTTTGAAGTAAATTATAATTTTGAAAAATGAACGAAATATTTTCTCTTCTAAATAAAGTTTGCTTTGCATCAGATAAATAAGGCAAATTTATGTTATTTACAATTACATTGCCAGAGGTAGGCCGGTCAAGAGCACTAATTATGTTTAATAATGTTGATTTACCTGACCCAGACTTTCCATATAGAACAACAATTTCTCCTTTTTTAATTGAAAAAGTTATTCCTTTTAAAACTTCAGTTGCTACATTGCCGGATAAATATGTTTTTTTAATATTATCAACTTCAACAATGTTATCGTTAGAATTTTGCAATTTAGCATACTCGTCTTTTTTTCTTGGTTTGTCATTAGCAAGTCTATATTTCTTTAAAGTTGCATTATCTAAAACCTTAATTGCAATGCCTTCGTCTTTAATTTCAAATGGCTTTTTTTGCTTAGAAATATTTATCAAGTTCAAATCTTGCAATGTTTGTTCATCAACAAAATTTTTATTCTTAGAAATTTTATTTGTTTTAGCTTTAATTTCTCTTTTGTTTTCTTTTATTTCTTTTGCCATTTTATTTTCCCTTCAAAGCATCTACTGCTTTTATTTTATTTAAACTATTTCATGTTGCAATTGATGTTGCTATAAATACTGCCAAACAAATTAATGCACTTAATATTACTGTTGAAATATGTAAAACCAAAGGTAATACAATCTGGCTAGTTACCATCATAAACGAGTTAAATATTGCTATTATTGCAAGAGTTGCAGGAATCATTAGTAAGCAAGCAAATACAATTGCAGGTACAAAATTGAAGAAGAATAATAGAATTTTTTCTTTATTTGTATATCCTAATATAGAAAATGTAGCAATTGATCTTCCATTTGAAGCAATCATTATATTAGTAATAACAATTAATATAACTATTGAAATTATCAATGAAATTATTATAAATGCCAGCAATATGGTATTAACTGTTGAAGAAATTCCTTCAATAAATCCAGCTTCAATGTCTTTACTATTGACATCAAATGATGCACCATACATTATATTCTTGCCATATATTGAAGATGCTTGTGTGCCATAGAATTTAGTTAATGTTTCTCTTACTAAGATTTTATAACTTTCTAATGGGCTATCTGAGTCTATTATTTCTTGTATGTTTTCATCGTTTAGATTGCCAAGTAATTTCTTTAATTGAGTTTTATAATCTAATTTTGCCTGAGGATGTGCTTCATTATATGATTGAATGTTATGTTCATATACAGAAATATATTTCAATTTTGGATTGCTTATAAACATGCGTTTAAAGAAATCTCATGCGCTGTTGTCATTTGCTTCGGCAACATCATATGAACTTGCTGCACCTCAGAATCCTGTTGCTGAATAAGTTGTTAATGTATCAATAGTTTGAACTGGTACTTTATCAATTGATAAAATACCATTAAATGCATCATATTTTTTATTGAATTTTTTAATATATTCATCTTTTAAATTAGGGTGTAAACTAATTGCTTTTTGTAATTCGTATTTTCTTGCATCTTTTAATCTTTTTGAAAGAGTATCATAGCCTAAAATGTGGTCTAAAATATCCTTCCTTGTACTAATTTCAGAGTTAATATAAGTATCTGATATGCCAATTACTTTAAACTTATAATCGACTCTTGGGCTTTTTTGCATTAAAGCTTGATATGCAAATCGATCAACGTGATTTAATATTTTGGCATCAAATACGGAATTAACTTTTAATCTATAGCTTCTTGCCGTTACTTCATTTACAACAACAGGAATTGGATCATTACTATCAAAATTGTAATTATAGTCTTGTAATAATTTTGTTAAATTATTGCCCCGACTATCTTGCAATCTCATGAATTTACTATCATTATAATATCCATAAATTTTAGTTTCTTTTCCATTTAGAGTTACTGAAGCATATGTGTATTTTTCGTTGGTTGTATCGTTTCAATAAACTCCGCCAAATGAAACATAGAAATCTATACCATTTATTTTTCTATAAGCATTTACTAAGAAATTACGATATTCTTGTCTGAATTTTGCTGTACTTACTTTGGCGGGTTTTAAATAAATTTCATCTTCTGCAACTCATTCAACAAATCTAAATTGTTTTCTATTTCCTGATGCTGCTTCTGAATAATTACCAGCGCTAAACAAGAAGTAACTTGTTCTATTTGATAAATCTTCTGGACGTTTAGCATTATAGTCATCAAAGAATTTTTTAATATCTTTTACAGCAATTATTTTTGTTGGATCTTTTACATCTTCTTCATCTATTATTCTATAATCTCCACCATTTTTCAATCTACGTAAATTTATTAAGTTTTGAGTTGATTGCATCATTGAACTTACGCGGTTAAATATTTGGTTTACTCTTGCCCTTTGTGTTTCAGGCATGTTGGCATAAGTTATATCTCATGGACTTAATTCAACTGATAAATCCATTAATAAATCAAGCGATGATTTTGTAATGACAGTAGGCGCAAATTTTCTTTGAATTACATCTGTATTAAATGAAGCTCCAGGTCTTAAGAAATTAGGATTATCATAATCTAGTTGACTACCATTTGTTGATGAATCACCTGCAAGATCATTAGGAACATACAATAAATCATTTATATCGTTTTGATTGTATGTAACATAAGGACCACCTTCGGTTGTTGGACTTTCAAGATTTAATTTATATTTATATAGCCTATTTTTATATGTCTTTGAAATAGTTTTATTAAATACGTTATTTGAAGATAAGAAGAACATAGAAATTAATGAAGTCGTTGAAAATGCTAAGAATAACGAGAACATTTTTCAGAAATTATTCAATGCCATCGAGGCAATAAATCTTGATTTAACTGGCATTTTTCTAAACATAGCAGAAACTCTTTGGGCAAAATTACCAACACTAACTTCAGTTAACCCACTCATTAATTCTGTAGGTTTATTTCTTACAATAACTCTAGTAATTATATAAATAACTAGTGAAACAACAATCAATGGAATAATAACTACTGCTAATATTAATAATATATTAAATGGAATAATATTGTTCTCTAACGTTCAATAAGAAGATAATATTGACATTGCAGGTTTTTGTAAGGCAAATCCAACAATATAACCAAATATTCCACCAACAATAGTTGGAATTCAACCAAAAGCACAGAATGATAAAGCAATTTCTGATGTTTTATATCCTTGAGCTCTTAATATTCCAACAACCTTATTTCTTGCTTCAATATATCTTTTGATAATAAAGTAAACAATCAAAATTACTAATATTGCCAAAATTGTTATTAAATAAAGTGTTGCATTTTTAATTGATGTTACTAATGTTCTTATTGTTTTTACTCTGATGTAACGTTCAGGGTTGATTGCATCTGGTTCATCTTTTAAAAATACTTTATTTTTTGAATTTGGAGAAATTGATGAAACAATTCTTTGAAGTTCTTTCTTTAAGTTTTTAGGATCTTTGCCAGCTAAATATTTTCCTTTTTTGTCAACAGGAGATTTTACTAATGCATAACTCTTTAAGGCAAATGTAGGATATGCCGATCTAATTCTGTCAAAACCTCTTGAATTAACATAAACAACAGATTGAGTTTTTGTATCAACTTGTAAATTTTCTTCATTAATTACAGGGTATAAATAATCAGCGGTGCTATCAATACCTGTTATCAAATATTCTTGGGTATTTACTTTAATTTTATATTTATCATCTAATGAAGCAATAAATTGTTGCATTTCAAATGGATTATTTAAATATTTCGAAATATCGCCATTGTATACTTCTTTTTTATTTTTGTTTAAATATCCATAATTTACCTTGGCTAAATATGAAGCAGCTTCGGAATATGCAATTAAATTATAAACTGTGCTTGGTTCAATTATTTTTGAAAATACTTCCTTAACAGTTTTGGTAATATTTATTCCAAAAAAGCTAATATCTTTGTTATCGGGTGTTTTTAATAAATGGTTAAACATATAATTGAATTTAGAAATATCTAAATCAATTCTGCTATCGCTTTTTGAAGTAACAACATTTTCAACAAAATTGAATTTTTGAGTTGCACCTTCGATTTGTTTTTTCAACATTGTTGCATTAGTTTCATCAATATTCGATAATAATTTTTTGATTATATAAGCAATATTTCCACTATCTGGGTTTGGACTTAATACGTAGTATAACAATATTTGTGGGAAACTTAAAATATTGAAACCATTTAGAATATTTCTTGGTACGTTTAACAACGAAATTATTGAGGCGGTTTTTGAAGCTGCAATTGCATCCTTATATACTAAGCTAAAGGTATATTCATCATTTATTAACGGTTTAATTTTTTGATTTTGATCATTGACATTAATAAATGATTGTAAAAATAATTTTAAGGCTTGAAGATAATGAGTTGGCATTTGATTTGAAGAGGCCATATAACCAAAATTGTAGGTTATTTCATTCATATTATTTTTTAATATCTCTTTAATTGCTTCAATGCCCTTATTAGAAACATGATTTTTAGATAATTGGTCAAATAAATTTTTAAATACTGCCGAATTATTCATTAAATCCATAATTTGCTTTGTTGCTTTTGCTGTATGCGCGTGAGCAAAAGGCATTTTAAATAATTTAGCAGAATAGTCATCGTCCGAGCCTAAAATTCCAAGACTAGGAATTGGGTTAATTACTTTTTTGTAATTTAATACTATATCTTTAATTTCTTTTGATGTTCCTATGTTGTAAAGACCTAACAATATCTCTTTAATTTGCTTGTAATCTAAATATTTTGTAACTTTTCGTTCAATTATTCTCTTATTAGTTGTTGGATCAATAACAATATTTTCTTGAATATCTGCAATTTGGCTTAGTTGATATGCAAGTTTAATTCATAATGAATATAGAGATAAGACATTAGAAGCAACATTTTGTGGATCATTATTCAACATTAATTTTGCTAAATTTTCTTTTATTAATGAATGAATAGTTTTTAAAATTTCCAAATCTTTTGCATGTTCAGGCGCTACATTATTAAATGAAGCTGAATAATATGCAACATCCAAATAAGATTTTAAATCATTTAATTCAACTGCTGAATTTATACCGCTTTGCTTATATTGAGAGTTTGATGTTACAAGTTTGTCTATATATTCATGATATTTAGTTAATTTTGAAATTATTAAATCAAGGTCATTAAATTCGCCTTTAGTAACTAAAACATTGTTTTTTAATACATCAAATTCGTTTTGAGTTAATTTCAACTTTTTGTTATTTATTTTTGCATCTTTTGCCTTTAATAATATTTTTTTAATATCATCAACATCAAAAGGATTTATTGGAATATTAGTCAATACATTTGTTTGCTTTTGAGATTGAGGGAATGAAAATTTTGACATGTTTATTAAATCAAACAAAGTAATTTTATTATTGTCATAATCAGGAATAGTTATATTCAATTTACCTAAAATTCTTTTTGTCTTATTTGAAAAATTAAACATTTGAATAACGGATTCTTGAATTTGGGAAATTTTTCCGCTATTTATTTTTCCATCTAGGTTTGAACTTATTGCACCAAAAAATGATGTTAAATAATCTGAGCTAGTTAAACTTTCTGTGTTGAAATCTTTATAAATTTTATTATTTGCAACAATTGGATAATGTTCATGTTTAACTAGTTGCTCCAAAATTTGAATAAATCTATCAAGATCAAAATTAGCTATTATATTTTTTAATCCTTGCTTAAAATTATCGTATTCTTGGCCTGCTTTTGTTGCACCGTTTAATTTTCTAAAAAATTCTTTAATTCCATCTTTTGTTTGTTGGTCTATAAAATTATTAGCTTCTCTATTTGCATTAATTCATTTTGAATACAAACTATTAGGCGATTCTGGATTTAAAAAGCCATTAAAGTCAATATTATCAATCAATTCAGACAATGTTGATTTTAATTTGTAAACATCAATAGATTTTAAGAAACTTATTAAAATTCTTTCCGATGATAGTATTCAATATGTATCATCAACACTAGTAAAAGGCTTATATGGATGTTTTTCAAATCAATCTTGAATAATTAAAGAAAATTTTTCAAAATCAACAGAGCGAATAACATTTCTCAATACTTCAAAAATTGTTGATTTATTTTTAACTAAATTAATTATGTCAAATAAACTTAATTTCAAAGAAGGAATCAAATATAGATTATCAAGATTTAATAATTTACTTATATTGTAAACTTGTTGCATTAAATATTGATTTCGTTCATTTTCATTGGTTCCTGAATTCATATACATTTTTTCTAAATAGTTTATTATTCCAATGAATACATTTTGAACAAAAGCATTAACATTAACATTATTAATATTTAAATTATTGCTATTGCTTCTATCTACCGGTCTGATAATATATTTCAAAATATCTAAAAGAACTTTTTGCAAAATCGTGCCATTTACCTTGCCAATATTTAGCAATGTATGGAAATCGTTATCCACAACACTTTGTTTTGCTGCGTCCATGATTCTTAATAAATCATTTTTATTGAACATGTTAATAATTTCGCTTTTGAATAAAGCATCTCTAATAACATTAACTAAACCATTTATTGTTTTATTTTGAATAATTTCGTTGGTTAAATTGTTGTTTATTGCCCCGAAACTTATAGGCAAACTAAATGAGTTTTTAAACTCCTTATCAGCAACTGCTCAACCATTTGGGCCGATTTCTCCTCTAATACTATAATTATTTCTAATCATGTATTCATAAAGTTCTTGAGATGTTAAATATGCCTTTCCATTAACGTCAACCCTATTTCAAACTTTTTTATCACTAAATCCATCAATAGTTGAATATTTTAATAAAATGTATTTTTCATCACTAATTCTTGCAACTGCACCAACTATTGAAGAACCAACGGTCTTTGGTTCATCATTAGCAACAGTCAACATCAATATTTTTCCATCGATTTCATAAGGTATTTTTGTGTTTGGCAAGAAATCATAATATTTTAGAAATCTGATATCTGCATTGAAATAATTAATAGACGGCGTTCATTGTTCAAAAATATATTTAATTATTTGTTTTGTATAAACTGGTGGAATTTTTTTGATCATTGGATCGTTTGGATCGGGTTTTAAAATAAATTCATTAACATTTTTATCTGATACAGAAGAATTTAGTAATGTAGGATTTAGAGTTTCGTTATATAATTTACCAACGTTATTTTTTACGCCATAAATTTTTTGGTTTCCATCGCCTGCATTAACAAAGTGAAAAACGTTTTTCTTAGTAGTTTTTTCATCAACGGATTCTACAGTTAGAGTTTGTCTAATTCCTAAGTTTTCTTCTCCAACATTATTTTTTATTTCATTAACTAAGGCATTTCTAGCATATGAAACAGATCCTTTTCTAATAAATTCTTGATATTTTTTTAAATTATTAGGATCCGATATTGCTTTAAATTGGTCTTCTGTAATTAATTTATCACCGGATTTAGAATACTCAATTTGTTGAATGTTTCAACGGTGTTCTTTAGAAATGTTTTCTTTTATAGCAGGAATTTTGGAATTATTAAATCCACCATTTCAACTGCTTTTAAAAGTTTGCAATAAATCTTTTTGTTCAATTGGCAATGTTGTAGTTTTTTCTTGTTTCAAAGAAGACGAATTTAATTTATAAATTGTTTTAATCGTCTTATTTCAATATGAAAAATATTGAATATTTTTATAAACATTTGGGTCACTTTCTTTTAATTTGGCAATGTATTTGAAATATGATCCTTTTCAATTTGAGCTATATTCGTCACTCTTTTCGTTTCAATTTAATGCAAATCTATATCAATTATATGTTGTTAAAACCTCAGTAGATTTTATTCATTCATTTGGAATTTGATATATTTTATTTGCTGCTAGTTTTGTAAATTCATTAACATCTTTTTCAATACTTTTATTGCTTAAAAAATTTTGAAGTTTAAAAGTTCTTGAAATATTAAAGTCTTCATTAATAAATTTTGATTTTTGACTTGTAAAATCTGAATTTTCAATTTTTAAATCAGAATTTTGATTATAAAGAGATGCATCAAATTCATAATGGTTTTCTGAAATTTCTTTAAAACCAAGCATCTTCATTACATCTTTACCATCAATAGTGTGCAATGAACCACTATTTTTTCATGCTTCATAATCTTTAGTATCAAATGATGCTTGTTTAGTTTTAACATTTAGAAATAATGGAGAGGGATTGTATAAATAATCAACTACTTTTTGATCGTTTAACGAGCCTCTTGGACCATATGCAATAGAAGCTATATTTTTTAATCTAACTTCATTTAAAAAAGTAATGTCATCATTTTTTGTCAAATTATATCTTTTAGTTAATGGTTTTATATTTCCTTTGTTATCTTTTACATAAAGTTTAAATTCCCTATCATGACCCGAAAAACTAAAATTCCTAATTTTAGAAAAATCAGGGTTGGATTTTAAAATTTCAACGTCTACATCACTTATAGATTCCTTGCTAGAATATAATAAATTTAATAAATCATAAGTATTTATAAAATGGTTTTCATTATCATAATTTAAATTATTAAAAGTATTTTTTAAACCTACATAATTTCTTTGATCTTTTGGTAGATCTATTGAATATGAAATATCACTTCCATTATTAGAAGACTCAAAAACAACAGGTTTATCAACAATTGATTGATTATCAGATCCTATTTGGTTATAACCTGATTTTGGTATCACACCTGAAGGATTAACGTCAAAATCAACAGTTGCATCATGCAATTTTGAAACTTTTTCATACGAATTAATCGTAGATGAATAGCTAGTAGTAACATCATTTAACAAAGTAAATAAAAATGTTGTTAAGAAAATTAAAATTGTTAAACAAATCAAGGTAACTTTATTTCTTGCCAATGATTTGAAAACCTCTTTAAAAAGTCTTCTCATATTTCTTCTCCAGTTTAAAAAATATAATTTTTTAATATATTAAAAATATAAAAATATGTGTATATTATAAATGTTTAAATCTGAAATAACAAAAAAACAGCACTTTTAGACCAAAAAATGGCTTAAAAATGATGTTTTTTGTTAATTATTAGTTATTTTTTAGATTATTTTTAATAATTAAAGATCATTTTCTAGGAAATCCTGAGGCTGTTTTTATTAATTTTTGGTGAAAAATTAAAACATTTTCAATATTGAAATATATTGGCAAAATTCTTGTTTTTAAATTAATATTTAATTTGTTAACTATAAAGTTTGAATTAGCTATTTCTTGCTGATATTTATTTGATTTTAAAAAGCAAGCAATTCCATTAATTTTTAAAAGATGGTGTGAAATTTCTAATAAATTTTTTAATTCGCTCACCGCTCTTGCACTAATAAAATCAAAACATTCTATTTGATTTGAATCTTCAGCACGAATTTTTTTTATATCAATATTTTCCAATTTCAACTCATTTTTAACCAAATTTAAAAAATTTATTCTTTTTGATTGGGGTTCATAAATTGTTAAAGAAAAATCTTGCTTATATATGAAATATGGAAGGATAGGAAATCCTGCGCCCGAGCCAATGTCGAGTACAGATTTGTTAGTTAGATTACAAATATTTTTTTCAATGTCTAAAAAAATTAAAATTGATTCAATTATTGCTTCTTTTAGAAGCTTTTCGTTATAGAAACCTGTTAAATTATATTTGCTATTTTCTTGTTCTATTAATTGATAATATTCATATAATTTTTTTAAGACTTCTTTGCTACATTGCGGTAGACTTTGCAATATTAAATTATTGCTTTCTTTTTCCGTAAGCATTATATACTTCTGTTATAGATTTGCTTTCAATGTTTGCATTGATTACCATACTTAAAAATTGAGCCAAAGATAATTCTCTTAATTTTTTAAAGTTTGATAAGTGGCTGTGATCTATTGAATTAGTAATAATAACTTCATCAATCATTGCGTTATTTTCAAACATTTCAAACCCTTTTGAAAATAAACCATGAGTAGCTGCAATTATAATATTTTTTGCACCGTTTGCTTTTAACGCTTCTGCTGCATGAATAATTGTTCCACCTGTGTCAATAATATCATCATAAATTAAAACATTTTTATCTTTAACATCACCTAAAACACCCATAATTTCGCATTTATTAGGTCCTGTACGACGCTTGTCAATAATTGCAATTTGTTCTTCATTGTTTAGTAATTCTGACAATTGTCTTGCTCTAACTGCACCCCCGTGGTCTGGTGATACAATTACTAGTTTTTCTTTTCTGTTTAGCAATTCATTTGAAAAAATAAATTGTCCCCTTAAATCATCAACAGGAATGTTGAAAAATCCTTGAATTGAAGCGTTGTGCAAGTCAATAGTAATTATTTTATCAGCACCCGCTGTTTCATACATCTTAGCAACAAGTGCAGCTGATATTGGATCTCTCCCACTTGCTTTTCTATCTTGTCTTGCATATCCATAGTAGGTCATAACGCAATTAATGCTCTTTGCTGATGAACGTCTTAATGAATCGATTAAAATTAGCAATGACATTATGTTATCGTTTACTGGTTTTGAGGTATTGCAAATAATGTAAACATCCTTATTTCTAACGGTTGTTTCTGATTTTAAAAGGCATTCTCCATCTGCAAATACTGTAGTTTTAACTGGCAATATTTCTTTATTTAAATACTTTCCAATTTCTTGTGCTAATTTTTGGGAATTTTCCATGCCAAAAATTACAACATTATCTTCTTTTTTTGCTTTCATTTAAATCCTCATATATATTCTCTTTAATTATTTATAGTTAAATTTTACATTATTTTATTTAAAAATAAAATATTAATAAATTGCAATTAAAAAAGTGCAAGTTGCACTTTTATAAAAATTCTTTATATATACGTTTATAAATTGACTTAACACTAACAACTAATAAGACATATATTCCTAAAAATAAAACTAATCAAGCATAATAAATTCCTGGCAAAGGCATTAGACTTAAACCTTTATTAATTCCAGGTATATATGGCACAGCATTAATTATTGCACACCCGATTAATGTTGAAATAAATAACGAGGTCGTTGCATTTGATTTAATAAATGGTGTTTTATCAGTACGCAAAAAGTGAATTACAAGGGTTTGAGTATACATAGAAACAACTAATCATCCTGCTCAGAATAGTTCTTTAAATGCTTTTTGTTTTTCTATACTTAGTTCATCTTTTAAAGAAGCAAAACTCTTATATGCTCCTATTTGTACTCAATCTCCCTTGCTAGTCATAAATGACGCATTTGGAATTATTGCAAAGAATAAGACTACATATGAAACAATATCTACTATTGAACTCGTTGGACCAAACCAAAACATAAATCTTCAAATTGATTTATACTCTCATTGCCGAGGTTTAAGAATAAATTCAGGGTCTACATTATCTCATGGCATTGTTGCACAGCAAACATCATATACCAAATTCAATAATATTATTTGTACAGGGTTTAATGGAACAAAAGGAATTAAAAATGAAGCAATTAAAATACTAAATATATTTCCAAAGTTTGAAGATGCAGTCATTTTTATGTATTTGTTAATATTGGCATGTGTTTTTCTACCTTCAACAATTCCTTTAGCCAAAACGTTTAGGTCTTTTTCTAAAAGAATTATGTTAGCTGATTCTTTTGCAATATCAACTGCAGTATCAACTGATATTGAAACATCGGCTGATTTCATAGCGGGTGCATCATTAATTCCGTCACCCATATAGCCAACAACATGACCATTATCACGCAATATTGAAATAATTCTTGCCTTTTGGTCAGGGCTTAATTTTGCAAAAATTTGAACTTTTTCAACTTCAATTTTAAGTTCAGCATCGCTTAATTCTTCAACTTCTTTGCCTAGTAAAATTCTATTATTTTTTATTCCTACTTGGGCACATACTGCTTTTGTTACAAGCGCACTATCTCCCGTTAATATTTTTACATCAACTCCATATTCATGCAATTTTTCAATTGCAGCAGCAGCAGAATCTTTAGGAGGATCAAGAAATGTTAAATATCCAATCAATGTCATATCGCATTCATCATTTACCGATATTTTTCCAACTGCATTAATTGTTGATTTTTTAGAAGCGATTGCAATAACTCTTAGTCCTTCTGAATTGAATTCTTCAACTCTTCTTAGAACCTTATCTATTGTTTTTTTATCAAGAGGTTTTACCTCTCCATCAATGTAAATATTTTTACATACACTCACAATTTCTTCGACAGCACCCTTGGTAATTAGGGTTACTTTATTTTGTTTTAATGATTTAACTAAAACAGACATTCTTTTTCTTTTGAAATCAAAAGGAATTTCGTCAATTTTTTCATAAAATCTTTGAAAATCCTCAAGTTCTTTGTTTTTTGATGCCATTTCAAAAGTCTTATTAATTATTGATTTATCAAGTAAATTTTTTAATCCTGTTTGATAATATGAATTAATAAAAGCATATTTTAAAACATGAAAATCTTCTTTGCCGGTAACATCCATATGACGTTCCATAATTACTTCATCTTGTGTTAATGTCCCAGTTTTATCTGTACATAAAATGTCCATTGATCCGAAATTTTGAATCGAATTAATATTCTTAATTATTGTTTTGGCCTTGCCTAGTGAAATTGCACCTTTAGATAAACTTGCTGTCACTATCATTGGAAGCATTTCTGGTGTTAAACCAACAGCAATTGATATACCAAACAAAAATCCTTCTAATCATTGATTTTCTCCACCTATAACACCTTTAATTCCTAAAATTAAAAATACTATAGGAACAATAACAGCCATTATAATAGTGAACATTATTGAAATTTTTTTAATTCCTTTATCAAAGTTGGTTGGTATTGGTTTTTCATTGATTTTAGAAGCAACTTGTCCAATAATTGTATCATTGCCGGTTGCAATTACAATAGTTTTAGCAGAACCTGAAATAACATTGGTTCCCATAAAAGCTAAATTGCTACGATCAGTTATTGCTTCAAATTCTTTAGAACTGTCGATGTTCTTATATTTTTCAATAGCATCGCTTTCTCCGGTTAATGAGGATTGAGAAATAAATAAGTCTTTTGCTTCTAATATTCTTACATCCGCCGGAATAATATCTCCAGCTGCTAAATACACAATATCGCCTACTACAATCTCATCCATTGGAATTTCCTTCATAATACCATTCCTTTGAACAAGCGAAGTGGTTTCAACAGTCTCAACTAATTTATCAGCGCTTGATGCCCCACGTTGTTCTTGAACGAAGTGAATAATTCCACTTAAAAAAATCATAAATAAAACAATGATGACAGTCATTCCATCAATTCATGTTATCTTTTTGCCTTTTGATGCAGGCAATATTAAATCAATAATCATAGAAACAATTGCCAATGCAAACAAAATGATGTTAAAAAAATTAAAAAATGATCTAAATAAACGCTTTCAAACATTATCAGCGCCTTTTTTTGTTATTTTATTTAAACCATATTCTTCTCTTGCAGCTTCAATTCTTTCTTCATCTTGAAATCCGTTTTGAGAGGTTTGATATTTTAAAAATAATTCTTCTTGACTACAATTAGAAGCTTCAACTAACCTTGTATTGATTTGCTTAATTTGTTTTTCTTTTCTTGAAGAATTTTTAAGTTTATTTTTTGTTATATTAACTATCATGTTATCTCCTTTTTATTTGGGTATTTATTATAGGAGAGCGAAATATAATTAAATACTAAAAGTTACTATGTAATTTTTAGTTAAATTGCTATATCGCTCACAACTTGGTAATTCCATAGTACCTCCATATATTTAAGTTTATAAAAGTATTTTAACATATTTCAAAATTAATTTCATCTAAACTTAAAAATTAAAAATTATATAAATCTTTTATTTTTAATTCATTTAAATTTTTTGTTTCACCAATTTCAATAATCTGTCCATCTTTGACATAAATGTATTTCTTAAAATAAGTTTTTATTAATGAAATGTCGTGAATATTTACTAAAATTGTTTTTTTGTATTCTTGTGATATTGAAGACAATAATTCTAAAACATCTCTTGCGTTTGCTATATCAAGACTATTGGTAGGTTCGTCTGCTAAAATAATGTCAACTTTTTGTAATAAAATAAGTATCAACCACAATCTTTGGCTTTGACCCCCGCTTAATTCATCGACTCTTGTAAATAACTTATCAAATATATTGAATTTTACGAAATATTCATATATTTCTTGTTTTTGAGTTTTGCTTAAAACATGAAATCAAGAAAAAAGAAAATTTTTATATTTTGGATAAAAATGTAGAATGTTTTCATAGCAATTTAAACTTTCAATAAAATTATTGTTTTGACTTAAAAATCCAATGTTTTTTAATATTGCCTTTCATTGTTTTTTTGAAGCACTATAAATATCAATATCATTAATTTTTACATTGCCTTTTTTTACAAACAATTGCCTCAATAAAGCATTGAAAATTGTTGATTTTCCAGCGCCGGATTTTCCTATTATTGCTATAAATTCACCCTCTTCAATAGAAAAATTTATGTTCTTTAAAACTATTGGACTTTTTTTATAATAACTTGCATCAACATTGATGAATTCAATTTTTTTCATATTTAAATTATATTTGGAATGTATTAATACAAAACAAAATATTGCAAAAATAAAAACATTAAGCGTGTTCTGCTCAATGTTTCTCAGTGAGGCTTGTATCGACCTCATTAATTTTTATTTAATAGTAATTTCAACATCCAAATTTTTTAATACTTGAATTGCTGTTTCTTTAATTTCTTTTGAATATTCATCAAATTGTTGTTGTTCATCTTCGTCAAGTCTAATTTTTGGCAAGTGTGTATAACCATTCTTACCCAAGATTACTGGTAAACCAAAGTAAACTCCGGTATGTTTATATTCCTTTGGTAATTGAACTCCAAGAGAATAAATTCTTTGTCTATCATACAAAATTGCTTGCGTAATTTCGGCAAGGTTTTCGCCAATACCATAATTGGTAATGCCTTTACCAGCAATAATTTCAAATGCTTCTTTTTTAACCATTTCATTTAATTGAGAAAGTCATTCATTAGTTATAGTACCATTAGCTAATCAATCTTTCAATGAACCCAAACCAATTCTTATTTTTGAAAATGGAATTAAACATCTTGCTCCATGTTCACCAATAACAAATCCAGTAATTGAGTGTGCTTTAATTCCAATTTTTTCTGAAAGGAATTTTTTCATTCTGGCAGTTTCTAGAATAGTTCCTGAACTTATAACTTTATTTGGAGAAAACTTGGTTACTTGTTGATAAATTGTTGCCATAATGTCAACAGGGTTTGAGGCAATGATTGTAACACCATCAAAACCTGAATCTTTTATGCTTTGGCCGATTTTTCTCAATAATTTTGCATTATCGGTCAATAATTCCATTCTATCTTTTAAAACGCCTTCTTTTGGTCTAATTGATGCAGTTATAATAACAATATCAGCATCTTTCAAATCTGAATAATCTCCAGCTTTGAATGTTGAACCATTGTTGGCCATTGAACAAGCAGCATCATTTAAATCTTTTGCTTGCGCAATTGCGACTTGTGGAAAACTATCAATTAAAACATAATCTGCTTCTATATTTTTATTTAATGCAGCATATAAATATGAACTACCAACAGCACCAACGCCAATTAATCCTATTTTAGTCATTTTTTTTTGCCTCCTAATAACTCTTTAAAATTTTTTGCCAAATTAAAATTGTAAGATTCCAAAGGCTTTCTTTCGAATGGATTCTGTTCATTTAAATAGGCAAATAAAATACTTGAATGATGTAAGAAAGACATTATTCAACCCAATGTACTATCGTTATAATTGTCAATCATGATTTGAATGTTAGGTATTTTATATACATTAACATGGTTTTCAATAAATGTTTCCATTACAAGATTACATAAATTATTATAAGTTAAATTGCTTAAATAATTCAAACCATCCGTCTCATTTTCGTTATTTTGTGAAATTTTGCAGTCCATAATAGGTTCTTGAAATTTTAAACAAGTATCAAAGCACTTAAATGGAGTGTTGGTGTAATTTGAGGCCAAAGTTTTATTATCAACGCCCCCTAAGCAAGTTTCAATATAAATCCTATTGTTCATAGATGAAATGGTCTCACTGAAAAGCATTTGCCACAATTTTGCTAATGATTCTTGATTTTGTTCGAAATAGCAAACATTTTCAAATATAAAATTATTATCATGAAGGATTTTTCTAGTGATTGCATATAAAACTGCTGAGTTTTTTTCTAATGGTGCATTTAAAAAAAATTCATTAGCCTTATGAGCACCAGATAAATATTTTTCAATATTAATTCCTATACAAGCAATTGGCAATAAAATTGCAGGTGAATAGCTTAAGAATCTTTCGACTGTATTATCTAAAATTGTTAAATGAGTATATTGATTTATCTTGGCATCATTAAATAATCTTCCATAATTATTGTTTGTAGTTACATAGATATATTTAGAAGCTCCCGCCTTGCCTAACTCTTTATATAATAAGTTTTTTAATTCTCTAAACATTATTAAGGTTTCTAAGCATTCGCCAGTTTGCGAGATTACATTAATAGCAAACTTTTTATTTTGAAGTTTAATAAGCAATTTGGCAACTTCTAAACTATTAAAATTTTCATTAACATAAACCAATTTAATTGATTCATTTTCTTTTCTTAAATCAAAGTGTAGATCAGCAAGCGCCTTAATTTGATTAATTATTGTTTGAGTTGCTATTATTACTAAATATTCAATACCGTTCGACAATAATTCACTTGATATTTCTTGAATTTTTTCATAATCAGATTTCTTTACATTTTTAAAAACATTGAATACACCTAAATAATTTGAATTTTTAACCTTTGAGTTTTTTATATCTAACAAAATTTTTTGAACCGTCTCATAATAACGGCTTGGTGCACTTTCTTTAATTGCTTTTTTTAAATTTATGTCAATAATTTGCTTATTTTCCATAATTAATTCCTTTGTTAGTAAATTCTAATAATCCATCAAATTCTGCATCTTGACTATCTAGTTTAAATTCAAAAGGGTTTCTTAATTCCTTGGGGTGAATTCTTTTAAATGAAACTACTAAATCATTATTTGGCATTATTTCAATTACTTGAACTTCCTTAATATCATTGATTTTAAACATTAATTTTAAATCACTAATGTAATAATCACTAACTTCATTTATACTCAAAAAGCATTTGCATCCTTCTTTTGTTTGCAAAATAATATTATTCTTTCAAACCTTTATTATTTTGGCTTTAAAAATATCTCCTATTTCATAATTCATTTTTGCCTCTATTTTTATATATATTTATAATAAAATTTCTAAATTATATTTTTGTATAAGTTATTAATTAATTTAATAATCATGAATATATAAATTTAAAATTTAATATATATTAATTATTAATGCTTAAATTATATAATATATAAAATTTAAGTTTATACATTTAAAATAAAATGTAAAAATTAATATAATTAGTTTATGAAAAATAGTTTTTATAAATGCTTAAACATTGCAACATTGACAGTTTCCTTGCTTTCAACTAGTCCTATTTTGATGATTAGCTGTGATATAAAGAAAGATAATGACTTATTGCATTTTAAAATGATTGTCAATAAACCTTGAAATGGTTCAAAAAATGATGAATATTTTAACCAACTAATCAAAGATATTAAACAAAATAATAAGAATTTAAAATTTGATTTTAACGTTTCATATATTTTAGACAATGTCGATATTGCCTCTGCTATTAAAGCTAAAAAAGCAAATATGGCTTTTATAACGTCAACTTTATTAAAAAATTCATTGCTAAAAAATGATGTTATTCCTTTACTGCAAACAAAAACAAGGGCTTTTTATTTTGACAAATTAGATGAACAATATGCTGATGGTTCTGACAATGATCCACTAAGAAAAATAGCAAAAATAGCAAATGACTTATTTAACAAAAAGCCTTATAAATCATGAACAGATAACGAATATCAATGAAATGGAAAAATTTATGAAAAATTTTATGATAAAGAAGGCGTCAATGTAAATTATCAACGCGGGTCAATCATGATTTTTGGAACCGATTCAGAAATTAAATCAATTAAAGAAGCATGAGAAAATAAAGACTGAAATGCATTTAGAAATTTTGGAATTGTAATCGGAAAAAAATCAAGTGGAACAAAATACATAGCACAAGAGGATTTATTTAAAAAACATTTCAATTTAGCAAATAATAAATTTTCTTCTTTTGCAAATGATCAAATAAATAATGCTAATAAATACATTACTGAAAAACCCAGAAACATAGGTAAGGGTGCACTGTCAAAATACCACATTGTTTTTGATGAATTTGCTTCTTTTGCATATACACACAATTGAAATAAATCAAAAAGCATAAACTATAATTTTTATTGCCCTGATGGGAATAAAAAGATTGAATTGCTAACAGTAACTGATCCAATAAAATTTAATGTCTTAGTTGTTGATAAATCAATACCAAAATCCGCTTATCTTGCTTTTGCAAATTCAATAATTAATTTAGCAAAAGAAAAAAAAGATACATACGGACCAAATGTTGGTTTCAATGAATATGAACTAATTAATGAATCTGACTATTCAAAATATTTTTAATTATTAGCAATTAAATTAAGTATATAATTTTTTTATAAAGAACAGGGGTGCCTTTGGCTGAGAAATACCCTCATTAGCTGATCTAGTTAATACTAGCGTAGTGAGTGTCTTAGTTTTTTGCATCCTCTTTTAGGAGGATTTTTTTATGCTAAAATTTAAAAAATATTTGCTTATAGGGGCAGCGGTATTGCCTACAATGGTTGGCGCATCAGTTTTATCTTCATCATGCGTTGATAAAATTTCAAACAATACTAAAACTTACGAAAATTTAAAATTTTCAATTAACAAGCCTTGAAGTGAAACAAGCAATAAAGATAATTTCTTTTATGAAGTTATTAATAAAATTAATGAAAATAAAAAAGGTTATAAAGTCAACAACGCAACGTTACATTATTTAGATGATAATGTTGATTTTATAAAAGAATTACAAAAAGGAACTGCTGATATCGCATGTTTAACACCAACGGTGTACAATCTAGAAAAAGATAAATCGAATATTCATCCATTTTTGCAAACAATGACAAGAGCGTTTGTTTTTGATCAAGATTTTAACGCTCAATATTCAAATGGTCTAGATGATGATCCACTAAGAAAAATAGCTCAATCAGCATTTGATTTATTTAACAAAAAACCTTTTGAAAATTGAGATGATAAGGAATATAAATGAAATGGTTCAATCTATGAATCATTCTATGCAAACAAAGACAAATTAGTTGATTATTATAGAGGATTGGTTGCTATTTGAGGAACACAAGAAGAACTTAAAGCAATAAAAGAAGCTTGGAATAAAAAAGATTGAAAAACATTTAGAAATTTTAGAATAATGCATGGAAGTAGTTCTAGTGGTTCAAAATACTTGCTTCAAGAAGCATTATTTAAAAAACATTTTAATTTAAAAGGAAATGAATTTAAATCATTTTTAGAAGATAAACAAGGAAACGAAACAAAATACATCAAAGGTAAGATTAAAAATATTGGAAAGGGTGCATTTTCTAAATACCATATTGTTTTTGATGACCTTGGATCTTTTGCATATACACATAGTAAAGGCAGCGCAATCTATAAATTGGATGACAAAAATTCAAAAATTGAATTTTTAACAGTTACCGAACCTTTAAAATATAACATTTTTGCCACATCATCAAATATTCCAAAAGAATTAAGGGAAATAATTGCAAATGCAATGGTCGAAGTTTGAAGGGAAGGCAAAGATACATATGGGCCTACTGTTGGATTCAATGGTTACAAAATAATAAATGATCCAATGAAGGAAGTTATATTGCCTTATAAAAATGTATTTAATATAAATTAGTTATGAATAATATTTCTTTTCCAAAATCTTTCTATTTTGAAAAAATAAAATACGATAATTTAACGTTAAGGGGACCAAAAAAAATTATTTGAAGAATTTTAGGTCTCGTTTTTGTTTTAGTCTTAATTGCTTTATCAATTTATTTTATTGACTTCTCGTTTGCGCCAAACGGAAGAAGGGTTTTTAGTCAAAATATAATTGACTTTTTTAGACCACATTTGATTAGCAAATTAATCCCTGGTCAAAATTTGTTTCTATTATCAATTAAATATTTATGAGAAAGCATTAAGGTTGCTTTTTTAGGAACGATTATTGGGACATGTTTAGCATTATTTACAGCATTTTTTTCTAACTACAAAATGAATAAATATTGAATAGCAATTCCTATAAAATTCATAATAATAGTTTTAAGATTACTACCAGAATTATTCTTTATTTATTTATTTCAAATTTCAGCAGATAAAACGTTGGCTATAAATTTGATTTTCTCTTGATTTACTTGGCTTTGATTACACGAATATTTTTCTCAAGTAATTGAAAATGCAAATTTTGAGATATTCTATTGACTAACAAAAATAAAACAAAATAGATTCACTGCATTCTTTAAAGAAATTTGGCCTCAAATATCTCCAAAAATTCTTAATTATATATTTTATGCTTTTGAATCAAACATTCGTTGATCTACAATTCTTTCAAAACTTGGTTTTTTAGGAATTGGAATATTGATCTACCCAAATTCAATCAATGTCAATTACTTTCATGAACTTCTAATTCCACTACTAATATTGGCAACATTTTTAATTTTTATTGAATTTTGTAACTTTATAATATCAAATTTGTTGTTTTATTCAAGGACTCAAAAAGTAAATATAAAAAAATATGCCAAAAATTCTACTATTAAAAAAATAATAATTTTTATAGCAATTGCAATAGGCTTAATAATAACTATCTTTGCATGTTTATCATTAAGGGGAGAAAAATTTTATTCAACCCAAGGAAATTACTATTTCAAAGAGTTATTTAATGGTGAATGAAAAAATGTTTTTCTCGGATGAAAAACAAACAGCAATTTCGGTCTTCTATATATGCTTTTGCAACTTGGGGCATTGGTATTTTTAAGCATTTTATTAACATATTTTATATCATATTTTAAACTGCTATTGCTTTCAAGACAATTATTCAAACGCTCATATTGAATTGAAAAAATTTGAAACACTATTTTAAGATCAATTCCTGTAATTTCATTATTTTTATTAATCTCTAATTTGTTTAATAAATTAGAAGCTGCTTTTGTTATAGCATTTGCAATTCATAGTTCTTCTACTATTTCTAGAATGTTGGAGAGCTCTATTAAAAAAATCTCGACAAACAAGATAAATGAACTAAAAATCAAAGGATATTCAAAATTTTATATCTTTAGAAATTTCATAATGCCAACAATTAAGCTAGATTTAATTACATTTATGACTTTTGAAATTGAAAAAATAACTAGAAACTTTATTACATATGGTTTATTTTCTGCTTCATTATTAGGATCTGAAACTAATCTAGGAAGATATAAAGAAATCTCTGATATTGCACCATATCTATGAATTGAATTTATTATTATTGGATTAATCAATCTAGGTAATTATTTATATAGATTGAGGCTAAATAAAGGCAGTATTAAAAAATAAAAGTTTTGTTTTAAATATCACTAGATAAATTTATCTCTAGTGATATTTTATTAATTAACTTAGATCTTTAAAAGAAATGTATTATTTAAAAATTATGTAATTTATATAATGTTTGTTATAATAAATATTAGACCTAGGGGTTTAGTATAACGGCAGTACCGCAGTCTCCAAAACTGCTTGTGAGGGTTCGATTCCTTTAACCCCTGCCATATAAAAAAAGTTTGTTAATAGCAAACTTTTTTTAAATTCAATATTTAAAAAATTTGCTTTGTTAGCAAATTTTTAATCTAATTTAATTTTGGTATTTTTTAGCAACATATTCTTTAATTTGATCAACACTATCAAGCTTTTCTCAACTTAAATTAAGGTCGTTTCGTCCAAAATGTCCATATGTTGCAGTTTGTAGATATATTGGCTTTAATAAATCTAATTTTTTTATAATTTGTTCAACGCTTAAATCAAATACATGTTCAATAATGTCAGCAATTTGTTCATCATTATATTGTGATGAATTAAATGAATTTATATAAATAGATTGAGGGCGTGGCAAGCCTATTGCATAAGACAATTGAATTTCAATCTTGTCTGCTATCTTAGCAGCAACTAAGTTTTTAGCAATATACCTTGCCATATAAGCAGCAGACCTATCTATTTTTGTAGCATCTTTCCCTGAAAATGCTCCTCCACCATGGTGAGCAAAACCGCCGTATGTATCAACAATTATTTTTCTTCCTGTAAGCCCAGTATCTCCAACAGGGCCACCAATTACAAATCTACCAGTTGGATTAATCAATAAATCATAATCCAAATTGAAATTAAATTCTTTTAACACATAATCAACAATATTTTTCTTTATAAAATCTTTAAATTCTTTTAAATTTGCATTTTCAGCATGTTGAATTGACATTAAAACTTTTTTAACAGTTATTTTATTGCTTTCATCATCATATTCCAATGTCACTTGGCTTTTCATATCACTTTTTGCATATGGAAATTGATTTTGTCTTCTTAAAAATTCAGCGCGTTTAAGAAGCTCATGTGCTAAAACTATTGGTCAAGGCATATAGAATTCATTTTCATTAGTTGCATATCCAAAAATAATCCCTTGATCACCAGCACCTAATTCATGGTCTTCTTCTTTATTGACACCCAAAGCAATATCGGCACTTTGTGGAGCAATACCATTAATTATATTAAAATCATTTTCATCATATCCTAATGGTTTCAATACCTTTCATGCTTCTTTAATTGTGTCAGAATATGCCTTTGTAGTTATTTGACCTCCTATATAAATGATGTTGTTGTTTGCTAAAACATCGCAAGCGACTCTTGCATATGGATCATTTGTCAACAAACTATCTAATATGGCGTCACTAATTTGGTCACATATTTTATCAGGGTGACCTGCCCCTACACTTTCTGATGTTAATAATTTTTTCATTTAAAAAACCTTTCTAACACAGAGAAAGGTTTTTATTATCTTTTTTTGTACATGTTAGATATAAATCCCTTACCATCCACCTTGCTTTATTTAAGTAGGTTGGCATGCCTTAATATGGTTAACTGGCAACTCTCTATGTATTTTTTGTTTTATTATTATACTAAAAGTTTAGTATATTATTAAAAATTAATAATTATAAAATTATAATGTTTTCTTTTGCTGATTCATCTAGGGTTTTTTGATCTCATGCAGATGCTTGAACTTCACCAATATGATGTTTTTCTAATAGAAACATTGCAATTCTACTTTGGCCTAGGCCACCACCAATCGTATATGGCAATTTGCCTTCTATTAAATCACAATGATATGGACTCAATTTTTTAATTTCTTCTTCGGTTTTATTTGATTGTTTAATTAAAGAATCTTTATCAACTCTAATTCCCATACTGCTAATTTCCAATGCTTTTTTATTTACTGAATCATAGACAATTAAATCCCCATTCAACATTCAATCATCATAGTCGAATGCTCTTTTTGAATGTGGAATTCCATCAGGTAAATTGTATCCTATTTGATAAATAAAAACAGAACCATATTCTTTGCAAATTGCTTCTTCGCGTTCTTCGGGACTTAGGTTTGGATAAAGATTATACAAATGGCTAGAACTAATAAAATAAATATTTTTAGCTAACTTTTGACTCAAAACAGGATAGATTCTATTAATAGATTTTTCAGTATTTTTTAATGCTCCATAAATTAACTTAACAATATGTTTTAAATATTTTAAATTTCTATCTTCTTTTTTTATAATTAACTCTCAGTCTCATTGGTCTACATATATTGAATGTTTATAATCTAATTTTTCTTGTTTTCTTATAGCATTCATATCTGTATATATTCCTTCATACAATCCAAATTCATATTTCTTTAAAGCATTTCTTTTTCATTTGGCAAGTGAATGAATAATTTGCAATTCATCTTCTACTTCGTCTGCAAAAAATACAACTGGTTTTTCTCCGTTCAAACCGTCATTTAAACCACTCTTGGTTTTTACAAACAATGGCGCTGAAACCCTGACCAAATTCAATGTTCTATGAAGTTGATGTGAAAACAGATCTTTTAATTTTTCAATAGCAAATTGGGTATCTTTCAATGATAATTTTGATGATGGCATATTTTATGCTCCTTTTTTAATTAATTTAATAAATTATAAATTAATATAACTCTTAAATATAAATAATTTTTTAATACAATAAAAATCAAGCCTAATGGCCTGATTTTTATTACTTATATTGCCAAATTTAAAACTATTTCTTTTCAACAGTTAGGCCGTATTTAGCACTTACTGATTTAATTAAAGCATCCATTGGCAATGCACTATTACGTAATAAAGCATCAAAGAATTCTTTTACATATTTATGATCATCTTTTTCAACAAATTCTCTTCTTGTTAATCCTAGTTTCTTTTGAACTTTTGTATATAGATCCATCATAACTGCCTTACCTGAGTTATATGAGGTTGCTTGACCAACATATGATAGGTAACGTCTTGATTCTGATGTTATGTCACCAATTCCTAGTGATGAATTCTTCTTCATGAATTCTCTTACTTGGTTGATTGAAGCACCTGCTGGCAAGTCTTCATGTCCTTTTACACCTTTACCATGGTAAGCAGTGTCTAATGCTAAACGCATGTTACGCAATTGAGCTTCGTTTAATGCACCTTGGTATTGCAACAAGTTAGCCAATTTTACAGCATCCATTGCATGTTTTTTGTCTTGGTTTTCATATTTATTAACTTGTGCAACTTTATTTCAATATACACCATTGTGTAAGTCTTTAATTTGTTGAATCATGTCATCTGTAACTTCTGCTTCTGTTTTAGCAGTAAAGAAGTTTGTTATACCATGTGCTGTTGAAAAGTCAACTGGCATTGCATATAAATCGCCGTCTTTATTGTCATAATCTGGTGTGCCGTAAAATCCAGCTTCAATTCCAAATCATTCCATAAATAGAGCTCAACCTTCAACGTATGAAGTGTAGTGGAATACATTTCCTAATTTGTATTTACCAAATTCTCCAACTGATGATAGATATCTTTCAGCATACATTAATTGGTTGTGGTGTCCCATCATTGATTCATGGTTTGCAAATGAAGTAACTGATCATTTTGGTAATGAGAAGTATGGATCGACATTAAACATGAATTTTTGAATGCCTGAGTTGTATGCTCCAACACCTGAAAATTCTCTTCTAGAAAAGTCATATGTTTGAATATCATAATCAGGGACAGGATTTGCAAAGAATTTACGTCCATAATCTTGAGTAGTCTTCTTAAATTGTTCATATGCCTTAAATGCCGACAATCCGCCATAATAGAATTCTTCGTTTGTTATCGAACCATGTTTTGTAGCTTTATTTTTTTCACTTAGTAATTGATTATAATTGAATTTTGTTAATTCATCAACATATTTCTTCAAATCAGGATTTGTTTTAAGACCGCTTTTTACAGTATCTGTTCAATAGGCACTACCTTCACGTCCAAAGAAGAATTCTTCATCATTTAATCATTTAGTAAATGAAGGAATATCAATGTTTCCTTTTTCATCACGAATTACAACAGTTTCTTCTTTTATTTCTTCTGGTCCAATACCATCAGCATCATATTTAATTTTGGCTTCTCATTTTTTGCCTTTTCCAACAATTAAATCAGCTGCTTGGTTAGCAATTTTCTTTGAGTTTTCAGCAGATTTCTTTGTTGTTTCATAACCAATATTATTTACTTGATCATCTGTTAAATCAGAAGTTGTAGCCATCTTTAGAATTTGACGATACATCGATTGACCATTTACGGTAGCATCTCCTTCAGCAAATCCTAAACCAACATTTCTTTGATTTAAGTCTTTATCAGTTAATCCTAAACCATATAATTTAACAATTTTGCCACTAGCATCTTCTGCATCAATAGACAATTCTTTTTCATTAACTTTATTTTCTTTATAAAGTTTTAATTCTTTAATGTCTTCACCTAATCCATGAGTTGCAGCATTGTAATATGTAGTTGCATAATATTTTAATAATTCTTTTTTGTCTGTTGGCAAGCTAGAAGAATTAATTAATTCTACAACCGAAATTTCATTTTTAGAATTGTCATTTACAAATTTAGTTAGTTGTGTTTGAAATAATCTTCCCACAAAAGCATTGATTACCATTTTTGAGAAAACTTTTGACATAACAACTTTATGTTGCACTGCTTCTTTGAAGTTTTCTAATAGTTGTTTAAATGGTAGCGAGCTTCTACTTCCAAGCACATTTGTAAATGCATTCATTGGGTATACAGATGCATTTCCTCAATAAAATGTAGGGTTTAACAATCTTAGTTGATTGTCATGGTGTCCTTTAGCGATTTCTCAATCGTATTTTACGGCATATTTTCATGCTAAAGAATCACTTGTTAGTGAAGCGTCATTTATTTTTGAAATTTTTTCAATATATTTACCATAAATTCCAGAAATTTTCTTAGATTGTTCAGCAGTAGGAGCAATTATTCCATTTAATTTTTCTTTCTTTAGTTCAGATTCAAGATCACCTTTTAAATCTAAAATATGTCTTGCGGCTGCTTCTTGGCTAGCTGGTGAATTATCATTTTCTTTTGAAAGTAAATTGCTTAATTCATCGCTCTTTTTAACGCTATCATTATATTCTTTGTCTAGCGCTTCTAATTCCTTTTGATTAGCTTCAATTTCTTTAGAAATATTTTCTTTTTTTGCTTTTAAATCTGCAACTTGAATTTCAAGTTCTGACTTTTTATTAGTTTCTTCAATGATTTTATCTTTTTGAGTTTTTAATTCTGTTTGAAGACCTTCAAACTTTTCTTCTTCTTGCTTAATTTCTAAATTTTTAGATTTAATTTCTTCTTTTTTTGATGATATTTCATTATTTTTTGTGCCATCAGAATTTTGTTTTTCTAATTCTTCTAAATTTTTGTTTAATTCTTCCAAAGTTTTTCTTAGGTCAACTAATTTAGTTTTAGATTCTTTAATTTTGTTTTCTTTTTCTGTTGATGTTTTTAAATAGCCTTCTATTTTTCTTCCTGAACTTTTAAACTTGCTTTCAGCAGTCATTAGCTTCATTTTTAAGCTGCTTAATTGATCTTTAAGATCTTCATTTTTTTGTTCTAATTCTTCATATTTCTTAGGAATTTCAACAATAGCCTTGTTTGTAGAATTTAATTCTGCTTGATATTTAGCTGTTCTTTCTTGTTGGATTTTATTTAAATCTTCTTGCAATTTTTTAACTAATTTTTCTTTTTCTTCAATTGTTTTTGAATTATTTTTTGTTTCTTGCAATTGCTTCATAAGATCATTAATTTGTGCTTGATCTTTTTGAGAATTTGCTTTTGCTTCATTTAATTGCTTTTCTAAAGATTCAATTTTTTTGTTTAATTCATCATTATTTGTATGTGTACATGATGCTGCCATAGGCATTAAGGCTGCTACGCCAAAAATTGAACCTAATGAAATTAGAATTCTAGTTTGTTTTTTCATTTTTCCCTTCTTTAACTTTTAACTTATATTGTTCTTTTTTACAACAAAGTTTTTATTTTTATATAGTCATAATTTTAGAAAAAAAAAAAAAAAACGCTTATTTTGTAATTTTTTTGCATATAAAATACAAAAAAAATTAACATTTTCTTATAGTTTTAAGTACAAAAAAGCATAATAAAAGCACAATAAAAAATTTTTTGCTAACTTTAAATAGTGGGATTTATGCTTTATTTTTGTAAACTATATATTTTTTAAAAAAGTGGATAAAAATCAAAAATGATGTTGCTTTTTTAAAAAAACCTAACAAAATAAAAAAATTTTAAAAATTTTGAAACATATTTAAAAATAGTATATAATAATACACGTAGCAACCATAACGAAGGGGTCCACCTGAAACCATTCCGAACTCAGCAGTTAAGCCCTTCAGTGCCGACGATACCTCAATGGGAAAATAGGACGTTGCTTTTTTTTATATCTTTTTTTAATAAAAAATAAGGCTTTTAAACCTTATTTATATATAGATTAATATAAGTTGCGATTAACAATTATTATTTTTAAAAATTTTTTTACCTAATTTAATATACTTATCAACTTTTTGATTCAAAACCTCAAATGCTAAATTATAAATATCTTCATTATAAATATCAACACCAGCATCTTTTAAAATATTTGCAGGTCAATCGATGTCTCCGGCACTTAAAAATTTATTTATATAGTTTTTTAATGCTTCTTGGCCTTCTTGTTTGTATTTTTGGAAAAATACATTTGCTACGATATATCCTAATGCATATTTATATACATAGAAATAATAATAGAAATGGGGAACCATTACGCTATAAACATTTCTAGGATCGCCTAGTTTTATTGATTTTGATGTAGTTGAGTATTTCTTTTCATTTTCAACATATAATTTTTCTATTTCTTCATATGTATTTAATGGTTTATTTTCATCTATTGCATTATATAGATCAAATTCAAAATTAGATCACATTGTTTGGCGCAAAACTGTGCCAATAAAATCATTAATACTTGAATCAAGTAAATAAAATTGTTGTTGAATATTTTTTGATTTGCTTATTAAATAATCATTTAGTAATAATTCATTAAATATCGAAGCAATTTCTGCTAAAAATATTGGATAAGAGCTTCTAAGTGGAGATTGGCATTTATTAGAAAAATATGAATGCATTGAATGTCCCATTTCGTGGCATAATGTATTGACTGATTCCAATGTTCCATCAAAATTCATCAATATATAAATTTTTTTCATTCCTGCTGCCCCACCTATAGAATAAGCGCCGCTTCGTTTAGATGGGACATTAATATAATCAACCCAACGTTCATCAATAGCTTTTTTAACAACTTCTGGATATTCATAAGGCATTATTGAAATAACATCTAATAAAATCTTTTGTGCTTCTTCTATTGTATATGAAGATTTAACATTAACTAAATCTAAAAAGTAATCTCATTTTTCTAATTTTTTATTGAATTTAGCTTTAAAAAATTGTTTTTTGGCATTGTTATATTTTTTAAATATATACATATGTTTTTGAACTGCATCATAAATAATGTTCAATAACTTTTTATCAACATGATCTTGCAACAAAATAGATTCAAGAGATGAATTATATTTTCTATATAAAGCATTAACTGATATTTCTTTAATATGTTGATATAGCAATCTCGCTAATGATTGTTTGTTTTTTAAATATCCATTTAAATAATTAAAATAAACATCTTTTCTAACTTGTTCATCTTTATTTTTTAATAAAGCTATTCTATTTCCTTCAGTTATTTTTATTTTTTTATTTTTAATTAAAACATTGCCAAAATCAACTTCACTATCGCTTATTATTGAAAAAATATCTTCAGGTACAGGTGAACCGCTTGCAGTATCATTTAAATAATTTTCAACATCATCTGACAATTTATGATCAAGTTCTTGCAAAGTTGCTTCTAGGTCTTTTTTTACTTCAATTAGTCTGTCGTCATTAATTCATGTTTTGATTTTGTCTTTATGTTGAAAAATTCTGTTGGCTTCTGAACCTAACTTTTTAGCATATTCTTGGGATTTTGTTTCAAATAATGAAATTGTTTTGTTTATTGTGGGATCAACAATATTTATATTTAATTTATTATCCAAATAATTAGAAATTCTATTGCCTAAAATTAGGAATTTTTTTGAAAGTTCAATATATTCAACATATTGGTCTAATGAATTGTATTTGCTATCTTTTATTTCAATTATTTTTGAATACAAATCAAAATATTCATCTTCTAATTGTTGGTAGGTTTTATTACCTAATATGTCTTCAATATCTCATTTATATTTTTCTTCAACGTCACTATATGAATTGTATTTTTTTAACATTATTCCCCTTTATTAATATAGATTAAACTTTTTATATCGTATGGCAATCTTTCATGAGAATTAAAGCCATCTAATTCCATTAAAACGATTATTCTACTGATAATTACACCTTGTTCTGTTAATAATTTAGTAATTGCTTCCAATGTACCCCCGGTTGCTAAAACATCGTCAACTATGACGGCTTTTTGACCTGGTTTAACTAAACCTTTTTGAATTTCTAAAATATTTCTACCATATTCAAGTCCATAATCGCAGCTTATAACTTCGCCGGGCAATTTGCCTTTTTTTCTTACCATAATAAATGGTTTTGACAATTTAGCAGCTACTGGTGTTCCAAATAAAAATCCTCTTGCATCAGGACCCACAATAATATCTGCACCTTCTGCTAATTTTGCCATTTCATCAATTGTATAATGTAGCGCTTGTCCATTGGCTAATAAAAGCGAAATATCTTTAAATTTGACACCTTTTTGTGGAAAGTCATCAACAGTTCTTATATACTTTTTTAAATCCATAAATTTCTCCTAACAATAAGTTGATAAATAACGAATAAATTTTAATATTATTTACAATTTTATTTTATAAAAAAATGATTTTATTTCAAACAATTGAATAAAAAACTAGTTAAAATAAATATATTAATTTGCTAATTTTTTTTTATTTATTGATAAAATAAAATTAACTTAAATTCTTTTATTTTAAATTTTAATAATTACTCGGAGGATAGAATGAATAAAATTAATTTTTTTGCATTAGGTGGTTTGGATGAAAACGGCAAAAATTGTTATGTTTTAGAAATAAATAACAAACTATTTGTAATTAACTCAGGAACTAAAATTCCTATTAACTCGCATAACGGTATTGATACATTAATTTGCAATTACGAATTTTTAGAAAAACGTCAAAAAGATATAGAAGGATTATTTTTGACTGATGTTAGAAATGAAACGTTTTCTGCTATTCCTTGATTATTAATGAAAATTAAAAACCTAAAAATATATACTAGTGCTTTTAACAAAATTGTTTTATTAGATAGAATTTCTAAATATAATATAGGTTCGAATACATTTGAAGTTAAAACAATAAATAAAAAAACAGCATTTGGCGGAGTAAATGTAACTCCTTTTGATCTAGCCGGAGGATTGCCTGGACAACTTGGGTTCAATTTCGAAACCCCAATTAGCAATATCTTGTTTATGACAAATTTTGTTGATGGTGATTTGGGTCCTTATGGTAAAACCGATTTGCAACAAATTCAACAAATAATTAATAACAAAAAGGAATTACAACTTTTAATAATGGATAGTTCAAGAAGCGCTTTTCCTGGACGTTCAATTGAAAAACTTTGAGTTTCAAGAAAAATTGAATACAAATTTAAAGAAACATCAGATAACCAAAGAATTATTGTTGGTTTATATGATGAAGATATGATAACTGCACATGAAATTTTAATACTTGCAAAACGTTATAACAGACCGGTTATAACTTATGGTTTAGCTTATTCTCAATTAATAAAACTTGTTGAAAAGGTAAATCCAAATTCTCAATTTCCGGAATTCGTTGAATATCAAACTGCAAATAATGTAAAAAATGCTGTTATTCTTGTAACAGGTGCAATAGATAGACTTTACACAAGATTTGCAAGAATTTCTAGCGATAATGATGTTTATTTAAAATTGAAAAATGATGATGCAGTCATTATAATTGCTCCGCCAGTTAACGGATTAGAAGTAAATTATGCAATAACACTAGATGATATTGCTAGACATACTGCTAATTTAATTGAACTAGGCAATGATCAATATTATTCATGCAATCCTGCTAAATTAGATATTTTTAATATTATTAAAGCTTTAAAACCTAAATTCTTTATACCAATTCAAGGTCTATACCGTTACCAAGTTGTAGCAACTCAAGTTGCTAGAAGCGCAGGTATGAACATTAGTAACTGCTTAGTGTTGCAAAACGGAAAGGTGGCCGAATTCAACGATACAAATTTAATTTCTCAAAAACATGGAATTAGAAATGTAGGTGATGTTATTATTGATGGCTATGGTATTGGCGATATTTCTCATGAAGTTATTAATGAAAGAGAAAACTTAGCAAGAGATGGTGTAATTGCTCTTAGCTCATTAATAGATTATAAAAAGAAGAAACCAATCAATGAACTTCAAATTTCTTCATATGGAATAATTACGAAAGATAATAAAGAAGTTATTCACAAAATAATTAATGATTTATTCAAAAAAGAATTTGAAAATAAACAAACAAAAGAAATAAATTTAAAGGAAATTCAAGAAAAATTAAGAAAATCAATTAAAAGAAAAATCGCCAAAACTATCGACAAAGAGCCAATGATTGTAATAACTTTATATGAAATTTAACTTTTAAAAATTATAATTATAGTGATAACTAGGAATATTTATGAAAGTGGCAAAGGAAAAACAAACTAAAATTAAGAACCAAAAACAATACTTTTTTTCAAGAGATAGGGTGCTTTTATGAACAATAATACTTTTAGTAACATTGTTTTTTTTAGTTATATCTTTCCTTAATGTTCCTTGATTAACAACATTGCATTCATATTCATTTAATGTATTGTTTGGAATGTTCTCAATATTATTTTATTTATTAATTATTCTACTTTCAATATGAAAAATCTTTAATTTAAAATCAACATATAAATATTGAATATTTAATTTTAACCTTGGAAGATTAATATTCTTTTTTATTAATATTATAATTTTGGGTTCTGTTATTTATTACACTATTCATCAAGTTGAACATAATTCAAAGAATTTTTTTGCTAAATATTTTAGCAGTTGATTTGTTGATTTTAGTAATAGTTCAAACTATTTACTACCTCAAAAATACACCCCCGGAGTGATTGGAACATTTATTAATTCAATATTAAATATTCCTGGAAAAAAACTCGGTTTTTCAATAGCTTACATAATAAGTATTGTTCTTTTAAGCTTATCTGTCATTTCATTTTTTATTAGTAACTCTTTCTTTAAAATGATATTCTCTCCAAAAAGCAAAAAGAACAAGGAAAAACTTCAAAATCAAAGCGATTTAAAAATAAAAGAAGAGGATATTGAAATAATCGATAACAAAGAAGTCGTAAATAGCAATATTCAAATTTTAGAGGCTAATTCTAACTCAAATAAAAATATAGACTCAGCACAAAATTCAAACCAAAATAACAATCTTGATTTAGATCCTTTTGATGATGAAAAGGAAATTGAAGAAGATTCAAAGCAAAAAGACAACAATGAGTTAGAGCCAAAAGAAGCTATTGACTCACCAAGCAATGAAAATTCTATAGATCCAACCCCGCAAAACCTAGATAATTCAAATAAAAATAAAGAGATTAAAAAAGAAGATAAGATCTATAAAATCATTGAAGATGAAGAAGATTTATTCTAATTAAAAAAATTCATTTTTTGCAAACATTTATTTATAAGAACAAATTTCAATAACAAAATAATTTAGGCGTTTTAGCTATAAACAAAGAGCATTTAAATTGCTCTTGTTTTTTTTATATACAAATTATAAATTTACATAAATATTTTTTTAAAGGTTATTTTTATATATAATTTTTAAGTAAAAAATTTATGATTTAGTTTGCTAAAAAAAGCAAAGTATAACTGATAGACTTCATATTTAACAAATAATAGTTTATTAATTTAAAATAGTTTTTTATTTTTTAGATGTATTGGTTCAATATATCTTAGTTAACTTTTAAATATACACACCCAAGCACAATAAATAATATAGAAAGGTGCTAGTAATGAAAAACAAAAAAAGTAAAAAAATTGTGGTTGCACTTTCATTAACATTGATTGCATCCGCCATCACAATACCCGTTGTAATTTCATCTTTAATTATAAATAAGAATAATTGGGCTAACAAATACTATAGCCAAAGATCAGAGGCTAGTCATCTAGTTAATTTAATAGAAAAAAATAAGCATTTTTATTATTTTGCCTTAGAGGATCAAAATAATTTAAATAAGTTATTGAATAAAATTAATTTAACCAGCAAAAATAGTTCTGCTGATTTTAAAAACTCATATAATTTGCTTAACAAGACAAATCTTGATATCAACAAAAAGATAAATATTAACAAAAAAGAATTTGATAAAACTTATGCTGATATTTTGCTTTTAAGCTCACAGTTGCAAGATTTATTCAATAAAATTATTGATTTAAAATTGAACAACAATATAAAAGAAGATGTTGATAGCTTATTACAAAGAGTTGAAAAAGATCTAAATAAATGTAGAAAAATAGATGAAAACATTAATCTTATTGATATTAAAGAAATATACGCACATAGTTCAAAAACAATCATTGATGTTTCAAATCAATTGAATTCATATTATAAATTAATTAGCAATAAAGATAAAACATATAAAGGCATTGTCTCAGAAATAAAAGATCTTAAAAAAGATTATTTCAACAAAATTGAAGATGGAGAAGAATATTACAAATTATATAGCAATGAAATAACCGATCTTAAAAAGAATTTTAAAAATTTCCAATCAGTTGAAGATTACAACAATTATATAAATTACGCCAATTCAAAAATAAAAGAAATTAAAGAAGAAATTGTTGAACACAGAAAGCTAAGCCAAAAGATAAAAAAAGATGGAAATGATATTCTAAATAATGATTATGATAAAAGTGCTTTAATAAGAAATTCATACTTCCAAGAACCGAAATCATTTTTATTAATGTGAAATACCTTCACTAAATTAGATACGTTAATAAAGGCAAAATTTATTAATGAACGTCCTAAACAAATAAAAGAATTCCTAAAGCAATATGAAGAAAGGCAAGAATACTTTTATAGAATTAATGAAAACGTTCCAAACCTTATTTACACATGAGTAATTTATAATAACAAGATTAGAATTATAAAAGAATTCTATTTTAAAGATAAAAAAGAAAACGAAGAATTTAATTCATTTTATTACAAGGATAAAGATATAGACAATCAATTAAATTCGCTTCTTAATGAAGAACGAGAATTTAAAAATAGGCAAGAAAGACTAGTAAGATTGTTTTATGACAAGGTTATTCAAGACCCAAAAACTAACGAAATTAATGAAGAAAAATTAATGAAATTAGATGACATCCATTTTGACCCGGAACAAGATATTAGATATTTACACTACCATTTTGGAAGTCTCATAACTCAAAAGGTTAATGAAATAATTAAGCATTATAACGAAAAAGCAAAAGCCTTATTAGAAAAAATAAAAAACAATAATAGTTACGAAACACAAAAAAATAGAATAGAAAAATTACTTGCTAAAAATATTACTTATGTAAATAATTTTAGAGGCGATTTCAATGATTACGACAAATACTATTGGTGAACATATAAATTTCAAAAAGACAACAATGGTGATATAATCCTTTCGCCAAATAGCAAAATCCCTGATTTTTATAAAAATGAAATTGAAGTAGCCCAATACCTACATGAACTTGATGAAATACTTGATAATTTAAAAAACATTTAAATATTTGTTTCTTTAATAAAATGGCATTATATGTGCCGTTTTATTTTTTAATAATTAAGTCAAAAAATAAATTAGATTTTTATAATGCTTAAAATAAGTTACTTTTTAATTAAAACTTAATTTGCTTTTGCAATAAAAAAACTAAAAATAATAAAAAAATTTTACATATTTGTTAAAATTAAAATGTTTTTTTACAACGAATTAAAAAAATAATACTTTTAGAAAAAATTAAGGAGAATATTATGGAAATGAGAAAGAAATTAATTGCTTTATCTGTGGCTTTTGGACTTATAGCGCCTATTTCAGGAGCTCTTATGATTTCATGCAAAAACGAAAAAAGTAATGCTGAATATCAAAAAGAACTGCAAAATAAATGAACAGAACTTAAGAATCTTTTAAATTCAAATTCATTTAAAGAAGTTGAATCTAAGTTAGATCTTTCAAAATTAAACGCTCTTAAAGAAGAATATAAAACAATAAACAGCAAAAGCAATGTTTCAGAATTGAAGAATTTTATTGCAAAAATTAATGAAACTACACAAGATATAAATGCACAAATTTCAAAATTAAAAAACGAAAACGGAAATACAAACTCTGAAAACAATAGCCAAAATGATTTATTAAAAAAATTAAAAGTAGAATTAGAAATAGCAACTACAAAACTTAACATGCTACTTTCAGCAAATGAAAGTAAAAACATCTCAATAAAAGAAGAAGCTAAAAACATATTAAACGCCGCAAATAAAGCGTTAGAAAAATCAAATGAACAAGAATATAAAAATCAAATTCAAAAAGTAAATGAAACCATCAATAAAATTGAAGAAGAAATAGCAAATTTAGAAAAAAGTCCAAAATCAAAAGAATTTACTAAATTAAAAGAATTAAGGGAAAAACTAACAAAATACTTAGATAATAATTTCTCTGACTTTGCTTATAAAGATTTAAGAGCTGAAATTAAAGAATTAATTCAAAAACAAGAAAACGTTTCAATTGTTTCAAATGAAAAAGAAATAAAGTCAGCAATTTCACAAATAAATGATGCCTTTTGAATTGCAAACATCAAAAAATCAATACTAGATAAACAATTTCTTTATTTAGAAAATAAACCTAAATCTGAACTAGACGTAAAATTTTTAGCAGCGTATGATGAATTAAGTCAAGCATTGAATACCGCTATTTTTGAAAACATTGAAACAACAAATGAAAGAAATCTATTAAACAAATATATTAAAACAAATTTAACCAAAATTTCTAATGTAGAAAAACAAAATTATATTGAAGAAGTTAAAAATACTATAAACTCTTTGTTAAACAAATATAATAATAATATAAATAAAAATAAAGATCAAATTTATGAATTAAGTGGAGACATAGAACAATTAAATAAATTAAATCAAATAGAAAATAATTATGTTATTGACAAAGTCAAACTACTAAATCTATTAAACAAAGCAAATACTATAAAAAATGAAGGTTCATTTGCTAAAAAAGCAATTTTACTAGAAACATTAAGAGATACCATTTCGGAATTTCTTGAATTTTATTTACAATTAACCCATTATCTAAGAATCTTTTTTGACAAAAATAACGACATTATTAAATTATTACCAGCAGATTCACAAACAAAAATTAATGATTATCTAAACAAAATTAAAAAAATTACAGAAAAAAATAGTCAAACCAGAGGAAGCGAACCAACATTTGATGAATATATTTCAGCCTTTAAGGCACAAGAAGAAATTTTTAAAATTATAAAATCTAGTGATCTAAAATCAGCAGATGTTAACATATTAGAAAAATTGACAAATAATTTAAAAGAAATTAAAAAAGAAAAGAAAAATGATTACAACTTGGTTGTTACTTCTTATTTAAAAGAAAAAATAGAAGAAGCAATAACAAAAAATGAATCAATAATTGCTAAAAAATCTAATATTGAAAGTCAAAATATTATTGAAACGTTTGCTTTATTGCAAAAATTAGAACTTGAATTTGCTTATATTAATTGAGCTGAATTAAATTTGATTGCTAAAATTAAAAATAAATTAGAACTATTAATAAAAATCAATAAAATAGATTCAAATGAAGAAGCAAAAATATTAAAACCTTGAACAGATTTGCTTAATAGTCCAAAAAAACCAGAAATTTTCGAATTAGAAAACATCTTAAAAGAATTAAGCAAATCTTTAAATGAACTAATTAACAAATAAAAGTTATTTATTTAATAAAAACATTGTAAGAATTTTTTTGTAATGTTTAACCTACTTTCTCAACAGAAAGAAATTTACATTACACAACCTATAAAAATAGTGTATAAAAAACAAGATAAAAGTTCTTGAACAATTATCTTGTTTTTTATATTATTAATTTTGTTGCTATAAATATGCAATTTATTGGGCTTTAATTGTTATTTCGTCGATGGTTATAGGATTTGCCGTTCCAATATATTTATAATAAGGAATTTCTGGATCATAAAATTGAATAAATCCTTCAAATCTTAATTTTCCATCTTGTTCATAAATTCCTTTAGTTTTTGGTCCCCCTAAAAATGTATTAAAATTCACGCGTACTTTGCTAAATAATTTAAATAAAGGAAGACTTCTGTCAAAATTTAATTTTCTTTCATCACGAATAGTTTTATCAGGCAAGAATGTTTTTATTTTATAAACACTAGCTTGACTAGTTTTTTCAAACATATCATTAATCATTTTATTAAAAATTGCTTGTTTAACTTTTGCAAGTGAATTGTCAAAAACTTTCTTTTTTTCAGCAATAAATTTGACATTATCTTCATTCCTTACATAATAATCTCAATTTAAATTTTCGATATTGCTTCTACTTAATTCTGTTGGATCTGAAACATATTTTTCCATTATCTCTTGTTTTATTTCATCACTTAATTTTGCATCTTCTGATTCAATATATTTGAATACTTCTTTTAAATAATTAGCGTACATATCAACTATCTTATAAATATTTTTAAGTTGGTTTATAAATTTCTTGCAATCTTCTTTATTTCTTTCATTATATGCTCTTCTATAATAATATTTTGTTTCATAGCCGGGATAATCGCCATAAACTTCATTAACTCTTTGACTAATTCTCTTCTTTATAGATTCATTTGATTCATCAACTAATTGCCCACTGTATGTTTTATTGAATTTTAAAGAGCAAATTTCATTTAGTTTAGATTCAATTTGCTTCTTTTGGTCTTGAAATTCTTTTTCAATTTCTAAAAATTCATCACTTGGTGGCACAACTTCATGGCTTTTAGCTTCATTAATTTTTTGAGTTAATGATTTGATTGCATCTTCAATAATCTTTGTTTTTGATTTAATGTTTGAAATATTTGATGATTCGGCAGCATTTGTAGTATTTAATACATTTTGTTCTTCGGAATTATCAACATTTTTTGTCTTTGCAGAATCAATTAATGTTTGTAATTCTTGTTTCTTAGTTTCAAAATTTTGTTTTACTTGAAGTTTGGTTGTATTTATTTTATTTTCTAATTGTTCTTTTGCTTTATTAAGATTGTTTGAAACATCTTGTATTTTTGAAATAGTTCAATCTTCGGCAATGTTTGCTTCATTCAAGGTTTTGTTTATATCATCAATGTTTTCTACTTCTTTTGCATCAGGGGTTGCAATTAATTCTTGTAGTTCTCTTTTTATTTTTTCAAAATCTTGTTTTGCTTGTTCAAGTTCTTGTTTATTTTTTTGTTCATTAATTTTTTTATTCAATGATTTAATTGCTTTTGAAATTTCATTAGTCTTATTTTTAATGTCTTTAACTAAATCATTATCTTTAACTTCAATATTCTTATATTTTTCTTCTTCGTCTTGTTTATTAATCTTATTGTCTATTGAATCTAGAAGTACTCTCAATTCTTGTTTTTTAATATCAAACTCATTTTTTGCTTTATTTTTGGTATCTTCTATTAAATTTTGAAGATCTTGTTTAGCTTTAATAATTTTGTTTGTTTTTTCTTTAACCTCACTAATTGAAGAATTTTCTTCAATGTTTGTTGAATCTAAAATTTCTTTTGCTTTTGAATTTTCAATTCCTTGGTCAGCGCTAAAATCAATAAGATTTTGTAATTCTTGCTTAGACTTTTTCAACTGTTCAATAGCAATGTTAAGTTCCTTATTTTCGTCTTTCGAAGGCGAATTTTGTTTCTTTTGTTCGTTAATTTTTTGGGTTAAAACATTGATTTTTTCTTCAATGTTTTTAATAATTTCATTATCTCCTTGACTATTATCCCCTTTAAATTCTTTTTCTTGGTTAACTAGTCATTCATTAAGTTCTTGTTGTTCTTCATCACTTAGTTTTGAAATTAAATTTTGAAGTTGTGATTTTAGAGCATTAAACTTTTCTTTAACTTTTACAGTTACGGTACATTTAGATGCTAAAACAGGCACAGAGCCCGCTGCTACACACAACGCTAAAATTTGTAATGATAAATTTTTTTTCTTCATTTTAGTTATACCTTTCGTATTTATTTAAGCTTAGATATATAAAATTATATATATTCATATATATAAAAATATAAAAATATTTTTAAACATTGCAAAAAATATTTTTATTCATATTAAAGTTAAAATCAATATTTTTCGCTAATTTTTATTCTTATATTGAGTTTTAATATGATTATGAGATAATTTTAAAGTAGTTAAGAGGAAAGGGAGCATATGGAAGATCAAAAAGATTTTAATATTATAGATAGTGAAATAGCAGCATTTGGCAATGTAAAAAAGATCTTTGATGAAAATATAAACAATATAAATGATATTTGTAAAGAAATAATTAAACAGGAATTTTTAGAAAAAAATTCAAATAACAAAAGAATTGAAGAAGCTAAAAAACAATACGAAATAGTAAGAGATTCTAATGAAAGAATTATTGCTGAAAAAAAGCTAAAAAAAATATCAGGAAGGAAATTTGCAAATTGCATAATTTTTATACTTTTCTTTATTCTAATAGGTTTTTGTTTTTTTTCATTTTTTGCTAACAATAAGAAAATAATTAAATCATTTGAAGAGTTTAGAAATAATGAACAATTAAAAATTGATGAAGCTTTAGGCAAGGCAAGACAGGCATACGTTGAAACCTTTTCATTACAAACCCTTGAAGATATAAAAAATAAAATTTTAAGCAAAATGGGGATAAAAAAAATATCACAATACGATGCTAAAGTTGGTGATTTTTCGCGTAGTTTTATTTATGATAAAAATTTTTGAAATTTTAATAGCATAAGTCAATATAAATTAAGAAATTCTTATATTTATGACATTTTTTATACTTGCAAATGAATGAAAAATGTTGTTACTAAGGCTTCAATTACCGTTACTAAAAAAGATGCTAATGGTAATACTTATTGAGAAACATATTGAGCATATCATACCGAACCTACACCTGTTGTAGAATATTTTAGAAGTTTAACCATACCTTCAAATTACAAGCAAAAACTATACTTTGAAATTATAGAAAATAATCGAAGTGAAAAAGAAGTAAAAAGACTTTTAAAAAATGAAGGCTTCCACCTTGAAAATAAAGAAGTCTATCAAAATTTTGATTTTGCATTTTCTGATGACCAAAAGATTGATTTTTTAACTTATTTTGATGTTAGAACTCAACAAAACCTAATCAATTATGCCCAATATATGAATCAAGAAAACATGCCGGTATTTTCAATGATAAAAAATGGAAATTGGTTGGTTTCTAAAAATACGATTCAATCAAGTTCCTTATTATTTGAACAAGTTCAGGATTGGATGTTAAGTGTTTTGGATTTAACTGAAGAACTAACAGTTGACTTTGTCACGGAATTTTTAAATTCAAAAATTATTGAATGTTTATTGCCAATATTTAATTTTATTTCCTTAGTATATACAAATAAGTATATTGCAACTGAAAATTTAGAAAAAACAGAAAATAGATATGAACAAGACCTTGTTGTTAAACAAAATAACAATATTCAAAATTGCCCCCTAGACATTCACTCATATTATAGAGACATGACATTATATAGTGGGTTTTATAAATTTAACAAGCATGCAGATATAACACCAATTTGCGAATTTAAAAATCTTTATAATGACCCAGAATCTTTGATAATATCGGCTACATTTGATTTGATTTCATATACAGCTAGAGACGCAATTGCAATTGAATATACACACGGTTACGCTGTTGAAGTACCATATGTGGCATATGATGAATTTAGAGAACATAAAGCAATAATTGTTGTTCCTTATAATATAAAAACAGAAAATGCCAAAATATTTGCAAATAGTCTTATTGCTAATAATATTTATGTAAGCGACGATCGCATAGATGAAGAATATAGCAAAATTATTCAAAAAAATAAAATTTCATTTAATTTAGAATCATTAAAGGACTTTGATGAATTTAAGAAAAATATTAAGCTTATAGATGAATTATTTAGTCAGATTCCTGAAATAAAAAAGATTCCAATACTACTTGATAATAGAGGAATGGCTTTCTTTATAAATGAGCCAAAATTATTTGACATCAATTTATTAGTTAAAAAGCTTAATAATTTTTATTAAAATTAATTAATCTCTATTTTTGTAATTAAAATAAAAATAGATTACATTATAATTTAATTACTAATAATAAGCAATAAAGGAGCAATATGTTATTTGATTCAAGAACCCCACAATCTAGTGAAGGATTTAAACCAAACGTAGATAATAGCATTAAAAAGCCAATTCCAACAGGTGTTGAAAAATTCTTTTTTATCTTATTTTTTATACTTACAATAGGAATTTTCTATTTTGTATATGTAGGAAGAAAAAATGAATTAATGCGTGATCAAAACGAAATTCAAAATGCAAGTTCTCTAATACAAGCAGCAGAAAAAAGGAGACGTGCAGTTCTAATTAAAATGATGGATAGTTTAATTGGCTATAAAAATTTTGAAAATGAAACATTAACTAAAATAACTCAATATAGATCAAAATTATCAAATATTGATGTAGACAAAACTTCTCCCGTTGAATTGAAATCACAAATTGACAGCATAAGAGGTGCTTTAAACTTTCAATTTGAACAATACCCAGATCTTAAAGCAAGCAAATTGTATTTACAATTTTCAACTGAAATTTCTATGCAAGAAGATGAAATTTATGCAACAATAAGAAACTATAATATGATTGCAACATCATTTAATTCAAAAATATATACATTTTGAACAAATTGTGTTGCTCAAAAATTAGATTTGTACAATGTGGCTATTTTTCAAGCATCCGAAATAGAAAGAGCAGATGTAGATACAAGCGAACTTAGAAACTAAATGAGCCGAAAGGCTTATTTTTTTGCTTTATTTTGCTATAATATAATATATTAATTAATTAATATATTACAACATGCGAGGAAACATGGACAAAATAGAAGAAATACATAAATATAATGCCGATAATATTCAAATATTAGAAGGTTTAGAAGCCGTAAGAAAAAGACCCGGCATGTACATAGGCTCAATAGGATTTAAGGGTTTACACCATTTGCTATGAGAAATAGTAGATAACTCAGTCGATGAAGCAATGGCGGGTTTTGCTACTGAAATTAAAATTAAATTGTATCCAAACAATGTAATAGAAGTTGAAGACAATGGTCGTGGAATGCCCACTGGAATTCACTCTGGAACTAAAAAGTCAGCTGTCGAAACAATTTTAACCGTGCTACATGCTGGTGGTAAATTTGATGGATCAAATTATAAAGTTTCCGGAGGGTTGCACGGTGTTGGCGCATCAGTTGTTAATGCATTAAGTAGTGAATTTGAAGTATGAGTTAAAAGAGATGGCAAGTTACACTACCAACAATTTAGAAATGGTGGAATTCCTGTTAAACCTTTAGAAGTAATTGGAAATGTTTCTGAAGTCGAAACAGGAACAACAATTAAATTTCACCCTGACTACACCATAATGGAAAAAGAAAATTTTGACTTTGATACAATTATTGACCACTCCAAACAAATTGCTTATTTAAACAAAGGTTTGAAAATAACCGTTGAAAATGTTGAAAAAAATATCATCAAAGTTTTTTGTTTTGAAGGTGGACTAATAGACTATGTCAAAGAACTAAACAAAGGTAAAAAATTAATAGTTCCCGAAGTTATTTATGCAGAGGGAGTTTTTAATGATAAAAACTTTACAAATGGACAAGATGTAATAGTAGAAGTTGCAATGCAATATAATGAAGCCTACACAAATAGTATCGTTTCTTATGCAAACAATATTCAAACAATTGATGGTGGAACACATGAACAAGGTTTCTATGATGCATTAGTAAGAATTTACAATAATTACGCCGAAACAAATAAACTATTTAAAACTAGCTCAGAAAAAATAACAAGAGAAGATGTTAAGGAAGGTTTAGTAGCAATCATTTCTATTAAACACACAGACCCAATTTTTGAAGGTCAAACTAAAGGAAAATTAGAAAATAAAGATGCAAGAATCGCTACAAATAAAATTCTTTCAGACTCACTAGAACGTTATTTGAATGAAAACCCAGAAATTGCAAGAGCAATAATCGAAAAGTGTCTTCTTTCGCAACACACAAGGCTTCTTGAAATAAAGGCTCGCGAAGCTTCTAGAAAAGGTAATGGTTTAGATTTAGGTAATCTTCCAGGAAAATTAGCAGACTGTTCATCGAAAAATGCAGAAATTAGAGAATTATTTATTGTCGAAGGTAATTCGGCCGGAGGTTCTGCTAAAATGGGTAGAGATCGTTCTATTCAAGCTATTTTGCCTCTACGTGGTAAGGTTATAAATGCAGAAAAAAATTCGTTTGCTTCTGTCTTGTCAAATAAAGAAATTGCAACAATGATTCACGCCTTAGGAACAGGAATAAATACAGAATTTGATATTAACAAATTAAAATATCACAAAATTATCATTATGACAGATGCCGACGTTGATGGAGCACACATTACTACTTTATTATTGACATTCTTTTACCGTTATATGAAACCTTTAATTGAATATGGATTTGTTTATTTGGCACAACCTCCACTATATAAAATAACTAGCGGAAAAAATGTTGAATATGCATATAATGATTTACAAAAAGAACAAATCATGGCAAAATTAGAAGATAAAAGAAATGTTGCTATTCAACGTTACAAAGGTCTTGGTGAAATGGACCCAGAACAACTATGAGAAACAACAATGGATCCAGAAACTAGAAAAATGCTCCAAGTTCAAATAGATGATGCAGCAATTTGTGATACAGTATTTGCTACATTAATGGGAGAAGAAATAGAACCTCGTCATGATTTTATTCAAGAAAACGCAAAATACGCAAATAATATAGATATCTAATATGCTAAAGCCTCTAACTAATCAAGGTTTCACAAACAAAGTATTTTACGATGATGAAACTAATAGATTTATAAAAATAAAATCATATGATGGATTTAACCATAAAAGCGATGCATTCTTGCTAAATAATTTAGATTTTTGTCCAAAGATATTTGTTGATAATAAAAAAGAACTTCAAACCGAATGAATTAATGGAATTACATTAAACGAAAGTCTTTTGACAGATGATATTTTAAAAACTATTGGAAAAAATTTAATCACTCTACATAATTCAAAATTGAAATTTTATAAAGAAAATCAAATTGCTAGAAGATTTAATATTTATAGAAAAAAAATTTCTAGTTTAAATAGAGAAATTCCAATCCTAGATAAATACTATAAAAAAATTAATTTGTTTTTAAGAAATATCGACAATTCCGCCCCTGTCCATAATGACCTTTGACTATTCAATATGATAAAAGTAAATAATAAAATTTATTTTACAGATTGAGAATATGCCACAATGGGCGATGTTCATTTTGACCTTGCCTATTTTATTGAATCAAGCAATCTAAATGAAAAACAAGAAAAAGTGTTTTTAGATGCTTATGGTGATGATTTTGAACCCAAATATTTATTTATTCACAAAATTTTAGTAAATGCCTTAATTGTGCTATGAATAAATGCCCATGAAATACCTCCATTTGATGATAGTCTATATCTAAATAGAGTTGAAAAATACATGGAGCAATTAGAAAAAGAAAAAGAGTAATATGTTAAAAAGAGATTGTTGTTGTGGTTGCAAGTCAGCTGAGTTCTGCACATGCAAAAATAAAAAATCATGTATTCCTAATAATTTATATGAAAAAATTAGGAATGTTTTTGGTTATGAAGTTTTTGAAAAACTTAATAATTTAAGACCTTATTTTGATGATTTACATAGTAGTACATATATTGGAAAACTAGATGATGTTTGAGTTCAAATAAGAATTCCTTCTGACAGTAAAATAAATTATGATAACGAAACAAAACTAGTTGAAAAGTTCAAAGATTATTTTTATTACAAAGATGGATATATTATAAAAAAATGATTCCCCGGAGTAGACTTGTTTAAAGTTAAAATTGATAGTGGAATTAAAAAAGCAATATTTAACTGCGTCAAAAATTTTCAAAACCTTAATGTAGATAAGATAGAAAAATTCGATTGATTCAAATATCCTATTCAAGATGCTGAATATAAAGCATTAGTTAAAAAATATTCAAAAGAACCTTTAGTACTAAGCCATAATAACCTAAAACGTCAAAATATTCTAGTTAATAAATACGGCTTCATTAAACTAGTGGATTTCGAATATGTTGCTTTAAACAATAAATATGTAGATCCAGTAAGTCTTTATTTATTTTTAGGTATTCCAAAAGAAGATATAATTGATTTCTTTAAATTAGACCCTTCTATATTTGATGATTTTATTTTTTTAATGAGAGTTTACAATGAGGCAATGTATTTAAATGATTATTCAAAAAATAATCCCCAATCGTTAAACCCTTTCGATTCAAAGAGTTTATATAGCAACAAGGATTTCTTAGAATTAAATAGATTCATTGTTCAAAAAAATCATAATAACTTTGACAATAAATTAAATATTTCTAAAATTGAAAAATTTTATTTTGTTCCACTTTGTGTTTACGAGGATGAAGATAGAACCATATGAAAATGAATCAATTCTAAACAATTAAGTTCATTCAACCACCATCAAATTAAAGTTCTTGCAAAAGCAATGAGAACACTTCATGATTCTGATGTAGAATTTCCTGAATACATTTTATCAAAGAAAATTAATTGATATTTAGATCATATGGAAATAAAAACCTTATTAGAAGATTTAAAAGGCAATAAAAGAATCAATGAAATTATTAAATGAATCAAACAAATTAAACCAGATGCAAATTGTCATAATAATTTAAATTTCAATAACATTTTCTTTAATAGTAGCGACAATTTATATATTATTGATTGATCAGTTGCATATAGAAACAATCGTTATTTAGACATAGCTTTCTTATTTGAAAATACTCAAATGACACCTGAATTAGAATCTTTATTTTGAAAATCTTATGGTATGATATGTCCAAAAGACTTTTATAAATATAGAATTATTGTTCACTTTACAGCATATTTATATAACAAATTATTAAACACTGATTTTAACGCTGCTAAGGTTAACACTAAAAGAATAAATGAAATATTTGAAAAGTTAAACATTAAAGACTAAGGACTAATAAATGAAAAAAGGCAAAGCAAAAGAAACAAAAACTATTGATGTCGACAAGCAAGAAAAAAAATCAATTAGAATTCGTAGAGTAAAAACCGGATTTCTATTTTCAGGAATTTTAATTCCTTTAGTTTGCGTTGTCGCAATACCACTTTCATTAAACAAGAATTATTCATTAAAACGCACCAAATATTTTACTGACGATAGAACTTTATATTCAATTGACAAAAACACTATAAAAGAAAATAATAAAACTTATAAAATACTTACCCTAGCATCTAAAAGTCTTAATGTTTCTGATATTATGAGCGAAAGCGCAAGATACATGAATATTCCACAAAAATCATTTAATAATTCCGTTACATATTCAAATAAAATCAATATTAGAACAAACACCAACTTGTTAAAAAATACAAAAATTAATGAGACAAAGGTTACAAAAATAACTAATAGTAACCAAACTATTTTTAAATTAGATTTAGTTCAACTATGAAATGAAATAAACTCAAAATCTAATAATAAAATATCTGAGTTTGATTTTTTAAGCAATATCGAATCAATAAATAATGCCATGACTTACTCTTATATAAAAAATAACAAACTCTTTTACTTTGACAATAAGCAAGGATATTCTGGATATATTATTCAAAAATTCTTTGAAAATTTTGATGTTGAGATTCGTAAAGAAAATCTTAAAAATAAAAATGTTAATGTTTTAGAAGCTTTTATTTCTAGCACTATCGAATTAAACGAATCATTAACCGAAATTAAAAAAATCACTTTTGATACTAAAATTACATTAAAATATGAAATATAAAAACAGGCAAACGCCTGTTTTTATATTTATATATTCTTTGAATTATTTTATACCGTAGTGAATCTTTGTAACTTTTTCTAAAGTATCTAGAGGTAAGGCACTATTCTTTAATAGAACGTCAAAGAATTCTTTTATGTATTTGTGGTTTTCTTTTTCAACAAAGTCTCTTCTTGATAGTCCTAAGTGTTTACGTACTTGGTCGTATAGAGATAGCATCTTTTCTTTACCAGCATTGTATGAAATTGCTTGTCCAGCTAGATTTAGGTAACGTTTTGATTCAGACATAATGTCACCAACACCTAATGCTGAGTTTGCTGACATAAATCTTCTTACATCATTAATTGATGCATTTGCAGGTAAATCAGATTTTGCTTCAATTCCTTGTCCATGGTATGCAGGGTCAACTGCTCTACGCATATTACGTAATTGTGCTTCGTTTAATGCTCCAAAGTATTGTAACATGTTTGTTAATTTTACTGCTCTTTGTGCATGTTCCTTATCATCTTTTTCATCTTTTTTATCAGTTAATGTTCAATATACTCCACCATGTAATTCTTTGATTTGTTTTATCATGTCTGGAGTAATTTCATCTTTTGTTTTTGCTGTAAAGAAGCTTGTTATTCCTTTTGCATGGCTAAAGTCAGTTGGTTTTGCATAGTAATCGTTATCTTTGTTATCGTAGTCAGGAGTTCCGTAGTATCCTGCTTCAATTCCAAATCATTCCATAAATAATGCTCAACCTTCAACGTATGAAGTAAAGTCAAATGCATTTGCACCTAGATTTTCGCCGTTTTGACTTGCTAAGTGTTGACGTGCGTAGTAAATTTGGTTGTGGTGTCCCATAATTCCTTCGTGGTTAGCGAATGAAGTAACTGATCATTTTGGCAATGAGTAGTATGGGTCACAGTTGAAGTTAAATGCACCTTGAACAGAAGTTACATTTCCGTTTGCATCCAAAATACGTGTATCTGAGTTGTATGCTCCAACACCTTCTTGATCACGTCTTTCATATAGATATGTTACTGCTTTGTAGTCAGGAACAGTTTTACCGAAGAATGATTTTCCATTCTTGATGGTTGACTTCATGAATTGGTCATAAGCCTTAAATGCTTCTAAGGCTCCATAGTAGAATTGTTCGTTTGTGATTGAACCATATGCTTTATCTTTGTTTTCATTTCTAGTTAAGATGTCATATCCATAAGCACTTAAATTGGTCTTGTATTCTTTAAGAGTTGCATCTTCTGATAATGACTTCTTGTAGGTATCTGTATAGAAACTTTTACCTTCACGACCAAAGAAGAATTGTTCTTGGTTTAATCATTTTGCAAAGGCTTGTAAATCGATGTTACCTTCTGCATCACGAATTGTTAATTTAAGATCTTTTACTTCTTCTGGTCCAATACCATCAGCGTCATATTTGATTGTGGTTTCTCATGCTCCATTTTCGCCTGCTATTAATTTAGCAACTTCTGATGCAATAGCAGTCATGTTCTTTGAAACACCTTTTGATGTTTTGTATCCTGATTGATATACATCGTTTGGAGTGTCTTCTGATGTGGTTGCCATTTTTGTGATTTGGTCATAAATGGTCTTTCCACTTTCAGTTTCGTCGCCACCAACTTGTGAGAATCCAACACCCTTAGCTTCTAAGTCAAATTTGGTAAAACCTAAACCATAAACTGATCTTTCAATAAGTTTTCCGTCTTCTTGTTTTGAAACTGATATTGTATTTTCTGTTTCTTTGTAAACATTTTCTTTATACAATTTAAGTTCTTTTAGATTTTGACCTAAACCATGTTTTGAAGCGTTATAGTATTCTGTAGCATAGTATTTTCAGAATTCTCTTAGATCTTTCTTGTCTGTATCTGTGTTTAAATCAATTAAATCTGGAACACTAATTTCTGTTAAAGTGTCGTCGTCTAAGAATCTATTTAATTCATCTGCAAAGAATTCTCTAAGTGCTTCTTTAACATTATTTTTGATAACAACTTTTGATAAAACTATATCTTTTTTAACAGCTTCTTGTAACCTTGCTTCTGCTTTTGCTCAGTCTTCTTGATCTAAAATTGTTCCATAGAATGAGTTTGCAGCATAAACATTTCCTGCTCCTCATCCATATTTAGATAGAACATTACGTCCACCTGCTAGATAGTTCTTTTGAACCATTTGTCAGCTGTAAATTGTTGCATCTTTTCATGCTGTTGTAATAGCATTTAAGTTTTCTTCTTTGATTAATTCTAGCAAGTTAATGTATTTTTCATAGAAGTTAGCAATTGCTTCAACTTGTTCTCTAGTTGGTTTTTCAAAATCAACATCTTTTAATTCTTCAACATCAGCAGGATTGTTTAATGCTGAGTCTAGTTGGTTTCTAACTTCAACGATATAGTCAGCTACAATGTCATATATTTTTTGATCTTTCTTTGCTGATAAGCTTGAAACTACAACCTTAGAAAGTTCAAATTTTTCTGTAACTTTATCGTTTATAAAGGCCTTGGTTTCTAGAATTTTACGTTCTAAAACTTTTATTTCTTTGCTAATAGCTTCCTTTTTATCTTCAGCAGCAGAAGTTAATTCATTTTCCTTAGCTGTTTTCTTAGCATTTAAGTCATTTAAATCTTTTTGATAAGCCTTAATTTGGTTTTCAACTGACTTGATGTTTAAAACACTTCTTCTTACTTCTTTATTGTGGACACCAGTTGAAATTCCTCATCCAATTAAAGGAATTCCGGCAGTTACAACTGCAGCTCCAAGAACAGCAGCGGCCACAAAAATTTTAGATTTTTTTGTCACTTTTTTATTTCCTTTCACTTAATATAAATGTATATACGTATATATTTATATTTGAGTTTTTTTATATACATATATATCTTAAAAAATTGTTGGTTATTTTAAACCTATAGAAAATTATAGAATGTATTAAATCAATGTTTAAATATATAATGTAATAATAAAATTTAGTAAAAAAAAATAACCATAAATACTTTTAAAAATGAAAAATAAAAAACAGAAGATTTTCTGCTTTTTGGTTTACTTGGTATGGCGGATAGTAAGGGATTTGAACCCTTGAATGTTAGAAAACATTATTAGTTTTCGAGACTAACCTCTTCAGCCTCTCGAGCAACTATCCGTTAGTTTAATATTTAGATTTTTATTTTTTTAACTAAATTAATCTAATTATTAAATCAAAACAATTATAATAAAAATGAGTTAATATACACAAAAATAGTAAATGTTTATTTTATTAACTTATCAAGTATATAAAATAAATTCAAATAATATTGTGAAAGTAAAATTTTATATATTTATGGTTTAACTTATTATTTATAAGTTTGGCTTAATCAACTTGCAATATTTATTATTGCAAATAATAATATAAAAGAGGAAAAATGAAAAAAATAATTCTTACAATTATCGATGGACTTGGTCTAAGAAAAGAAAGGCAAGGAAATGCTTATTTGCAAGCAAAACACCCTTGCTTTGACTATCTTTTTTCAATGTGCCCTAATTCTGTTTTACAAGCATCTGGTCAATACGTTGGTTTGCCAGAGGGACAAATTGGTAATAGCGAAGTAGGACATTTAAATATTGGTGCAGGTAGAGTTGTATATACGGGATTATCTTTAATTAATAAGGCAATTGAAAATAACACTTTTAAGGATAATGAAATTTTAAATGATGTCATTGATAAAACTATTAAAAATAATACAACATTGCATGTAATGGGTTTATTAAGCAACGGCGGTGTTCATTCATTAGATTTACATTTGTTTGAAATCCTAAAATTAGCGCATAGCAAAGGATTAAAAAATGTAAGCGTTCATGTGTTTGGTGATGGAAGAGATGTAAAACCACAATCAATAAAAAATTCTCTTGAAACCTTAAAAGATTTATGCCAAAAATTTGGATATAAAATCTCTTCTATATCTGGCAGGTTTTATGCAATGGATAGAGATAGTATATTTTCAAGAAATCAAGAAGCATATGATGCTATTTTAGGGCAATCAAAAAATGTTATTGACAACATTGATGATTACATTGAAAGTCAATATACAAAAGGAATATTTGATGAATTTTTTGAACCTGCCCAATTAAAAGATGGCTCATTTGTTAAAAATGGAGATTCGATTATATTCTTTAACTTTAGACCAGACAGAGCAAGACAGCTAAGCCATATGTTTATAGGTTCAAATTTATACACATACGAACCAAAAAACAAAGTACTAATCGATAATTTTGTTTCATTGATGAAATATGAAGGTATAAATAGTAAGGTCGCATTTAAAGAAATGGAAGTTGTAAATCCACTAGGCAAGGTTTTAGAAAGTAATGACATTAAACAATTAAGACTTGCAGAAACTCAAAAATATGCCCATGTAACATTCTTTTTTGATGGAGGGGTTGACATCGAATATAAAAATGAAAATAGAATTTTAGTTGATAGTCTTAAGGTTGATTCATTTGCTGATTATCCACATATGTCGGCTAAAGAAATTACAGATTCACTATTGAACAACATTGAAAAAAATGATTTTATAATAATGAATTATGCAAATCCTGATATGGTGGGCCATACCGGTAATTTGAACGCAACAATTGAAGCAATTGAATTTTTAGATTCACAATTTCAAAGAATATTAGAATATGTATCATTAAATCATGAAAATGTAACCTGATTTATTACAGCAGATCACGGCAATGCTGAAATTACAGAAGATGAAAATAACAAACCAGCAACAAAACATACAACAAGCCCAGTAATGTTTATTTGTACAGATAAAAATGTTAATTTAGGAAACGGTAGTCTTTGTGATGTAGCTCCTACAATATTAGATTATTTAAAAATCAATAAACCAAAAGAAATGACTGGAAAATCATTACTTAAATAACAAAAAATAGATTCATTATTCATTTGAATCTATTTTTTTGTTATTCATTTTCTTCACTAAAAGATGAATGATATAGCTTTTCATAAAAACTACCTTTGGTCAATAGTTCTTTGTGTGTACCTTGTTCAATAATTTTTCCATCTTTAACAACTAAAATTTGGCTAGCATTTACTATTGTTGAAAGTCTATGAGCAATAACAAATGAAGTTTTTCCTTTCATTAACTCTAACATTGCGTCTTGAATATTCTTTTCTGTTCTTGTATCTACATTAGATGTTGCTTCATCTAATATCAAAATATTTTTATTAGCAATTATTGCTCTTGTGATTGCAAGCATCTGTCTTTCACCTTGTGAAAAATTGCTTCCGCCTTCTTCAACTATTTCTTCGTAACCATGTTCAAGTTGTCTTATAAAATTATCGGCCTTTGTAATTTTGGCAGCATTAATAATTTCTTCATTGCTTGCACTAGGATTAGCATATCTTAGATTTTCTAAAATTGTTGTTTTAAATAGAAAAGTATCTTGCAATACTATTGAAATTTGATTCCTTCAACTAGATTCATTTATTTCGCTTGATTTAAAATCATCAATTTTTATTTCTCCTTCAATAGGCAAATAAAATTTGCTTAGTAAATTTATTATTGTAGTTTTGCCAGCGCCGGTAGGTCCTACAATAGCAAACACTTGACCTTTTTTTGCTTTGAATGAAATATTTTTCAATTGTAAATTGTTTAAAGAATTATCATATGAAAAACTTACATTTTTAAATTCAATATCACCATTCACATTTGATATTGTAGCAAGTTTGCTTTCATCTATAGCCGGATGCATTCTAATTAAATTTAATACTCTTGTTGTTGATATTGTTGCTGTTTGAATCATTGAAATATTTTCAAAATTTTTAATTATTTGACTAGAAGTAATTTTTATATACAAAATAAATGATGTCAATGTACCGACTGTAAAATTACTATTTGAGCCAGTGCCAATTCCAGACAATGCAAATGCTCCGCCAACAAAAATTATTATTAATTGCATTATGAATGTTATTGCATTTCCATATGGATAAACTATTCCAGCATATATATTTGCTTTTAATGAAGGTTTATATAATTTTTCATTATATAAATCAAATTGTTCGTTAATAATTTGTTGACTATTCAATGAATTTATTAATTGGTGTGCTGCCAAATTTTCTTCAACAAAACCATTCAATAAACCTATATTTCTTTGAGTCTTACCAAATAATGGAATTGCTTTTTTAATTAGAACAAAAGCCAATATCAACAAAACAGGAAATAAGGCAATATATATAAGTGTTAAATATGTAGAATAGTAAAACATAAATCCAAGCGATGTTAAAGCACTAAATATCGCTGCTAATAACGAGTTTAAGGTTCCACATATACCATATGAAACATTATCAATATCATTTGACAATATTGACATTAATTCTCCCGAGCTAACAGTATCAAAAAATTGGATTGGCATTTTCATTATTGATTTATAAGCCTTACTCCGCATTATTTCTGAGCCATAAATTCCTGCCTTAACTGCCATTGCTGAACATATAAATGAAAAGACTTTTTCTAAAATATAGCCTAAGATCAATATACCGATTGCAATACCAAATTCAAATAAACCATTAGAATTGCTATTTCAAAACTTAAAGTTTAAATAATGGTCGATAATATAACCCAAACCGTATGTGGCAGCAGTTAAACACAATGAAGAAATTAAAGTAATTATTATTATTAAAAAAACATATCCACGTTTGTAATTTAAATATTTAATTAATTCTTTAAAACTTTCCGATTTTATTGCGTGTTGTTTTTTTCGAAATGCTTTTCTTTCAAATTTAGATACTTTTTTTAAATCAATATCAACTTTTAGTGGATCAAGAATATCTCTATTCATTATTACCCCCTAACTGTGAATATGCAATTTCTTTATATAGACCATCTCTAGAAATTAATTCCTCATGATTGCCACGATCAATAATTTTTCCTTGGTTTAATACTAAAATTTCATCTGCATCAATAATTGCTGAAATTTTTTGAGCAATAATAACTAATGATATTTTTTCATATTTAAATTCTTCTTTAATATTGTTTCTTACCATAGCTTCTGTTCTAGCATCCAATGCTGAAGTAGAATCATCTAAAATCAATATTTTAGGTCGTTTAATTAAACCTTGGGCAATAGATAATCTTTGTTTTTGACCGCCTGATAAATTTGCACCCTTTTGTTCAACAACATGTTCAAATTTTTCTTTAAACTTATTGATATATTCAAAAGCGCAACCATTTTTTGCCGCTAATTCAATCTCTTCATCAGTGGCATTAGGTTTAGCAAGCAATAAATTGCTTTTTATTGTGCCACTTAATAAGCAAGGGTTTTGATAAACGTGAGAGATATTCTTTCTCAATGAGTCTGTATCAATTTCTTGAATATTATGCCCATCAATTGTTACTAGCCCGTCTTGAGTTTTAAAATCTAAATTTAACAATTTTGCAATAGTTGATTTTCCAGAACCAGTAGGGCCAATTATGCCTAAAACCTTACCAGGCTTTAAACTAAATGAAATATCTTCTAAAACATTAGATTTGGCAGATTCATAATATTTAAAAGATACATGGCTAAATTCAATTTCTCCGTTAACAATTAGCTTATCAGATTTGATTTTTGCAATATCTGGCTTTTTGTTCATAATTTCAAAAATACGTTTTGAGGATACATTTGCCTTGAAAATATTAAATGTAGTAAATGATGAAGCAACAACTCCAAATGTAACAGTCATAACATAATTCATAAATGTTACCCCATTACCTATTGATTTTGAGAAATCAGCAATATTTTCCTTGCTAGTTTGCTTCCCTACAAAAAATACAATGAAGGCAATTGCTATATTTGAAATTATTTCAATAAAATTAAATGTTATAGTGAATATTATTCATGATTTGCTTGTTATGGTTCCTCAATTTTTATTAACATTTTGAAATTTATTTCATTGAATTTGTTCCATGTTATATGATTTAATAACTTTGGCGCCTAAAATATTTTCTCTACTTTCTATGTTAATTTTGTCATACATTTCTTGTTCTTTTTTTTGAATTGGATTTCAAATGACACCCGAAATTACCATAGTTAAAGTTAATAGTGGAATAACTACTAAAAATATCATTGACAATTGAAGATTTGTTAATAAGGCAAATACTAAACCTAAGACTAGATTAAACGGCCCTATAATTAGCATCCTCAAAAAAGACATTAGTCCGGTTTGAATTCTACTAATGTCTATTGTAAATCTCGTTAGGATCTTGGCATTTGAGAAAGCTTCAATATCTTTTTGAGAAAGTTCGCCAAGATGCTTTCATAAACAACTTCTTATTTGTTTTGATCCTGCTTGAGACACATATGATGCAAGCAATGAAGCACACATTGCTGAAATAGTTCCTATTATTAAAAACAATGCAAAATATCCTCCCAAATGCATTAACGCACTCTGATGGCTTTGACATAAAAAATTAATTCTTAGAATTTCAATTGTTATTGGTCTGCTTGTTGACAAATTATTTTGAATTAAATATGCATTTTCACCAATTAACCTTGTCAATTGTCCCAATAATATTGGCAATACTAAAAATGAAACTACTTGTAGCAATGTTAAAGATATAGTTAAAAATGACAAACATTTGAATTTGTTGGCCATTAATTTATAAAGTTTAAACATTGTTTCCTCTTTCTTTTTAAATTTGTTTTAATTATTTAATTTAAAAAGTTACCTTATATTATCATCAAAAAATTGTTTAACTTCTTTTGCAATATAAAGTTCTTCATCTGCCCTTATTATATAAATAGGAATTGAAGAATTTTTAGTTGAAATTAATTTGTATGAATCAAAATCTCTTCCTTTATTTAGTTCACTATCAATTTCTAATTTAACTAAATGAATTTTATCAATAACTGCTTGGCGAATATATTCATCATTTTCACCCACACCAGCCGTAAAAACAATAGCATCGATTTTTCCTTCAATCTTATTCATGTAATTAATTAAATAATCTGCTATTTTTCTTACATATAAATCTATTGCAAATTTTGCTCTTTCATTTGTTTTTTTAGCTTCTTCAACGTCACGAATATCAGAAGAAATTTTTGAAACTCCCAATAATCCTGATTCTTCATTTAACATTTTTATTGTTCTTTCAATATCTCAATTTTTATGTTTCATAATATATGGAATAATTGAACAATCAACATCACCAGAACGAGTGCCCATCATAACTCCCGCTAGTGGTGTAAATCCCATTGAGGTATCAATTGATTTACTATCTTGAATGGCTGCTAAACTTGCACCATTACCGATATGTAAATTAACAAAATTAACTTTGTCTTTATTTAAAATTTCTTGCAATTTTAATGTAATAAAGTGGTGATTCAAGCCGTGGAAACCATATTTTCTAACATTTAATTCATCGGTTATATTTGCATCAATTGCATAAGTATATGCTTCTTTTGGAAGGGTTTTATGGAATGAAGTATCAAAGTGGAATGTCATTTTAGCAAATGGTAAAATTTCTTTAAAGGCTTTTACAGCTTCAAGAGCACCAGGATTATGAACTGGTGCTAATTCAATAGCATCTTCTAAATATGCAATAGTTTGAGGGGTAACTTCTGAAGTGTCTTGTAGATGTATTCCACCATTAACAATTCTAAAAGCAACATATTTAATTTCATTAAAATCTGAAATAATATTCTTATTAATTCATAAATTAACCAATTGTTTAACTGTTTCTAAAAAACTAGGTAATTCAATACTTACTTCTTCTTTTTTGTTATTGTAATTTAAAATAATAGAACTATTTTTAATTTTAATTCTTTGTGCAATTCCTTTGGCAATAATATTTAGATCTTTTGAATATAAGGTTCATTTAATTGAACTTGAACCTGCATTAATAATTAAAATTTTTGACATAATAGCTCCTTTATAATTTATAAAATTATATTAAAAAAAATATCTTTTAAACTTAATATGATTAAAAGATATTAAAAAAGATATAAAAAAAAGCAACGTCCTATTTTCCCATTGAGGTATCGTCGGCACTGAAGGGCTTAACTGCTGAGTTCGGAATGGTTTCAGGTGGACCCCTTCGTTATGGTTGCTACGTGTATTATTATATACTATTTTTAAATATGTAATAAAAAATTTTTATTTTTTTTATTACTTTTTAAACACTCTTATTTTTGCAAGATTATTACCAATATTTTTTCAATAATATCCTTCTTTGTCTAATAAATCTTCCGTTATTTGACGAATAATCCCACTACCAATGCCTACAATAATATCGAAGTAATTATTATATTCATCTTGTTTAAATTCATACAAAGCGTTTAAAATTGCTGCTGTTGCAGAGTTTACATCATAACCATGAAGATCTAACACCATTGGAAAAAAATTGAACTTCATTTAATATACCTTCTGACAATTAGGGCAAACATATGTACCACGTTGGTTAACTTTTAATTTAAGTGTTTTTTGGCCACAAACCTTACATTTTTTTATAGAATCACTATGAATATTTAAAAAGTCTTGATATTTTCCTTCTTGTTTATTTAATGATTCATAAGAAAATAACGTTGTTCCACCTAATTCATAACTTTTTTCCATTATTTCATTTGCATTTTTTATTATGTCTTTAAGTTGCTCTAGTGATAAATCAGAAGCTTTTGAAAGAGGATGAATTTTGGAAGCAAACAATGCTTCATCAGCGTAAATATTTCCTATACCAGCAACAAAACTCTGATCAAGTAATTTTGTTTTAATGGGCATTTTTGAATTTTTTATTTTTTTAAATAATGCTTCAATGTCAATATCTTTTGGTTCAAGTCCAAGCAAAGAAATTGGAGGAATTTTGTTATAATTTTCTAAGTTTCTTAGCATATATGTGCCAAATAAACGTTTATCTAAAAAGTGCAATTCGCTATTGTCGCTAAAAACAAATCTAGCAACTAAGTGCTTATCAATTTCTTTTGGAGGGTTTAAATAATAACGATATTTCCCTTCCATTCTTAAATGAGAAAGTAAAACTAATTTTTCTGATAAAAAGAAAATTATATGTTTGCCAATGTTATCTATTTTTTCAATTTTTTTATCTCTTAAAATTGAAATGAAATATTGAGGGCTAAACTCTTTAAAAATTTTGGGTTTGTATATAATCAAATTTGTTATTTTTTTATTTAATATAGTATTATTAAGGGCTTTAACAACAACCCTAACTTCAGGTAATTCAGGCATAGTAAAATTATAATACTTTCGAATGACATTACTCATATGATTAACTGCAAAAATAAAAGTGTTATATAATTTAAATTATGATAAATAAATATCAAAAATTAATTATTTCAACTACAGACACAGTTTTAGGAATTGGCGGCCCTGTGTCAAAAGAAGTTGAAGACCTTATTTATGAAATAAAAGGTAGAGACCGCAATAAAAAATTAATAATATTAGTAAGTTCAATAAAACAAGCAAGGCAATTTAAGGAATGAAATTCGCAAGCAGATGAATTTGCTAAAAAATATTGGCCTGGTGCAACTACCATTGTCGTAAATAATCAAGGTTTTAGAATGCCAAACCAAAAGAAATTGCTTGAATATATAGAAGTAAATGGTCCAATTTATATGACAAGTTGTAACTTGTCAAATGCACCTGTATGTAAAACTATTGAAAATGCAAAGCAATTATTCCCCGAAGTTACTAATGTATATAATTTTGGTCCTATGACTCAAAAACCTAGTCAAATAATTAGAGTAGAAGATGGAGAAATTTTAAGAAAATAATATGAAAACGACAGTTAAAATAACAAGCGATCACGCAGGATATGACGCAAAAATAAACCTTGCAAAAAGATTGGAAAATGAAGGCTACAATGTAGAATTATTTGGCTCAAAAACAAATAACAATAGTGATTCTTATGCAGATGCTGCTATTGAATTAGTTAATGAAATTCACAAGGATGGCAATCCTGATGAAAATAAAAATGTAAAATATGTTGCATTTTGTGGATCAGGTATTGGTATTGAAATGACTTTAAACAAATTTAAAGGCATAAGAGCAGCAAGAGTTACAAATGAAAACGAATCTCACCTAGCAAAATTGCATAATAATGCTAACGTACTATGTATGGGTGGAAGATTGTTAGAAGGTAATGAAGTTGAAAAAATTTTTCATAGCTGAGAAAACACCGAATTTGAAGGTGGCAGACATCTTGAAAGAATTAAAAAACTAAGCTCAATTGGTGAAAACTAATAAATTTATAACAAAAAATAGGCTCATTTTATTGAGTCTATTTTTGTTTTTATATATATTTTATTTTTCAATCTTAGAAGTATTAAATCTTCCAGATACGTTTTCTACTTTTGATGAAGTTATTCATGCATTACTATCGGCAGCCAAAATTTCTTTCTTAATCATATTTTGTTCCAAGAACAAACAATATGTTTCAATTTTAACCGTTTCTGTATTGGTATAACCTCCCAACATACGATCAATAGTGTAAGCGTGTCAATAGTTAATTTCTTTGAATTTGTTTAAAATTAAATCAGGATTCTTGCTGTAAATTTCTAGTTTTACTTTTCTGTATTTTGGATAAATTGATGCCATAATAAATGTATATAAGAATATATATACAAATGTACAAATAATTCTTAATAATAATGCTGCATGGTTTATTGGATTTCCATTTTGAGCATGTGATATTACCCCAGTAAGTTCGCAAATTGAAATAACAACTATTGAGAATACTGCAAAGCAAATTGCGACTATTAAAGAAATTAAGCCTGGTTCTTTTTGTTTAGTTCTTGAAATGTAGTAGACAACAAAGTCTCCCCCTGCTGTTGAAGCAGAATTTTTTCAAGCAATTCCCCCTGCACATCCTCCACATAATGCTCCAAGCACTGCAAAAATGATAATTGGTCAAGTAGGATTGTTATAACCATGTATTAAATGTTGATAGAAATATCAATAAGACATTGTTTCTTGGTAGCTTAGTTGCACGCCGTTTAGAAGTATTCCATTATTTCAAGAAGGATTAAATCCAGTACTTTTTATAGTATTTCAAACGTTTGAATAATGTGTTAGATAGTTTCCATAGACATCCCATGGTATTAATACATTTAGAGAGTTAGCAACTTCGCCATGTGCTGGAGCTAATGTTGCAGAATGAGGAGCATACCAGTTTACATAATAAAACGTTATTTGACTAAAGAAGTGGCCAACTTGTGGAATTAACAATATTGATTGAAAGATAACTTGTCAAAGTAACCAATAAGTTGTTAATATCATAAATAGTCTAGAATTTTTCTTTCAAAATGCAATTACAAAAGGTAAATTCATTGCTATATAAATATAAGCAAAATATGGTGCAGTTTGAGTTAATGTTAGAGTAATGGCTTGAACCAATGCAGATAACCCAGTTGCAACAGTGGCAGCCTTAGCCAAAAATGTTGCAACACCAATATTGTATAAAACGGCTGAAATAATAATCATGACCATTTTTTTAGGAAATTTCTTTCAAATATTAAAGAAATTTACCCCATAAGGATTTATGTCAATTTTGCCGACTGATCCCTTTTCTTTTTTGGTTTCTTTCTTTAAACGTTTACGTTCTTTAATCTCAGCAAGTTCCTCAGCTGTATGTTTCATTTTCACCTCCTTTTTATTATCGTGTATATTCTAACACTTTTTAAGTGTTTATATTTTTTATTAATTATTTTTTTTAATAAAAAATACTTTTATATGACTTTAATAATTAATCTACGTATGAGGTATCAAATGAACCAATTAATCCATGAATATTTGAGATACTTATTCAGGCTTTTTTATCAACGCTTCTAACTTCTTCTAAAATTGATTCTTTTTCTAAATATAGGGCAATTGTTTCAATTTTTCCAAAATCTTGATGGTTATATCCACCAAATCCGTTGAATACATTAAATGAGTGAACATATCCAATTTGTTTGAATCTTTCAGCAACTAGTTCTGGTTTTGTGGTGTAAATATCAATTCTTACTTTCTTGTATTTTGGATAGAACTTATTTACTAACATTGAAAATACTAATAAGTAAATTGCAGTGCCCATAATTCTTACTAAAAATTCTGAAAAATCTCATGTATGCCCAATAATGTTTCTTGTATATTCTAATGAACCTATTACAATTGTTGAACATGATGAGAAGCAAATTGCAACCATTAAAAATACTGTTCCTACTGGTTTTTTATTTTTATATGCAATGTAATTAGAAATAACAGATGTACCGCCAATTGAACCACGTTGTTTTCAGGCAACTATTGATGCAAGGCCTTCCATTATTCCCCCAAGAATTGCATATATCAAAATAGGTCAATTTTGGCCATTATATATTCCATAAATTTTAGTATTTTGACCGCCTTTTAAATCAATACCTGTTAATAGACTAGGAATATTAGCAAGTGATAAAGAATTAGATTTTTCAAAAATTAATTTTGCATTATCAAATACCATTTTTAAATCATTAGTTTTAAATAAGTTGGCTAGATACTTAGCAATAGCTAAATCATCTCTTGACAATGAACTGCCAGTTCAAGCTGTTTTAGTTGATATGCTTAAAGCGTATTCAAAAATATTTTTATAATTTCCTTCATTAATATAATTTAATACTCTTGTATCAAAACCATATGGATCTCAATAAAATTTGTATGTTAATCCGTTATTTGTAAAAGTTTGTCCTATTGGATCATAGAGATTAATTCTTTGATTAATGAAGTTAACAATAGGATTTGATGCTCTTCCTTGGTATTCAATAAATATACATTGTACAATTAATTGGAAAAACATTCAATAATAAGTTATTACCATAAACGTTCTAGGATTTAACCGTCAATAAATAAACATTAAAGGTAGGTTAACTAATACATAAAATAAACCATAAAATTGTGCAGTTGCAGGCGCTGTAAAAGTAATTATTTGAGATAAAGATGAAGTTCCTGAAGCTATTGTTGCTGCTTTTTTTAAAAATACTGTAATTGCAATATTATATAAAAATGCACTTACAAGCATTCAAAACAACTTTTTAGGATATTTTTTTCAGACATTTATTAAATTTATTTTTTGTGGATTTAGAATTTTCATTTTTTCTAAATACCGTTGGTGTTTTTGCTCTTTGGTAAGAATGATTTTATTGATTTTAGTTATTTTTTTATCTTCTAAACTCATATTACCCCTTTCTTTAGATTCATTTAAACTATTAAATTATTAATTTCTCTTTTTTTCATAAATAAACCTGTTGCATTCTGGTTGGAATAATAGTTGTACAATTCCAATAGCCCCATGTCTATTTTTTGCTACAATGACATTGGTTAAAGAAGAACTGCCATTGACAATTTCACTTTTGTCAACAGCTTCTGCATTATCATTGTCATCATTAATGTTGTGCTTATTATAATAATCTTTTCTATGTAAAAAAATAACCATATCGGCATCTTGTTCAATTGTTCCTGATTCTCTTAAATCTGACAAAATAGGAGTTTTATCTTCCCGTTTTTCAACATTTCTACTTAACTGGCTTAATGCTAAAACAGGACATTCTAATTCTCTAGCAAGTTGCTTTAAGGATCTCGAAATCTTGCTTACTTCCAATTGACGATTTTCCATACTTGCTTTTGAAGAATCTGACATTAGTTGTAGATAATCAACTACAACTAGATCAACTTTATTGTTTCTAGCAAATCGTTTTGCAATGGTTGTTATATCCGAAATTGTTGATGTAGGTTTATCATTTAAATACATTTTTCATTGTTTCATTTCTGAAACAGTTCAGGCTAGATTCTTAGCATTTTCTGTTGAGATTTCATTTTTTCTCATTAAACTAATCGGAACCAAGGAATCAATTGACATCATTCTTTGAACAATTTGTGAATTTGACATTTCAAGAGAAAAAAATAGCACCGTTTTATTGTTTTTGGCAACATTATTTGCTATATTTAAAGCAAAAGCAGTTTTTCCCATAGCTGGACGAGCCGCTAAAATTATCAAATCTCCGGGATTGGCGCCTAAAATTAATTCATCCAATGGTGGAAATCCAAAAGAAAGGCCAACGCCAATATCTGATTTTTCTTTGCTCATTATTCTATTAAATAGTTCATCTGCTTTATTTCCTACTTGTTCATAAGCAGTATTTATTTCTGAAATATCTAGGTTGATTAAATTCAATTGTAAATTGGATAATAAATCTGAAATTTCATAAGGCTTGTTTTCCATAATATTTATTGAACTATTTAATAAATCTCGTAAGCGATCCTTCTTGTATTGATCAATTATTTTCTTTGCATATGACTCTAATTCGGCGCTAAAGCCCGCATTTTTAGTAAGTCAGCTAATATATTCACTACCATTATAGCCAAACGAACCGATGTGATCAATTAATTTTTGGTCATTTAAATAACTCAATAAGTTGGCTATATCGAATTGTTTAGAGGCAATATGAACTTCTGAAATAGATTTAAAAAGAATTTGATTATTCTTAAAATGAAACATTTCAGGTTTTAAAATTTCAGCAATTGTTAAATAACAATTTGGTTCCCTAATTAATAAACCTAATAACGATGTTTCATTATTAATAATTGCTAATTCTCTTTCCTCTTGAATGTTCATTTTTGGCCACCTTATATTTTATTTTGATTATTTCTCTTCTATGATATTTATTTTTAGTATTGCAGTTACTTGGTCATGTAATTTAATTTTTACATCATGCAACCCAAAAGTATTATATGTTACCCCTGATTCAAATGCATGGCTTGGGATTTTAATGTTGTTTTTTATTAATTCTTTTTCAATTGCTTTTGTTGTAACGTTTCCGTGAACTATATTTCCATTAGATTTTAAATAGAATTTTAAAACTAATGATTCAATTAATTGTTTGATTTCATTAGCTTTAGCTATTTGATTTGCTAAGTCTTCTGCAATATTTGATTTAACTCTATCTAAATTTGCCAATGTTTGTTTGTTAATTGGTTGAGCATAACCATTTTTAATTAAAAAGTTTTTTGCAAAACCATCTGTAACTTCAATGATTTCATTTTTTTTATGTTTTTGGTATTCTTTAATTAATATTACTTTCATTATCTTTCACACTCACTATTGCTTGTATTATATTATCAACAAATAAATTCAAATTTTCAGTTGTTTCAGCAGCGGCTGCCCCAAAATGGCCACCGCCGTTAACTGCTTCAGCTATGATTTGAACATTTGTATTAATTCCTCTTGCTGACATTTTGTATGTTTTTGTTCCTGGTAATTTAGCAACAACAAATGCAGCTTTCCTACCATTAACTCTTAATATTTCATCTGACGCTATTGAAATAATATCTGTTGATGCTTCAATATCTTTGTATGCCAAGAAATATCCTTTTTTAATTTCTTGCAAATTGGCTAATAATTCTTGAACAATCTTAAATACTTTTTCATCGAATTTTAATGTATCAACAGATTTATCATTTTGTGCTCCTCATTTTTGCAATATTGATGCTGCTGCAAAAGTTTTAGCACTTGTTTGTTTTTTAAATGTATTAGTATCTAAATAAATTCCATCTAATAAATATTGCGCCGTATCATTATTAATTTTATCTTCTTCATGTAACAATGTGATGATTTCTGTTAATATTTCAGAAGCAGATGATGCCGAGGAATCAATATATACATTTTCTCTATATGCTATTTCATCAGTATTTTTATTTATTCTATGATGATCAATTATCATGATATTTTCTTTTTTTATGTTTTTAAAGACATTAGCATTTTCAATTCTAGTATCATCTGAAGTATCGCAAATTACAACCAATGTTTTTTCGTTGTTGATGCTTTCTGCTTCTTTAGGACTAATAAAATATTCTTGAGTTTCCTTGGACATTTTATCAATAACTCTTTGAGTAGTACTATCAAATGTCTTGTTTTGAATATAAGCATTCTTTTTATAGGCTAATGCTAATTGCAAAATTGCATATGTTGAACCCAAAGCATCAAGGTCTGCATTCTTATGGCCATAGGCTATAACATTTTCAATATCTTTTGATTTTATCTTTTCTAATAAGTTTTGTGCTATATAGTTTACATTTGTCCTTGATAGATTTACATCAATTTCCGAGCTTGAGCCATAGTGTCTAGCTTTTTCATTTTTTGTTAAAACTGTAATTTGATCGCCACCCCGCATTTGAGATTGCATTAGAGCATCTTTTGCTAATTTGACTAACGTATCGTATTTAAAAATACCATAACCAAATCCACCAGAAATTGATATTAAAACATCTTTAACAATTATTTTGTTTTTTACTGAAGTTATAACATTAAAACTATCTCGTTCTAGTTCTTCTAATGTTTCTTTATTTGTTATTAAAAAGAATCTTCCATTTTCGTATTGTCTATAAATAACATTGTATTTTTTAGATATTTCATCTAATAAATTTACTACAGAAGCATAAATTTTAAAAATTTCTTCTTGGGATAAAAATGTTTGATATAAATTAATATTGTCAATGTCCAATTCCCCAAACACAATTCTTTGTTTTTTATAGTCATCTAATAAATTTTGTTGAGCAGTAACATCTCTTAAAATAACTAGATTTTTTTCAAACGATATATGAACTTCGTAATTGCATCCGTCTATTTCATACATGAATTCTTGATTATCTGAAGTTCATTGTTTTATATTAAAAATATCTTTAATGTTTTTGTTGATTAAATTGTTATGAAATTTTATTTTTAAAGATTCTGATGCTCATATAATTTTTCCACTATTTAAAAATATCAAAATTCCCGTACCAAATTGATTAAATTCGCTTTGTAAATAAAATTCCAATGATTTTTTAGCTAATATTGAGTTTTTAAAATAACGTTGCAAAATAATAGTTAAATAAATTGCAAGGCCGATTAAAAATAAAGTGTAAATTGCCATTAACGCTATTTTTGACTGTTGAGTCTTTAAACAAAAAATGCCAATAAAGAATAATATTGTTGGCAAAATAAATAATAGGATAACTTGTAAAGCTATATAAATTTTTTGAATATCTTTGTTTTTCATTTTATATATATAAAATTATAATAGAAAGTTAACAATAGATTCAATTAATGATACTACAAGTCATAAAATTGCATTATTTCTTCAATACCTTGTTTTTTTTCAGATGATACCAAAAAAATATTTTCAACTGTTTTTAAAGACAAGGCTTGATTTTTAACGCAATAACGTTGAGATTGATTTGCCTTGTCGATTTTTGTAACAACTATATCAAATTTTATTTTAAGATCGTTAAGATATTCTATTATTTCTAAATCACTAGCGCTAAAACCAATTTTAGCATCAATTAATAGCAACACTTTTTTATTGTTTTTATCATTTTTAAAATAAAAGTCAATCATAAAAGAAATTTTATCTTGCAATTTCTTTGAGATTTTTGCATAACCATAGCCCGGTAAATCAATAATTATTTTATTTTTGTTACTTTCAAAGTAATTAATTAATTGTGTTCTTCCTGGATAAGATGATGTTTTTGCAATATTGCAAGAAGCCAGAGAATTAATTAATGATGATTTTCCAACATTGGATCTTCCTCAAAATACATATTGTATATTGTCAGAATTAATAAAATCATCGTGGCTCTTACAACTTTTTAAAAATTTTCACATTATCTATCTCTCCTTAATAGTTCATATGCTAACCTAGCAATCATCATTGCATTATCAGTTGTATATTTCATTTCCGGAATTATGGCATTTGAATGGACATTTAAAAACATTTCTCTAATTGCTTTATTAGCACTTACTCCGCCTGCCAAAACAATTACTCTTGGATGATATTTTTTAATTGCAATTTCTAAATGATTTTTTAAATATAAAACTACTGTATTTTCAAACATTGTTGCATATTGATCAACATTGATCTCTTGTTTTCTATTGATTTGGTTATTTATAAGATTTATAACTTGTGTTTTTAATCCACTAAATGAAAAGTTCAATTGAGCTTCAGTATGTGGAATAGTAAAATGTTCTGTGTATTTATCTCTAAATTCCATTCATTTTTTATCAATAATAGGTCCTCCAGGAAAACCCAAATTTAATTTTCTTGCTATTTTATCAAATACTTCTCCGACGGCATCATCTTGAGTTTGACCTATAACTTCGTATTCATTTTTTGATTTATATAACATTAATTGTGAATGCCCACCTGAAACAATTAAACCTAAAGCAGGATATTCAACTTCTTTGCCTATAAAAGCAGAGAAAAAATGCCCTTCTAAATGATTTAAAGGCAATATAGGTTTTTCAAGTAACATTGCTATTGCATTGGCAACAACATATCCAACATGTAAAGAACCAACTAATCCTGGTTCTTTTGTATATGCTACATAGTCAATCTTTTTAATATCAATTATTTTTTTTAATTTATTAATTAAAATACCTATATTTTTTACATGCAATCTTGATGCTAATTCCGGAACTGTGCCTCCATATTTTCTATGAATGTCTATTTGGCTAATTGTTTCCATTCAAATTGGTTTGTTATCTTCTAATAAAGCAAATGATGTATCATCATGAGAACTTTCTATGGCAAAAATTAACATTTTATCTCTCCTATTTGTGGATCATGCAAATAAATTAATTCGCAATTTTCAATATCATTTACTTGTTTAAATTCTTTTAAATATTGCTCTATATTTTTTGAAAAAAGCTCTTTATTGAATAAATCAAAATTGCCTAAGTTTTCTTGCATATGACATTCAAATGGATCAATAATTATTGAATAAACATTGTATTTATTGGCTTTTATATTTATTAAATTGCAATCTTCATATTGCAATTTTTTTTGTGTATAAAATAAAGAATATGTTGGGGCTACAAATAATTTGATATTTGATAATTGTGCAACTGTTTTGGCAAAAACTAAGCCAATTCTTGCACCTGAAAAACTCCCAGGTCCTAACGTTGTATAAATTTCTGAAATTGAACTTAATTTAAAATTATTTTTATTTAATAAATAATTTAAATTTTCAAAAAATGCATCGGTTTTTTTAACTAAGTTAGCAATAAAAATACAATCAATCTCGCAATAATTTTCATCAATTAAGCTTATATAAAAATCGCTTAATGAAGTTTCAATGAATAATTTCACTATTGTTCCTCCATAACTTCAAATACATGGTTTTGTTCATTGTCAAAATAAACTTTTATTTTTATATAATGTTTAAATTTGGGCAATGTCACATTTTCTGCTCATTCAATAATTGCTAATTTATCTTCAAAATAATCTTCATAAGCAAATAAATTTGTAGTTAATTTATAAGCATCAATATGTACAAATTTATCATACACTAAAATCGTATTGAATGTGGGTGATATTACAGTCTTTGGTTCGTTTAATTTTTTTGCTATTTGACTTGTTAAAGTTGTTTTGCCTGCTCCGAGTTCGCCTATTAAAAGCAAGGCTTCTAAATTTTTAAATGACAAAATATAGTCAACTAGTTTATCTAATGGTTCGTTATTTTTATAAATAAATTTTAGATTTGACATGATTTCTAACCTTGATAATAAAATACATTATTATTTTACCATTTATGCACCTTTTTAAGGCTTGTTACAAATAAAAAAATGTATTTAAAATTTAAAAAATAAACATTAAGTATTTAAAACACAAACTTAATGTTTATTTATTTTTTTAAGCAAGATGTTATTTTATTTATATTGAATATTTTCTCAATCAGATTGTTTAAAATCAGTTAAAAATTTTGTTAACAAATCAACGGCTGCCAATAAATCACTTATGCAACATACTCCGATTGGTGAATGTAAATATCTTTGAGGCAATGAAATTGTTAATGTGGCTGCTCCGCCTTTTGAAAATTGCAATTCAAAGGCATCAGTACCCCCACCCATGGCTACATATTTAAATGTTGGAATATTATATTTCTTAGATAAATCAACAAAAAATTGTACCAATTTTGGATCCATCATTGTTCCTCTATCTTTTAATCTTAAAGCAGCTCCCTTATATAAAGAAACATTTCCAGGAATTGTATTAGGTGTATCGTGAGATGCAGAAGTATCAACTGCTATTGCAATGTCTGGATCAATCATAGACGCAACTGTTTTAGCTCCTCTTGTTCCAACTTCTTCTTGAACTGTACCTACTAAATATAGATCAATGTCAAGATCTAATTTTGCAACAGCTTTAGCAACAAATTCTAACACGGTTACACCAGCACGGTTGTCCATTGCTTTTCCACCAATTAAATTTTGATCATTAAATAGTATTGTTTCTCCACTCATGTAAACTCTATCGCCAACATTAACACCATGAGCAATAGCATCTTCATCGCTTTTGAAACCAAAATCAGCAAATAATTCATTTTCTGTAATTGCTTTCTTTCTTTCTTCTTCTTGCATTATATGAATACTTGTATGTCCAAATAATCCAATAAATGTTTCGTTATTTGAAGTAATTAATTTTGCTTTAGTTCCAATAACTGCACATGGTCATACACCACCAACTGTTGATAAATATAATTGTCCTTTTTTATCAAAATTTCTTACTACATATCCAACTTCATCCATGTGTGCTGCTAATAAGACTTTAGGAGCATTTGGATTTTTAGATGGCTTGTGCATAATTAAACAGCCAAAATTATCTCTGCTAAATTCAAATCCTTCATTAGCTAATTCTTTTTTTAATTCATTAACAACTGGTTCTTCATAACGAGACATTGCTTCAATTTCCATATATTTTATTAATCTTTCTTTGAATTGATTTTCTCTTGTCATATTAATTCCTCCTTTATTTAATGTATAAGATATATTTTATAACAAATTGTATAAAAAAATAGCCATTAGGCTATTTGTTGTTCTTTGAAAACTGAATACGACTTAATTTAGATAACTATATAGAATATTACTATTAAACCAATAAATTTATTAGTACTGGTCAGCTGAATGCATTGCTGCACTTACACCTCCAGCCTATCAACCTCATAGTCTATAAGGAATTTTAAAGGGAATACTAATCTTTGAGGGGGCTTCCCGCTTAGATGCTTTCAGCGGTTATCCCTGCCGCACTTGGCTACCCAGCTATGCTTCTGGCGAAACAACTGGCACACCATCGGTGCGTCCACTCCGGTCCTCTCGTACTAAGAGTAGCTCTCATCAATATTCCAACGCCCACATCAGATAGGAACCAAACTGTCTCACGACGTTTTGAACCCAGCTCGCGTACCGCTTTAATGGGCGAACAGCCCAACCCTTGGAACCGACTCCAGCTCCAGGATGCGATGAGCCGACATCGAGGTGCCAAACCTTCCCGTCGATGTGATCTCTTGGGAAAGATAAGCCTGTTATCCCCGGGGTAGCTTTTATCCGTTGAGCGACGGCCTTTCCACGAAGAACCGCCGGATCACTAAGTCCTGCTTTCGCACCTGCTCGACTTGTAGGTCTCACAGTCAATCACACTTCTACCTTTATGCTCTTAGATACGGTTTCTGACCGTATTGAGTGTAACTTTGAACGCCTCCGTTACCCTTTAGGAGGCGACCGCCCCAGTCAAACTACCCACCACACACTGTCCTCTTCCCGGATAACGGGAACAAGTTAGAAGTTCAATGTAACAAGGGTGGTATTTCAACGGCGACTACTCTTAAACTAGCGTTCAAGCATCAACGTCTCCCACCTATCCTACACATGTTAAATCAAACTCCAATATGAAGTTATAGTAAAGCTCCACGGGGTCTTTTCGTCTAGATGCGGGTCTCCGGCGTCTTCGCCGGAACCATAATTTCACCGAGTCTATTGTCGAGACAGTTAAGAGATAATTACTCCTTTCGTGCAGGTCAGTATTTAGCCGACAAGGAATTTCGCTACCTTAGGACCGTTATAGTTACGGCCGCCGTTCACCCGGGCTTCACATTAATGCTTCGCTAATGCTAACACCTCTGCTTAACCTTCGGGCACTGGGCAGGAGTCACCCCATATACATCATCTTACGACTTAGCATAGAGCTGTGTTTTTGATAAACAGTTCCCCCTTACTATTCACTGCGGCCCACATTTTATTGTGGGCATCCCTTCTTGCGAACTTACGGGATGAATTTGCAGAGTTCCTTGACAATAGTTTTCTCGCTCGCCTTAGAATACTCATCTTGGGGACGTGTGTCCGTTCTCGGTACGGGTTTCCTAACACTTAATGTTAGAAGCTTTTTTAAGAGGCATGAAATCATCTAATTCGCTACTCAGTTGCCCTTTTGCTATGCGTCGTAACTCCCAGTTAAGTTATGCGGATTTGCCTACATAACCCAGTTGTTACTTACCCCACAATCCAATTAGTGGTAAAATTATCCTTCCCCGTCACTCCATCACATGTTAAGAAAGTACAGGAATATTAACCTGTTGTCCATCGGCTACGCCTTTCGGCCTCGTCTTAGGACCCGACTAACCCTGGGTGGACGAACCTTGCCCAGGAAACCTTCCCCAATAGGCGTCAGAGATTCTCACTCTGAATCGTTACTCATACCGGCATTCTCACTTGTAAGCGCTCCACCAGTCCTCACGGTCTGGCTTCTAAGCCCTTACAACGCTCTCCTAACGCATTTCTGCCCGTAGCTTCGGTATTGTGTTTTAGTCCCGTTGAATTATCGGCACAAAGTCTCTCGACTAGTGAGCTATTACGCACTCTTTAAACGGTGGCTGCTTCTAAGCCAACATCCTAGCTGTTTAAGAAACTTCACAACCTTTCTCACTTAACACAATTTTGGGACCTTAGCTGACGATCTGGGTTGTTCCCCTCGCGTGCATCGACGTTATCACCGATGTACCGACTGCATAGTAATACATGATAGTATTCGGAGTTTGATTATAGTCAGTACGGCTAGGCGCCGCCATTCCATATTCAGTGCTCTACCCCCATCATTTAACACTACACGCTAGCCCTAAAGCTATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCACCCCTACCCACAAGTCATCCGGGCACTTTTTAGCGTACTGCGGTTCGGTCCTCCACTTAGTGTTACCTAAGTTTCAACCTGCTCATGGGTAGATCACCTGGTTTCGGGTCTATATCAACATACTAAATCGCCCTATTCAGACTCGATTTCTCTACGGCTTCGCTTTATTCTACTTAACCTCGCATGTTGACATAACTCGCCGGTCCATTCTGCAAGATGTACGCCATCACCCATTAACGGGCTCTGACTAACTGTAAGTAATTGGTTTCAGAATCTATTTCACTCCCCTCCCGGGGTTCTTTTCACCTTTCCCTCACGGTACTAGTTCACTATCGGTGTCTGGTTAGTATTTAGCCTTACCGGGTGGTCCCGGCAAATTCAGACAGGGTTTCACGTGCCCCGCCCTACTCAGGATACTGCTAGGAGATTTATACATTTCGCTTACGGGAATTTCACCCTCTATGTTTAAGCATTCCAACTTATTCTGCTATGTACAAATTCATTAATCCATGTCGCAGTCCTACAACCCCAACAACATGTGTTGGTTTGGGCTCTTCCCCGTTCGCTCGCCACTACTTAGGGAATCATTCTTTATTTTCTCTTCCTGCTGCTACTGAGATGTTTCAATTCACAGCGTGTCTCTTCATTCGACTATGAATTCATCGATATGATAATTGAGGATTAGCTCAATTAGGTTTCCCCATTCGGAAATCCCCGGATAACAGCTTATTTCCAGCTAACCGAGGCTTATCGCAGGTAATCACGTCCTTCATCGACTTCCAGACCCAAGGCATCCACCAAAAACTCTTGCTTGTTTAATAGTTTTGTTAATTGTATGACCTATTGTTTGTCTTTATAAAATATAAATATTCTATGGTTGATCTAAACTTTGAAATATTAATTAATATATCAATGTCGATATTCAGTTTTCAAAGAACCGAGAGATAAATCTCTCAAAACTGGATACAAATATTTTTTCCATAACGTAGGTTGTACTCCGTAGAAAGGAGGTGATCCATCCCCACGTTCTCGTAGGGATACCTTGTTACGACTTCACCCCAGTCACCAGTCCTACCTTAGGCGGTCGCCTCCGAGGTTAGCAAACCGACTTTGGGTATTACCAGCTCCCATGGTGTGACGGGCGGTGTGTACAAGACCCGAGAACGTATTCACCGCAGCATAGCTGATCTGCGATTACTAGCGATTCCGACTTCATGGAGTCGAATTGCAGACTCCAATCCGAACTGAGATCGGCTTTTTGAGATTTGCTCCATGTCGCCATATTGCTTCTCTTTGTACCGACCATTGTAGCACGTGTGTGGCCCCACTCGTAAGAGGCATGATGATTTGACGTCATCCCCACCTTCCTCCCAGTTACCCAGGCAGTATCTCTAGAGTCCTCAACTTAATGTTAGTAACTAAAGATAGGGGTTGCGCTCGTTGCAGGACTTGACCAAACATCTCACGACACGAGCTGACGACAACCATGCACCATCTGTCACTCCGATAACCTCCACTATATCTCTATAGCTTTGCGAAGGATGTCAAGAGTGGGTAAGGTTTTCCGTGTATCTTCAAATTAAACCACATGCTCCACCGCTTGTGCGGGTCCCCGTCAATTCCTTTAAGTTTCACTCTTGCGAGCATACTACTCAGGCGGATCATTTAATGCGTTAGCTGCGTCAGTGATTCTCCACCGACTAATGATCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTATAGACCCAGTAAGCTGCCTTCGCCTTTGGTGTTCTTCCATATATCTACGCATTTCACCGCTTCACATGGAATTCCGCTTACCTCTATCTAACTCTAGTTTGCTAGTATCCAAAGCGAGCCGGGGTTGAGCCCCGGGATTTAACTCCAGACTTAACAAACAGCCTACGAACGCTTTACGCCCAATAATTCCGGATAACGCTTGCGACCTATGTATTACCGCGGCTGCTGGCACATAGTTAGCCATCGCTTTCTGACAAGGTACCGTCAGTCTGCAATCATTTCCTATTGCAAATGTTCTTCCCTTATAACAGCACTTTACAATCCGAAGACCTTCATCGTGCACGCTGTGTCGCTCCATCAAGCTTTCGCTCATTGTGGAATATTCCCTACTGCTGCCTCCCGTAGGAGTTTGGGCCGTATCTCAGTCCCAATGTGGCCGTTCAGTCTCTCGACCCGGCTAAACATCATAGTCTTGGTGGGCCATTACCTCACCAACTAACTAATGTTCCGCACCCTCATCTTTTAGTGGCGCCTTACAGCGCCTTTCACAACGGAACCATGCGGTTCCATGCGTATCCGGCATTAGCCATTGTTTCCAATGGGTATTCCAATCTAAAAGGTAGATTAAGCACGTGTTACTCACCCATTCGCCGCTAGGTTATTGCTAACCTCGCTCGACATGCATGTATTAGGCACACAGCCAGCGTTCATCCTGAGCCAGGATCAAACTCTTATAAAAAAATTTGAATTGGTTGATTATAAATAAAAAAATAAATTGACGTTAATGGTATTCGTATCCAGTTTTCAAAGAACTATCTCGTTGTTTCAAAACAACCTTTAAATATTATATACACTTTTTTTTATTTTTTCAAAAAAAATTAAATTTTTTAGCGATATAAAATTGCTGTTTTAAAACAGAAAAGTAAATATGTATATATTATAACTGTTTTATTTTTTAAATAATAAAAAAATCAGGATTTTTTCCTGATTCTAATATCTATTCATTATTTTATTGTTAACCTACTATTTCTAGATCTGCATAATCAAATTCAGTTGGAACTTTTCTACCGAATTGTTCAATATTAACAAAGGCTTTTTGGGTAATATCATTATTTTTAATTATTTCGCCAACTTCATCTTTGAATATTCCGCTAGAAATTTTTACTAAAGCCCCTTCTTTAAAAGCAGTTTCAATGTCCCCTGCTTTAAATTTAGCTAGCAACATTGCTTCTTTATCAATCATTAATTGAAATTTTTTATCACTTATTGGGGTTGGTTTTGTTTTGTTACCACTTGAACCAATTAGCCCGGTTACATATTGAGTATTTCTAACAAGATATCATGTATCATCAGTCATTGCCATATTTAAGAAAATATAACCTTTGTACATATTTACAAATTTAACAGTAAATTCTTCGTTTCTTTGCTTCTTTTCTAATTCTTTATTTGATAAATGGGGTTCTTTAAACATTCTTATATCTTTAAAGTATTCGTCCATTCTTTGAGCAGCAATTTTATTTTTTAATGATTCTATAACATTTTCTTCTTTTCCTGAAACTGTTGAAATCATATATCATTTAAAATCATTATTTGTTAATTCCATATTCTTTCCTTACTAAGCCTTTATTCCTACTGAATTTCATAAACTACTAAATCCAAGGGTTATTGCAAAACAAAATGCAGCCATTAAAACTAGAAATAAAATTGTAATACCAAATCATTTTCAAGATTTTGCAGATTTTGGCCAGATAACTCTCTTTATTTCCTTAATTCAATTTCGAAATGTATATAGCTTAACTTGGCCTTTTTCTGCTTTTGCTTGCAATTTAGCTTGTTTTCTTTGTTCTCTAGTCATTATTTTTCCTCTTTGTGCAATGTTTGTTGATTACAAAATTTGCAAAATTTATTAATTTCTAATCTCTTTTCATTGCTTTTATTTACCGAGTAATTTTTATGCAAACATACTTGGCAGGCCAATGTAACCTTCTTTGACTTTGTATTCATAATTAGATATTATAATATATTAATTTCAAATTTTCAATCATTTTTAATAGTAATTTATATTAAATAATTGTTGCGTAAGAAATTTTATATAATTTTAAAATATGAGTAAATGAGCAATTTTGTTTTTAGTAATCTTAATTATGCTAATTGCAATGTATTTAACATTTATTTTTTATTATTTTATAAAAATATATCCCCAAGTTTTGAAATCAAAATTGGATAATTTTATTAATAAAATTTGAACATTTATTAATAAAGCAAAAAATTCTTATGACAAAATTTGCCAATTAAGTTTGAACAATGATGAATGCAAGGAACTAGAAAAACAAATTGGAATTAATTTGCATGAAGTTTGTAGTTTATATAAGGCATGCAAAATATTAAAAACCAAAGTAGACGATATTGTTTATTTGCATATGAATAAGCATACTTTTTGACACTTAAAATGTACAATTCTTATTAAAAAGAAATATAAAGAAATTTATTTTAAAAGACTAGAAATTTTTAATTTACTTGAAACTATCTTTAAAAAAGTCGCAGAAATTGATAAAAAAAGTGGAAATATACAAAAAATAGTAAATAAATACAAAGTTAGTTTAAATGATTTGTCAAATATAATGGCAGGTGAACATGAACATTTTCCTCAACACTTTATTGATAATTTAAAAGACAAAAAAACAATTATACAAAAAAAATTAGTTGATTTTAATAATGATGAATATCTAAAATGAAACGAAAAAATATTAAAGCAAAATTTATTAGAAATTAAAAATTTAATCATTGAATATGCAACCTTTTATTTTAAATTCAGAAACAATTACAAGAATTTAAATTATATCGTTGAAAAATACAAAAAAAATCAACAGCTTATTAAAGAATTAAATAATATTAAATTAGAGCAATTAATAAAAGAAATGTTGGAAAAGAAATTTAAATTTAATTATGAAATAGAAGTTGAAAAAATAAAAAGTTTGATTAACAAATGTCAAAATTCAAAAGACGTTTCCAATATTGAACAAATTATTAATAAACTATTGGCTTCAATAAATACAACTAGTATAAAAATCTATAACTATATTAAAACCTTTGCCTTTCATTTAAAAATACAAAATAATATAAAAAAAATCATTGAAAAAATTGATATTCAAAATAAAAAAATTTCTGATTTTTTTGCAAGTGAAACTAAAAAGAATTTATTAAAAAGTGAATACCTTAATACTTATAAGCCAATATTGAATAGTTTTATAGATATTAAAAATAATAATTGAATGGATATAGTTGAATTTACAAAGTTGAAGAGTTGATTAGAATCTTTATTGAATTTGCTAAGGGTTCAAAATAAAATAATAAATAAAATAGATGAACAAGAATATCGAAACAATCAAATTGAATTCTATATTGAAAACTTTAAAAGATTAACTGCCAATATAAAAGTATTAAATATTAGTTTAAATCCAATAGACATTGAAAACATAAATAGTTTATTTACTTTTATTTATAAATTCAAAAGTAATGGTTTTTTTGCAAATGAATTGAATAATTTTTTAAGTTTTTTATCTAATGAAATAAATTTTTTAAGTTGCAAAATTCAATATTTTTTAATAATTAAAAATGTAGAATTTTCTTTAAGAAAAGACGTAAGTGAAAATTCAAATGCCCAAAATTTATTTAATGAGTTGCAAAAACTTAAAGGTTACAATGATGATAAAAAAGCAATTGATTTAATATATGATTATATAGAAGAACAATACAACTAATGGAGTAAACATGGACTACAATAAAATTTTAATTAGATATGGTGAATTGACTCTTAAGGGAGAAAATAAAAAAGATTTTATTAGACAATTGCATAACAACCTATCTTGATATATAGAAAACTCAGAAATTAAAACCGAATATGACCGAGCATTTGTTGATTATTCGCAAAAAAATTTAGACTGCTTAAAATATATTTTTGGAATTTCTTCATATTCGCCCGTTTATGAAACAGAAACCAACCTAATCAATATTGAAAATATAATAAAAGAAATAATTAGTAAAAAACAATTTAATAGTTTTGCAATAAATGCTCGAAGACACAACAAGAATTTTTCAATGACTTCAAGTGAACTAAATATGTATTTTGGAGGTATTGTTTTAAAACAAATGCCAGATAAAGAAGTTAAATTAAAAAATCCTGATTTAACTATATATATTGAAATAAGAGATCAACGTAGCTTTGTTTTTTGCGATTATTTTGATGGCTTAGGTGGTATGCCGGTATTGAGTTCTGGAAAATGCATACATCTAATTAGTGGGGGCATTGACTCGCCTGTAGCAGCAAACTTATTACAAAAAAGAGGTTTAAAAATAGTGTTTTTAAATTTTATTACCCCTCCTCAAACAGATGAAAAAACAATTGCAAAAATAGATGAAATTGTTAATATACTAAAAAAATATCAAGGCAATGCTATGTTATATCAAATTAACTATAGCAAAGTAATGGAATATATTGGCCTTATAAGTAATCAAAAATACAAAATAACTTTAATGAGAAGATCTTTTTATAGAATAGCAAATCTTATTGCAAAAAAAGAAAACATTAAAGCAATTTCAAATGGTGAAAATTTAGCCCAAGTTGCATCTCAAACTCTTGAATCTATTTATACAATCTCCGAAGTTTGTGATTTGCCTATTTTTAGACCTTTAATTTCTTTTGACAAAAATGAAACTATTGCTATTGCAAAAAAAATTAGCACATTAGATGTTTCAATAGTAAGGGCGTGTGAAACATGTGAATTATTTGCTCCTAAAAATCCTGTAACCAAGCCTAATAGGCAAGAAGCTCATAAACTCGAAGAAGAAGAATTGCCTTTGATAAAAGAATTTGAAGAAGAATGTATTAAAAATGCTATTATAAAAAAATATTCAATATAATCGTTTTAAATGTTTAATAATAAAAAAGGTTTGATTGAACAAACCTTTTTTATTATTTTAAATCTTTTAATTGAGGGAATAAAACTACATCTCTAATTGAATCATTTTGAGTTAATAGCATTATTAAACGATCAATTCCTATACCAGAACCGCCTGTTGGAGGCATTCCATATTCCAAAGCATTAACAAAATCTCAATCAATTTCATTTGCTTCTTCGTTACCGTTTTCTTTTTCCAACAATTGGTTTTCAAAACGTTGCAATTGGTCAATTGGATCATTTAATTCAGTAAACATATTTGCAAATTCTTTTGTTCCAATAAATAGTTCAGCACGTAGTGTAAATCTTTTATCGTCAGGGTCAATTGCTGCAAGAGGAGAAATTTCAATTGGGTGGCCATATACAAATGTTGGTTCTATTAATGTTTCTTCAACATATTTTTCAAATAACTCATTGATGATGTGACCATATCTAAAGAATTTTTCAACTTTAATACCATGTTTTTTAGCTAATTCAAACATTTGTTCTTCATTTAATTTTCTAATGTCAACATTAATTTTTTCCGATATAGCATCAATCATATTGACTCTCTTAAATGGAAAATTAAATGTTATATCAAAGCCATTAAATTTAACTTCTTTAATATTGAATTTATTTATTAAATGTTTGAAAACACCTTCAGTTCTTTCCATCATTCCTCATAAATCTGAATATGCTTCATAAAATTCAATTGAGGTAAATTCTGGATTATGAGTTGTATCTACGCCTTCATTTCTAAATATTCTACCGATTTCATAAACTCTATCTAATCCACCAACAATCAATTTTTTTAATGGCAATTCAGTTGCAATTCTTAAATAAAAGTTTCTATTAAGTGCATTATAGAAAGTAATAAAAGGTTTAGCAGCTGCTCCTCCTAATATTGGATTTAAAATTGGTGTATCAACTTCAATGTAGTTCAAACTATCAAAATATTCTCTAATGCCTTTGATAATTTGGGTTCTCATAACAAATGTATTTCTTGTTTTTTCATTCATTATTAAATCTACATAGCGTTGACGACGTCTTTCTTCTGGATCAACTAAACCATGAAATTTATCAGGTAAGGGTTTCAATGATTTTGTTAATAGAACTATGTCTTGAGCTTTGATAACCAAAGCATCAGTATTAGTTTTTGATAATGTTCCTCTTACACTTAAAATATCGCCAAAATCCATTAAAGAAAAGGTTTTTGTAGAAGACTCATTCAATGTTTTTTTGTTTGCATAAATTTGCATTTGTCCGCAATTATCTTGTAAAACAATAAATGGGCCACGAATAGTCATAATCCTTCCATTAAATACAAATTCTAAATTTTGTTTGTCAAGATCTTCACGGCTTAAATTTTTATATTTTTCATTTAACTCTTTTGAATTTGTTGTTGGTTTTATAGTTTTGTGAAATGCTTGTAAATTGTTTTTTTCTAATTCAGCAATTTTATTACGTCTAATTTCTTCTTGTTCACTAAGTTTTCTTTCCATAAATATTTATCACTTTCTATTTCTTAAAATATCAAAAAAATTATATATTATATGCTTTATTTTTTTTATAATTATTAATGCATAATTGCGTGGTCGCATAGCTCAGTGGAAGAGCACGAGCCTCCTAAGCCCGGGGTCGCAGGTTCAACTCCTGTTGCGATCGCCATTTTTTTATACATTTTAATTAATTAAAAATAGTCCAAAAGGACTATTGGGCATTCTTCATAGAACGCATAACTTGTCTAATTTGTGCTTCCGAAGCTTTTCTTCCCATTTGTTGAAACATTACACGAATCATTTTTTCAGTAACTGGTGGATTTTCTTTTAATTGCTTCTTGAATTTTTTTTGGGTTATATATACCGCAATTATTCCACCGATTATGGCAGTAATTATTGGTAATATAACTAATAACGCTATTCCTCAACCTTGCATATTCTACTTTCCTTTCAATACTGTCTTCTTTAAAATAATATATATTATAAAGGGCAATACAATTAAAACTGCGATACTTATAAAGAAAATTTTGGCATAAAATTTGTGAGCTTTTCTTTTGCTTTCATCAAGTTCAACAAAATTCATTCCTTGTTCATTGCCAAAGAATGATTGGATGTCCACCATTCTTTTCTTTTTAAAAATTGTACTGAATATAAATAATATTAAAACTATTCCAATTATACCACATAGTATGTAAATTAGTATTTCGGGTTTTGGAAAAGTATTATGTATTCATTTTTGTCAATCCAATAAACCCCATTTTGCATCTTTTAAACCTCAAAAAATACCTATAAACACACCAATATAAAACACAATAGCAACAACTGCTCATGTTTGTCTTACTTGTCTTATCATTAATTTCTTATAAAGTAAGGCAATAAATGGCGGTGTTGATGTCGCACCATTTTTAATGGTAATTCTATACATATCTATGCTTTTATGTATTCCTGAAAGTTCAATCAATTCAATAGTAAATCTAGTTAACGAAATTACTATAAATATTGCTGGAATAATATATCAAGCCCATTTTATTTTAAAATCTTTTGCATTAGAAAAATTGAATAAATTTGGTCAAAAGTAAAGCAATAATATTGCTATAACACTCAAAAGAAGGATACTAAAATTTCAAAGTATTGATATTTTTTTAATATTCATTTCTCTTTGAAAGACTGAATAAATTCCATTAGGAATTATTTTCTGAGGATCTTTTATTTTGTAACTTATTTGTTGCAAATTTTGAGTATTCATTGAATCTTCTACCATGTGATTTTCAAAGGTACGTGTTGGAATTTCAATGTTTTTACTACTATCGCTATTATTTCCTGTTGTTGTGGGCTTAATATCCATTATTTTCTAATCCTTTCGAGAATCATCTGTTTTTAATATTGCCAAAAATGCTTCTTGTGGAACTTCAACGCTTCCTATTTGTTTCATCCTTTTTTTACCTATTTTTTGTTTTTTTAATAGTTTTTGTCTTCTTGTAACGTCACCACCATATAACTTAGCTGTAACATCTTTTCTATATGCCTTAATTGTTTCTCTTGCTATAATTTTAGCACCAATTGCAGCTTGAACGGGAATTTCAAAATTTTGTCTTGGTACAACTTCCTTTAATTTTTCTGTTAATGCTCTACTTTTATTATATGCCGAGTCTTTATGGACAATCATTGCTAAGGCATCAATTTTCTCACCATTTAATAAAATATCAACCTTTACTAAATCGCTTTCTCTTAAACCAATGTAGTCATAATCAAATGAAGCATAACCTTTTGAATAGCTCTTCATTAAATCAAAGAAATCAAATATTATTTCATTTAAAGGCAATTCATAAATCAATAATCTGCGAGTTTCATCTAAATATTCAATATTTACATATTTACCACGCTTAGATTGGCATAATTCCATAATTGGGCCCAAATATTCTTCGGTTAACATTATATTAGCCTTAATATAAGGTTCCTCTATCATTTTTATATAACTTCTGTCTGGAAATAGTGAAGGATTTGTAATATATTGAACGTCCCCATTGGTTAGTGTTACAACAAACTCAACTGAAGGAGCAGTAGCAATAATATCTAAATTAAATTCTCTTTCGAGTCTTTCTTGAAGAATATCCATATGCAACAAACCTAGAAAACCTATTCTAAATCCGAATCCTAAGGCTTTTGATGTCTCTGGTTGTCATACCAATGAAGAATCAGATAAACTAATTTTTTCTAGGGCATCTTTTAAATCATTGTAATCTCTAGTATCGACAGGATAAAATCCAGTATAAACAACTGGTTTTATTTTTTTATATCCTGGCAATGGTTCAGGGCATTGATTTTCAAATAACGTGACAGTATCACCGACTTCAACATCTTTGATATCCCTTATTGATGCTGAAATTCAACCAACTTCGCCAGCTTGCAATTCATTTTTTTGAACTTCATTAGGATTTTTAACGCCTAGTTCCGTTACTAAATAATGCTTTTCTGTTTGCATAAAGTAAATTTCGTCGCCTACTTTTACTTTGCCATCAAACAATCTCACATAGATAACAACGCCACGATAATTGTCAAAATAGCTATCAAAAATTAAAGCTTGCAATGGTTTATTTATATCTGCTTCTTTAGGAGCGGGGATTTTGTTTATAATTGCATCAATTACTTTATCAATATTCAATCCTGTCTTTGCTGAAATCATAATTGCATCAGAAGCATCAATTCCAATCATGTCTTGAATTTCTTTTTTTACTCTTTCGGGATCTGCACTAATTAAATCTACCTTATTAATAATAGGAACGATTTCTAAATTATTTTCCATTGCCATATAAACATTAGCCAATGTTTGTGCTTGAATTCCTTGAGTTGCATCAACTAGCAATAATGCTCCTTCACATGCAGCAAGTGACCTACTAACCTCATAAGTAAAATCAACATGGCCTGGTGTATCTATCAGGTGAAAAATGTAATCTTTATATTTAATTTGAATAGCATTTAATTTGATGGTGATTCCACGTTCTTGTTCTAGTTCCATTGAATCTAAAATTTGAGGTTTTGCTTCCCTGGCAGAAATTGTATTGGTAAATTCCAAAATACGATCAGCTAAAGTACTTTTACCGTGATCAATGTGTGCTATTATTGCAAAATTTCTTATTTTTTCGTTTGTCATATTATTTTAAATTATATTTAAAAATGTCATACATTTAAAGAAAAATAAAAAATAACCATAAATTAGATATATTTATGAAATATCTCTATGGTTATTTTTTTAATTATTTTTTGTTTCCTTGGCGCCAAATATTTGTTCAAATATTTCGTCATAATAATGAACTGGTACAAATTCAACAACATTTTTAACTTCAACAGGAACATCTTTTAGATTTTTATAATTATCATATGGAATAAAAATCTTTTTAATACCAAATTGAGCAGCTGCCAATGATTTTTCTTTTAAACCGCCAATTGGTAGAACTTTTCCGCGCAATGTAATTTCTCCAGTCATACCGATATTAGCAGGGACTGGTCTTTTAGATAAGGCACTAATTATTGAGGTTGTGAATGTTACTCCGGCACTTGGGCCATCTTTAGGAATAGCACCTGCTGGAACGTGAATATGAATTGCTGTATTTTCAAAATCAAAGTCAATATTAAATTTGGCAGCATTTGCTCTTACATAAGCTAATGCAATTTGAGCAGATTCACGCATAACATCTTTTAGTTGCCCTGTCATTTTTAAACCTTCTTTTCCAGGGAATGTTGTAACTTCAATAGCTAATGTTGAACCACCAAAACTTGTATATGCCAAACCATTAACAACACCTATTTGTGCCTTATTTTCATTTTCATCATCATCGAATTTAATTGGTCCTAAAAATTCAATTACAACTTCTTTTGTAACAACAAAACTATCGTTGATTTTTTTGTCAAGAATTTCGACAACAATCTTTCTTGCTATACTATCTAAAATACGTTTTAATTCACGAACTCCACTTTCCCTTGTGTAGTAACGAACTAAAAATTCTAAAACTTCGTCAGAAATTTGAAATTGTTCAGGATTTAAGGCATTTTGTTCATACACTTTTGGAAGTAAATGCGATTTAGCAATTTCTACTTTTTCTTTAGTTGTATATGTTGATAGTTCAAGTAATTCAACACGATCAATTAGTGCTGCGGGTATTGAATCATAGTAGTTAGCAGTAGCGATGAATAATACTTTTGATAAGTCATATTCTAAATCCAAGTAGTGATCTTGAAAATTAACATTTTGTTCAGGGTCTAATACTTCAAGCATTGCGCTTGTTGGATCGCCTCGAAAATCTGATGACATCTTATCAATTTCATCTAATAAAATGACTGGGTTTGAAACTCCGGCTTTTTTAATTGCTGATATTATTTTTCCAGGCATTGCCCCAACATAAGTTCTTCTGTGTCCTCTTATTTCACTTTCATCCTTAACACCGCCCAATGAAACCTTTATAAATCTACGATTTAATGATTCGGCGATTGATTTAGCAAGTGTAGTTTTACCAGTTCCTGGAGGACCGATTAATGTTAAAATAGGAATATTATTTACTGGTTTGTTTGTGGTTTGACCTGTTTTATGAACGAATAATTTAGTATCAATAAATTCTTGATCATTTCCTTTTATTGGTAAATATTCAGTTTTTTCTTCCTTGATTTTAAGATGAGTTAAAACCGAAATAAATTCAAGAATTCTTTCCTTTGGCTTTTCAAGTCCATAGTGATTTTTATTTAATACTTCTCTAACTTTGTTAATATCAATTAATTCTTTACTTACTTTTCTTCAAGGCAATGCAAGGATCAAATCGATATATGTTTTAGATATATTAGCTTCTGGGCTAGATGGCATCATTGAAGCCATTCTTGATTGTTCAGATCTTAATGCTTCTAAAACATATTCAGGATATTGTTCTTTTAAAGTACTGTCTTTTTCAATGTCGTCAGCAGATTTTCCACCTTCATCATCCTTCAACATTTTTCTAATTTGACGCATTTTTTCTCTTAGGAAGAAATCTCTTTGTTGGTCTTCCATATCTTCACGTAAATTACTATTAATTTCGTTTTCTATTTTTTCAAATTGAACTTGTTTATTTGCTTTTTTAATTAGTTCATTGTATCTAGCTAAAACATCATTACTTTCAATTAAAGAAAGAAGTTCATCACTCAAAAAGTACTTCTTCAAAAACTTTATAAGTTGTCATGATTCAATTTCGTATTCAACTAATGATTGTTTAGGTTTGTTAGTTTCAAATTCTTTTTCTAAATCTCGAAATAAAACTGTTCCTACGCCTTCATTAATAACTTGTTTAGATAGCAAGAAGAACTTTTGTCTTATTTCTTCAATTTTTGGTAAAAATGAATCATATGAATTGGCTTGAATAGGCGAAATATTAACATAAAAATTATCCTCACTATTTGATGATTTTTCATCAACTATTGAGATTTCATTAATATTAACAGGTTCTTTTGCTTCTAAATTAACATGATAAATTCCTGATTCTTCACTAATGTTTGTAATATTAGCAAGCACACCTACAGGAGCAATATCATTAATTGTATGTTCCTCATTTGATTGTTTATAATTTGGATTTAAAAATGTAATAACTATATTTTTATTTCCATTAAAAGCCTTTAATAAAGCTGATACTGATTTAGCTTTTCCAACTCTAATTTTGTCAGAAGTATATGGAATAATAATCTGATTTTTTATTGCTATATAAGGTAATTTCATTGTTATCTCCTTAGAAAAATGAATTAATTATTTAATATTTTATATTAAATGTTGTTTTTTATTAGTTTATTTGCTAATTATTACTGAGGCAGGTTTTAAAATTCTGTTAAATAATGAATAACCATTGCTCTTTACTTCAAGAATTGTGCCAGAAGGTTCTTTTGATTCAATGCTTTCAGTAATTTGTTGACATTCAGGGTCGAATAAATCTCCTACTTTTGGTTCAATTATTTTTATTCCGTTATCTAACATTAAATTTTCAAAATCATTCTTAAGCATATCAAAGCCTTTTGCAAAATTATTAACAGCGATATTATCAGATTTTTTTGCAAAATTTAAAGCACTGTCATATTTTGTATATATCTTAATGAACTCTTCTAAAAAGTCTTGTAGGGCATATTTTTTTAATTCTGTTTTTTGTTGCAATAAATGCTCATCATTTTTTTGGCGATGTTCATTTATTTTTGATTGTGCTTTTGCTTCCAATTCGATTACTTGATTTTTAAACAAAATTTCTTGTTGTGTCTTTTCTTGTTTTATTTTTTCATTAAGTTCGCCTAATTCCAAAAAATTTAGTTGTAATTTTTTAATATTTTCATTCAATTCTTGATTTTTAGCAAAAACTTCTAAATACTTAGGACTTGTTTTTTGAAAATCAACAATTATTACCTTAAAAATTTCATTATCGATTTTTAAAACTAACTCACCTTTTTCGTTTTGCTTTCCTTCCAAGATAGTTTTTTCAATATTTTTATCTAAATCAAAGCCAACAAATCCCTTATATTCAATTTTTTCATCTTTGCTAATTTTTCTAATGCACTCTATATAATCATATTTTGAAATATTTTGACTCATTTATTCTCCTTTTTCTTCTTCTTTTTTAATAATACCTTCAAATAAATTTATCGCTTCTAATGATTCTTCAACATCTAAATTTTTAGGGCCTAATATACTTATTTCTTTAATATTTTTGTGATTAATAAATTGAATTCTTTTTGAAATAATATTTAAATTAGGATGTGAAAAATCTAGTTTTATGTTTTTTTCTTCATCAAGATTGTTCTCAATTGCTTCTCAAACTGAGTGGTTTTCAATTAATTCTAATGTTTTAGCGATTTCTTCTCTGCTTATTTGTTCTGATAATATTATTACATTTTTATTAAATGATTTATTCTCCAATTTTTCTTCAAAACAAAAGATATTTTTGATAAATTTCTGCATTATTAATTCAAAATGTTTAACTTGTGTAGATAATATTGGGGCTAATGATTCAGTTTTTTTAGCAAGATTAATTAATGGAGTATCAATCAATCTTTCTTTAAATAACCTAATTGCAATCCTTAAATCATTTAATTCAATCCCTCTTTCAAACTTAAACATTTTGTTTTGAACATTTCCCGAGCTTGTAACAATAACAACAACTGCAGATTCTTCATCAAGCGGTGTCAATTGAATACTTTTCAATAATTCAGATTCATTATTTGAGGTTGCAACTACGGCAAAACCTGCCATTTGGCTAACAACGTTTGCTGCTTCTTCTAATGTTGTGTTGATGCTTAACCTACGCTTAGATAAAAGATCTTCAAGTTTTTCATTGAAATATTTTTTAGCATTATAAAGTAAATTTTTTGTATAGTATTCAAGACCTTTTGAAGAAGGAATTCTTCCTCCTGAAACATGTCTTTTTTCCAAAAGACCTAATTTTTCTAATTCTGCCATTGCGTTTCTTATGGTTGCAGAGCTACAAGATAATTTGAACTTTTCAACTAGTCTCTTTGATCCTACTGGTTCACCTGTTTTAATGTACATTTCAACAATGTATCTAAAATATTTAATTAGTTTTTTATCATAATTATTGCATATGATTAAAGACTTATTTGTGTTTGTGTCAGAAGAATTTTGCTCCATGATTGCCTCTTTCAAAAAAATTTTAGCACATTTTAGCACTTTAATGCGTTAATTGCTAATTTTTTGAAAATTAATAGTTTAAAAAAACTAGGGTTTAACCTAGTTTTTAATTCAGTTTAACTGATATGGCGCGCCCGGCAGGAGTCGAACCCGCAACCTTTTGGGCCGTAACCAAACACTCTGTCCAATTGAGCTACGGGCGCATTGGAGCCACCAACCGGATTCGAACCGGTAATCAAGGTGTTGCAGACCTATGCCTTGGCCGTTTGGCTATGGTGGCAACACTGGCATGCATGTTTGCATATAGAATTATAATACATTTTATAAATTTTTAAAACAAAATTAGTAAAGAAAAAAGCGCAAGAGGGGACGCTTTAATACTATATATTTTCTTTAAATATGCAATAATTATATTATTTTTTTTAAAAAAATCGTTTTTTATTATTTTTAAATGCTTTATTTTAAACAAAAATTAATAATTTTAGAACGAAATGAACTTTTTCATTAATCTTAAGACTGTCAATTTAAGCAATGATTCAATCCGAGTTATGCCATCATTACCAGGCATTTTATTAAATATTATATGTTGCTTCGAAGAAGTAAAATCTATAGGTGAGCTCATAAAAGTGATTTTATTTTTTTGTTCACGTTCGCTCAATATATTAAATATGTTTGTAATATACCATTCCGGTTTGACTCCGATTGCAAAGCGATCTATGAATAAATAATCAACAGAAATTAAGTTTTGAACAATTGAATTAATTTCTGATGAGTTTTTTAAATTATTAGACATGAATTCAAACAAATTATTGTTGTCAACAAATGCTACGCTTTTGTTTTTCTTAGTGCCAAATAAAAATGCTAAAAATTTTAAAATATTTCTTGCATTAACAAGATTTTGATCAATAAGCAATAATCCCTGATTTGATTTCATCGAAGTAATATCTTTGATTTCATTTTTTAATTCAGGCGCAAAATTTTCTAATGATTGGTTTGTGTTCAATACTATATATTGAGGTTTCTTTGCTTTTGCATTATTAAAATAATAAATTCATTCATTATCCATGCTTGTTATATTTGTTAGTCAAAAGTTATTCATTTCTTTTTGCTTTCTAAAAAAACCATCGTTGCTCAAATCAATTTGCAAAATTCCACTATCATCAACAAAGAGGGTCCATGTTGGAAGTTGTTTATGCATTTCAATATAGCTATAATATTTTTGCAAGTAATTCATTCCTTTTTGAATTTGTTTAGAATTCAAATTCAATTTTTCAATAATTGCTTTTACATTTGGATCGGCTAGAATTTTTGTTTTTTCAGCCTCTAAATCAATATTTTTCAAAGGAGAATTTTTAAACAATTCATCAAATATTTTGTTTTCCATTTTTTTGCTTTCTTATTTTTTAGTTAGTTCAATCGTGTTCATTAGCAAGATCTAAAAAACAATTTAAATCTTCTCTAGTAACCGATTCATCTTCATTTTTTTTGATATTTTTTAACTCTTTATTTGCGTTTTGCTTTATTAAATAAACATTTTCTAATTCTTGATCAATTCTTTCGGCATCGTATAGATTTCTATGACTAAAATCTTCGGCTATTTTTTCAATATATTTGCATACAACTGAATGGTTAATTTTAATTGAATAATAAATGAAGGCATTTATTGAAAAATTATTGAATTTCATTCTTTTCAATTTTCTAATCATTTCATTTTGGCTAAAACTTAAATTTGTTTTTGTATATTGACTAATAAATTGTTCGGGTTCTATCTTATCCTTTAATTCATCTAGGGTAATTGAATTAAAATTTAATTGTTCATTTTTTATATTTAAATCAATTACGTTTTCTAAATTAAATACTTCATAAAATTTTTTAGAAACATCATTAATTGTTGTTTTATCAAATTGATAATTTGCATTTAATTTTAATAATTCTTCATATTTTGATTTAGTGATTAATTTAATTAACATATTAGAAATAATTTTATTATTGCTTATTTCATTTGCATTCAATGGAGCATTTAAACTAAAAATATAAACACCATCTTTATTTAAATAACTTTTTAGTAAGCTTAATGCTTCTAGTTTGCTACGACATTCTTCAAATTCCTGTTCATTTAATAATAAAATATCTTTAATTTCAGAAAAATTAAAAAGTTTATTTTTTGCATAGATATTTTTTAAATTATATAAAAATTGATATAGAAATATGCTACTATGGCCTAAAATCGGAGCATAAAAAATTACTAGATTTTTAAAATCATTTTGTGTAATTTCCCTTTCGCTTTCAATAAAAAAATTTTCAACCATATTTAGCCCCTTTCTAATTAGTCGTGTACAAATATGATAAATTGCTTAGAAAATAAAAATTAATTTTTCTTAGGTTTTTTAACAATTTAACTACTTATTAAAAAGTTATTAACAATGCACCGCATGCCTTTTAGCCTTAAAACTAGATACTTTTTAAACTATTTTTATTTTCTAACAGCAACAAGTGATTTTAAATGAATTTTCAGATTAAAAAAATGTAATAAAAAATAGAAAAATTTTTTTCTTGAAAATAGCTTTCGCTATGTTTTCTTATAGTTTCAAAAAATGTTTTATTTAATAATATAATTGAAATATGAAATTAATTAACCTTCACTTAAACACTATATTTTCTTTTTTAGAAAGCACGATAACCATTAATGATTTTATTCAAGAATTATTAAAGAATAATATAAAATATTTTGGCATTACAGAACATTGTAATTTTTATAGCATGCCATATTTTTTAAATGAAGCTAGAAAATATGATTTAAAACCTATTTTTGGTCTTGATGTAAATGTAAAAATAGAAAATAATTTGTATAGATTTATAGTTTACTGCCAAAATAAAGAATCTTTTATGAACCTAAAAATGCTTTCTTTAAACTACGGAAAAAAGGGCTATATAGATTTAGATGAAATTAGAGATTATTCTAATTTAATATGAATAGAACATCCAATTTTTGGCTATTATAAAAAAACTAATAATATTTTAGAAATTCAAAACCATTATTTTGCAATAAGCCAATACGATGTAGAAAATAGTGAAATACAAAATATTTTTGATAAGTGTTTATTAATTAATCACAATTCAATATTAAAATATGATGATAATTCAATAATTAAAACAATGTCGAATATTCCAAATAATAAAATATTTGAAAGCATTTACGAAGAATTTAAGGTTGATATTGAAACAAAAAATAATTCAAAATTAGAAGAATTAATTGAAAGAACAAATAATTTTGCTAAAAAACTTTATTTTGAAATTGAAACTAATGATTTTAAAATGCCTAAATTTAAAAACGACAAAAATATTGCTTCTAATGAATATTTAAAATTGTTATTATTTAAATCAATAAGAGAAAAATTCGATAAGTTTTCATGAACAGATGAATACCAACAAAGGTTTTTAAAAGAACTAAAAGTTATTGAAGATCTTAATTTTGCTGATTACTTTTTAGTTGTTCAAGATTGAGTTAATTGAGCAAAAAATAACAATATTAAAATAGGACCAGGAAGAGGTTCAGCAGCCGGTTCATTAATAGGCTATCTATTAAATATAACCGAAGTAGACCCTATTAAATATGATTTGATATTTGAAAGGTTCCTAAATTCTAATAGAGTTACTATGCCTGACATTGATATTGATGTTCAAGATAATAAAAGAAATGATGTTATTGAATATTTATTAAAAAAATATGGGCATGAATTCGTGGCAAATATTGTTACTTTTTCTTCACTAGGTAAAAAATCAGCGATTAGAGATATTTTAAGAATAAACAATATTGCCCCAACATTAATTAATGAAATATCAAAAAATATTGAAAATGATGAACAAGACCTTTTGGTTGAATACAATGAAAATAGAAATTTACAATTATCATTAGCAAAATTAAATCCTAATGATGAATCATTTAGCACAAGAATATTAAACCAAGCAGCAAGGATATCAGGATTTTATAGACAAATTGGCACACATGCTGCCGGATTAGTAATTAGCGACCAACCCGTCATCTCGATAGCACCAGTTCAATATAATTCTGAAAATTTTCTTCAAACTCAAATAAGCATGGAATATTTAGAAGATTTCGGGTTAGTTAAAATGGACATACTTGGTTTGAAGACATTGACGACTATTTCAAATATAATTGATCTAATTTATCAAAATCAAAATCTTAAAATTAACTTAAAAGATATAGACTTAACCGATCAAAAAACATTAGAATTATTGAGCTCTGGTCACACAAGAGGCGTTTTCCAAGTTGAAAGTCCAATCATGATAAAAGCCTTAAGGCAGGTAAAGGTAAATAATTTTGAAGATATTGTTGCAATAATTTCATTAAATAGACCGGGTCCAATGAAAAATGTTTCTGTTTATGCAAAAAGAAAATTTGGACTAGAATCGATACCAAAAATAAATAATGAATATGATAAAATCCTTGATTCTACTTATGGAATCATTGTATATCAAGAACAAATCATGAAAATTGCTCAAGTTGTTGCTAAAATGACATTCATTGAAGCGGACAATCTAAGAAGGATTATTTCTAAGAAAAAAGTAAATGAAATGGAAGATATTAAATCCATTTTTATTGAAAGAGCAATGAAAAATGGCTATGAAAAATCTTTGGCTACAAATATCTTTAACAGCATAGAAAAATTTGCATCATATGGATTCAATAGATCTCATGCGGTTTCATATTCAATTATAACTTTTCAAATGGCCTTTTTAAAAGCTCATTACCCTTTAGAATTTTATACGTCATGTATAAGCTCGGCACACGGCTCCCAAGATACAATTGCAAAGTTCGTTAACGAGGCAAGTGAGTGTGGGGTAGAAATAGTTTCTCCTGACATTAACCTTTCTGAATCAGAGGCTATAATTAAAAATAAAAAAATTATATTGCCGCTTACACTAATTAAAGGCCTTGGACCTGAAATTGCTAATTCTATTGTTGAAAATAGATCAAAATATGGCCCATATAAAAATTTCCTAGATTTCTTATTAAAAATTTCTAGCTCTCAATCAATTGGCAAAAGTACAATTGAATTATTAATAAAGGCAAACACTCTTAGATCATTTAATTATAATCAAAACACCTTTTTGAATGAGATTCAAAAAAATAATACTGATACAATGTTATTTATAAAAATGTTTAAAAATAACATTGAAGGTGTTAGCCCTGATATAATAAACTCTTATAAACCAAGTGAAATTCTTAATCAAGATATCATGTTTGAAAAGAAAAACGAAATTGAATTATTAGGTCAAGTTTATAATTTTAATTCAAACGCAAGCAATGGAATGCCTGGACAAACTAGTTTTATAGACATGCACGTTGGAAATGAATATTTAGCAATCGTTAACTGTACAAACGTAAAGCAAAAAATTTCTAAAAAAGGTAATAACTATTTTGAATTAGATTTAGAAGCAAACATGCAAAAAATAAGAGCATTTTATTTTACATCAAATCAAGAAATACTAAATATTAAGGGAAGAACTATCAAATTAACTCTAGAAAAAAGAAATGAAAATATTTTTTATTTGCGTTCAATAAAGGAAGTTTTAAATGAAGAAAGATAATGAACAACTGTTGATTATAGATGGTACATTTTTAGCATACAGATCCTATTATGCAACATCTTATAACGCAACTCAAATTTTAACCACATCAGAAGGATTTGAAACCAATGCGATAGTAGCATTCTTTAAAACACTTTTTTTATTACTAAAAAAATTAAATCCTAAATATATCTATATAGCCTTTGATTCGCATGTTAAGACGTTTAGGCATGAATTATTTCCTTCTTATAAAGATGGTAGAAAAAAAGCGCCAGAAAGTTTTTATCTACAGCTTGATTTAATTCAAGAAATATTAACAAATATTAATATTCAAAACCAATATTATAATGGCTTTGAAGCTGATGATATAATTGCAAAAGTTAAAAATAAATTTAATTTGAATACAATCATTTTTTCAGGAGATCAAGATTTAAATCAATTAATAGATGAACAAACAGCAATCATTAAAAAAGATAAAACTGGTTCTTTCTTTTTTCTTAATTCAAAGAATTTTTCAGAATATTATGATTTTTTACCAAATCAAGTTATTGATTATAAAGCATTAGTCGGTGATAACAGTGATAATTTTAAGGGTGTTGTAGGAATTGGTCCTAAATCTGCTAAAACTTTATTAGAAGATTATAAGACTATTGAAAATATATACAATAATATTGAAAAAATAAAACCTTCAATTGCCAAAAAATTAATGGAATATAAAGAAAATACATTTCTCGATAAATATTTAGCAACCTTAAGGATAGACTTTGATTTGCAACTTCCAGAATTAGAAAATTTAGCAATATCTAATATTGCAATTTCAAACAGTGCAAAACAAATTATTGACAAATTTGAACTATATTCTTTGGAAAATTCTTTGAAGAATTTAATCAATAAAAAATAACAAAAGTAGGGTTTTTCCTACTTTTGTTATTTTTGAAGTTCTTGCAATGTTTTTGCTACTCCAGATTTTTTATATGAATATCCTACATAATCTGCTTTTTGTTTTATGTTCTTTGTAGAATTTTTCATCGCTACAAAGCCACCTAAATATTTTTTAGTAACAACATCATTGCCCGAATCACCTAAGTGAACTGCTTCTTTATAATCAATATTTAACAAATCACATACAAATCGATCGCCAATTCCTTTATTAGCATTAATATCGCTAATTTCAATTGAATATCCTTTTGATACAATATGTGTTGAAATATCATGAAAAGCATTTAATAATTCATCTCTAAGTTTTATTATTCCTTTTTTTGACTTCCCAAAAGTTAAAATTTTTGTTGTTGCTGGCAATGATTGAATTTCATCATATGTAACTTTGTTTAGCTTCTTTAATCAAGCTCTAGCGATTCTAAATTTTGTTTTTGTTCCATATATTGTATCGCCACTATTAAAAATAAAAAACATTTTTTGTGCAATTAAAATTTCAATTATTGATTTCAATGTATCAGGGTTAATTTCATGGCGTTTCAAAACATTATTATTTTTATCTACAATTACTCCACCATTTTGGCAAACAGCAAACGGTGCATTTATTTTATTAGTTAAATCCAAAACAAACTTTGAATTTCCTCTACCTGTTGACAAAATAACAGGGATTCCTCTATTATTGAAGTCCTTAGCAACCTCAACATTTTTTTCGCTTATCATAATTTGTTTTTTGCGAGGCAAATCTAGATATGTGCCATCCATATCCAAAAAATATGCCTTAGGTTTAAAATCAATCATAATTTTCCTACTTATTTAAATTGTTTAAAATTTCTTCAACTGGCAAGTATCCTACACTTTGAGAAACTAACTTACCGTCTCTATAATAAAATAACGATGGAATATGAGATACATTCATTTCTCTTGCTAAATCAATGAATTTATCTACGTTCAAATCAAAAACATTAACATCTGAATTTTCTTCTGCTAATTTTTCTAATTCTGGGCCAATCATTTGGCATGGACCACATCAAACTGCTCTAAATGCAACAATTGATTTTCCTTCTAAATATTCATTCTTTTTGAATTCTTCAACTGATTCAATAGTTTTATACATAATATTTTCTCCTTTAATATGCTCTTGCAAATAATACAAATCTTTTTGTTTTTCTATTAGTTCAAAAACAAGTATCATTTGAAACTTTTATTGGTTCTTTAAATGGAATACACCTTGTTGATGCTCCTGTTTCTTCTTTAATTTTTACTTCATCATCACCGTTACCATCAAATGCAGCATACACTCAGTGACCCGCAGCAATTGCTTCCTTAAATTCTTGATAATTATTAACAGTTACAATATTGTTTTGTAAGCGTTGTTTAGCACTTTCAAATAAATTGTCTTGTATTTTTTGTAATAAGAGACTGGCAATATTTGCTGCTTCAGCAATTGTTGAAGTGATTTTTTCTAGGTTATCACGTCTAACGAATAATACACTATTATTTTCAATATCCTTTGGACCGATTTCTATTCTCAATGGTGTACCATGAATTTCTGAATTTGCTGCTTTAAAACCTGGACCCTTGTTTGAAGAATCTATGTTTGCAACAATATTTTTTGCTAATAAAGCATCTTTTAATTCAAGAGCAAGTTTGTGAACTCGTTCATCCTTTGAATTAAAAATTTCTAATATATCAACTTGAGTCCTTGCTATTTTGGGTGGAATGATTATTCCTCGATCATCACCATGAGTCATAATTAGCGCGCCAATTAATCTTGTTGTTGTCCCTCAACTGGTACCATAAGGATTGATTAATTTATTTTCATTGTTTTTAAATGTTATATCAAATGCCTTAGTAAAATTTTGTCCTAAATAGTGGCTAGTACCTGATTGAAGTGCCTTCCCGTCCTTCATCATTGATTCTAAGGTGTATGTTATATCAGCGCCAGCAAATTTTTCATGATCCGTTTTGCAGCCTTCTACTACTGGAATTGCTAAATTTTCTTCAATAACATCTTTATAAACCTTGTGCATAGTCTTGGCAAATTCAATGGCCTCTTCACTACTTGAATGGATAGTATGACCTTCTTGTCACAAAAATTCCCTTGTTCTTAAAAATGGATTTGTTGTTTTTTCTCATCTTAAAACATTAACTCATTGATTATAAATTATTGGCAAATCATTGTAAGACGAAACCTCATTTTTGAATAAATCAGCAAACAGCGTTTCTGAAGTTGGTCTTATAAAAAACTTTTCTGCCAATTTTTTGCCGCCTACTTCAGTAACAGTTGCAAGTTCAGGATTAAATCCTTCGACATGATTTTTTTCTTTTTGCAATAAGCTTTCTGGTATTAAAAGAGGTAAATAAACGTTTTGAACGCCTCTCTTTTTAAATTCAATATCCATTTTTGCTTGAATATTTTCTCAAATAGAATATGCCAATGGTTTAAAAATAATGCATCCTTTTGTTGCACCATATGCCATTAAATCCCCATTTTGGATAACGTCAGTATATCAACGCGCAAAATCTTCATTTTGTGGTGTTATTTTATCTAATTTTTTCATGTAATTAATTATAAATAAAAAAGATAAAATTAAAAGCACAATGTGCTTTAATTTATTTTTTAGATTAATTTGTCAAAAATATCTTCACCTGTTTGAACGGTTGGAACATTGAAATTTCTTGCAACAATATTTCCTGCAGTTCCCAAGCCTTTCAAAGGTTCTTTAAATACACATGGTGATTTGAATTTCTTTGAAAATATTGTCAATGGCAATGCTTCTCTTGTATGATCAGGGCCTTTTGTTGGGTCATTTCCATGGTCAGAAGTTATAATTAACAAGTCATCTGATTTCATTGCGTTTATCAATTTTGTTAATTTAATATCTAGCCTTGAAATATTTTGTGAATATCCTATTGGGTCTTCTCTATGTCCAAAATGGCTGTCAAATTGAACAAGATTTACAAATACAAATTGGTTTTTGGTATTTGAAGAAGCAATGTCAATTGCAACGTCCATATTTTCATCATCAGAAGCTGGTCCATAAACTTTGTCAATACCAACATGACTGAAAATATCATTAATTTTACCAACAGCAACTACTTCAACACCTGCTTTTTGTAAATCTTCTAGAATAATATGTCCTGTTGGTTTATTAGCAAAATCATGACGATTGAATGTACGTGTGAACTTTCCATTTTCATAAACAAATGGACGTGAAATAACTCTTGCTACATTTCATTCAGGTCTTGATGAACAAATTGCTCTTGCTCTCTTTGCATATTCAGTTAGTTTTTCAACACCCAATGTTTTTTCATCCCCAATAATTTGCAATGTAGAATCAGGAGAAGTGTAAATTATCATATCTCCTTTTTCAATATGTTGCAAACCTAATTCGGCCAATATAACTGTACCTGATGCATTTCTATTTCCAATAATTTTTCTATTATCAAACGCTTTTGACAATTCTGCAACTAAATCAGCAGGGAAACCTTTTTCAATAAATTGTGGGTTTGGAACTTTTGTATAAATTCCCATCATTTCTCAATGTCCTGTCAATGTATCTTTTCCATTTGACATTTCATAGATTTTTCCCATATATGCCAATGGTTTTTTATTTCTTGGCACATGTTTTGAAGTTTCTGCTATTTCAGTAATTCCTAATTTTGTTCATGTAGGAATCTCTAGTGGCAATGCTTCTGATGCATGCAACATGCTATTTGCACCTTCATCATGAAATTCAGCTTGGCGTGGTTCATATCCAATGCCAAGACCATCTGTAACTATAAAAAATATTCTATTAAATTTTTGTTCTTGCATAATTAGTACTCCTTTTTAGCAATCACAATAATAATTGGAATCTCATACTTATTTTTTAAATTGATTTTAATTTTAAATAAAAATAATTAATGTTTTAATAAGTTAGATAAGTAATTTTACTATTAATTTACTCACTTATTATTTTATTACAATAATCAATTTAAATTTATTAACTTTCAATGCATTAATAGACTTTATATTGTCTGGCGGGCATTCTATTGATTTTTTTAGCCTCCTGTGTGGTAAAATTAATATGTTAGATTTATGTATTTATTTAACAAAAGAGGTAATATGAAATTTAATGAACTAAGAACTAAAATTGTTGCAACAATTGGCCCTTCTTCAGATAATTATGACACAATGAAGAGTTTAATTGAAGCCGGAGTTACTACAATTAGAGCCAATTTTTCACATGGTGGTCCTGAAACTCATGTTAATAAATTTGAAATAGCAAGACAAATTTCAAAAGAACTAAACATTCCAATCTCATTAATGCTTGACACAAAAGGCCCTGAAATTAGAGTTGGAAAAATGGAAAATGATGGAGTTACAATCCCTGCAAATCACATTTTAAAAATTAAAACAGATGAAAATTCATACAAGAATTTTTTAGGTACCCAAGATCAAATTACTGTTTCATATAGAATGGACAACGATCTTAAAATAGGGGATAAAGTTTTAATTGATGATGGAAAATTAGTTACAACTGTAAAGGATGTTAAAAAATTTGAAATTATTGTTGAAACAGTTAATGAACATTTCTTAAAAACTAATAAAAGAATTAACATTCCTGGTGTTAACTTCTCACTTCCTTTTTTAAGTCAAAAGGATATTGACGATATTATATGGGGTGCAAAATACAAAGTTGATTATATTGCAGCATCATTTGTAAATTCTGCTAAAAATGTAAAAGAAATTAGAGACATATTAAACGCAAATGGTGGAGAAAATATTCAAATCATTTCAAAAATTGAATCTAAGATGGCAATTAATAACATTGATGAAATAATTGAAGCTTCAGACGGAATTATGGTTGCAAGAGGAGACCTTGGACTTGAAATTCCTTACTACGATGTTCCTTACTACGAAAAGAAAATAATTCGTAAATGTAGATTAGTTGGCAAGGAAGTAATAGTTGCAACTCAAATGCTAGATTCAATGGAAAACAACCCTATTCCAACAAGAGCGGAAGTTACTGACGTTTATTATGCAGTTGAATTAGGTGCAGACTCAACAATGTTATCTGGAGAATCTGCAAGTGGTAAATACCCTGTTGAAGCAGTTAAAGTTATGTCTAAAATTGCAAAAAGAGCAGAAAAAGAATATTACAATGAATTATTCTATGGCAAACAAATAGAAAAAGTGGCAAATCATCCTACTTCAAGAATGTTAATTGCTTATGAAGTTGCAAAAATCCTTCAAAGCGGTGAATACAAATTTGCTATTATATTAAGTCACACCGGAAAATTATTAAAAGAAGTTGCTAAATACAGACCAAACGCATTTATTGTTGGAGTTGTTAGCGACAATAGATTGGTTTGAAGTTTTGGAATAACTGCTGGTGTATTCTGTGAAAAACATTCAGAAGCAGTAAGAGCATTAATCAAAAACAATCACAATGCTGCTTATAAAATTTTAGGCGAATATGGCGCTAAAAAAGGCGACAAATATATCGTAGTGGATAGTTTAAATATCACAACCCATGAATACTAATAAAAATTATTAATTTCAAAACGAAAAATTAGGCTTTAACTAGGCCTAATTTTTTTGTCTTGGCTTAACCGGACTTACTTTTTTATATCGCTTATTTCTTATTTTTAAAATAAAAACATGCTTTTTATTTGTTTTGACTACCTTCTAAAAGTCGGTCGTAATGAGATAATTGCTTATTTTTTTGTGACCATATTTATAAATTTAAATTAATTAATTCAGAGAATTTTTTTATATAAATATTTTTCCCAACATTATTTTTTTCAAGCATTGATTTATATGTAGATAAAAATACGGATTCTAATTCTTTTTTTTGTTCTTCGTTAAATTTTAAAAAATTTACAAAATATAAATTAATTTCCTTTATATTTTGATTTGCGTTAACTAGGTTTTGTGCATGTTTTGATATTGAATAATTTTCACCATCAACAACTTCCTTCTCTGTTTTTAGTATAGTCGTTTCAACTATATACTGAATGTTGTTTTTTGAATCATAATAATCGAAATCTGCCGAATGTCCGGGAGCAGAAAAAATTGGATACAAATTCTTTGTTAATTTTGTATGACATATACTTTTAAAATTTTGCGGATTAATATTAAGGGAATATGTGAAGAATAAATTAACAAAATATTCTGCTAACGCAAAATTTGGCACGTGTTTTAGGGCAATGTATTTTTCTGATGGTGCTTCTTTAAATTCAAATTTTTCACTTTGAATTTGATTTAAAATATCTATTACTTCTTCATATTTAAATGGAAACTTGATATTTTTTTCAATATTATCCACCTTAAAAATTGGCAATGAATAAAAATTGGCTTCATTTAAATATTTCAAATTTTCTAAATTAAAAAAATTATTCAATTCAGATTTTAAAATCGTTTTTGAATTGCTATTATATAAACCCAATTTACACATTCATCTATTGAACATGTCCATATATTCTTTTTCGATATTAAATTCAATAGAGATTTTTTTTAAATCTAGCAAAAAATCTTCTTGTAAATGATTAAATATGTAAAAATTTATTTTATCTAAATAGTTTACATTTCTTTCTTTTGGGGGAATATTTAATAATCTTTTTATATATTTAAATTCTTTGGGTTTCAATTCTAAATAATCACTATATGACAAAGGTTCTTTGATTCTTAACAAATTAAAAATTTTTTCTAAAATCTTATATTCATTAATTTTCTTTTTAAATAAAGACTTAATATTAGTTATATTAGAAACATCTGCAATCTCTTCTAAAATTTGCTGTTTATTTTTTGTATATAGATCCTCAAATTTATCTTCTTTATAATCAAAAACCGCAAATGCTCAATTTAATTTTTCAATGTTAATCGATGATTCATTATAAGAAATTATAAAATTTACCATTTCTCTGAATTCTTCTCAATATAAGTACATCTTTTTGAAAAATAATTCCTCAACCGGGCTGTCTGTGTCAAAAAATTTTTTTGGTTCAAAATTAAAAACTAAATTTTTAGAATTTTTTAGAAATCCTCATTTTTTTAAATGACTTATCACGAAATTAAATCTACTTTTTATTTTTTTATCATTGCTATAATTATAGATTTTTTGATTTTTAACATATTTTTTAAACTCTTCGTCTCAATTTACATTGAAATTTTTTTGAAGATTGTTTGACAAATTATTTTCAATAGATATTCTCTCATAATTTACTCAAGATAAAATAGAAAAATATTCATTTATAGGTTCAGCAACTCTAAATTCAGTACCACCAAATTTATTAAATCCCTGCTTTTTCATAATTAGTTACATAAACTTCGTCATCTAGTTGATCTCTATTTTTTCTTTGATAATTTGAATTTGTGTATTGCTTATTTAAATGAAAAACTTTATATTTTTTTGACCATTCAATTAATTCAGTATTTGATTTACCCTTCGATTGAAATAAATTTGATAATCCTCATTTTATGTTTTTTGAATCTAACAAATCTAATAATTTAAATAATTTTCTGTCTTCTTCTAATGTTCAGGCCTGATTATATATTGCACCCGAAATTAAGTATGGAGGATCAAAATACCAAAAATCGTCTTCTCTTGTTTCTTGCATTTTCAAATATATAAATTCTTGAAAATCCAATGAAGAAAAATCAATATTTTTTCTATTTAAAGTTGAAGAAAAATCGACAAGGCATTGTTTCCTTGTTTCATTTAAAATTTGTTTTCCGGAAGGAATATTAAATTCTCCTTTTGAATTAAATCTTATTTGACTATTAAATCCATAAATTATTAGAACTAATAAAACTATACAAGATTCATATGAATACTTATTTTTTGATAAATTATATTCACTTTTTATCGAATTATAAATTGATGAATTTACTCTTTTCGTTACGGGATCTAATTTATCAATTGAGCCATACTTCTTATATATATATATATATATATATATCATTTAAAATTTTTATAGGGTTAGTATTTGAGAGAAGTTTTACAATATTGCACACTTCTTTTTGCTTATCATTGTAAAAATATTTTTGAGCTATAACATTTGCAATAACCGTTCCTCCTCCGGCAAATGCATCATAAAAATTTCTAATATTATTTGGAAATAATTTTTTTAATTGTGGTAATAATTTATATTTATCGCCCAAATAATACAATGGTGATCTATTCATTTTATTCATTTCTAGCTCATACCTTTCTATTCTTTGCTTTGCAGCACAAAAATATTTTTTATCTATCTCACATCCAATAAAATTCCTTTGCAATTTTAAGGCCGCAACACAAGTCGAACCAGAACCCATAAATGGGTCAAAAATTCAATCATTCTTATTTGTATGAATATTTATAATTTTTTCCATTAACTCTACCGGTTTTTGAGTCGGATGCAATTTATCAATTGATTTAGAAACGATTGAATTTTTAAATTCCGGTCTTAAATATTTTTTGCTTTTTTGTTTATTAAATGTTCATTTTTTGTTTCCTTTTACTGCTCAAATAGCAAATTCAAAATCTGTAACATATCTTCTTTCTACATTTCTAGGCATAGGGTTATTTTTTACTCATCTTAATAAATCTTTTATAAAAAATCCTTGATCTTCTAGTTCCTTGCTTATTTCGCCAAAATTTTTGTAATCATTAAATATTATTATTGAACCGCCATTTTTTATAAATGGGCTAATTTTCTTAATTCATTTAGTTTGGTCAAATTCATAATCTCATTTCCCAAAGTCTATTCCTGATCTGCCAATACTTTGAAAGTTATTTTTTTTAGATATATTATATGGTGGATCAGTTATAACAGCATCAAATAGTTCTATATTTAAATTTTCTTCTTTTAATTTCTTTATATATTCTAAGCAATCTTTATTCTTAATAATTCAAGTCATATCATCATCATAACAAAAAAAACGAAAATAACGACTATACAAAATTAAATTTTTTATAATTTTAGTTATAAAAATTTTCTATATAAAACTTTAAGTTCATATAGGAACAAATAGATATTCAACTCTAAATTTTCTTAATTCTCTTTTTTTATACACTTACTTTAATATTAGGTCGTGTTTATTATTAAAGGAATTTAACATGTTTTAAAAAAAGTAATGCTTCTTTACAAAAATATCTTATCATATTATCTAAAAAAAATAATAAAAAATTTCACTTTATTAGGCATCCGATTCTTAAAAAGGATTACTGTATAAAGCGAAAAAGGTGGGATTCCACCTGTTTTTTTGTTTATTAGTAATTAATCTGAATTTTGCTAATCAATAATATGAATTTCAATATTGTTTTTTATATAATCATAAGGATTGATTATTCTTTGAATAATAGTTTTTATATTACAAATAGAATAAAATTTCTTAGTTTTAGATTTTATTATATGTGAAACATAAGATTCGGCAATGTTTCCATAATAATTTTTGTCGCATTCTTCAATTTCTGATAAACCAGTAATGTTTAAATGTACATAATAAGCAAAATCGTGTAATAGTTTTGCTCTTTTTTCATTTAAACATTTTTTTATAGAATCTCAATGCTTAGACACTAGGTTAAAAATTTCGATATATTTGTGATTTTTTAAATATGTGTATAAAATATTAAACAAGTTAAATTTTGTGATTTTCTCTAATTTTTCAAGCTCTTTTTTTAAATGATTTGAATGATTAAAAATAACTTGAAATTTAGAATGTAAATGAAATTTACATAGAATATATCTTGCACTTAATTTTTTAGAAAGTGTTCGAATTCATTTTGCCCCGTCTCCTGTGATTATAATATTGGCTTCTTCTGAATAATTATTCCTAATTACAGAATTAATTTTTTCTACTAAATCATTTAAATTCATATTTCTATTTGGGCTCGAAAAATAAATCAAATGGTTTTTGCAATAAGGCTTTTTATTTTTATCTAATGCAAAGAAATTAATTATTCTTGCTTTAATTTTGTTTATTTCATTTTGACCTTGTCGTACTTTTAAATAAGTATCATCTACTGCAATATAAATATTCTCATTTTTGGTTATAATTTCCTTAAAATTAATTATTTTTGCTTCTTTTAAAGAATGAGGAAAATGATTTTTGACTATTTTAAAAATTTCTGTGTGGCTAATTCCATAATTTTTAGCTATTTTTCTATAACTCAGCCCGTTTAAATATAATGAGATTACTTTTTTACGTAATTCAAAGCCAATATTCTTATTTTTAATATCTTTAAACAAAGATAAACAAACATTATAATTTTCATATTTGTTATCTATTTTTCTTACATATTTTCTAATTTTGACTCTGATCGTTCCAAATTCAAATGTTAATTTTCTATAAATTCAATCTTTTACTCTTCAACCCTGATTTTTTCTAGCTACCGAGTTAAAGAACTCTATATCTTCATTAATTATTTTTTCTTTAATTATTTCTATCGCTTCCATATATTAATTTCATTTCAAATATAAAAAAACTACCTTATAGTAGTTAAAAAATCTTATCTATTTAATTTTTGCAGATTTTTTTCTATATATCAATATTATTAACGGTATTATTAAACCTATAAAACCAAGTAATGAAAAAGTTGTTATTAATCCTATTGATAATGCATTTAATTTTGGTTTTTGCTTTCAGTGTTTATCTTCTGTTTTAGATAAGTCGCCATCAAAATCGTTAATAACTTTTAATTTTGTTTCACCTTCGCAACTATCATTAGAAGGTTTTATTGTCAATGTTAGTTCATTTTTACCTTTTTTAGATATTAAGGCCTTCATGTAATCTATGTTGTTAAGTTCTTCAATTAAATAATCTTTTTCATATGCTAAATTGGTTATAAATAACTTAGATTTAATTTGACTATCTACTCATTGAATTATTAATTTTTTAATTTCTGTGTAATTTTTTGATTTAATGTTTAAATCATCTAATTCTAAATCACCAAAATTAAATTTTTGTGTTGTTTTATGGCCATTAGTAATTTCAATTGAAGTAGAATTTTTTATCATTGGATGGTTAGGAAATATATTCAATGTTGAAATAATGTCTTTATAAAATAAATTAGTAATTATGTTTTCAAATTCTTGTTCATTATAAATCACATTTTTAGTCAAAACTAATTCGGGATTTAAGGAATTTAATTGCTTTTGAATTTCTATTTTAACTAAATTTGCCATAGCACTTCATTTTGCTTGATATTCTTTATCATTTGAAAAAGAAACATCATAATTTATATCAATAGGGTCAAGTTTTAGTTTGCTTAAATCAATAATATTTGAAACATAAGTATCTGAAAAATTAATAATTTCAAATGTAGTTTTTGCTTTTATATTATGTTTATTATTATTTATATCAACATTTAGTTTTGTTGCATTGTTTAACGCAATTTCTTTTGTTTTATAAACATTTTTCAATTTTTGTACAATTTCACTTGTAATTTCTTGAATTTTTATATCATTTTCAGATACCTCAATATCAAATTTTTTAAAAAATTGAATAATTTGTTTCTTTATTTCAAGAGTAATTATTTGTTTTATTTGTTCTTCTCTTGTTTCACCTTTTAAATTAATCATTTTAGGTTCAAATCTATCATCAAATAAATCAACCGGTTTAAAAATTGCCTCAATTTTGAATTCGTTATTAGTCAAATAATATTTATCAGCATATTCTTTTTTTAATTCTGCATATATAGTTAATCATTTATTGTCAAAATATGCATAATTAAAATTTAATACTTGCTTTTCTGATTCTTTATTAGTTATAGCCGAAGTAAATGATGAAGAAAGTCATTTAAAAAATCTATATTCTGAATTATTCCTAATCTTTTCTTTGATTAATTCTTTATTTCATTGAAGAAATATTTCGGTTTTATTTGTTTTATCATTAATAAAATTAATTAAATAAGTTTGCTTTCTGGCTGTTAATCTATATCTATCATCAGCGGTTTTTAGATTGTAACTAATTTCCAGTTGTTTGTTGTCTATGAAATTATATTTTTCAATTCCTACTAATTCTTTATTTTCAAATTTTCCATATTTATCAATAATATCGATATTATTTTGTTTTAAATATTCTAAAAATTCAGTTTTATCCTTAAATTTATTAAATTCTGATGAAAAATCTAATCTAGGAGTAATATAAATGTTTTCATCTGGCTTTTCTCAGGTTTTTTTATCTTCTTCCGATAAATTGCCATCGAAATCGTTAATAACCTTTAATTTCGTTTCGCCTTCATAACTCATATTAACAGGTTTTATGGTTAATGTTAGTTCATTCTTACCTTTTTTAGATATTAAAGCCTTCATGTAATCTATGTTGTTAAGTTCTTCAATTAAATAATCTTTTTCATATGCTAAATTGGTTATAGACAATTTAGATCTAATTTGACTATCTACTCATTCAATTATTAATTTTTTAATTTCTGTGTAATTTTTTGATTTAATGTTTAAATCATCTAGTTCTAAATCACCAAAATTAAATTTTTGTGTTGTTTTGTGGCTATTAGTAATTTCAATTGAAGTAGAATTTTTTATCATTGGATGGTTAGAAAATATATTTAATGTTGAAATAATGTCTTTATAAAATAAATTAGTAATTATGTTTTCAAATTCTTGTTCATTATAAATCACATTTTTAGTCAAAACTAATTCAGGATTTAAGGAATTTAATTGCTTTTGAATTTCTATTTTAACTAAATTTGCCATAGCACTTCATTTTGCTTGATATTCTTTATCATTTGAAAAAGAAACATCATAATTTATATCAATAGGGTCAAGTTTTAGTTTGCTTAAATCAATAATATTCGAAACATAAGTATCTGAAAAATTAATAATTTCAAATGTGGTTTTTGCTTTTATATTATGTGTTTTATTGTTTATATCAACATTTAGTTTTGTTGCATTGTTTAACGCAATTTCTTTTGTTTTATAAACATTTTTCAATTTTTGTACAATTTCACTTGTAATTTCTTGAATTTTTATGTCATTTTCCGATACTTCAATGTCAAATTTCTTAAAAAATTGAATAATTTGTTTCTTTATTTCAAGAGTAATTATTTGTTTTATTTGTTCTTCTCTTGTTTCACCTTTTAAATTAATCATTTTAGGTTCAAATCTATCATCAAATAAATCAACTGGTTTAAAAATTGCCTCAATTTTGAATTCGTTATTAGTCAAATAATATTTATCAACATATTCTTTTTTTAATTCTGCATATATAGTTAATCATTTATTGTCAAAATATGCATAATTAAAATTTAATACTTGCTTTTCTGATTCTTTATTAGTTATAGCCGAAGTAAATGATGAAGAAAGCCATTTAAAAAATCTATATTCTGAATTATTCCTAATCTTTTCTTTGATTAATTCTTTATTTCATTGAAGAAATATTTCGGTTTTATTTGTTTTATCATTAATAAAATTAATTAAATAAGTTTGCTTTAAGACTGTTAATCTATATCTGTCATCAGCAGTTTTTAGATTATAAGTAATTTCTAGTTGATTGTTGTCTATGAAATTATATTTTTCAATTCCTACTAATTCTTTATTTTCAAATTTTCCATATTTATCAATAATATCGATATTATTTTGTTTTAAATATTCTAAAAATTCGGTTTTATCCTTAAATTTATTAGATTCTGATGAAAAATCCAATCTAGGAGTAATATAAATGTTTTCTTGATTTTTATTTGCTAAATACGTAATATATAAATTTCAATAAATTTGTACTTCTTCATATTTTAGCTTATCTAATTCATCCAATGATATGTTGTGTTTTTCGTTTAAAAACTTAGCGAAGAAATAACCAGTTGTAGTTTTTCATAAATTCTTAATTGAATCAATTTTGTTTATTTCTGAAAACATTTTCTTATTTATTGTTTGTTCATTGCTAATTTTTAAAATCTTGAAGTTATTAATTGAATCTTTTGAGCTTCTAGAATATAAGAGATATGTTCCTTCATTTGACAAATAGCTATCTCCTGAAACAAAATTATCTAATTTTTCAGGATTTCCTCAATTTTTGGATTTATTATCAATTTTAATTATGTAATACTCTTCTGTTTTTCCGGCAAGCATTTTTAGAGCAGCTTTTGAGATTACGGTAGCCTCTGCAATAAAGCCCTTATCTCGTATTTTATCACCCTTTGAATTAAACAATGTGTATGCAAAGAATGGCAATCCGGTCGTTTTTGCATATATATATTCAAAACTATTATTTGAAAGGTTTAGAGCGCCTAAATTATATGCTTGATAATTAGAACTTCAATCTTTTGTGTCAAATCAAACTAATTCTTTTTTTGTACCTGTCTTTTTGTCAATAAACGGGTCATATTTAGGATTAGGCAATGGATTACCATCCTTGTCCTTTTGTATATTTCCTTTTTCATCTTTTAAATATTCTTCAATTAATTCTCTTTGGTCTTTATTATATTTAGGGTCTCATGCGTATCATTTAAAATCTAAACTTCCACTTTTTGAATCAATAATATATTTTTTGGTATATGTTATAGTCTTTGTTGATGGATCGTTGATATTTCTATATTCTTCAACAGTAATTGTATATTCATTTTTTTTATGCGAATTCTTTTCGTCTTGTGGTTTGGAGGAATCAGAATCTTGATTGTATATTTTTTGGTCATCGCTTGCATCATCTCTTTTATCTGCAAGTTTAATATTGAATTTGTTATTAATTAAATCAACTTTATGGCCATTTACCTTAACAACATAATTGTCATTAGGCGCCGTAAAAGAAAAGACAGCAGGAGCATGTAATTTATAAGTTCCTAGGTAATAATTTTGTCCTTCAAGACCTATCTTTTTATCGCTTGCTATTCTTTCGGGCTTATCATTTGCCATTCCGGTAGGATTATTGTTATTTATTTGATCGAATGCTAAATATTTTTGAGTTTCTGATAACCTTAATTTATTTATTATTCCTAATTTTTCTAATTTATTAGATTTTTTAAAATCGAAACCCTGATTTTCAATCAATATAAATTTTTCATCATTTGATGTATTAACCATATAAATGTTGATAAATTTTTTGGATTCATCAAGCTTATAAGAAATTTTTCAAGTGAAATTTTCATAAGAAAATGATAAATCTAGTTTTTTTGCCCACTCTTTAAAATATTGCCTGTTAGTTTTAAATTGCTCATATTCAGGCTCTAATAATTTATAATCATCAATTTTGTCATGATTATAATCTGTAATTAATTGAACATTTTTTCCTCTTTCTATAAATTTAGAAAGTTCTTTTTTAAAGTATTCAAATGCTTTTTGGCGTGATTTAGGCCTCAATTTAATTTGTATTGTTATTTCTTTATTAATTTTTATAGAGTTAATGTATTGATAATAGAAGTCGCCTCTTCCTCGCCCTTTGTTAATAACAGATTGAGTTTTTATTCCAAATTCAACATTATTTTTTAAATTTATGTCATTAACATAATTTTTTCAAAAAGTATCAAAATTGATTGAAGATGAGGATCATTTATAGGGCAAATATCTATTATCTCAGTTGTAGTTATATGTTTTGTAATAACGTTGTAAATAATTGCTTATTTCTAATTCTATAACTTCTTGAACTTTTGCTTTGATATTTGAATTATTTAGCATGGTTCCTTTTATTTCATTATCTGTTGCTCATGGGGCCGGCATAGTAGTTTTCTTTATCCTGTTTCCTACTGTTCATCTAGTCGGGTCTGGTTTAGACCAATCTCCATTTGTGGTCGGGGTATGGCTAATATTGGTATTTACCGCAATGTTTAAAACTTTATCTTGGAAATCTGCACCGGGTATATTTTCAGTTCAATGATTGTAAAGAAAACCCGAAGCCCTTGACTGAATAAAATTGTTATTGGTCAAATTTTTATTCTTATTTTTTTTATCTTGACATGCTTTGATGCCTAGGCCTACTCCGGCAAGGGCAAGTATTGAAACTGTGCCTAATAAAATTGGTTTTCATTTTTTCATTATTTCTCCTTAATTATTTGCAATTGCTTTTAAATCGCCATTGTTAAATAATAATTTTCTTTCTAATTCATTGTAATAAGATTCGAATTGAAATCTTGTTGTGTTATCAATGGATAATATTCCGCCCCCAATCGGAGCAGAAGATAAAAATTGCAATTCTTGTTCTGTTAAAGGCATTCTATTTTCGAATAGAGTTTTTAATGCTTCTATATCTTGATAATTAGATCTTAGAGTAATTGAATATTGCATATTTTCTAGAATCCCCGCAGATTTAGTTTGAACTAATTGATTTTGATTAAAATCCATAAAATTTTGCGTTCCCAACACTAACATTCCTCGATATTTTCTTATCGTTTTAGCTGTATCTCAAACAAAATCTAATGCTATCGGATTTATTGGGTCAATAAATTTATGCGCTTCATCAATTACTAACATAATTCAATTTTTCCCTTCCGGATTTGAAAAGTAATTATTGGAAATTCTGTTTTGAATAAATGATAATATCAAGAATAATCCGGCCTTAGAAGCAGGTGAATCATCTTTTGTTGAAAGATTATAAGTATCTATAATATTGAATTTTGAAGAAAAATTAATATTTGTATAGCCATTATACAATGCTTCATAAGGAGAATTATTTGTAAATTCTGATTTTATTGTTGCTAAAACAAATTTTTGAGATTGAAGGTATATTTCTTTATCATTTGTATTATCAAACTCATATGTTTCTAGAAATGCTAAATAGTCATTAATTGTTGGATAATCAGTGTTTTGGAATTCTAAAAAATTGATATTTTCATCATAAAATCCTCATTTATTATATAACTCTGACAAGGATTTTCCGATAAATCTAACTTCTATATCATTAAAATTACACATTATTTTAAGGAATTTTTGAACTTTTCTAATATTTGAAGATATTATAATTTTATTTTGATTTTTAATTTCTACTTGTTCATTAAATTGCAAATCTAGTTGAAGAGGATTAATTAACGTATTAATTCCTGTTCCCATTTCAAAAACTTCGCCATCAAATAATTTTGCAAATTTTGTATATTCCCTTTGAGGGTCTAAAATAATGATTTCAGTGCCATAAATTAAATAATGCAATACCATTTTTTTCATAAGTGTTGATTTGCCGGCACCTGGTGTCCCGGTAATCAGCATATTTGAATAAACTCTTTGCGAATTTTTGGTTTTTATATCCAAAAATAAAGGTGAATCAGTTGTCATATCAAAGCCAAATAATAATGGTGTTTTATCATTTAATTCAGAATTGACAAATCCCCAACCTCTTGAAATATTTTCTGAAACCATTTCTAAATCATCCCCAATATTATCGCCATTTATCAAACAAGCACTTGCATATGCTTGTTTTTGTTTAAATAATAAAGGGTTTATGTTCGCTGATATTTCAGAAGCGTTTAATTGATTTTGGGTTTCTAATGCATTAATTTCATCCAAATTATTGCCCTGATTTACAAAAATAAAATTACTATTAAAAATATTCATATTTTTTGTATTTGCCAAATAAATAAGATTTTCAATTGCTTCCATTTCAACCTTTGATTTAACTGTTTTAAATCTTGATTTGCTATTGTTGTTTATTTCCACCATTGCTGATGCTTTATTTAATATCTTTTCTTTCAAATCATCATCAAATGCACTTAGATTTCAAATGACAGTTGAATTTGAAGAAAATAGTTTATAGGTTCAAGCATCTTGTAATTCAATGTTATTAAAATCATTTATTGATTGAAAACTAATATATTTATTATCAGCTACCATATACTTTTTAAAAAATTCTACATTTTTAGGAGCAAGTAATGGTGCTGGCTTTTCAATATTAGATAAATTTTTGTCATTTAATTGCACTCTAAATATTAAATTTAATACATTTAGTAAATCAATTCCTTTTACTAATTGAGGATTAAATTTTGTATTTGTTAATTGCAAAATTATTCTCGTTCTTTTAGCCATTAAATCTTCAATAGAATTTCCATAAATAACTAAATAATAATTATCAATGCTATCTTTTTCGTTTAATGTTTCTAATTCTTGTTTATTCAATAAACAATATTCTTTTGCTAGATTATTATTTTGAATAAAAGAGGTATTTAAATTTTTATCAATTTCATTAATATTAGAAGTTAAATCCATTGGCGCAGAGGTTTTAATAATAGTTATTTTATCATCAACAGAATTTAAGAGATTTGTAAATCTCAAAAACTCCGCTTCTCTATCTTGCTCCGAATTTACAAAAACATTAAATCCTTTTATCTTAACAACACTGAAATGATTAAATATTCCAAAATTATTCTTTCTATTAATTACAATTTCATCATTAATTATTTCGCTGTATGGTATTAAAAAACTAGTATTATTTTTTGACTTAATATCATATTTTCTTACTCCCACAAGATATTTAAAGGCTCTAAAACCCAGGACATAAAATCTACAATTATGTTTTTTTGAATATAGCAATATTCAAAATGTTGAAAGAATAAATGTTATGAATATTGCTATTTTAAATTTTCAATTGAATTTATTAGTAAAACCGCTTAAAACAATTGCTAATGCTATTATTGCAAAAAATATTACAAAATCAAAAACTGTATATTTTCTTCATATTGTTAATTTTTTATCTGCTAATTTTTTATTTTGAAGCATCTTTTTCATTTTCCTTTCTTACATCGTTTTTTTCTAAATCAAAATCATTTAATTGCTTTAATTGATTTTCCGCTTTTGAAATAGAGTATTTATATTTATTTTGAGTTTTGTTATGTTCCAATTCGGCTCGTTTTTGTTCTAAATAACTGTCTGTATTTGTTTTCTTGTAAAACTTATATGATTCATCATATAATTCTTGTTTGTCTTTGAATTTTTGTAATTCTGATGCCTTATATTGCTTCAAAGAGTCCAAATCATATAATGATTCTCTGTGTAGGTTTCCTTTTTCATCAGTGGCCATACTTTTAAAATTATTAAGCATTTTTCTTTGTTCTATTACTTTATTTTTGCCTACATTTTCAAACGAAGATTTAATAAATTCTTTGTTCATTCCCAAGTCTTTTGCATATGCTTTATTTCGTCTAATTCTCGATATATTAACCGCCTTACCGATAGAGCCTCCAATTGCGTTTGAAAAACCGCCCTTTGAAATAGTTGATCCTAATGTTGCATTGCCTGCTGCCACGGCTGCTCCGGCAGTTGTAGCGCCAGCAATAATTCCTGCGCCTGTTCTTATAAATCTACCTGCTTGTTTGAATCCTAAGTCAGGTACTTGAATATCTAGCAAATCGCCAATTAACTTATTAAGGGCTTTTGTAGCATATGCTGCCCCAATATAGATAACTAGGTCAGGTATTATTAAGATTCCTAAGTTAGTCAATTTTTCTTTAATTGGAAGCAAGATTAGAATAAATATGGCAAAGAAATTTAAAGCAAATAATGAAATAAAAATACTTATAACGCTTTCTACAAATTTGTGCTTTCAATTCATTATTGCTTTGCCATTATCTTCAACTGATTTTGCTGCCACTAATGGTGAAATCATAAATAAAGTAAATTGATTTAAACAATTTTTAACAATATTAACTAATAGGGAACCTAGCATAATTACACAAATTCAGGAAATTATTGTATTTTTAATAAACATTAACCCTAAGGGCCAAGAAGTTGGAACCAATCAGGCTACATATTCACTAAAAGGCATTGGAGCAAAATTATTCATCGCAACAGATTCTTTTGCTCATTCAGCATTATCATTTAATGCTACGTATAAAAGGGATATGATATCACCTTTCATATTGGTTTGAAGTTCGAGAGCTTGACCAAAATAGTAAAAAATTATTGCCAAAACTATATTGCTTACAAATATTAAAAAAGGCAATAATAGTGCTCATAGAAAAGCCGAAAATGATTGTTTTAAAACATTCTTGAATTTTAAATGTTCTACGTTTTGATTCTTGTTTTTCTTGCCAATTTGATATTTAGCAAATGAAATAATTATAAAAATAATAGTAAAAATTAATCCATATAGTAAAAACGCTAAAAACGCCGCTGGTAAATCTGAAAAACTAAATGTATATTCTCCTGGATTAACTTTGAAAATAATATAGTTAATTAATTCAAAACCAAAAAATCTTGCTACCTTGAACAAACCTTGCAATATAAGGAATGGAAAACTAATAAAAATTCCCCAAAATAATGCAAAAATTGCATATAAAATTGGGGATAATAATATGGTTATTGCTGAAATTAGCACTATTTATTCCTATTTAATAAAATAAGTATTACCAATACCAACAGTTATTAATACTAATGCAGCAATTATCAAGATTGCAAAAACTGATATAAATATCTTTGTTAATTTATCTATTTTTTTGTATTTTGCATCCGATTTATATTCTAATTTATTGTCATATTGAATTTTTATTTTTGTTTTCTGAGCAAAAAGAACAACAAGGTAAATAAACATTGCTAAAACTACTGGCGATAAGGCAATTGCTACTAAAAATGTTCAGTGAAACATATATCTCCTTTAAATTAATTTGCTAAAAATGTGGTGGCTACTTTTAAAATAACCGGAATTAAAACAACAAAGAAAATTAGGAAACCAAATGAAAGTCATATCCCCACTATTCTAGATTTAGCTTCTTTCTTAGAAGTTTGAGTTTTGGCTGTTTTCAATGAAGCAATCGCAAAGAATGTCATAGCAATAGCAATTAAGGCAAAGATAACAACAGCATAACCTGCTATCAGTGAAACAAATGGATTTATTTGCTCAAACAACGTTCCTAATGCTTGTTTAAGAGTTTCTACACCAGAGGTGCCACTATTCATAATTCTCCTTATAAAATTTAATTTTTGTAAAGATAGATAAAGTAAAAATACACTATTATTGATAGTGCTTACTTTTTTTGTGTTGTTCATACTCTTTACAAACTATAAGATTTCTTAATTTTTATTTTATAGAACTATATTTTAAACCTAATGATGTTTTATTACTATCTAGTCATTTGCTTAATGCATTAAATGCAGATTGAGCATTATAGTGTTTTAATGTTTTTATTTTAAAACCATTAGGATTTGAACCATATTCATATTTATTAAATAAATATTTGTCATATCAGTTGGCTTTATTTTTTAAAGCAGGATTAGGATTATTTCAAAAAAAGTCATTTGTTTCAGTAATAAAACCTAGTAAAAATGCTAAGGGATTATAAAATTCATTTAAAGCATCAATGATTTTATTGTAATGACCTGCCTTGTCATAAAAATTCTTGACAATAGGAGCAGGAAATACATAAAGTTTATTGTCTAATTCTAAGGCTATCTTTATATTGCCTATTAATTGTTCAATTGTTAAATTATTTTTTATCAGATGTTTTAATTTATCTAATTTCTCTGGACCCATTGCTTGTAGTTGCTCCAAGAAGGTTTTGTCAGGGTCATTTATATTGTTTATCATAAACGTAAATAAGTCAATTATTTTAAATAAAGAATCGCTAATTGTTCTTATTTCCATAAATGATTCAATATATTGAGAAAGTCCCAATGAAATAGTTGCTGTCTGAATGTTTTGCTCTCTAATTTCTTTTATTGTTTCACTTGAAAAGGATGATAATCAAGGATCTTTATCCTTAATTCTAGATTCGGTAAAATATTGATAATTTCCTTCGTAGTTTATTAAAAAATTCGGGTCATCTTCTAATACTTTTTTATTCTTAATTTTTCTAACATACTCTTCTTTTGACTTTTTAAACAAATCATCCCATCAATTACCTAGCAATTTATTGCGTGCTTCTTCTACTGCATTAGAATAATTTAAAATCACAGTTTTTGCTTGGGCATATCTTTGTAATTTTTGTTCTTTTGATTCATATTCATTTGTTTCTTTTCCATTAGAATCAAGGACTTTTAATTTATGAATATTAACCACATAATGTCAAAGTTGGTGAGGACCATCTCAATTTTTGTCAGAAACTGCTGGAACAGGACAAGAATATAAAAGTTTTGTATCGAATCTATCTTCTGGTTTTACCTCTTGAACCTTTGGCAGTTTTGGCTTTGGCAAATCATTTGGATATGAATTATTTGGTTTGGTAGATTTGGGCTTATCATACAATCAATCGCAACTAGCTGAAATTAACGGCATTGCTGTTATTGTAGGAATAATAGTTAATGTTAGTAATAATCTAGTTTTTTTCATAAACAATCCTTTATTTATTATATTTACTAATAAAATTATACTTTATAAACGAATACAAAAACTAAAACAAGATTATTGATTTTCTATATCTATATTGTTAATTTCCTTAACTATAATTGAAATTTCACATTTTCTTTTATAAAATATTGAATTAATTCTGCCTATCAAAGAAACCAGTTGATTTTCTTTTAAAAGCAATAAATTTGTATCATTTTCTTCAAATAAACAAGCCTTGATAGGCGCTGGTCATTTAGAAGAAGAGTTTTTTGATGGTTCAAAAAAGATAAATTCAAAATTTCTATATTTTAATCCTGTTTTTGATTCTTTTATTGCTGAGATATTAGAAATTCAACCTACCGCTTGGAGTTTATTAAATTCCATATTTAATTTCTTCCTTTATATTTTGATTCATTTTTTCAATTAAATCTAATTCATCTGTGATTTCTAAGAAATCTGCTTCTTCTAGAATTTTCTTTAAATTAAATATTCTTTGTTCGGTATTTTTGATTTCTTTTTTTAAATTTATTCAATTTTCCTCGGGTATTAGGTTTTTGCTTTTTGTAAAAAATTTCGCTCAGCTTTTTAGATTTTTCAAATTATGATTTCATTTTTTTAATCAAGAATTTACTCTATTTTTAGATTTATATAACATTTTAATCACCCTCTTTAAAATAATTAATTATTTTGCTTTTTAAATTCTTTTCATTCATTTTTTACTTGTGAAAATCAATTTTTTGTATATTCTTTAATAGTTTTCAATTCTAAATAAAAGAAATAATCAGTTCCGGGCTCGTTTAGATATTCGTTTATATCTCCTGCTTTTTGTGAAATTACACTGCATAAATTATTTAATTTTTCATTATTTTTATCGTTAGCAGCATTAATTTCCATAGCAAAATTGAATAGAAGTTCAGATAAATATTCTAATTCTTGCTTTTCAGCATTTATTGCACTTGGTAATAAAAGTTTAAGGTCCATTTTTTCTTTGCCGTGTATTTCAGGAGTACCAATTGAATATTCATAAGCTTTTTTAATGTCAAATTCTGGATTTTGCGGTTGAATTCATGGCTCTGGCTTCATTGAATAAAATGGTAATTCTAAAAAATGCAATTCGTATTTTTCAAGAATTTCAGGAATCCCCTTTGCGTATTCATAAATTTGTTTAGGATGATCGTTAATTAATTGAGTTGTCAGTTTTAAGGTTTCAGATAATATTGTGTTTGCTTTTGCATATTCTTTATAAGTGGCATTTTTAGAAGTCAATTTAATCGATTTTTCAATATTTTCATCAATTTTTTTTAGATGCTCTGATTCAACATTCAAATTTTGCAATTGGGATTTAGCATTTTTTAATAATTGCAAGTTATTTGCATATTCTTTTTTGCCATTTTCTAATGCTAATTTTTCATTGTTGCAAGATGCTGCCACTAATGGCAACGCAGTCGTTGAGACAGCAGCGATTAATGTAATTAATAGTTTTTTGAAATGTTTCATATATTTATCCTTTTCTTTATTATGTTTTAATAGTGCTTATTTATCTAATAAATTCCTCTATCATTGTCATAGGGATGTATAAAAAGCAGATTTGGTTTTTGTTCCTCTCGAATAGAATTAATAATTCTTTCGCACTCATTATTAATAGTTTCAATATATTTGTTTAAAAAGTGCTTTTTGTCTTCCTGAGAATAATCTAAATCATCTAATATATATTCAAGGTCATTACGAAATGCATCATAAAAACCGATATTAGGTTCTATTAAAAAACCTTCATGATAGCCCTCTTGATTGTTTCAAGATTTTTCAAATAATAGTTCAAATTCTTTTTGAGGAATAGAATAAGAATACCTATTAATGAGTGTTTTTAATAAATTATCGAAATCAATTTCTTTGGTTTTTACATCGTATCAATCATATATGTCGGTATCTATTTCCTTTCTATAATAATCGATAAATTTGTTTTCTAATAGTTTATTCTGTTGCAAATCATCAATAAAAGCATCATAAATGGTGTAAGTAATTGGTGTATTTTCAATTTTATCTATAAATCTTCTATATTGAATGTATGCATCTTGCTCATAATCAGTGTCTTTTATAGAATCTAATATATCAACAAAATATCCATTTACTCTATTTTCGCCATTTTCTCTTGAAACGAAATTAAAATACTGATCTTCAAAATTTTTTCTAGACATTTTCTCCACTTTCTACTAACTTGTTTTGTTTCAAATAGTACTTTGCATATTCCTTAATTAACGCCTCGTTAGAAAACAATAATCTTATATTGTATGCATCAATAATAAAAGGCAATATTGATTTGTTTGGTTTTACTATTTTCTCAGAGATAAATTTAGGGCTTCAATTAGCAAGTAATTCATTTAAGACCTTAAAATATTCAAAAAAGTCTTTGCAAAATTCCTTTGGCCAATCTTTTTCAAAATTAAATCATGATGATTTTCCGGCCAAAACATCACAAGCATCATATACACAATATTCAAAATCTGACAGTATTTTTTCTCGGTATATTTGAACAAATTCTTCAATCGCTTGTAAATGATTTTCTTTATATTCGGTGATTTTTTTTGAATTAAACTTTTTTTCAGGCAAAGAATTAAAAAAATTTCTTAAACTTTTGCTAACTATTGACATATTTTTATTCATTATAATCTTTATCTCTTTCAGAGTTATCTGTATATTTTTGTGCATCTTCTCTGGAAATTATGAGTGCTCTATTATAAGCATTAAATGCAATAAATTCAACATTAAAGGTTTCTATATTTGAAATTTTTTCAATATCGAATGCCCACCCCTCCGCTTCCATTCCTTTTTCTTTAAGTTCTTGATTTGCCCATTCGTATGCAGAATCAAAATCTAACACGCATTCAGGACTATGAGTTTTTCAATAATCTAAATCTTTTTTTATTTCTGACAAAGTCATTATAGCCATTTATCTCCTTATAAAGTTTTTGATTTTGATTGGGATTTAGAGGATTTGTTTTCCTCTATTTCTTGATTTTGAAAAAATCTTTCGGCATCTTCTCTGCTCATGATGAAAGCTCTACCATAGGCGTCAATGCCCACAAATTCAATATTATAAAAATCGTTTTGCATTTCATAAATGTTATTAATGTCAAACGCTCATAAATCTGCTTCCATTCCGTATTTTATTAATTCTTCGTCTGCTAATTCTCAGGCATCGTTTAATGTATAAACGTCTTGCGGTTCGTGTTCTTTTCAATAATCTAAATCTTTTTTTATTTCTGACAAAATCATTATGGCCATTTACCTCCTTTATTTTTTAACAATTGCTTTTTACTACAAAATAGATTTGGGCTTATATTTTCCGTAATCTTTCTGGTGCTAAAATTAACAATTAATAAAACAGAATTTTTAAAGTTCAAAAACCACTACTTTTTTTAAAATATGCTTATGAAAGGAAAGAAACGAAAACAATTATTTATAAAATTTTTTTATTATTAATGGATTTAAAATTTAAATTTTCTTTTAATCAGATTTATAAATAGCAATTAAACTTTTGCTTTGATTAAAAAATAAGATTTAAATTTCTTCTCCGAATTTAATTTAACAGAATCTATTAATTAATTGTTCTATTTCAAAAAGACAATTCTCTGATTGAAATTTTTTAGCACCAGAAAGATTACGGAAAGAATTTTTAGTAATTTTTAAAAATTTGCTTCGAATTTAAGATTTTCGACTTTTTAAATAAAATTATTTAATTTTTGCTTCGAATTTAAGATTTTCGACTTTTTAAAAAATATATTAAGAAATTTTTCTCCGAACGAAATATTTAATTATCCCTGCTGAAAAAAATATTTGTAATATAAATTATTCTTTTCTTAAAATCTTTCAACCTAGGGCTTTTAAACACTCTTAAAAAGAGTTTCAAATTAAATCTTTGCTTCGCTTTAAGACTTTTAGAAATCCCTTATAAAATTTTTCTATCTTTGAATAAAGTGTCCCTTTTAAAAAAGGGAACAAAATTCTTAGGGTTCAAGCCCCCTTAAAGGTTGCTTTCACCCTTTTGAATTAAGGGTTATTCATATTCTATTCATAACCCTTAATTATCTTAGGGACATATTTTTGACAGTTAATTTTAAAAAAGAGTCAATATTAATAATTCTTATTGAAATTTAGTAATTTTTGCCAAAAATTTCAATATCAAAAAGATCTATTTTCAAAAATAGATAATAAAACCTAAATTATTAAAACTTAATTATAATTAAACGATTTGTTAAACAAACCTCTATTCTATTACATAAACCCTAAAATGCATAATTATTATTAAGTCTTATCAATAACGTTCTTTCTGTTGTAAATTTTTGTGAAACTTTGTCGAATTTATGTTCAACTTTAAACAAAGTTTCATATTTTTCTTGGCCTAATATTTTTAATAAAGAATTAATAAATTTATAAGCACAAGATTTTTTCATTTTTAAAAAGTTATTTTGAATTTTAATTGATGATGATTTATTTGTGGTAAAGCTCTTTTTTGCTACTTCCTTACTTTTTCATACAATTAAATTTATTTTTTCTAAATATTCTAATGGAAAAAGAAGAATAGATCTTCAAACTAAGATTCCATGTAGTTTAAAATATGACTCACCTTTTTTAAATCAGTACTTTTTTGAAATTCTTATTTGATTATCTTTTTCTAAAATTAAATTTAATTTATTTAATTGATTAATGGCTAAGATGATTGAAGTTCTACTAAATCCAAAATCAACTAAAAATTTTTTACTAATTCATTTCTTTCTTGAATATTTAATAATAACTAGATAAACTAAAAAAGTTAATTTTGATTTTTTTAATAATTCAAAAATTTTATTACTTATTAATATATTTCATTTAAATATTTCATTAATTTTATCTTCATTTAGTTTTAAATAAATTTTATTTTTATATATATTTCAAAAATTTGAATCTCCTTTATATTCAATTGAATCAAGTCATTTGTCTTTTTCAGTATAAATTTCTCCTGTTAATTGACTTCATATGGCTCGATTTTCTTTATTCCTACTCACAATAAACTCTCCTACTTAAAAAAATTTTTAAAGATTTTTTAATAAAGAATTTATTATGGTATAATTTAAATGAGGTTTCAAGGCATGTGCCTTGTTTTTTCTTTATTAAAGAAATCTTATAGTTTGTAAAGAGCATGAGCAAGAAAAAAAGTAAGCTCATTTTACTTACTTTTTTAAATTTTGTTCATTCCTTATATTTTTTACACGGCCTAATATTATAAAAAACACCAAGTGTTAAAATAATATACTTGGTGTTTCGCTTTAAATATTCTATTATTTAATAACTTCTTTGCCCATGTATGGAACTAAAACTTTTGGAATTGTGATTGAACCATCTGGGTTTTGATAATTTTCTAAAATAGCAGCAACAACTCTATCAATAGCTAAGCCTGAACCATTCATTGTGTATGCATATTTTGTGTGACCATTTTCATCACGATATCTAATTTTTGCCCTACGACCTTGGAAATCTCTGCATATAGAAATTGAGCTTGTTTCTCTATATCTTTGTTCTGATGGAATTCATAGTTCTAGGTCAATAGTTTTTGCTGAGCTAAATCCAATATCGCCAGTTGAAAGCATCATTTCACGGTATGGAATTTCAAGCAATTCAAGAATACTTTTGGCATCATTTACCATTGATTCTCATTCTTTCATTGCATCTTTTTCACTGGTTATTTTTACAAGTTCGACTTTATGGAATTCGTGTTGTCTAATTAAACCTTTTGTATCTTTTCCACTGCTTCCTGCTTCTGAACGGAAACATTTTGTATATGCAACAAATTTCTTTGGCTTAGACAAATCTAAAATTTCGTCATAATAATAATTTGTAACTGGAACTTCTGCAGTTGGTATTAATCAAAGGTCATTGCCTTGCATTTTAAATAAATCTTCTGCAAATTTAGGCAATTGCCCGGTTCCATAAAGCATATTTGAATTAACTAAATGGGCAGGCATTAATTCTTTATATCCATTTGCAATGTGCGTATCAAGCATAAAAGAGGCTAAGGATCTTACTAATTTAGCACCATCATTTTTGTATAAAACAAATCTTGTGCTTGCTAACTTCACTGATCTAACAAAATCAACAATATCTAATTTTGTTGCTATTTCATAATGTGGCAATACGCCTTTTACTAACCCTCTCCCTAAATTTGGATGTTCTTTTAAAACTACGTTGTCATCTTCATCTCTTCCCACAGGAACTTCATCATATGGCAAGTTAGGAATTTGGTATAGTAATGTATTGACTTTCTTTACCAATGTGTCAGCTTCTTCATCAATTTTTTCAATTTGTTCTTTAACTTCAGCAACTTGTTGTAATAATTCCGAGCAATCTTGCTTGTTTCTTTTATTAATTCCAATTTGTTCACTTAATTTATTTCTTTTAGCTTGCAAAGTTTCTAAATTATGAATTAATTCGTTTCTTTTTTGAGCGTCTTCAAAAATTTCTTCGATAATAGCAATATTGAAATTACGTGTTGATAGTTTTTTAATAACTTCATTTTTGTTACTAATAATATATTTTAAATCTAACATTTTTAATCCTCTTTTACTTTTTAAATGCTATTATATTTTACAATATTATTAAATAAATGAAATACTTAATTTTTGTTAAATATATTTAACTTTTGCTTATTTTATATATAAAACAATAATTAATTATTATTGATAACCAAATTCTTATATTTGTTAAAATTTTAAATATGTTACAAAATAGCAATATTCAAGGTAAATTTAATAAAATAATGTGAACGTCCACTGATGGCCGAGTTGTTATAGTTTCATTTTTAGTATCTTCTAATGATGGTTTGAATCCTGTTAAAGTTAATAAATACAACAGTATTTCAGTAACTTTAAAAAACAATTTATTTTCTGAAAAAAACATTACCTTAAACAGCCACATTTATGAAATATCAGTCTCTAAAAATAGTTTTTCAAAATATCCAAATAGTTATATTGCAACAGACGTTATTGAAATAAACGAAGATGAAGAATATAAACTAAACTATGTTATTAAATTTTTAAAAAGTAGTCATTTTCCTGGTATAGGCGAAGAAAAAGCAAAATTAATTGTAAAAGATTTAGGGATCGATGCTTTAAGAAAAATGGTTGAGGATAATTCAATTGATTATTCAAAATATTCAATATCAGAATTGACATGAAACTATGCTGTTGAATTTATTAAAAATAATCCAACATTAATTGAGGATCAAATGTTCTTTTTAACAAATAATTTAACGCCTTCTTTGTATGAAATAGTTGCTAAGAAATTTGAACATTTTAAAGATTTTATAGATGCTTATAAAGATAACTTTTACTTATTTTATTTAGACAATGAACGTATCTCACTTGAAGATATGGACAAATTACATAATATCTTTAACAAATTTGAACACCCCTTTAAACCTGCAACCCACTTGTACAAATCATTTTTAAAATATCTATATAATTCTGGAAACACTAGAATTTTAAAAGATTATTTATACGACCAACTAATAAACTTTGCAAGCCAAGACACTGAATGACCTAGGGATGTTGAAACATTCAATAATAGCGTTGAATTATTGGTTCAAAATAAATATTTAATCGAAATTGAATACGAAGATGGTACATATCTTAGTGCTAAAGATGTATTTGATATGGAAAAATATATTGTCAAAAGATTAAAACATATTGAATCTTCTTCAAACCCAAAATTCATTAATTTCAAGCCAAGCGAAATGTTTCACCCTTTGCAACTTCAAGCAATTGAAACAGCACTAACTAGCAAATTGACATTGATTACAGGAAACCCCGGCACTGGAAAAACATTGATAACTAATGAAATAATTAAACAATTGCTTTCAGTCTATGATGAAGATGATTTGGCAATAGTCACACCAACAGGTAGAGCAACTATCAACATAAACAATATTCAAGAAAAAACAAATGCAGTTACTATACATAGTTTTTTAGAATGGGATGTTGATAATGACAAATTCATGATTAATGAAAAAAATGCAAAACCGATTGAATGTTTAATAATTGATGAATTTTCAATGGTAAGTGTTGATTTGTTTTATTCACTACTTAAAGGAATAAAAAAGAATTCATTAAAACGAATCATATTAGTTGGTGACAAAGATCAATTGCCTGCCATTGGCGCTGGTTATTTAATTAGGGATTTTATAGAAAGCAATATTTTTCAAACAATTGTTTTAAGTAAAATTTTTAGACAAGCAGAAAATTTTGACATTATTCAAGATGCTCTAGATATTAATAAAGGCAAACTACCCGAATTTAAAGGAAATAATTCTCAGTTTATTGAAACGAAACAGAGTGACCTAAAGTCAATGTTACTCTTTAAATTAAATGACTTATTGCAACAAGGCTATAGCAAGCGTGATATTGCAATTTTAAGTCCAATATATAATTACGAAACGGGAATTGATCAAATAAATGAAACATTAAATAATTTCTTTAGAAAATTAGATGGCAATGAACAAATAAAGTATCGAGACCATGTTTTGGCCATTGATGATAAGGTAATAAATTTAGTTAATGATCCAAAAACAAAAGTGTTTAATGGAGAAATCGGTTATATATCTCACTTTGGCTTTGAAAACAAAAAAAATTCTTTTGAAAAAGTTTTGGCAAGTATCTCTGTTGAATTTGAAAATGATTCAAAAACTGTCTCTTATTCTAGAAAAGATTTTTTTGCTAATACATACCCTGCATATTGCACAAGCGTTCATAAATATCAAGGTAGTGAATGTAAATGCGTTATTGTAGTATTATTTTCAGAGGCTAAAAAATTATTATCCAAAAAACTTATTTATACTGCTATTACTAGAGCAAAAAAATATTGCGTTGTTATTGGAGAAAAAGAAGCTTTGATAACCGGTATAAATAATGATGATGATTCAAATAGAATAACCAATATTAAATATTTATGGAGGAATGAATAATGAAATTAATTGTTGGACTTGGAAATCCTGGTGTTGAATATGAAAAAACACGCCACAACACAGGGTTTATGGTAATTGACAAACTAGCAGAAAAATTGCATACTCCCTTAACTGAAAAAAAGTTTAATGGAATTTATTACATAGATAAAGAAGTAATTTTAGCCAAACCATTAACCTTTATGAATAATAGCGGTGATTTTGTTAGAGCAATAAAGGATTATTACAATATTGTAGATGATGACATTATCATTGTATATGATGATTTAGATTTGCCAGTTGGAAAAGCAGTTATTAGACAAAAAGGTTCGTCTGGCGGACATAACGGAATGAAAGATATAATTGCTAAATTAAAAACCGATGAAATAAAAAGATTAAAAATAGGAATTGGACGTGGTCAAAATGTAATTAATTATGTTCTAGGCAAATTCAGCTATCAAGATTTTTGCACAATTGAAAAAATAATTGATATTGCTTCTGATGCATTAATTTCTTTTATTAGTAATGATATTAGATTTGTTATGAATAAATTTAGTGGTGAACTATATCGTGAAAACAAATAATTTAGTTGAATTACAACATGACTTATTAAAAGAATTTAATAAATACAATATTGATGTCAATAAAAAATTTCTAATTGGGGTAAGTGGTGGTCCTGATTCAATGTGACTACTTAATTTGCTTAAATCATTGAATATTGTTGTTGCACATGTAAACTACAACAAAAGATATGATAGCAATTATGATGAATCATTAGTTTCACGATTTTGTGCTGATAACAATATCCCTTTAGAAATACTTTCATTACATAACAAATATTCTAGTGGCAACTTCCAAGCTATTGCTAGAAAAGAAAGATATGATTTTTACTTTGAAGTTTCTAAAAAATATCAATGCGACTATATCCTCCTTGCCCACCAAAAAGACGATTTTTTAGAATCATCTATTATGCAAGAAAATTCAAACAGAAAACCATTATTTTGGGGTATTAAGCAACTTAGCATACTAAATAATAACGTCATTTTTAGACCAATGTTAAATTTATGAACTAAACAGGAAATTATAGACTTTTGCAATGAATATTCAATATTATTTGCAACTGATTATACCAATGCCGAAACTCATTACACTAGAAATAAAATTAGATCGAACTTAAAGAAATTAGATCCAGAGCAAAAACAAAATAAATATCAATTTTTTGTCAATAAAAATATTGAAAATGAACATATTGAAAAACAAGTTTTAGAAAGTTTTGAAGATTGAAAAAATAATCAATTCAATGTTGATAAATTAGATAAGAATTTTGTTTATATTGACCAGTTATTGTTTTATACATTAAATCATTTTTATAACAATTTAAAACTTTCATTAAATAAGATACACGGAATTAAAGATTTTTTATTTTCACAAAATAGAACAAAAAATTTTAAATTAAACGATGAACAATATATTCATAAAATTAAAAATTTACTAATATTTAAGTAATTTTATTATAATTTACATATTAAATTATAAACAAATAGAGAGGACAAATGAACAATCCAAAAGAAAAAAAACCTAGGAAAATTGGTTTGTTTGGAATAATTGGAATTTTATTTGCAATTATTGCAGTTATAACATTGGGATATATTATCAGTGATGTTGTTAAAGAAAGAATGTCTAACTTTCAAACCTTAAATTTTCTAGAAAATGTTATTAGAGGAACTGCTAACAAAAATGATGGAAACTATATTTCTTCATGAGTTGAAGACCCTTATAACGATACAATAAATATCACTATTAGAGGTACAAATAGTTTTTTAAGATCCTTAATTGGGAATAATTATTTTGTTGGCGATTCTTATAGTTTTATAGTAAAAAATGGAGGCTATATAGGTCGTTCAATATTTCAAGAATTGCCTGTATGAGCAGGTTTAAGTGGAAAGTCATATATAGAAGCATTGAATTCTTGTGCAACATTTAATGGTCAAGTTTTAGGAAATATTGTTGCAAGTTCAATGCCAAAACCAAGCATTGCAAGCAAATATATAATTCCTGCTTTCTCTGCTTTTGTTGGCTTAGCATTTATTATTATCATTATGGCAATGTATTTTGGCAAGGGAAGAGGAATGCTAACTGGCGGTATTGATACAAACAAATCATTAGCGCAAAGAATATATTCAAATAAAAAATTCTCTGACATTGCCGGAAATGAAGAAGTAAAAGAAGAAGTAAAAGAATTAGTTGATTATCTTAAAAATCCTAAAAAATATGAAGCAGCTGGCGCTAGAATTCCAAAAGGAATATTACTAGGAGGCCCCCCAGGAACTGGTAAAACTTTAATTGCCAAAGCAACAGCGGGAGAAGCAAACGTTCCATTCTTCTTCATCTCGGCATCAAATTTTGTTGAAATGTTTGTTGGATTAGGTGCAAAAAGAGTTCGTGACATGTTTGCTGAAGCTCGCTCTCATGCTCCTGCAATAATTTTCATTGATGAATTAGATGCTGTTGGTAGATCTCGTGGAGCTGGAATTGGTGGAGGTAATGACGAAAGAGAACAAACTCTTAACCAATTACTAGTAGAGATGGATGGTATCAAAGAAAATAGTGGTATTTTAATAATGGCAGCTACAAATAGAACCGATGTTCTAGACCCTGCTTTATTAAGACCAGGACGTTTTGATAGAACTATTACCGTTGGCCTTCCTGATATAAAAGAAAGAGAAGCAATTTTAAAATTACATGCTAATGGAAAAAGAATTGCAAGCAATATTAATTTTGCTATGGTAGCAAGAAGAACACCTGGTTTCTCTGGAGCACAGCTTGAAAATGTAATTAACGAAGCCTCTTTATTAAGTGTTAGAGAAAATACCGGAGTTATAACTATTGAACAAATAGATGAAGCTATTGACAGAGTTATTGCCGGCCCTGCTAAAAAATCAAGAACCATTTCTGAAAAAGAAAACGTTGCAGTTGCCTACCATGAAGCGGGACATGCAGTTGTTGGAATAAAAATTAAAGGTGGAAATAAAGTTCAAAAAATAACTATCATTCCAAGAGGAAAAGCTGGCGGATATAATTTAATGATGCCAGATGAAGAAAAATACAATCGCTCGAAATCAGAATTACTAGCTATAATAACTTCATTCATGGGAGGACGTGTAGCTGAAGCAATTATTTATGGAGCAGATAATGTTTCAACTGGAGCAAGTGACGATATTGCTAAGGCAACAAATATTGCTAGAAAAATGGTTACTGAATGAGGTTTATCAGATCTAGGACCAATTAAATATGAAGAAGATTCTTCAAATCCTTTCTTAGGAAGAGACTACATGAAAAATTCTTCATTTTCAAGTAAAGTAGGCCAAGAAATTGATGAACAAATAAGAAAAATTATTTTAACTGCTGAAAAGAAAGCACATGAAATAATTACAGAAAATCGTCAATTGTTAGAATTAATTAAAGACGCATTAATTATTAAAGAAACTATTGTCGCTGAAGAAATTGAATATATAGAAAAACATATGGAATTACCTCCTGAAATAACAAATACAAAAAAAGAACTTAAAAATGAATATTCAGAAGAAGATTTCAATGAATTATTTAATGAAGTTGCTGGACAAAAACAAATTCAAGAAGATAAATACAAAGCTACTTTAAACTCTGAATTAAAGAAACATAATATTGAAGATAATGGTTCAAGCAACCATTCAGAAGATTCTAATGATACAAAAAATTCAGATGAAGACAGCAACAAAGAAGACAATCAATAAAATAAAAAAGTGGAATTCCACTTTTTTATATATCTCCGCTTTCTCGCAACATCTTTTCAAGGCCGTTAACAAGTTTCTTAGAAAAAACTCCTGTTCCTTTTTGCTTTTGTTCATATAAAAAATCTAATTCTTCATACAATTTATAAATTAATTTCAATGAATAATTGTATTGACTAATTGAATTTTTAAAATCTTGCCAATCTATAACGCTAAAATCTTTATCATCATAACTTTTAATATCGATATCAAAATCAATATATTTAATTACTTCATTTTCAATATAAAAAGGGCTTGCTAAATTAATATAAATATAGTTTTCTTTAGATTTATTTAATGTAATAGAAGCATTATAAAATTTGTTTCTAGCAAAGAAAAACAAAATAGGTTCAGAAACAACTCAATTGATAGATTTTTCAGAAACTTTTGTTTTCATTAACAAAACAACTACAAAGTCAGTTAAATTTGCAACTATTTTTGCCCCTTCATATTGTCTGTATAAAGTTCCATCATATTTAAAAACTTGAATGGTTGTTAGTTGTCCCATCTTAAAAAACTTTTTATGTTTTTTTATATTTTTTGAGTTTGGTGTATCAATTGTTTTTAATAAATCATCAAAACTCTTTATACTTTCTTCTTCCATAATACTATTTAAATGGTATCAAATAAAAAACAAAAAGATTAATTAATTAAATTTTATTTTTAATATATTACAAATTTAACAACTTGCGATTTGCTGTATATGTTATAATAATTACGTGATTTAAACAAATTAAGCATTTTAGAATAAATCTATGTATGCTTTGAAAAGTAACCTTTGGTTGCTTTTTTTGTTTATTTAGTGTATTATAATTAATATGCGACTAAGATTTAATAAAAATGCAGAAACATCCTTAATGGCAAGCCCAATGACATTTAAGGATTTTCCTATTGATAATAAAAAAAATACTATATTAGAGATTGGTATGGGGAGAGGAACCATGCTTACAAAATTAGCCCTAAAGCATCCTGACATAGAATATATTGGACTTGAAAAATACAGCACGCCAGCTTATTCTGCTTTAAAAAAGGCTATTGACCTCAACCTTGAAAATTTTCACATAATTATAGGAGACGCAATAAATTTATCAACTTATTTTAAAAACAAAATTAAAACTATTTGATTAACATTTTCAGATCCCTGACCTAAAAAAAGACATTATAAAAGAAGGTTAGTTTATAGAGATTTTTTAAAAATTTATCAAAATATTTTAGATAAAGATGGGGTAGTTTATTTTAAAACCGATAATGATATGCTTTATCAATTTGCAATAGATGAATTAAAAGAAATTAATGCAAAAATCATTTATCAAACGTCTGATTTACATCATTGCAATTTTGAAATAGAAAATGTTTTTACTGATTATGAAGAAAAATTTAACAAATTAAACAAAAATATAAACTTTATTGCATTTACTTTCAATTAAAAAGGCCTGTAACAAAGGCCTTTTTTGTTATAATTATATAATAATATAATTATATTGTGTAAATGGAGTAAATTATGCTTTTTGGCGAAGATGATAACAATAAAAAAGAAAATAAAAATTTTGAAGAAGATAACAGCCACGATTTAGATGAATATACAAATTTTGATGATAGAATCAAAAAAATCTTTAGTGACGATGATGAAACAAAAGCAAAAAAAATAGATGAAGAAGACGAAGAAGAAATTCCTCAAGACAAAGAAGGCTATAAAGTAGAACCCCAACTTTTAGATAAAGAAATTAATGGTTTAAAGCCTGCCAATTTATCAAAAGTAATGAAAACATCATTCATTGAATATGCAATGAGTGTCATAGTTTCTCGTGCTCTCCCTGATGCTCGTGATGGACTAAAGCCGGTGCACCGTAGAATTTTATATGGCATGAGCGAACTTGGAATGTTTTATACAGCGCCACATAAAAAATCGGCAAGAATCGTCGGAGATGTTTTAGGTAAATATCACCCACATGGAGATTCATCAGTATATGAAGCTATGGTAAGAATGGCTCAAGATTTTTCACTTCGCTATCCTTTAATTGATGGACATGGTAACTTTGGATCTGTTGACGGAGACGAAGCTGCTGCAATGCGTTATACAGAAGCTAGAATGAGCAAGATTGCTGGTGCAATGGTTGATGGTATTAAGAAAAATACTGTTGACTTTATGGACAACTATGACGCAACTGAAAAAGAACCAGTTGTTTTGCCATCACGTTTCCCTAATTTATTAGTTTCTGGTTCAAGTGGTATTGCTGTTGGTATGGCAACAAATATTCCTCCTCATAACCTGGGCGAAACTATTGATGCAGTTTGTGCACTTGCAAAAAATCCAGAAATAAATGTTTTTGAATTAATGGAATATATTCAAGCTCCTGATTTTCCAACAGGTGGAATAATATTTAATAAAAAAGGATTAATTGAAGCTTATAGCACAGGTAGAGGATCAATCACCATTCGTTCAAAAGCACACATACAAGAACTTTCAAACGGAAAAAGTAAAATAATTGTTACTGAAATTCCATACGAAGTTAAAAAAACAGAAATCATGGAAAAAATTTCTGAACTTATTAAAGATAAAAAAATTGATGGAATTGCTGATTTTAGAGATGAATCGAACAGAGATGGCATTAGAATTGTAATTGATATCAAAAAATCTTTTGTTCCAGAAATTGTTTTAAATAACTTGTTCAAATTAACAAAATTACAAAGCAATTATTCATTCAATATGATTGCGTTGGTAAATAATGAACCAAAATTATTGAACCTTAAGGCTTGCTTAGAAGTTTATTTAAAACACCAAATTGAAGTTGTAAGTAGAAGATTAAAATTTGATCTTGAAAAAGATAGAGCAAGAGCTCATATTTTAGAAGGTTTAAAAATCTGCATTGAAAATATTGATGCCGTAATTAAAATAATTAAAGAATCGAAAACTGACCAAGAAGCACAAAGCAAACTTTCTCAAGCATTTAATCTTAGTGAAATACAAACAAAAGCAATTGTTGATATGAAGCTAGGAAGATTAACTGGATTAGCAATTGAAAAAATGAACGAAGAACTAAATGAAGTACATGAAAGAATTGCTAACTACATTAAGATATTAGGCGACCATAACTTATTAATCAATTTGATAATTGAAGAATTACAAGAAATTAAAAATGCTTATGGCGATGCTCGTAGAAGCGATATAAATTGAGATGAATTGAGCAGCATTGACAATGAAGATTTGATTCCAAAACGTGATATTGTTATAACATTAACAAATAATGGCTATATTAAAAGACTTGATATTGAGGAATATAGAGAACAAAGAAGAGGCGGAGTTGGTGTATCAACTACAAAAACATATAAAGATGATGCAATTCAAGATATATTAGTTGCAAATACCCACACGGACTTGTTAATATTTACAGATCTTGCCAAGGTTTATAGAATTAGAGGTCACGAAGTTCCTGTTGGCACCAAACAATCAAAGGGTATTCCTATTGTTAATGTAATTCCTTCTATTCAAAAAAATGAAAAAGTTATTAAAATTTTGCCAATAAATGAATACAAACAAAATGAATATTTACTAACAGCAACTAAGAAGGGGATTGTTAAAAAAACTCCAACAATTTTATTTGAAAGTATTAACAGAAACGGAAAAATTGCCTTTGCCATTCGTGATGATGATGACAAATTAATTGATGCATTGATTGTTAAAGACGAGGATGAAATCTTTATTGCTGCCTCAAATAATAAAATTTGCCGTTTTGATTCAAACGAAATCAGATCAATGGGAAGAATGGCCTATGGTGTTAAGGGAATTAAAATTGATGATGTTGAAGACAATGTTGTTTCTGTATCTTCTTCAAACCAAGGAAAATATATATTTAGTCTCGGAAAAGAAGGATATGGAAAATTGACACTTGCTGCAATGTTTAGAAAAACAAGACGTGGGGCAAGCGGGGTTTATTCATTAAATGAAGAAAAATCTGGCAAATTAATTTATGCTGCAACTGTTCACGGAGTTGAAGATCTGATAGTTTTAACAAGCGATGGTGTTGCAATTAGATTTTCTTTAAAAGATGTTGCAATAACCGGTAGAAATACTAAAGGTGTTAAATTAGTAAATCTAAAAGGAAGAACCACAAGCATTGTTGCGGCAACAAAAATTTTAGATGATGATTTAGAAGACAATGACAGAAAATTATCAGAAAATGAATTCAAAGAAATTGAATCAGATGCTAAAAATAACCTTAATCAAAAAAATGAATAATTATTAATAACAAAATAAACTATAATTATTATGTATGCGTTAAAATGCATACAGATTGTAGTTGCACAGAGAGGTTTTGAAAAATAATCTCTCTTTTTTTATATTTTAAAAACCTCCTTATAAAAGGAGATTTTTAAAATAAATTAAATGTTTAATTTTCTGGGTTTTGTTTTAATTCTGTAAGAAATTTCTCTAATTCTTTGTTTAATTCAATCGATTTCAATAGTCAAGGAGTTTTATCAACTCCCTTAAAATATTCAAACATTCCTTCAGGTGCATCTTTAAGTCCAAATCATTCAACTCATTTGTTGATAGATTCTAATTGATTTGAATTGTTGGGTAATTTTAACGTTAATTCGTCTAATTTTGTTTTTCATTCAGAAATTTTTGAGGCATATTTTCCACTTTGTTCTAAGCGTTTTATAGTATCATTAACTTCTTTGTATATTGATTTATTTGGATCGATTCTGGTCATTGTCGTAGAATAAGGTCCGATCTTGCGAACTGTTGTAGTGGCTTTGTGTTCTTTAAAGTTTGATAAAATCTTTTTTCAAACAACATCGCAATAAAATCATAATCTTTTTTCAACTTCTGACATTGTTTTATTAAAGTTAGACACATATTCATCAAAGGTCATATTCTTAAATAATATTTTCTTTTCTTTTGTTTCGACTACAGTGTTTTTAGATTTGTCAATTTCGTATTGGGATTGTTCAAATTTAACAGCATTTTTAGCAAATTTATTATTTCATTCAGATGAGATACCCTCAAAATAAAGTCCATCAGTTTCTTTTAAAACCTCACCACGAATATATGGAGCAGTATTTCAATCGTGAAATAGTCAATTAATCCTATCTGTATTTATATTTCCATCAAATATTTTTATTAAATCTTTAACAAATTTTTCTTGTACTTCTTGTATATTATTTTCATATTTTGACGCTTCATCAATGGTTGCCAATTGACTAGAATTGTTTTTAATATTTGCTAATTCTTGCAATGCCTTTTCTTTTATTTCATTTAGTTGTTGTGTTACACCTAAAAAATCTGGATCTTTTACACCACTATCTAGCTTATTAAATTTTTGCAAACTATTATTTACATCATCTAGACTATTATCTATTAATAATTTTTTAGCATTAAGATATTCAATTGAAATTTCATTAATTACTTGTATTATTTCTTCTCTTGTATGTAGAGTACTAATATTATGTTTTCTAATAGATTATTCTATTCTTTCAATGTATGCATTGTATTTATCTCTAGGGCGCATTTTATTTTCGATATATTCCTTTGCTTCTACAATTAAAGAAATTAAGCTATCCCTTAATTTTTGCATATCATTTTCAACGAATTCTTTACTTTGTTTTAAAACCTCATTTAAATTAATATTTGCAGCTACTATTTCTGATTTATTAGAACTAATTGAAACGTTTCCTCGTCTTGAAACTTCTCTATTAAGATTTCTAACTATATTTGCATAATAATCAGATTTAACAACATCTTGGGCCAAAAATTCTTCAACTAATTTTCTAGTTTTTTGTAATTTTTCAAATTCTTGTTCTTTTGATTTTTTAAATTTGATCAATATATCTTCAACTTCAGAAATTTTAAAAGTTAATGATTTATTTAAATTATTTATTTCGTCTATATTATTTTTGTTATAAACCTCTAATGCATTAATAATTTGCAAATTTAAATCTTCCTTAGTTTTTTGAATATCATCATCATTATCAATTAATTTATTTTCCAATATCCTCATAGCATGAATAATATTTTCCAACTGTTTCTTTGCTGCAAAAAGTAGTTTTTGCATGCTTTGCTTTTGAATTTTTGCATTTAATGAATCAATCGAACTTTGAATCTTAAAAATTTCTTGATTAATATTATCAATTGGCCATTCAATATTGATCATTTTGGAATTATCAAAGACATTTTGTTCTTCAATTGAATAAATTCCTTGAATGTTTTTATTTTCTAGCAATAATTGCAATTCTTCTTGAAGAATTTTAAATTTTTTGAAGAGTGATTCTTTTTGTGTCTTTTTATCCACTTTATTTTGACACGATCTTATTGCTACGGTAGCGATGGCAGCTATTGATAATCCGCCACAAAAAATTGCTAATGGAAGAACGATCTTTTTGCTTTTATTGTTCATTTTTTTTCTCCATTTTTTTATTATTTTTAATATTTGTAAAATTATTTTATGTTATTTAGTGCATAAGAATATTTTTATATTAAAATAGCAGGAGTGAGATCATATAAATAGAAGAAAAGGTTTATTTTTTAAAAAATACATATATATTAAGCAAATAATTTATTATCGATTAATATGAATATATTTTACTTGTATAAAATAATATTCTTTGTCTAATCATAATTATTATTGGATAGAGTATGAAAATAACTGCGGTATATATTGGTATTTCAATAAGAGTTTTTGCTATTATAGGAATCATAAATATTGGATAATAAGTTTTGTAATCTCAATTACCTGATCTAAATCTATTATGATAATTTATATATGCAAATGGTCCTCAAAATCATCTACACAACATTAGGGTAAAAAACACAACCAACAAAATGCTAAATAAAAGTACAACATTGCTTTTTACAGAAAATTTTCTATTTTTTATAAATAAAGTTAAAAAGGTTATAGAAGATATTCAAATAAATGCTAATCCCGCAATAGCAATAGATAAAACAATTCTGATTGGTATTAATTTGGTGCGTTTTACTGCTGTTTCATTTATTGGCAAATTGTTACCGTGAATTGCTAAAACTATTACAAAAATTGCTGTTATTATGCTTAAAAAACTAAATCCAATTATTCATGTAATCTTTTGTTTAGCATACATTAATCTTAATAATCAACCACTTATTAAAGCAATGAAAACAGGCACTAATGCATATTCAATCATTCATGTGCTTATTCCTCTAATAACCTGGTTTAGCGTATCACATAGTATACCTAAAAAGGCACCTTTTCATGGTCCTAACGCTAAGCCAAAAATAATGAATACTGCATAAGTAATATTAATATTCATTATTCCTTTAAAAACAAATCTTTCTAAGATAGCAGCAATTATATAAATAGCTAAAAGTAAAGCAAATAAAGAAATTTCAAAAACTGTTAATTTATGTTGTTTTGTTTTTTTAATACGTCTAATTTTTTGAATAAATTCAAACATGTTTCTCCTAAAATAAACCCTAAAAGGGTTTTAAATTTTGATAAATTATATTTTTAAATACGAGTTTATATATACGAGAACAGTTTTTTAAATCATAACTATTAAATTTTAACTATTTTTTAAAAAAATAAACAAAAATAAAAATTTCGGTTTCTTGTCCGAAATTTTTAAGTCTTATTTCAACACTAATTCTGTCAATGTTTTTGTCATTGCAAACATTGGTTTTTCATCAATATCATTAGTTCCCGCAACATCTAAATGAATATATTCAATTCCTTTTGTAAATTCTTTTAAGAACATTGCTGCTGAACAAGAACCTGCATGTCCAGTATAGTCTGTATTCTTCAAATCCGCAACAATTGAACCTTTCATAAATTCTTCATAGTCTGAATCAAAAGGCATTCTTCATATCAACTCATGTTGAACGGTTGCTGCATCGCTAATTTCTTTTCATGCTTTTTCAGTTGTTGATCAAATGCCTGTATATGTGTCTCCTAGAGCTGCAACCATTGCACCAGTTAATGTAGCAACATCAATTAATCTTGTTGCTTTTAACACATCAGCTCCATAGCACAAGCCATCAGCCATAACTAATCTACCTTCAGCGTCAGTATTATTTATTTCAACAGTCTTTCCACTCATAGAAGTCCAAACTGAATCAGGCAATGAAGCATCTCCATTAACTCTATTGTCAGTAATGCACATAATTGCTGATACATTTGTTTTTGGTTTTAATTGGGCAACAGATTTCAAAACGGATGCAACTATTGCAGAACCTGACATGTCATATTTCATTCCTAACATGTTGCGGCCTGTTTTAATGTTGTAACCGCCAGAGTCAAAGGTAATTCCTTTACCAATTAAAACTGTTTTTTGATTTGAATCAGGATTTCCTTTATATTCAATTATTACAACTCTAGGTTCATAAACTGAACCTCTATTCACAGATAGTAATAATCCCATACCCAATTTTTCAATTTCTTTCTTTTCAAGAACTTTAACTGATAGGTTTTTATATTCTTTAAAGTCTTCAACAACTATTTTAGCTAATTGTTCAGAATTTAATTCATTAGGAGGGGTAATACCCAAATTTCTTGCTCAGTTTTGAGATTCAATTAATATTTTTGCTTTATTGAATGAGTCAAGTACTGATTGAGACGCTTGTTCTATAAACAATTCTATGTTGTTTGGATTTTCTTTATATGTAAATGTCTTTTTATTGTATAACTTTGCAGCTGAGAAATTTATTCCTTTTGTTAATGCATCAACTACATCATTAATTGTTAAATTTTCTGTTACAAAAGAAGAAAGATCAATTTGATAATCGTAGGCAGCATTCAATGCTAAATCTTGTGCCAATGAATATAAAGAATTGTATTGTAATTCTTGTTTTTTGCCTAGATAAATATACGAAACTTTTTTATCAATAAAATCTGTTATTGCTTCTGTTTCTTTAACTAAAAATTCTGGCAAAATTTCATCTTCAAATATTGCTTTCAATAACAAATGATCATTGCGCTTTGTTGATAATTGTTTAATTAATTGCATACTATTCTCCTTATTATTTTTGATTTGCTGCTAATTCAACAAAGGTAGAAACTAAAATTCCTAGTCCTACTCCTTTATTGTCAGCGGTTCCTGCAATATCGCAATGAATATAGTCAACATTATTTGTAAAATGTTTTAAGAACATTGCTGCTGTATTACAATCTGATTTTTCACTGTTGTTGTAATTATTAAGATCAGCTACTTTAGATTCTTTATTTGGTTTATCATAATCATCATGTAGAGGCATTCTTCAAACCTTTTCATGAGCAATTTTTGCCGCTTTGTTGAAATCTTCTCATCTTTGTTCAGAAGTTGATCAAATTCCTGAATACGTCTTTCCTAATGCTCTTTCCATCGAACCGGTTAAAGTTGCGGCATCAATCAATAATGAAGCATTCAATTTCGAGGCACCGTAGTATAAACCATCAGCCAACACTAATCTTCCTTCAGCATCAGTATCAGTAATTTCAACTGTTAAACCACTCATTGATTTGATTACAGATTCAGGCATTGTTGGGTGTGTATCTATTGCGTTATCTGTAATCATCATTATTGCTGCTACGTTTGCTTTTAATTTTAGTTGAGCAATTGCTTTAACTGCATATGCAACAATAACAGAACCAGACATGTCAAATTTCATGCCATCCATGTAGTATCCTTTGGTGTTATATCCTCCGGTATCAAATGTAATTCCTTTTCCTACATAAACATATTTTTCTTTACTTTCAGGATTGCCATTATATTCAGCAATCAAAACTCTTGGTTCATAAGAAGAACCCGAATTTACTGCCAACATAAGTCCCATGTTATTTTTCTTGATGTTATCACGATCTAAAACTTTTATTGATAGTCCAGGAATATTTTCTAAATCTTCTTTTACTACCTTAGCGATATATTCACTAGTTGCAACATTAGGGGGTGTCATTTGCAAACTACGGGCTTTATTTGTTGCTTCAAGAATTATTGACAATCTTTCAACTAATTCTTCATACGATTTATCAGCATAAAACAATGTTATTGTCTTTTCTTGTTTGTTTTCTTTGGTTTTTGCTGAATAAAGATTTGCATTTTTAAATGCTAATTTAGATACAAATGCTCTAAAAACTTCTTCTGTTGACACAACATTTTCTTTAACAAAAGAAGCAATATTAATTTGATAATTTCTTCTATTTGAACTTAGAATTTTGTCAACTACTTTTAAAAAACTAAAGTAGTTATCTACAGTTTTTGGTATGTAAATAAATGCTTCATTTTTTTCAAAATCTTCTGTAATATGATTATCTTTCTTGGCAATATTTAAACTAGCGTTTGAGCCTTCATAAACTGCTGTTAAAAGCATATCTTTATTACGTTTAGTTTCATAATAAATAGAATTCATTTTATTCCTTTCTAATTTTTAAATAAATATCTTTTAGATAATTAAATTATAATGATTAATATTTTTTAGAACGTAAAAACATAAAATAAAGAGAAAATTTTTAATATTTTTAATTAGATTAAAAATAATAAAAAATTAGCCTTACTAAAAGCTAATTTTATTATTTAATAGTTTTAAGTTGATTAATAACTAAATCTATGCTAAATAAGATTGCGTCGCATTGTTGATCATTTAGTTTTGCAATTTCTTCATCTTTTATCATTTTTCCATATTGGTTCATAATTTCACCGGCAGATGGTATCTTTTTTGCCATATCAAGTAATTGCTTTATTTTTGATTTAACTTGTTGTTGCTTTGATGTCAATTTTGAACACGATATTGCTACTATTGGTAATGAAATTAAAGAACTTGCTCCTAAAATAGACAAAATTATTTTTTTACTCTTCTTCATAGAATATCCTTTATCTTTGTTTAATGAATCGTTAGATATGATAATTATAAATATAAATTTAATTATTTTTTAGTAGATCAATCGATTTCATCTTTTGATAATGATTTAAGTTTAAAATTTATTTTTTTAAAAACATTTGAGCCAAAAAATCCTTTATATGCAGCAAGAGGTGAAGGATGTGGCGCATAAACTATTATTTGTTTAGACATATCAATGTTTTTTATAAAATCTTTGGCGTTATTCCCTAAAATTAAATAAATAATATTGCTATAACACGAATTAATTTCTGTTAATAAATTTTGATTGAATAGTTCTCAACCTAAATTCTTATGTGCCAATGTCTTTCCTTCAATGTTTGTTAAAATTGAATTTAAAAGTAATACACCTTGGTCTTTTCAATAATTTAGCGAAAAAGTTTCAAATGTTGAATTTGGATATTCATTTTTTATTTCAATAAATATATTTTTTAAAGATGCCGGGCATTTTGTTGCATTGCTTGAAAAACACATACCATCTGCGTCACCTTTGTTGGGATAAGGATCTTGGCCAATAATAATAACCTTTAGGTTGTCAAAATCGAAATTTTTAAATGCAAAAAATACTCTGTTTGGCTTTGGCGTTACATTAGGCAACATAGTTAATTTTTTTAAGACTTCAAAATATGGTTTTGAAGTTTCTTTTTTTAAAAATTCTAAAAAAGCAAATTTTGACATTTTTAAACTCTTACTCTTTTTTTCCCTTCAAATTGATATTCTATTAAGTACAAATATCCATCATTAAATTTTATTTTCAATGGGGTACTAATTTTTGTTGTTGAAGCCCGGAAGATTTTTAATCTTTTATTTTGAAAAATTAAATATGCACCTGGTTCATCATTAAAAGCTCTGATTTTGTTTACAACACTTGAAAGATTTTCGCTTTGATCAATCCTTTCCATACCATTTAATATCTTAGGAGCAAATGTTACTTTAGATTCATCCTGAGCCACTCGATTATATTGGCCGTTGTAAATTTCAAACAATCAATTACCAAGATTTTGCGAGGCCGCATTTGCTAATTTATCAAATACTTCAATTGCTGTATCTGTAGAATCAATTTTCACTTTCGAGGATTCTAGGATATCTCCTGCATCCATTTGTTTTACCATTTCAATTAATGTTATTCCTGTTTCAATATCACCATTGAGTAATGCATATTGCACAGGCGCAGCTCCACGATATTTTTCAAGCAAACTTCCGTGAACGTTCAAGGGCATTTTTTTAGGTAATTTTAATATTGAATCAGGTATAAATTGTCCAAATGAAGCTGTGATAAAAAAGTCAAAATCCATTGCGCTAAGTTCGGGCAAAATTTCTTTTATTTTTTGAGGTTGAAAAACTTTTATATCATACTGTTTGGCAAGTTTAAAAATTTCTGTTTCAATTACATTTTTGTTTCGATCCAATTTTCTATTTGGCTGAGAAATAAATGCCAAAACATTGAACCTTTCATCATTTAAAAGAGGTTCAAAAGTTTTAGCAGAAAATTGTCCAGTTCCCGCTAGAACTAAATTAATTTTGTTATTTATATTTTTCATATCAAAATTATAAAATAAATTATTTTTTATAAACTTATTATCTTGTATTTCAAGCAAATGTTTTTATTGACAATAAACTCAATATTACAATTCAAATTCAAGGCAATATGAAGTTCAATAAATTTTCTGTTTTTGAACCTATTTTTGTTAAACTATCATTGCTTAAAACGTTATTTGAAAATTGAATGTAACTACTGTCTAAAAATTTAAAATAGTTTGCAAATTCATTATTCTTTAACAATTGGTCCAAACCATTTTGATTGAATGTTTCAGCAATGGTTGAGTATTTTAAAATTATAGGTTGATAAACATTTAAAATATTTAGTGTTTTATATTCGTTGTTAATATTAAATATATTATATTGACTATATTTTATTCCAATTGGTGTATTGGCTGGCAATGCATTATTATTTGCTAGAAATAAATCTTTTTGTTGATTCAAGAATTTCAAAATTGTTAACCTTGCAATTAGATCTTTGCTATTTGCTTCTTTCAATAAATTTGCATATTTTGTAACACCTTCAATGTATTTTTGAAGTTCTTCAATTGTATAGATTTTATTGCTTGAATCAGCACTTGAAAAAGTCTTTAATAAATCTATATTTTTTATTTCATTTCCGATGTTAAGAGTTGCCTTTATTGAAGCAATATTATACTCATTTGATAAGGATGCTTGACCTTGAAAAGAAATTGTGTTTTGAATTATTGCACTCTTAAAGGGCGTTAAGTAGGAAACGTATCACATTCCCTTTATATTTGAAATTTGAGAAATAGGCAACACTTGACCCGATAAGAACATTGTGATTATTAATAGCGAGCAACCGATTGCTTGAATAGTTAAGACTGATTTTGAAACGCTTATAATGAACAATCCAACAGATATACTTACAAAAATTATAATGACAAAGCTAAATATGTAACCTGGTCAAAATATCCTTGAAAACATCTCTTTAATACTACTTCCGTATAAAGTTATATTTAGACTTGCTTCCCCTATTTTTATATTATTAATTGTTCCTAAAGAACGACCGGTTTTAAAATATTCGCAAAAAAACATTAAAGCAACCAGCATTGTTCAAAGGCCAGAACAAATCATTATAATTAAATAAAATATTGCTGTATATGCCAAAAACGATTTAGGCTTAATGCTAGAGGCGCCAATTCTTTTTAAAATTGTTGATTTTTTAAACTCAAATATTGCTGTTGGCAGTGAAATGCAGGCTATTGCCACCGGCCCAATGGTTATTGTACTTCCCAAAATCATTTCATAACCAAATATCGTTCCTAAAATAGCAACGAAAATAATTGGATAACCGAATGCAAAAAATGGTCCTACAAATGATTTTCAAAAATGTTTATTAATAATTGCTAATATTCCTAAAAACTTATTTTTGTTAGCCTCGGTATTATTGTTTTTTTTAATAATTTCTTTATTTTTCATAATTTTAAATATATTCCTTAAGACATTGAGATATTGATCCATATTTTTCAATTATTTCGCTTTTCAAAGCATCAAGGACAATTCTACCTTTATTCATAATAATAATTCTATCTGCCAGAATTTCAATTTCATTTATATCGTGAGATATTAAAATCAAAGTACAATCAATTTCTTTGATATAGTCATTCAAAAATGATACCAATCTTGTTTTTATTTTTATGTCAAGACCAGTGCTTAATTCATCTAATATTAACAACTTTGGTTTATTAATTATTGCAAGCAATGCATTAAGTCTTTGATGTTGGCCCCCGCTTAAGGAAGAAGCTTTATTATTTATAAATTCATCAACCCCAAAAATCTTTATAAGTTTTTGCAATTCTTCTGGTGAACTTTTATTGCCATAGATTTTGTTCATTGAATAGATGACATCATTAACCGATAAACCTTGAGGATATGAAGAATCCTGAAATTGAATTCCTATGTTTGTTTGTGCACTTTCATTATGATTTGATCCATCATTAAAATAATATTCAATATTGCCTGAGGTAGGTTTTAAAATTCCTATAAGCATTTCAACTGTTGTAGTTTTTCCGGCTCCATTTCCACCTAATAGAGCAATGTTTTGATTCTTATAAATTTCGAGATTCAAATTATCTACAACTTTTATTTTTTCTTTTTTTATTTTGAATTCTTTTGTCAAATTTTTAATTTTCACCAACTTTTTTAAATTGTATTGGCGCATTTCAATTGAATTTGCTTTAATTGTGTTTTTTTCATGATCTTCTAAATTACTTAAAAAATTGTCTATTATTTCTAGACTTTTATTTTTACAAACTTTTTCATTTTCCACTATTTTAACTCCTTATTTTATATATTTCCCTTTTTGCCTAAATTATATCAATAACTTAAAGAATGAAAACAAAAACTCGTAATCAAAGAATTACGAGTCAATGTTTTTATGCTTTTTTTGTTTTGCTAAAAATTTTTGGTTCTGTATGTTTAAATATTCGTTTAAAATTGGGTATATGTTTAACAACTATTATTAGCGAAGCCAATATTAAAATAATTACATGTGCGGGATATATAGCATTTTGTTGCATATAACCAAATATTGTTCCTGCATAAAATATATAAATTAAACCTAAATAAGGAATTATTCCGCAGCTTATTGATGAAGCAAGTGAAACATATTTTGTTGAAAAAACTAGTATTACATATATTGCTACAAAAATTAAAAATAATAGAAAATTATATGCAAGCATTATTCCAAAAAAGCAAGCTACTCCTTTGCCGCCTTTAAATTTAAAATAAATTGGAAAGACGTGTCCAATTACTGCACCAAGCCCTGCTAGCATTGGAAAAATATATTCAAGATTTCTTAGATATTTTTTAATTAAAAATGCAATTATTGTTGCTAAAAAGGCTTTAAAAATGTCAAAAGCAAAAACTAATGCTGCAAATTTTATTCCAAAATTTCTTAATGCGTTTGTTGCTCCAGGATTTTGCGAGCCAATTTTTCTTATATCATTCCCTCTTAATTTAGTTATTATTATTGCCAAATTAAAAGAGCCAATTAAATATCCTAATATTAAAAATATTATATTTAATCACACATATTCACCATAAAACATAAATAAATGATACACACAAGAGAAATTATTTTATAAAAACTTACAAAAAAAATAGCAAAACTCTATTTTTGTTTGCTATTTTTCGGTCTTTGTTTCAATGTTAATTATATTGCTTGCAACAACATCACTACCAACTAAAATATCATAATGAGCAGATATCTTGTTTCCTTCAATAAAAACATCCTTTAAATAAAAATCTAGAGAAAATTCTTGAATTTTATTGCTTACTCTTTTGTTTTTTTGTAACTCAATATATGAACCAGAATATTTTATTTTTAAAGTATCACTATTGACCAATAAATCACATTCCTGATTATTATTATCAATAAAATTTATATGAATAAATTCATCAACAACTTCTTCGGTATATGGAACAAATTCTGTTTCTATCGTTTTAGAGGGTTCATTATTGAATTCGTTTTTTGTAATTAAACTATATTTTAATAATTCCATTTAATCTCTTAAAAACTAATTCAGCCCCAAAAAGATAGATTGCCTTTGCAAGTTCAGGGCCATGTTCTTCAAATGTTGTTGCTTTTCTAATTGGCATGAACAAATCTCTTCCCTTAACATTCAAGATAGTTTTTGTTTCATCAATTGCTTTTTGTATATTTTCTATATTGAATTCTCTTGAAATTAACTCTTTATAAAAAACTTTTACAACATCTAATCTTTCTAACTCTAAGTCGCAATTTTCTTTTGGGTTTAAATAAATTTCTAAATCTTTCTTTAATGTTGAAATCGTTGGACTTGATTGTTTATAAGTTTCAACAAACAATTCATTTCATTCATGTTCTTTTGGAGAGTTTATTTTTTTAATTAACTCTTCATTAGAAATATTTTTCATATATTGCTTTGCGAATCAATCCATTTTTATAATGTCAAATTTAGATGGTGATTTGCTCAATCTTTCTGGATCAAATTTTGCAATTAATTCTTCTCTATTCATTAATTCTTGCTTGTCTCCAGAAGTTCAACCTAACAAGGCTAAAAAATTCATTATTGCTGCTGCAGGATAGCCTTCATTTTTATAGTCTTCAATAAATTGTTTTAAAGTTTTATCTCTTTTTGAAAGCTTTTTTCCTTCCATATTCGTAATAATAGTTAAATGTCCAAATAATGGAGGTTCTCATCCGAATGCATCGTATATTGCTAATTGCTTTGGTGTGTTTGTGATGTGTTCTTCGCCTCTTAGTACATGTGAAATTTCCATTTGGTGGTCATCAACAACAACTGCAAAATTATATGTTGGATAACCATCACTTTTCATAATAACAAAATCTCCTATATCATCACTATTAACTGAGATTTTGCCTCTTACTAAGTCATTCCAAGCATAATCTTTATTTGCTGGCAATTTTAATCTTATTGAATATTCTTTAGCTTCTTCTCTTCTTTGCTTTTCTTCATTAGAAATTTGCAATCAATTTGGATTATAACGAAATGACGCAACGCCCGCTTCCTCTGATTGTTTATGTTGTATTGCTAATTCTTCCTCTGAATCGTATGCTTTATAAGCGTGTCCATTTTGAATTAGTTCTTCAACAAGTTTTTGATAAACTGGTAATTTTTCGCTTTGACGATATGCGCCGTATTTTGAATTAGGTTTTAAAGGACTTTCATCAGGGATGATTCCAAGTCACGCTAAATTATTTAATTGGCTTTCTTCACCTCCGACAACGTTTCTAGCAACGTCAGTATCTTCTAGCCTAAAAACAAAAGTACCATTGAAATGTTTTGCAAATAAATAGTTAAATAACGCAGTTCTTGCACCACCAATGTGCAAATACCCAGTTGGGCTCGGTGCATATCTTGTTCTTATAGTTTTCATAATCTACCTTCTTTTATGACTTTTCAAAAATAGCAGTTATGGGTTCTGAAGCATTGCTTCAAAACGCATCCTTATTTCCCTTCATAATTCCTTTATAAAAAGGTCAAGGTGCTTCCTCGATAGAAGTATTAACATTTCTATTTTTTCCTTTAGCTAACAATGATTCAATACTTGGATCTAGGAAATCTGATTTTCAATTAGGGGCTGCTTCGTTTTGTTTTAAAGTTCAATATAAAACTAAAAATAATGCTCTTGGATTATCTCGATAAGGTCAGTATCTAGAATCATTTTTTGTAGCAGGAAAATAATTAATTAATTCCTCTCATGTAAAAGAGAAAACTCTAAAATCATAATTTGGAGAAATATTATTAAAATCTAAAAATCTGCTAAAAAATTCTCTATAAACTCTTTGCACTGGTTTTCCGTCAATTACTTCGAGTTCATTATTCAATACTTCTTTCAATCTTGGGTATCCATTTTCTTTGTCATAAATGTAGTTTCCATTAGCATCTCTATCGAATTCTATTTCACCATTTTCCAATTTTTTACCCTTAAATTTTAATAAATTTCCGTTGGCATCTCTAAAGTAAATTGGGTTTCCTTTTTCATCTTTTAAATTTTTTGTTAATTTGTACAAGGAATCTCTTGGCGATATCGGGTTACTTACTGAATAATTATTTTTATTAAAATCAAGTGCAGAACCCTTAACGTGATAACCATATTTTTCAAATGATTCTTTCGCTTTTGAATTGAGAGTTATGGTTGCTGTTTTATTACAGCTTGCCGCAACTAGTGGCAATGCAAACACAGGAGCAACACTTCCTATACAAAGTAAAAATTTTGTCATAATTGCTTGTTTTTTCATGTTTAAAATTATACAAGTTTTTTTAATATATTAATAACCATAAAAATAAAGTTTCTCTAACTTTTCGCCATTTCAAAAAAAATAAACTTTTTCAATCAATAATTTAAATATGTGAATAAATAAACAAGATAGTGTTAAAGATTGTGCAATTTATACATTGCAAGCAATGATTTGCCACTTTTTTAAATATAAGGCAGATATAAATTTTTTAAAGAAAAATACGATATACGACGAAGAAGGAGTGTCGCTATATTCATTTAAGAAAATTTCTGATTTTGTGGGGTTTGATACTAATATTTACAAAGTAGATTTTTTAAGTTTTATTTCAAATTTGAATGATGTTTCATTTCCGTTTGTCACAATATTGAAAAATAATAATTTTTTGCATTATACAATTGTTAAAAGAATTGATAAAGCCTTTGTTTATCTATGTGATTCTGAATTTTTGAATTATAGATTAGAAATTAATGAATTTAAAAATAAATTCATAAATGTTGTAGTTATATTAAGCAAAAAATTTAAAACCCCAAATTGCACAAAAATTAACTATAATCTATATCTAGCACAAAACACAAAGTTAACAATAATTAGTATATTTTTGGCAATTTTATTCAATGCTCTTATGCTTTCTTCTTCATTTTATATTAAATATGTTTTAAGCGTTTTATGATCGGAAAACGCTAATAATAGATTGCTGTCAATATTAATTATTTTTAGTTGAATTGCAATATTAAAGGCAATTACTTGATTATTTAAAAAGATTATTATTAATAAGCTAACTTTTTATTATGAAAAAAAGTTATATTTGTTAGTCAAAGAAAGATTAAAATTTGCAAGTCAAAAAGACCTGCAAAAAATTAACCAAAATGATTTTATGGCAAGAATATTAAGTATTTCAACTATTGCAGAATATAAGGCGGGTTTATTTTTGTTTTTATTTGAAAATATAATTATTTTTATTAGTTCAACATTGCTTTTGGGTCTGTTTAACTATAGAATATTATTAATTTGCTTATTAGGCATTTCAATAACCTTTGCAATCTCGTTATTCAGTTTAAAATATCAATCAAAAAAAGCAACAGAATTAATAAATTTAAAGATAAAATCAATTAAAATATTCAATAATTATTTTTTAAATTATGAATATAGGACGAATGATTTAAATAATTCGAATTCTAAATTATTTCAATCGTTATTTAACACTCAATATAATCTAGGAATATTCAATGAAACAAAAGATTCATTTATTACATTTATTTGAGATTTTATTCAATTAACCATTATATTTACTGGCGCTTGATTATTTATAAAAAATAAAGATAATTTTACAAATATTACATTATATGGATCAATATCAATTTTTTTGAATTCCCCTATTGTAAATTTAGTCTTCCTGATTTCAAATTCTTCAATAAATAAACAAGGAATAATAAGAATAAAATATTTATTAGAAATCACACAAAACAAGGATGGAAATATATCGTTCAAACCCCAGAAGCAAAATAAAATAAACATATTAAATGCTTCAATAAATATTGGTTCAAAAAACGTTATAAAAAATCTTAATTTAACAATTAAAAACAATCTAGAAATATCAGGGAAAAATGGAGTTGGTAAAAGTTTGATTTTGAATGCTATATTTAAAGTTTATTGATTGAATCAAGGCCACATTTTTGTCAATAATGTAAATTTAAAGCATATTAGTCAAAAGTCATGATTAGATGCCTGCTTTTTAAATAAAAATAATGAATATTTTTGCTATGAAAATCTAATGGATTGCATATGTAAGAAAGACAATATAAAGGAAAATATTTTTCTTAAAAATTTCAAAAAATATAATTTAAATAACTTGTTTTTAGATATGAACTTACCTCTTAATAAATATATTGAAAATGGAGGAAATAACCTATCATCCGGCCAGAAGGAATTCATAATTATAATGCGACTTTTTTGCCAGAAATATTATTTTATTCTTTTGGATGAGGCTTTTGAAAACATTACAAATGAAATTTTTCTTAAATTAAAATTTTTAATTTTAAACTACCAAGATAGGGCTATTTTTATTGAAATTAGCCATAACAAAAAAATTGTTGCAAACAAGCGAAAAATTTTCGCTTTAAAAAAATAAATATTTTTTTGTTTTTATTTAGTTTAAAAAAGAATATTATCTTTATAATTAATAAAATATGAATAGAATTAATTTTAAAAACCAAAGCTTTCATCGTTTTAATTTTATGAAAGATTTCTTAGATATTTTAGAAGAATCAAAAATAGAATTTGGTGCAAAAAAATCACTTTGTGTTGATGTAATGTTTGTAACTGCTAAAAAGATGAAAAAGTTAAATAACTTACACAGACAAAAAAACTATACAACTGATATTTTAAGTTTCCCACTAGATAGTCAAGAAGAATTAAATTTTTTGGATGAAATTGAATTAGGACAAATTATTATTTCTCCATGGAAAATAAAAAAACAAGCCAAGGAGTTCAATCATAGTTTAAGACGAGAATTTTGTTATATTTTTGCTCATGGGATTGCTCACCTATTTGGTTTTGACCACATTACTGAAGAAGAAGCAAAAATAATGAATGGGCATGTCGATAATATAATGAATAAATTAAACATAAAGAGAATATAAAAATTGGAGAAAAATGAAAAAAGTTTGCAATGTATGCTTAATTGGGCGCCCAAATGTTGGAAAAAGCACGTTTTTAAATAATGTGCTAAATTTTAACCTATCAATAATTACGGATAAGCCCCAAACAACAAAAGATAACATTAGAGGAATATACAATGATAAAGACTACCAAATCATTTTTATTGATACTCCAGGAATTCACAAAGCAGAAAATTTATTATCTGAAAGATTAAATAGTAAATCATATGAAGCTATTGAAAATTCTGATGTTGTTTTGTTTTTAACTCCCGCTAATGAAGCAATCGGAACAGGAGATAAATTCATTATTGAAAAAATAAACGAAACTAATAATGAAAATAAAATTGCGGTCATTTCAAAAATTGATTTATTAAATACAAAAGAAGAAGCAGACAAAAAAGCAGAAGAATTAAAAAAACTTGGATTTGAAAAGGTTTTTGGCATTGGACAAAATTTGCCTACTTCTTATTCAGATTTGATCAATGAAATTAAAACCTATTGCTATGAAGATGAACCTTTGTACCCTGATGATCAAATTGGGGATATAACATTAAGGTTTATGGCAAAAGAAATAATTAGAGAAATTGCTATAAACAATTTAAAATTTGAATTGCCACACTCAATTGCGGTTGAGATCGAACAATTTGACGATAAAAACGAAGATGAAGATTACAAGATCTATGCTTTAATTTATGTTAAAAAGAAGTCTCAGAAACCTATTTTAATCGGAAAAGACGGCCAAATGATTAAAAAAATCTCTATTGATTCACGACATAAAATGGAAAAAGTTTTTGATCACAAAGTCTTTTTAAATATTAAGGTAAAAATTAGCGAAGATTGAGTAGATAATGAATTCAAAATAAAACAGTTAGGATATTAAGGTGAAACGATTAACTACACATAGAACGAGGGTTATTTTTATAGCAATAATTGCCTTACTAACACTTGGATTATTTTTAATAATTTTATTTTTTAAAATTAGCTCCCCATTTAAAGTAAGTATATATAACTACGAATCATACTTAGATAAGAAAATAATTACCAAATTAAAACAAAATTATTCATATCACGCCTTTACAAATCTAGATGAGTTTACGAGAGCAATAAATAACGAAAAAGCCGTTGCCGGAGTTGGAAGTGATTACCAAATTGCTCAACTAATTCTTGAAAAAAAATTAAGAAAAATTAACTTTGCAAAATTATTTAATGATTCAAAATTTAATGATTCAACCCAAATAAAGAAGCTTTATCCAGAAATTGTTAATAAACATTTTGATCTATTTGAAAAATTTATCATTAAAAAAATAAGACAAATAAATCCTAAAAACATAAAGCAAACTGATTGGCAAAATATTAGTCACTCCAAATTCTTGCCTTTTATTTATTATGACGAACATAACAATATTTTGGGTTTTGAAATTGACCAAAACCAAGGAGTTGACCACTTTTACGAATTTTTAATCCCTTATTTTATAATGGATAAAATGGTTGTTTATAATACAGATAAAAGTCCAAATAAATTTACTGAAAGAAGCAATTTAAAAGATAATGAAAACTTTAACGATTTAGAAAGTTTAAATTCTTGAAAAGATATCATTAAGACGCTTGTTTCTAAATACAAAAACCCTAGAGTATATTGAACAAATTGATTTCAAGATAATGCTATGATTGGCCAATTTTATGGTTATGAATCTCTTAACAAATTAAAATATTTTTCTAAGCAAAACGAATGAGCAGATATAAATCACGAAAATTTTAAAGAGATTATTAACGATTGGGTTTCTTTAGTTAAGGATGCAACAGGAGAAAGTATAAAAAATTCTAAAACAAATAAATTAGCCACTGATGGACAAGAATTAGTTAGTTCAATAATAGAGCCGATTAATGGCAAGGCAGATGTTTCAATTATGTATAACGGAGATGCCTTAGATGCATATTATGGGGAAGACAATTTTAAAAAATTAGGTAATATTCCGCATATTGGTTTTAAAAGACCAAAAAATACTTACATGAATATTGATGCTTGAATAATTTCAAGAGAAACATCTAAAAATGATGCCGATAATTTATTAAAAATATTATATGAAAATGTTATAAAAGGAGCTGAATATTCACTCCAAGATATTGAAACAAAATATTTGAAAGAAGTGTTGAATCTAATATCAAAAGACAACAAATTAAAAAAAGATGAAATTGTTAATGAGCTATTTGCAGACAAAAACATGTCTATGCCCAAAAAAATTAAAGACATTAAAAAAGATTTTTTTGCAGAATATTTTGACTATTTCAGAGAAGCATTTGAAACAATGAATTTACCCGTTGTAAACAATTTTCATTCAATAAATTACACACCAGGCTTTAAAAATGTAAAAAATTTTATTGAAAAATGGTACTTTTTGAATAAAAATCAGGAAGTTGATAAAACTGCTTTAGAGATATTCAATCCCGAATTTAATAAAAAAATCAATCACAGAATTTACCAACCACTTGATTTAAATTTAAAAACAAAAATAATAGATTATTACTTTCAAAAAACAAAATCATAAATTACTTAATTTCATGTTAGGAGAAAAATGGAAAATAAAATTAAAACACCAAACAAAAATGAAGAACCAATAATCCAATTAGTAGACGTTGTTAAAGAATTTGAAGATAAAACAGTTTTAGATAATGTTAACTTGTCAATAAATAAAGGTGAATTTATAACATTATTAGGTCCTTCGGGTTCAGGAAAAACAACCATATTGAGAATTATTGGTGGTTTTGAATGAGTTACCAGAGGTGAGGTTAAATTTTTTGGCAAAGATATCAAAGACTTAAGTCCACATAAAAGGGATGTTTCCACAATTTTTCAAGATTATGCATTATTCCCTCATTTAAATGTAGAAAACAACATTAAATACGGTCTTAGATTAAAAAGAATACCAAAAGATGATATCCCTAAAAGTTTTTACAATAAATTAAAATATCAACAGAAAAAATGAACAAAAAAAGCTTCTATCGAAATGAAAAAGCTTGATAAAACAATGGCGCAATATGAAAAAATTCTTGAAAACCAAAAACTTCCTGAAAAGAAACGGAACAAATACCAATCTTGAATTGATGACATGGATTTCAAATACTCATATTGAGAAAATTTTAAAGATTTAAAAACTGAAAATTATGAAAAAAGATATCTAACAAGAAAAATAACAAATAATGAAATGAACCAAGAAGTATCGGAAATCATGGATCTTGTTGGTTTGAAGGGAAATGAAAAGAAAAATGTTAATTTCCTATCTGGTGGTATGAAACAACGTGTTGCACTTGCTCGCTCGCTTGTTATTGAACCTGAAATTCTTCTACTAGATGAACCATTAAGTGCTTTAGATGCTAAAATTAGACAAAAAATGCAAACATTATTAAGGGATATTCAAAAAGAATTAAAAATTACGTTTATTTTTGTTACTCACGATAGAAATGAAGCATTGCAATTAAGTGATAGAATCGCCATTATTAGAGATGGTAAGGTTGAACAATTTGCTTCGCCAAAAGATTTATACGATTATCCAATAAACAAATGAGTAGCAAATTTTATAGGCGAATCTAATTTCTTTGATGCTGTATTTATTGATGAAAATAAAGTTAAATTTATGGGCAAAATTTTCGATACTATTCACAAGGAATTTGAGCCAGGAGAAAACCTTGATGCATTAATTAGACCTGAAGATGTCTATGTTACCAAAGTAAAAGCCAAATCTAAATTTATCGGCAAAATAATTAAATCAACATATAGTGGTAGTTACTACAATTATGAGGTTTTAGTAAAAAATAAAATAATAAAAATGGAAACATCAATAAACTATGAAGTAGGTAAAGAAGTATATTTAGATTGATACAAAGATTCAATTCATTTGATGAAAAAGGATATTAAATTTGCTAAGGAACTAGCAGAACAAAACGAGATTAACAATGGAAAAACAACTGAAATCAACTTCCTATAAAGAAATAGATAAAAAAACTAAAAAAAGTAGTAAATTTGCAAAATTCTTTTTAGGTGCAAACAAATTGCAACTTTCTTTGCTAATTCCTTATTTAGTATTTTCAATTTTATTTATTATTTTGCCATTAATTTTGATATTTATTTATGCATTCAAACCACTAGAAGGACAAGGCCATCTTGCAAACTGAGAAATAGCAGGTAAATCATCAACCTGAATGATTATTCTTAGATCAATTGGAACCGGAATATGTGCAGCGTTTTTTGCATTAATCATTGGTTTTCCATATGCCTATATTATTGCAAGAAGTAAATCAATGATATTTAGAACTTTAGGATTAACATTGATTCTAAGCCCGCTTGCAATATTCACAATTTCAAAAGCTTTAGCAGTTAGAGGTTTATTTTCGGCTATATTTGACGAAAATGAATTAAATAATAATTTCTTTCTAATCTTTGGAATGGTGTATTTGTATATCCCTTTTATGGTAATGCCATTATATCAAGTTATTAAGGATATGCCTAACAATATAATTGAAGCATCTCAAGATTTGGGATATTCAAAAACACAAACTGTTTTTAAAGTTATATTGCCTTACTGCATGAAGGCTATATTGAGTGGTTTTGGAGTTGTTCTTATGATGTCAGCAACATCAATAATTCTTTCAGATAAATTGTTACCAAATGGTAGTCAAAAGCAACTTATAGGAAATCTTATAAATCAGTTTGCCAATACAGCAAACCCTTACGACTTAGCAAATGCCTCTACATTAGTTATTATCACTTTAGCTGTTTTATTAACAATATATGGAATTATTTATGGAATACCTTATTTAATTTCAAAAAGAAAGCAAGGAGGAATATATGAATAAATTTAAAAAATTTTTGAAGTATTCTTATGTATTAATAATTCTTGCTTGTTTATATATTCCAATCATTTTTGGAACAATATATAGTTTTAATGCGCCTTCAGATAAAGGAATATTTTCTGTAACCTCATGAAATAGAACTTCTTATGAAGCGTATGTGGAACTTTTTTCTAAATCACATTGACTTGCATTTGTTAATTCTTTTTTACTAGGAATTTCAACATCTATTTTTGTTATATTACTTTCATTGATGACGGTTTTTGCACTTTGAAGGCATAAAAACAAAACAGTTAGAGCATATGTGCAATCAACATCAAATATTCCTTTAATTAATCCAGATGTTATTACTGGTTTAACGCTAGCAATTGTTTTAAATCTTATATTTTTTACCACACTAAAAGCAACCAACGAAGGATTTTTTAGGGCAATAATTTCACATACAGTAATGACTCTTCCTTATGGAATATTAATTATGTTGCCAAAAAGTGATAAATTTTCAAAAAATATCTTTGAAGCTAGCCAAGATCTAGGTTATTCAAAATTTAAAACATGATTTAAAACTTATTTTGTTTATATGCTAGGTTCAATAGGATTTACATTTGTAATCACAATGACACTTTCTTTTGATGACTTCATAATTACTAGAATTGTTTCAAATACAGAAACTTTGGGTACTCAATTATATGAAGGTCAATTTCAAGCATGATCGTTAGCTATTGGTGGAATATCATTACTAGTAGTTTTAATGGCAAATACAATATATGTAGCCATTAAATCATCGCAATTTTCACATAAAAAAATATCATCAATCAATATTTCTTCAATAAAAAAATAATATAGGAGGTAATTTTATAAATTGAGATCACGAATTTTAAAAGCTATTAGTTTAATATTTTTACCCTTGATTTTTATTGCTTTATTCCTTGCTTTTTTGGTTATAAAATCAACTAATCAATATAGACCTAGCATCTATAACTATGAATCTTATTTAGCCCCAACCATTATTAATAAATTAAAATCAAGATACAATTTCAAAGAATTCAAAGAAATTAGTGAGTTTACTCAAGCATTATTACAAGATAAGGCTATTGCTGGAATTGGAAGCGACTTTCAAGCTGCTCAACTTATTTTAGATAGAAAGATTCAAAAAATAGATTTTAGTCTAGTTTATGGTGAAAAATACAAAGATTGAAAAGAAAGACAATTTCTATATACCCCAAATGTTAGAGAGCATATTTTAAGATTTGACGCTCTAATTTTAAATATTTTAGAATCTGATAAATCTAATAAATATAGAAACTTTGAAATAAAGAAAAATAAAGAAGGTAAACCTATAGCATTTAGAGACAAATTAAACAATAACGGCAAGGAAGTTTGGGATCATTTTTATGATTATATCATTCCTTATTTTACCCAAGATAAAGGTATAGCTTACAATATAAATCCAAAGACTAGACCAAATTTAAATATAAACAACGCTATAGAAAAACTAGATAAAAGCAACATGATTTTTTCTTGGAATGAAATCTTGCAAATTCTAAGTAAAAGTAATTACACACATTTTGGCTGAACTCATGCTTATTTAGATAACATGATGATAGGGGCGTTTAACTCCGGAGAAAAATGAGAAGAAGTTTTTACTAAAAAAATCGGTGACAACAAAATTTTTGATTTTAATGAATCAAACTATAAATTAGCTATTGATTCATTTATAAATTTTGTTAAAAATTCTACCGGAAATGATATAAAAAACAATAAGAAGCATTACTTTAGTGGCGATGGTCTTGAATTATTAAATCATCTAATTGAACCAAAAACTTCTAGATCGGATGCCTCAATAATGTACAATGGTGATGCATTGGATGCATTCTATTCTGAAGATAATTTTCAATCGGTTGAAAGTGGTTTAATAAGGTTTATTAGACCAAAAAACACATATATGCTAATGGATAATTGAATAATTTCAAAAAGCCTTTCAAAAAAAGAAACAACAACTTTTTTAAAAGCCATAAGAGATCATGTTATAAATATTGATAAACACAAAAATAATATTCAAAATATTGAAAAAATATTTTTTAATCAATTTAAAAACAACATGACAAAACAATTAAAACAATCTGCTGAAGCTTCTTTTTTTGAAACCATACATAATATTGACAAAACTTTAAAAAATAAGTTAATTCAAGAATTTCTAAAAAACAAAGAATTTTTCAATATAAACGAAAAATCAACTAGCTTCGAAGAATGAAATAGCAAAAAGTTTAGAGAATTTTTAAAAAATCCTACTATTCAAGAAAATTATGAGTGATTTTTAATTTGAAGAAACAATTATCAAGACCATAATGAAGCTTTCTTATTTGATGATCTCATGTCTGATGCTTTTGCAAATAATGAAATAGGAAGTATCTCTAATTTTGATTATGTATCATATACTCCAACTGAAAAAGTAATTTATGATTTTATTAAAAAATGATATTTTGGCTCAAATAAAGTGGCAATATCAATATTCGATCAACCCGAATCAAATAACAATTACCAAGTTTTCTCATATCCAATTATTGACAATAATTTAAGAACAAAAATAATTACTTATTATTATGAAAAAACCAAGTCCTAAAATTAAAAGATCTATTCATAAATGTTTAATAAGATTTACAAAATTTAAATAATATATAATTTTTAGTATAAATTAAAAATTGAAAGGAAAAAAACGTCATGAACATAAAGCAATTATATAAGCAAATAAGCCAGTTACAAGAAAATGTTGAACTTACAATTGAAGGCTGAATTATTTCAAACCGTGGAAACGATAAAATTCGTTTTATTACAATTAATGATGGTAGTACAATTGCTAACATGCAATTAGTTGTTAAGGGGGATCTAGCATCAAAGAAAGAAATCTCTGAAATTGCCATGGGCTCAGCAGTTAGTGTAATAGGTAAATTACACTTAACGCCAGAAGCAATGCAACCATTTGAACTAAGTGTCTCAAAATTTTTATACATAAACAATCCACAAGAAGATTTTCCAATCCAAAAGAAAGAAACATCTCTTGATTTTTTAAGAGAAATTCCTCAATTAAGACATAGAACAAGTTTATTTAGAGCAATTATGTTAATCAGAAACTCTCTTATGTTTGAAATTCATAAATATTTCCAATCTAAAGGCTTTTTGAATCTTTCATCTCCAATAATCACATCAAACGATGGTGAAGGAGCGGGAGAAACTTTACTTGTTGACGATGAAAGCAAAGATTATTTTTTCAAACAAAAAGCATTTTTGGGGGTAACTGGACAATTGCACGCCGAATCATATGCTGAAGGATTTAAAAAAGTTTATACATTCGGACCAACATTTAGAGCTGAAAATTCTCACACTTCGCGTCATCTAGCAGAATTTTGAATGATGGAACCAGAAGTAGCCTTTTGTGATTTGAAAGGTATCATCGAAATTACAGATGATATGTTAAAGAATGTTATTGCAAATACATTAAAAAACTATCCCGAAGAAATGAAATATTTGGATTCATTGCAAAACAATAAATTAATTGAATCATTAAACAACTTTATCAATAAGAAATTAACAATAATTGAATATAAAGATGTTGTTGAAAATCTTAAGAACTATGTAGATAAATTTGAAGAAAAAGATATCTTTTTTGGAATGGACTTAGCTTCAGAACACGAAAAATTCCTTGCTGAAAACATCATAAAAGGCCCTGTTGCAGTTATTAATTATCCAAAAGACATAAAGGCTTTTTATATGTATCAAAATGATGATGGCAAAACAGTCGCTGCCTTTGACTTACTAGTACCAGGAATTGGAGAATTAGTAGGGGGAAGTCAACGTGAAAATAGATACCAAAAGCTTGTTAATAGAATTAACGAACTAAAAATCACTCAAGAAACATTACAATGATACTTAGACTTAAGAAGATTTGGTTACGCCCCAAGTAGTGGTTTTGGTTTAGGCTTTGAAAGGCTTGTAATGTATGTTACTGGCACAAGCAACATAAGAGACGTTATTCCTTTTCCTCGTGTTGCTGGCTCAATTAAAATGTAGGTTTCTATGCAAGAAAATAATTTAACAATATTAATTCCTATATATCATCCAACTCTAACAATTAATGAGATATTAAAAAATTTGTATAAACAAAAACAACAAAATTTTAATTTAGTAATCGCTATAGATAAGCCTTCTGAATCAGAACTTTATGAATTAGAAAAAGCCAAAACCTTTTTCTCAAATAGACTTCAAATAATAATCAATACAAATCACCAACACATTGATTCAATAATTAGTAGTTGCTTAAACATTACAAAAACAAAATACACATTTATAATGTATTCATATATTGAAATTGAGTATGATTTTATTTCAAAAATAAATAAACTTTTATTAGAATTTAAAGATGTTGATATTATAAGTTTTGACGCATATACAAAGGGTATATCTTGAATGAATTTCAACAAATATCCTAAACAAAATAAAGAAATAAATATTCATATTGAAGAAGAAAATGTTGCTAAAATAATTCCTTTTATGTACAATTTTATTGCAAAAACTTCTTTACTTATTGGTGCAACAAATCAACACAATAATAAACACCTTAGCGAGCAATATTCACCCAATATACTTTTTAAGACTTTAATTAATGCAAACAAAATTCTTTTAACAAAAGATATTCAAGTAATTGATTGAAATTATGATGTCCTATTGTGAAGCGTTAAATCTTTGTTGGAATCTTGAAAAGACATAGAGTCTGAGTATTCTATGCATGAATTTACAACTCCTTTTTCAGAAAGTTATTATTATCTTGCAAAATTTTTAAATATAGCATATTGTTTCGCAGGTTTTTTAGGCCAATGTCAATTTAAATCAAACACAAAAAACTATCTAACCTTAAAAAACCTTAAAAAACATTTAGAATCATCGATAAGTGAAAACATTGAAGAATGAAAAAATAGCAAAATTATCAATGATTTTTTAGAAAAAAACCGTATTCAACAACTTTTTGAATTAATACCAAATAGTAAAAAATGATCACTTATTTTTAAAAAAATAATATGATAAAAATAAATATTAAAGCTAATTTTTGAATAAGACTTGCTGCTACTTTGATAGATTTCTTGGTATTCATTGGTTTTTTAATCGGTTCTTCGTTTGCAATATTTAACTACAAGGAAGCAAAATTTTATAATTATTGATCTTATTATCTTTGAATAATAATGATAATTTTAGTCCAAAATATAATTTGAATAATCATTCCAATTTTTACCAAAGGTTATACTTTGGGTTTGTTTATTTGCAGACTAAAAATAATCAAACAGCAAAACAATAAATCAAAATTTAGCAAGGTTATTTTTGATAGGCAAAGGTTATTTAGTTTTATATGAATAATAATTTTTGCTTTATTTATGATTTTTATATCTCCCCAAACTTTTGAACAAGCAGCAAATCTTTCTCAAACAAAACCTCTAGATCAACTTCAAAAGGCTTTTCTTTCAATTCCTAGTGTGCTTTCGGCATTAGCCACATTTTTAGAATTCTTATTTATTTTAAGCAATGCCAAGGGAGATAGAATTGGTTTAAATGATAAATTTTCCGGATCCTATACAGTTTGAGCTAAAAAATATGAAGATATTGAAGAAAACAATATCGATAATTTGATTATAAAACCCATTGTTAGAGAATTACCAAAAATTGATTACAAATAGCAAAAAACAAATATTTAAATTTTGGTTAATTATAATTGAAATATGCACACCACAGAGGGGGTGTTGCTTTTTTATATTGCACTTTTGTGATATAATTTTATAATAAAATTTGCACATAAAAGGGGAATAAAATGATTGATTTAAAAGATTTAAATGAACAGCAAAAAAGTGCCGTTATATATAACGAAGGACCTTTAAGAATTATTGCTGGCGCCGGTTCAGGAAAAACTAGAGTTTTAACATATAAGATTGCTTATTTAATCCAAAAATTAGGTGTTCTACCTAATCGTATTTTGGCGCTAACTTTTTCAAATAAGGCTGCAAACGAAATGAAACAAAGGGTATTTAATCTACTTGCAGAAGATAACCCTAGCAATCTTTCTCCTTATATTTCAACATTCCATGCAATGTGTGCAAAAATATTGAGAAAAGAAATATATAACTTTGGATATGAAAACGATTTTCAAATTCTTGATGAATTAGACCAAAAAGAAATATTAAAAATTGTTTATTCAGAACTTGATATTTCACCAACAGAATTTACATTCTCTTCAATAATTTCATTTATTCAAAACAAAAAGAATTCAATCGAATATTTAAACAACTCAATTAACGATAATAACGAACATTTTAATGAAGACTCAGATTCACAAGAAACACAAGATGAAAAAATCAAGGCAATAAAAGAAAAAATTTACGAACGCTACCAATTACACCTCGAAAGATCTCACTCCCTTGATTTTGATGATTTGTTGGTATTTGTATACAAATTATTTTATGAACCAAAATTCAGTTTTGTAGCTGAAAGATGAAAAAAGAGATTTGATTATATTTTAATAGATGAATTTCAAGATACCAGCATTTTGCAATATAGAATAATGCAAAAACTTGCAAATGAACATTTAACCATTGTTGGTGATCCAGATCAAACAATTTATTCTTGAAGAAATGCTGACATAAACATTATTATGAATTTTGATAAGGATTACCCAAATTCAGTTACAGTAAAACTTGAAGAAAATTACAGATCAACCAAAACTATCTTAAGAGCAGCAAACAACTTAATTTCGCATAATAAACTTAGGTTAGAAAAGAAATTATTCACTGAAAACGAAGAAGGCGAAGATATCGAGTTTTATTGTGGTTTTAATGACGAAGCAGAAGCTAGATGAATAGCAACAAAAATTTCTGAATTAAAAAGAAATCGAGTTCAATTAAAAAATATTGCTATTTTATATAGAACCAATAGTTGTTCAAGAGCAATTGAAGAAGCCTTAATCAAAGAAAATACAATATATAAATTATTCGGCTCTATTAAATTTTATCAAAGAGAAGAAGTAAAAGATGCCCTAGCATATTTAAGAGTTATTCACGATGGAAGCGAAATTTCGTTATTAAGAATAATTAATAAACCTTCTCGTAAAATAGGGAATGTTACAATCGAAAAACTTCTTTCTGCTTCTAAAAAATACAACTTAGGACTTTTTGAATATCTAGAAACTCACTTTAACCAAATTCAAAAAGAATTACATATTTCCGGCGATACATTAAAGAATTTAGCAAACTTTATCAATGAAATCAGATGAGCTAGAAGAGCAATTCAAACAAATGCAATTCACTTAACCATAAAAGAATTAATCGTTAATAAAATAAAATACTTTGACGAAATAAAAAACTCAGAAGAAGAATATGAAAATAAAATCGACAACTTCAATAGTTTAATTGAAGCCATTGCTGAATGAGAAAAGAAAAACCCACAAGGTTCTATTGATGAATATTTACAAGAAATAACTTTAATAACAGATAGAGATGTTGAAGATGATGCCGCAAGTTTCGTTTCACTAATGACTGTTCACAACTCAAAAGGGCTTGAATTTGATTATGTATTTGTTGCTTGCTTAGCAGAAAATATCTTTCCATTAAAGAGGGCCATATCGATTTCTCCACAAGACGATTTTACTTTTTTAAATTCAAAAAATTTCAAAGAAAACATAGAAGGATTAGAGGAAGAAAGGCGTTTGGCTTATGTAGCAATGACTCGTGCTAAAAAAAGATTAATGCTTTCTTTCTCAGTTGGTAGAAATGGTATAAATAGAAAAAGTAGATTCTTGTCTGAAGCAGGAATAAACGAAATTAGAACAATAAAGATTGCCAACAACTTCTCAATTGCAGCAGAGCAAGAAACAAGAAATAACGATTTAATAGTCGGTGATTATATTATTCACAACACATATGGAAAAGGGTTGATTATAGATATGGTTGACAACGTTATTGAAGTTAAATTTGAAAATGATAAAAAGATTCGCAAATTATCAAAAACCCACCATTCAATTAAAAAATACGAAGATGATGAAAGCCTAAATAGTTAAAATGTATAAATACATAGCAATATGAATCTTTCTAATAATTTTATTCTTGGCCGTATCAACCGTATTAACAATATATTACGTTATTATTAGACGAAAGAAGATAAATGCACAAACAGGATTTTTTGTATTTAAAATAGACAGTACAAAAAACAGAATAAAAATCGAAGGCGATATAAATTCAATTAACAAAATACCTAAATATTTATGAAAGATTTTAAGTATAGATGGTCAATGGGCAAAACTAAATAACTTCTTAAATATTTTTGATTCAGGTGATAAGCGCCTATTACTAGATAATATAAAGAAAAAAAACTTCTTTAAACTAAAAACAAAGATAGCAGAAGAATCATGATCTAACTATTTTTCAAAAGTAGATATTGAGTTTAACAATGTTGTAAATGATGAAATTATTGGAACATTTACATGAAGAGACATTGACATTAAAATGGATAATGTTTTCAATAAAGAAATTCATGATTTGTCTTATATTCCAACCATTGAAAATTATGAAGGCTTGTTTTTCTTTTTAAACAAGAAACACATTACTGATATAAATGCTTTTTTAGATCTGTTTAAAGATTTAGGAAAACAACACAATATTAATGGCTTAGAAGTTGTGTATGAATGAAATGTTCTTTGCATTTTGTTTAATCATGATAAATTAGGTATTGAGAAAGCAAAATTTCTTGCACAAAAATTCATTGATATTGCAAAACTATACAACAAACTTTATTTTAAACTTTGTTGATTTGATTCGTATATAATGAATAAAAAAACATTGGATAACGTATTTACATTCTTTGATTATTTAAAATTAGAAACATTTGAAAAAAATGCATATAACTTTCAAAAAATCCCAAGCAATATTTGAACCGAGCAAAACTTTCAAAAATTTTGTTTAACATACGAAAACATTATTTATAATCTAAGAAATAATTTTTCTATTTCGCTAAAAAATATTTCGCTAAAAAATGCAATTGGCGATAAAACCGAACTTAGAAGGTTTGGACTTCCTAAGATTTTTAATTCATTAAAATTAAACAAAGATTATGACTATTACCTAGAATTTTTAGACATAACTAGATTATTTTATAATGTCTTTTATGAAAAAATTGGTGAACTACATCCTAAAAATGCAATATTAAAAATTAGTGATTCAATATTTGAAACATTAGATATAAACGTTATTAAAAAAATCGGGGTTGAATTTCCAACATTTATTCAATCAATAAAATTATTATCAGCAGAAAGAATAAAAGACATATCCCAAAAAATTGTTAAACTACAAGGAACAAAACTCAATTTTTGTCTCGAAATTAAGGATATTGATTCAACAATAATTTCTCTGGTATCCCCTTGAGTTAAATTCATTTGAATAGATGAAGAACTAACCTCTAGATTAACCGAACCTAACATTGTTTTATATCTAAATACCTTATTAGATAATGTAAAAAATCTAGGTATTAAAGTTATTTTTGAAAAATTAGACTACAAAACATATAGGAAATTATTTTATAAACACACATCGATTATTTTATATACGACCAATTAGCGTTTTAATATAAAATTATTAAAAAATCAAAACAAAAAAGGACAAAACATGAACAAAAAACTTATTCCAATAGATTTCTTTGATGCAGTAAATAAAAAGTGGGTAGATAGCCATAAAATCCCTGATTCTAAAACCTCTATAGGTTGCTTTGCGCAATTAGATAAAAATATTACTACATTAACTAAAAAAATATTAAATACATGAAGTGAGGATGCCTCAACAATTCCAAACGACCCTATTATCAATGAACTAGTAAAATTTTACAGCTTAACAAAGAACTGAAAATTACGTTATCAAGCACAATTAAAACCAATTAAAAAATTGCTTTCAAAAATTAACTCTTTAAATTCTTGAAAAGATGTTGAAAACAATTTTTTGGAATTGAAATTATCTAGTTTAAACACCCCTTTAATTTTTTCAGTTTCAACAGATTTTAAAAACAGCGATGTTCAAACTCTTTATTTAGATGTAAACTCTACAATTCTACCAGACAAAAGTTTTTATACCGATCCTGCCAAAAAAGAAAAATTTATTAATGTTTGAAAAAATATGGTTTTAAAACTTTTAAATAAGATCGACAAAAACGAATCTAAAAATAATTTAATCATTGAAAATGCTTTAAAATGAGATGAATTAACTTCTTCATTTTTAAGAGATTCAGAATTCTATTTTGATATGGTTAAAATTTGCAACACTATTTCAACAAAAGAATTAGCCCAAAACATTAAAACAATAAACATAGAAAATATATTAAGACAATTAATTAAATGTTTCCCCGATGAAATAAATGCTATTGATCCAAATTCTATAAACATTGCAAAGTCTTTAACCAATGAAGAAAATTTTGAATATTATAAAGCAAGTTTAATTATTGATGCAATATTAACCTATGCACCTTATTTTGATTATTCAACAATTCAAATTGCTTCTGAATTTAATTTAGCAATAAACGGAAACAAAAAAATTGAATCAAAAGAAAAATATCAAATTAAACAAGCAAAACGTTTTTTTGATATTCCTTTTGGAACTTACTATGGTAAAACCTATTTTGGATTAAAAAACAAGCAAACAGTTGAACACATGATAAAAAGTATGATAGATGTTTATAAAGAACAACTAACAAAAAACTCTTGGTTAAGTAAGCAAACAAAAGAAAAAGCAATAATAAAACTTAACTCAATAGGTGTTTACGTTGGCTTTCCTGAAAAAATTCAAGATCACTATAAATATTTAATAGTTGAAGAATTTGATGGATACAATGATTTATTTGAAAATGTTTTAAAATTTAACAGAATTTTAGCAGAACACATCTTATCCGAATATGGAAAAACAGAAGACAAAAACCTTTGACATATGTCGCCTGCTATGGTTAACGCCTATTATAATCCTTCAAAAAATGTAATAGTATTCCCTGCTGCAATCTTGCAAAAACCTTTCTTTAGTGCAGATCAATCCTCTTCATCAAATTTCGGCGGAATTGGTGCTGTTATTGCCCATGAAATATCACACGGATTTGACAATAACGGAGCAAATTTCGACGAAAAAGGAAATATGATTAATTGATGAACAGAAGAAGATAAAAAAGCTTTTGAAATCAAGACAAAAGAAATGATTAATTTATTTGAAGGTAGAGAAACATCTGTTGGTAAATGTAATGGAACATTAACCGTTTCAGAAAACATAGCAGATGCTGGTGGATTATCGTGTGCTTTAGCAGCAGCAAAATTAGAAAAAGATTATAACCCTAAAGATTTTTATATAAATTTTGCAACAATTTGAAGAACAAAATACCGTCCAGAATTACAATCATTATTATTAACTATTGATTGTCACGCTCCTGCCAAATTAAGAACAAATGTTCAAATACAAAACTCTGATGATTTTTATACAACTTTCAACGTCAAAAAAGGCGACCCAATGTATTTACCACCTGAAAAAAGAGTAAAAATTTGATAATTCTAAACACAAAAAGCAACCTCGTGGTTGCTTTTTAATTAACACAAATCTTCTTTCTTTATTCCTAAAAGAACATACAGCATCACTCGTTTAATTCTGCTATTTGTATATCTTTTCGAAGTACATAAATCAACAAATTCATTATAATTTTTTGCTATTTCAATATTCTTTTTAAAAAGATTTTCCATTCCTTCGGATATTAATGCTATATTTGCTAGTTCTTCTTTAGAAGAATTTTGTACCTTTTGTTTAAACTCATTATATTTATCTTCAATTTTTGAAAAATTTTTAGGAATGTTTATCGGTGTGAACATTGAAACATCTTCGCCTTCTTCTAACATTTTTCGTATCATTGTTGCCGAAGCATAATTATTATTTGCTATTAACGAATTGTGTGGAGCTGTTCTTTTTATGCAAAACGGTTTTACTGATAAATTATTAAAAACTATATATTTGGTATATTCAAACCCTAAAATATCTTGTGGTATATTTTCAACACCTATTAATTTTTCTAGCGACAAGAAACAACTCAAAACATATGATTTTCCTGTTTTTAAATTCATTCTTACATCTTTGTTATATTGATCAAAATTTTTCTTTATTACGTTTGCTGCGTTTAAATATTTTTTTATATCATCAGTGTCAGAAACCCCAAAAACTAAATAATCAATACCTTCTTTTATTAACAAATCTATTGATTTTTTAGCAAAAATATGGGCTGCTTGTGTAGATGCAAAAAAATCTAATTCAACAACTTTTGTTGCTCCATATTCAAGCGCAATGTTTTTTCTAACTTCAAAAGGTAAACATGCTATTTCGCCCCGTTGAACATAATCGCAAGATAATGCAACAATTATTTCAGGATCTTTGAAGATTTCTTTAATTTTATTAATTAAATAAATATGCCCATTGTGAAATGGGTTAAATTCAGCTATTATTCCTATTTTCATTTATTTTTTCTTTAATTCATCAAGGCTAATATCGCAAAAATCTTCAATAGAGCTATCTTCTCAATAATTTAATTCCACAGTTTCTTGGATCTCAATGTTTTTTTGATCGCCTTCACAATCTTTGTTTGAATCAAAATCTTCGAACATCACAATATCGCTTTGTTCTTTTTCTTTTTTTATTTGTTTTTTCTTGCTTGTTTTCTTTTTATTAAACATATTAAAATTATATCATTGCAAAAAATTACTTTTATTTTTTTAATTGTGTGATTAAAATGTTAATAAATTTTTTCGAAATTTCTTTTTAATTTTAGTTGTTTTTTAAATTGTTAACATTATAAACATAGCTTATTATATTTATTAATTTAACAAAGCAAACCAATAACACCTAACATTAAACAAAAACATTTTTATATATTATTTAATATATAATTAAAATATGAAAAATAATATTCAAGATACAATAGTTGCTATTTCTTCAGGCAATGTAAATCAAGCAATATCTATTATCAGATTATCTGGTGAAGATTCAATTGAGATTGTTAAAAAAGTTTTTAGTGGTAAAGTTGGAAAAGATAAGACAATTACTTACGGAAATATCATTGATAATATAACAGGAAAAATCGTTGATGAGGTATTAGTTAATTGGTTTATTGGTAATAAAAATTATACTGGAGAAAATACCGTTGAAATAAATGCTCATGGTGGAATTGTTGTAACAAACAAAATACTTAATCTATTAATTGCTAATGGTGCAAGGTTGGCAAACAGAGGAGAATTTACAAGGCGAGCATTTTTAAACGGAAAAATTTCGTTAGATAAAGCCGAAGCGATAAATAGCTTAATTCATGCAAAAACAAATAAACAAGCAGAAATTGCTATTTCTCAATTTAATTCTAAGGACAATTTAATTATTGAAAATTTAATTAACGAACTACTTACTATAATTTCATTAATCGAAATTAATATTGATTATTCTGATTATAACGATATAAAAGAAATGGATGCTAACACCTTAACAAGTCAATTATCAAAATTTAATAGAAAATTAGATTCTATAATTAATAAATCAGAAAATGCTTGCGATATTTATAAAGGAATAAATGTTGCGATAGTGGGAAGACCCAACACTGGTAAATCATCACTTTTAAATTCTTTAATTGGTTCACAAAAAGCAATAGTAACAAATATACCCGGAACAACAAGAGACGTGGTAGAAGGTGAATTTCAGATTAATGGTTTTTTATTTAATTTGATTGATACAGCGGGTATAAGACAAACAAAAGATCTTGTTGAATCAATAGGTATTGAAAGATCTAAAAACGCAATAAAAGAAGCCAAAATTGTAATTCATATGCACGAACCATTTATTAAAGAAAATGAAGAAGATAAAATAATTAAAGAACTCTCAAAAGGTAAGGTATATATACCAGTAATGAATAAGCAAGATCTTTTAAATAAAGATCAATATCCTAAGAATATGGTTTTAATCTCAAGCAAAGAAAATAGTCTATGAGAATTAAAAAATGCGTTAGTAAAAAATTACTTGAATATTGATTTAGATGACCCTCAAATAATTTTTAATAATAGAAAGCTTTCGTTATTGAAGCAAGCGAAGATTTCTATCAATACTGCACTAGAATCACTAAGTCAAGGTTTAGGCCCTGAGGTGGTTATTTTAGATATAAACGAAGCTTGAGGGTCATTGAGCGAAATACTAAATAAAAAGCACGATAATGAAGCCCTACTAGATAATATATTTAGCAAATTTTGTTTAGGAAAATAATATGTATAAAATAAACGACAAAATAGTTGTGAAAGCAACAGAACTAAGTTATGAAGGCTATGGGGTTGTAAGAAGTGATAAACAAACACTTTTTGTTGAAAATCTCTTACCTGATGAAGAAGCAGAAGTAGCCATTTATAAATCAAATTCAAAAATTGCTTATGGACGTACAGTTAATTTGATAAAAAAATCATGTAAAAGAGTTGAAGTTGTTGAGAAAGAATTGTTTGAAAGTGGTGCAGCGCCATTGATGGTTTTAAGTTACGAAGATCAATTAAAATTCAAACAAGAAATTGTTAATAATTTAATAAAAAGAAATATTGGATTTCAAAACATCGAACAAATTCAACCTAGTCCAAAAATATTAAATTATAGAAATAAAATAACCTTACATGTGAATTATAAAAACAATAATATTTATATTGGTTTCTATAAAAAAAACAGCAACAAACTAGTTATTCAAAATTCGCTTCCTCTTTGTCATGATGAACTTGATAAATATTACAAAGAACTTATTAGTGCCCTAAAACAACAAATTTTATCAAAAGATAATTGAATAAAAGAACTTAAACTTAGACAAATTACATTGAGGCATAGTAATTGAAGCCATAATATTGAAGTTATTTTTATTATTGAAAATCCAAATAAAAAAGGTTTTGATTTATTGAAAAATAATTTGATTTTCTTATTGAAACCCAATGTAATTTCAATACAATGCCAATATATTAAAGAATCTAAAGATATATTCACTTCTTTGTATAAATCAATGCCACAACAATTTAATATAAACAATAAAATATTCAATGTGAACAATGATTCTTTTTTTCAAGTAAATGAAGAACAAACTAGTTTAATATATAATCAAATTAAAAAATTCATAGATCAAGAAAATTCAACAAAAATAATTGATGTTTATTCAGGTGTTGGTAGCATTGGAATATCTGTTTGTGGCAATGACTCTCAACTTGAATTAATTGAAATAAATAAATCGGCGTGTGAGTGTGCAAAAATTAACATAAACCAAAATTTGATTAATCCAAAACTAGCGGATATAACAAATAGTTCAGCACAAGACTTTTTTAATAAAAAAGAAGCAGAATATTTTGAAAAATCGATAGTTATATTTGACCCACCAAGAACGGGTTTAACAAATAAAGAAATTGATATGCTAGAAAAATGCAATAGTGTAATTTATATGAGTTGCAATCCTCATACAATGATTAGGGATTTAAAATATTTTGAAGAAAAAGGGTATAGAATCGAAAGGCTAATACCTTATGATATGTTTCCTCAAACTTATCATATTGAGACGGTAGCACTTTTGTCCAAACTTGATGTCGATAAGCATATAAATATTGAAATTGAGCTGGATGAGCTTGATTTGACAAGTGCTGAAAGTAAGGCAACATATGCTCAAATCAAAGAATATGTTTGGAATAAATTTGAATTAAAAGTTCCGACATTATATATTGCACAGATAAAAAGGAAATGTGGAATAGAATTACGAGAACATTACAACAAGTCTAAAAAGGAGAAACAAATTATTCCACAGTGTACACCTGAAAAAGAAGAAGCTATCATGGATGCCTTGAGACACTTCAAAATGATTGAATAGAAAGGGAAGATATTTTATGAGATGGACAATAAAAATAATCTTTTTCCCAATCAGCTTCCTGCTTAGTATTCTCACTGCATTTCTGACATTCTTACTCGGAATAGGAACGGCACTGCTATATTTGCTGATGATGTTTTGCATATTTGGAGCAATAGCATCTTTCTTACAAAAAGAAGTTACCATCGGAATCGAAGCATTGATTATCGGATTTTTAGTTAGTCCATACGGCGTACCGATCGGAATTACCCGAAGAATTACAATCGGAGCTATCTGCTCTGCTCACTCATTTTTCAAGCATTGAAGAACTAAGCGAACATCAAGAGGATATTATCATTCATTCCGATTGTGATGATATGTATGACGTGGCACGCTACTACATTGAAGAAACGGGTGCTTTAGGCGAAGTACCAGCTAGTCTTCAAAACTATATTGATTATCAAGCCTATGGTCGGGATTTAGACCTTTCAGGAACGTTTATCTCAACCAATCATGGGATTTTTGAAATCGTCTATTAAATCTGTCGGTACATTACTACTGGCAGATTTTCTATTTTACGGGGTGGCTCAATCAGCTACCCCTATTTTTTATGAAAGGATTGATTACATGAAGAAAATACGAAGCTATACCAGTATCTGGTCTGTGGAAAAGGTACTGTATTCTATCAATGATTTTAGACTTCCGTTTCCCATAACCTTTACGCAAATGACATGGTTTGTCGTGTCACTCTTTGCAGTGATGATACTTGGCAACTTGCCCCCTCTTTCCATGATAGAGGGAGCATTTCTCAAATACTTTGGGATTCCTGTGGCTTTCACATGGTTTATGTCTACAAAAACTTTTGATGGTAAAAAGCCTTATGGATTTTTGAAGTCTGTCATTGCTTATGCACTGCGACCAAAGCTGACCTATGCAGGAAAAAAAGTAACGCTTGGCAGAAACCAGCCACAAGAAGCCATTACAGCAGTTAGGAGTGAATTTTATGGCATATCCAATTAAATACATTGAAAACAATCTCGTCTGGAATAAAGACGGGGAATGTTATGCTTACTATGAGCTTGTTCCTTACAATTACTCATTTCTAAGTCCAGAACAGAAAATACAAGTGCATGATTCTTTCAGACAGCTTATCGCACAAAATCGTGATGGCAAAATTCATGCTTTACAAATCAGTACAGAATCCAGCATACGTTCTGCACAAGAGCGTTCCAAAAATGAAGTCACTGGCAAGCTCAAAGCGGTTGCCTATGACAAAATCGACCAACAGACAGACGCTTTAATATCCATGATTGGCGAAAATCAAGTGAACTACCGTTTCTTTATCGGCTTTAAGTTGCTTCTCAACGATCAGGAGTTTTCTATGAAAAGTCTTACCGTTGAAGCAAAAAATGCTTTGTCTGATTTTGTCTATGATGTGAACCATAAGCTGATGGGCGATTTTGTTAGTATGAGTAATGATGAAATCCTGCGTTTTCAGAAGATGGAAAAGCTCTTAGAAAATAAAATCTCTCGTCGTTTCAAAATCCGCAGGTTAGATAAGGACGACTTCGGCTATCTGATTGAACACCTTTACGGACAGACAGGCACTGCCTATGAAGAGTATGAGTACCATCTATCAAAGAAAAAGCTGGATAATGAAACGCTGATTAAATACTATGACTTGATTAAGCCTACTCGCTGTTTGGTGGAAGAAAAACAGCGATATTTGAAAATCCAGCAGGAAGATGAAACCGTCTATGTAGCTTACTTTACCATTAACAGCATTGTCGGAGAACTGGACTTCCCGTCCTCTGAAATCTTCTACTACCAGCAACAGCAATTTACATTCCCGATTGATACGTCAATGAATGTGGAAATTGTAGCGAATCGTAAAGCCCTATCTACTGTCCGCAATAAAAAGAAAGAACTGAAAGACTTGGATAACCACGCTTGGCAAAGTGATAATGAAACCAGCTCCAATGTGGCGGAAGCTCTGGAAAGTGTGAATGAGTTGGAAACCAATTTAGACCAAAGCAAGGAATCTATGTACAAGCTGTCTTATGTGGTAAGGGTATCAGCAAATGATCTTGACGAACTCAAACGTCGTTGTAATGAAGTGAAAGATTTTTATGACGATTTAAGCGTAAAACTGGTACGACCATTTGGGGATATGCTCGGCTTACATGAAGAATTTTTACCTGCCAGCAAGCGTTATATGAATGATTATATTCAATACGTGACCTCTGATTTCCTCGCTGGTTTAGGTTTTGGTGCTACTCAAATGCTGGGGGAAAATGAGGGGATTTATGTTGGCTACAGCTTAGAAAAACAACTGAACATTATACAGGTTGCGGACTTGGTACTTCCCGACAAGGAAACTTCCTTTGAGGAATATACCACTATGGAGCTTTTATCCGTTGCTATGCTGATTGTCATTAGTACCTTTGCTTTAGACTTTATCCATACAGACCGAAGCATTTTCAAGATTGTAGATTTAGACGAAGCATGGAGCTTTTTACAGGTAGCACAAGGAAAAACACTATCTATGAAGCTGGTTCGGGCTGGTCGTGCTATGAACGCTGGGGTATATTTCGTGACCCAAAATACAGACGACCTCTTAGATGAAAAACTGAAAAATAACCTCGGCTTAAAATTTGCATTTCGTTCCACTGACCTTAACGAGATTAAAAAGACCTTAGCCTTTTTTGGTGTAGACCCAGAGGACGAAAACAATCAGAAGCGATTGCGTGATTTGGAAAACAGGCAATGCCTTATCAGTGATTTATATGGTCGTGTCGGTGTGATACAGTTCCACCCTGTATTTGAAGAACTGCTCCATGCCTTTGATACCAGACCACCTGTGCGAAAAGAGGTGTAAATGTGAAACCATCAATAGTAAACAGAATAAAATCAAACTGGACGCTGAAACGTCTAGGTAAAGTGGCAATGACAGTGGCTTTCACACTTGTGATTGCCATTTTTCTTTTAGCCATGCTGGGAACGGTGGTTCAAGCTGCGGGCTTGGTAGATGATACGGTCAATGTGGCAAATGAATACAGCCGATACCCACTTGAAAACTATCAACTGGATTTTTATGTGGATAATAGCTGGGGCTGGCTTCCGTGGAACTGGTCGGACGGGATTGGAAAACAGGTCATGTATGGACTATATGCCATTACCAATTTTATTTGGACAATCAGTTTGTATGTTTCCAATGCGACAGGTTACTTAGTACAGGAAGCCTATTCCTTAGACTTCATTTCCGCTACAGCAGATTCCATTGGTAAGAATATGCAGACCTTAGCTGGTGTGAGTGCAAACGGATTTTCAACAGAGGGTTTCTATGTTGGATTCCTCTTACTCTTGATTTTGGTTCTTGGGGTTTATGTTGCCTATACGGGACTGATAAAGAGAGAAACCACAAAGGCAATTCATGCCATTATGAATTTTGTGCTGGTGTTTATCCTATCGGCTTCCTTTATTGCCTACGCTCCCGACTACATTAAAAAAATCAATGACTTTTCATCAGACATCAGTAATGCCAGTTTATCACTTGGCACGAAGATTGTCATGCCCCATTCCGATAGTCAAGGCAAGGACAGCGTGGACTTAATCAGAGATAGCCTGTTTTCCATACAGGTTCAGCAACCGTGGCTACTGCTTCAATACAACAGTTCAGACATTGAAAGTATCGGTATTGACCGTGTGGAAAGCCTGCTCTCCACCAGCCCAGATTCCAACAATGGCGAAGACAGAGAAAAAATTGTTGCGGAAGAAATTGAAGACAGAAGCAATACCAATCTAACCATTACAAAGACCATTAACCGTTTAGGTACAGTCTTCTTCCTATTTGTCTTCAATATTGGGATTTCCATATTTGTATTCCTATTAACAGGAATCATGATTTTCTCGCAGGTACTTTTCATCATCTATGCTATGTTTCTGCCTGTGAGCTTTATTTTAAGCATGATTCCATCATTTGATGGTATGTCAAAACGAGCCATAACAAAGCTCTTTAATACCATTTTGACACGAGCTGGAATCACATTGATTATTACGACAGCATTTAGTATTTCAACCATGCTCTATACCTTATCGGCTGGTTATCCGTTCTTTTTGATTGCTTTTCTACAGATTGTGACCTTTGCAGGAATCTACTTCAAGCTGGGCGATTTAATGAGTATGTTTTCTCTACAGAGTAACGATTCTCAAAGTGTGGGAAGTCGTGTGATGAGAAAACCTCGTATGCTTATGCACGCTCACATGCACCGTCTACAGCGGAAACTTGGACGTTCCATGACTACTCTAGGGGCTGGGTCTGCCATTGTTACAGGTAAAAAAGGACAGTCGGGTTCGGGGAGTTCTGCAAGGACACAAGCAGATCACTCCCGACCAGACGGAAAGGAAAAATCAACACTTGGAAAACGTATCGGTCAAACCATCGGTACAGTAGCTGATACCAAAGACAGAATGGTAGACACTGCTAGTGGTTTGAAAGAACAGGTTAAAGATTTGCCGACCAATGCAAGATATGCAGTATATCAAGGAAAATCCAAAGTAAAAGAGAATGTCCGTGATTTAACCAGTAGTATTTCTCAAACCAAAGCGGACAGAGCCAGTGGACGCAAGGAACAGCAGGAACAAAGGCGAAAAACCATTGCGAAGCGTCGCTCTGAAATGGAACAGGTCAAACAGAAAAAACAGCCTGCTTCTTCTGTTCATGAAAGACCGACTATAAGACAAGAACAATATCATGATGAACAGACCTCAAAACAGTCTAATATTCAGACTTCATATAAGGAATCTCAACAAGCCAAACAAGAGCGTCCAGCAGTTAAGTCCGATTTTTCAAGTCCAAAAGTGGAACGCCAAGGCAATACCGTTCAAGAAAAAACCGTTCAAAAGCCAGCAACTTCAACCACTACAGCAGATAGAACTTCACAACGTCCAATCACAAAAGAACGTCCGTCTACTGTTCAAAGAGTACCACTACAAAATACAAGAAGTAGACCACCAATCAAAACCGCCACCATTAAGAAAGTCGGTAAGAAACCATGAAGTTGAAAACTTTAGTGATTGGTGGTTCTGGATTATTCTTGATGGTCTTCTCACTGCTTCTGTTTGTTGCCATTTTATTTTCAGATGAACAGGACAGCGGAATTTCCAATATTCATTATGGAGGTGTGAATGTTTCCGCAGAAGTGCTGGCTCATAAGCCTATGGTAGAAAAATATGCCAAAGAATATGGCGTTGAAGAATATGTCAACATACTTCTTGCGATTATACAGGTGGAATCGGGCGGTACTGCGGAAGATGTTATGCAGTCCTCGGAATCCCTCGGTCTTCCACCTAATTCATTGAGTACAGAAGAATCCATTAAGCAAGGTGTGAAGTATTTCAGTGAATTATTAGCCAGTAGCGAAAGGCTCAGTGTAGATTTAGAATCGGTTATCCAGTCCTACAATTATGGTGGTGGTTTCTTAGGGTATGTGGCTAATCGTGGAAATAAATATACCTTTGAACTGGCTCAAAGTTTCTCAAAAGAGTATTCAGGTGGCGAAAAAGTGTCTTACCCCAATCCCATAGCCATACCTATCAATGGGGGCTGGCGATACAACTATGGCAATATGTTTTATGTGCAACTGGTAACGCAGTATCTTGTCACAACAGAGTTTGATGATGATACGGTACAAGCCATCATGGACGAAGCACTGAAATATGAGGGCTGGCGATACGTTTACGGTGGAGCTTCCCCGACTACTTCTTTTGATTGTAGCGGACTGACACAATGGACGTATGGAAAAGCTGGAATTAACTTACCACGAACCGCACAACAGCAATATGATGTGACCCAGCATATCCCACTATCGGAAGCACAAGCTGGCGATTTGGTTTTCTTTCATTCTACCTATAACGCTGGCTCTTATATTACTCATGTTGGGATATACCTTGGCAATAACCGTATGTTTCATGCAGGCGACCCAATCGGTTATGCCGACTTAACAAGCCCCTACTGGCAACAGCATTTAGTGGGAGCAGGACGAATCAAACAATGAGAAAGGAAGATTTAATGATGAAATTTAGAAAAAATCAGAATAAAGAAAAACAGATACCAAAGGAAAAGAAACCTCGTGTCTACTATAAGGTCAATCCTCATAAAAAGGTTGTGATTGCCTTGTGGGTACTTTTAGGGCTTAGTTTCAGCTTTGCGATATTCAAGCACTTTACAGCTATAGATACTCATACTATTCACGAAACAACTATCATAGAAAAGGAATACGTTGATACTCATCATGTAGAAAATTTTGTAGAGAACTTTGCGAAAGTCTACTATTCATGGGAGCAATCCGATAAGTCCATTGATAATCGAATGGAAAGTCTAAAAGGCTATCTGACAGATGAACTTCAAGCTCTCAATGTTGATACAGTACGCAAAGATATTCCTGTATCGTCTTCTGTAAGAGGATTTCAGATATGGACGGTAGAGCCAACTGGCGACAATGAGTTTAATGTAACCTACAGTGTAGACCAGCTCATTACAGAGGGAGAAAATACAAAGACCGTCCACTCTGCTTATATAGTGAGTGTCTATGTAGATGGTTCTGGAAATATGGTACTGGTTAAGAATCCGACCATTACCAACATACCTAAGAAATCAAGTTATAAACCAAAAGCCATTGAAAGTGAGGGGACGGTTGATTCCATTACAACCAATGAAATCAATGAGTTTTTAACGACGTTCTTCAAGCTCTATCCTACAGCGACAGCCAGTGAACTTTCCTACTATGTGAATGACGGGATATTAAAACCAATCGGAAAAGAGTACATCTTTCAAGAACTGGTAAATCCTATTCACAATCGTAAGGATAATCAAGTCACGGTATCGCTGACAGTGGAGTATATCGACCAGCAGACCAAAGCAACGCAGGTATCTCAATTTGATTTGGTACTTGAAAAGAACAGGAGTAATTGGAAGATTATAGAATAACAAATATTGGTACATTATTACAGCTATTTTGTAATCACGTACTCTCTTTGATAAAAAATTGGAGATTCCTTTACAAATATGCTCTTATGTGCTATTATTTAAGTATCTATTTAAAAGGAGTTAATAAATATGCGGCAAGGTATTCTTAAATAAACTGTCAATTTGATAGTGGGAACAAATAATTGGATGTCCTTTTTTAGGAGGGCTTAGTTTTTTGTACCCAGTTTAAGAATACCTTTATCATGTGATTCTAAAGTATCCAGAGAATATCTGTATGCTTTGTATACCTATGGTTATGCATAAAAATCCCAGTGATAAAAGTATTTATCACTGGGATTTTTATGCCCTTTTGGGTTTTTGAATGGAGGAAAATCACATGAAAATTATTAATATTGGAGTTTTAGCTCATGTTGATGCAGGAAAAACTACCTTAACAGAAAGCTTATTATATAACAGTGGAGCGATTACAGAATTAGGAAGCGTGGACAAAGGTACAACGAGGACGGATAATACGCTTTTAGAACGTCAGAGAGGAATTACAATTCAGACAGGAATAACCTCTTTTCAGTGGGAAAATACGAAGGTGAACATCATAGACACGCCAGGACATATGGATTTCTTAGCAGAAGTATATCGTTCATTATCAGTTTTAGATGGGGCAATTCTACTGATTTCTGCAAAAGATGGCGTACAAGCACAAACTCGTATATTATTTCATGCACTTAGGAAAATGGGGATTCCCACAATCTTTTTTATCAATAAGATTGACCAAAATGGAATTGATTTATCAACGGTTTATCAGGATATTAAAGAGAAACTTTCTGCCGAAATTGTAATCAAACAGAAGGTAGAACTGTATCCTAATATGTGTGTGACGAACTTTACCGAATCTGAACAATGGGATACGGTAATAGAGGGAAACGATGACCTTTTAGAGAAATATATGTCCGGTAAATCATTAGAAGCATTGGAACTCGAACAAGAGGAGAGCATAAGATTTCAGAATTGTTCCCTGTTCCCTGTTTATCACGGAAGTGCAAAAAACAATATAGGGATTGATAACCTTATAGAAGTGATTACGAATAAATTTTATTCATCAACACATCGAGGTCAGTCTGAACTTTGCGGAAAAGTTTTCAAAATTGAGTATTCGGAAAAAAGACAGCGTCTTGCATATATACGTCTTTATAGTGGCGTACTGCATTTGCGAGATTCGGTTAGAATATCGGAAAAGGAAAAAATAAAAATTACAGAAATGTATACTTCAATAAATGGTGAATTATGTAAAATCGATAAGGCTTATTCTGGAGAAATTGTTATTTTGCAAAATGAGTTTTTGAAGTTAAATAGTGTTCTTGGAGATACAAAACTATTGCCACAGAGAAAAAAGATTGAAAATCCGCACCCTCTACTACAAACAACTGTTGAACCGAGTAAACCTGAACAGAGAGAAATGTTGCTTGATGCCCTTTTGGAAATCTCAGATAGTGATCCGCTTCTACGATATTACGTGGATTCTACGACACATGAAATTATACTTTCTTTCTTAGGGAAAGTACAAATGGAAGTGATTAGTGCACTGTTGCAAGAAAAGTATCATGTGGAGATAGAACTAAAAGAGCCTACAGTCATTTATATGGAGAGACCGTTAAAAAATGCAGAATATACCATTCACATCGAAGTGCCGCCAAATCCTTTCTGGGCTTCCATTGGTTTATCTGTATCACCGCTTCCGTTGGGAAGTGGAATGCAGTATGAGAGCTCGGTTTCTCTTGGATACTTAAATCAATCATTTCAAAATGCAGTTATGGAAGGGATACGCTATGGTTGCGAACAAGGATTATATGGTTGGAATGTGACGGACTGTAAAATCTGTTTTAAGTATGGCTTATACTATAGCCCTGTTAGTACCCCAGCAGATTTTCGGATGCTTGCTCCTATTGTATTGGAACAAGTCTTAAAAAAAGCTGGAACAGAATTGTTAGAGCCATATCTTAGTTTTAAAATTTATGCGCCACAGGAATATCTTTTACGAGCATACAACGATGCTCCTAAATATTGTGCGAACATCGTAGACACTCAATTGAAAAATAATGAGGTCATTCTTAGTGGAGAAATCCCTGCTCGGTGTATTCAAGAATATCGTAGTGATTTAACTTTCTTTACAAATGGACGTAGTGTTTGTTTAACAGAGTTAAAAGGGTACCATGTTACTACCGGTGAACCTGTTTGCCAGCCCCGTCGTCCAAATAGTCGGATAGATAAAGTACGATATATGTTCAATAAAATAACTTAGCACATTTTATATTGTTATATAAATGTGGCTTCTTGTGTAAGCAAATTAGAGAAGAATTGTTAGATGATGCTGTTGCAGAGGTAATTGTCAAGATTGTAAGTAATCCGAAATTTGCTTCTATGATGCAAGAGAAAATCAACATGAAGGTGGATACCTCTGAAATAGAAACAGAAATAGATAATTACCAAAAGGAATTGAGGAAGTGTCATTCTACAAAGTTTAAGCTAATTGAGGAAATAGATAATTTAGATGTTGAAGATAAGCACTATAAGAGAAGGAAACAGGATTTAGACGACAGACTTTATCGCATGTATGACAAAATAGATGAATTAGAATCATCACTAATTGATGCGAAAGCAAAGAAACAGACTATTGAAGCTGAAAAGCTTACAGGAGATAACATATATAAGGTTCTGATCTATTTTGATAAGCTCTATAAAGTCATGAATGATGTAGAGCGTAGACAGTTAATTACAGCTTTGATTTCTGAAATTCAAGTTTATGAAGAAAAACAGCCTAACGGACAATGGCTAAAATCAATTACTTTCAAGCTTCCGATTATAGAAGAAAATCTCAACATAGGTTTGGACAATGATGAGCACGTTGAAATGTTGTCACTTTTGAAAAAATAAAAATTAGGTTTAATTAAATTACCTAATTTTTATTTATAACTTTTTAGCATATTTATACCATTTTCTTGTACTTCAACGAATATTGAATAGCAATGTTCTTGTGAATGCATCTAATAATGTTGCTCCCGTCATCAAAAACATGAAGGTGGCAGGGCTGAAATAATGATGTCCTAATTGAATATACACAACAAGATATACAGCGCTCAATTGAACACCTAGCATAGTTATTAAAGAAACTAAATTTGGCATTCAAACATCATTGGCGCCCCATAAAGCCCTTAGGGTTGTAAGGTTTCCTATATCCAGAATAGCTCTTAGCATCATAATTCCCATAACACCATACCCAACAGAATTAATTATTTGAATATCTATTGAATAAAGTCTTAGCAAAGGCCAGGTTAATGAAACTCCTAATATTGAAAATAATGACTGTGCATAAAAGCCAAACTTTAATGCACTATTTCCGAGTTCATGAGCAGCTTCAAAATTTTGTTTTCCAATTTCGTTTGCAACAATTGCGCTTGATACATAACCCAAAGCAATCATTACTGCATTAAATATGTCATAGGAACTATTTACAGCTCTTCACAAACCAATGAATTGTTCATTCTTTCCTTCGAATCCCATCGGAATTGCTCTCGCAAGAAGATATTGGCCTATGAATCAAATTCCTGTTTCAAAAGCAATGGGAAGACCTAACTTAAGAATGTTTTTAACATATTTTGTATTCCATGTTTCTTTAATTGAAATTTTATTGTTTTTTGTTATAAAGTGATAGGAAATTGAATAATCAATTGTTAAATGAAATATTGCCCCCAAAATTGGTTCAAGTGCAACCATTATAAAAAGATCTTCATTTGAAATCTTTCAAGGTAAAAACATAATTAAATAAGTTCAACTTGCACGTACAAATATACTGGTAATTAATCCAAATATTGAAAATTTATTTTTTTCAACAGATTGTAAACTGGCCGTAAAAATAACGGCTATTGAATATATGAATATGTCGCAAGTGGTTATTCTTAAGAATTTTATAGCCATTGTTGTTTCGGAAGTTACTGAAACCTTAAAAGGATTTGTATTAACTAGGGTAATTCCATTTATAGTTCCACCGTATAGCACCTTATTAAATTGATTCATTCTAGCTAAATCATTTATAGTGATGTTGTGATTTTTCAATAATTCAATGTTTGATTTAAAAAGATCTACACCTTCCTTGTTTTTTCAACCAAAAAGAGGAATGTTTTTTGTTCCAGAAGCTTGTAAAACTATTGGTGCAAGTCCAAACATAATAAAATAGACACAAGTATTTATTATTAATGATACATAAACGGCAGACCACAAAGCTTTTGGTATGTCTCTTATTTTGCTTTGTCCAAAAAGATTTGAGCATAAGACTAATGTTCCGCTTTGGTAAATAATAGGAATAAAGGTTATCATTTTGTAAACAGTTAATATTTTTGCAATCATATAAAAATAAAAACCATTGTATGAACCATCGGCGTGATAGACTTTTATATAATGCGAAGAAGCAAGAGAAGAAACTACCGATACTAAAGCAACAAAAAGTGTCTGAAAAAAGATTGGTAAAGTCAATTTGAAAATATACTTAAAGTGTTTAACTTTAAATAATTCACTTGATTTTTCAATAAAAATACTTAAACGATTTGTATTTCTTTGTTTACTGTTTTCTTTCTTTCTCATAACTCGTTAATTCTAACATATAAATTAAAATCCATTTAAACAACTTTATTATAATGTAAAATTTATATAAATAACGCTAAAATTTGCAAGGAGATTTACATGGGATTTTGATCAAACTTAAAAGATAAATTATTTGGTACCAAAGAAGAAAGAATAGCCAAAAAACAAGCAAAAATTGAAGCAAAAGAACAAAGACAATTAGAAAAAGAATTAATTAAAAAAAATAAGTTAAATACTTATATTGCAGGTCTTTCAAAATCAAACTCTTCATTTAATGAGCATATAAAAGAATTGCAAAACAAGCATAATAAAATTGACGAAGAATTTTTTGAAGATTTAGAAGAAATGCTGATTATGTCTGATATTTCAATTAAGCTAGTGCAAATAATTATTAACGAATGTAAAAAAGAAGTTAGAAACGAAAATATAACTGATCCAAAATTAATAAACGAGATAATTGCTGATAAACTGTTTACAATTTATACATCAAATTCTGTTGTCGATACAACACTTAATATTAAAGATAATAGGTTGAATGTAATATTAGTTGTGGGTGTCAATGGTTCAGGAAAAACTACGTCAATATCAAAAATTGCTAAAAAATTAATTGACGAGGGTAAAAAAGTATTGATTGCTGCAGGTGATACATTTAGAGCAGCGGCAGTTGAACAATTAGAGATTTGAGCAAAAAGAGTTGGGGCTGATATTGTAACACCAAACGAAAACGAAGTGGATCCGGCGGCGGTTGTTTATAGGGCAATCGATATAGCAAAATCAAAAAAATACGATATATTGATTGTAGATACTGCGGGAAGGTTGCAAAATAAAGTAAATTTAATGAACGAACTTGCCAAAATCAATAGGGTTTTAGCCTCTAAAATTCCCGATGCACCTCATGAAAGTTTATTAGTTTTAGATGCAACAACCGGACAAAATGGTGTAATTCAGGCTAGGGTATTCAAGGAATCTACACCATTGACTGGAATCATTCTAACAAAAATGGATGGAACCTCAAAAGGTGGAATTGTTTTAACAATCAAAGATGAGATTGGTTTGTTTGTAAAATACATTGGTTTAGGTGAAAAAGTTGATGATTTAGCGGAATTTGATTTAGATTCTTATATCTATGGTTTGACAAAAGGTATTAACGAATAATGGAAACAATTGAAGAACGAGATTACTATGTAAAGTTGTTTGAAAAATATGGTGATTTATTACCACAATCTCAAAAACAAGCTCTACAATTACTTTTATATGAAGATTTGTCATATGGGGAATTAGCTCAAGAATTAGCAATGTCTAGACCAGGGGCTTACGACGCAGTTAAAAAAGCAAAATTACACTTAAAACAAATTGATGAAAAGTTAAAACAAAGTTAAAAAATTTCTATTTTTTGTTATTAAATAAATAATTTATGAAAATAATTAAATTTAATATATAAAATATATATAAATTATTAACCAATAAAGAGGTAAGAATGTCTTATAAAAGAGTATTGATTAAACTTTCGGGTGAGGGTTTAGCAAATAAAGGTAAACACCTTGCAATTGATTATGAATTAGTTGAAAAATTTGCAAACCAATTAAAAACAATAATTAGTAGAAATATTGAAGTTGCGATTGTTGTCGGTGGCGGTAATTTTTGAAGAGGAACAAGTGCCGAAAAAAATGGTATGCAAAGGGTTAGAGCAGATTACATTGGTATGTTAGCTACAACAATGAACGCGCTAGCTTTGCAATCAGGATTTGAGAGATTGAATTTGCATTGTAGAGTATTATCAAGCCTAACAATGGACCCAAAGGTTTGTGAAGTTTATATAAACGAAAAAGCTAAAAAATACCTAAGAGATGGAGAAGTTGTAATTTTTGCAGGAGGAACTGGACGTCCATTTTTTACAACCGATACAGCCTCAACATTATTCGCTAGTGAAATTGATGCGGATGCAATATTGATGGGAAAAAACAACGTTGATGGGGTGTATGATAGCGATCCAAAATTCAATAAAGACGCCAAAAAATTTCCAATCATTACATATGATGAATTACTAGAAAAGGAATTGAAAGTTATTGATCAAACCGCAGCATCAATGGCAAAGGAAAATAATATTGATATTTTAATATTTGATATTAATGAAGAAGATTCTTTAATAAGGGCAATAGAAAATAAAATACCTAACACAATAATAACTAGAGAAAGGGAAAAATAATGGAATTAGACAGTTATCTTTTAGAACTAGATGAAGAAATTAGCAAATCAATTACTAACTATGAAAATCAATTAATGAAAGTTGTTATAGGTAGGGCAAATCCTGTCTTAATCAGTAAAATTAAATTAGAATATTATGGTTCACCAATTTCGGTAGAAGAAATAGCATCTATAACTACATCAGGACCATTACAATTAATTGTTAAGCCATATGATATGAGTGTGCTAAAAAATATTGAAAAAGCAATCATGGATAACAAGATAAACGTTTCAATAATAAATGAAGGACACCAATTGAGATTGCAATATCCTCAAATGACCACTGAAAAAAGAGTAGAAATGACAAAGCAACTTGCTTCAATAACCGAACAAGCAAGAATAGGCGTAAGACAAGCAAGACAAGATATCAATAAAAAAATAAAAGCAGATAGCGACCTTTCAGAAGACATTCAAAAACATTATCTAGATAATATTCAAAAAGAAGTTGATAAATCAATAGAAAAAATCAATGAAATGTCAAAAACAAAAGAAAAAGAACTTTTAACAATATAAAATGCTTCGGCATTTTTATTTTTAATTAATCTTTTATTTATTTATAATTATTAAATAATGAAAAACCTAAAAATTAGAGCAAAATCTGCAATTATATTATTTAGTATATTGATTCCACTACTTATTTTAACTTATTTTGTTAAAAGTTTAGGGGTGGCAATTTATATGTTTTTTAGCGCGTGGGCTTTGTATGAAGTTATTTCACACAACCAAAACAAAAGATCTGTTAATTTAGTTATATCCTTACTTTCAACATTAATTTGAATAATACCAATTAATTGATTTTTAAAAGATTGAGGAACATTTTGAGAAATGTATAGCAATGGGCATTGAGTCTATGGCCTTGATAGAGATACATTGATTTCAATAATAAAAAACACAATATTTTTTAAATCAAATGCCTGGTCTGGTGCTCCGGGGTTGGGCTTAATATTAGTTTTCTTAATAACAACATTCTTACTTATTGTTGATGTAAAGAGTCAAAAAAATGCTAAGAATTGGTTTAAGAACTATTTCATTACAATATTGGCTGTTATATTTATTCCATCTAGTTTTAAACTAATTTTTATGTATAACGTAATGAGTTTGTATTGACTATTCATATTATTCTTAATTCCTATTGTGTGTGATAGTTTTGCTTATTTAATTGGTAGACTTATAGGAAGAAAAATAATTAAGTTTGGCTTTGCACCTCACATAAGTCCTAAAAAAAGTTGAGAAGGAGCCATTGCCGGATATTTTATAGGGGCCTTGTTTGTCTTTATATTTATGGGAATTAATTTCGGAAACTCTGATTCAATAAAAATATTAATAAATCAAAAGCAAATTATTACGGCCGTTTTTGTTCTTCCTGCAATTTCTATTATTGGCGATTTAGTATTTTCAGGAATAAAAAGAGCTCAAAATATTAAGGATTTTTCTGAATTAATAGCGGGTCATGGAGGAATTATGGATAGATTTGATTCGCTATCGTTTAATACGGTATTTTTAACTTTTATATTAATGATTTAAGTAAAATAAAATAAAAAAATCGTTATTACTTTTTAACGATTTTTTTATTAATATTAATATAATTAATAATAAAACTATTTAATGTAAATGAGGGGATAATTTATGCTTGATAAGACTTTTGTGCAATTATGCAAAGAAATAAAATTTGAATTTGATGAACAACAATTTTATGGTGCAAAAGTTTTGAGTGTTGAATCAAACTTAAATGAATGGAAATTTAAGATTGCCTTTGCAAACCATATTGACATTGAACAATACAAACAATTTAGAGAAAAATTAGTTAAAAGATTTGGCAAGGTTGAATTAATCTTGGCTATTGAGACAATTATAAGAAATAAACAAATTATTGTTGATTATTTAAATTATCTAATAGAATGTAACCCCAAGAAATATTGGCCTTTTGTAAAATATAATTATTCATGTATAACTACTTCGGCAATTGAAATAGATGAAAAAACATTAACACTTTCCTTACTAACAAATGAAATTTATGAAGTTTTTAAAAGTAATAAAGATCTTTTTAAAGAAGCGCTTGCTTCAATAGGATTTGATTTTTATAATGTAGTTTTTAATTTGGGATTAAACAATAAAAAAAGCGTTGCAAGCAAAAAACTAGACGGTTGAATTGAACAATTTAACAATTTAAAAGATAATGAAAATAACTTAAAAGAAAGCTTTTCAAAATCTAATTTTTATTCTAAAAAAGAATCAGCAAGACCAATTGAGATGGACTTAACTACTGCAAACGAAACGTTTGAAGAAATAAAAATAACCACAAAAGGAGAAGTTTTTTTTATTGAAAGCAAAGTGCTAAAAAACAAAAAAATTCTATATACGCTAAAGATTTCAGATTATAAAGAAGCTATTCTTATTAAAAAATTTTATGATTCTGAAAACGAAATACCAGATATAAAGGTAGGGGACACGATTATTGTTACTGGAAAATTGAGTGATGATAATTTTACAAAATCAAAAGTTTTAATAGCAATGGGCAAGGAATGCTTCATAAAGACAGAACCATTACAAAAACTTATTGAAGATAACGAAGACAATAAAAGAGTTGAATTAGCCGCAAGATCTAATATGAGTGCTCAAGATGGAATTTCAACACCATCTGATTATATTGATGCTGCTAAATTTTATGGTCATAAGGCTATAGCATTGACCGACCTAGATGATGCGCAAGGCTTTCCTGAATTTTATAATTATGCCAAAAAGCAAAAGGATGTTAAGCCAATATACGGCGCAACATTAAGCACTATTGATTCAGATAAAAAATTCTTCTACAATTTTAAAAATTTCAACTTAAAAGAAGAAGAATATGTTGTGTTTGACCTTGAAACAACAGGATTGAGCCCAAGATTGGATGACATTATTGAATTTGGTGCCACTATTATAAAAAATAATACTATTACTCAAAAAATTCAATTCTTTTTACAAACAAAACGTGAGCTATCAGCCTTTACTGTTGAAAGCAGTCACATTAATAATGAAATGTTAAAGAATGGTTATGAACAAGAAGAAGGCATTAAAAAAATTTATGAAATATTAAAGGATAAAATAGCAGTTGCTCACAATGCAAACTTCGATATAAATTTTTGCAAAGAAAAATTTGGCAAACTCGGCCTTGATAATTCTAAAATTTTTGGCCTTGATACATTGGCCTTATCTTATTTTTTAAACCCCGATGAATTTCGTCATTCTTTGGGTTATGTTGCTAAAAGATTCAATATTATTTATGATACAACTGTTGCCCACCGTGGTGATTACGACGCTAATGTTTTGGCTAAGATTTGAATAAAAATGATTGACAAACTTGCAAAAGATTACAATATCCTAAACTCACAGGATTTAAACAATGTCGATAGCCAAAAAATACTTTCTCATAAATTTACATATGAAACAAGAATATTGGTAAAAAATCAAGCCGGACTTAAAAAATTATTCAAAATTATTTCGCGTTCTTTAACTTCTCAATTTAAAAAAAATCCACAAATTTACTTTGATCAATTGGGCACTGATGAAGATTTGTTTTTTGGATCAGCAACAAATCAATCATATGTTTGAGACCAAGTATTCACTGGAACAAATGAAAATTTGCTAAATTCACTAAAATATTATGATTATATTGAATTACCCCCTATATCATCATTTCAATATATGATTGATAGTAACCACGTTACATTAGATGAATTAAAATTTTTGTATAAAGATTTAATTTCAAAAGCAAAAAGTTTAAACAAAATGTGCGTTGCTGTTTCTGATTGTAGATATATTTATAATTATCAAAGATTAATTCATGAAATTTATATTGAGGCTCCAACTTTGGGGGGTGGGCTTCATTGGCTTAAGAAGAGACCATCTCATCCAGAATTTAGATATTTAACAACAAGAGAGATGTTAAAAGAATTTGCTTTTTTGAATGATAGTTATTTAATTAATGAAATAGTAATTGAAAATCCAAACAAAATTGCTAATGAGATTTCATCTAATATTCAAGTTATTAAAGATAAATTGTATGTTCCAACTTTTGATAACTCGCCTCAAAAATTAAGAGAAGTTGTTTATAAGACTTTAAAAGAAAGATATGGGGATAATCCAGATCCCATCATAGTTAAAAGAATTGAAAAGGAATTAAATCCAATTATAAAGTATGGCTATTCTGTTATTTATTGAATAAGTCATAAATTAGTTAAAAGAAGTAATGAAGATGGTTATCTAGTAGGCAGTAGAGGAAGCGTTGGTAGCTCAATTGTTGCCAATCTAGCGGGTATTTCAGAAGTAAATCCCCTACCTCCACATTATTTATGCAAAAATTGTAAATATTTTGAATGAAATAGTAACAAAGAAATTTATTCAGGTTGAGATTTGCCAGACAAGCATTGTCCAAATTGCAACTCAATATTGTCAAAAGATGGGCATAATATCCCTTTTGAAACATTCTTAGGATTTGAAGCGGATAAAGTTCCTGATATTGATTTGAATTTTAGTGGTGAATATCAACCAACAATTCACAATTATGTTAAAGAATTGTTTGGCGAAGCACATACATATAGAGCTGGTACAATAGCAACTGTAGCAGAAAAAACAGCATATGGATTTTGCAAAAAATATGAAGAAGAAAAGAGAGGAAATTATCAACCTTGAAGTCATTTGTTCTTGGATTTTTTAGCATCTAAAACACAAGGAGTAAAGAGGACAACTGGACAACACCCAGGAGGAATTATTATTATTCCTAAAGAATTCGATGTAGAAGATTTTACGCCTGTAAACTATCCTGCCAACGACACAGAAAGTCCTTGAAAAACAACACACTTTGACTTCACTTCTATTCATGATAATGTCCTAAAATTAGATTTGTTGGGTCATGATGATCCTACCGTTATTAGAATGCTTGAAACATTGACAAATACAAACGCAAAGGAAATACCAAAATTTGATGCAGAAGTTATGAAACTTTTCTATACAACTGAATCATTGAAAATTAGTCCTTCAGACATTGGAGGCGAAACAACTGGAGCTTATGGGTTGCCTGAATTTGGAACAAACTTTGTAAGAAAAATGTTAAAAAAAGCTCAACCAAGATCATTTAATGATTTAATTCTTCTTTCGGGGTTGAGTCATGGTACGGATGTTTGAACAGACAATGCTGAAGAGCTAGTGGCAAGAGGCAAAAAATTAATAGAATGTGTATGCTGTCGTGATGATATAATGCGTGGGTTGATTGAATGAAATATTGAGCCCTTAACAGCTTTTCAAATTATGGAAAAAGTTAGAAAAGGAAAGGGCTTGACACCTGAGTGAGAAGAACTCTTAAAAAGTCATAACGTTCCCGAATGATATATAGAGTCGTTACAAAAAATTAAATACATGTTCCCTAAGGCACATGCTACTGCGTATGTAATTATGGCTTGAAGAATTGCTTGATATAAGTTATATTATCCATTACCTTTTTACGCTTCATTTTATACAAATAGGCCAGATGCTATTGATATTCAAATAATGAGTAGTGGCGTAGCAAGCGTTCACCAAAAACTTATTGAATTAAAAAATCGCCAAAATTCAAATGGTGAAAAATTATCTGTAAAGGAAGAAGCATTAATTCCTGTTTTAGAAATTACTCAAGAATTATATGCAAGGGGATTTAAAATTAAAAATGTTGATTTAAAACTTTCTTTGGCTGATAAATGAATAATAGATGAAAAAGATAAGGCGCTAATTCCTCCATTTAATGTTGTGGATGGTTTAGGAATTACTGTTGCTCAAACAATAGTATCTGCAAGAAAAGAAGGCTTTTTCCTTTCAATTGAAGACCTTGCTTCGAGAACAAAAGTCAATTCAAGAATTATTTCGGAATTAAAAAAAATAGGCGTTTTAGATTGCTTAGATGATACCAATCAAGTAAGTTTGTTTTAAAAATATCTTAAAATAAAAAGAAAATGAAAATTTTAATAAATTAAAAGTGATATAATAAAAGAGCTCCAATTGGAGCATAAAGATCATTGTTTATTTAAATATATAGTTCGTTTATGTCAAATTAACTAAGATTTATTTGTTAATCTTTATATAAAAGTATAAGTTCAATAATTAAAAGTTTTATATGTTTTTCAACCTCCTTCATATTTTTTATCTATATACTTAAAACATAAAAAATGTGCGTATTTTAATTCAAAGAAATCTTATACAAAAAAATTTTTGTGTAGTTGCTTTAATTTGTTTTTGTTGTCTTATTGAATATATTTTAGGTTTTTGTAAATCCTTTCTTAAAAATGTTATTTCGTATTTGTGTGTTTGCAAAACCAAAGGGGTAAATTTATATTTAATAATATTTTTCTTCAAAAGCATCTATACAAAAATAAAGCTGTTTAAAAGTTTATATTTTTTTATTAAAAAAATGTGTAGTTATTTCTTAAACAACGCCAGATAATAAGGCGTTGTTTTCATTTGCCTATTTTTTGTTTTTTAAAAAATGCTATATTTGGTAAAATTTTAATTAAATTAAATAATAATTTATATAAGAATGGAGCGTATTATGTCAAATAATGACAAGTGAAAAAATAAGAAAGTTAATTTAAAGAATTATGTTGTAGAATCAGGAAAGCCAAAAAGAGAATTATCACGTTCATGAAAAATAGCTCTAACGGGTTTATTTTTAATTGTCATTCCTTCTTTTATAATGTTTTTAATTTTAGGCATAGATGGTTGAATTATAAAATCAACAAAAAATTTATCAAGATGAGGTGTTGAGTTTCCAATAGCATTAGCAATTGCTGCAATACAAATTATTATTGTTTTATTGTTAGTTTTTAAATTTAAGGTATTTAACACTGAAGCATTAACCTTTCTAATTCCAATTTCTTTAGCCATTAATTCATTTTTGGTATCTAGTGGCCAAAGACCAGAAGATTGATATATTAGGGTTTTACCCGCTGTAGGTTTGGTATTCCTTGCAATTCCGATAATATTAATAAACAAGGCAGTTGCTAAAAGCCAAGAAAAACAAAGAAAAATTAAATTACTTGAAGAAGAACAAAAAAATAAATCATTGCTTGACTAAACTAAACTTCGAGTTTAGTTTTTATATTTAAGGAGTTTAGATGTATAAAAAAATAAGTTTAGATGATAAAGAAATCGAAGAATTAATTAACCTAGAAAATCAACGTTTAGAAGACCATGTAGAACTAATAGCATCTGAAAATTATGTTTCAGAAGATGTTATGAAGGCAAATGGTTCTTGTTTAACTAATAAATATGCAGAAGGCTATCCTAATAAAAGATATTATGGTGGTTGCGAATATGTTGATAAAATAGAAGAAATTGCACAAGAAAGAGCAAAAAAATTATTCAATGTAAAATATGCTAATGTTCAACCATATTCAGGAAGTGTTGCAAATGCTGCTGTATACATGGCAATGGTTGATCCTGGAGATAAAGTCTTAGGATTAGATTTAAATTCAGGAGGACATTTATCACACGGATATAAAATTTCATTTAGTGGTAAATTTTACGAAGCACATACCTATTCTGTTAACGATCAAGGTTTATTGGATTATGACAAGATATTGGAAATTGCAAAAGAAGTAAAACCAAAAATGATAATTTGTGGATATTCTGCATATTCTCAAATTGTTGACTTTGCTAAATTTAGAAAAATTTGCGATGAAGTAGGTGCAAAATTATTTGCCGATATTTCTCATATATCAGGATTAATAATAGCAGGATTACACCCATCACCTTCGGGTTATGCTGATGTTATAATGACTACAACACATAAGACATTGCGTGGCACTCGTGGAGCAATAATTTTAACTAATGACGAAGAAATCTTTAAAAGAGTTAATAGTGCGGTTTTTCCTGGCGTTCAAGGAGGACCTTTGTTGCACCAAATAGCGGCAAAGGCAGTAAGCTTTAGAGAAGCATTACAACCAGAGTTTATTGATTATCAAAAGCAACTATTGTTGAACTCAAAAATTATGTGTCAAACATTTTTGAACAAGGGAGTAAAAATTATCTCTGGCCTTACTCAAAATCATTTATTTATGATTGATGTAAAAACTAGTTATGGAATAACAGGAAAATATGCAACAGAAGTTTTGGGTAGAGTTAATATAACCGTGAATAAAAACACTATTCCAAACGATAGTGAAAAACCAACCGTTGCTAGTGGAATAAGAATAGGATCTGCGGCAATGACATCAAGAGGATTAAAGGAAGATGAGTTCATCATTTTAGCCAATTTAATTGATAAAGTTTTAAGAGATCCAGAAAACGAAAACTTATTATATGTAGTAAAGAAAGAAATTGCAAAATTAACCAATTCATTTCCAATTAAACGTTCTCATTTAGTTCGTTAGAAGTAGGCATATGATATTAGCGGAAATAAAAAATTTAATTAAATCTTCAATAAGTATTATTCCAGGAATAGTTGATTTTGTTGAATTAACTTCAACCAATAATATAAATATTGAAAATGATGGTTTAATCATTGAGGAAGATCCACAAAATAAAAACATTATAAATATAACAATTGGTTTGAGAATAATGAGCAATTTAAGTGCTAAAAATATCGTTGAAGGAATATATGAAATCATTCCATATAACTTAACTTCTTACGATATAAAATTAGGAACATTAACAATATATATTAAAGGAACTAAATAATGAAAATTATTACAGGAAAAGAGTGAAATAAAGCCGTATATAATGGTTCGATAAACATAAATAATAAGAAAAACGCAATTAACGCTTTAAATGTTTTCCCTGTACCTGATGGTGATACAGGAAGCAATATGTCATCTACAATTTCTTCTGCATGTAATTCTGAATGCTTAGAAGACGATTGCAGTATTGAATCAGCTTCGAGAATTGTTGCTCAAAATATGTTAATGTCTGCCAGAGGAAATTCTGGTGTAATTTTAAGTCAAATTTTTAAAGGTTTTTCTAATGGGTTTGCTTCTAAAAAATCTGCGAATGCTTTTGATTTAGTTAATGCTTTCGAAATGGCTGCTAAATCAGCTTATGGTGCAGTTTTAAAACCTGTTGAAGGAACTATTTTAACCGTTATTAGAGAAGTTTCTGAAGGTTTAAAAAACCAAGTTGTGGTTGATACTACTATTGAAGAAGTTTTTGAAATTGCTGTTAAATTAGCAAGAAAAAGTTGTGATGGTACTCCTAAATTATTAAAAATATTAAAAGAAGTAGGAGTTACTGATTCGGGTGGCGAAGGTTTATATGCAATATTGCAAGGAATGAGCGCATATTTTAGCGGTAATAAAGTAGAACCTATTGTTACAGAAGAGTCAGTTGAAAAATTTATTTCTGATGAAGAAGTATATGAAGGCGAATTTGGATATTGCACCGAATTTATTTTGGAATTATATAAACCAAAAAAATTCGACAAAGATTATTTGGTTCGTAAACTAGAAAAAATTGGTAATTCAATGGTTGTAGTAAATGATGACAAAATTTTAAAAGTTCATATTCATACCCCTAAGCCCGGTGATGTTTTAAATAGCGTAAATTCTTTGGGACAATTTGTAAAAATTAAAATAGATAACATGACTTTGCAAGCAAACAATAGTAAGAAAACCGCAAAGAAAAAACAAACAGAAGCACCAAGCAATATTGAATTAAAAAATAGCGCCATTGTTTCATGCAATACAGGCCAAGGAATCGTAACCTTAATGAAAGAAAACGGTGTTTCATATGTTGTTGATGGTGGTCAAACAAATAATCCTTCAATTCAAGATATTGTTAATGCAATAAATGCAGTCAATGCAAAAACGGTATTTGTATTGCCAAATAACTCAAATATAATTTTATCTGCTCAACAAGCCGGTACTATTATTAGAGATAAGAATGTAATTATTATTCCAACTAGAAGTCAAGTTCAAGGATTAAATGTTGCATTGAATTATAGTTCTGAAAATAGTGATGAAGAAAATCACGAATTAATGAGCAATAGTTTAAAACACATAAGATATGGAGAAATTGCTGCTGCTATAAAAGATGCTAAATTAAACGGAATAAAAGTTAAAGCATCCGATTACATGGTTATTGTAGAAGGAAAATTACAAAAAACATCAAAGAGTCTATTCAACGCAGCACAATTATTAGTATCAGAATTAGTATCTGATGATAGTCAAGTAGTAACAATAATATATGGCCAAGATGTTTCGGAAAACGATGCGTTGGAATTACAAAATTATATTGAAACAACATACGACCTTGTTGTAGAAATTTATCAAGGCGACCAAGGAATTTATCCATATTTAATCTCAGTCGAATAGGAAAAAAAACAATGAAAAAAATAGTATTTGATTTATTAAATAATGATAATGGCGAAGAACAAGCGATTTTGGGTGCAAAAAGATTTTTGCAAGAAAATTCAGATTATTTTTTAATTTTGGTTGGTCATGAACAACTTATCAAGAAACATTTATCAGATTACATTGATAGGATTGCCATAATTAATAATGAAACAATTGTACACATTGAATCTAGTCCAAGAGACGTATTAAGAACTCAAAATTCAATGCTGGAAGCTTTTAAACAACTCAACGAATCAAATGCAGATGGAATATTGAGTAGTGGAGATAGTGGCTCGTTAATAACATTATCATCATTGAAAGTTAAAAGGCTTCTTGGGATTGAAAGACCAGCATTCATGCCTATTCTACCAACAACAACGGATAAAAAAATGTTATTAATAGATGCGGGGGCAAACATTGAAATCAAACCAGAATATTTAAGCCAATGGGCTATGATAGCAAGTGAATGATATTCGATATTGTTTGGAAGCAAAAAGCCAATAGTAGGACAATTAAACGTAGGCGTCGAAGAATACAAAGGTTCTGAAATAACAAAGAATGCTAAAAATATATTAAAAGAAGTTTCTAAATCTGCTAATTTTGTTTTTAATGGTTATGTTGAAGCAACAAATGCCATTAATGGAGATATAGATATATTAGTGGTTGATGGATATGGCGGAAATGTGTTTCTAAAAACATTAGAAGGAACCTTTATTGCTTTTTCAAAATTATTGAAAAATATTTTATTAAAAAATTGAAAAACAAAATTAGCGGCATTAATTTTAAAAAGGCATCTAAGGGAATTTAAACAAAAATTTGATTATAGAAATACAGGCGGAGCGTTTATTATTGGGCTTGAAAAAATTGTTGTAAAGGCTCATGGGGGAAGCGACGAATTAGCATTTTATAACGCCCTTAACCAAATAAAAATAGGTATAGAAAACAATATTTCAACAATTTTAAATAAGAAATTCAAAGAGGCTGATAATGAAGAATAATTTTGAAGAAAATTTACAAAATGCATTGCTAAAATTTGGTATAAATTTTCAAGAATTAAATCAACAAAAGAGACATTATTTTTATAATGCTTTTACTCATAGTTCATACAGCAACGAACATAAAAATTTAGAAAGTTATCAATATTTAGAATTTTTAGGTGACTCAGTTTTAGGTTTTGTGGTTTCTGATTTTATTTTTGATAATTATAAAAATTTCGGTGAGGGAAAGGCAACAAAATTAAAAAGTGAAATTGTTGACAAAAATGCGCTTGGATTTTTATCGAAATATTATGGTTTTCCAAATTGTTTGAGAACAGGTAAAAGTGCATTTGCAAACGGAAAAGGAATCAAATCAGATTCTGATCTAATTGAATCATTCATTGGTGCTTGTTATAAGGCCTACGGTTTGGAATTTACTAAAAAAATTATTTATCATTTTATTGACAAAATATTAAAAAAATTTCACAATTCAATCAATGAACACCCTAAAACAAAATTGCAAGAACTTGTACAAAGCAATTGAGGTTGTAGGCCTAGATATGAAGATGAAGCTTTAAATAACGGCAAATTTGAAAGTAAGGTCTTTATTAATAATAACTTATATGGTGAAGGTATAGGTCAAAGTAAAAAAGAATCCCAAACAAAAGCAGCAAGTGATGCTTTAAAAAAAATGGCAAAAATTTAATCTGAAAGGAAAGAAATATTTATGAAATTAATTCAAGTTGAGGCACACGGTTTTAAATCATTTGCAGATAAAGTAACCCTAAAATTTGACGGTGGAATTGTTGCAATAATAGGACCAAATGGGTCAGGAAAATCAAACATAAACGATGCAATCAGATGGGTGTTAGGTGAAACTAGTTCCAAAGTGTTGCGTGGAGATACAATGGAAGACGTTATCTTTTCAGGTTCTAAGACTGAAAAAGAAATGGATCGAGCAGAAGTAATTTTAACATTTGACAATAAGGATAGATCTTGTGATATCCCATATGATGTTTTTACTATTTCTAGAGTATTAACTAGGGGAAATGGTTCAAATGAATATTACATTAATGGTGAATTAGCAAGACAAAGAGATATAAAAGAAATTGCAATGCAAAGTGGTATTTCAAAATCAAGTTTAGCAATCATAGGTCAAGGAACTATTTCTAACATTGCCGAATCTACTCCTGAAAAACGTAGAGAAATATTTGAAGATGCTGCCGGAACATCAATGTACAAAAGCAAGAAGATTGAAGCGCAAAGAAAATTAGAAAAGACCCAAGAAGCATTGGAACAAATTTCAATATTAATTCAAGAACAAGAAAGACAATTAAAGCCATTGCAACGTCAAGCTGAAAAAGCAAAAATTTATAAAACAAAAGCAGAACAATTAAAAGAAGTTGAAGTGGCATTATTGGTTCATGATTTCATGGATTATTCAGATAAATTAGAAAAATTAAATGTTGAGGGGCAAGATTACAATTTAATTAAAGAAGAATTAGAAACTAAAATAAGAGTATACAACGAAGCAAGCGAACAAAAAAGCAAGTTTGTTTTAGAATTAGAGAATAACATTAAAAAGATAAGCGAAAGATTAAGCGAAATTAGCGAAGAAATTACGACTCTAGAAATAAGAAACGCAAAAGAAGCAAAACACCGCGAAATGCTTTTAAGTGGCGAATTAACTATATCCCCAAAAGAAAAATTAGCAGCAATGAAAGAAGAATTGAATGCATTAAATACTACTATTTTAGGGTATAAGACATTCTTAGAAACTAAAAAGAAGGAATCTGAGCAATTATCTCAATCAGCTTCAATTAGATCAACAAAAATAAATGATATAAAACGAGAATTATCTTTTAAAAAAGAAGAACTTAATAAGATAAAATCAAAATTAGATGTTATTGAAGAAATAAAATCTAAAAAAACAAATTTAGCTAAAGGAACACATAATATTGTTGAAAATTCTCATTTATTTAGGGGATATAAAGCATTGGTTAGTGAATTAATTGAAGTTAATGAAAGATATGCCTTAGCTATTGAAACAATTCTAAGCTCCGCTATGCAACATATAGTTGTTGACAAACCAGAAACAGCAGTTGAAGCAATCAATTTCTTAAAATCAAATAATGGCGGTAGAGCAACTTTTATTCCCTTATCATCAATTAAACCAAAATTCGTTCCTGAACAACATATTGTTGTTGCTCAAACCCAAAAAGGTTTTCTAGGAATTGCTTCGGAACTTGTTTCTACAGAGGAACAATATAATGTTTTAAAGAAATTCTTACTAGGCAATGTTTTAGTTGCAGATAACATTGAAAATGCAAATAAATTATCTAAATTGCTAGAAACTCGTTACATGGTCGTAACGCTAGATGGCGACATCATAAGAGTTGGTGGGGTTCTTTCAGGTGGACAAGCAAGTCAAAATGTTTCCACATTAGCAGCTGATGAACAAGTTAAAAAATTACAAGACCTTATTCCAGTAATTAATGCAAAAATTTCACAATTATCAAATGAGTTGTTTGAATTAGAAAAAGACCAAAACAAAGAAACATCGTTATTAACAAATATTTCTCTTGAAAGAGCAAAGATTGATAATTTATATAGAACAACATTGGATAAGTTTGATTCATTGCGTGAACAATATCGTAGTGCTTCGCAAGAAGAATATGAAGCAGAAAACAATAAAAAAATTGATATAGTAGCAGCAATTCAACAGTTGATGCTTGAAAGAAGTGATTTATCGGCTAAAAAATCATCTCAAGAAGATATTTATCATAATCTAAAACAAGAATTACAAAATCTTACCAATCAACGCTTTCAAGTTAGTGAAGAATTAAATAATTTGATTAAAGATAACGCTAACAAAATAGCAGAAAAAGCAAAAGCAGAAACAATTATTCAAAATGCAAAACAAAGATTGTCAGAACAATATGGAATGCTATTTGAAGTGGCAAAACAATTTTATAATCCAGATATAGATTTTGAAGTAGCAAGAAAAACAGTTGCTGATTTGAAATTAGATATAAGAGAGTTAGGGCACGTAAACCTTGATTCAATTAACCAATTAGAAGAACTTGAAACAAGATATAAAGAAGTAAAATCTCAAGAACAAGAAATAATTTCAGCAAAAACCACCATTGAAGATGCAATATCAGAAATGGATAAAATTATTATTGAAAGAATGACGCAAACAGTTACATTAGTCAATGCCGAATTCAAGCATGTGTTTGCAAAAATGTTTGGCGGAGGTATGGCTGAAATAAGATATACTGACCCTGAAAACATTCTTGAAACAGGAATTGACGTCATTGCTCAACCTCCAGGAAAATCTATTAAGAATTTAAAATTATTTAGTGGGGGTGAAAAGGCACTTATAGCTATTTCATTACTATTTTCAATATTAAAAGCTAAGCCATTGCCATTGTGTATCTTAGATGAAGTTGAAGCAGCCCTAGATGAAGCAAACGTAATAAGATTTGCTGAATATTTACACACTCTAAAAAAAGATACTCAATTTATAGTGATTACTCACCGTCAAGGAACAATGGAAAGAGTAAACAAACTTTATGGTGCAACGATGCAAAAAAGAGGGGTTACAACATTTTTTGCAGTAAATCTTTCAGATGCAAAGAAAACTATTGAAAACAACAATTAATTTTTTTAAAAAACGTTAGCTTAAAAAAGAGCTAGCGTTTTTTAAAATTTTACTTAAATAAAAAAATTTACCGAAAGGTTATATTTTTTGATTTTTTTAAAAAATTATTACAAAATAAGGCCTTTTTTTTAAGTTTCTAATTTTTAATATACAAAAATATACAAACATTATATTTCTAATATTTAAATTTATCCTTAATTTAAATTAACAATTAAAAAAGAGGTAAGAATGAAAAAGTCTAAGAAAATATTATTAACAATAGGAATTGCATTTCCACTTTTAGCTTCAACTCCATTAGTTGCTGCCGGATGTGTAGAAACAAATAAACCAGAAGATAAAAAACCAGGAACAACTCCTGAAAAGAAGCCAGAAGATAAAAAACCAGGAACAACTCCTGAAAAGAAGTCTGAAGGTGAAACCACAATAGAAAATATCATTAATAAACATAAAGAAATTAAAGATGATGAATTAAACAATACTAAATCATTATTAGATCAATTTAAAAAATCAATTGAAGAAAGAACAAATCAAGCAGTATTAAACGAAGCAAAGAATCAATTAAATTCAGCTTTAAAACATGCGGTAAAAATAAATACAAAGTCATTTAATTTTAACCAAAAATTTAATTTTAATAAAGAATATGTGAAGGGATTAGAAGCAACTAAAGGTAACGATTTGAATGTTATTAAAGAAATTACTTTCAAATTAAATAGCTTTATAAATTTAGCAACATCACAAAAACCAAAAACGGCAACAGAAATTCAAAATCATGTTGAAGGCGAAGTTCATGGATTAAATGATTTAATATTAAAATTGGATGATTTTGTTTCTGAATATGTTGAAGAAACATTTAATGATATAGATGAAGCAGGTTTAATTAGCTCAATAGATAATTGAATCGACATGGTTAAAGATTTTATTAAAGAATCTAAAAATCCTTCTGAATCGCTATATTTTAAATTAAAATATTTGAAATCGCAAACAAAAACATTTTATGAACAAAAAATGGAATCATGAGCAAACAAAGTATCTGATCTACTAATTGAAAAAGGATATGATCTTTTGGATAAAATAGCACATATTGAAACATATTATAGTGAAAAGAAAATGCCAATGGTTGAAAGCCTTGCTAAATATCGTGAAGAATTGGAAGATTGCTTAAATAAATTTGCTGAACTAGATGAAACAAATGCCGTGGAAAAACAACTTGAGTTGAGTAAAAAACTTTATGATTTAATAACAAAAATTCAAAAAGAATTAAACAACGAATATAACGAAGACCTTAAATAATAAATTAAATTAAAAATATTTGAAACAAAAAGGTAACAAAAGAACCTTTTTGTTTTTATTAATTAATTTTTAGTAAGCTTAAATATAAAGGAATTTTATTAATATTTTTGATATTAAAACCTAAAAACGCAATATTTTCAATATTTTTTAGTTAAAAGTTAGATAATTTTTATAGTGTATTGTTAAACAAATACCATTTATGCCAATGACTAATGTTATATTACAATAAACAAGGAGAGCCTTATGTACGGGTTAGTTTTTTATTTAGATAAAGAAATATTAAAAAAAGAATACTCAGATAGTAATCATAAAGAAGCTTATGAAAAAGTAAGAGATATATTGAGGCACTACGGATTTCATTGATTATCAAATAGCTTTTATTTCTCGAGGTCAATTAATAATTTATTACAAGTCTATAAAGCAATAGAAGAATTAAAGACAATTTCGTGATTTATTAAATCATTAGTTAGCCTACATATTTTTAAAATGGAAGATTTATCAGATTTTACCGAATATTTAACAGGTGAAAGCGAAAAAATAGAAAAATAATTAAAAAACTTGTAATGTATTAAATTGCAAGTTTTTTTTAAATTAATTGTAGTTAATCATTTGGATTAAGATTAAATAAAAGATAGCAAAAGATCTCAAATGAACAATATTTTTTATAGTTAGTTTGTTGGAATATTTTAATATTTAAAAAGTAGCAATTGTGCTACTTTTTAAAATTGATAGATTATTAGCTTAATTTATATAGATGATTTGGGTTTTCCTTCAAAGCATCTTTTAAAAATGATTTAATTTCATTGTTTTCAATTTTAGAAACAAGAGTTTTAGCAGCTGATTCCCCAGCACCGTAAAAGTCAAAAGATTCAGGACTAAATGATTTCATTGTTGAAGGAATTTCTATTGTTTTTATTTGATTATCTGCCAAAGCTCCACTTTCAACTAATTCAATGTTTTTTAAGTTTATGTTTGTAAGCTTATTTGAAGCAAAAACACCTTGTGTAAGAGTTTTTAAATAAGGTAAATCTTCTAAATTTACAGATGTGATGCCACTGTTTGCAAAGGCTTTTTGTCCTAACTTATTTAAAGATGCAGGCAATTGTAATTTTTTAATATTTGTATTTGAAAAAGCTTCAGCATCTATTTGAGATAAGTCGTTGCATTGAGAAAAATCTACTTCTTCTAGGTTACTTAATCCATAAAAAGCACCTGCGCCAATTGATTTTAATGTTTTTGGCAAAATAATTTTTCTAATATTTATTTTTTTGTTGTTATTTGAAGAATTAGTTGTAACTAATGTTTTTAATTCGGTTGGTAAGCCGGCACCATCGTCTGCTAAATTTTGCATAACTAAATAAGAAAATGCACCTGCGGGTATTGAAGTTATATTGGTTTTTGATAAATCTAGCAACCCTGAATCAGCAAATCAAGTTTGATTGTGATTGGTTGAGTTCTTAGGGTCTTTTAGCGGTCCTTGTACATAGTTTAGAGCAAAAGCATTGAACTTTTCTCCTCCACAAGATATTGCTGTTAATCCAGCAATAAATGGAGCAACTAAACCAAAGCCTAAAAATAAAAATTTTTTGTTCTTTTTCATAGTCAAAATTTTACCATAATTTTGTTATTTTAGTTTTTTATATTAAATGTCAAAAATAAATTACTTAATTTATATAATTAAATTAATAAAATCATGGAAAAGTTTAAATTAGTTTCAAATTATAAGCCTGCCGGCGACCAACCACAGGCAATAAATGAATTGGTTCACGGTGTTTTAGAGGGAAAAGAGCATCAAGTTTTATTAGGCGTAACTGGTTCAGGAAAAACATTTACCATGGCCAATGTCATTAATCAATTAAATAGGCCTACATTAGTTATAAGTCATAACAAAACATTAGCAAGTCAACTTTATAGCGAATTAAGGGAATTATTTCCCGAAAATAGAGTTGAATATTTTGTTTCGTATTTTGATTTTTATAAGCCAGAAGCATACATTCCTAAGTCTGATTTATATATCGAAAAAACAAGTAGGAACAATAAAGATTTAGAAGCAATGAGAATGTCTGCTCTTAATGCTTTAAGTATCCGAAGAGATACTATTGTTGTTGCATCTGTTGCTGCAATATATGCTGAATTTAACCCAAAAGAATATATTCAAAGTTTTATGCCTATTGAAAGCCATATGAAAATTAAGTTGCGTGATCTTTTAACCAAGTTGGTTAATTTAGGTTATGAAAAGCATAATGGTGATTTATCTTCTGGACAATTTTTTAATAAAGGGGAAGTTATTGAAATTTGTCCTGGATATACTTCAGAAAACAATATTCGTATTGAGATGTTTGATGATTATATTGAAAGAATTGCATTAATTAATCCTTTAACAAAGTCAATAGTTGAAGTGCGTAAGTCGTTGACTATTTTTCCTGCAACTACCTATAATGTAGAAAAAGGTTATATTGAAAATATTTGCAATAGCATTGAAAGCGAATTAAAAGAAAGAGTTGCCTATTTTAAATCACAAAATCAACTTTTGGAAGCACAAAGGATTCATGATAAAACTTTAAATGATATTGATTCATTAAAGGAATTCGGTTATGTTAAAGGCATTGAAAATTACGCTCGATATGTTGATAAACGTGAACCCGGACAAAAACCATACACGATATTTGATTATTTGCCAAAGGATGCAGTAATTTTTGTTGATGAAAGTCACCTTATGATTCCTCAAATAATAGGAATGTATGAAGGAGACCGTTCTAGAAAAGAAACACTAATTAAATATGGTTTTAGATTACCTTCTGCATTAGACAATAGACCCTTAAAATTTAATGAATATGAAGAATATAAATTTCCAAGAATTTATATGTCAGCAACTCCTGGTGACTATGAAATAAATAAAGCAAATGGTGAACTAGTTACACAATACATTAGACCAACAGGTCTTTTAGATCCCGTTATTGAAATACATTCAAAAGAAAATCAAATTCAAGACATTCATGATTTATTAAAATTACAAATAGAAAAAAAAGAAAGAACTTTAATTTTGACTACAACTAAAAAGAATGCTGAAGAGCTTTCTTTGTATCTTTCTGAATTTAAAATCAAAAGCGCATATATTCATGATGGATTTAAAACCTTTGAAAGAAATGAAATCTTAAAAGGTTTAAGAATGGGAAAATATGATGTTGTAATTGGAATTAATTTATTGCGTGAAGGTATTGATTTGCCAGAAGTTAGTTTGATTTGCGTATTGAATGCAGATTCTATGGGGCTAATGCGCGATGCTAGAAGTTTGATTCAAATAGTTGGCAGGGCCTCTAGAAACGACCATGGAAAAGTAATATTTTATGCCGATAATATTACAACCAGTATGCAAAAAGCAATTGTTGACAATGAACATAAAAGGACGTTGCAAATTAAATATAATAAAGAACACAACATTACCCCTAAAAGTATTATTAAACCAATACCCGCACCTATTCAAGGCAGTATTTTATTAGAAGATAATAAAGCAACAAAAGAAGCCAATAGAACTATTCATAATAAAAAATATATTGATAATATGATTAAACATATGAAAGAACTAGCAAGGGCAAAAAAATACGAAGAAGCTATTGAAATAAGGGATTATTTGATTGAATTAGGTGTCGAATTTGATGATTAACAAAAGACTTTTACGTCTTTTTTTAATCATTTTTTTGTTATTTAAAAACATAAAAAAATAAAATAAAAGTTATTTTTTTTATCAAATTTTTAGTTTTTTTTAACCAAAGCGTTTTTTTTTTTTTTTTTTTTAAAAGAGCAAAATTATTTTTAAGGCAAGTAAGGGAAGTGATGAAAAAAACCAATATAAAATGGAGTTTTTTTATACTATTAGCAATTATTTTTATAATTGCTTTTGCTTTAATATTAACTAGCTGTGCCTCAACTGATCCTAGAGATGCACAGTTTTATATTAGGGATGATAGCTTTTATTACAATAGTAGCGCAAACATAACAAAAGTTAATTTTCAAGTAAGTTTTGAGAATAATTCAATATATAAAATTGTTAAGAATTCAATAACATTTGATTTGTATGATAATGGAAAATATGTATTTCAAAACTCTTTTAATTGAGATATAAACATAAAGCCTTATAGTACATTAACAAAGGAATTATCGTTTTCTGTTAATGGAAAAGTAGATAGGCTTGAAGTATTAAATTGAGAGATAGTTAACGCTAGTATATGGCAAAGTTATAAAGCTTATTTCATAACCATTATTGTTATAATCTCAATATTACTAGTTGTTTATGCAATAGTACAATTGGGTTATAACATTAACGAAGGATGTGACCCTGAAGATTCATTTGCGGTAATTATTTGAATTCTTACTAGTGGTTGGTGAAGTTTTTTAATTGCAATAGGTGTTGCAATATTGCCTTATTTAATTTATTCAGGTGTTTGAAAAACTTGAAGTTGAGTACCTCCAATAATCATAGTAGGAGGAGTAGTTGTATTAATAGGGGCTTGTTTTTTAGTAAGTTTTATATATTTCGTTATATCTGCAATTCATAGCAGAAGAGCCGAAAATCAAGTGATTAGTTATAATTGCGATGAAGTAATGAATGAATACACTAAAAATCAATTAAATCAAGTTTATAAGCATGTGCTCTTAGATATATGCAAGGCTTCTGAATTTAAAGTTGATGAAAAAATAAAAAAAGAAGAATTAATAAATTTAATTTTGTCAAGAAGACCAGATTTTAAAGAAAGCGACAACACAAAAAAATTTTCTAAACCAAACGATAAAGAAATTAAAACATTAAAAAGCCCCCCCCCCCCTTCTAAAAAGCCGATGTTTGAAGACATTGTGGGTTTAAATAAAGCAAAAGAAGCGTTTATCGAAAAGATTATTCTTCCTTTTAAACACAAAGAAATATTTGAAAAGTTTAATAAAAAAATAGGTGGCGGCATATTACTATATGGTTTGCCCGGTACAGGAAAAACAATGTTTGCAGAGGCTACTTCTAATGAATTAAATGCTTTATTTATTCCAATAAAGTGTTCTGATATTAAAACTAAGTGATATGGTGAATCAGAACAAAAAATACATAAAATTTTTGAACGAGCAAGGAAACAAGAAAAAGCAATAATTTTCTTTGATGAATTTGAAGCGATTGGAACTAAGAGAATGGACAATGCAATTAGTGAAGGCAACGACATAGTCCCACAATTGCTATCCGAAATGCAAGGAATTGATAATTTAAAAAATGAATCAAATATTATTGTTATAGCAGCAACAAATAAACCATGAATGATAGATTCGGCATTTCTAAGACCAGGAAGATTTGATGAAAAAATTTATATTCCACTTCCTGACTATGAATCAAGAAAAAAGATGTTTGAGATTCAATTATCTTCGTTGCCAATTGCCGATAATTTCGATTTTGATTATTTAGCAAGCATAACTGAAAATTTTAATGGCGATGATATAAAAGAAGTTTGTGAAAAAATAAAAATGAGTGCTATTAAAAAGAATATAAACAATAATCAAGAACATAAAATAGAAATGGAAGATGTAAAACAAATAGAAAAAGACATTAAATCTTCTGTTAATCTTCAAGATATTCAAAAATTAAAAGAATTTGAAAAAAATTTTTATTAAAAAATAATCTATATAAATTTTAAAAAGGAGAATATTATGCAGGTAAAAAATGCCAAAAAATTTGCGTTAGCTACTATAATATTATATTTTATTTGTGTAGTACTTTTAATACCAATGATTATATTAAATTTGATGCAAGATACAATAAAGGAAAATCTTTCTTTGTTTTCGGCGATAGCTTTGCTTTCGTTTGCATTAGAATATTTAGGAGCTGCTATAACGTTCATAACGTTTTATATAATTACAATTGTAAATGGTTTTTACTATAAGGAAACGCTTGGATCAATATTAATGTGCGTAGGAATTATTGTACCTATTGTAGGAATTATTGGAACGTTTTTTGTTTATTTTAATCTTAAAAAAATACCGGTTGTTAAATTAACCACACCAATCGTTAAACCAACAGTTGATACTCCAAATCCATCATGAAATGTCTCAGATAATAATGAAGACGAAGATATAGAAGAGGATGAATAAATGAAAAGAAATAAAATAAAAGAATCTCTAGCGATTGTTATATTGCTCCCTATTATTACTGCCTTGAACTTACTTTTTGTATTTACTAGAGGATGAATAATTGATTATAATGCATATCATAATACCATTCCTCTTTGATTAACTTTATTTTTAGCTTTAAATGTAGCTGTTTTAGTTCAATACATAGTTGTTATTGTTTATGGATTTAAATTGCCCAACAAAACAGGCGCAATATTAATGTGCACAGGATTTTTTTCATTTGTAATAGCATATGTTGGATGCATAATGGTTTATATTGAACTTAGGAACAATGAAGAATTAATTTAAAAATAAAAAATAGACTCGTAATTAAAAAGGAATTACGAGTCTATTTTAAACCAATTTTTACTTTTTGTATTTTTCATCATCAATAATTTTTTTAATTATTCCTTGCTCCAAATCAAAAATATTGTAGATTGTTGATAGTTCTTGAAATGTTTCTTTTAAAACACTTTTAGATGCTATTCATCCGCAACCATCAGTAAATCAAAGAAATGTAATATTTCCAGTCTCATCAAATAACCTTGATAAAATCCTATAACTTCTGGCAACCTCATTCAATTTTGAACCACTTGTATTGTAAAAATTACATTCGCATACAAAAATATGATTTTGATTATTTATAAAAATAAAGTCAAATTTTTTATTTATATTTAATAATGTGGGGTCAATATTAATATTCGTTTTCTTTTGAAAGTTATTTAAATCAATTTGCTTAAAATAGTTAACATTTTCAATTAAATTTGAATTTATTAAATAATGTTCAACAAGGTTTTCCATCATTGAGCCTGTTCTGTTTTTTCTTGTATTTGTATCAATTCCCACTTCAACACCAGTTGCATAGTCAACAAGGCTATTGTTTATATTATTTCTTATTAATTCAAACAAACCTGTCTTTTCCATAAATAGTGTATATTCTTCTATACTATTTAGCTTTTTATCAAAATTAATTAGAATTTCTTTTTCAATTCCAACTCGAATATTATTACCTCTTATTGCTAATAAAGTTGGTATGGCTTTTAATACATTAGGATACATTTGTATTAAATTTTTAAAATCTGCTTTTATATTTTTTGATTTAATTAATGAATTTAAAATGCATAATTCAATTTTTATTTTTTCAATATTTTCATAAACTTTTTGGAAATTCACAAAGTAATCTCAATTACATATTCTGTTTTTTGAAAGTGTTTTTAAAAATTTTTCAAATCTTATTTGCATAGAACCTCTTTATTAAAATTAGTAATGATTACCTCTTCGACATCGCCTCGATTTTTACCATTTCTATTAATGTTTCTTCTTGCTTTAATTATATTAATATTAAAATCCCTGTATAAATTGCAAACAAAATCAGTTTTGTGATTACTTAATATTACATAAACGCCCTTTTTTGACAGTTCCTTAAAAACATTAGCAAGTTTAATTTGCTCGTTTTTTCCAAAGGTTTCATTTGAATAAGAAGTAAATGAATTTTTATTTTCTAAGGTATCATAAGGAGGGTCAAAATAAACCAAGTCGCCAGCTTTTGCATCTTTTATTGCATCTTCAAAATTTAAATTTGTTATTTTGTAATCTACATTAGAAAAATATTCAAAAATAGAATTATAGTTTTCTTTATTATAGCAATTGACTTTTTCTTTCTTTCCTGATGGAACATTAAAAAAACCATTTTTATTTACACGATATAAACCATTAAAACATGCTTTATTCAAATAAATAATTCTGGCAGCCTTTTCTTCATCTGACAAGTTTTTATATTCGGGTTGTCTATCTAAATTTCTTATTTGATAATAGTATTCGTCGCAATGCTTACTGGTATGAAGGTCTAAAAGTGATTTTAATTTATCAAATGATTCCTTCTTTGTAAAACACTTGTATGCTAGAACCAGTTCTTTATTAATATCATTTATACAAAAATTCTTAGGCAAGAGACTAAAAAGTAATGAACCCCCACCAATAAATGGTTCATAGTAGTTATTAAATTTTTTAGGTATAAAATTTAATAATTTTTCTAAAATTTGTGTCTTTCCACCTGCTCATTTTACAAAAGGTTTTATCACTTTTTTCCTTCCTTAAAATTAATTATATCTTTTAAATTCAAAATTAACTTCATCATTGTTATTTACTAAATTTAATGAATAGATTAGATTCTTTGTAGTTTGTTTTTCTGTGTCGTATCATTCCATTGTTTTTCTAAAATTTGTTACATTGCGTTTGTTATTTTGTTGGTGGGTTCAATATCCGTTACTTCCAAAGGTTAAAAAATAAGAATTGTATAAATCAATAATTTCATTAATTAATAATTTTGCTTTGGGCGAATTAGTATTTTTTATTCCTTCTATCAATTGCACAACTTGTTCATACAATGATTTATTTTTGTCATATCCAACGTTTGCAAATAATGATAATTCTTTCATTTTTTTATCTAAAATATTTTTGCTTGAAACAATGCTTGATATTTCATCTGATTCAATTTTATTATTATTATTTTTATCAACTCAAAGTTTTAGATTTAAATAGTTATTTTGATTTGTATAATCTTTTATTTCATTAATTCCAATTGAATCAGAAAAATATGAAACATATTCATAATTTTTAACATTATTTGAATATATGCTTTTTTCATTTAGCAGATTTTTTGAATAAATTTCATTTCATCTGCTTTTGGCAATAAAGTTGTATTTTGAGGTATGAATTCTAAGTTTTAAAGGATTTTTATTTTCTTTTTGAAATAGTAAATAGGTTGGATATTTTGCTTCCTTGCTATTTGAATCTATTAATGAATTTTCTAAGTTTAGAAATCCCCCAATATTTATGTTTGAATATTGAGAGTTAATATTGCCATTATGATCAATATATCAACTGCTAATATCAGATCCCATATAACTAATAGGTTGACCAAAACCCATTAAATCAATATACGCTTTTAATAACGCCTTTACTTGTTGATTTGGTTTATAAATGTATTTTCCTTGCTTATTTCTAAACAAGAATCTATTATTAATATTTTTATAATTTTTTAATTCATCATCAAATACTCAATTGGCTGAATATATTGCATATTTATTATAATAATTGGCAATCATCTTTAGTGTGTCATCACCAATTGAAGATAGAAAAATATCATCATTTTTTAATTTATAATATAAGAAATTCAAAGAATCATCCAAAGTTAAATGACCGTCAAACATTTGAAAATTTTCATGTGCTGAATACGAAAGTTTTACTAGTTCTCTTGCAAAAAGTTCCTCAAATGAAAACAAGTATTTTATTTGATCAATATTAGTAGAATTTGCATAATAAACTGTATTGTTTGTTGAATTATTAAAATAAAAGTTTTTATTGTTTAAAATATTGTATTTTTCAACTTCTTTGTTTGTTAAGGCGAGGTTAGCTAACTTGAATAAGTCATATTGACTGAATTTTGAATAAACTGGTATTTCTTTGTTTTTAGGATTAAATAATTCTTTATTGACTAAATATTCATCGTTTTGCATTGAACCATCGTAGTTTAGCGTTTTTCTAAATTCGGTTAAAAATTTGTTGTTGTTGCCATTAAATTTATTGATTATTTTGTTTAGTTCAGGAGTTGCTAATTTATAGCTTAAATCATTATTGGCAAACTCATCATTTTCTCTTATACTTCGGTTGTATGTATTATCAATGTGGTGTGCGTATTCATGAAATAAAGTATTAAAAACTAGCTCAACTCTCTTTTGCAAACTTCATGATTGGACTTGTTTTATTCCCCCAGAGATAAAATGATTTATTTGAATAAAAATGTCAAAAGCTTGTGGCAAATAAACACCATTAACATCTTTAATATTTGAAACATTAGGTATTTTTTTATTTATATAAACATGATTTAAACCATTAATTTCAGGACCATAAAAGCCTCTTTTTAAAAACTCTAAATTCAATAATTTTCTTCCTTCTTCACCCAAAAAATAAATAGGTTCATTATTTTTTGTTAAAGCAAAAGGATATTCAAAAATACTTAAATTTCCATATTGATAATTCTTGTTCTTTAATTGAACATTGTTTTTGGCAATTGTTTTTGCATTGAAACTTTGTAGATATCTTTCTTCATTTGGCATTGAATAATTTTTATTTTTTTGGCCATCAAATAAACCCCCACCATTGCCATTTGAATAATTAACATAATTAAAAATGTAATATATTACGACGCCAATAATACCTGCTGTTAATATAAAAGCCAAGATTATAATAAATATTGCCCATTTCGGTAGAAATGCTTTTCTACCTTGTTTTTGCTTGCTCATTACTAAACTACACTCCTAACTAATTTTAATAACTAATATTTTATAATTTTAATATTATAATTAAATAATATGTTGAGAATAATAGCAGGAAAATATAGATCCAGAATTCTAAAGCAACCTTCCAAGTCAATAACAAGACCAACAATAGATAGAGCGAGGGAGGCAATATTTTCTTCAATTCAGTTTAACATTGAAAATAAGAAAGTTTTAGATTTATTTTCGGGATCGGGAGCATTTTGTTTAGAAGCTCTATCAAGGGGCGCAAAATTTGCGGTATGTGTTGATAAAAATGCCGAGGCTATTAAAATAATTAAAGAAAATCAAAATAGTTTAAAAGAACCAAATTTAAAAATTTTTAATATTGATGCATTGAATTTTTTAAATAATAATGTTGAAGTATTTGATTATATTTATTTAGATCCCCCATTTGTTGAAAAAAAATTGCTAGTTGAGTGTTTAAACAAAATTATTGATAAAAAATGTATTGATAAAAGTGGGACAATGATAATTGAAACAGATTGAGAAGACTTCAATTATAGCTATTGCGGTTTTGAAATAACTAAAAGCAAAAAATATGGTAAAATTTATTTCTACTATATTAGCAATATAGATGATATGGATTAATGAATATTAAACATTTTAAAATCTTATCAACTTAAGATTAGGAGTTTATATATGAGTAAAAAATTAATAATATTTACAGGACCAAGTGGCGTTGGAAAAGGAACAATTGAAAAGCCCTTATTTGAAGATAAAAGTCTAAAGCTAAAATTGTCAGTATCAATGACAACGAGATTACCACGAGAAGGAGAAATTGATGGTGTACACTATTACTTTGTGTCAAAAGATACTTTTAACTCTTGCTTAGCAGAAAATAAGCTAATAGAATATTCAATGCACTTTGATAATTATTATGGAACATTATATTCAGAAATTGAACGTATTAGTGAACAAGGAAAAATTCCATTTCTTGAAATTGAAACTAATGGAGCTAAACAAATTTTAGAATATTATAAAAAACGTAACAGATTGGATGAAATTTGTTCAATATTTTTAATGCCTCCTTCATATGGAGAATTAAGAAAAAGAATTGAAGGTCGCAATACCGAAACTGAAGATCTAATTATTAAAAGAATGAAGAAAGCAAAAGAAGAAATAGGCTATTCTACAATATTTCATCATGTGATAGTTAATGATGATTTAAATGTTGCACTTGAAGAAATCAAAGAAATAATCAGATCAGAATTTTCAGATATTATAAACAAAAAAGAAGTTGCAAGTGAAGGTAGAAAGGCATAATTAATGGTAGACTTTTGCATAGCGTCAGATCAAGGAAAGTTTAGAAAAGAAAACCAAGATAGAGCCATTGTTGTTAATGGAGAGTATTGAACATTGGCTGCTTTGTGTGATGGTATGGGCGGACATGTTGGCGGTAGCCAATGTTCAACATTATCAACTAATAGTATTAAACAATATTTCCTAGACACATTTCCTCAAAAGTTGGAATGTGTTGATAAAAAAAACGTTAGCAAATGATTTAACAATGCAATAAGCTTTATAAAAGAAGCTTTAGTTAATTATGCAAAAGAGTATACTGAATTTGAAGATATGGGGACTACAATGGTTGTTGCCCTCATTTTTAATGCAAATGGTTTAGCTTATGTCTTTAATATTGGCGATTCACGCTTGTATGCATACAATGGATTACTTTATCAAATCACAGAAGATCAAAATTATTTATATCAGTTAATGAGAGAATTTAATTTAACATACGAAGAAGCAGCATTAGATCCTAATTCATACAAACTTATAAGTTGTCTAGGACCAAATAAAAAAACCAATTGTCAATCATTTTTTATATCACAAAAATCAGCAGTTAAATATTATTTATTAACATCCGATGGATTACACGATTATGTTTCTAAACCAATAATAGAAACTGTTTTGCAAACAAATAATAGTTTAAAAGATAAGTTAAACCTTCTAATAAAATATGCCAAAAAAAATCTTTCAAAAGACAATATAACCGGAATTCTTGTGGGGTTGAAAAATGAATAAGATAAAAGAATTACCCGGCATGTTTGTACTTAATAAGTATTTTACTGATTTCGAATTAATTGGGACTGGAGGATTTGGTGAAGTTTATTCTGCTACATATAAAAAAGATAATAAAAGAGTTGCAATTAAAATACTAAATTGTATCGATGATAGCCGTTGGACAGAGCAGAGAACGAGATTCATTAACGAAATAAATATATTAAAGGAAATTCATAGTTCTAACGTAGTAAAAATATTGGGCTGACATTATAAAGGAAACGAAGTTTTTTATGTAATGGAGTTAATTAAAGGCCAAGGTTTAAAAACAATAATTGCAAAAAACAAAAAAATCGATCCTGATACTGCAATTATGTATGCAAAGCAAATATGTCAGGGTTTGATTGAAATCCATGCTGCCAAAATAGTTCATAGAGATTTAAAACCAAGCAATATATTGATTGATGAAGAAACAAACAGCTTAAAATTAATTGATTTTGGTATTTCAATAGGTGAATCAACTCAACGTGTAACTAGAAATTATAAAGCTGTCGGTTCTGTTCAATATATGGCGCCTGAATTATTGCGGGGAATAGCTGATCCTTCTCCAAGAAGTGATATATATGCATTTGGTATAATTTTGTTTGAAATGCTTGAAGGAAAGGTTCCTTTCAATAATCATGATGATATTAACCAAATCATGATGATGCACATCAATAATGAATTGCCTAGAATGAGTGATGTCAATGTTATTATTCCACAAGCATTAGAAAATATTGTAATTCGTTGCACTGCAAAATCTGCAGAGCAAAGATATACTGATTGCATCGAACTAATGCATGATTTAGAGACATGTTTAGATTCAAAAAGAGTAAACGAAAAAAGATTGGAATTAAATCCAAGTAAGTCAAAAATGAACGCCAAAAGAATCTTTAATAGTAAATTATTCAATATTATTTTTTTCACAACAGGAGGCTTTTTAGTAGCCTTTATTGTGATATTCGTGGTTTTATTTTTAAAGGGGATATTGTAGATGATAGAAGGTAAAATAGTTAAATCAATTGCTGGTTTTTATGATATAAAAAGTTTTGAAGATAAACAAATTTATCGTGTAAGAGGAGGTGGCAAACTTCGCTTGCTTGATATAGTGCCAATTGTGGGCGACAACGTTTTGTTTAAGAAAGACGAATTCATCTATCAAATTTTAGGTCGCAAGAATTTTTTTATTAGACCTAAAATTGCAAATGTTGATCAAGCAATTGTTGTGATGTCGTTGGTTGAACCTGAATTTAGTTCATTGCTTGTTGATAAATTTTTGATTATTATTGAAAGTAAGAATGTCAAACCTATTATCGTATTAACAAAAAAAGATCTAACTTCTGATTCAAAAATAGCTTTTTATAAAGATCAAGGCTATGAAGTTTTTGAAATTAATTACGAAACAGGCCAAGGATTTGAAGGCTTAGGAGAAATATTTAAACATAAGACAAGCTTCTTTGTTGGTCAAACAGGTGTAGGAAAAACTACCTTGATAAATTATTTGGCAAAAACAAATTTTGAAACGCAAGCAATTTCTAAAGCCTTAAACCGAGGAAAACATACAACAAGAGAAGTTAGTTTGATTGATTTTAACGGTGGTGAAATAATTGATACACCAGGTTTTTCTTCAATAGAATTTGATTTAACAATTGAAGAACTACCGATTGCTTTTAAATCGTTTAGAGAGGCTGCAAGTAAATGCAAATTTAGAACAAGCTGTAAACATTATTTAGAATCCGTTGAGGATTGTGAAGTTAAAAAATTAGTTTTACAAGGTAAAATTAAACAAGAAAGATATGATAATTACATTAGTTTTTTAAAAAGACTAACAAATCCAATCAAATAGGAGAAATTATGAAAAAAATAAGTCCATCAGTTTTAGATGTTGAAAAGAATAAATTAACAAACTATATTGACCAATTAGTTGATTGAGGTGTTTGCAATGTTCATTATGATGTAATGGATGGCATTTTTGTAAACAATACTGCTTTAACATTTCAAGAAATCAAACAAGTTAAAGAAAATTGCAAGAAACATACAATGGACATTCACTTGATGGTTAAAGATGTTTTCAAATATTATGAAATGTACAAGAATATTGGAGATATATTGACATTTCATTATGAAGCATTTGAAAATAGTGATCTTGAAAAGATGATTAAACAATGCCAAAAAGATAACGTTAAACTTGGCTTAGCAATAAAACCTAATACACAAGTTGAAGAAATAAGACAATATTTAAAACATTGTTCTTTGGTTTTAATAATGAGTGTTGAACCAGGTTTTGGTGGCCAAAAATTTATTGAAAATTCATTTAATAAAATAAAGGAATTAGCAGACATTCGTAAAGCTTTAAATTTAAACTTTATTATTGAAGTTGATGGCGGAGTTAAAGACCAAAATATTGCTAAATGTTTTGAATGTGGTGTTGATTTAGCAGTAGTTGGTTCTTATTTAGTAAAAAACTTTTCAAAAGAAACAATCGAACAACTTTTAAAATAATTATAATGAAAAAAGCAAATAAAATATTACTAGCTACATTTGCAACTACAATTTTAGCATTGCCTCTTGCCACAATTTCTTGCAAAGTCGATCAAGTTAAAGAAAAAGAAAAATTAATAAACAATTCTAGTCTGCTAAATAAAGAAAACAAAAAAATATTATTAAATGAATTAAACATATTAAAAACCCGTTTAGCAAATTCAAAACTTAATGAGAAACAAAAAAACGAATTTATTGAAATTCAATTATTTTTAAAATTAATAACACTTAAAGAACTAAAAATATCTAACGAATTATTAAAATATTATAAATTAATGGCCTTGGTGCAAGATAAGTCTGAAGTAAACAAATACGCAGATTTAATCAAAAAATATTCAAAAAAATTTTCTGATGTTTTGAAGGATAATAGTTCAATATTAAATGATATTTACAATCAAGTTACAAATTTAATTCCTAGAATTAATTTGCTTAAAAATTCTTATATTTTTTCATTAAATATAAATAATAAAAAATCCACTTTTTTGAATAATAATGGAATACTATTCAATTTGGATTTTATATTGAATAACATTGAAAACAAGGAAAACAAATATTCAATTTATTACAATACAAAGAAATATGAACTAAATTTAGAAATAAAAAACATTGAATTTAATTCAAATATTAAAACTGCATTAGATTTTAAAAATCAAGATTTTTTAACAACTAATTTTTTGGATTATGATAAAGATTTAGTGTGCAATGAAATGTTAAAAAATGCAATGGTTTATAAAAATTATGAACTAGTTGAAGATTTTAATGAAAATGATAAGAACATTAAATTTTCAAAAATGTTTACTAGTCAAGATTTTGCAAATAATTATTTGGTTAATTGATTAACAAACGAACCAAATATATGTCAAATCGGTGAAGATAAATTTTTAATTTATTACCATTTACCTTTTTCAATAGAAAATAAAAAAATTGTTCAAAAGCCTTCATTGTATATTTCAAATGTTGTTGCTGATTCAACAATTATTAAAGCAATAGCAAGCAGGAATGTATATTTATTAAAAAACTTATTTTTGTTGTTGCCAAAAGAAAATTATAATGAAAATTCAAAAGTCTTAAATAACTTAATGAAATAAGCATTTGAAAATTAGACTAACAATTAAAATACTGGTCTAATTTAGCTTTGTTAAAAGTTTATAAAAAGTTTGAATTGAAAGTATTAAAAATATCAAATTAAACAAGATAATTATTAAAAAACGCTTATCTTGTTTAATTTGATTTTTAATTTTGTGTATTGAAATAAAAAATCCTAAGCAAAGGCAATGTCAAAATATAGTAAACAAAGTAATATAATTAAAATGTATAAAAAAAGTGCTAAAATTTAAATAAATTTTTGAATAAATTAAAATAATAGATTAATAAAGAATAATTATTTTTTGCTTGCGCCTACACTTTTTAACTAACTTTTTAAATTGATTGTTAAGGTTCATAATTCTGAGCAAATTAGAAAGGAAAAAAATGTATAAGAAAACAAATAGAAAAATATTGATTTCTCTTGCCATATTATGTGGAGGTTTAGCGGTGGCATCAATTGCTACAGTTGCTGCATACGCATGTAAATCAAGATCAGAATCAAAAATAGAAAAAGAAAATCTTTTGAATGAACTTAAAGAGGCATTAAATCAATTAAAACAATCGATTGCATCACCAGAAGCTAAAAATATTGAAAAATCAGTGGAAATCAATATCTTAAATAATGCTAATGTTTCAAAAGAATCCTCAATTAAAGAAATTCAATCTAAAATCAATCAAGTTAAATATGCTATTGATTCATTAAACAAAAAAATCGCAGATCGAAATAACAAGGAATTTGAAAAATTTGAACAAATCAAAAACGATCTTCAAACTTATATTAATAGAGATTTAAATGTTGTTATATACAATGAAATAACTAAAAAATCTAAAAATGAAATATCGAAATTAAATAACATATCGCAAAGCTCAAAAGAACAAGAAATTATAGACGCAATAAGCAAATTGTATGATTTAAAGAAATGAATTTTAGCCCAAAAATTATTAATAGATAATCCAATAAATGAACAAAAAAGACAACTTAAGTCCTTAATTAAAATTGCTAATCAAATATTGTTATCAAACAAAATAGTTGAACGTGACCTAGAATTTAAACAAGAAATTTCTAAGGCTGAGGCATCATTAAGTCAAACAAACAAAACTGCATCTGAACTTTTTAGAGAAAGAATATTGCTTCACAAATTTATTAATGAAACTCTATTATTTGAAGAAACAATGTTTAAAGAAAAGGAAACAAAAATTAACGAGCTTAAGAAACAATCAGAAACAATTTTTGAACATATTGGAAAAGACCCAAATAATAGCATATTCGATTATAATTCCCAATTTTATAGAAACATAATAACTAAAATTGATAGTTTGAACACTAATGAAAAAAGTTTAACCTTAAATAAAAAGATTGAAGATATAAGTAATGAAATAAATAATCTAGACAAATCATTTTCTAAGTTGGTTTCTGATACCCTTGAAGAATTTGAATATGAGGATGATCTTAAAGACCATATTGATGTTGCAAAAGATCCCAAAAATTGAATTAATATTCAAAAATATATCTGATACATTAGGACAATAAGCCAAGTTAAAAAACTAAACTTAAAATCGTTAGTAGATTTTTATAGCAATGATCAATTTGCTAAATTTATTTGCAATACAATTTCAGACTTTTTCGATTATACAAGAGCAAAGAAAGATTTTGATTTTACAAATTCTGTTATTGCAAATTATGTACTTAATAAAATTATTAAGATCAGGGATTTTGAAGAATTTAAAAACTTAAATAGTTATTTTGCAGATAAAAAAAATGATATTTGAGCAATGCAAGTAGACCAAATAACAGACGAAATGAAAACAAAAATCTTTGATTTGTTAATTAAAGATTTATCTAATATTTTAAAGAAAGATAGTTTTGAAAGCAAACTATTAAGTGCAATATTGTTGCCTACAATATTTAAAAAGCCATATGAAAATCTTTCTAAAATAGTAAAGCCAATTATAGATATGATACATAATAAAGACGAATATAGCATAGAGATTATTGGATGAGAAAATGCTTCACAAGAATTAAATCTGGATATATCTAATATAAAAGAAGCAGAAAAAAAGGCTTTAGAAATAAAAGCAAAATTGACACAAGAAAATTTAAAAAATAAATTAAAAGAATTTAAATTAGTAAAAGAAAACTTGGGAAATTTAATTAAAAAAGCATCCGAAAAAGACATCGAAGTCGAAGAAGATACAAAATTCATATATGAAAATTCTGAAGAAGACGAAAATTCTACATTATATGAAGTAATAAGTTCTATTATTAGAATAAAAAAAGAAATAAGATACCTTAAATATAAACTAGATTAATAATCACTAAAATATAATAAAAAAATTCCTTTTGATAGCATTAAACCTGGACAAAAAAATTAGGCTCGTAATGAAAAGAAAACGGGTCTAATTTTTTTGTCCTAGTTTAGTAAAAAGCGCAATTTAATCTTAATAAAAAAATTTGAAATTGAAAAGAAGTTCAAAAGTTAATTTAAAAATTGCAAAATTTTGTGTAAATTTTATTTTTTTTGTTTTTTGAATAATAAACAATTTTTACACTAGTAAAACAAAAAAGCGAGATAAGAATTTATATACTTATCTTGCTTTTTTTATTTTTTAGATATTAAAAAGCAAAAATTAAACCAATTTTTGCTATATGTTTATTATTTTCTAAGAATTTTTTGACCTTTAAAGGTAAATCTTAAATTTATATTTCCTTTACAATATTTATATCAATACCAATATCTTTTCAATTTCAAGGCGTATTGTTCTTATTTCCTTTATAGTAAACTTCTTTTAAAGAAGGATAGAAGATTTGGCCGTTGTAGTAATAATGTCAAACTCCAGCTAAAAACGCATTAGTTCCAATTTTTTTAACTGAATTTGGAATAGTTGCTACTTCTAGTCTTGAGTTTTTAAAAGCATTGCTACCAATTCTTTCAATTCCTTCATTTAAAACTACTTCTTTTAGATTTAAATCCGCAAATGCGCCTTCTTCAATTTTCTTAACAAAATTTGGAATAATTACTTTTTTACGATTTTCAAAGGCTTTTGCTATGCCACTTTTAGCGGCTTTATAGTGCTATTTATATTTAAAAATATTGTTAATTTTTATTTATCATAAAAAATATAAACAATTTTTTTGCTTTATTTTTTATATTTAAAAACATACTTTAGTCAATAAAGATATATGGAAATAAAAAAATAAAAAAATAAAAAATTTTTAGCACTCTTATGCTTTTAGTGCTAAAATATTAAATGTAAATTGAAAAAATTCAAATTAAAAAATATATTTTAAAAGGAGATTAATTATGGCTAAAGAATTTATCTTAGGTATAGACTTAGGTACTACTAACTCGGTAGTATCTGTTATAGAAAATGGAACCCCAAAAATATTAGAAAATCCAAATGGTAAAAGAACTACACCATCTGTTGTAGCTTTTAAAAATGGCGAAACAATTATTGGTGAAAGTGCTAAAAGACAATTGGAATCAAATAAAGATAGTGTTGCATCTATAAAAAGATTAATGGGTACTTCTCAAACAGTACATCTAAATAATAAAGATTACAAACCTGAAGAAATCAGCGCAATGATTTTAAGTTACATGAAAGATTATGCTGACAAAAAATTAGGTCAACCTGTTAAAAAAGCAGTTATTACAGTTCCAGCATATTTTGACAATGCACAACGTGAAGCAACAAAAAACGCAGGAATTATTGCTGGTCTTGATGTTGTAAGAATTATTAACGAACCTACTGCTGCCGCATTGGCATTTGGATTGAATAAAGATAAAAATGAAAATCAAAAGATCTTAGTATTTGACTTAGGTGGTGGTACATTTGACGTTTCGTTGCTTGAAATGGAAAGTGGAACATTTGAAGTTTTAGCAACAGCAGGCGATAACCACTTGGGTGGTGATGACTGAGACCATGAAATCGTTAAATGAATGGTTGAACAAATTAAATCTAAATACAATTTTGATCCAACAACCGACAAAATGGCAATGGCAAGATTAAAAGAAGAAGCAGAACGTGCAAAAATTACTTTATCAGAACAATTAATTGCAAACATTTCTCTTCCATTCTTAGCAATGAATGAAAATGGACCAGTTAATGTTGAATTAGAAATTACTCGTGCTACATTTGAATCAATGACTGAACATTTACTACAAAGAACAAAAAAACCATTATTAGATGTTTTAAGTGAAGCAAAATTAACATGAAATGATATTAATGAAGTGTTACTAGTTGGTGGTTCAACAAGAATGCCAGCTGTTCAAAAACTAGTAGCAGAAGTAACTAATAAAAAACCTAATAATTCAATTAACCCAGATGAAGTAGTTAGCGTTGGAGCTGCTATTCAAGGTGCAATATTAGCAGGAGAAATTCAAGATGTTTTACTACTTGACGTTACTCCTTTAACATTAGGTATTGTAGTAGAAGGTGATGTTGTAGCGCCTTTAATTCCAAGAAATACAACAATTCCTGTTACAAAGAGTCAAATATTCTCAACAGCTGTTGATAATCAAACAGCAGTAACAATCGTTATCACACAAGGTGAAAGACAATTGGCTAGAGATAACAAGATTTTGGGTCAATTCAATCTTGAAGGAATTGAACCTGCGCCAAGAGGAATTCCTCAAATTGAAGTAAGTTTCTCAATTGACGTAAATGGTATTACTAAAGTAACAGCAAAAGATAAAAAGACAAACAAAGAACAAACAATCACCATCCAAAACACCTCTTCATTATCAAAAGAAGAAGTAGAAAAGATGGTTAAGGATGCAGAAGCAAATAGAGAAGCTGATCAAAAGAAACGCCACGAAATTGAAGTTATTGTAAAAGCAGAACAATTAAGCAATGACTTAGAAAAGACATTAAAATCAGAACAAGCTAAAAATCTAGGCGAACCTCAAAAACAAGAACTTCAAAAAGGAATAGATGAAATTAAAGAATTAATAAATAAAAAAGATATCGAACAACTAGAAAAGAAAATTACAGAATTTGAACAAAAAATGGCTCAAGCTGCTGAATTTTTAAAGAAACAACAAGGAAATAATAATCCAAACACCAACAATGACAACCCTCAAACTAACTAAAAATAATTAATTTAAAATAACGGTTTTAAGCCGTTATTTTTTTGTTTTTTGTCTTTTAAAAAAATTCTTCAAAATAAAAAGCAAAAATGGGACTAAATATTGCATTTTTCCCAAAATAACACTTTTTTTATAACTTGATTAATATTTTTTTTGTAAAATTTTTAACATAATTGAAAATTTTAATAGAAGTTAAAGTTTAAAATAAATGGAGGAAAATAAAAATGAAGACAGCTTTAAACAATTATCACAATTTTTTATTTAAAGTTACTGCTAAGAAAAAAAGCACAATAATTATTCCTATAATTTTATTGTTATGCAGTTTAATTTTGTGTTTTGTTTTTGTTGGTACTAAACCAGCACCGCGTTATTTTAATGTAATAATTTTTGCATATACATTAGTTGCAATATTATTTACAGTATTATATGGCTCTCTCAAGTCCTTAAATATATTTAAAGATCTTGAACAAGATGGGATTGAATTAATTATATTTTCTAAGCCCATTTCAAGAAAAGCAATAATCTGAGGGAAAATTCTAAGTTTTAATTCCCTTGGACTTATTTGAACATTGTTTGCTTTTGTTAGTTCTATTATTGTATATAGTCAAGTTTCAAAAGGAAACATGTTTGGATATTTAGTTTTGTTGGCATTGGTTGCACATTTTCTTGCATATACAATTTTTGGATATATAGCAGCATTGATTGCATATAAAGTTAACCAAAAAATTGCTATAACTGTTCCAATCATAATTTTTGCACCTATGGCAATAGGTGGCGGATTTATATTTGCCAACTCAACATCAACAAATGAAAATTTTGCCCATTATATTAATAGCAAATATAAATACCACAGAGCAGGAAATGAAGTTAATAGTGAAGTATTTTATTTAAATAAAAATGATGATAAATATTATTTAGTTCCAAATGGAATTAATAATAACAAGTTTAGTGATGTACAAAATCAATATTTAAATTTAGCTTGAAAATATTCAAATAGTTCGGCTAATGAATGACAAAAATATTCATGACTTGCAATGCCATATCAATTTGTTGATATATTCAACATTGAAAATCAAAATATTTTTAGTAACCTTTCTTCAGACAGTATTAATAATAGTTTAAGTAATTATTTATATTATAAAAATCAAGAAAATACAATATATAAATATAAATTAAATAGAAATTCGGACTTATTAAAATTACCAACATTAGAAAATAAAACTAAGATTAATAAATACTATGTCCCAGGGATGTTAAATTTAAATTCAAATATTGATAATGAAGTAAATAATGGAGTAATTTACGCTAGAAAAAATGCTTCTAATTTTTCTGCTTCTTTTGAAGAAGACAACTTTGTTTATGCATCTCCAAATAATCTAGTAGGAAAATTAAAATGAAAAATAATGAGCCAAGTTTTAAAATATCATAAATTTACAGAAGCAGCCGAAGAATTTTATTCTAAGGTTAAAAATGAAATTAGTTTAAAGAAATTAGTTAAACCACATGATATTAAAAAGTTATTGTTAGAAACGATTTCAAATGAAGTTAATAATACAAATAGTAAGTTCTTTAATATAGATAATGTTAACTTAGCAGTATTCGACCCTCATGGTGTTAGAGATGGAAGATTACAATCTGAAACAGAAAGAAAAATTTATATTGCCACAGCGTTGCTATATTTTATCTATTTTAAAGATAATCAAGGTCAAGTTATTAAATCATTATTGAAATCTGAAAGTGATAAAACAACATATGCACCAAGCCAAATAGAAATCAAGGTAGATGGTTATATCTATGAAATAGGTGGATATGAAAGCTACGAACCAAAACAACAAGTGCAAGAAAATAAAATAACTAAAAATAAACAAGTTGTAGTTAGATATGAATTAATTCCAAGCAATAAAAATTATTTATTCCAAACAGTAGATGATGTATATTCAATTGAAAGAGATAGAAAAATAGTTAATAAACAATTTTATATTTTAATTTGAATAGTAGTGGCAATAATCTTAATGATGGCAAATAGATTTCTATATCTAAGAAAGGATTATAAATAATGGATAATCATATTTTAGAATTAAAAAATGTAGTAAAAATCTATAATAAATCAGATCGTGGAATTCATAATATTTCATTTGCTGTCGAATCTGGAAATTTTCATGCATTTATTGGTGAAAATGGTGCAGGAAAAACAACAACAATTAAAACAATAATTGGTTCTTATATAAATTTTAAAGGTGAAATACTTATTGGTGGAATTACAAACTTAAACCCTAATTCTAAAAAAATTCTTGGTTATGTTCCAGAAAATGCAGTATTTCCTAAGGAACTAACAACATATGAATATTTAAAGGCCCTTGCATTATTATCAGGAATAAATAAAACCGAAGTAAAGGCAAAAATCGAAAGTTATTTACAAAGGTTTGGAATTATTGAATTAAAAAACGAAAAACCTGCTAATTTTTCATCAGGTCAAAAGAAAAAAGTTTTATTAATACAAGCTTTAATTAACGAGCCTAAATTATTGATTTTAGATGAACCAGCTGCTAATTTAGACCCTACTGCAAGATTTGAATTGTTTTCAATACTACATGAACTAAATGAAAAAGGAACAACAATTTTTATTAGTTCCCACGTTCTAAGTGAAATTGATAAATATGCAAATTCGTTGACATTAATTCATGGCGGAAAGATTTTATATTCTGGCCCTAAATATGATTCATTGGAAAAAATATTTTATGAAAAAGTTATTAAAAATTAGTTTGTCATTGCCATTAGTTATTGCAACAATTCCATTAGTTGCTTCAAGCTGCAAACAAACAAATATCGAAATTAAAAAACCAGATGATAAAAAAGAAACAGCAAATCAAATTCAAGCAAAAATCAATAAAGAATTTCAAAAAAATAAAGTGGTTCAAAAATTACTAAGTTCATTTATTGAAAATGAAAAAGAACAAGACATTTTTGTACAAAATCAAAATAATATATTGCCCAATGTATATTCAGAACTAAAGTTTTCTTTGTTGTATTTTCCTATTTATAATAGAGCCTCAGATAATCCGGGTAGCGAACTTTATGGATTTATAAGACAATCAAGAGAAATTATTGAAAACACATTGCAAAGAAATTGATATTGATATTTAAAACAAATTGGTAATTTTGTATATTCAATAAACCCTTATAATGATAGATACATTGAAACCCCTCAAACAAAAAAAACGATTCAATCTGCCCAAAAAGACATTGGTATTTTATATAAAATAAAAGATGCAATGCCTAAGGATATTATTGAATTGCCTTATAACCCAATAAGTTCAGATGAATTAAAAGAAATAGTCGCTAAAAAAATTAATGAAAGTATTGATTCTAAAGTTCAAAACCAATTTAGCGATTATTTAGAAGATAGAATTGATGAATCATCGAAGTTAAAAATAGATACAAATTCAGAAATAACCAATTTTTTAAGAAACAAAATTAACTTAAATGATGCAAAAAAGCTAATAGAAAATTATTTGAATAATAAGAACAAATTTGAAATTAGTAATCAATATCAAATTAATAATGAAATTTCTAATAAAATATTTAATCTATTAAATACAAAAGGATCTTCATTTGACAACTATACTTCAAAGAAAATCTTTTACGTAGCATATGACTACAAAACTTGAATAAGAGTAATTGTTTATACTGAAAATAACGTTAAAAAAATTTCTATAAATCCCGATGTTTATATTTTAAATCAAAGCAATAGCTATGAACAAAGCAAAGAAATTTTAAAAAAATTAAATAAACAAATTTTTATTGAACGGTTGAATTCAATTATTGATTCTTTAATTGAAAATATAAATGAAAAAGATTTCAATATTAAAAACTTTTTTGAAAAAAATAATGATAAAAAATTCTTTGAATTGTTTCAAAAGTTTTCATACAATAAATACTTGATTGAAGCAATGCAAAAAATTAATAAAGAAAAAGATCTAATTTATCGCTATAGTTGGGAGACAGTAGATTATGAATAAAAGAAATTTATTAAATTTTTTGTTTGTTCCCACTTTAATTCTTGCACCATTAACCGCTATATCGTGCGTTGATAAAAGTGAAAAAAGCGATTTGAATGCATTAGCAACTAAAATAGGGGATGTTGTAAACAAAAAAGAAAATCCTGCTATTGAAGTAACCAAAAAAATAATTAACGACTTATTGAAACAAGTATATAGGGATAATGATGTTGAAAAAATTAATTTTTTGGATCAACAACAAAATAAAGAATATCAAAAAAACATTTTTTTGAAAATTAAAGAACTTTCAAAAAAATGAAATTCTAAATTAAACAATACAGAAAAAGAAAACATTGCCAATCAATTAAATCAAATTTATGAAAAAAATTGATATATTGTGTTAACAAATATAAATAAATTTAATTTAAGATTCTATGAATGACTTTTGTTTGATAAGCCTGAATTTTTAAGTCAAATTAGGGCAGATGGAAAGCATAGCAAAGAATATTTAAATTCATTAAAAAGCGAAAAGAAACCAGATAATGTTCCGATTATTAATAATTATTTAAAGGAATTAAAAGAAGGCGATGAATCAGGGGAACTTTCAAATATGAACATGCTTTATTTAAAAAAAGATAGTATGATATTCAGATTTAGAGTTGTTGATACATTGACAAAACCAAAAATCAAACTAACTTGTTTTGTTTGATATTTTAGTAAATTTAAAAATATATCAATAAATTTGATTTCAGATATTGTTCATAGCGCTTATATTCACGGTGTTCAAGAAGGATTCAATAGATTTGAAAATGATATGGCAAACCGTTATGGCAAACCTGCCAAAATGATCATGGGAATTTTCGATGATAATGCTTCTAGCGCCAACGGAGGGGAAGAAAATGAATAAAAAAATTTTGATGACTCTATCATTAAGCACGTTAAGCATTATACCTAGCTGTTTTTCTTTAATTTCATGTACTAAAGAAAATAAACAAGATGAAAAAAAATCATTGAATCAAATAATCGAGGGCAATGAAAATGCAAACGAAAGAGAATGAAAAAATTTTTTAAATAGAGATTATGTTAAAGAAATTTTAAGACTTTCGTTTGATGCTGATGTTGATGCTAAAAACAAATATATAGCCGAACAAAAAAAATTAAATAATAATATAGAATATTCAAAAAAATTAAAAGAAGCATTGATATATGGAAATTCTTTGAAGGCTTCTACAAGTTACAATAGCGATGATTTTTCATCACAACCTAAATTATTATTTCAAAATGGAAAAAGGATTTTTAATGAACTTAAAAGTAAGAATTGACTTTGATTTCTTTACAACCTTCCAAAAATAATTTTTGGTTATTTTCCTCCAATGAATAAATTTGAATCATCAGATGAACAATCAGCGTTAACCGCGCAAGGTAATTCCTTGATTTTAGGCCATTTTTATAAACCAAAGAGTAATGAAATTATTCAATATACAACTCAAGTTACAAAAAATGACAAAAATGGTGAGAGAACTTATGATGTTTATTTATTAACAAAAGAAGGTTTTATTCTTCAATTAACTATTGATTGAGCAAAAGATGCTGAAGATAATAAACTAAAGCCTAGTGTTAAACTTTTTGGTTATATTTACACCTACCCAAACATTGTAAAAGATAGAAATAATTTAAATATTTTCAATATTCAAAAATATGTTATTGCCAATACAGAATCATTTGATGATATTAACAATAGAACATTGCAAATATTATTTCAAGATCAATATGGGGGCAGTCCCTTACGTTATACAATAATTGATGTTAGTTTAAAATAAAATATATTGTCAAGATAAAAATTCAAGTGTACACAAATTGAAACAAAATTAAGTGTTGCCTTGAATTTTTTTAAATTAAAATTTCAATAGATATGAGCAATAAATCAACATATATCTCATTTTCTTTTGTTTTAAAAATAAAGTTCTTTTGCATATTGTAAATTGTAAATTTTAGAATTTAAACAATTAATTTTCATATTTTTGATACAATTTTATTGTATTTATTACAATCGGTAAATTATTCAATTATATTAATTTATCAAACACTATTACGGATTCATTTAACTAGTGTTAGTAAAGATTACTAATGTTAAATGTTAGAAATAATAGGAAGAACAACAAAAAAAACAAAGGAAAATTATGTCAGAACAAAAACAACAAGCACAACCAGCAGTAGCTCCAACACAAGCAGAACCTGCTATTGTATCACGCGAAAAATTATTAGAAGCAGGAACATACTTTGGACACAGAGTAAGCCAATGAAATCCAAAAATGAAACAATACATTTGAGGAAAAAGAATGGGAATTCATATTATTGATATTGCCAAAACTCAAAAAACATTGGAATATGCTTACAAACTATTAAATAAAATGGCTCAAAAACCAATTTCATTTATTTGAGTAGGAACTAAAAAACAAGCTAAAAAAGCTATAGAATCAGCCGCAGCAAGAACAAATTCAGTTTATGTTTCAGAACGTTGATTAGGTGGAACATTAACTAATTCACAAACAATTTTTAGAAGTGTTAGAGAATTAGAAAGATTAGAAGAACTTCAAAAATCAGAATATCAAGGATATACTAAAAAAGAAGGTCTTTTAATGGACAAGAAAATTGCCAAGTTGCAAAAGAACTTAGGCGGTATTAGAAAATTAGCTGGAAAAGCAGCAATGCCACAAGTAATGATTTGTGCATCACCTATTGATGATGAAATAGCAATTAGAGAAGCAAAAAAGAAAGGTTTGAAAATTTTTGCAATTCAAGATACAAACTCAAACCCAGATTTAGTTGACTTCGTAATACCGGCAAACGACGATTCTGTAAAGTCAATTACTTTAATTACAACTATTTTGGCAGACGCAATAGCATCAGCACATGGCGAAAAACCAGTATATGCATATAAGTCAAATGAAGAAATTATCTTGCCAGAAGAACAAAAACCAGAAAAGGTTGAAAAAATAGCAAACACTGAAGAAAGAAAACCAAGAACTTTTGTTAAAAAAGCTAACGTTGAATTAGAAAATACTAAGAAATCTGAATAAAATAATTGAAAGGGAATGTTATGTCAGTAGATTTAAAAAAAATTAAGGAATTAAGAGAAAGAACAAATTCGGGATTTTTAGATGTTAAAAATGCATTAGAAGCAACAAACAACGATATTGAAAAAGCAATTGAATGATTGCAAGAAAAAGGTATAGTTAAGGCTGCTAAAAAAGCTGGAAGAATTGCAGCAGAAGGAATAGTAAGAGCTGTTATTAAAGATAATATTGCTGTGATTTTTGAATTAAATTCAGAAACAGATTTTGTTGCTAAAAATCAATTATTTTTAGACTTTGCTAAATTATTAGAAGAAGAATTGCCAAAAGTTAATTTCTCAAATATCGATGATTTATTAGAACAAAAAATTAATGGTAAAACAATTGAAGAATATACAACAGATTTAACAGCAAAAATTGGCGAAAAGATTCATTTAAGAAGAGCATTAAAGTATGAAGCAAAAGAAGATGAAGTTGTTGCTGGATATACACATGCTAACAATAGAATTGCTACTATTTTAATTGCTAAAGGACATAACCAAGAAGCATTAAGACATGTTGCAATGCATATTGCAGCTTTAAACCCTGCTCATTTATTCGAAACATGTTTGGATGAAGAAGAATTAAAAGAAATTTACGAACGTATTAACAACGATCCTAAATTAGCATCTAAACCAGAAAAAATTCAAGTTTCTATGAAATCAGGAATGTTAAGAAAAGAATTCAATGAACGTGGCGTTTTAATGTATCAACCATTTGTTATGGAAGAATCAAAAACAGTTCAACAATATTTAACAGAAAATAAATTGCAATTAATAGATGCAACAAGATTTGAAGTTGGCGAAGGAATAGAAAAGAAAACTGTTGATTTTGCTACAGAAGTTGCTGAACAAATGTCACTTAAATAATAATTAAGTGTTAAAAAAATAGGCTTTTTGCCTATTTTTTTTGATACTTTTTAGTTATTTTTTTTAGCTTAATTAAGAAATGATTCAATGCAGAACGAGACTTTTTAATGTTTTTTGTTTCTAATAATAAACTAAGTTCATTCAAGGAACTGTCTAAACTATTTAATTTTAATTCAAATAGTGTAAGTTCTTCAACGCTAAACTCATCCAAGAGATTGTTTTCTTTTATAAATTGATAATTTTTAATAAATTCTAAATTTGCTTTTGCAATACGTTCTTGATTATATACGTCGAGATTTGTTATTCTGTTTGCCGAATTATAAAAATCTCTTTGAATTTTGCCATCTTCGAATAAATAATAAGATTCAATGGTTTCAATAGCTTTTAAAAAATCACAAATATTTTCAATTTTTTTTAAATAAATTATTATTTTTTGTTTTCTAATTATTTTTTTAAATTCTAATCCGTGAGAATTTAAAATTGATAAAGCATTTTCTGCATTTATTAAATCATTAAATTTTAACTCTAAATGATTTGTTCTTCCATTTATTATTGAACCCGAAGAAAGGAATAAACCAGCAAAGAATTTTCCTTGATATTTAACACTTATGTTAGCTAAGAAATTTGTATTTATTGAAATTTCATTTTTTTTAGTATATGTATAAGAAATTTTAAGTTCATTACAAATATTTAATATATATGTCCTAATCTTAATATTATTTATTACTAATTTAGCTTCATTGTTTTTTACATTGCAACAATAAATTATGCCAGCCATCAAAAAAAACTTTTCTTGTTTTTTTAACTGAAAAGACATAATTTCTTCTTTTACTAGTTGACTAAAAGATTTATTCTCCATAAATGATTTCTTTCCCTCAATACATTAAATTATATTATATAGGAGTCTTTATTTAATTTTTATACCAGCATTAGCCATTAAATTTAAAGCAAAATCGTGATATTCTTGAGAATTATGATTTGGAATATTAAATGTTGCAATTAAATTTTTAAAGACAACAATTTCTTTATTTTGCTTTAAATGATTGAAATAAGTTTTTAGAGTTATTGCAAATTGCAAAATGCAAATATCTGAACAACAACCAATAATTTCAAAGGAATCATACTTGCTTAAAATTTCCTTTTTTAATGCAAAAAAGGAGTTAGTAGAATTCTTTTCAATTATATTTTTAGCATATTTTTTTAATTCATCTACTACTTGACTTTCTTTGCTATTTTTTAAACAATGTATTGGGTATGATTGCATTTCAATATCATCGCTACTATGAGCATCGTTTAAAAAAAATATATCATTGTCTTTATATTGAGTTTCTAAAAAATTTTTTATAGGAACAATTATTGACTCAATATTGCAACTATATAGTGCACCTTTTTTACAAAATCCATTAACCATATCAACAACAAAGATTGCTTTCATATTTTTTACTCCTTTTTTTCATTTTTCAATGTTTTTGTATTGTCATATAAATTGTTAATTATTGTATAATTATAGCGGATACATCTTAGAGGGACATTTATTTTTAATTATATGTTGGTGTATCTAAAGTTCGAATTTTATTTATTTTAATATATAAGAAAGGATTTCTAATGGCCGAAGAACAATTAGTTGTTACTTCAAGTCCTAAGAAAAAGATTGGCTTCTTTTCAGCTATTCTTATCGTTATGGGTTCGGCTATCGGTGTTGGGGTTTTCCTACGTTCAAAGAACGTTTTACAAAACTCAGCTAACAACGTCGTATGAGCATTGATCGTTTGATTAATTGCTGGATTTGCCGTAATTACCATGGGTATTGCATTAGTTGAAGTTGCATCAGGACGTAACGACAACTTAGGTGTTATAGGTTGAACCAAGGCATTTAGTACATTAAACTTATACAAAGGTACTAAATTCTTTATGACCTATCTATATCTACCATTTACTTACTTCTTTATGCCTTACTACGTTATCGTTCAATTCCAAGACGGTATCGCAGGATTCATAGGATACAGCAAAGTAGGATTTGGTGGTTCTACAAGTGCTCCATGAATCTACTTTGCAATAGGTCTAGCACTTACATTATGAATGATTTTTTCAGCCGGTATTAGTGGCCGTGCTACAAACATTCAAAACTGAATAGTTACAGTTGCAAAATTAATTCCTATTTTCGTAGTTATTATTTTGGGTATTATTTTTGCTGTATACAAAGGTACAGGAAAAGAAGGATTCGTTGCTGGAGTTGATGATGGTGTTACACCACTATGACACAAAATAACATCAGCTGACCTATTCAACAAGAGTTCATCATCATTCTTAGGATTAACACCATTCCTAACTGTATTTAGCTCACTAGGTGGTATCTTCTTTGCATTCGACGGTTTCTATGTAACCGCTGGTATTCAATCAGAAATGAAACACCCAGAAAAAACCCCTGCTGCTATTGTTATTGGGTTAGCATCAATGACTGTTCTATACATGTTAATTGCTGTTACAATGACATTAGGTGGAAACAACGGTTCATTCTACGGATTCGCAGATGAATTAAACGCTGTTAAAGCAGGTTGAGTATTTGGTGTAATTAACATTTTAATCTCAATCGGTATTATTGGTATTTTAAACGGTTTCACCGCTTGAGCTACAAGATGAGTAGAAGACTTAGTTAGAGAAGGTGAAATGTTCATGCCTGTAAAAGCTTACAGATACATTAAACATTCACAAGTTCCATGAGTTGGAGCAATCTTCGTATTATTAATTTCTCTACCATTCATGGTTATCTTTACTGCTATTGGTGCTTACGCATACTTTGGTGGAGGATATTCAACAGACTACGGTTACCAAATTGATAACCTATTAACATTTGCTGACTTGATGGCAAACTGAATGGCTGTATTCGCATTCGCATTCATTGCAACATCAATTATCGGAATGTTACAAAACAGAAAGAAACACTTTATTGCTGTTGTTGAAGGAAAACACAGTAAATGAACAGGATACGTATCAGTTATCTTCATCTTATCAGTTATGTTCTTCTTAGCAATTGACCCATTCATATCAATAGGTCTATCAGCAGCACAATACGTTGCAAATAAAACAGAAGACACTAGAAACGGCTTAGTAGGAAACATTGCTACATCAGTTCTATTTATAATATATGCAGTAGTTTCATTCTTAGGCGCTCCAGTTGAAAGACAAATTGCTAAATCTAACAAGATTAAATTTGATAGAGTTCTTTCAGGAAACATTTGTGCACACGAAGTTCCAGAATGATGCCCATGCAAATTAGAAGAATTAAAAGAAAAAGCTGAACTAAATGACATGGTTCTAAAAACATTTGCTGAAGCAAGATAATAATTTGTTATTTCACCAGTGGGTTATTCCCACTGGTTTTTATTTATATTTTTAATATTCAATTAAAAACTTAGTTTTAATGTTAAAATGTAAAGATAATTATTTTATAAAGGTAACCAATGAAAAATAGTAAAGAACAGATTAACAATATTCCTTATGTTTTAGATGAAACATTGATTAACACAAATTTAAAAACTGGCCTAAGTTCACAAGAGGCTGATTTAAGACTTAAAAAGTTTGGAAAAAATGAATTGCATAAAGCAAAAAAAATAAATCCATTTTTAATATATTTAGAGCAATTTAAAGATATATTGGTCATTATATTATTATTTGCAGCAATAATATCTTATGCTCTTGCAATTGTTTCAGGAACTCAAAATAATTGAAATTGATCTATTAATGGTAATCGCTTATTAATTGAATTTATTGAACCTACAATTATATTATTTGTAGTTCTTACTAATTCTCTAGTAGGCGCTATTCAAGAAATTAAGAGCATACAAGCAGTTGAGGCATTGAGCAAATTAAGTCCCTTACAAGCTAAAGTCGTCCGTGATGGCAATTTAATAACATTAGCATCAAGTGATATAACCGTTGGCGATATTGTTTTAATTGAATCAGGTGATGTTGTACCTTCTGATGGCTATTTGTTGTTTAGTTCAAATTTAACTGTTATCGAGAGTGCATTGACCGGAGAAAGTGAACCGGCGACAAAAGATTACCAAGCTAAAAGAAATTTAGCATTAGCAATGGCAGATAGACAATTTATGGTTTATTCTTCAACTGTAGTTGCTACAGGAACTGCTTATTTTGTTGTTACATCAGTAGGTGAAAATAACGAAATCGGAAAAATTTCTAAATTAGTTAATCAACAAAAAGAATCAATGACTCCTCTACAAATTAAAATCAATAAATTAGGAAAAATATTTGGATATTCAGGCCTTGCATTATTTTTAGCAAGTTTAGTGACTCAAATAGTTTTCCATGCGGTATCAAGAAATTCCTTTGCTGATACAATGTTTTGAGGCAATGCAATAGTTGGAGCCATTTCACTAGCCGTTGCTGCAATTCCTGAGGGGTTAATTGCTTTTACTTCAATTATTTTAGCCATTGGTGTTCAAAGAATGGCAAGAAAGAATGCAATTGTTAAGAACTTAATGGCTGTTGAGTCTCTTGGTAATTGTGCAGTAATATGTTCAGATAAAACAGGAACATTAACACAAAATAAGATGACAATTGTAGATGCTTTTGATGGAAGCCAACTACTATCAAATAATAGTGAAATTAAAAACTTCTTAAATATTATAGAATTAGGTTCATTGTGTTGCGATGCAAATTTAACTCGCAATGAAAAAGATGAATATGTTGTAGTTGGAGATCCAACAGAAATAGCATTTTTATATGAACTTGAAAAATTTTCTAATTTTAAATACAAAAACAACCTAGAAATAGAATATAAGCGTTTGCATTTGTTCCCATTTGATTCAGATAGAAAAATGAAATCAGTAATAAATCAAAACGACAACAATTATTGAGCAACGGTTATGGGTGCTCCTGAAATAATAATTGAAAAATGTAATTTTGATTCGCCTTCATTAAAAGAAAAATATTTACAAATAAATAATCAATGGGCAAATAATGCTTATAGAGTCTTAGCAATAGCAAGAAAATCCTTAAAAAAGGAAACAATTGAGAAATTTAATGAATTGTCAATTTCTAAATTACAAGAGCATGTAGAAAATGATTTAGAAATTGTTGGATTAGTTGCAATGATTGATCCTCCAAGAGAAAGTGCAAAAGATGCTATTACATTATGCAAACAAGCAGGAATAAAACCTGTTATGATTACTGGTGATAATTTAAATACAGCAAAAGCAATAGCAACAAATCTTGGTATATATAGTGATCAAGATCGTGCTATAACAGGAACTGAACTTTCAAACATTTCTGATGAAGAATTAGTTCAAAATATTGAAAAATACAGTGTTTATGCTAGAGTTAAACCAGAAGATAAATTAAGAATTGTTAATGCTTGACAACAAAGAAAGCAAGTTGTAGCAATGACCGGTGATGGCGTTAATGATGCTCCAGCATTAAAAGCTTCAGATATAGGCTGCGCTATGGGTATAACAGGAACTGAAGCTTCTAAGCAGGCTGCAAATGTTATATTGGCAGATGATAATTTTGCCACCATAGTTTCAGCAGTAGCAAACGGAAGATCAATTTATCAAAAAATTAAAAACGTAATTCAAAGTTTATTGATTACATCAATTGCAGAGATCATTTTATTATTTATAGGTTTATTTTTGTTTAAAGCGATTTTTTCAAGTGCTGAATGAAAAGTAGCATTAGGAAAAAGTGATTTATATATAATGTCAGCTACTCAATTATTGTGAATCAATTTATTTACTCATGGTTTTCCAGCAATAGCATTAGGGCTTCAAGATTCTAAAGAAGACTACATGAATAGAAGGCCTATATCAAAATATGAGTCAATATTTGCAAATCAAATGGGATTCAACACTTTATGAAAAGGAATTTTGATAGGAATACTATCGCTAATAACTTATTATTTAGCAGCGTTATATGGCAAAAATAATGGATTTAACGCAGAAGATATATTAAAAGCAGCATCTACTTGTTCATTTTTGGTTTTAGGTATATCTGCTACATTCAATTCTATAAACTTAATGACAAAGAAATCAATCTTTATATCTAATCCAATATATTATTGAAAAGTTTATGCCTCAGTAATTTTTTCATTAGCATTTCTATTGATAGTAGTATTTGTAAAAAATATAAATCTAGTGTTTGGATGCTACACCGAAATTCATTCTAAGCCCGAACTTTTAGCACTAGGATTAGCATTACCATTAGTAATTATTCCAATATATTCAATTCATAAATTAATCATATATTTAATCGAAAAAAACAAAATTAAAGAAAATAAAATTACAGAATTTAAAATGATATTACCTCCAAAGCAAGTCAAAAGAAATCAAAAAAATGTAGATCTATAGAAATCTACATTTTTTATATTGCAATATAAATTATGCAATTTATTGTTACTTGAGTAATAAGACTAATGGCTTACACTCATTACAGTAACAAATACAACAAAGGCAAAAAACATAGTTAAGCAAGAAATAATAATTCCTGCTATTGACAAAGTTCTTCCTGAATCATTCATTGATTTTGATTTGCTAAGTCCAATACAGCTAAAAATAAGACCCAACATCGCAAAGAAAAACGACAATATAAATCCGGCAATAGCAAATCCATTAGTTTCTGAACTTTGGATTTGTTGTTGAGGTTGCAAATAAGGCTGAGACGGTTGCGTTGTATTCATTTGTGTTGTTACATATGCATCTTTTTCCACCTTTTTTCCGCAATTTGGGCAATAATTAGCATTATTTAATTTAGTGCCACAATTTGGGCAATAATTCATACTATCTCCCTCTTATTAAAATGCATAAAAATTATTTTTTAAATTAATTTTATATATAAATATAACACAATAAAGCAATAAAAAACATCCCAATTCTTGTTGGGACGTTTTTTATTTAATTGATTCTTTTTTTGTTTATATATATTTTATTTTCTATATCAAATGTTTCGCCGTCAAGTTGCATAAAGTGCATTTCCTTACATTCCATGCTAAATTTATATCCTTGAATTTCATGTACATATTTTTTATAACGTAAATGTTTTCCACTATATACTGAAAAGAATAATCATGCTAATTTTCATTTAGAGACGTTATTAATAATGATTAAATTTAATTTGTCTGTATTGTTACAATTGGGAGCTAATTTCATTCCACCACCAAAATAACAATTATTCATAATTGAAGCTAATCAAACATTTTTAAATTCTAATTCTTTTCCATCTACATTAATTTTTATTAAAGGAATTGTTTTGAATGTCTTAAATGCATTTATTGTTACTTTAGCAAATGATGATTTATTTTTAAGCATTTTTTCATCATTAACTTTTTTTGCAATAACTGCATCAATGCCAAAACCAATGCCATTTAAAAAAGATCTTTCATCTTGGTCGTTTTTAAATTTAATTGTTGGTAGGTTAGCAATGTAAGGAGATATCAAATAATATTTTTTATGTATTATTTGAACATCTTGTTTTATTTTTAAAGACCTTAGAAAATCATTGCCCGTTCCTGCTTTATATGCATAAATTTTGGGTAAAGGTTTTATATTGTGAATTTTATTAGTGATATTGGTTAATGTTCCATCACCGCCAATTAATAAAACAATATCTTTTTCTATATCCATTTGAGAAACTAGATTTTGACTGTCTTTAATTTTTATAATGTCAAATATTTTTACCTCAGGTTGTAAAAATACTTTAATGGCGTCAGAAACTATGTCATACATCTTTCTTTGACATTTTCCACTCTTTGATAGAGAATTATAAAATATATATAGCATAAATTATTTTTCCAAATCTTGTAATCTATTTAATGCATTGATATAAATTTCAAGTGATGATTTTATTTCATCAAATGTAAAATATTCGTTTGGTCCATGCATTAAATGCATATATTTAGTTGATCCAAAAGCGACACAAGTTTTTATTAATCTTGCAAATGTACCGCCACCAATTGCTAAAGGTTTTTCATTTGAATTCATCGCTTCATTATATGTATCCATAAGAATTCTTACAATTGGAGAATCTTGTTCAATATACTTTGGTTCTTTGAATCCTTGCAATTTTGAAAATACATCATCGCCAAATTCATCTTTTAAATAATCATTTAAGCTTTTGGTTACATCATCAATTTTATGAGAAACTGGGACTCTAAAATCAAAAGTTAAAACATATTCATTTTTCAAGGTTCTAATAATGCCTAAATTTGCAGATAATTCGCCCGAAAAATCACTATAGTTTTTAAAAATATTACTTAAATTAAATGATTTGCTATTTAAATTTTCTTTAATAAATTTAAATAATTTAGATTTAGCAAATTCTTTATTTTCTTTGCTTGCTATATTAATGGCTTTAATTATCGCATTGTCACCTTTTTCAGGCGTTGAGCCGTGTCCACCAATTCCCTTAATAATATTAGGTTTTTCATTCAATACTAAAGATGTTGAATCAGGAATTTGGTTAACAACGTCGCCCCCATTTAATTCAACTCCTGGAACCTTTGGAAAATGAATTTCTGTTACATAGATCATTTTTTCAGCATATATTAAAGGTCATTCACCATCAGGAGTATAAGAAATATAAGGATCTTTGAAGTCAGCCAAATAAGACTTCATTGATTTCATTGTGGTTTCTTCTGAAATTCCAAATATTATTCTTATTTTTCATTCGTTAGATAATAAGCCATGATCATGAATGTATTTCATTGCATATAAATTAATAATAGCAGGTCCCTTATCATCTAAACTGCCACGACCAATAATTGATTCATTTGTTATAACTGGGACAAATGCACTTGTACGTCATTGGCTTTCATCTCCTGCAGGAACAACGTCAAGGTGGGCTAGTATTCCAATAATCTTATCTCCATTGCCAAATTCAGCAAACCCATAACGATTTTTAGGATCTTTATAAATATTAAATCCAAAACTTTTGGCAAGATTTAATGCATAATTTAAAGCATTGTCAGTTTCTTTTCCAAAAGGAAATTCACTACTAAAATCTTCTTCACTAATCGAAGGAATTGCACAAATATTTGCAATATTTTTAATCATTTCATCAAAGTCTTCTTTACTTTGCTTATAAGTAATAAATTTTTTCATTCTTACCTCTTATTAATTTTTTTAATATATTATACATTTTAATCCAAATAAAAAAAATAAATAAGGCGTAGCCTTATTATTTGTTTAATCCAACAGCAATACCTTTGTTTTTAAAAACGTAGTCATTATTATTGTTATATACAACATCAATTAATTCGGGATAATCAATAAAAGGATTTCTATTTCCTTGATGTTTATATACTCCATTGTTTCTATCAATGTCAAATTTTGAAACCTTATCTAAATTATTTCAAGCTAAAAATGTTTTTAAAAATATAGGAGTTATAGTATTTTTTCCATTATTTTCTTTAAATACACGAGTTGATTGATTATTTGATGTTATATTTAAATCGTTATAAGTTAAACAAAAGTATAAATATGCCCTAGCAACATCACCCTTAAATTCATTAATAGGTTCAGTAACAGATCCACCATCTTCAACTGACAAACCAGTTTTTGTACCGTTTTTGGATATTTTGTTGCCTTGCAATTCCTTAACTGTTCCATAAGGGTAATTTGCATGCCATGCATTAACTAATTTATCGGTTGGTCAAACATGGTGAGCATCTGTTCTCATTGGAGCATTTTTTCTAAATCATGATTGTGGAACTAAGTGTTCTCTATTCATTCCTTGGCCTTCACGCTTGCCTGTATCTCTATATCTTCCATGTCAATTAGTAAAAACATCTTCTTTATCTGGGATTTCTTCATAGATATCTAAAACAGATCCATCTTTTTCATAATATTTATCAACAAAAGCATCTTTATATGTATCAAATAAGTCATTATATTTTGTTGAGTGATTTCTATGAGCTTTTTGCAATGCAAATAAGTTATCTCTTAGGTTTTGTCCACTTAAGCCATCTAAGGATTTATAAAAGTCGTTTGAATTATCGTAAATTATCTTATTATCTAAGGATTTAGCTTCAGGGCTCTTTTCTGGTGTTGTTTTTGGTTTTTCACCTTCTGGTTTAACTTCTGGTTTCTTTTCTGGTGTTGTTTTTGGTTTTTCACCTTCTGGTTTAACTTCAGGTTTTACACATCCAGCAGCAACTAATGGCGCTGAAATTAGCAAAGGTAAAATAGCACCCATAGTTAGGAATATTTTTTTGGATTTTTTCATTATTTTATATATTTCGCTTTCTTTTTTGTATTTAATAAAAATCTATCATATGAATTAAATAAGTCAATTCAAATTAATAATAATTTTCAAAAAAGCAAATAAAAAAACACCTATAAGTGTTTTTTAATGTTTAACAAAGAGTTTGCAAAGAAAAACTTATTCAGCTTGTTGTTTAGCAAGTCTACGGCGAGCTCTGGCTTTTTCTAAAGCTAATTTATGTTCTCTTTTAGCTGCTTTTACCTTGTTAGCTAATCTAACGTCTTTTCTCATAATTTCTCCTTTGCTTTGTTCAAGCAATAATTATATCTATTTTTTCTTAATTTGTTTAATTTTTTGCATTATTGTTTCAAGATCTATTTTTTCAATTTCTTGTATTTTCTGACTAACGCTTTCTTTTGTTGCTATATTTTTTTTATCTTTTTGGTTGTCATTAACATTATTTGAAACATTTGGTAATTCAATATCTTCAGTAGATATTTGAAATAGTTCTTCTTGTTCTTCATTATTTAAATGTTGAATATCTTCATTATAGGATTTTTTAATCAAAGCATGTTCAGCAGTTAATTCAATATTTTTATCAATATATCCTTGATATTCTTGTCTTATTTTTTCCTGCTCTATTTCATTTGCTTCAATTAATTCATTAATTTTTATTGGCGTAATAAAATACCCAGGGTTAGTAAATGAATAATCTAATTTAAGAAGTTTACTTTCCTTAGTCTTATCTTTTAATATAAATTTTCAATCATATTCTGCCAAATTACCATTATTATTAGTGTATAACATTTTTAAATTGCTCAATGTAACAGCAAGTGATTGAAGACCTAAAATATTATTTTTTAGTGGCGTAAACAACATTTTTGGAGTATTGCGTGTGGTCAATTCTAATTTGCTAGTATCCCAACATTTTATATAGCCACCTTGCGTTAAATAAATTATATTGTTGTATTTTGAAACCGTTATAAAACCACTTACTTGAAGTTGAGGATGTTTATATAAGTTTAAAAGAAGTCTAATGCCTCTTGCTTTCAAAGAAATTCCAACCTTTAGTTCGTTTTCTGAAACTAAATGATACATACCATTATTTAGTAAAATAAATAGCATTTTTTCATCATTAGAAATTTGGGCATCTATTAATTCATCATTATCATCCTTAAACGACATGCAAGTTTTTGTTTTAAGAGAAGATTTATTTTCAAAATCTCTTAAATTAACTTTTTTGGCATATCCTAATTTTGACATTAAAATAAAGTTTGCATAACTATTGAATGACGTAATTTCTATAACTCTAATAATTTTTTCTGAGCTTTCTAAAGCTACTATTGATGAAATATGTTGGCCAAGATCTTTTCAATTGCAATCTTTAAACAAATGGCAATCAATAATAAAATAATTCCCTAAATTTGTAAAGAATAATATCTTTGATAACGAGTTTACTTTATCAAAATAGAAAACATTATCTTCTTCTTTTAATTTGAATGTGTTTAATTCATTGCTAGTGTAAACTTTTAAACTTATTTTTTTATAATATCCATCTTTGGTTATATATAAATAAAAATCTTCATTTTTTGTCAATAATTTATGATCAATTTCTTTATCGATTTTTTGATCTGAAATTTCTGTTAATCTTGGCTTTGAATATTGATTTTTTATTTCAAGCAATTGCTTTATTAAATATTGATTAAATTCTCATTTATCATTTAATAATTTATTGCAATTTTCAATTTGAATTTCAAGGTTTTTCTTTTCTTCTTGAAATTCGATTTGATCCATTCTTGAAAGCTTATACAATCTTAATTCAGCAATCGCTGTTGCTTGCAATTCACTAAATTTAAATTTATTCATCAATGCAAGGATAACACCTTTTTTTGAGTTATCGCTTTCTTTAATCAAATGTATAACTTCATCAGAAATTTCGGCTACTTTAATGAACCCTTCAACTATTTCTAGTCTCAATTTAAACTTCTTTAAATCATAATTAATACCATTTATATTAACTTCTTTTAAATGACTTAAATAAGCAATTAAGGCACTATAGAGATTCAAAATTACTGGACGGTTATTGTCAATTGCAACCATGTTATAACTATACGAGATGCTTAGTTCGGTTTTATTCATTAAGTATGCAATTATTGCGTCAGCATTTGCGCCGTCTTCTAATTGAATAAATATTGAAATTCCGTTTCTATCTGTTTGGTCCAAAACTTCTTTAATACCAGAAATTTTTTTATCTATTGCAATTGCATCAATGTCGGCAACTAATTTTGATTTAACAACCCCAAAAGGAATTTCAATTATTTCAATACCAATAATTTTCGATTCATCTTTTTTATCATAAACATAATGATATTTCGAAGAAATTGTAATTCTACCTTGCCCACTTGAAAGAGCTTTTTTTATTTCATTTATACCATTAATAATTGCTCCTGTTGGGAAATCTGGGCCTTTAACTATTTCACTTAATTCTTCAATTGATATTGTAGGATTTTTGATTAATGCAATTGTTGCATCAATAACTTCGCCTAGATTATGTGGTGGGATTTCTGTAGCAAAACCAGCGGCAATTCCTTTAGCACCGTTAACTAATAAATTAGGAAATAAGGCCGGCAAAACAATTGGTTCGTATTCAGAGTCATCAAAGTTTGGAGCCATTTTTACAACTTTTCTGTCTAAATCTCTCAACATCAGTTCACTAATTTTTTCAAGTCTTGATTCAGTGTAACGCATTGCAGCGGCAGGGTCATCATCAATTGAACCTTTATTACCATGCATTTCAATTAATGGGAAATTGCTTTTTCAATCTTGAGCCATTCTGACTAACGCTTCGTATATTGAACTATCTCCATGAGGGTGATAACGTCCGATAACATCCCCAACGATTCTAGCTGATTTTTTAAAAGGTTCGCTATTTTTTAAATGTAAATTTCACATTGAATATAAAATCCGACGTTGAACAGGTTTTAGTCCATCACGAGCATCAGGAATTGCTCTTTGTTGAATAATGTATTTTGAATAACGTCCGAATCTATCAGACATTATATCGGCCATATTTTTTTCAATAATGTTTTCAGTAACTTCTTGTATTTCTTCTTTTCTATCTTTTTTCATATGACCTACTTATTATTTATTTTAAAATCATCTTCTGATGAAAAATCAACATTATTGTTTATTCATTGTTTTCTTGCTATTACGTCGTCACTCATCAATGTTGAAACATTTCTTTCAACTAAAATTTCATCTTCTACATCAACTTTAATTATTGAACGAGTTTCGGGGTTCATTGTTGTTTCTCAAAGCTGGTCAGCATTCATCTCGCCAAGCCCTTTGTAGCGTTGAATTTCATAGTTGGTATATTTTGATGTAATTTCTTTTAATTGATCTTCATCCCAAGCATAAAGGGGCTGTGTATTTTTTGATTTTATTGAAATCTTATAAAGAGGTGGCAAAGCAATATAAACCATACCGTTTTCAACTAGTTTTTTCATATAACGTCAAAAGAATGTTAATAGTAATGTTTGAATGTGAGCACCATCAGTGTCAGCATCTGTCATAATAATAATTTTGTGATATTGAGCATTTTTTAAATTGAAGTCTTCACCTATACCCGCTCCGATTGTATTAATTATTGTTGCAATTTCTTCATTTGCTAAAACATCGCTTAATTTTGCTTTATCGGTGTTGATAACTTTGCCTCTAAGTGGCAATATTGCCTGGGTAACTCTATTTCTTCCTAATTTAGCAGAACCGCCAGCTGAATCACCTTCAACTAAAAACAATTCTTTAACGGCAGGATTTTTACTTTGAGCAGGAGTTAATTTTCCTGAAAGAATCTTTCTATTCTCAAATTTATTTTTAATCTTACGTGCTTCGTTTCTTGCGTTTCTAGCTGCAACTTTTGCTTCGTATGCTTTTTTAATTTTAGAAATAATTTTTTCTGCTTCAATTTTGTTTTCATTTAAAAAGAAATAAAACTTTTGACTTACAAAATCTTCAACAACTTCTCTAGCCTCTGGCGTACCTAATTTATCTTTCGTTTGACCTACAAATTCTAATATTTCTTCTGGGACTTTTAAAGAAATAACAGCAATTATTCCTTCTCTAACATCCGAGCCTTCAAAAGTTATTTTCTTAGCTAAACCATTTTTAGAAGCATAATCATTTATTGTCTTAGTTAATGCGGTTTTAAGTGCTGTTTCATGTGTTCCACCATCACGAGTCTTTACATTATTTACAAAACTGTAAATTGATTCACTATATCCATCAGTATATACTAATGCCAAGTCAATTTCAATGCCGTAATTGCACATACCAGATGCATAAAAAATTTCACTAATAAATTTTTTGCCTTCACCAACAAAATTTATATATTCTTTTATGCCATCTTTGGTTTTAAATGTTACATGTTCATCGGTATTTTCATTATAAAATTCAATAGTAAGATTTGGCATTAAAAAACTTGCTTCTCTTAATTTTTCTTTAATTATTTCGGGTGAATATTTTGATTTTTTGAATATTGAAAAGTCGGCTCAAAATTGAACCTTTGTTCCTTGTTTTGTAGTTGGAGATATCTCATGTGTTTTTTCAATAATTGTATCTTGCTGAAAGGCTGTAAAGTATGCTTTTTTATCTCTTCAAACCGTAACTTCAACTTTAGAACTTAAGGCATTGACAACCGAGGAACCTACTCCGTGTAATCCCCCGCTTGTTTTATAAGCACCATCGCCAAATTTACCACCAGCATGAAGTTCAGTAAAGACAAGCTCAACTCCACTTTTTCCAGACTCGTGTTTATTGACAGGAATTCCCCTACCGTTGTCTTCAACAATAACAGAAGAATCCTTCTTTAAAGTTACAATTATTTTATTTGCAAACCCACTCAACGCTTCATCAATAGCATTATCAAAAATTTCCCAAATTAAGTGATGAAGCCCACTTGAATCAGTAGACCCAATATACATTCCTGGTCTTTTTCTGACAGCATCCAATCCTTTTAAAACTTTTAAATCGCTAGCTTCGTAATTATTTAAACTCATATCTAAATTATAAAGAAAAAAAATCTCTTGAACTATTTAAAGTAATAAGAGACTTATTTTAGAAAAATTTTGCTTGCTATTTTTATAATTTGTGGATTTGAAAGATTAAAGTTTCTAATGTCTTTAAAATCAAAAAAAGCAATATACAAATCAAAACTATTGTCATCATTAATTGCAAAAATTAATTTAGACCCAATTGGATATTTTTCTAATTGTTTAAGATCTGGCATTTTGACGTCTAACATTGCTAAGGTCGTAGCAATGTTAGTATCGTGGCCTACAATCAATGTTAAATCTTTGCCGTTGTTGAATTCTTTTTTAATTAATTTATATATATTTTTTTTATTTGAATTTATAACATTTTTGTCGCAAAAAGCAAAGTCAATTACATAATCCTTAGCCTTACCTATTAATCTTAAATCTTTTATAAAATTATTAGATTTAAATATTGAATCGTAAGGTTCGTTGAAGTAGTATTTAACTACTAATAAATCGCTTAAAGAACAAGCCATAAAGAACTTGCCTCTTCAAAAAACTCCATCTTTATTTTCATTCAATGTAATATTGGTTTCTTCGTTTGTAAATAAACAATTTTTTAGGTCAAAAAGGTCAATAATTTTTTGATATATTTTTTTACTTTTGGCTTCATATCTTCATAGCAAATCATAATCAACGAGATTAATATCGTCAGTGTTAATGTCATATCATTCATTCTCATTTTTGAATGAATAATCTTGACATTGAATGTCGATTTTTTTAGCAGGAAAAAGGCCAATTGAAAGTAACTGCGCAGTTTGAAAAGTTCTTTGCGTTGAATTTGCCATTACCTTAACATCAATATCATCTGTTATATTGAAAAATTCTCTAATCTTTTGGGCCATTTTTAGCTCAAAAAGTGCTCCCTTATCTGTTAAAAATGCACTTCCTAAATTATCAATGTTTCAATTTACAATATCCTTATTGAAATATTTAATTGAACGTTCTTTTGTAAAAAACGGATATCTTAAACCATGCCTACTTAAAATTAATAGTTTCACAATGTCACCTCATTTTTTCAATAATTACGAGTAATTATTTTTTACATAATTGCATAATCTTATATAATTATATTGTTATATAAATAATTTTTAATTATATAAATTTTAAGGAGAAAAGATGAAAAAAACTAGAATTGCAATCAACGGTTTTGGTAGAATTGGTCGTTTAATTTTTAGAGAAATTTTTAATGACCCTGAATTTGAAATCGTTGCTATTAATGATTTAACAGATGCAGCTACATTAGCTCACTTATTGAAATACGATACAGCTCATGGAAAAATGAACGAGAATATTTCACACACAGAAAATTCTATTGTTGTAAATGGCAAAGAATATCGTGTTTATAGTGAAAAAGATCCATTAAACCTACCTTGAAAAGAACTAAACGTAGATATAGTTATTGAAGGAACTGGAAGATATGTAACAAAAGAAGGTGCTGAATTACACATTCAAGCAGGTGCTAAAAAGGTATTTATCACTGCTCCAGCTAAAAGCGAAGGTGTTAAAACAGTTGTTTATTCAGTAAACGAAGATATCATTACGCCAGAAGATAAAATTTTATCAGGTGCTTCATGTACTACTAACTGTTTAGCTCCTATTGCCAACGTATTGGAAAAAAACTTTGGTATTGAAAAAGGATTTATGACAACAGTCCACTCATATACAGCAGACCAAAGATTACAAGATGCTCCACACAAAGATTTAAGAAGAGCAAGAGCAGCTGCTTCAAACATGGTTCCAACAACAACTGGTGCTGCAATAGCAATTGGAAAGGTTATCCCTTCATTGGATAAAAAACTAAATGGACTATCTTTAAGAGTTCCAACAATAACTGGATCATTCGTTGATTTAACTGTTGAATTAAAGAAAGATGCTACTGTTCAAGAAATTAATGAAGCAATGAAGAAATCTGCTTCTGAATCATTAGAATATTCTACTGAACCAATTGTATCAAGTGATATTATTGGTGCAAAAGCAGGCTCAATATTTGACTCACTTTTAACAAGTGTGTTAGAAGTAGAAGGCAAAAAACTATACAAAGTTTATGCATGATACGACAATGAATCATCATTCGTTGCTCAATATGTTAGAACATTAAGACATATTGGAAAATTAACAAAATAGTTAAAAAAAATATTGGATAAGAAATGAAACTTTCAATAAAAGAAATAATTAAAAAAATTAAATTACTTGAATTGGATCTTGAAGATTTAAAAAAAGAAGAATCCAAGGAAAACTTCATTGTTTATTTAGAAAACAAAAGGCCAACTAACATTGAATATGATTATCAAGATTATTCAAACAAAATTAAAAATTTATATAATGAGATTTTTAAATTAAGAACATTGGTTCAAGTAACTAATATAAACACAATAACATCATACAAAAAATATTCTATATCAGAATTAATTATTTTATTAGCTCAAAAAAGTAATCAATTAGAAAGATTGCAAGAAATGCGCGATTGCAAAAAAATTTCTAGGGTTACTACAAACGATGGCCAAAACGAATATACGGAATATCTTTTTGACCCTAAAATGGTTGCAATTGAACTTAGAAAATTAAACGAAGAAATTTCTGAAATTCAAATTGCAATTGATAGTGCAAACATTAATACATTAGTTGAAGTTAAGTAATTAACTTTCCTTAATAAATTTGCTTTATTTTCTATAATTTTTTGTTTATTGTTATAGATTTCTAAATTATTAACCTTCCGTTTTAAGTTTGTGTTTATATGATATCGAATTTACATACTTAAATTAGCTAATTTATTTAAAAACTTCTACATTTAGTAAGGGTTAGAAAATGTAAACCACCATTTGAGGTGGTTTTTATTTATATAAATTTATAAATATTAATAATTAATAATATAATTAGATAATTATGAATACAACAAAAGTTTACATAGATTTTGAAGCAATTACCAACCCTTTTGCAAGATTAGTAAATTTGCCAAGCGGAACTCCTTATGCCTATACATTAGGTCTTTTAAATATTCACGGCCAATATCAAATTAAAACATTTATTATTGATTTTAAAGAACATCATAATTTAAATTCAATTTGAACTGCCATAAAAAATGCAATAGTTTCTGACATATTAGAGATCAATAATACTGTAAATTTAAAGGAAGTTGTATTTGTAGGCCACAATCCTGTTTTAGAAAATAAATGCTTAAAAAAATTATTTCCTGAAAACAATGTTGAACCTTTAATTTCCAATACAATTGTGTCATTGTCAAAATTAACAGGAAAAATTTTTAACATTCCATATTTTAATAAAATAAAAAAAATTATCGCTGATTCTGGTGATAGCGAACTTAATATGAGAATTAAAGATAGAAATGGCGTAATTGCCGCATTTGCTGGGTATTGATTATATACAAAATCAATTAAAAATTTACGAGCAAATGACAGAAGAAAAAGATTTTTAATTAAGATGAATAAAAATCTTTTGTTAAAAGAATTAAAACGTTATTCTCATGACGATGTCAATAAAATGCTTTATATTGTTTCTCATCCTGATGAAACAGAAACTTTACTAAAAGAACTTTTATACAAACGTGAACTAATAAAACAAATAAAAAATTTGGAACTTGACGACAATTTAACAATTAAAGAAATTAAAGAAAAAATTTGAACATTATAATTTCTAAAAATAAAGGATATTATGAAAAATAAGCTTATTGAAAAAAAAGGTAAGTTTTCAATTAAAAAATTTTTCCCTCAAAGCAAGTTTGATTGAAAAATTTATATTATAAAAACATTGCCAATAATAATTGGCGAAATTATATTTAATTTAAATGGGCTACTTGATAATTTCATGGTTAGCCATATTGATGGTGGAATTGATGCCTTGGCTTATGCTAATACATATACGGGAATAATTTATACAATTTTCTTCTCTATTCAAGCAATTGGAGCAATGTTCGTGGGTCAATATTATGGCCGTAAAGATTTTGATAAAGTAAAGCAGATAATGAATTTAAGAATTTGAATGAATCTAATTATTACCTTAAGTTTTGCAATTCCTGCTTGAACTTTAGCAAAGCAAATGGTTCACTTGATTGGTGGGCAAGGAATAAAACCTCAATCTGAATTAGCATCTACATATTATTTAATGTTTATTGCCATAAACTGAGTTTTAACTTCTTTTAATTTTAATACAAATATGTTGCTAAATGAAACTGGACATTCAAAATACGCTTTTGTTTCGGCATGTTTAACAATGCTAACAAATGCTTCAATTAATTCTATTTGTTTATACGGATTTCATAAACCTGCATATTATGCTGCCTTTGGTTCAATTATTGCTGCGACAGTTTGCTTTACAAGTGATTCTTTATTTACGTATTACAAAGAAAGAAAAATTTTTATTAATGTATTTAAAATGTTTCATATTACAAAACCAATTGCAAAGCAATTATTAAAAAGAATTCCCGCCATGTTAATTGCCATTGCTGGAATGTTAACTTTACCTACAAGAATGATTATTTGAAGCCATGCATATCCTGATAATTTATCAGGCGGTTCAGGAATTGGCGAAAAATGAATGGGAATTAATGCTGTTACAATACTTGGTTTAGTCGAAAGCCTTTCGTCCGTCGCCTCGGCAATAACTTCTGTTTGTGCAAGCAATGTTTCATTTTTCGTCGCCACAAGATTAGGAGAATCTGATTTTGAAGGTGCAGATAAGCACGCCCATGCATTGCGGGGTTTTCATGCTTTGGCTGGATGTTGCATGTCTATATTAATGATTGCGGTTGTTTTTGGAATTGCATATTCTCCTACTACAACAAAAGGTGTTGGTATCGGCGTCTCTCAGAGATTTTATGATGTGCAAAGAAAAGATTACATTATTGAAATATTAAATAAATCTGATATTTTAAAAAATAGTGATTTATTAAATTCACTTGGCAATGATTTTACATTCACAAAAGGTCAAAATTTGGCTTATGACCAATGGGTGGCAAGCGCAGTACTGGCAACTTCAAAAGAATTCAAAGAAACATTTTTGTTATGTAGTGCAACCTTTATTTTCTTTAATCCTATATGATGCTGATTTTATACAAGCGCTGCTTTACCTGCTGCGGGCGGTAGAAATATAATTGGTTCTATTACAATATTAGCAACACAATGAGCATCATTTATATGATTAATTATTATTACTTATGGAATTGTTATTCCATTGCATAAAACTTATACTGGATATAGTGATTTATCAATTCCTCTAGAACTTGCTTATTTTATGTTATTTGCATTTGACTTTATTAGATGATTGATGTTTGAAATAGTAGCATTAAAGACTGATTGAAAGAGAAATATTACAAATGAAATTGAAAAACAAGATCCAAACTTTCCAAGTAATATAGCAGAAGCAACAGCAACACACGATATTGTTCATAAACAATAAAAAGGAGATTTAAGATGTTATATGATTTAGTTTCACCATATAATTCAGGACATTTAAAGGTTAGTGATATTCATAGTATTTATTATGAAGAATCAGGAAATCCAAATGGCAAGCCAATTTTGTTTGTCCATGGTGGTCCAGGTGGGGGAACCAAACCTTCATGTCGTCAATTTTTTGACCCTGAATTTTATAGAATTATTTTATTTGATCAAAGAGGATGTGGACTTAGCATACCTAGTGCCGAAATCAAACAAAATACGACCCAAGATTTAATAGAAGATATTGAAAAATTACGGAAATTTTTAAACATTGACAAGTGAATATTGTTCGGGGGGTCATGAGGTTCAACTTTAAGTTTACTATATGCAATTAATTATCCTCAAAACGTTTCAGGAATAATTTTGCGGGGAATATTTTTAGGTAGGGATGAAGACAATTATTGATTGTATCAAAAAGGCGCTTCTTATTATTTTCCTGCTGAATATGAAGAATATTCAAACTTTATTCCTGAAAATGAAAGAGGCGATTTAATTAACGCTTATTATAAATATTTAAATTCAAGTGACCAAAATGTTGCAGAGCAAGCAGCATATCATTGAAGCAAATGGGAACTTGGATTGGTAACAATGAAAAAATTGCCTAATTTAGAAGAAATTTTATCCGATAAAAAAAGCAATTTAGAATTAGCAAGATTAGAAAATTATTATTTTGTTAATAAAATATTTTTAAATGACGATAATTTTATTTTAAATAATGCAAGCAAGATTAAAGAAATCCCAACAATATTGATTCATGGTCAATTTGATATGGATTGTCGCCCAGTTGGCGCATATTTATTGCATAAAGCCTTACCAAAGTCAAAGCTTTGAATAATTCCAGAATCAGGCCATTCAGGTCGTGATGGACATATCAACGAAGCATTAATGCAGGCAACTGAATACTTTAAAACAATTAAATAAGCGTTTGCTTATTTTATTTTACTAAATGATTATGAAACAATAAATGCAATATTTCTTATAATTTAAACAGACATTTAAGGAGAGAAATGAATTCAATAAATAACGTTGCTAAAAAATTAAATTTAAGCGAAAATCAAGTAAAAAATACATTATCTTTGTTATCAGAAGGGGCAACAGTACCATTTATTTCAAGATATAGGAAAAATATAACTGGTGGTTTAGACGAAGATCAAATTACAAAAATAAATGATTTATATGTGTATGATGTTGAATTAAATAAAAGAAAAGAAGCCATATTGAATATATTGGCCGAAAATAAATTATTAAATGATGAATTAAAGCAAAAAATTGAAGCTGCTGATACAAAACAAGCAGTTGAAAATATTTATGAACCATTTAAAATAGGTAAAAAAACAAAGGCATCAGATGCTATTGCTCTTGGTTTAAGTCCATTGGCTGATGAAATTTTAAATAATAGTGATGACAACTATAATCTTTATTATGCTGCTAAGAAATATATTAGTGATAAATTAAAAACTGAAGATGAAGTCTTACAACAAACTAAATTTATCATTGCTCAAAATATTTCTCAAGACCCTTCTACAAGAGAATATGTAAAAAATCAATTATGAGATTATGGAGTTATTGAAACTAAATTAAAGAAGAATGCGATTGATGAAAATGAAACTTTCAAACAATATTATAATTTCTCTGAAAGAGTTAATAAAATTCCAAATCATAGAATTTTAGCAATATCAAGAGGGGAAGATAAAAAAATTCTTTCATATGATATTACTTATAATGAAAAGAAAATTATTTATGATCTAAATCAAAAGTATTTCAAATCAAAACGAACTGCTAAATGTATTCAAGAATGTATTTTAGATGCATTAGACAGACTAATTATTCCTTCAATAATTAGAGAAATTAAAACCGAATTATTTGAAAGAGCTGAGGCAGAAGCTATTAAAGTATTTGCTTCAAATGTTGAACAAATGTTATTGAGTCCAGCAACTAAAAATAAAAGAATTTTAGCAATAGACCCTGCTTATGTTAATGGATGTAAAATTGCAGCCATTGATCAAAATGGTAACTTTTTAAAAGAAGGAATAATTTATCCTCATAATCATAATCAAGAACAATGATTTGAGGCAATTAATACAATTAATAAATTTATTGATGAATACAAAATAGATTTAATTGTTATTGGTAATGGAACAGCAAGTAGAGAAACTGAATCGTTTATTGATAAATTATTGCAAAAAAGACTTGAAAAAAATCCTAATGAAAAGATTAAGTTTGTGATTGTAAGTGAAGTTGGTGCTTCTGTTTATTCAGCTTCAAAAATTGCTCAAGAAGAATTTCCACAATTACATGTTGAATATAGATCAGCAATTCATATTGGTAGAAAGTTTCAAGACCCCTTAAATGAATTAATTAAAATAGATCCTAAATCAATTGGAATAGGACAATATCAACATGATGTTAACCAAAAAGAATTATCAAAACAATTAACCGAAAAATTAAATAAAGTTGTTAACCTAGTTGGTGTTGATCTAAATACAGCAACAAAAGTGATTTTATCTTATATATCAGGATTAAGTAATACTATAGCAAGCAATATTGTAAACTATAGAGAAGAAAACGGTTCATTTAAATCACGCGAAGAACTTAAAAAAGTGAAAGGACTTGGGCCAAAAGCATACGAACAATCAGTTGGATTTTTAAGAATACATGATTCTAATAATTTTTATGACAAGACTAATATTCACCCCGAAAGTTATAAATTAGCAGATACCATTGTCAATAAGCTAAATATAGATTTAAATAATATTGACAAAGAAAAATTATTATCAGTTGACAAAGAACAACTTGCAAAAGAATTAAATTCTGATAAATACAGTATTCAATTAATTATAGAATCATTGATTGCACCCGAAAAAGATATTAGAGATGACAAACCAGGATTTTTAGTATCAGATAAAGTCTTAACTATTGATGATATAACTCAAGGTCAAATAATTAAGGGTCAAATTCAAAATGTTACCGATTTTGGAGCCTTTGCGTTTATTGGATTAAAACAAAATGTTTTGATACATATAACCAATATGAAGAAGAAAGAAAATCAATTTATTAAACACCCTTTAGATGTATTAAATGTTGGTGATAATGTAAACATACAAATAATTTCCATTGATAAAGAACACAATAAAATTCAAGGAAAAATTATTTGAGATTAATTAATAATCAATATTGCAACATTGCTATTGAAAAAAATAGCAATGTTTTTTATTGTAATTTTGTAAAATTTATATATAAAAGTTATTTATTGCATTGGGGGAAGAGATGGCTATTAATAATCAAGAACGTGACGAATTACATAAAAAAATTTGAGATATAGCAAATAGATTACGCGGTTCAATTGATGGTTGGGATTTTAAACAATATGTTTTAGGAATTATGTTTTATAGATATATTTCTGAAAATATTGCAACATATGCAAACAATCGCCAACGTCAAGCAGGAATTGAAGACTTTGATTATACAATTTTATCAGATGAAGAAGCATTGACGGGCAGAGATGATTTAATCAATGAAAAAGGATTTTTTATTTTACCTTCTGAATTGTTTATTAATGTTGTTAAAAATGCTACAACAAATAATTGTTTAAATGAAACGTTGGACAATATTTTTAAAAATATTGAATCATCAGCAAAGGGGCAACAAAGTGAAAATGATTTTTCAGGTTTATTTAATGACGTTGATGTTAATTCTCAAAAATTAGGTCGTAGTGTTGATGAAAGAAATAAAAAATTAGCAGCAATATTGCAAGAAATAGCAGCCATGAAATTAGGTAATTACCAAGATAATTCAATAGATGCCTTTGGTGATGCTTATGAATATTTGATGTCAATGTATGCATCAAATGCCGGAAAATCAGGGGGTGAATATTTTACTCCCCAAGAAGTTTCAGAATTACTTACTAAAATAGCAGTTTTTAATAAGAAAAAAGTTAATAGAGTATATGACCCTGCATGTGGATCGGGTTCTTTGCTTTTACAAACAATCAAGGTTCTTGGCAAAGAAAACATCAAGGATGGATTTTATGGCCAAGAAGTCAATTTAACAACATACAACTTATGTAGAATAAATATGTTTTTGCATGATATAGGATTTGATAAATTCAATATTTACAACGGCGACACATTGCTATCTCCAAGCCCTGAACATCAACGCAAAGAACCATTTGATGTTATTGTTTCAAATCCTCCTTATTCAATTAAATGAGAAGGCGAAGATAACCCTTTATTAATAAATGACCAAAGATTTTCACCAGCCGGAATATTAGCTCCAAAAAGTAAAGCTGATTTCGCTTTTATACTTCATAGCCTTTCTTGACTTGCAACTGATGGCGTAGCAGCAATAGTTTGCTTTCCAGGCATTATGTATCGTGGGGGCGCTGAACAAAAAATTAGACAATATTTAGTTGAAAATAACTTCATAGATGCAATAATTCAACTCCCAGGCAACTTGTTTTTTGGAACAAGCATTTCAACATGCATAATGGTATTGAAAAAATCAAAAATCGACAATAATATCTTATTTATTGATGCCTCTCAAGAATTTTTAAAAGTAACAAACAACAACAAATTAACTAGTCAAAATATTGACAATATTATTGATTATTATGGTCAAAGAAAAGACATCTCTTATATTTCAAAATTAGCCAATGTTGAAGAAATCAAGCCCAATTCATATAATTTATCAGTCAATAGTTATGTTGAAAAACAAGACACTTCAGAAAAAATAGATATCAATGTCTTAAACTCTCAAATAAAAGAAACAGTTTCCAAGATTGAAACATTGAGAAGTGAAATTGACAAAATAGTTGCTGAATTAGAGGAATAGTTATGAACAAAATAGAAAAGTTAATTAAGGAACTATGTCCTAATGGAGTTGAATATAAAAGGTTATGAGAAATTGTTGATTTTGACAAAAAATTTAAAGGAGTAGATAAAAACAAACAACAAAATATATTATCATTTAAGCATGTTTCTGCAAATGAACTCAAGCTCTATAAAAAAGAAGAAACGGGAAATGTAAAATTATTATCAACAGGCCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTATGGTGAGATTATTTCAATACCATCTGGTGGCGCGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTACTTTCTAATTGCTAACAAGGAAAGCATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATCGAGTTGCAAATTCCACTTCCCCCTCTTGAAATCCAAAATCAAATTGTTAATATTTTAGACAAGTTCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCACCGAGTTGACCTATAGAAATAAACAATATAATTACTATAGAAATAAATTATTAGACTTTGATAATAATAAAGAATTATTAAACAAGATAATGAATAAACAACAATGCAGTAATAGTATAGTTGAATATAAGAGGTTATGAGAAATTGTTGATTTTGACAAAAAATTTAAAGGAGTAGATAAAAACAAACAACAAAATATATTGTCATTTAAGCATGTTTCTGCAAATGAACTTAAGCTCTATAAAAAAGAAGAAACGGGAAATGTAAAATTATTATCAACAGGTCAATATGATGGTTTTACCTCCTATGTTGAAAATGATAAAAACATTAATTACGGTGAGATTATTTCAATACCTTCTGGTGGCGCGCCATTAATAAAATACTACAAAGGATATTTTATTGATAGTTTAAATATATTATTATCATCAAAAGATGTTAAAAAATATAGTCTCAAATTCTTATATTATTTTTTAATTGCTAGTAAGGAAAACATTGAACAAAATTTTAGGGGTTCCTCAATAAAACACCCTGATATGGAAAATATTATTGAATTACAAATTCCACTTCCTTCTCTTGAAATCCAAAACAAAATAGTTGAAATCCTTGACAAACTTGAAACATATACAAAAGACATTCAATCTGGCCTTCCTCTTGAAATTGATCAACGTAAAAAACAATACGAATATTATAGAGATAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTATGAGACAAAATTGTCAATATAGTTGAGTGCTTATCTATATCAAAAATTTTTAGAGAAATAAAAACCGGAAAATTAAACGCAAATGCAGAAACTCCAAATGGAAAATATGCGTTTTGAACATGTGATGAAAGACCAAAATTAATAGATGAATATGCCTTTGATGAAATGGCAATATTAATATCAGGAAATGGAAGTAAAGTAGGTCACGTCAATATTTATAATGGGAAATTTAATGCATACCAAAGAACATATATTTTGTTAAAAATAAACCATTTTGTTCTTTGAAAATACGCTTACTTTTATTTAAAGAGTAACTTAAAAAATTATATTAATGTTTATAAATTAGATTCTGGAATTCCTTACATTACACTTCCAATGTTACAAAATTTTGTTATCCCCATTCCCCACATTTCAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAATCTGGACTTCCTCTTGAAATTGACCAACGTAAAAAACAATACGAATATTATAGAGACAAGTTGTTAGACTTCAAAGACCTTGCGGGGGGGGTATTAAGTAAAAATTATTTACTGCTTTTAAATGAACTTTGAGACAAAATTGTCAATATAGTTGAATATAAAGAATTAGGAACCATAATCACAAAGTCTCCCAAATCTCCATTTGGAGTTGCAAAACTCTTAAAAATGAACAAAGGGGATTATTTAGCAATAACTTCAGGAAATAAATCATTTTATGTCTCTGATTTTTTAGTTGATGGAGAATATATATTTGTTAATGATGGTGGGCAAGCAGACGTTAAATATAATCAAGGAAAAACAATGTATTCCGATCATGTGTTTGCGTTTAAAACAAATGAATGCAACACAAAATATCTTTACTTTTATTTATTGAATATTTCTAATTTTATTAACGAAAAATTATTTGTTGGTAGTGGATTAAAAAATTTAAATAAAAAAGAATTCTTGAATCTTAATATTGCAATTCCTCCTATTGAAATTCAAAATAAAATAGTTGAAATCCTCGATAAATTGCAAGCCTATACCAAAGACATTCAAACTGGTCTTCCTCTTGAAATTGACCAACGTAAAAAGCAATACGAACATTACAGGGATAAATTATTGAACTTTAAAGAAAAGAATACTAAAATTTAATTAGTAGCAATATTAAATAAGGGTGTAAACTATGGAAAAATATAACATTATTGTTGAAAACGAGAATGATACAGTTGTTTCTGAATATAAGCCTCTAGCGAATAAAGCAAAAGATTATCAAACAGAGAGTGAACTTGAAAGTCAATTCATATCAATGCTTAAAAACCAAGGATATGAGTATTTGAACATTCATAATGAAGAAGATTTAATTAATAATTTAAGACAACAAATTGAATTGTTAAATAAAATAAATTTTTCAGATAATGAATGAAAAAAATTTTTTAATGAAAATATTGCAAATAAAAATTTTTCAATAAAAGATAAAAGTAGAATAATACAAGAGGATTATCGCCAACTTTTAACTAGAGATGATGGTAGTCATAAAAATATTATTTTAATTGATAAAGACACTATTCATAATAATAAACTTCAAGTTATAAATCAATATGAAGAAGAAACAGGCAACTATAAAAACAGATATGATGTTACTATATTGGTTAATGGCATTCCAATGGTTCATATTGAATTAAAAAGAAGAGGGGTTGCTCTAAAAGAGGCTTTTAATCAAATCATTAGATATCAAAGAGATTCGTTTTGGGCTAGCAACGGCTTATATGAATATGTACAAGTGTTTGTTATTTCAAACGGGGCAAATACCAAATATTATTCAAATACAACAAGATTACAACATGTTAATAAAAAATCAGATATGATAGATTCAAAAACTTCTAAGAGTTTTGAATTTACACATTTTTGATCTGATTCAAAAAATAACCCTATTCATGATTTAATTGATTTCACAAGTACCTTTTTTTCAAAACATACGATTTTAAATATATTGACCAAATATTGTATATTTACATCTGAAGAAATGTTGCTAGTAATGAGACCTTATCAAATTGTTGCTGTTGAAAAAATAATCAATAAAATAAATATTTCACACAATTATAAAAAATATGGAAGTATTGCAGCGGGTGGCTTTATTTGACATACTACAGGAAGTGGTAAGACTTTGACCTCTTTTAAAACTGCAAAATTAGCCTCACAATTGCCTTATATAAATAAAGTAATTTTTGTTGTTGATAGAAGAGATTTAGACTATCAAACAATGAAAGAATATGACAAATTAGAAAAAGGCGCTGCAAATTCAAATCGTTCTACATTAATATTGCAAAGACAACTAGAATCTAATGACCCTAATAAAAAAATAATAATTACAACTATTCAAAAATTATCAATTTTTATAAAAAATAATAGAAATCATCCTGTTTTTGATAAAGAAGTTGTTTTAATATTTGATGAATGTCACCGCTCTCAATTTGGTTCAATGCATACAGCTGTTATTAAAAATTTTAAAAAATATTATTTATTTGGTTTTACCGGAACACCAATTTTTGCAGCTAATATCAATAAATCAAGTCCAGAAGTGATTAAAACAACCGAACAAGCTTTCGGCCAAAAATTGCACACTTATACAATAATAAATGCAATAAATGATAGAAACGTTTTGCCATTTAAAATTGAATATATTAAAACAATTGATGTTGATAAATCGCTAAACGATAAAGAGGATCAAAAAGTAAAAGATATTGATCGGGAAAGTGTTTTTTTAGATCAAAGAAGAATTAAACTAGTTGTTGAATACATATTGAAACATTTTGATCAAAAAACTAGGAGAAATTCCAATATAATTTATAGCCATTCAATATCTGATAATTTAGATAAAACTATTAAAAGAAATGTGAGCGGTTTTAATTCTATTATGGCGGTTTCTTCAATAGAAATGGCTAAAAAGTATTATTTAGAATTTAAAAATCAACTTGCTCAAGACCCTAAAAACAATCTAAAAATTGCTTTAATATATTCATATGGGGTTAATACCGAGGAATCATATGGCGGTTTAATCGATGAAGAGGATAATGAAAATACATTGCAATTAAGTAAGCCAGATAGAGATTTTTTGGATGGTGCTATTAAAGATTATAATAATATGTTCAACACAAACTTTAATAGTGAAGGTGAACAATTTCAAATGTATTATAAAGATGTTTCTTTAAAACTAAAAAATAAAGATTTAGACATGCTGATTGTAGTAAACATGTTTTTAACTGGTTTTGACGCCCCAACATTAAATACATTATGAGTTGATAAGAAACTAAGAGAACATGGTTTAATTCAAGCTTTTTCAAGAACAAATAGAATTCTAAATGATAAAAAGGTATATGGAAATATTGTATGTTTTAGAAATTTAGAAAAAGAAACAGACCAAGCAATTTCAATATTTGGCGATGGAGAAGCTAGTGGAATTGTTAAAATCAAAAATTTCGTTGACTATTATTATGGCTATAATGATAGTCAGCAAGTGCATCACAATGGTTATTGCGATTATATTCAAAAATTAAATAATGAATTTCCTTTAGATAATTGAAATGTAATAAGTGAACAAAAAAATAAAGAATTTATTATATTGTTTGGTAAGGTATTAAAATTAAAAAATATTCTTGCTTCTTTTGACGAATTCATTGGTAAAGAAATTTTAAATGTTAGAGATTTTCAAAATTATTCAAGCAAATATTTGGATATAAAAGATGAATTTAAAAAGATATATAAAGAAGAAATTGCTTCAATTAACGATGATGTAATTTTTGAAACTGAATTATTAAAGACTTACGAAGTTAATATTGATTATATATTGCAAAAGGTAGAAGAGTTTAGAGGAAAGACAATTACTGAAATTAACAATTTAAGAAATGAAATATCTTTAGCTTCTTCTTCATCGCCTGAATTAAGAAGTAAAAAACAATTAATTGAAAAATTCGTTTCAACAATTAATAATAATGAACAAGATATCCCAACTAAATTTCAAGAATTTATGGAACTAGAAAAAAAGAAAGAGCTTGACAAAATTATTAGTGAAGAAAATTTGGATTTTGATAAAACCCATAAATTTATGGATGATTCGTTTGAGTTAGGAGAAATAAAAACTGCTGGTAGTGAGATTGGAGATTTATTGCCTAAAATGCCGTTATTTGGTTCAAAAGGAAAAGAAAGATCTATTAAAAAGAAGGCAGTTTTAGAAAAATTTGAAAATTATTTCCAAAAATTTTATATTTAAATTCTAATATTTTTGGTTTATAATATAGTTACGTTTTATATTGATATATAAAGGGTAGGCTAAATGCCTCCCTTTTTACTTAAAATAGATAAAAAAGGAATGAAAATGAGTGAAATAAAATTAACAGCAAAAGAAATTTACAAACAAATTTATAACCTTTCACAAATTAAAAAAATAGATCAAGCAGAAGTTGTTGAAGCTTTTAAAAATACAGTTACAAAGTTAATAACTGAAACTTATGACGAAGAAGCAGATCTTGAATTTATTTTCGATCAAGAAAATGACAATTTTGCAATTATTAATCATAATAAAGTTGTCGTTGGCGATCCAATAACAAGTGAAGAAAAAGATCGCTTAACAAGATGCATAGAAATACCGTTAAGTGATGCTAAAAAAATAGATCCAAATGCAGAAATTGGAGATTCCCTTTCTCAAGAAATTAACTTTGAATATTTTAGTAAAAAAGACTATAACAAAATTCTTGCTAATTTCAGTCAAGACATTAAAAATTTAGAAAGAAAAATGACTTGTGAAAAATATGCAAGTGAAGTCGGAAATTCAACTAAGGCAAAAATTGTTTCTGTTAACAAAGGAAAAGTAAATCTTGAATTAAGAGATGGAACTTTGGCATTTATGCCTTCTAATTTTGTAAACCAAAGAATAATTTCAAAATTAAATCCAGGCGATTGAATTGATGTTGTAATTGAAGAAGTAAAAGAAGAAAACAGTAATGCTCAAATAATTGTTTCGTCAGTTGAATCAAGATTACTTCAAAAATTATTTGAAATTGAAATTCCTGAAATTTCACAAGGCTTAATTTCAATAGTTAATATTGCAAGAATTTCTGGAGAAAGAGCAAAGGTTTCTATTAAAAAATCTAATGATGCTCCTGAAACAATGGAAGAAATTGGCGCAATAATGGGACGTGATTCTGAAAGAATTTCAACAATATCTAGAAAACTTAATGGTGAAAAAATAGATGTAGTTTTATATTCAGATGATATAAAAGTATATATTAAAAATGCATTAAGTCCAGCAAAGGTTATTGATTTAATTGAAGTTCCATCAAAAAATTCTTATCCTTCATTTAATGTTATTGTTCCAACAATTCAACATACGCTTGCTATTGGAAAGAAAGGCCAAAATGTTTCTTTAGCTTCTGAATTAGTAAAAGCAAAATTAGATATTTTAAGCCAAGAACAAGCAGACGAAAGAGGCATTGAATACAATTTTGAAAATGGTAATATTACAAAAGAAGAAATTGAATTACTTGAAAGTGGTAAAAAACTTCATCACTCATTTAAACACCCAAGAACTATTGGCAATAAAAATGCTACAAACAAATTTAATACGTTTGAAAATAGTTTTAATATTGATGAATTTGATGAAGACCTTGCCGAAATGAGAAGAAAAGCTCAACAAAATGATAATATCTTTGAAAGACAAATGTTTTCAAATAGTCTAGATGAAGATTTAGAAAGAACTTTATCAGAACTAAATGACGACAAAGAAAATAATGTTGATGAAAATGACCTTGATCCTTATTCAAAATCTTTTGATGAATTAAATGAACAAAAAAATGATGAAGAAGAGGATTACGAAAAAATTACAGCAACAAAAATGAAAGATTTTAAACGTGATGAAGATTTAAGCAGTGGATTAGAAAACATTGATTTAAGTGATTTGGATGATGAAAAATGGTAAAGTCTATACAAAGAAAGTCAATTGTTGATAATAAAATATATGATATAGATTTATTGCTTAGATTTTCTAAAGATAAAAACAATTTAATTAGCTTTGATCCTTACAAAAAGAACCCCGGAAGAGGCGCATATTGTCTAAACGATAGTGAACAAATTGAAATTCTATTTAAAAGAAGATTATTAAATAAAGCATTTAAGCAGAATATAGATATTCAAAAATACAATGAAATAAGGAAAGAGGTGGATGAATGAGTAAACAAAATATCCAAAAACGAAAATCAAACGTTAGCTCATTAAAAGAGCAATTAACTGCTGGTGAAACAAAATTACAAGATGGCGTTTTTATATTTAATGGGCCATTGACAATTTCAAAATTTTCTCTTAAAATAAATAAAAACCCTACTGAAATATTGACCTATTATTTTAAAAAGGGAATAATCAAAACAATTAACACAACATTAAGCGAAGATGAAATAGCAGAACTTTGTTTTGAATTTAATTTAGATTTTAAAAAAGAAGAAAATGTTGATGCTCAAAATTTGTTTAATAAGCTTGAAATTGTTGAAGACCCAAATGATTTAGCTTCTAGACCCCCAATAGTAACAATTATGGGACATGTTGACCATGGTAAGACTACATTAATTGATAGAATAAGACATAGTAATGTTACTGAAACAGAATTCGGTGGTATTACTCAACACACCGGAGCATATCAAGTTGAATATCAAGGACGCAAAATTACATTCTTAGATACACCTGGTCATGAGGCTTTTACTGAAATGCGTTCAAGGGGAGCAAAAATTACTGATATAGTAGTCATTGTAGTTGCGGCAGATGATGGAGTTAAACCTCAAACTATAGAAGCAATAGACCATGCAAAAGCAGCCAATGTGCCAATTATTGTTTTTGTTAATAAAATGGATAAGCAAGGAGCCGATATTGAAAGAATAAAAAGCGAACTTGCTGATAACAATGTTTTATGTGAAGAATGAGGTGGAAACTACCAATTTGTTTATGGATCGGCTTTAAAAAATTCTAATATAAATGAATTACTTGAAGCAATATTTTTACAAGCTGATTTACTAGATTTAAAGGCCAATATAAATAGAGATCCTATCGGAACAATTATTGAATCTAGAATTGACAAGGGACGTGGAGTTGTTTCAACCGTTATTGTTGAAAGTGGAACATTGCTACCTCGTGATTTCATTGTAGCTGGAGGGCAATATGGCAAGATTAGAAGTCTAACCTCTATTGATGGTAAGCCTATACAAAAAGCATTGCCTGGTACTCCATGTATAATTACTGGTTTAAGCAAAAACCCTCAATCGGGTGATAAATTTGTAGCTATTAGTGATGAAAAATTTGCTAAAAAATTAGCATCTGAAAAATCGTATTTAGATAAATGTAAAGAATTAAATGAAAAAAATGCATTTTCTATACAAGATGGAATAAAAACTGTAAATGTCATTGTTAAGACCGATGTTCAAGGAACAGCAGAAGCAATTAAACAAACATTGCAAAAAATCAGAAATGATGAAATTGCAGTTAACGTTATAAGAGCAACCTCTGGCGAAGTAACGAAAGCAGATATCTTGCTTGCTGAAACATCTAATGCAAGAATTTATTTATTCAACAATACAATAAACAATGAAATTAAAAAGGCTGCCGAATTAAAGAATATAAAAATCTTTAAACATAATATCATTTATAAAATGGTTGAAGAATTAGAAACTTTAATAACAACCTTAAAAGAACCTGTTTATGAAGAAAAACTAATTGGATCTGCAACCGTCCTACAACTATTCTTTTATTCAAAAATAGGCCAAATTGCAGGATGCATGATGCAAGAAGGCATTGTTAAGGAATTTAGTAAGGTCGAAGTATATAGACGCAATAAATTAATTTTTAAAGGACAAATTATTTCTCTAAAAAGAGGAAAAAATGATGTCAAATCTGTTGAAAAGGGTTTTGATTTTGGAACTCATGTCAAGGATTTTGATAAAATAGAAGTAGATGATGAATTAAAATTTTATGAGGATGTATTAGTCAATGAACACAATTAATCACGAAAGAAAAACACAATTACTATTAGAATTAATTGGAAATAGTTTTTATGAATTAAAAGATTTTGATGTAACAAACATTTCAATAAATGATGTTATATTATCAAATGATGGTTCTCATGCCAAGATCTATGTAACTTTATTTAAAGATCAAAAAAGATATTTAGAAAAATTAACCACAATGACACCATTTATTCGTTCGGTTGTAGCAAAGAATTGAAAATATAAAGTTCTACCAGAATTAATTTTTGAAATTGATACTGTAGAAGAACATGCAGCTAAAATCGATAAGATTCTAAATAAGATTGAAGAAGAAAAAAATAATTAATTAGAAAGCAAAGGAAAAATATGACCAATAATTTTGACGCTATTGTAATTGGCGGTGGTCATGCTGGAATAGAAGCAACTTATGCTTTGGCAAAAAGAAATTTTAAAGTCGCATTGGTAACTTTAAACATTAATAGACTTGCTATGCTTCCTTGCAACCCCTCAATTGGTGGTTCTGCAAAAGGAATAATAACACGTGAAATTGATGCCCTTGGCGGAGTTCAAGGGTTTTTTGCTGATAATGCAATGATTCAAATTAAGATGTTAAATACATCTAAAGGCCCAGCAGTTTGATCATTAAGAGCCCAAATCGATAAAGAAAAGTATTCAGAAATAATTTTAAAAGACATTGAAAAACAAAAAAATATTACATTGATTCAAGATGAAGCAAGCGATTTAATTGTTGAAGATGGAACTTGTGTTGGAATTGAAACACTTGAACATGGTAAACTATATTCAAAAGTTGTTATTATGACAACTGGTGTTTATATGAATTCAAGAATTTTGCGCGGTGAAAATATAAAATATGATGGACCCGATGGAGAAAAGACAAGTTCAACACTTTCAAAAAATTTAAAAAAATATGGTTTTGAAATAATAAGATTAAAAACTGGAACACCTTGCAGAATTTATACAGATTCAATAGATTTTTCAAAAGTTGAAAAAGAAGTTTTAGAAAAAAATGAACTTTGTTTTTCAAGCCGTAGTAATAAAAAACTAGATGAACAAACTTGCTGTTATTTAACACACACAACCTTAAAGACAAAAGAAATAATCTTAAAAAACTTAAAACGTAGTTCATTATATTCTGGAATCATAATTGGAATTGGTCCACGTTATTGTCCAAGTGTTGAAGATAAAATTGTTAGATTTCAAGAAAAAGATACACATCATATTTTCTTCGAACCAGAAACAGCACGTGGTGACATCATGTACATTAATGGGTTGTCTACATCAATGCCTGAAGATGTTCAAGATGAAATGATTGCATCTATTCCAGGTCTTGAACATGCAAGAGTTCAAAAATATGGTTATGCAATTGAATATGATGCAATTAACCCTTTGAATTTATACAAATCTCTTGAAAGCAAAATTTTAAAAAATTTCTTTTCAGCAGGTCAACCAAATGGAACGAGTGGTTATGAAGAAGCAGCAGCTCAAGGATTAATAGCAGGAATTAACGCTGCTAATAAACTTGATAATTTAGAACCATTAATCATAAAAAGAAATGACGGTTATATTGGCGTTTTAATTGATGACATTGTAACTAAAGGCACAAATGAACCTTATAGAATGTTAACAAGCAGGGCAGAATTTAGACTTCTTTTGAGAAATGATAACGTTGATGAACGTTTGTATAAATATGCATATGACAACAAAATGATAAGCAAAGAAGAACATGATAGGATTGAAGAAAAATATAAGATTATTGCTGAAACAGTAGAAAAATTAAAAAATGAATATGTTTCTTCTAAAAGTGATGTTGGCCAAAAGTACAATGTAACAAATGGTATGTCATATTTAAAATTACTTTCAAATCCCGAAGTAGATCCAGTTGATATTTTAGGTTCAAATTTCCCATATATTGATGAAATAACTATTCAAGTTCGTTTGTATGGATATTTAAAGAAACAAGAATCAGCAGCTGATAAAATGCTTAGATTGGAACAATTAAAATTACCAAAAGACATTGATTATTGAAGTGTTGATAATTTAGCAACAGAGGCAAGACAAAAATTAGATAAAATAAGACCTGAAACAATTGGCCAAGCAGCAAGAATAAGTGGAATAAATCCTGCTGATATTCAAATGCTAATATTTTATTTAAACAATAAGCGTTAGTAATGAAATTAAATATTGTAGCAGTAGGTTCTCTAAGCAAAGAATATAAAACTATCTATGATCAATATCTTAAAAAAATTAGTTTTTTTTGTTCAATAAATTTGATTGAAATAAAAGAACAAGTTAATAAAAATATTGATTTAAAAATAGCCGAAGAAACAAAATTAATCCTAGAAAAAATTCCAAAGAACTCTAAAGTTTATTATTTGTCATTAAGAGGCAAGAAACTTGATAGCAATGAATTTTCGGATTTATTTATTGAAGATAATATTACTTTTGTAATAGGCGGTAGCAATGGAGTTGATGAAACATATTTTAAAAATAAAATATGTTTTTCAGATTTAACATTTCCTCATCAATTATTTAGAATAATGTTAATTGAACAAATTTATCGAGGATTTAGTATAAAAAACAACATAAAATATCACAAATAAAATAGGCTTTTATTTTAAAAAACAAGTCTATTTTTTTATTTCTTATTATTTTATTTAAAAGGAATAAAACATTTATAATTTAAATGTAACCATGTTAAATTTAAAAAATAAATTCTTGAAAGGAGTTTACATGGCAAAAAATACATGAGAAATTGCTAAACAAGCAATTGAAGAACACAATAATATTTTTATATTTCATCATATTAGACCTGACGGAGATTGTTTAGGCTCTCAATTTGGTTTGAGAGAATTAATAAAGGAAAATTATCCTGAAAAAAACGTTTTTGTTTTAGGGGATAATGGAGGAATCTTTCCATTTTTAGAATGAGATTTTGATAATTTTGAAAATATTGATAAAAAATATCTTAAGAATTCCTTAGGGGTAGTAGTTGATGCTAACTGCTCAAATAGAATTCAATTTGCTAATTATATTTTAGATAGTAATTTTACCCATATGTTAAGAATAGATCACCATCCAGTTGATCCAGATATTAATTATGACTATACTTGGGAAGATGCAACATATGCAGCAGCTGCTGAACAAGTTGGATATATCGCAATGATGGCAAAATGAAGAGTTACAACAAAAGCTGCTAAATACGTTTATTTGGGCATAAACACAGACTCTAATCGTTTTCAATATGACTATGTTCAAAAAAGAACATTTGATGTAGTAAGTTATCTTCATGAAGGCAATGATTTTAAAGTTTGAGACATTAACTTCCCTCTTTCAATTCGTGAAGAAAAGAAAGTTAGATTCAATGCCTATGTTCTTTTACATTACAAAAATGAAGGCAAAGTACTTTGATTCTATGCAAATAGGAAAATTCAAAAAAAATTTGGTTTGAATGAAAAAGAAGCAAATGATGTGGGAATTTTAGCAAATATTGGTGATGCTAAAATTTGAGTATTATTCATTGATGAACCAAATGGCAAAATTAGAGCAAGAGTAAGAAGCAATGGAATATGAATAAATCATATTTGTGCAAAACCTGAATATTACCCTGGTGGTGGTCACGAAGTTGCTTCAGGAGCAACTTGTGCTAATAAAAAATCTATGAAACATTTAATAGAAGACTTAAAGGCAGAGGTATTAAAATATGAATAAAATAAGTGCAGAAAAATTAGCAATTTTCAAGAAAATTGAAGAAAGAATTAAGGCACACAAAAATATAGTTATATATCATCATATTAGACCTGACGGAGATTGCTTGGGTTCTCAATTTGGAATGAAGAATTTAATTAAGGTAAATTTCCCTGATAAAACAGTGTATACAATTGGTGATTCCAAAGGTATTTATTCATTTTTAGAATTTAAAATGGATAAATTGCCACTAGAAAAGTTAGATGATTCATTAGCAATTATTGTTGATGCTAACTTTAAAGAACGTTTAGAATGTAGAGAATATTTAGACAATAATTGTTTTGATGAAGTAATAAGAATCGATCACCATCCAAATGATGATGATCTAGATGCTTCATTAAGATGAGTTGAGCCAGAAGCACCAGCAGCTGCTCAACAAGTAACTGAAATAGCATACGAATTAGGTTGAAAATTAAACAAAGAAGCTGCAACTTATTTATATTTGGGTATATATACTGATTCAGTTAAATTATCAACCAATACGACAACCGCAAAGACAATGTTGTTAGTAAGTTGATTGTGAGACAATGGTAGTCAAAAAGACTTAATTCATACAGAACTTTCTAAGCGTACATTATTTGATATCAACATAAACACATACATATCTCAAAACATGAAAATTGCAAACGACGTCGTTTCATTTTACTTCCCATTGTCTGAACAAATTAAATTAGGAATTAAAGATCCATTAAAAGCAAATAGACCTTTTGTTCTTGGCTCAATTGATAACACAAAGGCATGAGTATTCTTTACAGAAGAAAAACCTGGCCAAATTCGCTGTGAATTTAGATCAAACGGTTGCAATGTAAGAAACGTAGCAATCAAATGAGGCGGAGGTGGACATATACGTGCATCAGGCGCTCAAATTTCTGACCCTAACTTGATTCCGATGATTATTAAAGACCTAGAACAAGAAACAAAAAATCTTGCTGATTATGAATAAAAAATAGAAATAATTTGTTAAAAATGGCGATTCTTATTTTATAGGATCGTCCTTTTTTATTTTTTGACGGTAGAAGGCTAAAACCATTTAAAATAAACCAAAAAGTTTAAAAAAGTATTTTTTATTGCTAAAAAAGTATTCTTTTTTTTTTTTTTTTTAAGTTTGTTTCAAAATAAAGGAATTAAAAAACAATAAATTTATTTAGTTTAAATAAAAAAACAAAGGGAAAAAGGAACAACATGAAAACAAAATTAAAGGTTTTGCTTTCTGCTTCACTAATTACGGCAGTTGCAACAACGGCAGCAATATTTGCTACTGCTATAAAAAGTAAATTAAGAAAACAACACGATTTAAAGGAAAAGTATAACAATTTAAAATTGGAGTTAGAAAACTTAATTAAATTAGCAAAAATAGGCAATATTGAATCTTTGAATGAAATAAAAGCATTGAATAATTCAAAAATTGACAATAATTCAACAGTTAAAGAAATTGAAAATGCTACAAAGGAAATTGAAAAAGCAGTTGAATCAATTAATAAAAAAATTATTAATAAAAAGCATGATGATCAGATTCAACAAGAAATGGCAAATAAAGCTAAGCAAGAATTTATAGCCAAAAAGAATCAATTAAAAAAACTAATTAATTCTTCCCAATTAAGTAATATTGACAAGTTAAACGAATTAAAAAAGTTGAATGATATTAATATTAATGATAAAGATTTGGATGAAGAAATAAAAAATAAAACAAAAGCAATTGAAAAAAATATTGATTCATTGACTAAAAAAATAAATGAAAAGAAAGCCATAGAACAAAAAGCACAACAACTTCAAGAATTTGAAACCACTAAAAAGGCACTAGAAGAATTATTAAATTCTGAAGATGGCCAAAAAGTAAATAGTGATGATATAAAGAAAATATTGCAAAATAATAATGTAAACAATTCATCAACAAATACTCAAATTATTCAAAAAGGCAACGAAATCAAAAAAGCCAAACAAGATTTACAAAAATTAATCGATGATGCTAAAAATAAGGCAAAAAATGGTTTCGATGCTAAGAAGCAGGAATTGAAAAAGCTTTTGGATTCAACAACCGGTGAAATCAATAAGCAAACTGAAGATGAAGAGTATAAAAATATTGAAGTTAAAGGCAATGCTTCAATTAAAGATATTGAAAATAAAACAAAAGCAATTGAAACGGCAATTAATTCATTGACTAAAAAAATACAAAAAAGTCAATGTCTACAAAATTTTAATAATGCAAAAAGAATTTTAGAAGAATTTATTAAAAATGATGAAGATGCTAAAAATGATGAACTAATTTCAAATTTGAACGATGCTAAACAAGAATTAGCCAAATTTAATGTCATAAATGATTCACATGAAATCAATGAAATTAAAGAAGCTACTAATAGTTTAACAAATGCAAAAGCAAAATTGGAACAAGAAATTAAAAACAAAAAGCAAGAGTTGTTTAATGCGTTTGAATTATCTAAAACAGCATTGCAAAATTACATTACAAACGATATTAAAGATTCAAAATATAAAATTATTCAAGATAAATTTAATAATGTTATTGAAGAATATAAAAACATTACATCAAACGACAAAATTTCAAAAATTAAAGACGCTACAAACAATTTAAATGCAAAATTTATAGAAATTGGAAGCAACAAGGCAATATTAGATAAGTTTTATGAATTGAAGCCAAAACTTGAAGATTTATTAAACACCGCAAATCTTAAAGATGATGCAAAATCAGAAAATATTGATTTATCTTCTGAAGATCAAGCTTCTGCTGATGCCAATGCTATAAATAATAGTTCAACTATTGAAAATATTGAACAAGTTAACCAAAAATTACAAACAGCAATAGACAAATTAGCTAAAGATATTCAAAAAATAAATGACGATAAAAAAGCTAAATTTGAAGAATTTAAAACAGCAAAACAACATCTAGAAAACATAATCAAAACCAAAGGTTTAGATAAATTTATTAATATTGATGATGAAATAAAATCAATAAATCAAAAAATTAACGAAAAAATTAATGAATATAGTTCTATCAATCAAGATTCGAGAATTTTTGAAATAGATGATTCAAATAAAAAAATAGTAGCCTCTCATGAAGAATTTTCAAAACTATTGGATGATTATTTATCTAAGAGAAATGAATTAATTAGGAATCATAATACTGAGAAACAAGAATTACAAACATTAATTGATTCTGCAAAGGCAAAAAATGTTGATATTTCTGAGGAAATGAACATTGTAAGTAATGCAAATATTGGTGATGATGACCCTATTGATAACATTAGAAATAAAATAAATAAAATCAAAGATGCAATAAGCCAAATAAATTCAAAGATTAGAGCAAAAAAAGAACAAGCCAAAATAGAATATCAAAAATTCTTATCAATAAAGGAACGAGCAGAAAGCGTTATTGAGGAAATAAAGAAAATTCCTGATTATAAGCCTGAATATAACGAAAGAAAAACAGCAACAATAAATGAACTAAATCAATATTTATCTTCTTATAAAGGACTTTCTGAAAATTCAAATATAGAAGAAATATCTAAAGGAACACTTGATATTTCTAAGTGTTTAACATCATGAAGTGATTACAATTTTTCTACTGAAACTCAAAAATTTGTTGAAAATAATGTAGATCCATTTATAAAAGAAATTAATGAACTTAAGGGAAAACCAGATCAATTGGCAATGTATTGAAATGCTCAACATAAAAAACAAGACTTTGAAGGAATAAGATCAAATATTTTAAATTTAAAAGTTTTTGAAACCCAATCTAAGGAATTTGAAGATTTAAAATCAAAATTTGAGAATGAAAAAACAAAAACACTTAAAAAAATAAGAAGTTGGTACAAAAGGGCTATAAACGATAATAAAGAAACTTTGCATTTTGTGTCGTGACATATTGAAACTTATTTTAAAAACTACCCTGGACGCAAACAAGGCTATGAAGATTTATCTAAACTAATTGAAGAGATAAATAATGGATTAAAATCTTTAGATATGAACGATATAAAGAATTTAGATTGAATAAACGCAAAAGAATGAGAATTAAAGAAAAAACATAGACATTATTTAAAAGAATATGACCTTCTTAAAGAAGAAAAAAATTAAAAGCTAAAAACCAAGATTTGACAATACAACAAATCTTGGTTTTTAACTAGAATTAAATGCTAGCTATGAATAAAATTATCTAATATATAATTATCGACTTTCTTGTTTATTGATTTAATAAAAGAAAAATAAGTTTTGTATTTCTTAAAATCTCCCTTTCAAACAAGCTTTTTATTTAATGAATTTGCTAAATCAATTGCTAAATAGACATCAAAAATTCCAAATTCATTATGGCAGTCAATAATCATTTTTTCAATTAATGAATTTATTTCGTGGTCTGAAATATCGATATTATCTCTTTTATATGAGCTTAATGCTTTAGTAAATAAATACCCGAAGTATATGTTTCTACTTTGTTGATATTGTTTAAATTTTTCAATAAATTGCTTATGAATTTCATTTTCTTCTGGGCTTAGAGAAGCATTTGCAAAGAGCTTATCTGTATCGATTGCATTTTTTATATATACATTTTCTTTTTTTAATATTAATATCATTTTAATTACAATTAAAATGAATATAGCTAAGAAGATTGCACCAAAAACATAACAAACAATACTTGATTTTTTAGATATAGTTCCCATAATGCCATTTGCTGCTAAAAATAGCAAGATAGCAATAATAAGAAAGAGAGCAACGGTTAGAAAACCAATAATGTTCAATATAACATTTTTTATTTTCATTTTCTTTTCTTTTGATGTCATTTATTTAGCCTCCTTATAAGTTATTAGTTGAGGATTTTTTAAATATCCCCTTCCAGCTTTAGCGATATTATGGCCATTCAATCTTGTTGCATCGGCGCTAAAATTTACTCAAATTTTTAGCAGTGCTGCACCGATGCCGTATGTGTCAACAGGAGTCTCTTCGGCTTCAAATTCAGCAATTCTTTGAGCGTCAAATCCGCTTGAAACAGTTATTTTTACATGCTTGCCGTTATTATTGTCTAATGCTTCTCTTAGTCTTTTTACTTGATTTGGTGTAACGCCAAATTCTGCTTCGCCCTCCTTGAACATTTTATCAATAAGCGCTTTTGATGTGTCTATTCTTACACTTTTTAAGTCTTTGCCAAAAGCCTTTAAACATTTTAATGAATCGCTTATAACATCGTTGTGAAAATCAACTAAGGCAACTAGATCATCATTTGGAAAAACATTTTTATATGCTTTGCAAGCAGCTACAATATCACCGTCAAACATTTGAATTAATGCATGAGGCATGCTTCCAAAAGTATGCTTTGGATCAAAATTTGAGCTTAGAAAAGTCGAGTGATTTTCTATGCCGCCTATTCTTATTGCATATGAGTCGTTTGGTTGATTTATATAATGATCTGCCCTATCTGCCATGCTTACGATTTTTTTGCCATTTGCTGCCTTTATTGCTCAATATGCATTGGTTGCTATTGACGATTGTCTTGCTAAAATGCCATCAATGATTCCTTCTCATATACCAAATTCATAATAAGGGCCTTCAAGTTCTAAAACTACTTCTCTATTGTGGATTATACTGCCTTCTGGCATGTATCTTATGGTGTATTTAGAAATATCAGTGTTTTGTTTTAAAATATCAATTGCTTCATTTATTCCACATAATTTGACATCATTATGTCTTTGAAAGAATTGTAGAGTAATGTTTTGATCTTTTTTGAACTTTTCTGATATTTTTTGAGTTTTGTAAAAATAAATTGAAATGTAATCTTTCAAATTGATTTTTTTCATTATTCTCCTTTTTATAATAATTTAATAAATTATTATTAGCCTCTTATTAAAATTTTAAACAAAATTAATTAATAAAATCATATAAAGTTATAAAATAATAAATCATTGTTAAAATATCTTTTTTAAAGACATGTTTTATAATAAAGGAGAATAAGTATGGCAAAAAACCAAGATAAAAAAATGCTATCTCCAGAAGAAAAAGAAATAGAAAATATAAAGAAAATATCAAAAAATGGTTGACATTTTAGTTATTGATCGTTATTTTTTATTTTAGGAGTTATAGCTATTTTAATATGAGCAGTTGAATCTAAAAGTTCATCTTCAATGGTGAAATATCCATTATCTATAATATTTATAATTCTTTGCATAGCAATGATAGCTGGAATGTATACAATAAATGTAATGGGTTATAAACTAGCAATCAAATTAAACAATGAATCATTAAGAAAATGCTATTTGATTTCGCTAATTCCATTATTGTTTTTTGTAGCATTGGTAGCAAGCTTTGTATCTTCAATTAAGCTAAAAGAAATTTATATAGATAAAAAAAGCCAATTTCAAAACGTTGCTATAGCAGGATAGAAAAAAACCTTGAATTAATTTTCAAGGTTTTTTTGTTTAATTTCTTTTGCTATTTTGTTTGCATTTCTTTGGCGATGAGTAAATAGAATTATATAAAATACTAATGTAGGAATGGCAATGCTTATAAAGATTATAAAAAAGCAAGATAAGGCTATGTAACCATTTTGATTTCATAGGATGAAATAATTATCCAATGAAGTTATAGCATAAACACTTAATGTTGGAATAAACGCTATTGCAACTTGCGAAATTAAAGTGTGGCGTTTAGTTATTAATTGTGGCTTTTTCATCAATAGTATTGATGTTGTTAATATGTATGTAATTAAAATATAACCAACAATTACAAGTATTATAGGTAAAGTAATTTTCATAGTTAAAATTTTACTCCATTAAAAAATAAGAGCATTTAAAAAGCACATGTTTTAAACATGGCTTTTTAAAATTAGTTATT", "length": 715990, "complete": true, "type": "contig", "topology": "circular", "simple_id": "contig_1", "orig_id": "GCA_013368035.1|CP055150.1", "orig_description": "HMT-632 Metamycoplasma hominis SP3615" } ], "run": { "start": "2026-01-28 09:44:37", "end": "2026-01-28 09:52:03", "duration": "7.44 min" }, "version": { "bakta": "1.11.4", "db": { "version": "6.0", "type": "full" } } }